Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3.
Cryopreservation of shoot tips facilitates long-term storage of plant genetic resources which can otherwise only be propagated vegetatively. The vitrification approach using the cryoprotectant plant vitrification solution 3 (PVS3, 50% sucrose and 50% glycerol) is easy to handle, has shown to produce high regrowth percentages in a number of potato, mint, garlic, and shallot accessions, and is, thus, highly suitable for routine cryopreservation of plant genetic resources. In the current chapter, the vitrification procedure is described for potato, mint, garlic, and shallot and includes details about modifications for the different plant species. Special emphasis is given on the preparation of the different culture media, solutions, the culture conditions prior and post-cryopreservation, and the preparation of the shoot tips from different sources. Furthermore, protocols to introduce plants into in vitro culture and methods to estimate cryopreservation success are provided.
['Senula|Angelika|A|', 'Nagel|Manuela|M|']
D018929:Cell Culture Techniques D049109:Cell Proliferation D002478:Cells, Cultured D015925:Cryopreservation D003451:Cryoprotective Agents D005737:Garlic D005990:Glycerol D027541:Mentha D018520:Plant Shoots D027781:Shallots D011198:Solanum tuberosum D013395:Sucrose
Long-term storage Plant genetic resources Cryoprotection PVS3 Tissue culture Ultra-low temperature