Quantitative Evaluation of Hepatic Microvascular Perfusion after Ischemia-Reperfusion Injury in Rabbits by Contrast-Enhanced Ultrasound Perfusion Imaging.
The aim of this study was to evaluate microvascular perfusion after liver ischemia-reperfusion injury (IRI) in rabbits using the "flash-replenishment" method of contrast-enhanced ultrasound (CEUS) perfusion imaging. Twenty-eight rabbits underwent either 30, 60 or 90 min of ischemia and 120 min of reperfusion. CEUS perfusion imaging was performed using the "flash-replenishment" model, and hepatic microvascular perfusion parameters, including peak intensity (PI), area under the curve (AUC), and hepatic artery-to-vein transit time (HA-HVTT), were calculated. Prolonged ischemia upregulated intracellular adhesion molecule-1 (ICAM-1), alanine transaminase (ALT) and aspartate transaminase (AST) levels. Longer ischemia decreased PI and AUC, but increased HA-HVTT. The perfusion parameters were significantly correlated with Suzuki's pathology scores and ALT and AST levels. The "flash-replenishment" method of CEUS perfusion imaging is an accurate and non-invasive method for evaluating hepatic microvascular perfusion and provides a valuable experimental basis for early prediction of liver IRI damage after liver transplantation or liver resection.
['Xie|Fang|F|', 'Fei|Xiang|X|', 'Zhang|Ming-Bo|MB|', 'Zhang|Yan|Y|', 'Wang|Hong-Wei|HW|', 'Tang|Jie|J|', 'Tang|Wen-Bo|WB|', 'Luo|Yu-Kun|YK|']
D000818:Animals D003287:Contrast Media D004195:Disease Models, Animal D005069:Evaluation Studies as Topic D007089:Image Enhancement D008099:Liver D008107:Liver Diseases D008833:Microcirculation D011817:Rabbits D015427:Reperfusion Injury D014463:Ultrasonography
Hepatic microvascular perfusion Contrast-enhanced ultrasound Ischemia–reperfusion injury