Comprehensive characterization of circular RNAs in osteosarcoma cell lines.
Circular RNA (circRNA) is a looped noncoding RNA with a stable structure and tissue-specific expression and widely reported to regulate cancer initiation and progression. However, the circRNA expression patterns and their roles in osteosarcoma initiation and progression are still poorly understood. In this study, we characterized the landscape of circRNAs in osteosarcoma (OS) cell lines, and calculated the epithelial-mesenchymal transition (EMT) scores for OS cell lines. The differential expression analysis revealed that the EMT-related genes were significantly upregulated in the OS cell lines with higher metastatic potentials, and some inflammation-related pathways and pathways involved in cell-cell communications were enriched by these upregulated genes. Furthermore, we constructed a circRNA-based competing endogenous RNA (ceRNA) network, which consisted of 5 circRNAs, 17 miRNAs, and 73 mRNAs. Particularly, hsa_circ_0085360, which had the highest correlation with TRPS1, were characterized by some cancer-related pathways, and TRPS1 and its target gene FGFR3 were closely associated with both event-free survival and overall survival of OS, indicating that hsa_circ_0085360 might have the potential to predict the OS prognosis. In summary, we profiled the circRNA expression patterns in OS, predicted their functionality, and explored the underlying mechanism and prognostic values, which might provide some evidences for OS-related circRNA researches.
['Xiong|Wen|W|', 'Zhang|Yun|Y|', 'Yu|Huaixi|H|']
D045744:Cell Line, Tumor D058750:Epithelial-Mesenchymal Transition D015894:Genome, Human D006801:Humans D009362:Neoplasm Metastasis D012516:Osteosarcoma D011379:Prognosis D000079962:RNA, Circular D012334:RNA, Neoplasm
Circular RNA (circRNA) Epithelial-mesenchymal transition (EMT) Metastatic potential Competing endogenous RNA Underlying mechanism