A novel protein-based autologous topical serum for skin regeneration.
BACKGROUND
As skin ages, a functional decrement occurs. To avoid future vulnerability to dermatologic diseases, an optimal cutaneous regeneration is mandatory. Biological therapies based on blood-derived autologous proteins are gaining attention of scientists and dermatologists.


OBJECTIVES
A novel 100% autologous topical serum has been developed using plasma rich in growth factors technology. The physicochemical characterization and the biologic potential of the novel formulation have been studied.


METHODS
Rheological and mechanical properties and the biological capacity of the formulation were characterized. Human dermal fibroblast culture and 3D organotypic skin explants were used as in vitro and ex vivo cutaneous models, respectively.


RESULTS
The autologous topical serum presented an optimal spreadability index and appropriate shear thinning behavior that allowed an easy handling and rapid integration within the cutaneous tissue. The formulation has a high growth factor load with the ability to progressively penetrate into the dermal/epidermal layers of the skin. It is biocompatible and promotes cell proliferation and chemotactic activity. The autologous topical serum promotes the biosynthetic activity of cells by the stimulation of collagen and hyaluronic acid expression.


CONCLUSIONS
These findings present an in situ and easy to prepare autologous topical serum based on the patient's own blood with physicochemical and bioactive properties that may be used for skin regeneration purposes.
['Anitua|Eduardo|E|https://orcid.org/0000-0002-8386-5303', 'Troya|María|M|', 'Pino|Ander|A|https://orcid.org/0000-0003-3926-4989']
D000279:Administration, Cutaneous D001685:Biological Factors D001804:Blood Transfusion, Autologous D002478:Cells, Cultured D003094:Collagen D004353:Drug Evaluation, Preclinical D005347:Fibroblasts D006801:Humans D006820:Hyaluronic Acid D010982:Platelet-Derived Growth Factor D061251:Primary Cell Culture D012038:Regeneration D012867:Skin D012869:Skin Absorption D015595:Skin Aging D046509:Tissue Culture Techniques
skin regeneration growth factors platelet-rich plasma topical application