The Populus trichocarpa PtHSP17.8 involved in heat and salt stress tolerances.
PtHSP17.8 was regulated by various abiotic stresses. Overexpression of PtHSP17.8 enhanced the tolerance to heat and salt stresses through maintain ROS homeostasis and cooperate with stress-related genes in Arabidopsis. Small heat shock proteins (sHSPs) play important roles in response to diverse biotic and abiotic stresses, especially in heat tolerance. However, limited information is available on the stress tolerance roles of sHSPs in woody species. To explore the function of sHSPs in poplar, we isolated and characterized PtHSP17.8 from Populus trichocarpa. Phylogenetic analysis and subcellular localization revealed that PtHSP17.8 was a cytosolic class I sHSP. The gene expression profile of PtHSP17.8 in various tissues showed that it was significantly accumulated in stem and root, which was consistent with the GUS expression pattern driven by promoter of PtHSP17.8. The expression of PtHSP17.8 could be induced by various abiotic stresses and significantly activated by heat stress. Overexpression of PtHSP17.8 enhanced the tolerance to heat and salt stresses in Arabidopsis. The seedling survival rate, root length, relative water content, antioxidative enzyme activities, proline, and soluble sugar content were increased in transgenic Arabidopsis under heat and salt stresses, but not in normal condition. The co-expression network of PtHSP17.8 were constructed and demonstrated many stress responsive genes included. The stress-related genes in the co-expression network were up-regulated in the PtHSP17.8 overexpression seedlings. These results suggest that PtHSP17.8 confers heat and salt tolerances in plants.
['Li|Jianbo|J|', 'Zhang|Jin|J|', 'Jia|Huixia|H|', 'Li|Yu|Y|', 'Xu|Xiangdong|X|', 'Wang|Lijuan|L|', 'Lu|Mengzhu|M|']
D000595:Amino Acid Sequence D017360:Arabidopsis D018506:Gene Expression Regulation, Plant D053263:Gene Regulatory Networks D017343:Genes, Plant D006358:Hot Temperature D010802:Phylogeny D010940:Plant Proteins D030821:Plants, Genetically Modified D032107:Populus D055049:Salt Tolerance D016415:Sequence Alignment D013312:Stress, Physiological D013347:Subcellular Fractions D000071436:Thermotolerance
Populus PtHSP17.8 Heat Salt Tolerance Arabidopsis