Comparative effect of melatonin and quercetin in counteracting LPS induced oxidative stress in bone marrow mononuclear cells and spleen of Funambulus pennanti.
A majority of cellular diseases, independent of their origin, are characterized by a dramatic increase in Reactive Oxygen Species (ROS) in response to stress. In most cases, the uncontrolled detrimental ROS outburst is difficult to handle for the cellular machinery and eventually leads to cell mortality. In this study, we compare the antioxidant efficacy of quercetin and melatonin to find out a better alternative against lipopolysaccharide (LPS) induced tissue injury by oxidative stress in Funambulus pennanti. Transient exposure to LPS significantly increased ROS generation and lipid peroxidation levels in bone marrow mononuclear cells (MNCs) and spleen which was further corroborated by decreased activities of SOD, CAT and Gpx enzymes. It also downregulate the expression of cellular oxidative stress response proteins Nrf-2 and HO-1 in spleen and decreases the proliferation of bone marrow derived Granulocyte macrophage-colony forming unit cells (GM-CFU). Both melatonin and quercetin pre-treatments rescued these effects, however, our results indicated that the efficacy of melatonin to overcome oxidative stress was significantly better than quercetin. Our findings support the idea that melatonin is a better antioxidant and immunomodulator as compared to other alternatives and perhaps may be employed in the development of effective therapeutics against ROS dominated diseases.
['Rastogi|Shraddha|S|', 'Haldar|Chandana|C|']
D000818:Animals D015415:Biomarkers D001854:Bone Marrow Cells D002374:Catalase D005979:Glutathione Peroxidase D051547:Heme Oxygenase-1 D008070:Lipopolysaccharides D008550:Melatonin D051267:NF-E2-Related Factor 2 D018384:Oxidative Stress D011794:Quercetin D017382:Reactive Oxygen Species D012589:Sciuridae D013154:Spleen D013482:Superoxide Dismutase
Melatonin Quercetin Lipopolysaccharide Immune-system Oxidative-stress