Fabrication of novel poly(lactic acid/caprolactone) bilayer membrane for GBR application.
OBJECTIVE
Guided bone regeneration (GBR) often involves the use of membranes as barriers for soft tissues. Commercially available membranes, however, do not possess an adequately low degradation rate, resulting in limited barrier function. The purpose of this study was to develop and assess the physicochemical and biological characteristics of a novel poly(l-lactic acid/caprolactone) (PLCL) bilayer membrane and determine its usefulness for GBR application.


METHODS
The experimental bilayer membrane was prepared via a two-step freezing and lyophilization process with a PLCL solution. Next, the PLCL membrane was investigated regarding tensile strength, surface roughness, in vitro degradation and clinical operability. In addition, cell proliferation and differentiation were investigated on each layer of the experimental membrane. For all experiments, a commercially available poly(lactic-co-glycolic) acid membrane was used as a control.


RESULTS
In vitro analysis of the PLCL bilayer membrane revealed suitable mechanical strength combined with high breaking strain, which contributed to membrane operability. In addition, the PLCL bilayer membrane had enhanced stability compared to the commercial control due to its slower degradation, and was capable of supporting cell growth and osteogenic differentiation.


SIGNIFICANCE
The current study confirmed that the PLCL membrane possessed a high biocompatibility and slow degradation rate that contributes to prolonged barrier function and bone regeneration. Altogether, it was considered that the PLCL bilayer membrane developed in this study was applicable for GBR treatment.
['Abe|Gabriela L|GL|', 'Sasaki|Jun-Ichi|JI|', 'Katata|Chihiro|C|', 'Kohno|Tomoki|T|', 'Tsuboi|Ririko|R|', 'Kitagawa|Haruaki|H|', 'Imazato|Satoshi|S|']
D001672:Biocompatible Materials D001861:Bone Regeneration D002208:Caproates D007783:Lactones D010012:Osteogenesis D011091:Polyesters D000077182:Polylactic Acid-Polyglycolic Acid Copolymer D023822:Tissue Engineering
Caprolactone GBR Bone regeneration Biodegradable membrane Copolymer