β-asarone and levodopa coadministration increases striatal levels of dopamine and levodopa and improves behavioral competence in Parkinson's rat by enhancing dopa decarboxylase activity.
Levodopa (L-dopa) is the key component in Parkinson's disease (PD) treatment. Recently, we demonstrated that β-asarone improves the motor behavior of rats with unilateral striatal 6-hydroxydopamine lesion. Striatal level of dopamine (DA) and L-dopa increased after β-asarone and L-dopa co-administered treatment in healthy rat. Since its effects and mechanisms on PD rats are still unclear, we investigated whether coadministration could help treat PD rats. Here, PD rats were randomly divided into seven groups (n=10/group): an untreated group, a Madopar-treated group, a L-dopa-treated group, a β-asarone-treated group, and groups receiving low, medium or high doses of β-asarone respectively plus the same dose of L-dopa. The sham-operated group rats were injected with saline. Treatments were administered to the rats twice per day continuously for 30days. The behavioral tests were assessed. Neurotransmitters, dopa decarboxylase (DDC), tyrosine hydroxylase (TH), catechol-O-methyltransferase (COMT), monoamine oxidase B (MAO-B) and dopamine transporter (DAT) levels were detected. The pathological characteristics of liver and kidney and ultrastructure of dopaminergic neurons were observed. The behavior of PD rats improved significantly after co-administered treatment compared with the untreated group. In addition, our results also showed that co-administered treatment increased L-dopa, DA, DOPAC, HVA and 5-HT levels, enhanced the MAO-B, COMT, TH and DAT levels, reduced creatinine level, decreased the amount of lysosome and mitochondria and showed no liver and kidney toxicity. These findings suggest that co-administered treatment could elevate striatal levels of L-dopa and DA and improve the behavioral abilities in PD rats by regulating the DDC, TH, MAO-B, COMT and DAT levels.
['Huang|Liping|L|', 'Deng|Minzhen|M|', 'Zhang|Sheng|S|', 'Lu|Shiyao|S|', 'Gui|Xuehong|X|', 'Fang|Yongqi|Y|']
D000081248:Allylbenzene Derivatives D000818:Animals D000840:Anisoles D000978:Antiparkinson Agents D001522:Behavior, Animal D001545:Benserazide D002394:Catechol O-Methyltransferase D003342:Corpus Striatum D004296:Dopa Decarboxylase D004298:Dopamine D050483:Dopamine Plasma Membrane Transport Proteins D059290:Dopaminergic Neurons D004338:Drug Combinations D004359:Drug Therapy, Combination D005260:Female D007980:Levodopa D008297:Male D016627:Oxidopamine D020734:Parkinsonian Disorders D051381:Rats D017207:Rats, Sprague-Dawley
β-asarone Dopa decarboxylase Behavioral tests Neurotransmitters Dopaminergic neurons Parkinson’s disease