A bacterial homologue of the human iron exporter ferroportin.
A bacterial homologue of the human iron exporter ferroportin found in the predatory Gram-negative bacterium Bdellovibrio bacteriovorus has been investigated. Molecular modelling, expression in recombinant form and iron binding and transport assays demonstrate that B. bacteriovorus ferroportin (bdFpn) is indeed an orthologue of human ferroportin. Key residues corresponding to those essential for iron binding and transport in human ferroportin are conserved in the bacterial homologue and are predicted to be correctly clustered in the central cavity of the protein. Mutation of these residues grossly affects the iron binding and transport ability of bdFpn.
['Bonaccorsi di Patti|Maria Carmela|MC|', 'Polticelli|Fabio|F|', 'Tortosa|Valentina|V|', 'Furbetta|Pier Antonio|PA|', 'Musci|Giovanni|G|']
D000595:Amino Acid Sequence D001426:Bacterial Proteins D001501:Bdellovibrio D027682:Cation Transport Proteins D017124:Conserved Sequence D006801:Humans D007501:Iron D008958:Models, Molecular D008969:Molecular Sequence Data D016297:Mutagenesis, Site-Directed D009154:Mutation D011487:Protein Conformation D021381:Protein Transport D017386:Sequence Homology, Amino Acid
Iron Ferroportin Bdellovibrio bacteriovorus Major facilitator superfamily