Synergistic effects of adipose-derived mesenchymal stem cells and selenium nanoparticles on streptozotocin-induced memory impairment in the rat.
AIMS
Memory impairment is determined to be the most well-known symptom of Alzheimer's disease (AD). Although cell therapy seems is an efficient therapeutic strategy to attenuate the AD-related memory impairment, transplanted cells have a short lifespan and do not survive long term in the recipient animals. Herein, we investigated whether the combination therapy of Selenium nanoparticles (SeNPs) and stem cells attenuates the neurotoxicity in an AD animal model.


MATERIAL AND METHODS
The adipose-derived mesenchymal stem cells (AMSCs) were transplanted in the AD model. In addition to cell injections, the animals also received oral administration of SeNPs (0.4 mg/kg) for one month. Recognition memory, cell survival, and BDNF concentration were assessed using the novel object recognition task, immunofluorescence, and ELISA methods.


KEY FINDINGS
Our results showed that the combined therapy was more effective in increasing the discrimination index than the administering SeNPs or AMSCs alone. Moreover, SeNPs and stem cells together had the greatest effects in reducing the deposition of Aβ and increasing the concentration of BDNF. Ultimately, the survival and proliferation of transplanted cells were more in the group that received stem cells besides SeNPs.


SIGNIFICANCE
Taken together, it seems that the transplantation of MSCs combined with SeNPs could achieve better results in the neuroprotection in the AD model than a conventional treatment of SeNPs or stem cells alone.
['Gholamigeravand|Bahareh|B|', 'Shahidi|Siamak|S|', 'Afshar|Simin|S|', 'Gholipour|Parsa|P|', 'Samzadeh-Kermani|Alireza|A|', 'Amiri|Kimia|K|', 'Majidi|Mahsa|M|', 'Abbasalipourkabir|Roghayeh|R|', 'Arabestani|Mohammad Reza|MR|', 'Soleimani Asl|Sara|S|']
D000544:Alzheimer Disease D000818:Animals D004195:Disease Models, Animal D008297:Male D008569:Memory Disorders D045164:Mesenchymal Stem Cell Transplantation D059630:Mesenchymal Stem Cells D053768:Metal Nanoparticles D000066829:Neuroprotection D051381:Rats D017208:Rats, Wistar D012643:Selenium D013234:Stem Cells D013311:Streptozocin
Memory impairment BDNF Deposition of Aβ AMSCs, SeNPs