The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M.
MicroRNAs (miRNAs) in the AGO-containing RISC complex control messenger RNA (mRNA) translation by binding to mRNA 3' untranslated region (3'UTR). The relationship between miRNAs and other regulatory factors that also bind to mRNA 3'UTR, such as CPEB1 (cytoplasmic polyadenylation element-binding protein), remains elusive. We found that both CPEB1 and miR-15b control the expression of WEE1, a key mammalian cell cycle regulator. Together, they repress WEE1 protein expression during G1 and S-phase. Interestingly, the 2 factors lose their inhibitory activity at the G2/M transition, at the time of the cell cycle when WEE1 expression is maximal, and, moreover, rather activate WEE1 translation in a synergistic manner. Our data show that translational regulation by RISC and CPEB1 is essential in cell cycle control and, most importantly, is coordinated, and can be switched from inhibition to activation during the cell cycle.
['Kratassiouk|Gueorgui|G|', 'Pritchard|Linda L|LL|', 'Cuvellier|Sylvain|S|', 'Vislovukh|Andrii|A|', 'Meng|Qingwei|Q|', 'Groisman|Regina|R|', 'Degerny|Cindy|C|', 'Deforzh|Evgeny|E|', 'Harel-Bellan|Annick|A|', 'Groisman|Irina|I|']
D001483:Base Sequence D018797:Cell Cycle Proteins D059565:G2 Phase Cell Cycle Checkpoints D006367:HeLa Cells D006801:Humans D035683:MicroRNAs D009687:Nuclear Proteins D011505:Protein-Tyrosine Kinases D034622:RNA Interference D014157:Transcription Factors D039221:mRNA Cleavage and Polyadenylation Factors
WEE1 CPEB1 miR-15b cytoplasmic polyadenylation cell cycle translational regulation