Lysobacter silvestris sp. nov., isolated from alpine forest soil, and reclassification of Luteimonas tolerans as Lysobacter tolerans comb. nov.
A Gram-stain-negative, rod-shaped, motile, catalase-positive and cytochrome c oxidase-positive bacterial strain, designated AM20-91T, was isolated from alpine forest soil. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain AM20-91T was related to the genus Lysobacter and had highest 16S rRNA gene sequence similarities to the type strains of Lysobacter novalis THG-PC7T (97.8 %), Luteimonas tolerans UM1T (97.7 %) and Lysobacter ximonensis XM415T (97.0 %). The strain contained ubiquinone 8 as the predominant respiratory quinone; its polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminophospholipids. The major cellular fatty acids (>10 %) were iso-C15 : 0, iso-C11 : 0 3-OH and iso-C11 : 0. The DNA G+C content was 63.35 % (draft genome sequence). The combined results of phylogenetic, phenotypic, DNA-DNA relatedness and chemotaxonomic analyses demonstrated that strain AM20-91T represents a novel species of the genus Lysobacter, for which the name Lysobacter silvestris sp. nov. is proposed. The type strain is AM20-91T (=DSM 104734T=LMG 30011). In this study, it is also proposed that Luteimonas tolerans be reclassified as member of the genus Lysobacter.
['Margesin|Rosa|R|', 'Zhang|De-Chao|DC|', 'Albuquerque|Luciana|L|', 'Froufe|Hugo J C|HJC|', 'Egas|Conceição|C|', 'da Costa|Milton S|MS|']
D015373:Bacterial Typing Techniques D001482:Base Composition D004269:DNA, Bacterial D005227:Fatty Acids D065928:Forests D007558:Italy D053528:Lysobacter D009693:Nucleic Acid Hybridization D010743:Phospholipids D010802:Phylogeny D012336:RNA, Ribosomal, 16S D017422:Sequence Analysis, DNA D012988:Soil Microbiology D014451:Ubiquinone
Lysobacter alpine forest Luteimonas