SMAD3 and SP1/SP3 Transcription Factors Collaborate to Regulate Connective Tissue Growth Factor Gene Expression in Myoblasts in Response to Transforming Growth Factor β.
Fibrotic disorders are characterized by an increase in extracellular matrix protein expression and deposition, Duchene Muscular Dystrophy being one of them. Among the factors that induce fibrosis are Transforming Growth Factor type β (TGF-β) and the matricellular protein Connective Tissue Growth Factor (CTGF/CCN2), the latter being a target of the TGF-β/SMAD signaling pathway and is the responsible for the profibrotic effects of TGF-β. Both CTGF and TGF are increased in tissues affected by fibrosis but little is known about the regulation of the expression of CTGF mediated by TGF-β in muscle cells. By using luciferase reporter assays, site directed mutagenesis and specific inhibitors in C2C12 cells; we described a novel SMAD Binding Element (SBE) located in the 5' UTR region of the CTGF gene important for the TGF-β-mediated expression of CTGF in myoblasts. In addition, our results suggest that additional transcription factor binding sites (TFBS) present in the 5' UTR of the CTGF gene are important for this expression and that SP1/SP3 factors are involved in TGF-β-mediated CTGF expression.
['Córdova|Gonzalo|G|', 'Rochard|Alice|A|', 'Riquelme-Guzmán|Camilo|C|', 'Cofré|Catalina|C|', 'Scherman|Daniel|D|', 'Bigey|Pascal|P|', 'Brandan|Enrique|E|']
D020121:5' Untranslated Regions D000818:Animals D001665:Binding Sites D002460:Cell Line D055513:Connective Tissue Growth Factor D005786:Gene Expression Regulation D051379:Mice D016297:Mutagenesis, Site-Directed D032446:Myoblasts D015398:Signal Transduction D051900:Smad3 Protein D016329:Sp1 Transcription Factor D051705:Sp3 Transcription Factor D016212:Transforming Growth Factor beta
FIBROSIS CTGF/CCN2 SMAD DUCHENNE MUSCULAR DYSTROPHY SKELETAL MUSCLE TGF-BETA