ζ potential changing nanoparticles as cystic fibrosis transmembrane conductance regulator gene delivery system: an in vitro evaluation.
AIM
Aim of the study was the development of ζ potential changing nanoparticles as gene delivery system for the cystic fibrosis transmembrane conductance regulator gene.


METHODS
Chitosan and carboxymethyl cellulose were modified with phosphotyrosine, a substrate for the brush border enzyme alkaline phosphatase. With these synthesized derivatives, different nanoparticle formulations, including the cystic fibrosis transmembrane conductance regulator gene were prepared by ionic gelation.


RESULTS
A change from negative to positive ζ potential after enzymatic cleavage could be observed. Transfection studies with HEK-293 and Caco-2 cells showed transfection rates comparable to Lipofectamine 2000. Transfection efficiencies were significantly decreased when phosphate cleavage and thus ζ potential change was inhibited by phosphatase inhibitor.


CONCLUSION
The developed nanoparticles represent a promising gene delivery system.
['Prüfert|Felix|F|', 'Bonengel|Sonja|S|', 'Köllner|Saskia|S|', 'Griesser|Janine|J|', 'Wilcox|Matthew D|MD|', 'Chater|Peter I|PI|', 'Pearson|Jeffrey P|JP|', 'Bernkop-Schnürch|Andreas|A|']
D018938:Caco-2 Cells D002266:Carboxymethylcellulose Sodium D002470:Cell Survival D002626:Chemistry, Pharmaceutical D048271:Chitosan D019005:Cystic Fibrosis Transmembrane Conductance Regulator D004247:DNA D004926:Escherichia coli D015870:Gene Expression D018014:Gene Transfer Techniques D057809:HEK293 Cells D006801:Humans D008055:Lipids D053758:Nanoparticles D010316:Particle Size D010744:Phosphoric Monoester Hydrolases D010957:Plasmids D013499:Surface Properties D014162:Transfection
ζ potential changing nanoparticles cystic fibrosis transmembrane conductance regulator gene delivery phosphotyrosine phosphatase