Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine.
Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and amoxicillin were the most effective drugs with susceptibility higher than 97%; however, 76.79% of poultry strains and 85% of swine strains were resistant to sulphonamides. Furthermore, 19.64% and 36.58% of avian and swine strains, respectively, were multi-resistant. Virulence genes studied were not specific to a host and may be the result of horizontal transmission throughout evolution. High multidrug resistance demonstrates the need for responsible use of antimicrobials in animals intended for human consumption, in addition to antimicrobial susceptibility testing to P. multocida.
['Furian|Thales Quedi|TQ|', 'Borges|Karen Apellanis|KA|', 'Laviniki|Vanessa|V|', 'Rocha|Silvio Luis da Silveira|SL|', 'de Almeida|Camila Neves|CN|', 'do Nascimento|Vladimir Pinheiro|VP|', 'Salle|Carlos Tadeu Pippi|CT|', 'Moraes|Hamilton Luiz de Souza|HL|']
D000818:Animals D004269:DNA, Bacterial D024881:Drug Resistance, Bacterial D005838:Genotype D008826:Microbial Sensitivity Tests D010326:Pasteurella Infections D016979:Pasteurella multocida D016133:Polymerase Chain Reaction D011200:Poultry D011201:Poultry Diseases D012703:Serotyping D013552:Swine D013553:Swine Diseases D037521:Virulence Factors
Virulence factors Antimicrobial susceptibility Multiplex-PCR Pasteurellosis