Glycine confers neuroprotection through PTEN/AKT signal pathway in experimental intracerebral hemorrhage.
Glycine has been shown to protect against ischemic stroke through various mechanisms. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) which antagonize Akt-dependent cell survival has been linked to neuronal damage. However, whether glycine has a neuroprotective property in intracerebral hemorrhage (ICH) was unknown. This study aimed to determine the protective effect of glycine in rats ICH. Adult male Sprague-Dawley (SD) rats were subjected to left striatum infusion of autologous blood. ICH animals received glycine (0.2-3 mg/kg, icv) at 1 h after ICH with or without pre-injection of Akt Inhibitor IV (100 μM, 2 μl, icv) 0.5 h prior to glycine treatment. Our results showed that in the perihematomal area PTEN was up-regulated in the early stage after ICH. However, glycine treatment decreased PTEN protein level and increased the phosphorylation level of AKT (p-AKT) in the perihematomal area. With the administration of glycine, neuronal death was significantly reduced and Evans blue leakage was alleviated as well as the brain edema after ICH. Moreover, hematoma volume was decreased and neurobehavioral outcome was improved. Nevertheless, Akt Inhibitor IV abolished the neuroprotective effects of glycine after ICH. Together, our findings demonstrate, for the first time, the protective role of glycine on ICH rats, and suggest that the neuroprotective effect of glycine was mediated through PTEN/Akt signal pathway.
['Zhao|Dan|D|', 'Chen|Juan|J|', 'Zhang|Ya|Y|', 'Liao|Hua-Bao|HB|', 'Zhang|Zhi-Feng|ZF|', 'Zhuang|Yang|Y|', 'Pan|Meng-Xian|MX|', 'Tang|Jun-Chun|JC|', 'Liu|Rui|R|', 'Lei|Yang|Y|', 'Wang|Shu|S|', 'Qin|Xing-Ping|XP|', 'Feng|Yu-Gong|YG|', 'Chen|Yun|Y|', 'Wan|Qi|Q|']
D000818:Animals D001562:Benzimidazoles D052160:Benzothiazoles D001929:Brain Edema D016923:Cell Death D002543:Cerebral Hemorrhage D004195:Disease Models, Animal D005998:Glycine D008297:Male D009474:Neurons D000066829:Neuroprotection D018696:Neuroprotective Agents D051059:PTEN Phosphohydrolase D010766:Phosphorylation D051057:Proto-Oncogene Proteins c-akt D051381:Rats D017207:Rats, Sprague-Dawley D015398:Signal Transduction
Glycine Neuroprotection Intracerebral hemorrhage Phosphatase and tensin homolog deleted on chromosome 10