Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain.
BACKGROUND
Brucellosis is a worldwide zoonotic infectious disease that is transmitted in various ways and causes great harm to humans and animals. The brucellosis pathogen is Brucella, which mainly resides in macrophage cells and survives and replicates in host cells. However, the mechanisms underlying Brucella survival in macrophage cells have not been thoroughly elucidated to date. Peroxiredoxin 6 (Prdx6) is a bifunctional protein that shows not only GSH peroxidase activity but also phospholipase A2 activity and plays important roles in combating oxidative damage and regulating apoptosis.


RESULTS
Recombinant mouse (Mus musculus) Prdx6 (MmPrdx6) was expressed and purified, and monoclonal antibodies against MmPrdx6 were prepared. Using the Brucella suis S2 strain to infect RAW264.7 murine macrophages, the level of intracellular Prdx6 expression first decreased and later increased following infection. Overexpressing Prdx6 in macrophages resulted in an increase in B. suis S2 strain levels in RAW264.7 cells, while knocking down Prdx6 reduced the S2 levels in cells.


CONCLUSIONS
Host Prdx6 can increase the intracellular survival of B. suis S2 strain and plays a role in Brucella infection.
['Wang|Lu-Lu|LL|', 'Chen|Xiao-Feng|XF|', 'Hu|Pan|P|', 'Lu|Shi-Ying|SY|', 'Fu|Bao-Quan|BQ|', 'Li|Yan-Song|YS|', 'Zhai|Fei-Fei|FF|', 'Ju|Dan-Di|DD|', 'Zhang|Shi-Jun|SJ|', 'Shui|Yi-Ming|YM|', 'Chang|Jiang|J|', 'Ma|Xiao-Long|XL|', 'Su|Bing|B|', 'Zhou|Yu|Y|', 'Liu|Zeng-Shan|ZS|', 'Ren|Hong-Lin|HL|http://orcid.org/0000-0002-3364-8335']
D000818:Animals D041021:Brucella suis D002006:Brucellosis D005260:Female D005786:Gene Expression Regulation D055785:Gene Knockdown Techniques D008264:Macrophages D051379:Mice D008807:Mice, Inbred BALB C D054465:Peroxiredoxin VI D000067996:RAW 264.7 Cells
Host Prdx6 Intracellular survival Brucella Macrophage