Genome-wide characterization and comparative analysis of the MLO gene family in cotton.
In plants, MLO (Mildew Locus O) gene encodes a plant-specific seven transmembrane (TM) domain protein involved in several cellular processes, including susceptibility to powdery mildew (PM). In this study, a genome-wide characterization of the MLO gene family in G. raimondii L., G. arboreum L. and G. hirsutum L. was performed. In total, 22, 17 and 38 homologous sequences were identified for each species, respectively. Gene organization, including chromosomal location, gene clustering and gene duplication, was investigated. Homologues related to PM susceptibility in upland cotton were inferred by phylogenetic relationships with functionally characterized MLO proteins. To conduct a comparative analysis between MLO candidate genes from G. raimondii L., G. arboreum L. and G. hirsutum L., orthologous relationships and conserved synteny blocks were constructed. The transcriptional variation of 38 GhMLO genes in response to exogenous application of salt, mannitol (Man), abscisic acid (ABA), ethylene (ETH), jasmonic acid (JA) and salicylic acid (SA) was monitored. Further studies should be conducted to elucidate the functions of MLO genes in PM susceptibility and phytohormone signalling pathways.
['Wang|Xiaoyan|X|', 'Ma|Qifeng|Q|', 'Dou|Lingling|L|', 'Liu|Zhen|Z|', 'Peng|Renhai|R|', 'Yu|Shuxun|S|']
D000595:Amino Acid Sequence D020440:Gene Duplication D018507:Gene Expression Regulation, Developmental D018506:Gene Expression Regulation, Plant D018745:Genome, Plant D003368:Gossypium D005810:Multigene Family D010802:Phylogeny D010937:Plant Growth Regulators D018515:Plant Leaves D016415:Sequence Alignment D026801:Synteny
MLO Gene family Synteny blocks Phytohormone Abiotic stress Gossypium