Acyclovir inhibits channel catfish virus replication and protects channel catfish ovary cells from apoptosis.
The channel catfish virus (CCV) can cause lethal hemorrhagic infection in channel catfish, resulting in significant economic losses in the fish industry. Effective drugs for the virus are still lacking. Acyclovir is known as a potent antiviral agent against human herpes viruses and some animal DNA viruses. The present study was undertaken to explore the antiviral response and mechanism of acyclovir against CCV in channel catfish ovary (CCO) cells. Acyclovir was able to significantly inhibit the expression of viral genes related to CCV viral DNA synthesis and suppress viral replication at a safe concentration. Furthermore, acyclovir blocked the cytopathic effects and apoptosis induced by CCV, thereby maintaining the normal cellular morphological structure, as shown by the protection of CCO cells from the formation of apoptotic bodies or nuclear fragmentation. Moreover, reverse transcript quantitative polymerase chain reaction (RT-qPCR) demonstrated that acyclovir suppressed the expression of caspase 3, caspase 8 and caspase 9, while there was no significant impact on the expression of the apoptosis-inhibiting gene bcl-2 in CCV-infected cells. In addition, acyclovir did not promote the expression of immune-related genes such as MyD88, Mx1, IRF3, IRF7, IFN-I, NF-kB and IL-1β, suggesting that the antiviral activity of acyclovir to CCV infection is not achieved by facilitating the expression of immune-related genes in CCO cells. Taken together, the results from this study suggest that acyclovir could effectively regulate CCV-induced infection, and thus is a promising therapeutic agent against CCV. Our results will aid our understanding of the pharmacological mechanisms of antiviral agents.
['Hao|Kai|K|', 'Yuan|Sheng|S|', 'Yu|Fei|F|', 'Chen|Xiao Hui|XH|', 'Bian|Wen Ji|WJ|', 'Feng|Yong Hui|YH|', 'Zhao|Zhe|Z|']
D000212:Acyclovir D000818:Animals D000998:Antiviral Agents D017209:Apoptosis D002397:Catfishes D003588:Cytopathogenic Effect, Viral D005260:Female D005393:Fish Diseases D029941:Fish Proteins D015967:Gene Expression Regulation, Viral D052656:Ictalurivirus D010053:Ovary D014764:Viral Proteins D014779:Virus Replication
Acyclovir Channel catfish virus Virus replication Apoptosis Antiviral