Antioxidant and cytoprotective properties of partridgeberry polyphenols.
Partridgeberry (Vaccinium vitis-idaea) is a polyphenol-rich berry of the Ericaceous family, grown in Newfoundland and Labrador province of Canada. The aims of this study were to identify extraction solvents for the maximum recovery of polyphenols, to establish fractionation technique for isolation of major sub-classes of polyphenols, and to evaluate antioxidant and cytoprotective properties of the partridgeberry polyphenol preparations. The acidified 70% acetone was identified as the ideal solvent for the maximum recovery of polyphenols from partridgeberry. Further, aqueous two-phase extraction, column chromatography and UPLC-MS/MS were employed to produce three partridgeberry polyphenol fractions, rich in either, anthocyanins, flavan-3-ols or flavonols. All the three PPF were potent antioxidants and displayed cytoprotective activity through the activation of nuclear factor erythroid 2-related factor 2 pathway, scavenging of reactive oxygen species, and inhibition of cellular death. The current study suggests that partridgeberry has numerous potential health implications in both prevention and amelioration of various diseases involving oxidative stress.
['Bhullar|Khushwant S|KS|', 'Rupasinghe|H P Vasantha|HP|']
D000872:Anthocyanins D000975:Antioxidants D002460:Cell Line D002470:Cell Survival D002851:Chromatography, High Pressure Liquid D005419:Flavonoids D006801:Humans D059625:Liquid-Liquid Extraction D018384:Oxidative Stress D010936:Plant Extracts D059808:Polyphenols D020011:Protective Agents D017382:Reactive Oxygen Species D053719:Tandem Mass Spectrometry D029801:Vaccinium vitis-idaea
Antioxidant Cytoprotection Partridgeberry Polyphenol Vaccinium vitis-idaea Fractionation (−)-Epicatechin (PubChem CID: 72276) Caffeic acid (PubChem CID: 689043) Catechin (PubChem CID: 9064) Chlorogenic acid (PubChem CID: 1794427) Epigallocatechin (PubChem CID: 72277) Ferulic acid (PubChem CID: 445858) Flavonoids Isoquercitrin (PubChem CID: 25203368) Lingonberry Nrf2 Phloretin (PubChem CID: 4788) Phlorhizin (PubChem CID: 6072) Quercetin (PubChem CID: 5280343)