A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test.
Toxoplasma gondii infection is one of the most common human zoonosis. Laboratory diagnosis of this disease is mainly based on the results of serological methods detecting specific antibodies in the patient's sera. In this study we aimed to evaluate the performance of a chemiluminescence immunoassay (CLIA) based on the use of a novel immunochemical reagent in the form of the conjugate of original acridinium label (AL) attached to secondary antibody (IgG-AL) and SAG2-GRA1-ROP1L chimeric antigen for T. gondii specific antibodies detection. The CLIA test was compared with conventional ELISA, which was based on the same recombinant antigen and differed only in terms of the detection methodology of immune complexes. The new CLIA assay proved to be more sensitive and better differentiated sera of patients with T. gondii infection from sera of healthy individuals, being a promising alternative to more labor, cost-demanding and less versatile ELISA as screening test in toxoplasmosis diagnostics.
['Holec-Gąsior|Lucyna|L|', 'Ferra|Bartłomiej|B|', 'Czechowska|Justyna|J|', 'Serdiuk|Illia E|IE|', 'Krzymiński|Karol|K|']
D000913:Antibodies, Protozoan D000953:Antigens, Protozoan D004797:Enzyme-Linked Immunosorbent Assay D005260:Female D006801:Humans D007118:Immunoassay D007074:Immunoglobulin G D008163:Luminescent Measurements D015800:Protozoan Proteins D011993:Recombinant Fusion Proteins D014122:Toxoplasma D014123:Toxoplasmosis
Chemiluminescence Acridinium esters ELISA CLIA Chimeric antigen Serological detection