Proteomics reveals localization of cuticular proteins in Anopheles gambiae.
Anopheles gambiae devotes over 2% of its protein coding genes to its 298 structural cuticular proteins (CPs). This paper provides new LC-MS/MS data on two adult structures, proboscises and palps, as well as three larval samples - 4th instar larvae, just their terminal segment, and a preparation enriched in their tracheae. These data were combined with our previously published results of proteins from five other adult structures, whole adults, and two preparations chosen for their relatively clean cuticle, the larval head capsules left behind after ecdysis and the pupal cuticles left behind after adult eclosion. Peptides from 28 CPs were recovered in all adult structures; 24 CPs were identified for the first time, 6 of these were members of the TWDL family. Most newly identified proteins came from the larval sources. Based solely on peptide recovery, from our data and from other investigators, most available on VectorBase, there were only 4 CPs that were restricted to a single adult structure. More were restricted to a single metamorphic stage, 14 in larvae, 0 in pupae and 32 in adults. Expression data from our earlier RT-qPCR studies reduces these numbers. Charting restriction of CPs to stage or structure is a step forward in establishing their specific roles.
['Zhou|Yihong|Y|', 'Badgett|Majors J|MJ|', 'Orlando|Ron|R|', 'Willis|Judith H|JH|']
D000818:Animals D000852:Anopheles D019476:Insect Proteins D007814:Larva D018978:Molting D040901:Proteomics D011679:Pupa
LC-MS/MS Palp Larvae Tracheae Proboscis