Repeated ethanol production from sweet sorghum juice concentrated by membrane separation.
Sequential batch fermentation from sweet sorghum juice concentrated by membrane separation (ultrafiltration permeation and nanofiltration concentration) to increase sugar contents, was investigated. Ethanol production at 5th batch fermentation by Saccharomyces cerevisiae BY4741 attained 113.7±3.1 g L(-1) (89.1±2.2% of the theoretical ethanol yield) from 270.0±22.6 g L(-1) sugars, corresponding to 98.7% of ethanol titer attained at the 1st batch fermentation. This titer was comparable to ethanol production of 115.8±0.6 g L(-1) (87.1±2.7% of the theoretical ethanol yield) obtained at 5th batch fermentation with 3 g L(-1) yeast extract and 6 g L(-1) polypeptone. Increase of cell density in the concentrated sweet sorghum juice was observed during sequential batch fermentation, as indicated by increased OD600. Utilization of sweet sorghum juice as the sole source, membrane separation, and S. cerevisiae was a cost-effective process for high ethanol production.
['Sasaki|Kengo|K|', 'Tsuge|Yota|Y|', 'Sasaki|Daisuke|D|', 'Kawaguchi|Hideo|H|', 'Sazuka|Takashi|T|', 'Ogino|Chiaki|C|', 'Kondo|Akihiko|A|']
D000431:Ethanol D005285:Fermentation D005374:Filtration D000067030:Fruit and Vegetable Juices D008567:Membranes, Artificial D012441:Saccharomyces cerevisiae D045868:Sorghum
Sweet sorghum juice Sequential batch fermentation Nanofiltration Saccharomyces cerevisiae Bioethanol