miR-342-5p inhibits osteosarcoma cell growth, migration, invasion, and sensitivity to Doxorubicin through targeting Wnt7b.
Osteosarcoma (OS) accounts for 9 percent of cancer-related deaths in young people. The PI3K/Akt signaling, a well-known carcinogenic signaling pathway in human cancer, cooperates with other signaling pathways such as Wnt signaling to promote cancer progression. Wnt7b, as a transforming member of the Wnt family, could activate mTORC1 through PI3K-AKT signaling and is upregulated in OS. In the present study, we found that miR-342-5p inhibits Wnt7b expression via direct binding to Wnt7b 3'-UTR. miR-342-5p overexpression remarkably suppressed the viability and invasion while enhanced the apoptosis of OS cells; meanwhile, Wnt7b, β-catenin, c-myc, and cyclin D1 proteins were reduced while E-cadherin protein showed to be increased. Consistent with its expression pattern, Wnt7b exerted oncogenic effects on OS cells. Wnt7b could significantly attenuate the impacts of miR-342-5p. In conclusion, we demonstrated a miR-342-5p/Wnt7b axis that regulates the capacity of OS cells to proliferate and to invade through Wnt/β-catenin signaling. The miR-342-5p/Wnt7b axis might be novel targets for OS targeted therapy, which needs further in vivo and clinical investigations.
['Liu|Qing|Q|', 'Wang|Zhenting|Z|', 'Zhou|Xiaohua|X|', 'Tang|Mingying|M|', 'Tan|Wei|W|', 'Sun|Tianshi|T|', 'Deng|Youwen|Y|']
D017209:Apoptosis D063646:Carcinogenesis D002453:Cell Cycle D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D004317:Doxorubicin D019008:Drug Resistance, Neoplasm D058750:Epithelial-Mesenchymal Transition D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008297:Male D035683:MicroRNAs D009361:Neoplasm Invasiveness D012516:Osteosarcoma D051153:Wnt Proteins D060449:Wnt Signaling Pathway D051176:beta Catenin
miR-342-5p Wnt7b Osteosarcoma (OS) Wnt/β-catenin signaling