Studies of proliferation and chondrogenic differentiation of rat adipose stem cells using an anti-oxidative polyurethane scaffold combined with cyclic compression culture.
The adipose stem cell is a potential candidate for the autologous chondrocytes repairing approach because of the abundance of fat in the animal body and its versatile differentiation capability. In this study, rat adipose stem cells (rASCs) were seeded into anti-oxidative N-acetylcysteine (NAC) grafted polyurethane (PU) scaffold and then combined with short dynamic compressive stimulation (24 h) to induce rASCs chondrogenesis differentiation in vitro. The inner pore surface of the PU scaffold was first modified via alginate and type I collagen to promote rASCs adherence. The modified layers crosslinked by genipin showed outstanding stability after ultrasonic treatment, indicating the modified layers were stable and can keep the cells adhesion well during dynamic compressive stimulation. After inner pore surface modification and 10 mM NAC grafting, the PU scaffold-A-C-G (graft 10 mM NAC) has shown the best proliferation efficiency with homogeneous cell distribution after 72hr static culture. After short term dynamic compressive stimulation, significant gene expression in chondrogenic markers, Sox-9, and Aggrecan, were noted in both PU scaffold-A-C-G and PU scaffold-A-C-G (graft 10 mM NAC). Considering the cell proliferation efficiency and gene expression, the anti-oxidative NAC grafted PU scaffold combined with short term dynamic compressive stimulation could be useful for cell culturing in stem cell therapy.
['Tseng|Shen-Jui|SJ|', 'Huang|Shih-Ting|ST|', 'Wu|Chia-Ching|CC|', 'Cheng|Chi-Hui|CH|', 'Lin|Jui-Che|JC|']
D000111:Acetylcysteine D000273:Adipose Tissue D053674:Aggrecans D000818:Animals D001672:Biocompatible Materials D002448:Cell Adhesion D018929:Cell Culture Techniques D002454:Cell Differentiation D002460:Cell Line D049109:Cell Proliferation D019902:Chondrocytes D020219:Chondrogenesis D015870:Gene Expression D039823:Iridoids D011140:Polyurethanes D051381:Rats D055758:SOX9 Transcription Factor D013234:Stem Cells
Chondrogenic differentiation Rat adipose stem cell N-acetylcysteine Dynamic compressive stimulation Surface modification