Transfection of Sox11 plasmid alleviates ventilator-induced lung injury via Sox11 and FAK.
Background Ventilator-induced lung injury (VILI) is the most common complication in the mechanical ventilation in clinic. The pathogenesis of VILI has not been well understood. The SRY related High Mobility Group box group-F family member 11(Sox11) is a protein associated with lung development. The focal adhesion kinase(FAK) is a cytoplasmic tyrosine kinase and is regulated by Sox11. The present study, therefore, was undertaken to explore the potential role of Sox11 and FAK in VILI. Methods High volume mechanical ventilation(HMV) was used to establish mouse VILI model under anesthesia. The lung injury was evaluated by analyzing the lung weight, bronchoalveolar lavage fluid, histopathological changes and apoptosis of the lung. The Sox11 and FAK expressions in the lung were investigated by real-time qPCR, western blot and immunohistochemistry analysis. Results HMV induced VILI simultaneously companied with decreased expressions of Sox11 and FAK in alveolar epithelial and interstitial cells either in gene and protein levels. Transfection of Sox11 plasmid significantly upregulated expressions of Sox11 and FAK in gene and protein levels in the lung and particularly effectively alleviated VILI. Furthermore, FAK antagonism by PF562271(FAK antagonist) blocked the alleviating effect of Sox11 plasmid transfection on the VILI. Conclusion The dysregulation in the Sox11 and FAK after HMV play an important role in the pathogenesis of VILI, and facilitating the activity of Sox11and FAK might be an effective target and potential option in the prevention and treatment of VILI in clinic.
['Fang|Mingxing|M|', 'Fan|Shujuan|S|', 'Yao|Xiaoguang|X|', 'Liu|Na|N|', 'Gao|Junxia|J|', 'Wang|Zhiyong|Z|', 'Xu|Tieling|T|', 'Xian|Xiaohui|X|', 'Li|Wenbin|W|']
D000818:Animals D004195:Disease Models, Animal D015536:Down-Regulation D051417:Focal Adhesion Kinase 1 D015316:Genetic Therapy D008297:Male D008810:Mice, Inbred C57BL D010957:Plasmids D055751:SOXC Transcription Factors D014162:Transfection D015854:Up-Regulation D055397:Ventilator-Induced Lung Injury
Sox11 FAK Mouse Ventilator induced lung injury