Ssm1b expression and function in germ cells of adult mice and in early embryos.
Ssm1b (Strain-specific modifier of DNA methylation 1b) is a Krüppel-associated box (KRAB) zinc finger gene that promotes CpG methylation in the mouse transgene HRD (Heavy chain enhancer, rearrangement by deletion). We report here that Ssm1b expression and concomitant HRD methylation are also present in the male and female germ cells of adult mice. Ssm1b is expressed in both diploid (2N) and haploid (1N) oocytes, as well as in 1N spermatids and spermatozoa, but not in 2N spermatogonia. Interestingly, Ssm1b mRNA is not detected in any other adult mouse organ examined, although Ssm1-family mRNAs are highly expressed in the heart. Reflecting strain specificity, Ssm1b expression and HRD methylation are not observed in early-stage C3H/HeJ mouse embryos; however, an Ssm1b-like gene that closely resembles an Ssm1b-like gene previously found in wild-derived mice is expressed in cultured embryonic stem cells derived from C3H/HeJ embryos, suggesting that culture conditions affect its expression. Collectively, this work demonstrates that HRD methylation by Ssm1b is more temporally restricted during spermatogenesis compared to oogenesis, and is altered when embryonic stem cells are cultured from C3H/HeJ inner cell mass cells.
['Ratnam|Sarayu|S|http://orcid.org/0000-0002-4771-1358', 'Bozek|Grazyna|G|', 'Martin|Terence|T|', 'Gallagher|Shannon J|SJ|', 'Payne|Christopher J|CJ|', 'Storb|Ursula|U|']
D000818:Animals D004268:DNA-Binding Proteins D004622:Embryo, Mammalian D000066473:Embryonic Germ Cells D005260:Female D018507:Gene Expression Regulation, Developmental D008297:Male D051379:Mice D009865:Oocytes D009928:Organ Specificity D013087:Spermatids D013093:Spermatogonia
DNA methylation ES cells KRAB zinc finger gene