Intracellular RNA-tracking methods.
RNA tracking allows researchers to visualize RNA molecules in cells and tissues, providing important spatio-temporal information regarding RNA dynamics and function. Methods such as fluorescent in situ hybridization (FISH) and molecular beacons rely on complementary oligonucleotides to label and view endogenous transcripts. Other methods create artificial chimeric transcripts coupled with bacteriophage-derived coat proteins (e.g. MS2, λN) to tag molecules in live cells. In other approaches, endogenous RNAs are recognized by complementary RNAs complexed with noncatalytic Cas proteins. Each technique has its own set of strengths and limitations that must be considered when planning an experiment. Here, we discuss the mechanisms, advantages, and weaknesses of in situ hybridization, molecular beacons, MS2 tagging and Cas-derived systems, as well as how RNA tracking can be employed to study various aspects of molecular biology.
['George|Logan|L|', 'Indig|Fred E|FE|', 'Abdelmohsen|Kotb|K|', 'Gorospe|Myriam|M|0000-0001-5439-3434']
D052157:Aptamers, Nucleotide D001435:Bacteriophages D000076987:CRISPR-Associated Protein 9 D064113:CRISPR-Cas Systems D045744:Cell Line, Tumor D006801:Humans D017404:In Situ Hybridization, Fluorescence D012333:RNA, Messenger D022661:RNA, Untranslated D017423:Sequence Analysis, RNA D059010:Single-Cell Analysis
RNA labelling mRNAs noncoding RNAs post-transcriptional gene regulation ribonucleoprotein complexes