Endoplasmic reticulum stress in the livers of BDNF heterozygous knockout mice.
Context: Involvement of endoplasmic reticulum (ER) stress and brain-derived neurotrophic factor (BDNF) in hepatic lipid metabolism has been reported previously. Objective: The effects of chronic BDNF deficiency on ER stress response in the livers were examined in this study. Methods: BDNF(+/-) mice, characterised by BDNF deficiency, and their wild-type (WT) littermates were used. The ER stress was induced by tunicamycin (Tm) (0.5 mg/kg, intraperitoneal). Animals were divided into four groups; WT, WT + Tm, BDNF(+/-), and BDNF(+/-)+Tm. Results: At the basal conditions, BDNF deficiency did not affect hepatic cell death or lipid accumulation. However, during ER stress, BDNF(+/-)+Tm group showed increased apoptosis, GADD153 immunostaining, sterol regulatory element-binding protein-1c (SREBP-1c) level, and steatosis compared to the WT + Tm group. Conclusion: Endogenous BDNF might be protective against apoptosis through GADD153 suppression and steatosis via SREBP-1c suppression during ER stress. This effect of BDNF might be clinically important for type 2 diabetes and obesity, which are related with both ER stress and BDNF deficiency.
['Cırrık|Selma|S|http://orcid.org/0000-0001-8474-0185', 'Hacioglu|Gulay|G|', 'Abidin|İsmail|İ|', 'Aydın-Abidin|Selcen|S|', 'Noyan|Tevfik|T|']
D000818:Animals D019208:Brain-Derived Neurotrophic Factor D053454:Caspase 12 D000091342:Endoplasmic Reticulum Chaperone BiP D059865:Endoplasmic Reticulum Stress D006360:Heat-Shock Proteins D006579:Heterozygote D008099:Liver D051379:Mice D018345:Mice, Knockout D047493:PPAR alpha D051742:Transcription Factor CHOP
endoplasmic reticulum stress BDNF heterozygous knockout mice apoptosis SREBP-1c steatosis