MicroRNA-126-3p suppresses cell proliferation by targeting PIK3R2 in Kaposi's sarcoma cells.
Kaposi's sarcoma is a highly vascular tumor of lymphatic endothelial origin. Many deregulated miRNAs, including miR-126-3p, have been identified in Kaposi's sarcoma tissues. miR-126-3p is the most highly endothelial-specific miRNA that regulates vascular integrity and angiogenesis. In this study, we aimed to determine the effect of miR-126-3p on Kaposi's sarcoma cells through transfection of a miRNA mimic and inhibitor. Moreover, we searched the target gene (PIK3R2) of miR-126-3p using bioinformatics software and further verified PIK3R2 using luciferase reporter assays, Real-time quantitative PCR (qRT-PCR) and western blot. The results demonstrated that miR-126-3p inhibited cell proliferation, arrested cell cycle progression, induced cell apoptosis, and inhibited cell invasion of SLK cells. The bioinformatics analysis and luciferase reporter assay revealed that PIK3R2 mRNA is a direct target of miR-126-3p. Moreover, the level of expression of the PIK3R2 gene was downregulated in SLK cells transfected with miR-126-3p siRNAs. Therefore, our data demonstrated that miR-126-3p is a tumor suppressor miRNA that acts by targeting PIK3R2 in Kaposi's sarcoma cells. These findings contribute to our understanding of the molecular mechanisms underlying Kaposi's sarcoma.
['Wu|Xiu-Juan|XJ|', 'Zhao|Zong-Feng|ZF|', 'Kang|Xiao-Jing|XJ|', 'Wang|Hong-Juan|HJ|', 'Zhao|Juan|J|', 'Pu|Xiong-Ming|XM|']
D020413:3' Untranslated Regions D017209:Apoptosis D015153:Blotting, Western D002453:Cell Cycle D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D015972:Gene Expression Regulation, Neoplastic D006801:Humans D035683:MicroRNAs D019869:Phosphatidylinositol 3-Kinases D034622:RNA Interference D012333:RNA, Messenger D020133:Reverse Transcriptase Polymerase Chain Reaction D012514:Sarcoma, Kaposi
suppress target PIK3R2 miR-126-3p Kaposi’s sarcoma