Mechanistic study for the simultaneous determination of metformin and teneligliptin in human plasma using hydrophilic interaction liquid chromatography-MS/MS.
AIM
A simple, selective and sensitive hydrophilic interaction liquid chromatography-MS/MS method is developed for the simultaneous determination of metformin (MET) and teneligliptin (TEN) in human plasma using deuterated internal standards. The mechanism of retention of analytes was studied by varying the proportion of organic diluent, buffer strength, pH of the mobile phase and temperature.


RESULTS
The results showed a mixed-mode mechanism comprising of hydrophilic (partition) and electrostatic interaction (ion exchange) for MET and essentially hydrophilic for TEN. The linear calibration curves were established in the concentration range of 1.0-1000 ng/ml for MET and 0.50-750 ng/ml for TEN.


CONCLUSION
The method was applied to determine plasma concentration of MET and TEN in healthy subjects.
['Shah|Priyanka A|PA|', 'Shrivastav|Pranav S|PS|', 'Vanol|Pravin G|PG|', 'Sanyal|Mallika|M|']
D002853:Chromatography, Liquid D054873:Dipeptidyl-Peptidase IV Inhibitors D006801:Humans D057927:Hydrophobic and Hydrophilic Interactions D007004:Hypoglycemic Agents D008687:Metformin D010949:Plasma D011720:Pyrazoles D053719:Tandem Mass Spectrometry D053778:Thiazolidines
metformin teneligliptin hydrophilic interaction electrostatic interaction diabetes enthalpy exothermic hydrophilic interaction liquid chromatography retention mechanism van't Hoff equation