AKT Axis, miR-21, and RECK Play Pivotal Roles in Dihydroartemisinin Killing Malignant Glioma Cells.
Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, is known to play important roles in inhibiting proliferation rate, inducing apoptosis, as well as hindering the metastasis and invasion of glioma cells, but the underlying mechanisms are still unclear so far. In this study, methyl thiazolyl tetrazolium (MTT), colony-forming, wound healing, invasion, and apoptosis assays were performed to investigate the effect of DHA on malignant glioma cells. Results showed that DHA induced apoptosis of malignant glioma cells through Protein Kinase B (AKT) axis, induced death of malignant glioma cells by downregulating miR-21, and inhibited the invasion of malignant glioma cells corresponding with up-regulation of the reversion-inducing-cysteine-rich protein with kazal motifs (RECK). These results revealed that AKT axis, miR-21, and RECK play pivotal roles in DHA killing malignant glioma cells, suggesting that DHA is a potential agent for treating glioma.
['Shao|Ying-Ying|YY|', 'Zhang|Tao-Lan|TL|', 'Wu|Lan-Xiang|LX|', 'Zou|He-Cun|HC|', 'Li|Shuang|S|', 'Huang|Jin|J|', 'Zhou|Hong-Hao|HH|']
D000328:Adult D000368:Aged D017209:Apoptosis D037621:Artemisinins D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D005260:Female D058851:GPI-Linked Proteins D015972:Gene Expression Regulation, Neoplastic D005910:Glioma D006801:Humans D008297:Male D035683:MicroRNAs D008875:Middle Aged D009367:Neoplasm Staging D051057:Proto-Oncogene Proteins c-akt
miR-21 RECK glioma dihydroartemisinin (DHA) apoptosis metastasis invasion