Anti-citrullinated heat shock protein 90 antibodies identified in bronchoalveolar lavage fluid are a marker of lung-specific immune responses.
Previous work has demonstrated a correlation between serum anti-citrullinated HSP90 antibodies and rheumatoid arthritis-associated interstitial lung disease (RA-ILD). To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. 9/21 RA-derived BALF specimens demonstrated IgG and/or IgA antibodies targeting citrullinated HSP90 proteins/peptides, highlighting disease specific responses (with a predilection for RA-ILD) that did not occur in IPF patients (0/5) or healthy control subjects (0/5). Comparison of antibody profiles between BALF and matching serum specimens revealed various recognition patterns favoring predominant production of anti-citrullinated HSP90 antibodies within the lung microenvironment-further supporting the connection between this antibody specificity and parenchymal lung disease. Equally important, qualitative as well as quantitative differences in anti-citrullinated HSP90 profiles between BALF and serum indicate that the lung plays a direct role in shaping the immune repertoire of RA/RA-ILD.
['Harlow|Lisa|L|', 'Gochuico|Bernadette R|BR|', 'Rosas|Ivan O|IO|', 'Doyle|Tracy J|TJ|', 'Osorio|Juan C|JC|', 'Travers|Timothy S|TS|', 'Camacho|Carlos C|CC|', 'Oddis|Chester V|CV|', 'Ascherman|Dana P|DP|']
D000906:Antibodies D000918:Antibody Specificity D001172:Arthritis, Rheumatoid D015415:Biomarkers D001992:Bronchoalveolar Lavage Fluid D018604:Epitope Mapping D005260:Female D018841:HSP90 Heat-Shock Proteins D006801:Humans D008168:Lung D008171:Lung Diseases D008297:Male
Bronchoalveolar lavage fluid HSP90 Interstitial lung disease Citrullination Autoantibodies Rheumatoid arthritis