Expression pattern of CD55 and CD59 on red blood cells in sickle cell disease.
OBJECTIVES
To investigate the pattern of CD55 and CD59 expression on RBCs of SCD patients, and its association with anemia, biochemical parameters of hemolysis, level of erythropoietin, and pro-inflammatory markers.


METHODS
Flow cytometric analysis was performed on RBCs from 71 adult SCD patients and 53 healthy controls, using the commercial REDQUANT kit.


RESULTS
CD59 deficiency was significantly higher among SCD patients than among healthy controls. The proportions of CD55-deficient and CD59-deficient RBCs from SCD patients were significantly higher when compared with those from healthy controls (0.17 vs. 0.09 and 2.1 vs. 1.2, respectively). The MFI of CD55 and CD59 expression on RBCs in SCD was significantly reduced when compared to the expression in healthy controls (5.2 vs. 6.4 and 19.4 vs 20.3, respectively). The pattern of CD55 and CD59 expression was not correlated with anemia, biomarkers of hemolysis, erythropoietin level, or other pro-inflammatory markers.


DISCUSSION
There is an altered pattern of CD55 and CD59 expression on RBCs of SCD Patients; however, it does not seem to play a causal role in the pathophysiology of anemia, and is unlikely to be influenced by the level of erythropoietin or other inflammatory mediators.
['Al-Faris|Lama|L|', 'Al-Rukhayes|Monera|M|', 'Al-Humood|Salah|S|']
D000328:Adult D000755:Anemia, Sickle Cell D018958:CD55 Antigens D018957:CD59 Antigens D004912:Erythrocytes D004921:Erythropoietin D005260:Female D005434:Flow Cytometry D006801:Humans D008297:Male D055815:Young Adult
CD55 CD59 Sickle Cell Disease (SCD)