Evaluation of a novel automated water analyzer for continuous monitoring of toxicity and chemical parameters in municipal water supply.
A novel tool, the DAMTA analyzer (Device for Analytical Monitoring and Toxicity Assessment), designed for fully automated toxicity measurements based on luminescent bacteria as well as for concomitant determination of chemical parameters, was developed and field-tested. The instrument is a robotic water analyzer equipped with a luminometer and a spectrophotometer, integrated on a thermostated reaction plate which contains a movable carousel with 80 cuvettes. Acute toxicity is measured on-line using a wild type Photobacterium phosphoreum strain with measurable bioluminescence and unaltered sensitivity to toxicants lasting up to ten days. The EC50 values of reference compounds tested were consistent with A. fischeri and P. phosphoreum international standards and comparable to previously published data. Concurrently, a laboratory trial demonstrated the feasibility of use of the analyzer for the determination of nutrients and metals in parallel to the toxicity measurements. In a prolonged test, the system was installed only in toxicity mode at the premises of the World Fair "Expo Milano-2015″, a high security site to ensure the quality of the supplied drinking water. The monitoring program lasted for six months during which ca. 2400 toxicity tests were carried out; the results indicated a mean non-toxic outcome of -5.5 ± 6.2%. In order to warrant the system's robustness in detecting toxic substances, Zn was measured daily with highly reproducible inhibition results, 70.8 ± 13.6%. These results assure that this novel toxicity monitor can be used as an early warning system for protection of drinking water sources from emergencies involving low probability/high impact contamination events in source water or treated water.
['Bodini|Sergio F|SF|', 'Malizia|Marzio|M|', 'Tortelli|Annalisa|A|', 'Sanfilippo|Luca|L|', 'Zhou|Xingpeng|X|', 'Arosio|Roberta|R|', 'Bernasconi|Marzia|M|', 'Di Lucia|Stefano|S|', 'Manenti|Angela|A|', 'Moscetta|Pompeo|P|']
D060766:Drinking Water D004784:Environmental Monitoring D004867:Equipment Design D007558:Italy D008163:Luminescent Measurements D010776:Photobacterium D012371:Robotics D018675:Toxicity Tests D014874:Water Pollutants, Chemical D014881:Water Supply
Luminescent bacteria Acute toxicity Photobacterium phosphoreum Automated analyzer On-line monitoring Water quality