In vitro and in vivo assessment of lactic acid-modified chitosan scaffolds for potential treatment of full-thickness burns.
Autologous skin transplantation is today's "gold standard" treatment for full-thickness burns. However, when > 30% of total body surface area is damaged, there is an important shortage of autologous donor sites for skin grafting; then, treatment alternatives become crucial. Such alternatives can be based on polymeric scaffolds capable of functioning as protective covers and cells/factors carriers. Chitosan (CTS) is a natural-derived polymer with relevant biological-related properties but poor mechanical performance. Improved mechanical properties can be achieved through lactic acid grafting (LA-g); nevertheless, LA-g affects the biological response towards the CTS-based materials. In this work, CTS-LA scaffolds with different LA-g percentages were synthesized and evaluated to determine appropriate LA-g degrees for full-thickness burns treatment. In vitro results indicated that the higher the LA-g percentage, the lower the capability of the scaffolds to sustain fibroblasts culture. Scaffolds with LA-g around 28% (CTS-LA28) sustained cell culture and allowed normal cell functionality. Further evaluation of CTS-LA28 as acellular and cellular grafts in a full-thickness burn mouse model showed that at 28 days post-burn, macroscopic characteristic of the reparation tissue were closer to healthy skin when cellular grafts were used for treatment; histological evaluation also showed that dermis cellularity and collagenous fibers structure were similar to those in healthy skin when cellular grafts were used for burns treatment. © 2017 Wiley Periodicals Inc. J Biomed Mater Res Part A: 105A: 2875-2891, 2017.
['Velasquillo|Cristina|C|', 'Silva-Bermudez|Phaedra|P|http://orcid.org/0000-0001-6830-3321', 'Vázquez|Nadia|N|', 'Martínez|Alan|A|', 'Espadín|Andres|A|', 'García-López|Julieta|J|', 'Medina-Vega|Antonio|A|', 'Lecona|Hugo|H|', 'Pichardo-Baena|Raúl|R|', 'Ibarra|Clemente|C|', 'Shirai|Keiko|K|']
D000818:Animals D001672:Biocompatible Materials D002056:Burns D002478:Cells, Cultured D048271:Chitosan D005347:Fibroblasts D006801:Humans D019344:Lactic Acid D008297:Male D051379:Mice D008819:Mice, Nude D012867:Skin D054457:Tissue Scaffolds D014945:Wound Healing
chitosan full-thickness burns lactic acid fibroblasts