Silver nanoparticles protect human keratinocytes against UVB radiation-induced DNA damage and apoptosis: potential for prevention of skin carcinogenesis.
UNLABELLED
Ultraviolet (UV)-B radiation from the sun is an established etiological cause of skin cancer, which afflicts more than a million lives each year in the United States alone. Here, we tested the chemopreventive efficacy of silver-nanoparticles (AgNPs) against UVB-irradiation-induced DNA damage and apoptosis in human immortalized keratinocytes (HaCaT). AgNPs were synthesized by reduction-chemistry and characterized for their physicochemical properties. AgNPs were well tolerated by HaCaT cells and their pretreatment protected them from UVB-irradiation-induced apoptosis along with significant reduction in cyclobutane-pyrimidine-dimer formation. Moreover, AgNPs pre-treatment led to G1-phase cell-cycle arrest in UVB-irradiated HaCaT cells. AgNPs were efficiently internalized in UVB-irradiated cells and localized into cytoplasmic and nuclear compartments. Furthermore, we observed an altered expression of various genes involved in cell-cycle, apoptosis and nucleotide-excision repair in HaCaT cells treated with AgNPs prior to UVB-irradiation. Together, these findings provide support for potential utility of AgNPs as novel chemopreventive agents against UVB-irradiation-induced skin carcinogenesis.


FROM THE CLINICAL EDITOR
Excessive exposure to the sun is known to increase the risk of skin cancer due to DNA damage. In this work, the authors tested the use of silver nanoparticles as protective agents against ultraviolet radiation. The positive results may open a door for the use of silver nanoparticle as novel agents in the future.
['Arora|Sumit|S|', 'Tyagi|Nikhil|N|', 'Bhardwaj|Arun|A|', 'Rusu|Lilia|L|', 'Palanki|Rohan|R|', 'Vig|Komal|K|', 'Singh|Shree R|SR|', 'Singh|Ajay P|AP|', 'Palanki|Srinivas|S|', 'Miller|Michael E|ME|', 'Carter|James E|JE|', 'Singh|Seema|S|']
D016588:Anticarcinogenic Agents D017209:Apoptosis D002460:Cell Line D004249:DNA Damage D004260:DNA Repair D006801:Humans D015603:Keratinocytes D053768:Metal Nanoparticles D012834:Silver D012878:Skin Neoplasms D014466:Ultraviolet Rays
Silver nanoparticles Keratinocytes DNA damage Apoptosis Ultraviolet radiations Cyclobutane pyrimidine dimers (CPDs) Nucleotide excision repair (NER)