Galactose-functionalized gelatin hydrogels improve the functionality of encapsulated HepG2 cells.
The present study investigates the effect of galactosylated gelatin on encapsulated HepG2 cells. Methacrylamide modified gelatin is evaluated and compared with its galactosylated counterpart with respect to effects on viability, morphological characteristics, proliferation, and the expression of hepatocyte specific markers. The research reveals that further modifications of methacrylamide modified gelatin are possible without affecting the survival of the encapsulated cells (viability of 90%). Moreover, the study demonstrates a clear and long-term (up to 21 d) improvement in hepatocyte specific gene expression when the cells are encapsulated in the galactosylated gelatin. It is concluded that the use of galactosylated gelatin derivates supports the hepatocyte phenotype.
['Gevaert|Elien|E|', 'Billiet|Thomas|T|', 'Declercq|Heidi|H|', 'Dubruel|Peter|P|', 'Cornelissen|Ria|R|']
D000178:Acrylamides D000418:Albumins D015415:Biomarkers D049109:Cell Proliferation D002470:Cell Survival D018914:Cells, Immobilized D005690:Galactose D005780:Gelatin D015870:Gene Expression D056945:Hep G2 Cells D051557:Hepatocyte Nuclear Factor 4 D006801:Humans D020100:Hydrogels D019394:Ki-67 Antigen D011228:Prealbumin
gelatin hydrogels hepatocyte biocompatibility galactose