Facilitating enzymatic hydrolysis with a novel guaiacol-based deep eutectic solvent pretreatment.
Herein, we established a novel deep eutectic solvent (DES) using lignin-derived guaiacol as hydrogen bond donor (HBD). The sole ChCl/guaiacol system was found to be inefficient for the fractionation of wheat straw (WS), while the incorporation of trace AlCl3 significantly facilitated the degradation of hemicellulose and lignin, resulting in a complete enzymatic digestibility of the pretreated WS. Further, this study revealed that the DES-degraded lignin was readily precipitated during the washing process, and thus hindered the enzymatic hydrolysis of poplar and bamboo (with hydrolysis yield of 42.03% and 71.67%, respectively). Alkali washing offers a possible approach to remove the precipitated lignin, after which a near 100% hydrolysis yield was also obtained for poplar and bamboo.
['Huang|Chen|C|', 'Zhan|Yunni|Y|', 'Cheng|Jinyuan|J|', 'Wang|Jia|J|', 'Meng|Xianzhi|X|', 'Zhou|Xuelian|X|', 'Fang|Guigan|G|', 'Ragauskas|Arthur J|AJ|']
D018533:Biomass D006139:Guaiacol D006868:Hydrolysis D008031:Lignin D012997:Solvents
Enzymatic hydrolysis Deep eutectic solvent Guaiacol