Ginsenoside Rg1 prevents cerebral and cerebellar injury induced by obstructive jaundice in rats via inducing expression of TIPE-2.
The aim of the study was to analyze the effect of Ginsenoside Rg1 (Rg1) on cerebral and cerebellar injury in experimental obstructive jaundice (OJ). OJ was done by ligature and section of extrahepatic biliary duct. Rg1 was injected intraperitoneally (10 mg kg(-1)d(-1) or 20 mg kg(-1) d(-1)). Comparison of serum total bile salts (TBA), total bilirubin (TBil), direct bilirubin (DBil), TNF-α, IL-6 and IL-1β among groups. Malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined, also apoptosis and mRNA and protein levels of TIPE2 (TNF-α-inducible protein 8-like 2) were tested in cerebrum and cerebellum. Our results showed that Rg1 reduced MDA and apoptosis in cerebrum and cerebellum induced by OJ, also GSH and antioxidant enzyme activity were raised obviously in rats treated with Rg1. Moreover, decreased mRNA and protein levels of TIPE2 in OJ rats and Rg1 could improve the decreased mRNA and protein levels of TIPE2 in OJ rats. In conclusion, Rg1 decreased oxidative stress and apoptosis, also recovered the antioxidant status and mRNA and protein levels of TIPE2 in the cerebrum and cerebellum of OJ rats.
['Zhou|Tingting|T|', 'Zu|Guo|G|', 'Zhou|Lu|L|', 'Che|Ningwei|N|', 'Guo|Jing|J|', 'Liang|Zhanhua|Z|']
D000818:Animals D017209:Apoptosis D002531:Cerebellum D054022:Cerebrum D036145:Ginsenosides D047908:Intracellular Signaling Peptides and Proteins D041781:Jaundice, Obstructive D008297:Male D018384:Oxidative Stress D012333:RNA, Messenger D017207:Rats, Sprague-Dawley D015854:Up-Regulation
Ginsenoside Rg1 Obstructive jaundice TIPE-2 Apoptosis Cerebrum Cerebellum Oxidative stress