An in vivo comparison of antimicrobial efficacy of sodium hypochlorite and Biopure MTAD™ against enterococcus faecalis in primary teeth: A qPCR study.
OBJECTIVES
Biopure MTAD™, a new root canal irrigant has shown promising results against the most common resistant microorganism, E. faecalis, in permanent teeth. However, there is lack of studies comparing its antimicrobial effectiveness with NaOCl in primary teeth. The purpose of this study was to compare the in vivo antimicrobial efficacy of NaOCl 2.5% and Biopure MTAD™ against E. faecalis in primary teeth.


STUDY DESIGN
Forty non vital single rooted primary maxillary anterior teeth of children aged 4-8 years, were irrigated either with NaOCl 2.5% (n=15), Biopure MTAD™ (n=15) and 0.9% Saline (n=10, control group). Paper point samples were collected at baseline (S1) and after chemomechanical preparation (S2) during the pulpectomy procedure. The presence of E. faecalis in S1 & S2 was evaluated using Real time Polymerase Chain Reaction.


RESULTS
Statistical significant difference was found in the antimicrobial efficacy of NaOCl 2.5 % and BioPure MTAD™ when compared to saline (p>0.05). However, no statistical significant difference was found between the efficacies of both the irrigants.


CONCLUSIONS
NaOCl 2.5% and BioPure MTAD™, both irrigants are equally efficient against E. faecalis in necrotic primary anterior teeth. MTAD is a promising irrigant, however clinical studies are required to establish it as ideal root canal irrigant in clinical practice.
['Tulsani|S G|SG|', 'Chikkanarasaiah|N|N|', 'Bethur|S|S|']
D000891:Anti-Infective Agents, Local D002648:Child D002675:Child, Preschool D019343:Citric Acid D003786:Dental Pulp Cavity D003790:Dental Pulp Necrosis D004318:Doxycycline D013293:Enterococcus faecalis D006801:Humans D011136:Polysorbates D011670:Pulpectomy D060888:Real-Time Polymerase Chain Reaction D012388:Root Canal Irrigants D018915:Root Canal Preparation D012973:Sodium Hypochlorite D014094:Tooth, Deciduous
Sodium hypochlorite Biopure MTAD Primary teeth Irrigant Pulpectomy Endodontic treatment