Transferring the exudate in the tissue engineering chamber as a trigger to incubate large amount adipose tissue in remote area.
Tissue engineering chamber (TEC) is a technique that could incubate up to 16 folds volume increase of a fat flap. But the mechanism in the silicone chamber was still unknown. The function of exudate in the chamber was noticed recently. We developed a special model called fluid drainage model (FDM) that consisted of a traditional TEC on the back and paired fat flaps without a chamber in the groins. Then we used a silicon tube to dynamically transfer the exudate from dorsal TEC to one of the paired inguinal fat flap and the other inguinal fat flap with a sham tube was set as control. At Week 4, the volume of drainage group reached 8.7 ± 2.3 ml, 576 ± 152% to its original volume whereas the growth ratio of control group was only 130 ± 39%. Similar volume change and histological change were observed within fat flap from TEC model and drainage group. The exudate in the TEC is a heterogeneous cocktail contains cytokines as well as cells. Intriguingly, transferred exudate in the TEC model sustain the ability to incubate large amount of adipose tissue remotely.
['Lei|Chen|C|0000-0002-7198-6482', 'Dong|Ziqing|Z|', 'Wan|Jinlin|J|', 'Xiao|Xiaolian|X|', 'Lu|Feng|F|', 'Wang|Biao|B|']
D000273:Adipose Tissue D000818:Animals D016207:Cytokines D004322:Drainage D005122:Exudates and Transudates D005260:Female D021621:Imaging, Three-Dimensional D019394:Ki-67 Antigen D018919:Neovascularization, Physiologic D011817:Rabbits D013524:Surgical Flaps D023822:Tissue Engineering D055114:X-Ray Microtomography
tissue engineering chamber adipose tissue fat flap fluid drainage model exudate transferring