Monosynaptic tracing maps brain-wide afferent oligodendrocyte precursor cell connectivity.
Neurons form bona fide synapses with oligodendrocyte precursor cells (OPCs), but the circuit context of these neuron to OPC synapses remains incompletely understood. Using monosynaptically-restricted rabies virus tracing of OPC afferents, we identified extensive afferent synaptic inputs to OPCs residing in secondary motor cortex, corpus callosum, and primary somatosensory cortex of adult mice. These inputs primarily arise from functionally-interconnecting cortical areas and thalamic nuclei, illustrating that OPCs have strikingly comprehensive synaptic access to brain-wide projection networks. Quantification of these inputs revealed excitatory and inhibitory components that are consistent in number across brain regions and stable in barrel cortex despite whisker trimming-induced sensory deprivation.
['Mount|Christopher W|CW|0000-0003-1635-2492', 'Yalçın|Belgin|B|0000-0001-9689-9062', 'Cunliffe-Koehler|Kennedy|K|', 'Sundaresh|Shree|S|', 'Monje|Michelle|M|0000-0002-3547-237X']
D000344:Afferent Pathways D000818:Animals D003337:Corpus Callosum D051379:Mice D009044:Motor Cortex D056347:Neuroanatomical Tract-Tracing Techniques D009474:Neurons D000073637:Oligodendrocyte Precursor Cells D013003:Somatosensory Cortex
oligodendrocyte precursor cell axon-glial synapse monosynaptic viral tracing mouse myelin neuron-glia interactions neuroscience