Lipophilic organic pollutants induce changes in phospholipid and membrane protein composition leading to Vero cell morphological change.
Membrane damage related to morphological change in Vero cells is a sensitive index of the composite biotoxicity of trace lipophilic chemicals. However, judging whether the morphological change in Vero cells happens and its ratio are difficult because it is not a quantitative characteristic. To find biomarkers of cell morphological change for quantitatively representing the ratio of morphological changed cell, the mechanism of cell membrane damage driven by typical lipophilic chemicals, such as trichlorophenol (TCP) and perfluorooctanesulphonate (PFOS), was explored. The ratio of morphologically changed cells generally increased with increased TCP or PFOS concentrations, and the level of four major components of phospholipids varied with concentrations of TCP or PFOS, but only the ratio of phosphatidylcholine (PC)/phosphatidylethanolamine (PE) decreased regularly as TCP or PFOS concentrations increased. Analysis of membrane proteins showed that the level of vimentin in normal cell membranes is high, while it decreases or vanishes after TCP exposure. These variations in phospholipid and membrane protein components may result in membrane leakage and variation in rigid structure, which leads to changes in cell morphology. Therefore, the ratio of PC/PE and amount of vimentin may be potential biomarkers for representing the ratio of morphological changed Vero cell introduced by trace lipophilic compounds, thus their composite bio-toxicity.
['Liao|Ting T|TT|', 'Wang|Lei|L|', 'Jia|Ru W|RW|', 'Fu|Xiao H|XH|', 'Chua|Hong|H|']
D017738:Alkanesulfonic Acids D000818:Animals D015415:Biomarkers D002522:Chlorocebus aethiops D002733:Chlorophenols D002851:Chromatography, High Pressure Liquid D002853:Chromatography, Liquid D004591:Electrophoresis, Polyacrylamide Gel D004785:Environmental Pollutants D005466:Fluorocarbons D013058:Mass Spectrometry D008565:Membrane Proteins D010713:Phosphatidylcholines D010714:Phosphatidylethanolamines D014709:Vero Cells D014746:Vimentin
lipophilic organic pollutants phospholipid membrane protein Vero cell morphological change