Effect of 2-aminoethoxydiphenyl borate on ischemia-reperfusion injury in a rat ovary model.
OBJECTIVE
The aim of this study is to evaluate the effects of 2-aminoethoxydiphenyl borate (2-APB) as an antioxidant and analyze biochemical and histopathologic changes in experimental ischemia-reperfusion (I/R) injury in rat ovaries.


STUDY DESIGN
Thirty female rats were utilized to create four groups. Group 1: I/R and 2-APB (2mg/kg); Group 2: I/R and 2-APB (4mg/kg); Group 3: I/R; Group 4: sham operation. Ovarian tissue and serum malondialdehyde, nitric oxide (NO) levels; ovarian tissue and serum total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) were determined. In ovarian tissue samples histopathologic examination, immunoflourescence staining by TUNEL method was studied.


RESULTS
Tissue TOS, serum TOS, and OSI levels were elevated in I/R group. After treatment with 2-APB, tissue and serum TOS levels and OSI levels were markedly decreased. There was a significant difference in terms of tissue and serum NO levels between the sham group and I/R group. Elevation in tissue NO and serum NO levels were decreased after treatment with 2-APB. TUNEL-positive cell number gradually decreased with dose of 2-APB in groups 1 and 2.


CONCLUSION
Conservative treatment with 2-APB is beneficial for mitigation of I/R injury, and the ovarian protective effect of 2-APB appears to be mediated through its antiapopitotic and antioxidative effects.
['Taskin|Mine Islimye|MI|', 'Hismiogullari|Adnan Adil|AA|', 'Yay|Arzu|A|', 'Adali|Ertan|E|', 'Gungor|Aysenur Cakir|AC|', 'Korkmaz|Gozde Ozge|GO|', 'Inceboz|Umit|U|']
D000818:Animals D000975:Antioxidants D017209:Apoptosis D001896:Boron Compounds D005260:Female D009569:Nitric Oxide D010049:Ovarian Diseases D010053:Ovary D018384:Oxidative Stress D017208:Rats, Wistar D015427:Reperfusion Injury D054159:Torsion, Mechanical
Ischemia-reperfusion Rat 2-Aminoethoxydiphenyl borate (2-APB) Adnexal torsion