A Polyphenolic Complex Attenuates Inflammatory Response and Blood- Brain Barrier Disruption.
BACKGROUND
Cerebral ischemia causes a strong inflammatory response. Neumentix is a dietary supplement containing 14.9% rosmarinic acid and 29.9% total phenolic content, which has been proved to be beneficial against inflammatory response. Therefore, Neumentix's effect on anti-inflammatory and blood brain barrier (BBB) disruption in transient middle cerebral artery occlusion (tMCAO) model mice is investigated in this study.


METHODS
After the pretreatment of vehicle or Neumentix 134 mg/kg/d, intraperitoneal injection (i.p.) (containing rosmarinic acid 20 mg/kg/d) for 14 days, mice were subjected to tMCAO for 60 min and kept receiving vehicle or Neumentix daily 5 days afterward.


RESULTS
Neumentix treatment ameliorated neurobehavioral impairment in the corner test (5d after tMCAO, **P<0.01), reduced infarct volume (#P<0.05), suppressed expression of ionized calciumbinding adapter molecule-1 (Iba-1), tumor necrosis factor alpha (TNF-α) and monocyte chemoattractant protein-1 (MCP-1) (###P<0.001), and improved the integrity of BBB (§P<0.05) at 5 days after tMCAO.


CONCLUSION
The present study provided an evidence of Neumentix's anti-inflammatory and neuroprotection effect against BBB disruption on experimental tMCAO model mice, suggesting that Neumentix could be a potential therapeutic agent for stroke.
['Bian|Yuting|Y|', 'Yamashita|Toru|T|', 'Taira|Yuki|Y|', 'Shang|Jingwei|J|', 'Tsunoda|Keiichiro|K|', 'Feng|Tian|T|', 'Sasaki|Ryo|R|', 'Liu|Xia|X|', 'Shi|Xiaowen|X|', 'Tadokoro|Koh|K|', 'Nomura|Emi|E|', 'Matsumoto|Namiko|N|', 'Osakada|Yusuke|Y|', 'Omote|Yoshio|Y|', 'Takemoto|Mami|M|', 'Hishikawa|Nozomi|N|', 'Ohta|Yasuyuki|Y|', 'Abe|Koji|K|']
D000818:Animals D000893:Anti-Inflammatory Agents D001812:Blood-Brain Barrier D002934:Cinnamates D053630:Depsides D019587:Dietary Supplements D020244:Infarction, Middle Cerebral Artery D018836:Inflammation Mediators D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D018696:Neuroprotective Agents D059808:Polyphenols
polyphenolic complex rosmarinic acid middle cerebral artery occlusion Ischemic stroke anti-inflammation blood
brain barrier