Cell-free therapy with the secretome of adipose tissue-derived stem cells in rats' frozen-thawed ovarian grafts.
The use of secretome may be a new strand of cell therapy, which is equal to or even superior to the injection of live cells, called cell-free therapy. In ovarian transplantation, this approach may be a therapeutic possibility for the ovarian graft in hypoxia. We designed the present study to evaluate whether the cell-free therapy with the secretome of adipose tissue-derived stem cells (ASCs) in rat frozen-thawed ovarian grafts could protect a graft against ischemic injury. A single dose of rat ASCs secretome or vehicle was injected into the bilateral frozen-thawed ovaries of 18 adult female rats immediately after an autologous transplant. Nine animals were used to control the cryopreservation protocol and were evaluated before and after the cryopreservation process. Daily vaginal smears were performed for estrous cycle evaluation until euthanasia on postoperative day 30. Follicle viability by trypan blue, graft morphology by HE, and apoptosis by TUNEL and cleaved-caspase-3 were assessed. No differences were found with respect to estrous cycle resumption and follicle viability (p > 0.05). However, compared with the vehicle-treated grafts, the morphology of the secretome-treated grafts was impaired, showing reduced follicular population and increased apoptosis (p < 0.05). ASC secretome impaired the rat frozen-thawed ovarian graft from ischemic injury. However, more studies are needed to evaluate the factors involved and the possibility of applying the secretome in scaffolds to optimize its use.
['Damous|Luciana Lamarão|LL|0000-0002-2687-7332', 'de Carvalho|Ana Elisa Teófilo Saturi|AETS|', 'Nakamuta|Juliana Sanajotti|JS|', 'Shiroma|Marcos Eiji|ME|', 'Louzada|Andressa Cristina Sposato|ACS|', 'Soares-Jr|José Maria|JM|', 'Krieger|José Eduardo|JE|', 'Baracat|Edmund C|EC|']
D000273:Adipose Tissue D000818:Animals D015925:Cryopreservation D005260:Female D006801:Humans D000860:Hypoxia D007511:Ischemia D045164:Mesenchymal Stem Cell Transplantation D010053:Ovary D051381:Rats D017208:Rats, Wistar D014182:Transplantation, Autologous
Secretome Stem cells Rat Ovarian transplantation Fertility preservation