In vitro evaluation of the potential therapeutic role of Dendropanax morbifera extract in ameliorating osteoporosis and resultant bone impairment using MC3T3-E1 cells.
Osteoporosis is a widespread musculoskeletal deformity that affects thousands of older people every year, leading to bone abnormalities and ultimately increasing the risk of bone fractures in both genders. It is considered a lethal disease causing death in thousands of people at the late stage of life. Dendropanax morbifera Leveille is a subtropical broad-leaved prevalent species in Korea. Extracts of the leaves, stems, roots, and seeds of D. morbifera have been used in traditional medicine for the treatment of numerous diseases such as diabetes, atherogenesis, skin disorders, and headaches. However, the anti-osteoporosis effects of D. morbifera have not been examined. The primary objectives of this study were to elucidate the anti-osteoporosis effect of D. morbifera extract through an in vitro study using pre-osteoblastic MC3T3-E1 cells. We found that D. morbifera strongly increased the expression of bone metabolic markers such as alkaline phosphatase (ALP) activity, type I collagen (Col-I) level, and mineralization. Additionally, D. morbifera extract also upregulated the mRNA expression levels of osteogenic genes including ALP, osteocalcin (OCN), osterix (Osx), and runt-related transcription factor 2 (Runx2) in MC3T3-E1 cells via upregulation of bone morphogenetic protein 2 (BMP-2)/p38 MAPK/JNK and Smad1/5/8 signaling pathways. Moreover, addition of D. morbifera significantly suppressed the inhibitory effect of SB203580 (p38 inhibitor). In conclusion, the current study demonstrated that D. morbifera extract significantly increased osteoblast differentiation and mineralization in MC3T3-E1 cells by regulating BMP-2/p38/JNK and Smad1/5/8. Our study might be helpful in the discovery and development of new anti-osteoporosis therapeutic agents.
['Yang|Dong-Uk|DU|', 'Siddiqi|Muhammad Hanif|MH|', 'Ahn|Sungeun|S|', 'Kang|Sera|S|', 'Noh|Hae-Yong|HY|', 'Yang|Deok-Chun|DC|']
D016475:3T3 Cells D000818:Animals D027481:Araliaceae D049109:Cell Proliferation D050659:Core Binding Factor Alpha 1 Subunit D018507:Gene Expression Regulation, Developmental D051379:Mice D010006:Osteoblasts D015675:Osteocalcin D010012:Osteogenesis D010024:Osteoporosis D010936:Plant Extracts D015398:Signal Transduction D048051:p38 Mitogen-Activated Protein Kinases
Dendropanax Osteoporosis MC3T3-E1 cells p38 Osteoblasts BMP-2