Comparison of the Effect of Exercise on Late-Phase LTP of the Dentate Gyrus and CA1 of Alzheimer's Disease Model.
We investigated the neuroprotective effect of regular treadmill exercise training on long-term memory and its correlate: the late-phase long-term potentiation (L-LTP) and plasticity- and memory-related signaling molecules in the DG and CA1 areas of a rat model of Alzheimer's disease (AD) (i.c.v. infusion of Aβ1-42 peptides, 2 weeks, 250 pmol/day). Testing in the radial arm water maze revealed severe impairment of spatial long-term memory in Aβ-infused sedentary rats but not in exercised Aβ-infused rats. The L-LTP, measured as changes in the field (f)EPSP and in the amplitude of population spike (pspike), was induced by multiple high-frequency stimulation in the CA1 and DG areas of anesthetized rats. The L-LTP of fEPSP in both areas was severely impaired in the sedentary Aβ rats but not in exercised Aβ rats. However, L-LTP of the pspike was severely suppressed in the CA1 area but not in the DG of sedentary Aβ rats. Immunoblot analysis revealed no increase in the levels of phosphorylated (p)-CREB, CaMKIV, and brain-derived neurotrophic factor (BDNF) in both CA1 and DG areas of sedentary Aβ rats during L-LTP, whereas the levels of these molecules were robustly increased in exercised Aβ rats. Impairment of synaptic function may be due to deleterious changes in the molecular signaling cascades that mediate synaptic structural and functional changes. The protective effect of regular exercise can be a promising therapeutic measure for countering or delaying the AD-like pathology.
['Dao|An T|AT|', 'Zagaar|Munder A|MA|', 'Levine|Amber T|AT|', 'Alkadhi|Karim A|KA|']
D000544:Alzheimer Disease D016229:Amyloid beta-Peptides D000818:Animals D019208:Brain-Derived Neurotrophic Factor D056547:CA1 Region, Hippocampal D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4 D017362:Cyclic AMP Response Element-Binding Protein D018891:Dentate Gyrus D004195:Disease Models, Animal D017774:Long-Term Potentiation D008297:Male D018782:Maze Learning D008569:Memory Disorders D010766:Phosphorylation D010805:Physical Conditioning, Animal D017208:Rats, Wistar
Exercise Dentate gyrus Long-term memory L-LTP CA1 area Radial arm water maze fEPSP CaMKIV BDNF Alzheimer’s disease In vivo population spike recording Learning