rBTI extends Caenorhabditis elegans lifespan by mimicking calorie restriction.
Buckwheat trypsin inhibitor (BTI) is a low molecular weight polypeptide extracted from buckwheat. This study examined the effects of BTI on the lifespan of Caenorhabditis elegans (C. elegans) and investigated the mechanism involved. Our results showed that recombinant BTI (rBTI) extended life expectancy by mimicking calorie restriction (CR) in C. elegans. rBTI promoted formation of reactive oxygen species (ROS) via increasing respiration, induced activities of ROS defense enzymes by activating DAF-16, and increased oxidative stress resistance and survival rates. The inhibition of ROS signal by antioxidants reduced rBTI-mediated longevity by up to 65%. Moreover, it was shown that the disruption of daf-2 abolished the extension of the lifespan and the increased ROS. Taken together, these data indicate that rBTI-mediated longevity mimics CR by down-regulating insulin/IGF-1 signaling (IIS) pathway, implying that BTI has the potential to be a novel anti-aging drug.
['Li|Jiao|J|', 'Cui|Xiaodong|X|', 'Wang|Zhuanhua|Z|', 'Li|Yuying|Y|']
D000375:Aging D000818:Animals D017173:Caenorhabditis elegans D031204:Caloric Restriction D004353:Drug Evaluation, Preclinical D004435:Eating D019612:Fagopyrum D007328:Insulin D007334:Insulin-Like Growth Factor I D008136:Longevity D018384:Oxidative Stress D010101:Oxygen Consumption D017382:Reactive Oxygen Species D011994:Recombinant Proteins D015398:Signal Transduction D014361:Trypsin Inhibitors
rBTI Lifespan CR ROS Insulin/IGF-1 signaling