Oxygen and differentiation status modulate the effect of X-ray irradiation on physiology and mitochondrial proteome of human neuroblastoma cells.
Cytotoxic effects, including oxidative stress, of low linear energy transfer (LET)-ionizing radiation are often underestimated and studies of their mechanisms using cell culture models are widely conducted with cells cultivated at atmospheric oxygen that does not match its physiological levels in body tissues. Also, cell differentiation status plays a role in the outcome of experiments. We compared effects of 2 Gy X-ray irradiation on the physiology and mitochondrial proteome of nondifferentiated and human neuroblastoma (SH-SY5Y) cells treated with retinoic acid cultivated at 21% and 5% O2. Irradiation did not affect the amount of subunits of OxPhos complexes and other non-OxPhos mitochondrial proteins, except for heat shock protein 70, which was increased depending on oxygen level and differentiation status. These two factors were proven to modulate mitochondrial membrane potential and the bioenergetic status of cells. We suggest, moreover, that oxygen plays a role in the differentiation of human SH-SY5Y cells.
['Džinić|Tamara|T|', 'Hartwig|Sonja|S|', 'Lehr|Stefan|S|', 'Dencher|Norbert A|NA|']
D002454:Cell Differentiation D002470:Cell Survival D015180:Electrophoresis, Gel, Two-Dimensional D006801:Humans D015151:Immunoblotting D053078:Membrane Potential, Mitochondrial D008928:Mitochondria D024101:Mitochondrial Proteins D009447:Neuroblastoma D010100:Oxygen D020543:Proteome D014965:X-Rays
differentiation human neuroblastoma cells retinoic acid Ionizing radiation mitochondria