In vitro and 48 weeks in vivo performances of 3D printed porous Fe-30Mn biodegradable scaffolds.
Porous Fe-Mn biodegradable scaffolds fabricated by 3D printing are considered as a promising alternative biomaterial for repairing load-bearing bone defects. However, the mechanical adaptability, the thoughtful in vitro biocompatibility and especially the long-term in vivo osseointegration and biodegradation performances have not been investigated to date. Herein, the porous Fe-30Mn biodegradable scaffolds fabricated by selective laser melting (SLM) had the adjustable elastic modulus ranging from 10.04 GPa to 14.88 GPa by regulating the porosity from 37.89% to 47.17%. In vitro indirect and direct cytotoxicity as well as cell adhesion experiments demonstrated biocompatibility and a large number of cells with stretched filopodia adhered to the scaffolds. 48 weeks in vivo experiments showed that the scaffolds had no harm to liver and kidney, and exhibited long-term in vivo osseointegration performance. Volumes of the scaffolds decreased by 10.1-20.9%, and the retrieved scaffolds showed decreased elastic modulus (decreased by 34.1-42.3%) and yield strength (decreased by 15.8-23.3%) after the 48 weeks in vivo degradation. The Fe-30Mn-femoral condyle complex maintained the same level of stiffness as intact controls during 48 weeks. In summary, the porous Fe-30Mn biodegradable scaffolds fabricated by SLM could be a reliable and practical alternative for repairing load-bearing bone defects.
['Nie|Yong|Y|', 'Chen|Guo|G|', 'Peng|Huabei|H|', 'Tang|Shuo|S|', 'Zhou|Zongke|Z|', 'Pei|Fuxing|F|', 'Shen|Bin|B|']
D001672:Biocompatible Materials D016348:Osseointegration D016062:Porosity D066330:Printing, Three-Dimensional D054457:Tissue Scaffolds D016474:Weight-Bearing
3D printing Porous Fe-30Mn biodegradable scaffolds Load-bearing bone defects Selective laser melting Long-term in vivo performances