Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in Pseudomonas aeruginosa.
3',5'-cyclic diguanosine monophosphate (cyclic di-GMP) is a bacterial secondary messenger molecule that regulates many important cellular activities and behaviors, such as motility and biofilm formation. While mass spectrometry protocols for quantitative analyses of intracellular cyclic di-GMP concentrations have been developed, they are time intensive, expensive, low-throughput, and incapable of directly monitoring dynamic changes in vivo. In this protocol, we provide a Pseudomonas aeruginosa-specific detailed methodology to assay the intracellular levels of cyclic di-GMP using biological reporters.
['Borlee|Bradley R|BR|', 'Borlee|Grace I|GI|', 'Martin|Kevin H|KH|', 'Irie|Yasuhiko|Y|']
D006152:Cyclic GMP D015870:Gene Expression D017930:Genes, Reporter D008163:Luminescent Measurements D008856:Microscopy, Fluorescence D011550:Pseudomonas aeruginosa D015290:Second Messenger Systems D014158:Transcription, Genetic
Pseudomonas aeruginosa Cyclic di-GMP Fluorescence assay Luminescence assay c-di-GMP