Electrospun poly(hydroxybutyrate) scaffolds promote engraftment of human skin equivalents via macrophage M2 polarization and angiogenesis.
Human dermo-epidermal skin equivalents (DE) comprising in vitro expanded autologous keratinocytes and fibroblasts are a good option for massive burn treatment. However, the lengthy expansion time required to obtain sufficient surface to cover an extensive burn together with the challenging surgical procedure limits their clinical use. The integration of DE and biodegradable scaffolds has been proposed in an effort to enhance their mechanical properties. Here, it is shown that poly(hydroxybutyrate) electrospun scaffolds (PHB) present good biocompatibility both in vitro and in vivo and are superior to poly-ε-caprolactone electrospun scaffolds as a substrate for skin reconstruction. Implantation of PHB scaffolds in healthy rats polarized macrophages to an M2-type that promoted constructive in vivo remodelling. Moreover, implantation of DE-PHB composites in a NOD/SCID mouse xenograft model resulted in engraftment accompanied by an increase in angiogenesis that favoured the survival of the human graft. Thus, PHB scaffolds are an attractive substrate for further exploration in skin reconstruction procedures, probably due in part to their greater angiogenic and M2 macrophage polarization properties. Copyright © 2017 John Wiley & Sons, Ltd.
['Castellano|Delia|D|', 'Sanchis|Ana|A|', 'Blanes|María|M|', 'Pérez Del Caz|Mª Dolores|MD|', 'Ruiz-Saurí|Amparo|A|', 'Piquer-Gil|Marina|M|', 'Pelacho|Beatriz|B|', 'Marco|Bruno|B|', 'Garcia|Nahuel|N|', 'Ontoria-Oviedo|Imelda|I|', 'Cambra|Vicente|V|', 'Prosper|Felipe|F|', 'Sepúlveda|Pilar|P|0000-0001-6071-7431']
D000818:Animals D001672:Biocompatible Materials D002454:Cell Differentiation D016764:Cell Polarity D049109:Cell Proliferation D020405:Dermis D004817:Epidermis D061307:Human Umbilical Vein Endothelial Cells D006801:Humans D006885:Hydroxybutyrates D015603:Keratinocytes D008264:Macrophages D008297:Male D016688:Mice, Inbred NOD D016513:Mice, SCID D018919:Neovascularization, Physiologic D011108:Polymers D000091402:Prohibitins D017208:Rats, Wistar D016038:Skin Transplantation D017768:Skin, Artificial D023822:Tissue Engineering D054457:Tissue Scaffolds
poly(hydroxybutyrate) skin equivalents electrospinning human skin xenograft