Evidence that osteogenic and neurogenic differentiation capability of epidural adipose tissue-derived stem cells was more pronounced than in subcutaneous cells
Background/aim
The  management  of  dura-related  complications,  such  as  the  repairment  of  dural  tears  and  reconstruction  of  large  dural defects, remain the most challenging subjects of neurosurgery. Numerous surgical techniques and synthetic or autologous adjuvant materials  have  emerged  as  an  adjunct  to  primary  dural  closure,  which  may  result  in  further  complications  or  side  effects.  Therefore,  the  subcutaneous  autologous  free  adipose  tissue  graft  has  been  recommended  for  the  protection  of  the  central  nervous  system  and  repairment of the meninges. In addition, human adipose tissue is also a source of multipotent stem cells. However, epidural adipose tissue seems more promising than subcutaneous because of the close location and intercellular communication with the spinal cord. Herein, it was aimed to define differentiation capability of both subcutaneous and epidural adipose tissue-derived stem cells (ASCs).


Materials and methods
Human subcutaneous and epidural adipose tissue specimens were harvested from the primary incisional site and the lumbar epidural space during lumbar spinal surgery, and ASCs were isolated.


Results
The results indicated that both types of ASCs expressed the cell surface markers, which are commonly expressed stem cells; however,  epidural  ASCs  showed  lower  expression  of  CD90  than  the  subcutaneous  ASCs.  Moreover,  it  was  demonstrated  that  the  osteogenic  and  neurogenic  differentiation  capability  of  epidural  adipose  tissue-derived  ASCs  was  more  pronounced  than  that  of  the  subcutaneous ASCs.


Conclusion
Consequently,  the  impact  of  characterization  of  epidural  ASCs  will  allow  for  a  new  understanding  for  dural  as  well  as  central nervous system healing and recovery after an injury.
['Solmaz|Bilgehan|B|0000-0003-2015-9484', 'Şahin|Ali|A|0000-0001-5594-1551', 'Keleştemur|Taha|T|0000-0003-3616-1204', 'Kiliç|Ertuğrul|E|0000-0001-6494-8923', 'Kaptanoğlu|Erkan|E|0000-0002-9945-8817']
D000273:Adipose Tissue D002454:Cell Differentiation D002478:Cells, Cultured D004824:Epidural Space D006801:Humans D055495:Neurogenesis D010012:Osteogenesis D013234:Stem Cells D050151:Subcutaneous Fat
differentiation stem cell Epidural adipose tissue CD90 CD105