Uniform spheroid formation on a laboratory-made, low cell attachment surface consisting of a chitin sheet.
We utilized the reaction of chitosan with acetic anhydride to form a chitin gel. This gel was then dried, which formed a chitin sheet. The procedure was extremely easy for a biologist unfamiliar with materials engineering. Spheroids derived from HEK293T cells were formed on the chitin sheet, because the spheroids slightly attached and slowly moved on the chitin sheet.
['Inubushi|Yusaku|Y|', 'Tachibana|Akira|A|https://orcid.org/0000-0001-5995-937X']
D000089:Acetic Anhydrides D000818:Animals D002448:Cell Adhesion D002465:Cell Movement D002686:Chitin D048271:Chitosan D018920:Coculture Techniques D003470:Culture Media D005347:Fibroblasts D005780:Gelatin D005782:Gels D057809:HEK293 Cells D006801:Humans D007753:Laboratories D051379:Mice D018874:Spheroids, Cellular D013499:Surface Properties D014783:Viscosity
spheroids low cell attachment surface Chitin gelatin time-lapse observation