Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of "Solid State Biocatalysis".
Quercetin is a flavonoid largely employed as a phytochemical remedy and a food or dietary supplement. We present here a novel biocatalytic methodology for the preparation of quercetin from plant-derived rutin, with both substrate and product being in mostly an undissolved state during biotransformation. This "solid-state" enzymatic conversion uses a crude enzyme preparation of recombinant rutinosidase from Aspergillus niger yielding quercetin, which precipitates from virtually insoluble rutin. The process is easily scalable and exhibits an extremely high space-time yield. The procedure has been shown to be robust and was successfully tested with rutin concentrations of up to 300 g/L (ca 0.5 M) at various scales. Using this procedure, pure quercetin is easily obtained by mere filtration of the reaction mixture, followed by washing and drying of the filter cake. Neither co-solvents nor toxic chemicals are used, thus the process can be considered environmentally friendly and the product of "bio-quality." Moreover, rare disaccharide rutinose is obtained from the filtrate at a preparatory scale as a valuable side product. These results demonstrate for the first time the efficiency of the "Solid-State-Catalysis" concept, which is applicable virtually for any biotransformation involving substrates and products of low water solubility.
['Kapešová|Jana|J|', 'Petrásková|Lucie|L|', 'Markošová|Kristína|K|', 'Rebroš|Martin|M|', 'Kotik|Michael|M|', 'Bojarová|Pavla|P|', 'Křen|Vladimír|V|0000-0002-1091-4020']
D001234:Aspergillus niger D055162:Biocatalysis D004187:Disaccharides D005656:Fungal Proteins D006026:Glycoside Hydrolases D007218:Industrial Microbiology D010843:Pichia D011794:Quercetin D012431:Rutin
quercetin rutinose rutinosidase rutin Aspergillus niger “solid-state biocatalysis”