3D Hanging Drop Culture to Establish Prostate Cancer Organoids.
Three-dimensional (3D) cell culture enables the growth of cells in a multidimensional and multicellular manner compared to conventional cell culture techniques. Especially in prostate cancer research there is a big need for more tissue-recapitulating models to get a better understanding of the mechanisms driving prostate cancer as well as to screen for more efficient drugs that can be used for treatment. In this chapter we describe a 3D hanging drop system that can be used to culture prostate cancer organoids as tumor epithelial monocultures and as epithelial-stromal cocultures.
['Eder|Theresa|T|', 'Eder|Iris E|IE|']
D018929:Cell Culture Techniques D045744:Cell Line, Tumor D018920:Coculture Techniques D006801:Humans D008297:Male D009940:Organoids D011471:Prostatic Neoplasms D018874:Spheroids, Cellular
Hanging drops Prostate cancer Organoids 3D culture Cell viability assay Drug screening Epithelial–stromal coculture Flow cytometry Immunohistochemistry