Analysis of Ethylene Receptors: Ethylene-Binding Assays.
Plant ethylene receptors bind ethylene with high affinity. Most of the characterization of ethylene binding to the receptors has been carried out using a radioligand-binding assay on functional receptors expressed in yeast. In this chapter, we describe methods for expressing ethylene receptors in yeast and conducting ethylene-binding assays on intact yeast and yeast membranes. The ethylene-binding assays can be modified to analyze ethylene binding to intact plants and other organisms as well as membranes isolated from any biological source.
['Binder|Brad M|BM|', 'Schaller|G Eric|GE|']
D001681:Biological Assay D002462:Cell Membrane D005030:Ethylenes D007553:Isotope Labeling D008024:Ligands D010940:Plant Proteins D011485:Protein Binding D011956:Receptors, Cell Surface D015003:Yeasts
Ethylene receptor Ethylene binding Radioligand Yeast expression