Rapid screening of marine bacterial symbionts using MALDI-TOF MS.
Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, including Vibrio, Bacillus, Pseudovibrio, Alteromonas and Ruegeria. MALDI-TOF distance similarity scores ≥ 60% and ≥ 70% correspond to ≥ 98.7% 16S rRNA gene sequence similarity and ≥ 95% pyrH gene sequence similarity, respectively. MALDI-TOF MS is a useful tool for Vibrio species groups' identification.
['Vidal|Livia M R|LMR|', 'Venas|Tainá M|TM|', 'Gonçalves|Aline R P|ARP|', 'Mattsson|Hannah K|HK|', 'Silva|Raphael V P|RVP|', 'Nóbrega|Maria S|MS|', 'Azevedo|Gustavo P R|GPR|', 'Garcia|Gizele D|GD|', 'Tschoeke|Diogo A|DA|', 'Vieira|Verônica V|VV|', 'Thompson|Fabiano L|FL|', 'Thompson|Cristiane C|CC|http://orcid.org/0000-0001-9510-3675']
D004783:Environmental Microbiology D008386:Marine Biology D012336:RNA, Ribosomal, 16S D012623:Seawater D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization D013559:Symbiosis D014733:Vibrio
Marine bacterial symbionts MALDI-TOF Screening tool Vibrio pyrH gene