Claudins in a primary cultured puffer fish (Tetraodon nigroviridis) gill epithelium model alter in response to acute seawater exposure.
Gill epithelium permeability and qualitative/quantitative aspects of gill claudin (cldn) tight junction (TJ) protein transcriptomics were examined with a primary cultured model gill epithelium developed using euryhaline puffer fish (Tetraodon nigroviridis) gills. The model was prepared using seawater-acclimated fish gills and was cultured on permeable cell culture filter supports. The model is composed of 1-2 confluent layers of gill pavement cells (PVCs), with the outer layer exhibiting prominent apical surface microridges and TJs between adjacent cells. During development of electrophysiological characteristics, the model exhibits a sigmoidal increase in transpithelial resistance (TER) and plateaus around 30 kΩcm(2). At this point paracellular movement of [(3)H]polyethylene glycol (PEG) 4000 was low at ~1.75 cm s(-1)×10(-7). When exposed to apical seawater (SW) epithelia exhibit a marked decrease in TER while PEG flux remained unchanged for at least 6 h. In association with this, transcript encoding cldn TJ proteins cldn3c, -23b, -27a, -27c, -32a and -33b increased during the first 6 h while cldn11a decreased. This suggests that these proteins are involved in maintaining barrier properties between gill PVCs of SW fishes. Gill cldn mRNA abundance also altered 6 and 12 h following abrupt SW exposure of puffer fish, but in a manner that differed qualitatively and quantitatively from the cultured model. This most likely reflects the cellular heterogeneity of whole tissue and/or the contribution of the endocrine system in intact fish. The current study provides insight into the physiological and transcriptomic response of euryhaline fish gill cells to a hyperosmotic environment.
['Bui|Phuong|P|', 'Kelly|Scott P|SP|']
D000818:Animals D002478:Cells, Cultured D057167:Claudins D017097:Electric Impedance D055724:Electrophysiological Phenomena D004847:Epithelial Cells D004848:Epithelium D029941:Fish Proteins D020869:Gene Expression Profiling D005880:Gills D008855:Microscopy, Electron, Scanning D046529:Microscopy, Electron, Transmission D011092:Polyethylene Glycols D061251:Primary Cell Culture D020033:Protein Isoforms D012333:RNA, Messenger D020133:Reverse Transcriptase Polymerase Chain Reaction D012623:Seawater D023721:Tetraodontiformes D014316:Tritium
Claudin Gill Permeability Tight junction Pavement cell