Antibacterial action of lactoferricin B like peptide against Escherichia coli: reactive oxygen species-induced apoptosis-like death.
AIMS
Emergence and rapid dissemination of antibiotic-resistant bacteria is becoming a severe problem to public health. The search for antimicrobial substitutes for antibiotics is necessary. Lactoferricin B like peptide (LBLP) is a 23-mer antimicrobial peptide (AMP), derived from the big centipede Scolopendra subspinipes mutilans. Although its antifungal effect and its mechanism have been reported, the antibacterial activity has not yet been elucidated.


METHOD AND RESULTS
In this study, we investigated antibacterial activity of LBLP and its mode of action. LBLP showed potent antibacterial effect against pathogenic bacteria Escherichia coli and did not show haemolytic activity against human erythrocyte. The general antimicrobial mechanism of AMP is to disrupt the cell membrane, however, LBLP exerted its antibacterial activity by causing apoptosis-like death through reactive oxygen species (ROS) generation. LBLP-treated E. coli cells exhibited hallmarks of apoptosis, such as membrane depolarization, DNA fragmentation, caspase-like protein activation and phosphatidylserine exposure. These apoptotic features were attenuated by pretreatment of NAC, a representative ROS scavenger.


CONCLUSIONS
These results demonstrate that LBLP exerted its antibacterial activity by generating ROS and inducing apoptosis-like death in E. coli. LBLP is not membrane destructive per se, but essentially a metabolic inhibitor.


SIGNIFICANCE AND IMPACT OF THE STUDY
Lactoferricin B like peptide is potential candidate to replace conventional antibiotics that are less effective because of its unique properties.
['Lee|B|B|', 'Hwang|J S|JS|', 'Lee|D G|DG|https://orcid.org/0000-0002-7972-433X']
D000818:Animals D000900:Anti-Bacterial Agents D017209:Apoptosis D001181:Arthropods D004926:Escherichia coli D006801:Humans D007781:Lactoferrin D010455:Peptides D017382:Reactive Oxygen Species
lactoferricin B like peptide antimicrobial peptide antibacterial activity reactive oxygen species bacterial apoptosis-like death