Elevated Serum MicroRNA Let-7c in Moyamoya Disease.
BACKGROUND
Few studies have examined the relationship between mircroRNAs and moyamoya disease (MMD). We performed a study of the significance of let-7c expression in the serum of MMD patients.


METHODS
The experimental group includes 49 MMD patients, and the control group consists of 30 normal people, 20 cerebral hemorrhage patients, 20 massive cerebral infarction patients, 20 nonmassive cerebral infarction patients, and 20 neurological autoimmune disease patients. Let-7 family levels were determined by polymerase chain reaction. A dual luciferase assay was used to test whether let-7c recognized the 3'UTR of RNF213.


RESULTS
The expression level of let-7c in MMD patients is higher than that observed in the control groups (P < .001). The luciferase assay results indicated that hsa-let-7c could diminish luciferase activity from a reporter vector containing the 3'-UTR of RNF213 (P < .05). The suppression of luciferase activity is not found in mutRNF213 (P > .05).


CONCLUSIONS
Increased expression of let-7c in MMD patients may contribute to MMD pathogenesis by targeting RNF213. Thus, let-7c may be a potential biomarker for the diagnosis of MMD.
['Zhao|Shaoyun|S|', 'Gong|Zhe|Z|', 'Zhang|Jing|J|', 'Xu|Xiaoge|X|', 'Liu|Peidong|P|', 'Guan|Wenjuan|W|', 'Jing|Lijun|L|', 'Peng|Tao|T|', 'Teng|Junfang|J|', 'Jia|Yanjie|Y|']
D000328:Adult D001327:Autoimmune Diseases D002543:Cerebral Hemorrhage D002544:Cerebral Infarction D005260:Female D057809:HEK293 Cells D006801:Humans D008297:Male D035683:MicroRNAs D008875:Middle Aged D009072:Moyamoya Disease D012333:RNA, Messenger D018709:Statistics, Nonparametric D014162:Transfection
let-7c Moyamoya disease RNF213 PCR