Golgi phosphoprotein 3 regulates metastasis of prostate cancer via matrix metalloproteinase 9.
Recent studies suggest that Golgi phosphoprotein 3 (GOLPH3) protein is a candidate metastasis gene in human cancer. The goal of this study was to determine the function of GOLPH3 in prostate cancer metastasis and to identify GOLPH3-regulated pathways and genes involved in prostate cancer metastasis. GOLPH3 expression was detected in prostate cancer. To investigate its function, PC-3 cells were stably transfected with shRNA targeting GOLPH3. Cell abilities of invasion and migration were measured in vitro. Downstream regulatory pathways of GOLPH3 were characterized using quantitative RT-PCR and Western blotting analysis. Immunohistochemical studies in prostate cancer specimens revealed a positive correlation of GOLPH3 expression with prostate cancer. GOLPH3 was expressed in prostate cancer cell lines. GOLPH3 repression resulted in the reduction of mRNA level and protein level of MMP9, accompanied with reduced phosphorylation of mTOR, EGFR and Src. Our findings suggest GOLPH3 regulate MMP9 expression which impact cell migration and invasion. This regulation is probably mediated by EGFR and Src signaling pathways.
['Li|Wenzhi|W|', 'Qi|Kai|K|', 'Wang|Zhanyu|Z|', 'Gu|Meng|M|', 'Chen|Gang|G|', 'Guo|Fengfu|F|', 'Wang|Zhong|Z|']
D045744:Cell Line, Tumor D002465:Cell Movement D066246:ErbB Receptors D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008297:Male D020780:Matrix Metalloproteinase 9 D008565:Membrane Proteins D009362:Neoplasm Metastasis D010766:Phosphorylation D011471:Prostatic Neoplasms D015398:Signal Transduction D058570:TOR Serine-Threonine Kinases D019061:src-Family Kinases
metastasis prostate cancer GOLPH3 MMP9 mTOR EGFR