Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated Isothermal Amplification on a Lab-On-A-Disc Platform.
Lab-on-a-disc (LOD) has emerged as a promising candidate for a point-of-care testing (POCT) device because it can effectively integrate complex fluid manipulation steps using multiple layers of polymeric substrates. However, it is still highly challenging to design and fabricate temperature measurement and heating system in non-contact with the surface of LOD, which is a prerequisite to successful realization of DNA amplification especially with a rotatable disc. This study presents a Lab-on-a-disc (LOD)-based automatic loop-mediated isothermal amplification (LAMP) system, where a thermochromic coating (<~420 µm) was used to distantly measure the chamber's temperature and a micro graphite film was integrated into the chamber to remotely absorb laser beam with super high efficiency. We used a deep learning network to more consistently analyze the product of LAMP than we could with the naked eye. Consequently, both temperature heating and measurement were carried out without a physical contact with the surface of LOD. The experimental results show that the proposed approach, which no previous work has attempted, was highly effective in realizing LAMP in LOD.
['Lim|Da Ye Seul|DYS|', 'Seo|Moo-Jung|MJ|', 'Yoo|Jae Chern|JC|']
D001831:Body Temperature D003124:Colorimetry D006801:Humans D056656:Lab-On-A-Chip Devices D016571:Neural Networks, Computer D000067716:Point-of-Care Testing D013696:Temperature
convolutional neural network loop-mediated isothermal amplification lab-on-a-disc point-of-care testing hydroxy naphthol blue