Exosome-formed synthetic microRNA-143 is transferred to osteosarcoma cells and inhibits their migration.
MicroRNAs (miRNAs) have emerged as potential anticancer agents, but their clinical application is limited by the lack of an effective delivery system to tumors. Exosomes are small vesicles that play important roles in intercellular communication. Here, we show that synthetic miR-143 introduced into cells is released enveloped in exosomes and that the secreted exosome-formed miR-143 is transferred to osteosarcoma cells. The delivery of exosome-formed miR-143 significantly reduced the migration of osteosarcoma cells. The delivery efficiency of exosome-formed miR-143 was less than that achieved with lipofection, but the migratory potential of osteosarcoma cells was similarly inhibited after both strategies. Our results suggest that exosomes can deliver synthetic miR-143 and are a potentially efficient and functional delivery system.
['Shimbo|Keisuke|K|', 'Miyaki|Shigeru|S|', 'Ishitobi|Hiroyuki|H|', 'Kato|Yoshio|Y|', 'Kubo|Tadahiko|T|', 'Shimose|Shoji|S|', 'Ochi|Mitsuo|M|']
D045744:Cell Line, Tumor D002465:Cell Movement D055354:Exosomes D006801:Humans D059630:Mesenchymal Stem Cells D035683:MicroRNAs D009362:Neoplasm Metastasis D012516:Osteosarcoma D014162:Transfection
Osteosarcoma cells Delivery system Exosome miR-143