GhCaM7-like, a calcium sensor gene, influences cotton fiber elongation and biomass production.
Calcium signaling regulates many developmental processes in plants. Calmodulin (CaM) is one of the most conserved calcium sensors and has a flexible conformation in eukaryotes. The molecular functions of CaM are unknown in cotton, which is a major source of natural fiber. In this study, a Gossypium hirsutum L.CaM7-like gene was isolated from upland cotton. Bioinformatics analysis indicated that the GhCaM7-like gene was highly conserved as compared with Arabidopsis AtCaM7. The GhCaM7-like gene showed a high expression level in elongating fibers. Expression of β-glucuronidase was observed in trichomes on the stem, leaf and root in transgenic Arabidopsis plants of a PROGhCaM7-like:GUS fusion. Silencing of the GhCaM7-like gene resulted in decreased fiber length, but also caused reduction in stem height, leaf dimensions, seed length and 100-seed weight, in comparison with those of the control. Reduced expression of the GhCaM7-like gene caused decreased Ca2+ influx in cells of the leaf hypodermis and stem apex, and down-regulation of GhIQD1 (IQ67-domain containing protein), GhAnn2 (Annexins) and GhEXP2 (Expansin). These results indicate that the GhCaM7-like gene plays a vital role in calcium signaling pathways, and may regulate cotton fiber elongation and biomass production by affecting Ca2+ signatures and downstream signaling pathways of CaM.
['Cheng|Yuan|Y|', 'Lu|Lili|L|', 'Yang|Zhaoen|Z|', 'Wu|Zhixia|Z|', 'Qin|Wenqiang|W|', 'Yu|Daoqian|D|', 'Ren|Zhongying|Z|', 'Li|Yi|Y|', 'Wang|Lingling|L|', 'Li|Fuguang|F|', 'Yang|Zuoren|Z|']
D000595:Amino Acid Sequence D029681:Arabidopsis Proteins D018533:Biomass D002118:Calcium D020013:Calcium Signaling D002147:Calmodulin D032561:Cotton Fiber D018507:Gene Expression Regulation, Developmental D018506:Gene Expression Regulation, Plant D020868:Gene Silencing D003368:Gossypium D010802:Phylogeny D018515:Plant Leaves D010940:Plant Proteins D018547:Plant Stems D030821:Plants, Genetically Modified D020133:Reverse Transcriptase Polymerase Chain Reaction D012639:Seeds D017386:Sequence Homology, Amino Acid
GhCaM7-like Fiber elongation Biomass production Calmodulin