Combining Fluorescence and Bioluminescence Microscopy to Study the Series of Events from Cellular Signal Transduction to Gene Expression.
The molecular interactions and translocation of signal transduction factors in individual cells can be imaged by fluorescence microscopy. Alternatively, downstream promoter activity in single cells can be imaged by bioluminescence microscopy. However, the same stimuli can lead to different gene expression responses in individual cells. For this reason, it is desirable to simultaneously image signal transduction and gene expression events in the same cells. Here, we describe a method that combines fluorescence and bioluminescence microscopy to image protein kinase C (PKC) translocation from the cytosol to the plasma membrane and the expression of nuclear factor kappa-light polypeptide B (NF-κB)-regulated genes. © 2017 by John Wiley & Sons, Inc.
['Goda|Kazuhito|K|', 'Takahashi|Takeo|T|', 'Suzuki|Hirobumi|H|']
D004867:Equipment Design D015870:Gene Expression D005786:Gene Expression Regulation D006367:HeLa Cells D006801:Humans D049449:Luminescence D008856:Microscopy, Fluorescence D016328:NF-kappa B D061848:Optical Imaging D011493:Protein Kinase C D021381:Protein Transport D015398:Signal Transduction D059010:Single-Cell Analysis
fluorescence bioluminescence signal transduction gene expression imaging single-cell analysis