Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution.
A detailed understanding of the paths that stem cells traverse to generate mature progeny is vital for elucidating the mechanisms governing cell fate decisions and tissue homeostasis. Adult stem cells maintain and regenerate multiple mature cell lineages in the olfactory epithelium. Here we integrate single-cell RNA sequencing and robust statistical analyses with in vivo lineage tracing to define a detailed map of the postnatal olfactory epithelium, revealing cell fate potentials and branchpoints in olfactory stem cell lineage trajectories. Olfactory stem cells produce support cells via direct fate conversion in the absence of cell division, and their multipotency at the population level reflects collective unipotent cell fate decisions by single stem cells. We further demonstrate that Wnt signaling regulates stem cell fate by promoting neuronal fate choices. This integrated approach reveals the mechanisms guiding olfactory lineage trajectories and provides a model for deconstructing similar hierarchies in other stem cell niches.
['Fletcher|Russell B|RB|', 'Das|Diya|D|', 'Gadye|Levi|L|', 'Street|Kelly N|KN|', 'Baudhuin|Ariane|A|', 'Wagner|Allon|A|', 'Cole|Michael B|MB|', 'Flores|Quetzal|Q|', 'Choi|Yoon Gi|YG|', 'Yosef|Nir|N|', 'Purdom|Elizabeth|E|', 'Dudoit|Sandrine|S|', 'Risso|Davide|D|', 'Ngai|John|J|']
D053687:Adult Stem Cells D000818:Animals D002455:Cell Division D051379:Mice D008822:Mice, Transgenic D039902:Multipotent Stem Cells D009831:Olfactory Mucosa D060449:Wnt Signaling Pathway
stem cells olfactory epithelium lineage trajectory Wnt HBC branching lineage horizontal basal cell scRNA-seq