Light Energy Conversion Surface with Gold Dendritic Nanoforests/Si Chip for Plasmonic Polymerase Chain Reaction.
Surfaces with gold dendritic nanoforests (Au DNFs) on Si chips demonstrate broadband-light absorption. This study is the first to utilize localized surface plasmons of Au DNFs/Si chips for polymerase chain reaction (PCR) applications. A convenient halogen lamp was used as the heating source to illuminate the Au DNFs/Si chip for PCR. A detection target of Salmonella spp. DNA fragments was reproduced in this plasmonic PCR chip system. By semi-quantitation in gel electrophoresis analysis, the plasmonic PCR with 30 cycles and a largely reduced processing time provided results comparable with those of a commercial PCR thermal cycler with 40 cycles in more than 1 h. In the presence of an Au DNFs/Si chip, the plasmonic PCR provides superior results in a short processing time.
['Huang|Hung Ji|HJ|0000-0003-3753-0067', 'Chiang|Yu-Cheng|YC|', 'Hsu|Chia-Hsien|CH|', 'Chen|Jyh-Jian|JJ|', 'Shiao|Ming-Hua|MH|', 'Yeh|Chih-Chieh|CC|', 'Huang|Shu-Ling|SL|', 'Lin|Yung-Sheng|YS|0000-0002-9522-1812']
gold dendritic plasmon polymerase chain reaction Salmonella spp. DNA silicon