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30857546

Overweight trajectory and cardio metabolic risk factors in young adults.

BACKGROUND Obesity is one of the conditions that increases the risk of cardiovascular disease. Studies about obesity trajectory and cardio metabolic outcomes at adulthood are still scarce. Therefore, we aimed to assess the association between patterns of overweight over the life-course and cardio metabolic risk factors in young adults. METHODS In 1982, the maternity hospitals in Pelotas were visited daily and those newborns whose family lived in the urban area of the city were identified (n = 5914), and have prospectively followed for several occasions. Weight and height were measured at every visit. BMI-for-age z-score was calculated using the WHO Child Growth Standards. Overweight and obesity were defined as a BMI greater than or equal to 25 kg/m2 and 30 kg/m2 respectively. This was the definition adopted for evaluations overweight and obesity at 30 years. The participants were divided into eight groups according to the presence of overweight or obesity in childhood, adolescence and adulthood. Blood pressure, random blood glucose, HDL cholesterol, LDL cholesterol triglycerides and fat mass were measured. RESULTS From 2219 participants with anthropometric data in childhood, adolescence and adulthood, 25% never had been overweight, whereas 11.6% were overweight in the three periods. Random blood glucose, SBP and DBP were higher among those subjects who were always overweight/ obese or only overweight/obese during adolescence and adulthood. The participants who were never overweight/obese or only in childhood or adolescence had a lower cardiovascular risk profile (higher HDL cholesterol, lower blood pressure, lower random glucose, lower LDL cholesterol) at 30 years. Fat mass captured from 25 to 100% of the association of overweight and obesity trajectory with cardiometabolic risk factors. CONCLUSIONS The tracking of overweight/obesity is associated with an adverse cardio metabolic profile and this association is largely mediated by fat mass in adulthood.

['Callo Quinte|Gabriela|G|0000-0003-4479-2014', 'Barros|Fernando|F|', 'Gigante|Denise Petrucci|DP|', 'de Oliveira|Isabel Oliveira|IO|', 'Dos Santos Motta|Janaína Vieira|JV|', 'Horta|Bernardo Lessa|BL|']

2019

30324916

Itraconazole and thiophanate-methyl fail to clear tadpoles naturally infected with the hypervirulent lineage of Batrachochytrium dendrobatidis.

The emerging infectious disease chytridiomycosis, caused by the fungus Batrachochytrium dendrobatidis, is a major driver pushing many amphibian species to the brink of extinction. Substantial efforts to develop effective protocols that use antifungal drugs have had notable success. Here, we used the antifungal agents itraconazole and thiophanate-methyl, singly and in combination, in an attempt to treat common midwife toad Alytes obstetricans larvae naturally infected with the globalized hypervirulent lineage of B. dendrobatidis. Despite the successful use of itraconazole in a closely related species (A. muletensis), our results show that these antifungal treatments are not always effective and that full clearance of animals cannot be assumed following treatment.

['Fernández-Loras|Andrés|A|', 'Martín-Beyer|Bárbara|B|', 'Garner|Trenton W J|TWJ|', 'Bosch|Jaime|J|']

2018

32851593

Characterizing the fecal bacteria and archaea community of heifers and lactating cows through 16S rRNA next-generation sequencing.

The aim of this study was to describe the fecal bacteria and archaea composition of Holstein-Friesian and Simmental heifers and lactating cows, using 16S rRNA gene sequencing. Bacteria and archaea communities were characterized and compared between heifers and cows of the same breed. Two breeds from different farms were considered, just to speculate about the conservation of the microbiome differences between cows and heifers that undergo different management conditions. The two breeds were from two different herds. Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria were the most abundant phyla in all experimental groups. Alpha- and beta-diversity metrics showed significant differences between heifers and cows within the same breed, supported by principal coordinate analysis. The analysis of Holstein-Friesian fecal microbiome composition revealed 3 different bacteria families, 2 genera, and 2 species that differed between heifers and cows; on the other hand, Simmental heifers and cows differed only for one bacteria family, one archaeal genus, and one bacteria species. Results of the present study suggest that fecal communities of heifers and cows are different, and that fecal microbiome is maintained across experimental groups.

['Cendron|Filippo|F|http://orcid.org/0000-0002-8221-7566', 'Niero|Giovanni|G|', 'Carlino|Gabriele|G|', 'Penasa|Mauro|M|', 'Cassandro|Martino|M|']

2020

28601576

Real-time monitoring of microdistribution of antibody-photon absorber conjugates during photoimmunotherapy in vivo.

Photoimmunotherapy (PIT) is an emerging low side effect cancer therapy based on a monoclonal antibody (mAb) conjugated with a near-infrared (NIR) phthalocyanine dye IRDye 700DX. IR700 is fluorescent, can be used as an imaging agent, and also is phototoxic. It induces rapid cell death after exposure to NIR light. PIT induces highly selective cancer cell death, while leaving most of tumor blood vessels unharmed, leading to an effect called super-enhanced permeability and retention (SUPR). SUPR significantly improves the effectiveness of the anticancer drug. Currently, the therapeutic effects of PIT are monitored using the IR700 fluorescent signal based on macroscopic fluorescence reflectance imagery. This technique, however, lacks the resolution and depth information to reveal the intratumor heterogeneity of mAb-IR700 distribution. We applied a minimally invasive two-channel fluorescence fiber imaging system by combining the traditional fluorescence imaging microscope with two imaging fiber bundles (~0.85mm). This method monitored mAb-IR700 distribution and therapeutic effects during PIT at different intratumor locations (e.g., tumor surface vs. deep tumor) in situ and in real time simultaneously. This enabled evaluation of the therapeutic effects in vivo and treatment regimens. The average IR700 fluorescence intensity recovery after PIT to the tumor surface is 91.50%, while it is 100.63% in deep tumors. To verify the results, two-photon microscopy combined with a microprism was also used to record the mAb-IR700 distribution and fluorescence intensity of green fluorescent protein (GFP) at different tumor depths during PIT. After PIT treatment, there was significantly higher IR700 fluorescence recovery in deep tumor than in the tumor surface. This phenomenon can be explained by increased vascular permeability immediately after NIR-PIT. Fluorescence intensity of GFP at the tumor surface decreased significantly more compared to that of deep tumor and in controls (no PIT).

['Tang|Qinggong|Q|', 'Nagaya|Tadanobu|T|', 'Liu|Yi|Y|', 'Lin|Jonathan|J|', 'Sato|Kazuhide|K|', 'Kobayashi|Hisataka|H|', 'Chen|Yu|Y|']

2017

29536177

Stable bioemulsifiers are produced by Acinetobacter bouvetii UAM25 growing in different carbon sources.

Acinetobacter species are identified as producing surface-active and emulsifying molecules known as bioemulsifiers. Production, characterization and stability of bioemulsifiers produced by Acinetobacter bouvetii UAM25 were studied. A. bouvetii UAM25 grew in three different carbon and energy sources: ethanol, a glycerol-hexadecane mixture and waste cooking oil in an airlift bioreactor, showing that bioemulsifier production was growth associated. The three purified bioemulsifiers were lipo-heteropolysaccharides of high molecular weight (4866 ± 533 and 462 ± 101 kDa). The best carbon source and energy for bioemulsifier production was wasted cooking oil, with a highest emulsifying capacity (76.2 ± 3.5 EU mg-1) as compared with ethanol (46.6 ± 7.1 EU mg-1) and the glycerol-hexadecane mixture (49.5 ± 4.2 EU mg-1). The three bioemulsifiers in our study displayed similar macromolecular structures, regardless of the nature (hydrophobic or hydrophilic) of the carbon and energy source. Bioemulsifiers did not decrease surface tension, but the emulsifying capacity of all of them was retained under extreme variation in salinity (0-50 g NaCl L-1), pH (3-10) and temperature (25-121 °C), indicative of remarkable stability. These findings contribute to understanding of the relationship between: production, physical properties, chemical composition and stability of bioemulsifiers for their potential applications in biotechnology, such as bioremediation of hydrocarbon-contaminated soil and water.

['Ortega-de la Rosa|Nestor D|ND|', 'Vázquez-Vázquez|Jose L|JL|', 'Huerta-Ochoa|Sergio|S|', 'Gimeno|Miquel|M|', 'Gutiérrez-Rojas|Mariano|M|http://orcid.org/0000-0001-8285-8200']

2018

27664491

Resveratrol Supplementation and Oxidative/Anti-Oxidative Status in Patients with Ulcerative Colitis: A Randomized, Double-Blind, Placebo-controlled Pilot Study.

BACKGROUND AND AIMS Oxidative stress is involved in both pathogenesis and exacerbation of ulcerative colitis (UC). This study was designed to evaluate whether resveratrol, an excellent anti-oxidant agent, can help in treatment of UC and its related oxidative stress. METHODS AND RESULTS Fifty six patients with active mild to moderate disease were randomized to receive either 500 mg/day resveratrol capsules or the same amount of placebo for 6 weeks. Before and after the intervention, disease activity, quality of life, and oxidative stress were assessed using the Simple Clinical Colitis Activity Index Questionnaire (SCCAIQ), Inflammatory Bowel Disease Questionnaire-9 (IBDQ-9), and serum level of malondialdehyde (MDA), superoxide dismutase (SOD), and total anti-oxidant capacity (TAC), respectively. Serum SOD (122.28 ± 11.55 to 125.77 ± 10.97) and TAC (9.87 ± 1.51-11.97 ± 1.61) increased, whereas serum MDA (5.62 ± 1.18-3.42 ± 1.01) decreased significantly in resveratrol group (p <0.001). Moreover, resveratrol supplementation significantly decreased disease activity and increased the quality of life (p <0.001). CONCLUSION Our data indicate that 500 mg/day resveratrol supplementation can improve the disease activity and quality of life in patients with UC at least partially through reduction of oxidative stress. Further studies are needed to determine the optimal dosage of supplementation for these patients.

['Samsamikor|Maryam|M|', 'Daryani|Naser Ebrahimi|NE|', 'Asl|Parisa Rezanejad|PR|', 'Hekmatdoost|Azita|A|']

2016

26333017

Mapping the electrophysiological and morphological properties of CA1 pyramidal neurons along the longitudinal hippocampal axis.

Differences in behavioral roles, anatomical connectivity, and gene expression patterns in the dorsal, intermediate, and ventral regions of the hippocampus are well characterized. Relatively fewer studies have, however, focused on comparing the physiological properties of neurons located at different dorsoventral extents of the hippocampus. Recently, we reported that dorsal CA1 neurons are less excitable than ventral neurons. There is little or no information for how neurons in the intermediate hippocampus compare to those from the dorsal and ventral ends. Also, it is not known whether the transition of properties along the dorsoventral axis is gradual or segmented. In this study, we developed a statistical model to predict the dorsoventral position of transverse hippocampal slices. Using current clamp recordings combined with this model, we found that CA1 neurons in dorsal, intermediate, and ventral hippocampus have distinct electrophysiological and morphological properties and that the transition in most (but not all) of these properties from the ventral to dorsal end is gradual. Using linear and segmented regression analyses, we found that input resistance and resting membrane potential changed linearly along the V-D axis. Interestingly, the transition in resonance frequency, rebound slope, dendritic branching in stratum radiatum, and action potential properties was segmented along the V-D axis. Together, the findings from this study highlight the heterogeneity in CA1 neuronal properties along the entire longitudinal axis of hippocampus.

['Malik|Ruchi|R|', 'Dougherty|Kelly Ann|KA|', 'Parikh|Komal|K|', 'Byrne|Connor|C|', 'Johnston|Daniel|D|']

2016

34209988

Antimicrobial and Antibiofilm Activity of the Probiotic Strain Streptococcus salivarius K12 against Oral Potential Pathogens.

Oral probiotics are increasingly used in the harmonization of the oral microbiota in the prevention or therapy of various oral diseases. Investigation of the antimicrobial activity of the bacteriocinogenic strain Streptococcus salivarius K12 against oral pathogens shows promising results, not only in suppressing growth, but also in eliminating biofilm formation. Based on these findings, we decided to investigate the antimicrobial and antibiofilm activity of the neutralized cell-free supernatant (nCFS) of S. salivarius K12 at various concentrations against selected potential oral pathogens under in vitro conditions on polystyrene microtiter plates. The nCFS of S. salivarius K12 significantly reduced growth (p < 0.01) in Streptococcus mutans Clarke with increasing concentration from 15 to 60 mg/mL and also in Staphylococcus hominis 41/6 at a concentration of 60 mg/mL (p < 0.001). Biofilm formation significantly decreased (p < 0.001) in Schaalia odontolytica P10 at nCFS concentrations of 60 and 30 mg/mL. Biofilm inhibition (p < 0.001) was also observed in Enterobacter cloacae 4/2 at a concentration of 60 mg/mL. In Schaalia odontolytica P10 and Enterobacter cloacae 4/2, the nCFS had no effect on their growth.

['Stašková|Andrea|A|', 'Sondorová|Miriam|M|', 'Nemcová|Radomíra|R|', 'Kačírová|Jana|J|0000-0002-4252-1774', 'Maďar|Marián|M|']

2021

25847161

A highly selective molecularly imprinted electrochemiluminescence sensor for ultra-trace beryllium detection.

A new molecularly imprinted electrochemiluminescence (ECL) sensor was proposed for highly sensitive and selective determination of ultratrace Be(2+) determination. The complex of Be(2+) with 4-(2-pyridylazo)-resorcinol (PAR) was chosen as the template molecule for the molecularly imprinted polymer (MIP). In this assay, the complex molecule could be eluted from the MIP, and the cavities formed could then selectively recognize the complex molecules. The cavities formed could also work as the tunnel for the transfer of probe molecules to produce sound responsive signal. The determination was based on the intensity of the signal, which was proportional to the concentrations of the complex molecule in the sample solution, and the Be(2+) concentration could then be determined indirectly. The results showed that in the range of 7×10(-11 )mol L(-1) to 8.0×10(-9) mol L(-1), the ECL intensity had a linear relationship with the Be(2+) concentrations, with the limit of detection of 2.35×10(-11) mol L(-1). This method was successfully used to detect Be(2+) in real water samples.

['Li|Jianping|J|', 'Ma|Fei|F|', 'Wei|Xiaoping|X|', 'Fu|Cong|C|', 'Pan|Hongcheng|H|']

2015

23910251

A review on nickel-free nitrogen containing austenitic stainless steels for biomedical applications.

The field of biomaterials has become a vital area, as these materials can enhance the quality and longevity of human life. Metallic materials are often used as biomaterials to replace structural components of the human body. Stainless steels, cobalt-chromium alloys, commercially pure titanium and its alloys are typical metallic biomaterials that are being used for implant devices. Stainless steels have been widely used as biomaterials because of their very low cost as compared to other metallic materials, good mechanical and corrosion resistant properties and adequate biocompatibility. However, the adverse effects of nickel ions being released into the human body have promoted the development of "nickel-free nitrogen containing austenitic stainless steels" for medical applications. Nitrogen not only replaces nickel for austenitic structure stability but also much improves steel properties. Here we review the harmful effects associated with nickel and emphatically the advantages of nitrogen in stainless steel, as well as the development of nickel-free nitrogen containing stainless steels for medical applications. By combining the benefits of stable austenitic structure, high strength, better corrosion and wear resistance and superior biocompatibility in comparison to the currently used austenitic stainless steel (e.g. 316L), the newly developed nickel-free high nitrogen austenitic stainless steel is a reliable substitute for the conventionally used medical stainless steels.

['Talha|Mohd|M|', 'Behera|C K|CK|', 'Sinha|O P|OP|']

2013

28593631

Bivalirudin versus heparin and provisional GP IIb/IIIa inhibitors in patients treated for ST-segment elevation myocardial infarctions: Comparison of outcomes in a "real-world" setting.

INTRODUCTION The beneficial effects of bivalirudin during primary PCIs are controversially discussed, data on unselected patients are rare. It was the aim of the study to compare bivalirudin versus heparin and provisional glycoprotein IIb/IIIa inhibitors (GPIs) in a "real-world" study. METHODS From 05/2013 until 11/2014, the STEMI-patients in the Bremen STEMI registry were treated with periinterventional bivalirudin; before and after this period the standard anticoagulative treatment was heparin and provisional GPIs. RESULTS In 714 patients bivalirudin was used for PCI, this cohort was compared to 683 patients with heparin and provisional GPIs. In patients with bivalirudin a significantly lower rate of hospital bleedings was observed compared to patients with heparin (4.6% vs 8.1%, P < 0.01, multivariate HR 0.57, 95%CI 0.35-0.93), in an exclusive analysis of severe bleedings a trend toward less bleedings was found in patients with bivalirudin (2.0% vs 3.5%, P = 0.07, multivariate HR 0.66, 95%CI 0.30-1.42). The rate of stent thromboses reinfarctions and mortality was not different between the bivalirudin and the heparin group. During 1-year follow-up bivalirudin was associated with a lower rate of bleedings and no significant differences in stent thromboses, reinfarctions, and mortality. Bivalirudin was not associated with an excess of bleedings or stent thromboses in subgroups that are regularly underrepresented in randomized trials (older patients, women, cardiogenic shock). CONCLUSIONS In this "real-world" cohort of patients with STEMI bivalirudin compared to heparin and GPIs was associated with less bleedings and no significant differences in stent thromboses, reinfarctions, and mortality during hospital and long-term course.

['Fach|Andreas|A|', 'Backhaus|Tina|T|', 'Schmucker|Johannes|J|', 'Zabrocki|Robert|R|', 'Garstka|Daniela|D|', 'Stehmeier|Janina|J|', 'Fiehn|Eduard|E|', 'Hambrecht|Rainer|R|', 'Wienbergen|Harm|H|http://orcid.org/0000-0001-5603-9391']

2017

24434702

Efficient conversion of brown grease produced by municipal wastewater treatment plant into biofuel using aluminium chloride hexahydrate under very mild conditions.

Wastes produced by oil/water separation at the wastewater treatment plant of Bari West (Southern Italy) were taken, characterized and converted. About 12% of this material was composed of greases, mainly made of free fatty acids (50%) and soaps (34%), and was easily separable by the aqueous phase through a hot centrifugation. After chemical activation of this fatty fraction, a direct esterification was carried out under very mild conditions (320K and atmospheric pressure), converting more than 90% of the original free fatty acids into the respective methyl esters in less than 4h, by using AlCl3·6H2O. The activation energy correlated to the use of this catalyst was also calculated (Eaest=43.9kJmol(-1)). The very low cost of the biodiesel produced (0.45€L(-1)) and the associated relevant specific energy (5.02MJ kgFAMEs(-1)) make such a process a really sustainable and effective example of valorization of a waste.

['Pastore|Carlo|C|', 'Lopez|Antonio|A|', 'Mascolo|Giuseppe|G|']

2014

25084838

The osmotic resistance, and zeta potential responses of human erythrocytes to transmembrane modification of Ca2+ fluxes in the presence of the imposed low rate radiation field of 90Sr.

PURPOSE To investigate the effects of the imposed low dose rate ionizing field on membrane stability of human erythrocytes under modulation of transmembrane exchange of Ca(2+). MATERIALS AND METHODS Osmotic resistance of human erythrocytes was determined by a measure of haemoglobin released from erythrocytes when placed in a medium containing serial dilutions of Krebs isotonic buffer. The zeta potential as indicator of surface membrane potential was calculated from value of the cellular electrophoretic mobility. The irradiation of erythrocyte suspensions carried out by applying suitable aliquots of (90)Sr in incubation media. RESULTS Irradiation of human erythrocytes by (90)Sr (1.5-15.0 μGy·h(-1)) induced a reversible increase of hyposmotic hemolysis and negative charge value on the outer membrane surface as well as changed responses these parameters to modification of Ca(2+) fluxes with calcimycin and nitrendipine. CONCLUSIONS Findings indicate that the low dose rate radionuclides ((90)Sr) field modifies both Ca(2+)-mediated, and Ca(2+)-independent cellular signalling regulating mechanical stability of erythrocyte membrane. A direction of that modification presumably depends on the initial structure of membranes, and it is determined by the quality and quantitative parameters of changes in membrane structure caused by concrete operable factors.

