Preparation of Chito-Oligomers by Hydrolysis of Chitosan in the Presence of Zeolite as Adsorbent.
An increasing interest has recently been shown to use chitin/chitosan oligomers (chito-oligomers) in medicine and food fields because they are not only water-soluble, nontoxic, and biocompatible materials, but they also exhibit numerous biological properties, including antibacterial, antifungal, and antitumor activities, as well as immuno-enhancing effects on animals. Conventional depolymerization methods of chitosan to chito-oligomers are either chemical by acid-hydrolysis under harsh conditions or by enzymatic degradation. In this work, hydrolysis of chitosan to chito-oligomers has been achieved by applying adsorption-separation technique using diluted HCl in the presence of different types of zeolite as adsorbents. The chito-oligomers were retrieved from adsorbents and characterized by differential scanning calorimetry (DSC), liquid chromatography/mass spectroscopy (LC/MS), and ninhydrin test.
['Ibrahim|Khalid A|KA|', 'El-Eswed|Bassam I|BI|', 'Abu-Sbeih|Khaleel A|KA|', 'Arafat|Tawfeeq A|TA|', 'Al Omari|Mahmoud M H|MM|', 'Darras|Fouad H|FH|', 'Badwan|Adnan A|AA|']
D000900:Anti-Bacterial Agents D000935:Antifungal Agents D000970:Antineoplastic Agents D001672:Biocompatible Materials D002152:Calorimetry, Differential Scanning D002686:Chitin D048271:Chitosan D002853:Chromatography, Liquid D006851:Hydrochloric Acid D006868:Hydrolysis D007202:Indicators and Reagents D013058:Mass Spectrometry D009555:Ninhydrin D017641:Zeolites
chito-oligomers hydrolysis chitosan zeolite depolymerization