Optimal timing of apheresis for the efficient mobilization of peripheral blood progenitor cells recruited by high-dose granulocyte colony-stimulating factor in healthy donors.
Predictors of peripheral blood stem cell (PBSC) yield can potentially improve the comfort, safety, and efficacy of CD34+ cell collection from donors treated with recombinant human granulocyte colony-stimulating factor (G-CSF). We investigated 181 apheresis procedures on 109 healthy allogeneic donors to identify factors correlating with efficient PBSC collection. Apheresis started on Day 4 or 5 and continued up to Day 6 of G-CSF administration. CD34+ cell　yields on Days 4 and 5 were comparable, and significantly higher than on Day 6. This suggests that starting apheresis on Day 4 rather than Day 5 may be preferable, to reduce G-CSF exposure and optimize yield, even if multi-day collection is required. More CD34+ cells were collected from male and cytomegalovirus (CMV)-seronegative donors than from female and CMV-seropositive donors, respectively. The yields of CD34+ cells were similarly high in both male and female donors aged 20-29 years; yields decreased in female donors in their thirties, and were comparably low in both male and female donors in their forties and thereafter. These findings should guide decision-making about when to begin apheresis, and encourage careful consideration of donor factors such as gender, age, and CMV serostatus when collecting PBSCs.
['Kimura|Satoshi|S|', 'Ohkawara|Hiroshi|H|', 'Minakawa|Keiji|K|', 'Fukatsu|Masahiko|M|', 'Mori|Hirotaka|H|', 'Takahashi|Hiroshi|H|', 'Harada-Shirado|Kayo|K|', 'Ohara|Yoshihiro|Y|', 'Takahashi|Nobuhisa|N|', 'Mochizuki|Kazuhiro|K|', 'Sano|Hideki|H|', 'Nollet|Kenenth E|KE|', 'Ogawa|Kazuei|K|', 'Ohto|Hitoshi|H|', 'Kikuta|Atsushi|A|', 'Ikeda|Kazuhiko|K|', 'Ikezoe|Takayuki|T|']
D000328:Adult D001781:Blood Component Removal D001782:Blood Donors D005260:Female D016179:Granulocyte Colony-Stimulating Factor D006801:Humans D008297:Male D000072916:Peripheral Blood Stem Cells D055815:Young Adult
Apheresis Peripheral blood stem cells Cytomegalovirus Poor mobilization