In utero exposure of rats to high-fat diets perturbs gene expression profiles and cancer susceptibility of prepubertal mammary glands.
Human studies suggest that high-fat diets (HFDs) increase the risk of breast cancer. The 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary carcinogenesis rat model is commonly used to evaluate the effects of lifestyle factors such as HFD on mammary tumor risk. Past studies focused primarily on the effects of continuous maternal exposure on the risk of offspring at the end of puberty (PND50). We assessed the effects of prenatal HFD exposure on cancer susceptibility in prepubertal mammary glands and identified key gene networks associated with such disruption. During pregnancy, dams were fed AIN-93G-based diets with isocaloric high olive oil, butterfat or safflower oil. The control group received AIN-93G. Female offspring were treated with DMBA on PND21. However, a significant increase in tumor volume and a trend of shortened tumor latency were observed in rats with HFD exposure against the controls (P=.048 and P=.067, respectively). Large-volume tumors harbored carcinoma in situ. Transcriptome profiling identified 43 differentially expressed genes in the mammary glands of the HFBUTTER group as compared with control. Rapid hormone signaling was the most dysregulated pathway. The diet also induced aberrant expression of Dnmt3a, Mbd1 and Mbd3, consistent with potential epigenetic disruption. Collectively, these findings provide the first evidence supporting susceptibility of prepubertal mammary glands to DMBA-induced tumorigenesis that can be modulated by dietary fat that involves aberrant gene expression and likely epigenetic dysregulation.
['Govindarajah|Vinothini|V|', 'Leung|Yuet-Kin|YK|', 'Ying|Jun|J|', 'Gear|Robin|R|', 'Bornschein|Robert L|RL|', 'Medvedovic|Mario|M|', 'Ho|Shuk-Mei|SM|']
D015127:9,10-Dimethyl-1,2-benzanthracene D000818:Animals D002273:Carcinogens D059305:Diet, High-Fat D005260:Female D020869:Gene Expression Profiling D020022:Genetic Predisposition to Disease D008325:Mammary Neoplasms, Experimental D011247:Pregnancy D051381:Rats D017207:Rats, Sprague-Dawley D012741:Sexual Maturation
Breast cancer Epigenetics DNA methyl transferases DNA methylation Developmental origin of health and disease Methylated DNA binding domain