Magnetic resonance imaging of tumor with a self-traceable polymer conjugated with an antibody fragment.
A (13)C-enriched phosphorylcholine polymer ((13)C-PMPC) as a self-traceable MR (magnetic resonance) tag was conjugated with a fragment (scFv) of Herceptin, a clinical antibody against antigen Her2. When injected in model mice bearing Her2(+) (gastric) and Her2(-) (pancreatic) tumors, the antibody-tag conjugate (13)C-PMPC-scFv selectively accumulated in the Her2(+) tumor with a rapid build-up/decay (accumulation/clearance) profile and, with the use of the (1)H-(13)C double-resonance (heteronuclear correlation) technique, the Her2(+) gastric tumor was clearly MR imaged.
['Yamada|Hisatsugu|H|', 'Hasegawa|Yoshinori|Y|', 'Suzuki|Yuki|Y|', 'Imai|Hirohiko|H|', 'Matsuda|Tetsuya|T|', 'Kimura|Yu|Y|', 'Toshimitsu|Akio|A|', 'Aoyama|Yasuhiro|Y|', 'Kondo|Teruyuki|T|']
D000818:Animals D002247:Carbon Isotopes D045744:Cell Line, Tumor D005260:Female D006801:Humans D018796:Immunoconjugates D008279:Magnetic Resonance Imaging D051379:Mice D008807:Mice, Inbred BALB C D008819:Mice, Nude D010190:Pancreatic Neoplasms D010767:Phosphorylcholine D011109:Polymethacrylic Acids D018719:Receptor, ErbB-2 D057127:Single-Chain Antibodies D013274:Stomach Neoplasms
Self-traceable polymer Active targeting Anti-Her2 antibody MRI Tumor imaging