EGCG and enzymatic cross-linking combined treatments for improving elastin stability and reducing calcification in bioprosthetic heart valves.
The failures of glutaraldehyde (GLUT) cross-linked bioprosthetic heart valves (BHVs) are mainly due to degeneration and calcification. In this study, we developed a new preparation strategy for BHVs named as "HPA/EDC/EGCG" that utilized 3,4-hydroxyphenylpropionic acid (HPA)-conjugated pericardium, epigallocatechin gallate (EGCG), and horseradish peroxidase (HRP)/hydrogen peroxide (H2 O2 ) enzymatic cross-linking. HPA-pericardium conjugation was done by carbodiimide coupling reaction using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Then HPA-conjugated pericardium was cross-linked by HRP/H2 O2 enzyme-catalyzed oxidation. The feeding ratios of HPA and EGCG were optimized. The consumption of amino groups, collagenase and elastase degradation in vitro, biomechanics, extracellular matrix stability, and calcification of HPA-/EDC-/EGCG-treated pericardiums were characterized. We demonstrated that HPA-/EDC-/EGCG-treated pericardiums had better elastin stabilization and less calcification. EGCG and enzymatic cross-linking treated pericardiums showed improved mechanical properties. This new EGCG and enzymatic cross-linking strategy would be a promising method to make BHVs with better elastin stability and anti-calcification property. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1551-1559, 2019.
['Lei|Yang|Y|0000-0003-1461-8111', 'Yang|Li|L|', 'Guo|Gaoyang|G|', 'Wang|Yunbing|Y|']
D001566:Benzocaine D001696:Biomechanical Phenomena D001705:Bioprosthesis D001777:Blood Coagulation D002113:Calcification, Physiologic D002392:Catechin D002701:Chloramphenicol D003432:Cross-Linking Reagents D003895:Desmosine D004338:Drug Combinations D004549:Elastin D005022:Ethyldimethylaminopropyl Carbodiimide D005109:Extracellular Matrix D005976:Glutaral D006350:Heart Valve Prosthesis D006351:Heart Valves D006735:Horseradish Peroxidase D006801:Humans D006861:Hydrogen Peroxide D009583:Nitrofurazone D010496:Pericardium
elastin stabilization calcification bioprosthetic heart valves 3,4-hydroxyphenylpropionic acid horseradish peroxidase and hydrogen peroxide