Skin microbiome in melanomas and melanocytic nevi.
BACKGROUND
High-throughput DNA sequencing has shown that the cutaneous microbiome varies due to different exogenous and endogenous factors.


OBJECTIVES
To characterize the microbiome of cutaneous melanomas and melanocytic nevi.


MATERIAL AND METHODS
Non-invasive swab specimens were taken from 15 cutaneous melanomas and 17 benign melanocytic nevi. Partial sequencing of the 16S ribosomal RNA gene was carried out on the 454 GS-FLX Titanium platform and the resulting sequence data was analysed by bioinformatics and statistical methods. 95% of the OTUs (Operational Taxonomic Units) belonged to four phyla: Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes. The genus Propionibacterium was overall the most common genus, followed by Staphylococcus and Corynebacterium. Statistical analysis showed no significant differences in the relative abundances of bacterial genera or bacterial diversity between the patient groups. Melanoma samples showed a marginally decreased cutaneous microbial diversity.


CONCLUSION
Our data suggests that the skin microbiome may not be a useful diagnostic tool for melanoma and melanocytic nevi.
['Salava|Alexander|A|', 'Aho|Velma|V|', 'Pereira|Pedro|P|', 'Koskinen|Kaisa|K|', 'Paulin|Lars|L|', 'Auvinen|Petri|P|', 'Lauerma|Antti|A|']
D000368:Aged D005260:Female D006801:Humans D008297:Male D008545:Melanoma D064307:Microbiota D008875:Middle Aged D009508:Nevus, Pigmented D012867:Skin D012878:Skin Neoplasms
Skin microbiome melanoma benign melanocytic nevi cutaneous microbial diversity