Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells.
In the present study, we evaluated for the first time the photoprotective effect of fish bone bioactive peptides (FBBP) preparation isolated from silver carp (Hypophthalmichthys molitrix) discarded tissue using in vitro experimental models of skin cells exposed to ultraviolet B (UVB) irradiation and stressing agents. FBBP preparation was obtained by papain treatment of minced bones and centrifugal ultrafiltration, and the molecular weight (MW) distribution was characterized by size exclusion and reversed-phase high performance liquid chromatography (RP-HPLC). In vitro assessment of the effect of FBBP pretreatment in UVB-irradiated L929 fibroblasts and HaCaT keratinocytes revealed their cytoprotective activity. Their capacity to efficiently reduce reactive oxygen species (ROS) production and lipid peroxidation varied in a dose-dependent manner, and it was greater in fibroblasts. A decrease of proinflammatory cytokines secretion, in particular of tumor necrosis factor alpha (TNF-α), was found after FBBP pretreatment of THP-1-derived inflamed macrophages. Melanin production and tyrosinase activity investigated in UVB-irradiated Mel-Juso cells were lowered in direct relation to FBBP concentrations. FBBP fractions with high radical scavenging activity were separated by ion exchange chromatography, and two collagenic sequences were identified. All these results offer new scientific data on aquaculture fish bone-derived peptides confirming their ability to control the antioxidant, anti-inflammatory and pigmentation processes developed during UV irradiation of skin cells and recommend their use as valuable natural ingredients of photoprotective cosmeceutical products.
['Iosageanu|Andreea|A|0000-0002-1137-0352', 'Ilie|Daniela|D|', 'Craciunescu|Oana|O|0000-0003-0625-3650', 'Seciu-Grama|Ana-Maria|AM|', 'Oancea|Anca|A|', 'Zarnescu|Otilia|O|', 'Moraru|Ionut|I|0000-0003-2902-1537', 'Oancea|Florin|F|0000-0002-7723-1814']
D000818:Animals D000975:Antioxidants D001842:Bone and Bones D002470:Cell Survival D016207:Cytokines D019610:Cytoprotection D005399:Fishes D000084282:HaCaT Cells D006801:Humans D007249:Inflammation D018836:Inflammation Mediators D042541:Intracellular Space D008264:Macrophages D008315:Malondialdehyde D008543:Melanins D051379:Mice D008970:Molecular Weight D010084:Oxidation-Reduction D010455:Peptides D010858:Pigmentation D017382:Reactive Oxygen Species D012867:Skin D013056:Spectrophotometry, Ultraviolet D000074084:THP-1 Cells D014466:Ultraviolet Rays
lipid peroxidation proinflammatory cytokines antioxidant activity biological activity collagen peptides free radicals scavenging melanogenesis photoaging sunscreen