The Defensive Role of Cumulus Cells Against Reactive Oxygen Species Insult in Metaphase II Mouse Oocytes.
We investigated the ability of reactive oxygen species (ROS), such as hydrogen peroxide (H(2)O(2)), hydroxyl radical ((·)OH), and hypochlorous acid (HOCl), to overcome the defensive capacity of cumulus cells and elucidate the mechanism through which ROS differentially deteriorate oocyte quality. Metaphase II mouse oocytes with (n = 1634) and without cumulus cells (n = 1633) were treated with increasing concentration of ROS, and the deterioration in oocyte quality was assessed by the changes in the microtubule morphology and chromosomal alignment. Oocyte and cumulus cell viability and cumulus cell number were assessed by indirect immunofluorescence, staining of gap junction protein, and trypan blue staining. The treated oocytes showed decreased quality as a function of increasing concentrations of ROS when compared to controls. Cumulus cells show protection against H(2)O(2) and (·)OH insult at lower concentrations, but this protection was lost at higher concentrations (>50 μmol/L). At higher H(2)O(2) concentrations, treatment dramatically influenced the cumulus cell number and viability with resulting reduction in the antioxidant capacity making the oocyte more susceptible to oxidative damage. However, cumulus cells offered no significant protection against HOCl at any concentration used. In all circumstances in which cumulus cells did not offer protection to the oocyte, both cumulus cell number and viability were decreased. Therefore, the deterioration in oocyte quality may be caused by one or more of the following: a decrease in the antioxidant machinery by the loss of cumulus cells, the lack of scavengers for specific ROS, and/or the ability of the ROS to overcome these defenses.
['Shaeib|Faten|F|', 'Khan|Sana N|SN|', 'Ali|Iyad|I|', 'Thakur|Mili|M|', 'Saed|Mohammed G|MG|', 'Dai|Jing|J|', 'Awonuga|Awoniyi O|AO|', 'Banerjee|Jashoman|J|', 'Abu-Soud|Husam M|HM|']
D000818:Animals D002470:Cell Survival D054885:Cumulus Cells D004305:Dose-Response Relationship, Drug D005260:Female D006861:Hydrogen Peroxide D008677:Metaphase D051379:Mice D008810:Mice, Inbred C57BL D009865:Oocytes D017382:Reactive Oxygen Species
cumulus cells reactive oxygen species hydrogen peroxide oocyte quality and inflammation oxidative stress