Green tea catechins alone or in combination alter functional parameters of human neutrophils via suppressing the activation of TLR-4/NFκB p65 signal pathway.
The purpose of this study was to evaluate the potential of a mixture containing the four main catechins found in green tea, as well it separately, as modulators of the functional parameters of human neutrophils. The cells were obtained from peripheral blood of healthy individuals isolated and cultured with a mix: 30 μM of EGCG, 3 μM of EGC, 2 μM of ECG and 1.4 μM of EC, as well as each one alone. We evaluated the cytotoxicity of catechins, production of several reactive oxygen species (ROS), antioxidant enzymes (SOD, CAT, GPx and GR), Nrf2, TLR4/IKK/NFκB, CD11b mRNA levels, intracellular calcium release, chemotactic and phagocytic capacity, myeloperoxidase (MPO), and G6PDH activities, hypochlorous acid (HOCl) and pro-inflammatory cytokines release, protein levels of TLR4, p38 MAPK, iNOS and p-65 NFκB. The actions of the catechins were evidenced by the reduction in inflammatory parameters, including the suppression of TLR4, NFκB and iNOS protein expression, decreased release of TNF-α, IL-1β and IL-6, migration capacity, MPO activity and HOCl production and the suppression of ROS, nitric oxide and peroxynitrite production, while inducing antioxidant enzyme activities and Nrf2 mRNA levels, phagocytic capacity and calcium release. Our results demonstrate that catechins present marked immunomodulatory actions, either alone or in combination.
['Marinovic|M P|MP|', 'Morandi|A C|AC|', 'Otton|R|R|']
D000328:Adult D002374:Catalase D002392:Catechin D002465:Cell Movement D002470:Cell Survival D002478:Cells, Cultured D016207:Cytokines D005260:Female D005954:Glucosephosphate Dehydrogenase D005979:Glutathione Peroxidase D005980:Glutathione Reductase D006801:Humans D006997:Hypochlorous Acid D008297:Male D016328:NF-kappa B D009504:Neutrophils D009569:Nitric Oxide D009195:Peroxidase D010587:Phagocytosis D017382:Reactive Oxygen Species D015398:Signal Transduction D013482:Superoxide Dismutase D013662:Tea D051197:Toll-Like Receptor 4 D055815:Young Adult
Antioxidant Anti-inflammatory Flavonoids Inflammation Leukocyte Polyphenols