Overexpression of CENPF is associated with progression and poor prognosis of lung adenocarcinoma.
Background and aim: The molecular signatures of lung adenocarcinoma (LUAD) are not well understood. Centromere protein F (CENPF) has been shown to promote oncogenesis in many cancers; however, its role in LUAD has not been illustrated. We explored the role of CENPF in LUAD. Methods: CENPF expression level was investigated in public online database firstly, the prognosis of CENPF in LUAD were also assessed by Kaplan-Meier analysis. Then quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed using 13 matched pairs of clinical LUAD tissue samples. Subsequently, the impact of CENPF expression on cell proliferation, cell cycle, apoptosis, colony formation was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), flow cytometric analysis and colony formation assay, respectively. Finally, experimental xenograft lung cancer model of nude mice armpit of right forelimb to determine the effect of CENPF on LUAD tumorigenesis. Results: CENPF mRNA expression was significantly elevated in LUAD tissues compared with adjacent non-tumor lung tissues in Gene Expression Profiling Interactive Analysis (GEPIA) (P < 0.001). Up-regulated CENPF was remarkably positively associated with pathological stage, relapse free survival (RFS) as well as overall survival (OS) of LUAD patients. Besides, CENPF knockdown greatly suppressed A549 cell proliferation, induced S phase arrest, promoted apoptosis and decreased colony numbers of LUAD cells. Furthermore, knockdown of CENPF significantly inhibited the tumor growth of the LUAD cells in an experimental xenograft lung cancer model of nude mice armpit of right forelimb. Conclusion: Taken together, these results demonstrated that CENPF may serve as a potential biomarker of prognostic relevance and a potential therapeutic target for LUAD.
['Li|Mei-Xiang|MX|', 'Zhang|Meng-Yu|MY|', 'Dong|Huan-Huan|HH|', 'Li|Ai-Jun|AJ|', 'Teng|Hai-Feng|HF|', 'Liu|Ai-Ling|AL|', 'Xu|Ning|N|', 'Qu|Yi-Qing|YQ|']
D000077192:Adenocarcinoma of Lung D000818:Animals D014408:Biomarkers, Tumor D045744:Cell Line, Tumor D049109:Cell Proliferation D002868:Chromosomal Proteins, Non-Histone D066264:Datasets as Topic D018450:Disease Progression D018572:Disease-Free Survival D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D053208:Kaplan-Meier Estimate D008168:Lung D008175:Lung Neoplasms D051379:Mice D008840:Microfilament Proteins D008875:Middle Aged D009364:Neoplasm Recurrence, Local D011379:Prognosis D023041:Xenograft Model Antitumor Assays
CENPF LUAD cell cycle prognostic biomarker