Preliminary Study on Gene Expression of Chitinase-Like Cytokines in Human Airway Epithelial Cell Under Chitin and Chitosan Microparticles Treatment.
Small-sized chitin and chitosan microparticles (MPs) reduce allergic inflammation. We examined the capacity of these glycans to stimulate A549 human airway epithelial cells to determine the feasibility of using of these glycans as allergic therapeutic modality. A549 cells were treated with MPs and then expressions levels of chitinase domain-containing 1 (CHID1) and chitinase 3-like 1 (CHI3L1) genes were determined by quantitative real-time PCR. IL-6 production was measured by ELISA. Chitin MPs resulted in upregulation of CHI3L1 expression by 35.7-fold while mRNA expression did not change with chitosan MPs. Compared to the untreated group, production of IL-6 was significantly decreased in the chitosan MPs-treated group, but chitin MPs treatment cause elevation of IL-6 level. This study demonstrates that chitin potently induces CHI3L1 expression, but chitosan is relatively inert. This effect and inhibition of pro-inflammatory cytokine (IL-6) suggest that chitosan MPs may possess more potential for therapeutic uses in human airway allergic inflammation.
['Alimohammadi|Masumeh|M|', 'Yeganeh|Farshid|F|', 'Haji Molla Hoseini|Mostafa|M|']
D000072283:A549 Cells D001249:Asthma D002686:Chitin D000071451:Chitinase-3-Like Protein 1 D002688:Chitinases D048271:Chitosan D016207:Cytokines D016503:Drug Delivery Systems D004847:Epithelial Cells D015870:Gene Expression D006801:Humans D007249:Inflammation D015850:Interleukin-6 D012137:Respiratory System
chitin chitosan allergic inflammation chitinase-like proteins