Identification and Characterization of Diploid and Tetraploid in Platycodon grandiflorum.
Platycodon grandiflorum (PG), a species of herbaceous flowering perennial plant of the family Campanulaceae, has been used as a traditional oriental medicine for bronchitis, asthma, pulmonary tuberculosis, diabetes, hepatic fibrosis, bone disorders and many others similar diseases and as a food supplement. For the primary profiling of PG gas chromatography coupled with high resolution - time of flight mass spectrometry (GC/HR-TOF MS) was used as an analytical tool. A comparison of optimal extraction of metabolites was carried out with a number of solvents [hexane, methylene chloride, methanol, ethanol, methanol: ethanol (70:30, v:v)]. In extracts with methanol: ethanol (70:30 v:v) were detected higher amounts of metabolites than with other solvents. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) plots showed significant differences between the diploid and tetraploid metabolite profiles. Extracts of tetraploid showed higher amounts of amino acids, while extracts of diploid contained more organic acids and sugars. Graphical Abstract ᅟ.
['Shin|Jeoung-Hwa|JH|', 'Ahn|Yun Gyong|YG|', 'Jung|Ju-Hee|JH|', 'Woo|Sun-Hee|SH|', 'Kim|Hag-Hyun|HH|', 'Gorinstein|Shela|S|', 'Boo|Hee-Ock|HO|']
D000596:Amino Acids D002241:Carbohydrates D002264:Carboxylic Acids D004171:Diploidy D008401:Gas Chromatography-Mass Spectrometry D055432:Metabolomics D010936:Plant Extracts D031238:Platycodon D025341:Principal Component Analysis D057891:Tetraploidy
Diploid Tetraploid Platycodon grandiflorum (PG) Gas chromatography/high resolution time of flight mass spectrometry (GC/HR-TOF MS) Partial least-squares discriminant analysis (OPLS-DA)