Scaffold/matrix attachment regions from CHO cell chromosome enhanced the stable transfection efficiency and the expression of transgene in CHO cells.
Two scaffold/matrix attachment regions (S/MARs) from Chinese hamster ovary (CHO) cell chromosomes were identified computationally and cloned as the domain boundaries regions. As partition chromosomes in independently regulated units to increase the exogenous gene expression, these two S/MARs (MAR1 and MAR6) flanked expression vectors were respectively transfected into CHO cells. The results showed that MAR1 and MAR6 were much more potent to increase the stable transfection efficiency than the chicken lysozyme gene 5' S/MARs, which had been experimentally identified to increasing the expression of heterologous gene. Moreover, MAR6 is more active in mediating high and consistent gene expression in CHO cells than the chicken lysozyme gene 5' S/MARs.
['Chang|Ming|M|', 'Liu|Ruijie|R|', 'Jin|Qingzhe|Q|', 'Liu|Yuanfa|Y|', 'Wang|Xingguo|X|']
D000818:Animals D016466:CHO Cells D003198:Computer Simulation D006224:Cricetinae D003412:Cricetulus D018628:Gene Dosage D042822:Genomic Instability D045170:Matrix Attachment Regions D011994:Recombinant Proteins D014162:Transfection D019076:Transgenes
scaffold/matrix attachment region Chinese hamster ovary cells high expression integration efficiency