The role of SPRASA in female fertility.
Fertility is a complex process and infertility can have many causes. Sperm protein reactive with antisperm antibody (SPRASA)/sperm lysozyme-like protein 1 is a protein discovered as the target of autoantibodies in infertile men and previously thought to be expressed only in sperm. Using a bovine in vitro fertilization model, we have shown that SPRASA antiserum reduced sperm binding to zona-free oocytes and the development of embryos to morulae but did not affect the postfertilization cleavage rate to 2 cells or sperm motility. We demonstrated that SPRASA was expressed in ovarian follicles, corpora lutea, and oocytes by a combination of reverse transcription-polymerase chain reaction and immunohistochemistry. Female mice immunized with SPRASA had profound infertility following timed matings and those mice that did become pregnant had reduced fetal viability. The levels of antibodies reactive with SPRASA in 204 fertile and 202 infertile couples were elevated in 3 infertile but no fertile women. Together, these results indicate that SPRASA has a role in female fertility.
['Wagner|Angela|A|', 'Holland|Olivia J|OJ|', 'Tong|Mancy|M|', 'Shelling|Andrew N|AN|', 'Chamley|Lawrence W|LW|']
D000818:Animals D001323:Autoantibodies D016022:Case-Control Studies D002415:Cats D002417:Cattle D002970:Cleavage Stage, Ovum D003338:Corpus Luteum D004195:Disease Models, Animal D004285:Dogs D046149:Embryo Culture Techniques D047108:Embryonic Development D005260:Female D005298:Fertility D005307:Fertilization in Vitro D006801:Humans D007247:Infertility, Female D007519:Isoantigens D008297:Male D051379:Mice D009865:Oocytes D006080:Ovarian Follicle D011247:Pregnancy D029589:Seminal Plasma Proteins D013084:Sperm-Ovum Interactions
SPRASA infertility oocyte SLLP1 SPACA3 contraceptive