['Zhirnov|Victor V|VV|', 'Iakovenko|Igor N|IN|']

2015

27555433

Dysfibrinogenemia-associated novel heterozygous mutation, Shanghai (FGA c.169_180+2 del), leads to N-terminal truncation of fibrinogen Aα chain and impairs fibrin polymerization.

AIMS A novel heterozygous variant, FGA c.169_180+2 del (designated fibrinogen Shanghai), was identified in a patient with dysfibrinogenemia with antiphospholipid antibody syndrome (APS) and recurrent venous thrombosis, and in his asymptomatic father. We aimed to reveal the functional implication of structural change caused by this variant. METHODS Transcription analysis was performed with FGA minigene transfection assay to evaluate the impact of nucleosides deletion on mRNA editing. The fibrinogen isolated from propositus' plasma was used to characterise its functional defects. Fibrin polymerization and clot lysis experiments were performed by optical measurement of turbidity. Thrombin-catalysed fibrinopeptide release was analysed by the reversed-phase, high-performance liquid chromatography. The ultrastructures of fibrin clots were visualised by scanning electron microscopy. RESULTS FGA c.169_180+2 del led to an aberrant mRNA with exon 2 skipping and encoded an shortened Aα chain with 42 amino acids truncation at its N-terminal. The propositus' fibrinogen had an impaired release of fibrinopeptide A and abnormal polymerization with a significantly prolonged lag time, a slower maximum slope and reduced final turbidity. The fibrin clot formed with propositus' fibrinogen showed thicker fibres with looser network structure. Clot lysis was normal using the purified fibrinogen but was significantly impaired using the plasma sample from propositus, compared with that from his father. CONCLUSIONS Fibrinogen Shanghai results in N-terminal truncation of Aα chain, which does not interfere with synthesis, assembly or secretion of fibrinogen, but compromises fibrin polymerization and clot formation. APS at least partially contributes to the development of thrombosis in the propositus.

['Zhou|Jingyi|J|', 'Ding|Qiulan|Q|', 'Wu|Wenman|W|', 'Ouyang|Qi|Q|', 'Xie|Yinyin|Y|', 'Wu|Xi|X|', 'Lu|Yeling|Y|', 'Dai|Jing|J|', 'Liang|Qian|Q|', 'Wang|Hongli|H|', 'Wang|Xuefeng|X|', 'Hu|Yiqun|Y|']

2017

31796271

Modification by earthworms of effects of soil heterogeneity and root foraging in eight species of grass.

Spatial heterogeneity of soil nutrients and earthworm activity can each increase the performance of plant species, but their interactive effects have been little studied. The ability of plants to forage for nutrients by concentrating roots where nutrients are concentrated can partly explain the positive effects of nutrient heterogeneity, but whether root foraging can help explain the positive effects of earthworm activity is untested. We conducted a greenhouse experiment in which we grew eight species of Poaceae in homogeneous and heterogeneous soils with or without the earthworms Eisenia fetida and Metaphire guillelmi and measured net accumulation of plant mass and tillers. Effects of heterogeneity and earthworms on plant performance were positive in most species. The presence of earthworms reduced the directly measured effect of heterogeneity on total mass in some grass species. Most species showed root foraging ability. Ability showed no relationship to effects of heterogeneity or earthworms on final total dry mass. However, earthworms reduced foraging in some species, possibly by lessening heterogeneity. Earthworm activity in heterogeneous soil may thus reduce the benefits of root foraging for nutrients in plants.

['Liu|Lu|L|', 'Alpert|Peter|P|', 'Dong|Bi-Cheng|BC|', 'Yu|Fei-Hai|FH|']

2020

25555771

Righting the mismatch between law, policy and the sexual and reproductive health needs of young people in the Asia-Pacific Region.

The context of sexual relations is changing in the Asia-Pacific. While the age of sexual debut remains the same, young people are generally marrying later and sex outside of marriage is increasing. The first systematic review of how laws and policies govern young people's access to sexual and reproductive health services was conducted in 2013. The study considered >400 national documents and held focus group discussions with >60 young people across three countries in the region. This paper examines the study findings in light of epidemiological data on young people's sexual behaviour and health, exposing a critical mismatch between the onset of sexual activity and laws and policies governing consent (to sex and medical treatment), and the restriction and orientation of services to married persons. An enabling legal and policy environment is an essential foundation for efforts to improve young people's sexual and reproductive health. This paper argues that international guidance and commitments (including the widely ratified Convention on the Rights of the Child) provide a framework for recognising young people's evolving capacity for independent decision-making, including in the realm of sexual and reproductive health. A number of countries in the region are using these frameworks to expand access to services, providing valuable examples for others to build on.

['Godwin|John|J|', 'Szabo|Gabrielle|G|', 'Sass|Justine|J|', 'Sauvarin|Josephine|J|']

2014

32885839

Innovative transcriptome-based genotyping highlights environmentally responsive genes for phenology, growth and yield in a non-model grain legume.

The narrow-leafed lupin, Lupinus angustifolius L., is a grain legume crop, cultivated both as a green manure and as a source of protein for animal feed and human food production. During its domestication process, numerous agronomic traits were improved, however, only two trait-related genes were identified hitherto, both by linkage mapping. Genome-wide association studies (GWAS), exploiting genomic sequencing, did not select any novel candidate gene. In the present study, an innovative method of 3'-end reduced representation transcriptomic profiling, a massive analysis of cDNA ends, has been used for genotyping of 126 L. angustifolius lines surveyed by field phenotyping. Significant genotype × environment interactions were identified for all phenology and yield traits analysed. Principal component analysis of population structure evidenced European domestication bottlenecks, visualized by clustering of breeding materials and cultivars. GWAS provided contribution towards deciphering vernalization pathway in legumes, and, apart from highlighting known domestication loci (Ku/Julius and mol), designated novel candidate genes for L. angustifolius traits. Early phenology was associated with genes from vernalization, cold-responsiveness and phosphatidylinositol signalling pathways whereas high yield with genes controlling photosynthesis performance and abiotic stress (drought or heat) tolerance. PCR-based toolbox was developed and validated to enable tracking desired alleles in marker-assisted selection. Narrow-leafed lupin was genotyped with an innovative method of transcriptome profiling and phenotyped for phenology, growth and yield traits in field. Early phenology was found associated with genes from cold-response, vernalization and phosphatidylinositol signalling pathways, whereas high yield with genes running photosystem II and drought or heat stress response. Key loci were supplied with PCR-based toolbox for marker-assisted selection.

['Plewiński|Piotr|P|', 'Ćwiek-Kupczyńska|Hanna|H|', 'Rudy|Elżbieta|E|', 'Bielski|Wojciech|W|', 'Rychel-Bielska|Sandra|S|', 'Stawiński|Stanisław|S|', 'Barzyk|Paweł|P|', 'Krajewski|Paweł|P|', 'Naganowska|Barbara|B|', 'Wolko|Bogdan|B|', 'Książkiewicz|Michał|M|0000-0002-3382-8504']

2020

30260540

Alterations in intrinsic fronto-thalamo-parietal connectivity are associated with cognitive control deficits in psychotic disorders.

Despite a growing number of reports about alterations in intrinsic/resting brain activity observed in patients with psychotic disorders, their relevance to well-established cognitive control deficits in this patient group is not well understood. Totally 88 clinically stabilized patients with a psychotic disorder and 50 healthy controls participated in a resting-state magnetic resonance imaging study (rs-MRI) and performed an antisaccade task in the laboratory to assess voluntary inhibitory control ability. Deficits on this task are a well-established biomarker across psychotic disorders as we found in the present patient sample. First, regional cerebral function was evaluated by measuring the amplitude of low frequency fluctuations (ALFF) in rs-MRI BOLD signals. We found reduced ALFF in patients in regions known to be relevant to antisaccade task performance including bilateral frontal eye fields (FEF), supplementary eye fields (SEF) and thalamus. Second, areas with ALFF alterations were used as seed areas in whole-brain functional connectivity (FC) analysis. Altered FC was observed in a fronto-thalamo-parietal network that was associated with inhibition error rate in patients but not in controls. In contrast, faster time to generate a correct antisaccade was associated with FC in FEF and SEF in controls but this effect was not seen in patients. These findings establish a behavioral relevance of resting-state fMRI findings in psychotic disorders, and extend previous reports of alterations in fronto-thalamo-parietal network activation during antisaccade performance seen in task-based fMRI studies.

['Lencer|Rebekka|R|', 'Yao|Li|L|', 'Reilly|James L|JL|', 'Keedy|Sarah K|SK|', 'McDowell|Jennifer E|JE|', 'Keshavan|Matcheri S|MS|', 'Pearlson|Godfrey D|GD|', 'Tamminga|Carol A|CA|', 'Gershon|Elliot S|ES|', 'Clementz|Brett A|BA|', 'Lui|Su|S|0000-0003-3541-1769', 'Sweeney|John A|JA|']

2019

25555349

Mechanism of subunit coordination of an AAA+ hexameric molecular nanomachine.

Simian virus 40 large tumor antigen (LT) is both a potent oncogenic protein and an efficient hexameric nanomachine that harnesses the energy from ATP binding/hydrolysis to melt origin DNA and unwind replication forks. However, how the six subunits of the helicase motor coordinate during ATP hydrolysis and DNA unwinding/translocation is unresolved. Here we investigated the subunit coordination mechanisms "binomial distribution mutant doping" experiments in the presence of various DNA substrates. For ATP hydrolysis, we observed multiple coordination modes, ranging from random and semi-random, and semi-coordinated modes, depending on which type of DNA is present. For DNA unwinding, however, the results indicated a fully-coordinated mode for the natural origin-containing duplex DNA, but a semi-coordinated mode for a pre-existing fork-DNA, providing direct evidence for LT to use potentially different mechanisms to unwind the two types of substrates. The results of this study provide insights into DNA translocation and unwinding mechanisms for LT hexameric biomotor. From the clinical editor: The study describes the subunit coordination of simian virus 40 large tumor antigen (LT) showing that multiple mechanisms exist that handle the specific needs of different stages of DNA replication.

['Yu|Xian Jessica|XJ|', 'Greenleaf|William B|WB|', 'Shi|Yemin Stanley|YS|', 'Chen|Xiaojiang S|XS|']

2015

25443472

Pseudomonas aeruginosa in CF and non-CF homes is found predominantly in drains.

BACKGROUND For patients with cystic fibrosis (CF) Pseudomonas aeruginosa infection is a major contributor to progressive lung disease. While colonizing strains are thought to be primarily environmental, which environments are important in lung colonization is unclear. METHODS We took 11,674 samples from a broad range of sites over 3-8 visits to homes with (7) and without (8) CF patients. RESULTS Twenty-eight percent of sampled drains yielded P. aeruginosa at least once, and a general mixed linear model estimated that 6.3% of samples from drains yield P. aeruginosa. This is more than eight times the estimated recovery from any other type of household environment. CONCLUSIONS These findings implicate drains as important potential sources of P. aeruginosa infection. They suggest that maximizing P. aeruginosa control efforts for drains would reduce exposure with minimal extra burden to CF patients and families.

['Purdy-Gibson|M E|ME|', 'France|M|M|', 'Hundley|T C|TC|', 'Eid|N|N|', 'Remold|S K|SK|']

2015

27085537

CD30+ Cutaneous T Cell Lymphoma: Response to Rotational Total Skin Electron Irradiation.

INTRODUCTION Rotational total skin electron irradiation (RTSEI) is an effective therapy for cutaneous T cell lymphoma (CTCL). CD30 expression has been identified as a prognostic factor in CTCL. Therefore, we investigated CD30 status, treatment response, and survival in our cohort of patients with CTCL treated with RTSEI. METHODS Patients with CTCL treated with RTSEI (≥30 Gy) between 2000 and 2013 at our institution were identified, and clinical and pathologic data were retrospectively reviewed. Primary outcomes were complete clinical response (CCR; >90% reduction of skin disease burden), relapse-free survival (RFS), and overall survival (OS). RESULTS Sixty-eight patients with CTCL treated with RTSEI were identified. Median age at diagnosis was 51 years with median follow-up of 61 months. Median OS was 76 months and median RFS was 11 months. Thirteen patients (19%) had CD30+ lymphocytes on initial pathology. In the CD30+ cohort, there were no T2, eight T3, and five T4 cases. In comparison, in the CD30- cohort, there were 18 T2, 29 T3, and 8 T4 cases (P = 0.01). Six weeks post-RTSEI, CCR was 85% in CD30+ and 81% in CD30- cases (P = 1). Six months post-RTSEI, CCR was 23% in CD30+ and 50% in CD30- cases (P = 0.083). CONCLUSION RTSEI resulted in excellent CCR at 6 weeks in our cohort of patients with CTCL, with a median RFS of 11 months. We found CD30+ patients presented with significantly higher T stage at time of RTSEI and trended towards decreased CCR at 6 months post-RTSEI compared with the CD30- group.

['Danish|Hasan H|HH|', 'Heumann|Thatcher R|TR|', 'Bradley|Kyle T|KT|', 'Switchenko|Jeffrey|J|', 'Esiashvili|Natia|N|', 'Lechowicz|Mary Jo|MJ|', 'Flowers|Christopher R|CR|', 'Khan|Mohammad K|MK|']

2016

23838302

A comparative study of volatile components in green, oolong and black teas by using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry and multivariate data analysis.

The difference of volatile components in green, oolong and black teas was studied by using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS). Simultaneous distillation extraction was proved to be a suitable technique to extract the analytes with interest. A total of 450 compounds were tentatively identified with comparison to the standard mass spectra in available databases, retention index on the first dimension and structured chromatogram. 33 tea samples, including 12, 12 and 9 samples of green, oolong and black tea were analyzed by using GC×GC-TOFMS. After peak alignment, around 3600 peaks were detected. Partial least squares - discriminant analysis and hierarchical cluster analysis were used to classify these samples, then non-parametric hypothesis test (Mann-Whitney U test) and the variable importance in the projection (VIP) were applied to discover the key components to distinguish the three types of tea with significant difference amongst them. 74 differential compounds are defined to interpret the chemical differences of 3 types of tea. This study shows the power of GC×GC-TOFMS method combined with multivariate data analysis to investigate natural products with high complexity for information extraction.

['Zhang|Lei|L|', 'Zeng|Zhongda|Z|', 'Zhao|Chunxia|C|', 'Kong|Hongwei|H|', 'Lu|Xin|X|', 'Xu|Guowang|G|']

2013

33822064

The stability of an isolate of the SARS-CoV-2 B.1.1.7 lineage in aerosols is similar to three earlier isolates.

BACKGROUND Our laboratory previously examined the influence of environmental conditions on the stability of an early isolate of SARS-CoV-2 (hCoV-19/USA/WA-1/2020) in aerosols generated from culture medium or simulated saliva. However, genetic differences have emerged among SARS-CoV-2 lineages, and it is possible that these differences may affect environmental stability and the potential for aerosol transmission. METHODS The influence of temperature, relative humidity, and simulated sunlight on the decay of four SARS-CoV-2 isolates in aerosols, including one belonging to the recently emerged B.1.1.7 lineage, were compared in a rotating drum chamber. Aerosols were generated from simulated respiratory tract lining fluid to represent aerosols originating from the deep lung. RESULTS No differences in the stability of the isolates were observed in the absence of simulated sunlight at either 20°C or 40°C. However, a small but statistically significant difference in the stability was observed between some isolates in simulated sunlight at 20°C and 20% relative humidity. . CONCLUSIONS The stability of SARS-CoV-2 in aerosols does not vary greatly among currently circulating lineages, including B.1.1.7, suggesting that the increased transmissibility associated with recent SARS-CoV-2 lineages is not due to enhanced survival in the environment.

['Schuit|Michael|M|', 'Biryukov|Jennifer|J|', 'Beck|Katie|K|', 'Yolitz|Jason|J|', 'Bohannon|Jordan|J|', 'Weaver|Wade|W|', 'Miller|David|D|', 'Holland|Brian|B|', 'Krause|Melissa|M|', 'Freeburger|Denise|D|', 'Williams|Gregory|G|', 'Wood|Stewart|S|', 'Graham|Amanda|A|', 'Rosovitz|M J|MJ|', 'Bazinet|Adam|A|', 'Phillips|Aaron|A|', 'Lovett|Sean|S|', 'Garcia|Karla|K|', 'Abbott|Elyse|E|', 'Wahl|Victoria|V|', 'Ratnesar-Shumate|Shanna|S|', 'Dabisch|Paul|P|']

2021

27162189

An individualized exercise programme with and without behavioural change enhancement strategies for managing fatigue among frail older people: a quasi-experimental pilot study.

OBJECTIVE To evaluate the feasibility and preliminary effects of an individualized exercise programme with and without behavioural change enhancement strategies for frail older people with fatigue. DESIGN A three-arm, single-blinded, quasi-experimental pilot study. SETTING Community health centres. PARTICIPANTS A total of 79 frail older people with fatigue, mean age 79.32 years (±7.72). INTERVENTIONS The combined group received a 16-week combined intervention consisting of exercise training and a behavioural change enhancement programme. The exercise group received exercise training and health talks, whereas the control group received only health talks. MAIN OUTCOME MEASURE(S) Feasibility was assessed through the participants' recruitment, retention, attendance and adherence, feedback, and reports of adverse events. The preliminary effects were assessed by the participants' level of fatigue, physical endurance, self-efficacy, and self-perceived compliance with exercise. RESULTS Feasibility was achievable with high recruitment (87.2%) and low overall attrition (7.1%) rates. A similar reduction in fatigue was identified in all groups, but a trend of greater improvement in physical endurance was observed in the combined group than in the other two groups. The combined group also had a significantly better attendance rate [F(2,76) = 5.64, p < 0.01)] and higher self-perceived exercise compliance than the exercise group. CONCLUSION The combined intervention has the potential to enhance the participants' adherence to exercise regimens by improving their attendance in training sessions and their self-perceived exercise compliance. They are important to maintaining an appropriate level of engagement in daily exercises, especially at the beginning stages of behavioural change, when the participants are establishing the habit of exercising daily.

['Liu|Justina Y-W|JY|', 'Lai|Claudia Ky|CK|', 'Siu|Parco M|PM|', 'Kwong|Enid|E|', 'Tse|Mimi My|MM|']

2017

31439254

7T TOF-MRA shows modulated orifices of lenticulostriate arteries associated with atherosclerotic plaques in patients with lacunar infarcts.

PURPOSE To characterize the orifices of lenticulostriate arteries (LSAs) in vivo by using three-dimensional (3D) time-of-flight magnetic resonance angiography (TOF-MRA) and to investigate the spatial relationship between LSA orifices and atherosclerotic plaques in patients with lacunar infarcts (LI). METHOD Seventeen healthy volunteers and fifteen patients with LI underwent 3D TOF-MRA and 3D vessel wall imaging (VWI) at 7 T. The orifices of LSAs and the locations of atherosclerotic plaques on MCA walls were categorized based on the involvement of the superior, inferior, ventral or dorsal sides of MCA wall. The distribution quadrants of LSA orifices on MCA walls were compared among different groups. RESULTS Most orifices were located on the superior side of MCA firstly (46 of 95, 48.4%), followed by the dorsal side (22 of 95, 23.2%). In patients with LI, the visible numbers of ventral and inferior orifices on the ipsilateral side were significantly lower than healthy controls (p = 0.039 for ventral side, p = 0.002 for inferior side). Similarly, plaques occurred more frequently at the ventral (7 of 20, 35.0%) and the inferior (7 of 20, 35.0%) sides of MCA walls. CONCLUSIONS TOF-MRA at 7 T is capable of imaging orifices of LSA on MCA. In patients with LI, the decreased number of LSA orifices on the ventral and inferior sides corresponded with the distribution of MCA plaques. The results may indicate the vulnerability of LSA orifices in intracranial atherosclerosis, which was supposed to be the cause of LI in basal ganglia.

