Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense.
Taurine aminotransferases catalyze the first step in taurine catabolism in many taurine-degrading bacteria and play an important role in bacterial taurine metabolism in the mammalian gut. Here, we report the biochemical and structural characterization of a new taurine:2-oxoglutarate aminotransferase from the human gut bacterium Bifidobacterium kashiwanohense (BkToa). Biochemical assays revealed high specificity of BkToa for 2-oxoglutarate as the amine acceptor. The crystal structure of BkToa in complex with pyridoxal 5'-phosphate (PLP) and glutamate was determined at 2.7 Å resolution. The enzyme forms a homodimer, with each monomer exhibiting a typical type I PLP-enzyme fold and conserved PLP-coordinating residues interacting with the PLP molecule. Two glutamate molecules are bound in sites near the predicted active site and they may occupy a path for substrate entry and product release. Molecular docking reveals a role for active site residues Trp21 and Arg156, conserved in Toa enzymes studied to date, in interacting with the sulfonate group of taurine. Bioinformatics analysis shows that the close homologs of BkToa are also present in other anaerobic gut bacteria.
['Li|Mengya|M|', 'Wei|Yifeng|Y|', 'Yin|Jinyu|J|', 'Lin|Lianyun|L|', 'Zhou|Yan|Y|', 'Hua|Gaoqun|G|', 'Cao|Peng|P|', 'Ang|Ee Lui|EL|', 'Zhao|Huimin|H|', 'Yuchi|Zhiguang|Z|', 'Zhang|Yan|Y|0000-0003-2031-0631']
D000595:Amino Acid Sequence D019943:Amino Acid Substitution D001426:Bacterial Proteins D001644:Bifidobacterium D020134:Catalytic Domain D017124:Conserved Sequence D018360:Crystallography, X-Ray D041981:Gastrointestinal Tract D006801:Humans D007700:Kinetics D008958:Models, Molecular D062105:Molecular Docking Simulation D016297:Mutagenesis, Site-Directed D010802:Phylogeny D011732:Pyridoxal Phosphate D011994:Recombinant Proteins D017386:Sequence Homology, Amino Acid D000637:Transaminases
aminotransferase taurine 2-oxoglutarate gut bacteria isethionate sulfoacetaldehyde