Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540).
In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A620/A280). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A620/A280) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD).
['Kumar|Devendra|D|', 'Dhar|Dolly Wattal|DW|', 'Pabbi|Sunil|S|', 'Kumar|Neeraj|N|', 'Walia|Suresh|S|']
Spirulina Phycocyanin DEAE-Cellulose-11