Long noncoding RNA FGD5-AS1 promotes colorectal cancer cell proliferation, migration, and invasion through upregulating CDCA7 via sponging miR-302e.
The biologic function as well as the mechanism of long noncoding RNAs (lncRNAs) in colorectal cancer (CRC) still remain largely unknown. Long noncoding RNA FGD5 antisense RNA 1 (FGD5-AS1) has been reported to have a promotive effect on other human cancers, but its function in CRC still remains unknown. The expression levels of long noncoding RNA FGD5-AS1, CDCA7 mRNA, and miR-302e were assessed by RT-qPCR. The protein levels of CDCA7 were assessed by Western blot. The function of FGD5-AS1 was detected using cell viability assay, 5-ethynyl-2'-deoxyuridine (EdU) assay, transwell, and caspase-3 activity assay. Additionally, the microRNAs (miRNAs) sponge potential of FGD5-AS1 was examined by RNA immunoprecipitation assay, RNA pull-down assay, and luciferase reporter assay. FGD5-AS1 was increased in colorectal cancer cell lines compared to normal cell lines. Inhibition of FGD5-AS1 suppressed cell proliferation, migration, invasion, and accelerated cell apoptosis in CRC. FGD5-AS1 competitively bound with miR-302e to modulate CDCA7. The inhibiting effects of FGD5-AS1 knockdown on CRC cell proliferation, migration, and invasion, and the promoting effects on CRC cell apoptosis could be revived by miR-302e suppression or CDCA7 upregulation. LncRNA FGD5-AS1 could promote CRC progression through sponging miR-302e and upregulating CDCA7. FGD5-AS1 might serve as a potential therapeutic target for CRC.
['Li|Ding|D|', 'Jiang|Xiaohui|X|', 'Zhang|Xueliang|X|', 'Cao|Guangxin|G|', 'Wang|Ding|D|', 'Chen|Zhong|Z|http://orcid.org/0000-0002-5155-2038']
D017209:Apoptosis D001483:Base Sequence D001667:Binding, Competitive D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D015179:Colorectal Neoplasms D015972:Gene Expression Regulation, Neoplastic D006801:Humans D035683:MicroRNAs D009361:Neoplasm Invasiveness D009687:Nuclear Proteins D062085:RNA, Long Noncoding D015854:Up-Regulation
FGD5-AS1 CDCA7 miR-302e CRC