Prolidase-dependent mechanism of (Z)-8,9-epoxyheptadeca-1,11,14-triene-induced inhibition of collagen biosynthesis in cultured human skin fibroblasts.
The effects of polyolefinic compound from roots of Cirsium palustre, (Z)-8,9-epoxyheptadeca-1,11,14-triene (EHT) on collagen biosynthesis, prolidase activity, expression of insulin-like growth factor receptor (IGF-IR), β1 integrin, MAP kinases (pERK1/2), the transcription factors such as nuclear factor kappa B (NF-κB) and hypoxia-inducible factor-1α (HIF-1α) were evaluated in human dermal fibroblasts treated with micromolar concentrations (40-200 μM) for 24 h. It was found that EHT-dependent inhibition of collagen biosynthesis was accompanied by parallel inhibition in prolidase activity. Since IGF-I is the most potent regulator of both processes and prolidase is regulated by β1 integrin signalling, the effect of EHT on IGF-IR and β1 integrin receptor expressions were evaluated. Exposure of the cells to EHT contributed to distinct increase in IGF-IR and slight increase in β1 integrin receptor expressions. It was accompanied by decrease in expression of pERK1/2, HIF-1α and NF-κB. EHT-dependent inhibition of collagen biosynthesis results from inhibition of prolidase activity, the enzyme involved in collagen biosynthesis.
['Szoka|Lukasz|L|', 'Karna|Ewa|E|', 'Nazaruk|Jolanta|J|', 'Palka|Jerzy A|JA|']
D000475:Alkenes D002478:Cells, Cultured D031180:Cirsium D003094:Collagen D004150:Dipeptidases D004852:Epoxy Compounds D048049:Extracellular Signal-Regulated MAP Kinases D005347:Fibroblasts D006801:Humans D051795:Hypoxia-Inducible Factor 1, alpha Subunit D007334:Insulin-Like Growth Factor I D019012:Integrin beta1 D015394:Molecular Structure D016328:NF-kappa B D009822:Oils, Volatile D010938:Plant Oils D018517:Plant Roots D011090:Polyenes D017451:Receptors, Somatomedin
collagen (Z)-8,9-epoxyheptadeca-1,11,14-triene prolidase insulin-like growth factor receptor MAP kinases nuclear factor kappa B hypoxia-inducible factor-1α