A high level of lncFGD5-AS1 inhibits epithelial-to-Mesenchymal transition by regulating the miR-196a-5p/SMAD6/BMP axis in gastric Cancer.
BACKGROUND
Long non-coding RNA (lncRNA) was a vital factor in the progression and initiation of human cancers. This study found a new lncRNA, FGD5-AS1, which can inhibit EMT process, proliferation, and metastasis in vitro and in vivo.


METHODS
qRT-PCR was employed to test the expression of lncFGD5-AS1 in 30 gastric cancer patients' cancer tissue and para-cancer tissue. Overexpressed lncFGD5-AS1 cells shown sharply decrease of proliferation, migration, and epithelial-mesenchymal transition (EMT). miR-196a-5p/SMAD6 was confirmed as downstream molecular mechanism of lncFGD5-AS1 by expression correlation analysis and mechanism experiments. In vivo study illustrated overexpression of lncFGD5-AS1 suppression tumor growth.


RESULTS
LncFGD5-AS1 served as a ceRNA of miR-196a-5p to release its inhibition on SMAD6, a conventional inhibitor on the BMP pathway. Comparing with normal gastric cancer cells, FGD5-AS1 overexpressed group had fewer migration cells, lower cell viability, and lower EMT transformation rate. Meanwhile, xenografts nude mice injecting with overexpressed-FGD5-AS1 cells also shown smaller tumor weight and volume.


CONCLUSION
In conclusion, this research supported the first evidence that FGD5-AS1 suppressed proliferation and metastasis in gastric cancer by regulating miR-196a-5p/SMAD6/BMP axis and suggested a potential therapeutic candidate for gastric cancer.
['Liu|Lin|L|', 'Zhang|Cheng|C|', 'Wang|Jizhao|J|', 'Liu|Xu|X|', 'Qu|Hangying|H|', 'Zhang|Guangjian|G|', 'Liang|Ting|T|', 'Wang|Jiansheng|J|', 'Zhang|Jia|J|']
D000818:Animals D052004:Bone Morphogenetic Protein Receptors D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D002470:Cell Survival D058750:Epithelial-Mesenchymal Transition D005260:Female D005753:Gastric Mucosa D020662:Guanine Nucleotide Exchange Factors D006801:Humans D017404:In Situ Hybridization, Fluorescence D008297:Male D051379:Mice D008819:Mice, Nude D035683:MicroRNAs D008875:Middle Aged D009362:Neoplasm Metastasis D011379:Prognosis D062085:RNA, Long Noncoding D020133:Reverse Transcriptase Polymerase Chain Reaction D051905:Smad6 Protein D013274:Stomach Neoplasms D014157:Transcription Factors D047368:Tumor Burden D014410:Tumor Stem Cell Assay
Epithelial-mesenchymal transition4 Prognose of gastric Cancer ceRNA3 lncFGD5-AS11 miR-196a-5p2