Production and characterization of monoclonal antibodies against highly immunogenic Opisthorchis viverrini proteins and development of coproantigen detection.
Opisthorchis viverrini and other foodborne trematode infections are major health concerns in the Greater Mekong Subregion. Currently, the gold-standard diagnostic method for opisthorchiasis is conventional stool examination for the presence of parasite eggs. This method lacks sensitivity and needs an experienced technician. We therefore produced monoclonal antibodies to highly immunogenic O. viverrini proteins aiming at detecting specific antigens in the feces. In this study, BALB/C mice were immunized using semi-purified somatic antigens and spleen cells were fused with a Sp2/0 myeloma cell line. Four hybridomas (1A2, 1E12, 2C7 and 8D6) were selected and cloned due to their strong reaction against O. viverrini somatic protein, resulting in three IgM clones and one IgG2 clone. Immunohistochemistry showed that 1A2, 1E12, 2C7 and 8D6 stained the parenchyma cells, gut, tegument and muscles, respectively. Western-blot analysis revealed that only antibody 1A2 could detect coproantigen (approx. 73 kDa protein) in feces of hamsters infected with O. viverrini. The 1A2 monoclonal antibody may be of value in the diagnosis of opisthorchiasis by coproantigen detection.
['Arimatsu|Yuji|Y|', 'Teimoori|Salma|S|', 'Surapaitoon|Arpa|A|', 'Sripa|Banchob|B|']
D000818:Animals D000909:Antibodies, Helminth D000911:Antibodies, Monoclonal D000917:Antibody Formation D000947:Antigens, Helminth D045744:Cell Line, Tumor D004797:Enzyme-Linked Immunosorbent Assay D015801:Helminth Proteins D006801:Humans D006825:Hybridomas D007118:Immunoassay D007159:Immunologic Tests D009889:Opisthorchiasis D009891:Opisthorchis
Monoclonal antibody Opisthorchis viverrini Coproantigen Immunodiagnosis Immunogenic fractions