Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae).
PREMISE OF THE STUDY
We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador.


METHODS AND RESULTS
Using next-generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii.


CONCLUSIONS
We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species.
['Morillo|Eduardo|E|', 'Buitron|Johanna|J|', 'Limongi|Ricardo|R|', 'Vignes|Helene|H|', 'Argout|Xavier|X|']
microsatellites Handroanthus billbergii Bignoniaceae next-generation sequencing (NGS)