Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-small Cell Lung Cancer Cells.
Autophagy is increasingly identified as a central player in many cellular activities from cell proliferation to cell division, migration, and differentiation. However, it is also considered as a double-edged sword in cancer biology which either promotes oncogenesis/invasion or sensitizes the tumor cells to chemotherapy induced apoptosis. Recent investigations have provided direct evidence for regulation of cellular phenotype via autophagy pathway. One of the most important types of phenotype conversion is Epithelial-Mesenchymal-Transition (EMT), resulting in alteration of epithelial cell properties to a more mesenchymal form. In the current chapter, we provide a method which is established and being used in our laboratory for detection of autophagy and EMT in lung epithelial cells and show the involvement of autophagy in modulation of cellular phenotype.
['Alizadeh|Javad|J|', 'Shojaei|Shahla|S|', 'Sepanjnia|Adel|A|', 'Hashemi|Mohammad|M|', 'Eftekharpour|Eftekhar|E|', 'Ghavami|Saeid|S|']
D000072283:A549 Cells D001343:Autophagy D000071183:Autophagy-Related Proteins D002289:Carcinoma, Non-Small-Cell Lung D002454:Cell Differentiation D045744:Cell Line, Tumor D049109:Cell Proliferation D002470:Cell Survival D058750:Epithelial-Mesenchymal Transition D006801:Humans D008175:Lung Neoplasms D010641:Phenotype D015398:Signal Transduction
Autophagy Epithelial to mesenchymal transition Lung cancer Phenotype conversion