Role of the Unconventional Prefoldin Proteins URI and UXT in Transcription Regulation.
The Unconventional prefoldin RPB5 interacting protein (URI), also known as RPB5-Mediating Protein (RMP) has been shown to play several regulatory roles in different cellular compartments including the mitochondria, as a phosphatase binding protein; in the cytoplasm, as a chaperone-like protein; and in the nucleus, as a transcriptional regulator through binding to RPB5 and RNA polymerase II (polII). This chapter focuses on the role URI plays in transcriptional regulation in the prostate cell. In prostate cells, URI is tightly bound to another prefoldin-like protein called UXT, a known androgen receptor (AR) cofactor. Part of a multiprotein complex, URI and UXT act as transcriptional repressors, and URI regulates KAP1 through PP2A phosphatase activity. The discovery of the interaction of URI and UXT with KAP1, AR, and PP2A, as well as the numerous interactions between URI and components of the R2TP/prefoldin-like complex, RPB5, and nuclear proteins involved in DNA damage response, chromatin remodeling and gene transcription, reveal a pleiotropic effect of the URI/UXT complex on nuclear processes. The mechanisms by which URI/UXT affect transcription, chromatin structure and regulation, and genome stability, remain to be elucidated but will be of fundamental importance considering the many processes affected by alterations of URI/UXT and other prefoldins and prefoldin-like proteins.
['Thomas|Phillip A|PA|', 'Mita|Paolo|P|', 'Ha|Susan|S|', 'Logan|Susan K|SK|']
D018797:Cell Cycle Proteins D012321:DNA-Directed RNA Polymerases D005786:Gene Expression Regulation D006801:Humans D047908:Intracellular Signaling Peptides and Proteins D008297:Male D018832:Molecular Chaperones D009363:Neoplasm Proteins D011467:Prostate D012097:Repressor Proteins
Unconventional prefoldin RPB5 interacting protein (URI) Androgen Receptor (AR) Androgen Receptor Trapped clone-27 (ART-27) Gene transcription, prostate cancer KRAB-associated protein 1 (KAP1) Long Interspersed Element-1 (LINE-1) Protein phosphatase PP2A Proteomics Retroelements Ubiquitously Expressed Transcript (UXT)