Production of inflammatory cytokines in response to diphtheria-pertussis-tetanus (DPT), haemophilus influenzae type b (Hib), and 7-valent pneumococcal (PCV7) vaccines.
Haemophilus influenzae type b (Hib) and 7-valent pneumococcal (PCV7) vaccines both became recommended in Japan in 2010. In this study, cytokine production was investigated in peripheral blood mononuclear cells (PBMCs) cultures stimulated with diphtheria and tetanus toxoids combined with acellular pertussis vaccine (DPT), Hib, and PCV7 separately or concurrent different combinations, all as final off-the-shelf vaccines without the individual vaccine components as controls. Higher IL-1β levels were produced when cultures were stimulated with PCV than with DPT or Hib, and the concurrent stimulation including PCV7 enhanced the production of IL-1β. Although Hib induced higher levels of IL-6, no significant difference was observed in IL-6 production with the concurrent stimulation. The concurrent stimulation with Hib/PCV7 and DPT/Hib/PCV7 produced higher levels of TNF-α and human G-CSF. Cytokine profiles were examined in serum samples obtained from 61 vaccine recipients with febrile reactions and 18 recipients without febrile illness within 24 h of vaccination. No significant difference was observed in cytokine levels of IL-1β, IL-4, IL-6, IL-10, IL-12, IFN-γ, MIP-1, TNF-α, and prostaglandin E2 (PGE2) in sera between the two groups. However, significantly higher levels of human G-CSF were observed in recipients with febrile illness than in those without febrile reactions. Further investigations of the significance of elevated serum G-CSF levels are required in vaccine recipients with febrile illness.
['Kashiwagi|Yasuyo|Y|', 'Miyata|Akiko|A|', 'Kumagai|Takuji|T|', 'Maehara|Kouji|K|', 'Suzuki|Eitarou|E|', 'Nagai|Takao|T|', 'Ozaki|Takao|T|', 'Nishimura|Naoko|N|', 'Okada|Kenji|K|', 'Kawashima|Hisashi|H|', 'Nakayama|Tetsuo|T|']
D002478:Cells, Cultured D002648:Child D002675:Child, Preschool D016207:Cytokines D015721:Diphtheria-Tetanus-Pertussis Vaccine D018073:Haemophilus Vaccines D000069443:Heptavalent Pneumococcal Conjugate Vaccine D006801:Humans D007223:Infant D007564:Japan D007963:Leukocytes, Mononuclear D008297:Male D022242:Pneumococcal Vaccines
cytokine DPT Hib PCV7 peripheral blood mononuclear cells PBMCs Diphtheria and tetanus toxoids combined with acellular pertussis vaccine 7-valent pneumococcal conjugated vaccine Haemophilus influenzaetype b T-conjugated vaccine innate immunity