Effect of Paclitaxel/etoposide co-loaded polymeric nanoparticles on tumor size and survival rate in a rat model of glioblastoma.
The aim of this work is to co-load paclitaxel (PTX) and etoposide (ETP) in methoxy poly(ethylene glycol)-poly(lactic-co-glycolic acid) nanoparticles (mPEG-PLGA NPs) to overcome pharmacokinetics and physiological limitations and enhance therapeutic efficacy for treating intracranial glioblastoma. Both drugs were loaded into mPEG-PLGA NPs by a nano-precipitation method. The resultant NPs demonstrated an enhanced cytotoxic effect indicated by lower IC50 values and augmented cell apoptosis to U87 and C6 glioma cell lines compared to both free drugs. Additionally, blood compatibility assays showed that the PTX/ETP co-loaded mPEG-PLGA NPs did not induce blood hemolysis, blood clotting, or platelet aggregation. In vivo anti-glioma efficacy evaluation in rats bearingintracranialC6glioma revealed a superior anti-glioma activity for the treatment with PTX/ETP co-loaded mPEG-PLGA NPs compared to other formulations, particularly a significantly longer median survival, 76 days compared to 36 days for free PTX and 37 days for free ETP treatment, respectively, and higher tumor regression, proved by magnetic resonance imaging (MRI).
['Maleki|Hassan|H|', 'Hosseini Najafabadi|Mohammad Reza|MR|', 'Webster|Thomas J|TJ|', 'Hadjighassem|Mahmoud Reza|MR|', 'Sadroddiny|Esmaeil|E|', 'Ghanbari|Hossein|H|', 'Khosravani|Masood|M|', 'Adabi|Mahdi|M|']
D000818:Animals D045744:Cell Line, Tumor D004337:Drug Carriers D005047:Etoposide D005909:Glioblastoma D053758:Nanoparticles D017239:Paclitaxel D011092:Polyethylene Glycols D051381:Rats D015996:Survival Rate
Paclitaxel Etoposide Nanoparticle Glioblastoma mPEG-PLGA Chemotherapy