['Kong|Qingle|Q|', 'Zhang|Zihao|Z|', 'Yang|Qi|Q|', 'Fan|Zhaoyang|Z|', 'Wang|Bo|B|', 'An|Jing|J|', 'Zhuo|Yan|Y|']

2019

31189425

Nonconsensual Sharing of Private Sexually Explicit Media Among University Students.

This research was the first in the United Kingdom to examine the prevalence and nature of nonconsensual sharing of sexually explicit messages, pictures, and videos and to examine whether this varies according to gender and by role (i.e., perpetrator, victim, or as dual role of perpetrator/victim). In a sample of 391 young adults (aged 18-25 years), questionnaire data on subjective norms, consensual and nonconsensual sharing, and their motivations for these behaviors were collected. Perpetration of and victimization through nonconsensual sharing was experienced by a substantial number of individuals. There was an association between reporting perpetration of nonconsensual sharing and experiencing victimization. An association was also found between reporting being pressured (i.e., coerced) to send sexually explicit material and experiencing victimization of nonconsensual sharing, which suggests that these behaviors may form part of a continuum of violence and abuse, potentially within intimate relationships. No association was found between gender and (a) perpetration or (b) victimization. However, from a gendered perspective, females perceived there was greater social pressure to post messages, pictures, and videos, compared with males. Motivations for nonconsensual sharing were commonly explained as for fun/a joke, and generally not thought of as problematic, although some victims perceived motivations to be more negative and/or related to revenge/causing distress. Given that this research examined nonconsensual sharing across messages, pictures, and videos for both victimization and perpetration and found it was both perpetrated and experienced by females and males, this does not support the common perception that this is a male perpetrated behavior against women. This has implications for education, policy, intervention, and prevention, with approaches needing to be inclusive of both males and females when addressing perpetration and victimization.

['Walker|Kate|K|0000-0002-9796-2200', 'Sleath|Emma|E|', 'Hatcher|Ruth M|RM|', 'Hine|Benjamin|B|', 'Crookes|Rebecca L|RL|']

2021

34563444

Concurrent validity and precision of the thumb disability examination (TDX) in first carpometacarpal osteoarthritis.

STUDY DESIGN A descriptive psychometric study of precision and concurrent validity of the Thumb Disability Examination (TDX). INTRODUCTION Thumb carpometacarpal osteoarthritis (CMC OA) is a painful joint condition impacting the functionality of the hand. Therapists use patient-reported outcome measures to evaluate change in disability and symptomology in response to interventions. The TDX is the only condition-specific outcome measure for persons with thumb CMC OA. Its responsiveness, test-retest reliability and concurrent validity with the DASH are published, yet it's precision and concurrent validity with a hand-region-specific tool has not yet been established. PURPOSE OF THE STUDY We aimed to determine the precision and concurrent validity of the TDX with a region-specific outcome measure in people with thumb CMC OA. METHODS Sixteen individuals with a medical diagnosis of CMC OA or a positive pressure-shear test completed the TDX across two visits and the Brief Michigan Hand Questionnaire (bMHQ) at the initial visit. The second visit was 7 to 21 days after the first. Self-administration of the TDX and bMHQ were observed by a licensed occupational therapist. RESULTS Across total and subscale scores of the TDX, standard error of measurement (SEM) values are used to indicate the precision of tool and demonstrate how confident a user can be that change in score exceeds the error inherent to the tool. Minimal detectable change percentage (MDC%) values for the TDX are acceptable (<30%). The TDX demonstrated high concurrent validity with the bMHQ (rs = -0.733; P = .001). DISCUSSION Precision of the TDX is acceptable and the concurrent validity of the TDX with a commonly used region-specific scale is high. The study was limited by a small, demographically homogeneous sample due to difficulty in recruitment. CONCLUSIONS The TDX is a precise and valid outcome measure for individuals having a clinical diagnosis or indications of having thumb CMC OA.

['Johnson|Leah|L|', 'Karau|Ryan|R|', 'McGee|Corey|C|']

2021

32485201

Analysis of CCR3 expression in corneal neovascularization in a murine model and human corneas.

The aim of this study was to examine the expression of the cytokines and chemokines receptor-3 (CCR3) molecule in endothelial cells and vascular structures in a murine model of corneal neovascularization and in samples of neovascularized human corneas. An immunofluorescence assay using the murine model showed a greater proportion and intensity of CCR3 in the epithelium and corneal subepithelial regions in corneas with neovascularization. In the absence of vascularization, no CCR3 was found. Of the 32 studied tissues, eight were vascularized and 24 were avascular. In the human corneas, vascularized corneas showed positive labeling for CD31 in all the analzedtissues, as well as positive labeling for CCR3. Therefore, all vascularized tissues showed positive coexpression of CCR3 and CD31, whereas none of the avascular corneas showed immunolabeling for either of these receptors. These results suggest that CCR3 could be a possible marker for corneal neovascularization with potential to be a therapeutic target.

['Haber|Alberto|A|', 'Polania-Baron|Eduardo J|EJ|', 'Bolaños-Jimenez|Rodrigo|R|', 'Navas|Alejandro|A|', 'Graue Hernandez|Enrique O|EO|', 'Garfias|Yonathan|Y|']

2020

33636446

cAMP/PKA/CREB signaling pathway-mediated effects of melatonin receptor genes on clock gene expression in Bactrian camel ovarian granulosa cells.

The cAMP/PKA/CREB pathway is involved in the regulation of melatonin during important physiological activities in mammals. However, the regulation of circadian clock genes in ovarian granulosa cells remains unclear. Herein, we determined the relationship between melatonin and biological clock genes using cultured Bactrian camel ovarian granulosa cells. The enzyme-linked immunosorbent assays showed that the cAMP content was reduced when melatonin receptor (MT) genes or cryptochrome (Cry) genes were overexpressed; the quantitative polymerase chain reaction and western blot analyses revealed that the expression levels of all circadian clock genes (GNB2, PKA, CREB, Per1/2/3, and Clock) except Cry1/2 decreased significantly at 24 h. Cellular immunolocalization analysis showed that melatonin receptors were localized in the cell membrane and cytoplasm; the CRY protein was mainly localized in the nucleus. Overall, our findings indicated that the rhythmic regulation of ovarian granulosa cells was consistent with the regulatory action of the central circadian clock.

['Zhao|S-Q|SQ|', 'Gao|Y|Y|', 'Zhang|Y|Y|', 'Yang|X-P|XP|', 'Yang|Z|Z|']

2021

25529267

Present state of microchip electrophoresis: state of the art and routine applications.

Microchip electrophoresis (MCE) was one of the earliest applications of the micro-total analysis system (μ-TAS) concept, whose aim is to reduce analysis time and reagent and sample consumption while increasing throughput and portability by miniaturizing analytical laboratory procedures onto a microfluidic chip. More than two decades on, electrophoresis remains the most common separation technique used in microfluidic applications. MCE-based instruments have had some commercial success and have found application in many disciplines. This review will consider the present state of MCE including recent advances in technology and both novel and routine applications in the laboratory. We will also attempt to assess the impact of MCE in the scientific community and its prospects for the future.

['Castro|Eric R|ER|', 'Manz|Andreas|A|']

2015

29223883

Analysis of mainland Japanese and Okinawan Japanese populations using the precision ID Ancestry Panel.

We typed 165 AIMs in 49 mainland Japanese and 47 Okinawa Japanese using the Precision ID Ancestry Panel (Thermo Fisher Scientific). None of the 165 SNPs showed significant deviation from Hardy-Weinberg equilibrium in the mainland Japanese. One SNP (rs3943253) showed significant deviation from Hardy-Weinberg equilibrium in Okinawa Japanese. Fisher's exact tests showed that the genotype frequencies of 14 loci were significantly different (p<0.05) between the two populations before correction for multiple testing. After Bonferroni correction, only rs671 remained statistically significant (p<0.0003). This SNP is located in the ALDH2 gene. The mutant A allele is associated with increased side effects after alcohol intake. The frequency of the GG genotype (wild type) was higher in the Okinawa Japanese (78.7%) than in mainland Japanese (34.7%; Bonferroni corrected P<0.001). For 31 (63.3%) of the mainland Japanese and 42 (89.4%) of Okinawa Japanese, the highest population likelihood was obtained with the Japanese reference population. However, only in a few individuals, the likelihoods were significantly different from those calculated using reference data from neighboring populations. The likelihoods for mainland Japanese and Okinawa Japanese were not significantly different from each other for any of the investigated individuals. STRUCTURE and PCA analyses showed that mainland Japanese, Okinawa Japanese, and East Asians could not be differentiated with the Precision ID Ancestry Panel.

['Nakanishi|Hiroaki|H|', 'Pereira|Vania|V|', 'Børsting|Claus|C|', 'Yamamoto|Toshimichi|T|', 'Tvedebrink|Torben|T|', 'Hara|Masaaki|M|', 'Takada|Aya|A|', 'Saito|Kazuyuki|K|', 'Morling|Niels|N|']

2018

27835602

Inhibiting tumor necrosis factor-alpha diminishes desmoplasia and inflammation to overcome chemoresistance in pancreatic ductal adenocarcinoma.

BACKGROUND Pancreatic ductal adenocarcinoma (PDAC) is one of the most common cancer death reasons. Anti-tumor necrosis factor-alpha (TNF-α) antibodies have shown promising effects in PDAC pre-clinical models. However, the prognostic values of TNF-α, underlying mechanisms by which anti-TNF-α treatments inhibit PDAC, and potential synergistic effects of anti-TNF-α treatments with chemotherapy are still unclear. RESULTS AND METHODS To identify the targeting values of TNF-α in PDAC, we measured TNF-α expression in different stages of PDAC initiation and evaluated its prognostic significance in a pancreatic cancer cohort. We found that TNF-α expression elevated in PDAC initiation process, and high expression of TNF-α was an independent prognostic marker of poor survival. We further evaluated anti-tumor effects of anti-TNF-α treatments in PDAC. Anti-TNF-α treatments resulted in decreased cell viability in both PDAC tumor cells and pancreatic satellite cells in similar dose in vitro. In vivo, anti-TNF-α treatments showed effects in reducing desmoplasia and the tumor promoting inflammatory microenvironment in PDAC. Combination of anti-TNF-α treatments with chemotherapy partly overcame chemoresistance of PDAC tumor cells and prolonged the survival of PDAC mouse model. CONCLUSIONS In conclusion, our findings indicated that TNF-α in PDAC can be a prognostic and therapeutic target. Inhibition of TNF-α synergized with chemotherapy in PDAC resulted in better pre-clinical responses via killing tumor cells as well as diminishing desmoplasia and inflammation in PDAC tumor stroma.

['Zhao|Xianda|X|', 'Fan|Wei|W|', 'Xu|Zhigao|Z|', 'Chen|Honglei|H|', 'He|Yuyu|Y|', 'Yang|Gui|G|', 'Yang|Gang|G|', 'Hu|Hanning|H|', 'Tang|Shihui|S|', 'Wang|Ping|P|', 'Zhang|Zheng|Z|', 'Xu|Peipei|P|', 'Yu|Mingxia|M|']

2016

31670964

Band Filling and Cross Quantum Capacitance in Ion-Gated Semiconducting Transition Metal Dichalcogenide Monolayers.

Ionic liquid gated field-effect transistors (FETs) based on semiconducting transition metal dichalcogenides (TMDs) are used to study a rich variety of extremely interesting physical phenomena, but important aspects of how charge carriers are accumulated in these systems are not understood. We address these issues by means of a systematic experimental study of transport in monolayer MoSe2 and WSe2 as a function of magnetic field and gate voltage, exploring accumulated densities of carriers ranging from approximately 1014 cm-2 holes in the valence band to 4 × 1014 cm-2 electrons in the conduction band. We identify the conditions when the chemical potential enters different valleys in the monolayer band structure (the K and Q valley in the conduction band and the two spin-split K-valleys in the valence band) and find that an independent electron picture describes the occupation of states well. Unexpectedly, however, the experiments show very large changes in the device capacitance when multiple valleys are occupied that are not at all compatible with the commonly expected quantum capacitance contribution of these systems, CQ = e2/ (dμ/dn). A theoretical analysis of all terms responsible for the total capacitance shows that under general conditions a term is present besides the usual quantum capacitance, which becomes important for very small distances between the capacitor plates. This term, which we call cross quantum capacitance, originates from screening of the electric field generated by charges on one plate from charges sitting on the other plate. The effect is negligible in normal capacitors but large in ionic liquid FETs because of the atomic proximity between the ions in the gate and the accumulated charges on the TMD, and it accounts for all our experimental observations. Our findings therefore reveal an important contribution to the capacitance of physical systems that had been virtually entirely neglected until now.

['Zhang|Haijing|H|0000-0002-3305-2507', 'Berthod|Christophe|C|', 'Berger|Helmuth|H|', 'Giamarchi|Thierry|T|', 'Morpurgo|Alberto F|AF|0000-0003-0974-3620']

2019

34019251

Effect of Tris Buffer in the Intensity of the Multipoint Covalent Immobilization of Enzymes in Glyoxyl-Agarose Beads.

Tris is an extensively used buffer that presents a primary amine group on its structure. In the present work trypsin, chymotrypsin and penicillin G acylase (PGA) were immobilized/stabilized on glyoxyl agarose in presence of different concentrations of Tris (from 0 to 20 mM). The effects of the presence of Tris during immobilization were studied analyzing the thermal stability of the obtained immobilized biocatalysts. The results indicate a reduction of the enzyme stability when immobilized in the presence of Tris. This effect can be observed in inactivations carried out at pH 5, 7, and 9 with all the enzymes assayed. The reduction of enzyme stability increased with the Tris concentration. Another interesting result is that the stability reduction was more noticeable for immobilized PGA than in the other immobilized enzymes, the biocatalysts prepared in presence of 20 mM Tris lost totally the activity at pH 7 just after 1 h of inactivation, while the reference at this time still kept around 61 % of the residual activity. These differences are most likely due to the homogeneous distribution of the Lys groups in PGA compared to trypsin and chymotrypsin (where almost 50% of Lys group are in a small percentage of the protein surface). The results suggest that Tris could be affecting the multipoint covalent immobilization in two different ways, on one hand, reducing the number of available glyoxyl groups of the support during immobilization, and on the other hand, generating some steric hindrances that difficult the formation of covalent bonds.

['Braham|Sabrina Ait|SA|', 'Morellon-Sterling|Roberto|R|', 'de Andrades|Diandra|D|', 'Rodrigues|Rafael C|RC|', 'Siar|El-Hocine|EH|', 'Aksas|Ali|A|', 'Pedroche|Justo|J|', 'Millán|Maria Del Carmen|MDC|', 'Fernandez-Lafuente|Roberto|R|http://orcid.org/0000-0003-4976-7096']

2021

23781129

Comprehensive Evaluation of Biological Growth Control by Chlorine-Based Biocides in Power Plant Cooling Systems Using Tertiary Effluent.

Recent studies have shown that treated municipal wastewater can be a reliable cooling water alternative to fresh water. However, elevated nutrient concentration and microbial population in wastewater lead to aggressive biological proliferation in the cooling system. Three chlorine-based biocides were evaluated for the control of biological growth in cooling systems using tertiary treated wastewater as makeup, based on their biocidal efficiency and cost-effectiveness. Optimal chemical regimens for achieving successful biological growth control were elucidated based on batch-, bench-, and pilot-scale experiments. Biocide usage and biological activity in planktonic and sessile phases were carefully monitored to understand biological growth potential and biocidal efficiency of the three disinfectants in this particular environment. Water parameters, such as temperature, cycles of concentration, and ammonia concentration in recirculating water, critically affected the biocide performance in recirculating cooling systems. Bench-scale recirculating tests were shown to adequately predict the biocide residual required for a pilot-scale cooling system. Optimal residuals needed for proper biological growth control were 1, 2-3, and 0.5-1 mg/L as Cl2 for NaOCl, preformed NH2Cl, and ClO2, respectively. Pilot-scale tests also revealed that Legionella pneumophila was absent from these cooling systems when using the disinfectants evaluated in this study. Cost analysis showed that NaOCl is the most cost-effective for controlling biological growth in power plant recirculating cooling systems using tertiary-treated wastewater as makeup.

['Chien|Shih-Hsiang|SH|', 'Dzombak|David A|DA|', 'Vidic|Radisav D|RD|']

2013

34689908

Introduction to Apiculture (Apis mellifera).

Honey bees fulfill a critical role as the principal managed pollinator for modern agricultural ecosystems, necessary for the production of many of the world's food crops. The beekeeper must be a knowledgeable manager of bee health, apicultural production systems, and food safety practices. Veterinarians play a vital role in apiculture in supporting beekeepers to treat current and emerging diseases and pests.

['Pernal|Stephen F|SF|']

2021

28132598

The Narcissism Spectrum Model: A Synthetic View of Narcissistic Personality.

The narcissism spectrum model synthesizes extensive personality, social-psychological, and clinical evidence, building on existing knowledge about narcissistic grandiosity and vulnerability to reveal a view of narcissism that respects its clinical origins, embraces the diversity and complexity of its expression, and reflects extensive scientific evidence about the continuity between normal and abnormal personality expression. Critically, the proposed model addresses three key, inter-related problems that have plagued narcissism scholarship for more than a century. These problems can be summarized as follows: (a) What are the key features of narcissism? (b) How are they organized and related to each other? and (c) Why are they organized that way, that is, what accounts for their relationships? By conceptualizing narcissistic traits as manifested in transactional processes between individuals and their social environments, the model enables integration of existing theories of narcissism and thus provides a compelling perspective for future examination of narcissism and its developmental pathways.

['Krizan|Zlatan|Z|', 'Herlache|Anne D|AD|']

2018

24632348

Innate immunity in disease.

Cells can innately recognize generic products of viruses, bacteria, fungi, or injured tissue by engagement of pattern recognition receptors. Innate immune cells rapidly respond to this engagement to control commensals, thwart pathogens, and/or prompt repair. Insufficient or excessive activation of the innate immune response results in disease. This review focuses on pattern recognition receptors and cells of the innate immune system that are important for intestinal function. Our improving knowledge pertaining to this important aspect of our immune response is opening potential important new therapeutic opportunities for the treatment of disease.

['Elliott|David E|DE|', 'Siddique|Sana S|SS|', 'Weinstock|Joel V|JV|']

2014

28686122

Failure of ETV in patients with the highest ETV success scores.

OBJECTIVE Endoscopic third ventriculostomy (ETV) is a surgical alternative to placing a CSF shunt in certain patients with hydrocephalus. The ETV Success Score (ETVSS) is a reliable, simple method to estimate the success of the procedure by 6 months of postoperative follow-up. The highest score is 90, estimating a 90% chance of the ETV effectively treating hydrocephalus without requiring a shunt. Treatment with ETV fails in certain patients, despite their being the theoretically best candidates for the procedure. In this study the authors attempted to identify factors that further predicted success in patients with the highest ETVSSs. METHODS A retrospective review was performed of all patients treated with ETV at 3 institutions. Demographic, radiological, and clinical data were recorded. All patients by definition were older than 1 year, had obstructive hydrocephalus, and did not have a prior shunt. Failure of ETV was defined as the need for a shunt by 1 year. The ETV was considered a success if the patient did not require another surgery (either shunt placement or a repeat endoscopic procedure) by 1 year. A statistical analysis was performed to identify factors associated with success or failure. RESULTS Fifty-nine patients met the entry criteria for the study. Eleven patients (18.6%) required further surgery by 1 year. All of these patients received a shunt. The presenting symptom of lethargy statistically correlated with success (p = 0.0126, odds ratio [OR] = 0.072). The preoperative radiological finding of transependymal flow (p = 0.0375, OR 0.158) correlated with success. A postoperative larger maximum width of the third ventricle correlated with failure (p = 0.0265). CONCLUSIONS The preoperative findings of lethargy and transependymal flow statistically correlated with success. This suggests that the best candidates for ETV are those with a relatively acute elevation of intracranial pressure. Cases without these findings may represent the failures in this highly selected group.

