An efficient single-cell based method for linking human T cell phenotype to T cell receptor sequence and specificity.
Single-cell antigen-receptor gene amplification and sequencing platforms have been used to characterize T cell receptor (TCR) repertoires but typically fail to generate paired full-length gene products for direct expression cloning and do not enable linking this data to cell phenotype information. To overcome these limitations, we established a high-throughput platform for the quantitative and qualitative analysis of human TCR repertoires that provides insights into the clonal and functional composition of human CD4+ and CD8+ αβ T cells at the molecular and cellular level. The strategy is a powerful tool to qualitatively assess differences between antigen receptors of phenotypically defined αβ T cell subsets, e.g. in immune responses to cancer, vaccination, or infection, and in autoimmune diseases.
['Wahl|Ilka|I|', 'Hoffmann|Sandro|S|', 'Hundsdorfer|Rebecca|R|', 'Puchan|Julia|J|', 'Hoffman|Stephen L|SL|', 'Kremsner|Peter G|PG|', 'Mordmüller|Benjamin|B|', 'Busse|Christian E|CE|', 'Wardemann|Hedda|H|']
phenotype T cell receptor expression cloning functional TCR analysis single cell