A dual-analytes responsive fluorescent probe for discriminative detection of ClO- and N2H4 in living cells.
Hydrazine (N2H4) and ClO- are very harmful for public health, hence it is important and necessary to monitor them in living cells. Herein, we rationally designed and synthesized a dual-analytes responsive fluorescent sensor PTMQ for distinguishing detection of N2H4 and ClO-. PTMQ underwent N2H4-induced double bond cleavage, affording colorimetric and green fluorescence enhancement with good selectivity and a low detection limit (89nM). On the other hand, PTMQ underwent ClO--induced sulfur oxidation and displayed red fluorescence lighting-up response towards ClO- with good selectivity, rapid response (<0.2min) and a low detection limit (58nM). Moreover, PTMQ was successfully employed for in-situ imaging of N2H4 and ClO- in living cells.
['Zhu|Beitong|B|', 'Wu|Xiaoli|X|', 'Rodrigues|João|J|', 'Hu|Xichao|X|', 'Sheng|Ruilong|R|', 'Bao|Guang-Ming|GM|']
D003124:Colorimetry D005456:Fluorescent Dyes D006367:HeLa Cells D006801:Humans D057230:Limit of Detection D013050:Spectrometry, Fluorescence
Dual-analytes Fluorescent probe Living cells Bioimaging ClO(−) N(2)H(4)