An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing.
The sustained emergence of new declared bacterial species makes typing a continuous challenge for microbiologists. Molecular biology techniques have a very significant role in the context of bacterial typing, but they are often very laborious, time consuming, and eventually fail when dealing with very closely related species. Spectroscopic-based techniques appear in some situations as a viable alternative to molecular methods with advantages in terms of analysis time and cost. Infrared and mass spectrometry are among the most exploited techniques in this context: particularly, infrared spectroscopy emerged as a very promising method with multiple reported successful applications. This article presents a systematic review on infrared spectroscopy applications for bacterial typing, highlighting fundamental aspects of infrared spectroscopy, a detailed literature review (covering different taxonomic levels and bacterial species), advantages, and limitations of the technique over molecular biology methods and a comparison with other competing spectroscopic techniques such as MALDI-TOF MS, Raman, and intrinsic fluorescence. Infrared spectroscopy possesses a high potential for bacterial typing at distinct taxonomic levels and worthy of further developments and systematization. The development of databases appears fundamental toward the establishment of infrared spectroscopy as a viable method for bacterial typing.
['Quintelas|Cristina|C|', 'Ferreira|Eugénio C|EC|', 'Lopes|João A|JA|', 'Sousa|Clara|C|']
D001419:Bacteria D015373:Bacterial Typing Techniques D006801:Humans D010802:Phylogeny D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization D013055:Spectrophotometry, Infrared
infrared spectroscopy bacterial typing bacteria chemometrics microbiology