Differential distributions of trafficking and signaling proteins of the maize ER-Golgi apparatus.
The Endoplasmic Reticulum (ER)-Golgi apparatus of plants is the site of synthesis of non-cellulosic polysaccharides that then traffic to the cell wall. A two-step protocol of flotation centrifugation followed by free-flow electrophoresis (FFE) resolved ER and Golgi proteins into three profiles: an ER-rich fraction, two Golgi-rich fractions, and an intermediate fraction enriched in cellulose synthases. Nearly three dozen Rab-like proteins of eight different subgroups were distributed differentially in ER- vs. Golgi-rich fractions, whereas seven 14-3-3 proteins co-fractionated with cellulose synthases in the intermediate fraction. FFE offers a powerful means to classify resident and transient proteins in cell-free assays of cellular location.
['Okekeogbu|Ikenna O|IO|0000-0002-2215-2545', 'Aryal|Uma K|UK|0000-0003-4543-1536', 'Fernández-Niño|Susana M González|SMG|0000-0002-2135-4428', 'Penning|Bryan W|BW|0000-0002-0374-8693', 'Heazlewood|Joshua L|JL|0000-0002-2080-3826', 'McCann|Maureen C|MC|0000-0001-6956-4216', 'Carpita|Nicholas C|NC|0000-0003-0770-314X']
D004586:Electrophoresis D004721:Endoplasmic Reticulum D006056:Golgi Apparatus D018832:Molecular Chaperones D010940:Plant Proteins D021381:Protein Transport D015398:Signal Transduction D003313:Zea mays
Golgi 14-3-3 proteins Calcium-dependent proteins Cellulose synthase (CesA) Endoplasmic reticulum (ER) Maize (Zea mays) Small GTPase Rab proteins