A novel recycling system for nano-magnetic molecular imprinting immobilised cellulases: Synergistic recovery of anthocyanin from fruit and vegetable waste.
Fruit and vegetable waste (FVW) is become a serious problem in developing countries. Enzymolysis is a potentially useful method for the treatment of FVW. In the present study, novel recycled magnetic molecular imprinting immobilised cellulases were prepared based on magnetic modified chitosan (MCTS) and Fe3O4. The properties of obtained were characterised by IR and grain-size measurements. Evaluation of a single factor affecting the loading efficiency of supports and the mixed immobilised enzymes showed better capacity than single immobilised, or free, enzymes. The immobilisation process could improve cellulase stability and repeatability of the method. Meanwhile, the kinetic parameters were also verified. The immobilised enzymes retained most of their capacity after 60days' storage while free enzymes lost it within 30days. Tests showed that the immobilised enzymes developed excellent capacity and five anthocyanins were collected.
['Yuan|Bo|B|', 'Yang|Xù-Qin|XQ|', 'Xue|Ling-Wei|LW|', 'Feng|Yan-Nan|YN|', 'Jiang|Ji-Hong|JH|']
D000872:Anthocyanins D044602:Cellulases D004800:Enzymes, Immobilized D005638:Fruit D005947:Glucose D006863:Hydrogen-Ion Concentration D007700:Kinetics D008280:Magnetics D054802:Molecular Imprinting D053758:Nanoparticles D059027:Recycling D013696:Temperature D013997:Time Factors D014675:Vegetables D014866:Waste Products
Nano-magnetic molecular imprinting Immobilisation Synergistic recovery Fruit and vegetable waste