Protective effect of picroside II on myocardial ischemia reperfusion injury in rats.
The aim of this study was to determine the effect of picroside II on myocardial ischemia reperfusion injury in rats and to explore its underlying mechanism. Isolated rat hearts underwent 30 minutes of global ischemia followed by 120 minutes of reperfusion. Different doses of picroside II (1 μM, 10 μM, and 100 μM) were given 20 minutes before ischemia. Phosphoinositide 3-kinase inhibitor (wortmannin) and nitric oxide synthase (NOS) inhibitor (L-N(G)-nitroarginine methyl ester) were given 10 minutes before picroside II treatment. The cardiac function, myocardial infarct size, apoptosis, myocardial nitric oxide content, the expressions of Bcl-2 and Bax, and the activation of the phosphoinositide 3-kinase/Akt/endothelial NOS pathway were evaluated. Treatment with 10 μM and 100 μM picroside II significantly improved postischemic myocardial function, reduced myocardial infarct size, inhibited apoptosis, increased myocardial NO content, upregulated Bcl-2, downregulated Bax, and increased the phosphorylation of Akt and endothelial NOS, but cardioprotection was not shown in the 1 μM picroside II treatment group and was abrogated by wortmannin and L-N(G)-nitroarginine methyl ester. Furthermore, cardioprotection in the 100 μM picroside II treatment group was superior to that in the 10 μM picroside II treatment group. In conclusion, the data reveals that picroside II has a significant protective effect on myocardial ischemia reperfusion injury in a dose-dependent manner, which was mediated by upregulating the phosphoinositide 3-kinase/Akt/endothelial NOS pathway to increase nitric oxide production and regulating the expressions of Bcl-2 and Bax to inhibit apoptosis.
['Wu|Nan|N|', 'Li|Wenna|W|', 'Shu|Wenqi|W|', 'Jia|Dalin|D|']
D000730:Androstadienes D000818:Animals D017209:Apoptosis D002934:Cinnamates D006339:Heart Rate D057888:Iridoid Glucosides D008297:Male D015428:Myocardial Reperfusion Injury D019331:NG-Nitroarginine Methyl Ester D009569:Nitric Oxide D051057:Proto-Oncogene Proteins c-akt D019253:Proto-Oncogene Proteins c-bcl-2 D051381:Rats D017208:Rats, Wistar D016277:Ventricular Function, Left D000077191:Wortmannin D051028:bcl-2-Associated X Protein
picroside II ischemia reperfusion injury nitric oxide apoptosis endothelial nitric oxide synthase