The roles of metastasis-related proteins in the development of giant cell tumor of bone, osteosarcoma and Ewing's sarcoma.
BACKGROUND
Giant cell tumor of bone (GC), osteosarcoma (OS) and Ewing's sarcoma (ES) are three different types of bone cancer with common and specific pathology features.


OBJECTIVE
The purpose of the study was to examine the relationship and differences of the three bone tumors using clinical samples.


METHODS
Through screening the profiles of clinical samples from GC, OS and ES patients using a humanoncology array, we found 26, 25 and 15 tumorigenesis factors significantly increased in GS, OS and ES tissues compared to normal individuals. eNOS, endostatin, HIF-1α, IL-6, CCL2/MCP-1, CCL8/MCP-2, CCL7/MCP-3, Tie and VEGF directly or indirectly involve in the metastasis Therefore, expression levels of the 6 factors were further determined by Western blot.


RESULTS
The results showed levels of MCP1, MCP2, MCP3 or IL-6 in the GS, OS and ES significantly increased, and the expression levels of angiogenesis and anti-angiogenesis factors containing eNOS, endostatin, HIF-1α, Tie or VEGF were enhanced.


CONCLUSIONS
Our results suggest that eNOS, endostatin, HIF-1α, IL-6, CCL2/MCP-1, CCL8/MCP-2, CCL7/MCP-3, Tie and VEGF may play important roles in tumorigenesis, reveal the expression differences of tumor-associated cytokines and angiogenesis related factors, and provide clinical evidence for studying the mechanisms on the metastasis in GC, OS and ES.
['Dou|Bo|B|', 'Chen|Tianrui|T|', 'Chu|Qiubo|Q|', 'Zhang|Guirong|G|', 'Meng|Zhaoli|Z|']
D015153:Blotting, Western D001859:Bone Neoplasms D018212:Giant Cell Tumor of Bone D006801:Humans D012516:Osteosarcoma D012512:Sarcoma, Ewing
Giant cell tumor of bone osteosarcoma metastasis Ewing’s sarcoma