RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana.
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between gaseous carbon dioxide (CO2 ) and ribulose-1,5-bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBisCO is still emerging. In bacteria and in the C4 plant Zea mays, a protein with distant homology to pterin-4α-carbinolamine dehydratase (PCD) has recently been shown to be involved in RuBisCO assembly. However, studies of the homologous PCD-like protein (RAF2, RuBisCO assembly factor 2) in the C3 plant Arabidopsis thaliana (A. thaliana) have so far focused on its role in hormone and stress signaling. We investigated whether A. thalianaRAF2 is also involved in RuBisCO assembly. We localized RAF2 to the soluble chloroplast stroma and demonstrated that raf2 A. thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBisCO. We concluded that the RAF2 protein is probably involved in RuBisCO assembly in the C3 plant A. thaliana.
['Fristedt|Rikard|R|', 'Hu|Chen|C|', 'Wheatley|Nicole|N|', 'Roy|Laura M|LM|', 'Wachter|Rebekka M|RM|', 'Savage|Linda|L|', 'Harbinson|Jeremy|J|', 'Kramer|David M|DM|', 'Merchant|Sabeeha S|SS|', 'Yeates|Todd|T|', 'Croce|Roberta|R|']
D000483:Alleles D017360:Arabidopsis D029681:Arabidopsis Proteins D002736:Chloroplasts D055786:Gene Knockout Techniques D010802:Phylogeny D012273:Ribulose-Bisphosphate Carboxylase D016415:Sequence Alignment D020524:Thylakoids
RAF2 RuBisCO RuBisCO assembly factor chloroplast Atg5 g51110 RuBisCO aggregation SDIRIP1 abscisic acid