Combination of glycosphingosomes and liposomal doxorubicin shows increased activity against dimethyl-α-benzanthracene-induced fibrosarcoma in mice.
The present study aimed to assess the antitumor effect of glycosphingolipid-incorporated liposomes (glycosphingosomes) in combination with liposomal doxorubicin (Lip-Dox) in a mouse model of fibrosarcoma. Glycosphingosomes were prepared by incorporating glycosphingolipids isolated from Sphingomonas paucimobilis into the liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, cholesterol, and cardiolipin. Tumors were induced by administering dimethyl-α-benzanthracene, and tumor-bearing mice were treated with various formulations of Dox, including free Dox, Lip-Dox, or glycosphingosomes + Lip-Dox. Mice were observed for 90 days to monitor their survival and tumor size. Free Dox, but not Lip-Dox or a combination of glycosphingosomes and Lip-Dox, caused the substantial depletion of leukocytes and significantly increased the levels of lactate dehydrogenase and creatinine kinase in mice. Tumor-bearing mice treated with a combination of glycosphingosomes and Lip-Dox showed restricted tumor growth and increased survival when compared to those treated with free Dox or Lip-Dox. The results of the present study suggest that a combination of glycosphingosomes and Lip-Dox may prove to be very effective in the treatment of tumors.
['Khan|Masood A|MA|', 'Aljarbou|Ahmed N|AN|', 'Aldebasi|Yousef H|YH|', 'Alorainy|Mohammed S|MS|', 'Khan|Arif|A|']
D015060:1,2-Dipalmitoylphosphatidylcholine D015127:9,10-Dimethyl-1,2-benzanthracene D000818:Animals D049109:Cell Proliferation D002626:Chemistry, Pharmaceutical D004317:Doxorubicin D005260:Female D005354:Fibrosarcoma D051379:Mice D011092:Polyethylene Glycols D016019:Survival Analysis
glycosphingolipids tumors NKT cells chemotherapy