Effects of estrogen deficiency followed by streptozotocin-induced diabetes on periodontal tissues of female rats.
Although both estrogen deficiency and diabetes contribute to periodontal tissue deterioration, the combined effects of these conditions on periodontium is unknown. Thus, we analyzed the combined effects of ovariectomy followed by streptozotocin (STZ)-induced diabetes on periodontal tissues of rats. Twenty adult rats were ovariectomized (OVX) or SHAM-operated (SHAM). After 3 weeks, the rats received an intraperitoneal injection of STZ (60 mg/kg/body weight) to induce diabetes or vehicle (blank) solution. The groups were assigned as follows (n = 5): SHAM-vehicle (SHAM), OVX-vehicle (OVX), SHAM + STZ (SHAM-Di), and OVX + STZ (OVX-Di). Seven weeks post-diabetes induction, the rats were euthanized. Blood samples were collected for glucose measurements and maxillae were processed for paraffin embedding. Sections stained with hematoxylin/eosin, Masson's trichrome, and picrosirius-red were used for alveolar bone loss and collagen fiber analysis in the lamina propria. Immunohistochemistry was performed for runt-related transcription factor 2 (Runx2), matrix metalloproteinase 9 (MMP-9), and tryptase detection. Alveolar bone loss and fewer collagen fibers were observed in the OVX-Di group, collagen fibers with irregular organization, and MMP-9 immunoreactivity were more evident in diabetic groups, and MMP-9-positive osteoclasts on alveolar bone surface were noticed in all groups. The OVX-Di group showed lower Runx2 immunoreactivity (osteoblast formation marker), and more tryptase-positive cells (mast cell marker) in the alveolar bone marrow. Our results indicate that estrogen depletion, followed by STZ-induced diabetes, promotes periodontal tissue deterioration that is more evident than both interventions applied alone. Furthermore, our results points to a possible participation of bone-derived mast cells in this process.
['Sasso|Gisela Rodrigues da Silva|GRDS|http://orcid.org/0000-0002-6583-1329', 'Florencio-Silva|Rinaldo|R|', 'da Fonseca|Caio Cesar Navarrete|CCN|', 'Cezar|Luana Carvalho|LC|', 'Carbonel|Adriana Aparecida Ferraz|AAF|', 'Gil|Cristiane Damas|CD|', 'Simões|Manuel de Jesus|MJ|', 'Girão|Manoel João Batista Castello|MJBC|']
D016301:Alveolar Bone Loss D000818:Animals D015519:Bone Density D003921:Diabetes Mellitus, Experimental D004967:Estrogens D005260:Female D008407:Mast Cells D010010:Osteoclasts D010011:Osteocytes D010052:Ovariectomy D010513:Periodontal Ligament D010519:Periodontium D051381:Rats D017208:Rats, Wistar D013311:Streptozocin
Estrogen deficiency Diabetes Rats Periodontium