Comparison of extraction methods for the identification and quantification of polyphenols in virgin olive oil by ultra-HPLC-QToF mass spectrometry.
In this work, liquid-liquid extraction (LLE) and solid phase extraction (SPE), of polyphenols from a VOO sample were optimised by a Plackett-Burman experimental design; then the two extraction techniques capabilities were compared. By using HPLC-DAD, the extraction ability of SPE with the diol phase and LLE were similar. The two methods were further evaluated with ultra HPLC-ESI QToF in negative ion mode by recoveries of standards and matched comparison of the peak area of 40 identified and 27 unidentified compounds. Conclusions indicate that LLE gives better recoveries for highly polar, non-polar, and some polyphenols suspected to contain a nitrogen atom, while for the others the two methods seem to be equally suitable. The presence of nitrogen-containing polyphenols was confirmed in positive ionisation mode in LLE extract, whereas in the SPE extract they were not present. One of them was tentatively identified as a compound containing tyrosine and methyl-decarboxymetyl-eleanoic acid moieties.
['Capriotti|Anna Laura|AL|', 'Cavaliere|Chiara|C|', 'Crescenzi|Carlo|C|', 'Foglia|Patrizia|P|', 'Nescatelli|Riccardo|R|', 'Samperi|Roberto|R|', 'Laganà|Aldo|A|']
D002851:Chromatography, High Pressure Liquid D059625:Liquid-Liquid Extraction D013058:Mass Spectrometry D000069463:Olive Oil D010938:Plant Oils D059808:Polyphenols D052616:Solid Phase Extraction
Polyphenols Virgin olive oil Solid phase extraction (SPE) Experimental design LC–MS Liquid–liquid extraction (LLE)