A comprehensive review on histone-mediated transfection for gene therapy.
Histone has been considered to be an effective carrier in non-viral gene delivery due to its unique properties such as efficient DNA binding ability, direct translocation to cytoplasm and favorable nuclear localization ability. Meanwhile, the rapid development of genetic engineering techniques could facilitate the construction of multifunctional fusion proteins based on histone molecules to further improve the transfection efficiency. Remarkably, histone has been demonstrated to achieve gene transfection in a synergistic manner with cationic polymers, affording to a significant improvement of transfection efficiency. In the review, we highlighted the recent developments and future trends in gene delivery mediated by histones or histone-based fusion proteins/peptides. This review also discussed the mechanism of histone-mediated gene transfection and provided an outlook for future therapeutic opportunities in the viewpoint of transfection efficacy and biosafety.
['Han|Haobo|H|', 'Yang|Jiebing|J|', 'Chen|Wenqi|W|', 'Li|Qing|Q|', 'Yang|Yan|Y|', 'Li|Quanshun|Q|']
D018014:Gene Transfer Techniques D015316:Genetic Therapy D005822:Genetic Vectors D006657:Histones D006801:Humans D010455:Peptides D014162:Transfection
Transfection Gene therapy Histone Fusion protein Synergistic effects