Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin.
OBJECTIVE
The current study aimed to elucidate the effect of vanillin on behavioral changes, oxidative stress, and histopathological changes induced by potassium bromate (KBrO3), an environmental pollutant, in the cerebellum of adult mice.


METHODS
The animals were divided into four groups: group 1 served as a control, group 2 received KBrO3, group 3 received KBrO3 and vanillin, and group 4 received only vanillin. We then measured behavioral changes, oxidative stress, and molecular and histological changes in the cerebellum.


RESULTS
We observed significant behavioral changes in KBrO3-exposed mice. When investigating redox homeostasis in the cerebellum, we found that mice treated with KBrO3 had increased lipid peroxidation and protein oxidation in the cerebellum. These effects were accompanied by decreased Na+-K+ and Mg2+ ATPase activity and antioxidant enzyme gene expression when compared to the control group. Additionally, there was a significant increase in cytokine gene expression in KBrO3-treated mice. Microscopy revealed that KBrO3 intoxication resulted in numerous degenerative changes in the cerebellum that were substantially ameliorated by vanillin supplementation. Co-administration of vanillin blocked the biochemical and molecular anomalies induced by KBrO3.


CONCLUSION
Our results demonstrate that vanillin is a potential therapeutic agent for oxidative stress associated with neurodegenerative diseases.
['Ben Saad|Hajer|H|', 'Driss|Dorra|D|', 'Jaballi|Imen|I|', 'Ghozzi|Hanen|H|', 'Boudawara|Ons|O|', 'Droguet|Michael|M|', 'Magné|Christian|C|', 'Nasri|Monsef|M|', 'Zeghal|Khaled Mounir|KM|', 'Hakim|Ahmed|A|', 'Ben Amara|Ibtissem|I|']
D000818:Animals D000975:Antioxidants D001522:Behavior, Animal D001547:Benzaldehydes D001959:Bromates D002531:Cerebellum D016207:Cytokines D004785:Environmental Pollutants D015870:Gene Expression D015227:Lipid Peroxidation D051379:Mice D018384:Oxidative Stress D045442:Rotarod Performance Test
Cerebellum Vanillin Behavior Potassium bromate ATPases Genes expression