Exploration of Bcl-2 family and caspases-dependent apoptotic signaling pathway in Zearalenone-treated mouse endometrial stromal cells.
Zearalenone (ZEA) is a nonsteroidal estrogenic mycotoxin found in several food commodities worldwide. Although the toxicity of ZEA have been widely studied in a number of cell types, the mechanistic role of ZEA on apoptosis of endometrial stromal cells (ESCs) remains poorly understood. The objective of this study was to determine the effects of ZEA on apoptosis of mouse ESCs and explore the signaling pathway underlying the cytotoxicity of ZEA. The results showed that ZEA treatment caused obvious apoptosis in ESCs as determined by the flow cytometry and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay. Immunoblotting and real-time quantitative polymerase chain reaction (RT-qPCR) revealed that ZEA treatment increased the ratio of Bax/Bcl-2. The enzymatic activity assays revealed that caspases-3 and caspase-9 were activated by ZEA treatment in a dose-dependent manner. In addition, flow cytometry show that the apoptotic percentages of cells pretreated with Z-VAD-FMK and Z-LEHD-FMK were markedly reduced compared to the ZEA-treated cells. Overall, the results suggested that ZEA induced obvious apoptosis in ESCs via a Bcl-2 family and caspases-dependent signaling pathway.
['Hu|Jin|J|', 'Xu|Minglong|M|', 'Dai|Yujian|Y|', 'Ding|Xiaolin|X|', 'Xiao|Cheng|C|', 'Ji|Hongjun|H|', 'Xu|Yinxue|Y|']
D000590:Amino Acid Chloromethyl Ketones D000818:Animals D017209:Apoptosis D053148:Caspase 3 D053453:Caspase 9 D061945:Caspase Inhibitors D020169:Caspases D002478:Cells, Cultured D004717:Endometrium D004968:Estrogens, Non-Steroidal D005260:Female D051379:Mice D009842:Oligopeptides D019253:Proto-Oncogene Proteins c-bcl-2 D015398:Signal Transduction D017154:Stromal Cells D015025:Zearalenone D051028:bcl-2-Associated X Protein
Signaling pathway Mouse Endometrial stromal cells ZEA Apoptosis Cytotoxicity