Iron-regulated gene ireA in avian pathogenic Escherichia coli participates in adhesion and stress-resistance.
BACKGROUND
Avian pathogenic Escherichia coli (APEC) causes avian colibacillosis, which results in economic and welfare costs in the poultry industry worldwide. The pathogenesis of avian pathogenic E. coli strains is not well defined. Here, the function of an outer membrane protein encoded by the ireA gene of avian pathogenic E. coli strain DE205B was investigated.


RESULTS
The ireA gene was distributed in 32.9 % (46/140) of tested E. coli strains, with high percentages in the phylogenetic ECOR groups B2 (58.8 %, 10/17) and D (55.9 %, 19/34). The gene expression level of ireA of APEC strain DE205B in high Fe M9 media was 1.8 times higher (P < 0.05) than that in low Fe M9 media. An ireA deletion mutant and complementary strain were constructed. Compared with the wild-type strain DE205B, the expression of most ferric uptake genes in the ireA deletion mutant were significantly upregulated (P < 0.05). The adhesion ability of the ireA deletion mutant to DF-1 cells was significantly decreased. The survival rate of ireA deletion mutant was reduced 21.17 % (P < 0.01), 25.42 (P < 0.05) and 70.0 % (P < 0.01) under alkali, high osmolarity, and low temperature (4 °C) conditions, respectively, compared with the wild-type strain.


CONCLUSIONS
The results suggested that the protein encoded by the iron-regulated gene ireA has roles in adhesion and stress resistance in avian pathogenic E. coli.
['Li|Yaxin|Y|', 'Dai|Jianjun|J|', 'Zhuge|Xiangkai|X|', 'Wang|Haojin|H|', 'Hu|Lin|L|', 'Ren|Jianluan|J|', 'Chen|Ling|L|', 'Li|Dezhi|D|', 'Tang|Fang|F|']
D000818:Animals D001422:Bacterial Adhesion D004372:Ducks D004926:Escherichia coli D029968:Escherichia coli Proteins D015964:Gene Expression Regulation, Bacterial D009154:Mutation D011201:Poultry Diseases D013312:Stress, Physiological
IreA Adhesion APEC Stress resistance Virulence