The Non-Homologous End Joining Protein PAXX Acts to Restrict HSV-1 Infection.
Herpes simplex virus 1 (HSV-1) has extensive interactions with the host DNA damage response (DDR) machinery that can be either detrimental or beneficial to the virus. Proteins in the homologous recombination pathway are known to be required for efficient replication of the viral genome, while different members of the classical non-homologous end-joining (c-NHEJ) pathway have opposing effects on HSV-1 infection. Here, we have investigated the role of the recently-discovered c-NHEJ component, PAXX (Paralogue of XRCC4 and XLF), which we found to be excluded from the nucleus during HSV-1 infection. We have established that cells lacking PAXX have an intact innate immune response to HSV-1 but show a defect in viral genome replication efficiency. Counterintuitively, PAXX-/- cells were able to produce greater numbers of infectious virions, indicating that PAXX acts to restrict HSV-1 infection in a manner that is different from other c-NHEJ factors.
['Trigg|Ben J|BJ|', 'Lauer|Katharina B|KB|', 'Fernandes Dos Santos|Paula|P|', 'Coleman|Heather|H|', 'Balmus|Gabriel|G|0000-0003-2872-4468', 'Mansur|Daniel S|DS|0000-0001-6773-9334', 'Ferguson|Brian J|BJ|0000-0002-6873-1032']
D000818:Animals D002460:Cell Line D059766:DNA End-Joining Repair D004268:DNA-Binding Proteins D005814:Genes, Viral D016679:Genome, Viral D006561:Herpes Simplex D018259:Herpesvirus 1, Human D006801:Humans D007372:Interferons D051379:Mice D014764:Viral Proteins D014771:Virion D014779:Virus Replication
PAXX HSV-1 herpes simplex virus 1 DNA damage response DDR c-NHEJ classical non-homologous end joining