Biodistribution and Tumor Uptake of 67Ga-Nimotuzumab in a Malignant Pleural Mesothelioma Xenograft.
Malignant pleural mesothelioma (MPM) is the most common tumor of the pulmonary pleura. It is a rare and aggressive malignancy, generally associated with continuous occupational exposure to asbestos. Only a multimodal-approach to treatment, based on surgical resection, chemotherapy and/or radiation, has shown some benefits. However, the survival rate remains low. Nimotuzumab (h-R3), an anti-EGFR (epidermal growth factor receptor) humanized antibody, is proposed as a promising agent for the treatment of MPM. The aim of this research was to implement a procedure for nimotuzumab radiolabeling to evaluate its biodistribution and affinity for EGF (epidermal growth factor) receptors present in a mesothelioma xenograft. Nimotuzumab was radiolabeled with 67Ga; radiolabel efficiency, radiochemical purity, serum stability, and biodistribution were evaluated. Biodistribution and tumor uptake imaging studies by microSPECT/CT in mesothelioma xenografts revealed constant nimotuzumab uptake at the tumor site during the first 48 h after drug administration. In vivo studies using MPM xenografts showed a significant uptake of this radioimmunoconjugate, which illustrates its potential as a biomarker that could promote its theranostic use in patients with MPM.
['Izquierdo-Sánchez|Vanessa|V|', 'Muñiz-Hernández|Saé|S|', 'Vázquez-Becerra|Héctor|H|', 'Pacheco-Yepez|Judith|J|', 'Romero-Piña|Mario E|ME|', 'Arrieta|Oscar|O|', 'Medina|Luis Alberto|LA|0000-0001-9301-5823']
D000818:Animals D061067:Antibodies, Monoclonal, Humanized D045744:Cell Line, Tumor D019788:Fluorodeoxyglucose F18 D005710:Gallium Radioisotopes D006801:Humans D021621:Imaging, Three-Dimensional D008099:Liver D008175:Lung Neoplasms D008297:Male D008654:Mesothelioma D000086002:Mesothelioma, Malignant D008819:Mice, Nude D010997:Pleural Neoplasms D000072078:Positron Emission Tomography Computed Tomography D014018:Tissue Distribution D015899:Tomography, Emission-Computed, Single-Photon D023041:Xenograft Model Antitumor Assays
malignant pleural mesothelioma nimotuzumab radioimmunoconjugates biomarkers molecular imaging