Characterization of mouse spinal cord vascular network by means of synchrotron radiation X-ray phase contrast tomography.
High resolution Synchrotron-based X-ray Phase Contrast Tomography (XPCT) allows the simultaneous detection of three dimensional neuronal and vascular networks without using contrast agents or invasive casting preparation. We show and discuss the different features observed in reconstructed XPCT volumes of the ex vivo mouse spinal cord in the lumbo-sacral region, including motor neurons and blood vessels. We report the application of an intensity-based segmentation method to detect and quantitatively characterize the modification in the vascular networks in terms of reduction in experimental visibility. In particular, we apply our approach to the case of the experimental autoimmune encephalomyelitis (EAE), i.e. human multiple sclerosis animal model.
['Massimi|Lorenzo|L|', 'Fratini|Michela|M|', 'Bukreeva|Inna|I|', 'Brun|Francesco|F|', 'Mittone|Alberto|A|', 'Campi|Gaetano|G|', 'Spanò|Raffaele|R|', 'Mastrogiacomo|Milena|M|', 'de Rosbo|Nicole Kerlero|NK|', 'Bravin|Alberto|A|', 'Uccelli|Antonio|A|', 'Cedola|Alessia|A|']
D000818:Animals D001808:Blood Vessels D004681:Encephalomyelitis, Autoimmune, Experimental D005260:Female D021621:Imaging, Three-Dimensional D051379:Mice D013116:Spinal Cord D017356:Synchrotrons D014057:Tomography, X-Ray Computed
Spinal cord vascular network X-ray phase contrast tomography Vascular segmentation