Cryotherapy: A Novel Method for Virus Eradication in Economically Important Plant Species.
Virus diseases have been a great threat to production of economically important crops. In practice, the use of virus-free planting material is an effective strategy to control viral diseases. Cryotherapy, developed based on cryopreservation, is a novel plant biotechnology tool for virus eradication. Comparing to the traditional meristem culture for virus elimination, cryotherapy resulted in high efficiency of pathogen eradication. In general, cryotherapy includes seven major steps: (1) introduction of infected plant materials into in vitro cultures, (2) shoot tip excision, (3) tolerance induction of explants to dehydration and subsequent freezing in liquid nitrogen (LN), (4) a short-time treatment of explants in LN, (5) warming and post-culture for regeneration, (6) re-establishment of regenerated plants in greenhouse conditions, and (7) virus indexing.
['Wang|Min-Rui|MR|', 'Chen|Long|L|', 'Zhang|Zhibo|Z|', 'Blystad|Dag-Ragnar|DR|', 'Wang|Qiao-Chun|QC|']
D018556:Crops, Agricultural D015925:Cryopreservation D004587:Electrophoresis, Agar Gel D010935:Plant Diseases D018520:Plant Shoots D010942:Plant Viruses D060888:Real-Time Polymerase Chain Reaction D012987:Soil
Cryotherapy Virus eradication Shoot tips Droplet-vitrification Encapsulation-dehydration RT-PCR