TNF-α-induced p53 activation induces apoptosis in neurological injury.
It was previously confirmed that the apoptotic and necrotic neurons are found during the acute post-traumatic period, suggesting the induction of apoptosis after traumatic brain injury (TBI). To further explore the involvement of apoptotic factors in TBI, an apoptosis antibody array was conducted to measure the alterations of apoptotic factors in rat brain cortex after TBI. As a result, the Neurological Severity Scale (NSS) scores after TBI were increased, and the cell morphology of the brain cortex was destructed with increased neuronal apoptosis. Furthermore, the caspase-3 activity was increased, and the apoptotic-related factors TNF-α and p53 were up-regulated in the brain cortex. More importantly, in vitro experiments demonstrated that down-regulation of TNF-α in oxygen-glucose deprivation/reoxygenation (OGD/R) cells increased cell viability and decreased apoptosis and the p53 expression. These results suggested the involvement of TNF-α-induced apoptotic signalling pathway by activating p53 in the molecular mechanism of neurological injury.
['Shao|Xuefei|X|', 'Yang|Xiping|X|', 'Shen|Jun|J|', 'Chen|Sansong|S|', 'Jiang|Xiaochun|X|', 'Wang|Qifu|Q|', 'Di|Qiang|Q|0000-0001-8857-7805']
D000818:Animals D000906:Antibodies D017209:Apoptosis D000070642:Brain Injuries, Traumatic D053148:Caspase 3 D002470:Cell Survival D015536:Down-Regulation D005947:Glucose D009474:Neurons D010100:Oxygen D034622:RNA Interference D017207:Rats, Sprague-Dawley D015398:Signal Transduction D014409:Tumor Necrosis Factor-alpha D016159:Tumor Suppressor Protein p53
p53 apoptosis traumatic brain injury TNF-α apoptotic signalling pathway