The AhR is involved in the regulation of LoVo cell proliferation through cell cycle-associated proteins.
Some ingredients in foods can activate the aryl hydrocarbon receptor (AhR) and arrest cell proliferation. In this study, we hypothesized that 6-formylindolo [3, 2-b] carbazole (FICZ) arrests the cell cycle in LoVo cells (a colon cancer line) through the AhR. The AhR agonist FICZ and the AhR antagonist CH223191 were used to treat LoVo cells. Real-time PCR and Western blot analyses were performed to detect the expression of the AhR, CYP1A1, CDK4, cyclinD1, cyclin E, CDK2, P27, and pRb. The distribution and activation of the AhR were detected with immunofluorescence. A 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and flow cytometric analysis were performed to measure cell viability, cell cycle stage, and apoptosis. Our results show that FICZ inhibited LoVo cell proliferation by inducing G1 cell cycle arrest but had no effect on epithelial apoptosis. Further analysis found that FICZ downregulated cyclinD1 and upregulated p27 expression to arrest Rb phosphorylation. The downregulation of cyclinD1 and upregulation of p27 were abolished by co-treatment with CH223191. We conclude that the AhR, when activated by FICZ (an endogenous AhR ligand), can arrest the cell cycle and block LoVo cell proliferation.
['Yin|Jiuheng|J|', 'Sheng|Baifa|B|', 'Han|Bin|B|', 'Pu|Aimin|A|', 'Yang|Kunqiu|K|', 'Li|Ping|P|', 'Wang|Qimeng|Q|', 'Xiao|Weidong|W|', 'Yang|Hua|H|']
D017209:Apoptosis D002227:Carbazoles D018797:Cell Cycle Proteins D045744:Cell Line, Tumor D049109:Cell Proliferation D002470:Cell Survival D019938:Cyclin D1 D019363:Cytochrome P-450 CYP1A1 D059585:G1 Phase Cell Cycle Checkpoints D006801:Humans D008565:Membrane Proteins D010766:Phosphorylation D018336:Receptors, Aryl Hydrocarbon D015398:Signal Transduction
proliferation aryl hydrocarbon receptor (AhR) 6-formylindolo (3, 2-b) carbazole (FICZ) G1 arrest