Expression and Purification of JAK1 and SOCS1 for Structural and Biochemical Studies.
Interferon gamma (IFNγ) is a potent inflammatory and immune cytokine. IFNγ signals via the interferon gamma receptor (IFNGR), which is constitutively bound to Janus Kinase (JAK) 1 and JAK2 via its intracellular domain. These two JAK proteins then initiate the inflammatory signaling cascade. The most potent inhibitor of IFNγ signaling is Suppressor of Cytokine Signaling 1 (SOCS1). SOCS1 negatively regulates IFNγ signaling pathway (and other pathways) by directly inhibiting JAKs. Here, we describe a protocol for the recombinant production and purification of the JAK1 kinase domain and its inhibitor SOCS1, for structural and biochemical studies.
['Liau|Nicholas P D|NPD|', 'Babon|Jeffrey J|JJ|']
D000818:Animals D016367:Baculoviridae D002478:Cells, Cultured D004926:Escherichia coli D006801:Humans D053613:Janus Kinase 1 D011994:Recombinant Proteins D061987:Sf9 Cells D015398:Signal Transduction D000071222:Suppressor of Cytokine Signaling 1 Protein
Janus Kinase (JAK) Protein expression Protein purification Suppressor of cytokine signaling (SOCS)