New Delhi metallo-β-lactamase-producing Enterobacteriaceae, United States.
We characterized 9 New Delhi metallo-β-lactamase-producing Enterobacteriaceae (5 Klebsiella pneumoniae, 2 Escherichia coli, 1 Enterobacter cloacae, 1 Salmonella enterica serovar Senftenberg) isolates identified in the United States and cultured from 8 patients in 5 states during April 2009-March 2011. Isolates were resistant to β-lactams, fluoroquinolones, and aminoglycosides, demonstrated MICs ≤1 µg/mL of colistin and polymyxin, and yielded positive metallo-β-lactamase screening results. Eight isolates had blaNDM-1, and 1 isolate had a novel allele (blaNDM-6). All 8 patients had recently been in India or Pakistan, where 6 received inpatient health care. Plasmids carrying blaNDM frequently carried AmpC or extended spectrum β-lactamase genes. Two K. pneumoniae isolates and a K. pneumoniae isolate from Sweden shared incompatibility group A/C plasmids with indistinguishable restriction patterns and a common blaNDM fragment; all 3 were multilocus sequence type 14. Restriction profiles of the remaining New Delhi metallo-β-lactamase plasmids, including 2 from the same patient, were diverse.
['Rasheed|J Kamile|JK|', 'Kitchel|Brandon|B|', 'Zhu|Wenming|W|', 'Anderson|Karen F|KF|', 'Clark|Nancye C|NC|', 'Ferraro|Mary Jane|MJ|', 'Savard|Patrice|P|', 'Humphries|Romney M|RM|', 'Kallen|Alexander J|AJ|', 'Limbago|Brandi M|BM|']
D000900:Anti-Bacterial Agents D004755:Enterobacteriaceae D004756:Enterobacteriaceae Infections D006801:Humans D008826:Microbial Sensitivity Tests D058885:Multilocus Sequence Typing D010802:Phylogeny D010957:Plasmids D017422:Sequence Analysis, DNA D014481:United States D018440:beta-Lactam Resistance D001618:beta-Lactamases
Enterobacteriaceae United States New Delhi metallo-β-lactamase NDM antimicrobial resistance bacteria carbapenem resistance carbapenemase