Voltammetric sensor based on carbon paste electrode modified with molecular imprinted polymer for determination of sulfadiazine in milk and human serum.
A new sensitive voltammetric sensor for determination of sulfadiazine is described. The developed sensor is based on carbon paste electrode modified with sulfadiazine imprinted polymer (MIP) as a recognition element. For comparison, a non-imprinted polymer (NIP) modified carbon paste electrode was prepared. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods were performed to study the binding event and electrochemical behavior of sulfadiazine at the modified carbon paste electrodes. The determination of sulfadiazine after its extraction onto the electrode surface was carried out by DPV at 0.92 V vs. Ag/AgCl owing to oxidation of sulfadiazine. Under the optimized operational conditions, the peak current obtained at the MIP modified carbon paste electrode was proportional to the sulfadiazine concentration within the range of 2.0×10(-7)-1.0×10(-4) mol L(-1) with a detection limit and sensitivity of 1.4×10(-7) mol L(-1) and 4.2×10(5) μA L mol(-1), respectively. The reproducibility of the developed sensor in terms of relative standard deviation was 2.6%. The sensor was successfully applied for determination of sulfadiazine in spiked cow milk and human serum samples with recovery values in the range of 96.7-100.9%.
['Sadeghi|Susan|S|', 'Motaharian|Ali|A|']
D000818:Animals D000890:Anti-Infective Agents D002244:Carbon D002417:Cattle D055664:Electrochemical Techniques D004566:Electrodes D006801:Humans D006863:Hydrogen-Ion Concentration D008892:Milk D054802:Molecular Imprinting D010084:Oxidation-Reduction D011108:Polymers D013411:Sulfadiazine
Voltammetric sensor Molecular imprinted polymer Sulfadiazine Modified carbon paste electrode