Transcriptomic and epigenomics atlas of myotubes reveals insight into the circadian control of metabolism and development.
Aim: Innate circadian rhythms are critical for optimal tissue-specific functions, including skeletal muscle, a major insulin-sensitive tissue responsible for glucose homeostasis. We determined whether transcriptional oscillations are associated with CpG methylation changes in skeletal muscle. Materials & methods: We performed rhythmicity analysis on the transcriptome and CpG methylome of circadian synchronized myotubes. Results: We identified several transcripts and CpG-sites displaying oscillatory behavior, which were enriched with Gene Ontology terms related to metabolism and development. Oscillating CpG methylation was associated with rhythmic expression of 31 transcripts. Conclusion: Although circadian oscillations may be regulated by rhythmic DNA methylation, strong rhythmic associations between transcriptome and CpG methylation were not identified. This resource constitutes a transcriptomic/epigenomic atlas of skeletal muscle and regulation of circadian rhythms.
['Altıntaş|Ali|A|0000-0002-8932-0160', 'Laker|Rhianna C|RC|', 'Garde|Christian|C|', 'Barrès|Romain|R|0000-0002-0158-519X', 'Zierath|Juleen R|JR|0000-0001-6891-7497']
D000465:Algorithms D000818:Animals D015415:Biomarkers D002460:Cell Line D057906:Circadian Clocks D002940:Circadian Rhythm D019295:Computational Biology D019175:DNA Methylation D004734:Energy Metabolism D057890:Epigenomics D020869:Gene Expression Profiling D059014:High-Throughput Nucleotide Sequencing D051379:Mice D018485:Muscle Fibers, Skeletal D009928:Organ Specificity D011401:Promoter Regions, Genetic D059467:Transcriptome
transcriptomics myotube circadian muscle methylation C2C12 epigenetics