Statistical Analysis of Quantitative RT-PCR Results.
Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) represents a benchmark technology in the detection and quantification of mRNA. Yet, accurate results cannot be realized without proper statistical analysis of RT-PCR data. Here we examine some of the issues concerning RT-PCR experiments that would benefit from rigorous statistical treatment including normalization, quantification, efficiency estimation, and sample size calculations. Examples are used to illustrate the methods.
['Khan-Malek|Richard|R|', 'Wang|Ying|Y|']
D015233:Models, Statistical D012333:RNA, Messenger D020133:Reverse Transcriptase Polymerase Chain Reaction
Statistical analysis RT-PCR Relative quantification