Reverse-transcription recombinase-aided amplification assay for H7 subtype avian influenza virus.
H7 subtype avian influenza virus infection is an emerging zoonosis in some Asian countries and an important avian disease worldwide. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. In this study, we developed a reverse-transcription recombinase-aided amplification assay for the detection of H7 subtype avian influenza virus. Assays were performed at a single temperature (39°C), and the results were obtained within 20 min. The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus, which are the other main respiratory viruses affecting birds. The analytical sensitivity was 102 RNA copies per reaction at a 95% probability level according to probit regression analysis, with 100% specificity. Compared with published reverse-transcription quantitative real-time polymerase chain reaction assays, the κ value of the reverse-transcription recombinase-aided amplification assay in 342 avian clinical samples was 0.988 (p < .001). The sensitivity for avian clinical sample detection was 100% (95%CI, 90.40%-100%), and the specificity was 99.96% (95%CI, 97.83%-99.98%). These results indicated that our reverse-transcription recombinase-aided amplification assay may be a valuable tool for detecting avian influenza H7 subtype virus.
['Wang|Suchun|S|', 'Huang|Baoxu|B|', 'Ma|Xuejun|X|', 'Liu|Ping|P|', 'Wang|Yang|Y|', 'Zhang|Xiaoguang|X|', 'Zhu|Lin|L|', 'Fan|Qingying|Q|', 'Sun|Yawei|Y|', 'Wang|Kaicheng|K|https://orcid.org/0000-0002-1115-473X']
D000818:Animals D001717:Birds D006801:Humans D009980:Influenza A virus D005585:Influenza in Birds D007251:Influenza, Human D021141:Nucleic Acid Amplification Techniques D060888:Real-Time Polymerase Chain Reaction D045522:Recombinases D020133:Reverse Transcriptase Polymerase Chain Reaction D048348:Reverse Transcription D012680:Sensitivity and Specificity D015047:Zoonoses
reverse-transcription recombinase-aided amplification assay H7 subtype avian influenza