Phenotypic and Functional Characterization of Double Negative B Cells in the Blood of Individuals With Obesity.
We have previously shown that obesity is associated with increased secretion of IgG antibodies with anti-self-reactivity. In this paper, we confirm and extend our previous findings. We show that the plasma of individuals with obesity is enriched in autoimmune antibodies whose levels are positively associated with blood frequencies of the subset of Double Negative (DN) B cells, which is the most pro-inflammatory B cell subset. We also show that DN B cells, significantly increased in the blood of obese versus lean individuals, are characterized by higher expression of immune activation markers and of the transcription factor T-bet, both associated with autoimmunity. The removal of DN B cells from the peripheral B cell pool significantly decreases in vitro secretion of anti-self IgG antibodies. These results altogether confirm the crucial role of DN B cells in the secretion of anti-self IgG antibodies in individuals with obesity.
['Frasca|Daniela|D|', 'Diaz|Alain|A|', 'Romero|Maria|M|', 'Blomberg|Bonnie B|BB|']
D000328:Adult D001323:Autoantibodies D001324:Autoantigens D015551:Autoimmunity D016175:B-Lymphocyte Subsets D001402:B-Lymphocytes D015415:Biomarkers D004198:Disease Susceptibility D005260:Female D006801:Humans D007074:Immunoglobulin G D016130:Immunophenotyping D018655:Lymphocyte Count D008212:Lymphocyte Depletion D008297:Male D008875:Middle Aged D009765:Obesity
B cells obesity aging antibody responses inflammation