MiR-106a Associated with Diabetic Peripheral Neuropathy Through the Regulation of 12/15-LOX-meidiated Oxidative/Nitrative Stress.
BACKGROUND
Diabetic peripheral neuropathy (DPN) is a common complication of diabetes with a complex pathogenesis. Study has reported that oxidative stress and nitrative stress are all involved in the DPN. However, the mechanism between them is still unclear. In the present study, we investigated whether miR-106a regulated 12/15-Lipoxygenase (12/15-LOX) of oxidative/nitrative stress (OS/NS) in DPN.


METHODS
Dorsal root ganglion (DRG) was isolated from 10 healthy mice and 10 DPN mice. DPN mouse model was established by the injection of streptozotocin (STZ), and high glucose was used for inducing the in vitro model of DPN.


RESULTS
In DPN mice, the levels of fasting blood-glucose (FBG) level, Methane Dicarboxylic Aldehyde (MDA) and the Inducible Nitric Oxide Synthase (iNOS) were increased, while the levels of motor nerve conduction velocity (MNCV), sensory nerve conduction velocity (SNCV) and superoxide dismutase (SOD) were decreased. MiR-106a level in DRG cells of DPN was decreased. The 12/15-LOX expression and cell apoptosis were increased. DRG cells subjected to high glucose resulted in the same effects as above. MiR-106a was observed to target 12/15-LOX and regulated its expression. MiR-106a overexpression reversed the effect that was induced by high glucose, however, overexpression of 12/15-LOX reversed the effect that was induced by miR-106a overexpression.


CONCLUSION
MiR-106 may be associated with 12/15-LOX mediated OS/NS in DPN and may be considered as a potential therapeutic target.
['Wu|Yuanbo|Y|', 'Xu|Dandan|D|', 'Zhu|Xiang|X|', 'Yang|Guangwei|G|', 'Ren|Mingshan|M|']
D000818:Animals D000903:Antibiotics, Antineoplastic D001092:Arachidonate 12-Lipoxygenase D001093:Arachidonate 15-Lipoxygenase D001786:Blood Glucose D003929:Diabetic Neuropathies D004195:Disease Models, Animal D005215:Fasting D005727:Ganglia, Spinal D005947:Glucose D051379:Mice D008810:Mice, Inbred C57BL D035683:MicroRNAs D009431:Neural Conduction D009474:Neurons D052247:Nitric Oxide Synthase Type II D018384:Oxidative Stress D011897:Random Allocation D013311:Streptozocin D013482:Superoxide Dismutase D045162:Thiazolidinediones D015854:Up-Regulation
Diabetic peripheral neuropathy nitrative stress oxidative stress 12/15-LOX diabetic mellitus micro-106a