Follicular fluid exosomes: Important modulator in proliferation and steroid synthesis of porcine granulosa cells.
Granulosa cells (GCs) are regulated by various factors during ovarian development. However, there are few reports on the role of follicular fluid exosomes in ovarian GCs. In this study, porcine ovarian GCs were used to explore the effects of follicular fluid exosomes on GCs. GCs were treated with in vitro, and the changes in cell proliferation, steroid synthesis, and associated signal pathways were detected. The results showed that exosomes increased cell viability and altered the gene expression profile of GCs. Exosomes also increased the level of gene expression associated with both proliferation and progesterone synthesis, in which the MAPK/ERK and WNT/B-CATENIN pathways were involved. In addition, exosome-carried microRNAs were identified by high-throughput sequencing, and exosomal miR-31-5p was found to promote the proliferation of GCs and progesterone synthesis via the WNT/B-CATENIN pathway by targeting the SFRP4 follicle growth inhibitor. In conclusion, this study has demonstrated that exosomes are essential substances involved in regulating the physiological function of GCs.
['Yuan|Chenfeng|C|', 'Li|Zheng|Z|', 'Zhao|Yun|Y|', 'Wang|Xin|X|', 'Chen|Lu|L|', 'Zhao|Zijiao|Z|', 'Cao|Maosheng|M|', 'Chen|Tong|T|', 'Iqbal|Tariq|T|', 'Zhang|Boqi|B|', 'Fan|Wenjing|W|', 'Wei|Yameng|Y|', 'Li|Chunjin|C|', 'Zhou|Xu|X|']
D000818:Animals D017209:Apoptosis D049109:Cell Proliferation D055354:Exosomes D005260:Female D015571:Follicular Fluid D020869:Gene Expression Profiling D005786:Gene Expression Regulation D006107:Granulosa Cells D035683:MicroRNAs D006080:Ovarian Follicle D013256:Steroids D013552:Swine
exosome proliferation steroid granulosa cell