Toxic effects of bisphenol A diglycidyl ether and derivatives in human placental cells.
BADGE (bisphenol A diglycidyl ether) is a synthesis product of bisphenol A (BPA), which, like other plasticizers, can cross the human placenta and reach the foetus. However, compared to BPA, there is almost no toxicological information. This work investigates the toxicity, endocrine and lipid disruption potential of BADGE and its hydrolysed and chlorinated derivatives (BADGE·H2O and BADGE·2HCl) in human placental JEG-3 cells. The analysis of culture medium by HPLC-ESI(+)-QqQ evidenced a good bioavailability of BADGE·2HCl and BADGE·H2O, but low stability of BADGE. Regardless, BADGE·2HCl and BADGE showed higher cytotoxicity than BADGE·H2O, which was the only compound that significantly inhibited CYP19 activity (IC50 49 ± 5 μM). JEG-3 cells lipidome analyzed by FIA-ESI(+/-)-Orbitrap was significantly altered by exposure to BADGE·2HCl and BADGE at concentrations at the low μM range. BADGE·2HCl lead to a strong decrease of diacyl- and triacyl-glycerides (DGs,TGs) together with some membrane lipids, while BADGE lead to an accumulation of TGs. The results evidence the ability of BADGE and derivatives to affect placental lipid handling and to modulate placental CYP19 activity (BADGE·H2O) and highlights the need to monitor human exposure to these compounds, at least as intensely as BPA is monitored.
['Marqueño|Anna|A|', 'Pérez-Albaladejo|Elisabet|E|', 'Flores|Cintia|C|', 'Moyano|Encarnación|E|', 'Porte|Cinta|C|']
D001141:Aromatase D047072:Aromatase Inhibitors D001559:Benzhydryl Compounds D002273:Carcinogens D045744:Cell Line, Tumor D002851:Chromatography, High Pressure Liquid D004075:Diglycerides D004852:Epoxy Compounds D005260:Female D006801:Humans D008563:Membrane Lipids D010636:Phenols D010920:Placenta D010968:Plasticizers D011247:Pregnancy D017382:Reactive Oxygen Species D014280:Triglycerides
Bisphenol A diglycidyl ether Cytotoxicity CYP19 JEG-3 cells Lipidome