Immunoglobulin E and immunoglobulin G cross-reactive allergens and epitopes between cow milk αS1-casein and soybean proteins.
Some infants allergic to cow milk-based formula are also sensitive to soybean-based formula. This paper aimed to explore the association of IgE and IgG cross-reactivity between αS1-casein in cow milk (CM) and soybean proteins. The IgE and IgG cross-reactive allergens and epitopes were identified using sera from infants allergic to CM or mice monoclonal antibodies. The AA sequence alignment was performed using bioinformatics software. Finally, the digestion and heating stability of the cross-reactive allergen were explored by sodium dodecyl sulfate (SDS)-PAGE and Western blotting. The results showed that the IgE and IgG cross-reactive allergen was α subunit of β-conglycinin named Gly m Bd 60K. The IgE and IgG epitopes were the sequences at AA 319-341 and AA 164-182. No intact Gly m Bd 60K allergen could be observed after 2 min in simulated gastric fluid by SDS-PAGE. Heating did not change IgE and IgG cross-reactivity by Western blotting. Therefore, the existence of cross-reactivity between CM αS1-casein and soybean proteins possibly contributes to the frequently observed cosensitization for these allergens in cow milk-allergic patients. The same IgE- and IgG-binding epitopes of cross-reactive allergens may provide important information for elucidation of the association between IgG and IgE antibody generation.
['Cong|Yanjun|Y|', 'Li|Ye|Y|', 'Li|Linfeng|L|']
D000485:Allergens D000818:Animals D052179:Antigens, Plant D015153:Blotting, Western D002364:Caseins D002648:Child D002675:Child, Preschool D003429:Cross Reactions D000939:Epitopes D005916:Globulins D006801:Humans D007073:Immunoglobulin E D007074:Immunoglobulin G D007223:Infant D051379:Mice D008807:Mice, Inbred BALB C D008892:Milk D016269:Milk Hypersensitivity D055314:Seed Storage Proteins D030262:Soybean Proteins D013025:Soybeans
cross-reactivity epitope soybean protein α(S1)-casein