Comparative Mucous miRomics in Cynoglossus semilaevis Related to Vibrio harveyi Caused Infection.
Epidermal mucus is an important barrier and regulating mediator in fish. MicroRNAs (miRNAs) are proved to be involved in various biological processes, also as promising biomarkers for disease diagnosis. Vibrio harveyi has long been a noticeable bacterial pathogen in Cynoglossus semilaevis aquaculture. To find the evidence whether there are indicating miRNAs in mucus and whether the miRNAs are related to infections caused by V. harveyi, miRNA profiles of mucus from V. harveyi infected fish and healthy controls were screened by small RNA sequencing and verified by quantitative real-time PCR. This is the first report about miRNA profiling of flatfish mucus, aiming at illustrating the pathogenesis of V. harveyi caused infection and developing disease-related biomarkers. The results revealed significant differences in expression levels of some miRNAs between infected fish and healthy ones. Three hundred differentially expressed miRNAs were obtained after filtering through FC > 2 or FC < 0.5 and most of the differential miRNAs were downregulated. After verification through qRT-PCR, four unique miRNAs, dre-miR-451, dre-miR-184, dre-miR-205-5p > ssa-miR-205b-5p, and dre-miR-181a-5p > ssa-miR-181a-5p, were identified as V. harveyi infection-related signatures, consistent with sequencing trend. The expression levels of these four miRNAs in the infected fish were all significantly lower than controls. These miRNAs in mucus could be used to differentiate diseased and healthy fish in a non-invasive way with practical value for large-scale disease screening. They also provided new insights into the mechanism underlying the bacterial infections in fish.
['Zhao|Na|N|', 'Jia|Lei|L|', 'Li|Guangli|G|', 'He|Xiaoxu|X|', 'Zhu|Chunhua|C|', 'Zhang|Bo|B|http://orcid.org/0000-0002-9157-8988']
D000818:Animals D015415:Biomarkers D005393:Fish Diseases D005432:Flounder D035683:MicroRNAs D009093:Mucus D014733:Vibrio D014735:Vibrio Infections
Cynoglossus semilaevis Vibrio harveyi Infection Mucus microRNAs