Knockdown of Oct4 and Nanog expression inhibits the stemness of pancreatic cancer cells.
Pancreatic cancer is notorious for its difficult diagnosis at early stage and poor recurrence-free prognosis. This study aimed to investigate the possible involvement of Oct4 and Nanog in pancreatic cancer. The high expressions of Oct4 and Nanog in human pancreatic cancer tissues were found to indicate a worse prognostic value of patients. The pancreatic cancer stem cells (PCSCs) that isolated from PANC-1 cell line by flow cytometry exhibited high expressions of Oct4 and Nanog. To investigate whether Oct4 and Nanog play crucial role in maintaining the stemness of PCSCs, double knockdown of Oct4 and Nanog demonstrated that Oct4 and Nanog significantly reduced proliferation, migration, invasion, chemoresistance, and tumorigenesis of PCSCs in vitro and in vivo. The altered expression of the genes related to pancreatic carcinogenesis, metastasis, drug resistance and epithelial-mesenchymal transdifferentiation (EMT) might affect the biological characteristics of PCSCs. Our results suggest that Oct4 and Nanog may serve as a potential marker of prognosis and a novel target of therapy for pancreatic cancer.
['Lu|Yuhua|Y|', 'Zhu|Hui|H|', 'Shan|Haiyan|H|', 'Lu|Junjie|J|', 'Chang|Xu|X|', 'Li|Xiaohong|X|', 'Lu|Jingjing|J|', 'Fan|Xiangjun|X|', 'Zhu|Shajun|S|', 'Wang|Yao|Y|', 'Guo|Qingsong|Q|', 'Wang|Lei|L|', 'Huang|Yan|Y|', 'Zhu|Mingyan|M|', 'Wang|Zhiwei|Z|']
D000818:Animals D000964:Antimetabolites, Antineoplastic D017209:Apoptosis D063646:Carcinogenesis D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D003841:Deoxycytidine D019008:Drug Resistance, Neoplasm D005260:Female D055785:Gene Knockdown Techniques D057809:HEK293 Cells D018398:Homeodomain Proteins D006801:Humans D053208:Kaplan-Meier Estimate D051379:Mice D008807:Mice, Inbred BALB C D008819:Mice, Nude D000071317:Nanog Homeobox Protein D009361:Neoplasm Invasiveness D009368:Neoplasm Transplantation D014411:Neoplastic Stem Cells D050814:Octamer Transcription Factor-3 D010190:Pancreatic Neoplasms D034622:RNA Interference D047368:Tumor Burden
Oct4 Nanog Pancreatic cancer Cancer stem cells