Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh.
Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing from tissue-extracted genomic DNA detected new CPV-2a as the contributing antigenic variant. Further genotyping using polymerase chain reaction coupled with high-resolution melt assays (PCR-HRM) confirmed new CPV-2a infection in all deceased dogs. PCR-HRM of additional thirty-four clinically suspected dogs suggested that this variant is in wider community circulation, at least in the southeastern part of Bangladesh. We present complete genome sequence of the new CPV-2a variant circulating in the domestic canine population of Bangladesh.
['Rakib|Tofazzal Md|TM|0000-0003-2642-9908', 'Nath|Babu Kanti|BK|', 'Das|Tridip|T|', 'Yadav|Saroj Kumar|SK|0000-0002-6642-1719', 'Raidal|Shane R|SR|', 'Das|Shubhagata|S|']
canine parvovirus whole genome shotgun sequencing new CPV-2a PCR-HRM