Bioanalytical method development and validation for determination of fibroblast growth factor peptide and its application to pharmacokinetic studies.
Fibroblast growth factor peptide (FGF-P) is a polypeptide analog of FGF-2 that could be a potential mitigation and treatment agent for radiation syndromes. Prior to conducting preclinical pharmacokinetics, we developed and validated the LC-MS/MS bioanalytical method for determination of FGF-P in rat plasma for the first time. FGF-P was extracted from rat plasma using the protein precipitation technique followed liquid-liquid extraction using dichloromethane as a solvent. The mobile phases consisted of two components: (a) 0.1% formic acid in water; and (b) acetonitrile: 0.1% formic acid in water (95:5) under gradient elution. The validated method was also successfully applied to a pharmacokinetic study of FGF-P (10 mg/kg, intravenous) in Wistar rats. The method proved to be specific, accurate, precise, and linear over the concentration range of 2-500 ng/mL with coefficient of determination greater than 0.99 in all validation batches. The within-run and between-run accuracy was 87.97-115.00% with a precision of less than 14%. The mean recoveries ranged from 88.14% to 101.73%. The stability of the compound in plasma samples was proven under various storage conditions. After intravenous administration of FGF-P (10 mg/kg) the C0 was 70.4 µg/mL and the AUC was 86.2 µg*min/mL.
['Prasanchaimontri|Ing-Orn|IO|', 'Chandasana|Hardik|H|', 'Akbar|Mohammad A|MA|', 'Swarts|Steven|S|', 'Okuneiff|Paul|P|', 'Derendorf|Hartmut|H|']
D061605:Administration, Intravenous D000818:Animals D019540:Area Under Curve D002853:Chromatography, Liquid D004355:Drug Stability D004356:Drug Storage D016222:Fibroblast Growth Factor 2 D059625:Liquid-Liquid Extraction D010446:Peptide Fragments D051381:Rats D017208:Rats, Wistar D015203:Reproducibility of Results D053719:Tandem Mass Spectrometry
Bioanalytical Fibroblast growth factor Pharmacokinetics Polypeptide LC-MS/MS