Biological properties of Beet soil-borne mosaic virus and Beet necrotic yellow vein virus cDNA clones produced by isothermal in vitro recombination: Insights for reassortant appearance.
Two members of the Benyviridae family and genus Benyvirus, Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV), possess identical genome organization, host range and high sequence similarity; they infect Beta vulgaris with variable symptom expression. In the US, mixed infections are described with limited information about viral interactions. Vectors suitable for agroinoculation of all genome components of both viruses were constructed by isothermal in vitro recombination. All 35S promoter-driven cDNA clones allowed production of recombinant viruses competent for Nicotiana benthamiana and Beta macrocarpa systemic infection and Polymyxa betae transmission and were compared to available BNYVV B-type clone. BNYVV and BSBMV RNA1 + 2 reassortants were viable and spread long-distance in N. benthamiana with symptoms dependent on the BNYVV type. Small genomic RNAs were exchangeable and systemically infected B. macrocarpa. These infectious clones represent a powerful tool for the identification of specific molecular host-pathogen determinants.
['Laufer|Marlene|M|', 'Mohammad|Hamza|H|', 'Maiss|Edgar|E|', 'Richert-Pöggeler|Katja|K|', "Dall'Ara|Mattia|M|", 'Ratti|Claudio|C|', 'Gilmer|David|D|', 'Liebe|Sebastian|S|', 'Varrelmann|Mark|M|']
D027461:Beta vulgaris D003001:Cloning, Molecular D018076:DNA, Complementary D015967:Gene Expression Regulation, Viral D009029:Mosaic Viruses D010935:Plant Diseases D018515:Plant Leaves D010942:Plant Viruses D012328:RNA Viruses D012367:RNA, Viral D016865:Reassortant Viruses
Beet soil-borne mosaic virus Beet necrotic yellow vein virus Reassortment Benyviridae Polymyxa betae Gibson assembly Infectious cDNA full-length clones