Profiling of circulating microRNAs in patients with Barrett's esophagus and esophageal adenocarcinoma.
BACKGROUND
Circulating microRNAs (miRNAs) have been suggested as novel markers for various diseases. The goal of this pilot study was to identify circulating miRNAs differentially expressed comparing Barrett's esophagus (BE), esophageal adenocarcinoma (EAC), and controls.


METHODS
MicroRNA expression profiling was performed by qPCR array using plasma from six controls and eight BE and eight EAC patients. Validation was performed by analyzing the expression of six selected miRNAs, by qRT-PCR in 115 plasma samples of controls, BE, and EAC patients. Diagnostic accuracy was evaluated by area under the curve (AUC) analysis.


RESULTS
We identified three miRNAs that were elevated in EAC and four miRNAs that were elevated in BE. Further validation showed that miRNA-382-5p was significantly increased and miRNA-133a-3p significantly decreased in EAC. miRNA-194-5p and miRNA-451a were significantly increased and miRNA-136-5p significantly decreased in BE versus controls. A combination of three or more miRNAs was found to have a good diagnostic performance in discriminating BE from controls (AUC: 0.832), EAC from controls (AUC: 0.846), and BE from EAC (AUC: 0.797).


CONCLUSION
Our data suggest that circulating miRNAs are differentially expressed in BE and EAC. The miRNAs identified may be used for future non-invasive screening of BE and EAC.
['Bus|Pauline|P|', 'Kestens|Christine|C|', 'Ten Kate|Fiebo Jan Willem|FJ|', 'Peters|Wilbert|W|', 'Drenth|Joost Paulus Hubertus|JP|', 'Roodhart|Jeanine Merel Leonoor|JM|', 'Siersema|Peter Derk|PD|', 'van Baal|Jantine Wilhelmina Paula Maria|JW|']
D000230:Adenocarcinoma D000328:Adult D000368:Aged D001471:Barrett Esophagus D016022:Case-Control Studies D004938:Esophageal Neoplasms D005260:Female D006801:Humans D008297:Male D035683:MicroRNAs D008875:Middle Aged D010865:Pilot Projects D011237:Predictive Value of Tests D012372:ROC Curve
MicroRNA Esophageal adenocarcinoma Plasma Barrett’s esophagus Circulating biomarker