SIRT1 suppresses adipogenesis by activating Wnt/β-catenin signaling in vivo and in vitro.
Sirtuin 1 (SIRT1) regulates adipocyte and osteoblast differentiation. However, the underlying mechanism should be investigated. This study revealed that SIRT1 acts as a crucial repressor of adipogenesis. RNA-interference-mediated SIRT1 knockdown or genetic ablation enhances adipogenic potential, whereas SIRT1 overexpression inhibits adipogenesis in mesenchymal stem cells (MSCs). SIRT1 also deacetylates the histones of sFRP1, sFRP2, and Dact1 promoters; inhibits the mRNA expression of sFRP1, sFRP2, and Dact1; activates Wnt signaling pathways; and suppresses adipogenesis. SIRT1 deacetylates β-catenin to promote its accumulation in the nucleus and thus induces the transcription of genes that block MSC adipogenesis. In mice, the partial absence of SIRT1 promotes the formation of white adipose tissues without affecting the development of the body of mice. Our study described the regulatory role of SIRT1 in Wnt signaling and proposed a regulatory mechanism of adipogenesis.
['Zhou|Yuanfei|Y|', 'Song|Tongxing|T|', 'Peng|Jie|J|', 'Zhou|Zheng|Z|', 'Wei|Hongkui|H|', 'Zhou|Rui|R|', 'Jiang|Siwen|S|', 'Peng|Jian|J|']
D000107:Acetylation D050156:Adipogenesis D000818:Animals D002454:Cell Differentiation D002460:Cell Line D055785:Gene Knockdown Techniques D006657:Histones D066298:In Vitro Techniques D059630:Mesenchymal Stem Cells D051379:Mice D056564:Sirtuin 1 D060449:Wnt Signaling Pathway
SIRT1 adipogenesis β-catenin mesenchymal stem cells Wnt signaling antagonists