Distinct modes of recruitment of the CCR4-NOT complex by Drosophila and vertebrate Nanos.
Nanos proteins repress the expression of target mRNAs by recruiting effector complexes through non-conserved N-terminal regions. In vertebrates, Nanos proteins interact with the NOT1 subunit of the CCR4-NOT effector complex through a NOT1 interacting motif (NIM), which is absent in Nanos orthologs from several invertebrate species. Therefore, it has remained unclear whether the Nanos repressive mechanism is conserved and whether it also involves direct interactions with the CCR4-NOT deadenylase complex in invertebrates. Here, we identify an effector domain (NED) that is necessary for the Drosophila melanogaster (Dm) Nanos to repress mRNA targets. The NED recruits the CCR4-NOT complex through multiple and redundant binding sites, including a central region that interacts with the NOT module, which comprises the C-terminal domains of NOT1-3. The crystal structure of the NED central region bound to the NOT module reveals an unanticipated bipartite binding interface that contacts NOT1 and NOT3 and is distinct from the NIM of vertebrate Nanos. Thus, despite the absence of sequence conservation, the N-terminal regions of Nanos proteins recruit CCR4-NOT to assemble analogous repressive complexes.
['Raisch|Tobias|T|', 'Bhandari|Dipankar|D|', 'Sabath|Kevin|K|', 'Helms|Sigrun|S|', 'Valkov|Eugene|E|', 'Weichenrieder|Oliver|O|', 'Izaurralde|Elisa|E|']
D000818:Animals D002352:Carrier Proteins D018360:Crystallography, X-Ray D029721:Drosophila Proteins D004331:Drosophila melanogaster D011485:Protein Binding D011487:Protein Conformation D012333:RNA, Messenger D016601:RNA-Binding Proteins D012260:Ribonucleases
deadenylation decapping mRNA decay translational repression