Response of denitrifying community, denitrification genes and antibiotic resistance genes to oxytetracycline stress in polycaprolactone supported solid-phase denitrification reactor.
The coexistence of nitrate and antibiotics in wastewater is a common problem. The study aimed to explore the response of denitrifying community, denitrification genes and antibiotic resistance genes (ARGs) to oxytetracycline (OTC) stress in polycaprolactone (PCL) supported solid-phase denitrification (SPD) reactors. Complete nitrate reduction (greater than99%) was achieved in SPD system with OTC stress of 0, 0.05, 0.25 and 1 mg L-1 during three-month operation, while it significantly declined by about 5% at a further increased OTC level of 5 mg L-1. The efficient denitrification strongly related with a rich diversity of denitrifiers, while the abundances of which dramatically reduced as the OTC concentration reached ≥0.25 mg L-1, which caused significant decline of denitrification genes, especially for narH, narJ, narI nirD, nosZ, and norB. Tetracycline resistance genes were a major type of promoted ARGs by different OTC stress, mainly related with the increase of tet36, tetG, tetA, tetM and tetC.
['Feng|Lijuan|L|', 'Yang|Jingyi|J|', 'Yu|Hui|H|', 'Lan|Zeyu|Z|', 'Ye|Xin|X|', 'Yang|Guangfeng|G|', 'Yang|Qiao|Q|', 'Zhou|Jiaheng|J|']
D000900:Anti-Bacterial Agents D058440:Denitrification D004352:Drug Resistance, Microbial D010118:Oxytetracycline D011091:Polyesters
Denitrification genes Antibiotic resistance genes (ARGs) Oxytetracycline (OTC) Solid-phase denitrification (SPD) Microbial community structure