Antibiofilm and antivirulence properties of chitosan-polypyrrole nanocomposites to Pseudomonas aeruginosa.
Pseudomonas aeruginosa is an opportunistic human pathogen which exhibits its property of pathogenesis due to several factors, including the formation of biofilm and production of several virulence factors. Development of resistance properties against antibiotics leads to the discovery of certain alternative strategies to combat its pathogenesis. In the present study, a highly stable, biocompatible and water soluble nanocomposites (NCs) are synthesized from chitosan (CS) and the polypyrrole (PPy). The resultant chitosan-polypyrrole nanocomposites (CS-PPy NCs) inhibit the establishment of biofilm and also eradicate the preformed matured biofilm formed by P. aeruginosa. CS-PPy NCs inhibit the hemolytic and protease activities of P. aeruginosa. The NCs significantly reduce the production of many virulence factors such as pyocyanin, pyroverdine and rhamnolipid. CS-PPy NCs also suppress the bacterial motility such as swimming and swarming. The present study showed that highly stable CS-PPy NCs act as a potent antibiofilm and antivirulence drug for the treatment of P. aeruginosa infection.
['Khan|Fazlurrahman|F|', 'Manivasagan|Panchanathan|P|', 'Pham|Dung Thuy Nguyen|DTN|', 'Oh|Junghwan|J|', 'Kim|Shin-Kwon|SK|', 'Kim|Young-Mog|YM|']
D000900:Anti-Bacterial Agents D018441:Biofilms D048271:Chitosan D004912:Erythrocytes D006017:Glycolipids D006461:Hemolysis D008826:Microbial Sensitivity Tests D053761:Nanocomposites D010447:Peptide Hydrolases D011108:Polymers D011550:Pseudomonas aeruginosa D011710:Pyocyanine D011758:Pyrroles D037521:Virulence Factors
Antibiofilm Antivirulence Nanocomposites Chitosan Motility Growth