Isolation of an indigenous Chlorella vulgaris from swine wastewater and characterization of its nutrient removal ability in undiluted sewage.
Bio-treatment of wastewater mediated by microalgae is considered as a promising solution. This work aimed to isolate an indigenous microalgal strain (named MBFJNU-1) from swine wastewater effluent and identify as Chlorella vulgaris. After 12days, the removal efficiencies of total nitrogen (TN) and total phosphorus (TP) in undiluted swine slurry were 90.51% and 91.54%, respectively. Stress tolerance in response to wastewater was verified by cultivating in artificial wastewater containing different levels of chemical oxygen demand (COD), TN and TP. MBFJNU-1 could grow well in undiluted swine slurry and artificial wastewater containing 30,000mg/L COD or 2000mg/L TN. Furthermore, global nuclear DNA methylation (5-mC) of MBFJNU-1 was employed to explore the possible mechanism in response to wastewater stress. The results showed that the level of 5-mC was inversely proportional to the growth of MBFJNU-1 in different diluted swine slurry, helping to understand 5-mC variation in response to stress environment.
['Wen|Yangmin|Y|', 'He|Yongjin|Y|', 'Ji|Xiaowei|X|', 'Li|Shaofeng|S|', 'Chen|Ling|L|', 'Zhou|Youcai|Y|', 'Wang|Mingzi|M|', 'Chen|Bilian|B|']
D000818:Animals D002708:Chlorella D048408:Chlorella vulgaris D058086:Microalgae D009584:Nitrogen D012722:Sewage D013552:Swine D014865:Waste Disposal, Fluid D062065:Waste Water
Chlorella vulgaris Swine wastewater Nutrient removal Microalgae DNA methylation