60-kDa Tat-interactive protein (TIP60) positively regulates Th-inducing POK (ThPOK)-mediated repression of eomesodermin in human CD4+ T cells.
The abundant expression of IFNγ in Th-inducing POK (ThPOK)-deficient CD4(+) T cells requires the activation of Eomesodermin (Eomes); however, the underlying mechanism of this phenomenon remains unclear. Here we report that ThPOK binds directly to the promoter region of the Eomes gene to repress its expression in CD4(+) T cells. We identified the histone acetyltransferase TIP60 as a co-repressor of ThPOK-target genes, where ectopically expressed TIP60 increased ThPOK protein stability by promoting its acetylation at its Lys(360) residue to then augment the transcriptional repression of Eomes. Moreover, knockdown of endogenous TIP60 abolished the stabilization of ThPOK in CD4(+) T cells, which led to the transcriptional activation of Eomes and increased production of IFNγ. Our results reveal a novel pathway by which TIP60 and ThPOK synergistically suppresses Eomes function and IFNγ production, which could contribute to the regulation of inflammation.
['Li|Yangyang|Y|', 'Tsun|Andy|A|', 'Gao|Zhimei|Z|', 'Han|Zhijun|Z|', 'Gao|Yayi|Y|', 'Li|Zhiyuan|Z|', 'Lin|Fang|F|', 'Wang|Yan|Y|', 'Wei|Gang|G|', 'Yao|Zhengju|Z|', 'Li|Bin|B|']
D000107:Acetylation D015496:CD4-Positive T-Lymphocytes D004268:DNA-Binding Proteins D005786:Gene Expression Regulation D057809:HEK293 Cells D051548:Histone Acetyltransferases D006801:Humans D007249:Inflammation D007371:Interferon-gamma D000074443:Lysine Acetyltransferase 5 D011401:Promoter Regions, Genetic D055550:Protein Stability D012097:Repressor Proteins D020825:T-Box Domain Proteins D014157:Transcription Factors
TIP60 Eomesodermin T Cell ThPOK Protein Stability Acetylation Inflammation Histone Acetylase Transcription Regulation