Performance of nanocellulose-producing bacterial strains in static and agitated cultures with different starting pH.
The impact of strain selection and culture conditions on bacterial nanocellulose (BNC) productivity and quality was investigated by using four strains, static and agitated cultures, and an initial pH in the range 4-6. With agitation, strain DHU-ATCC-1 displayed highest productivity [1.14 g/(L × d)]. In static cultures, DHU-ZGD-1186 exhibited superior BNC yield on consumed glucose (0.79 g/g), and lowest by-product formation with respect to gluconic acids [≤0.07 g/(L × d)]. By-product formation typically decreased in the order gluconic acid >2-keto-gluconic acid >5-keto-gluconic acid, and was lowest in cultures with high initial pH. The BNC from DHU-ZGD-1186 exhibited higher average viscometric degree of polymerization (DPv), higher crystallinity index, and higher tear index. In conclusion, both strain selection and cultivation conditions had an impact on BNC productivity and properties. Productivity, DPv, crystallinity, and mechanical strength of BNC from agitated cultures could be similar to or even higher than the corresponding values for static cultures.
['Chen|Genqiang|G|', 'Wu|Guochao|G|', 'Chen|Lin|L|', 'Wang|Wei|W|', 'Hong|Feng F|FF|', 'Jönsson|Leif J|LJ|']
D018929:Cell Culture Techniques D002482:Cellulose D005285:Fermentation D041822:Gluconacetobacter xylinus D005942:Gluconates D005947:Glucose D006863:Hydrogen-Ion Concentration
Bacterial nanocellulose Gluconic acids Cultivation conditions Komagataeibacter xylinus Nanocellulose quality Strain comparison