An ABC transporter involved in the control of streptomycin production in Streptomyces griseus.
We screened for a gene that inhibits streptomycin production in Streptomyces griseus when it is introduced on a high-copy-number plasmid pIJ702, and obtained a plasmid pKM545. The introduction of pKM545 abolished streptomycin production on all media tested including YMP-sugar and Nutrient broth. S1 protection analysis demonstrated that the introduction of this plasmid downregulated the transcriptional activity of the promoter preceding strR, the pathway-specific transcriptional regulator for streptomycin biosynthesis. The 2.8-kb BamHI fragment cloned onto pKM545 contained two coding sequences SGR_5442 and 5443. These coding sequences and the two downstream ones (SGR_5444 and 5445) constituted a possible operon structure designated to be rspABCD (regulation of streptomycin production). RspB and RspC exhibited a marked similarity with an ATP-binding domain and a membrane-associating domain of an ABC-2 type transporter, respectively, suggesting that the Rsp proteins comprise a membrane exporter. The gene cluster consisting of the rsp operon and the upstream divergent small coding sequence (SGR_5441) was widely distributed to Streptomyces genome. An rspB mutant of S. griseus produced 3-fold streptomycin of the parental strain in YMP liquid medium. The evidence implies that the Rsp translocator is involved in the export of a substance that specifies the expression level of streptomycin biosynthesis genes in S. griseus.
['Takano|Hideaki|H|', 'Toriumi|Naoe|N|', 'Hirata|Mariko|M|', 'Amano|Taisuke|T|', 'Ohya|Takaaki|T|', 'Shimada|Reona|R|', 'Kusada|Hiroyuki|H|', 'Amano|Sho-Ichi|S|', 'Matsuda|Ko-Ichi|K|', 'Beppu|Teruhiko|T|', 'Ueda|Kenji|K|']
D018528:ATP-Binding Cassette Transporters D000595:Amino Acid Sequence D001426:Bacterial Proteins D003001:Cloning, Molecular D023061:Gene Order D056426:Genetic Loci D009154:Mutation D010957:Plasmids D054730:Protein Interaction Domains and Motifs D013305:Streptomyces griseus D013307:Streptomycin
ABC transporter streptomycin Streptomyces griseus