Effect of Erythrodiol, A Natural Pentacyclic Triterpene from Olive Oil, on the Lipid Membrane Properties.
The effect of erythrodiol, a natural pentacyclic triterpene to which humans are exposed through nutrients, on the lipid membranes is studied using liposomes as a membrane model. Empty and erythrodiol-loaded liposomes were prepared by the reverse phase evaporation method followed by the extrusion and by the thin film hydration method. Liposomes were characterized in terms of size and zeta potential and were imaged by transmission electron microscopy (TEM) and atomic force microscopy (AFM). The effect of erythrodiol on thermotropic behavior of DPPC bilayers is also examined by differential scanning calorimetry (DSC). The DSC thermograms suggested that erythrodiol interacted with the polar head groups of phospholipids and may produce a disruption of the ordering of the alkyl chains. The diffraction light scattering analysis showed that erythrodiol-loaded liposomes presented a decrease in the vesicle size when compared to blank liposomes. Images obtained by TEM confirmed the formation of unilamellar and spherical liposomes. AFM images showed spherical vesicles and single lipid bilayers. The latter were more abundant in the preparations containing erythrodiol than in the blank ones. Moreover, erythrodiol-loaded liposomes tended to rupture into single lipid bilayers during scanning. The study may provide a better understanding of pentacyclic triterpenes-membrane interaction.
['Habib|Lamice|L|', 'Jraij|Alia|A|', 'Khreich|Nathalie|N|', 'Charcosset|Catherine|C|', 'Greige-Gerges|Hélène|H|']
D056945:Hep G2 Cells D006801:Humans D008051:Lipid Bilayers D008081:Liposomes D018625:Microscopy, Atomic Force D046529:Microscopy, Electron, Transmission D015394:Molecular Structure D009828:Oleanolic Acid D000069463:Olive Oil D010316:Particle Size D053978:Pentacyclic Triterpenes D013816:Thermodynamics
Erythrodiol Liposomes Transmission electron microscopy Atomic force microscopy Differential scanning calorimetry Dynamic light scattering