Long non-coding RNA F11-AS1 inhibits HBV-related hepatocellular carcinoma progression by regulating NR1I3 via binding to microRNA-211-5p.
Long non-coding RNAs (lncRNAs) could regulate growth and metastasis of hepatocellular carcinoma (HCC). In this study, we aimed to investigate the mechanism of lncRNA F11-AS1 in hepatitis B virus (HBV)-related HCC. The relation of lncRNA F11-AS1 expression in HBV-related HCC tissues to prognosis was analysed in silico. Stably HBV-expressing HepG2.2.15 cells were established to explore the regulation of lncRNA F11-AS1 by HBx protein, as well as to study the effects of overexpressed lncRNA F11-AS1 on proliferation, migration, invasion and apoptosis in vitro. Subsequently, the underlying interactions and roles of lncRNA F11-AS1/miR-211-5p/NR1I3 axis in HBV-related HCC were investigated. Additionally, the influence of lncRNA F11-AS1 and miR-211-5p on tumour growth and metastasis capacity of HepG2.2.15 cells were studied on tumour-bearing nude mice. Poor expression of lncRNA F11-AS1 was correlated with poor prognosis in patients with HBV-related HCC, and its down-regulation was caused by the HBx protein. lncRNA F11-AS1 was proved to up-regulate the NR1I3 expression by binding to miR-211-5p. Overexpression of lncRNA F11-AS1 reduced the proliferation, migration and invasion, yet induced apoptosis of HepG2.2.15 cells in vitro, which could be abolished by overexpression of miR-211-5p. Additionally, either lncRNA F11-AS1 overexpression or miR-211-5p inhibition attenuated the tumour growth and metastasis capacity of HepG2.2.15 cells in vivo. Collectively, lncRNA F11-AS1 acted as a modulator of miR-211-5p to positively regulate the expression of NR1I3, and the lncRNA F11-AS1/miR-211-5p/NR1I3 axis participated in HBV-related HCC progression via interference with the cellular physiology of HCC.
['Deng|Yibin|Y|0000-0002-1946-1902', 'Wei|Zhongheng|Z|', 'Huang|Meijin|M|', 'Xu|Guidan|G|', 'Wei|Wujun|W|', 'Peng|Bin|B|', 'Nong|Shunqiang|S|', 'Qin|Houji|H|']
D017209:Apoptosis D001483:Base Sequence D006528:Carcinoma, Hepatocellular D002465:Cell Movement D049109:Cell Proliferation D000090702:Constitutive Androstane Receptor D018450:Disease Progression D005260:Female D015972:Gene Expression Regulation, Neoplastic D056945:Hep G2 Cells D006515:Hepatitis B virus D006801:Humans D008113:Liver Neoplasms D008297:Male D035683:MicroRNAs D008875:Middle Aged D008954:Models, Biological D009361:Neoplasm Invasiveness D009362:Neoplasm Metastasis D011379:Prognosis D011485:Protein Binding D062085:RNA, Long Noncoding D018160:Receptors, Cytoplasmic and Nuclear D015534:Trans-Activators D015854:Up-Regulation D054334:Viral Regulatory and Accessory Proteins
hepatocellular carcinoma microRNA-211-5p hepatitis B virus long non-coding RNA F11-antisense 1 nuclear receptor constitutive androstane receptor