['Gianaris|Thomas J|TJ|', 'Nazar|Ryan|R|', 'Middlebrook|Emily|E|', 'Gonda|David D|DD|', 'Jea|Andrew|A|', 'Fulkerson|Daniel H|DH|']

2017

28752042

Designing Cash Transfer Programs for an Older Population: The Mexican Case.

Aging populations and the prevalence of poverty in old age have led to the introduction of noncontributory pensions in many countries. We consider a number of alternative targeting approaches and simulate their effects in an empirical application in the State of Yucatan, Mexico. We compare the approaches with respect to leakage, under-coverage, and their effects on government budgets. We are also able to compare the simulated effects of one alternative with the observed effect of a recently introduced demogrant and find that the simulation is a close approximation of the empirical outcomes. We discuss issues of implementation and political feasibility.

['Aguila|Emma|E|', 'Kapteyn|Arie|A|', 'Tassot|Caroline|C|']

2017

29890502

Can grip strength be used as a surrogate marker to monitor recovery from shoulder fatigue?

Muscular fatigue impacts on normal shoulder function, which is particularly pertinent to throwing athletes. This study aimed to investigate the relationship between grip strength and shoulder muscle fatigue to evaluate the role of grip strength as a surrogate measure for upper limb performance. Twenty healthy participants were recruited. EMG was recorded from 15 shoulder muscles during different fatiguing contractions: an initial baseline recording (Fat-Baseline); after a shoulder exhausting exercise regime (Fat-Exhaustion); and after a 10 min rest period (Fat-Recovery). Grip strength was similarly measured in the same conditions. Grip strength differed significantly across the testing scenarios (p = 0.012-<0.001). Greater fatigue was seen in anterior deltoid, middle deltoid, posterior deltoid and supraspinatus in the Fat-Exhaustion contraction as compared to the Fat-Baseline contraction (p = <0.001-0.043). Greater fatigue was seen during the Fat-Recovery contraction for the trapezius, serratus anterior and biceps brachii as compared to the Fat-Exhaustion contraction (p = 0.008-0.038). Grip strength decreased following an exhausting exercise protocol but recovered to baseline following a rest period. Conversely, EMG indices of fatigue did not recover. Additional fatigue was seen reflecting a reorganisation of movement strategy. Therefore, susceptibility to injury still exists if grip strength alone is used as a barometer of upper limb performance.

['Hawkes|David|D|', 'Grant|Michael|M|', 'McMahon|Jessica|J|', 'Horsley|Ian|I|', 'Khaiyat|Omid|O|']

2018

27686069

Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus.

BACKGROUND In eukaryotic organisms, Piwi-interacting RNAs (piRNAs) control the activities of mobile genetic elements and ensure genome maintenance. Recent evidence indicates that piRNAs are involved in multiple biological pathways, including transcriptional regulation of protein-coding genes, sex determination and even interactions between host and pathogens. Aedes albopictus is a major invasive species that transmits a number of viral diseases in humans. Ae. albopictus has the largest genome and the highest abundance of repetitive sequences when compared with members that belong to Culicidae with a published genome. Analysis of piRNA profiles will provide a developmental and evolutionary perspective on piRNAs in Ae. albopictus. METHODS piRNAs were identified and characterized during the development of Ae. albopictus, and piRNA expression patterns in adult males and females as well as sugar-fed females and blood-fed females were compared. RESULTS Our results reveal that, despite the large genome size of Ae. albopictus, the piRNA pool of Ae. albopictus (1.2 × 107) is smaller than those of Aedes aegypti (1.7 × 107) and Drosophila melanogaster (1.6 × 107). In Ae. albopictus, piRNAs displayed the highest abundance at the embryo stage and the lowest abundance at the pupal stage. Approximately 50 % of the piRNAs mapped to intergenic regions with no known functions. Approximately 30 % of the piRNAs mapped to repetitive elements, and 77.69 % of these repeat-derived piRNAs mapped to Class I TEs; 45.42 % of the observed piRNA reads originated from piRNA clusters, and most of the top 10 highest expressed piRNA clusters and 100 highest expressed piRNAs from each stage displayed biased expression patterns across the developmental stages. All anti-sense-derived piRNAs displayed a preference for uridine at the 5' end; however, the sense-derived piRNAs showed adenine bias at the tenth nucleotide position and a typical ping-pong signature, suggesting that the biogenesis of piRNAs was conserved throughout development. Our results also show that 962 piRNAs displayed sex-biased expression, and 522 piRNAs showed higher expression in the blood-fed females than in the sugar-fed females. CONCLUSIONS Our results suggest that piRNAs, aside from silencing transposable elements in Ae. albopictus, may have a role in other biological pathways.

['Liu|Peiwen|P|', 'Dong|Yunqiao|Y|', 'Gu|Jinbao|J|', 'Puthiyakunnon|Santhosh|S|', 'Wu|Yang|Y|', 'Chen|Xiao-Guang|XG|']

2016

31686304

Multiscale composite model of fiber-reinforced tissues with direct representation of sub-tissue properties.

In many fiber-reinforced tissues, collagen fibers are embedded within a glycosaminoglycan-rich extrafibrillar matrix. Knowledge of the structure-function relationship between the sub-tissue properties and bulk tissue mechanics is important for understanding tissue failure mechanics and developing biological repair strategies. Difficulties in directly measuring sub-tissue properties led to a growing interest in employing finite element modeling approaches. However, most models are homogeneous and are therefore not sufficient for investigating multiscale tissue mechanics, such as stress distributions between sub-tissue structures. To address this limitation, we developed a structure-based model informed by the native annulus fibrosus structure, where fibers and the matrix were described as distinct materials occupying separate volumes. A multiscale framework was applied such that the model was calibrated at the sub-tissue scale using single-lamellar uniaxial mechanical test data, while validated at the bulk scale by predicting tissue multiaxial mechanics for uniaxial tension, biaxial tension, and simple shear (13 cases). Structure-based model validation results were compared to experimental observations and homogeneous models. While homogeneous models only accurately predicted bulk tissue mechanics for one case, structure-based models accurately predicted bulk tissue mechanics for 12 of 13 cases, demonstrating accuracy and robustness. Additionally, six of eight structure-based model parameters were directly linked to tissue physical properties, further broadening its future applicability. In conclusion, the structure-based model provides a powerful multiscale modeling approach for simultaneously investigating the structure-function relationship at the sub-tissue and bulk tissue scale, which is important for studying multiscale tissue mechanics with degeneration, disease, or injury.

['Zhou|Minhao|M|', 'Bezci|Semih E|SE|', "O'Connell|Grace D|GD|http://orcid.org/0000-0003-0746-1638"]

2020

34674154

Anatomy of Being, Metaphysics of Death: The Case of Avicenna's Logical Dissection.

Elucidating a metaphysics of medicine is vital for framing a coherent medical ethics. In this paper, I examine the historical case of Avicenna, the eleventh century physician-philosopher. Avicenna radicalizes the dissective power of reason using a logicized Aristotelian metaphysics to clarify concepts at the metaphysical level, which I call his anatomy of being. One of the practical consequences of Avicenna's metaphysics is a dehumanizing eschatology of death. I outline the main elements of Avicenna's thought that constitute his anatomy of being. Through an examination of his logic, metaphysics, and psychology, I show how Avicenna develops a dissective logic. I conclude that one's epistemology, as a method of knowing, entails a metaphysics, and, in turn, results in an ethical stance to the object of knowledge. For Avicenna, mental dissective logic applied to humans results in dehumanization, thereby destroying the humanistic impulse of medicine.

['Kornu|Kimbell|K|http://orcid.org/0000-0002-1583-8223']

2021

30686977

Prolonged Periods of Social Isolation From Weaning Reduce the Anti-inflammatory Cytokine IL-10 in Blood and Brain.

Life stressors during critical periods are reported to trigger an immune dysfunction characterised by abnormal production of inflammatory cytokines. Despite the relationship between early stressors and schizophrenia is described, the evidence on inflammatory biomarkers remains limited. We aimed to investigate whether an imbalance between pro- and anti-inflammatory cytokines in the brain is reflected in the peripheral blood of rats submitted to post-weaning social isolation (pwSI), a model with validity to study schizophrenia. We evaluated pro- and anti-inflammatory cytokines (IL-6, TNF-α, and IL-10) simultaneously at blood, prefrontal cortex and hippocampal tissues (Milliplex MAP), including the respective cytokines gene expression (mRNA) (qRT-PCR TaqMan mastermix). We also performed a correlation matrix to explore significant correlations among cytokines (protein and mRNA) in blood and brain, as well as cytokines and total number of square crossings in the open field for isolated-reared animals. Male Wistar rats (n = 10/group) were kept isolated (n = 1/cage) or grouped (n = 3-4/cage) since weaning for 10 weeks. After this period, rats were assessed for locomotion and sacrificed for blood and brain cytokines measurements. Prolonged pwSI decreased IL-10 protein and mRNA in the blood, and IL-10 protein in the hippocampus, along with decreased IL-6 and its mRNA expression in the prefrontal cortex. Our results also showed that cytokines tend to correlate to one-another among the compartments investigated, although blood and brain correlations are far from perfect. IL-10 hippocampal levels were negatively correlated with hyperlocomotion in the open field. Despite the unexpected decrease in IL-6 and unchanged TNF-α levels contrast to the expected pro-inflammatory phenotype, this may suggest that reduced anti-inflammatory signalling may be critical for eliciting abnormal behaviour in adulthood. Altogether, these results suggest that prolonged early-life adverse events reduce the ability to build proper anti-inflammatory cytokine that is translated from blood-to-brain.

['Corsi-Zuelli|Fabiana|F|', 'Fachim|Helene Aparecida|HA|', 'Loureiro|Camila Marcelino|CM|', 'Shuhama|Rosana|R|', 'Bertozi|Giuliana|G|', 'Joca|Sâmia Regiane Lourenço|SRL|', 'Menezes|Paulo Rossi|PR|', 'Louzada-Junior|Paulo|P|', 'Del-Ben|Cristina Marta|CM|']

2018

27198698

Antenatal diagnosis of idiopathic dilatation of pulmonary artery with 3D power doppler imaging.

We present a case of antenatal diagnosis of idiopathic dilatation of the pulmonary artery by three-dimensional power Doppler imaging (HDliveFlow with silhouette mode). Two-dimensional sonography clearly demonstrated a 10.4-mm-diameter fetal pulmonary artery (PA), whereas the aorta (Ao) diameter was 5.04 mm. HDliveFlow clearly demonstrated the spatial relationships and different sizes of PA and Ao. The PA size returned to normal (8.0 mm) on the 11th day after birth (Ao: 9.0 mm). HDliveFlow may be an adjunctive tool to two-dimensional sonography to diagnose abnormalities of fetal great vessels. © 2016 Wiley Periodicals, Inc. J Clin Ultrasound 45:121-123, 2017.

['Yang|Pei-Yin|PY|', 'Sajapala|Suraphan|S|', 'Yamamoto|Kenta|K|', 'Mori|Nobuhiro|N|', 'Kanenishi|Kenji|K|', 'Koyano|Kosuke|K|', 'Kusaka|Takashi|T|', 'Hata|Toshiyuki|T|']

2017

32508904

Factors Affecting the Degree of Angular Remodeling in Stent-Assisted Coiling of Bifurcation Aneurysms.

Background Stent-assisted coiling (SAC) leads to significant changes in the vascular angle altering the bifurcation geometry and the hemodynamics at the bifurcation apex. It is believed that the stent alone exerts this effect, but other possible factors have not been studied. Purpose To study the factors contributing to angular remodeling following SAC of bifurcation aneurysms including the anatomical, stent-related, and coil-related factors. Materials and Methods We reviewed 43 basilar and carotid bifurcation aneurysms treated by SAC using Neuroform EZ (n = 28), Enterprise (n = 8), and Lvis Jr. (n = 7) stents. The bifurcation angle between the mother and stented daughter vessel was measured in 4 settings: pretreatment, poststenting, postcoiling, and at delayed follow-up (6-12 months). The degree of stent-induced angular remodeling was calculated by subtracting the poststenting bifurcation angle from the pretreatment one, while the coil-induced angular remodeling was calculated as the difference between the postcoiling and poststenting bifurcation angle. The immediate postprocedural degree of angular remodeling is the sum of the stent- and coil-induced angular remodeling. We studied the effect of several factors including the pretreatment bifurcation angle, aneurysm site, diameter of parent vessel, stent type, stent length in the daughter vessel, postoperative actual in situ coil size, and packing density. Results The mean degree of stent-induced and coil-induced angular remodeling was 10.2 (0-47) and 4.53 (-7 to 30), respectively. The immediate postprocedural and delayed angular remodeling was 14.8 (-4 to 47) and 4.75 (-12 to 40), respectively. The degree of immediate remodeling was significantly affected by the actual in situ coil size (p = 0.017), and the pretreatment bifurcation angle (p = 0.024). Linear regression was carried out and the pretreatment bifurcation angle was defined as a predictor. The degree of delayed remodeling is significantly affected only by the pretreatment bifurcation angle (p = 0.011). Conclusion Immediate angular remodeling following SAC of bifurcation aneurysms can happen after stenting or coiling or both. This is the first study reporting the role of the coils as an additional factor beside the stent in inducing immediate angular changes; this effect is correlated to the size of the coils. The pretreatment bifurcation angle is the most consistent factor affecting the degree of both immediate and delayed angular remodeling.

['Saied|Ahmed|A|', 'Elsaid|Nada|N|', 'Joshi|Krishna|K|', 'Gomaa|Mohamed|M|', 'Amer|Talal|T|', 'Saad|Mohamed|M|', 'Lopes|Demetrius|D|']

2020

26170919

Differential expression and therapeutic efficacy of microRNA-346 in diabetic nephropathy mice.

Diabetic nephropathy (DN) is a major cause of end-stage renal disease, in which the SMAD signaling pathway plays an important role. The aim of the present study was to identify differentially expressed microRNAs (miRNAs) during the progression of DN and to investigate a selected miRNA in relation to SMAD3/4 and its therapeutic efficacy. The miRNA microarray was used to identify differentially expressed miRNAs in db/db DN mice. Reverse transcription-quantitative polymerase chain reaction and immunoblot analyses were used to detect SMAD3/4 expression. The development of DN in the db/db mice was demonstrated by glucose dysregulation and typical morphological changes in the kidney. miRNA-346 (miR-346) was identified as one of the differentially expressed miRNAs. The expression of SMAD3/4 was significantly attenuated by miR-346 administration and the therapeutic effects of miR-346 were observed in the DN mouse models. miR-346 was identified as a negative regulator of SMAD3/4. SMAD3/4 was upregulated in the renal tissue of db/db mice. The administration of miR-346 attenuated the SMAD3/4 expression in renal tissue and ameliorated the renal function and glomerular histology in DN mice. This study paves the way for clinical studies of miR-346 in DN.

['Zhang|Yong|Y|', 'Xiao|Hou-Qin|HQ|', 'Wang|Yang|Y|', 'Yang|Zhuo-Shun|ZS|', 'Dai|Long-Jun|LJ|', 'Xu|Yan-Cheng|YC|']

2015

34826118

Aldosterone and renin concentrations were abnormally elevated in a cohort of normotensive pregnant women.

BACKGROUND During pregnancy, the renin-angiotensin-aldosterone system (RAAS) undergoes major changes to preserve normal blood pressure (BP) and placental blood flow and to ensure a good pregnancy outcome. Abnormal aldosterone-renin metabolism is a risk factor for arterial hypertension and cardiovascular risk, but its association with pathological conditions in pregnancy remains unknown. Moreover, potential biomarkers associated with these pathological conditions should be identified. AIM To study a cohort of normotensive pregnant women according to their serum aldosterone and plasma renin levels and assay their small extracellular vesicles (sEVs) and a specific protein cargo (LCN2, AT1R). METHODS A cohort of 54 normotensive pregnant women at term gestation was included. We determined the BP, serum aldosterone, and plasma renin concentrations. In a subgroup, we isolated their plasma sEVs and semiquantitated two EV proteins (AT1R and LCN2). RESULTS We set a normal range of aldosterone and renin based on the interquartile range. We identified 5/54 (9%) pregnant women with elevated aldosterone and low renin levels and 5/54 (9%) other pregnant women with low aldosterone and elevated renin levels. No differences were found in sEV-LCN2 or sEV-AT1R. CONCLUSION We found that 18% of normotensive pregnant women had either high aldosterone or high renin levels, suggesting a subclinical status similar to primary aldosteronism or hyperreninemia, respectively. Both could evolve to pathological conditions by affecting the maternal vascular and renal physiology and further the BP. sEVs and their specific cargo should be further studied to clarify their role as potential biomarkers of RAAS alterations in pregnant women.

['Pastén|Valentina|V|', 'Tapia-Castillo|Alejandra|A|', 'Fardella|Carlos E|CE|', 'Leiva|Andrea|A|', 'Carvajal|Cristian A|CA|http://orcid.org/0000-0002-0668-412X']

2021

33642471

Impact of COVID-19 Outbreak on the Long-Range Transport of Common Air Pollutants in KUWAMS.

As a background sampling site in western Japan, the Kanazawa University Wajima Air Monitoring Station (KUWAMS) continuously observes the air pollutants, including PM1, PM2.5, organic carbon (OC) and element carbon (EC). Data for September 2019 to April 2020 were compared with data for September 2018 to April 2019. The mean concentrations of both PM1 and PM2.5 were 4.10 µg/m3 (47%) and 5.82 µg/m3 (33%) lower, respectively in the Coronavirus Disease 2019 (COVID-19) period (January to April) than in the same period in 2019. Notably, the average concentrations of both classes of particulate matter (PM) in the COVID-19 period were the lowest for that period in all years since 2016. OC and EC also considerably lower (by 69 and 63%, respectively) during the COVID-19 period than during the same period in 2019. All pollutants were then started to increase after the resumption of the work in 2020. The pollutant variations correspond to the measure implemented during the COVID-19 period, including the nationwide lockdown and work resumption. Furthermore, the reductions in the ratios PM1/PM2.5 and OC/EC during COVID-19 period indicate lighter pollution and fewer emission sources. This analysis of the changes in the pollutant concentrations during the epidemic and non-epidemic periods illustrates the significance of the dominant pollution emissions at KUWAMS and the impact of pollution from China that undergoes long-range transport to KUWAMS.

['Zhang|Hao|H|', 'Zhang|Lulu|L|', 'Yang|Lu|L|', 'Zhou|Quanyu|Q|', 'Zhang|Xuan|X|', 'Xing|Wanli|W|', 'Hayakawa|Kazuichi|K|', 'Toriba|Akira|A|', 'Tang|Ning|N|']

2021

34150136

Dynamics of heart rate variability in patients with type 2 diabetes mellitus during spinal anesthesia using dexmedetomidine.

Objective The aim of this study was to investigate the heart rate variability (HRV) in patients with Type 2 diabetes mellitus (T2DM) who underwent spinal anesthesia using dexmedetomidine for lower limb surgery. METHODS T2DM patients were divided into two groups, namely the controlled group (HbA1c < 7%) and the uncontrolled group (HbA1c > 7%) according to the glycosylated hemoglobin (HbA1c) level, and patients with non-T2DM as the normal group, 30 cases in each group. The HRV, including low-frequency (LF) power, high-frequency (HF) power, total power (TP) and LF/HF ratio, was measured 10 min before spinal anesthesia (T0) and 10 min (T1), 20 min (T2) and 30 min (T3) after spinal anesthesia with dexmedetomidine. RESULTS We observed that TP, LF, and HF power in the uncontrolled group were remarkably lower than that in the other two groups at T0 (P < 0.05). In the controlled group, the LF power dropped markedly at T1-2 than the normal group. The LF power in the uncontrolled group did not show significant change at all time points, but was significantly lower than the level in the controlled group at T1-3. The HF power in the three groups did not alter markedly at different time points, but the HF power in the uncontrolled group was markedly lower than that in the normal group and the controlled group. In all three groups, the LF/HF ratio dropped markedly at T1-3 with no markedly difference between the groups. The heart rhythms in the three groups showed a decrease trend after spinal anesthesia with no markedly difference between the groups. The SBP and DBP at T1-3 in the three groups were markedly lower than that at T0, and the systolic blood pressure (SBP) and diastolic blood pressure (DBP) at T1-3 in the uncontrolled group were markedly higher than those in the normal group and the controlled group. CONCLUSION Spinal anesthesia with dexmedetomidine affects autonomic nerve function in patients whose glycemic control is better during the lower limb surgery in T2DM patients, but has no significant effect on patients who fail to do so. For such patients, spinal anesthesia can result in a markedly increase in SBP and DBP.

