Sini powder () decoction alleviates mood disorder of insomnia by regulating cation-chloride cotransporters in hippocampus.
OBJECTIVE
To investigate the mechanism of Sini Powder () decoction (SND) in the treatment of insomnia.


METHODS
The rats were randomly divided into four groups: control, model, SND-treated, and Estazolamtreated groups (n=15 in each group). Sleep deprivation (SD) rat model was established using the modifified multiple platform method for 14 h per day for 14 days, and the behavior of the rats were observed. Na-K-Cl-cotransporter (NKCC1) and K+/Cl- cotransporter (KCC2) in the hippocampus were tested by immunohistochemistry, real-time polymerase chain reaction, and western blot.


RESULTS
SD rats displayed anxiety-like behavior, which was alleviated by SND. The protein expressions of NKCC1 and KCC2 in the hippocampus were signifificantly decreased in SD rats compared with those in control rats (P<0.05); these proteins were signifificantly increased by SND (P<0.05). The mRNA expression of KCC2 was signifificantly decreased in SD rats (0.62±0.35 vs. 2.29±0.56; P=0.044), while SND showed a tendency to increase the mRNA of KCC2 in SD rats (P>0.05). By contrast, the mRNA expression of NKCC1 was signifificantly increased in the hippocampus of SD rats (6.58±1.54 vs. 2.82±0.32; P=0.011), while SND decreased the mRNA expression of NKCC1 (6.58±1.54 vs. 2.79±0.81; P=0.016).


CONCLUSIONS
Chinese medicine SND could alleviate mood disorder of SD rats by regulating cation-chloride cotransporters, such as NKCC1 and KCC2. These fifindings would have major implications in the mechanism of SND to relieve insomnia.
['Liu|Jing|J|', 'Li|Feng|F|', 'Tang|Xu-Dong|XD|', 'Ma|Jie|J|', 'Mao|Meng|M|', 'Wu|Feng-Zhi|FZ|', 'Bai|Shi-Jing|SJ|', 'Liu|Yan-Yang|YY|', 'Han|Chen-Xia|CX|', 'Li|Xin-Xue|XX|', 'Liu|Yan|Y|', 'Song|Yue-Han|YH|', 'Wu|Zhuo-Yun|ZY|', 'Wang|Feng-Yun|FY|', 'Kang|Nan|N|']
Sini Powder mood disorder insomnia Na-K-Cl-cotransporter K+/Cl- cotransporter