Cyclic Amplification of the Afterglow Luminescent Nanoreporter Enables the Prediction of Anti-cancer Efficiency.
We developed a cyclic amplification method for an organic afterglow nanoreporter for the real-time visualization of self-generated reactive oxygen species (ROS). We promoted semiconducting polymer nanoparticles (PFODBT) as a candidate for emitting near-infrared afterglow luminescence. Introduction of a chemiluminescent substrate (CPPO) into PFODBT (PFODBT@CPPO) resulted in a significant enhancement of afterglow intensity through the dual cyclic amplification pathway involving singlet oxygen (1 O2 ). 1 O2 produced by PFODBT@CPPO induced cancer cell necrosis and promoted the release of damage-related molecular patterns, thereby evoking immunogenic cell death (ICD)-associated immune responses through ROS-based oxidative stress. The afterglow luminescent signals of the nanoreporter were well correlated with light-driven 1 O2 generation and anti-cancer efficiency. This imaging strategy provides a non-invasive tool for predicting the therapeutic outcome that occurs during ROS-mediated cancer therapy.
['Wang|Youjuan|Y|', 'Song|Guosheng|G|', 'Liao|Shiyi|S|', 'Qin|Qiaoqiao|Q|', 'Zhao|Yan|Y|', 'Shi|Linan|L|', 'Guan|Kesong|K|', 'Gong|Xiangyang|X|', 'Wang|Peng|P|', 'Yin|Xia|X|', 'Chen|Qian|Q|', 'Zhang|Xiao-Bing|XB|0000-0002-4010-0028']
D006801:Humans D049449:Luminescence D053758:Nanoparticles D009369:Neoplasms D011108:Polymers D017382:Reactive Oxygen Species D026082:Singlet Oxygen
cyclic amplification afterglow luminescence semiconducting polymer nanoparticles immunogenic cell death molecular imaging