Feruloyl esterase immobilization in mesoporous silica particles and characterization in hydrolysis and transesterification.
BACKGROUND
Enzymes display high reactivity and selectivity under natural conditions, but may suffer from decreased efficiency in industrial applications. A strategy to address this limitation is to immobilize the enzyme. Mesoporous silica materials offer unique properties as an immobilization support, such as high surface area and tunable pore size.


RESULTS
The performance of a commercially available feruloyl esterase, E-FAERU, immobilized on mesoporous silica by physical adsorption was evaluated for its transesterification ability. We optimized the immobilization conditions by varying the support pore size, the immobilization buffer and its pH. Maximum loading and maximum activity were achieved at different pHs (4.0 and 6.0 respectively). Selectivity, shown by the transesterification/hydrolysis products molar ratio, varied more than 3-fold depending on the reaction buffer used and its pH. Under all conditions studied, hydrolysis was the dominant activity of the enzyme. pH and water content had the greatest influence on the enzyme selectivity and activity. Determined kinetic parameters of the enzyme were obtained and showed that Km was not affected by the immobilization but kcat was reduced 10-fold when comparing the free and immobilized enzymes. Thermal and pH stabilities as well as the reusability were investigated. The immobilized biocatalyst retained more than 20% of its activity after ten cycles of transesterification reaction.


CONCLUSIONS
These results indicate that this enzyme is more suited for hydrolysis reactions than transesterification despite good reusability. Furthermore, it was found that the immobilization conditions are crucial for optimal enzyme activity as they can alter the enzyme performance.
['Bonzom|Cyrielle|C|', 'Schild|Laura|L|', 'Gustafsson|Hanna|H|', 'Olsson|Lisbeth|L|']
D002021:Buffers D002265:Carboxylic Ester Hydrolases D004795:Enzyme Stability D004800:Enzymes, Immobilized D004951:Esterification D006863:Hydrogen-Ion Concentration D006868:Hydrolysis D016062:Porosity D012822:Silicon Dioxide
Mesoporous silica E-FAERU Kinetic parameters Enzyme reusability Enzyme stability Feruloyl esterase selectivity