LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.
Keloid is a skin disease characterized by fibrous hyperplasia, which is often difficult to cure. Long non-coding RNAs (lncRNAs) have been shown to be associated with the development of many diseases. However, the role and mechanism of lncRNA H19 in keloid has been less studied. Our study found that lncRNA H19 expression was increased in keloid tissues and fibroblasts. Besides, H19 knockdown hindered the proliferation, migration, invasion, extracellular matrix (ECM) deposition, and enhanced the apoptosis of keloid fibroblasts. Further experiments showed that microRNA (miR)-769-5p could be sponged by H19, and its knockdown reversed the suppression effect of H19 knockdown on keloid formation. Eukaryotic initiation factor 3A (EIF3A) was found to be a target of miR-769-5p, and its overexpression inverted the inhibition effect of miR-769-5p overexpression on keloid formation. Moreover, the expression of EIF3A was regulated by H19 and miR-769-5p in keloid fibroblasts. Collectively, LncRNA H19 might play an active role in keloid formation, which might provide a new target for the treatment of keloid.
['Xu|Lingang|L|', 'Sun|Nan|N|', 'Li|Guangshuai|G|', 'Liu|Linbo|L|http://orcid.org/0000-0001-6924-9099']
D017209:Apoptosis D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D039621:Eukaryotic Initiation Factor-3 D005109:Extracellular Matrix D005347:Fibroblasts D020869:Gene Expression Profiling D015972:Gene Expression Regulation, Neoplastic D020868:Gene Silencing D006801:Humans D007627:Keloid D035683:MicroRNAs D009361:Neoplasm Invasiveness D062085:RNA, Long Noncoding D034741:RNA, Small Interfering
H19 Keloid miR-769-5p EIF3A Fibroblasts