Evaluation of phytochemical analysis and antimicrobial activities Allium essential oil against the growth of some microbial pathogens.
In this experimental study, the Allium essential oil (AHEO) was extracted through the hydrodistillation method. AHEO components were identified through gas chromatography/mass spectrometry (GC/MS), and its antioxidant properties and total phenolic content were examined through the methods of 2,2-diphenyl-1-picrylhydrazyl (DPPH), ß-carotene/linoleic acid inhibition and Folin-Ciocalteu, respectively. The antimicrobial effect of AHEO was evaluated on Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Streptococcus pyogenes, and Candida albicans through the methods of well diffusion, disk diffusion, minimum inhibitory concentration, and minimum bactericidal/fungicidal concentration. Phytochemical constituents (alkaloids, tannins, saponins, flavone and glycosides) were evaluated. 5-chloroorcylaldehyde with a percentage of 45.6% was the major compound of AHEO. Increasing concentration of AHEO had a significant effect (p ≤ 0.05) on inhibition zone diameter. The MICs of the AHEO varied from 0.25 mg/ml to 2 mg/ml. The MBCs/MFCs of the AHEO varied from 0.25 mg/ml to 4 mg/ml. The results of phytochemical screening of AHEO showed the existence of alkaloids, tannins, saponins, flavone and glycosides. There was also little difference between disk diffusion and well diffusion methods, and the data was well distributed throughout the X and Y components.
['Alizadeh Behbahani|Behrooz|B|', 'Imani Fooladi|Abbas Ali|AA|']
D000490:Allium D000890:Anti-Infective Agents D000975:Antioxidants D001409:Bacillus cereus D001412:Bacillus subtilis D002176:Candida albicans D052978:Disk Diffusion Antimicrobial Tests D004926:Escherichia coli D009822:Oils, Volatile D064209:Phytochemicals D010936:Plant Extracts D011550:Pseudomonas aeruginosa D013211:Staphylococcus aureus D013297:Streptococcus pyogenes
Phytochemical analysis Essential oil Microbial pathogens Antimicrobial effect