Identification of quantitative trait locus (QTL) linked to dorsal fin length from preliminary linkage map of molly fish, Poecilia sp.
A preliminary linkage map was constructed by applying backcross and testcross strategy using microsatellite (SSR) markers developed for Xiphophorus and Poecilia reticulata in ornamental fish, molly Poecilia sp. The linkage map having 18 SSR loci consisted of four linkage groups that spanned a map size of 516.1cM. Association between genotypes and phenotypes was tested in a random fashion and QTL for dorsal fin length was found to be linked to locus Msb069 on linkage group 2. Coincidentally, locus Msb069 was also reported as putative homologue primer pairs containing SSRs repeat motif which encoded hSMP-1, a sex determining locus. Dorsal fin length particularly in males of Poecilia latipinna is an important feature during courtship display. Therefore, we speculate that both dorsal fin length and putative hSMP-1 gene formed a close proximity to male sexual characteristics.
['Keong|Bun Poh|BP|', 'Siraj|Siti Shapor|SS|', 'Daud|Siti Khalijah|SK|', 'Panandam|Jothi Malar|JM|', 'Rahman|Arina Nadia Abdul|AN|']
D058500:Animal Fins D000818:Animals D002874:Chromosome Mapping D005260:Female D008040:Genetic Linkage D008297:Male D009929:Organ Size D011036:Poecilia D011110:Polymorphism, Genetic D040641:Quantitative Trait Loci
QTL Dorsal fin length Linkage map Backcross Microsatellites SSRs sp. Linkage group Poecilia latipinna BC Base pairs Centimorgan Coefficient of determination DFL Degree celsius F(1) F(2) First filial generation LG LOD Logarithm of odds Male courtship display Microlitre Minute Number P Probability Quantitative Trait Loci Quantitative trait loci R(2) Second filial generation Species USA United States of America bp cM min n °C μl