An international study comparing conventional versus mRNA level testing (TargetPrint) for ER, PR, and HER2 status of breast cancer.
To compare results from messenger RNA (mRNA)-based TargetPrint testing with those from immunohistochemistry (IHC) and in situ hybridization (ISH) conducted according to local standard procedures at hospitals worldwide. Tumor samples were prospectively obtained from 806 patients at 22 hospitals. The mRNA level of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) was assessed by TargetPrint quantitative gene expression readouts. IHC/ISH assessments were performed according to local standards at the participating hospitals. TargetPrint readout showed a high concordance with IHC/ISH of 95 % (kappa 0.81) for ER, 81 % (kappa 0.56) for PR, and 94 % (kappa 0.76) for HER2. The positive/negative agreement between TargetPrint and IHC for ER, PR, and HER2 was 96 %/87 %, 84 %/74 %, and 74 %/98 %, respectively. The concordance rate in IHC/ISH results between hospitals varied: 88-100 % for ER (kappa 0.50-1.00); 50-100 % for PR (kappa 0.20-1.00); and 90-100 % for HER2 (kappa 0.59-1.00). mRNA readout of ER, PR, and HER2 status by TargetPrint was largely comparable to local IHC/ISH analysis. However, there was substantial discordance in IHC/ISH results between different hospitals. When results are discordant, the use of TargetPrint would improve the reliability of hormone receptor and HER2 results by prompting retesting in a reference laboratory.
['Wesseling|Jelle|J|', 'Tinterri|Corrado|C|', 'Sapino|Anna|A|', 'Zanconati|Fabrizio|F|', 'Lutke-Holzik|Martijn|M|', 'Nguyen|Bichlien|B|', 'Deck|Kenneth B|KB|', 'Querzoli|Patrizia|P|', 'Perin|Tiziana|T|', 'Giardina|Carmela|C|', 'Seitz|Gerhard|G|', 'Guinebretière|Jean-Marc|JM|', 'Barone|Julie|J|', 'Dekker|Laura|L|', 'de Snoo|Femke|F|', 'Stork-Sloots|Lisette|L|', 'Roepman|Paul|P|', 'Watanabe|Toru|T|', 'Cusumano|Pino|P|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D014408:Biomarkers, Tumor D001943:Breast Neoplasms D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D007150:Immunohistochemistry D008875:Middle Aged D018719:Receptor, ErbB-2 D011960:Receptors, Estrogen D011980:Receptors, Progesterone D015203:Reproducibility of Results D055815:Young Adult
mRNA ER Breast cancer Her-2 IHC/Fish PgR