Highly Efficient Cpf1-Mediated Gene Targeting in Mice Following High Concentration Pronuclear Injection.
Cpf1 has emerged as an alternative to the Cas9 RNA-guided nuclease. Here we show that gene targeting rates in mice using Cpf1 can meet, or even surpass, Cas9 targeting rates (approaching 100% targeting), but require higher concentrations of mRNA and guide. We also demonstrate that coinjecting two guides with close targeting sites can result in synergistic genomic cutting, even if one of the guides has minimal cutting activity.
['Watkins-Chow|Dawn E|DE|0000-0002-4355-0868', 'Varshney|Gaurav K|GK|0000-0002-0429-1904', 'Garrett|Lisa J|LJ|', 'Chen|Zelin|Z|', 'Jimenez|Erin A|EA|', 'Rivas|Cecilia|C|', 'Bishop|Kevin S|KS|', 'Sood|Raman|R|0000-0001-5565-662X', 'Harper|Ursula L|UL|', 'Pavan|William J|WJ|0000-0001-8281-5120', 'Burgess|Shawn M|SM|0000-0003-1147-0596']
D045850:Acidaminococcus D000818:Animals D001426:Bacterial Proteins D064113:CRISPR-Cas Systems D004720:Endonucleases D000072669:Gene Editing D018390:Gene Targeting D051379:Mice D017394:RNA, Guide D012333:RNA, Messenger
CRISPR Cpf1 nuclease genome editing mouse