CRISPR/Cas-mediated Fubp1 silencing disrupts circadian oscillation of Per1 protein via downregulating Syncrip expression.
Most living organisms have physiological and behavioral circadian rhythms controlled by molecular clocks. In mammals, several core clock genes show self-perpetuating oscillation profiles of their messenger RNAs (mRNAs) and proteins through an auto-regulatory transcription-translation feedback loop (TTFL). As a critical component in the molecular clock system, Period 1 (Per1) contributes to the maintenance of circadian rhythm duration predominantly in peripheral clocks. Alterations in Per1 expression and oscillating patterns lead to the development of cancers as well as circadian rhythm abnormalities. In this study, we demonstrate that the phasic profile of Per1 protein was clearly disrupted in CRISPR/Cas-mediated Fubp1-deficient cells. Although Fubp1 does not show rhythmic expression, Fubp1 upregulates the mRNA and protein level of Syncrip, the main post-transcriptional regulator of Per1 protein oscillation. In addition to the diverse physiological functions of Fubp1, including cell-cycle regulation and cellular metabolic control, our results suggest new roles for Fubp1 in the molecular clock system.
['Kim|Tae-Jun|TJ|', 'Sung|Jae Hun|JH|', 'Shin|Jae-Cheon|JC|', 'Kim|Do-Yeon|DY|http://orcid.org/0000-0003-2200-7396']
D000818:Animals D064113:CRISPR-Cas Systems D002940:Circadian Rhythm D004268:DNA-Binding Proteins D015536:Down-Regulation D005786:Gene Expression Regulation D020868:Gene Silencing D034441:Heterogeneous-Nuclear Ribonucleoproteins D051379:Mice D041681:NIH 3T3 Cells D056950:Period Circadian Proteins D012333:RNA, Messenger D016601:RNA-Binding Proteins
Fubp1 oscillation Per1 Syncrip circadian rhythm CRISPR/Cas