Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana.
Circular RNAs (circRNAs) are endogenous noncoding RNAs with covalently closed continuous loop structures that are formed by 3'-5' ligation during splicing. These molecules are involved in diverse physiological and developmental processes in eukaryotic cells. Jasmonic acid (JA) is a critical hormonal regulator of plant growth and defense. However, the roles of circRNAs in the JA regulatory network are unclear. In this study, we performed high-throughput sequencing of Arabidopsis thaliana at 24 h, 48 h, and 96 h after methyl JA (MeJA) treatment. A total of 8588 circRNAs, which were distributed on almost all chromosomes, were identified, and the majority of circRNAs had lengths between 200 and 800 bp. We identified 385 differentially expressed circRNAs (DEcircRNAs) by comparing data between MeJA-treated and untreated samples. Gene Ontology (GO) enrichment analysis of the host genes that produced the DEcircRNAs showed that the DEcircRNAs are mainly involved in response to stimulation and metabolism. Additionally, some DEcircRNAs were predicted to act as miRNA decoys. Eight DEcircRNAs were validated by qRT-PCR with divergent primers, and the junction sites of five DEcircRNAs were validated by PCR analysis and Sanger sequencing. Our results provide insight into the potential roles of circRNAs in the MeJA regulation network.
['Zhang|Jingjing|J|', 'Liu|Ruiqi|R|', 'Zhu|Yanfeng|Y|', 'Gong|Jiaxin|J|', 'Yin|Shuwei|S|', 'Sun|Peisen|P|', 'Feng|Hao|H|', 'Wang|Qi|Q|', 'Zhao|Shuaijing|S|', 'Wang|Zhongyuan|Z|', 'Li|Guanglin|G|']
D000085:Acetates D017360:Arabidopsis D002874:Chromosome Mapping D019295:Computational Biology D003517:Cyclopentanes D020869:Gene Expression Profiling D018506:Gene Expression Regulation, Plant D063990:Gene Ontology D053263:Gene Regulatory Networks D035683:MicroRNAs D054883:Oxylipins D010937:Plant Growth Regulators D000079962:RNA, Circular D012333:RNA, Messenger
circRNAs Arabidopsis thaliana jasmonic acid miRNA decoys GO enrichment