Application of freeze-dried Yarrowia lipolytica biomass in the synthesis of lipophilic antioxidants.
OBJECTIVE
The aim of the study was to evaluate the possibility of using Y. lipolytica biomass as a whole-cell catalyst in the synthesis of lipophilic antioxidants, with the example of esterification of five phenolic acids with 1-butanol.


RESULTS
Freeze-dried Y. lipolytica biomass was successfully applied as a biocatalyst in the synthesis of esters of phenylpropanoic acid derivatives with 75-98% conversion. However, in the case of phenylacetic acid derivatives, results below 10% were obtained. The biological activity of phenolic acid esters was strongly associated with their chemical structures. Butyl 3-(4-hydroxyphenyl)propanoate showed an IC50 value of 19 mg/ml (95 mM) and TEAC value of 0.427. Among the compounds tested, butyl esters of 3-(4-hydroxyphenyl)propanoic and 4-hydroxyphenylacetic acids exhibited the highest antifungal activity.


CONCLUSIONS
Lipophilization of phenolic acids achieved by enzymatic esterification creates prospects for using these compounds as food additives with antioxidant properties in lipid-rich food matrices.
['Zieniuk|Bartłomiej|B|http://orcid.org/0000-0001-6344-8695', 'Wołoszynowska|Małgorzata|M|', 'Białecka-Florjańczyk|Ewa|E|', 'Fabiszewska|Agata|A|']
D020001:1-Butanol D000975:Antioxidants D018533:Biomass D004951:Esterification D005612:Freeze Drying D057927:Hydrophobic and Hydrophilic Interactions D062385:Hydroxybenzoates D025062:Yarrowia
Yarrowia lipolytica Lipophilic antioxidants Candida antarctica lipase B Enzymatic synthesis Phenolic acids esterification