Secoisolariciresinol diglucoside rich extract of L. usitatissimum prevents diabetic colon cancer through inhibition of CDK4.
BACKGROUND
There is increased risk of colon cancer in both men and women having diabetes. The objective of the study was to evaluate the role of Secoisolariciresinol diglucoside rich extract(SRE) of L.usissatisimum(flaxseed) in colon cancer associated with type 2 diabetes mellitus.


MATERIAL AND METHODS
Diabetes was induced by administering high fat diet with low dose streptozotocin model. After 6 weeks, diabetes was confirmed and 1,2 dimethylhydrazine(25mg/kg, sc) weekly administration was from 6th to 18th weeks. Rats were treated with the SRE(500mg/kg) orally from 6th to 24th week. After 24 weeks, various biochemical and enzymatic parameters were estimated. Animals were sacrificed and colon tissue was separated and subjected to analysis of histopathological, PCNA studies and mRNA expression of CDK4.


RESULTS
Disease control rats depicted hyperglycaemia, hyperinsulinaemia, elevated pro-inflammatory cytokines and cancer biomarker levels, and marked presence of proliferating cells. Treatment with SRE controlled hyperglycaemia, hyperinsulinaemia, reduced pro-inflammatory cytokines and cancer biomarker levels, and decreased no. of proliferating cells. We found that disease control rats depicted over expression of CDK4 mRNA levels which were reduced by SRE treatment.


CONCLUSIONS
SRE of L. usitatissimum exhibited chemopreventive effect in colon cancer associated with type 2 diabetes mellitus which might be mediated through inhibition of CDK4.
['Shah|Niyati R|NR|', 'Patel|Bhoomika M|BM|']
D000818:Animals D014408:Biomarkers, Tumor D002072:Butylene Glycols D049109:Cell Proliferation D003110:Colonic Neoplasms D051358:Cyclin-Dependent Kinase 4 D016207:Cytokines D003924:Diabetes Mellitus, Type 2 D005260:Female D019597:Flax D015972:Gene Expression Regulation, Neoplastic D005951:Glucose Tolerance Test D005960:Glucosides D006943:Hyperglycemia D018836:Inflammation Mediators D008297:Male D064209:Phytochemicals D010936:Plant Extracts D018809:Proliferating Cell Nuclear Antigen D012333:RNA, Messenger D017207:Rats, Sprague-Dawley
Secoisolariciresinol diglucoside L. usitatissimum CDK4 Colon cancer associated with type 2 diabetes mellitus DMH