High-throughput method for the quantitation of metabolites and co-factors from homocysteine-methionine cycle for nutritional status assessment.
AIM
There is increasing interest in the profiling and quantitation of methionine pathway metabolites for health management research. Currently, several analytical approaches are required to cover metabolites and co-factors.


RESULTS
We report the development and the validation of a method for the simultaneous detection and quantitation of 13 metabolites in red blood cells. The method, validated in a cohort of healthy human volunteers, shows a high level of accuracy and reproducibility.


CONCLUSION
This high-throughput protocol provides a robust coverage of central metabolites and co-factors in one single analysis and in a high-throughput fashion. In large-scale clinical settings, the use of such an approach will significantly advance the field of nutritional research in health and disease.
['Da Silva|Laeticia|L|', 'Collino|Sebastiano|S|', 'Cominetti|Ornella|O|', 'Martin|Francois-Pierre|FP|', 'Montoliu|Ivan|I|', 'Moreno|Sergio Oller|SO|', 'Corthesy|John|J|', 'Kaput|Jim|J|', 'Kussmann|Martin|M|', 'Monteiro|Jacqueline Pontes|JP|', 'Guiraud|Seu Ping|SP|']
D000293:Adolescent D002648:Child D002851:Chromatography, High Pressure Liquid D015331:Cohort Studies D004912:Erythrocytes D005260:Female D057166:High-Throughput Screening Assays D006710:Homocysteine D006801:Humans D057230:Limit of Detection D008297:Male D053858:Metabolic Networks and Pathways D008715:Methionine D009752:Nutritional Status D015203:Reproducibility of Results D053719:Tandem Mass Spectrometry
methionine pathway red blood cells UPLC–MS/MS high throughput one-carbon metabolism