['Ye|Liying|L|', 'Dai|Bo|B|', 'Wu|Zheyou|Z|', 'Hu|Ying|Y|']

2021

34180737

An Automatic Biopsy Needle Detection and Segmentation on Ultrasound Images Using a Convolutional Neural Network.

Needle visualization in the ultrasound image is essential to successfully perform the ultrasound-guided core needle biopsy. Automatic needle detection can significantly reduce the procedure time, false-negative rate, and highly improve the diagnosis. In this paper, we present a CNN-based, fully automatic method for detection of core needle in 2D ultrasound images. Adaptive moment estimation optimizer is proposed as CNN architecture. Radon transform is applied to locate the needle. The network's model was trained and tested on the total of 619 2D images from 91 cases of breast cancer. The model has achieved an average weighted intersection over union (the weighted Jaccard Index) of 0.986, F1 Score of 0.768, and angle RMSE of 3.73°. The obtained results exceed the other solutions by at least 0.27 and 7° in case of F1 score and angle RMSE, respectively. Finally, the needle is detected in a single frame averagely in 21.6 ms on a modern PC.

['Wijata|Agata|A|', 'Andrzejewski|Jacek|J|', 'Pyciński|Bartłomiej|B|0000-0002-6634-4127']

2021

32847825

Implementation of health and health-related sustainable development goals: progress, challenges and opportunities - a systematic literature review.

INTRODUCTION While health is one of the Sustainable Development Goals (SDGs), many other 'health-related' goals comprise determinants of health. Integrated implementation across SDGs is needed for the achievement of Agenda 2030. While existing literature is rich in normative recommendations about potentially useful approaches, evidence of implementation strategies being adopted by countries is limited. METHODS We conducted a systematic review with qualitative synthesis of findings using peer reviewed and grey literature from key databases. We included publications examining implementation of health and health-related SDGs (HHSDGs) at national or subnational level published between June 2013 and July 2019. RESULTS Of the 32 included publications, 24 provided information at the national level while eight provided information for multiple countries or regions. Our findings indicate that high-level political commitment is evident in most countries and HHSDGs are being aligned with existing national development strategies and plans. A multisectoral, integrated approach is being adopted in institutional setups but evidence on effectiveness of these approaches is limited. Funding constraints are a major challenge for many countries. HHSDGs are generally being financed from within existing funded plans and, in some instances, through SDG-specific budgeting and tracking; additional funding is being mobilised by increasing domestic taxation and subsidisation, and by collaborating with development partners and private sector. Equity is being promoted by improving health service access through universal health coverage and social insurance schemes, especially for disadvantaged populations. Governments are collaborating with development partners and UN agencies for support in planning, institutional development and capacity building. However, evidence on equity promotion, capacity building initiatives and implementation approaches at subnational level is limited. Lack of coordination among various levels of government emerges as a key challenge. CONCLUSION strengthening implementation of multisectoral work, capacity building, financial sustainability and data availability are key considerations to accelerate implementation of HHSDGs.

['Aftab|Wafa|W|0000-0001-5040-5215', 'Siddiqui|Fahad Javaid|FJ|0000-0002-9046-5105', 'Tasic|Hana|H|', 'Perveen|Shagufta|S|', 'Siddiqi|Sameen|S|0000-0001-8289-0964', 'Bhutta|Zulfiqar Ahmed|ZA|0000-0003-0637-599X']

2020

30117068

Automatic directional analysis of cell fluorescence images and morphological modeling of microfilaments.

Cytoskeleton and nucleus are two important anatomic components in eukaryotic cells. Cell fluorescence images are employed to study their realignment and deformation during cell extrusion. Quantitative analysis and modeling of cell orientation are investigated in this paper. For orientation measurement, alignment orientation of microfilaments is calculated using structure tensor method. Nuclei is segmented and fitted to ellipses in nuclei images. Based on the fitted ellipse, orientation and aspect ratio of each nucleus are computed. A morphological model is proposed to describe the movement of microfilaments quantitatively. The parameters of the model are determined by in-plane stresses obtained by numerical simulation. The proposed automatic orientation measurement algorithms can help to analyze the relationship between cell orientation and stress qualitatively. The proposed morphological model is the first model to quantitatively describe the relationship of microfilament movement with stress. Experimental results show that cell and nucleus tend to align along in-plane maximum shear stress and the proposed morphological model is a reasonable model for cell movement. The modeling of cell behavior under different stress can facilitate biomedical research such as tissue engineering and cancer analysis. Graphical abstract ᅟ.

['Zhou|Yue|Y|', 'Li|Huiqi|H|http://orcid.org/0000-0002-8720-3374', 'Zhang|Wanjun|W|', 'Xu|Jiayi|J|', 'Li|Xiaojun|X|', 'Ji|Baohua|B|']

2019

29209855

Osteoporotic hip fractures in Black South Africans: a regional study.

Osteoporotic hip fractures are thought to be rare in Blacks however, this study from South Africa shows a significant increase in the number of hip fracture in Blacks. With the expected increase in older people, osteoporotic fractures will pose a major health problem and screening guidelines needed to be implemented. INTRODUCTION Developing countries are predicted to bear the burden of osteoporosis in the coming decades. This study was undertaken to review earlier reports that osteoporotic hip fractures are rare in Black Africans. METHODS In an observational study, the incidence rates and relative risk ratios (RRR) of osteoporotic hip fractures were calculated in the Black population, aged 60 years and older, residing in the eThekwini region of South Africa. All Black subjects, presenting with a minimal trauma hip fracture to five public hospitals in the region, entered the study. Descriptive statistics were applied to show differences in age and sex. RESULTS Eighty-seven subjects were enrolled in the study with a mean age of 76.5 ± 10.5 years and the sex ratio of women to men was 2.5:1. Although men were younger than women, this was not significant (74.2 ± 12.3 vs. 77.4 ± 9.6 years, p = 0.189). The age-adjusted rate was 69.2 per 100,000 p.a. for women and 73.1 per 100,000 p.a. for men. There was a significant increase in the relative risk ratios for hip fractures after the age of 75 years in the total cohort and in women and men. Except for the 65-69-year age group, there was no significant difference in the age-adjusted RRR between women and men. CONCLUSION This study represents the largest number of hip fractures recorded in Black Africans. Although the incidence rate is approximately tenfold higher than previously recorded, it remains amongst the lowest globally. A national registry inclusive of private and public sector is required to establish the true incidence rate of hip fractures in Black Africans.

['Paruk|F|F|', 'Matthews|G|G|', 'Cassim|B|B|']

2017

26616052

Effects of vimentin disruption on the mechanoresponses of articular chondrocyte.

Human articular cartilage is subjected to repetitive mechanical loading during life time. As the only cellular component of articular cartilage, chondrocytes play a key role in the mechanotransduction within this tissue. The mechanoresponses of chondrocytes are largely determined by the cytoskeleton. Vimentin intermediate filaments, one of the major cytoskeletal components, have been shown to regulate chondrocyte phenotype. However, the contribution of vimentin in chondrocyte mechanoresponses remains less studied. In this study, we seeded goat articular chondrocytes on a soft polyacrylamide gel, and disrupted the vimentin cytoskeleton using acrylamide. Then we applied a transient stretch or compression to the cells, and measured the changes of cellular stiffness and traction forces using Optical Magnetic Twisting Cytometry and Traction Force Microscopy, respectively. In addition, to study the effects of vimentin disruption on the intracellular force generation, we treated the cells with a variety of reagents that are known to increase or decrease cytoskeletal tension. We found that, after a compression, the contractile moment and cellular stiffness were not affected in untreated chondrocytes, but were decreased in vimentin-disrupted chondrocytes; after a stretch, vimentin-disrupted chondrocytes showed a lower level of fluidization-resolidification response compared to untreated cells. Moreover, vimentin-disrupted chondrocytes didn't show much difference to control cells in responding to reagents that target actin and ROCK pathway, but showed a weaker response to histamine and isoproterenol. These findings confirmed chondrocyte vimentin as a major contributor in withstanding compressive loading, and its minor role in regulating cytoskeletal tension.

['Chen|Cheng|C|', 'Yin|Li|L|', 'Song|Xiongbo|X|', 'Yang|Hao|H|', 'Ren|Xiang|X|', 'Gong|Xiaoyuan|X|', 'Wang|Fuyou|F|', 'Yang|Liu|L|']

2016

33366291

The complete chloroplast genome of Houttuynia cordata Thunb. (Family: Saururaceae).

The chloroplast genome sequence of Houttuynia cordata has been characterized from Illumina pair-end sequencing. The complete chloroplast genome was 161,090 bp in length, containing a large single-copy (LSC) region of 88,180 bp and a small single-copy (SSC) region of 19,204 bp, which were separated by a pair of 26,853 bp inverted repeat regions. Furthermore, phylogenetic analysis revealed that H. cordata is a sister of Piper cenocladum.

['Yu|Feng|F|', 'Liu|Ya|Y|', 'Zhang|Renyi|R|https://orcid.org/0000-0002-3647-1416']

2019

29273032

Self-sampling for human papillomavirus DNA detection: a preliminary study of compliance and feasibility in BOLIVIA.

BACKGROUND Cervical cancer incidence and mortality rates in Bolivia are among the highest in Latin America. This investigation aims to evaluate the possibility of using simple devices, e.g. a cotton swab and a glass slide, for self-sampling in order to detect human papillomavirus (HPV) DNA by PCR in cervico-vaginal cells. METHODS In the first phase of our study we evaluated the use of a glass slide as a transport medium for cervical cells. A physician took paired-cervical samples from 235 women. One sample was transported in Easyfix® solution and the other sample was smeared over a glass slide. Both were further analyzed and compared for human DNA recovery and HPV detection. A kappa value was determined to evaluate the agreement between the HPV DNA detection rates. In the second phase of the study, 222 women from the urban, peri-urban and rural regions of Cochabamba were requested to perform self-sampling using the following devices: a cotton swab combined with a glass slide, and a vaginal tampon. Women gave their opinion about the self-sampling technique. Finally, the agreement for high risk-HPV detection between self- and physician-collected samples was performed in 201 samples in order to evaluate the self-sampling technique. RESULTS Firstly, the comparison between Easyfix® solution and the glass slide to transport clinical samples gave a good agreement for HPV DNA detection (κ = 0.71, 95% CI 0.60-0.81). Secondly, self-sampling, especially with cotton swab combined with glass slide, would generally be preferred over clinician sampling for a screening program based on HPV detection. Finally, we showed a good agreement between self- and physician collected samples for high risk-HPV detection (κ = 0.71, 95% CI 0.55-0.88). CONCLUSIONS Simple devices such as a cotton swab and a glass slide can be used to perform self-sampling and HPV DNA detection. Furthermore, most Bolivian women preferred self-sampling over clinician-sampling for cervical cancer screening.

['Surriabre|Pedro|P|', 'Allende|Gustavo|G|', 'Prado|Marcela|M|', 'Cáceres|Leyddy|L|', 'Bellot|Diego|D|', 'Torrico|Andrea|A|', 'Ustariz|Karina|K|', 'Rojas|Shirley|S|', 'Barriga|Jaime|J|', 'Calle|Pamela|P|', 'Villarroel|Ligia|L|', 'Yañez|Rosse Mary|RM|', 'Baay|Marc|M|', 'Rodriguez|Patricia|P|', 'Fontaine|Véronique|V|0000-0001-7588-5462']

2017

26429336

Age- and gender-dependent myocardial transcription patterns of cytokines and extracellular matrix remodelling enzymes in cats with non-cardiac diseases.

BACKGROUND Age, gender and systemic diseases all influence cardiac function and remodelling. In cats, age and gender associated myocardial remodelling and the effect of systemic diseases on the myocardium have so far not been studied. The aim of the study was therefore to investigate whether relevant cytokines and extracellular matrix (ECM) remodelling enzymes are expressed in the myocardium of cats with non-cardiac diseases and whether transcription levels are influenced by age and gender. METHODS The study was performed on myocardial samples from 26 cats aged between 2 and 19 years that had died with non-cardiac diseases. Seventeen cats were female (2 entire) and nine were male (1 entire). Of these, nine cats were diagnosed with diseases unlikely to affect the myocardium (control cats). The remaining 17 cats suffered from diseases with likely systemic effects. All hearts were assessed for any pathological changes, and the myocardium was analysed for interleukin (IL)-1, -2, -4, -6, -18, tumour necrosis factor (TNF)-α, interferon (IFN)-γ, transforming growth factor (TGF)-β, matrix metalloproteinase (MMP)-2, -3, -13, tissue inhibitor of MMP (TIMP)-1, -2 and -3 transcriptions using quantitative RT-PCR assays. RESULTS Despite the absence of any histological evidence of myocardial damage, inflammation and fibrosis, the myocardium of all the cats was found to constitutively transcribe cytokines and ECM remodelling enzymes, with generally higher mRNA concentrations in the atria than in the ventricles. The young and male cats exhibited higher transcription levels throughout the myocardium in comparison to the older and female cats. Furthermore, age-associated transcription pattern differed between male and female cats. CONCLUSION The constitutive transcription of ECM remodelling enzymes suggests continuous myocardial remodelling throughout the entire life of a cat. The myocardium of young and male cats appears to be in a pro-inflammatory state, whereas in older and female cats the myocardium exhibits a reduced inflammatory reaction to systemic disease. Age-associated cardiac remodelling seems to be influenced by non-hormonal factors in male and female cats.

['Fonfara|Sonja|S|', 'Hetzel|Udo|U|', 'Hahn|Shelley|S|', 'Kipar|Anja|A|']

2015

34392117

Nanocomplexes of curcumin and glycated bovine serum albumin: The formation mechanism and effect of glycation on their physicochemical properties.

Bovine serum albumin (BSA) and BSA-glucose conjugates (GBSAⅠ and GBSAⅠI) with different extent of glycation were complexed with curcumin (CUR). The formation mechanism of BSA/GBSA-CUR complexes and the effect of glycation on their physicochemical properties were investigated. Fluorescence quenching and FTIR analysis indicated that the BSA/GBSA-CUR nanocomplexes were formed mainly by hydrophobic interactions. XRD analysis demonstrated that CUR was present in an amorphous state in the nanocomplexes. BSA with a greater extent of glycation (BSA < GBSAⅠ<GBSAⅠI) displayed a higher binding affinity for CUR. The highest CUR encapsulation efficiency (86.77%) and loading capacity (7.81 mg/g) were obtained in the GBSAⅠI-CUR nanocomplex. The zeta-potential varied from -17.45 to -27.65 mV, depending on the extent of glycation. Furthermore, the physicochemical stability of BSA/GBSA-CUR nanocomplexes increased with the increasing extent of glycation of BSA. Thus, the obtained GBSAⅠI have the potential to become new delivery carriers for encapsulating hydrophobic food components.

['Fu|Jing-Jing|JJ|', 'Sun|Cong|C|', 'Tan|Zhi-Feng|ZF|', 'Zhang|Guang-Yao|GY|', 'Chen|Gui-Bing|GB|', 'Song|Liang|L|']

2022

30172573

Training physicians in behavioural change counseling: A systematic review.

BACKGROUND Poor health behaviours (e.g., smoking, physical inactivity) represent major underlying causes of non-communicable chronic diseases (NCDs). Prescriptive behaviour change interventions employed by physicians show limited effectiveness. Physician training in evidence-based behaviour change counselling (BCC) may improve behavioural risk factor management, but the efficacy and feasibility of current programs remains unclear. OBJECTIVE (1) To systematically review the efficacy of BCC training programs for physicians, and (2) to describe program content, dose and structure, informing better design and dissemination. METHODS Using PRISMA guidelines, a database search up to January 2018, yielded 1889 unique articles, screened by 2 authors; 9 studies met inclusion criteria and were retained for analysis. RESULTS 100% of studies reported significant improvements in BCC skills among physicians, most programs targeting provider-patient collaboration, supporting patient autonomy, and use of open questions to elicit "change-talk". Limitation included: poor reporting quality, high program heterogeneity, small sample sizes, 78% of studies having no comparison group, and less than 30% of skills taught being formally assessed. CONCLUSION Training programs were efficacious, but methodological weaknesses limit the ability to determine content and delivery. Caution is necessary when interpreting the results. PRACTICE IMPLICATIONS Further research emphasizing rigorous training program development and testing is warranted.

['Dragomir|Anda I|AI|', 'Julien|Cassandre A|CA|', 'Bacon|Simon L|SL|', 'Boucher|Vincent Gosselin|VG|', 'Lavoie|Kim L|KL|', '|||']

2019

33752654

Exploring the pedagogical design features of the flipped classroom in undergraduate nursing education: a systematic review.

BACKGROUND In recent years, technological advancement has enabled the use of blended learning approaches, including flipped classrooms. Flipped classrooms promote higher-order knowledge application - a key component of nursing education. This systematic review aims to evaluate the empirical evidence and refereed literature pertaining to the development, application and effectiveness of flipped classrooms in reference to undergraduate nursing education. METHODS A PRISMA systematic review protocol was implemented to investigate the literature pertaining to the development, implementation and effectiveness of flipped classroom pedagogy in undergraduate nursing education. Seven databases (Scopus, PsycINFO, CINAHL, ERIC, MEDLINE, Cochrane, Web of Science) were utilised to survey the salient literature. Articles were appraised with respect to their level of evidence, the origin of study, study design, the aims/s of the study, and the key outcomes of the study. A qualitative synthesis was then conducted to summarise the study findings. RESULTS The initial search identified 1263 potentially relevant articles. After comprehensively reviewing the initial catchment using several analytical phases, 27 articles were considered for the final review, most of which were conducted in the USA and South Korea. A range of research designs were applied to measure or discuss the outcomes and design features of the flipped classroom pedagogy when applied to undergraduate nursing education. The review indicated that a common operational flipped classroom model involves three key components, namely pre-classroom activities, in-classroom activities and post-classroom activities, guided by two instructional system design principles. The review predominantly identified positive learning outcomes among undergraduate nursing students, after experiencing the flipped classroom, in terms of skills, knowledge and attitudes. However, a few studies reported contrasting findings, possibly due to the incompatibility of the flipped classroom pedagogy with the traditional learning culture. CONCLUSIONS Current evidence in this systematic review suggests that incorporating the flipped classroom pedagogy could yield positive educational outcomes in undergraduate nursing education. There are promising pedagogical models available for adapting or developing the flipped classroom pedagogy in undergraduate nursing education.

['Youhasan|Punithalingam|P|http://orcid.org/0000-0002-3435-7839', 'Chen|Yan|Y|', 'Lyndon|Mataroria|M|', 'Henning|Marcus A|MA|']

2021

26908827

TLR4, TLR7/8 agonist-induced miR-146a promotes macrophage tolerance to MyD88-dependent TLR agonists.

