Pivotal role of the gut microbiota in congenital insensitivity to pain with anhidrosis.
BACKGROUND
Increasing evidence has shown that the occurrence and development of various human diseases are closely related to the gut microbiota. We compared the gut microbial communities of human subjects with congenital insensitivity to pain with anhidrosis (CIPA) and healthy controls (HCs) to assess whether fecal microbiota transplantation (FMT) into germ-free mice and mice in acute pain influenced the behaviors of the host.


METHODS
We utilized 16 s rRNA analysis to compare the gut microbial communities of CIPA subjects and HCs and assessed whether FMT into germ-free mice and mice in acute pain influenced the behaviors of the host.


RESULTS
In a 16 s RNA analysis, the CIPA group had significant decreases in the relative abundance of 11 bacteria, whereas 7 bacteria were significantly increased. In further animal experiments, the transplantation of fecal samples from CIPA patients to healthy mice significantly increased their scores on both the mechanical withdrawal test and the tail flick test; in an acute plantar incision model, scores were also significantly increased on the mechanical withdrawal test at 4 and 5 days after the operation. Moreover, pseudo-germ-free mice receiving fecal bacteria from patients with CIPA took significantly longer to escape and had a significantly longer path length on training days 1, 2, and 5 and also had fewer platform crossings and spent less time in the target quadrant in the probe trial.


CONCLUSIONS
Our results suggest that the gut microbiota in CIPA subjects plays a key role in behaviors. Therapeutic strategies for improving the gut microbiota might alleviate CIPA symptoms.
['Zhang|Mi|M|', 'Hong|Yishun|Y|', 'Wu|Wenyao|W|', 'Li|Ningbo|N|', 'Liu|Baowen|B|', 'Sun|Jiaoli|J|', 'Cao|Xueqin|X|', 'Ye|Ting|T|', 'Zhou|Ling|L|', 'Liu|Cunming|C|', 'Yang|Chun|C|http://orcid.org/0000-0003-1540-4385', 'Zhang|Xianwei|X|']
D000818:Animals D000069467:Fecal Microbiota Transplantation D005243:Feces D000069196:Gastrointestinal Microbiome D009477:Hereditary Sensory and Autonomic Neuropathies D006801:Humans D051379:Mice
Gut microbiota Congenital insensitivity to pain with anhidrosis Fecal microbiota transplantation Mental retardation Pain threshold