ADP is a vasodilator component from Lasiodora sp. mygalomorph spider venom.
Members of the spider genus Lasiodora are widely distributed in Brazil, where they are commonly known as caranguejeiras. Lasiodora spider venom is slightly harmful to humans. The bite of this spider causes local pain, edema and erythema. However, Lasiodora sp. spider venom may be a source of important pharmacological tools. Our research group has described previously that Lasiodora sp. venom produces bradycardia in the isolated rat heart. In the present work, we sought to evaluate the vascular effect of Lasiodora sp. venom and to isolate the vasoactive compounds from the venom. The results showed that Lasiodora spider venom induced a concentration-dependent vasodilation in rat aortic rings, which was dependent on the presence of a functional endothelium and abolished by the nitric oxide synthase (NOS) inhibitor L-NAME. Western blot experiments revealed that the venom also increased endothelial NOS function by increasing phosphorylation of the Ser¹¹⁷⁷ residue. Assay-directed fractionation isolated a vasoactive fraction from Lasiodora sp. venom. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) assays identified a mixture of two compounds: adenosine diphosphate (ADP, approximately 90%) and adenosine monophosphate (AMP, approximately 10%). The vasodilator effects of Lasiodora sp. whole venom, as well as ADP, were significantly inhibited by suramin, which is a purinergic P2-receptor antagonist. Therefore, the results of the present work indicate that ADP is a main vasodilator component of Lasiodora sp. spider venom.
['Horta|C C|CC|', 'Rezende|B A|BA|', 'Oliveira-Mendes|B B R|BB|', 'Carmo|A O|AO|', 'Capettini|L S A|LS|', 'Silva|J F|JF|', 'Gomes|M T|MT|', 'Chávez-Olórtegui|C|C|', 'Bravo|C E S|CE|', 'Lemos|V S|VS|', 'Kalapothakis|E|E|']
D000244:Adenosine Diphosphate D000249:Adenosine Monophosphate D000818:Animals D015153:Blotting, Western D005591:Chemical Fractionation D004727:Endothelium D066298:In Vitro Techniques D013058:Mass Spectrometry D019331:NG-Nitroarginine Methyl Ester D019001:Nitric Oxide Synthase D019906:Nuclear Magnetic Resonance, Biomolecular D010766:Phosphorylation D051381:Rats D013111:Spider Venoms D013112:Spiders D013498:Suramin D014664:Vasodilation D014665:Vasodilator Agents
ADP Vasodilation Lasiodora Spider Venom Nitric oxide