Human papillomavirus detection and genotyping, by HC2, full-spectrum HPV and molecular beacon real-time HPV assay in an Irish colposcopy clinic.
Cervical screening programmes are moving towards HPV testing as part of the screening process and as a triage for colposcopy. Three HPV detection methods were evaluated using cervical cytology specimens from colposcopy patients. PreservCyt™ liquid based cytology specimens from 241 women attending colposcopy clinics with greater than 2 persistently abnormal smears were recruited through the Coombe Women and Infants University Hospital, Dublin. HPV DNA was detected by Hybrid Capture (HC2) for 13 high-risk HPV types, Full-Spectrum HPV (FS-HPV) for 49 high and low-risk types and Molecular Beacon Real-Time HPV assay (MBRT-HPV) for 16 high and low-risk types. HPV genotyping was performed using Linear Array HPV Assay (LA-HPV). HPV was detected in 83.3% (195/234), 91.9% (217/236) and 80.1% (169/211) of cytology specimens by HC2, FS-HPV and MBRT-HPV, HPV DNA detection assays. The sensitivity of the assays for the detection of high-risk HPV in cytology specimens that had a Cervical Intraepithelial Neoplasia Grade 2+ result by histology were, 98%, 97% and 94% for HC2, FS-HPV and MBRT-HPV assays with positive predictive values of 94.1%, 94.1% and 97.3%. The most common HPV genotypes were HPV 16, 31, 33, 58, 42, 61 and 53, and the most common high-risk HPV genotypes were HPV 16, 31, 33, 58, 18, 45, 59, 51, 56 and 39, with detection of multiple infections in 57.7% of all cases. FS-HPV and MBRT-HPV are highly sensitive and have a similarly high PPV as the HC2 assay for detection of HPV in patients with Cervical Intraepithelial Neoplasia Grade 2+ disease. HPV genotyping of women with persistent abnormalities is warranted prior to the introduction of HPV DNA testing in a colposcopy setting.
['Keegan|Helen|H|', 'Pilkington|Loretto|L|', 'McInerney|Jamie|J|', 'Jeney|Csaba|C|', 'Benczik|Márta|M|', 'Cleary|Sinead|S|', 'von Bunau|Gunther|G|', 'Turner|Michael|M|', "D'Arcy|Tom|T|", "O' Toole|Sharon|S|", 'Pal-Szenthe|Borbála|B|', 'Kaltenecker|Borbàla|B|', 'Mózes|Johanna|J|', 'Kovács|Anette|A|', 'Solt|Agnes|A|', 'Bolger|Noel|N|', "O'Leary|John|J|", 'Martin|Cara|C|']
D000328:Adult D060085:Coinfection D005260:Female D005838:Genotype D060005:Genotyping Techniques D006801:Humans D007494:Ireland D008875:Middle Aged D025202:Molecular Diagnostic Techniques D027383:Papillomaviridae D030361:Papillomavirus Infections D011237:Predictive Value of Tests D012680:Sensitivity and Specificity D013997:Time Factors D055815:Young Adult
Genotyping HC2 HPV Irish Colposcopy GenoID