Solid Lipid Nanoparticles of Albendazole for Enhancing Cellular Uptake and Cytotoxicity against U-87 MG Glioma Cell Lines.
Albendazole (ABZ) is an antihelminthic drug used for the treatment of several parasitic infestations. In addition to this, there are reports on the anticancer activity of ABZ against a wide range of cancer types. However, its effect on glioma has not yet been reported. In the present study, cytotoxicity of ABZ and ABZ loaded solid lipid nanoparticles (ASLNs) was tested in human glioma/astrocytoma cell line (U-87 MG). Using glyceryl trimyristate as lipid carrier and tween 80 as surfactant spherical ASLNs with an average size of 218.4 ± 5.1 nm were prepared by a combination of high shear homogenization and probe sonication methods. A biphasic in vitro release pattern of ABZ from ASLNs was observed, where 82% of ABZ was released in 24 h. In vitro cell line studies have shown that ABZ in the form of ASLNs was more cytotoxic (IC50 = 4.90 µg/mL) to U-87 MG cells compared to ABZ in the free form (IC50 = 13.30 µg/mL) due to the efficient uptake of the former by these cells.
['Marslin|Gregory|G|', 'Siram|Karthik|K|', 'Liu|Xiang|X|', 'Khandelwal|Vinoth Kumar Megraj|VKM|', 'Xiaolei|Shen|S|', 'Xiang|Wang|W|', 'Franklin|Gregory|G|0000-0002-6745-3528']
D015766:Albendazole D000970:Antineoplastic Agents D045744:Cell Line, Tumor D002470:Cell Survival D004305:Dose-Response Relationship, Drug D065546:Drug Liberation D006801:Humans D008055:Lipids D053758:Nanoparticles D012995:Solubility
solid lipid nanoparticles albendazole cellular uptake cytotoxicity U-87 MG cells