Chrysin-loaded PLGA attenuates OVA-induced allergic asthma by modulating TLR/NF-κB/NLRP3 axis.
Asthma, one of the significant public health problems, is triggered by certain inflammatory processes in the airways that are not addressed propitiously by current therapies. Though pieces of evidence on allergic asthma mitigation by the anti-inflammatory bioflavonoid chrysin (CHR) are accumulating, poor bioavailability, and low solubility curtail drug development. To overcome these shortcomings, CHR loaded nanoparticle (CHR-NP) was formulated, and its salutary effect in preclinical murine allergic asthma model via the peroral route was evaluated. The spherical nanosized particles showed slow, sustained release in vitro. Moreover, CHR-NP dramatically reduced the serum IgE, ovalbumin (OVA)-induced lung histological alteration, as well as Th2 (T-helper 2) cytokines in the bronchoalveolar lavage fluid (BALF). It also suppressed the elevated serum pro-inflammatory cytokines and their upstream TLR/NF-κB/NLRP3 pathway activation in lung superior to CHR and almost identical to dexamethasone (DEX). Thus this study suggests the potentiality of CHR-NP in ameliorating allergic asthma progression.
['Roy|Saheli|S|', 'Manna|Krishnendu|K|', 'Jha|Tarun|T|', 'Saha|Krishna Das|KD|']
D000072283:A549 Cells D000818:Animals D001249:Asthma D001992:Bronchoalveolar Lavage Fluid D016207:Cytokines D005419:Flavonoids D006801:Humans D006967:Hypersensitivity D007073:Immunoglobulin E D018836:Inflammation Mediators D008168:Lung D008297:Male D051379:Mice D008807:Mice, Inbred BALB C D018625:Microscopy, Atomic Force D046529:Microscopy, Electron, Transmission D016328:NF-kappa B D000071199:NLR Family, Pyrin Domain-Containing 3 Protein D010047:Ovalbumin D000077182:Polylactic Acid-Polyglycolic Acid Copolymer D051193:Toll-Like Receptors
Chrysin Murine allergic asthma model TLR/NF-κB/NLRP3 pathway Th2 cytokines