A bovine herpesvirus 1 pUL51 deletion mutant shows impaired viral growth in vitro and reduced virulence in rabbits.
Bovine herpesvirus 1 (BoHV-1) UL51 protein (pUL51) is a tegument protein of BoHV-1 whose function is currently unknown. Here, we aimed to illustrate the specific role of pUL51 in virion morphogenesis and its importance in BoHV-1 virulence. To do so, we constructed a BoHV-1 bacterial artificial chromosome (BAC). We used recombinant BAC and transgenic techniques to delete a major part of the UL51 open reading frame. Deletion of pUL51 resulted in severe viral growth defects, as evidenced by lower single and multi-step growth kinetics, reduced plaque size, and the accumulation of non-enveloped capsids in the cytoplasm of infected cells. Using tagged BoHV-1 recombinant viruses, it was determined that the pUL51 protein completely co-localized with the cis-Golgi marker protein GM-130. Taken altogether, pUL51 was demonstrated to play a critical role in BoHV-1 growth and it is involved in viral maturation and egress. Moreover, an in vivo analysis showed that the pUL51 mutant exhibited reduced virulence in rabbits, with no clinical signs, no nasal shedding of the virus, and no detectable serum neutralizing antibodies. Therefore, we conclude that the BoHV-1 pUL51 is indispensable for efficient viral growth in vitro and is essential for virulence in vivo.
['Raza|Sohail|S|', 'Deng|Mingliang|M|', 'Shahin|Farzana|F|', 'Yang|Kui|K|', 'Hu|Changmin|C|', 'Chen|Yingyu|Y|', 'Chen|Huanchun|H|', 'Guo|Aizhen|A|']
D000818:Animals D002417:Cattle D007242:Herpesvirus 1, Bovine D011817:Rabbits D015678:Viral Structural Proteins D014774:Virulence D014779:Virus Replication
bovine herpesvirus 1 pUL51 tegument protein morphogenesis bacterial artificial chromosome Immune response Immunity Immunology and Microbiology Section