Disposition and acute toxicity of imidacloprid in female rats after single exposure.
Single dose of imidacloprid (IMI-20mg/kg bodyweight) was orally administered in female rats. Its disposition along with two metabolites 6-chloro nicotinic acid (6-CNA) and 6-hydroxy nicotinic acid (6-HNA) was monitored in organs (brain, liver, kidney, and ovary) and bodily fluids (blood, urine) at 6, 12, 24 and 48h and faeces at 24 and 48h. Maximum concentration (Cmax) of IMI and metabolites in each organ and bodily fluid occurred after 12h. Area under curve (AUC) of IMI ranged from 35 to 358μg/ml/h; 6-CNA: 27.12-1006.42μg/ml/h and 6-HNA: 14.98-302.74μg/ml/h in different organs and bodily fluids. Clearance rate of IMI was maximum in ovary followed by kidney, liver, brain, faeces, blood and urine. Percent inhibition of acetyl-cholinesterase (AChE) was comparable in brain and Red Blood Cells (RBC) at 6-48h which suggests the RBC-AChE as valid biomarker for assessing IMI exposure. It is evident that IMI was absorbed, metabolized, and excreted showing increased level of serum enzymes like Glutamic oxaloacetic transaminase (GOT), Glutamic pyruvic transaminase (GPT) and biochemical constituents like billirubin and Blood Urea Nitrogen (BUN) at 48h. These data suggest that IMI is widely distributed, metabolized and induced toxicology effects at 20mg/kg bodyweight to female rats.
['Kapoor|Upasana|U|', 'Srivastava|M K|MK|', 'Trivedi|Purushottam|P|', 'Garg|Veena|V|', 'Srivastava|L P|LP|']
D000110:Acetylcholinesterase D000284:Administration, Oral D000410:Alanine Transaminase D000818:Animals D019540:Area Under Curve D001219:Aspartate Aminotransferases D001663:Bilirubin D001806:Blood Urea Nitrogen D001835:Body Weight D001921:Brain D005260:Female D007093:Imidazoles D007668:Kidney D008099:Liver D000073943:Neonicotinoids D009539:Nicotinic Acids D009574:Nitro Compounds D010053:Ovary D051381:Rats D011913:Rats, Inbred BB D023382:Toxicity Tests, Acute
Disposition Toxicity Imidacloprid Female rats 6-CNA 6-HNA