Structural and oxidative changes in the kidney of crucian carp induced by silicon-based quantum dots.
Silicon-based quantum dots were intraperitoneally injected in Carassius auratus gibelio specimens and, over one week, the effects on renal tissue were investigated by following their distribution and histological effects, as well as antioxidative system modifications. After three and seven days, detached epithelial cells from the basal lamina, dilated tubules and debris in the lumen of tubules were observed. At day 7, nephrogenesis was noticed. The reduced glutathione (GSH) concentration decreased in the first three days and started to rise later on. The superoxide dismutase (SOD) activity increased only after one week, whereas catalase (CAT) was up-regulated in a time-dependent manner. The activities of glutathione reductase (GR) and glutathione peroxidise (GPX) decreased dramatically by approximately 50% compared to control, whereas the glutathione-S-transferase (GST) and glucose-6-phosphate dehydrogenase (G6PDH) increased significantly after 3 and 7 days of treatment. Oxidative modifications of proteins and the time-dependent increase of Hsp70 expression were also registered. Our data suggest that silicon-based quantum dots induced oxidative stress followed by structural damages. However, renal tissue is capable of restoring its integrity by nephron development.
['Petrache|Sorina Nicoleta|SN|', 'Stanca|Loredana|L|', 'Serban|Andreea Iren|AI|', 'Sima|Cornelia|C|', 'Staicu|Andreia Cristina|AC|', 'Munteanu|Maria Cristina|MC|', 'Costache|Marieta|M|', 'Burlacu|Radu|R|', 'Zarnescu|Otilia|O|', 'Dinischiotu|Anca|A|']
D000818:Animals D002347:Carps D002374:Catalase D005978:Glutathione D005979:Glutathione Peroxidase D005980:Glutathione Reductase D005982:Glutathione Transferase D007668:Kidney D015227:Lipid Peroxidation D010084:Oxidation-Reduction D018384:Oxidative Stress D045663:Quantum Dots D012825:Silicon D013482:Superoxide Dismutase
quantum dots oxidative stress fish kidney