J-dot targeting of an exported HSP40 in Plasmodium falciparum-infected erythrocytes.
Plasmodium falciparum exports a large number of proteins to its host cell, the mature human erythrocyte, where they are involved in host cell modification. Amongst the proteins trafficked to the host cell, many are heat shock protein (HSP)40 homologues. We previously demonstrated that at least two exported PfHSP40s (referred to as PFE55 and PFA660) localise to mobile structures in the P. falciparum-infected erythrocyte (Kulzer et al., 2010), termed J-dots. The complete molecular content of these structures has not yet been completely resolved, however it is known that they also contain an exported HSP70, PfHSP70x, and are potentially involved in transport of the major cytoadherance ligand, PfEMP1, through the host cell. To understand more about the nature of the association of exported HSP40s with J-dots, here we have studied the signal requirements for recruitment of the proteins to these structures. By expressing various exported GFP chimeras, we can demonstrate that the predicted substrate binding domain is necessary and sufficient for J-dot targeting. This targeting only occurs in human erythrocytes infected with P. falciparum, as it is not conserved when expressing a P. falciparum HSP40 in Plasmodium berghei-infected murine red blood cells, suggesting that J-dots are P. falciparum-specific. This data reveals a new mechanism for targeting of exported proteins to intracellular structures in the P. falciparum-infected erythrocyte.
['Petersen|Wiebke|W|', 'Külzer|Simone|S|', 'Engels|Sonja|S|', 'Zhang|Qi|Q|', 'Ingmundson|Alyssa|A|', 'Rug|Melanie|M|', 'Maier|Alexander G|AG|', 'Przyborski|Jude M|JM|']
D004912:Erythrocytes D049452:Green Fluorescent Proteins D050956:HSP40 Heat-Shock Proteins D018840:HSP70 Heat-Shock Proteins D006801:Humans D018832:Molecular Chaperones D010963:Plasmodium falciparum D014162:Transfection
J-dot HSP40 Erythrocyte Plasmodium falciparum HSP70 Chaperone Malaria Protein targeting