Genotoxicity and activation of cellular defenses in transplanted zebra mussels Dreissena polymorpha along the Seine river.
The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the (32)P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature. Increase of DNA adducts after two months of transplantation could be correlated with strong modulation of gene expression implicated in detoxification processes. Indeed, PgP and HSP70 gene expressions were similarly induced in gills and digestive glands while SOD and CAT expressions were down regulated in both tissues. AHR, GST and MT genes were differently regulated depending upon the tissue studied and the level of contamination in the different sites. We demonstrated that mussels transplanted in the different stations with pollution gradient were able to biotransform PAHs, assessed by DNA adduct formation and the high decrease of detoxification genes. Specific DNA adducts pattern obtained after one and two month mussel transplantations demonstrated the relevance of DNA adduct as biomarker of environmental pollution.
['Châtel|Amélie|A|', 'Faucet-Marquis|Virginie|V|', 'Gourlay-Francé|Catherine|C|', 'Pfohl-Leszkowicz|Annie|A|', 'Vincent-Hubert|Françoise|F|']
D000818:Animals D015415:Biomarkers D002374:Catalase D018736:DNA Adducts D004249:DNA Damage D049875:Dreissena D015870:Gene Expression D005880:Gills D005982:Glutathione Transferase D011084:Polycyclic Aromatic Hydrocarbons D018336:Receptors, Aryl Hydrocarbon D045483:Rivers D013482:Superoxide Dismutase D014874:Water Pollutants, Chemical
Dreissena polymorpha DNA adducts Pollution Detoxification RT-PCR Biomarker