Neuroprotection by ethanolic extract of Syzygium aromaticum in Alzheimer's disease like pathology via maintaining oxidative balance through SIRT1 pathway.
The oxidative stress plays a key role in Alzheimer's disease (AD) and Sirtuin (SIRT1) is potential mediator of oxidative pathway. This study explored the role of Syzygium aromaticum on SIRT1 and oxidative balance in amyloid beta induced toxicity. Anti-oxidative capacity of Syzygium aromaticum was performed in Aβ25-35 induced neurotoxicity in neuronal cells. Superoxide dismutase, Catalase and Glutathione enzyme activity were determined by the treatment of Syzygium aromaticum. Both recombinant and endogenous SIRT1 activity were performed in its presence. The expression of γ-secretase and SIRT1 were evaluated by western blot. Syzygium aromaticum was capable to scavenge ROS and elevate the percentage of anti-oxidant enzymes. It also activated and elevated the level of SIRT1 and downregulated γ-secretase level. These findings show a holistic approach towards the neurodegenerative disease management by Syzygium aromaticum which could lead to the formulation of new drug for AD. This Ayurvedic product can give a healthy aging with no side effects and also be cost effectives. It may meet unmet medical needs of current relevance.
['Shekhar|Shashank|S|', 'Yadav|Yudhishthir|Y|', 'Singh|Amrendra Pratap|AP|', 'Pradhan|Rashmita|R|', 'Desai|Gaurav Rajesh|GR|', 'Dey|A B|AB|', 'Dey|Sharmistha|S|']
D000544:Alzheimer Disease D053829:Amyloid Precursor Protein Secretases D002374:Catalase D045744:Cell Line, Tumor D005978:Glutathione D057809:HEK293 Cells D006801:Humans D000066829:Neuroprotection D018384:Oxidative Stress D010936:Plant Extracts D056564:Sirtuin 1 D013482:Superoxide Dismutase D027842:Syzygium
Syzygium aromaticum Alzheimer's disease SIRT1 SH-SY5Y Serum