Realization of 19F MRI oximetry method using perfluorodecalin.
OBJECTIVE
To identify the technical aspects of the potential use of clinically approved perfluorodecalin (PFD, C10F18) for 19F magnetic resonance imaging (MRI) oximetry method at high magnetic field 7.05 T.


MATERIALS AND METHODS
19F T1 measurements were made on a set of PFD samples with different oxygen contents (0%, 21%, and 100%) at room (21 °C) and body temperature (37 °C). In vivo MRI studies were carried out on one healthy rat and two rats with C6 brain glioma.


RESULTS
The selective excitation of the magnetically equivalent 19F nuclei of CF2 groups of trans-isomer of PFD, which give a doublet at a frequency of about - 140 ppm (in relation the chemical shift of trifluoroacetic acid, which is - 76.55 ppm) should be done for correct implementation of 19F MRI oximetry method. The amount of PFD equal to 30 μl is the optimal for obtaining reliable data on the measured T1 values. In this case, the standard deviation of T1 does not exceed 5%. In vivo MRI studies showed that the values of the partial pressure of oxygen (pO2) decrease from normal values of about 38 mmHg (healthy brain) to almost 0 mmHg at the last stage of tumor growth.


CONCLUSION
The study showed the feasibility of the successful application of PFD for 19F MRI oximetry method.
['Gulyaev|Mikhail V|MV|', 'Kuznetsova|Aleksandra V|AV|', 'Silachev|Denis N|DN|', 'Danilina|Tatyana I|TI|', 'Gervits|Lev L|LL|', 'Pirogov|Yury A|YA|']
D000818:Animals D001932:Brain Neoplasms D045744:Cell Line, Tumor D005461:Fluorine D005466:Fluorocarbons D005910:Glioma D007554:Isotopes D060526:Magnetic Fields D008279:Magnetic Resonance Imaging D008297:Male D010092:Oximetry D010100:Oxygen D019047:Phantoms, Imaging D051381:Rats D017208:Rats, Wistar
19F MRI oximetry method Perfluorodecalin C6 rat brain glioma RARE-VTR