Inhibitory and multisynaptic spines, and hemispherical synaptic specialization in the posterodorsal medial amygdala of male and female rats.
The density of dendritic spines is sexually dimorphic and variable throughout the female estrous cycle in the rat posterodorsal medial amygdala (MePD), a relevant area for the modulation of reproductive behavior in rats. The local synaptic activity differs between hemispheres in prepubertal animals. Here we used serial section transmission electron microscopy to produce 3D reconstructions of dendritic shafts and spines to characterize synaptic contacts on MePD neurons of both hemispheres in adult males and in females along the estrous cycle. Pleomorphic spines and nonsynaptic filopodia occur in the MePD. On average, 8.6% of dendritic spines received inputs from symmetric gamma-aminobutyric acid (GABA)-immunoreactive terminals, whereas 3.6% received two synaptic contacts on the spine head, neck, or base. Presynaptic terminals in female right MePD had a higher density of synaptic vesicles and docked vesicles than the left MePD, suggesting a higher rate of synaptic vesicle release in the right MePD of female rats. In contrast, males did not show laterality in any of those parameters. The proportion of putative inhibitory synapses on dendritic shafts in the right MePD of females in proestrus was higher than in the left MePD, and higher than in the right MePD in males, or in females in diestrus or estrus. This work shows synaptic laterality depending on sex and estrous cycle phase in mature MePD neurons. Most likely, sexual hormone effects are lateralized in this brain region, leading to higher synaptic activity in the right than in the left hemisphere of females, mediating timely neuroendocrine and social/reproductive behavior.
['Brusco|Janaina|J|', 'Merlo|Suélen|S|', 'Ikeda|Érika T|ÉT|', 'Petralia|Ronald S|RS|', 'Kachar|Bechara|B|', 'Rasia-Filho|Alberto A|AA|', 'Moreira|Jorge E|JE|']
D000679:Amygdala D000818:Animals D001369:Axons D003712:Dendrites D049229:Dendritic Spines D030762:Estrous Cycle D005260:Female D007839:Functional Laterality D017629:Gap Junctions D021621:Imaging, Three-Dimensional D008297:Male D046529:Microscopy, Electron, Transmission D009433:Neural Inhibition D009474:Neurons D017729:Presynaptic Terminals D017208:Rats, Wistar D012727:Sex Characteristics D013569:Synapses D013572:Synaptic Vesicles D005680:gamma-Aminobutyric Acid
electron microscopy 3D reconstruction laterality extended amygdala inhibitory dendritic spines multisynaptic dendritic spines