Propofol affinity to mitochondrial membranes does not alter mitochondrial function.
The molecular mechanisms of hepatotoxicity after propofol anaesthesia have not been fully elucidated, although there is a relation with mitochondrial dysfunction. The action of propofol on mitochondrial hepatic functions in a rat model was evaluated by infusion for 4h with 25 and 62.5mg/kg/h propofol or 3.125ml/kg/h (vehicle). Liver mitochondrial respiratory rates were evaluated as well as mitochondrial transmembrane potential (ΔΨ), calcium fluxes, mitochondrial enzymatic activities (Complex I-V) and oxidative stress biomarkers (superoxide dismutase, catalase, glutathione reductase, glutathione S-transferase, lipid peroxidation and the oxidised/reduced glutathione ratio). Biophysical interactions with membrane models were also performed. The mitochondrial transmembrane potential was decreased and the opening time of the mitochondrial permeability transition pore was slightly reduced for the highest dose. The activity of complex II was stimulated by propofol, which also causes fluctuations on some respiratory parameters, whereas the antioxidant system was affected in a nonspecific manner. Fluorescence quenching studies suggested that propofol is preferably located in deeper regions of the bilayer and has a high affinity to mitochondrial membranes. It is suggested that propofol interacts with liver mitochondrial membranes with mild modification in mitochondrial function.
['Félix|Luís M|LM|', 'Correia|Fernando|F|', 'Pinto|Pedro A|PA|', 'Campos|Sónia P|SP|', 'Fernandes|Telma|T|', 'Videira|Romeu|R|', 'Oliveira|M M|MM|', 'Peixoto|Francisco P|FP|', 'Antunes|Luís M|LM|']
D000818:Animals D015415:Biomarkers D004734:Energy Metabolism D008099:Liver D008297:Male D008928:Mitochondria D051336:Mitochondrial Membranes D018384:Oxidative Stress D010539:Permeability D015742:Propofol D051381:Rats D017208:Rats, Wistar
Propofol Oxidative stress Anaesthetic Bioenergetics Membrane partition Mitochondria