Platelet rich plasma extract promotes angiogenesis through the angiopoietin1-Tie2 pathway.
Development and regeneration of tissues and organs require precise coordination among endothelial, epithelial and mesenchymal morphogenesis. Angiogenesis plays key roles in normal development, wound healing, recovery from ischemic disease, and organ regeneration. It has been recognized that the combination of various angiogenic factors in an appropriate physiological ratio is critical for long-term functional blood vessel formation. Here we show that mouse soluble platelet-rich-plasma (PRP) extract, which includes abundant angiopoetin-1 (Ang1) and other angiogenic factors, stimulates endothelial cell growth, migration and differentiation in cultured human dermal microvascular endothelial cells in vitro and neonatal mouse retinal angiogenesis in vivo. Mouse platelet rich fibrin (PRF) matrix, the three-dimensional fibrin matrix that releases angiogenic factors with similar concentrations and proportions to the PRP extract, also recapitulates robust angiogenesis inside the matrix when implanted subcutaneously on the living mouse. Inhibition of Ang1-Tie2 signaling suppresses PRP extract-induced angiogenesis in vitro and angiogenic ability of the PRF matrix in vivo. Since human PRP extract and PRF matrix can be prepared from autologous peripheral blood, our findings may lead to the development of novel therapeutic interventions for various angiogenesis-related diseases as well as to the improvement of strategies for tissue engineering and organ regeneration.
['Mammoto|Tadanori|T|', 'Jiang|Amanda|A|', 'Jiang|Elisabeth|E|', 'Mammoto|Akiko|A|']
D042683:Angiopoietin-1 D000818:Animals D049109:Cell Proliferation D042783:Endothelial Cells D018507:Gene Expression Regulation, Developmental D006801:Humans D051379:Mice D008810:Mice, Inbred C57BL D008833:Microcirculation D008856:Microscopy, Fluorescence D018919:Neovascularization, Physiologic D053657:Platelet-Rich Plasma D034622:RNA Interference D042787:Receptor, TIE-2 D012038:Regeneration D012160:Retina D012171:Retinal Vessels
platelet rich plasma PRP endothelial cell human dermal microvascular endothelial platelet rich fibrin PRF 3D ARDS Ang BrdU D-HMVE EBM2 EC ECM ELISA FDA Food and Drug Administration H&E IF IP PD-EGF PDGF PDMS TGF-β VEGF VEGFR2 acute respiratory distress syndrome angiopoietin b-FGF basic fibroblast growth factor bromodeoxyuridine conA concanavalin-A endothelial basal medium-2 enzyme-linked immunosorbent assay extracellular matrix hematoxylin and eosin immunofluorescence intraperitoneal platelet-derived epidermal growth factor platelet-derived growth factor polydimethylsiloxane qRT-PCR quantitative reverse transcription polymerase chain reaction three dimensional transforming growth factor beta vascular endothelial growth factor vascular endothelial growth factor receptor 2