Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique.
Super-resolution microscopy has become an increasingly popular and robust tool across the life sciences to study minute cellular structures and processes. However, with the increasing number of available super-resolution techniques has come an increased complexity and burden of choice in planning imaging experiments. Choosing the right super-resolution technique to answer a given biological question is vital for understanding and interpreting biological relevance. This is an often-neglected and complex task that should take into account well-defined criteria (e.g., sample type, structure size, imaging requirements). Trade-offs in different imaging capabilities are inevitable; thus, many researchers still find it challenging to select the most suitable technique that will best answer their biological question. This review aims to provide an overview and clarify the concepts underlying the most commonly available super-resolution techniques as well as guide researchers through all aspects that should be considered before opting for a given technique.
['Valli|Jessica|J|', 'Garcia-Burgos|Adrian|A|', 'Rooney|Liam M|LM|', 'Vale de Melo E Oliveira|Beatriz|B|', 'Duncan|Rory R|RR|', 'Rickman|Colin|C|']
D000818:Animals D002470:Cell Survival D005456:Fluorescent Dyes D006801:Humans D008853:Microscopy D056004:Molecular Dynamics Simulation
microscopy imaging diffraction limit fluorescence localization molecular dynamics molecular imaging protein–protein interactions super resolution