Osteoblast cell membrane chromatography coupled with liquid chromatography and time-of-flight mass spectrometry for screening specific active components from traditional Chinese medicines.
A method using osteoblast membrane chromatography coupled with liquid chromatography and time-of-flight mass spectrometry was developed to recognize and identify the specific active components from traditional Chinese medicines. Primary rat osteoblasts were used for the preparation of the stationary phase in the cell chromatography method. Retention components from the cell chromatography were collected and analyzed by liquid chromatography with time-of-flight mass spectrometry. This method was applied in screening active components from extracts of four traditional Chinese medicines. In total, 24 potentially active components with different structures were retained by osteoblast cell chromatography. There were five phenolic glucosides and one triterpenoid saponin from Curculigo orchioides Gaertn, two organic acids and ten flavonoids from Epimedium sagittatum Maxim, one phthalide compound and one organic acid from Angelica sinensis Diels, and two flavonoids and two saponins from Anemarrhena asphodeloides Bunge. Among those, four components (icariin, curculigoside, ferulaic acid, and timosaponin BII) were used for in vitro pharmacodynamics validation. They significantly increased the osteoblast proliferation, alkaline phosphatase activity, levels of bone gla protein and collagen type 1, and promoted mineralized nodule formation. The developed method was an effective screening method for finding active components from complex medicines that act on bone diseases.
['Wang|Nani|N|', 'Zhang|Qiaoyan|Q|', 'Xin|Hailiang|H|', 'Shou|Dan|D|http://orcid.org/0000-0003-3465-4072', 'Qin|Luping|L|']
D000818:Animals D002462:Cell Membrane D002853:Chromatography, Liquid D004365:Drugs, Chinese Herbal D013058:Mass Spectrometry D008516:Medicine, Chinese Traditional D010006:Osteoblasts D064209:Phytochemicals D051381:Rats
osteoblasts cell membrane chromatography traditional Chinese medicines drug screening osteogenesis