Antibiotic susceptibilities and genetic variations in macrolide resistance genes of Ureaplasma spp. isolated in China.
Macrolides are widely used for the treatment of Ureaplasma spp. infection. The aim of this study was to investigate possible genetic resistance determinants in Ureaplasma spp. isolates. A total of eleven macrolide- resistant Ureaplasma spp. isolates, recovered from urogenital specimens, were investigated for genetic mechanisms of macrolide resistance. The 23S rRNA operons, as well as L4 and L22 ribosomal protein genes, were amplified and sequenced. Our study identified that the mutation A2066G in the 23S rRNA and four mutations (G361T, A406G, C422T and G196A) in the L22 ribosomal protein, may be responsible for the resistance of Ureaplasma spp. to macrolides, in China.
['Song|Tiejun|T|', 'Huang|Jun|J|', 'Liu|Zhiwei|Z|', 'Zhang|Ying|Y|', 'Kong|Yingying|Y|', 'Ruan|Zhi|Z|']
D000900:Anti-Bacterial Agents D002681:China D024881:Drug Resistance, Bacterial D014644:Genetic Variation D018942:Macrolides D008826:Microbial Sensitivity Tests D009154:Mutation D012338:RNA, Ribosomal, 23S D012269:Ribosomal Proteins D014509:Ureaplasma
genetic variation macrolide resistance Ureaplasma spp. 23S rRNA ribosomal proteins