Different frequencies of memory B-cells induced by tetanus, botulinum, and heat-labile toxin binding domains.
Along with robust immunogenicity, an ideal vaccine candidate should be able to produce a long lasting protection. In this regard, the frequency of memory B-cells is possibly an important factor in memory B-cell persistency and duration of immunological memory. On this basis, binding domains of tetanus toxin (HcT), botulinum type A1 toxin (HcA), and heat-labile toxin (LTB) were selected as antigen models that induced long-term, midterm and short-term immune memory, respectively. In the present study, the frequency of total memory B-cells after immunization with HcT, HcA and LTB antigens after 90 and 180 days, and also after one booster, in 190 days, was evaluated. The results showed a significant correlation between frequency of total memory B-cells and duration of humoral immunity. Compared to other antigens, the HcT antibody titers and HcT total memory B-cell populations were greater and persistent even after 6 months. At 6 months after the final immunization, all HcT- and HcA-immunized mice survived against tetanus and botulinum toxins, and also LT toxin binding to GM1 ganglioside was blocked in LTB-immunized mice. We conclude the frequency of memory B-cells and their duration are likely a key factor for vaccine memory duration.
['Rezaie|Ehsan|E|', 'Nekoie|Hekmat|H|', 'Miri|Ali|A|', 'Oulad|Gholamreza|G|', 'Ahmadi|Ali|A|', 'Saadati|Mojtaba|M|', 'Bozorgmehr|Mahmood|M|', 'Ebrahimi|Marzieh|M|', 'Salimian|Jafar|J|']
D000818:Animals D000942:Antigens, Bacterial D016175:B-Lymphocyte Subsets D001427:Bacterial Toxins D001905:Botulinum Toxins D004768:Enterotoxins D029968:Escherichia coli Proteins D007156:Immunologic Memory D051379:Mice D013744:Tetanus Toxin D013997:Time Factors
Frequency Memory B-cell Heat-labile toxin Vaccine Tetanus toxin Botulinum toxin