Distribution of tumor-infiltrating immune cells in glioblastoma.
AIM
Evaluation of features related to infiltrating immune cell level in glioblastoma.


METHODS
Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis.


RESULTS
CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4+ was associated with unmethylated MGMT (p = 0.016). Higher CD8+ was associated with larger tumoral size (p = 0.027). Higher CD163+ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4+ (p < 0.05) were associated with longer overall survival.


CONCLUSION
Macrophages are more frequent than TILs. Some subsets are associated with clinical features.
['Orrego|Enrique|E|', 'Castaneda|Carlos A|CA|', 'Castillo|Miluska|M|', 'Bernabe|Luis A|LA|', 'Casavilca|Sandro|S|', 'Chakravarti|Arnab|A|', 'Meng|Wei|W|', 'Garcia-Corrochano|Pamela|P|', 'Villa-Robles|Maria R|MR|', 'Zevallos|Rocio|R|', 'Mejia|Omar|O|', 'Deza|Pedro|P|', 'Belmar-Lopez|Carolina|C|', 'Ojeda|Luis|L|']
D000293:Adolescent D000328:Adult D000368:Aged D014408:Biomarkers, Tumor D001932:Brain Neoplasms D002648:Child D015331:Cohort Studies D019175:DNA Methylation D015254:DNA Modification Methylases D045643:DNA Repair Enzymes D005260:Female D005909:Glioblastoma D006801:Humans D016246:Lymphocytes, Tumor-Infiltrating D008264:Macrophages D008297:Male D008875:Middle Aged D011401:Promoter Regions, Genetic D025521:Tumor Suppressor Proteins D055815:Young Adult
glioblastoma tumor-infiltrating lymphocytes macrophages MGMT overall survival biomarker prognosis