4-Vinylphenol production from glucose using recombinant Streptomyces mobaraense expressing a tyrosine ammonia lyase from Rhodobacter sphaeroides.
OBJECTIVES
To find a novel host for the production of 4-vinylphenol (4VPh) by screening Streptomyces species.


RESULTS
The conversion of p-coumaric acid (pHCA) to 4VPh in Streptomyces mobaraense was evaluated using a medium containing pHCA. S. mobaraense readily assimilated pHCA after 24 h of cultivation to produce 4VPh. A phenolic acid decarboxylase, derived from S. mobaraense (SmPAD), was purified following heterologous expression in Escherichia coli. SmPAD was evaluated under various conditions, and the enzyme's kcat/Km value was 0.54 mM (-1) s(-1). Using intergenetic conjugation, a gene from Rhodobacter sphaeroides encoding a tyrosine ammonia lyase, which catalyzes the conversion of L-tyrosine to p-coumaric acid, was introduced into S. mobaraense. The resulting S. mobaraense transformant produced 273 mg 4VPh l(-1) from 10 g glucose l(-1).


CONCLUSION
A novel strain suitable for the production of 4VPh and potentially other aromatic compounds was isolated.
['Fujiwara|Ryosuke|R|', 'Noda|Shuhei|S|', 'Kawai|Yoshifumi|Y|', 'Tanaka|Tsutomu|T|', 'Kondo|Akihiko|A|']
D000642:Ammonia-Lyases D003373:Coumaric Acids D005947:Glucose D010636:Phenols D011422:Propionates D012242:Rhodobacter sphaeroides D013302:Streptomyces
4-Vinylphenol Streptomyces mobaraense p-Coumaric acid Phenolic acids Intergenetic conjugation