Fabrication of Chitosan/Polypyrrole-coated poly(L-lactic acid)/Polycaprolactone aligned fibre films for enhancement of neural cell compatibility and neurite growth.
OBJECTIVE
Chitosan (CS) and polycaprolactone (PCL) were added into a nerve scaffold of poly(L-lactide acid) (PLLA)/polypyrrole (PPy)-based fibre films to solve the unmatch with the nerve strength and the aseptic inflammation from PLLA.


METHODS
Poly (L-lactide acid)-polycaprolactone (PLLA/PCL) fibre films coated with chitosan (CS) and polypyrrole (PPy) were prepared by electrospinning of aligned PLLA/PCL fibres, electrochemical deposition of PPy nanoparticles and in situ doping of CS in PPy. PC12 cells were electrically stimulated with 100 mV for 2 hours every day via CS/PPy-PLLA/PCL fibre film to promote the neurite growth.


RESULTS
The surface conductivity and tensile strength of CS/PPy-PLLA/PCL fibre films were 1.03 s/m and 13 MPa, respectively. CS content in fibre films was about 7.5 mg/cm2 , improving the pH value (reached to 5.1) of immersion solution of the fibre film at 16 days. Compared with PPy-PLLA/PCL fibre film, more and longer axons were grown out from PC12 cells cultured on CS/PPy-PLLA/PCL fibre film, indicating the positive effect of CS in fibre film on axon growth. The cell differentiation rate and neurite length on CS/PPy-PLLA/PCL fibre film reached to 38% and 75 μm, respectively. These results suggest the promotion of electrical stimulation on neurite growth and alignment.


CONCLUSIONS
A synergistic mechanism about the promotion of CS, electrical stimulation and aligned fibres on PC12 cells differentiation, axon outgrowth was proposed. These results indicated the potential application of CS/PPy-PLLA/PCL fibre film in the field of the nerve repair and regeneration.
['Xu|Yaxuan|Y|', 'Huang|Zhongbing|Z|https://orcid.org/0000-0001-9210-1842', 'Pu|Ximing|X|', 'Yin|Guangfu|G|', 'Zhang|Jiankai|J|']
D000818:Animals D002454:Cell Differentiation D048271:Chitosan D004553:Electric Conductivity D007739:L Cells D008422:Materials Testing D051379:Mice D008855:Microscopy, Electron, Scanning D008959:Models, Neurological D057139:Nanofibers D009416:Nerve Regeneration D016501:Neurites D055495:Neurogenesis D009474:Neurons D016716:PC12 Cells D011091:Polyesters D011108:Polymers D011758:Pyrroles D051381:Rats
chitosan neurite growth electrical stimulation cell differentiation conductive aligned fibre-film