Carotid Endarterectomy Induces the Release of Inflammatory Markers and the Activation of Coagulation as Measured in the Jugular Bulb.
BACKGROUND AND PURPOSE
Transient cerebral hypoxia may induce neuronal injury through an ischemia-reperfusion (I/R) response, with a subsequent activation of inflammation and coagulation-fibrinolysis. During carotid endarterectomy (CEA), the artery is clamped, which might impair the regional cerebral perfusion and initiate a local I/R response. Data suggest that the CD40-CD40 ligand dyad acts as a modulator in the induced activation. The aim of this study was to locally measure soluble CD40 ligand (sCD40L), in conjunction with inflammation and coagulation activation markers, during CEA.


SUBJECTS AND METHODS
This is a prospective study of 18 patients undergoing CEA. Blood samples from the venous jugular bulb (JB) and the radial artery (RA) were drawn at baseline and during the procedure. Measurements of sCD40L, interleukin-6 (IL-6), fragment 1 + 2 (F1 + 2), plasminogen activator inhibitor-1 (PAI-1), and d-dimer were analyzed. Comparisons during CEA were made between levels: baselines versus JB, JB versus RA, and sequential JB measurements. Fifty cardiovascular healthy patients were the reference group for the sCD40L baseline comparison.


RESULTS
Increased cerebral IL-6 levels were demonstrated throughout the procedure, as well as the temporal influence in F1 + 2, PAI-1, and d-dimer values. sCD40L remained unchanged throughout the procedure . This indicates a local cerebral inflammatory reaction together with an activation of coagulation-fibrinolysis, but it does not appear to primarily involve the CD40-CD40 ligand dyad.


CONCLUSIONS
Signs of a local inflammatory reaction and activation of coagulation were observed during CEA, but levels of sCD40L remained stable, unaffected by carotid artery clamping and reperfusion.
['Kragsterman|Bjorn|B|', 'Bergqvist|David|D|', 'Siegbahn|Agneta|A|', 'Parsson|Hakan|H|']
D000368:Aged D015415:Biomarkers D023201:CD40 Ligand D002343:Carotid Artery, Internal D016893:Carotid Stenosis D016894:Endarterectomy, Carotid D005260:Female D005338:Fibrin Fibrinogen Degradation Products D006801:Humans D015850:Interleukin-6 D007601:Jugular Veins D008297:Male D008875:Middle Aged D017395:Plasminogen Activator Inhibitor 1 D011446:Prospective Studies
Carotid endarterectomy cerebral perfusion carotid clamping cerebral ischemia