NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.
RNA-binding proteins (RBPs) are significantly dysregulated in glioma. In this study, we demonstrated the upregulation of Nuclear cap-binding subunit 3 (NCBP3) in glioma tissues and cells. Further, knockdown of NCBP3 inhibited the malignant progression of glioma. NCBP3 directly bound to small nucleolar RNA host gene 6 (SNHG6) and stabilized SNHG6 expression. In contrast, the gastrulation brain homeobox 2 (GBX2) transcription factor was downregulated in glioma tissues and cells. SNHG6 inhibited GBX2 transcription by mediating the H3K27me3 modification induced by polycomb repressive complex 2 (PRC2). Moreover, GBX2 decreased the promoter activities and downregulated the expression of the flotillin protein family 1 (FLOT1) oncogene. In conclusion, NCBP3/SNHG6 inhibits GBX2 transcription in a PRC2-dependent manner to facilitate the malignant progression of gliomas.
['Li|Xiwen|X|', 'Zhang|Fangfang|F|', 'Ma|Jun|J|', 'Ruan|Xuelei|X|', 'Liu|Xiaobai|X|', 'Zheng|Jian|J|', 'Liu|Yunhui|Y|', 'Cao|Shuo|S|', 'Shen|Shuyuan|S|', 'Shao|Lianqi|L|', 'Cai|Heng|H|', 'Li|Zhen|Z|', 'Xue|Yixue|Y|']
D045744:Cell Line, Tumor D018450:Disease Progression D015972:Gene Expression Regulation, Neoplastic D055785:Gene Knockdown Techniques D005910:Glioma D006657:Histones D018398:Homeodomain Proteins D006801:Humans D060787:Neoplasm Grading D009367:Neoplasm Staging D011401:Promoter Regions, Genetic D011485:Protein Binding D034622:RNA Interference D062085:RNA, Long Noncoding D014158:Transcription, Genetic D025521:Tumor Suppressor Proteins
glioma RNA-binding proteins H3K27me3 long non-coding RNA