Silencing SOX2 Expression by RNA Interference Inhibits Proliferation, Invasion and Metastasis, and Induces Apoptosis through MAP4K4/JNK Signaling Pathway in Human Laryngeal Cancer TU212 Cells.
SRY (sex determining region Y)-box 2 (SOX2) plays an important role in tumor cell metastasis and apoptosis. Laryngeal squamous cell carcinoma (LSCC), responsible for 1.5% of all cancers, is one of the most common head and neck malignancies. Accumulating evidence shows that SOX2 is overexpressed in several human tumors, including lung cancer, esophageal carcinoma, pancreatic carcinoma, breast cancer, ovarian carcinoma and glioma. Our study aimed to investigate the silencing effects of SOX2 expression using RNA interference (RNAi) on various biological processes in laryngeal cancer TU212 cells, including proliferation, apoptosis, invasion and metastasis. We also studied the involvement of the MAPK/JNK signaling pathway in the biological effects of SOX2 siRNA in TU212 cells. We found that silencing SOX2 decreased the proliferation, migration, and invasion of TU212 cells, and induced apoptosis. This effect of silencing SOX2 could be reversed by silencing MAP4K4. Therefore, we consider SOX2 as a key regulator of the upstream MAP4K4/JNK signaling pathways that could be a potential therapeutic target in the treatment of patients with or prevention of laryngeal cancer.
['Yang|Ning|N|', 'Wang|Yan|Y|', 'Hui|Lian|L|', 'Li|Xiaotian|X|', 'Jiang|Xuejun|X|']
D017209:Apoptosis D002294:Carcinoma, Squamous Cell D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D002999:Clone Cells D018450:Disease Progression D015536:Down-Regulation D004789:Enzyme Activation D015972:Gene Expression Regulation, Neoplastic D006801:Humans D047908:Intracellular Signaling Peptides and Proteins D048031:JNK Mitogen-Activated Protein Kinases D007822:Laryngeal Neoplasms D020935:MAP Kinase Signaling System D009361:Neoplasm Invasiveness D009362:Neoplasm Metastasis D010766:Phosphorylation D017346:Protein Serine-Threonine Kinases D034622:RNA Interference D034741:RNA, Small Interfering D055748:SOXB1 Transcription Factors
proliferation invasion metastasis apoptosis MAP4K4/JNK signaling pathways laryngeal cancer TU212 cells SRY (sex determining region Y)-box 2 (SOX2) siRNA