miR-140-5p suppresses BMP2-mediated osteogenesis in undifferentiated human mesenchymal stem cells.
Human mesenchymal stem cells (hMSCs) have self-renewal and differentiation capabilities but the regulatory mechanisms of MSC fate determination remain poorly understood. Here, we aimed to identify microRNAs enriched in hMSCs that modulate differentiation commitments. Microarray analysis revealed that miR-140-5p is commonly enriched in undifferentiated hMSCs from various tissue sources. Moreover, bioinformatic analysis and luciferase reporter assay validated that miR-140-5p directly represses bone morphogenic protein 2 (BMP2). Furthermore, blocking miR-140-5p in hMSCs increased the expression of BMP signaling components and critical regulators of osteogenic differentiation. We propose that miR-140-5p functionally inhibits osteogenic lineage commitment in undifferentiated hMSCs.
['Hwang|Supyong|S|', 'Park|Seul-Ki|SK|', 'Lee|Ha Yeon|HY|', 'Kim|Seong Who|SW|', 'Lee|Jung Shin|JS|', 'Choi|Eun Kyung|EK|', 'You|Dalsan|D|', 'Kim|Choung-Soo|CS|', 'Suh|Nayoung|N|']
D055396:Bone Morphogenetic Protein 2 D002454:Cell Differentiation D019070:Cell Lineage D005786:Gene Expression Regulation D006801:Humans D059630:Mesenchymal Stem Cells D035683:MicroRNAs D010012:Osteogenesis D015398:Signal Transduction
miR-140-5p Human mesenchymal stem cells Differentiation Bone morphogenic protein 2 Osteogenic lineage commitment