TLRs facilitate the recognition of pathogens by immune cells and the initiation of the immune response, leading to the production of proinflammatory cytokines and chemokines. Production of proinflammatory mediators by innate immune cells, such as macrophages, is tightly regulated to facilitate pathogen clearance while limiting an adverse impact on host tissue. Exposure of innate immune cells to TLR ligands induces a state of temporary refractoriness to a subsequent exposure of a TLR ligand, a phenomenon referred to as "tolerance." This study sought to evaluate the mechanistic regulation of TLR4 and TLR7/8 ligand-induced tolerance to other TLRs by microRNA-146a. With the use of THP-1 macrophages, as well as human classic and alternative macrophages, we demonstrate that priming with a TLR4 agonist (LPS) or a TLR7/8 agonist (R848) induces homologous and heterologous tolerance to various TLR ligands in macrophages, leading to the impaired production of cytokines and chemokines. We also demonstrate that overexpression of microRNA-146a is sufficient to mimic LPS or R848-induced hyporesponsiveness. Conversely, inhibition of microRNA-146a activity leads to LPS- or R848-induced TLR hyper-responsiveness in TLR signaling tolerance. Furthermore, we demonstrate that microRNA-146a dampens cytokine production following a primary stimulus with MyD88-dependent but not MyD88-independent TLR pathways. Collectively, these data provide comprehensive evidence of the central role of microRNA-146a in TLR signaling tolerance to plasma membrane, as well as endosomal TLR ligands in human macrophages.

['Nahid|M Abu|MA|', 'Benso|Lia M|LM|', 'Shin|John D|JD|', 'Mehmet|Huseyin|H|', 'Hicks|Alexandra|A|', 'Ramadas|Ravisankar A|RA|']

2016

22950037

Clinical features of allergic bronchopulmonary aspergillosis in Korea.

Allergic bronchopulmonary aspergillosis (ABPA) is a complex disease, triggered by a hypersensitivity reaction to the allergen Aspergillus fumigatus. This disease occurs frequently in patients with cystic fibrosis and severe asthma in Western countries, with a prevalence of 2%-15%. However, there have been only a few case reports in Korea. We investigated the clinical and immunological features of patients with ABPA. Ten adult patients diagnosed with ABPA, according to Greenberger's criteria, were analyzed during the period January 2001 to December 2010 in a tertiary hospital. Skin-prick tests, pulmonary function tests, and high-resolution computed tomography (HRCT) were performed, and total serum IgE and A. fumigatus-specific IgE were measured. The patient cohort consisted of men who were middle-aged (median, 62.5; range, 19.0-79.0 years) at the diagnosis of ABPA with a long duration of asthma (median, 15.0; range, 1-48 years). Approximately 40% of the patients had a history of pulmonary tuberculosis more than 10 years prior to the study (median 23.5; range, 10.0-31.0 years) accompanied by severe obstructive lung function and radiological post-tuberculous destructive lung lesions. These patients also tended to have increased levels of immunologic parameters, such as total eosinophil count, total IgE, and A. fumigates-specific IgE, compared to those without tuberculosis sequels. Two patients with steroid-dependent asthma were treated with anti-IgE therapy and showed good responses. We report the clinical features of 10 ABPA patients, including 4 with histories of post-tuberculosis destructive lesions. Furthermore, anti-IgE antibody therapy may be an alternative strategy in cases of steroid-dependent ABPA.

['Kim|Joo-Hee|JH|', 'Jin|Hyun Jung|HJ|', 'Nam|Young-Hee|YH|', 'Hwang|Eui-Kyung|EK|', 'Ye|Young-Min|YM|', 'Park|Hae-Sim|HS|']

2012

25724302

Roles of nonclinical and clinical data in prediction of 30-day rehospitalization or death among heart failure patients.

BACKGROUND Selecting heart failure (HF) patients for intensive management to reduce readmissions requires effective targeting. However, available prediction scores are only modestly effective. We sought to develop a prediction score for 30-day all-cause rehospitalization or death in HF with the use of nonclinical and clinical data. METHODS AND RESULTS This statewide data linkage included all patients who survived their 1st HF admission (with either reduced or preserved ejection fraction) to a Tasmanian public hospital during 2009-2012. Nonclinical data (n = 1,537; 49.5% men, median age 80 y) included administrative, socioeconomic, and geomapping data. Clinical data before discharge were available from 977 patients. Prediction models were developed and internally and externally validated. Within 30 days of discharge, 390 patients (25.4%) died or were rehospitalized. The nonclinical model (length of hospital stay, age, living alone, discharge during winter, remoteness index, comorbidities, and sex) had fair discrimination (C-statistic 0.66 [95% confidence interval (CI) 0.63-0.69]). Clinical data (blood urea nitrogen, New York Heart Association functional class, albumin, heart rate, respiratory rate, diuretic use, angiotensin-converting enzyme inhibitor use, arrhythmia, and troponin) provided better discrimination (C-statistic 0.72 [95% CI 0.68-0.76]). Combining both data sources best predicted 30-day rehospitalization or death (C-statistic 0.76 [95% CI 0.72-0.80]). CONCLUSIONS Clinical data are stronger predictors than nonclinical data, but combining both best predicts 30-day rehospitalization or death among HF patients.

['Huynh|Quan L|QL|', 'Saito|Makoto|M|', 'Blizzard|Christopher L|CL|', 'Eskandari|Mehdi|M|', 'Johnson|Ben|B|', 'Adabi|Golsa|G|', 'Hawson|Joshua|J|', 'Negishi|Kazuaki|K|', 'Marwick|Thomas H|TH|', '|||']

2015

30536699

Characterization of a novel method for the production of single-span membrane proteins in Escherichia coli.

The large-scale production and isolation of recombinant protein is a central element of the biotechnology industry and many of the products have proved extremely beneficial for therapeutic medicine. Escherichia coli is the microorganism of choice for the expression of heterologous proteins for therapeutic application, and a range of high-value proteins have been targeted to the periplasm using the well characterized Sec protein export pathway. More recently, the ability of the second mainstream protein export system, the twin-arginine translocase, to transport fully-folded proteins into the periplasm of not only E. coli, but also other Gram-negative bacteria, has captured the interest of the biotechnology industry. In this study, we have used a novel approach to block the export of a heterologous Tat substrate in the later stages of the export process, and thereby generate a single-span membrane protein with the soluble domain positioned on the periplasmic side of the inner membrane. Biochemical and immuno-electron microscopy approaches were used to investigate the export of human growth hormone by the twin-arginine translocase, and the generation of a single-span membrane-embedded variant. This is the first time that a bonafide biotechnologically relevant protein has been exported by this machinery and visualized directly in this manner. The data presented here demonstrate a novel method for the production of single-span membrane proteins in E. coli.

['Smith|Sarah M|SM|0000-0002-5349-1902', 'Walker|Kelly L|KL|', 'Jones|Alexander S|AS|', 'Smith|Corinne J|CJ|', 'Robinson|Colin|C|0000-0002-1739-032X']

2019

30955661

Comparison of biochemical properties of membrane-bound and soluble polyphenol oxidase from Granny Smith apple (Malus × domestica Borkh.).

Polyphenol oxidase from Granny Smith apples was purified and characterized in both its soluble form (sPPO) and its membrane-bound form (mPPO). Both forms were purified by temperature-induced phase partitioning, precipitation with ammonium sulfate, and ion exchange chromatography. The specific activity of mPPO was 19.17 times that of sPPO. The optimum pH and temperature for both forms were 7.0 and 35 °C when catechol was the substrate. The Michaelis constant and maximum reaction rate for sPPO were 34.1 mM and 500 U/mL/min, whereas those for mPPO were 53 mM and 10,000 U/mL/min, respectively. The enzymes exhibited diphenolase activity, and their affinity was highest for catechol (sPPO) and 4-methylcatechol (mPPO). Inhibitors of sPPO and mPPO included ascorbic acid, glutathione, and l-cysteine. However, ethylenediaminetetraacetic acid increased the activity of mPPO. Purified sPPO was dimeric with a molecular weight of 31 kDa, whereas mPPO was monomeric with an estimated molecular weight of 65 kDa.

['Han|Qian-Yun|QY|', 'Liu|Fang|F|', 'Li|Mo|M|', 'Wang|Kun-Li|KL|', 'Ni|Yuan-Ying|YY|']

2019

31161941

Nebulized Tranexamic Acid Therapy for Hemoptysis Associated with Submassive Pulmonary Embolism.

Hemoptysis and pulmonary embolism (PE) are life-threatening pulmonary emergencies that, when present together, create a therapeutic conundrum. We present an illustrative case of a 65-year-old man with unprovoked submassive PE and moderate hemoptysis due to pulmonary infarction. Hemoptysis precluded systemic anticoagulation. Failing a conservative management strategy, we administered nebulized tranexamic acid. After four doses of nebulized tranexamic acid 500 mg, 6 hours apart, hemoptysis had ceased. Systemic anticoagulation with intravenous heparin was then successfully commenced 12 hours after the last episode of hemoptysis. The patient was weaned off high-flow nasal oxygen therapy over the course of the next 5 days with no hemoptysis recurrence. Noting the absence of trial evidence, but good pharmacological rationale and our positive experience, we suggest tranexamic acid is a useful noninvasive treatment option for the management of such conditions. Consent for this publication was obtained from the patient.

['Dhanani|Jayesh A|JA|', 'Roberts|Jason|J|', 'Reade|Michael C|MC|']

2020

26451503

Hippocampal volume in vulnerability and resilience to depression.

BACKGROUND Reduced hippocampal volume has been associated with clinical depression. However, it remains unclear whether these changes are a biological vulnerability marker or a consequence of this disorder. METHODS AND RESULTS (STUDY 1): We first compared hippocampal volumes between (i) never-depressed individuals with elevated risk for depression by virtue of high neuroticism (ii) recovered depressed individuals with matched levels of neuroticism; and (iii) individuals with low neuroticism and no history of depression. We replicated the finding of reduced hippocampal volume in the recovered group; unexpectedly however, the never-depressed high-risk group showed an increase in volume. One hypothesis is that this group had a mean age above the typical onset age for depression; hence, these participants who have remained euthymic despite their personality risk might in fact possess some resilience. METHODS AND RESULTS (STUDY 2): A subsequent study was therefore carried out to compare hippocampal volume between high-neurotic vs. low-neurotic volunteers in a younger sample. No group difference was found. LIMITATIONS The present findings are limited by a small sample size; the cross-sectional design precluded us from makineg definitive conclusions about causal effect. CONCLUSION Our overall results suggest that reduced hippocampal volumes is a neural marker for the scar effect of depression, although this structural impairment could also be seen as a vulnerability marker for the development of future recurrent episodes. By contrast, larger hippocampal volumes could be a biological marker of resilience. These findings have clinical implications regarding treatment development for the prevention of illness onset and recurrent depressive episodes.

['Chan|Stella W Y|SW|', 'Harmer|Catherine J|CJ|', 'Norbury|Ray|R|', "O'Sullivan|Ursula|U|", 'Goodwin|Guy M|GM|', 'Portella|Maria J|MJ|']

2016

30555929

Evaluation of the McMaster Family Health Team: results and practical implications for quality improvement.

Purpose To evaluate the McMaster Family Health Team (MFHT) as part of a Continuous Quality Improvement initiative using a set of provincial performance metrics to demonstrate which measures of assessment are actually clinically meaningful in context and where system-level changes might be implemented to improve operational practice. Methods Measures were selected from the Primary Care Performance Measurement Framework based on data availability for the MFHT and provincial comparators. The measures explored in this paper are those that were deemed to have actionable properties. Data were extracted from billing reports, electronic medical records and information collated for the Association of Family Health Teams of Ontario Data to Decisions database. Metrics were then examined to demonstrate the importance of interpretation in clinical context. Conclusions Quantitative assessment of performance based on standardised measures is a suitable starting point when evaluating a practice, however it is not appropriate as a stand-alone report card of practice performance. Rather, quantitative measures must be of clinical relevance and applicable to the patient populations of interest in order to create conversation and impact change. Thus, the focus of quality improvement should not be to improve numbers relating to efficiency, patient satisfaction and continuity of care, but rather to determine what drives those numbers and how changes might be made at a system or practice level that will optimise clinician buy-in.

['Nasser|Laila|L|', 'Stosic|Alix|A|', 'Price|David|D|']

2018

33799088

Surgical treatment and clinical outcome of large pediatric interhemispheric cysts with callosal agenesis: A systematic literature review with four additional patients.

OBJECTIVE Large interhemispheric cysts (IHC) with partial or complete agenesis of corpus callosum (ACC) constitute a heterogeneous group of rare disorders. Neurosurgical treatment, in the terms of if, when and how to operate, remains unclear METHODS: We performed a surgical literature review of series or reports of IHCs with callosal anomalies; we evaluated whether revision surgeries were necessary and considered the dimensional change in the cyst postoperatively and the developmental outcome. We also reported our experience with sfour patients treated by programmable cysto-peritoneal (CP) shunting. Patients' clinical history, neuroradiological and neuropsychological performances were evaluated pre and post operatively. RESULTS The review included 133 patients with surgically-treated IHCs. Although most authors are in agreement to perform surgery if the patients present signs of raised ICP and to not intervene in those completely asymptomatic, for other signs and symptoms the debate is still open; only few authors performed cognitive tests pre and post-operatively. Shunting procedures were successful in 60% of our reviewed cases and often lead to a major cyst collapse. Craniotomy achieves good results but is extremely invasive. Endoscopy is minimally invasive and our review demonstrated a success rate of 66%. However, endoscopy does not ensue a complete cyst collapse. Our series and review seem to suggest a possible link between parenchymal re-expansion and cognitive outcome. CONCLUSIONS Early and effective surgery seems to obtain a greater cerebral parenchyma re-expansion and long-term cognitive evolution. Endoscopy is safe and reliable, but more data is needed on the impact of uncomplete cyst collapse on neurocognitive outcome.

['Ferrera|Giulia|G|', 'Vetrano|Ignazio G|IG|', 'Chiapparini|Luisa|L|', 'Moscatelli|Marco|M|', 'Taddei|Matilde|M|', 'Erbetta|Alessandra|A|', 'Valentini|Laura G|LG|', 'Saletti|Veronica|V|']

2021

30933795

Reduction of nitrate by zero valent iron (ZVI)-based materials: A review.

Zero valent iron (ZVI) and ZVI-based materials have been widely used for the reduction of nitrate, a major contaminant commonly detected in groundwater and surface water. The reduction of nitrate by ZVI is influenced by various factors, such as the physical and chemical characteristics of ZVI and the operational parameters. There are some problems for the nitrate reduction by ZVI alone, for example, the formation of iron oxides on the surface of ZVI at high pH condition, which will inhibit the further reduction of nitrate; in addition, the end reduction product is mainly ammonium, which itself needs to be concerned. Several strategies, such as the optimization of the structure of ZVI composites and the addition of reducing assistants, have been proposed to increase the reduction efficiency and the selectivity of end product of nitrate reduction in a wide range of pH, especially under neutral pH condition. This review will mainly focus on the high efficient reduction of nitrate by ZVI-based materials. Firstly, the reduction of nitrate by ZVI alone was briefly introduced and discussed, including the influence of physical and chemical characteristics of ZVI and some operational parameters on the reduction efficiency of nitrate. Then, the strategies for enhancing the reduction efficiency and the N2 selectivity of the reductive products of nitrate were systematically analyzed and evaluated, especially the optimization of the structure of ZVI composites (e.g., doped ZVI composite, supported ZVI composite and premagnetized ZVI), and the addition of reducing assistants (e.g., metal cations, ligand, hydrogen gas and light) were highlighted. Thirdly, the mechanisms and pathways of nitrate reduction were discussed. Finally, concluding remarks and some suggestions for the future research were proposed.

['Liu|Yong|Y|', 'Wang|Jianlong|J|']

2019

30852220

Investigation on the near-field evolution of industrial plumes from metalworking activities.

In a context where a significant fraction of the population lives near industrial areas, the main objectives of this study are to provide (a) new data on PM2.5 chemical compositions, heavy-metal concentrations and trace gases released by metalworking activities and (b) new information on the near-field evolution (up to about a thousand meters) of such industrial plumes in terms of particle chemical composition and size distribution. For that purpose, a one-month field campaign was performed in an industrial area near the city of Dunkirk (Northern France), combining measurements of atmospheric dynamics and physico-chemical characterization of air masses. Comparisons between several elemental ratios (mainly Mn/Fe), particle size distributions and volatile organic compound (VOC) concentrations at the stacks and at a near-field site suggest that plumes of a ferromanganese alloy plant were quickly mixed with pollutants emitted by other sources (mainly other industries, possibly traffic and sea spray), in particular a neighboring steelworks, before reaching the sampling site. This led to the emergence of secondary particles related to condensation and/or aggregation phenomena inside the plumes. Metalworking emissions were also identified as a source of new particle formation, formed through the emission of gaseous precursors and their fast transformation and condensation, over a timescale of minutes before reaching the near-field site 800 m downwind. Ultrafine particles emitted at the stacks also quickly agglomerated to form larger particles before reaching the near-field site. These results show that, even over short distances, the chemical composition and size distribution of metalworking plumes may evolve rapidly and the characteristics of particles at the boundary of an industrial area (especially in contiguous urban areas) may differ from those emitted directly at the stacks.

['Setyan|Ari|A|', 'Flament|Pascal|P|', 'Locoge|Nadine|N|', 'Deboudt|Karine|K|', 'Riffault|Véronique|V|', 'Alleman|Laurent Y|LY|', 'Schoemaecker|Coralie|C|', 'Arndt|Jovanna|J|', 'Augustin|Patrick|P|', 'Healy|Robert M|RM|', 'Wenger|John C|JC|', 'Cazier|Fabrice|F|', 'Delbarre|Hervé|H|', 'Dewaele|Dorothée|D|', 'Dewalle|Pascale|P|', 'Fourmentin|Marc|M|', 'Genevray|Paul|P|', 'Gengembre|Cyril|C|', 'Leonardis|Thierry|T|', 'Marris|Hélène|H|', 'Mbengue|Saliou|S|']

2019

27274504

An online questionnaire survey on preferred timing for the diagnosis and management of thyroid carcinoma in general population in Korea.

PURPOSE An optimal timing for diagnosis and management of papillary thyroid microcarcinoma (PTMC) has become the subject for much controversy. The aim of the present study is to analyze people's preference in Korea for timing of diagnosis and management of PTMC using an online questionnaire. METHODS The questionnaire consists of 3 questions about preference for the diagnosis and management of PTMC and 3 additional questions about respondents' personal information. An online survey was conducted from March 3 to June 3 in 2015 using Google Survey (http://goo.gl/forms/b81yEjqNUA). RESULTS A total 2,308 persons (1,246 males, 1,053 females) answered the questionnaire. Respondents' ages varied widely from teenagers to 70-year-olds. If there was a suspicious thyroid nodule from PTMC measuring less than 1 cm in diameter, 95.7% of respondents want to know a cytological diagnosis for it. If a thyroid nodule turned out to be a PTMC, 59.5% of respondents wanted it removed immediately. For surgical management of PTMC, 53.0% of respondents were worried more about recurrences than complications. In subgroup analyses, respondents younger than 40 years old more often want immediate surgery than others: 66.7% vs. 32.7% (P < 0.05). Respondents who underwent thyroid cancer surgery (n = 91) were worried more about recurrences than others: 69.2% vs. 52.4% (P < 0.05). CONCLUSION Almost all respondents in the present study wanted diagnosis of suspicious thyroid nodules immediately. However, there were opposing opinions about the preferred timing for surgical treatment and surgical extents. A patient's right to know their disease status and decision on treatments should be emphasized all the more.

['Park|Jin-Woo|JW|', 'Yoo|Jae-Soo|JS|', 'Yun|Jin-Kyung|JK|', 'Kim|Byoung-Hoon|BH|', 'Noh|Yeon-Woo|YW|', 'Kim|Dong-Ju|DJ|']

2016

23772874

CYP3A activity in severe liver cirrhosis correlates with Child-Pugh and model for end-stage liver disease (MELD) scores.

