Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate.
BACKGROUND/AIMS
A reproducible, endoscope-based, large animal model, of acute pancreatitis was developed to meet the need for a suitable means of preclinically testing treatments. The aim of this study was to develop an endoscope-based animal model of acute pancreatitis.


METHODS
This experimental study was conducted on six mini-pigs. The pancreatitis model was induced by infusing contrast medium (CM) or sodium taurocholate (TCA) under high pressure (100 mmHg) into the main pancreatic duct by endoscopic retrograde pancreatography. Animals were randomly allocated to three groups: a CM group, a 10% TCA group, and a 20% TCA group. Pancreatic injuries were evaluated histologically, and serum amylase and lipase levels were measured.


RESULTS
Acute pancreatitis was observed in all animals during hematologic and histologic examinations. Serum amylase and lipase levels were significantly higher (> 10 times baseline), and pancreatic edema, vacuolization of acinar cells, and hemorrhagic necrosis were observed. Severity of pancreatitis tended to be greater in the TCA groups than in the CM group as assessed using histologic scores, and degrees of pancreatitis were found to be dose-dependently related to TCA concentration.


CONCLUSION
The two endoscopic procedures described are effective and safe for creating a swine model of acute pancreatitis. The authors hope the described endoscopic methods will assist in the development of a suitable treatment strategy.
['Park|Jin-Seok|JS|', 'Jeong|Seok|S|', 'Kim|Joon Mee|JM|', 'Lee|Bum Hei|BH|', 'Kim|Jae Min|JM|', 'Lee|Don Haeng|DH|']
D000681:Amylases D000818:Animals D015415:Biomarkers D002760:Cholangiopancreatography, Endoscopic Retrograde D003287:Contrast Media D004195:Disease Models, Animal D007263:Infusions, Parenteral D008049:Lipase D010179:Pancreas D010195:Pancreatitis D013552:Swine D013556:Swine, Miniature D013656:Taurocholic Acid D013997:Time Factors
Pancreatitis Endoscopes Animals