Rapid Gene Isolation Using MutChromSeq.
MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.
['Steuernagel|Burkhard|B|', 'Vrána|Jan|J|', 'Karafiátová|Miroslava|M|', 'Wulff|Brande B H|BBH|', 'Doležel|Jaroslav|J|']
D032461:Chromosomes, Plant D018744:DNA, Plant D005434:Flow Cytometry D018506:Gene Expression Regulation, Plant D018745:Genome, Plant D023281:Genomics D017404:In Situ Hybridization, Fluorescence D009154:Mutation D010944:Plants D011123:Polyploidy D017422:Sequence Analysis, DNA
Chromosome isolation FISHIS Flow cytometry and chromosome sorting Gene cloning Mutational genomics