Tools and Techniques for Wt1-Based Lineage Tracing.
The spatiotemporal expression pattern of Wt1 has been extensively studied in a number of animal models to establish its function and the developmental fate of the cells expressing this gene. In this chapter, we review the available animal models for Wt1-expressing cell lineage analysis, including direct Wt1 expression reporters and systems for permanent Wt1 lineage tracing. We describe the presently used constitutive or inducible genetic lineage tracing approaches based on the Cre/loxP system utilizing Cre recombinase expression under control of a Wt1 promoter.To make these systems accessible, we provide laboratory protocols that include dissection and processing of the tissues for immunofluorescence and histopathological analysis of the lineage-labeled Wt1-derived cells within the embryo/tissue context.
['Wilm|Bettina|B|', 'Muñoz-Chapuli|Ramon|R|']
D000818:Animals D019070:Cell Lineage D058948:Cell Tracking D017930:Genes, Reporter D019426:Integrases D051379:Mice D008822:Mice, Transgenic D023421:Models, Animal D011401:Promoter Regions, Genetic D012097:Repressor Proteins D013629:Tamoxifen D025721:WT1 Proteins
Wt1 Cre/loxP Cell lineage tracing Cre-ERT2 Inducible Cre recombinase Rosa26 reporter mice Tamoxifen