Protocol for Determining the Induction of Human Embryonic Stem Cells into Myogenic Lineage Using Electrospun Nanofibers.
An efficient method for the development of myogenic differentiation using the stem cells can be beneficial in patients with severely compromised mobility, muscular damage, or degenerative diseases. The stem cells can prove to be excellent clinical source of myogenic progenitor cells due to their ability of self-proliferation, renewal, and differentiation into a specific phenotype. They represent an essential component of tissue engineering along with other factors (e.g., 3D scaffolds, ECM mimicking environment, and growth factors). In this chapter, we describe the experimental protocols for isolation of the embryonic stem cells, their proliferation on nanofiber scaffolds, and finally their differentiation into myogenic cells. Furthermore, this chapter elaborates experimental methods to assess the myogenic fate of embryonic stem cells on the nanofiber scaffolds.
['Sofi|Hasham S|HS|', 'Khan|Nisar Ahmad|NA|', 'Sheikh|Faheem A|FA|']
D000818:Animals D002454:Cell Differentiation D019070:Cell Lineage D002469:Cell Separation D048430:Cell Shape D004622:Embryo, Mammalian D005434:Flow Cytometry D000066449:Human Embryonic Stem Cells D006801:Humans D051379:Mice D024510:Muscle Development D057139:Nanofibers D020133:Reverse Transcriptase Polymerase Chain Reaction D023822:Tissue Engineering D054457:Tissue Scaffolds
Nanofibers Myogenic differentiation Extracellular substrate Wnt3a protein