yVDAC2, the second mitochondrial porin isoform of Saccharomyces cerevisiae.
The yeast Saccharomyces cerevisiae genome is endowed with two distinct isoforms of Voltage-Dependent Anion Channel (VDAC). The isoform yVDAC2 is currently understudied with respect to the best known yVDAC1. Yet, since the discovery, the function of yVDAC2 was unclear, leading to the hypothesis that it might be devoid of a channel function. In this work we have elucidated, by bioinformatics modeling and electrophysiological analysis, the functional activity of yVDAC2. The conformation of yVDAC2 and, for comparison, of yVDAC1 were modeled using a multiple template approach involving mouse, human and zebrafish structures and both showed to arrange the sequences as the typical 19-stranded VDAC β-barrel. Molecular dynamics simulations showed that yVDAC2, in comparison with yVDAC1, has a different number of permeation paths of potassium and chloride ions. yVDAC2 protein was over-expressed in the S. cerevisiae cells depleted of functional yVDAC1 (Δpor1 mutant) and, after purification, it was reconstituted in artificial membranes (planar lipid bilayer (PLB) system). The protein displayed channel-forming activity and the calculated conductance, voltage-dependence and ion selectivity values were similar to those of yVDAC1 and other members of VDAC family. This is the first time that yVDAC2 channel features are detected and characterized.
['Guardiani|Carlo|C|', 'Magrì|Andrea|A|', 'Karachitos|Andonis|A|', 'Di Rosa|Maria Carmela|MC|', 'Reina|Simona|S|', 'Bodrenko|Igor|I|', 'Messina|Angela|A|', 'Kmita|Hanna|H|', 'Ceccarelli|Matteo|M|', 'De Pinto|Vito|V|']
D000818:Animals D001665:Binding Sites D002712:Chlorides D019295:Computational Biology D015870:Gene Expression D006801:Humans D017136:Ion Transport D007700:Kinetics D008051:Lipid Bilayers D051379:Mice D008928:Mitochondria D051336:Mitochondrial Membranes D056004:Molecular Dynamics Simulation D011188:Potassium D011485:Protein Binding D000072757:Protein Conformation, beta-Strand D054730:Protein Interaction Domains and Motifs D011994:Recombinant Proteins D012441:Saccharomyces cerevisiae D029701:Saccharomyces cerevisiae Proteins D040681:Structural Homology, Protein D050995:Voltage-Dependent Anion Channel 1 D050996:Voltage-Dependent Anion Channel 2 D015027:Zebrafish
Molecular dynamics Outer mitochondrial membrane (OMM) Yeast VDAC isoforms