Effects of prebiotic inulin-type fructans on blood metabolite and hormone concentrations and faecal microbiota and metabolites in overweight dogs.
Because obesity is associated with many co-morbidities, including diabetes mellitus, this study evaluated the second-meal effect of a commercial prebiotic, inulin-type fructans, and the effects of the prebiotic on faecal microbiota, metabolites and bile acids (BA). Nine overweight beagles were used in a replicated 3×3 Latin square design to test a non-prebiotic control (cellulose) against a low (equivalent to 0·5 % diet) and high dose (equivalent to 1·0 % diet) of prebiotic over 14-d treatments. All dogs were fed the same diet twice daily, with treatments provided orally via gelatin capsules before meals. On days 13 or 14 of each period, fresh faecal samples were collected, dogs were fed at 08.00 hours and then challenged with 1 g/kg body weight of maltodextrin in place of the 16.00 hours meal. Repeated blood samples were analysed for glucose and hormone concentrations to determine postprandial incremental AUC (IAUC) data. Baseline glucose, insulin and active glucagon-like peptide-1 levels were similar between all groups (P>0·10). Glucose and insulin IAUC after glucose challenge appeared lower following the high dose, but did not reach statistical relevance. Prebiotic intervention resulted in an increase in relative abundance of some Firmicutes and a decrease in the relative abundance of some Proteobacteria. Individual and total faecal SCFA were significantly increased (P<0·05) following prebiotic supplementation. Total concentration of excreted faecal BA tended to increase in dogs fed the prebiotic (P=0·06). Our results indicate that higher doses of inulin-type prebiotics may serve as modulators of gut microbiota, metabolites and BA pool in overweight dogs.
['Alexander|Celeste|C|0000-0001-8162-7590', 'Cross|Tzu-Wen L|TL|', 'Devendran|Saravanan|S|', 'Neumer|Franka|F|', 'Theis|Stephan|S|', 'Ridlon|Jason M|JM|', 'Suchodolski|Jan S|JS|', 'de Godoy|Maria R C|MRC|', 'Swanson|Kelly S|KS|']
D000818:Animals D019540:Area Under Curve D001647:Bile Acids and Salts D001786:Blood Glucose D003106:Colon D004285:Dogs D005232:Fatty Acids, Volatile D005243:Feces D005260:Female D000068536:Firmicutes D005630:Fructans D000069196:Gastrointestinal Microbiome D052216:Glucagon-Like Peptide 1 D007328:Insulin D007444:Inulin D009765:Obesity D019518:Postprandial Period D056692:Prebiotics D020560:Proteobacteria
Second-meal effect Bile acids SCFA Canine nutrition