Breath analysis by two-dimensional gas chromatography with dual flame ionisation and mass spectrometric detection - Method optimisation and integration within a large-scale clinical study.
Precision medicine has spurred new innovations in molecular pathology leading to recent advances in the analysis of exhaled breath as a non-invasive diagnostic tool. Volatile organic compounds (VOCs) detected in exhaled breath have the potential to reveal a wealth of chemical and metabolomic information. This study describes the development of a method for the analysis of breath, based on automated thermal desorption (TD) combined with flow modulated comprehensive two-dimensional gas chromatography (GC×GC) with dual flame ionisation and quadrupole mass spectrometric detection (FID and qMS). The constrained optimisation and analytical protocol was designed to meet the practical demands of a large-scale multi-site clinical study, while maintaining analytical rigour to produce high fidelity data. The results demonstrate a comprehensive method optimisation for the collection and analysis of breath VOCs by GC×GC, integral to the standardisation and integration of breath analysis within large clinical studies.
['Wilde|Michael J|MJ|', 'Cordell|Rebecca L|RL|', 'Salman|Dahlia|D|', 'Zhao|Bo|B|', 'Ibrahim|Wadah|W|', 'Bryant|Luke|L|', 'Ruszkiewicz|Dorota|D|', 'Singapuri|Amisha|A|', 'Free|Robert C|RC|', 'Gaillard|Erol A|EA|', 'Beardsmore|Caroline|C|', 'Thomas|C L Paul|CLP|', 'Brightling|Chris E|CE|', 'Siddiqui|Salman|S|', 'Monks|Paul S|PS|']
D001944:Breath Tests D000068456:Clinical Studies as Topic D005410:Flame Ionization D008401:Gas Chromatography-Mass Spectrometry D006801:Humans D012015:Reference Standards D055549:Volatile Organic Compounds
Breath analysis Precision medicine Metabolomics Flow modulation Comprehensive two-dimensional gas chromatography GC×GC