FGF1 improves functional recovery through inducing PRDX1 to regulate autophagy and anti-ROS after spinal cord injury.
Fibroblast growth factor 1 (FGF1) is thought to exert protective and regenerative effects on neurons following spinal cord injury (SCI), although the mechanism of these effects is not well understood. The use of FGF1 as a therapeutic agent is limited by its lack of physicochemical stability and its limited capacity to cross the blood-spinal cord barrier. Here, we demonstrated that overexpression of FGF1 in spinal cord following SCI significantly reduced tissue loss, protected neurons in the ventricornu, ameliorated pathological morphology of the lesion, dramatically improved tissue recovery via neuroprotection, and promoted axonal regeneration and remyelination both in vivo and in vivo. In addition, the autophagy and the expression levels of PRDX1 (an antioxidant protein) were induced by AAV-FGF1 in PC12 cells after H2 O2 treatment. Furthermore, the autophagy levels were not changed in PRDX1-suppressing cells that were treated by AAV-FGF1. Taken together, these results suggest that FGF1 improves functional recovery mainly through inducing PRDX1 expression to increase autophagy and anti-ROS activity after SCI.
['Li|Jiawei|J|', 'Wang|Qingqing|Q|', 'Cai|Hanxiao|H|', 'He|Zili|Z|', 'Wang|Haoli|H|', 'Chen|Jian|J|', 'Zheng|Zengming|Z|', 'Yin|Jiayu|J|', 'Liao|Zhiyong|Z|', 'Xu|Huazi|H|', 'Xiao|Jian|J|', 'Gong|Fanghua|F|0000-0002-8905-4894']
D000818:Animals D001343:Autophagy D001369:Axons D016764:Cell Polarity D003599:Cytoskeleton D000229:Dependovirus D005260:Female D016220:Fibroblast Growth Factor 1 D005822:Genetic Vectors D009043:Motor Activity D009416:Nerve Regeneration D018696:Neuroprotective Agents D016716:PC12 Cells D054464:Peroxiredoxins D051381:Rats D017207:Rats, Sprague-Dawley D017382:Reactive Oxygen Species D020127:Recovery of Function D000074586:Remyelination D013119:Spinal Cord Injuries
functional recovery autophagy spinal cord injury fibroblast growth factor 1