Distribution of Burkholderia cepacia complex species isolated from industrial processes and contaminated products in Argentina.
Burkholderia cepacia complex (Bcc) members have clinical relevance as opportunistic pathogens in patients with cystic fibrosis and are responsible of numerous nosocomial infections. These closely related bacteria are also reported as frequent contaminants of industrial products. In this retrospective study, we use PCR and recA gene sequence analysis to identify at species level Bcc isolates recovered from massive consumption products and industrial processes in Argentina during the last 25 years. The sequences obtained were also compared with recA sequences from clinical Bcc isolates deposited in GenBank database. We detected Bcc in purified water and preserved products from pharmaceutics, cosmetics, household cleaning articles, and beverages industries. B. contaminans (which is prevalent among people with cystic fibrosis in Argentina) was the most frequent Bcc species identified (42% of the Bcc isolates studied). B. cepacia (10%), B. cenocepacia (5%), B. vietnamiensis (16%), B. arboris (3%), and the recently defined B. aenigmatica (24%) were also detected. Rec A sequences from all B. cepacia and most B. contaminans industrial isolates obtained in this study displayed 100% identity with recA sequences from isolates infecting Argentinean patients. This information brings evidence for considering industrial massive consumption products as a potential source of Bcc infections. In addition, identification at species level in industrial microbiological laboratories is necessary for a better epidemiological surveillance. Particularly in Argentina, more studies are required in order to reveal the role of these products in the acquisition of B. contaminans infections.
['De Volder|A López|AL|https://orcid.org/0000-0001-6072-7234', 'Teves|S|S|https://orcid.org/0000-0002-3938-318X', 'Isasmendi|A|A|', 'Pinheiro|J L|JL|', 'Ibarra|L|L|', 'Breglia|N|N|', 'Herrera|T|T|', 'Vazquez|M|M|', 'Hernandez|C|C|', 'Degrossi|José|J|https://orcid.org/0000-0003-2086-8205']
D001118:Argentina D001426:Bacterial Proteins D001628:Beverages D042602:Burkholderia cepacia complex D003358:Cosmetics D003902:Detergents D005506:Food Contamination D057141:Food, Preserved D010802:Phylogeny D016133:Polymerase Chain Reaction D011938:Rec A Recombinases D012189:Retrospective Studies
Burkholderia cepacia complex Contamination Industrial products recA PCR