Incubation at 32.5°C and above causes malformations in the zebrafish embryo.
Zebrafish embryos are increasingly used for developmental toxicity screening of candidate drugs and are occasionally co-incubated with a metabolic activation system at 32°C for 1, 2 or 4h, depending on their developmental stage. As this temperature is higher than the optimal temperature for zebrafish embryonic development (26-28.5°C), we investigated whether continuous incubation of zebrafish embryos from 2.5 until 96h post fertilization (hpf) at high temperatures (30.5-36.5°C) causes malformations. At 32.5°C tail malformations were observed as early as 24hpf, and these became even more prominent at 34.5 and 36.5°C. Cardiovascular and head malformations, edema and blood accumulations throughout the body were present at 36.5°C. Finally, temperatures higher than 28.5°C accelerated embryonic development except for 36.5°C, at which a lower hatching rate and hatching enzyme activity were observed. In conclusion, incubation of zebrafish embryos at 32.5°C and above from 2.5 until 96hpf causes malformations as early as 24hpf.
['Pype|Casper|C|', 'Verbueken|Evy|E|', 'Saad|Moayad A|MA|', 'Casteleyn|Christophe R|CR|', 'Van Ginneken|Chris J|CJ|', 'Knapen|Dries|D|', 'Van Cruchten|Steven J|SJ|']
D000818:Animals D001681:Biological Assay D056668:Cathepsin L D004625:Embryo, Nonmammalian D013696:Temperature D013997:Time Factors D018675:Toxicity Tests D015027:Zebrafish D029961:Zebrafish Proteins
Malformation Zebrafish Embryo Temperature Development Hatching enzyme Morphology