Development of a Swine Benign Biliary Stricture Model Using Endoscopic Biliary Radiofrequency Ablation.
The large animal model with benign biliary stricture (BBS) is essential to undergo experiment on developing new devices and endoscopic treatment. This study conducted to establish a clinically relevant porcine BBS model by means of endobiliary radiofrequency ablation (RFA). Endoscopic retrograde cholangiography (ERC) was performed on 12 swine. The animals were allocated to three groups (60, 80, and 100 W) according to the electrical power level of RFA electrode. Endobiliary RFA was applied to the common bile duct for 60 seconds using an RFA catheter that was endoscopically inserted. ERC was repeated two and four weeks, respectively, after the RFA to identify BBS. After the strictures were identified, histologic evaluations were performed. On the follow-up ERC two weeks after the procedure, a segmental bile duct stricture was observed in all animals. On microscopic examination, severe periductal fibrosis and luminal obliteration with transmural inflammation were demonstrated. Bile duct perforations occurred in two pigs (100 W, n = 1; 80 W, n = 1) but there were no major complications in the 60 W group. The application of endobiliary RFA with 60 W electrical power resulted in a safe and reproducible swine model of BBS.
['Park|Jin Seok|JS|http://orcid.org/0000-0001-9911-8823', 'Jeong|Seok|S|http://orcid.org/0000-0001-6178-8338', 'Kim|Joon Mee|JM|http://orcid.org/0000-0003-1355-4187', 'Park|Sang Soon|SS|http://orcid.org/0000-0003-1609-1990', 'Lee|Don Haeng|DH|http://orcid.org/0000-0003-0397-302X']
D000818:Animals D017115:Catheter Ablation D002760:Cholangiopancreatography, Endoscopic Retrograde D003135:Common Bile Duct D003251:Constriction, Pathologic D005260:Female D005355:Fibrosis D007249:Inflammation D023421:Models, Animal D013552:Swine
Endoscopes Radiofrequency Ablation Animal Experimentation Benign Stricture Safety