Nitric oxide and lipid peroxidation in children, residents of the contaminated territories.
OBJECTIVE
The nitrogen oxide system assessment by the nitrogen oxide stable metabolites assay both with indices of lipid peroxidation and antioxidant protection of children residing on contaminated territories.


MATERIALS AND METHODS
Studies were conducted in children, born and residing in areas of contamination. Assay of nitrogenous species (NO²⁻ and NO³⁻) was performed using a standard method with Griss-reagent. Biochemical assay of the malonic dyaldehide, catalase, and ceruloplasmin was applied to reveal the signs of oxidative stress.


RESULTS AND CONCLUSIONS
The assay of nitric oxide metabolites in the blood serum of children-residents of contaminated territories revealed a reduction of nitrite and no significant changes in the content of both nitrate and total NO metabolites. There was an activation of the intracellular antioxidant catalase with the absence of significant changes in the basic serum ceruloplasmin antioxidant. All that was not accompanied with an increase of serum concentration of malonic dyaldehide i.e. the TBA-active product of lipid peroxidation being indicative to the adequacy of compensatory capacity of antioxidant defense.
['Stepanova|Y|Y|', 'Kolpakov|I|I|', 'Kondrashova|V|V|', 'Litvinets|O|O|']
D000293:Adolescent D000975:Antioxidants D047852:Chernobyl Nuclear Accident D002648:Child D004781:Environmental Exposure D006801:Humans D015227:Lipid Peroxidation D008054:Lipid Peroxides D009569:Nitric Oxide D011830:Radiation Effects D014455:Ukraine
nitric oxide lipid peroxidation children contaminated territories Chornobyl accident