Simultaneous determination of tedizolid and linezolid in rat plasma by ultra performance liquid chromatography tandem mass spectrometry and its application to a pharmacokinetic study.
A sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine tedizolid and linezolid in rat plasma simultaneously. Chromatographic separation was carried out on an Acquity UPLC BEH C18 column and mass spectrometric analysis was performed using a XEVO TQD triple quadruple mass spectrometer coupled with an electrospray ionization (ESI) source in the positive ion mode. Multiple reaction monitoring (MRM) mode was used for quantification using target fragment ions m/z 371.4→343.2 for tedizolid, and m/z 338.3→56.1 for linezolid. This assay method has been fully validated in terms of selectivity, linearity, recovery and matrix effect, accuracy, precision and stability. The linearity of this method was found to be within the concentration range of 5-5000ng/mL for tedizolid, and 10-10,000ng/mL for linezolid in rat plasma, respectively. Only 3.0min was needed for an analytical run. This assay was used to support a preclinical study where multiple oral doses were administered to rats to investigate the pharmacokinetics of tedizolid and linezolid.
['Yu|Hua-chen|HC|', 'Pan|Chen-wei|CW|', 'Xie|Qi-peng|QP|', 'Zheng|Yi|Y|', 'Hu|Yue-zheng|YZ|', 'Lin|Yi-mu|YM|']
D000818:Animals D002851:Chromatography, High Pressure Liquid D057230:Limit of Detection D016014:Linear Models D000069349:Linezolid D008297:Male D023303:Oxazolidinones D051381:Rats D017207:Rats, Sprague-Dawley D015203:Reproducibility of Results D053719:Tandem Mass Spectrometry D013777:Tetrazoles
Tedizolid Linezolid Rat plasma UPLC-MS/MS