The kinase CIPK11 functions as a positive regulator in cadmium stress response in Arabidopsis.
Cadmium (Cd) pollution in agricultural soil has always been a knotty problem, which made it necessary to find the mechanism related to Cd transport in plant. In this study, we found a novel character of the CIPK11 modulating the transport of Cd in Arabidopsis thaliana. Over-expression of CIPK11 (CIPK11OE#1-7, CIPK11OE#8-5) resulted in the increased tolerance to Cd stress, which embodied in higher fresh weight, lower Cd enrichment and reactive oxygen species (ROS) than the wild-type (WT) plants. qRT-PCR results showed a collective down-regulation of the expression of IRT1 and transcription factor genes FIT, bHLH039 in the CIPK11-overexpression plants after Cd stress. Overexpression of CIPK11 significantly increased the expression of ABA marker genes in Arabidopsis after Cd stress. With different concentrations of ABA treatment, the root length differences caused by Cd stress could be recovered. However the transcription levels of FIT and bHLH039 decreased in WT and cipk11 mutant when treated with ABA which indicated that ABA can inhibit the transcription of IRT1 by repressing FIT and bHLH039 expression. Taken together, our results demonstrated that the kinase CIPK11 responses to Cd stress by ABA signaling pathway.
['Gu|Shaobo|S|', 'Wang|Xin|X|', 'Bai|Jiuyuan|J|', 'Wei|Tao|T|', 'Sun|Manli|M|', 'Zhu|Lin|L|', 'Wang|Maolin|M|', 'Zhao|Yun|Y|', 'Wei|Wei|W|']
D000040:Abscisic Acid D017360:Arabidopsis D029681:Arabidopsis Proteins D002104:Cadmium D018506:Gene Expression Regulation, Plant D009154:Mutation D018517:Plant Roots D030821:Plants, Genetically Modified D017346:Protein Serine-Threonine Kinases D017382:Reactive Oxygen Species D013312:Stress, Physiological
Kinase CIPK11 Cadmium Arabidopsis thaliana Abscisic acid