Cloning, Expression, and Bioinformatics Analysis of Heavy Metal Resistance Gene afe_1862 from Acidithiobacillus ferrooxidans L1 in Escherichia coli.
Molecular studies of copper and cadmium resistances in acidophilic bacteria are significant in biomining. In this study, afe_1862, which encodes a heavy metal-binding protein in Acidithiobacillus ferrooxidans L1, was amplified using PCR, cloned into the pET32a plasmid, and sequenced. Following SDS-PAGE analysis, optimization of the expression conditions and heterologous overexpression of afe_1862 in Escherichia coli BL21 in the presence of Cu2+ and Cd2+ were studied as well. The results indicated that AFE_1862 has higher resistance to Cu2+ than Cd2+. Bioinformatics analysis illustrated that AFE_1862 has a conserved HMA domain containing heavy metal-binding sites, which may play a role in transporting or detoxifying heavy metals.
['Leng|Feifan|F|', 'Li|Yuanli|Y|', 'Luo|Wen|W|', 'Wei|Qingwei|Q|', 'Jing|Yanjun|Y|', 'Wang|Xiaoli|X|', 'Yang|Mingjun|M|', 'Wang|Yonggang|Y|http://orcid.org/0000-0003-3762-6421']
D042763:Acidithiobacillus D001426:Bacterial Proteins D019295:Computational Biology D004926:Escherichia coli D019216:Metals, Heavy
Expression Bioinformatics Metal resistance afe_1862 Acidithiobacillus ferrooxidans