Caffeine Protects Skin from Oxidative Stress-Induced Senescence through the Activation of Autophagy.
Skin cells are vulnerable to oxidative stress-induced senescence, which may lead to abnormal aging or aging-related disorders. Therefore, strategies that can ameliorate oxidative stress-induced senescence are expected to protect skin from damage, holding the promise of treating skin diseases in the clinic. This study aims to investigate whether caffeine, a well-known purine alkaloid, is able to prevent skin from oxidative stress-induced senescence, and to explore the underlying molecular mechanisms. Methods: A free radical inducer 2,2'-Azobis (2-amidinopropane) dihydrochloride (AAPH) was used to induce oxidative stress and cellular senescence in both transformed skin cells and in normal human epidermal keratinocytes (NHEKs). Ultraviolet (UV) irradiation was established as the in vivo oxidative stress model in mouse skin tissues. Cellular senescence was determined by SA β-galactosidase staining, immunofluorescence and western blotting. Activation of autophagy was confirmed by western blotting, immunofluorescence, and transmission electron microscopy. Reactive oxygen species (ROS) detection by commercial kits, gene knockdown by RNA interference (RNAi) and receptor activation/inactivation by agonist/antagonist treatment were applied in mechanistic experiments. Results: We report that AAPH induced senescence in both transformed skin cells and in NHEKs. Similarly, UV irradiation induced senescence in mouse skin tissues. Remarkably, low dose of caffeine (<10 μM) suppressed cellular senescence and skin damage induced by AAPH or UV. Mechanistically, caffeine facilitated the elimination of ROS by activating autophagy. Using a combination of RNAi and chemical treatment, we demonstrate that caffeine activates autophagy through a series of sequential events, starting from the inhibition of its primary cellular target adenosine A2a receptor (A2AR) to an increase in the protein level of Sirtuin 3 (SIRT3) and to the activation of 5' adenosine monophosphate-activated protein kinase (AMPK). Oral administration of caffeine increased the protein level of SIRT3, induced autophagy, and reduced senescence and tissue damage in UV-irradiated mouse skin. On the other hand, co-administration with autophagy inhibitors attenuated the protective effect of caffeine on UV-induced skin damage in mice. Conclusion: The results reveal that caffeine protects skin from oxidative stress-induced senescence through activating the A2AR/SIRT3/AMPK-mediated autophagy. Our study not only demonstrated the beneficial effect of caffeine using both in vitro and in vivo models, but also systematically investigated the underlying molecular mechanisms. These discoveries implicate the potential of caffeine in the protection of skin disease.
['Li|Yi-Fang|YF|', 'Ouyang|Shu-Hua|SH|', 'Tu|Long-Fang|LF|', 'Wang|Xi|X|', 'Yuan|Wei-Lin|WL|', 'Wang|Guo-En|GE|', 'Wu|Yan-Ping|YP|', 'Duan|Wen-Jun|WJ|', 'Yu|Hong-Min|HM|', 'Fang|Zhong-Ze|ZZ|', 'Kurihara|Hiroshi|H|', 'Zhang|Youwei|Y|', 'He|Rong-Rong|RR|']
D000375:Aging D000578:Amidines D000818:Animals D000975:Antioxidants D001343:Autophagy D002110:Caffeine D002478:Cells, Cultured D004195:Disease Models, Animal D006801:Humans D051379:Mice D008962:Models, Theoretical D016877:Oxidants D018384:Oxidative Stress D012867:Skin D012871:Skin Diseases D016896:Treatment Outcome D014466:Ultraviolet Rays
caffeine autophagy cellular senescence UV radiation skin aging