Effect of Bovine Serum Albumin Supplementation in Tris-Soybean Lecithin-Based Extender on Quality of Chilled Ram Epididymal Spermatozoa.
This study was designed to assess the effects of using tris-soybean lecithin (TSL)-based extender supplemented with bovine serum albumin (BSA) on the quality of ram epididymal spermatozoa during refrigerated storage.  Epididymal sperm were collected from 22 Zandi rams, diluted in TSL-based extender at different concentrations (0%, 2.5%, 5%, 7.5%, and 10%) of BSA, and stored for 5 days at 4°C. Sperm parameters including motility, viability, plasma membrane integrity, chromatin protamination, and malondialdehyde (MDA) content were evaluated at 0, 24, 72, and 120 hours of refrigeration.  The addition of 10% BSA to the extender significantly improved sperm viability at 24 and 120 hours of refrigerated liquid storage (p < 0.05). An enhancement in plasma membrane integrity was observed along with a decrease in MDA level by increasing the concentration of BSA from 0% to 10% (p > 0.05). Sperm motion characteristics were higher in the BSA-free group at 120 hours of preservation (p < 0.05). No statistical difference was found for nuclear protamination between experimental groups (p > 0.05).  BSA supplementation in TSL-based extender can preserve the viability of epididymal ram spermatozoa during liquid storage at 4°C.
['Rahimizadeh|Pegah|P|', 'Rezaei Topraggaleh|Tohid|T|', 'Bucak|Mustafa Numan|MN|', 'Ziarati|Niloofar|N|', 'Hasirbaf|Arash|A|', 'Taher-Mofrad|Seyed Mohamad Javad|SMJ|', 'Maroufizadeh|Saman|S|', 'Shahverdi|Abdolhossein|A|']
D015925:Cryopreservation D019587:Dietary Supplements D006801:Humans D054709:Lecithins D008297:Male D012662:Semen Preservation D012710:Serum Albumin, Bovine D013025:Soybeans D013081:Sperm Motility D013094:Spermatozoa
ram BSA epididymal sperm liquid preservation soybean lecithin