Gingival crevicular fluid and plasma oxidative stress markers and TGM-2 levels in chronic periodontitis.
OBJECTIVE
This study was aimed to evaluate the gingival crevicular fluid (GCF) and plasma transglutaminase-2 (TGM-2), total antioxidant capacity (TAC), total oxidant status (TOS), ferric reducing antioxidant power (FRAP) and thiobarbituric acid reactive substances (TBARS) in patients with chronic periodontal disease.


MATERIALS AND METHODS
Twenty patients with chronic periodontitis (CP), 20 patients with gingivitis and 20 healthy subjects were enrolled in the study. Clinical periodontal parameters including probing depth, clinical attachment level, plaque index and papillary bleeding index were recorded. GCF and plasma levels of TGM-2, TAC, TOS, TBARS and FRAP were analyzed.


RESULTS
GCF TGM-2 was significantly lower in CP group than in gingivitis patients (P=0.006). GCF FRAP in CP and gingivitis groups was significantly lower than in healthy subjects (P<0.001). Plasma FRAP level was lower in gingivitis group when compared to healthy subjects (P=0.003). There was no significant difference in GCF and plasma TAC, TOS, TBARS and plasma TGM-2 levels among the study groups (P>0.05). GCF TGM-2 level was positively correlated with GCF TAC and negatively correlated with CAL.


CONCLUSIONS
Decreased FRAP in GCF and plasma indicating lower antioxidant status of CP patients might suggest the role of oxidative stress in periodontitis. GCF TGM-2 data might suggest that TGM2 is associated with stabilization of the extracellular matrix and wound healing in periodontium rather than gingival inflammation.
['Becerik|Sema|S|', 'Öztürk|Veli Özgen|VÖ|', 'Celec|Peter|P|', 'Kamodyova|Natalia|N|', 'Atilla|Gül|G|', 'Emingil|Gülnur|G|']
D000328:Adult D000975:Antioxidants D015415:Biomarkers D016022:Case-Control Studies D055113:Chronic Periodontitis D005260:Female D019204:GTP-Binding Proteins D005883:Gingival Crevicular Fluid D005891:Gingivitis D006801:Humans D008297:Male D008875:Middle Aged D016877:Oxidants D010084:Oxidation-Reduction D018384:Oxidative Stress D010512:Periodontal Index D000091345:Protein Glutamine gamma Glutamyltransferase 2 D017392:Thiobarbituric Acid Reactive Substances D011503:Transglutaminases
Gingival crevicular fluid Oxidative stress Chronic periodontitis Transglutaminase-2