Zinc is essential for the transcription function of the PGC-1α/Nrf2 signaling pathway in human primary endometrial stromal cells.
Zinc (Zn) has antioxidant effect in different types of organs and is closely associated with human health. Endometrial receptivity is one of the most important factors in the embryo implantation and development. However, the regulatory mechanism of Zn in endometrium tissue is still unclear. In the study, we found that plasma Zn level is significantly associated with female infertility, which severely affects female reproductive health. Primary endometrial stromal cells were isolated from female endometrium and cultured in the laboratory. Zn chelator TPEN treatment reduced the expression of stem cell markers CD73, CD90, and CD105 and generated reactive oxygen species in endometrial stromal cells. However, pretreatment of Zn (zinc sulfate) is able to prevent TPEN-induced oxidative stress in vitro. By transcriptional profiling and gene ontology analysis, we found that Zn increased the cellular pluripotency signaling and extracellular matrix-receptor interaction, but reduced autophagy, endocytosis, and the nitrogen metabolism pathway. We further discovered the antioxidant function of Zn through the peroxisome proliferator-activated receptor gamma coactivator 1α/nuclear factor erythroid-2-related factor signaling pathway in endometrial stromal cells. Zn supplementation may open up an effective therapeutic approach for patients with oxidative stress-related endometrial diseases.
['Lu|Xiaodan|X|', 'Zhang|Qiang|Q|', 'Xu|Li|L|0000-0002-6055-721X', 'Lin|Xiuying|X|', 'Fu|Jianhua|J|', 'Wang|Xue|X|', 'Liu|Yinong|Y|', 'Lin|Yifan|Y|', 'Li|Bing|B|', 'Wang|Ruobing|R|', 'Liu|Lei|L|', 'Mi|Xuguang|X|', 'Wei|Haifeng|H|', 'Tan|Yan|Y|', 'Fang|Yanqiu|Y|']
D000328:Adult D002470:Cell Survival D002478:Cells, Cultured D004717:Endometrium D005260:Female D006801:Humans D051267:NF-E2-Related Factor 2 D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha D015398:Signal Transduction D017154:Stromal Cells D014158:Transcription, Genetic D055815:Young Adult D015032:Zinc
zinc primary endometrial stromal cells antioxidant thin endometrium