Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform.
In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored onto SH-DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs were successfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using environmentally friendly procedures.
['Barroso|M Fátima|MF|', 'Luna|M Alejandra|MA|', 'Tabares|Juan S Flores|JS|', 'Delerue-Matos|Cristina|C|', 'Correa|N Mariano|NM|', 'Moyano|Fernando|F|', 'Molina|Patricia G|PG|']
gold nanoparticles covalently decorated vesicles immunosensors design synthesis