The AP2-type transcription factors DORNRÖSCHEN and DORNRÖSCHEN-LIKE promote G1/S transition.
The paralogous genes DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-type transcription factors that are expressed and act cell-autonomously in the central stem-cell zone or lateral organ founder cells (LOFCs) in the peripheral zone of the Arabidopsis shoot meristem (SAM), but their molecular contribution is unknown. Here, we show using the Arabidopsis thaliana MERISTEM LAYER 1 promoter that DRN and DRNL share a common function in cell cycle progression and potentially provide local competence for G1-S transitions in the SAM. Analysis of double transgenic DRN::erGFP and DRNL::erCERULEAN promoter fusion lines suggests that the trajectory of this cellular competence starts with DRN activity in the central stem-cell zone and extends locally via DRNL activity into groups of founder cells at the IM or FM periphery. Our data support the scenario that after gene duplication, DRN and DRNL acquired different transcription domains within the shoot meristem, but retained protein function that affects cell cycle progression, either centrally in stem cells or peripherally in primordial founder cells, a finding that is of general relevance for meristem function.
['Seeliger|Ingo|I|', 'Frerichs|Anneke|A|', 'Glowa|Dorothea|D|', 'Velo|Laura|L|', 'Comelli|Petra|P|', 'Chandler|John W|JW|', 'Werr|Wolfgang|W|http://orcid.org/0000-0002-6627-1685']
D017360:Arabidopsis D029681:Arabidopsis Proteins D049109:Cell Proliferation D059585:G1 Phase Cell Cycle Checkpoints D055786:Gene Knockout Techniques D018398:Homeodomain Proteins D018519:Meristem D018520:Plant Shoots D011401:Promoter Regions, Genetic D014157:Transcription Factors
Lateral organ founder cell Arabidopsis Cell cycle Shoot apical meristem