Induction of herpes simplex virus type 1 cell-to-cell spread inhibiting antibodies by a calcium phosphate nanoparticle-based vaccine.
Herpes simplex viruses 1 and 2 are among the most ubiquitous human infections and persist lifelong in their host. Upon primary infection or reactivation from ganglia, the viruses spread by direct cell-cell contacts (cell-to-cell spread) and thus escape from the host immune response. We have developed a monoclonal antibody (mAb 2c), which inhibits the HSV cell-to-cell spread, thereby protecting from lethal genital infection and blindness in animal models. In the present study we have designed a nanoparticle-based vaccine to induce protective antibody responses exceeding the cell-to-cell spread inhibiting properties of mAb 2c. We used biodegradable calcium phosphate (CaP) nanoparticles coated with a synthetic peptide that represents the conformational epitope on HSV-1 gB recognized by mAb 2c. The CaP nanoparticles additionally contained a TLR-ligand CpGm and were formulated with adjuvants to facilitate the humoral immune response. This vaccine effectively protected mice from lethal HSV-1 infection by inducing cell-to-cell spread inhibiting antibodies.
['Kopp|Mathis|M|', 'Aufderhorst|Ulrich Wilhelm|UW|', 'Alt|Mira|M|', 'Dittmer|Ulf|U|', 'Eis-Hübinger|Anna-Maria|AM|', 'Giebel|Bernd|B|', 'Roggendorf|Michael|M|', 'Epple|Matthias|M|', 'Krawczyk|Adalbert|A|']
D000818:Animals D000911:Antibodies, Monoclonal D000914:Antibodies, Viral D002130:Calcium Phosphates D002522:Chlorocebus aethiops D005260:Female D018259:Herpesvirus 1, Human D022283:Herpesvirus Vaccines D051379:Mice D008807:Mice, Inbred BALB C D053758:Nanoparticles D014709:Vero Cells
Herpes simplex virus Cell-to-cell spread Antibodies CaP nanoparticles