Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices.
A recent clinical trial proves that autologous olfactory mucosal cell (OMC) transplantation improves locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs respond to the treatment, likely due to the transplantation procedures involving injections of cell suspensions that are associated with cell death, uneven cell distribution, and cell washout. Encapsulating cells in protective hydrogel matrices offers a tissue engineering solution to safely achieve 3D growth of viable transplant cells for implantation into injury sites, to improve regenerative outcomes. It is shown for the first time that canine OMCs (cOMCs) can be propagated with high viability in 3D collagen matrices. Further, a method to incorporate cOMCs pre-labeled with clinical-grade iron oxide nanoparticles into the constructs is described. Intraconstruct labeled cells are visualized using magnetic resonance imaging, offering substantial promise for in vivo tracking of cOMCs delivered in protective matrices.
['Adams|Christopher F|CF|0000-0002-7333-9908', 'Delaney|Alexander M|AM|', 'Carwardine|Darren R|DR|0000-0002-5207-6619', 'Tickle|Jacqueline|J|', 'Granger|Nicolas|N|0000-0002-3036-3908', 'Chari|Divya M|DM|']
D000818:Animals D064987:Cell- and Tissue-Based Therapy D002478:Cells, Cultured D003094:Collagen D004285:Dogs D020100:Hydrogels D008279:Magnetic Resonance Imaging D058185:Magnetite Nanoparticles D023421:Models, Animal D058953:Neural Stem Cells D009831:Olfactory Mucosa D009836:Oligodendroglia D044968:Regenerative Medicine D013119:Spinal Cord Injuries D023822:Tissue Engineering D014182:Transplantation, Autologous
spinal injury hydrogel canine olfactory mucosal cell cell transplantation magnetic nanoparticle