Long-Term Intravital Imaging of the Cornea, Skin, and Hair Follicle by Multiphoton Microscope.
Multiphoton microscopy allows long-term direct visualization of cells in live animals due to its low photodamage. When coupled with fluorescence protein targeting and second harmonic generation signals from natural collagen as contrast, multiphoton microscopy enables intravital tracing of cells while providing structural information from the extracellular matrix. Compared with conventional histological analysis, it can bring new insight into the cell dynamics in stem cell research. Here, we demonstrate cell imaging and tracing at a single cell resolution in the cornea, skin, and hair follicles using multiphoton microscopy in transgenic mice of which specific cell populations are tagged with fluorescent proteins.
['Wu|Yueh-Feng|YF|', 'Tan|Hsin-Yuan|HY|', 'Lin|Sung-Jan|SJ|']
D000818:Animals D003315:Cornea D004817:Epidermis D018859:Hair Follicle D021621:Imaging, Three-Dimensional D000069416:Intravital Microscopy D008822:Mice, Transgenic D036641:Microscopy, Fluorescence, Multiphoton D012867:Skin
Intravital imaging Cornea Skin Hair follicle Multiphoton microscope (MPM)