Effect of bisphenols on telomerase expression and activity in breast cancer cell lines.
Bisphenol A (BPA), a monomer of polycarbonates and resins, was shown to induce the expression of telomerase enzyme which has been associated with breast cancer development and progression. However, the effects of BPA analogues, bisphenol F (BPF) and bisphenol S (BPS) on telomere-linked pathway have not been evaluated. Herein, MCF-7 (estrogen receptor (ER)-positive) and MDA-MB-231 (ER-negative) cells were treated with BPA, BPF and BPS ± estrogen receptor inhibitor (ERI), for 24 and/or 48 h. RNA expression and enzymatic activity of telomerase were measured using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and telomeric repeat amplification protocol (TRAP); respectively. Relative telomere length (RTL) was also measured using quantitative PCR. After 24 h, the three bisphenols resulted in a 2-3 folds increase in expression and activity of telomerase in MCF-7 but not in MDA-MB-231 cells, and this increase was prevented upon co-treatment with ERI. The observed increase in the expression and activity of telomerase after 24 h of treatment with bisphenols was associated with differential and modest ER-dependent lengthening in RTL at 48 h. Our results show that telomerase potentially mediates the effects of the three bisphenols in ER-positive breast carcinoma. Hence, further investigation is warranted to elucidate the telomerase-linked pathways that could underlie bisphenol-related effects.
['Awada|Zainab|Z|', 'Nasr|Rihab|R|', 'Akika|Reem|R|', 'Ghantous|Akram|A|', 'Hou|Lifang|L|', 'Zgheib|Nathalie K|NK|']
D001559:Benzhydryl Compounds D001943:Breast Neoplasms D045744:Cell Line, Tumor D049109:Cell Proliferation D002470:Cell Survival D005260:Female D006801:Humans D061986:MCF-7 Cells D010636:Phenols D013450:Sulfones D019098:Telomerase D059505:Telomere Homeostasis
Bisphenols Telomerase MCF-7 Estrogen receptor MDA-MB-231 Telomere