Application of activity-based protein profiling to study enzyme function in adipocytes.
Activity-based protein profiling (ABPP) is a chemical proteomics approach that utilizes small-molecule probes to determine the functional state of enzymes directly in native systems. ABPP probes selectively label active enzymes, but not their inactive forms, facilitating the characterization of changes in enzyme activity that occur without alterations in protein levels. ABPP can be a tool superior to conventional gene expression and proteomic profiling methods to discover new enzymes active in adipocytes and to detect differences in the activity of characterized enzymes that may be associated with disorders of adipose tissue function. ABPP probes have been developed that react selectively with most members of specific enzyme classes. Here, using as an example the serine hydrolase family that includes many enzymes with critical roles in adipocyte physiology, we describe methods to apply ABPP analysis to the study of adipocyte enzymatic pathways.
['Galmozzi|Andrea|A|', 'Dominguez|Eduardo|E|', 'Cravatt|Benjamin F|BF|', 'Saez|Enrique|E|']
D017667:Adipocytes D000818:Animals D057075:Enzyme Assays D006801:Humans D015336:Molecular Probe Techniques D020543:Proteome D040901:Proteomics D057057:Serine Proteases
Activity-based protein profiling ABPP probes Enzyme activity Serine hydrolases Adipocyte enzymes Chemoproteomics methods Functional proteomics