Chromosome Preparation for Myeloid Malignancies.
Many cases of myeloid malignancies are associated with recurring cytogenetic aberrations. Chromosomal analysis can aid in diagnosis, predict prognosis, and disclose subsequent clonal evolution. Three different cell culture methods: direct harvest, nonsynchronized culture, and synchronized culture are usually prepared if the nucleated cell counts in marrow blood are sufficient. Synchronized culture is the first choice of method in myeloid malignancies, whereas the direct method can be omitted if the cell count is low. The aseptic culture technique is strictly followed until harvesting procedure. For synchronized culture, uridine and fluorodeoxyuridine are added as blocking reagents and released by thymidine on the following day. Harvesting steps of the cultures involved colcemid exposure, hypotonic treatment, and Carnoy's fixation. The cells are then ready for slide making and banding for chromosomal analysis.
['Hui|Eleanor K C|EK|', 'Wan|Thomas S K|TS|', 'Ng|Margaret H L|MH|']
D001853:Bone Marrow D001854:Bone Marrow Cells D002452:Cell Count D002478:Cells, Cultured D002869:Chromosome Aberrations D020732:Cytogenetic Analysis D006801:Humans D009196:Myeloproliferative Disorders
Chromosome preparation Myeloid malignancies Synchronization culture Cytogenetic culture Metaphase harvesting