Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods.
In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.
['Yamamoto|Naoki|N|', 'Kato|Yoshinao|Y|', 'Sato|Atsushi|A|', 'Hiramatsu|Noriko|N|', 'Yamashita|Hiromi|H|', 'Ohkuma|Mahito|M|', 'Miyachi|Ei-Ichi|E|', 'Horiguchi|Masayuki|M|', 'Hirano|Koji|K|', 'Kojima|Hajime|H|']
D000818:Animals D002453:Cell Cycle D002460:Cell Line D003315:Cornea D050759:Cyclin-Dependent Kinase Inhibitor p21 D004847:Epithelial Cells D019573:Epithelium, Corneal D006801:Humans D066298:In Vitro Techniques D011817:Rabbits
Eye irritation Corneal epithelium CDKN1A In vitro model SIRC cell