Insights into epidemiology of human parvovirus B19 and detection of an unusual genotype 2 variant, Bulgaria, 2004 to 2013.
The present study aimed to determine the role of human parvovirus В19 (B19V) as an aetiological agent in measles and rubella negative fever/rash patients from Bulgaria between 2004 and 2013. A total of 1,266 sera from all over the country were tested for B19V IgM antibodies and all positives were further investigated by polymerase chain reaction (PCR). Overall, 280 sera (22%) were B19V IgM positive and 227 of these (81%) were also PCR positive. The highest number of IgM positives was found among five to nine year-old children (27%). Eight infected women gave birth to healthy children; one fetus was aborted with hydrops fetalis. Of the 55 genetic sequences obtained, 54 belonged to genotype 1a and one grouped as a genotype 2 outlier. Phylogenetic analysis of all available genotype 2 sequences covering the 994 nucleotide non-structural protein 1(NS1)/capsid viral protein 1 (VP1) unique region junction, showed that only one other sequence grouped with the outlier strain, forming a clearly distinct and well-supported cluster of genotype 2 (between-group genetic distance: 3.32%). In accordance with B19V nomenclature, this cluster may represent a new subgenotype 2b. The study showed that B19V infections may be falsely identified as rubella or measles in ca 22% of cases, emphasising the need for laboratory confirmation.
['Ivanova|Stefka Krumova|SK|', 'Mihneva|Zafira Georgieva|ZG|', 'Toshev|Andon Krumov|AK|', 'Kovaleva|Valentina Pavlova|VP|', 'Andonova|Lubena Georgieva|LG|', 'Muller|Claude P|CP|', 'Hübschen|Judith M|JM|']
D000293:Adolescent D000328:Adult D017677:Age Distribution D000914:Antibodies, Viral D002031:Bulgaria D002648:Child D002675:Child, Preschool D016731:Erythema Infectiosum D005076:Exanthema D005260:Female D005334:Fever D014644:Genetic Variation D005838:Genotype D006801:Humans D007075:Immunoglobulin M D007223:Infant D008297:Male D008875:Middle Aged D017720:Molecular Epidemiology D008969:Molecular Sequence Data D016732:Parvovirus B19, Human D010802:Phylogeny D016133:Polymerase Chain Reaction D014764:Viral Proteins D055815:Young Adult
human parvovirus B19 genotype IgM antibodies PCR ELISA