Marinobacter denitrificans sp. nov., isolated from marine sediment of southern Scott Coast, Antarctica.
A novel bacterium, designated JB02H27T, was isolated from marine sediment collected from the southern Scott Coast, Antarctica. Cells were Gram-stain-negative, facultatively anaerobic, polar-ﬂagellated and motile rods. Growth occurred at 4-45 °C, at pH 7.0-9.0 and with 3-25 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain JB02H27T consistently fell within the genus Marinobacter and formed a clade together with Marinobacter algicola DG893T (98.8 % similarity), Marinobacter confluentis KCTC 42705T (98.4 %), Marinobacter salarius R9SW1T (98.4%) and Marinobacter halotolerans CP12T (97.9 %), which were subsequently used as reference strains for comparisons of phenotypic and chemotaxonomic characteristics. Average nucleotide identity values between strain JB02H27T and the four related type strains were 80.9, 76.6, 81.9 and 76.3 %, respectively. The major fatty acids were summed feature 3, C16 : 0, C18 : 1 ω9c and C16 : 0 N alcohol. The polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unidentified phospholipid, aminolipid, aminophospholipid and glycolipids. The sole respiratory quinone was ubiquinone-9. The DNA G+C content was 56.9 mol%. Based on the genomic, phylogenetic, phenotypic and chemotaxonomic analysis, we propose that strain JB02H27T represents a novel species of the genus Marinobacter, for which the name Marinobacter denitrificans sp. nov. is proposed. The type strain is JB02H27T (=GDMCC 1.1528T=KCTC 62941T).
['Zhang|Ming-Xia|MX|', 'Li|An-Zhang|AZ|', 'Wu|Qingping|Q|', 'Yao|Qing|Q|', 'Zhu|Hong-Hui|HH|']
D000864:Antarctic Regions D015373:Bacterial Typing Techniques D001482:Base Composition D004269:DNA, Bacterial D005227:Fatty Acids D019015:Geologic Sediments D006017:Glycolipids D053520:Marinobacter D010743:Phospholipids D010802:Phylogeny D012336:RNA, Ribosomal, 16S D012623:Seawater D017422:Sequence Analysis, DNA D014451:Ubiquinone
Marinobacter marine sediment southern Scott Coast JB02H27