Development of a clickable activity-based protein profiling (ABPP) probe for agmatine deiminases.
Agmatine deiminases (AgDs) catalyze the hydrolytic conversion of agmatine (decarboxylated arginine) to N-carbamoylputrescine with concomitant release of ammonia. These enzymes, which are encoded by some pathogenic bacterial species, confer a competitive survival advantage by virtue of energy production and acid tolerance through agmatine catabolism. Herein we report the development of a clickable activity-based protein profiling (ABPP) probe that targets the AgD encoded by Streptococcus mutans with high selectivity and sensitivity.
['Marchenko|Mikhail|M|', 'Thomson|Andrew|A|', 'Ellis|Terri N|TN|', 'Knuckley|Bryan|B|', 'Causey|Corey P|CP|']
D055162:Biocatalysis D057930:Click Chemistry D015195:Drug Design D006867:Hydrolases D007700:Kinetics D015335:Molecular Probes D015394:Molecular Structure D013295:Streptococcus mutans D013379:Substrate Specificity
Activity-based protein profiling Agmatine deiminase Agmatine catabolism ABPP probe