Fabrication and characterization of Mg-doped chitosan-gelatin nanocompound coatings for titanium surface functionalization.
Titanium and its alloys have been widely used in clinic and achieved great success. Due to the bio-inertness of titanium surface, challenges still exit in some compromised conditions. The present study aimed to functionalize titanium surface with magnesium (Mg)-doped chitosan/gelatin (CS/G) nanocompound coatings via electrophoretic deposition (EPD). CS/G coatings loaded with different amount of magnesium were successfully prepared on titanium substrate via EPD. Physicochemical characterization of the coatings confirmed that magnesium ions were loaded into the coatings in a dose-dependent manner. XRD results demonstrated that co-deposition of magnesium influenced the crystallinity of the coatings, and a new crystalline substance presented, namely hydrated basic magnesium carbonate. Mechanical tests showed improved tensile and shear bond strength of the magnesium-doped coatings, while the excessively high magnesium concentration could eventually decrease the bonding strength. Sustained release of magnesium ion was detected by ICP-OES within 28 days. TEM images also displayed that nanoparticles could be released from the coatings. In vitro cellular response assays demonstrated that the Mg-doped nanocompound coatings could enhance the proliferation and osteogenic differentiation of MC3T3-E1 cells compared to CS/G coatings. Therefore, it could be concluded that Mg-doped CS/G nanocompound coatings were successfully fabricated on titanium substrates via EPD. It would be a promising candidate to functionalize titanium surface with such organic-inorganic nanocompound coatings.
['Cai|Xinjie|X|', 'Cai|Jing|J|', 'Ma|Kena|K|', 'Huang|Pin|P|', 'Gong|Lingling|L|', 'Huang|Dan|D|', 'Jiang|Tao|T|', 'Wang|Yining|Y|']
D016475:3T3 Cells D000818:Animals D002118:Calcium D002454:Cell Differentiation D048271:Chitosan D020099:Coated Materials, Biocompatible D005780:Gelatin D008274:Magnesium D051379:Mice D008855:Microscopy, Electron, Scanning D053758:Nanoparticles D010006:Osteoblasts D010012:Osteogenesis D019736:Prostheses and Implants D014025:Titanium
coating functionalization Magnesium chitosan gelatin electrophoretic deposition