Immunological response and protection of mice immunized with plasmid encoding Toxoplasma gondii glycolytic enzyme malate dehydrogenase.
Toxoplasma gondii Malate dehydrogenase (TgMDH) plays an important role as part of the energy production cycle. In this investigation, immunological changes and protection efficiency of this protein delivered as a DNA vaccine have been evaluated. Mice were intramuscularly immunized with pTgMDH, followed by challenge with virulent T. gondii RH strain, 2 weeks after the booster immunization. Compared to the control groups, the results showed that pTgMDH has stimulated specific humoral response as demonstrated by significant high titers of total IgG and subclasses IgG1 and IgG2a , beside IgA and IgM, but not IgE. Analysis of cytokine profiles revealed significant increases of IFN-γ, IL-4 and IL-17, while no significant changes were detected in TGF-β1. In cell-mediated response, both T lymphocytes subpopulations CD4(+) and CD8(+) were positively recruited as significant percentages were recorded in response to immunization with TgMDH. Significant long survival rate, 17 days, has been observed in the TgMDH vaccinated group, in contrast with control groups which died within 8-9 days after challenge. These results demonstrated that TgMDH could induce significant immunological responses leading to a considerable level of protection against acute toxoplasmosis infection.
['Hassan|I A|IA|', 'Wang|S|S|', 'Xu|L|L|', 'Yan|R|R|', 'Song|X|X|', 'XiangRui|L|L|']
D000818:Animals D000913:Antibodies, Protozoan D016207:Cytokines D005260:Female D007273:Injections, Intramuscular D008291:Malate Dehydrogenase D051379:Mice D010957:Plasmids D016052:Protozoan Vaccines D013047:Specific Pathogen-Free Organisms D013601:T-Lymphocytes D014122:Toxoplasma D014124:Toxoplasmosis, Animal D019444:Vaccines, DNA
Toxoplasma gondii malate dehydrogenase DNA vaccines pTgMDH glycolysis