Annexin A3 is a mammary marker and a potential neoplastic breast cell therapeutic target.
Breast cancers are the most common cancer-affecting women; critically the identification of novel biomarkers for improving early detection, stratification and differentiation from benign tumours is important for the reduction of morbidity and mortality.To identify and functionally characterise potential biomarkers, we used mass spectrometry (MS) to analyse serum samples representing control, benign breast disease (BBD) and invasive breast cancer (IDC) patients. Complementary and multidimensional proteomic approaches were used to identify and validate novel serum markers.Annexin A3 (ANX A3) was found to be differentially expressed amongst different breast pathologies. The diagnostic value of serum ANX A3 was subsequently validated by ELISA in an independent serum set representing the three groups. Here, ANX A3 was significantly upregulated in the benign disease group sera compared with other groups (P < 0.0005).In addition, paired breast tissue immunostaining confirmed that ANX A3 was abundantly expressed in benign and to a lesser extent malignant neoplastic epithelium. Finally, we illustrated ANX A3 expression in cell culture lysates and conditioned media from neoplastic breast cell lines, and its role in neoplastic breast cell migration in vitro.This study confirms the novel role of ANX A3 as a mammary biomarker, regulator and therapeutic target.
['Zeidan|Bashar|B|', 'Jackson|Thomas R|TR|', 'Larkin|Samantha E T|SE|', 'Cutress|Ramsey I|RI|', 'Coulton|Gary R|GR|', 'Ashton-Key|Margaret|M|', 'Murray|Nick|N|', 'Packham|Graham|G|', 'Gorgoulis|Vassilis|V|', 'Garbis|Spiros D|SD|', 'Townsend|Paul A|PA|']
D000368:Aged D017318:Annexin A3 D014408:Biomarkers, Tumor D001940:Breast D001943:Breast Neoplasms D045744:Cell Line, Tumor D002465:Cell Movement D017077:Culture Media, Conditioned D004797:Enzyme-Linked Immunosorbent Assay D004848:Epithelium D005260:Female D015972:Gene Expression Regulation, Neoplastic D020868:Gene Silencing D006801:Humans D007150:Immunohistochemistry D061986:MCF-7 Cells D013058:Mass Spectrometry D008875:Middle Aged D009361:Neoplasm Invasiveness D009362:Neoplasm Metastasis D040901:Proteomics D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Annexin A3 breast cancer biomarker migration neoplasm