Candidate chemosensory genes in the Stemborer Sesamia nonagrioides.
The stemborer Sesamia nonagrioides is an important pest of maize in the Mediterranean Basin. Like other moths, this noctuid uses its chemosensory system to efficiently interact with its environment. However, very little is known on the molecular mechanisms that underlie chemosensation in this species. Here, we used next-generation sequencing (454 and Illumina) on different tissues from adult and larvae, including chemosensory organs and female ovipositors, to describe the chemosensory transcriptome of S. nonagrioides and identify key molecular components of the pheromone production and detection systems. We identified a total of 68 candidate chemosensory genes in this species, including 31 candidate binding-proteins and 23 chemosensory receptors. In particular, we retrieved the three co-receptors Orco, IR25a and IR8a necessary for chemosensory receptor functioning. Focusing on the pheromonal communication system, we identified a new pheromone-binding protein in this species, four candidate pheromone receptors and 12 carboxylesterases as candidate acetate degrading enzymes. In addition, we identified enzymes putatively involved in S. nonagrioides pheromone biosynthesis, including a ∆11-desaturase and different acetyltransferases and reductases. RNAseq analyses and RT-PCR were combined to profile gene expression in different tissues. This study constitutes the first large scale description of chemosensory genes in S. nonagrioides.
['Glaser|Nicolas|N|', 'Gallot|Aurore|A|', 'Legeai|Fabrice|F|', 'Montagné|Nicolas|N|', 'Poivet|Erwan|E|', 'Harry|Myriam|M|', 'Calatayud|Paul-André|PA|', 'Jacquin-Joly|Emmanuelle|E|']
D000595:Amino Acid Sequence D000818:Animals D001483:Base Sequence D019295:Computational Biology D017931:DNA Primers D020869:Gene Expression Profiling D017344:Genes, Insect D059014:High-Throughput Nucleotide Sequencing D016013:Likelihood Functions D008957:Models, Genetic D008969:Molecular Sequence Data D009036:Moths D010675:Pheromones D010802:Phylogeny D043563:Receptors, Pheromone D020133:Reverse Transcriptase Polymerase Chain Reaction D012903:Smell D003313:Zea mays
next-generation sequencing transcriptome chemosensory receptors pheromone biosynthesis Sesamia nonagrioides.