An Alternative Cuprizone-Induced Demyelination and Remyelination Mouse Model.
The cuprizone model is a well-established and investigated paradigm to study demyelination and remyelination in rodents. Cuprizone is usually administrated by mixing in the powdered or pelleted rodent chow. However, since cuprizone is sensitive to the environment and the consumption of it varies between different animals, the major issue is the discrepancy in demyelination of the animals. This study reports the development of the cuprizone model by gavage administrations in mice. Following testing a series of doses of cuprizone, 400 mg/kg/day was found to be the best dosage to induce dramatic and consistent demyelination after 5 weeks of administration; while remyelination quickly occurred after 9 days of cuprizone withdrawal. The advantage of this alternative model is that the consumption of cuprizone could be well controlled, and the mice were exposed to the same dose of cuprizone. Thus, the variation in demyelination was minimized. This alternative cuprizone dosing regime minimizes the interanimal variability on demyelination and hence provides a consistent model for pharmacological evaluations, in addition to reducing the number of animals used in the experiments.
['Zhen|Wei|W|', 'Liu|Ailian|A|', 'Lu|Jiaqi|J|', 'Zhang|Wandong|W|', 'Tattersall|David|D|', 'Wang|Jianfei|J|']
D000284:Administration, Oral D000818:Animals D001835:Body Weight D003471:Cuprizone D003711:Demyelinating Diseases D004195:Disease Models, Animal D004305:Dose-Response Relationship, Drug D008297:Male D008810:Mice, Inbred C57BL D000074586:Remyelination D012249:Rhombencephalon D016019:Survival Analysis
demyelination remyelination cuprizone animal model oral administration