circMET promotes NSCLC cell proliferation, metastasis, and immune evasion by regulating the miR-145-5p/CXCL3 axis.
In recent years, circular RNAs (circRNAs) have been increasingly reported to play a crucial role in the proliferation, migration, and invasion of non-small-cell lung cancer (NSCLC) cells. However, the circRNA MET (circMET) oncogenic mechanism that drives NSCLC development and progression remains largely unknown. In this study, the present results demonstrated that circMET expression was significantly higher in NSCLC tissues than in peritumoral tissues using quantitative real-time polymerase chain reaction. Notably, NSCLC patients with a large tumor diameter, poor differentiation and lymphatic metastasis had high RNA levels of circMET. Moreover, high circMET expression served as an independent risk factor for short overall survival (OS) and progression-free survival (PFS) in NSCLC patients. Next, we validated that circMET overexpression can enhance NSCLC cell proliferation, metastasis, and immune evasion in vitro. Mechanistically, our study uncovers that circMET acts as a miR-145-5p sponge to upregulate CXCL3 expression. Collectively, circMET regulates the miR-145-5p/CXCL3 axis and serves as a novel, promising diagnostic and prognostic biomarker in patients with NSCLC.
['Pei|Xu|X|', 'Chen|Shi-Wei|SW|', 'Long|Xiang|X|', 'Zhu|Shu-Qiang|SQ|', 'Qiu|Bai-Quan|BQ|', 'Lin|Kun|K|', 'Lu|Feng|F|', 'Xu|Jian-Jun|JJ|', 'Zhang|Peng-Fei|PF|', 'Wu|Yong-Bing|YB|']
D014408:Biomarkers, Tumor D002289:Carcinoma, Non-Small-Cell Lung D049109:Cell Proliferation D019743:Chemokines, CXC D005260:Female D006801:Humans D057131:Immune Evasion D008175:Lung Neoplasms D008297:Male D035683:MicroRNAs D008875:Middle Aged D019859:Proto-Oncogene Proteins c-met D000079962:RNA, Circular
circMET NSCLC proliferation miR-145-5p CXCL3