Tripterygium glycosides impairs the proliferation of granulosa cells and decreases the reproductive outcomes in female rats.
This study was carried out to investigate the impact of tripterygium glycosides (TGs) on ovarian function of female rats in vitro and in vivo. In vitro studies showed that TG induced cells decrease at G1 phase and inhibited cell proliferation in rat granulosa cells. In vivo, female rats were intragastrically administered with TG at the dose of 60 mg/kg/day for consecutive 50 days. TG caused a prolonged estrous cycle, and a significant reduction in ovarian index, serum E2 level, and numbers of secondary and antral follicles (p < 0.05) in these rats. A significant reduction of viable embryos was demonstrated in TG-treated female rats after mating (p < 0.01). Further, we observed observed the reduced expression level of TGF-β1 after TG treatment in vitro and in vivo. Moreover, the expression of Smad2 and AKT was also decreased after TG treatment. These results suggest that TG can impair ovarian function through Smads-mediated TGF-β1 signal pathway.
['Su|Jing|J|', 'Cheng|Jie|J|', 'Sun|Hai-Xiang|HX|', 'Diao|Zhen-Yu|ZY|', 'Zhen|Xin|X|', 'Yang|Jun|J|', 'Ding|Li-Jun|LJ|', 'Hu|Ya-Li|YL|']
D000818:Animals D017209:Apoptosis D002453:Cell Cycle D049109:Cell Proliferation D002478:Cells, Cultured D056742:Cyclin D2 D004224:Diterpenes D004305:Dose-Response Relationship, Drug D004852:Epoxy Compounds D005260:Female D006027:Glycosides D006107:Granulosa Cells D010616:Phenanthrenes D051381:Rats D017207:Rats, Sprague-Dawley D012098:Reproduction D051899:Smad2 Protein D053773:Transforming Growth Factor beta1 D029757:Tripterygium
tripterygium glycosides granulosa cells TGF-β1 ovary triptolide