New denture adhesive containing miconazole nitrate polymeric microparticles: Antifungal, adhesive force and toxicity properties.
OBJECTIVE
The purpose of this study was to develop a new oral drug delivery system by incorporating polymeric miconazole nitrate (MN) microparticles on an experimental antifungal denture adhesive (DA).


METHODS
Spray drying Eudragit L-100 (E) and Gantrez MS-955 (G) MN-microparticles were incorporated in DA. DAE1, DAG1, DAEG1, DAE2, DAG2, DAEG2 groups were obtained from the combination of polymers used in MN-microparticles (E, G and EG) and concentration of MN into DA (1, for 1% and 2, for 2%). DA with 2% pure MN (DAM) and DA without microparticles or drug (DACT) were both control groups. All groups were evaluated to determine microbiological assay, adhesive force and toxicity. Minimum inhibitory concentration (MIC) against Candida albicans was performed by broth micro-dilution and agar dilution methods in extract of DAs and conventional gel form (Daktarin®). Adhesive load testing was made between acrylic resin samples on a universal testing machine after immersion in water. The toxicity of several dilutions of DAs was performed with Artemia salina bioassay after 24 and 48h. Data of adhesive force were evaluated with two-way ANOVA and Bonferroni tests (α=0.05).


RESULTS
The concentration required to kill 50% (LC50) was determined using the Provit analysis. DA with polymeric microparticles and pure drug presented MIC between 1.25-5μg/mL similar to MIC values of DAM. DAEG2, DAEG1, DAG20 showed the most actives against C. albicans. The best adhesive properties were exhibited by DAEG2, consisting of high initial adhesive force which was maintained for up to 6h. The extracts of all DA presented low or not toxicity at 24 and 48h.


SIGNIFICANCE
DA containing 2% of MN loaded in microparticles made by Gantrez MS-955 alone or combined with Eudragit L-100 produce effective antifungal activity, good adhesive force, and no toxicity effect being a promising therapeutics for removable denture wearers affected by denture stomatitis.
['Cartagena|Andrés Felipe|AF|', 'Esmerino|Luís Antonio|LA|', 'Polak-Junior|Rogerio|R|', 'Olivieri Parreiras|Sibelli|S|', 'Domingos Michél|Milton|M|', 'Farago|Paulo Vitor|PV|', 'Campanha|Nara Hellen|NH|']
D000180:Acrylic Resins D000269:Adhesives D000935:Antifungal Agents D002176:Candida albicans D003738:Dental Cements D003819:Denture Bases D003781:Denture Retention D003778:Dentures D008825:Miconazole D011109:Polymethacrylic Acids
Denture adhesives Miconazole nitrate Denture stomatitis Complete denture Microparticles drug delivery