Interleukin-13 suppresses interleukin-10 via inhibiting A20 in peripheral B cells of patients with food allergy.
The regulatory B cells (Breg) are important in the body immunity. The differentiation process of Breg is not fully understood yet. Ubiquitin A20 has immune regulatory functions. This study aims to investigate the role of A20 in the regulation of interleukin (IL)-10 in B cells. In this study, B cells were isolated from the peripheral blood samples of healthy subjects and patients with food allergy (FA). The B cells were analyzed by flow cytometry, real time RT-PCR, Western blotting and chromatin immunoprecipitation. We observed that the frequency of Breg and the levels of A20 in B cells were markedly lower in FA patients than in healthy controls. In vitro deletion of A20 compromised the expression of IL-10. B cells in FA patients showed higher levels of histone deacetylase (HDAC)-11 than in healthy subjects. Exposure to IL-13 in the culture induced high levels of HDAC11 in B cells. IL-13 also repressed the expression of A20 in B cells, in which HDAC11 played a critical role via inducing the chromatin remoldeling at the IL-10 promoter locus. Mice with A20-deficient B cells are prone to FA. In summary, ubiquitin A20 can increase the IL-10 expression in B cells, which can be affected by the IL-13-induced HDAC11. To inhibit HDAC11 may have therapeutic potential for FA.
['Li|Ming-Yang|MY|', 'Zhu|Min|M|', 'Linghu|En-Qiang|EQ|', 'Feng|Fan|F|', 'Zhu|Bing|B|', 'Wu|Cheng|C|', 'Guo|Ming-Zhou|MZ|']
D000293:Adolescent D000328:Adult D000818:Animals D060151:B-Lymphocytes, Regulatory D002478:Cells, Cultured D015536:Down-Regulation D005260:Female D005512:Food Hypersensitivity D006801:Humans D016753:Interleukin-10 D018793:Interleukin-13 D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D018345:Mice, Knockout D016269:Milk Hypersensitivity D000072598:Tumor Necrosis Factor alpha-Induced Protein 3 D055815:Young Adult
interleukin-13 immunity histone deacetylase immune regulation