Actinoplanes deserti sp. nov., isolated from a desert soil sample.
A novel actinomycete, designated strain YIM CF22T, was isolated from a desert soil sample collected from Turpan in Xinjiang Uyghur Autonomous Region, north-western China. The taxonomic position of the strain YIM CF22T is described based on a polyphasic approach. Strain YIM CF22T was found to form irregular sporangia on agar media. It contains meso-diaminopimelic acid in the cell wall peptidoglycan. The major menaquinone was identified as MK-9(H4); the polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified glycolipids. The whole cell sugars were found to be ribose, mannose, galactose, glucose and xylose. The major cellular fatty acids were found to be (> 5%) iso-C16:0 (43.5%), anteiso-C17:0 (10.2%), iso-C15:0 (7.1%), C17:1 ω8c (6.3%) and iso H-C16:1 (5.9%). The G+C content was determined to be 70.8%. 16S rRNA gene sequence analysis of strain YIM CF22T showed high similarity (97.0%) to Actinoplanes rishiriensis NBRC 108556T. The strain also showed high 16S rRNA gene sequence similarities to Verrucosispora sediminis CGMCC 4.3550T (96.9%) and Micromonospora tulbaghiae DSM 45142T (96.8%). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM CF22T clusters with A. rishiriensis NBRC 108556T, Actinoplanes globisporus JCM 3186T and Actinoplanes rhizophilus NEAU-A-2T. Based on the differential phenotypic characteristics and the results of DNA-DNA relatedness and phylogenetic analysis, it is proposed that strain YIM CF22T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes deserti sp. nov. is proposed. The type strain is YIM CF22T (= KCTC 39543T = CCTCC AB2018113T).
['Habib|Neeli|N|', 'Khan|Inam Ullah|IU|', 'Chu|Xiao|X|', 'Xiao|Min|M|', 'Li|Shuai|S|', 'Fang|Bao-Zhu|BZ|', 'Zhi|Xiao-Yang|XY|', 'Li|Wen-Jun|WJ|http://orcid.org/0000-0002-1233-736X']
D001482:Base Composition D002473:Cell Wall D002681:China D003889:Desert Climate D003960:Diaminopimelic Acid D005227:Fatty Acids D005690:Galactose D005947:Glucose D006017:Glycolipids D008358:Mannose D008848:Micromonosporaceae D010457:Peptidoglycan D010743:Phospholipids D010802:Phylogeny D012336:RNA, Ribosomal, 16S D012266:Ribose D012988:Soil Microbiology D013171:Spores, Bacterial D024482:Vitamin K 2 D014994:Xylose
Actinoplanes deserti sp. nov. Desert soil Polyphasic taxonomy Turpan desert Xinjiang