Identification of a phage-derived depolymerase specific for KL64 capsule of Klebsiella pneumoniae and its anti-biofilm effect.
The increasing prevalence of Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a serious threat to global health. Phages and phage-derived enzymes gained increasing attention for controling CRKP infections. In this study, a lytic phage P510 infecting KL64 type K. pneumoniae was isolated and characterized. Whole genome analysis and electron microscopy analysis showed that phage P510 belonged to genus Przondovirus, family Autographiviridae, the order Caudovirales. The tail fiber protein of the phage was predicted to encode capsule depolymerase. Further analysis demonstrated that recombinant depolymerase P510dep had polysaccharide-degrading activity against KL64-types capsule of K. pneumoniae, and its lysis spectrum matched to host range of phage P510. We also demonstrated that the recombinant depolymerase was able to significantly inhibit biofilm formation. The discovery of the phage-derived depolymerase lays the foundation for controlling the spread of CRKPs.
['Li|Min|M|', 'Li|Pei|P|', 'Chen|Long|L|', 'Guo|Genglin|G|', 'Xiao|Yuyi|Y|', 'Chen|Liang|L|', 'Du|Hong|H|', 'Zhang|Wei|W|http://orcid.org/0000-0002-3575-6225']
D001435:Bacteriophages D018441:Biofilms D000073182:Carbapenem-Resistant Enterobacteriaceae D017899:Caudovirales D016679:Genome, Viral D006026:Glycoside Hydrolases D006801:Humans D007711:Klebsiella pneumoniae D014764:Viral Proteins
Depolymerase Klebsiella pneumoniae Carbapenem-resistant Biofilm Bacteriophages Capsular type