Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment. We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study. The results showed that our DOX-SPIO-CS/CHI MPs had an average diameter of 1.43±0.54μm and exhibited a spherical shape. The encapsulation efficiency of this drug carrier was approximately 31±8.07%, according to our spectroscopic determination. The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period. According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
['Tang|Sung-Ling|SL|', 'Bai|Meng-Yi|MY|', 'Wang|Jyun-Yi|JY|', 'Hong|Po-Da|PD|']
D000818:Animals D006528:Carcinoma, Hepatocellular D045744:Cell Line, Tumor D002470:Cell Survival D048271:Chitosan D002809:Chondroitin Sulfates D004317:Doxorubicin D004337:Drug Carriers D005260:Female D005290:Ferric Compounds D056945:Hep G2 Cells D006801:Humans D008113:Liver Neoplasms D051379:Mice D008807:Mice, Inbred BALB C D008819:Mice, Nude D046529:Microscopy, Electron, Transmission D008863:Microspheres D009368:Neoplasm Transplantation D010316:Particle Size D011134:Polysaccharides D013053:Spectrophotometry
Doxorubicin Microparticles Chitosan Electrospray Nanotechnology SPIO