New insights into the biological activities of Chrysanthemum morifolium: Natural flavonoids alleviate diabetes by targeting α-glucosidase and the PTP-1B signaling pathway.
As dual regulators, the PTP-1B signaling pathway and α-glucosidase slow glucose release and increase the degree of insulin sensitivity, representing a promising therapeutic target for type 2 diabetes. In this study, we systematically examined the in vivo and in vitro anti-diabetic activities of natural flavonoids 1-6 from Chrysanthemum morifolium. Flavonoids 1-6 increased glucose consumption-promoting activity and the phosphorylation of GSK-3β and Akt, and decreased PTP-1B protein level along with slightly inhibitory activity of the PTP-1B enzyme. Moreover, flavonoids 1-2 treatment induced insulin secretion in INS-1 cells. Besides, in vivo study revealed that flavonoids 2 and 5 demonstrated potent anti-hyperglycemic and anti-hyperlipidemic activity, and improved maltose and glucose tolerance. Although flavonoid 2 exhibited lower inhibitory activity against α-glucosidase in vitro, it could deglycosylated in vivo to diosmetin to function as an α-glucosidase inhibitor. Taken together, these results led to the identification of the natural flavonoids 1-6 from C. morifolium as dual regulators of α-glucosidase and the PTP-1B signaling pathway, suggesting their potential application as new oral anti-diabetic drugs or functional food ingredients.
['Chen|Mingzhu|M|', 'Wang|Kaili|K|', 'Zhang|Yinan|Y|', 'Zhang|Mengdi|M|', 'Ma|Yujiao|Y|', 'Sun|Haifeng|H|', 'Jin|Zongxin|Z|', 'Zheng|Hang|H|', 'Jiang|He|H|', 'Yu|Peng|P|', 'Zhang|Yongmin|Y|', 'Sun|Hua|H|']
D000818:Animals D045744:Cell Line, Tumor D002784:Cholesterol D031202:Chrysanthemum D003412:Cricetulus D003921:Diabetes Mellitus, Experimental D005419:Flavonoids D005947:Glucose D065089:Glycoside Hydrolase Inhibitors D007004:Hypoglycemic Agents D008297:Male D051379:Mice D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 D051381:Rats D015398:Signal Transduction D013311:Streptozocin D014280:Triglycerides
Chrysanthemum morifolium Flavonoid Diabetes α-Glucosidase PTP-1B signaling