Effect of mixing ratio and total solids content on temperature-phased anaerobic codigestion of rice straw and pig manure: Biohythane production and microbial structure.
Long-term semi-continuous experiments were carried out under three feedstock conditions to study the effects of mixing ratio and total solids (TS) content on temperature-phased anaerobic codigestion of rice straw (RS) and pig manure (PM). The results showed that biohythane only produced from the mixture with 6% TS content and its average content were 12.83 ± 1.19% (hydrogen) and 23.68 ± 1.12% (methane). Increasing mixture TS content and decreasing its RS ratio increased biohythane production and organic matter removal by creating a suitable process pH and increasing the anaerobic reaction rates. The highest biohythane production of the mixture reached 73.09 ± 3.03 ml/g VS (hydrogen) and 235.81 ± 9.30 ml/g VS (methane) at a mixing ratio of 5:1 and TS content of 6%. A variety of hydrogen-producing bacteria were found in the thermophilic reactor and Clostridium_sensu_stricto_1 played an important role. Butyric acid fermentation is the main hydrogen-producing pathway. Methanobacterium and Methanosaeta were dominant archaea in the mesophilic reactor.
['Liu|Rongzhan|R|', 'Chen|Xiangyu|X|', 'Zhang|Ke|K|', 'Han|Yunping|Y|', 'Tong|Yeqi|Y|', 'Wang|Juan|J|', 'Xiao|Benyi|B|', 'Liu|Junxin|J|']
Mixing ratio Total solids content Rice straw Pig manure Biohythane production Temperature phased anaerobic codigestion