Pathological effects of processed bovine pericardial scaffolds--a comparative in vivo evaluation.
The objective of the present study was to assess the biocompatibility and regenerative potential of decellularized bovine pericardial scaffold in comparison with glutaraldehyde-treated and fresh bovine pericardial implants using short-term intramuscular implantation testing in a rat model. The inflammatory and immune responses were assessed using histopathological examination, special stains for connective tissue, histomorphometric evaluation, and immunohistochemistry. The decellularized pericardium showed an active tissue remodeling response with complete cellular invasion, minimum connective tissue encapsulation, extensive fibrovascular tissue formation, and collagen deposition. On the contrary, the glutaraldehyde-treated pericardial implants showed incomplete degradation and cellular invasion, while the fresh pericardial implants elicited a severe foreign body reaction. The results of immunohistochemical staining revealed a minimum T helper (CD4+) lymphocyte response in decellularized pericardial implants compared with its glutaraldehyde-treated and fresh counterparts. The decellularized bovine pericardium was better accepted as a prosthetic scaffold, which permitted maximum collagen deposition and active tissue remodeling by invading host cells and showed good tissue integration in vivo compared with glutaraldehyde-treated and fresh/untreated pericardium.
['Thampi|Parvathy|P|', 'Nair|Divakaran|D|', 'R|Lalithakunjamma|L|', 'N|Vijayan|V|', 'Venugopal|Syam|S|', 'Ramachandra|Umashankar|U|']
D000818:Animals D001672:Biocompatible Materials D015496:CD4-Positive T-Lymphocytes D018414:CD8-Positive T-Lymphocytes D002417:Cattle D003094:Collagen D005355:Fibrosis D005404:Fixatives D005549:Foreign-Body Reaction D005976:Glutaral D007150:Immunohistochemistry D008297:Male D009336:Necrosis D009389:Neovascularization, Pathologic D010496:Pericardium D051381:Rats D017208:Rats, Wistar D017568:Rectus Abdominis D023822:Tissue Engineering D016707:Tissue Fixation D054457:Tissue Scaffolds D014183:Transplantation, Heterologous
Biocompatibility Scaffold Decellularized bovine pericardium