Structures, Mechanisms, and Functions of His-Me Finger Nucleases.
His-Me finger (also called HNH or ββα-me) nucleases, are a large superfamily of nucleases that share limited sequence homology, but all members carry a highly similar catalytic motif exhibiting a ββα topology. This review represents a structural comparison of His-Me finger nucleases, summarizing their substrate-binding and recognition strategies, mechanisms of enzymatic hydrolysis, cellular functions, and the various means of activity regulation. His-Me finger nucleases usually function as monomers, making a single nick in nucleic acids to degrade foreign or host genomes, or as homodimers that introduce double-stranded DNA breaks for DNA restriction, integration, recombination, and repair. Various cellular neutralizing machineries have evolved to regulate the activity of His-Me finger nucleases, thereby maintaining genome integrity and cellular functionality.
['Wu|Chyuan-Chuan|CC|', 'Lin|Jason L J|JLJ|', 'Yuan|Hanna S|HS|']
D000818:Animals D055162:Biocatalysis D004720:Endonucleases D006801:Humans D008958:Models, Molecular D011487:Protein Conformation
DNA cleavage DNA hydrolysis DNase HNH family