Dunaliella salina exhibits an antileukemic immunity in a mouse model of WEHI-3 leukemia cells.
Dunaliella salina has been shown to have antioxidant property and induce apoptotic cell death of human cancer cells in vitro. However, there is no information available on D. salina showing an antileukemia effect or immunomodulatory activity in vivo. This study applied D. salina to syngeneic leukemia-implanted mice (BALB/c and WEHI-3) to investigate its immunological and antileukemia properties. Oral administration of D. salina (184.5, 369, and 922.5 mg/kg) inhibited spleen metastasis and prolonged the survival in BALB/c mice that had received an intravenous injection of WEHI-3 cells. The results revealed that D. salina had reduced spleen enlargement in murine leukemia. It had also increased the population and proliferation of T-cells (CD3) and B-cells (CD19) following Con A/LPS treatment on flow cytometry and MTT assay, respectively. Furthermore, D. salina increased the phagocytosis of macrophages and enhanced the cytotoxicity of natural killer cells on flow cytometry and LDH assay. Moreover, D. salina enhanced the levels of interferon-γ and interleukin 2 (IL-2) but reduced the levels of IL-4 and IL-10 in leukemic mice. In conclusion, these results demonstrated that the application of D. salina had beneficial effects on WEHI-3 leukemic mice by prolonging survival via modulating the immune responses.
['Chuang|Wen-Chen|WC|', 'Ho|Yung-Chyuan|YC|', 'Liao|Jiunn-Wang|JW|', 'Lu|Fung-Jou|FJ|']
D000284:Administration, Oral D000818:Animals D000970:Antineoplastic Agents D017209:Apoptosis D001402:B-Lymphocytes D045744:Cell Line, Tumor D049109:Cell Proliferation D003208:Concanavalin A D007371:Interferon-gamma D016753:Interleukin-10 D007376:Interleukin-2 D015847:Interleukin-4 D007694:Killer Cells, Natural D008264:Macrophages D051379:Mice D008807:Mice, Inbred BALB C D008934:Mitogens D009929:Organ Size D010587:Phagocytosis D013154:Spleen D013601:T-Lymphocytes D016823:Volvocida
Dunaliella salina antileukemia immune responses