Expression and significance of ASPP2 in squamous carcinoma of esophagus.
To study the significance of apoptosis stimulating protein of P53 2 (ASPP2) expression in esophageal squamous cell carcinoma (ESCC), immunohistochemistry S-P method was used to examine the expression of ASPP2 in 136 cases of ESCC, 35 cases of high grade intraepithelial neoplasia (HGIN), 29 cases of low grade intraepithelial neoplasia (LGIN) and 37 cases of normal esophageal epithelium (NEE). The associations of ASPP2 expression with clinicopathological data and overall survival (OS) were also analyzed. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate ASPP2 expression in a total of 20 matched human ESCC tumor tissues and normal adjacent tissues (NAT). In addition, EC109 cells were treated with cisplatin (CDDP) in vitro for 24 h (the intervention group) and the control group was set up at the same time. Western blot was used to examine the expression of ASPP2 protein between the two groups. The expression of ASPP2 decreased progressively from NEE to LGIN, to HGIN, and to ESCC, and it was related to TNM stage, histological differentiation and lymph node metastasis in ESCC (P < 0.05). ASPP2 was a protective factor of patients with ESCC (P = 0.008). The relative expression of ASPP2 mRNA was markedly downregulated in ESCC compared with the paired NAT (P < 0.01). Western blot results showed that cells in the intervention group could express ASPP2 while there was no expression of ASPP2 in the control group. Taken together, these results indicate that the abnormal expression of ASPP2 may play an important role for development and metastasis in ESCC.
['Liu|Bo|B|', 'Yang|Lv|L|', 'Li|Xiu-Juan|XJ|', 'Li|Rou|R|', 'Sun|Wei|W|', 'Chen|Xiao-Yi|XY|', 'Liu|Jun-Chao|JC|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D051017:Apoptosis Regulatory Proteins D002294:Carcinoma, Squamous Cell D016022:Case-Control Studies D045744:Cell Line, Tumor D049109:Cell Proliferation D002470:Cell Survival D002945:Cisplatin D018450:Disease Progression D004938:Esophageal Neoplasms D000077277:Esophageal Squamous Cell Carcinoma D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D053208:Kaplan-Meier Estimate D008297:Male D008875:Middle Aged D012333:RNA, Messenger
ASPP2 Esophageal squamous cell carcinoma Immunohistochemistry Overall survival EC109