Evaluation of poly (vinyl alcohol) based cryogel-zinc oxide nanocomposites for possible applications as wound dressing materials.
In this investigation cryogels composed of poly (vinyl alcohol) (PVA) were prepared by repeated freeze thaw method followed by in situ precipitation of zinc oxide nanoparticles within the cryogel networks. Fourier transformed infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) were used to characterize the nanocomposites. The morphologies of native PVA cryogels and PVA cryogel-ZnO nanocomposites were observed by scanning electron microscopy (SEM), transmission electron microscopy (TEM) techniques. The SEM analysis suggested that cryogels show a well-defined porous morphology whereas TEM micrographs revealed the presence of nearly spherical and well separated zinc oxide nanoparticles with diameter<100nm. XRD results showed all relevant Bragg's reflections for crystal structure of zinc oxide nanoparticles. Thermo gravimetric-differential thermal analysis (TG-DTA) was conducted to evaluate thermal stability of the nanocomposites. Mechanical properties of nanocomposites were determined in terms of tensile strength and percent elongation. Biocompatible nature was ascertained by anti-haemolytic activity, bovine serum albumin (blood protein) adsorption and in vitro cytotoxicity tests. The prepared nanocomposites were also investigated for swelling and deswelling behaviours. The results revealed that both the swelling and deswelling process depend on the chemical composition of the nanocomposites, number of freeze-thaw cycles, pH and temperature of the swelling medium. The developed biocompatible PVA cryogel-ZnO nanocomposites were also tested for antibacterial activities against both Gram-negative and Gram-positive bacteria.
['Chaturvedi|Archana|A|', 'Bajpai|Anil K|AK|', 'Bajpai|Jaya|J|', 'K Singh|Sunil|S|']
D000327:Adsorption D000818:Animals D000900:Anti-Bacterial Agents D001458:Bandages D002417:Cattle D059985:Cryogels D004912:Erythrocytes D006090:Gram-Negative Bacteria D006094:Gram-Positive Bacteria D006461:Hemolysis D006801:Humans D006863:Hydrogen-Ion Concentration D008855:Microscopy, Electron, Scanning D046529:Microscopy, Electron, Transmission D053761:Nanocomposites D011143:Polyvinyl Chloride D012710:Serum Albumin, Bovine D017550:Spectroscopy, Fourier Transform Infrared D013696:Temperature D013818:Thermogravimetry D014945:Wound Healing D015034:Zinc Oxide
Nanocomposite Cryogel Freeze thaw Zinc oxide Polyvinyl alcohol