TEMPOL increases NAD(+) and improves redox imbalance in obese mice.
Continuous energy conversion is controlled by reduction-oxidation (redox) processes. NAD(+) and NADH represent an important redox couple in energy metabolism. 4-Hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL) is a redox-cycling nitroxide that promotes the scavenging of several reactive oxygen species (ROS) and is reduced to hydroxylamine by NADH. TEMPOL is also involved in NAD(+) production in the ascorbic acid-glutathione redox cycle. We utilized the chemical properties of TEMPOL to investigate the effects of antioxidants and NAD(+)/NADH modulators on the metabolic imbalance in obese mice. Increases in the NAD(+)/NADH ratio by TEMPOL ameliorated the metabolic imbalance when combined with a dietary intervention, changing from a high-fat diet to a normal diet. Plasma levels of the superoxide marker dihydroethidium were higher in mice receiving the dietary intervention compared with a control diet, but were normalized with TEMPOL consumption. These findings provide novel insights into redox regulation in obesity.
['Yamato|Mayumi|M|', 'Kawano|Kimika|K|', 'Yamanaka|Yuki|Y|', 'Saiga|Misako|M|', 'Yamada|Ken-Ichi|K|']
D000818:Animals D000975:Antioxidants D001205:Ascorbic Acid D003497:Cyclic N-Oxides D059305:Diet, High-Fat D004578:Electron Spin Resonance Spectroscopy D004734:Energy Metabolism D004996:Ethidium D005978:Glutathione D006801:Humans D051379:Mice D008820:Mice, Obese D009243:NAD D009765:Obesity D010084:Oxidation-Reduction D018384:Oxidative Stress D017382:Reactive Oxygen Species D013113:Spin Labels
NAD(+) Obesity Nitroxide Oxidative stress Redox status