Nucleic acid quantification using nicking-displacement, rolling circle amplification and bio-bar-code mediated triple-amplification.
In the present study, an inductively-coupled plasma-mass spectrometry (ICP-MS)-based triple-amplification system, by combination of nicking-displacement, rolling circle amplification (RCA) and bio-bar-code probes, was fabricated for the detection of DNA target. By using this system, hepatitis B virus (HBV) DNA target down to 3.2×10(-17)M was detected by DNA probes labeled with Au nanoparticles (AuNPs). Single nucleotide polymorphisms in genes can also be effectively discriminated. In addition, we proved that this strategy is capable of detecting the target in complicated biological samples and holds great potential application in biomedical research.
['Li|Xue-Mei|XM|', 'Luo|Jie|J|', 'Zhang|Ning-Bo|NB|', 'Wei|Qing-Li|QL|']
D015342:DNA Probes D004279:DNA, Viral D006046:Gold D006515:Hepatitis B virus D006801:Humans D053768:Metal Nanoparticles D021141:Nucleic Acid Amplification Techniques D012680:Sensitivity and Specificity D013054:Spectrophotometry, Atomic
Rolling circle amplification Bio-bar-code Triple-amplification Inductively-coupled plasma-mass spectrometry Hepatitis B virus