Novel miRNA-based biomarker panel for detection β2-agonists in goats.
miRNA sequencing was applied in this work to screen miRNA biomarkers related to β2-agonists from the test and control goat samples. A total of 10 selected miRNAs were proven by qRT-PCR to be able to separate treatment cell groups from the control. With previously reported differentially expressed genes (DEGs), we used target gene prediction to build a miRNA-mRNA regulatory network related to β2-agonists, which validated the miRNA biomarkers and provided a reference for identifying the mechanism of β2-agonists. Our subsequent in vivo experiments revealed that the regulation trends of the miRNAs were the same as in vitro experiments. DD-SIMCA and heatmap analysis also indicated concordant separation effects with the 10 miRNAs, which could therefore be used as biomarkers to monitor illegal use of β2-agonists in goats.
['Zhao|Luyao|L|', 'Yang|Shuming|S|', 'You|Xinyong|X|', 'He|Wenjing|W|', 'Xue|Jiali|J|']
D058666:Adrenergic beta-2 Receptor Agonists D000818:Animals D015415:Biomarkers D005786:Gene Expression Regulation D053263:Gene Regulatory Networks D006041:Goats D006801:Humans D008297:Male D035683:MicroRNAs D012333:RNA, Messenger
Biomarkers miRNA sequencing Mechanism Abuse β(2)-agonists