PtpA, a secreted tyrosine phosphatase from Staphylococcus aureus, contributes to virulence and interacts with coronin-1A during infection.
Secretion of bacterial signaling proteins and adaptation to the host, especially during infection, are processes that are often linked in pathogenic bacteria. The human pathogen Staphylococcus aureus is equipped with a large arsenal of immune-modulating factors, allowing it to either subvert the host immune response or to create permissive niches for its survival. Recently, we showed that one of the low-molecular-weight protein tyrosine phosphatases produced by S. aureus, PtpA, is secreted during growth. Here, we report that deletion of ptpA in S. aureus affects intramacrophage survival and infectivity. We also observed that PtpA is secreted during macrophage infection. Immunoprecipitation assays identified several host proteins as putative intracellular binding partners for PtpA, including coronin-1A, a cytoskeleton-associated protein that is implicated in a variety of cellular processes. Of note, we demonstrated that coronin-1A is phosphorylated on tyrosine residues upon S. aureus infection and that its phosphorylation profile is linked to PtpA expression. Our results confirm that PtpA has a critical role during infection as a bacterial effector protein that counteracts host defenses.
['Gannoun-Zaki|Laila|L|', 'Pätzold|Linda|L|', 'Huc-Brandt|Sylvaine|S|', 'Baronian|Grégory|G|', 'Elhawy|Mohamed Ibrahem|MI|', 'Gaupp|Rosmarie|R|', 'Martin|Marianne|M|', 'Blanc-Potard|Anne-Béatrice|AB|', 'Letourneur|François|F|', 'Bischoff|Markus|M|', 'Molle|Virginie|V|']
D000818:Animals D001426:Bacterial Proteins D003001:Cloning, Molecular D004023:Dictyostelium D005260:Female D015870:Gene Expression D005786:Gene Expression Regulation D054884:Host-Pathogen Interactions D051379:Mice D008810:Mice, Inbred C57BL D008840:Microfilament Proteins D010766:Phosphorylation D011485:Protein Binding D017027:Protein Tyrosine Phosphatases D000067996:RAW 264.7 Cells D011994:Recombinant Proteins D015398:Signal Transduction D013203:Staphylococcal Infections D013211:Staphylococcus aureus D014443:Tyrosine D014774:Virulence
Staphylococcus aureus (S. aureus) bacterial pathogenesis bacterial protein phosphatase coronin host-pathogen interaction protein phosphorylation