Genetic characterization of Vibrio vulnificus strains isolated from oyster samples in Mexico.
Vibrio vulnificus strains were isolated from oysters that were collected at the main seafood market in Mexico City. Strains were characterized with regard to vvhA, vcg genotype, PFGE, multilocus sequence typing (MLST), and rtxA1. Analyses included a comparison with rtxA1 reference sequences. Environmental (vcgE) and clinical (vcgC) genotypes were isolated at nearly equal percentages. PFGE had high heterogeneity, but the strains clustered by vcgE or vcgC genotype. Select housekeeping genes for MLST and primers that were designed for rtxA1 domains divided the strains into two clusters according to the E or C genotype. Reference rtxA1 sequences and those from this study were also clustered according to genotype. These results confirm that this genetic dimorphism is not limited to vcg genotyping, as other studies have reported. Some environmental C genotype strains had high similarity to reference strains, which have been reported to be virulent, indicating a potential risk for oyster consumers in Mexico City.
['Guerrero|Abraham|A|', 'Gómez Gil Rodríguez|Bruno|B|', 'Wong-Chang|Irma|I|', 'Lizárraga-Partida|Marcial Leonardo|ML|']
D000818:Animals D001426:Bacterial Proteins D008800:Mexico D008969:Molecular Sequence Data D058885:Multilocus Sequence Typing D010125:Ostreidae D010802:Phylogeny D017422:Sequence Analysis, DNA D041261:Vibrio vulnificus
Vibrio vulnificus PFGE MLST rtxA1 oyster pathogens