Neuroprotective effects of resveratrol in an MPTP mouse model of Parkinson's-like disease: possible role of SOCS-1 in reducing pro-inflammatory responses.
In the present study we used a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD) mouse model to analyze resveratrol neuroprotective effects. The MPTP-induced PD model is characterized by chronic inflammation, oxidative stress and loss of the dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc). We observed that resveratrol treatment significantly reduced glial activation, decreasing the levels of IL-1β, IL-6 and TNF-α, as well as their respective receptors in the SNpc of MPTP-treated mice, as demonstrated by Western blotting, RT-PCR and quantitative PCR analysis. This reduction is related to possible neuroprotection as we also observed that resveratrol administration limited the decline of tyrosine hydroxylase-immunoreactivity induced in the striatum and SNpc by MPTP injection. Consistent with these data, resveratrol treatment up-regulated the expression of the suppressor of cytokine signaling-1 (SOCS-1), supporting the hypothesis that resveratrol protects DA neurons of the SNpc against MPTP-induced cell loss by regulating inflammatory reactions, possibly through SOCS-1 induction.
['Lofrumento|Dario D|DD|', 'Nicolardi|Giuseppe|G|', 'Cianciulli|Antonia|A|', 'De Nuccio|Francesco|F|', 'La Pesa|Velia|V|', 'Carofiglio|Vito|V|', 'Dragone|Teresa|T|', 'Calvello|Rosa|R|', 'Panaro|Maria A|MA|']
D000818:Animals D016207:Cytokines D059290:Dopaminergic Neurons D007150:Immunohistochemistry D007249:Inflammation D020267:MPTP Poisoning D008262:Macrophage Activation D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D017072:Neostriatum D009457:Neuroglia D018696:Neuroprotective Agents D060888:Real-Time Polymerase Chain Reaction D000077185:Resveratrol D013267:Stilbenes D013378:Substantia Nigra D000071222:Suppressor of Cytokine Signaling 1 Protein D050826:Suppressor of Cytokine Signaling Proteins D014446:Tyrosine 3-Monooxygenase D015854:Up-Regulation
resveratrol SOCS-1 cytokine Parkinson’s disease dopaminergic neuron