Anti-HBV activity of the different extracts from Phyllanthus rheedei Wight in cell culture based assay systems.
ETHNOPHARMACOLOGICAL RELEVANCE
Phyllanthusrheedei Wight is a plant used by Muthuvan tribes of Kerala for treating liver related diseases.


MATERIALS AND METHODS
The different extracts of Phyllanthus rheedei were analysed on cell lines were viz, PLC/PRF, Hep3B, FLCII10 and HepG2215 for its anti-HBV property. The analysis was done through ELISA, SQRT-PCR and immuno blotting. The most active extract was then divided in to fractions using HPTLC and the most active fraction was further identified.


RESULTS
From the screening experiments it was shown that the ethanol extract of this plant has the maximum activity in lowering the viral markers like HBsAg, HBV Core and HBV X protein and whole virions with comparatively lesser cytotoxicity. The dose responses of this particular extract were further established.


CONCLUSIONS
This study concluded that the ethanol extract of Phyllanthusrheedei is very much effective in preventing the multiplication of HBV at the cellular level. This study scientifically validated the tribal claim of the use of this plant for severe liver disorders.
['Suresh|V|V|', 'Sojan|Jose|J|', 'Krishna Radhika|N|N|', 'Asha|V V|VV|']
D000998:Antiviral Agents D002460:Cell Line D004279:DNA, Viral D006512:Hepatitis B Core Antigens D006514:Hepatitis B Surface Antigens D006515:Hepatitis B virus D008099:Liver D029803:Phyllanthus D010936:Plant Extracts
Anti-HBV Phyllanthus rheedei Wight ELISA SQRT-PCR Immuno blotting Hepatitis