Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum.
Genome modifications in microalgae are becoming a widespread and mandatory tool for research in both fundamental and applied biology. Among genome editing methods in these photosynthetic organisms, CRISPR/Cas9 offers a specific, powerful and efficient tool for genome engineering by inducing mutations in targeted regions of the genome. Here we described a protocol that allows the generation of knockout mutants by CRISPR/Cas9 in the diatom Phaeodactylum tricornutum using biolistic transformation.
['Allorent|Guillaume|G|', 'Guglielmino|Erika|E|', 'Giustini|Cécile|C|', 'Courtois|Florence|F|']
D019470:Biolistics D064113:CRISPR-Cas Systems D002467:Cell Nucleus D060365:Chloroplast Proteins D017377:Diatoms D000072669:Gene Editing D055786:Gene Knockout Techniques D009154:Mutation
CRISPR/Cas9 Phaeodactylum tricornutum Genome editing Biolistic transformation