Human herpesvirus 8 glycoprotein B binds the entry receptor DC-SIGN.
We have previously shown that human herpesvirus 8 (HHV-8) uses DC-SIGN as an entry receptor for dendritic cells, macrophages and B cells. The viral attachment protein for DC-SIGN is unknown. HHV-8 virions contain five conserved herpesvirus glycoproteins, a single unique glycoprotein, and two predicted glycoproteins. Previous studies have shown that DC-SIGN binds highly mannosylated glycoproteins. The HHV-8 glycoprotein B (gB) has been reported to be highly mannosylated, and therefore we hypothesized that gB will bind to DC-SIGN. In this report we confirm that gB has a high mannose carbohydrate structure and demonstrate for the first time that it binds DC-SIGN in a dose-dependent manner. We also identify key amino acids in the DC-SIGN carbohydrate recognition domain that are required for HHV-8 infection and compare these results with published binding regions for ICAM-2/3 and HIV-1 gp120. These results clarify some of the initial events in HHV-8 entry and can be used for the design of targeted preventive therapies.
['Hensler|Heather R|HR|', 'Tomaszewski|Monica J|MJ|', 'Rappocciolo|Giovanna|G|', 'Rinaldo|Charles R|CR|', 'Jenkins|Frank J|FJ|']
D015815:Cell Adhesion Molecules D006566:Herpesviridae Infections D019288:Herpesvirus 8, Human D006801:Humans D037181:Lectins, C-Type D011485:Protein Binding D017434:Protein Structure, Tertiary D011956:Receptors, Cell Surface D011991:Receptors, Virus D014759:Viral Envelope Proteins
Human herpesvirus 8 DC-SIGN