Overlap between eQTL and QTL associated with production traits and fertility in dairy cattle.
BACKGROUND
Identifying causative mutations or genes through which quantitative trait loci (QTL) act has proven very difficult. Using information such as gene expression may help to identify genes and mutations underlying QTL. Our objective was to identify regions associated both with production traits or fertility and with gene expression, in dairy cattle. We used three different approaches to discover QTL that are also expression QTL (eQTL): 1) estimate the correlation between local genomic estimated breeding values (GEBV) and gene expression, 2) investigate whether the 300 intervals explaining most genetic variance for a trait contain more eQTL than 300 randomly selected intervals, and 3) a colocalisation analysis. Phenotypes and genotypes up to sequence level of 35,775 dairy bulls and cows were used for QTL mapping, and gene expression and genotypes of 131 cows were used to identify eQTL.


RESULTS
With all three approaches, we identified some overlap between eQTL and QTL, though the majority of QTL in our dataset did not seem to be eQTL. The most significant associations between QTL and eQTL were found for intervals on chromosome 18, where local GEBV for all traits showed a strong association with the expression of the FUK and DDX19B. Intervals whose local GEBV for a trait correlated highly significantly with the expression of a nearby gene explained only a very small part of the genetic variance for that trait. It is likely that part of these correlations were due to linkage disequilibrium (LD) in the interval. While the 300 intervals explaining most genetic variance explained most of the GEBV variance, they contained only slightly more eQTL than 300 randomly selected intervals that explained a minimal portion of the GEBV variance. Furthermore, some variants showed a high colocalisation probability, but this was only the case for few variants.


CONCLUSIONS
Several reasons may have contributed to the low level of overlap between QTL and eQTL detected in our study, including a lack of power in the eQTL study and long-range LD making it difficult to separate QTL and eQTL. Furthermore, it may be that eQTL explain only a small fraction of QTL.
['van den Berg|I|I|http://orcid.org/0000-0002-9292-8636', 'Hayes|B J|BJ|', 'Chamberlain|A J|AJ|', 'Goddard|M E|ME|']
D000818:Animals D002417:Cattle D003612:Dairying D005298:Fertility D014644:Genetic Variation D055106:Genome-Wide Association Study D040641:Quantitative Trait Loci
eQTL QTL Dairy cattle Quantitative traits Gene expression