Prodrug encapsulated albumin nanoparticles as an alternative approach to manifest anti-proliferative effects of suicide gene therapy.
Conventional anticancer agents are associated with limited therapeutic efficacy and substantial nonspecific cytotoxicity. Thus, there is an imminent need for an alternative approach that can specifically annihilate the cancer cells with minimal side effects. Among such alternative approaches, CD::UPRT (cytosine deaminase uracil phosphoribosyl transferase) suicide gene therapy has tremendous potential due to its high efficacy. Prodrug 5-Fluorocytosine (5-FC) used in combination with CD::UPRT suicide gene suffers from limited solubility which subsequently leads to decline in therapeutic efficacy. In order to overcome this, 5-FC encapsulated bovine serum albumin nanoparticles (BSA-5-FC NPs) were prepared in this work by desolvation method. Physico-chemical characterizations studies revealed amorphous nature of BSA-5-FC NPs with uniform spherical morphology. Apart from increase in solubility, encapsulated 5-FC followed slow and sustained release profile. Suicide gene expressing stable clone of L-132 cells were adapted for investigating therapeutic potential of BSA-5-FC NPs. These nanoparticles were readily taken up by the cells in a concentration dependent manner and subsequently manifested apoptosis, which was further confirmed by morphological examination and gene expression analysis. These findings clearly illustrate that CD::UPRT suicide gene therapy can be efficiently utilized in combination with this nanosystem for improved suicide gene therapy and tumor eradication.
['Tirkey|Bulbul|B|', 'Bhushan|Bharat|B|', 'Uday Kumar|S|S|', 'Gopinath|P|P|']
D000818:Animals D002417:Cattle D002460:Cell Line D049109:Cell Proliferation D002470:Cell Survival D065546:Drug Liberation D000067493:Dynamic Light Scattering D004705:Endocytosis D005434:Flow Cytometry D005437:Flucytosine D005786:Gene Expression Regulation D041722:Genes, Transgenic, Suicide D015316:Genetic Therapy D006801:Humans D053758:Nanoparticles D011355:Prodrugs D012710:Serum Albumin, Bovine D017550:Spectroscopy, Fourier Transform Infrared D013194:Staining and Labeling D055672:Static Electricity D013696:Temperature D014961:X-Ray Diffraction
Prodrug Albumin nanoparticles Suicide gene therapy 5-FC Apoptosis Cancer therapy