Over-expression of carbon monoxide dehydrogenase-I with an accessory protein co-expression: a key enzyme for carbon dioxide reduction.
Carbon monoxide dehydrogenase-I (CODH-I) from the CO-utilizing bacterium Carboxydothermus hydrogenoformans are expected to be utilized as a part of reproducible carbon dioxide photoreduction system. However, the over-expression system for CODH-I remains to be constructed. CODH-I constitutes a hydrogenase/CODH gene cluster including a gene encoding a Ni-insertion accessory protein, CooC (cooC3). Through co-expression of CooC3, we found an over-expression system with higher activity. The Rec-CODH-I with the co-expression exhibits 8060 U/mg which was approximately threefold than that without co-expression (2270 U/mg). In addition, co-expression resulted in Ni(2+) content increase; the amount of Ni atoms of Rec-CODH-I was approximately thrice than that without co-expression.
['Inoue|Takahiro|T|', 'Takao|Kyousuke|K|', 'Fukuyama|Yuto|Y|', 'Yoshida|Takashi|T|', 'Sako|Yoshihiko|Y|']
D000445:Aldehyde Oxidoreductases D000595:Amino Acid Sequence D001426:Bacterial Proteins D002245:Carbon Dioxide D015870:Gene Expression D005818:Genetic Engineering D008969:Molecular Sequence Data D009097:Multienzyme Complexes D009532:Nickel D010084:Oxidation-Reduction D011994:Recombinant Proteins
CODH CooC C-cluster carbon monoxide dehydrogenase