Optimum extraction of polysaccharides from Opuntia dillenii and evaluation of its antioxidant activities.
Response surface methodology (RSM) was applied to optimize the extraction of crude polysaccharides from Opuntia dillenii (Ker-Gaw) Haw. A three-level, four-variable Box-Behnken design was employed to obtain the best possible combination of extraction temperature (80-90 °C), extraction time (50-70 min), number of extraction cycle (1-3 times), and ratio of water to raw material (8:1-12:1, v/w) for maximum yield of crude polysaccharide. Besides, the antioxidant capacity of crude polysaccharide was evaluated by DPPH assay. The results showed that optimized extraction conditions were extraction temperature 85 °C, extraction time 63.7 min, extraction 2 times and ratio of water to raw material 11.14:1. Under these conditions, the experimental yield was 27.36 ± 0.21%, which is well in close agreement with the value (27.44%) predicted by RSM model. Pharmacological test showed that O. dillenii crude polysaccharides had a good antioxidant activity.
['Yang|Qin|Q|', 'Chen|Huaguo|H|', 'Zhou|Xin|X|', 'Zhang|Junzeng|J|']
D000704:Analysis of Variance D000975:Antioxidants D029747:Opuntia D011134:Polysaccharides D015203:Reproducibility of Results D012997:Solvents D013696:Temperature D013997:Time Factors D014867:Water
Extraction Polysaccharides Opuntia dillenii Antioxidant activity Response surface methodology