Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating TAZ Activity.
Cell fate specification by lateral inhibition typically involves contact signaling through the Delta-Notch signaling pathway. However, whether this is the only signaling mode mediating lateral inhibition remains unclear. Here we show that in zebrafish oogenesis, a group of cells within the granulosa cell layer at the oocyte animal pole acquire elevated levels of the transcriptional coactivator TAZ in their nuclei. One of these cells, the future micropyle precursor cell (MPC), accumulates increasingly high levels of nuclear TAZ and grows faster than its surrounding cells, mechanically compressing those cells, which ultimately lose TAZ from their nuclei. Strikingly, relieving neighbor-cell compression by MPC ablation or aspiration restores nuclear TAZ accumulation in neighboring cells, eventually leading to MPC re-specification from these cells. Conversely, MPC specification is defective in taz-/- follicles. These findings uncover a novel mode of lateral inhibition in cell fate specification based on mechanical signals controlling TAZ activity.
['Xia|Peng|P|', 'Gütl|Daniel|D|', 'Zheden|Vanessa|V|', 'Heisenberg|Carl-Philipp|CP|']
D048868:Adaptor Proteins, Signal Transducing D000818:Animals D002450:Cell Communication D002454:Cell Differentiation D019070:Cell Lineage D002467:Cell Nucleus D005260:Female D006107:Granulosa Cells D047908:Intracellular Signaling Peptides and Proteins D009865:Oocytes D009866:Oogenesis D017346:Protein Serine-Threonine Kinases D015398:Signal Transduction D014157:Transcription Factors D015533:Transcriptional Activation D000091364:Transcriptional Coactivator with PDZ-Binding Motif Proteins D015027:Zebrafish D029961:Zebrafish Proteins
lateral inhibition cell fate specification zebrafish oogenesis YAP/TAZ morphogenesis