Development of a method to screen and isolate potential α-glucosidase inhibitors from Panax japonicus C.A. Meyer by ultrafiltration, liquid chromatography, and counter-current chromatography.
A new assay based on ultrafiltration, liquid chromatography and mass spectrometry was developed for the rapid screening and identification of the ligands for α-glucosidase from the extract of Panax japonicus. Six saponins were identified as α-glucosidase inhibitors. Subsequently, the specific binding ligands, namely, notoginsenoside R1 , ginsenoside Rb1 , chikusetsusaponin V, chikusetsusaponin IV, chikusetsusaponin IVa, and ginsenoside Rd (the purities were 94.18, 95.43, 96.09, 93.26, 94.50, 93.86%, respectively) were separated by counter-current chromatography using two-phase solvent systems composed of tert-butyl methyl ether, acetonitrile, 0.1% aqueous formic acid (3.8:1.0:4.4, v/v/v) and the solvent system composed of methylene chloride, isopropanol, methanol, 0.1% aqueous formic acid (5.8:1.0:6.0:2.2, v/v/v). The results demonstrate that ultrafiltration, liquid chromatography and mass spectrometry combined with high-speed counter-current chromatography might provide not only a powerful tool for screening and isolating α-glucosidase inhibitors in complex samples but also a useful platform for discovering bioactive compounds for the prevention and treatment of diabetes mellitus.
['Li|Sainan|S|', 'Tang|Ying|Y|', 'Liu|Chunming|C|', 'Zhang|Yuchi|Y|']
D000097:Acetonitriles D002853:Chromatography, Liquid D003920:Diabetes Mellitus D004791:Enzyme Inhibitors D005561:Formates D036145:Ginsenosides D065089:Glycoside Hydrolase Inhibitors D006801:Humans D008024:Ligands D013058:Mass Spectrometry D008738:Methyl Ethers D008962:Models, Theoretical D015999:Multivariate Analysis D009828:Oleanolic Acid D005894:Panax D010936:Plant Extracts D012503:Saponins D012997:Solvents D021241:Spectrometry, Mass, Electrospray Ionization D014462:Ultrafiltration D000520:alpha-Glucosidases
α-Glucosidase inhibitors Panax japonicus C.A. Meyer Saponins High-speed counter-current chromatography