A transgenic system for targeted ablation of reproductive and maternal-effect genes.
Maternally provided gene products regulate the earliest events of embryonic life, including formation of the oocyte that will develop into an egg, and eventually into an embryo. Forward genetic screens have provided invaluable insights into the molecular regulation of embryonic development, including the essential contributions of some genes whose products must be provided to the transcriptionally silent early embryo for normal embryogenesis, called maternal-effect genes. However, other maternal-effect genes are not accessible due to their essential zygotic functions during embryonic development. Identifying these regulators is essential to fill the large gaps in our understanding of the mechanisms and molecular pathways contributing to fertility and to maternally regulated developmental processes. To identify these maternal factors, it is necessary to bypass the earlier requirement for these genes so that their potential later functions can be investigated. Here, we report reverse genetic systems to identify genes with essential roles in zebrafish reproductive and maternal-effect processes. As proof of principle and to assess the efficiency and robustness of mutagenesis, we used these transgenic systems to disrupt two genes with known maternal-effect functions: kif5ba and bucky ball.
['Bertho|Sylvain|S|', 'Kaufman|Odelya|O|', 'Lee|KathyAnn|K|', 'Santos-Ledo|Adrian|A|', 'Dellal|Daniel|D|', 'Marlow|Florence L|FL|0000-0002-9362-0342']
D000818:Animals D030801:Animals, Genetically Modified D047108:Embryonic Development D015870:Gene Expression D055785:Gene Knockdown Techniques D018390:Gene Targeting D005822:Genetic Vectors D005854:Germ Cells D006801:Humans D000072741:Maternal Inheritance D016296:Mutagenesis D015203:Reproducibility of Results D012098:Reproduction D019076:Transgenes
Mutagenesis Germ cells Maternal effect