Antibiotic resistances of intestinal lactobacilli isolated from wild boars.
Acquired antibiotic resistances have been reported in lactobacilli of various animal and food sources, but there are no data from wild boar. The objective was a preliminary examination of the antibiotic resistance prevalence of intrinsically vancomycin-resistant lactobacilli isolated from wild boar intestines and analysis of the genetic determinants implicated. Out of three wild boars, 121 lactobacilli were recovered and grouped according to their whole cell protein patterns. Initial phenotypic screening revealed that all were susceptible to erythromycin (2 μg/ml), but 30 were resistant to tetracycline (32 μg/ml). Based on Randomly Amplified Polymorphic DNA-PCR clustering, 64 strains were selected as representative genotypes for identification and minimum inhibitory concentration (MIC) determination. Partial 16S rRNA gene sequencing identified four species: (i) L. mucosae (n=57), (ii) L. reuteri (n=47), (iii) L. fermentum (n=12), and (iv) L. murinus (n=5). Most heterofermentative strains displayed low MICs for ampicillin (AMP), chloramphenicol (CHL), streptomycin (STR), kanamycin (KAN), gentamicin (GEN), erythromycin (ERY), quinupristin/dalfopristin (Q/D), and clindamycin (CLI). Atypical MICs were found mainly in L. mucosae and L. reuteri for TET, KAN, STR, AMP and CHL, but except the TET MICs of L. mucosae mostly at low level. L. murinus strains revealed atypical MICs for aminoglycosides, and/or CHL, AMP, CLI. PCR screening detected tet(W) in 12 and tet(M) in one of heterofermentative strains, as well as the aph(3')-III kanamycin gene in L. murinus. This is the first report showing acquired antibiotic resistance determinants in intestinal lactobacilli of wild boar origin.
['Klose|Viviana|V|', 'Bayer|Katharina|K|', 'Kern|Corinna|C|', 'Goelß|Florian|F|', 'Fibi|Silvia|S|', 'Wegl|Gertrude|G|']
D000818:Animals D000900:Anti-Bacterial Agents D004352:Drug Resistance, Microbial D005838:Genotype D007422:Intestines D007778:Lactobacillus D008826:Microbial Sensitivity Tests D016133:Polymerase Chain Reaction D012336:RNA, Ribosomal, 16S D019105:Random Amplified Polymorphic DNA Technique D034421:Sus scrofa
Antibiotic resistances Lactobacilli Wild boar intestine