Profiling Novel Alternative Splicing within Multiple Tissues Provides Useful Insights into Porcine Genome Annotation.
Alternative splicing (AS) is a process during gene expression that results in a single gene coding for different protein variants. AS contributes to transcriptome and proteome diversity. In order to characterize AS in pigs, genome-wide transcripts and AS events were detected using RNA sequencing of 34 different tissues in Duroc pigs. In total, 138,403 AS events and 29,270 expressed genes were identified. An alternative donor site was the most common AS form and accounted for 44% of the total AS events. The percentage of the other three AS forms (exon skipping, alternative acceptor site, and intron retention) was approximately 19%. The results showed that the most common AS events involving alternative donor sites could produce different transcripts or proteins that affect the biological processes. The expression of genes with tissue-specific AS events showed that gene functions were consistent with tissue functions. AS increased proteome diversity and resulted in novel proteins that gained or lost important functional domains. In summary, these findings extend porcine genome annotation and highlight roles that AS could play in determining tissue identity.
['Feng|Wen|W|', 'Zhao|Pengju|P|', 'Zheng|Xianrui|X|', 'Hu|Zhengzheng|Z|', 'Liu|Jianfeng|J|']
D017398:Alternative Splicing D000818:Animals D005091:Exons D020869:Gene Expression Profiling D016678:Genome D006801:Humans D007438:Introns D058977:Molecular Sequence Annotation D020543:Proteome D017423:Sequence Analysis, RNA D013552:Swine D059467:Transcriptome
alternative splicing protein transcript domain single nucleotide polymorphism