Role of colchicine-induced microtubule depolymerization in hyperalgesia via TRPV4 in rats with chronic compression of the dorsal root ganglion.
The aim of this study is to investigate the effect of microtubule depolymerization by colchicine on hyperalgesia mediated by transient receptor potential vanilloid 4 (TRPV4) in a neuropathic pain model of chronic compression of the dorsal root ganglion (DRG) (hereafter termed CCD) in rat. Intrathecal administration of microtubule-depolymerizing agent, colchicine, attenuated the activated effect of 4alpha-phorbol 12, 13-didecanoate (4alpha-PDD, TRPV4 specific agonist) on mechanical and thermal hyperalgesia in CCD rats. This observation is in agreement with our in vitro experiments with DRG cells that showed a significant attenuation of 4alpha-PDD-activated Ca(2+)-influx and substance P (SP) release with the colchicine treatment. We conclude that microtubule depolymerization by colchicine can regulate pain sensitivity by depressing the hyperalgesia mediated by TRPV4.
['Ning|Liping|L|', 'Wang|Chuanwei|C|', 'Fan|Xiaohua|X|', 'Ding|Xinli|X|', 'Wang|Yonghui|Y|', 'Zhang|Yang|Y|', 'Wang|Jie|J|', 'Yue|Shouwei|S|']
D000818:Animals D002118:Calcium D002470:Cell Survival D003078:Colchicine D005727:Ganglia, Spinal D006358:Hot Temperature D006930:Hyperalgesia D008297:Male D008870:Microtubules D009408:Nerve Compression Syndromes D009437:Neuralgia D011843:Radiculopathy D011897:Random Allocation D051381:Rats D017208:Rats, Wistar D013373:Substance P D050916:TRPV Cation Channels D014110:Touch D050257:Tubulin Modulators
CCD Colchicine, Hyperalgesia, Microtubule depolymerization, Transient receptor potential vanilloid 4,