G-Protein-Coupled Receptor Gpr17 Expression in Two Multiple Sclerosis Remyelination Models.
In multiple sclerosis patients, demyelination is prominent in both the white and gray matter. Chronic clinical deficits are known to result from acute or chronic injury to the myelin sheath and inadequate remyelination. The underlying molecular mechanisms of remyelination and its failure remain currently unclear. Recent studies have recognized G protein-coupled receptor 17 (GPR17) as an important regulator of oligodendrocyte development and remyelination. So far, the relevance of GPR17 for myelin repair was mainly tested in remyelinating white matter lesions. The relevance of GPR17 for gray matter remyelination as well as remyelination of chronic white matter lesions was not addressed so far. Here, we provide a detailed characterization of GPR17 expression during experimental de- and remyelination. Experimental lesions with robust and limited endogenous remyelination capacity were established by either acute or chronic cuprizone-induced demyelination. Furthermore, remyelinating lesions were induced by the focal injection of lysophosphatidylcholine (LPC) into the corpus callosum. GPR17 expression was analyzed by complementary techniques including immunohistochemistry, in situ hybridization, and real-time PCR. In control animals, GPR17+ cells were evenly distributed in the corpus callosum and cortex and displayed a highly ramified morphology. Virtually all GPR17+ cells also expressed the oligodendrocyte-specific transcription factor OLIG2. After acute cuprizone-induced demyelination, robust endogenous remyelination was evident in the white matter corpus callosum but not in the gray matter cortex. Endogenous callosal remyelination was paralleled by a robust induction of GPR17 expression which was absent in the gray matter cortex. Higher numbers of GPR17+ cells were as well observed after LPC-induced focal white matter demyelination. In contrast, densities of GPR17+ cells were comparable to control animals after chronic cuprizone-induced demyelination indicating quiescence of this cell population. Our findings demonstrate that GPR17 expression induction correlates with acute demyelination and sufficient endogenous remyelination. This strengthens the view that manipulation of this receptor might be a therapeutic opportunity to support endogenous remyelination.
['Nyamoya|Stella|S|', 'Leopold|Patrizia|P|', 'Becker|Birte|B|', 'Beyer|Cordian|C|', 'Hustadt|Fabian|F|', 'Schmitz|Christoph|C|', 'Michel|Anne|A|', 'Kipp|Markus|M|']
D000818:Animals D002540:Cerebral Cortex D003337:Corpus Callosum D003471:Cuprizone D003711:Demyelinating Diseases D004195:Disease Models, Animal D004681:Encephalomyelitis, Autoimmune, Experimental D005260:Female D066128:Gray Matter D008297:Male D051379:Mice D009103:Multiple Sclerosis D009186:Myelin Sheath D009419:Nerve Tissue Proteins D043562:Receptors, G-Protein-Coupled D000074586:Remyelination D066127:White Matter
Gpr17 Multiple sclerosis Remyelination LPC CNS Cuprizone