Monitoring of Atg5-Independent Mitophagy.
Mitophagy is a mitochondrial quality control mechanism where damaged and surplus mitochondria are removed by autophagy. There are at least two different mammalian autophagy pathways: the Atg5-dependent conventional pathway and an Atg5-independent alternative pathway; the latter is involved in the erythrocyte mitophagy. In this chapter we describe the various experimental approaches to assess Atg5-indepedndent mitophagy in mammalian cells.
['Arakawa|Satoko|S|', 'Honda|Shinya|S|', 'Torii|Satoru|S|', 'Tsujioka|Masatsune|M|', 'Shimizu|Shigeomi|S|']
D000818:Animals D000071187:Autophagy-Related Protein 5 D001681:Biological Assay D002460:Cell Line D004912:Erythrocytes D005434:Flow Cytometry D055786:Gene Knockout Techniques D017930:Genes, Reporter D006801:Humans D051379:Mice D008928:Mitochondria D063306:Mitophagy D017384:Sequence Deletion D015398:Signal Transduction
Mitophagy Atg5-independent autophagy Erythrocyte maturation Parkin Selective autophagy