Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration.
In this study, bioactive scaffold of nano magnesium phosphate (nMP)/wheat protein (WP) composite (MWC) was fabricated. The results revealed that the MWC scaffolds had interconnected not only macropores (sized 400-600 μm) but also micropores (sized 10-20 μm) on the walls of macropores. The MWC scaffolds containing 40 w% nMP had an appropriate degradability in phosphate-buffered saline and produced a weak alkaline microenvironment. In cell culture experiments, the results revealed that the MWC scaffolds significantly promoted the MC3T3-E1 cell proliferation, differentiation, and growth into the scaffolds. The results of synchrotron radiation microcomputed tomography and analysis of the histological sections of the in vivo implantation revealed that the MWC scaffolds evidently improved the new bone formation and bone defects repair as compared with WP scaffolds. Moreover, it was found that newly formed bone tissue continued to increase with the gradual reduction of materials residual in the MWC scaffolds. Furthermore, the immunohistochemical analysis further offered the evidence of the stimulatory effects of MWC scaffolds on osteogenic-related cell differentiation and new bone regeneration. The results indicated that MWC scaffolds with good biocompability and degradability could promote osteogenesis in vivo, which would have potential for bone tissue repair.
['Xia|Yan|Y|', 'Zhou|Panyu|P|', 'Wang|Fei|F|', 'Qiu|Chao|C|', 'Wang|Panfeng|P|', 'Zhang|Yuntong|Y|', 'Zhao|Liming|L|', 'Xu|Shuogui|S|']
D000818:Animals D001861:Bone Regeneration D002454:Cell Differentiation D049109:Cell Proliferation D002478:Cells, Cultured D005269:Femur D017616:Magnesium Compounds D008297:Male D008422:Materials Testing D051379:Mice D049329:Nanostructures D010012:Osteogenesis D010710:Phosphates D010940:Plant Proteins D011817:Rabbits D054457:Tissue Scaffolds D014908:Triticum D055114:X-Ray Microtomography
degradation nano magnesium phosphate wheat protein bone regeneration composite scaffolds