Effect of Kombucha, a fermented black tea in attenuating oxidative stress mediated tissue damage in alloxan induced diabetic rats.
Diabetic complications associated with increased oxidative stress can be suppressed by antioxidants. In the present study we investigated the antidiabetic and antioxidant effects of Kombucha (KT), a fermented black tea, in comparison to that of unfermented black tea (BT), in ALX-induced diabetic rats. ALX exposure lowered the body weight and plasma insulin by about 28.12% and 61.34% respectively and elevated blood glucose level and glycated Hb by about 3.79 and 3.73 folds respectively. The oxidative stress related parameters like lipid peroxidation end products (increased by 3.38, 1.7, 1.65, 1.94 folds respectively), protein carbonyl content (increased by 2.5, 2.35, 1.8, 3.26 folds respectively), glutathione content (decreased by 59.8%, 47.27%, 53.69%, 74.03% respectively), antioxidant enzyme activities were also altered in the pancreatic, hepatic, renal and cardiac tissues of diabetic animals. Results showed significant antidiabetic potential of the fermented beverage (150 mg lyophilized extract/kg bw for 14 days) as it effectively restored ALX-induced pathophysiological changes. Moreover, it could ameliorate DNA fragmentation and caspase-3 activation in the pancreatic tissue of diabetic rats. Although unfermented black tea is effective in the above pathophysiology, KT was found to be more efficient. This might be due to the formation of some antioxidant molecules during fermentation period.
['Bhattacharya|Semantee|S|', 'Gachhui|Ratan|R|', 'Sil|Parames C|PC|']
D000496:Alloxan D000818:Animals D000975:Antioxidants D001786:Blood Glucose D028241:Camellia sinensis D053148:Caspase 3 D002784:Cholesterol D053938:DNA Fragmentation D003921:Diabetes Mellitus, Experimental D005285:Fermentation D005419:Flavonoids D005978:Glutathione D005979:Glutathione Peroxidase D006454:Hemoglobins D007328:Insulin D007668:Kidney D015227:Lipid Peroxidation D008099:Liver D008297:Male D018384:Oxidative Stress D010179:Pancreas D050050:Protein Carbonylation D051381:Rats D017382:Reactive Oxygen Species D013482:Superoxide Dismutase D013662:Tea D014280:Triglycerides
Black tea Black tea Oxidative stress BT ALX glutathione 3,3′-diaminobenzidine tetrahydrochloride ALP ALT Antioxidant machineries BUN CAT DAB Diabetes mellitus GPx GR GSH GSSG GST Kombucha tea MDA ROS SOD TBARS TC TG alanine transaminase alkaline phosphatase alloxan monohydrate black tea blood urea nitrogen catalase glutathione disulfide glutathione peroxidase glutathione reductase glutathione-S-transferase malonaldehyde reactive oxygen species superoxide dismutase thiobarbituric acid reactive substance total cholesterol triglyceride