IGF2BP2 Overexpression Indicates Poor Survival in Patients with Acute Myelocytic Leukemia.
BACKGROUND/AIMS
IGF2BP2 has been reported to serve as an oncogene in various solid cancers. However, the role of IGF2BP2 in acute myelocytic leukemia (AML) is still unknown.


METHODS
Public databases Gene Omnibus was used to evaluate the expression of IGF2BP2 in AML patients and healthy controls. In addition, primary cells from these two populations were prepared by Ficoll density centrifugation. Rt-qPCR and western blot were used to detect IGF2BP2 expression in the primary cells from these two populations. Meta-analysis was performed to evaluate the association of IGF2BP2 and prognosis. Lentivirus-based shRNAs were used to knock down IGF2BP2 in AML cell lines KG-1a and Kasumi.


RESULTS
We searched the public database Gene Omnibus and analyzed IGF2BP2 expression in both AML and healthy populations. The results showed that IGF2BP2 was overexpressed in AML patients. To verify this phenomenon in fresh human samples, we compared the expression of IGF2BP2 in primary cells from 10 AML patients and 10 healthy controls and found that the expression of IGF2BP2 was upregulated in AML primary cells. More importantly, we observed that IGF2BP2 expression was negatively correlated with the CEBPA mutation status, which is an indicator of good prognosis (RR=0.648, p=0.0001). In addition, IGF2BP2 expression was positively associated with poor prognostic factors, such as the FLT3-ITD mutation (RR=1.198, p=0.0009) and IDH1 mutation (RR=1.354, p=0.0003), as well as intermediate and poor cytogenetic risk (RR=1.214, p=0.0026). To evaluate the prognostic value of IGF2BP2 in AML, we further performed a meta-analysis of 8 studies consisting of 1731 patients and found that IGF2BP2 overexpression was correlated with worse overall survival in AML patients [HR=1.31(1.16-1.49); p = 0.00]. Furthermore, we performed Gene Omnibus and Gene Set Enrichment analyses and found that the genes regulated by IGF2BP2 were mainly enriched in cell proliferation. IGF2BP2 knockdown by 4 different shRNA vectors significantly inhibited the growth of two AML cell lines, KG-1a and Kasumi.


CONCLUSION
Thus, IGF2BP2 may serve as a biomarker to predict the prognosis of AML and as a potential target in AML.
['He|Xin|X|', 'Li|Wenlu|W|', 'Liang|Xiaogong|X|', 'Zhu|Xuan|X|', 'Zhang|Lei|L|', 'Huang|Yu|Y|', 'Yu|Teng|T|', 'Li|Shu|S|', 'Chen|Zhigang|Z|']
D049109:Cell Proliferation D005260:Female D015973:Gene Expression Regulation, Leukemic D006801:Humans D015470:Leukemia, Myeloid, Acute D008297:Male D008875:Middle Aged D011379:Prognosis D016601:RNA-Binding Proteins D016019:Survival Analysis D014407:Tumor Cells, Cultured D015854:Up-Regulation
IGF2BP2 Acute myelocytic leukemia Prognosis Proliferation