Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy.
BACKGROUND/AIM
20-Hydroxyecdystone (20E) is an ecdysteroid hormone which controls molting and reproduction in arthropods. 20E also produces a variety of effects in vertebrates, including enhancing protein synthesis and skeletal muscle regeneration. The effect of 20E on disuse muscle atrophy has not been reported to date. This study examined the proteolytic regulation of 20E in tenotomized rat slow soleus and fast plantaris muscles.


MATERIALS AND METHODS
Male Wistar rats were randomly divided into three groups: sedentary control (CON), tenotomy without 20E treatment (TEN), and tenotomy with treatment of 5 mg/kg BW of 20E (TEN+20E). The TEN+20E group was administered 20E via subcutaneous injection to the right thigh for 7 days after tenotomy.


RESULTS
20E treatment tended to attenuate disuse muscle atrophy and reduced ubiquitination only in soleus muscle.


CONCLUSION
20E treatment alleviates skeletal muscle atrophy partially mediated by ubiquitinate pathway, dependent on the muscle phenotype.
['Hirunsai|Muthita|M|', 'Yimlamai|Tossaporn|T|', 'Suksamrarn|Apichart|A|']
D000818:Animals D015153:Blotting, Western D004441:Ecdysterone D007279:Injections, Subcutaneous D008297:Male D018482:Muscle, Skeletal D009133:Muscular Atrophy D059748:Proteolysis D011897:Random Allocation D017208:Rats, Wistar D015398:Signal Transduction D058971:Tenotomy D057149:Ubiquitinated Proteins D054875:Ubiquitination
Ecdysteroid tenotomy ubiquitin MURF1 muscle-type specific