The mammalian autophagy initiator complex contains 2 HORMA domain proteins.
ATG101 is an essential component of the ULK complex responsible for initiating cellular autophagy in mammalian cells; its 3-dimensional structure and molecular function, however, are currently unclear. Here we present the X-ray structure of human ATG101. The protein displays an open HORMA domain fold. Both structural properties and biophysical evidence indicate that ATG101 is locked in this conformation, in contrast to the prototypical HORMA domain protein MAD2. Moreover, we discuss a potential mode of dimerization with ATG13 as a fundamental aspect of ATG101 function.
['Michel|Max|M|', 'Schwarten|Melanie|M|', 'Decker|Christina|C|', 'Nagel-Steger|Luitgard|L|', 'Willbold|Dieter|D|', 'Weiergräber|Oliver H|OH|']
D048868:Adaptor Proteins, Signal Transducing D000818:Animals D001343:Autophagy D018797:Cell Cycle Proteins D006801:Humans D008958:Models, Molecular D011485:Protein Binding D055503:Protein Multimerization D017434:Protein Structure, Tertiary
ATG101 ULK complex MAD2 ATG13 NMR spectroscopy X-ray crystallography autophagy-related proteins