Nonclinical models for antituberculosis drug development: a landscape analysis.
BACKGROUND
Several nonclinical drug-development tools (DDTs) have been used for antituberculosis drug development over several decades. The role of the DDTs used for evaluating the efficacy of antituberculosis drug combinations and the gaps in the evidence base for which new tools or approaches are needed are as yet undefined.


METHODS
We performed a landscape analysis based on a comprehensive literature review to create evidence based guidelines.


RESULTS
There are 3 important questions that a DDT should answer with regard to antituberculosis drugs: What combination(s) of drugs will be most effective? What dose(s) and schedule(s) of each drug should be administered? and What duration(s) of treatment will be efficacious? Four DDTs were identified as having a track record to answer these questions: in vitro susceptibility tests, the hollow fiber system model of tuberculosis, mice, and guinea pigs. No single nonclinical in vitro or animal model recapitulates all aspects of human tuberculosis. Therefore, a combination of models is recommended for drug development. Gaps identified include the need for standardization of nonclinical model experiments, evaluation of animal models with pathology more similar to that in humans, and identification of experimental quantitative output in the DDTs that correlates with sterilizing effect in humans.


CONCLUSIONS
There is a need for formal quantitative analyses of how well DDTs forecast clinical outcomes.
['Gumbo|Tawanda|T|', 'Lenaerts|Anne J|AJ|', 'Hanna|Debra|D|', 'Romero|Klaus|K|', 'Nuermberger|Eric|E|']
D000818:Animals D000995:Antitubercular Agents D004195:Disease Models, Animal D004338:Drug Combinations D006801:Humans D014376:Tuberculosis
antituberculosis drug development hollow fiber system model of tuberculosis drug regimen design guinea pig tuberculosis model mouse tuberculosis model