Vitamin B6 and homocysteine levels in carbamazepine treated epilepsy of Khyber Pakhtunkhwa.
OBJECTIVES
The study focused on the plasma levels of vitamin B6 and homocysteine in different genotypes of MTHFR (C677T, A1298C) and GABRG2 (C588T, C315T) genes in carbamazepine resistant epilepsy in the population of Khyber Pakhtunkhwa.


METHODOLOGY
Patients who were possible candidates for carbamazepine therapy were followed for six months for their seizure control. Plasma levels of vitamin B6 and homocysteine were determined using immunoassay based techniques at baseline and after six months. MTHFR (C677T, A1298C) and GABRG2 (C588T, C315T) genes were genotyped using restriction fragment length polymorphisms. Seizure control during therapy was recorded on a standardized proforma.


RESULTS
Low vitamin B6 levels and hyperhomocysteinemia were found in 61.7% of resistant patients (n=34). Resistant patients had the following frequencies of variant genotypes (677CT=38.1% and 677TT=24.4%; 1298AC=42.2% and 1298CC=26.1%; 588CT= 47.6% and 315TT= 33.3%) of MTHFR (C677T and A1298C) and GABRG2 (C588T and C315T) genes. A significant decline in vitamin B6 (P<0.0001) and hyperhomocysteinemia were found in variant genotypes of MTHFR (C677T, A1298C) and GABRG2 (C588T, C315T) genes.


CONCLUSION
Following six months of carbamazepine of therapy in heterozygous variant genotypes of MTHFR (677CT and 1298AC) and GABRG2 (588CT and 315CT) genes, we observed a significant fall in vitamin B6 levels and hyperhomocysteinemia.
['Shakir|Shakirullah|S|', 'Ali|Niaz|N|', 'Udin|Zia|Z|', 'Nazish|Haleema|H|', 'Nabi|Muhammad|M|']
D000293:Adolescent D000328:Adult D000927:Anticonvulsants D002220:Carbamazepine D002648:Child D002675:Child, Preschool D000069279:Drug Resistant Epilepsy D004827:Epilepsy D005260:Female D005838:Genotype D006710:Homocysteine D006801:Humans D007223:Infant D008297:Male D042965:Methylenetetrahydrofolate Reductase (NADPH2) D010154:Pakistan D020641:Polymorphism, Single Nucleotide D011963:Receptors, GABA-A D025101:Vitamin B 6 D055815:Young Adult
vitamin B6 homocysteine Carbamazepine seizure control RFLP epileptics