Vanilla bahiana, a contribution from the Atlantic Forest biodiversity for the production of vanilla: A proteomic approach through high-definition nanoLC/MS.
Only a few cultivated species of Vanilla are used to produce vanilla, despite the high demand, predatory exploitation, and low genetic variability that threaten the production of natural vanilla. Vanilla bahiana pods from the Atlantic Forest may be an alternative source of natural vanilla. This study applied bottom-up and shotgun proteomics analysis to identify proteins related to flowering, fruiting, and vanilla-flavor production. Extraction solutions, including Tris-HCl buffer, β-mercaptoethanol and SDS, were assayed. SDS proved to be feasible for extraction of Vanilla fruit proteins and could be an alternative to the phenol method of protein extraction. Progenesis QI for Proteomics (QIP) software loaded with an Orchidaceae database identified 2326 proteins in our samples. Among these, 75 were highlighted as useful for the synthesis of compounds related to vanilla flavor, such as vanillin synthase, which was successfully extracted with 1% SDS, which also improved the variety of the extracted proteins. The proteins identified in V. bahiana pods indicate the enzymatic potential of this species, as further validated by quantifying the vanilla in the samples.
['Lopes|Ellen Moura|EM|', 'Linhares|Roberta Gomes|RG|', 'de Oliveira Pires|Lucas|L|', 'Castro|Rosane Nora|RN|', 'Souza|Gustavo Henrique Martins Ferreira|GHMF|', 'Koblitz|Maria Gabriela Bello|MGB|', 'Cameron|Luiz Claudio|LC|', 'Macedo|Andrea Furtado|AF|']
D001547:Benzaldehydes D044822:Biodiversity D005421:Flavoring Agents D019649:Food Industry D065928:Forests D005638:Fruit D006801:Humans D010936:Plant Extracts D010940:Plant Proteins D040901:Proteomics D021241:Spectrometry, Mass, Electrospray Ionization D031669:Vanilla
β-Mercaptoethanol Vanillin Vanillin synthase Bottom up Ion mobility Sodium dodecyl sulfate nanoUPLC/HDMS(E)