Impedimetric PSA aptasensor based on the use of a glassy carbon electrode modified with titanium oxide nanoparticles and silk fibroin nanofibers.
This article describes an impedimetric aptasensor for the prostate specific antigen (PSA), a widely accepted prostate cancer biomarker. A glassy carbon electrode (GCE) was modified with titanium oxide nanoparticles (TiO2) and silk fibroin nanofiber (SF) composite. The aptasensor was obtained by immobilizing a PSA-binding aptamer on the AuNP-modified with 6-mercapto-1-hexanol. The single fabrication steps were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The assay has two linear response ranges (from 2.5 fg.mL-1 to 25 pg.mL-1, and from 25 pg.mL-1 to 25 ng.mL-1) and a 0.8 fg.mL -1 detection limit. After optimization of experimental conditions, the sensor is highly selective for PSA over bovine serum albumin and lysozyme. It was successfully applied to the detection of PSA in spiked serum samples. Graphical abstract Schematic of the fabrication of an aptasensor for the prostate specific antigen (PSA). It is based on the use of a glassy carbon electrode modified with gold nanoparticles and titanium oxide-silk fibroin. The immobilization process of aptamer and interaction with PSA were followed by electrochemical impedance spectroscopy technique.
['Benvidi|Ali|A|', 'Banaei|Maryam|M|', 'Tezerjani|Marzieh Dehghan|MD|', 'Molahosseini|Hosein|H|', 'Jahanbani|Shahriar|S|']
D052157:Aptamers, Nucleotide D055664:Electrochemical Techniques D004566:Electrodes D005349:Fibroins D006801:Humans D008297:Male D053768:Metal Nanoparticles D015335:Molecular Probes D057139:Nanofibers D017430:Prostate-Specific Antigen D011471:Prostatic Neoplasms D013057:Spectrum Analysis D014025:Titanium
Aptasensor Titanium oxide nanoparticles Silk fibroin nanofibers Composite Immobilization Hexacyanoferrate Label-free detection Prostate-specific antigen (PSA)