Bacterial diversity in ballast water and sediments revealed by 2b-RAD sequencing.
Despite that the ballast water management (BWM) convention has come into force to prevent the spread of harmful aquatic organisms, to date, very few bacteria can be identified through microbial culture method. In this study, we explored a reduced-representation sequencing of 2b-RAD approach to investigate the bacterial diversity in ballast water and sediments (BWS). Our results indicated a large amount of bacteria species (1496) detected in BWS up to now, including 13 pathogens that are seriously concerning in marine environment and aquaculture like the most harmful Vibrio harveyi and Aurantimonas coralicida. We showed that the ballast water had relative lower species, which was dominated by Proteobacteria. In contrast, the sediments had richer species, which was dominated by Bacteroidetes. Although BWS differed significantly in species composition, sediments shared most of the concerned pathogens with ballast water, highlighting the importance of sediment management. In conclusion, 2b-RAD sequencing shows promise in future BWM.
['Xue|Zhaozhao|Z|', 'Han|Yangchun|Y|', 'Liu|Bingli|B|', 'Gu|Yujuan|Y|', 'Tian|Wen|W|', 'Whiting-Wagner|Nathan|N|', 'Zhao|Hong|H|', 'Zhang|Wei|W|']
D020561:Alphaproteobacteria D012767:Ships D014733:Vibrio D014867:Water
2b-RAD Ballast water management Reduced-representation sequencing Bacteria monitoring Marine pathogen