Absence of nuclease activity in commonly used oxygen-scavenging systems.
OBJECTIVE
Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems.


RESULTS
Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems.
['Gahlon|Hailey L|HL|', 'Poudevigne-Durance|Paul|P|', 'Rueda|David|D|http://orcid.org/0000-0003-4657-6323']
D002374:Catalase D002850:Chromatography, Gel D004247:DNA D003851:Deoxyribonucleases D005949:Glucose Oxidase D062385:Hydroxybenzoates D007202:Indicators and Reagents D010100:Oxygen D010957:Plasmids D011520:Protocatechuate-3,4-Dioxygenase D017382:Reactive Oxygen Species
Oxygen scavenging system Single-molecule imaging Protocatechuic acid Glucose oxidase Catalase Protocatechuate-3,4-dioxygenase