Type VI secretion system is not required for virulence on rice but for inter-bacterial competition in Xanthomonas oryzae pv. oryzicola.
The type VI secretion system (T6SS), a multifunctional protein secretion device, plays very important roles in bacterial killing and/or virulence to eukaryotic cells. Although T6SS genes have been found in many Xanthomonas species, the biological function of T6SSs has not been elucidated in most xanthomonads. In this study, we identified two phylogenetically distinct T6SS clusters, T6SS1 and T6SS2, in a newly sequenced Chinese strain GX01 of Xanthomonas oryzea pv. oryzicola (Xoc) which causes bacterial leaf streak (BLS) of rice (Oryza sativa L.). Mutational assays demonstrated that T6SS1 and T6SS2 are not required for the virulence of Xoc GX01 on rice. Nevertheless, we found that T6SS2, but not T6SS1, played an important role in bacterial killing. Transcription and secretion analysis revealed that hcp2 gene is actively expressed and that Hcp2 protein is secreted via T6SS. Moreover, several candidate T6SS effectors were predicted by bioinformatics analysis that might play a role in the antibacterial activity of Xoc. This is the first report to investigate the type VI secretion system in Xanthomonas oryzae. We speculate that Xoc T6SS2 might play an important role in inter-bacterial competition, allowing this plant pathogen to gain niche advantage by killing other bacteria.
['Zhu|Ping-Chuan|PC|', 'Li|Yi-Ming|YM|', 'Yang|Xia|X|', 'Zou|Hai-Fan|HF|', 'Zhu|Xiao-Lin|XL|', 'Niu|Xiang-Na|XN|', 'Xu|Ling-Hui|LH|', 'Jiang|Wei|W|', 'Huang|Sheng|S|', 'Tang|Ji-Liang|JL|', 'He|Yong-Qiang|YQ|']
D019295:Computational Biology D015964:Gene Expression Regulation, Bacterial D016680:Genome, Bacterial D056265:Microbial Interactions D009154:Mutation D012275:Oryza D010641:Phenotype D010802:Phylogeny D010935:Plant Diseases D000069376:Type VI Secretion Systems D014774:Virulence D014974:Xanthomonas
Type VI secretion system (T6SS) Xanthomonas oryzea pv. oryzicola T6SS effectors Bacterial competition Hcp secretion