Pro-apoptotic effect of new quinolone 7- ethyl 9-ethyl-6-oxo-6,9-dihydro[1,2,5]selenadiazolo [3,4-h]quinoline-7-carboxylate on cervical cancer cell line HeLa alone/with UVA irradiation.
7- ethyl 9-ethyl-6-oxo-6,9-dihydro[1,2,5]selenadiazolo [3,4-h]quinoline-7-carboxylate (E2h) is a new synthetically prepared quinolone derivative, which in our primary study showed cytotoxic effects towards tumor cells. The aim of the present study was to examine the antiproliferative and apoptosis inducing activities of E2h towards human cervical cancer cell line HeLa with/without the presence of UVA irradiation. Further, the molecular mechanism involved in E2h-induced apoptosis in HeLa cells was investigated. Our results showed that both non-photoactivated and photoactivated E2h caused morphological changes and inhibited the cell growth of HeLa cells in a time- and dose-dependent manner. Irradiation increased the sensitivity of HeLa cells to E2h. Quinolone induced S and G2/M arrest and apoptosis in HeLa cells, as characterized by DNA fragmentation and flow cytometry. In addition, E2h elevated the level of reactive oxygen species and activated caspases 3. In conclusions, E2h alone/in combination with UVA irradiation induced apoptosis in HeLa cells through the ROS-mitochondrial/caspase 3-dependent pathway.
['Jantová|Soňa|S|', 'Mrvová|Nataša|N|', 'Hudec|Roman|R|', 'Sedlák|Ján|J|', 'Pánik|Miroslav|M|', 'Milata|Viktor|V|']
D000970:Antineoplastic Agents D017209:Apoptosis D002470:Cell Survival D005260:Female D006367:HeLa Cells D006801:Humans D053078:Membrane Potential, Mitochondrial D016566:Organoselenium Compounds D015363:Quinolones D017382:Reactive Oxygen Species D014466:Ultraviolet Rays D002583:Uterine Cervical Neoplasms
UVA irradiation Quinolone derivative Apoptosis HeLa cells Cell cycle arrest