GRP78 is required for cell proliferation and protection from apoptosis in chicken embryo fibroblast cells.
Chicken serum has been suggested as a supplement to promote chicken cell proliferation and development. However, the molecular mechanisms by which chicken serum stimulates chicken cell proliferation remain unknown. Here, we evaluated the effects of chicken serum supplementation on chicken embryo fibroblast (CEF) and DF-1 cell proliferation. We also sought to elucidate the molecular pathways involved in mediating the effects of chicken serum on fibroblasts and DF-1 cells by overexpression of chicken 78 kDa glucose-regulated protein (chGRP78), which is important for cell growth and the prevention of apoptosis. Our data demonstrated that the addition of 5% chicken serum significantly enhanced fibroblast proliferation. Moreover, knockdown of chGRP78 using siRNA decreased fibroblast proliferation and increased apoptosis. Based on these results, we suggest that the chGRP78-mediated signaling pathway plays a critical role in chicken serum-stimulated fibroblast survival and anti-apoptosis. Therefore, our findings have important implications for the maintenance of chicken fibroblast cells through the inhibition of apoptosis and may lead to the development of new treatments for avian disease.
['Jeon|M|M|', 'Choi|H|H|', 'Lee|S I|SI|', 'Kim|J S|JS|', 'Park|M|M|', 'Kim|K|K|', 'Lee|S|S|', 'Byun|S J|SJ|']
D000818:Animals D017209:Apoptosis D049109:Cell Proliferation D002478:Cells, Cultured D002642:Chick Embryo D000091342:Endoplasmic Reticulum Chaperone BiP D005347:Fibroblasts D005786:Gene Expression Regulation D055785:Gene Knockdown Techniques D006360:Heat-Shock Proteins D034622:RNA Interference D034741:RNA, Small Interfering
cell proliferation chicken embryo fibroblast cells anti-apoptosis chicken 78kDa glucose regulated protein