Photosynthetic production of glycerol by a recombinant cyanobacterium.
Cyanobacteria are prokaryotic organisms capable of oxygenic photosynthesis. Glycerol is an important commodity chemical. Introduction of phosphoglycerol phosphatase 2 from Saccharomyces cerevisiae into the model cyanobacterium Synechocystis sp. PCC6803 resulted in a mutant strain that produced a considerable amount of glycerol from light, water and COPhotosynthetic production . Mild salt stress (200 mM NaCl) on the cells led to an increase of the extracellular glycerol concentration of more than 20%. Under these conditions the mutant accumulated glycerol to an extracellular concentration of 14.3 mM after 17 days of culturing.
['Savakis|Philipp|P|', 'Tan|Xiaoming|X|', 'Du|Wei|W|', 'Branco dos Santos|Filipe|F|', 'Lu|Xuefeng|X|', 'Hellingwerf|Klaas J|KJ|']
D000458:Cyanobacteria D004274:DNA, Recombinant D005990:Glycerol D060847:Metabolic Engineering D009154:Mutation D009997:Osmotic Pressure D010788:Photosynthesis D012965:Sodium Chloride
Glycerol Synechocystis Cell factory Metabolic engineering