Preventing Surgical Site Infections Using a Natural, Biodegradable, Antibacterial Coating on Surgical Sutures.
Surgical site infections (SSIs) are one of the most common nosocomial infections, which can result in serious complications after surgical interventions. Foreign materials such as implants or surgical sutures are optimal surfaces for the adherence of bacteria and subsequent colonization and biofilm formation. Due to a significant increase in antibiotic-resistant bacterial strains, naturally occurring agents exhibiting antibacterial properties have great potential in prophylactic therapies. The aim of this study was to develop a coating for surgical sutures consisting of the antibacterial substance totarol, a naturally occurring diterpenoid isolated from Podocarpustotara in combination with poly(lactide-co-glycolide acid) (PLGA) as a biodegradable drug delivery system. Hence, non-absorbable monofilament and multifilament sutures were coated with solutions containing different amounts and ratios of totarol and PLGA, resulting in a smooth, crystalline coating. Using an agar diffusion test (ADT), it became evident that the PLGA/totarol-coated sutures inhibited the growth of Staphylococcus aureus over a period of 15 days. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the coated sutures were not cytotoxic to murine fibroblasts. Overall, the data indicates that our innovative, biodegradable suture coating has the potential to reduce the risk of SSIs and postoperative biofilm-formation on suture material without adverse effects on tissue.
['Reinbold|Jochen|J|', 'Uhde|Ann-Kristin|AK|', 'Müller|Ingrid|I|', 'Weindl|Tobias|T|', 'Geis-Gerstorfer|Jürgen|J|', 'Schlensak|Christian|C|', 'Wendel|Hans-Peter|HP|', 'Krajewski|Stefanie|S|']
D045784:Abietanes D000818:Animals D000900:Anti-Bacterial Agents D001422:Bacterial Adhesion D018441:Biofilms D002470:Cell Survival D020099:Coated Materials, Biocompatible D004224:Diterpenes D004337:Drug Carriers D065546:Drug Liberation D005347:Fibroblasts D051379:Mice D008855:Microscopy, Electron, Scanning D000077182:Polylactic Acid-Polyglycolic Acid Copolymer D013211:Staphylococcus aureus D013499:Surface Properties D013530:Surgical Wound Infection D013537:Sutures
surgical site infection biodegradable antibacterial coating suture totarol Staphylococcus aureus