Differential proinflammatory responses induced by diesel exhaust particles with contrasting PAH and metal content.
Exposure to diesel engine exhaust particles (DEPs), representing a complex and variable mixture of components, has been linked with cellular production and release of several types of mediators related to pulmonary inflammation. A key challenge is to identify the specific components, which may be responsible for these effects. The aim of this study was to compare the proinflammatory potential of two DEP-samples with contrasting contents of polycyclic aromatic hydrocarbons (PAHs) and metals. The DEP-samples were compared with respect to their ability to induce cytotoxicity, expression and release of proinflammatory mediators (IL-6, IL-8), activation of mitogen-activated protein kinases (MAPKs) and expression of CYP1A1 and heme oxygenase-1 (HO-1) in human bronchial epithelial (BEAS-2B) cells. In addition, dithiothreitol and ascorbic acid assays were performed in order to examine the oxidative potential of the PM samples. The DEP-sample with the highest PAH and lowest metal content was more potent with respect to cytotoxicity and expression and release of proinflammatory mediators, CYP1A1 and HO-1 expression and MAPK activation, than the DEP-sample with lower PAH and higher metal content. The DEP-sample with the highest PAH and lowest metal content also possessed a greater oxidative potential. The present results indicate that the content of organic components may be determinant for the proinflammatory effects of DEP. The findings underscore the importance of considering the chemical composition of particulate matter-emissions, when evaluating the potential health impact and implementation of air pollution regulations.
['Totlandsdal|Annike I|AI|', 'Låg|Marit|M|', 'Lilleaas|Edel|E|', 'Cassee|Flemming|F|', 'Schwarze|Per|P|']
D000393:Air Pollutants D000975:Antioxidants D002460:Cell Line D002470:Cell Survival D019363:Cytochrome P-450 CYP1A1 D051547:Heme Oxygenase-1 D006801:Humans D007249:Inflammation D018836:Inflammation Mediators D008670:Metals D020928:Mitogen-Activated Protein Kinases D052638:Particulate Matter D011084:Polycyclic Aromatic Hydrocarbons D012333:RNA, Messenger D001335:Vehicle Emissions
PAH metals diesel engine exhaust particles inflammation epithelial lung cells