Process Analysis of Anaerobic Fermentation Exposure to Metal Mixtures.
Anaerobic fermentation is a cost-effective biowaste disposal approach. During fermentation, microorganisms require a trace amount of metals for optimal growth and performance. This study investigated the effects of metal mixtures on biogas properties, process stability, substrate degradation, enzyme activity, and microbial communities during anaerobic fermentation. The addition of iron (Fe), nickel (Ni), and zinc (Zn) into a copper (Cu)-stressed fermentation system resulted in higher cumulative biogas yields, ammonia nitrogen (NH4+-N) concentrations and coenzyme F420 activities. Ni and Zn addition enhanced process stability and acetate utilization. The addition of these metals also improved and brought forward the peak daily biogas yields as well as increased CH4 content to 88.94 and 86.58%, respectively. Adding Zn into the Cu-stressed system improved the abundance of Defluviitoga, Fibrobacter and Methanothermobacter, the degradation of cellulose, and the transformation of CO2 to CH4. The bacterial and archaeal communities were responsible for the degradation of lignocelluloses and CH4 production during the fermentation process. This study supports the reutilization of heavy metal-contaminated biowaste and provides references for further research on heavy metals impacted anaerobic fermentation.
['Tian|Yonglan|Y|', 'Zhang|Huayong|H|0000-0001-8898-8567', 'Zheng|Lei|L|', 'Li|Shusen|S|', 'Hao|He|H|', 'Yin|Meixiao|M|', 'Cao|Yudong|Y|', 'Huang|Hai|H|']
D000693:Anaerobiosis D001673:Biodegradation, Environmental D056804:Biofuels D019149:Bioreactors D004785:Environmental Pollutants D005285:Fermentation D019216:Metals, Heavy D008697:Methane D064307:Microbiota D012037:Refuse Disposal
enzyme activity microbial communities heavy metals biogas production methanogens substrate biodegradation