The Histochemical Alterations of Mucin in Colorectal Carcinoma Quantified by Two Efficient Algorithms of Digital Image Analysis.
The practical use of knowledge on the diagnostic-prognostic role of polysaccharide components of mucins in colorectal cancer (CRC) has been difficult, due to the number of histochemical (HC) reaction types, as well as lack of standard methods of computer-assisted analysis of tissue expression of these molecules. Using two algorithms of digital image analysis (by application of Image-Pro Premier and our originally designed program Filter HSV), we evaluated the expression of polysaccharides in tissue samples of CRC patients (n = 33), and fragments of normal colorectal tissue from the same patients (control) using periodic acid Schiff reaction (PAS) (neutral mucins) and alcian blue staining (AB) (acidic mucins). Our results indicate lower expression of the PAS+ and AB+ mucins in CRC, as compared to the control samples. The higher expression of PAS+ polysaccharides was detected in flat tumors than in protruded CRC, while higher AB+ mucins expression was a feature of mucinous CRC subtypes. Positive correlation between mutual PAS+ and AB+ expression, as well as correlations with glucose concentration (PAS+ mucins), and hemoglobin level (AB+ mucins) were observed exclusively in unchanged colorectal samples (control). Both algorithms of digital image analysis (smart segmentation and Filter HSV) work properly and can be used interchangeably in daily practice of pathologists, as useful tools of quantitative evaluation of HC reaction in both normal and cancerous tissues.
['Kasprzak|Aldona|A|', 'Seraszek-Jaros|Agnieszka|A|', 'Jagielska|Joanna|J|', 'Helak-Łapaj|Celina|C|', 'Siodła|Elżbieta|E|', 'Szmeja|Jacek|J|', 'Kaczmarek|Elżbieta|E|']
D000328:Adult D000368:Aged D000465:Algorithms D015415:Biomarkers D015179:Colorectal Neoplasms D000078332:Data Analysis D005260:Female D006651:Histocytochemistry D006801:Humans D007091:Image Processing, Computer-Assisted D053208:Kaplan-Meier Estimate D008297:Male D008875:Middle Aged D057054:Molecular Imaging D009077:Mucins D060787:Neoplasm Grading D009367:Neoplasm Staging D011379:Prognosis
digital image analysis colorectal cancer PAS and alcian blue histochemistry mucin polysaccharides