A star-shaped porphyrin-arginine functionalized poly(L-lysine) copolymer for photo-enhanced drug and gene co-delivery.
The co-delivery of drug and gene has become the primary strategy in cancer and other disease therapy. To co-deliver hydrophobic drug and functional gene efficiently into tumor cells, a star-shaped copolymer (PP-PLLD-Arg) with a photochemical internalization effect consisting of a porphyrin (PP) core and arginine-functionalized poly(L-lysine) dendron (PLLD-Arg) arms has been designed, and used to co-deliver docetaxel (DOC) and MMP-9 shRNA plasmid for nasopharyngeal cancer therapy. It was found that PP-PLLD-Arg/MMP-9 nanocomplex showed the photo-enhanced gene transfection efficiency in vitro, and could mediate a significant reduce of MMP-9 protein expression in HNE-1 cells. For co-delivery analysis, the obtained PP-PLLD-Arg/DOC/MMP-9 complexes could induce a more significant apoptosis than DOC or MMP-9 used only, and decreased invasive capacity of HNE-1 cells. Moreover, the star-shaped copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k in the hemolysis and MTT assays, and also showed a good biocompatibility in vivo. Therefore, PP-PLLD-Arg with suited irradiation is a promising non-toxic and photo-inducible effective drug and gene delivery strategy, which should be encouraged in tumor therapy.
['Ma|Dong|D|', 'Lin|Qian-Ming|QM|', 'Zhang|Li-Ming|LM|', 'Liang|Yuan-Yuan|YY|', 'Xue|Wei|W|']
D000818:Animals D017209:Apoptosis D001120:Arginine D001672:Biocompatible Materials D045744:Cell Line, Tumor D002850:Chromatography, Gel D000077143:Docetaxel D016503:Drug Delivery Systems D005260:Female D005434:Flow Cytometry D018014:Gene Transfer Techniques D006461:Hemolysis D006801:Humans D008027:Light D009682:Magnetic Resonance Spectroscopy D020780:Matrix Metalloproteinase 9 D051379:Mice D010316:Particle Size D011107:Polylysine D011166:Porphyrins D011485:Protein Binding D012333:RNA, Messenger D055672:Static Electricity D043823:Taxoids D014162:Transfection
Photo-enhanced Co-delivery Porphyrin Arginine Lysine Star polymers