Antibacterial and antioxidative activity of O-amine functionalized chitosan.
Cinnamaldehyde was immobilized to O-amine functionalized chitosan via a coupling reaction. Fourier transform infrared spectroscopy confirmed N-cinnamyl substitution. Wetting analyses demonstrate more hydrophobicity in the N-cinnamyl substituted O-amine functionalized chitosan compared to chitosan or unsubstituted O-amine functionalized chitosan. Thermal gravimetric analysis and differential scanning calorimetry demonstrates that the prepared N-cinnamyl substituted O-amine functionalized chitosan exhibits higher thermostability than unmodified chitosan at temperatures in which polysaccharides are commonly stored and utilised. The N-cinnamyl substituted O-amine functionalized chitosan, against four different bacteria strains [two gram-positive (Staphylococcus aureus and Bacillus cereus) and two gram-negative (Escherichia coli and Pseudomonas aeruginosa)], displays promotion of inhibition activity against these bacterial strains. Finally, the antioxidative activity of the N-cinnamyl substituted O-amine functionalized chitosan was compared with those activities of chitosan and O-amine functionalized chitosan. This was evaluated by uninhibited and inhibited hyaluronan degradation and ABTS assay. The N-cinnamyl substituted O-amine functionalized chitosan shows a lower activity towards donating a hydrogen radical compared to chitosan or O-amine functionalized chitosan. On the other hand, the N-cinnamyl substituted O-amine functionalized chitosan exhibited a higher ability to scavenge the ABTS+ cation radical compared to chitosan and O-amine functionalized chitosan.
['Tamer|Tamer M|TM|', 'Hassan|Mohamed A|MA|', 'Omer|Ahmed M|AM|', 'Valachová|Katarína|K|', 'Eldin|Mohamed S Mohy|MSM|', 'Collins|Maurice N|MN|', 'Šoltés|Ladislav|L|']
D000171:Acrolein D000588:Amines D000900:Anti-Bacterial Agents D000975:Antioxidants D001409:Bacillus cereus D048271:Chitosan D004926:Escherichia coli D008826:Microbial Sensitivity Tests D011550:Pseudomonas aeruginosa D013211:Staphylococcus aureus
Staphylococcus aureus Bacillus cereus Escherichia coli Pseudomonas aeruginosa Chitosan derivatives