Astrocyte Infection during Rabies Encephalitis Depends on the Virus Strain and Infection Route as Demonstrated by Novel Quantitative 3D Analysis of Cell Tropism.
Although conventional immunohistochemistry for neurotropic rabies virus (RABV) usually shows high preference for neurons, non-neuronal cells are also potential targets, and abortive astrocyte infection is considered a main trigger of innate immunity in the CNS. While in vitro studies indicated differences between field and less virulent lab-adapted RABVs, a systematic, quantitative comparison of astrocyte tropism in vivo is lacking. Here, solvent-based tissue clearing was used to measure RABV cell tropism in infected brains. Immunofluorescence analysis of 1 mm-thick tissue slices enabled 3D-segmentation and quantification of astrocyte and neuron infection frequencies. Comparison of three highly virulent field virus clones from fox, dog, and raccoon with three lab-adapted strains revealed remarkable differences in the ability to infect astrocytes in vivo. While all viruses and infection routes led to neuron infection frequencies between 7-19%, striking differences appeared for astrocytes. Whereas astrocyte infection by field viruses was detected independent of the inoculation route (8-27%), only one lab-adapted strain infected astrocytes route-dependently [0% after intramuscular (i.m.) and 13% after intracerebral (i.c.) inoculation]. Two lab-adapted vaccine viruses lacked astrocyte infection altogether (0%, i.c. and i.m.). This suggests a model in which the ability to establish productive astrocyte infection in vivo functionally distinguishes field and attenuated lab RABV strains.
['Potratz|Madlin|M|', 'Zaeck|Luca|L|', 'Christen|Michael|M|', 'Te Kamp|Verena|V|', 'Klein|Antonia|A|', 'Nolden|Tobias|T|', 'Freuling|Conrad M|CM|', 'Müller|Thomas|T|', 'Finke|Stefan|S|']
D000818:Animals D001253:Astrocytes D001921:Brain D004285:Dogs D004660:Encephalitis D006801:Humans D007113:Immunity, Innate D009474:Neurons D011818:Rabies D011820:Rabies virus D056189:Viral Tropism
astrocyte infection rabies 3D imaging rabies pathogenicity uDISCO