Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni.
Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
['Gencay|Yilmaz Emre|YE|', 'Birk|Tina|T|', 'Sørensen|Martine Camilla Holst|MC|', 'Brøndsted|Lone|L|']
D000362:Agar D000818:Animals D001435:Bacteriophages D002169:Campylobacter Infections D016123:Campylobacter jejuni D002645:Chickens D003470:Culture Media D005243:Feces D011201:Poultry Diseases D010948:Viral Plaque Assay
Isolation Purification Propagation Bacteriophage Campylobacter C. jejuni Titration