Nitric Oxide-Enhanced Molecular Imaging of Atheroma using Vascular Cellular Adhesion Molecule 1-Targeted Echogenic Immunoliposomes.
The aim of this study was to determine whether pre-treatment with nitric oxide-loaded echogenic liposomes (NO-ELIP) plus ultrasound can improve highlighting by molecularly targeted (anti-vascular cell adhesion molecule 1 [VCAM-1]) ELIP of atheroma components. Atherosclerotic animals were treated with anti-VCAM-1-ELIP or immunoglobulin (IgG)-ELIP. Each group was selected at random to receive pre-treatment with standard ELIP plus ultrasound, NO-ELIP without ultrasound and NO-ELIP plus ultrasound. Intravascular ultrasound highlighting data for the same arterial segments were collected before and after treatment. Pre-treatment with NO-ELIP plus ultrasound resulted in a significant increase in acoustic enhancement by anti-VCAM-1-ELIP (21.3 ± 1.5% for gray-scale value, 53.9 ± 3.1% for radiofrequency data; p < 0.001 vs. IgG-ELIP, p < 0.05 vs. pre-treatment with standard ELIP plus ultrasound or NO-ELIP without ultrasound). NO-ELIP plus ultrasound can improve highlighting of atheroma by anti-VCAM-1 ELIP. This NO pre-treatment strategy may be useful in optimizing contrast agent delivery to the vascular wall for both diagnostic and therapeutic applications.
['Kim|Hyunggun|H|', 'Kee|Patrick H|PH|', 'Rim|Yonghoon|Y|', 'Moody|Melanie R|MR|', 'Klegerman|Melvin E|ME|', 'Vela|Deborah|D|', 'Huang|Shao-Ling|SL|', 'McPherson|David D|DD|', 'Laing|Susan T|ST|']
D000818:Animals D004195:Disease Models, Animal D008081:Liposomes D057054:Molecular Imaging D009569:Nitric Oxide D058226:Plaque, Atherosclerotic D013552:Swine D013556:Swine, Miniature D014463:Ultrasonography D019010:Vascular Cell Adhesion Molecule-1
Molecular imaging Atheroma Ultrasound Contrast agent Contrast echocardiography Early atherosclerosis Nitric oxide Vascular cell adhesion molecule