Feasibility study for combination of field-flow fractionation (FFF)-based separation of size-coded particle probes with amplified surface enhanced Raman scattering (SERS) tagging for simultaneous detection of multiple miRNAs.
We propose a new analytical scheme in which field-flow fractionation (FFF)-based separation of target-specific polystyrene (PS) particle probes of different sizes are incorporated with amplified surface-enhanced Raman scattering (SERS) tagging for the simultaneous and sensitive detection of multiple microRNAs (miRNAs). For multiplexed detection, PS particles of three different diameters (15, 10, 5 μm) were used for the size-coding, and a probe single stranded DNA (ssDNA) complementary to a target miRNA was conjugated on an intended PS particle. After binding of a target miRNA on PS probe, polyadenylation reaction was executed to generate a long tail composed of adenine (A) serving as a binding site to thymine (T) conjugated Au nanoparticles (T-AuNPs) to increase SERS intensity. The three size-coded PS probes bound with T-AuNPs were then separated in a FFF channel. With the observation of extinction-based fractograms, separation of three size-coded PS probes was clearly confirmed, thereby enabling of measuring three miRNAs simultaneously. Raman intensities of FFF fractions collected at the peak maximum of 15, 10 and 5 μm PS probes varied fairy quantitatively with the change of miRNA concentrations, and the reproducibility of measurement was acceptable. The proposed method is potentially useful for simultaneous detection of multiple miRNAs with high sensitivity.
['Shin|Kayeong|K|', 'Choi|Jaeyeong|J|', 'Kim|Yeoju|Y|', 'Lee|Yoonjeong|Y|', 'Kim|Joohoon|J|', 'Lee|Seungho|S|', 'Chung|Hoeil|H|']
D015342:DNA Probes D004277:DNA, Single-Stranded D005240:Feasibility Studies D031521:Fractionation, Field Flow D006046:Gold D035683:MicroRNAs D053758:Nanoparticles D010316:Particle Size D011137:Polystyrenes D015203:Reproducibility of Results D013059:Spectrum Analysis, Raman
Surface-enhanced Raman scattering (SERS) MicroRNAs Multiplexed detection Polyadenylation Gravitational field-flow fractionation (GrFFF) Size-coded probes