Functional Identification of Allograft Inflammatory Factor 1-Like Gene in Luning Chicken.
Allograft inflammatory factor-1 (AIF-1) is an inflammation-related protein mainly produced by immune cells, such as monocyte/macrophages and activated T lymphocytes. It is essential for the survival and proinflammatory activity of immune cells. However, the function of AIF-1 in chicken still has not been defined. In the present study, AIF-1-like (AIF1L) gene was identified in Luning chicken. Bioinformatics analysis revealed that the molecular weight of the chicken AIF-1 protein was 16290.8 Da. AIF1L contained a Ca2+ binding EF hand and could interact with actin filament. Its transcript was found in all tested tissues including spleen, brain, heart, kidney, liver, thymus, bursa of Fabricius, lung, and a relative low-level expression was detected in leg muscle. Furthermore, AIF1L expression in peripheral blood lymphocyte was depressed in a dose-dependent manner with cadmium exposure and peripheral blood lymphocyte viability decrease displayed a similar pattern with AIF1L expression. The results indicated that newly identified chicken AIF1L might be associated with lymphocyte viability.
['Zhao|Yan-Ying|YY|', 'Lin|Ya-Qiu|YQ|', 'Xu|Ya-Ou|YO|']
D000818:Animals D030161:Avian Proteins D002104:Cadmium D002135:Calcium-Binding Proteins D002470:Cell Survival D002645:Chickens D015870:Gene Expression D008214:Lymphocytes D008840:Microfilament Proteins D060888:Real-Time Polymerase Chain Reaction D018675:Toxicity Tests
Luning chicken Allograft inflammatory factor-1 lymphocyte cadmium