Detection of trace amounts of citrinin in dried orange peel by using an optimized extraction method coupled with ultra-performance liquid chromatography-tandem mass spectrometry.
A fast and sensitive method involving ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was introduced to detect citrinin in dried orange peel. A series of extraction, purification and chromatographic conditions was also systematically examined. With the proposed method, the obtained calibration graph was linear, with an R of 0.9996 within a concentration range of 0.5-10 ng/mL. The estimated limits of detection and quantification were 0.05 and 0.17 ng/mL, respectively. Under the selected conditions, the relative recoveries in different citrus products spiked with 1-10 ng/mL citrinin were 89.4-98.7% with RSDs of <2.5%. Compared with previously reported analytical methods, the newly developed UPLC-MS/MS method showed excellent sensitivity and good precision in detecting citrinin. The results indicated that it is a reliable and effective technique for the detection of trace citrinin in dried orange peel.
['Liu|Yanqing|Y|', 'Wang|Hongwu|H|http://orcid.org/0000-0001-8966-8653', 'Yao|Su|S|', 'Zhu|Peijie|P|']
D002851:Chromatography, High Pressure Liquid D002953:Citrinin D032084:Citrus sinensis D016014:Linear Models D010573:Pesticide Residues D015203:Reproducibility of Results D012680:Sensitivity and Specificity D052616:Solid Phase Extraction D053719:Tandem Mass Spectrometry
citrinin dried orange peel extraction UPLC-MS/MS