Diagnostic Capacity of RASSF1A Promoter Methylation as a Biomarker in Tissue, Brushing, and Blood Samples of Nasopharyngeal Carcinoma.
Methylation of the RAS association domain family protein 1A (RASSF1A) promoter has been observed in nasopharyngeal carcinoma (NPC). This study investigated the correlation of RASSF1A promoter methylation with clinicopathological features and its utility as a diagnostic biomarker in NPC. A total of 926 patients with NPC and 495 non-tumor controls were analyzed in this study. RASSF1A promoter methylation was notably higher in NPC compared with non-tumor tissue, brushing and blood samples. RASSF1A promoter methylation was associated with clinical stage, lymph node status, distant metastasis, and T classification of patients with NPC, although it was not linked to age and sex. The pooled sensitivity, specificity, and AUC (area under the curve) of RASSF1A promoter methylation were determined in NPC samples vs. non-tumor samples (tissue: sensitivity=0.72, specificity=0.99, AUC=0.98; brushing: sensitivity=0.56, specificity=1.00, AUC=0.94; blood: sensitivity=0.11, specificity=0.98, AUC=0.97). Our findings show that RASSF1A promoter methylation may be correlated with the development, progression and metastasis of NPC. RASSF1A promoter methylation is a promising noninvasive biomarker for the diagnosis of NPC from tissue and brushing samples.
['Ye|Meng|M|', 'Huang|Tao|T|', 'Ni|Chao|C|', 'Yang|Ping|P|', 'Chen|Si|S|']
D019540:Area Under Curve D014408:Biomarkers, Tumor D002277:Carcinoma D019175:DNA Methylation D016208:Databases, Factual D006801:Humans D008198:Lymph Nodes D000077274:Nasopharyngeal Carcinoma D009303:Nasopharyngeal Neoplasms D016017:Odds Ratio D011401:Promoter Regions, Genetic D012372:ROC Curve D025521:Tumor Suppressor Proteins
RASSF1A Methylation Biomarker NPC Diagnosis Prognosis