Association between C677T polymorphism of MTHFR gene and risk of amyotrophic lateral sclerosis: Polish population study and a meta-analysis.
OBJECTIVE
Genetic factors play a role in pathogenesis of amyotrophic lateral sclerosis (ALS). A few studies demonstrated that the TT genotype of C677T polymorphism of the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene can increase the risk of sporadic ALS. The aim of our study was to determine the relationship between C677T polymorphism of MTHFR gene and the risk of sporadic ALS in Polish population and to perform the meta-analysis assessing the significance this polymorphism for the risk of ALS in Caucasian population.


METHODS
We included 251 patients with ALS and 500 control subjects recruited from Polish population and performed the meta-analysis of published data from Caucasian population. MTHFR C677T polymorphism was genotyped using a TaqMan assay and 7900HT Fast real Time PCR System.


RESULTS
The frequency of genotypes did not differ significantly between Polish ALS patients and control subjects (CC: 45.0 vs 45.8%, CT: 48.2 vs 45.0%, TT: 6.8 vs 9.2%, P=0.46). The meta-analysis including 863 ALS patients and 1362 controls revealed that TT genotype increases the risk of sporadic ALS in Caucasian population.


CONCLUSION
Although we did not find the association between C677T polymorphism of MTHRF gene and risk of ALS in Polish population, the results of meta-analysis suggest that the TT genotype can be a genetic risk factor for ALS in Caucasian population.
['Żur-Wyrozumska|Kamila|K|', 'Pera|Joanna|J|', 'Dziubek|Anna|A|', 'Sado|Małgorzata|M|', 'Golenia|Aleksandra|A|', 'Słowik|Agnieszka|A|', 'Dziedzic|Tomasz|T|']
D000328:Adult D000368:Aged D000483:Alleles D000690:Amyotrophic Lateral Sclerosis D005260:Female D005787:Gene Frequency D020022:Genetic Predisposition to Disease D005838:Genotype D006801:Humans D008297:Male D042965:Methylenetetrahydrofolate Reductase (NADPH2) D008875:Middle Aged D011044:Poland D011110:Polymorphism, Genetic D012306:Risk
Polymorphism Amyotrophic lateral sclerosis Genetics 5,10-Methylenetetrahydrofolate reductase