AIMS Impaired liver function often necessitates drug dose adjustment to avoid excessive drug accumulation and adverse events, but a marker for the extent of the required adjustment is lacking. The aim of this study was to investigate whether Child-Pugh (CP) and model for end-stage liver disease (MELD) scores correlate with drug clearance. METHODS Midazolam was used as a CYP3A probe and its pharmacokinetics were analyzed in 24 patients with mild to severe liver cirrhosis (n = 4, 10 and 10 with CP class A, B and C, respectively) and six patients without liver disease. RESULTS Both scores correlated well with unbound midazolam clearance (CLu ), unbound midazolam fraction and half-life (all P < 0.01), whereas the unbound steady-state volume of distribution was not significantly changed. In patients with severe liver cirrhosis unbound midazolam clearance was only 14% of controls (CP C: CLu = 843 ± 346 l  h(-1), MELD ≥ 15: CLu = 805 ± 474 l  h(-1), controls: CLu = 5815 ± 2649 l  h(-1), P < 0.01). CONCLUSION The correlation with unbound midazolam clearance suggests that either score predicts the metabolic capacity of CYP3A, the most relevant drug metabolizing enzyme subfamily in humans.

['Albarmawi|Albader|A|', 'Czock|David|D|', 'Gauss|Annika|A|', 'Ehehalt|Robert|R|', 'Lorenzo Bermejo|Justo|J|', 'Burhenne|Jürgen|J|', 'Ganten|Tom M|TM|', 'Sauer|Peter|P|', 'Haefeli|Walter E|WE|']

2014

28039035

Inhibitory effects of sodium azide on microbial growth in experimental resuspension of marine sediment.

Sodium azide (NaN3) was evaluated as inhibitor of microbial growth and activity in marine sediment resuspensions by monitoring the abundance of free-living and sessile bacteria using both flow cytometry and qPCR methods. Results show that 50mM of NaN3 strongly inhibits bacterial growth under natural and enriched resource conditions.

['Cabrol|Léa|L|', 'Quéméneur|Marianne|M|', 'Misson|Benjamin|B|']

2017

28843345

Cyclosporine-immunosuppression does not affect survival of transplanted skin-derived precursor Schwann cells in the injured rat spinal cord.

A major goal of Schwann cell (SC) transplantation for spinal cord injury (SCI) is to fill the injury site to create a bridge for regenerating axons. However, transplantation of peripheral nerve SCs requires an invasive biopsy, which may result in nerve damage and donor site morbidity. SCs derived from multipotent stem cells found in skin dermis (SKP-SCs) are a promising alternative. Regardless of source, loss of grafted SCs post-grafting is an issue in studies of regeneration, with survival rates ranging from ∼1 to 20% after ≥6 weeks in rodent models of SCI. Immune rejection has been implicated in these low survival rates. Therefore, our aim was to explore the role of the immune response on grafted SKP-SC survival in Fischer rats with a spinal hemisection injury. We compared SKP-SC survival 6 weeks post-transplantation in: (I) cyclosporine-immunosuppressed rats (n=8), (II) immunocompetent rats (n=9), and (III) rats of a different sub-strain than the SKP-SC donor rats (n=7). SKP-SC survival was similar in all groups, suggesting immune rejection was not a main factor in SKP-SC loss observed in this study. SKP-SCs were consistently found on laminin expressed at the injury site, indicating detachment-mediated apoptosis (i.e., anoikis) might play a major role in grafted cell loss.

['May|Zacnicte|Z|', 'Torres-Espín|Abel|A|', 'Lucas-Osma|Ana M|AM|', 'Batty|Nicholas J|NJ|', 'Raposo|Pamela|P|', 'Fenrich|Keith K|KK|', 'Stykel|Morgan G|MG|', 'Führmann|Tobias|T|', 'Shoichet|Molly|M|', 'Biernaskie|Jeff|J|', 'Fouad|Karim|K|']

2017

23112503

Occupational stress, mental health and coping among information technology professionals.

BACKGROUND Experience of occupational stress is inevitably involved in the execution of any type of work. Stress has an adaptive value. It motivates the individual to attend to the task and get rid of the tension or demand the unattended task produced. MATERIALS AND METHODS The study was planned to investigate the differences between executives and shop floor workers on occupational stress, mental health, job satisfaction and coping. A random sample of 200 executives and shop floor employees collected from Nuclear Fuel Complex of Hyderabad City. A well developed sub-scales of Occupational Stress indicator like Mental Health, and Coping behavior were used in the present study. RESULTS AND CONCLUSION The shop floor workers experiencing more job stress and lower mental health. But these two groups did not differ in their coping behaviour. The executives are better with work home balance.

['Rao|Jakkula V|JV|', 'Chandraiah|K|K|']

2012

31623999

Large cemented gibbsite agglomerates in alkaline nuclear waste at the Hanford site and the impacts to remediation.

Recent work with the remediation of legacy alkaline nuclear waste has focused on nanometer and micrometer particle sizes, emphasizing how these small particles can impact efforts to treat the waste. Building upon this work, we present here findings that show very large particles (several centimeters in size) also exist in these waste which likewise play an important role in the remediation process. While large cemented gibbsite nodules have been periodically reported in acid soils in the literature, this study found similar large gibbsite agglomerates (7 cm in diameter) in alkaline nuclear waste, the first time that such large agglomerates have been identified in an alkaline environment. The morphology of the gibbsite in the agglomerates that were grown over more than 40 years of storage in the waste tank were similar to the much smaller agglomerates that have been reported in previous shorter term studies. Fluid dynamics calculations indicate that these cemented particles would be difficult to mobilize with standard jet slurry technologies, which is consistent with their persistence in the waste heel after jet sluicing of the tank.

['Page|Jason S|JS|', 'Reynolds|Jacob G|JG|', 'Cooke|Gary A|GA|', 'Wells|Beric E|BE|']

2020

34845729

Information flow in the spatiotemporal organization of immune responses.

Immune responses must be rapid, tightly orchestrated, and tailored to the encountered stimulus. Lymphatic vessels facilitate this process by continuously collecting immunological information (ie, antigens, immune cells, and soluble mediators) about the current state of peripheral tissues, and transporting these via the lymph across the lymphatic system. Lymph nodes (LNs), which are critical meeting points for innate and adaptive immune cells, are strategically located along the lymphatic network to intercept this information. Within LNs, immune cells are spatially organized, allowing them to efficiently respond to information delivered by the lymph, and to either promote immune homeostasis or mount protective immune responses. These responses involve the activation and functional cooperation of multiple distinct cell types and are tailored to the specific inflammatory conditions. The natural patterns of lymph flow can also generate spatial gradients of antigens and agonists within draining LNs, which can in turn further regulate innate cell function and localization, as well as the downstream generation of adaptive immunity. In this review, we explore how information transmitted by the lymph shapes the spatiotemporal organization of innate and adaptive immune responses in LNs, with particular focus on steady state and Type-I vs. Type-II inflammation.

['Huang|Jessica Y|JY|', 'Lyons-Cohen|Miranda R|MR|', 'Gerner|Michael Y|MY|https://orcid.org/0000-0001-5406-8308']

2021

30936746

Oncogenic role of ABHD5 in endometrial cancer.

Background Abhydrolase domain containing 5 (ABHD5) functions as a tumor suppressor in colorectal and prostate cancers. The aim of this study was to investigate the roles of ABHD5 in endometrial cancer. Materials and methods ABHD5 expression was detected in clinical samples by immunohistochemical staining. Cell proliferation and invasion were evaluated with the Cell Counting Kit-8 and Transwell assay, respectively. Western blotting was performed to analyze protein expression. Glucose uptake was assessed by 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose. Lactate production was detected by a lactate assay kit. Results In the present study, ABHD5 was overexpressed in endometrial cancer tissues, and its expression was closely correlated with the International Federation of Gynecology and Obstetrics (FIGO) stage and lymph node metastasis. In addition, we observed that the knockdown of ABHD5 inhibited cell proliferation, invasion, glucose uptake and lactate production in HEC-1A cells, which expressed high levels of ABHD5. Conversely, the opposite effects were observed when ABHD5 was ectopically expressed in Ishikawa cells, which had low levels of ABHD5. Furthermore, the changes in glycolysis regulators (enolase 1 [ENO1], glucose transporter 1 [GLUT1] and lactate dehydrogenase A [LDHA]) and epithelial-to-mesenchymal transition-related proteins (E-cadherin and Snail) in HEC-1A cells with ABHD5 knockdown were consistent with the effects of ABHD5 on glycolysis and cell invasion. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was increased, while the phosphorylated AKT (p-AKT) was decreased when ABHD5 was downregulated. Notably, treatment with the allosteric AKT inhibitor MK-2206 completely abolished the effects caused by ABHD5 overexpression in Ishikawa cells. Finally, ABHD5 knockdown potently suppressed tumor growth in vivo. Conclusion Overall, these results suggest that ABHD5 may play an oncogenic role in endometrial cancer via the AKT pathway.

['Zhou|Qing|Q|', 'Wang|Fang|F|', 'Zhou|Kai|K|', 'Huang|Kate|K|', 'Zhu|Qiuyuan|Q|', 'Luo|Xishao|X|', 'Yu|Jiangtao|J|', 'Shi|Zhengzheng|Z|']

2019

31865444

Drug reactions in children with rheumatic diseases receiving parenteral therapies: 9 years' experience of a tertiary pediatric rheumatology center.

Parenteral treatments (either subcutaneous or intravenous) are frequently used in rheumatology practice. In this study, drug side effects in patients who were followed up with a rheumatic disease and treated with parenteral administration methods were evaluated. The drug side effects in children who were followed up with a rheumatic disease and treated with parenteral treatments between 2010 and 2019 were recorded, retrospectively. All parenteral treatments are applied by a clinical nurse specialist (CNS) who is experienced in pediatric rheumatology for 10 years. Four hundred and thirteen patients were evaluated in this study. The mean age was 12.09 ± 5.05 years. Most of them were diagnosed with juvenile idiopathic arthritis (n = 317) and colchicine-resistant familial Mediterranean fever (n = 57). Among the patients, 287 was treated with methotrexate, 130 with etanercept, 90 with adalimumab, 71 with anakinra, 64 with canakinumab, 55 with tocilizumab, seven with rituximab, six with infliximab, and four with abatacept. Two of the patients had a history of drug allergy (ceftriaxone = 1, ranitidine = 1). The most common adverse reactions were as follows: nausea-vomiting in 52, rash in 11, itching in three, chest tightening in two, bruising in two, headache in two, and abdominal pain in one of the patients. Drug side effects were observed after an average of three (1-4) administrations. Antihistaminic and steroids (tocilizumab = 3, infliximab = 1, methotrexate = 1) were administered to five patients due to hypersensitivity reactions. Considering the possible side effects and preparation protocols of parenteral treatments, experienced physicians and nurses are required in the field.

['Koç|Rahime|R|http://orcid.org/0000-0002-9927-1526', 'Sönmez|Hafize Emine|HE|http://orcid.org/0000-0002-9186-3068', 'Çakan|Mustafa|M|', 'Karadağ|Şerife Gül|ŞG|http://orcid.org/0000-0002-3232-0055', 'Tanatar|Ayşe|A|', 'Çakmak|Figen|F|http://orcid.org/0000-0002-1667-2480', 'Aktay Ayaz|Nuray|N|http://orcid.org/0000-0003-3594-7387']

2020

33362923

The role of red blood cell exchange in sickle cell disease in patient with COVID-19 infection and pulmonary infiltrates.

Due to the overlap between ACS and COVID-19 pneumonia, we recommend close monitoring for those patients and offering them RBC exchange early in the course of the disease to avoid clinical deterioration.

['Okar|Lina|L|https://orcid.org/0000-0001-7184-1235', 'Aldeeb|Maya|M|', 'Yassin|Mohamed A|MA|https://orcid.org/0000-0002-1144-8076']

2020

25565754

Perceptions of Indian dental hygiene students toward their profession and its relationship with their explicit self-esteem scores.

BACKGROUND The purpose of this study was to investigate the perceptions toward the profession, the level of explicit self-esteem (ESE) of Indian students pursuing the course of dental hygienists, to evaluate the relationship between the two and to develop educational strategies to positively influence students' perceptions. We also wished to evaluate the level of satisfaction of the students to the current status of professional employment in the country. MATERIALS AND METHODS Students in the second year of the dental hygienist 2-year course were asked to participate in a cross-sectional survey study. An instrument was used to obtain students' perceptions about the profession by estimating the dimensions of "Motivation," "Expectation" and "Environment". Their self-esteem was evaluated using the Rosenberg self-esteem scale. Relationship of self esteem scores with perceptions towards profession was then evaluated. RESULTS Scores for dimensions including "Motivation," "Expectation" and "Environment" were significantly high, as were the self-esteem scores. The level of ESE was positively correlated with their perceptions of the profession. CONCLUSIONS The perception of the Indian dental hygienist students was significantly high and positively correlated to the ESE scores. We also conclude that environmental factors may be more influential than innate cultural factors for the development of self-esteem.

['Gupta|Shipra|S|', 'Jain|Ashish|A|', 'Garg|Sakshi|S|', 'Sood|Shaveta|S|', 'Kumari|Bindiya|B|']

2014

32784266

Venous thromboembolism in patients with adrenocortical carcinoma after surgery.

Background Adrenocortical carcinoma is a rare malignancy with a poor prognosis. We hypothesized that patients with adrenocortical carcinoma are at high risk for venous thromboembolism, given the numerous risk factors such as malignancy, abdominal surgery, immobility and hormonal excess. The aim of this study was to determine retrospectively the incidence of venous thromboembolisms after surgical treatment in patients with adrenocortical carcinoma. Materials and methods A retrospective study was performed, collecting data from all patients diagnosed with adrenocortical carcinoma from 2003 to 2018 at the Radboud University Medical Centre, The Netherlands. Results In 34 patients, eight postoperative venous thromboembolisms, all pulmonary embolisms, were diagnosed in the first 6 months after adrenalectomy (23.5%). In addition, one patient developed pulmonary embolism just prior to surgery and one patient 7 years after surgery. Five of the eight patients with postoperative venous thromboembolisms presented with symptomatic pulmonary embolism whereas the other three pulmonary embolisms were incidentally found on regular follow up CT scans. Seven of the eight venous thromboembolisms occurred within 10 weeks after surgery. Seven of the eight patients had advanced stage adrenocortical carcinoma and four patients already received low-molecular weight heparin during the development of the venous thromboembolism. There was one case of fatal pulmonary embolism in a patient with a cortisol producing tumor with pulmonary metastases, despite the use of a therapeutic dose thromboprophylaxis. Conclusion Patients with adrenocortical carcinoma are at high risk of developing postoperative venous thromboembolisms. Prolonged postoperative thromboprophylaxis could be considered in these patients.

['Durmuşoğlu|Jülide|J|', 'Timmers|Henri J L M|HJLM|', 'van Houten|Pepijn|P|0000-0002-8503-4140', 'Langenhuijsen|Johan F|JF|', 'Hermus|Ad R M M|ARMM|', 'van de Ven|Annenienke C|AC|']

2020

33527828

Solution-Processed Donor-Acceptor Poly(3-hexylthiophene):Phenyl-C61-butyric Acid Methyl Ester Diodes for Low-Voltage α Particle Detection.

Diodes fabricated using a blend of poly(3-hexylthiophene) and phenyl-C61-butyric acid methyl ester (6-80 μm thick) as an organic semiconductor component achieved consistent 4 MeV α particle detection. Current-voltage characteristics and current-time measurements were obtained under α irradiation and in its absence. Steady-state and transient (time-of-flight) photoconduction measurements were additionally performed. Low-bias (<20 V) α particle detection gain-efficiency products of order 10-2 were measured. The α particle detection was achieved reproducibly, reversibly, and repeatably in different devices of varying organic semiconductor layer thicknesses using both the steady-state and time-dependent (dynamic) diode responses. Conductive gain, due to trapped electrons, increased the α particle gain-efficiency product in both forward and reverse bias conditions as well as increasing steady-state photoconduction. The device thickness was optimized to maximize the gain-efficiency product by matching the penetration depth of the α particle, obtained by modeling, to the organic semiconductor layer thickness. Very high confidence α particle detection was achieved (with signal-to-noise ratios exceeding 20) under optimized device dimensions and drive conditions. Hecht function fitting of the gain-efficiency product versus electric field data returns mobility-lifetime products of order 10-6-10-7 cm2 V-1. This work demonstrates that solution-processed organic semiconductor diodes are viable for low-voltage α particle detection.

['Taifakou|Fani Eirini|FE|', 'Ali|Muhammad|M|', 'Borowiec|Joanna|J|', 'Liu|Xiaoqi|X|', 'Finn|Peter A|PA|', 'Nielsen|Christian B|CB|http://orcid.org/0000-0002-8591-1203', 'Timis|Cozmin|C|', 'Nooney|Tamsin|T|', 'Bevan|Adrian|A|', 'Kreouzis|Theo|T|http://orcid.org/0000-0003-2326-5338']

2021

32783366

Genetic variation in ABCB5 associates with risk of hepatocellular carcinoma.

Expression of ATP-binding cassette B5 (ABCB5) has been demonstrated to confer chemoresistance, enhance cancer stem cell properties and associate with poor prognosis in hepatocellular carcinoma (HCC). The aim of this study was to evaluate the genetic variations of ABCB5 in HCC patients with reference to healthy individuals and the clinicopathological significance. A pilot study has examined 20 out of 300 pairs HCC and paralleled blood samples using conventional sequencing method to cover all exons and exon/intron regions to investigate whether there will be novel variant sequence and mutation event. A total of 300 HCC and 300 healthy blood DNA samples were then examined by Sequenom MassARRAY genotyping and pyrosequencing for 38 SNP and 1 INDEL in ABCB5. Five novel SNPs were identified in ABCB5. Comparison of DNA from blood samples of HCC and healthy demonstrated that ABCB5 SNPs rs75494098, rs4721940 and rs10254317 were associated with HCC risk. Specific ABCB5 variants were associated with aggressive HCC features. SNP rs17143212 was significantly associated with ABCB5 expression level. Nonetheless, the paralleled blood and tumour DNA sequences from HCC patients indicated that ABCB5 mutation in tumours was not common and corroborated the TCGA data sets. In conclusion, ABCB5 genetic variants had significant association with HCC risk and aggressive tumour properties.

['Leung|Idy C-Y|IC|', 'Chong|Charing C-N|CC|', 'Cheung|Tan T|TT|', 'Yeung|Philip C|PC|', 'Ng|Kelvin K-C|KK|', 'Lai|Paul B-S|PB|', 'Chan|Stephen L|SL|', 'Chan|Anthony W-H|AW|', 'Tang|Patrick M-K|PM|0000-0002-3194-3736', 'Cheung|Siu T|ST|']

2020

30596527

Polyethylene glenoid component fixation geometry influences stability in total shoulder arthroplasty.

Glenoid component stability is essential to ensure successful long-term survivability following total shoulder arthroplasty. As such, this computational study assessed the stability of five all-polyethylene glenoid components (Keel, Central-Finned 4-Peg, Peripheral 4-Peg, Cross-Keel, and Inverted-Y), using simulated joint loading in an osteoarthritic patient cohort. Stability was assessed on the basis of component micromotion in the tangential and normal directions. Maximum tangential micromotion occurred in the Cross-Keel (146 ± 46 µm), which was significantly greater (p < .001) than the other components. Maximum normal micromotion occurred in the Inverted-Y (109 ± 43 µm), which was significantly greater (p ≤ .002) than the other four components. In general, the Central-Finned 4-Peg exhibited the least normal and tangential micromotion, while the keeled components shown the highest normal and tangential micromotion. This study suggests that modifications to keeled designs do not improve component stability under the conditions tested, and pegged components show superior computational stability.

['Knowles|Nikolas K|NK|', 'Langohr|G Daniel G|GDG|', 'Athwal|George S|GS|', 'Ferreira|Louis M|LM|']

2019

25955293

Locus of control and coping strategies in older persons with and without depression.

