Influence of the RPL34 gene on the growth and metastasis of oral squamous cell carcinoma cells.
BACKGROUND
Oral squamous cell carcinoma (OSCC) accounts for almost 3% of all malignant tumors all over the world. This study aims to investigate the correlation of RPL34 with the cell growth and metastasis of oral squamous cell carcinoma (OSCC) as well as its clinical prognosis.


METHOD
Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry were conducted to determine the RPL34 expression in 85 OSCC tissues and 30 normal oral mucosa tissues. Besides, OSCC cell lines SCC-4 were divided into blank group, negative control (NC) group and RPL34-shRNA group. The qRT-PCR and western blot were performed to measure RPL34 expression, CCK-8 and flow cytometry to observe cell growth and apoptosis, and wound healing and transwell to detect cell migration and invasion.


RESULTS
The RPL34 gene expression was up-regulated in OSCC tissues and cells. The RPL34 expression was significantly correlated with differentiation degree, TNM stage and lymph node metastasis. Patients with positive RPL34 expression had a poorer prognosis. After inhibition of RPL34 expression, the proliferation of SCC-4 cells was slowed down at 24h, 48h, 72h and 96h respectively, and both the migration distance and the number of invasive cells were reduced, while there was an increase in the ratio of cells at G0/G1 stage and cell apoptosis.


CONCLUSION
The RPL34 gene was highly expressed in OSCC, while silencing RPL34 could block cell proliferation and metastasis, but promote cell apoptosis, suggesting the RPL34 gene to be a new promising clinical target for OSCC therapy.
['Dai|Jing|J|', 'Wei|Wei|W|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D017209:Apoptosis D015153:Blotting, Western D002294:Carcinoma, Squamous Cell D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D005260:Female D005434:Flow Cytometry D015972:Gene Expression Regulation, Neoplastic D020868:Gene Silencing D006801:Humans D007150:Immunohistochemistry D008207:Lymphatic Metastasis D008297:Male D008875:Middle Aged D009062:Mouth Neoplasms D009361:Neoplasm Invasiveness D011379:Prognosis D060888:Real-Time Polymerase Chain Reaction D012269:Ribosomal Proteins
RPL34 Metastasis Oral squamous cell carcinoma Prognosis Apoptosis Invasion