Polyacrylamide-based biocompatible Nanoplatform enhances the tumor uptake, PET/fluorescence imaging and anticancer activity of a chlorophyll analog.
In this report we demonstrate the outstanding advantages of multifunctional nanoplatforms for cancer-imaging and therapy. The non-toxic polyacrylamide (PAA) nanoparticles (size:18-25 nm) formulation drastically changed the pharmacokinetic profile of the ¹²⁴I- labeled chlorophyll-a derivative (formulated in 10% ethanol in PBS) with a remarkable enhancement in tumor uptake, and significantly reduced uptake in spleen and liver. Among the various nanoformulations investigated, the ¹²⁴I- labeled photosensitizer (dose: 0.6142 MBq), and the cyanine dye-nanoparticles (CD-NP) conjugate (dose 0.3 μmol/kg) in combination showed great potential for tumor imaging (PET/NIR fluorescence) in BALB/c mice bearing Colon26 tumors. Compared to free non-labeled photosensitizer, the corresponding PAA nanoformulation under similar treatment parameters showed a remarkable enhancement in long-term tumor cure by PDT (photodynamic therapy) and provides an opportunity to develop a single nanoplatform for tumor-imaging (PET/fluorescence) and phototherapy, a practical "See and Treat" approach.
['Gupta|Anurag|A|', 'Wang|Shouyan|S|', 'Marko|Aimee|A|', 'Joshi|Penny|P|', 'Ethirajan|Manivannan|M|', 'Chen|Yihui|Y|', 'Yao|Rutao|R|', 'Sajjad|Munawwar|M|', 'Kopelman|Raoul|R|', 'Pandey|Ravindra K|RK|']
D000818:Animals D002734:Chlorophyll D003110:Colonic Neoplasms D007457:Iodine Radioisotopes D051379:Mice D008807:Mice, Inbred BALB C D053758:Nanoparticles D010778:Photochemotherapy D017319:Photosensitizing Agents D049268:Positron-Emission Tomography D041622:Tomography, Optical
Nanoparticles photodynamic therapy Positron Imaging Tomography Reactive Oxygen species.