Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis.
Proteins that migrate through cross-linked polyacrylamide gels (PAGs) under the influence of a constant electric field experience negative factors, such as diffusion and nonspecific trapping in the gel matrix. These negative factors reduce protein concentrations within a defined gel volume with increasing migration distance and, therefore, decrease protein recovery efficiency. Here, we describe the enhancement of protein separation efficiency for up to twofold in conventional one-dimensional PAG electrophoresis (1D PAGE), two-dimensional (2D) PAGE, and native PAGE by implementing pulses of inverted electric field during gel electrophoresis.
['Tsai|Henghang|H|', 'Leung|Hon-Chiu Eastwood|HE|']
D003432:Cross-Linking Reagents D016521:Electrophoresis, Gel, Pulsed-Field D008970:Molecular Weight D011506:Proteins
Field-inversion gel electrophoresis Separation efficiency Forward pulse time Pulsed-field gel electrophoresis Reverse pulse time Two-dimensional polyacrylamide gel electrophoresis