Long Noncoding RNA LINC00634 Functions as an Oncogene in Esophageal Squamous Cell Carcinoma Through the miR-342-3p/Bcl2L1 Axis.
Many long noncoding RNAs reportedly have tumor suppressive roles or are oncogenic in esophageal cancer. We have previously performed a chip-based expression analysis of primary esophageal cancer tissues and found that the expression of LINC00634 in these tissues was higher than that in nontumor tissues. Quantitative real-time-polymerase chain reaction, cell counting kit-8, flow cytometry, caspase3/7 assay, dual-luciferase reporter assay, and restore assay were used to detect the proliferative and apoptotic effects of LINC00634 in esophageal cancer cells. The results showed that the expression of LINC00634 in these tissues was higher than that in nontumor tissues and associated with tumor-node-metastasis (TNM) stage of patients. Knockdown of LINC00634 decreased cell viability and increased cell apoptosis levels in EC9706 and EC1 cells. LINC00634 could target Bcl2L1 through miR-342-3p. In this study, we show that LINC00634 is upregulated in esophageal cancer. We also show that the knockdown of LINC00634 decreased cell viability and increased cell apoptosis levels in EC9706 and EC1 cells through the miR-342-3p/Bcl2L1 axis.
['Zhang|Xiaohong|X|0000-0002-1737-1225', 'Feng|Yinman|Y|', 'Gao|Yanli|Y|', 'Hu|Jun|J|']
D017209:Apoptosis D014408:Biomarkers, Tumor D049109:Cell Proliferation D004938:Esophageal Neoplasms D000077277:Esophageal Squamous Cell Carcinoma D015972:Gene Expression Regulation, Neoplastic D006801:Humans D035683:MicroRNAs D011379:Prognosis D062085:RNA, Long Noncoding D014407:Tumor Cells, Cultured D051020:bcl-X Protein
miR-342-3p Bcl2L1 esophageal cancer lncRNA