Vaccination with rEGVac elicits immunoprotection against different stages of Echinococcus granulosus life cycle: A pilot study.
Vaccination against dog-sheep transmission cycle is necessary to control cystic echinococcosis (CE) infection. A multi-epitope multi-antigenic recombinant vaccine was developed-comprising the three putative vaccine antigens EG95, Eg14-3-3 and EgEnolase-was cloned and expressed. In a pilot experiment, the multi-antigen vaccine was assessed in 15 dogs and 15 sheep (five experimental groups and three animals in each group) by two subcutaneous doses 28 days apart. To evaluate the efficacy of the vaccine candidate first immunological analysis were done comprising IgG and IgE antibodies and the cytokine IL-4 in sera of the immunized dogs and sheep. Serum IgG, IgE, and IL-4, in particular in the dogs, were increased after the two rounds of vaccine candidate injection, while the total number of hydatid cysts was reduced (~85.43%). This pilot trial indicated significant immune protection efficacy against E. granulosus especially in dogs, while its efficacy in sheep was not as high as dogs. The multi-antigenic candidate vaccine is proposed as a protective vaccine modality in both dogs and sheep.
['Pourseif|Mohammad M|MM|', 'Moghaddam|Gholamali|G|', 'Nematollahi|Ahmad|A|', 'Khordadmehr|Monireh|M|', 'Naghili|Behrouz|B|', 'Dehghani|Jaber|J|', 'Omidi|Yadollah|Y|']
D000818:Animals D000947:Antigens, Helminth D016207:Cytokines D004285:Dogs D004443:Echinococcosis D048209:Echinococcus granulosus D008018:Life Cycle Stages D010865:Pilot Projects D012756:Sheep D012757:Sheep Diseases D014611:Vaccination D014614:Vaccines, Synthetic
Vaccine Immunoprotection Echinococcus granulosus Antigen Epitope Freund's adjuvant