New insights in the clinical and translational relevance of miR483-5p in adrenocortical cancer.
Adrenocortical cancer (ACC) is a rare aggressive malignancy. Recent ACC integrated genomics analysis contributed to redefine the risk groups on molecular basis, including tumor microRNAs (miRs), detectable also in the bloodstream. We developed a quantitative real-time (RT) assay for the measurement of miR483 and miR483-5p absolute levels in plasma samples. miR483/miR483-5p levels were evaluated in plasma samples of 27 patients with ACC before surgery and at follow-up. Statistically significant differences in miR483-5p and miR483 levels were found between stage 1/2 and stage 3/4 ACCs in pre-surgery and post-surgery samples. ROC curve analysis of miR483-5p levels gave a prediction of the clinical stage (accuracy 0.917±0.084), with the best cut-off value of 0.221 ng/ml, prognosticating overall and recurrence-free survival. In a multivariate Cox analysis (HR 16.2, 95%CI[1.39-188.6, P<0.026]), miR483-5p was the only variable that significantly predicted recurrence, but not overall survival. In addition, miR483 and miR483-5p levels correlated with the number of circulating tumor cells (CTCs) detected in the same blood samples, independently of the timing of sampling. In conclusion, we demonstrated that miR483-5p absolute plasma levels in ACC patients are powerful molecular markers that may help in the follow-up of patients after surgery and chemotherapy, and contribute to more accurately classify and predict tumor progression.
['Salvianti|Francesca|F|', 'Canu|Letizia|L|', 'Poli|Giada|G|', 'Armignacco|Roberta|R|', 'Scatena|Cristian|C|', 'Cantini|Giulia|G|', 'Di Franco|Alessandra|A|', 'Gelmini|Stefania|S|', 'Ercolino|Tonino|T|', 'Pazzagli|Mario|M|', 'Nesi|Gabriella|G|', 'Mannelli|Massimo|M|', 'Pinzani|Pamela|P|', 'Luconi|Michaela|M|']
adrenal carcinoma liquid biopsy miRNA quantitative real time RT-PCR rare tumor