Metabolic profiles of planktonic and biofilm cells of Candida orthopsilosis.
AIM
This study aims to understand which Candida orthopsilosis protein aids fungus adaptation upon its switching from planktonic growth to biofilm.


MATERIALS & METHODS
Ion mobility separation within mass spectrometry analysis combination were used.


RESULTS
Proteins mapped for different biosynthetic pathways showed that selective ribosome autophagy might occur in biofilms. Glucose, used as a carbon source in the glycolytic flux, changed to glycogen and trehalose.


CONCLUSION
Candida orthopsilosis expresses proteins that combine a variety of mechanisms to provide yeasts with the means to adjust the catalytic properties of enzymes. Adjustment of the enzymes helps modulate the biosynthesis/degradation rates of the available nutrients, in order to control and coordinate the metabolic pathways that enable cells to express an adequate response to nutrient availability.
['Pires|Regina Helena|RH|', 'Cataldi|Thaís Regiani|TR|', 'Franceschini|Livia Maria|LM|', 'Labate|Mônica Veneziano|MV|', 'Fusco-Almeida|Ana Marisa|AM|', 'Labate|Carlos Alberto|CA|', 'Palma|Mario Sérgio|MS|', 'Soares Mendes-Giannini|Maria José|MJ|']
D000596:Amino Acids D018441:Biofilms D002175:Candida D002244:Carbon D002952:Citric Acid Cycle D005656:Fungal Proteins D015966:Gene Expression Regulation, Fungal D063990:Gene Ontology D005947:Glucose D006003:Glycogen D006019:Glycolysis D064688:Metabolic Flux Analysis D053858:Metabolic Networks and Pathways D055442:Metabolome D005810:Multigene Family D010933:Plankton D020543:Proteome D014199:Trehalose
biofilms Candida orthopsilosis metabolic pathways proteome