Effect of acetochlor on transcription of genes associated with oxidative stress, apoptosis, immunotoxicity and endocrine disruption in the early life stage of zebrafish.
The study presented here aimed to characterize the effects of acetochlor on expression of genes related to endocrine disruption, oxidative stress, apoptosis and immune system in zebrafish during its embryo development. Different trends in gene expression were observed after exposure to 50, 100, 200μg/L acetochlor for 96h. Results demonstrated that the transcription patterns of many key genes involved in the hypothalamic-pituitary-gonadal/thyroid (HPG/HPT) axis (e.g., VTG1, ERβ1, CYP19a and TRα), cell apoptosis pathway (e.g., Bcl2, Bax, P53 and Cas8), as well as innate immunity (e.g., CXCL-C1C, IL-1β and TNFα) were affected in newly hatched zebrafish after exposure to acetochlor. In addition, the up-regulation of CAT, GPX, GPX1a, Cu/Zn-SOD and Ogg1 suggested acetochlor might trigger oxidative stress in zebrafish. These finding indicated that acetochlor could simultaneously induce multiple responses during zebrafish embryonic development, and bidirectional interactions among oxidative stress, apoptosis pathway, immune and endocrine systems might be present.
['Jiang|Jinhua|J|', 'Wu|Shenggan|S|', 'Liu|Xinju|X|', 'Wang|Yanhua|Y|', 'An|Xuehua|X|', 'Cai|Leiming|L|', 'Zhao|Xueping|X|']
D000818:Animals D017209:Apoptosis D004625:Embryo, Nonmammalian D052244:Endocrine Disruptors D018507:Gene Expression Regulation, Developmental D006066:Gonads D006540:Herbicides D007113:Immunity, Innate D018384:Oxidative Stress D014052:Toluidines D014158:Transcription, Genetic D015854:Up-Regulation D015027:Zebrafish
Acetochlor Oxidative stress Immunotoxicity Endocrine disruption Zebrafish Cell apoptosis