Phytochemical analysis with the antioxidant and aldose reductase inhibitory capacities of Tephrosia humilis aerial parts' extracts.
The aerial parts of Tephrosia humilis were tested about their antioxidant potential, their ability to inhibit the aldose/aldehyde reductase enzymes and their phenolic content. The plant material was exhaustively extracted with petroleum ether, dichloromethane and methanol, consecutively. The concentrated methanol extract was re-extracted, successively, with diethyl ether, ethyl acetate and n-butanol. All extracts showed significant antioxidant capacity, but the most effective was the ethyl acetate extract. As about the aldose reductase inhibition, all fractions, except the aqueous, were strong inhibitors of the enzyme, with the n-butanolic and ethyl acetate fractions to inhibit the enzyme above 75%. These findings provide support to the ethnopharmacological usage of the plant as antioxidant and validate its potential to act against the long-term diabetic complications. The phytochemical analysis showed the presence of 1,4-dihydroxy-3,4-(epoxyethano)-5-cyclohexene(1), cleroindicin E(2), lupeol(3), methyl p-coumarate(4), methyl 4-hydroxybenzoate(5), prunin(6), 5,7,2',5'-tetrahydroxyflavanone 7-rutinoside(7), protocatechuic acid(8), luteolin 7-glucoside(9), apigenin(10), naringin(11), rhoifolin(12) and luteolin 7-glucuronate(13).
['Plioukas|Michael|M|', 'Gabrieli|Chrysi|C|', 'Lazari|Diamanto|D|', 'Kokkalou|Eugene|E|']
D000449:Aldehyde Reductase D000975:Antioxidants D004187:Disaccharides D004353:Drug Evaluation, Preclinical D004791:Enzyme Inhibitors D020834:Ethnopharmacology D005419:Flavonoids D005960:Glucosides D006027:Glycosides D020128:Inhibitory Concentration 50 D047311:Luteolin D010226:Parabens D010636:Phenols D035261:Plant Components, Aerial D010936:Plant Extracts D031304:Tephrosia
aldose reductase Tephrosia DPPH antioxidant activity lipoxygenase luminol chemiluminescence