Production of bacteriocin by Virgibacillus salexigens isolated from "terasi": a traditionally fermented shrimp paste in Indonesia.
A natural antibacterial-substance-producing gram-positive bacterium was isolated from terasi shrimp paste, a popular fermented product in Indonesia. This strain, a spore-forming and strictly aerobic bacterium, was identified as Virgibacillus salexigens by 16S rRNA gene sequence analysis. The antibacterial substance purified from the precipitated product in the culture supernatant of the strain using ammonium sulfate showed a broad inhibition spectrum against gram-positive bacteria, including a typical foodborne bacterium, namely, Listeria monocytogenes. The antibacterial activity of the substance was inactivated by treatments with various proteolytic enzymes. It was stable after heating or pH treatment, and approximately 60% of the initial activity remained even after heating at 121 °C for 15 min. In addition, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis indicated that its monoisotopic mass weight was 5318.4 Da (M+H)(+). On the basis of the results obtained by the automated Edman degradation technique and MALDI-TOF MS analysis, the substance can be classified as a member of Class IId bacteriocins, but it could not be identified as any of the previously purified substances except for the putative bacteriocin predicted from the draft genome sequence data of gram-positive bacteria such as Virgibacillus and Bacillus strains.
['Kobayashi|Takeshi|T|', 'Agustini|Tri Winarni|TW|', 'Ibrahim|Ratna|R|', 'Kamei|Kaeko|K|', 'Kondo|Akihiro|A|', 'Kajiwara|Michika|M|', 'Ooka|Yoshihiro|Y|', 'Nakamura|Hidetoshi|H|', 'Terahara|Takeshi|T|', 'Imada|Chiaki|C|']
D000332:Aerobiosis D001430:Bacteriocins D004269:DNA, Bacterial D004275:DNA, Ribosomal D005516:Food Microbiology D006094:Gram-Positive Bacteria D006863:Hydrogen-Ion Concentration D007214:Indonesia D008970:Molecular Weight D059748:Proteolysis D012336:RNA, Ribosomal, 16S D017422:Sequence Analysis, DNA D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization D013171:Spores, Bacterial D013696:Temperature D058679:Virgibacillus
Bacteriocin Virgibacillus salexigens Fermented shrimp paste Antibacterial-substance