An automated imaging system for radiation biodosimetry.
We describe here an automated imaging system developed at the Center for High Throughput Minimally Invasive Radiation Biodosimetry. The imaging system is built around a fast, sensitive sCMOS camera and rapid switchable LED light source. It features complete automation of all the steps of the imaging process and contains built-in feedback loops to ensure proper operation. The imaging system is intended as a back end to the RABiT-a robotic platform for radiation biodosimetry. It is intended to automate image acquisition and analysis for four biodosimetry assays for which we have developed automated protocols: The Cytokinesis Blocked Micronucleus assay, the γ-H2AX assay, the Dicentric assay (using PNA or FISH probes) and the RABiT-BAND assay.
['Garty|Guy|G|', 'Bigelow|Alan W|AW|', 'Repin|Mikhail|M|', 'Turner|Helen C|HC|', 'Bian|Dakai|D|', 'Balajee|Adayabalam S|AS|', 'Lyulko|Oleksandra V|OV|', 'Taveras|Maria|M|', 'Yao|Y Lawrence|YL|', 'Brenner|David J|DJ|']
D001331:Automation D048749:Cytokinesis D006657:Histones D006801:Humans D007091:Image Processing, Computer-Assisted D015162:Micronucleus Tests D048631:Micronucleus, Germline D011874:Radiometry
sCMOS γ-H2AX dicentrics fluorescence microscopy mBAND micronuclei