Isolation and Genome Phylogenetic Analysis of Arthropod-Borne Viruses, Including Akabane Virus, from Mosquitoes Collected in Hunan Province, China.
This study investigated the abundance of mosquitoes and circulation of mosquito-borne arboviruses from 16 villages in 8 cities of Hunan Province, China, in July-August of 2010 and in August of 2011. In total, 16,076 mosquitoes consisting of seven species from four genera were collected by ultraviolet-light trap. Culex quinquefasciatus was the most common species, accounting for 50.63% (8140/16,076) of the total. Anopheles sinensis (24.26%, 3900/16,076) made up the second most common species, followed by Culex tritaeniorhynchus (9.76%, 1569/16,076). The proportions of Culex pipiens pallens, Armigeres subalbatus, and Culex modestus were 6.7%, 5.2%, and 3.31%, respectively. Fourteen Aedes albopictus were detected. The mosquitoes were identified by morphologic characteristics and frozen in liquid nitrogen. The mosquitoes were pooled, triturated, and centrifuged. The clarified supernatant was used to inoculate monolayers of C6/36 and baby hamster kidney-21 cells. We obtained six virus isolates that caused cytopathic effects. Phylogenetic analysis revealed that two isolates were Akabane virus (AKAV, from A. sinensis and C. quinquefasciatus), two isolates were Japanese encephalitis virus (from C. pipiens pallens and C. quinquefasciatus), and two isolates were Tibet orbivirus (from C. quinquefasciatus and C. tritaeniorhynchus). This is the first report of AKAV isolated from A. sinensis and C. quinquefasciatus in nature in China. The detection of AKAV in these species confirms circulation of AKAV in Hunan province and suggests potential challenges to the prevention and control of arthropod-borne animal viruses in mainland China.
['Cao|Yuxi|Y|', 'Fu|Shihong|S|', 'Song|Song|S|', 'Cai|Liang|L|', 'Zhang|Hong|H|', 'Gao|Lidong|L|', 'Cao|Lei|L|', 'Li|Minghua|M|', 'Gao|Xiaoyan|X|', 'He|Ying|Y|', 'Wang|Huanyu|H|', 'Liang|Guodong|G|']
D000818:Animals D002460:Cell Line D002681:China D006224:Cricetinae D009033:Culicidae D000072138:Mosquito Vectors D010802:Phylogeny D012367:RNA, Viral D013045:Species Specificity D014780:Viruses
Akabane virus Japanese encephalitis virus Tibet orbivirus arbovirus surveillance mosquito-borne virus