Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell development.
BACKGROUND
Human oligodendrocyte precursor cells (hOPCs) are an important source of myelinating cells for cell transplantation to treat demyelinating diseases. Myelin oligodendrocytes develop from migratory and proliferative hOPCs. It is well known that NG2 and A2B5 are important biological markers of hOPCs. However, the functional differences between the cell populations represented by these two biomarkers have not been well studied in depth.


OBJECTIVE
To study the difference between NG2 and A2B5 cells in the development of human oligodendrocyte progenitor cells.


METHODS
Using cell sorting technology, we obtained NG2+/-, A2B5+/- cells. Further research was then conducted via in vitro cell proliferation and migration assays, single-cell sequencing, mRNA sequencing, and cell transplantation into shiverer mice.


RESULTS
The proportion of PDGFR-α + cells in the negative cell population was higher than that in the positive cell population. The migration ability of the NG2+/-, A2B5+/- cells was inversely proportional to their myelination ability. The migration, proliferation, and myelination capacities of the negative cell population were stronger than those of the positive cell population. The ability of cell migration and proliferation of the four groups of cells from high to low was: A2B5- > NG2- > NG2+ > A2B5+. The content of PDGFR-α+ cells and the ability of cell differentiation from high to low was: NG2- > A2B5- > A2B5+ > NG2+.


CONCLUSION
In summary, NG2+  and A2B5+ cells have poor myelination ability due to low levels of PDGFR-α+ cells. Therefore, hOPCs with a higher content of PDGFR-α+ cells may have a better effect in the cell transplantation treatment of demyelinating diseases.
['Zhou|Haipeng|H|', 'He|Ying|Y|', 'Wang|Zhaoyan|Z|', 'Wang|Qian|Q|', 'Hu|Caiyan|C|', 'Wang|Xiaohua|X|', 'Lu|Siliang|S|', 'Li|Ke|K|', 'Yang|Yinxiang|Y|', 'Luan|Zuo|Z|']
D000818:Animals D000941:Antigens D015415:Biomarkers D002454:Cell Differentiation D006801:Humans D051379:Mice D009186:Myelin Sheath D000073637:Oligodendrocyte Precursor Cells D009836:Oligodendroglia
Human oligodendrocyte progenitor cells NG2 A2B5 Biological markers Cell sorting