Comparative evaluation of loop-mediated isothermal amplification (LAMP) vs qPCR for detection of Toxoplasma gondii oocysts DNA in mussels.
The apicomplexan parasite Toxoplasma gondii can infect humans and cause toxoplasmosis. T. gondii has been highly prioritized among the foodborne parasites regarding its global impact on public health. Human infection can occur through multiple routes, including the ingestion of raw or undercooked food contaminated with T. gondii oocysts, such as fresh produce and bivalves. As filter-feeders, bivalves can accumulate and concentrate contaminants, including protozoan (oo)cysts. Although detection of T. gondii in different bivalves by molecular techniques (PCR and qPCR) has been achieved, routine application is currently limited by lack of sensitivity or equipment costs. Here, we describe the assessment of a loop-mediated isothermal amplification (LAMP)-based assay to detect T. gondii oocysts in spiked mussels. Detection limit was down to 5 oocysts/g in tissue and 5 oocyst/ml in hemolymph, and, under the experimental conditions tested, LAMP was found to provide a promising alternative to qPCR.
['Durand|Loïc|L|', 'La Carbona|Stéphanie|S|', 'Geffard|Alain|A|', 'Possenti|Alessia|A|', 'Dubey|Jitender P|JP|', 'Lalle|Marco|M|']
D000818:Animals D049872:Bivalvia D016054:DNA, Protozoan D004587:Electrophoresis, Agar Gel D005517:Foodborne Diseases D006458:Hemolymph D021141:Nucleic Acid Amplification Techniques D060888:Real-Time Polymerase Chain Reaction D012680:Sensitivity and Specificity D014122:Toxoplasma D014123:Toxoplasmosis
qPCR Toxoplasma gondii Mussels Molecular detection LAMP