Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement.
The latest data link the chronic consumption of large amounts of fructose present in food with the generation of hypertension and disturbances in carbohydrate and lipid metabolism, which promote the development of obesity, non-alcoholic fatty liver disease, insulin resistance, and type 2 diabetes. This effect is possible after fructose is absorbed by the small intestine cells and, to a lesser extent, by hepatocytes. Fructose transport is dependent on proteins from the family of glucose transporters (GLUTs), among which GLUT5 selectively absorbs fructose from the intestine. In this study, we examined the effect of four phenolic-rich extracts obtained from A. graveolens, B. juncea, and M. chamomilla on fructose uptake by Caco-2 cells. Extracts from B. juncea and M. chamomilla most effectively reduced fluorescent fructose analogue (NBDF) accumulation in Caco-2, as well as downregulated GLUT5 protein levels. These preparations were able to decrease the mRNA level of genes encoding transcription factors regulating GLUT5 expression-thioredoxin-interacting protein (TXNIP) and carbohydrate-responsive element-binding protein (ChREBP). Active extracts contained large amounts of apigenin and flavonols. The molecular docking simulation suggested that some of identified phenolic constituents can play an important role in the inhibition of GLUT5-mediated fructose transport.
['Zakłos-Szyda|Małgorzata|M|0000-0001-8341-1654', 'Pietrzyk|Nina|N|0000-0002-3279-0612', 'Kowalska-Baron|Agnieszka|A|', 'Nowak|Adriana|A|0000-0002-1865-4833', 'Chałaśkiewicz|Katarzyna|K|0000-0003-3321-397X', 'Ratajewski|Marcin|M|0000-0001-5391-5843', 'Budryn|Grażyna|G|0000-0002-8050-3702', 'Koziołkiewicz|Maria|M|']
D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors D001692:Biological Transport D018938:Caco-2 Cells D002352:Carrier Proteins D004032:Diet D005632:Fructose D005786:Gene Expression Regulation D051296:Glucose Transporter Type 5 D006801:Humans D010636:Phenols D010936:Plant Extracts D012333:RNA, Messenger
fructose Caco-2 GLUT5 phenolic compounds