Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?
This study aimed to evaluate the effect of different photobiomodulation (PBM) radiant exposures on the viability, proliferation, and gene expression of pulp fibroblasts from human primary teeth (HPF) involved in the pulp tissue repair. HPF were irradiated with Laser InGaAlP (Twin Flex Evolution, MMOptics®) at 660-nm wavelength (red); single time, continuous mode, 0.04-cm2 laser tip area, and 0.225-cm laser tip diameter, keeping the distance of 1 mm between the laser beam and the cell culture. The doses used were between 1.2 and 6.2 J/cm2 and were evaluated at the 6 h, 12 h, and 24 h after PBM. MTT and crystal violet assays evaluated the cell viability and proliferation. RT-PCR verified VEGF and FGF-2 mRNA expression. A blinded examiner analyzed the data through two-way ANOVA followed by Tukey test (p < 0.05). The groups with higher powers (10 mW, 15 mW, 20 mW, and 25 mW), shortest application periods (10 s), and radiant exposures between 2.5 and 6.2 J/cm2 exhibited statistically higher viability than that of the groups with small power (5 mW), longer application period (50 s), and radiant exposure of 6.2 J/cm2 (p < 0.05). VEGF and FGF-2 mRNA expression were observed at the three evaluated periods (6 h, 12 h, and 24 h) and the highest expression was in the shortest period (p < 0.05). All radiant exposures maintained HPF viable. The period of 6 h after irradiation showed statistically greater gene expression for both growth factors than other periods. VEGF mRNA had no differences among the dosimetries studied. The best radiant exposures for FGF-2 gene expression were 2.5 J/cm2 and 3.7 J/cm2.
['Bergamo|Mariel Tavares|MT|', 'Vitor|Luciana Lourenço Ribeiro|LLR|', 'Dionísio|Thiago José|TJ|', 'Marques|Nádia Carolina Teixeira|NCT|', 'Oliveira|Rodrigo Cardoso|RC|', 'Ambrosio|Eloá Cristina Passucci|ECP|', 'Sakai|Vivien Thiemy|VT|', 'Santos|Carlos Ferreira|CF|', 'Lourenço Neto|Natalino|N|', 'Machado|Maria Aparecida Andrade Moreira|MAAM|', 'Oliveira|Thais Marchini|TM|http://orcid.org/0000-0003-3460-3144']
D049109:Cell Proliferation D002470:Cell Survival D003782:Dental Pulp D006801:Humans D028022:Low-Level Light Therapy D014094:Tooth, Deciduous
Dental pulp Fibroblast Fibroblast growth factor-2 Low-level light therapy Vascular endothelial growth factor A