Allicin functionalized locust bean gum nanoparticles for improved therapeutic efficacy: An in silico, in vitro and in vivo approach.
The field of nanotechnology has overgrown over the past few years and has even ventured into the field of medicine. The aim of the present study is to develop a novel allicin functionalized locust bean gum nanoparticle using the nanoprecipitation technique. The synthesized nanoparticles were characterized by dynamic light scattering, scanning electron microscopy and transmission electron microscopy. The characterization study revealed the nanoscale structure (∼100nm) of the prepared particles. In silico toxicology analysis were carried out to assess the drug-like properties and virtual toxicity of allicin. Toxicity of the prepared nanoparticles were carried out in RAW 264.7 cell lines in vitro and in vivo studies were carried out in Sprague-Dawley rats. In in vitro study, LBGAN showed a maximum toxicity of 10.51% in MTT assay, no reactive oxygen species generation on DCFDA staining and LBGAN was effective to protect the cells from apoptosis. In in vivo toxicity studies LBGAN showed no significant change in the activities of the marker enzymes like LDH, CK-MB, ALP, ACP, AST and ALT. Thus, the functionalization of nanoparticles with allicin has the benefit of providing protection and stability to the allicin, in addition to increasing its pharmacological activity.
['Soumya|R S|RS|', 'Sherin|S|S|', 'Raghu|K G|KG|', 'Abraham|Annie|A|']
D000818:Animals D017209:Apoptosis D015415:Biomarkers D002470:Cell Survival D004220:Disulfides D004337:Drug Carriers D016503:Drug Delivery Systems D065546:Drug Liberation D005685:Galactans D008111:Liver Function Tests D008351:Mannans D051379:Mice D053758:Nanoparticles D064209:Phytochemicals D053149:Plant Gums D000067996:RAW 264.7 Cells D051381:Rats D013441:Sulfinic Acids
Allicin Locust bean gum Therapeutics Nanoprecipitation Drug delivery In silico analysis