Structure and anticancer activity of native and modified polysaccharides from brown alga Dictyota dichotoma.
The laminaran DdL and fucoidan DdF were obtained from the brown alga Dictyota dichotoma. DdF was a sulfated (28.9%) and acetylated heteropolysaccharide containing fucose, galactose, mannose and glucose (57.9, 20.4, 12.4 and 9.2mol%, respectively). DdL was a 1,3;1,6-β-d-glucan with the main chain built from 1,3-linked glucose residues and single glucose residue in branches at C6 (one branch on three glucose residues of the main chain). Sulfated (43.7%) laminaran DdLs was obtained from DdL by sulfation. It was determined that sulfates occur at C2, C4 and C6 of glucose residues. The anticancer effect of DdF, DdL, and DdLs (200μg/mL) was studied in vitro on colon cancer cells HCT-116, HT-29, and DLD-1. The effect of polysaccharides (40μg/mL) on colony formation of DLD-1 cancer cells after irradiation (4Gy) was investigated first. All polysaccharides showed a synergistic effect with X-ray irradiation against cancer cells, decreasing the amount and size of cancer cells colonies.
['Usoltseva|Roza V|RV|', 'Shevchenko|Natalia M|NM|', 'Malyarenko|Olesya S|OS|', 'Ishina|Irina A|IA|', 'Ivannikova|Svetlana I|SI|', 'Ermakova|Svetlana P|SP|']
D000970:Antineoplastic Agents D049109:Cell Proliferation D005936:Glucans D045325:HCT116 Cells D019073:HT29 Cells D006801:Humans D000459:Phaeophyta D011134:Polysaccharides
Structure Anticancer activity Dictyota Laminaran Fucoidan γ-radiation