A single-view field filter device for rare tumor cell filtration and enumeration.
In this work, we demonstrate a single-view field filter (SVFF) device for the efficient filtration and enumeration of rare tumor cells in the blood. In our device, the track-etched membrane is integrated within a low-cost polymer-film microfluidic chip, and multiplex microfiltration chambers are designed. Our device permits the performing of multiple sample tests on a single membrane and the dynamical observation of the entire filtration process in a single field of view. To characterize the device performance, our device is first tested using tumor cells, and three different cell behaviors are observed during the filtration process. Finally, we successfully apply our device for the separation of rare tumor cells from the lysed blood samples at various flow rates. The recovery rates of 93.3, 87.6, and 84.1% can be respectively achieved at the throughputs of 50, 100, and 150 μL/min. Our single-view field filter (SVFF) device offers the advantages of label-free filtration, efficient enumeration, easy integration, and low cost, and holds the potential to be used as an efficient tool for the filtration and enumeration of rare cells.
['Quan|Yunlin|Y|', 'Chen|Ke|K|', 'Xiang|Nan|N|0000-0001-9803-4783', 'Ni|Zhonghua|Z|']
D000072283:A549 Cells D001773:Blood Cells D002469:Cell Separation D004867:Equipment Design D005374:Filtration D006801:Humans D046210:Microfluidic Analytical Techniques D009360:Neoplastic Cells, Circulating
CTC enumeration CTC filtration Circulating tumor cell (CTC) Microfluidic device