Toxoplasma invasion delayed by TgERK7 eradication.
Toxoplasma gondii causes serious clinical toxoplasmosis in humans mostly due to its asexual life cycles, which can be artificially divided into five tightly coterminous stages. Any radical or delay for the stage will result in tremendous changes immediately behind. We previously demonstrated that TgERK7 is associated with the intracellular proliferation of T. gondii, but during the process, other stages before were not meanwhile determined. To further clarify the function of ERK7 gene in T. gondii, the complemental strain of ΔTgERK7 tachyzoites created previously was engineered via electric transfection with the recombinant pUC/Tgerk7 plasmid, named pUC/TgERK7 strain in this study, and was used together with ΔTgERK7 and wild-type GT1 strains to retrospect the phenotypic changes including invasion and attachment. The results showed that TgERK7 protein can be re-expressed in the ΔTgERK7 tachyzoites and eradication of this protein leads to significantly lower invasion of T. gondii at 1 h and 2 h post-infection (P < 0.05), which is the key factor causing the following slow intracellular proliferation, in comparison with wild-type GT1 and pUC/TgERK7 parasites; noteworthily, at other early time points including 15 min for attachment assay was no statistical difference (P > 0.05). The data suggested that ERK7 protein in T. gondii is an important virulence factor that participates in the invasion of this parasite.
['Li|Zhong-Yuan|ZY|http://orcid.org/0000-0001-7273-6189', 'Liang|Xue|X|', 'Guo|Hai-Ting|HT|', 'Tan|Jie|J|', 'Zhu|Xing-Quan|XQ|', 'Liu|Quan|Q|']
D000818:Animals D048049:Extracellular Signal-Regulated MAP Kinases D005816:Genetic Complementation Test D006801:Humans D008018:Life Cycle Stages D009154:Mutation D015800:Protozoan Proteins D014122:Toxoplasma D014123:Toxoplasmosis D014774:Virulence
Invasion TgERK7 Eradication Toxoplasma gondii Complemental expression