Plasma long noncoding RNA IL-7R as a prognostic biomarker for clinical outcomes in patients with acute respiratory distress syndrome.
BACKGROUND
Long non-coding RNAs (lncRNAs) regulate a variety of genes and biological processes. Lnc-IL7R plays a considerable role in the regulation of inflammation, but its prognostic potential in acute respiratory distress syndrome (ARDS) has not been fully explained. In this study, the role of lnc-IL7R as a potential biomarker in ARDS was examined.


OBJECTIVE
Role of lnc-IL7R as potential biomarker in ARDS.


METHODS
LncRNA-IL7R was isolated from the plasma of patients with ARDS and healthy controls and clinical indexes were obtained within 24 h after admission. The relative expression of lnc-IL7R was obtained by quantitative real-time PCR. The correlations between lnc-IL7R and continuous variables in ARDS were tested using Spearman's coefficients.


RESULTS
A total of 85 ARDS patients and 49 healthy controls were included. Plasma lnc-IL7R was significantly down-regulated in ARDS compared with the levels in healthy control individuals, especially in severe ARDS (P < .01). The area under the curve (AUC) of lnc-IL7R for ARDS diagnosis was 0.87 (sensitivity 75.3%, specificity 93.9%). The lnc-IL7R levels were correlated with the severity of ARDS (ρ = -0.31, P = .0215), oxygenation index (ρ = 0.61, P < .001), APACHE II score (ρ = -0.04, P = .0230), CRP (ρ = -0.26, P = .0148) and WBC (ρ = -0.29, P = .0064). Lnc-IL7R relative value ≥ 0.33 showed the lower 28-day mortality in the patients with ARDS(P < .05).The survivors showed higher lnc-IL7R level and lower APACHE II score, SOFA score and length of mechanical ventilation than in the non-survivors (P = .0109, P < .001, P < .001 and P = .017, respectively).


CONCLUSIONS
Lnc-IL7R is a novel biomarker for the diagnosis of ARDS and predicts the severity of ARDS and 28-day mortality in this patients cohort.


TRIAL REGISTRATION
The study was registered with the Chinese Clinical Trial Registry (ChiCTR-DOD-16008657).
['Wan|Bing|B|http://orcid.org/0000-0002-8370-2977', 'Xu|Wu-Jian|WJ|', 'Xu|Wei-Nan|WN|', 'Zhan|Ping|P|', 'Wu|Guan-Nan|GN|', 'Jin|Jia-Jia|JJ|', 'Xi|Guang-Min|GM|', 'Yin|Jiang-Tao|JT|', 'Zhang|Hao|H|', 'Chen|Yi-Kun|YK|', 'Miao|Ying-Ying|YY|', 'Wang|Xiao-Xia|XX|', 'Xu|Ying-Kun|YK|', 'Lv|Tang-Feng|TF|', 'Song|Yong|Y|']
D015415:Biomarkers D005260:Female D005500:Follow-Up Studies D005786:Gene Expression Regulation D006801:Humans D008297:Male D008875:Middle Aged D011379:Prognosis D011446:Prospective Studies D062085:RNA, Long Noncoding D012372:ROC Curve D060888:Real-Time Polymerase Chain Reaction D020395:Receptors, Interleukin-7 D012121:Respiration, Artificial D012128:Respiratory Distress Syndrome
biomarker ARDS diagnosis oxygenation index LncIL7R prognosis