Comparative genotyping of Blastocystis infecting cattle and human in the south of Iran.
BACKGROUND
Blastocystis is a unicellular protozoan and one of the most common parasites found in humans and many animals' intestinal tract. The present study aimed to compare the genotypes of Blastocystis infecting cattle and humans in the south of Iran.


METHODS
A total of 100 human stool samples and 75 cattle stool samples were microscopically examined for Blastocystis infection. DNA was extracted from thirty-eight microscopically positive samples (13 humans and 25 cattle). PCR was performed on positive samples targeting the Blastocystis-specific SSU rDNA gene. PCR products of eight humans and eleven cattle samples were sequenced and compared with available reference sequences in GenBank by BLAST queries. Genetic diversity was measured for Blastocystis subtypes in human and cattle, based on haplotype and nucleotide diversities.


RESULTS
The PCR detected Blastocystis in ten humans and twenty-four cattle samples. Blastocystis subtypes 1, 2, and 6 were found in humans whereas subtypes 5 and 10 were found in cattle. Subtype (ST) 2 was the most predominant subtypes in humans whereas, in cattle specimens, the ST5 was the most dominant subtype. Based on the Blastocystis sequences of SSU rDNA, 68 sites were polymorphic and 49 sites were parsimony informative, resulting in the identification of 15 haplotypes, 10 haplotypes in the cattle and 5 in humans. No haplotype was shared between cattle and human parasites.


CONCLUSION
Human-derived Blastocystis subtypes were different from cattle subtypes in southern Iran. Nevertheless, subtype 5 in cattle can be a risk factor for human infection.
['Sharifi|Yosef|Y|', 'Abbasi|Ferdos|F|', 'Shahabi|Saeed|S|', 'Zaraei|Afshin|A|', 'Mikaeili|Fattaneh|F|', 'Sarkari|Bahador|B|']
D000818:Animals D016844:Blastocystis D016776:Blastocystis Infections D002417:Cattle D002418:Cattle Diseases D016054:DNA, Protozoan D005243:Feces D014644:Genetic Variation D005838:Genotype D006801:Humans D007492:Iran D010802:Phylogeny
Genotype Blastocystis Cattle Humans South of Iran