Enzymatic degradation and bioactivity evaluation of C-6 oxidized chitosan.
C-6 oxidized chitosan was produced from chitosan by performing selective oxidation with NaOCl and NaBr using 2,2,6,6-tetramethylpiperidine-1-oxy radical (TEMPO) as catalyst. Endocellulase, Celluclast 1.5 L, Glucanex(®), Macerozyme R-10, hyaluronidase, hyaluronate lyase, red scorpionfish chitinase, glucuronan lyase and a protein mix from Trichoderma reesei were used to degrade the C-6 oxidized chitosan. Glucanex(®), the crude extract from T. reesei IHEM 4122 and Macerozyme R-10 validated the enzymatic degradation through final hydrolysis yields of the derivative respectively close to 36.4, 20.3 and 12.9% (w/w). The best initial reaction velocity (2.41 U/mL) was observed for Glucanex(®). The antileishmanial activity of the derivative was evaluated against Leishmania infantum LIPA 137. The antibacterial activities against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were also tested. Results showed an antileishmanial activity (IC50: 125 μg/mL) of the obtained derivatives against L. infantum LIPA 137.
['Pierre|Guillaume|G|', 'Salah|Rym|R|', 'Gardarin|Christine|C|', 'Traikia|Mounir|M|', 'Petit|Emmanuel|E|', 'Delort|Anne-Marie|AM|', 'Mameri|Nabil|N|', 'Moulti-Mati|Farida|F|', 'Michaud|Philippe|P|']
D000900:Anti-Bacterial Agents D000981:Antiprotozoal Agents D048271:Chitosan D003497:Cyclic N-Oxides D006868:Hydrolysis D010084:Oxidation-Reduction
Chitosan Leishmania TEMPO oxidation