Lipoprotein lipase does not increase significantly in the postprandial plasma.
BACKGROUND
Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake.


METHODS
Standard meal (n=81), 50g of fat (n=54), 75g of glucose (n=25) and cookie (25g fat and 75g carbohydrate fat) (n=28) were administered in generally healthy volunteers. Plasma LPL, HTGL and TC, TG, LDL-C, HDL-C, RLP-C and RLP-TG were determined at subsequent withdrawal after the food intake.


RESULTS
Plasma TG, RLP-C and RLP-TG were significantly increased at 8PM (2h after dinner of standard meal) compared with 8AM before breakfast within the same day. Also those parameters were significantly increased in 2-6h after fat load. However, the concentrations and activities of LPL and HTGL did not significantly increase in association with an increase in the TG and remnant lipoproteins. Also LPL concentration did not significantly increase after glucose and "cookie test" within 4h.


CONCLUSION
No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.
['Ishiyama|Nobuyoshi|N|', 'Sakamaki|Kouji|K|', 'Shimomura|Younosuke|Y|', 'Kotani|Kazuhiko|K|', 'Tsuzaki|Kokoro|K|', 'Sakane|Naoki|N|', 'Miyashita|Kazuya|K|', 'Fukamachi|Isao|I|', 'Kobayashi|Junji|J|', 'Stanhope|Kimber L|KL|', 'Havel|Peter J|PJ|', 'Kamachi|Keiko|K|', 'Tanaka|Akira|A|', 'Tokita|Yoshiharu|Y|', 'Machida|Tetsuo|T|', 'Murakami|Masami|M|', 'Nakajima|Katsuyuki|K|']
D000293:Adolescent D000328:Adult D004040:Dietary Carbohydrates D004041:Dietary Fats D004347:Drug Interactions D004435:Eating D005215:Fasting D005260:Female D006801:Humans D008049:Lipase D008071:Lipoprotein Lipase D008297:Male D019518:Postprandial Period D055815:Young Adult
Lipoprotein lipase RLP-C RLP-TG Remnant lipoproteins Chylomicrons Hepatic triglyceride lipase Triglycerides Very low density lipoproteins