A fluorometric turn-on aptasensor for mucin 1 based on signal amplification via a hybridization chain reaction and the interaction between a luminescent ruthenium(II) complex and CdZnTeS quantum dots.
A fluorometric method is described for the determination of the tumor biomarker mucin 1 (MUC1). It is based on signal amplification of the hybridization chain reaction (HCR), and the interaction between a luminescent ruthenium(II) complex and CdZnTeS quantum dots (QDs). If MUC1 bind to the biotin-labeled aptamer, it will initiate HCR with hairpins H1 and H2 to form a long-range dsDNA. The long nucleic acid chains are then linked on the surface of streptavidin-modified magnetic microparticles (MMPs) through streptavidin-biotin interaction. The luminescent ruthenium(II) complex is then embedded in the long dsDNA linked to the MMPs. Hence, there is little Ru complex in the supernatant after magnetic separation, and the fluorescence of the CdZnTeS QDs (best measured at excitation/emission wavelengths of 350/530 nm) is only slightly quenched. In the absence of target, the fluorescence of the CdZnTeS QDs is strongly quenched. Fluorescence increases linearly in the 0.2-100 ng·mL-1 MUC1 concentration range, and the LOD is 0.13 ng·mL-1 (at S/N = 3). The method was applied to the determination of MUC1 in spiked human serum samples. Graphical abstract A fluorometric turn-on aptasensor for mucin 1 is described that is based on the interaction between a Ru(II) complex and quantum dots (QDs). The detection system includes biotin-labeled aptamer-H0, hairpins H1 and H2, streptavidin-modified magnetic microparticles (MMPs), Ru(bpy)2(dppx)2+ and CdZnTeS QDs.
['Li|Zheng|Z|', 'Mao|Guobin|G|', 'Du|Mingyuan|M|', 'Tian|Songbai|S|', 'Niu|Longqing|L|', 'Ji|Xinghu|X|', 'He|Zhike|Z|']
D052157:Aptamers, Nucleotide D001483:Base Sequence D015374:Biosensing Techniques D056831:Coordination Complexes D004247:DNA D005456:Fluorescent Dyes D006801:Humans D057230:Limit of Detection D018396:Mucin-1 D021141:Nucleic Acid Amplification Techniques D009693:Nucleic Acid Hybridization D045663:Quantum Dots D012428:Ruthenium D013050:Spectrometry, Fluorescence
Magnetic microparticles Quenching HCR amplification Mucin 1 aptamer