Serological Profiling for Malaria Surveillance Using a Standard ELISA Protocol.
The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost method for measuring soluble ligands such as antibodies and proteins in biological samples. For analysis of specific antibodies in serum, a capture antigen is immobilized onto a solid polystyrene surface from which it can capture the antibodies. The captured antibodies are subsequently detected using a secondary antibody conjugated to an enzyme. Detection is accomplished by addition of a colorimetric substrate, and the readout is absorbance (optical density). Here, we provide a detailed standardized ELISA protocol for the quantification of antibodies against malaria antigens.
['Murungi|Linda M|LM|', 'Kimathi|Rinter K|RK|', 'Tuju|James|J|', 'Kamuyu|Gathoni|G|', 'Osier|Faith H A|FHA|']
D000906:Antibodies D000941:Antigens D000962:Antimalarials D004797:Enzyme-Linked Immunosorbent Assay D006801:Humans D008288:Malaria
ELISA Antibodies Serum Antigens Optical density