NEDD 4 binding protein 2-like 1 promotes cancer cell invasion in oral squamous cell carcinoma.
Head and neck cancer, including oral squamous cell carcinoma, is the sixth most common cancer worldwide. Although cancer cell invasion and metastasis are crucial for tumor progression, detailed molecular mechanisms underlying the invasion and metastasis of oral squamous cell carcinoma are unclear. Comparison of transcriptional profiles using a cDNA microarray demonstrated that N4BP2L1, a novel oncogene expressed by neural precursor cells, is involved in oral squamous cell carcinoma. Expression of N4BP2L1 in oral squamous cell carcinoma is regulated by activation of miR-448 and is higher than in normal oral mucosa. Knockdown of N4BP2L1 and upregulation of miR-448 significantly reduced the invasive potential of oral squamous cell carcinoma cells. We studied N4BP2L1 expression in 187 cases of oral squamous cell carcinoma and found its overexpression to be significantly associated with nodal metastasis (P = 0.0155) and poor prognosis (P = 0.0136). Expression of miR-448 was found to be inversely associated with that of N4BP2L1 (P = 0.0019). Cox proportional hazards analysis identified N4BP2L1 expression as an independent predictor of disease-free survival (P = 0.0349). Our results suggest that N4BP2L1 plays an important role in tumor cell invasion in oral squamous cell carcinoma. Further studies on expression of N4BP2L1 may provide new insight into its function and clarify its potential as biomarker in human oral cancer.
['Sasahira|Tomonori|T|', 'Kurihara|Miyako|M|', 'Nishiguchi|Yukiko|Y|', 'Fujiwara|Rina|R|', 'Kirita|Tadaaki|T|', 'Kuniyasu|Hiroki|H|']
D014408:Biomarkers, Tumor D002294:Carcinoma, Squamous Cell D002352:Carrier Proteins D045744:Cell Line, Tumor D002465:Cell Movement D006801:Humans D008207:Lymphatic Metastasis D009062:Mouth Neoplasms D009361:Neoplasm Invasiveness D058953:Neural Stem Cells
Invasion Metastasis N4BP2L1 miR-448 Oral cancer