Differential influence of fluoride concentration on the synthesis of bone matrix glycoproteins within mineralizing bone cells in vitro.
OBJECTIVE
This study investigated the influence of fluoride levels on the temporal synthesis of bone-associated glycoproteins, which have been assigned prominent roles in regulating crystal growth, size and shape during the mineralization process.


MATERIALS AND METHODS
Bone marrow stromal cells were isolated from male Wistar rats and cultured under mineralizing conditions, supplemented with 0 M, 10(-7) M or 10(-5) M sodium fluoride. The presence of bone-associated glycoproteins was examined 2-13 days post-reseeding by immunocytochemical localization. Results: All bone-associated glycoproteins increased in 10(-7) M fluoride, compared to untreated controls, particularly at days 6 and 13 in culture. Conversely, higher 10(-5) M fluoride concentrations decreased glycoprotein levels, compared to controls.


CONCLUSIONS
Results highlight a differential effect of fluoride concentration on glycoprotein synthesis by osteoblasts.
['Antonarakis|Gregory S|GS|', 'Moseley|Ryan|R|', 'Waddington|Rachel J|RJ|']
D000818:Animals D001857:Bone Matrix D002113:Calcification, Physiologic D002327:Cariostatic Agents D018929:Cell Culture Techniques D002478:Cells, Cultured D006023:Glycoproteins D007150:Immunohistochemistry D058574:Integrin-Binding Sialoprotein D008297:Male D059630:Mesenchymal Stem Cells D010006:Osteoblasts D015675:Osteocalcin D010012:Osteogenesis D015676:Osteonectin D053495:Osteopontin D051381:Rats D017208:Rats, Wistar D012969:Sodium Fluoride
fluoride bone mineralization bone-associated glycoproteins osteoblasts