Antimicrobial effects of Lactobacillus plantarum and Lactobacillus acidophilus against multidrug-resistant enteroaggregative Escherichia coli.
The in vitro and in vivo antimicrobial effects of Lactobacillus plantarum and Lactobacillus acidophilus were evaluated individually and synergistically against multidrug-resistant enteroaggregative Escherichia coli (MDR-EAEC). In vitro evaluation of each probiotic strain when co-cultured with MDR-EAEC isolates revealed a reduction in MDR-EAEC counts (eosin-methylene blue agar) in a dose- and time-dependent manner: probiotics at a dose rate of 10(10) CFU inhibited MDR-EAEC isolates at 72 h post-inoculation (PI), whereas at lower concentrations (10(8) and 10(9) CFU) MDR-EAEC isolates were inhibited at 96 h PI. The synergistic antimicrobial effect of both probiotic strains (each at 10(10) CFU) was highly significant (P < 0.01) and inhibited the growth of MDR-EAEC isolates at 24 h PI. For in vivo evaluation, weaned mice were fed orally with 10(7) CFU of MDR-EAEC. At Day 3 post-infection, treated mice were fed orally with the probiotic strains (each at 10(10) CFU). Compared with the control, post-treatment a significant (P < 0.01) reduction in MDR-EAEC counts was observed in faeces by Day 2 and in intestinal tissues of treated mice by Days 3 and 4 as evidenced by plate count (mean 2.71 log and 2.27 log, respectively) and real-time PCR (mean 1.62 log and 1.57 log, respectively) methods. Histopathologically, comparatively mild changes were observed in the ileum and colon from Days 3 to 5 post-treatment with probiotics; however, from Day 6 the changes were regenerative or normal. These observations suggest that these probiotic strains can serve as alternative therapeutics against MDR-EAEC-associated infections in humans and animals.
['Kumar|Manesh|M|', 'Dhaka|Pankaj|P|', 'Vijay|Deepthi|D|', 'Vergis|Jess|J|', 'Mohan|Vysakh|V|', 'Kumar|Ashok|A|', 'Kurkure|Nitin V|NV|', 'Barbuddhe|Sukhadeo B|SB|', 'Malik|S V S|SV|', 'Rawool|Deepak B|DB|']
D000818:Animals D000831:Animals, Newborn D000898:Antibiosis D058491:Bacterial Load D015169:Colony Count, Microbial D004926:Escherichia coli D004927:Escherichia coli Infections D007779:Lactobacillus acidophilus D048191:Lactobacillus plantarum D008297:Male D051379:Mice D019936:Probiotics D060888:Real-Time Polymerase Chain Reaction D013997:Time Factors D016896:Treatment Outcome
Lactobacillus plantarum Lactobacillus acidophilus Enteroaggregative Escherichia coli Probiotics Drug resistance