Biochemical Characterization of Echinococcus multilocularis Antigen B3 Reveals Insight into Adaptation and Maintenance of Parasitic Homeostasis at the Host-Parasite Interface.
Alveolar echinococcosis (AE) caused by Echinococcus multilocularis metacestode is frequently associated with deleterious zoonotic helminthiasis. The growth patterns and morphological features of AE, such as invasion of the liver parenchyme and multiplication into multivesiculated masses, are similar to those of malignant tumors. AE has been increasingly detected in several regions of Europe, North America, Central Asia, and northwestern China. An isoform of E. multilocularis antigen B3 (EmAgB3) shows a specific immunoreactivity against patient sera of active-stage AE, suggesting that EmAgB3 might play important roles during adaptation of the parasite to hosts. However, expression patterns and biochemical properties of EmAgB3 remained elusive. The protein profile and nature of component proteins of E. multilocularis hydatid fluid (EmHF) have never been addressed. In this study, we conducted proteome analysis of EmHF of AE cysts harvested from immunocompetent mice. We observed the molecular and biochemical properties of EmAgB3, including differential transcription patterns of paralogous genes, macromolecular protein status by self-assembly, distinct oligomeric states according to individual anatomical compartments of the worm, and hydrophobic ligand-binding protein activity. We also demonstrated tissue expression patterns of EmAgB3 transcript and protein. EmAgB3 might participate in immune response and recruitment of essential host lipids at the host-parasite interface. Our results might contribute to an in depth understanding of the biophysical and biological features of EmAgB3, thus providing insights into the design of novel targets to control AE.
['Ahn|Chun-Seob|CS|', 'Kim|Jeong-Geun|JG|', 'Han|Xiumin|X|', 'Bae|Young-An|YA|', 'Park|Woo-Jae|WJ|', 'Kang|Insug|I|', 'Wang|Hu|H|', 'Kong|Yoon|Y|0000-0003-0905-4606']
D000222:Adaptation, Physiological D000818:Animals D003001:Cloning, Molecular D004195:Disease Models, Animal D004443:Echinococcosis D004444:Echinococcosis, Hepatic D048210:Echinococcus multilocularis D004926:Escherichia coli D005260:Female D015870:Gene Expression D005786:Gene Expression Regulation D015801:Helminth Proteins D006706:Homeostasis D006790:Host-Parasite Interactions D006801:Humans D008074:Lipoproteins D051379:Mice D019301:Oleic Acid D020543:Proteome D011994:Recombinant Proteins D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Echinococcus multilocularis antigen B3 alveolar echinococcosis hydatid fluid proteome hydrophobic ligand-binding protein expressional regulation multimeric states