Does the Apparent Diffusion Coefficient Value Predict Permanent Cerebral Ischemia/Reperfusion Injury in Rats?
RATIONALE AND OBJECTIVES
Variation in tissue damage after cerebral ischemia/reperfusion (I/R) can cause uncertainty in stroke-related studies, which can be reduced if the damage can be predicted early after ischemia by measuring the apparent diffusion coefficient (ADC). We investigated whether ADC measurement in the acute phase can predict permanent cerebral I/R injury.


MATERIALS AND METHODS
The middle cerebral artery occlusion model was established using the intraluminal suture method to induce 60 minutes of ischemia followed by reperfusion in rats. T2-weighted images and diffusion-weighted images were obtained at 30 minutes and 24 hours after ischemia. Neuronal cell survival was assessed by neuronal nuclei (NeuN) immunofluorescence staining. The correlation between relative ADC (rADC) values at 30 minutes and I/R injury at 24 hours after ischemia was analyzed. Magnetic resonance imaging results were confirmed by histologic analysis.


RESULTS
The correlation between rADC values at 30 minutes and 24 hours was strong in the ischemic core and peri-infarct region but moderate in the anterior choroidal and hypothalamic region. Histologic analysis revealed that the correlation between rADC values at 30 minutes and the number of NeuN-positive cells at 24 hours was strong in the ischemic core and peri-infarct region but moderate in the anterior choroidal and hypothalamic region. Furthermore, there was a strong positive correlation between the sum of rADC values of three regions at 30 minutes and the infarct volume at 24 hours.


CONCLUSION
ADC measurement in the acute phase can predict permanent cerebral I/R injury and provide important information for the evaluation of ischemic stroke.
['Kwon|Jae-Im|JI|', 'Woo|Chul-Woong|CW|', 'Kim|Kyung-Won|KW|', 'Choi|Yoonseok|Y|', 'Kim|Sang-Tae|ST|', 'Kim|Yun Jae|YJ|', 'Kang|Jeeheon|J|', 'Lee|Do-Wan|DW|', 'Tak|Eunyoung|E|', 'Kim|Jeong-Kon|JK|', 'Jung|Seung-Chae|SC|', 'Kim|Tae-Hyung|TH|', 'Woo|Dong-Cheol|DC|']
D000818:Animals D002545:Brain Ischemia D038524:Diffusion Magnetic Resonance Imaging D004195:Disease Models, Animal D008297:Male D011237:Predictive Value of Tests D051381:Rats D017207:Rats, Sprague-Dawley D015427:Reperfusion Injury
Apparent diffusion coefficient Prediction Permanent cerebral ischemia/reperfusion injury