Platinum compounds for high-resolution in vivo cancer imaging.
Platinum(II) compounds, principally cisplatin and carboplatin, are commonly used front-line cancer therapeutics. Despite their widespread use and continued interest in the development of new derivatives, including nanoformulations with improved properties, it has been difficult to visualize platinum compounds in live subjects, in real time, and with subcellular resolution. Here, we present four novel cisplatin- and carboplatin-derived fluorescent imaging compounds for quantitative intravital cancer imaging. We conjugated 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-daiza-s-indacene (BODIPY) to Pt(II) complexes to generate derivatives with robust in vivo fluorescence and retained DNA-damaging and cytotoxic properties. We successfully applied these compounds to image pharmacokinetics and tumor uptake in a xenograft cancer mouse model. By using a genetic reporter of single-cell DNA damage for in vivo imaging, Pt drug accumulation and resultant DNA damage could be monitored in individual tumor cells, at subcellular resolution, and in real time in a live animal model of cancer. These derivatives represent promising imaging tools that will be useful in understanding further the distribution and interactions of platinum within tumors.
['Miller|Miles A|MA|', 'Askevold|Bjorn|B|', 'Yang|Katherine S|KS|', 'Kohler|Rainer H|RH|', 'Weissleder|Ralph|R|']
D000818:Animals D000970:Antineoplastic Agents D001896:Boron Compounds D045744:Cell Line, Tumor D056831:Coordination Complexes D004249:DNA Damage D005455:Fluorescent Antibody Technique D005456:Fluorescent Dyes D006801:Humans D051379:Mice D008819:Mice, Nude D009369:Neoplasms D010984:Platinum D011859:Radiography D014183:Transplantation, Heterologous
pharmacokinetics DNA damage fluorescent probes intravital imaging agents pharmacology