Anti-cervical cancer activity of secondary metabolites of endophytic fungi from Ginkgo biloba.
OBJECTIVES
The purpose of this study was to isolate the secondary metabolites of endophytic fungi from Ginkgo biloba (SMEFGB) and investigate their anti-cervical cancer activity.


METHODS
SMEFGB were cultured. The secondary metabolites of endophytic fungi was extracted, purified and identified. The effects of secondary metabolites on proliferation, apoptosis and migration of human cervical cancer HeLa cells were determined. In addition, the effects of SMEFGB on growth of Hela implanted tumor in mice were investigated.


RESULTS
In 9 stains of endophytic fungi successfully isolated from the leaves of Ginkgo biloba, the stain J-1, J-2 and J-3 could produce podophyllotoxin. These 3 stains were identified by molecular biology. The secondary metabolites of stain J-1, J-2 and J-3 markedly inhibited the proliferation of HeLa cells, promoted their apoptosis and blocked their migration. In addition, the secondary metabolites of stain J-1, J-2 and J-3 significantly attenuated the growth of HeLa implanted tumor in mice.


CONCLUSIONS
Our results indicated that SMEFGB had obvious anti-cervical cancer activity in vitro and in vivo.
['He|Qing|Q|', 'Zeng|Qiangcheng|Q|', 'Shao|Yibo|Y|', 'Zhou|Haixia|H|', 'Li|Tianjiao|T|', 'Song|Fang|F|', 'Liu|Wei|W|']
D000818:Animals D000970:Antineoplastic Agents D017209:Apoptosis D001688:Biological Products D002465:Cell Movement D049109:Cell Proliferation D060026:Endophytes D005260:Female D005658:Fungi D020441:Ginkgo biloba D006367:HeLa Cells D006801:Humans D051379:Mice D064210:Secondary Metabolism D002583:Uterine Cervical Neoplasms D023041:Xenograft Model Antitumor Assays
anti-cervical cancer activity Secondary metabolites endophytic fungi Ginkgo biloba cervical cancer