Correlation between selected angiogenic markers and prognosis in pediatric adrenocortical tumors: Angiogenic markers and prognosis in pediatric ACTs.
BACKGROUND/PURPOSE
Pediatric adrenocortical tumor (ACT) remains a challenging disease. Tumor weight and disease stage are still the most used indicators to prognosis and guidance of clinical decisions. Histology has not added meaningful data for risk stratification and management. ACT is metabolically active, highly vascularized, locally invasive and has the propensity to produce distant metastasis. Our objective was to correlate the expression of vascular endothelial growth factor (VEGF) and intratumoral microvessel density (MVD) with clinical and prognostic aspects in pediatric ACT.


PROCEDURE
In 27 tumors, immunohistochemical expression of VEGF, CD105 (endoglin) and CD34 was analyzed. MVD was determined by CD34 and CD105 antibodies. MVD and VEGF expression was correlated with clinical characteristics and outcome. Normal pediatric glands were used as controls.


RESULTS
Endoglin MVD was significantly higher and CD34 MVD was significantly lower in ACT than control. The VEGF expression did not differ between groups. Cytoplasmic staining for endoglin was correlated with hypertension in ACT. Endoglin MVD greater than 1 mv/field, CD34 MVD less than 32 mv/field and VEGF expression levels above 4.8% were associated with clinical and biological indicators of poor prognosis.


CONCLUSIONS
Endoglin and CD34 MVD values are potential histological markers to refine the histologic classification of pediatric ACT.
['Dias|André Ivan Bradley dos Santos|AI|', 'Fachin|Camila Girardi|CG|', 'Avó|Lucimar Retto Silva|LR|', 'Frazão|Caio Vinicius Gonçalves|CV|', 'Caran|Eliana Maria Monteiro|EM|', 'Schettini|Sérgio Tomaz|ST|', 'Alves|Maria Teresa Seixas|MT|', 'Ribeiro|Raul C|RC|', 'Abib|Simone de Campos Vieira|Sde C|']
D000293:Adolescent D000302:Adrenal Cortex D000306:Adrenal Cortex Neoplasms D018268:Adrenocortical Carcinoma D015703:Antigens, CD D018952:Antigens, CD34 D014408:Biomarkers, Tumor D016022:Case-Control Studies D002648:Child D002675:Child, Preschool D000071063:Endoglin D005260:Female D005500:Follow-Up Studies D006801:Humans D007150:Immunohistochemistry D008297:Male D055806:Microvessels D009389:Neovascularization, Pathologic D011379:Prognosis D011956:Receptors, Cell Surface D012189:Retrospective Studies D016019:Survival Analysis D042461:Vascular Endothelial Growth Factor A
Prognosis Adrenocortical tumor Angiogenesis and children Immunohistochemistry