Analysis of RNA-DNA Triplex Structures In Vitro and In Vivo.
RNA can bind within the major groove of purine-rich DNA via Hoogsteen base pairing and form a triple helical RNA-DNA structure that anchors the RNA to specific DNA sequences, thereby targeting RNA-associated regulatory proteins to distinct genomic sites. Here we present methods to analyze the potential of a given RNA to form triplexes in vitro and to validate these structures in vivo.
['Postepska-Igielska|Anna|A|', 'Blank-Giwojna|Alena|A|', 'Grummt|Ingrid|I|']
D004247:DNA D024202:Electrophoretic Mobility Shift Assay D006367:HeLa Cells D006801:Humans D009690:Nucleic Acid Conformation D012313:RNA
Biotinylated RNA Electrophoretic mobility shift assay Noncoding RNA Psoralen Pull-down RNA–DNA triplexes Triplex capture