Effect of high temperature on immune response of grass carp (Ctenopharyngodon idellus) by transcriptome analysis.
Exposure to high temperature is an inherent feature of grass carp culture in southern China and juvenile grass carps are predisposed to infectious disease in this condition. To understand how high temperature impacts the immune response to pathogens in grass carp, the transcriptomic profiles of the spleens from immune injected grass carp groups undergoing heat stress and normal temperature were investigated. An average of 72 million clean reads per library was obtained, and approximate 80% of these genes were successfully mapped to the reference genome. A total of 2287 up-regulated and 1068 down-regulated genes were identified. 10 immune-related categories involving 90 differently expressed genes were scrutinized. Expression patterns of 18 differentially expressed genes involving in immune response were validated by quantitative real-time RT-PCR. These results provide further significant insights into the influence mechanism of high temperature to immune response in grass carp.
['Yang|Ying|Y|', 'Yu|Hui|H|', 'Li|Hua|H|', 'Wang|Anli|A|', 'Yu|Hai-Yi|HY|']
D016980:Aeromonas hydrophila D000818:Animals D002347:Carps D018076:DNA, Complementary D005393:Fish Diseases D029941:Fish Proteins D020869:Gene Expression Profiling D005786:Gene Expression Regulation D016905:Gram-Negative Bacterial Infections D006358:Hot Temperature D007113:Immunity, Innate D011897:Random Allocation D060888:Real-Time Polymerase Chain Reaction D017423:Sequence Analysis, RNA
High temperature Immune response Transcriptomic Infectious disease Ctenopharygodon idella