Microfluidic high performance liquid chromatography-chip hyphenation to inductively coupled plasma-mass spectrometry.
The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300nLmin-1. The potential of the instrument to eliminate common nanoLC - ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r2 of 0.9999, the peak area repeatability was 1.9% (n=7), and the detection limit was 14ngmL-1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.
['Bishop|David P|DP|', 'Blanes|Lucas|L|', 'Wilson|Alexander B|AB|', 'Wilbanks|Thor|T|', 'Killeen|Kevin|K|', 'Grimm|Rudolf|R|', 'Wenzel|Ross|R|', 'Major|Derek|D|', 'Macka|Mirek|M|', 'Clarke|David|D|', 'Schmid|Robin|R|', 'Cole|Nerida|N|', 'Doble|Philip A|PA|']
D000818:Animals D002851:Chromatography, High Pressure Liquid D006736:Horses D057230:Limit of Detection D013058:Mass Spectrometry D044085:Microfluidics D014805:Vitamin B 12
Microfluidics Chip HPLC Cobalamin NanoLC-ICP–MS