Human iPSC-derived blood-brain barrier microvessels: validation of barrier function and endothelial cell behavior.
Microvessels of the blood-brain barrier (BBB) regulate transport into the brain. The highly specialized brain microvascular endothelial cells, a major component of the BBB, express tight junctions and efflux transporters which regulate paracellular and transcellular permeability. However, most existing models of BBB microvessels fail to exhibit physiological barrier function. Here, using (iPSC)-derived human brain microvascular endothelial cells (dhBMECs) within templated type I collagen channels we mimic the cylindrical geometry, cell-extracellular matrix interactions, and shear flow typical of human brain post-capillary venules. We characterize the structure and barrier function in comparison to non-brain-specific microvessels, and show that dhBMEC microvessels recapitulate physiologically low solute permeability and quiescent endothelial cell behavior. Transcellular permeability is increased two-fold using a clinically relevant dose of a p-glycoprotein inhibitor tariquidar, while paracellular permeability is increased using a bolus dose of hyperosmolar agent mannitol. Lastly, we show that our human BBB microvessels are responsive to inflammatory cytokines via upregulation of surface adhesion molecules and increased leukocyte adhesion, but no changes in permeability. Human iPSC-derived blood-brain barrier microvessels support quantitative analysis of barrier function and endothelial cell dynamics in quiescence and in response to biologically- and clinically-relevant perturbations.
['Linville|Raleigh M|RM|', 'DeStefano|Jackson G|JG|', 'Sklar|Matt B|MB|', 'Xu|Zinnia|Z|', 'Farrell|Alanna M|AM|', 'Bogorad|Max I|MI|', 'Chu|Chengyan|C|', 'Walczak|Piotr|P|', 'Cheng|Linzhao|L|', 'Mahairaki|Vasiliki|V|', 'Whartenby|Katharine A|KA|', 'Calabresi|Peter A|PA|', 'Searson|Peter C|PC|']
D001812:Blood-Brain Barrier D002199:Capillary Permeability D002454:Cell Differentiation D002460:Cell Line D042783:Endothelial Cells D004867:Equipment Design D006801:Humans D057026:Induced Pluripotent Stem Cells D055806:Microvessels D023822:Tissue Engineering
Blood-brain barrier Microvessels Brain microvascular endothelial cells Permeability Induced pluripotent stem cells Tissue engineering