A reversible calix[4]arene armed phenolphthalein based fluorescent probe for the detection of Zn2+ and an application in living cells.
A reversible and easy assembled fluorescent sensor based on calix[4]arene and phenolphthalein (C4P) was developed for selective zinc ion (Zn2+ ) sensing in aqueous samples. The probe C4P demonstrated high selective and sensitive detection towards Zn2+ over other competitive metal ions. Interaction of Zn2+ with a solution of C4P resulted in a considerable increment in emission intensity at 440 nm (λex  = 365 nm) due to the suppression of photoinduced electron transfer (PET) process and the restriction of C=N isomerization. The binding constant (Ka ) of C4P with Zn2+ was calculated to be 4.50 × 1011  M-2 and also the limit of detection of C4P for Zn2+ was as low as 0.108 μM (at 10-7  M level). Moreover, the fluorescence imaging in the human colon cancer cells suggested that C4P had great potential to be used to examine Zn2+ in vivo.
['Erdemir|Serkan|S|https://orcid.org/0000-0003-0790-0549', 'Malkondu|Sait|S|', 'Kocyigit|Ozcan|O|']
D002104:Cadmium D047250:Calixarenes D045744:Cell Line, Tumor D003545:Cysteine D005456:Fluorescent Dyes D006801:Humans D007536:Isomerism D057230:Limit of Detection D009682:Magnetic Resonance Spectroscopy D057054:Molecular Imaging D020113:Phenolphthalein D010636:Phenols D012680:Sensitivity and Specificity D013050:Spectrometry, Fluorescence D013056:Spectrophotometry, Ultraviolet D015032:Zinc
calix[4]arene phenolphthalein fluorescent living cell zinc