αvβ6-Fyn Kinase Promotes Epithelial Phenotype in SYF Cells.
The 5-year survival rate for patients with oral cancer remains at 50%, in large part due the high rate of post-treatment recurrence. In this study, we transfected epithelial-specific integrin αvβ6 and Fyn-kinase, a member of the Src-family kinases, into embryonic murine fibroblasts. In oral cancer, expression of αvβ6 is neo-expressed. Using a variety of in vitro assays, including cell migration and multicellular spheroid formation, we determined that these embryonic fibroblasts expressing αvβ6 and Fyn-kinase were able to acquire an epithelial phenotype. This is in direct contrast to human oral SCC, where expression of αvβ6 with Fyn-kinase promotes epithelial to mesenchymal transition. This demonstrates that signaling pathways can be species-specific.
['Lee|Casey|C|', 'Ramos|Daniel M|DM|']
D000818:Animals D000951:Antigens, Neoplasm D002460:Cell Line D002465:Cell Movement D004847:Epithelial Cells D058750:Epithelial-Mesenchymal Transition D005347:Fibroblasts D016023:Integrins D051379:Mice D020928:Mitogen-Activated Protein Kinases D010641:Phenotype D051076:Proto-Oncogene Proteins c-fyn
SYF cells migration multicellular spheroid formation Fyn kinase MAPK