GWAS and Post-GWAS High-Resolution Mapping Analyses Identify Strong Novel Candidate Genes Influencing the Fatty Acid Composition of the Longissimus dorsi Muscle in Pigs.
Fatty acid (FA) composition is one of the most important parameters for the assessment of meat quality in pigs. The FA composition in pork can also affect human health. Our aim was to identify quantitative trait loci (QTLs) and positional candidate genes affecting the FA profile of the longissimus dorsi muscle in a large F2 intercross between Landrace and Korean native pigs comprising 1105 F2 progeny by genome-wide association studies (GWAS) and post-GWAS high-resolution mapping analyses. We performed GWAS using the PorcineSNP60K BeadChip and a linear mixed model. Four genome-wide significant QTL regions in SSC8, SSC12, SSC14, and SSC16 were detected (p < 2.53 × 10-7). Several co-localizations of QTLs in SSC12 for oleic acid, linoleic acid, arachidonic acid, monounsaturated FAs, polyunsaturated FAs, and the polyunsaturated/saturated FA ratio were observed. To refine the QTL region in SSC12, a linkage and linkage disequilibrium analysis was applied and could narrow down the critical region to a 0.749 Mb region. Of the genes in this region, GAS7, MYH2, and MYH3 were identified as strong novel candidate genes based on further conditional association analyses. These findings provide a novel insight into the genetic basis of FA composition in pork and could contribute to the improvement of pork quality.
['Lee|Jae-Bong|JB|0000-0001-9331-8146', 'Kang|Yong-Jun|YJ|0000-0001-8949-7831', 'Kim|Sang-Geum|SG|', 'Woo|Jae-Hoon|JH|', 'Shin|Moon-Cheol|MC|', 'Park|Nam-Geon|NG|', 'Yang|Byoung-Chul|BC|', 'Han|Sang-Hyun|SH|', 'Han|Kang-Min|KM|', 'Lim|Hyun-Tae|HT|', 'Ryu|Youn-Chul|YC|', 'Park|Hee-Bok|HB|0000-0002-9418-1898', 'Cho|In-Cheol|IC|0000-0003-3459-1999']
GWAS high-resolution mapping longissimus dorsi muscle human health QTL GAS7 MYH2 MYH3 pork quality fatty acid profile