Mytilus galloprovincialis (Lamarck, 1819) spermatozoa: hsp70 expression and protamine-like protein property studies.
In this work, we describe results of the reproductive health monitoring studies in Mytilus galloprovincialis following spermatozoa hsp70 expression and protamine-like protein properties. Mussels control (ctr) were released within cages for 30 days in three different marine sites near Naples (Campania, Italy): Bagnoli south (BAs) and Bagnoli north (BAn), both close to a disposal metallurgical factory and in Capo Miseno (CM). Studies of hsp70 gene expression carried out, by RT-qPCR, in mussel spermatozoa have shown varied expression levels, particularly 5, 13, and 15-fold more than ctr in CM, BAs, and BAn, respectively, indicating highest involvement of stress proteins in spermatozoa of mussels in Bagnoli. In order to evaluate the possible risk on Mytilus galloprovincialis sustainability loss, electrophoretic analyses were performed on protamine-like proteins (PL) of collected spermatozoa. The results showed that CM PL were apparently unaltered with respect to ctr PL, while BAs and BAn PL appeared in part in the form of peptides and in part as bands with low mobility. Further, CM and BAs PL showed, by electrophoretic mobility shift assay, a decrease in DNA binding ability and a change in their DNA binding mode. The results of this investigation show the usefulness of the study of alterations of spermatozoa hsp70 expression and protamine-like protein properties for eco-toxicological evaluation using Mytilus galloprovincialis as a bioindicator.
['Piscopo|Marina|M|', 'Notariale|Rosaria|R|', 'Rabbito|Dea|D|', 'Ausió|Juan|J|', 'Olanrewaju|Oladokun Sulaiman|OS|', 'Guerriero|Giulia|G|']
D000818:Animals D024202:Electrophoretic Mobility Shift Assay D004784:Environmental Monitoring D018840:HSP70 Heat-Shock Proteins D007558:Italy D008297:Male D008669:Metallurgy D049878:Mytilus D011479:Protamines D013094:Spermatozoa D014876:Water Pollution
Mytilus galloprovincialis Spermatozoa hsp70 Protamine-like proteins Bagnoli Capo Miseno