Investigation of temperature effect on cell mechanics by optofluidic microchips.
Here we present the results of a study concerning the effect of temperature on cell mechanical properties. Two different optofluidic microchips with external temperature control are used to investigate the temperature-induced changes of highly metastatic human melanoma cells (A375MC2) in the range of ~0 - 35 °C. By means of an integrated optical stretcher, we observe that cells' optical deformability is strongly enhanced by increasing cell and buffer-fluid temperature. This finding is supported by the results obtained from a second device, which probes the cells' ability to be squeezed through a constriction. Measured data demonstrate a marked dependence of cell mechanical properties on temperature, thus highlighting the importance of including a proper temperature-control system in the experimental apparatus.
['Yang|Tie|T|', 'Nava|Giovanni|G|', 'Minzioni|Paolo|P|', 'Veglione|Manuela|M|', 'Bragheri|Francesca|F|', 'Lelii|Francesca Demetra|FD|', 'Vazquez|Rebeca Martinez|RM|', 'Osellame|Roberto|R|', 'Cristiani|Ilaria|I|']
(130.3120) Integrated optics devices (140.6810) Thermal effects (170.1530) Cell analysis (170.4520) Optical confinement and manipulation