Performing Axon Orientation Assays with Secreted Semaphorins and Other Guidance Cues.
The guidance of axons within the developing nervous system is orchestrated by a variety of cues that successively and complementary attract or repel axons to achieve a stereotyped wiring of neural circuits. Here we present a version of a method that has been widely used to identify and characterize the effect of guidance cues on the orientation of axons. We describe the coculture, within a three-dimensional environment, of dorsal spinal cord explants together with a cell aggregate secreting a candidate cue and the method to quantify the effect of this cue on axon orientation.
['Delloye-Bourgeois|Céline|C|', 'Moret|Frédéric|F|', 'Castellani|Valérie|V|']
D000818:Animals D001369:Axons D018920:Coculture Techniques D005260:Female D057809:HEK293 Cells D006801:Humans D051379:Mice D011247:Pregnancy D039961:Semaphorins D013116:Spinal Cord
Coculture Spinal cord explants Cell aggregate Axon guidance Plasma clot