OBJECTIVES To compare locus of control and coping strategies in older persons with and without depression. METHOD This cross-sectional study included 144 depressed in-patients from seven psychogeriatric hospital units, and 106 community-dwelling older persons without depression. All participants were 60 years and older. Locus of control was assessed by a 17-items self-report questionnaire with six response categories. Coping strategies were assessed by a 26-items self-report questionnaire with five response categories. For analytical purposes, age (<75 years vs. ≥75 years), level of education (<10 years vs. ≥10 years) and general medical health (poor vs. not poor) were categorized. RESULTS In linear regression analysis, controlling for demographics, health, and social variables, the depressed in-patients showed a higher external locus of control orientation and a less frequent use of problem-focused coping strategies compared with the non-depressed group. No differences in use of emotion-focused strategies were found between the two groups. CONCLUSION Compared with the non-depressed old persons, the depressed hospitalized older persons were characterized by perceptions of less personal control, and less use of problem-focused strategies, what also might have brought positive alterations into their situation.

['Bjørkløf|Guro Hanevold|GH|', 'Engedal|Knut|K|', 'Selbæk|Geir|G|', 'Maia|Deborah Bezerra|DB|', 'Coutinho|Evandro Silvia Freire|ES|', 'Helvik|Anne-Sofie|AS|']

2016

31497757

Ovarian drilling in polycystic ovary syndrome: Long term pregnancy rate.

Objective To report long term pregnancy rate in polycystic ovary syndrome (PCOS) treated by ovarian drilling. To evaluate predictive factors of pregnancy and possibility of a second drilling. Design Retrospective, observational, multicenter study. Setting Gynecologic departments of two teaching's hospitals. Patients All infertile women with PCOS who were treated by ovarian drilling from 2004 to 2013. The Rotterdam criteria were applied to define PCOS. Interventions Surgical ovarian drilling by laparoscopy and trans vaginal hydro laparoscopy. Main Outcome Measures The primary endpoint was pregnancy rate after ovarian drilling. The secondary endpoints were the predictive factors of pregnancy and the possibility of a second ovarian drilling. Results 289 women were included in the study. The mean follow-up period was 28.4 months (25.3-31.5). A pregnancy was obtained in at least 137 (47.4%) women after a drilling, and 71 (51.8%) of these pregnancies were spontaneous, 48 (16.6%) women achieved at least two pregnancies after drilling, and 27 (56.3%) of these were spontaneous. The predictive factors for effectiveness were a normal body mass index (BMI), an infertility period of less than three years, an AFC of less than 50, and an age of less than 35. Second drillings were performed on 33 women. Among them, 19 (57.6%) achieved at least one pregnancy, and 10 (52.6%) of these were spontaneous. It appeared that a second drilling was effective either when the first drilling had been successful (pregnancy achieved after drilling) or when it had failed in cases of high AFC (greater than 55). Conclusion Ovarian drilling permitted to obtain spontaneous pregnancy for women with PCOS. This surgery could have durably effect permitted to obtain more than one pregnancy.

['Debras|E|E|', 'Fernandez|H|H|', 'Neveu|M-E|ME|', 'Deffieux|X|X|', 'Capmas|P|P|']

2019

30462196

Cardiovascular magnetic resonance can be undertaken in pregnancy and guide clinical decision-making in this patient population.

AIMS Cardiovascular disease is the leading cause of maternal morbidity and mortality, frequently requiring cardiac imaging for diagnosis, and follow-up. This need does not change pregnancy; however, many centres do not offer cardiovascular magnetic resonance (CMR) to pregnant patients. This paper explores current practice of CMR in pregnancy in four large volume centres, its safety and its impact on patient management. METHODS AND RESULTS Between 2008 and 2017, we collected consecutive pregnant patients between four centres. Guidelines and local standard operating procedures were followed and outcomes recorded modelled on the EuroCMR registry. Eighty-three women had diagnostic CMR without immediate complications. The commonest indication was vascular or congenital disease (48%), followed by cardiomyopathy/myocarditis (43%). Nineteen percent received contrast, and CMR changed management in 35% and in 50% of patients who received contrast. CONCLUSION In this largest cohort to date describing CMR in pregnancy, we found that results frequently change management, thus adding valuable guidance for patient care. We conclude that CMR should be offered to pregnant women when indicated, including the administration of contrast as per current guidelines.

['Herrey|Anna S|AS|', 'Francis|Jane M|JM|', 'Hughes|Marina|M|', 'Ntusi|Ntobeko A B|NAB|']

2019

24209888

Factors associated with calcium absorption in postmenopausal women: a post hoc analysis of dual-isotope studies.

Reduced calcium absorption is a risk factor for osteoporosis. This study examined factors associated with fractional calcium absorption (FCA) and net calcium absorption in postmenopausal women in a post hoc analysis of three completed dual-isotope studies. Data were analyzed from 50 postmenopausal women undergoing 121 inpatient research visits in three studies evaluating changes in FCA related to correction of vitamin D insufficiency (n=19), use of proton pump inhibitors (n=21), and use of aromatase inhibitors to treat breast cancer (n=10). Net calcium absorption was the product of FCA and total calcium intake in milligrams per day. Variables included subjects' age, race, body mass index, serum calcium, creatinine, parathyroid hormone, 1,25-dihydroxyvitamin D, 25-hydroxyvitamin D, and habitual intake of kilocalories, protein, fat, carbohydrate, fiber, calcium, iron, magnesium, oxalate, phosphorus, potassium, and vitamin D based on outpatient diet diaries. In multivariate models, subjects' age, dietary intake of kilocalories, carbohydrates, fat, fiber, calcium, and potassium were significant predictors of FCA. In multiple variable models predicting net calcium absorption, dietary intake of kilocalories, fat, fiber, calcium, potassium, and serum 1,25-dihydroxyvitamin D were significant. The square of the correlation between actual and predicted values (an approximation of R(2)) was 0.748 for FCA and 0.726 for net calcium absorption. Similar to other studies, this study found that age, 1,25-dihydroxyvitamin D, and dietary calcium and fat were associated with calcium absorption. Dietary intake of kilocalories, carbohydrates, and potassium were new factors that were significantly associated with FCA and net calcium absorption. In summary, the study suggests that several dietary habits play a role in calcium absorption, beyond vitamin D and calcium.

['Ramsubeik|Karishma|K|', 'Keuler|Nicholas S|NS|', 'Davis|Lisa A|LA|', 'Hansen|Karen E|KE|']

2014

27988092

Safety of Tranexamic Acid in Pediatric Cardiac Surgery: A Nationwide Database Study.

OBJECTIVES The present study aimed to examine the association between tranexamic acid (TXA) use and adverse effects (seizures, thromboembolism, and renal dysfunction) in a pediatric cardiac surgery population using a national inpatient database in Japan. The authors also assessed the association between TXA use and other clinical outcomes (length of hospital stay and in-hospital mortality). DESIGN A nationwide, retrospective cohort study using propensity score analyses. SETTING Japanese Diagnosis Procedure Combination inpatient database. PARTICIPANTS Pediatric patients who underwent cardiac surgery using cardiopulmonary bypass between July 2010 and March 2014 (N = 11,275). INTERVENTIONS None. MEASUREMENTS AND MAIN RESULTS Propensity-score matching created 3,739 pairs of patients with and without TXA administration. Propensity-matched analysis showed that the proportion of seizures was significantly higher in the TXA group than in the non-TXA group (1.6% v 0.2%, difference, 1.4%; 95% confidence interval, 1.0-1.9; p<0.001). However, none of the other outcomes was significantly different between the groups. CONCLUSIONS TXA use is associated with a significantly increased risk of seizures. However, there is no difference in any other outcomes between the TXA and non-TXA groups.

['Maeda|Takuma|T|', 'Sasabuchi|Yusuke|Y|', 'Matsui|Hiroki|H|', 'Ohnishi|Yoshihiko|Y|', 'Miyata|Shigeki|S|', 'Yasunaga|Hideo|H|']

2017

31223014

Does the new vascular management of acute limb ischemia have effective results with lower treatment costs.

Aim To compare hospital costs of acute limb ischemia treatment in two periods of time and to show evidence of long-term repercussions on reducing costs during successful treatment. Methods Retrospective analysis of data obtained from 100 patients' medical history in the period 2000-2016 at the Clinic of Vascular Surgery Sarajevo: group A - 60 patients with acute limb ischemia in the period 2005-2016 and group B - 40 patients with acute limb ischemia (ALI) in the period 2000-2005. From 2000 to 2005 conservative treatment method was used, invasive diagnostic and surgical procedures were often delayed for a shorter or longer period of time. During the period from 2005 to 2016, the management model and safe practice included emergency diagnostic procedures, colour-Doppler, arteriography, emergency surgery (embolectomy by Fogharty and if necessary, vascular by-pass). Results Better health service for the patients with acute limb ischemia was offered in the period 2005-2016, which relied on proven medical treatment trends. The largest share of the total costs of each patient included costs of hospital bed with significant difference between the period 2005-2016 and 2000-2005, mean of 1398.71 KM and 2480.45KM, respectively (p<0.0001), indicating rationalization of time that patients spend at the Vascular Clinic. Conclusion This trend of money/fund savings is an example of good practice, effectiveness and efficiency in the treatment of ALI and as such was used in patients with other vascular diseases.

['Vukas|Haris|H|', 'Kadić-Vukas|Samra|S|', 'Dervišević|Senad|S|', 'Salihbegović|Adis|A|', 'Vranić|Haris|H|', 'Djedović|Muhamed|M|', 'Hadžimehmedagić|Amel|A|', 'Totić|Dragan|D|']

2019

33160021

β-hydroxybutyrate (β-HB) exerts anti-inflammatory and antioxidant effects in lipopolysaccharide (LPS)-stimulated macrophages in Liza haematocheila.

Poly-β-hydroxybutyrate (PHB) can be hydrolyzed to β-hydroxybutyrate (β-HB) in the intestinal tract of animals, and dietary PHB supplementation could enhance the immunity and disease resistance of aquatic animals. Antioxidant system is responsive to PHB stimuli via MAPK/PI3K-Akt/TNF/NF-κB/TCR/TLR signaling pathways. However, the precise immunopotentiation mechanism needs further study. In this study, macrophages from spleen in Liza haematocheila was used to study the effect of β-HB on cell viability and antioxidant function to illustrate the immunopotentiation mechanism of PHB. The results showed that β-HB (100 μg/mL) promoted the viability of macrophages and balanced the production of reactive oxygen species, but inhibited the excessive production of intracellular nitric oxide. In order to further explore the immunopotentiation mechanism of β-HB, LPS (100 μg/mL) was used to induce the inflammation and investigated the inhibitory effect of β-HB on inflammation. The results showed that LPS could induce inflammation successfully, and β-HB exerted anti-inflammatory and antioxidant effects in LPS-stimulated macrophages. Compared with LPS stimuli alone, the expression of anti-inflammatory genes NF-κBIA, MAP3K8 and TLR5 in β-HB pretreatment group was up-regulated, and the expression of pro-inflammatory genes TNFSF6, TNF-α, PI3K, NF-κB and TLR1 down-regulated. It suggested that β-HB inhibited the inflammatory response by up-regulation of anti-inflammatory genes such as NF-κBIA, thereby enhancing the immunity of the body.

['Qiao|Guo|G|', 'Lv|Tingli|T|', 'Zhang|Mingming|M|', 'Chen|Peng|P|', 'Sun|Qirui|Q|', 'Zhang|Jialin|J|', 'Li|Qiang|Q|']

2020

28365113

Associations between pathogen-specific cases of subclinical mastitis and milk yield, quality, protein composition, and cheese-making traits in dairy cows.

The aim of this study was to investigate associations between pathogen-specific cases of subclinical mastitis and milk yield, quality, protein composition, and cheese-making traits. Forty-one multibreed herds were selected for the study, and composite milk samples were collected from 1,508 cows belonging to 3 specialized dairy breeds (Holstein Friesian, Brown Swiss, and Jersey) and 3 dual-purpose breeds of Alpine origin (Simmental, Rendena, and Grey Alpine). Milk composition [i.e., fat, protein, casein, lactose, pH, urea, and somatic cell count (SCC)] was analyzed, and separation of protein fractions was performed by reversed-phase high performance liquid chromatography. Eleven coagulation traits were measured: 5 traditional milk coagulation properties [time from rennet addition to milk gelation (RCT, min), curd-firming rate as the time to a curd firmness (CF) of 20 mm (k20, min), and CF at 30, 45, and 60 min from rennet addition (a30, a45, and a60, mm)], and 6 new curd firming and syneresis traits [potential asymptotical CF at an infinite time (CFP, mm), curd-firming instant rate constant (kCF, % × min-1), curd syneresis instant rate constant (kSR, % × min-1), modeled RCT (RCTeq, min), maximum CF value (CFmax, mm), and time at CFmax (tmax, min)]. We also measured 3 cheese yield traits, expressing the weights of total fresh curd (%CYCURD), dry matter (%CYSOLIDS), and water (%CYWATER) in the curd as percentages of the weight of the processed milk, and 4 nutrient recovery traits (RECPROTEIN, RECFAT, RECSOLIDS, and RECENERGY), representing the percentage ratio between each nutrient in the curd and milk. Milk samples with SCC > 100,000 cells/mL were subjected to bacteriological examination. All samples were divided into 7 clusters of udder health (UH) status: healthy (cows with milk SCC < 100,000 cells/mL and uncultured); culture-negative samples with low, medium, or high SCC; and culture-positive samples divided into contagious, environmental, and opportunistic intramammary infection (IMI). Data were analyzed using a linear mixed model. Significant variations in the casein to protein ratio and lactose content were observed in all culture-positive samples and in culture-negative samples with medium to high SCC compared to normal milk. No differences were observed among contagious, environmental, and opportunistic pathogens, suggesting an effect of inflammation rather than infection. The greatest impairment in milk quantity and composition, clotting ability, and cheese production was observed in the 2 UH status groups with the highest milk SCC (i.e., contagious IMI and culture-negative samples with high SCC), revealing a discrepancy between the bacteriological results and inflammatory status, and thus confirming the importance of SCC as an indicator of udder health and milk quality.

['Bobbo|T|T|', 'Ruegg|P L|PL|', 'Stocco|G|G|', 'Fiore|E|E|', 'Gianesella|M|M|', 'Morgante|M|M|', 'Pasotto|D|D|', 'Bittante|G|G|', 'Cecchinato|A|A|']

2017

26696259

Navigating Barriers to Vocational Rehabilitation for HIV-Positive Persons.

This study documented the outcomes of 108 HIV-positive persons receiving vocational rehabilitation services. Over a 12-month follow-up, participants reported significantly decreased odds of any unstable housing [Adjusted Odds Ratio (AOR) = 0.21; 95 % CI 0.05-0.90; p < .05] and increased odds of being employed at least part-time (AOR = 10.19; 95 % CI 2.40-43.21; p < .01). However, reductions in perceived barriers to employment and increases in income were more pronounced among those not receiving disability benefits at baseline. This was consistent with findings from baseline qualitative interviews with 22 participants where those not on disability were subject to bureaucratic hurdles to rapidly accessing benefits and anticipated stigma of being on disability that propelled them to rejoin the workforce. Vocational rehabilitation could address key structural barriers to optimizing HIV treatment as prevention, and novel approaches are needed to improve outcomes among individuals receiving disability benefits.

['Gómez|Walter|W|', 'Flentje|Annesa|A|', 'Schustack|Amy|A|', 'Ramirez-Forcier|Joseph|J|', 'Andrews|Brett|B|', 'Dilworth|Samantha E|SE|', 'Riley|Elise D|ED|', 'Curotto|Alberto|A|', 'Carrico|Adam W|AW|']

2016

34452391

Detection and Molecular Characterization of Two Gammaherpesviruses from Pantesco Breed Donkeys during an Outbreak of Mild Respiratory Disease.

Equid and asinine gammaherpesviruses (GHVs; genus Percavirus) are members of the Herpesviridae family. Though GHVs have been reported in horse populations, less studies are available on gammaherpesviral infections in donkeys. This study reports the co-infection with two GHVs in Pantesco breed donkeys, an endangered Italian donkey breed. Samples (n = 124) were collected on a breeding farm in Southern Italy from 40 donkeys, some of which were healthy or presented erosive tongue lesions and/or mild respiratory signs. Samples were analysed by using a set of nested PCRs targeting the DNA polymerase, glycoprotein B, and DNA-packaging protein genes, and sequence and phylogenetic analyses were performed. Twenty-nine donkeys (72.5%) tested positive, and the presence of Equid gammaherpesvirus 7 and asinine herpesvirus 5 was evidenced. In 11 animals, we found evidence for co-infection with viruses from the two species. Virions with herpesvirus-like morphology were observed by electron microscopic examination, and viruses were successfully isolated in RK-13-KY cell monolayers. The histological evaluation of tongue lesions revealed moderate lympho-granulocytic infiltrates and rare eosinophilic inclusions. The detection of GHVs in this endangered asinine breed suggests the need long-life monitoring within conservation programs and reinforces the need for further investigations of GHV's pathogenetic role in asinine species.

['Mira|Francesco|F|0000-0003-1276-0579', 'Canuti|Marta|M|0000-0002-9959-128X', 'Di Bella|Santina|S|0000-0002-6766-9126', 'Puleio|Roberto|R|0000-0002-3461-3353', 'Lavazza|Antonio|A|0000-0002-7646-3592', 'Lelli|Davide|D|0000-0002-5775-6058', 'Vicari|Domenico|D|0000-0002-8902-6383', 'Purpari|Giuseppa|G|0000-0001-7285-5321', 'Cannella|Vincenza|V|0000-0001-5768-5782', 'Chiaramonte|Gabriele|G|', 'Schirò|Giorgia|G|0000-0003-3323-6653', 'Castronovo|Calogero|C|', 'Guercio|Annalisa|A|']

2021

23816459

3D brain tumor segmentation in multimodal MR images based on learning population- and patient-specific feature sets.

Brain tumor segmentation is a clinical requirement for brain tumor diagnosis and radiotherapy planning. Automating this process is a challenging task due to the high diversity in appearance of tumor tissue among different patients and the ambiguous boundaries of lesions. In this paper, we propose a method to construct a graph by learning the population- and patient-specific feature sets of multimodal magnetic resonance (MR) images and by utilizing the graph-cut to achieve a final segmentation. The probabilities of each pixel that belongs to the foreground (tumor) and the background are estimated by global and custom classifiers that are trained through learning population- and patient-specific feature sets, respectively. The proposed method is evaluated using 23 glioma image sequences, and the segmentation results are compared with other approaches. The encouraging evaluation results obtained, i.e., DSC (84.5%), Jaccard (74.1%), sensitivity (87.2%), and specificity (83.1%), show that the proposed method can effectively make use of both population- and patient-specific information.

['Jiang|Jun|J|', 'Wu|Yao|Y|', 'Huang|Meiyan|M|', 'Yang|Wei|W|', 'Chen|Wufan|W|', 'Feng|Qianjin|Q|']

2013

27068109

Fear-of-intimacy-mediated zinc transport controls the function of zinc-finger transcription factors involved in myogenesis.

Zinc is a component of one-tenth of all human proteins. Its cellular concentration is tightly regulated because its dyshomeostasis has catastrophic health consequences. Two families of zinc transporters control zinc homeostasis in organisms, but there is little information about their specific developmental roles. We show that the ZIP transporter Fear-of-intimacy (Foi) is necessary for the formation of Drosophila muscles. In foi mutants, myoblasts segregate normally, but their specification is affected, leading to the formation of a misshapen muscle pattern and distorted midgut. The observed phenotypes could be ascribed to the inactivation of specific zinc-finger transcription factors (ZFTFs), supporting the hypothesis that they are a consequence of intracellular depletion of zinc. Accordingly, foi phenotypes can be rescued by mesodermal expression of other ZIP members with similar subcellular localization. We propose that Foi acts mostly as a transporter to regulate zinc intracellular homeostasis, thereby impacting on the activity of ZFTFs that control specific developmental processes. Our results additionally suggest a possible explanation for the presence of large numbers of zinc transporters in organisms based on differences in ion transport specificity and/or degrees of activity among transporters.

['Carrasco-Rando|Marta|M|', 'Atienza-Manuel|Alexandra|A|', 'Martín|Paloma|P|', 'Burke|Richard|R|', 'Ruiz-Gómez|Mar|M|0000-0002-9024-1400']

2016