PDL-1/PDL-2 blockade in mice dendritic cells by RNAi techniques to induce antitumor immunity.
AIM
We provided potent dendritic cells (DCs) for induction of stronger antitumor immune responses.


MATERIALS & METHODS
Using siRNA and shRNA systems, PDL-1 and PDL-2 were knocked down and then DC in vitro and in vivo properties were evaluated.


RESULTS
Mild suppression of PDL-1/PDL-2 molecules was accompanied by appropriate expression of DCs co-stimulatory molecules and release of proinflammatory cytokines. In vitro T-cell engagement induced the proliferation and secretion of Th1 cytokines. Injection of DCs to a 4T1 mice model induced intratumor CD8(+) infiltrating lymphocytes, splenocytes expansion, Th1 cytokine profile shift, and a mild drift to tumor growth inhibition and mice survival.


CONCLUSION
Manipulated DCs induced significant antitumor immunity, but this subject needs further evaluation in different animals.
['Daneshmandi|Saeed|S|', 'Pourfathollah|Ali Akbar|AA|', 'Karimi|Mohammad Hossein|MH|', 'Emadi-Baygi|Modjtaba|M|']
D000818:Animals D060890:B7-H1 Antigen D018414:CD8-Positive T-Lymphocytes D002277:Carcinoma D045744:Cell Line, Tumor D002465:Cell Movement D003713:Dendritic Cells D005260:Female D015316:Genetic Therapy D057809:HEK293 Cells D006801:Humans D016219:Immunotherapy, Adoptive D008213:Lymphocyte Activation D051379:Mice D008807:Mice, Inbred BALB C D008810:Mice, Inbred C57BL D009374:Neoplasms, Experimental D061027:Programmed Cell Death 1 Ligand 2 Protein D034741:RNA, Small Interfering D018417:Th1 Cells D047368:Tumor Burden D019139:Tumor Escape
PDL-1 PDL-2 dendritic cell siRNA shRNA