An Approach to Identify and Characterize a Subunit Candidate Shigella Vaccine Antigen.
Shigellosis remains a serious issue throughout the developing countries, particularly in children under the age of 5. Numerous strategies have been tested to develop vaccines targeting shigellosis; unfortunately despite several years of extensive research, no safe, effective, and inexpensive vaccine against shigellosis is available so far. Here, we illustrate in detail an approach to identify and establish immunogenic outer membrane proteins from Shigella flexneri 2a as subunit vaccine candidates.
['Pore|Debasis|D|', 'Chakrabarti|Manoj K|MK|']
D000818:Animals D000942:Antigens, Bacterial D001402:B-Lymphocytes D001425:Bacterial Outer Membrane Proteins D003001:Cloning, Molecular D005260:Female D015870:Gene Expression D008213:Lymphocyte Activation D017737:Macrophages, Peritoneal D008297:Male D051379:Mice D011994:Recombinant Proteins D017421:Sequence Analysis D012760:Shigella D022281:Shigella Vaccines D013601:T-Lymphocytes D022223:Vaccines, Subunit
Outer membrane proteins Shigella flexneri 2a B cell Macrophages Protective immune response T cell