Using Bioengineered Fluorescence for Selective In Vivo and Ex Vivo Tracking of Intestinal Organoids Derived from Human Pluripotent Stem Cells.
For current and future applications of human intestinal organoids (hIOs) to various aspects of in vivo research and their potential clinical use, an efficient noninvasive system is needed to directly visualize the stage of intestinal differentiation and graft-host interactions and for further safety monitoring and efficacy. Here, we describe a detailed method for monitoring and histologically identifying implanted hIO-expressing eGFP and mCherry fluorescence under the kidney capsule of immunodeficient mice with fluorescence imaging (FLI). We then describe the orthotropic transplantation method of hIOs and methods to confirm successful engraftment in the small intestines of immunodeficient mice. These methods provide an approach for tracking the location of intestinal cells in hIOs in vivo and ex vivo using a fluorescent reporter system from the beginning of engraftment to various subsequent experiments.
['Lee|Hana|H|', 'Son|Mi-Young|MY|']
D000818:Animals D002454:Cell Differentiation D058948:Cell Tracking D003092:Colitis D005260:Female D005453:Fluorescence D006801:Humans D007413:Intestinal Mucosa D007421:Intestine, Small D008297:Male D051379:Mice D016688:Mice, Inbred NOD D016513:Mice, SCID D009940:Organoids D039904:Pluripotent Stem Cells
Human intestinal organoid Fluorescence imaging Reporter system Human induced pluripotent stem cell In vivo transplantation Intraintestinal injection