Oxidative Epigallocatechin Gallate Coating on Polymeric Substrates for Bone Tissue Regeneration.
Plant derived flavonoids have not been well explored in tissue engineering applications due to difficulties in efficient formulations with biomaterials for controlled presentation. Here, the authors report that surface coating of epigallocatechin gallate (EGCG) on polymeric substrates including poly (L-lactic acid) (PLLA) nanofibers can be performed via oxidative polymerization of EGCG in the presence of cations, enabling regulation of biological functions of multiple cell types implicated in bone regeneration. EGCG coating on the PLLA nanofiber promotes osteogenic differentiation of adipose-derived stem cells (ADSCs) and is potent to suppress adipogenesis of ADSCs while significantly reduces osteoclastic maturation of murine macrophages. Moreover, EGCG coating serves as a protective layer for ADSCs against oxidative stress caused by hydrogen peroxide. Finally, the in vivo implantation of EGCG-coated nanofibers into a mouse calvarial defect model significantly promotes the bone regeneration (61.52 ± 28.10%) as compared to defect (17.48 ± 11.07%). Collectively, the results suggest that EGCG coating is a simple bioinspired surface modification of polymeric biomaterials and importantly can thus serve as a promising interface for tuning activities of multiple cell types associated with bone fracture healing.
['Madhurakkat Perikamana|Sajeesh Kumar|SK|', 'Lee|Sang Min|SM|', 'Lee|Jinkyu|J|', 'Ahmad|Taufiq|T|', 'Lee|Min Suk|MS|', 'Yang|Hee Seok|HS|', 'Shin|Heungsoo|H|0000-0002-9036-155X']
D000273:Adipose Tissue D000818:Animals D001861:Bone Regeneration D002392:Catechin D002454:Cell Differentiation D020099:Coated Materials, Biocompatible D051379:Mice D008813:Mice, Inbred ICR D057139:Nanofibers D010010:Osteoclasts D011091:Polyesters D000067996:RAW 264.7 Cells D012886:Skull D013234:Stem Cells
surface modification bone tissue engineering multifunctionality plant polyphenols