New Factors Enhancing the Reactivity of Cysteines in Molten Globule-Like Structures.
Protein cysteines often play crucial functional and structural roles, so they are emerging targets to design covalent thiol ligands that are able to modulate enzyme or protein functions. Some of these residues, especially those involved in enzyme mechanisms-including nucleophilic and reductive catalysis and thiol-disulfide exchange-display unusual hyper-reactivity; such a property is expected to result from a low pKa and from a great accessibility to a given reagent. New findings and previous evidence clearly indicate that pKa perturbations can only produce two-four-times increased reactivity at physiological pH values, far from the hundred and even thousand-times kinetic enhancements observed for some protein cysteines. The data from the molten globule-like structures of ribonuclease, lysozyme, bovine serum albumin and chymotrypsinogen identified new speeding agents, i.e., hydrophobic/electrostatic interactions and productive complex formations involving the protein and thiol reagent, which were able to confer exceptional reactivity to structural cysteines which were only intended to form disulfides. This study, for the first time, evaluates quantitatively the different contributions of pKa and other factors to the overall reactivity. These findings may help to clarify the mechanisms that allow a rapid disulfide formation during the oxidative folding of many proteins.
['Gambardella|Giorgia|G|', 'Cattani|Giada|G|', 'Bocedi|Alessio|A|', 'Ricci|Giorgio|G|']
D003545:Cysteine D004220:Disulfides D006863:Hydrogen-Ion Concentration D007700:Kinetics D009113:Muramidase D010084:Oxidation-Reduction D011487:Protein Conformation D017510:Protein Folding
ribonuclease lysozyme bovine serum albumin chymotrypsinogen oxidative folding chemical kinetics cysteine reactivity hydrophobic interaction molten globule transient complex