Is resveratrol a potential substitute for leuprolide acetate in experimental endometriosis?
OBJECTIVE
Resveratrol, a phytoalexin polyphenol, has anti-angiogenic, antioxidant, anti-inflammatory properties. We aimed to compare the anti-inflammatory and anti-angiogenic effects of resveratrol and leuprolide acetate (LA) in an experimental endometriosis model.


STUDY DESIGN
A prospective experimental study was conducted in a University Surgical Research Center. Thirty-three non-pregnant female Sprague-Dawley rats, in which experimental model of endometriosis were surgically induced were randomly divided into four groups. Group 1 was administered 30 mg/kg resveratrol i.m. for 14 days, group 2 was given 1mg/kg s.c. single dose LA, group 3 was administered both resveratrol and LA, and group 4 had no medication. After two weeks medication rats were sacrificed and size, histopathology and immunreactivity to matrix metalloproteinase (mmp)2, mmp9, vascular endothelial growth factor (VEGF) of the endometriotic implants were evaluated. Plasma and peritoneal fluid levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α (TNF-α) were analyzed.


RESULTS
The endometriotic implant volumes, histopathological grade and immunreactivity to mmp2, mmp9 and VEGF were significantly reduced (p<0.001), and plasma and peritoneal fluid levels of IL-6, IL-8 and TNF-α were significantly decreased in group 1 and group 2 in comparison to group 3 and group 4 (p < 0.001).


CONCLUSION
Resveratrol alone is a potential agent for the treatment of endometriosis and may be an alternative to LA. In contrast, the combination of LA and resveratrol decreased the anti-inflammatory and anti-angiogenic effects of each agent. Since resveratrol is widely used as an alternative therapy for a variety of conditions, it can undermine the effectiveness of LA. Therefore, caution should be exercised when used in combination with other agents.
['Bayoglu Tekin|Yesim|Y|', 'Guven|Suleyman|S|', 'Kirbas|Aynur|A|', 'Kalkan|Yıldıray|Y|', 'Tumkaya|Levent|L|', 'Guvendag Guven|Emine Seda|ES|']
D020533:Angiogenesis Inhibitors D000818:Animals D000894:Anti-Inflammatory Agents, Non-Steroidal D004195:Disease Models, Animal D004715:Endometriosis D004717:Endometrium D005260:Female D016729:Leuprolide D020778:Matrix Metalloproteinase 2 D020780:Matrix Metalloproteinase 9 D051381:Rats D017207:Rats, Sprague-Dawley D000077185:Resveratrol D013267:Stilbenes D016896:Treatment Outcome D042461:Vascular Endothelial Growth Factor A
Resveratrol Leuprolide acetate Endometriosis Treatment Rat model