Saccharin fading is not required for the acquisition of alcohol self-administration, and can alter the dynamics of cue-alcohol memory reconsolidation.
RATIONALE
Animal models of alcohol-seeking are useful for understanding alcohol addiction and for treatment development, but throughput in these models is limited by the extensive pretraining required to overcome the aversive taste of ethanol. Work by Augier et al. (Psychopharmacology 231: 4561-4568, 2014) indicates that Wistar rats will self-administer alcohol without water deprivation, exposure to sweetened ethanol solutions or intermittent access to ethanol.


OBJECTIVES AND METHODS
We sought to replicate and extend the work of Augier et al. by comparing the acquisition of instrumental self-administration of ethanol in Lister-Hooded rats that had been previously saccharin faded (SF group) or not (NSF group). We also aimed to determine whether NMDA receptor antagonism with MK-801, given at memory reactivation, reduced subsequent ethanol-seeking behaviour in both groups of animals. Finally, we assessed the ethanol preference of SF and NSF rats using the two-bottle choice procedure.


RESULTS
Both SF and NSF groups acquired instrumental self-administration of ethanol, though SF rats consumed fewer of the earned reinforcers. MK-801, given at memory reactivation, had different effects on NSF and SF rats: impairing the capacity of an ethanol-paired conditioned stimulus (CS) to support reinstatement in NSF rats, and enhancing it in SF rats. Finally, neither SF nor NSF rats showed a preference for ethanol.


CONCLUSIONS
Our data support those of Augier et al. (Psychopharmacology 231: 4561-4568, 2014) that pretraining is unnecessary for rats to acquire instrumental self-administration of ethanol. Indeed, saccharin fading may produce a weaker memory that extinguishes more readily, thus accounting for the different effects of MK-801 on SF and NSF rats.
['Puaud|Mickaël|M|', 'Ossowska|Zofia|Z|', 'Barnard|Jordan|J|', 'Milton|Amy L|AL|']
D000428:Alcohol Drinking D000818:Animals D003216:Conditioning, Operant D003463:Cues D016291:Dizocilpine Maleate D000431:Ethanol D008297:Male D008568:Memory D051381:Rats D017208:Rats, Wistar D016194:Receptors, N-Methyl-D-Aspartate D012439:Saccharin D012646:Self Administration
Alcohol Memory reconsolidation Rat NMDA receptor antagonism Drug self-administration