Implementation of automated testing for 1,25-dihydroxyvitamin D: Return of experience from a core-laboratory.
Measurement of 1,25(OH)2D, the most biologically active form of vitamin D, circulating levels is relevant in several physiopathological states such as chronic kidney disease, parathyroid dysfunction, sarcoidosis, and vitamin D dependent rickets. Our study determined the performances of a novel automated 1,25(OH)2D immunoassay in a core-laboratory environment. We observed satisfactory analytical performances for this assay and an excellent agreement with a well established LC-MS/MS method. Furthermore, this assay allows a reduced TAT, integration in automated core-laboratories and potential consolidation with other tests of the bone and mineral metabolism.
['Miller|Nathalie|N|', 'Gruson|Damien|D|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D057205:Automation, Laboratory D001842:Bone and Bones D002853:Chromatography, Liquid D005260:Female D006801:Humans D007118:Immunoassay D007753:Laboratories D008297:Male D008875:Middle Aged D015203:Reproducibility of Results D053719:Tandem Mass Spectrometry D014807:Vitamin D
Automation Vitamin D Kidney Bone Calcitriol Mass spectrometry