Method using in vivo quantitative spectroscopy to guide design and optimization of low-cost, compact clinical imaging devices: emulation and evaluation of multispectral imaging systems.
With recent proliferation in compact and/or low-cost clinical multispectral imaging approaches and commercially available components, questions remain whether they adequately capture the requisite spectral content of their applications. We present a method to emulate the spectral range and resolution of a variety of multispectral imagers, based on in-vivo data acquired from spatial frequency domain spectroscopy (SFDS). This approach simulates spectral responses over 400 to 1100 nm. Comparing emulated data with full SFDS spectra of in-vivo tissue affords the opportunity to evaluate whether the sparse spectral content of these imagers can (1) account for all sources of optical contrast present (completeness) and (2) robustly separate and quantify sources of optical contrast (crosstalk). We validate the approach over a range of tissue-simulating phantoms, comparing the SFDS-based emulated spectra against measurements from an independently characterized multispectral imager. Emulated results match the imager across all phantoms (<3  %   absorption, <1  %   reduced scattering). In-vivo test cases (burn wounds and photoaging) illustrate how SFDS can be used to evaluate different multispectral imagers. This approach provides an in-vivo measurement method to evaluate the performance of multispectral imagers specific to their targeted clinical applications and can assist in the design and optimization of new spectral imaging devices.
['Saager|Rolf B|RB|', 'Baldado|Melissa L|ML|', 'Rowland|Rebecca A|RA|', 'Kelly|Kristen M|KM|', 'Durkin|Anthony J|AJ|']
D000328:Adult D000818:Animals D002056:Burns D002338:Carotenoids D005260:Female D006454:Hemoglobins D006801:Humans D008297:Male D008543:Melanins D008875:Middle Aged D064847:Multimodal Imaging D061848:Optical Imaging D019047:Phantoms, Imaging D051381:Rats D012867:Skin D015595:Skin Aging D013057:Spectrum Analysis D055815:Young Adult
multispectral imaging reflectance spectroscopy tissue optics