Sphingomonas turrisvirgatae sp. nov., an agar-degrading species isolated from freshwater.
A yellow pigmented and agar-pitting colony was isolated from a water sample obtained from a drainage ditch within a disused system of constructed wetlands. The strain was purified and named MCT13T. This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming, and non-motile strain formed round colonies and grew optimally at pH 7.5±0.2, at 28-30 °C on LB agar, with 0-0.5 % NaCl. The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster. The DNA G+C content was 65.3 %. The only observed ubiquinone was Q10. The major fatty acids included C17 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major polyamine was spermidine. The 16S rRNA gene phylogenetic analysis performed on the whole sequence, showed the closest relative of MCT13T to be Sphingomonas koreensis (98.52 %); however, there are several genotypic and phenotypic differences between the novel isolate and the type strain JSS26T of S. koreensis. On the basis of these results, strain MCT13T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp. nov. is proposed. The type strain is MCT13T (=DSM 105457T=BAC RE RSCIC 7T).
['Thaller|Maria Cristina|MC|', "D'Andrea|Marco Maria|MM|", 'Marmo|Pasquale|P|', 'Civitareale|Cinzia|C|', 'Casu|Francesca|F|', 'Migliore|Luciana|L|']
D000362:Agar D015373:Bacterial Typing Techniques D001482:Base Composition D004269:DNA, Bacterial D005227:Fatty Acids D005618:Fresh Water D007558:Italy D010743:Phospholipids D010802:Phylogeny D012336:RNA, Ribosomal, 16S D017422:Sequence Analysis, DNA D013095:Spermidine D020578:Sphingomonas D014451:Ubiquinone D053833:Wetlands
Sphingomonas turrisvirgatae Alphaproteobacteria agarase-producing new taxon