Effect of rapid cryopreservation on meiotic recombination in human spermatocytes.
OBJECTIVE
To evaluate the safety of rapid cryopreservation for human testicular tissues by comparing the meiotic recombination in the fresh and thawed testis after rapid freezing.


METHODS
Twelve male patients with prostate cancer (PC) who had given birth to healthy children at youth and need to receive surgical removal of testicular tissue at present were selected in this study. Testicular tissues from 4 cases of PC patients were randomly divided into two parts, one part as fresh tissue and the other to receive rapid freezing treatment. Fidelity analysis for homologous genetic recombination and synapsis were performed by immunofluorescence after prepared by a micro-spreading technique.


RESULTS
The average number of MLH1 foci per cell, autosomal synaptonemal complex (SC) containing 0∼5 MLH1 foci and percent of cells with one MLH1 focus on XY chromosome showed no difference between the fresh and frozen thawed testicular tissues from the same case (P >0.05). And, no significant difference in the frequency of gaps and splits on SCs was observed in fresh and thawed spermatocytes (P > 0.05).


CONCLUSION
Rapid cryopreservation showed little effect on the frequency of meiotic recombination and fidelity of synapsis in human spermatocytes from PC patients, and acted as an effective method for preserving male fertility.
['Song|Wenyan|W|', 'Zhao|Wanli|W|', 'Yang|Qinglin|Q|', 'Wang|Xuegai|X|', 'Jin|Haixia|H|', 'Yao|Guidong|G|', 'Peng|Zhaofeng|Z|', 'Shi|Senlin|S|', 'Yang|Hongyi|H|', 'Sun|Yingpu|Y|']
D015925:Cryopreservation D006801:Humans D008297:Male D008540:Meiosis D008856:Microscopy, Fluorescence D011467:Prostate D011471:Prostatic Neoplasms D011995:Recombination, Genetic D013090:Spermatocytes D013573:Synaptonemal Complex D013737:Testis
cryopreservation genetic recombination synaptonemal complex meiosis