Immune response and protective effect against Vibrio anguillarum induced by DNA vaccine encoding Hsp33 protein.
Heat shock proteins (HSPs) are considered as potent vaccine candidates against a wide range of bacterial diseases. In the present study, a recombinant DNA plasmid based on the expression of Hsp33 gene was constructed and intramuscularly administrated to flounder to elucidate whether it induces immune response and prevents the infection of Vibrio anguillarum in flounder model. Meanwhile, the expression of pHsp33 was analyzed in vitro and in vivo. The results revealed that pHsp33 was successfully expressed both in transfected hirame natural embryo cell lines and injected flounder muscle, suggesting the functionality of pHsp33 to express Hsp33 protein. Fish, when intramuscularly injected with pHsp33 vaccine, exhibited the production of specific antibodies, upregulation of immune related genes expression in the head kidney and increase of sIgM+, CD4-1+ and CD4-2+ lymphocytes in peripheral blood, spleen and head kidney, which indicated the activation of humoral and cellular immune responses. Moreover, pHsp33 upregulated the expression of immune related genes at the inoculation site, indicating the activation of local immune response. In addition, pHsp33 vaccinated flounder provided a relative percent survival of 42.86% and inhibited the pathological lesion in liver following V. anguillarum challenge. In general, the results revealed that pHsp33 could elicit local and system immune responses and confer protection for flounder, suggesting pHsp33 could serve as a DNA vaccine candidate for the control of V. anguillarum infection.
['Xu|Hongsen|H|', 'Xing|Jing|J|', 'Tang|Xiaoqian|X|', 'Sheng|Xiuzhen|X|', 'Zhan|Wenbin|W|']
D000818:Animals D000907:Antibodies, Bacterial D001426:Bacterial Proteins D002460:Cell Line D005393:Fish Diseases D005432:Flounder D005786:Gene Expression Regulation D006360:Heat-Shock Proteins D007111:Immunity, Cellular D007273:Injections, Intramuscular D007668:Kidney D010957:Plasmids D013601:T-Lymphocytes D014611:Vaccination D019444:Vaccines, DNA D014733:Vibrio D014735:Vibrio Infections
Vibrio anguillarum Local immune response System immune response Hsp33 DNA vaccine Immune protection