Integrative Transcriptomic and Small RNA Sequencing Reveals Immune-Related miRNA-mRNA Regulation Network for Soybean Meal-Induced Enteritis in Hybrid Grouper, Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂.
A 10-week feeding experiment was conducted to reveal the immune mechanism for soybean meal-induced enteritis (SBMIE) in hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂. Four isonitrogenous and isolipidic diets were formulated by replacing 0, 10, 30, and 50% fish meal protein with soybean meal (namely FM, SBM10, SBM30, and SBM50, respectively). The weight gain rate of the SBM50 group was significantly lower than those of the other groups. Plica height, muscular layer thickness, and goblet cells of the distal intestine in the SBM50 group were much lower than those in the FM group. The intestinal transcriptomic data, including the transcriptome and miRNAome, showed that a total of 6,390 differentially expressed genes (DEGs) and 92 DEmiRNAs were identified in the SBM50 and FM groups. DEmiRNAs (10 known and 1 novel miRNAs) and their DE target genes were involved in immune-related phagosome, natural killer cell-mediated cytotoxicity, Fc gamma R-mediated phagocytosis, and the intestinal immune network for IgA production pathways. Our study is the first to offer transcriptomic and small RNA profiling for SBMIE in hybrid grouper. Our findings offer important insights for the understanding of the RNA profile and further elucidation of the underlying molecular immune mechanism for SBMIE in carnivorous fish.
['He|Yuanfa|Y|', 'Ye|Guanlin|G|', 'Chi|Shuyan|S|', 'Tan|Beiping|B|', 'Dong|Xiaohui|X|', 'Yang|Qihui|Q|', 'Liu|Hongyu|H|', 'Zhang|Shuang|S|']
D000818:Animals D003602:Cytotoxicity, Immunologic D004751:Enteritis D005393:Fish Diseases D005399:Fishes D020869:Gene Expression Profiling D005786:Gene Expression Regulation D053263:Gene Regulatory Networks D007109:Immunity D007070:Immunoglobulin A D055048:Integrative Medicine D035683:MicroRNAs D010587:Phagocytosis D012333:RNA, Messenger D017423:Sequence Analysis, RNA
hybrid grouper RNA-seq immune response inflammation intestinal health