Six species of nontuberculous mycobacteria carry non-identical 16S rRNA gene copies.
Nontuberculous mycobacteria (NTM) can carry two or more 16S rRNA gene copies that are, in some instances, non-identical. In this study, we used a combined cloning and sequencing approach to analyze 16S rRNA gene sequences of six NTM species, Mycobacterium cosmeticum, M. pallens, M. hodleri, M. crocinum, M. flavescens, and M. xenopi. Our approach facilitated the identification of two distinct gene copies in each species. The two M. cosmeticum genes had a single nucleotide difference, whereas two nucleotide polymorphisms were identified in M. hodleri, M. flavescens, and M. xenopi. M. pallens had a difference in four nucleotides and M. crocinum - in 23 nucleotides. Thus, we showed that the six NTM species possess at least two non-identical 16S rRNA gene copies. The full-length sequences of the intraspecies 16S rRNA variants will facilitate NTM identification and sequence analysis of specimens or other samples.
['Takeda|Keita|K|', 'Chikamatsu|Kinuyo|K|', 'Igarashi|Yuriko|Y|', 'Morishige|Yuta|Y|', 'Murase|Yoshiro|Y|', 'Aono|Akio|A|', 'Yamada|Hiroyuki|H|', 'Takaki|Akiko|A|', 'Mitarai|Satoshi|S|']
D015373:Bacterial Typing Techniques D003001:Cloning, Molecular D004269:DNA, Bacterial D018628:Gene Dosage D006801:Humans D009170:Nontuberculous Mycobacteria D020641:Polymorphism, Single Nucleotide D012336:RNA, Ribosomal, 16S D016415:Sequence Alignment D017422:Sequence Analysis, DNA
Nontuberculous mycobacteria 16S rRNA Gene copy Mycobacterium