Identification of novel microRNA genes in freshwater and marine ecotypes of the three-spined stickleback (Gasterosteus aculeatus).
The three-spined stickleback (Gasterosteus aculeatus L.) is an important model organism for studying the molecular mechanisms of speciation and adaptation to salinity. Despite increased interest to microRNA discovery and recent publication on microRNA prediction in the three-spined stickleback using bioinformatics approaches, there is still a lack of experimental support for these data. In this paper, high-throughput sequencing technology was applied to identify microRNA genes in gills of the three-spined stickleback. In total, 595 miRNA genes were discovered; half of them were predicted in previous computational studies and were confirmed here as microRNAs expressed in gill tissue. Moreover, 298 novel microRNA genes were identified. The presence of miRNA genes in selected 'divergence islands' was analysed and 10 miRNA genes were identified as not randomly located in 'divergence islands'. Regulatory regions of miRNA genes were found enriched with selective SNPs that may play a role in freshwater adaptation.
['Rastorguev|S M|SM|', 'Nedoluzhko|A V|AV|', 'Sharko|F S|FS|', 'Boulygina|E S|ES|', 'Sokolov|A S|AS|', 'Gruzdeva|N M|NM|', 'Skryabin|K G|KG|', 'Prokhortchouk|E B|EB|']
D000818:Animals D060146:Ecotype D005618:Fresh Water D005880:Gills D059014:High-Throughput Nucleotide Sequencing D035683:MicroRNAs D012623:Seawater D023701:Smegmamorpha
gasterosteus aculeatus speciation adaptation miRNA gene structure and function