A new approach to recycling cephalosporin fermentation residue into plant biostimulants.
Cephalosporin fermentation residue (CFR), a byproduct of the pharmaceutical industry, mainly contains the mycelial biomass and unutilized culture medium, which can be reused as a high-quality protein source. This study first reports the recycling CFR into plant biostimulants using partial acid hydrolysis. Temperature, reaction time and ratio of hydrochloric acid/dry matter (H/S) were optimized for yielding both free amino acid and low molecular weight fraction based on response surface methodology. The crude protein concentration of CFR is 55%, with glutamic acid being the dominant amino acid (12.5%). Two favorable hydrolysis conditions were obtained: (1) 140 °C, 10.7 h, 17 H/S for maximizing the amino acid yield of 45.5 g/100 g CFR dw and (2) 100 °C, 10.7 h, 19 H/S for maximizing the low molecular weight fraction of 28.2%. The CFR-derived biostimulants obtained from two optimum conditions possessed two biostimulant modes of action: plant growth promotors/inhibitors and stress alleviators. However, they showed the differences in aminograms and profiles of low molecular weight compounds. Neither residual cephalosporin C nor its byproduct was detected in the CFR-derived biostimulants, suggesting that partial acid hydrolysis appears capable of recycling CFR into plant biostimulant safely.
['Cai|Chen|C|', 'Hua|Yu|Y|', 'Liu|Huiling|H|', 'Dai|Xiaohu|X|']
D018533:Biomass D002511:Cephalosporins D005285:Fermentation D006868:Hydrolysis D013696:Temperature
Cephalosporin fermentation residue Biostimulants Partial acid hydrolysis Amino acid Response surface methodology