Transient Transformation Using Particle Bombardment for Gene Expression Analysis.
Transient transformation or transient expression results in rapid and fleeting gene expression. This approach is often used as a first-tier screening tool for evaluation of components that affect gene expression. Here, we describe the use of particle bombardment of lima bean cotyledons with constructs containing the green fluorescent protein (gfp) coding region for evaluation of promoter components that influence gene expression. Although this approach is conceptually quite simple, this lima bean transient expression system may not work well, if our methods and notes are not carefully read and followed. Our laboratory has successfully optimized this method over the past 10 years, resulting in a transient expression system, which works like no other that we have seen.
['Gunadi|Andika|A|', 'Dean|Eric A|EA|', 'Finer|John J|JJ|']
D018548:Cotyledon D020869:Gene Expression Profiling D018506:Gene Expression Regulation, Plant D018014:Gene Transfer Techniques D049452:Green Fluorescent Proteins D027805:Phaseolus D030821:Plants, Genetically Modified D011401:Promoter Regions, Genetic D014170:Transformation, Genetic
Transient expression Lima bean cotyledon Green fluorescent protein Gene introduction Promoter analysis