Vaginal prevalence of human papillomavirus infections in women with uterovaginal aplasia before and after laparoscopically assisted creation of a neovagina: a prospective epidemiological observational study.
OBJECTIVE
To study vaginal as opposed to cervical human papillomavirus (HPV) acquisition with regard to true prevalence, HPV types, and the role of co-factors in virgins and after their sexual debut.


DESIGN
Prospective epidemiological observational study.


SETTING
University hospital specialised in genital malformations.


POPULATION
Women diagnosed with Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS) and undergoing neovaginoplasty between November 2011 and July 2017.


METHODS
This is a prospective study including 186 women with MRKHS before and after sexual debut.


MAIN OUTCOME MEASURES
Conventional vaginal cytology and different HPV tests were performed at surgery and during routine gynaecological follow-up 1, 3, 6 and ≥ 11 months after surgery and risk factors were documented.


RESULTS
The mean age of all women at surgery was 20.1 years (SD 5.4), mean body mass index (BMI) was 22.1 kg/m2 (SD 4.6). In 83 vaginal samples from 41 different women at least one of the HPV tests was positive. Thirty-three different HPV types were detected. The prevalence of 41/186 = 22.0% as well as type distribution are comparable with those found in a young German female population. The overall rate of acquisition was clearly associated with sexual activity and smoking habits. Out of 367 Papanicolaou smears only six were abnormal with Pap IIID (MN II) and no obvious vaginal lesion was detected.


CONCLUSIONS
Vaginal HPV prevalence and HPV types in previously virgin women after creation of a neovagina are not different from the acquisition of cervical infections in the general population and is clearly associated with sexual activity and with smoking habits. However, abnormal Papanicolaou smears are rarely seen.


TWEETABLE ABSTRACT
Vaginal HPV prevalence after creation of a neovagina is similar to that on the cervix in the general population.
['Rall|K K|KK|', 'Schoenfisch|B|B|', 'Schoeller|D|D|', 'Stefanescu|D|D|', 'Koelle|A|A|', 'Henes|M|M|', 'Huebner|M|M|', 'Taran|F A|FA|', 'Seeger|H|H|', 'Iftner|A|A|', 'Iftner|T|T|', 'Brucker|S Y|SY|']
D058489:46, XX Disorders of Sex Development D000293:Adolescent D000328:Adult D000013:Congenital Abnormalities D005260:Female D006801:Humans D009095:Mullerian Ducts D065006:Papanicolaou Test D030361:Papillomavirus Infections D015995:Prevalence D019651:Reconstructive Surgical Procedures D012307:Risk Factors D012725:Sexual Behavior D012907:Smoking D014621:Vagina D055815:Young Adult
prevalence Human papillomavirus neovagina Mayer-Rokitansky-Küster-Hauser syndrome uterovaginal agenesis