Long non-coding RNA AGAP2-AS1 promotes proliferation and metastasis in papillary thyroid cancer by miR-628-5p/KLF12 axis.
Long non-coding RNA (lncRNA) AGAP2-AS1 acts as an oncogene in several types of cancers. However, the role and mechanism of AGAP2-AS1 in papillary thyroid carcinoma (PTC) remain unclear. Thus, in this study, we aimed to explore the role of AGAP2-AS1 in PTC. Our results showed that AGAP2-AS1 was significantly upregulated in PTC tissues. Knockdown of AGAP2-AS1 inhibited the proliferation, migration and invasion of PTC cells. In vivo experiment showed that AGAP2-AS1 knockdown inhibited the tumorigenesis of PTC. MiR-628-5p was found to act as a target miRNA of AGAP2-AS1 in PTC. The expression level of miR-628-5p in PTC tissues was negatively associated with that of AGAP2-AS1. Inhibition of miR-628-5p attenuated the effects of AGAP2-AS1 knockdown on PTC. Moreover, miR-628-5p directly bound to the 3'UTR of KLF12 and inhibited the expression of KLF12. Knockdown of KLF12 enhanced the inhibitory effects of miR-628-5p on PTC cell proliferation and metastasis. In conclusion, these findings indicated that AGAP2-AS1 exerted an oncogenic role in PTC progression and metastasis. The effects of AGAP2-AS1 might be mediated by the regulation of miR-628-5p/KLF12 axis.
['Xu|Chongwen|C|', 'Shao|Yuan|Y|', 'Liu|Junsong|J|', 'Yao|Xiaobao|X|', 'Quan|Fang|F|', 'Zhao|Qian|Q|', 'Zhao|Ruimin|R|', 'Kou|Bo|B|', 'Li|Honghui|H|', 'Han|Peng|P|', 'Wang|Xuan|X|', 'Bai|Yanxia|Y|', 'Zheng|Zhiwei|Z|', 'Zhang|Shaoqiang|S|']
AGAP2-AS1 Metastasis miR-628-5p KLF12 Papillary thyroid carcinoma (PTC) Long non‐coding RNA (lncRNA)