Biomimetic Model of Tumor Microenvironment on Microfluidic Platform.
The "Tumor microenvironment" (TME) is a complex, interacting system of the tumor and its surrounding environment. The TME has drawn more attention recently in attempts to overcome current drug resistance and the recurrence of cancer by understanding the cancer and its microenvironment systematically, beyond past reductionist approaches. However, a lack of experimental tools to dissect the intricate interactions has hampered in-depth research into the TME. Here, a biomimetic TME model using a microfluidic platform is presented, which enables the interaction between TME constituents to be studied in a comprehensive manner. Paracrine interactions of cocultured tumor cell lines (SK-OV-3, MKN-74, and SW620) with primary fibroblasts show marked morphological changes in the tumor cells, depending on the type of tumor cells, and, importantly, the composition of the extracellular matrix. Furthermore, this model allows direct observation of angiogenesis induced by the tumor-stroma interaction. Finally, reconstituting simultaneous angiogenesis and lymphangiogenesis induced by the tumor-stromal interaction with TME mimicking extrinsic factors is enabled. It is believed that the in vitro biomimetic model and the experimental concepts described will help to shed light on the complex biology of the TME.
['Chung|Minhwan|M|', 'Ahn|Jungho|J|', 'Son|Kyungmin|K|', 'Kim|Sudong|S|', 'Jeon|Noo Li|NL|']
D061249:Batch Cell Culture Techniques D040761:Biomimetic Materials D045744:Cell Line, Tumor D004867:Equipment Design D019544:Equipment Failure Analysis D006801:Humans D056656:Lab-On-A-Chip Devices D008422:Materials Testing D009374:Neoplasms, Experimental D023822:Tissue Engineering D059016:Tumor Microenvironment
tumor microenvironments microfluidics angiogenesis lymphangiogenesis 3D coculture