Low level laser therapy reduces acute lung inflammation in a model of pulmonary and extrapulmonary LPS-induced ARDS.
The present study aimed to investigate the effects low level laser therapy (LLLT) in a LPS-induced pulmonary and extrapulmonary acute respiratory distress syndrome (ARDS) in BALB/c mice. Laser (830nm laser, 9J/cm(2), 35mW, 80s per point, 3 points per application) was applied in direct contact with skin, 1h after LPS administration. Mice were distributed in control (n=6; PBS), ARDS IT (n=7; LPS orotracheally 10μg/mouse), ARDS IP (n=7; LPS intra-peritoneally 100μg/mouse), ARDS IT+Laser (n=9; LPS intra-tracheally 10μg/mouse), ARDS IP+Laser (n=9; LPS intra-peritoneally 100μg/mouse). Twenty-four hours after last LPS administration, mice were studied for pulmonary inflammation by total and differential cell count in bronchoalveolar lavage (BAL), cytokines (IL-1beta, IL-6, KC and TNF-alpha) levels in BAL fluid and also by quantitative analysis of neutrophils number in the lung parenchyma. LLLT significantly reduced pulmonary and extrapulmonary inflammation in LPS-induced ARDS, as demonstrated by reduced number of total cells (p<0.001) and neutrophils (p<0.001) in BAL, reduced levels of IL-1beta, IL-6, KC and TNF-alpha in BAL fluid and in serum (p<0.001), as well as the number of neutrophils in lung parenchyma (p<0.001). LLLT is effective to reduce pulmonary inflammation in both pulmonary and extrapulmonary model of LPS-induced ARDS.
['Oliveira|Manoel Carneiro|MC|', 'Greiffo|Flávia Regina|FR|', 'Rigonato-Oliveira|Nicole Cristine|NC|', 'Custódio|Ricardo Wesley Alberca|RW|', 'Silva|Vanessa Roza|VR|', 'Damaceno-Rodrigues|Nilsa Regina|NR|', 'Almeida|Francine Maria|FM|', 'Albertini|Regiane|R|', 'Lopes-Martins|Rodrigo Álvaro B|RÁ|', 'de Oliveira|Luis Vicente Franco|LV|', 'de Carvalho|Paulo de Tarso Camillo|Pde T|', 'Ligeiro de Oliveira|Ana Paula|AP|', 'Leal|Ernesto César P|EC|', 'Vieira|Rodolfo P|RP|']
D000208:Acute Disease D000818:Animals D001992:Bronchoalveolar Lavage Fluid D016207:Cytokines D004195:Disease Models, Animal D007249:Inflammation D008070:Lipopolysaccharides D028022:Low-Level Light Therapy D008168:Lung D008297:Male D051379:Mice D008807:Mice, Inbred BALB C D009504:Neutrophils D012128:Respiratory Distress Syndrome
Lung inflammation ARDS LLLT LPS Bronchoalveolar lavage Cytokines