Structure Engineering of a Lanthanide-Based Metal-Organic Framework for the Regulation of Dynamic Ranges and Sensitivities for Pheochromocytoma Diagnosis.
Exploring innovative technologies to precisely quantify biomolecules is crucial but remains a great challenge for disease diagnosis. Unfortunately, the humoral concentrations of most biotargets generally vary within rather limited scopes between normal and pathological states, while most literature-reported biosensors can detect large spans of targets concentrations, but are less sensitive to small concentration changes, which consequently make them mostly unsatisfactory or even unreliable in distinguishing positives from negatives. Herein, a novel strategy of precisely quantifying the small concentration changes of a certain biotarget by editing the dynamic ranges and sensitivities of a lanthanide-based metal-organic framework (Eu-ZnMOF) biosensor is reported. By elaborately tailoring the biosensor's structure and surface areas, the tunable Eu-ZnMOF is developed with remarkably enhanced response slope within the "optimized useful detection window," enabling it to serve as a powerful signal amplifier (87.2-fold increase) for discriminating the small concentration variation of urinary vanillylmandelic acid (an early pathological signature of pheochromocytoma) within only three times between healthy and diseased subjects. This study provides a facile approach to edit the biosensors' performances through structure engineering, and exhibits promising perspectives for future clinical application in the non-invasive and accurate diagnosis of severe diseases.
['Hao|Ji-Na|JN|', 'Niu|Dechao|D|', 'Gu|Jinlou|J|', 'Lin|Shaoliang|S|', 'Li|Yongsheng|Y|https://orcid.org/0000-0001-5621-9528', 'Shi|Jianlin|J|']
D000310:Adrenal Gland Neoplasms D000818:Animals D015374:Biosensing Techniques D045744:Cell Line, Tumor D004738:Engineering D005063:Europium D006801:Humans D000073396:Metal-Organic Frameworks D051379:Mice D010673:Pheochromocytoma D014642:Vanilmandelic Acid
metal-organic frameworks pheochromocytoma diagnosis vanillylmandelic acid tailorable structures tunable biosensors