Complete genomes of the eukaryotic poultry parasite Histomonas meleagridis: linking sequence analysis with virulence / attenuation.
BACKGROUND
Histomonas meleagridis is a protozoan parasite and the causative agent of histomonosis, an important poultry disease whose significance is underlined by the absence of any treatment and prophylaxis. The recent successful in vitro attenuation of the parasite urges questions about the underlying mechanisms.


RESULTS
Whole genome sequence data from a virulent and an attenuated strain originating from the same parental lineage of H. meleagridis were recruited using Oxford Nanopore Technology (ONT) and Illumina platforms, which were combined to generate megabase-sized contigs with high base-level accuracy. Inspecting the genomes for differences identified two substantial deletions within a coding sequence of the attenuated strain. Additionally, one single nucleotide polymorphism (SNP) and indel targeting coding sequences caused the formation of premature stop codons, which resulted in the truncation of two genes in the attenuated strain. Furthermore, the genome of H. meleagridis was used for characterizing protein classes of clinical relevance for parasitic protists. The comparative analysis with the genomes of Trichomonas vaginalis, Tritrichomonas foetus and Entamoeba histolytica identified ~ 2700 lineage-specific gene losses and 9 gene family expansions in the H. meleagridis lineage.


CONCLUSIONS
Taken as a whole, the obtained data provide the first hints to understand the molecular basis of attenuation in H. meleagridis and constitute a genomics platform for future research on this important poultry pathogen.
['Palmieri|Nicola|N|', 'de Jesus Ramires|Marcelo|M|', 'Hess|Michael|M|', 'Bilic|Ivana|I|http://orcid.org/0000-0003-3296-5117']
D000818:Animals D056890:Eukaryota D010271:Parasites D011200:Poultry D017421:Sequence Analysis D014774:Virulence
Genome Histomonas meleagridis Virulence Virulence Attenuated Histomonosis Oxford Nanopore technology (ONT) Illumina Chicken Leucine rich repeat protein Turkey Virulent