DNA induced sequestration of a bioactive cationic fluorophore from the lipid environment: A spectroscopic investigation.
The effect of calf-thymus DNA (ctDNA) on the lipid bound probe, formed by the cationic phenazinium dye phenosafranin (PSF) and the anionic lipid dimyristoyl-L-α-phosphatidylglycerol (DMPG), has been unearthed exploiting various spectroscopic techniques. Steady state and time-resolved fluorometric studies and measurements of circular dichroism and DNA helix melting temperature reveal that in the presence of DNA the probe is dislodged from the lipid environment and gets intercalated within the DNA helix. The work qualitatively illustrates that the anionic lipid can be used as a potential nanocarrier for delivering the cationic drugs to the most relevant biomacromolecular target, DNA.
['Ghosh|Saptarshi|S|', 'Kundu|Pronab|P|', 'Chattopadhyay|Nitin|N|']
D000818:Animals D002412:Cations D002417:Cattle D002942:Circular Dichroism D004247:DNA D005456:Fluorescent Dyes D010619:Phenazines D010715:Phosphatidylglycerols D044366:Transition Temperature
Lipid ctDNA Circular dichroism Helix melting Fluorescence Phenosafanin