A Fast and Reliable Method to Isolate Human Placental Macrophages.
Macrophages are specialized cells involved in recognition, uptake, and destruction of microorganisms. Human placental macrophages are poorly investigated because of the lack of a convenient protocol for their isolation. Here, we present a straightforward and reliable method to isolate macrophages from full-term human placentas. After enzymatic digestion of placental tissue and centrifugation of the cell suspension on a Ficoll cushion, placental macrophages are selected using magnetic beads coated with anti-CD14 antibodies. Isolated cells are characterized by flow cytometry. Ninety eight percent of isolated CD14+ placental macrophages also express the macrophage marker CD68. Thus, this efficient and reliable method yields placental macrophages at high purity and sufficient quantity for functional studies. © 2019 by John Wiley & Sons, Inc.
['Mezouar|Soraya|S|', 'Ben Amara|Amira|A|', 'Chartier|Céline|C|', 'Gorvel|Laurent|L|', 'Mege|Jean-Louis|JL|']
D002469:Cell Separation D005260:Female D006801:Humans D008264:Macrophages D010920:Placenta D011247:Pregnancy
placental macrophages enzymatic digestion Ficoll cushion flow cytometry CD14 selection