Characterization of the C-terminal sequence of NS5A necessary for the assembly and production of classical swine fever virus infectious particles.
Recent studies show that classical swine fever virus (CSFV) NS5A is an essential replicase component, but it is not known how NS5A participates in viral particle production. In this study, deletion and substitution mutations were introduced into the C-terminus of CSFV NS5A. The efficiency of Core protein release and extracellular and intracellular infectivity levels were assessed and NS5A-Core interaction was investigated. These results suggested that CSFV NS5A was a key factor for the assembly of infectious CSFV particles. The C-terminal sequence from amino acids 478 to 487 and amino acids S481 and T482 were necessary for CSFV assembly and production. The effect of NS5A on CSFV assembly and production might be related to NS5A-Core interaction. T482 was found to be conserved in the C-terminus of NS5A proteins of pestiviruses and hepatitis C virus (HCV), therefore suggesting that it might be important for these virus assembly and production.
['Sheng|Chun|C|', 'Kou|Shumeng|S|', 'Jiang|Qiuyue|Q|', 'Zhou|Chenhao|C|', 'Xiao|Jing|J|', 'Li|Jing|J|', 'Chen|Bing|B|', 'Zhao|Yu|Y|', 'Wang|Yujing|Y|', 'Xiao|Ming|M|']
D000595:Amino Acid Sequence D000818:Animals D001483:Base Sequence D002460:Cell Line D002478:Cells, Cultured D006692:Classical Swine Fever Virus D004279:DNA, Viral D020043:Genes, Essential D066298:In Vitro Techniques D007668:Kidney D008969:Molecular Sequence Data D014176:Protein Biosynthesis D012324:RNA-Dependent RNA Polymerase D013552:Swine D014162:Transfection D014758:Viral Core Proteins D017361:Viral Nonstructural Proteins D014771:Virion D019065:Virus Assembly
NS5A Classical swine fever virus Core NS5A–Core interaction