Determination of propranolol and carvedilol in urine samples using a magnetic polyamide composite and LC-MS/MS.
AIM
β-blockers are compounds that bind with adrenoreceptors hindering their interaction with adrenalin and noradrenalin. They are clinically relevant and they are also used in some sport as doping agents.


RESULTS
A new method based on the combination of dispersive micro-solid phase extraction and LC-MS/MS has been developed to determine propranolol and carvedilol in urine samples. For this purpose a magnetic-polyamide composite is synthesized and used as sorbent. Working under the optimum conditions, the method provides limits of detection and quantification in the range of 0.1-0.15 μg/l and 0.3-0.5 μg/l, for carvedilol and propranolol, respectively. The precision, expressed as RSD, was better than 9.6% and the relative recoveries varied between 73.7 and 81.3%.


CONCLUSION
The methodology is appropriate for the determination of β-blockers in urine samples at the low microgram per liter range for therapeutic purposes.
['Ballesteros-Esteban|Tamara|T|', 'Reyes-Gallardo|Emilia M|EM|', 'Lucena|Rafael|R|', 'Cárdenas|Soledad|S|', 'Valcárcel|Miguel|M|']
D000319:Adrenergic beta-Antagonists D002227:Carbazoles D000077261:Carvedilol D002851:Chromatography, High Pressure Liquid D006801:Humans D006863:Hydrogen-Ion Concentration D057230:Limit of Detection D008280:Magnetics D008855:Microscopy, Electron, Scanning D009757:Nylons D009994:Osmolar Concentration D011412:Propanolamines D011433:Propranolol D052616:Solid Phase Extraction D053719:Tandem Mass Spectrometry D016482:Urinalysis
composite β-blockers dispersive micro-solid phase extraction