Reverse Plasticity Underlies Rapid Evolution by Clonal Selection within Populations of Fibroblasts Propagated on a Novel Soft Substrate.
Mechanical properties such as substrate stiffness are a ubiquitous feature of a cell's environment. Many types of animal cells exhibit canonical phenotypic plasticity when grown on substrates of differing stiffness, in vitro and in vivo. Whether such plasticity is a multivariate optimum due to hundreds of millions of years of animal evolution, or instead is a compromise between conflicting selective demands, is unknown. We addressed these questions by means of experimental evolution of populations of mouse fibroblasts propagated for approximately 90 cell generations on soft or stiff substrates. The ancestral cells grow twice as fast on stiff substrate as on soft substrate and exhibit the canonical phenotypic plasticity. Soft-selected lines derived from a genetically diverse ancestral population increased growth rate on soft substrate to the ancestral level on stiff substrate and evolved the same multivariate phenotype. The pattern of plasticity in the soft-selected lines was opposite of the ancestral pattern, suggesting that reverse plasticity underlies the observed rapid evolution. Conversely, growth rate and phenotypes did not change in selected lines derived from clonal cells. Overall, our results suggest that the changes were the result of genetic evolution and not phenotypic plasticity per se. Whole-transcriptome analysis revealed consistent differentiation between ancestral and soft-selected populations, and that both emergent phenotypes and gene expression tended to revert in the soft-selected lines. However, the selected populations appear to have achieved the same phenotypic outcome by means of at least two distinct transcriptional architectures related to mechanotransduction and proliferation.
['Purkayastha|Purboja|P|0000-0002-4895-161X', 'Pendyala|Kavya|K|', 'Saxena|Ayush S|AS|0000-0002-1190-3905', 'Hakimjavadi|Hesamedin|H|', 'Chamala|Srikar|S|', 'Dixit|Purushottam|P|', 'Baer|Charles F|CF|0000-0002-0140-5814', 'Lele|Tanmay P|TP|']
D000222:Adaptation, Physiological D000818:Animals D005075:Biological Evolution D005347:Fibroblasts D015870:Gene Expression D040961:Genetic Drift D040542:Mechanotransduction, Cellular D051379:Mice D041681:NIH 3T3 Cells D012641:Selection, Genetic
phenotypic plasticity experimental evolution gene expression mechanotransduction evolutionary cell biology