Amphiphilic Poly(N-vinylpyrrolidone) Nanoparticles Conjugated with DR5-Specific Antitumor Cytokine DR5-B for Targeted Delivery to Cancer Cells.
Nanoparticles based on the biocompatible amphiphilic poly(N-vinylpyrrolidone) (Amph-PVP) derivatives are promising for drug delivery. Amph-PVPs self-aggregate in aqueous solutions with the formation of micellar nanoscaled structures. Amph-PVP nanoparticles are able to immobilize therapeutic molecules under mild conditions. As is well known, many efforts have been made to exploit the DR5-dependent apoptosis induction for cancer treatment. The aim of the study was to fabricate Amph-PVP-based nanoparticles covalently conjugated with antitumor DR5-specific TRAIL (Tumor necrosis factor-related apoptosis-inducing ligand) variant DR5-B and to evaluate their in vitro cytotoxicity in 3D tumor spheroids. The Amph-PVP nanoparticles were obtained from a 1:1 mixture of unmodified and maleimide-modified polymeric chains, while DR5-B protein was modified by cysteine residue at the N-end for covalent conjugation with Amph-PVP. The nanoparticles were found to enhance cytotoxicity effects compared to those of free DR5-B in both 2D (monolayer culture) and 3D (tumor spheroids) in vitro models. The cytotoxicity of the nanoparticles was investigated in human cell lines, namely breast adenocarcinoma MCF-7 and colorectal carcinomas HCT116 and HT29. Notably, DR5-B conjugation with Amph-PVP nanoparticles sensitized resistant multicellular tumor spheroids from MCF-7 and HT29 cells. Taking into account the nanoparticles loading ability with a wide range of low-molecular-weight antitumor chemotherapeutics into hydrophobic core and feasibility of conjugation with hydrophilic therapeutic molecules by click chemistry, we suggest further development to obtain a versatile system for targeted drug delivery into tumor cells.
['Yagolovich|Anne|A|0000-0003-3145-3726', 'Kuskov|Andrey|A|0000-0001-8140-2754', 'Kulikov|Pavel|P|0000-0001-5221-7613', 'Kurbanova|Leily|L|', 'Bagrov|Dmitry|D|', 'Artykov|Artem|A|0000-0003-2585-9631', 'Gasparian|Marine|M|', 'Sizova|Svetlana|S|', 'Oleinikov|Vladimir|V|', 'Gileva|Anastasia|A|', 'Kirpichnikov|Mikhail|M|', 'Dolgikh|Dmitry|D|0000-0002-9880-7202', 'Markvicheva|Elena|E|']
poly(N-vinylpyrrolidone) nanoparticles multicellular tumor spheroids DR5 receptor TRAIL variant DR5-B antitumor therapy self-assembly targeted drug delivery system