Multifunctional CuO nanoparticles with cytotoxic effects on KYSE30 esophageal cancer cells, antimicrobial and heavy metal sensing activities.
In this work, fluorescent copper oxide nanoparticles (CuO NPs) were green synthesized using viable cells, cell lysate supernatant (CLS) and protein extracts of luminescent Vibrio sp. VLC. Biogenic CuO NPs were then characterized by XRD, FTIR, UV/Vis spectroscopy, TEM, DLS, and PL spectroscopy. Results showed that CLS method was more efficient for CuO NPs production, therefore CuO NPs synthesized by this method from copper sulfate (CuO NPs-1) and/or copper nitrate (CuO NPs-2) were used for further studies. The crystallite size of polydispersed CuO NPs-1 and CuO NPs-2 were about 8.83 and 8.77 nm, respectively indicating their suitability for biological applications. Antibacterial activity of CuO NPs was determined using broth microdilution, well diffusion agar, and time-kill curves methods. Both CuO NP-1 and CuO NP-2 inhibited bacterial growth at the minimum inhibitory concentration (MIC) of 625 mg/L except St. mutants (MIC = 1250 mg/L). Emission of fluorescent light from the surface of NPs was increased when exposed to Cd2+, As2+ and Hg2+ ions but decreased by Pb2+ ions. Results showed that CuO NP-1 had anticancer properties against KYSE30 esophageal cancer cell line (IC50 = 13.96 mg/L) while no higher cytotoxic effects were observed on Human Dermal Fibroblasts (HDF) (IC50 = 48.88 mg/L).
['Nakhaeepour|Zahra|Z|', 'Mashreghi|Mansour|M|', 'Matin|Maryam M|MM|', 'NakhaeiPour|Ali|A|', 'Housaindokht|Mohammad Reza|MR|']
D000900:Anti-Bacterial Agents D000970:Antineoplastic Agents D001419:Bacteria D001424:Bacterial Infections D045744:Cell Line, Tumor D003300:Copper D004938:Esophageal Neoplasms D005456:Fluorescent Dyes D055772:Green Chemistry Technology D006801:Humans D019216:Metals, Heavy D053758:Nanoparticles D013050:Spectrometry, Fluorescence D014733:Vibrio
Anticancer properties Antimicrobial activity Fluorescent CuO KYSE30 cell line Multifunctional biogenic NPs