Piperine mediates LPS induced inflammatory and catabolic effects in rat intervertebral disc.
Piperine is an exact of the active phenolic component from Black pepper. It has been reported to have many biological activities including anti-oxidant, anti-inflammatory and anti-tumor effects. Intervertebral disc degeneration (IDD) is a degenerative disease closely relate to inflammation of nucleus pulposus (NP) cells. This study aimed to assess the anti-inflammatory and anti-catabolic effects of piperine in rat intervertebral disc using in vitro and ex vivo analyzes. We demonstrated that piperine could inhibit LPS induced expression and production of inflammatory factors and catabolic proteases in NP cells culture model. It significantly inhibited multiple inflammatory factors and oxidative stress-associated genes (IL-1β, TNF-α, IL-6, iNOS), MMPs (MMP-3, MMP-13), ADAMTS (ADAMTS-4, ADAMTS-5) mRNA expression and NO production in a concentration-dependent manner. Moreover, piperine could reverse the LPS-induced inhibition of gene expression of aggrecan and collagen-II. Histologic and dimethylmethylene blue analysis indicated piperine could also against LPS induced proteoglycan (PG) depletion in a rat intervertebral disc culture model. Western blot results showed that piperine inhibited the LPS-mediated phosphorylation of JNK and activation of NF-κB. Finally, our results demonstrated the ability of piperine to antagonize LPS-mediated inflammation of NP cells and suppression of PG in rat intervertebral disc, suggesting a potential agent for treatment of IDD in future.
['Li|Yan|Y|', 'Li|Kang|K|', 'Hu|Yiqin|Y|', 'Xu|Bo|B|', 'Zhao|Jie|J|']
D053674:Aggrecans D000470:Alkaloids D000818:Animals D000893:Anti-Inflammatory Agents D052117:Benzodioxoles D002470:Cell Survival D002478:Cells, Cultured D024043:Collagen Type II D019610:Cytoprotection D004305:Dose-Response Relationship, Drug D005786:Gene Expression Regulation D018836:Inflammation Mediators D007403:Intervertebral Disc D055959:Intervertebral Disc Degeneration D048031:JNK Mitogen-Activated Protein Kinases D008070:Lipopolysaccharides D008666:Metalloendopeptidases D016328:NF-kappa B D009924:Organ Culture Techniques D010766:Phosphorylation D010880:Piperidines D053284:Polyunsaturated Alkamides D012333:RNA, Messenger D017207:Rats, Sprague-Dawley D015398:Signal Transduction D013997:Time Factors
Piperine intervertebral disc degeneration inflammation nucleus pulposus cells