Icariin protects against titanium particle-induced osteolysis and inflammatory response in a mouse calvarial model.
Periprosthetic osteolysis and subsequent aseptic loosening are common in implant failure, a complication with revision surgery being the only established treatment. Wear particle-induced inflammation and extensive osteoclastogenesis play critical roles in periprosthetic osteolysis. A recent approach in limiting osteolysis is therefore focused on inhibiting osteoclastic bone resorption. This study aimed to investigate the potential impact of icariin, the major ingredient of Epimedium, on titanium particle-induced osteolysis in a mouse calvarial model. Eighty-four male C57BL/J6 mice were divided randomly into four groups. Mice in the sham group underwent sham surgery only, whereas animals in the vehicle, low- and high-concentration icariin groups received titanium particles. Mice in the low- and high-concentration icariin groups were gavage-fed with icariin at 0.1 or 0.3 mg/g/day, respectively, until sacrifice. Mice in the sham and vehicle groups received phosphate-buffered saline daily. After 2 weeks, mouse calvariae were collected for micro-computed tomography, histomorphometry and molecular analysis. Icariin significantly reduced particle-induced bone resorption compared with the vehicle group. Icariin also prevented an increase in receptor activator of nuclear factor kappa B ligand/osteoprotegerin ratio and subsequently suppressed osteoclast formation in titanium particle-charged calvariae. In addition, immunohistochemical analysis and enzyme-linked immunosorbent assay showed icariin significantly reduced expression and secretion of tumor necrosis factor-α, interleukin-1β and interleukin-6 in the calvariae of titanium-stimulated mice. Collectively, these results suggest that icariin represents a potential treatment for titanium particle-induced osteolysis and could be developed as a new therapeutic candidate for the prevention and treatment of aseptic loosening.
['Shao|Hongguo|H|', 'Shen|Ji|J|', 'Wang|Mingjun|M|', 'Cui|Jingfu|J|', 'Wang|Yijun|Y|', 'Zhu|Shijun|S|', 'Zhang|Wen|W|', 'Yang|Huilin|H|', 'Xu|Yaozeng|Y|', 'Geng|Dechun|D|']
D000818:Animals D004195:Disease Models, Animal D004365:Drugs, Chinese Herbal D034181:Epimedium D005419:Flavonoids D007249:Inflammation D053583:Interleukin-1beta D015850:Interleukin-6 D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D010014:Osteolysis D012886:Skull D014025:Titanium D014409:Tumor Necrosis Factor-alpha
Icariin Aseptic loosening Inflammation Osteoclastogenesis Wear particles