Biocompatibility assessment of graphene oxide-hydroxyapatite coating applied on TiO2 nanotubes by ultrasound-assisted pulse electrodeposition.
In this study, the ultrasound-assisted pulse electrodeposition was introduced to fabricate the graphene oxide (GO)-hydroxyapatite (HA) coating on TiO2 nanotubes. The results of the X-ray diffraction (XRD), Fourier Transform Infrared spectroscope (FTIR), Transmission Electron Microscope (TEM) and micro-Raman spectroscopy showed the successful synthesis of GO. The Scanning Electron Microscope (SEM) images revealed that in the presence of ultrasonic waves and GO sheets a more compact HA-based coating with refined microstructure could be formed on the pretreated titanium. The results of micro-Raman analysis confirmed the successful incorporation of the reinforcement filler of GO into the coating electrodeposited by the ultrasound-assisted method. The FTIR analysis showed that the GO-HA coating was consisted predominantly of the B-type carbonated HA (CHA) phase. The pretreatment of the substrate and incorporation of the GO sheets into the HA coating had a significant effect on improving the bonding strength at the coating-substrate interface. Moreover, the results of the fibroblast cell culture and 3‑(4,5‑dimethylthiazolyl‑2)‑2, 5‑diphenyltetrazolium bromide (MTT) assay after 2 days demonstrated a higher percentage of cell activity for the GO-HA coated sample. Finally, the 7-day exposure to simulated body fluid (SBF) showed a faster rate of apatite precipitation on the GO-HA coating, as compared to the HA coating and pretreated titanium.
['Fathyunes|Leila|L|', 'Khalil-Allafi|Jafar|J|', 'Sheykholeslami|Seyed Omid Reza|SOR|', 'Moosavifar|Maryam|M|']
D000818:Animals D002460:Cell Line D020099:Coated Materials, Biocompatible D017886:Durapatite D055664:Electrochemical Techniques D005347:Fibroblasts D006108:Graphite D008422:Materials Testing D051379:Mice D014025:Titanium D000069453:Ultrasonic Waves
Biocompatibility Graphene oxide Simulated body fluid Hydroxyapatite Ultrasound-assisted electrodeposition