A study of the bactericidal, anti-biofouling, cytotoxic and antioxidant properties of actinobacterially synthesised silver nanoparticles.
Actinobacteria- mediated synthesis of silver nanoparticles (AgNPs) is a reliable, eco-friendly and important aspect of nanobiotechnology. In this study, aqueous silver ions, which were exposed to an actinobacterial biomass of Streptomyces naganishii (MA7), were reduced to form stable AgNPs under optimised conditions. The microbially synthesised AgNPs were characterised by UV-Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), selected area electron diffraction (SAED), energy dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), atomic force microscopy (AFM) and high- resolution transmission electron microscopy (HR-TEM). The size (5-50 nm) and shape (spherical) of the AgNPs were determined. The biosynthesised AgNPs exhibited good bactericidal, anti-biofouling, antioxidant and cytotoxic effects with regards to the HeLa cell line. A single protein band with a molecular weight of 44 kDa was obtained after partial purification of the culture filtrate via polyacrylamide gel electrophoresis. The potent actinobacterial strain was identified by its molecular (16s rRNA sequencing), phenotypic and cultural characteristics. The current study demonstrated the potential use of the extremophilic actinobacterial strain of S. naganishii (MA7) as a novel source for AgNPs synthesis with improved biomedical applications.
['Shanmugasundaram|Thangavel|T|', 'Radhakrishnan|Manikkam|M|', 'Gopikrishnan|Venugopal|V|', 'Pazhanimurugan|Raasaiyah|R|', 'Balagurunathan|Ramasamy|R|']
D000900:Anti-Bacterial Agents D000975:Antioxidants D018441:Biofilms D057219:Biofouling D016923:Cell Death D006367:HeLa Cells D006801:Humans D053768:Metal Nanoparticles D008826:Microbial Sensitivity Tests D012834:Silver D013056:Spectrophotometry, Ultraviolet D017550:Spectroscopy, Fourier Transform Infrared D013302:Streptomyces
Cytotoxicity Silver nanoparticle Streptomyces naganishii AFM Antibacterial