The α-cyclodextrin complex of the Moringa isothiocyanate suppresses lipopolysaccharide-induced inflammation in RAW 264.7 macrophage cells through Akt and p38 inhibition.
In the last decades, a growing need to discover new compounds for the prevention and treatment of inflammatory diseases has led researchers to consider drugs derived from natural products as a valid option in the treatment of inflammation-associated disorders. The purpose of the present study was to investigate the anti-inflammatory effects of a new formulation of Moringa oleifera-derived 4-(α-L-rhamnopyranosyloxy)benzyl isothiocyanate as a complex with alpha-cyclodextrin (moringin + α-CD) on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells, a common model used for inflammation studies. In buffered/aqueous solution, the moringin + α-CD complex has enhanced the water solubility and stability of this isothiocyanate by forming a stable inclusion system. Our results showed that moringin + α-CD inhibits the production of inflammatory mediators in LPS-stimulated macrophages by down-regulation of pro-inflammatory cytokines (TNF-α and IL-1β), by preventing IκB-α phosphorylation, translocation of the nuclear factor-κB (NF-κB), and also via the suppression of Akt and p38 phosphorylation. In addition, as a consequence of upstream inhibition of the inflammatory pathway following treatment with moringin + α-CD, the modulation of the oxidative stress (results focused on the expression of iNOS and nitrotyrosine) and apoptotic pathway (Bax and Bcl-2) was demonstrated. Therefore, moringin + α-CD appears to be a new relevant helpful tool to use in clinical practice for inflammation-associated disorders.
['Giacoppo|Sabrina|S|', 'Rajan|Thangavelu Soundara|TS|', 'Iori|Renato|R|', 'Rollin|Patrick|P|', 'Bramanti|Placido|P|', 'Mazzon|Emanuela|E|']
D000818:Animals D000893:Anti-Inflammatory Agents D007249:Inflammation D053583:Interleukin-1beta D017879:Isothiocyanates D008070:Lipopolysaccharides D051379:Mice D029587:Moringa D000072000:NF-KappaB Inhibitor alpha D016328:NF-kappa B D051057:Proto-Oncogene Proteins c-akt D000067996:RAW 264.7 Cells D014409:Tumor Necrosis Factor-alpha D047391:alpha-Cyclodextrins D048051:p38 Mitogen-Activated Protein Kinases
Moringa isothiocyanate Inflammation RAW 264.7 macrophage cells Akt P38 α-CD-complexed moringin