Electric-field driven assembly of live bacterial cell microarrays for rapid phenotypic assessment and cell viability testing.
Microarray technology to isolate living cells using external fields is a facile way to do phenotypic analysis at the cellular level. We have used alternating current dielectrophoresis (AC-DEP) to drive the assembly of live pathogenic Salmonella typhi (S.typhi) and Escherichia coli (E.coli) bacteria into miniaturized single cell microarrays. The effects of voltage and frequency were optimized to identify the conditions for maximum cell capture which gave an entrapment efficiency of 90% in 60 min. The chip was used for calibration-free estimation of cellular loads in binary mixtures and further applied for rapid and enhanced testing of cell viability in the presence of drug via impedance spectroscopy. Our results using a model antimicrobial sushi peptide showed that the cell viability could be tested down to 5 μg/mL drug concentration under an hour, thus establishing the utility of our system for ultrafast and sensitive detection.
['Goel|Meenal|M|', 'Verma|Abhishek|A|', 'Gupta|Shalini|S|']
D000900:Anti-Bacterial Agents D015374:Biosensing Techniques D058266:Dielectric Spectroscopy D004867:Equipment Design D004926:Escherichia coli D004927:Escherichia coli Infections D006801:Humans D050296:Microbial Viability D008839:Microelectrodes D012485:Salmonella typhi D046888:Tissue Array Analysis D014435:Typhoid Fever
Cell viability Dielectrophoresis Impedance spectroscopy Cell counting Cellular microarray