Genetic polymorphism ARG753GLN of TLR-2, LEU412PHE of TLR-3, ASP299GLY of TLR-4 in patients with influenza and influenza-associated pneumonia.
OBJECTIVE
The aim of the research is to study the prevalence and to determine the prognostic significance of polymorphism ARG753GLN of the TLR-2 gene, Leu412Phe of TLR-3, Asp299Gly of TLR-4 in influenza.


PATIENTS AND METHODS
Materials and methods: 112 patients with influenza were examined (63 patients with uncomplicated course and 49 with influenza-associated pneumonia). The genotyping of the polymorphic site of ARG753GLN of the TLR-2 gene, Asp299Gly of the TLR-4 gene, and Leu412Phe of the TLR-3 gene was carried out by polymerase chain reaction using oligonucleotide primers.


RESULTS
Results: It has found that the prevalence of the mutant allele 299Gly of TLR-4 in patients with uncomplicated influenza is 6.4 %, with influenza- associated pneumonia - 7.1 %, which exceeds the population control indicators by 3.8-4.3 times (1.7 %, p<0.05). Mutant allele 412Phe of TLR-3 is significantly more common in patients with influenzaassociated pneumonia (42.9%), as compared with uncomplicated influenza (24.6%, p<0.01) and healthy people (30.0%, p<0.05). The increased risk of influenza development is associated with the Asp/Gly genotype of TLR-4 (OR=4.22) and combination of mutant genotypes Leu/Phe and Phe/Phe of TLR-3 with Asp/Gly of TLR-4 and Arg/Gln of TLR-2 (OR=15.0); influenza-associated pneumonia - with genotype Phe/Phe of TLR-3 (OR=4.5).


CONCLUSION
Conclusions: It has been found out that among patients with influenza and influenza-associated pneumonia, the mutant allele 299Gly of TLR-4 and combinations of polymorphisms Arg753Gln of TLR-2, Leu412Phe of TLR-3, Asp299Gly of TLR-4 are detected reliably more often. The frequency of the mutant allele 412Phe of TLR-3 is higher among patients with influenza-associated pneumonia. Markers of increased risk of influenza are 299Gly allele and genotype Asp/Gly of TLR-4 and the combination of mutant genotypes Leu/Phe and Phe/Phe of TLR-3 with Asp/Gly of TLR-4 and Arg/Gln of TLR-2; for influenza-associated pneumonia - allele 412Phe and genotype Phe/Phe of TLR-3.
['Pryimenko|Nataliia O|NO|', 'Kotelevska|Tetiana M|TM|', 'Koval|Tetiana I|TI|', 'Syzova|Liudmyla M|LM|', 'Dubynska|Halyna M|HM|', 'Kaidashev|Igor Р|IР|']
D016022:Case-Control Studies D020022:Genetic Predisposition to Disease D005838:Genotype D006801:Humans D007251:Influenza, Human D011014:Pneumonia D011110:Polymorphism, Genetic D051195:Toll-Like Receptor 2 D051196:Toll-Like Receptor 3 D051197:Toll-Like Receptor 4
influenza influenza-associated pneumonia genotype allele Arg753Gln polymorphism of the TLR-2 gene Asp299Gly polymorphism of the TLR-4 gene Leu412Phe polymorphism of the TLR-3 gene