HILIC-ESI-FTMS with All Ion Fragmentation (AIF) Scans as a Tool for Fast Lipidome Investigations.
Lipidomics suffers from the lack of fast and reproducible tools to obtain both structural information on intact phospholipids (PL) and fatty acyl chain composition. Hydrophilic interaction liquid chromatography with electrospray ionization coupled to an orbital-trap Fourier-transform analyzer operating using all ion fragmentation mode (HILIC-ESI-FTMS-AIF MS) is seemingly a valuable resource in this respect. Here, accurate m/z values, HILIC retention times and AIF MS scan data were combined for PL assignment in standard mixtures or real lipid extracts. AIF scans in both positive and negative ESI mode, achieved using collisional induced dissociation for fragmentation, were applied to identify both the head-group of each PL class and the fatty acyl chains, respectively. An advantage of the AIF approach was the concurrent collection of tandem MS-like data, enabling the identification of linked fatty acyl chains of precursor phospholipids through the corresponding carboxylate anions. To illustrate the ability of AIF in the field of lipidomics, two different types of real samples, i.e., the lipid extracts obtained from human plasma and dermal fibroblasts, were examined. Using AIF scans, a total of 253 intact lipid species and 18 fatty acids across 4 lipid classes were recognized in plasma samples, while FA C20:3 was confirmed as the fatty acyl chain belonging to phosphatidylinositol, PI 38:3, which was found to be down-regulated in fibroblast samples of Parkinson's disease patients.
['Ventura|Giovanni|G|', 'Bianco|Mariachiara|M|', 'Calvano|Cosima Damiana|CD|0000-0001-8832-7072', 'Losito|Ilario|I|0000-0003-0025-3350', 'Cataldi|Tommaso R I|TRI|0000-0003-4811-8959']
D002853:Chromatography, Liquid D005227:Fatty Acids D005347:Fibroblasts D006801:Humans D057927:Hydrophobic and Hydrophilic Interactions D000081362:Lipidomics D059625:Liquid-Liquid Extraction D010300:Parkinson Disease D061251:Primary Cell Culture D012867:Skin D021241:Spectrometry, Mass, Electrospray Ionization
phospholipids collisional induced dissociation fatty acids AIF scan MS HILIC separation