Targeting of the Dosage-Compensated Male X-Chromosome during Early Drosophila Development.
Dosage compensation, which corrects for the imbalance in X-linked gene expression between XX females and XY males, represents a model for how genes are targeted for coordinated regulation. However, the mechanism by which dosage compensation complexes identify the X chromosome during early development remains unknown because of the difficulty of sexing embryos before zygotic transcription using X- or Y-linked reporter transgenes. We used meiotic drive to sex Drosophila embryos before zygotic transcription and ChIP-seq to measure the dynamics of dosage compensation factor targeting. The Drosophila male-specific lethal dosage compensation complex (MSLc) requires the ubiquitous zinc-finger protein chromatin-linked adaptor for MSL proteins (CLAMP) to identify the X chromosome. We observe a multi-stage process in which MSLc first identifies CLAMP binding sites throughout the genome, followed by concentration at the strongest X-linked MSLc sites. We provide insight into the dynamics of binding site recognition by a large transcription complex during early development.
['Rieder|Leila Elizabeth|LE|', 'Jordan|William Thomas|WT|', 'Larschan|Erica Nicole|EN|']
D000818:Animals D002843:Chromatin D004303:Dosage Compensation, Genetic D029721:Drosophila Proteins D004331:Drosophila melanogaster D004625:Embryo, Nonmammalian D047108:Embryonic Development D005260:Female D018507:Gene Expression Regulation, Developmental D008297:Male D008540:Meiosis D015203:Reproducibility of Results D014960:X Chromosome
Drosophila dosage compensation meiotic drive CLAMP MSL complex Segregation distorter chromatin domain transcription factor zygotic genome activation