A gelatin based antioxidant enriched biomaterial by grafting and saturation: towards sustained drug delivery from antioxidant matrix.
Proteins grafted with antioxidant molecules have drawn much attention due to their increased life time and biocompatibility. When protein macromolecules are cross linked chemically and physically with antioxidant molecules, they can act as antioxidant biomaterials as well as scaffolds to release the antioxidant molecules by diffusion. In our work, we have attempted to release catechin molecules from the matrix of glutathione grafted gelatin. Conjugation of glutathione and cross linkage was done by carbodiimide method to achieve smaller pores in the gelatin matrix and the characterization was performed by means of FTIR-ATR and calorimetric analyses. The glutathione grafted gelatin (GGSH) has been shown to have more thermal stability and pores with lesser radii than blank gelatin (bGEL). Free radical scavenging activity of GGSH was also found to be more than that of bGEL. Catechin was added to GGSH and bGEL by physical blending in order to achieve short term release of antioxidant molecules. CD spectra revealed that significant conformational changes occurred in secondary structure of gelatin upon interaction with catechin. Slower rate of catechin release from GGSH reflected the influence of cross linkage and physical interactive forces on the drug release properties. We conclude that the mixture of catechin with GGSH can be a potent antioxidant biomaterial releasing catechin at slower rate than the mixture of catechin with bGEL.
['Raja|I Selestin|IS|', 'Fathima|Nishter Nishad|NN|']
D000975:Antioxidants D001672:Biocompatible Materials D001713:Biphenyl Compounds D002234:Carbodiimides D002392:Catechin D003432:Cross-Linking Reagents D003692:Delayed-Action Preparations D004058:Diffusion D065546:Drug Liberation D005780:Gelatin D005978:Glutathione D010851:Picrates D055550:Protein Stability D017433:Protein Structure, Secondary
Gelatin Catechin Glutathione Circular dichroism Differential scanning calorimetry