Identification of key genes and molecular mechanisms associated with temperature stress in lentil.
Extreme temperature is one of the serious threats to crop production in present and future scenarios of global climate changes. Lentil (Lens culinaris) is an important crop, and there is a serious lack of genetic information regarding environmental and temperature stresses responses. This study is the first report of evaluation of key genes and molecular mechanisms related to temperature stresses in lentil using the RNA sequencing technique. De novo transcriptome assembly created 44,673 contigs and differential gene expression analysis revealed 7494 differentially expressed genes between the temperature stresses and control group. Basic annotation of generated transcriptome assembly in our study led to the identification of 2765 novel transcripts that have not been identified yet in lentil genome draft v1.2. In addition, several unigenes involved in mechanisms of temperature sensing, calcium and hormone signaling and DNA-binding transcription factor activity were identified. Also, common mechanisms in response to temperature stresses, including the proline biosynthesis, the photosynthetic light reactions balancing, chaperone activity and circadian rhythms, are determined by the hub genes through the protein-protein interaction networks analysis. Deciphering the mechanisms of extreme temperature tolerance would be a new way for developing crops with enhanced plasticity against climate change. In general, this study has identified set of mechanisms and various genes related to cold and heat stresses which will be useful in better understanding of the lentil's reaction to temperature stresses.
['Sohrabi|Seyed Sajad|SS|', 'Ismaili|Ahmad|A|', 'Nazarian-Firouzabadi|Farhad|F|', 'Fallahi|Hossein|H|', 'Hosseini|Seyedeh Zahra|SZ|']
D057231:Climate Change D003080:Cold Temperature D058639:Cold-Shock Response D018556:Crops, Agricultural D020869:Gene Expression Profiling D018506:Gene Expression Regulation, Plant D018869:Heat-Shock Response D006358:Hot Temperature D027806:Lens Plant D058977:Molecular Sequence Annotation D010788:Photosynthesis D060066:Protein Interaction Maps D013312:Stress, Physiological D013696:Temperature D059467:Transcriptome
Lentil Climate change De novo transcriptome assembly Networks analysis Cold stress