Aluminum Trichloride Inhibits the Rat Osteoblasts Mineralization In Vitro.
Aluminum (Al) is an accumulative toxic metal. Excessive Al accumulation inhibits osteoblasts mineralization and induces osteoporosis. However, the inhibition mechanism of Al on the mineralization is not fully understood. Thus, in this study, the rat osteoblasts were cultured and exposed to 0 mmol L-1 (control group, CG) and 0.52 mmol L-1 aluminum trichloride (AlCl3, treatment group, TG) for 7, 14, and 21 days, respectively. We found that mineralized matrix nodules, the activity of bone alkaline phosphatase, the concentration of extracellular calcium, the mRNA expression of type-I collagen, the mRNA and protein expressions of osteopontin, osteocalcin, and bone sialoprotein were all decreased, while the concentration of extracellular phosphorus was increased in TG compared with CG with time prolonged. Taken together, these results indicated that AlCl3 inhibited osteoblasts mineralization in vitro.
['Song|Miao|M|', 'Huo|Hui|H|', 'Cao|Zheng|Z|', 'Han|Yanfei|Y|', 'Gao|Li|L|']
D000077410:Aluminum Chloride D017607:Aluminum Compounds D000818:Animals D002113:Calcification, Physiologic D002478:Cells, Cultured D002712:Chlorides D005260:Female D008297:Male D010006:Osteoblasts D051381:Rats D017207:Rats, Sprague-Dawley D013997:Time Factors
Aluminum trichloride Rat Osteoblasts Mineralization