Exogenous interleukin-2 can rescue in-vitro T cell activation and proliferation in patients with a novel capping protein regulator and myosin 1 linker 2 mutation.
Capping protein regulator and myosin 1 linker 2 (CARMIL2) deficiency is characterized by impaired T cell activation, which is attributed to defective CD28-mediated co-signaling. Herein, we aimed to analyze the effect of exogenous interleukin (IL)-2 on in-vitro T cell activation and proliferation in a family with CARMIL2 deficiency. This study included four children (one male and three females; aged 2·5-10 years at presentation). The patients presented with inflammatory bowel disease and recurrent viral infections. Genetic analysis revealed a novel homozygous 25-base pairs deletion in CARMIL2. Immunoblotting demonstrated the absence of CARMIL2 protein in all four patients and confirmed the diagnosis of CARMIL2 deficiency. T cells were activated in-vitro with the addition of IL-2 in different concentrations. CD25 and interferon (IFN)-γ levels were measured after 48 h and 5 days of activation. CD25 surface expression on activated CD8+ and CD4+ T cells was significantly diminished in all patients compared to healthy controls. Additionally, CD8+ T cells from all patients demonstrated significantly reduced IFN-γ production. When cells derived from CARMIL2-deficient patients were treated with IL-2, CD25 and IFN-γ production increased in a dose-dependent manner. T cell proliferation, as measured by Cell Trace Violet, was impaired in one patient and it was also rescued with IL-2. In conclusion, we found that IL-2 rescued T cell activation and proliferation in CARMIL2-deficient patients. Thus, IL-2 should be further studied as a potential therapeutic modality for these patients.
['Shamriz|O|O|', 'Simon|A J|AJ|', 'Lev|A|A|', 'Megged|O|O|', 'Ledder|O|O|', 'Picard|E|E|', 'Joseph|L|L|', 'Molho-Pessach|V|V|', 'Tal|Y|Y|', 'Millman|P|P|', 'Slae|M|M|', 'Somech|R|R|', 'Toker|O|O|', 'Berger|M|M|0000-0002-3469-0076']
D015496:CD4-Positive T-Lymphocytes D018414:CD8-Positive T-Lymphocytes D049109:Cell Proliferation D002648:Child D002675:Child, Preschool D005260:Female D006801:Humans D015212:Inflammatory Bowel Diseases D007376:Interleukin-2 D008213:Lymphocyte Activation D008297:Male D008840:Microfilament Proteins D009154:Mutation D014777:Virus Diseases
activation proliferation CARMIL2 T cell rescue primary immune deficiency