Description of Sphingomonas mesophila sp. nov., isolated from Gastrodia elata Blume.
A Gram-staining-negative, strictly aerobic, non-motile strain, SYSUP0001T, was isolated from tubers of Gastrodia elata Blume. The 16S rRNA gene sequence result indicated that SYSUP0001T represents a member of the genus Sphingomonas, with the highest sequence similarity (97.7 %) to the type strain of Sphingomonasginsengisoli. SYSUP0001T grew at 14-37 °C and pH 6-8, with optimum growth at 28 °C and pH 7. Tolerance to NaCl was up to 3 % (w/v) with optimum growth in the absence of NaCl. The respiratory quinone was Q-10. The major fatty acids were C18 : 1ω7c, Summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), and C16 : 0. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), sphingoglycolipid (SGL), phosphatidylcholine (PC) and four unidentified polar lipids (L). The DNA G+C content was 67.5 %. The average nucleotide identity (ANI) values between SYSUP0001T and closely related members of the genus Sphingomonas were below the cut-off level (95-96 %) for species delineation. On the basis of the phenotypic, phylogenetic and chemotaxonomic characterizations, SYSUP0001T represents a novel species of the genus Sphingomonas, for which the name Sphingomonasmesophila sp. nov. is proposed. The type strain is SYSUP0001T (=KCTC 62179 T=CGMCC 1.16462T).
['Li|Yan-Qiong|YQ|', 'Narsing Rao|Manik Prabhu|MP|', 'Zhang|Hui|H|', 'Guo|Yan-Mei|YM|', 'Dong|Zhou-Yan|ZY|', 'Alkhalifah|Dalal Hussien M|DHM|', 'Hozzein|Wael N|WN|', 'Xiao|Min|M|', 'Li|Wen-Jun|WJ|']
D015373:Bacterial Typing Techniques D001482:Base Composition D002681:China D004269:DNA, Bacterial D005227:Fatty Acids D031668:Gastrodia D010743:Phospholipids D010802:Phylogeny D035281:Plant Tubers D012336:RNA, Ribosomal, 16S D017422:Sequence Analysis, DNA D020578:Sphingomonas D014451:Ubiquinone
Sphingomonas mesophila Gastrodia elataBlume endophyte genome analysis