Molecular characterization of Kiss2 receptor and in vitro effects of Kiss2 on reproduction-related gene expression in the hypothalamus of half-smooth tongue sole (Cynoglossus semilaevis).
Kisspeptin (Kiss) and its receptor, KissR (previously known as GPR54), play a critical role in the control of reproduction and puberty onset in mammals. Additionally, a number of studies have provided evidence of the existence of multiple Kiss/KissR systems in teleosts, but the physiological relevance and functions of these kisspeptin forms (Kiss1 and Kiss2) still remain to be investigated. To this end, we examined the direct actions of Kiss2 on hypothalamic functions in the half-smooth tongue sole (Cynoglossus semilaevis), a representative species of the order Pleuronectiformes. As a first step, the full-length cDNA for kiss2r was identified and kiss2r transcripts were shown to be widely expressed in various tissues, notably in the brain of tongue sole. Then, the effects of Kiss2 decapeptide on reproduction-related gene expression were evaluated using a primary hypothalamus culture system. Our results showed that neither gnrh2 nor gnrh3 mRNA levels were altered by Kiss2. However, Kiss2 significantly increased the amounts of gnih and kiss2 mRNAs. In contrast, Kiss2 elicited an evident inhibitory effect on both gnihr and kiss2r mRNA levels. To the best of our knowledge, this is the first description of a direct and differential regulation of reproduction-related gene expression by Kiss2 at the hypothalamus level of a teleost fish. Overall, this study provides novel information on the role of Kiss2/Kiss2R system in the reproductive function of teleosts.
['Wang|Bin|B|', 'Liu|Quan|Q|', 'Liu|Xuezhou|X|', 'Xu|Yongjiang|Y|', 'Shi|Bao|B|']
D000595:Amino Acid Sequence D000818:Animals D001483:Base Sequence D003001:Cloning, Molecular D018076:DNA, Complementary D005260:Female D005412:Flatfishes D020869:Gene Expression Profiling D005786:Gene Expression Regulation D007031:Hypothalamus D059648:Kisspeptins D010802:Phylogeny D012333:RNA, Messenger D011956:Receptors, Cell Surface D012098:Reproduction D016415:Sequence Alignment D012741:Sexual Maturation
Hypothalamus Cynoglossus semilaevis Kisspeptin Kisspeptin receptor cDNA cloning