Application of high-speed counter-current chromatography and HPLC to separate and purify of three polyacetylenes from Platycodon grandiflorum.
Three polyacetylenes were isolated and purified from Platycodon grandiflorum A. DC for the first time by high-speed counter-current chromatography using a two-phase solvent system composed of hexane/ethyl acetate/methanol/water (1:31:1:31, v/v/v/v) and high-performance liquid chromatography with an Agilent ZORBAX® SB-C18 column (4.6 mm × 150 mm, 5 μm). After separation by high-speed counter-current chromatography and high-performance liquid chromatography, we obtained 3.5 mg of platetyolin A, 4.1 mg of platetyolin B, and 18.1 mg of lobetyolin with purities of 97.2, 96.7, and 96.9%, respectively. The purity of each compound was assessed by high-performance liquid chromatography and the chemical structures were evaluated by high-resolution electrospray ionization time-of-flight mass spectrometry and one- and two-dimensional NMR spectroscopy. Among the isolated compounds, platetyolin A and platetyolin B are newly reported compounds.
['Chen|Bao|B|', 'Liu|Zhengbo|Z|', 'Zhang|Yuwei|Y|', 'Li|Wei|W|', 'Sun|Yanjun|Y|', 'Wang|Yufang|Y|', 'Wang|Yanhua|Y|', 'Sun|Yinshi|Y|http://orcid.org/0000-0001-6865-3081']
D000085:Acetates D002845:Chromatography D002851:Chromatography, High Pressure Liquid D003377:Countercurrent Distribution D006586:Hexanes D009682:Magnetic Resonance Spectroscopy D000432:Methanol D031238:Platycodon D053279:Polyynes D016062:Porosity D012997:Solvents D021241:Spectrometry, Mass, Electrospray Ionization
high-speed counter-current chromatography polyacetylenes platetyolin lobetyolin traditional Chinese medicine