Multilayered Nano-Entrapment of Lipase through Organic-Inorganic Hybrid Formation and the Application in Cost-Effective Biodiesel Production.
Significant components of cost-effective medium for Magnusiomyces capitatus A4C extracellular lipase (ECL) production were optimized via a five-level factorial design. A simplistic, economical, and green approach was adopted for biomimetic mineralization to prepare multilayered nano-entrapped ECL, which were then applied as biocatalysts for the production of fatty acid methyl ester (FAME). The optimal ECL (0.8 mg protein/mL) and CuSO4∙5H2O (1.2 mM) showed the highest capacity for enzyme loading. The ECL-CuSO4-hybrid showed an 89.7% conversion of triacylglycerides into FAME via transesterification and a 98.7% conversion of oleic acid into FAME via esterification at 72 h. The ECL-CuSO4-hybrid gave 65% and 78.7% FAME production after 5 successive reuses via transesterification and esterification reactions, respectively. Therefore, these ECL-inorganic hybrid biocatalysts have high economical potential to be used for the production of biodiesel as the future petrodiesel replacement.
['Baloch|Khurshid Ahmed|KA|', 'Upaichit|Apichat|A|http://orcid.org/0000-0002-3044-3246', 'Cheirsilp|Benjamas|B|']
D056804:Biofuels D004800:Enzymes, Immobilized D005656:Fungal Proteins D008049:Lipase D004718:Saccharomycetales
Nano-entrapment Organic-inorganic hybrid Biodiesel Extracellular lipase Transesterification Esterification