New Bisabosquals from Stachybotrys sp. PH30583 Elicited on Solid Media.
Stachybotrys sp. PH30583 cultured in liquid medium only led to one structure type of novel isochroman dimers. Using the one strain-many compounds strategy, the reinvestigation of the metabolites from Stachybotrys sp. PH30583 cultured in rice solid medium led to the isolation of four triprenyl phenols, including two new bisabosquals and two known phenylspirodrimanes. Nitrobisabosquals A and B (1 and 2) are the first case of pyrrolidone-bisabosquals reported in literature. Totally different compounds were isolated using rice solid medium, compared with those isolated using liquid medium, so that rice solid medium presents a key factor in the production of triprenyl phenols. Compound 1 exhibited cytotoxicity against tumor cells, A-549, HL-60, MCF-7 SMMC-7721, and SW480, as well as weak anticoagulant activity with activated partial thromboplastin time (APTT) of 32.1 ± 0.17 s (p < 0.05 vs. Con.) at a concentration of 5 mM. Triprenyl phenol metabolites could be used as chemotaxonomic markers for Stachybotrys.
['Ruan|Bao-Hui|BH|', 'Li|Shu-Quan|SQ|', 'Yang|Xue-Qiong|XQ|', 'Yang|Ya-Bin|YB|', 'Wu|Ya-Mei|YM|', 'Shi|Li-Jiao|LJ|', 'Yin|Hai-Yue|HY|', 'Zhou|Hao|H|', 'Ding|Zhong-Tao|ZT|']
D000925:Anticoagulants D000970:Antineoplastic Agents D045744:Cell Line, Tumor D006801:Humans D009682:Magnetic Resonance Spectroscopy D015394:Molecular Structure D010636:Phenols D021241:Spectrometry, Mass, Electrospray Ionization D013191:Stachybotrys
Stachybotrys sp. PH30583 triprenyl phenol cytotoxicity anticoagulant activity chemotaxonomic marker medium induction