Analysis of Axonemal Assembly During Ciliary Regeneration in Chlamydomonas.
Chlamydomonas reinhardtii is an outstanding model genetic organism for study of assembly of cilia. Here, methods are described for synchronization of ciliary regeneration in Chlamydomonas to analyze the sequence in which ciliary proteins assemble. In addition, the methods described allow analysis of the mechanisms involved in regulation of ciliary length, the proteins required for ciliary assembly, and the temporal expression of genes encoding ciliary proteins. Ultimately, these methods can contribute to discovery of conserved genes that when defective lead to abnormal ciliary assembly and human disease.
['Hunter|Emily L|EL|', 'Sale|Winfield S|WS|', 'Alford|Lea M|LM|']
D001692:Biological Transport D002478:Cells, Cultured D002696:Chlamydomonas D002923:Cilia D006801:Humans D012038:Regeneration
Axonemal assembly Ciliary regeneration Chlamydomonas Deciliation pH shock