Production of rosmarinic acid with ATP and CoA double regenerating system.
Rosmarinic acid (RA), as a hydroxycinnamic acid ester of caffeic acid (CA) and 3,4-dihydroxyphenyllactic acid (3,4-DHPL), is a phenylpropanoid-derived plant natural product and has diverse biological activities. This work acts as a modular platform for microbial production using a two-cofactor (ATP and CoA) regeneration system to product RA based on a cell-free biosynthetic approach. Optimal activity of the reaction system was pH 8 and 30 °C. Total turnover number for ATP and CoA was 820.60 ± 28.60 and 444.50 ± 9.65, respectively. Based on the first hour data, the RA productivity reached 320.04 mg L-1 h-1 (0.889 mM L-1 h-1).
['Yan|Yi|Y|', 'Jia|Pu|P|', 'Bai|Yajun|Y|', 'Fan|Tai-Ping|TP|', 'Zheng|Xiaohui|X|', 'Cai|Yujie|Y|']
D000255:Adenosine Triphosphate D000894:Anti-Inflammatory Agents, Non-Steroidal D002474:Cell-Free System D002934:Cinnamates D003065:Coenzyme A D053630:Depsides D006863:Hydrogen-Ion Concentration D013696:Temperature
Rosmarinic acid ATP CoA Regeneration system Cell-Free