Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth.
Elucidating the local function of proteins is essential for understanding not only the individual proteins but also the organization of the cell or even tissue as a whole. However, until now, few attempts have been made to understand local proteins function in cells because of a lack of acute inactivation technique of local proteins with high versatility. Here we describe the application of the chromophore-assisted light inactivation (CALI) method to elucidate the role of the semaphorin signaling component CRMP located within the growth cone area in axon growth and growth cone turning.
['Goshima|Yoshio|Y|', 'Takei|Kohtaro|K|']
D000818:Animals D001369:Axons D019556:COS Cells D002522:Chlorocebus aethiops D005727:Ganglia, Spinal D020439:Growth Cones D057809:HEK293 Cells D006801:Humans D008027:Light D009474:Neurons D039961:Semaphorins D015398:Signal Transduction
Semaphorin CRMP Axon Chromophore-assisted light inactivation (CALI) Growth cone Axon guidance Cell signaling Neurite extension