Insulin-like growth factor-1 regulation of retinal progenitor cell proliferation and differentiation.
Strategies to improve retinal progenitor cell (RPC) capacity to yield proliferative and multipotent pools of cells that can efficiently differentiate into retinal neurons, including photoreceptors, could be vital for cell therapy in retinal degenerative diseases. In this study, we found that insulin-like growth factor-1 (IGF-1) plays a role in the regulation of proliferation and differentiation of RPCs. Our results show that IGF-1 promotes RPC proliferation via IGF-1 receptors (IGF-1Rs), stimulating increased phosphorylation in the PI3K/Akt and MAPK/Erk pathways. An inhibitor experiment revealed that IGF-1-induced RPC proliferation was inhibited when the PI3K/Akt and MAPK/Erk pathways were blocked. Furthermore, under the condition of differentiation, IGF-1-pretreated RPCs prefer to differentiate into retinal neurons, including photoreceptors, in vitro, which is crucial for visual formation and visual restoration. These results demonstrate that IGF-1 accelerates the proliferation of RPCs and IGF-1 pretreated RPCs may have shown an increased potential for retinal neuron differentiation, providing a novel strategy for regulating the proliferation and differentiation of retinal progenitors in vitro and shedding light upon the application of RPCs in retinal cell therapy.
['Wang|Yuyao|Y|', 'Zhang|Dandan|D|', 'Zhang|Yi|Y|', 'Ni|Ni|N|', 'Tang|Zhimin|Z|', 'Bai|Zhisha|Z|', 'Shen|Bingqiao|B|', 'Sun|Hao|H|', 'Gu|Ping|P|']
D000818:Animals D002454:Cell Differentiation D049109:Cell Proliferation D002478:Cells, Cultured D004815:Epidermal Growth Factor D007334:Insulin-Like Growth Factor I D020935:MAP Kinase Signaling System D051379:Mice D008810:Mice, Inbred C57BL D019869:Phosphatidylinositol 3-Kinases D000081082:Phosphoinositide-3 Kinase Inhibitors D047428:Protein Kinase Inhibitors D051057:Proto-Oncogene Proteins c-akt D012160:Retina D055351:Retinal Neurons D015398:Signal Transduction D013234:Stem Cells
proliferation differentiation Retinal progenitor cell (RPC) insulin-like growth factor-1 (IGF-1) signal pathway