The use of design of experiments with multiple responses to determine optimal formulations for in vivo hepatic mRNA delivery.
Although great advances have been made in the delivery of short RNAs by lipid nanoparticles (LNPs), the optimal formulation composition and physicochemical properties of LNPs for long RNA (including mRNA) remain unclear. In the present study, we optimized the lipid composition of liver-targeted mRNA-loaded LNPs that were prepared with pH-sensitive cationic lipids that had been previously designed for siRNA delivery through a two stepped design of experiment (DoE). Multiple responses including physicochemical properties, gene expression, and liver-specificity were analyzed in order, not only to understand the role of each formulation parameter, but also to examine parameters that would be difficult to predict. We found that particle size and the PEG-to-phospholipid (PEG/PL) ratio were additional key factors for liver-specific gene expression in addition to the other formulation factors. The optimized formulation showed a better gene expression compared to other lipid formulations from industry leaders. These findings suggest that a "DoE with multiple responses" approach can be used to predict significant parameters and permit optimized formulations to be prepared more efficiently.
['Hashiba|Akari|A|', 'Toyooka|Manaya|M|', 'Sato|Yusuke|Y|', 'Maeki|Masatoshi|M|', 'Tokeshi|Manabu|M|', 'Harashima|Hideyoshi|H|']
D008055:Lipids D008099:Liver D053758:Nanoparticles D012333:RNA, Messenger D034741:RNA, Small Interfering
Design of Experiment Multiple responses mRNA delivery Lipid nanoparticles Microfluidic device