A luminescence-based probe for sensitive detection of hydrogen peroxide in seconds.
Here, we present a fast and simple hydrogen peroxide assay that is based on time-resolved fluorescence. The emission intensity of a complex consisting of terbium ions (Tb(3+)) and phthalic acid (PA) in HEPES buffer is quenched in the presence of H2O2 and this quenching is concentration-dependent. The novel PATb assay detects hydrogen peroxide at a pH range from 7.5 to 8.5 and with a detection limit of 150 nmol L(-1) at pH 8.5. The total assay time is less than 1 min. The linear range of the assay can be adapted by a pH adjustment of the aqueous buffer and covers a concentration range from 310 nmol L(-1) to 2.56 mmol L(-1) in total which encompasses four orders of magnitude. The assay is compatible with high concentrations of all 47 tested inorganic and organic compounds. The PATb assay was applied to quantify H2O2 in polluted river water samples. In conclusion, this fast and easy-to-use assay detects H2O2 with high sensitivity and precision.
['Zscharnack|Kristin|K|', 'Kreisig|Thomas|T|', 'Prasse|Agneta A|AA|', 'Zuchner|Thole|T|']
D002412:Cations D006861:Hydrogen Peroxide D057230:Limit of Detection D049449:Luminescence D049408:Luminescent Agents D008163:Luminescent Measurements D010795:Phthalic Acids D045483:Rivers D013725:Terbium D014874:Water Pollutants, Chemical
Hydrogen peroxide Phthalic acid Assay development Dynamic quenching Lanthanide Luminescence