Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities.
CONTEXT
Origanum (Lamiaceae) has been used in food and pharmaceutical industries.


OBJECTIVE
Isolation and identification of bioactive compounds from Origanum rotundifolium Boiss. and investigation of their antiproliferative and antioxidant activities.


MATERIALS AND METHODS
The aerial part of O. rotundifolium was dried and powdered (1.0 kg ±2.0 g) then extracted with hexane, ethyl acetate, methanol and water. Solvent (3 × 1 L) was used for each extraction for a week at room temperature. The aqueous extract was partitioned with ethyl acetate (3 × 1 L) to yield the water/EtOAc extract subjected to chromatography to isolate the active compounds. The structures of isolated compounds were elucidated by 1 D, 2 D NMR and LC-TOF/MS.


RESULTS
Apigenin (1), ferulic acid (2), vitexin (3), caprolactam (4), rosmarinic acid (5), and globoidnan A (6) were isolated and identified. Globoidnan A (6), vitexin (3), and rosmarinic acid (5) revealed the excellent DPPH• scavenging effect with IC50 values of 22.4, 31.4, 47.2 μM, respectively. Vitexin (3) (IC50 3.6), globoidnan A (6) (IC50 4.6), apigenin (1) (IC50 8.9) and ferulic acid (2) exhibited more ABTS•+ activity than standard Trolox (IC50 13.8 μg/mL). Vitexin (3) revealed the most antiproliferative activity against HeLa, HT29, C6 and Vero cells lines with IC50 values of 35.6, 32.5, 41.6, 46.7 (μM), respectively.


DISCUSSION AND CONCLUSION
Globoidnan A (6) has the most antioxidant effects on all assays. This has to do with the chemical structure of the compound bearing the acidic protons. Vitexin (3) could be a promising anticancer agent.
['Erenler|Ramazan|R|', 'Meral|Bilal|B|', 'Sen|Ozkan|O|', 'Elmastas|Mahfuz|M|', 'Aydin|Ali|A|', 'Eminagaoglu|Ozgur|O|', 'Topcu|Gulacti|G|']
D000818:Animals D000972:Antineoplastic Agents, Phytogenic D000975:Antioxidants D049109:Cell Proliferation D002522:Chlorocebus aethiops D002853:Chromatography, Liquid D019073:HT29 Cells D006367:HeLa Cells D006801:Humans D020128:Inhibitory Concentration 50 D009682:Magnetic Resonance Spectroscopy D013058:Mass Spectrometry D031341:Origanum D035261:Plant Components, Aerial D010936:Plant Extracts D014709:Vero Cells
isolation chromatography Secondary metabolites spectroscopy