The use of a surface active agent in the protection of a fusion protein during bioprocessing.
The bioprocessing of a fusion protein is characterised by low yields and at a series of recovery and purification stages that leads to an overall 90% loss. Much of this apparent loss is due to the denaturation of a protein, missing a vital affinity ligand. However, there is evidence of the protection of degradation products which occurs in the presence of shear plus air/liquid interfaces. This study seeks out to characterise the loss and use ultra-scale-down studies to predict its occurrence and hence shows these may be diminished by the use of protective reagents such as Pluronic F68.
['Blas|Peter|P|0000-0003-2120-1875', 'Tolner|Berend|B|', 'Ward|John|J|0000-0002-4415-5544', 'Chester|Kerry|K|0000-0002-3093-3130', 'Hoare|Mike|M|0000-0001-5215-0746']
D019149:Bioreactors D010843:Pichia D020442:Poloxamer D011489:Protein Denaturation D011993:Recombinant Fusion Proteins D013501:Surface-Active Agents
surface active agent protection fusion protein degradation USD shear device pluronic acid