Cell-cultured, live attenuated, X-31ca-based H5N1 pre-pandemic influenza vaccine.
The manufacture of influenza vaccines has traditionally depended upon a method using embryonated hen's eggs. However, concerns regarding the potential shortage of the influenza substrate in the event of a pandemic has led to the development of cell culture-derived vaccines, which offers shorter lead-in times and greater production flexibility. We examined optimal conditions for the production of reassortant X-31ca-based H5N1 cold-adapted live attenuated influenza vaccine (rH5N1ca) cultured in mammalian cell lines. During ten passages in MDCK cells, the rH5N1ca vaccine maintained cold-adapted and temperature-sensitive phenotypes, and no mutations occurred in the hemagglutinin and neuraminidase surface antigens, demonstrating genetic and phenotypic stability. Single immunization in mice with the rH5N1ca induced robust antibody responses and protected the mice against lethal challenge. Stable maintenance of attenuation phenotypes and immunogenicity of the rH5N1ca from cell-culture suggest that they can be produced as a stockpile for pandemic preparedness as an alternative to current egg-based production.
['Lee|Yun Ha|YH|', 'Jang|Yo Han|YH|', 'Seong|Baik Lin|BL|']
D000818:Animals D002460:Cell Line D002522:Chlorocebus aethiops D004285:Dogs D005260:Female D019267:Hemagglutinin Glycoproteins, Influenza Virus D053124:Influenza A Virus, H5N1 Subtype D007252:Influenza Vaccines D061985:Madin Darby Canine Kidney Cells D051379:Mice D008807:Mice, Inbred BALB C D009439:Neuraminidase D009976:Orthomyxoviridae Infections D058873:Pandemics D064166:Vaccine Potency D014613:Vaccines, Attenuated D014709:Vero Cells
Cell culture HPAI H5N1 Influenza virus Live vaccine