Intestinal iontophoresis from mucoadhesive patches: a strategy for oral delivery.
Biologics have limited permeability across the intestine and are prone to degradation in the acidic-proteolytic milieu of the gastrointestinal tract, leading to poor oral bioavailability. Iontophoresis is a promising technology that can substantially improve transport of drugs across biological barriers and has been particularly explored for skin. In this study, we investigated whether iontophoresis across the intestine can be utilized to improve oral insulin transport. Application of electric current to intestinal cells resulted in opening of the tight junctions in vitro and a consequent about 3-fold improvement in paracellular transport of insulin. When evaluated in vivo using insulin-loaded mucoadhesive patches, iontophoresis produced profound hypoglycemia (63% blood glucose drop in 3 h) without damaging the intestinal tissue and the efficacy depended on insulin dose and current density. This study presents a proof of principle for intestinal iontophoresis as a novel method for oral protein delivery.
['Banerjee|Amrita|A|', 'Chen|Renwei|R|', 'Arafin|Shamsul|S|', 'Mitragotri|Samir|S|']
D000268:Adhesiveness D000284:Administration, Oral D000818:Animals D001692:Biological Transport D001786:Blood Glucose D018938:Caco-2 Cells D002626:Chemistry, Pharmaceutical D016503:Drug Delivery Systems D005456:Fluorescent Dyes D006801:Humans D007328:Insulin D007413:Intestinal Mucosa D007478:Iontophoresis D061848:Optical Imaging D010539:Permeability D051381:Rats D012867:Skin
Intestinal iontophoresis Insulin Oral drug delivery