Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3.
Tumor suppressor p53-directed apoptosis triggers loss of normal cells, which contributes to the side-effects from anticancer therapies. Thus, small molecules with potential to downregulate the activation of p53 could minimize pathology emerging from anticancer therapies. Acetylation of p53 by the histone acetyltransferase (HAT) domain is the hallmark of coactivator CREB-binding protein (CBP) epigenetic function. During genotoxic stress, CBP HAT-mediated acetylation is essential for the activation of p53 to transcriptionally govern target genes, which control cellular responses. Here, we present a small molecule, NiCur, which blocks CBP HAT activity and downregulates p53 activation upon genotoxic stress. Computational modeling reveals that NiCur docks into the active site of CBP HAT. On CDKN1A promoter, the recruitment of p53 as well as RNA Polymerase II and levels of acetylation on histone H3 were diminished by NiCur. Specifically, NiCur reduces the levels of acetylation at lysine 27 on histone H3, which concomitantly increases the levels of trimethylation at lysine 27. Finally, NiCur attenuates p53-directed apoptosis by inhibiting the Caspase 3 activity and cleavage of Poly (ADP-ribose) polymerase (PARP) in normal gastrointestinal epithelial cells. Collectively, NiCur demonstrates the potential to reprogram the chromatin landscape and modulate biological outcomes of CBP-mediated acetylation under normal and disease conditions.
['Vincek|Adam S|AS|', 'Patel|Jigneshkumar|J|', 'Jaganathan|Anbalagan|A|', 'Green|Antonia|A|', 'Pierre-Louis|Valerie|V|', 'Arora|Vimal|V|', 'Rehmann|Jill|J|', 'Mezei|Mihaly|M|0000-0003-0294-4307', 'Zhou|Ming-Ming|MM|', 'Ohlmeyer|Michael|M|', 'Mujtaba|Shiraz|S|']
D000107:Acetylation D000818:Animals D017209:Apoptosis D050882:CREB-Binding Protein D045744:Cell Line, Tumor D049109:Cell Proliferation D002843:Chromatin D003474:Curcumin D004249:DNA Damage D015536:Down-Regulation D004353:Drug Evaluation, Preclinical D020895:Enterocytes D004791:Enzyme Inhibitors D006657:Histones D006801:Humans D020128:Inhibitory Concentration 50 D008239:Lysine D008745:Methylation D000072417:Protein Domains D051381:Rats D013329:Structure-Activity Relationship D016159:Tumor Suppressor Protein p53
histone acetyltransferase methylation cy-C5-curcuminoids histone acetylation lysine acetylation p53 acetylation transcriptional coactivator CBP