Nutrient control for stationary phase cellulase production in Trichoderma reesei Rut C-30.
This work describes the use of nutrient limitations with Trichoderma reesei Rut C-30 to obtain a prolonged stationary phase cellulase production. This period of non-growth may allow for dependable cellulase production, extended fermentation periods, and the possibility to use pellet morphology for easy product separation. Phosphorus limitation was successful in halting growth and had a corresponding specific cellulase production of 5±2 FPU/g-h. Combined with the addition of Triton X-100 for fungal pellet formation and low shear conditions, a stationary phase cellulase production period in excess of 300 h was achieved, with a constant enzyme production rate of 7±1 FPU/g-h. While nitrogen limitation was also effective as a growth limiter, it, however, also prevented cellulase production.
['Callow|Nicholas V|NV|', 'Ray|Christopher S|CS|', 'Kelbly|Matthew A|MA|', 'Ju|Lu-Kwang|LK|']
D000645:Ammonium Sulfate D018533:Biomass D002480:Cellulase D003470:Culture Media D003902:Detergents D005285:Fermentation D005656:Fungal Proteins D007218:Industrial Microbiology D007785:Lactose D060847:Metabolic Engineering D009172:Mycology D009584:Nitrogen D017830:Octoxynol D010710:Phosphates D010758:Phosphorus D011189:Potassium Chloride D012212:Rheology D014242:Trichoderma
Cellulase Trichoderma reesei Pellet Phosphorus Nitrogen