The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells.
Parvovirus B19 (B19V) is a widespread human pathogen possessing a high tropism for erythroid precursor cells. However, the persistence or active replication of B19V in endothelial cells (EC) has been detected in diverse human pathologies. The VP1 unique region (VP1u) of the viral capsid has been reported to act as a major determinant of viral tropism for erythroid precursor cells. Nevertheless, the interaction of VP1u with EC has not been studied. We demonstrate that recombinant VP1u is efficiently internalized by rats' pulmonary trunk blood vessel-derived EC in vitro compared to the human umbilical vein EC line. The exposure to VP1u was not acutely cytotoxic to either human- or rat-derived ECs, but led to the upregulation of cellular stress signaling-related pathways. Our data suggest that high levels of circulating B19V during acute infection can cause endothelial damage, even without active replication or direct internalization into the cells.
['Rinkūnaitė|Ieva|I|0000-0001-9956-4022', 'Šimoliūnas|Egidijus|E|0000-0001-5440-3591', 'Bironaitė|Daiva|D|', 'Rutkienė|Rasa|R|', 'Bukelskienė|Virginija|V|', 'Meškys|Rolandas|R|0000-0003-3125-9827', 'Bogomolovas|Julius|J|0000-0001-8344-1909']
D000818:Animals D002470:Cell Survival D002478:Cells, Cultured D061307:Human Umbilical Vein Endothelial Cells D006801:Humans D020935:MAP Kinase Signaling System D016732:Parvovirus B19, Human D011651:Pulmonary Artery D051381:Rats D017208:Rats, Wistar D011994:Recombinant Proteins D014760:Viral Fusion Proteins
parvovirus B19 endothelial cells VP1u endothelial stress