Olive mill wastewater biodegradation potential of white-rot fungi--Mode of action of fungal culture extracts and effects of ligninolytic enzymes.
Forty-nine white-rot strains belonging to 38 species of Basidiomycota were evaluated for olive-mill wastewater (OMW) degradation. Almost all fungi caused high total phenolics (>60%) and color (⩽ 70%) reduction, while COD and phytotoxicity decreased to a lesser extent. Culture extracts from selected Agrocybe cylindracea, Inonotus andersonii, Pleurotus ostreatus and Trametes versicolor strains showed non-altered physicochemical and enzymatic activity profiles when applied to raw OMW in the presence or absence of commercial catalase, indicating no interaction of the latter with fungal enzymes and no competition for H2O2. Hydrogen peroxide's addition resulted in drastic OMW's decolorization, with no effect on phenolic content, suggesting that oxidation affects colored components, but not necessarily phenolics. When fungal extracts were heat-treated, no phenolics decrease was observed demonstrating thus their enzymatic rather than physicochemical oxidation. Laccases added to OMW were reversibly inhibited by the effluent's high phenolic load, while peroxidases were stable and active during the entire process.
['Ntougias|Spyridon|S|', 'Baldrian|Petr|P|', 'Ehaliotis|Constantinos|C|', 'Nerud|Frantisek|F|', 'Merhautová|Věra|V|', 'Zervakis|Georgios I|GI|']
D001673:Biodegradation, Environmental D057919:Biological Oxygen Demand Analysis D005658:Fungi D018525:Germination D007220:Industrial Waste D042845:Laccase D008031:Lignin D031658:Olea D010544:Peroxidases D010636:Phenols D020076:Pleurotus D012639:Seeds D062065:Waste Water D014934:Wood
Catalase Laccase Peroxidase Phenolic content reduction Wood-rot fungi