Improved LC-MS/MS method for the determination of 42 neurologically and metabolically important molecules in urine.
Simultaneous determination of kynurenines, neurotransmitters, pterins and steroids linked to various neurological and metabolic diseases have important diagnostic significance for related pathology and drug monitoring. An improved, sensitive and selective ultra-high performance liquid chromatography coupled to electrospray ionization triple quadrupole mass spectrometric (UHPLC-MS/MS) method, based on our earlier publication, has been proposed for the quantitative measurement of 42 metabolites in human urine. The assay covers a larger number of analytes, uses an advanced, Waters Atlantis T3 chromatographic column and similarly meets the guideline of European Medicines Agency (EMA) on bioanalytical method validation. Analytical performance met all the EMA requirements and the assay covered the relevant clinical concentrations. Linear correlation coefficients were all > 0.998. Intra-day and inter-day accuracy and precision were 87-118%, 81-120% and 2-20%, respectively including the lower limit of quantification (LLOQ). The assay is expected to facilitate the diagnosis and allows drug level monitoring from urine.
['Galla|Zsolt|Z|', 'Rácz|Gábor|G|', 'Grecsó|Nóra|N|', 'Baráth|Ákos|Á|', 'Kósa|Magdolna|M|', 'Bereczki|Csaba|C|', 'Monostori|Péter|P|']
D000328:Adult D015415:Biomarkers D002853:Chromatography, Liquid D006801:Humans D007737:Kynurenine D016014:Linear Models D008875:Middle Aged D018377:Neurotransmitter Agents D011622:Pterins D015203:Reproducibility of Results D012680:Sensitivity and Specificity D053719:Tandem Mass Spectrometry
LC-MS/MS Urine Kynurenine Neurotransmitter Pterin