Characteristics of clinical and environmental vanM-carrying vancomycin-resistant enterococci isolates from an infected patient.
vanM, an uncommon glycopeptide resistance gene, was first identified in an Enterococcus faecium isolate (Efm-HS0661) from Shanghai, China, in 2006 and has been predominant in this city since 2011. A vanM-carrying E. faecium was isolated from the bloodstream of a patient in an intensive care unit (ICU) in Hangzhou, China, in 2014. Further surveillance screening of a rectal swab and environmental surfaces of the patient yielded a large number of vanM-positive E. faecium. These isolates (including 1 from the bloodstream, 1 from the rectal swab and 43 representative isolates from environmental samples) were classified into four pulsed-field gel electrophoresis (PFGE) patterns and two sequence types (ST78 and ST564). PCR amplification and sequence analysis indicated that the genetic structure surrounding the vanM gene of these isolates was similar to that of the original vanM-carrying isolate Efm-HS0661. This study highlights the emergence of infections and environmental contamination caused by vanM-carrying E. faecium in an ICU of another Chinese city outside of Shanghai.
['Zhang|Gensheng|G|', 'Lin|Xiuhui|X|', 'Cai|Jiachang|J|', 'Zhang|Rong|R|', 'Xiu|Huiqing|H|', 'Tian|Baoping|B|', 'Cui|Wei|W|']
D000900:Anti-Bacterial Agents D001426:Bacterial Proteins D024881:Drug Resistance, Bacterial D016521:Electrophoresis, Gel, Pulsed-Field D016984:Enterococcus faecium D006801:Humans D000069349:Linezolid D008297:Male D008826:Microbial Sensitivity Tests D008875:Middle Aged D014640:Vancomycin D065507:Vancomycin-Resistant Enterococci
Glycopeptide resistance Enterococcus faecium vanM gene Genetic context