miRNA-21 inhibition enhances RANTES and IP-10 release in MCF-7 via PIAS3 and STAT3 signalling and causes increased lymphocyte migration.
MicroRNAs (miRNAs) are a class of small endogenous gene regulators that have been implicated in various developmental and pathological processes. However, the precise identities and functions of miRNAs involved in antitumor immunity are not yet well understood. miRNA-21 is an oncogenic miRNA that can be detected in various tumours. In this study, we report that a miRNA-21 inhibitor enhances the release of chemoattractants RANTES and IP-10 in the MCF-7 breast cancer cell line and results in increased lymphocyte migration. Thus, miRNA-21 is a potential therapeutic target for cancer immunotherapy. We further demonstrated that PIAS3, a protein inhibitor of activated STAT3, is a target of miRNA-21 in MCF-7. Thus, miRNA-21 is a novel miRNA regulating immune cell recruitment, which acts at least in part via its inhibition of PIAS3 expression and oncogenic STAT3 signalling in tumour cells.
['Wang|Zhiyu|Z|', 'Han|Jinxiang|J|', 'Cui|Yazhou|Y|', 'Zhou|Xiaoyan|X|', 'Fan|Kaixi|K|']
D001943:Breast Neoplasms D045744:Cell Line, Tumor D002465:Cell Movement D018946:Chemokine CCL5 D054357:Chemokine CXCL10 D015536:Down-Regulation D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008214:Lymphocytes D035683:MicroRNAs D018832:Molecular Chaperones D050828:Protein Inhibitors of Activated STAT D034622:RNA Interference D050796:STAT3 Transcription Factor D015398:Signal Transduction
PIAS3 STAT3 microRNA Cancer immunotherapy Chemokine Immunoresistance