Stool-Based miR-92a and miR-144* as Noninvasive Biomarkers for Colorectal Cancer Screening.
OBJECTIVES
Current noninvasive screening tests for colorectal cancer (CRC) have insufficient sensitivity. MicroRNA (miRNA) levels in stool have potential as markers for noninvasive screening of CRC. We evaluated the diagnostic value of stool miRNA levels and determined the optimal miRNA subtypes for detecting CRC.


METHODS
Stool samples were collected from 29 patients with CRC and 29 healthy controls. The stool levels of miR-21, miR-92a, miR-200c, miR-144*, miR-135a, miR-135b, miR-106a, and miR-17-3p were determined by real-time quantitative reverse transcription polymerase chain reaction. The sensitivity and specificity of the miRNAs for CRC were determined by receiver operating characteristics analysis.


RESULTS
Among the eight tested miRNAs, the mean stool levels of miR-21, miR-92a, miR-144*, and miR-17-3p differed significantly between the CRC group and the control group (p =0.014, 0.001, <0.001, and 0.008, respectively). The sensitivities and specificities of miR-21, miR-92, miR-144*, and miR-17-3p were 79.3 and 48.3%, 89.7 and 51.7%, 78.6 and 66.7%, and 67.9 and 70.8%, respectively. In a multivariate analysis, miR-92a and miR-144* were significantly associated with the presence of CRC (p = 0.03 and 0.011, respectively).


CONCLUSIONS
The stool levels of miR-92a and miR-144* showed good sensitivity and fair specificity for detection of CRC, and thus may be useful as noninvasive biomarkers for this disease.
['Choi|Hyun Ho|HH|', 'Cho|Young-Seok|YS|', 'Choi|Ji Hye|JH|', 'Kim|Hyung-Keun|HK|', 'Kim|Sung Soo|SS|', 'Chae|Hiun-Suk|HS|']
D000328:Adult D000368:Aged D014408:Biomarkers, Tumor D016022:Case-Control Studies D015179:Colorectal Neoplasms D005243:Feces D005260:Female D005820:Genetic Testing D006801:Humans D008297:Male D035683:MicroRNAs D008875:Middle Aged D060787:Neoplasm Grading D009367:Neoplasm Staging D012372:ROC Curve
Biomarkers MicroRNAs Colorectal neoplasms Early detection of cancer Feces