Current Methods for the Characterization of O-Glycans.
Glycosylation is crucial in cellular metabolism and survival. Of interest is the role of N-linked and O-linked glycans in disease states. Robust analytical methods must be defined to identify suitable glycan biomarkers and glyco-therapeutics. Fortunately, in N-glycan analysis, a universal enzyme exists to deglycosylate a variety of common-core structures from proteins for analysis using mass spectrometric and fluorescence techniques. Unfortunately, for their O-linked counterparts, no such enzyme exists. Furthermore, O-glycan heterogeneity is vast due to the lack of a common glycan core, making analysis challenging. As such, chemical methods are used to liberate O-glycans, however, often to the detriment of the glycan's structure due to "peeling" reactions. This review outlines approaches for O-glycan release and downstream glycomic and glycoproteomic analysis.
['Wilkinson|Hayden|H|0000-0002-9714-4339', 'Saldova|Radka|R|0000-0001-5085-5080']
D006031:Glycosylation D013058:Mass Spectrometry D011134:Polysaccharides D011506:Proteins
methods O-glycans glycoproteomics LC MS O-GlcNAc exoglycosidases glycosyltransferases quantitation β-elimination