Simultaneous quantification of polyphenols, glycoalkaloids and saponins in African nightshade leaves using ultra-high performance liquid chromatography tandem mass spectrometry with acid assisted hydrolysis and multivariate analysis.
This study developed comprehensive quantification methods for major nutritive and antinutritive phytochemical aglycones in edible African nightshade leaves, an underutilized food resource in the sub-Saharan area. A simultaneous hydrolysis and extraction method was developed using methanol with 2 M sulfuric acid with incubation at 65 °C for 60 min. UHPLC-QqQ-MS/MS methods were developed and validated for hydrolysis optimization and for quantification of eight major aglycones of polyphenols, alkaloids and sapogenins in 20 differently sourced nightshade leaves, comprising two African species Solanum scabrum and S. nigrum, and from two distinct cultivation sites, one in New Jersey, US and the other in Kenya Eldoret. Variation in species, accessions and cultivation environment played an important role in affecting the phytochemical profile. Total antinutritive alkaloids and sapogenins in all nightshade leaves were evaluated and found to be safe for consumption. This work provides evidence that the consumption of African nightshade leaves as a nutrient rich leafy green vegetable is safe and can contribute to food security and nutritional improvement in the sub-Saharan area.
['Yuan|Bo|B|', 'Dinssa|Fekadu F|FF|', 'Simon|James E|JE|', 'Wu|Qingli|Q|']
D000470:Alkaloids D002851:Chromatography, High Pressure Liquid D006031:Glycosylation D006868:Hydrolysis D007630:Kenya D015999:Multivariate Analysis D018515:Plant Leaves D059808:Polyphenols D012503:Saponins D032322:Solanum D053719:Tandem Mass Spectrometry
Glycoalkaloids Hydrolysis Solanum scabrum African indigenous vegetable Principle component analysis Solanum nigrum UHPLC–MS/MS chlorogenic acid (PubChem CID:1794427) dioscin (PubChem CID: 119245) diosgenin (PubChem CID: 99474) isoquercetin (PubChem CID: 5280804) isorhamnetin (PubChem CID: 5281654) kaempferol (PubChem CID: 5280863) quercetin (PubChem CID: 5280343) rhamnetin (PubChem CID: 5281691) solamargine (PubChem CID: 73611) solasodine (PubChem CID: 442985) tigogenin (PubChem CID: 99516)