GroE chaperonins assisted functional expression of bacterial enzymes in Saccharomyces cerevisiae.
Rapid advances in the capabilities of reading and writing DNA along with increasing understanding of microbial metabolism at the systems-level have paved an incredible path for metabolic engineering. Despite these advances, post-translational tools facilitating functional expression of heterologous enzymes in model hosts have not been developed well. Some bacterial enzymes, such as Escherichia coli xylose isomerase (XI) and arabinose isomerase (AI) which are essential for utilizing cellulosic sugars, cannot be functionally expressed in Saccharomyces cerevisiae. We hypothesized and demonstrated that the mismatching of the HSP60 chaperone systems between bacterial and eukaryotic cells might be the reason these bacterial enzymes cannot be functionally expressed in yeast. The results showed that the co-expression of E. coli GroE can facilitate the functional expression of E. coli XI and AI, as well as the Agrobacterium tumefaciens D-psicose epimerase in S. cerevisiae. The co-expression of bacterial chaperonins in S. cerevisiae is a promising post-translational strategy for the functional expression of bacterial enzymes in yeast. Biotechnol. Bioeng. 2016;113: 2149-2155. © 2016 Wiley Periodicals, Inc.
['Xia|Peng-Fei|PF|', 'Zhang|Guo-Chang|GC|', 'Liu|Jing-Jing|JJ|', 'Kwak|Suryang|S|', 'Tsai|Ching-Sung|CS|', 'Kong|In Iok|II|', 'Sung|Bong Hyun|BH|', 'Sohn|Jung-Hoon|JH|', 'Wang|Shu-Guang|SG|', 'Jin|Yong-Su|YS|']
D001426:Bacterial Proteins D018834:Chaperonin 60 D029968:Escherichia coli Proteins D015964:Gene Expression Regulation, Bacterial D015971:Gene Expression Regulation, Enzymologic D006360:Heat-Shock Proteins D060847:Metabolic Engineering D018832:Molecular Chaperones D015202:Protein Engineering D011499:Protein Processing, Post-Translational D011994:Recombinant Proteins D012441:Saccharomyces cerevisiae
GroE chaperonins Saccharomyces cerevisiae metabolic engineering post-translation Escherichia coli xylose isomerase