Proteomic analysis of stromal proteins in different stages of colorectal cancer establishes Tenascin-C as a stromal biomarker for colorectal cancer metastasis.
Tumor microenvironment is crucial to tumor development and metastasis. Little is known about the roles of stromal proteins in colorectal carcinogenesis. In this study, we used a combination of laser capture microdissection (LCM), iTRAQ labeling and two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) to compare stromal proteomes in different stages of colorectal cancer. A total of 1966 proteins were identified, and 222 proteins presenting a significant fold change were quantified in different stages. Differentially expressed proteins (DEPs) were subjected to cluster and pathway analyses. We confirmed the differential expression of Tenascin-C and S100A9 using immunohistochemical analysis, and found that the expression levels of S100A9 and Tenascin-C were correlated with TNM stages and metastasis. In addition, our results showed that Tenascin-C was abundantly secreted by the colon cancer cells with high metastatic potential, and highly expressed in lymph nodes with metastasis. Our studies not only shed light on the mechanism by which stromal proteins contributed to colorectal carcinogenesis, but also identified Tenascin-C as a potential stromal biomarker for colorectal cancer metastasis.
['Li|Maoyu|M|', 'Peng|Fang|F|', 'Li|Guoqing|G|', 'Fu|Yang|Y|', 'Huang|Ying|Y|', 'Chen|Zhuchu|Z|', 'Chen|Yongheng|Y|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D014408:Biomarkers, Tumor D040502:Calgranulin B D063646:Carcinogenesis D002853:Chromatography, Liquid D015179:Colorectal Neoplasms D005260:Female D015972:Gene Expression Regulation, Neoplastic D045325:HCT116 Cells D019073:HT29 Cells D006801:Humans D007150:Immunohistochemistry D061346:Laser Capture Microdissection D008207:Lymphatic Metastasis D008297:Male D008875:Middle Aged D009367:Neoplasm Staging D040901:Proteomics D017154:Stromal Cells D053719:Tandem Mass Spectrometry D019063:Tenascin D059016:Tumor Microenvironment
Tenascin-C stromal biomarker colorectal carcinoma multistage carcinogenesis quantitative proteomics