Effect of butyrate and Lactobacillus GG on a butyrate receptor and transporter during Campylobacter jejuni exposure.
Campylobacter jejuni frequently infects humans causing many gastrointestinal symptoms, fever, fatigue and several long-term debilitating diseases. Current treatment for campylobacteriosis includes rehydration and in some cases, antibiotic therapy. Probiotics are used to treat several gastrointestinal diseases. Butyrate is a short-chain fatty acid known to promote intestinal health. Interaction of butyrate with its respective receptor (HCAR2) and transporter (SLC5A8), both expressed in the intestine, is associated with water and electrolyte absorption as well as providing defense against colon cancer and inflammation. Alterations in gut microbiota influence the presence of HCAR2 and SLC5A8 in the intestine. We hypothesized that adherence and/or invasion of C. jejuni and alterations in HCAR2 and SLC5A8 expression would be minimized with butyrate or Lactobacillus GG (LGG) pretreatment of Caco-2 cells. We found that both C. jejuni adhesion but not invasion was reduced with butyrate pretreatment. While LGG pretreatment did not prevent C. jejuni adhesion, it did result in reduced invasion which was associated with altered cell supernate pH. Both butyrate and LGG protected HCAR2 and SLC5A8 protein expression following C. jejuni infection. These results suggest that the first stages of C. jejuni infection of Caco-2 cells may be minimized by LGG and butyrate pretreatment.
['Cresci|Gail A M|GAM|', 'Mayor|Paul C|PC|', 'Thompson|Stuart A|SA|']
D001422:Bacterial Adhesion D002087:Butyrates D018938:Caco-2 Cells D002169:Campylobacter Infections D016123:Campylobacter jejuni D002352:Carrier Proteins D002478:Cells, Cultured D015870:Gene Expression D006801:Humans D006863:Hydrogen-Ion Concentration D052201:Lactobacillus rhamnosus D056265:Microbial Interactions D027501:Monocarboxylic Acid Transporters D043562:Receptors, G-Protein-Coupled D011978:Receptors, Nicotinic
butyrate Lactobacillus GG Campylobacter jejuni Caco-2