Measurement of intact quantal packet of transmitters released from single nerve terminal by loose-patch amperometry.
Neuronal information is majorly encoded chemically at synapses and the elementary unit of synaptic transmission is the contents of neurotransmitter released from single vesicle. However, the contents of quantal neurotransmitter have never been precisely estimated at synapses, which largely prevent our understanding the nature of quantal neurotransmitter release and its impact on neuronal information processing. In order to break through the technical bottleneck of precisely counting quantal neurotransmitter molecules, we developed a new approach in combination of electrophysiology and electrochemistry to measure intact quantal content of single vesicles. An etched submicro-carbon fiber electrode for electrochemical detection was designed to be enclosed in an electrophysiologically used glass pipette. The glass pipette allowed the electrochemical electrode to access the release site, and amperometric recordings were made within the enclosed space at the electrophysiological loose-patch mode. Our study showed that the intact quantal release could be successfully detected at the dopaminergic varicosities by this loose-patch amperometric measurement in real time with negligible leakage.
['Chen|Peihua|P|', 'Shen|Xuefeng|X|', 'Zhao|Shuainan|S|', 'Liu|Zili|Z|', 'Zhu|Qianwen|Q|', 'Zhu|Tao|T|', 'Zhang|Shuli|S|', 'Li|Yi|Y|', 'Mao|Lanqun|L|', 'Sun|Jianyuan|J|']
D015374:Biosensing Techniques D009474:Neurons D018377:Neurotransmitter Agents D013569:Synapses D009435:Synaptic Transmission
Nerve terminals Amperometry Neurotransmitters Quantal release Loose patch