Lipid bilayer coatings for rapid enzyme-linked immunosorbent assay.
The enzyme-linked immunosorbent assay (ELISA) is a widely used method for protein detection and relies on the specific capture of target proteins while minimizing the nonspecific binding of other interfering proteins and biomolecules. To prevent nonspecific binding events, blocking agents such as bovine serum albumin (BSA) protein, mixtures of proteins in media such as milk or serum, and/or surfactants are typically added to ELISA plates after probe attachment and before analyte capture. Herein, we developed a streamlined ELISA strategy in which readily prepared lipid nanoparticles are utilized as the blocking agent and are added together with the probe molecule to the ELISA plate, resulting in fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. These measurement capabilities were established for coronavirus disease-2019 (COVID-19) antibody detection in saline and human serum conditions and are broadly applicable for developing rapid ELISA diagnostics.
['Yoon|Bo Kyeong|BK|', 'Sut|Tun Naw|TN|', 'Yoo|Ki Yeol|KY|', 'Lee|Seung Hwa|SH|', 'Hwang|Youngkyu|Y|', 'Jackman|Joshua A|JA|', 'Cho|Nam-Joon|NJ|']
Enzyme-linked immunosorbent assay ELISA Blocking Lipid nanoparticle Diagnostic