Extracellular pyruvate kinase M2 facilitates cell migration by upregulating claudin-1 expression in colon cancer cells.
Extensive studies have been reported the non-canonical functions of pyruvate kinase M2 (PKM2) as a kinase, transcriptional regulator, and even cell-to-cell communicator, emphasizing its importance in various signaling pathways. However, the role of secreted PKM2 in cancer progression and its signaling pathway is yet to be elucidated. In this study, we found that extracellular PKM2 enhanced the migration of low-metastatic, benign colon cancer cells by upregulating claudin-1 expression and internalizing it to the cytoplasm and nucleus. Knock-down of claudin-1 significantly reduced extracellular PKM2-induced cell migration. Inhibition of either protein kinase C (PKC) or epidermal growth factor receptor (EGFR) resulted in a reduction of extracellular PKM2-mediated claudin-1 expression, suggesting EGFR-PKC-claudin-1 as a signaling pathway in the extracellular PKM2-mediated tumorigenesis of colon cancer cells.
['Kim|Hyunju|H|', 'Kim|Seong Ho|SH|', 'Hwang|Dohyeon|D|', 'An|Jinsu|J|', 'Chung|Hak Suk|HS|', 'Yang|Eun Gyeong|EG|', 'Kim|So Yeon|SY|']
D018938:Caco-2 Cells D002352:Carrier Proteins D045744:Cell Line, Tumor D002465:Cell Movement D002467:Cell Nucleus D062445:Claudin-1 D003110:Colonic Neoplasms D003593:Cytoplasm D018450:Disease Progression D066246:ErbB Receptors D015972:Gene Expression Regulation, Neoplastic D045325:HCT116 Cells D006801:Humans D008565:Membrane Proteins D011493:Protein Kinase C D034622:RNA Interference D015398:Signal Transduction D013963:Thyroid Hormones
cell migration claudin-1 colon cancer extracellular PKM2 PKC EGFR PKM2 extracellulaire cancer du côlon claudine-1 migration cellulaire