Nasopharyngeal Carriage, Capsular and Molecular Serotyping and Antimicrobial Susceptibility of Streptococcus pneumoniae among Asymptomatic Healthy Children in Egypt.
Streptococcus pneumoniae is a major cause of childhood morbidity and mortality worldwide with increasing antimicrobial resistance. 600 randomly chosen asymptomatic healthy children aged 2-60 months attending Alexandria University Children's Hospital were evaluated for prevalence of nasopharyngeal (NP) carriage of S. pneumoniae. Prevalence of NP carriage was 29.2% (n = 175/600) Capsular serotyping was done using Quellung reaction. Vaccine covered serotypes (VST) represented 67.4% while non-vaccine serotypes (NVST) were 32.6%. The most common VST isolated were 19F (24.6%), 6B (14.3%) and 6A (10.9%). Confirmation of serotyping was performed by multiplex PCR which showed 100% concordance with the Quellung reaction. Antimicrobial susceptibility testing showed penicillin non-susceptibility of 15% (using non-meningitis penicillin MIC breakpoints) and 55% (using meningitis penicillin MIC breakpoints). Highest resistance was found in sulphamethoxazole-trimethoprim (55%), tetracyclins (49%), erythromycin (40%) and clindamycin (25%). This study revealed the epidemiological importance to evaluate regularly the prevalence, serotypes and the increasing antimicrobial resistance of S. pneumoniae in the community.
['El-Nawawy|Ahmed A|AA|', 'Hafez|Soad F|SF|', 'Meheissen|Marwa A|MA|', 'Shahtout|Nehal M|NM|', 'Mohammed|Essam E|EE|']
D000890:Anti-Infective Agents D002353:Carrier State D002648:Child D024881:Drug Resistance, Bacterial D004534:Egypt D005260:Female D006801:Humans D007223:Infant D008297:Male D008826:Microbial Sensitivity Tests D060885:Multiplex Polymerase Chain Reaction D009305:Nasopharynx D011008:Pneumococcal Infections D022242:Pneumococcal Vaccines D015995:Prevalence D012703:Serotyping D013296:Streptococcus pneumoniae
nasopharyngeal carriage serotype antimicrobial susceptibility Streptococcus pneumoniae Quellung multiplex PCR