γ-Glutamyl cyclotransferase contributes to endometrial carcinoma malignant progression and upregulation of PD-L1 expression during activation of epithelial-mesenchymal transition.
Recent increases in the incidence of endometrial carcinoma represent a significant risk to women's health. We found that γ-glutamyl cyclotransferase (GGCT) was significantly up-regulated in endometrial carcinoma tissues and cells, which suggested that it may be a potential target for treatment of endometrial carcinoma. Furthermore, the impact of GGCT on proliferation, migration, and invasion of endometrial carcinoma has been demonstrated in vitro and in vivo using GGCT silencing and overexpression techniques. In addition, the epithelial-mesenchymal transition (EMT) was significantly inhibited in response to GGCT knockdown, which indicated that GGCT may contribute endometrial carcinoma malignancy during activation of the EMT. We also found that GGCT regulated PD-L1 expression during EMT activation. Furthermore, co-culture of endometrial carcinoma cells with CD8+ T lymphocytes showed that downregulation of PD-L1 expression following GGCT knockdown contributed to the killing activity of CD8+ T lymphocytes on endometrial carcinoma cells. In conclusion, our study showed that GGCT contributed to malignant progression and upregulation of PD-L1 expression of endometrial carcinoma, and may be a potential target for treatment of endometrial carcinoma.
['Xu|Shengjie|S|', 'Yang|Ye|Y|', 'Wang|Xiaoyun|X|', 'Liu|Xiangnan|X|', 'Jin|Chengjuan|C|', 'Ma|Mingjun|M|', 'Wu|Sufang|S|', 'Li|Yanli|Y|']
D000818:Animals D060890:B7-H1 Antigen D018414:CD8-Positive T-Lymphocytes D045744:Cell Line, Tumor D049109:Cell Proliferation D018920:Coculture Techniques D066264:Datasets as Topic D018450:Disease Progression D016889:Endometrial Neoplasms D004717:Endometrium D058750:Epithelial-Mesenchymal Transition D025461:Feedback, Physiological D005260:Female D015972:Gene Expression Regulation, Neoplastic D055785:Gene Knockdown Techniques D057809:HEK293 Cells D064368:Healthy Volunteers D006801:Humans D051379:Mice D009361:Neoplasm Invasiveness D061251:Primary Cell Culture D034741:RNA, Small Interfering D015854:Up-Regulation D023041:Xenograft Model Antitumor Assays D005722:gamma-Glutamylcyclotransferase
γ-Glutamyl cyclotransferase Endometrial carcinoma Epithelial-mesenchymal transition PD-1/PD-L1 pathway