ITSN2L Interacts with and Negatively Regulates RABEP1.
Intersectin-2Long (ITSN2L) is a multi-domain protein participating in endocytosis and exocytosis. In this study, RABEP1 was identified as a novel ITSN2L interacting protein using a yeast two-hybrid screen from a human brain cDNA library and this interaction, specifically involving the ITSN2L CC domain and RABEP1 CC3 regions, was further confirmed by in vitro GST (glutathione-S-transferase) pull-down and in vivo co-immunoprecipitation assays. Corroboratively, we observed that these two proteins co-localize in the cytoplasm of mammalian cells. Furthermore, over-expression of ITSN2L promotes RABEP1 degradation and represses RABEP1-enhanced endosome aggregation, indicating that ITSN2L acts as a negative regulator of RABEP1. Finally, we showed that ITSN2L and RABEP1 play opposite roles in regulating endocytosis. Taken together, our results indicate that ITSN2L interacts with RABEP1 and stimulates its degradation in regulation of endocytosis.
['Yang|Xiaoxu|X|', 'Yan|Feng|F|', 'He|Zhicheng|Z|', 'Liu|Shan|S|', 'Cheng|Yeqing|Y|', 'Wei|Ke|K|', 'Gan|Shiquan|S|', 'Yuan|Jing|J|', 'Wang|Shang|S|', 'Xiao|Ye|Y|', 'Ren|Kaiqun|K|', 'Liu|Ning|N|', 'Hu|Xiang|X|', 'Ding|Xiaofeng|X|', 'Hu|Xingwang|X|', 'Xiang|Shuanglin|S|']
D033942:Adaptor Proteins, Vesicular Transport D002460:Cell Line D004705:Endocytosis D005786:Gene Expression Regulation D055785:Gene Knockdown Techniques D006801:Humans D047468:Immunoprecipitation D042541:Intracellular Space D019151:Peptide Library D011485:Protein Binding D054730:Protein Interaction Domains and Motifs D025941:Protein Interaction Mapping D021381:Protein Transport D059748:Proteolysis D020798:Two-Hybrid System Techniques D033921:Vesicular Transport Proteins
ITSN2L interactions RABEP1 endocytosis endosome