Non-specific defensive factors of the Pacific oyster Crassostrea gigas against infection with Marteilioides chungmuensis: a flow-cytometric study.
In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas.
['Choi|Hee Jung|HJ|', 'Hwang|Jee Youn|JY|', 'Choi|Dong Lim|DL|', 'Huh|Min Do|MD|', 'Hur|Young Baek|YB|', 'Lee|Nam-Sil|NS|', 'Seo|Jung Soo|JS|', 'Kwon|Mun Gyeong|MG|', 'Choi|Hye-Sung|HS|', 'Park|Myoung Ae|MA|']
D000818:Animals D002452:Cell Count D002470:Cell Survival D056907:Cercozoa D049896:Crassostrea D005434:Flow Cytometry D006098:Granulocytes D006434:Hemocytes D010587:Phagocytosis
pacific oyster Crassostrea gigas Marteilioides chungmuensis hemocyte flow cytometry