HIF1A as a major vascular endothelial growth factor regulator: do its polymorphisms have an association with age-related macular degeneration?
BACKGROUND
To investigate the association between age-related macular degeneration (AMD) and the polymorphisms of HIF1A, a major vascular epithelial growth factor regulator under hypoxic conditions. The associations of AMD and polymorphisms of genes CFH, SKIV2L and MYRIP were also studied.


DESIGN
Prospective study.


PARTICIPANTS
Eighty-seven AMD patients and 80 healthy subjects admitted to the Department of Ophthalmology at Pamukkale University Hospital, Denizli, Turkey, were included: 45 (52%) had wet type AMD, and 42 (48%) had dry type AMD.


METHODS
Polymorphisms rs1061170 (CFH), rs429608 (SKIV2L), rs2679798 (MYRIP) and both rs11549465 and rs11549467 (HIF1A) were investigated in DNA isolated from peripheral blood samples of the cases and controls by dye-termination DNA sequencing.


MAIN OUTCOME MEASURES
Genotype distribution of rs1061170 (CFH), rs429608 (SKIV2L), rs2679798 (MYRIP) and both rs11549465 and rs11549467 (HIF1A) in AMD cases and healthy controls; association between genotypes and AMD subtypes.


RESULTS
Given the significant difference between the mean age of case and control groups (72.13 ± 5.77 vs. 62.80 ± 5.22, respectively) (P = .000), subsequent analyses were adjusted for age. We found that having at least one C allele for polymorphism rs1061170 increases AMD risk independent of age (OR = 2.42, 95% confidence interval [CI], 1.22-4.81). The ancestral T allele for polymorphism rs1061170 has a protective effect for AMD (OR = 0.53, 95% CI, 0.34-0.83). No statistically significant difference for distributions of other single nucleotide polymorphisms (SNPs) emerged between patients and healthy subjects.


CONCLUSIONS
No associations appeared between HIF1A SNPs and AMD, which were studied here for the first time; however, polymorphism rs1061170 of the CFH gene is associated with AMD in our population.
['Okur|Volkan|V|', 'Cetin|Ozan|O|', 'Cetin|Ebru|E|', 'Tepeli|Emre|E|', 'Bulgu|Yunus|Y|', 'Yildirim|Cem|C|']
D000368:Aged D017242:Complement Factor H D004265:DNA Helicases D005260:Female D005787:Gene Frequency D005838:Genotype D057092:Geographic Atrophy D006801:Humans D051795:Hypoxia-Inducible Factor 1, alpha Subunit D008297:Male D008875:Middle Aged D016133:Polymerase Chain Reaction D020641:Polymorphism, Single Nucleotide D011446:Prospective Studies D017422:Sequence Analysis, DNA D042461:Vascular Endothelial Growth Factor A D033921:Vesicular Transport Proteins D057135:Wet Macular Degeneration
HIF1A AMD CFH SKIV2L MYRIP