Whey Protein Concentrate WPC-80 Intensifies Glycoconjugate Catabolism and Induces Oxidative Stress in the Liver of Rats.
The aim of this study was to evaluate the effect of whey protein concentrate (WPC-80) on glycoconjugate catabolism, selected markers of oxidative stress and liver inflammation. The experiment was conducted on male Wistar rats (n = 63). The animals from the study group were administered WPC-80 at a dose of 0.3 or 0.5 g/kg body weight for 7, 14 or 21 days, while rats from the control group received only 0.9% NaCl. In liver homogenates, we assayed the activity of N-acetyl-β-D-hexosaminidase (HEX), β-glucuronidase (GLU), β-galactosidase (GAL), α-mannosidase (MAN), α-fucosidase (FUC), as well as the level of reduced glutathione (GSH), malondialdehyde (MDA), interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1). A significantly higher activity of HEX, GLU, MAN and FUC were found in the livers of rats receiving WPC-80 compared to controls. Serum ALT and AST were significantly higher in the animals supplemented with WPC-80 at a dose of 0.5 g/kg body weight for 21 days. In the same group of animals, enhanced level of GSH, MDA, IL-1β and TGF-β1 were also observed. WPC-80 is responsible for intensive remodelling of liver tissue and induction of oxidative stress especially at a dose of 0.5 g/kg body weight.
['Żebrowska-Gamdzyk|Marta|M|', 'Maciejczyk|Mateusz|M|0000-0001-5609-3187', 'Zalewska|Anna|A|', 'Guzińska-Ustymowicz|Katarzyna|K|', 'Tokajuk|Anna|A|', 'Car|Halina|H|']
D000818:Animals D015415:Biomarkers D016207:Cytokines D019587:Dietary Supplements D006001:Glycoconjugates D006026:Glycoside Hydrolases D018836:Inflammation Mediators D008099:Liver D008247:Lysosomes D008297:Male D018384:Oxidative Stress D017208:Rats, Wistar D000067816:Whey Proteins
whey oxidative stress liver exoglycosidases