MicroRNA-361-5p inhibits papillary thyroid carcinoma progression by targeting ROCK1.
MicroRNAs function as key regulators in various human cancers, including papillary thyroid cancer (PTC). MiR-361-5p has been proved to be a tumor suppressor in multiple cancers. However, the function of miR-361-5p in PTC remains unknown. In this study, we aimed to determine the function of miR-361-5p in PTC progression, and elaborate the mechanism by which miR-361-5p acts in PTC. Here, we report that miR-361-5p expression levels were significantly downregulated in PTC tissues and cell lines, as detected by reverse transcription-quantitative polymerase chain reaction (qRT-PCR) analysis. Functional analysis revealed that overexpression of miR-361-5p significantly inhibited cell proliferation, migration, invasion in vitro, as well as suppressed tumor growth in vivo. Bioinformatic analysis showed that Rho-associated coiled-coil kinase 1 (ROCK1) was a predicted target of miR-361-5p, which was further validated by the dual-luciferase reporter assay, qRT-PCR, and western blot analysis. In addition, an inverse expression pattern was also observed between miR-361-5p and ROCK1 in a cohort of PTC tissues. Rescue experiments showed that restoration of ROCK1 expression significantly reversed the suppressive effect of miR-361-5p on cell proliferation, migration, and invasion in PTC cells. Taken together, these findings suggest that miR-361-5p is a novel potential therapeutic target for thyroid cancer.
['Li|Rui|R|', 'Dong|Bingfei|B|', 'Wang|Zhengmin|Z|', 'Jiang|Tao|T|', 'Chen|Guang|G|']
D000818:Animals D001483:Base Sequence D002291:Carcinoma, Papillary D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D018450:Disease Progression D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008297:Male D008807:Mice, Inbred BALB C D008819:Mice, Nude D035683:MicroRNAs D008875:Middle Aged D009361:Neoplasm Invasiveness D000077273:Thyroid Cancer, Papillary D013964:Thyroid Neoplasms D047368:Tumor Burden D014410:Tumor Stem Cell Assay D054460:rho-Associated Kinases
Papillary thyroid carcinoma ROCK1 Thyroid cancer miR-361-5p Key words