The effect of single-nucleotide polymorphism in the promoter region of bovine alpha-lactalbumin (LALBA) gene on LALBA expression in milk cells and milk traits of cows.
Polymorphisms of milk protein genes have been proposed as candidate markers for dairy production traits in cattle. In the present study, a polymorphism was detected in the 5'-flanking (promoter) region of the bovine alpha-lactalbumin (LALBA) gene, a T/C transition located at nucleotide -1,001 relative to the transcription start site g.-1001T > C (NC_037332.1:g.31183170T > C), which is recognizable with PstI restriction endonuclease. In silico analyses showed that this mutation created novel retinoid X receptor alpha and vitamin D receptor transcription factor binding sites. Real-time PCR found that cows with different genetic variants of the promoter demonstrated different levels of expression of LALBA mRNA in milk somatic cells (MSCs). The TT genotype cows demonstrated low expression, whereas those with CT demonstrated much higher expression (P < 0.05). ELISA analysis found milk LALBA protein levels also differed between the TT and CT cows (P < 0.05) and that these levels were not correlated with the mRNA abundance in MSC. Association analysis found that the g.-1001T > C polymorphism in the promoter region of the LALBA gene influenced milk production traits in Polish Holstein-Friesian cows. High daily milk yield and dry matter yield, and high lactose yield and concentration were associated with the TT genotype. The TT genotype cows also had a lower number of somatic cells in the milk, considered as an indicator of udder health status. Therefore, the TT genotype could be more desirable from the breeder's perspective.
['Ostrowska|Malgorzata|M|0000-0002-1218-3225', 'Zwierzchowski|Lech|L|', 'Brzozowska|Paulina|P|', 'Kawecka-Grochocka|Ewelina|E|', 'Żelazowska|Beata|B|', 'Bagnicka|Emilia|E|']
D000818:Animals D002417:Cattle D005260:Female D005838:Genotype D007768:Lactalbumin D007774:Lactation D008892:Milk D009711:Nucleotides D020641:Polymorphism, Single Nucleotide D011401:Promoter Regions, Genetic
alpha-lactalbumin (LALBA) milk somatic cells dairy cows gene expression milk traits association promoter polymorphism