Class I and II histone deacetylase expression in human chronic periodontitis gingival tissue.
BACKGROUND AND OBJECTIVE
Histone deacetylase inhibitors (HDACi) are being considered to treat chronic inflammatory diseases at low doses. Currently HDACi that are more specific are being developed to target particular HDACs; therefore, this study aimed to determine levels and distribution of class I and II HDAC in human gingival samples obtained from patients with chronic periodontitis.


MATERIAL AND METHODS
Gingival biopsies were obtained from patients with and without (mild inflammation, no bone loss) periodontitis. Total RNA was isolated for real-time quantitative polymerase chain reaction to determine expression of HDACs 1-10. Immunohistochemistry was used to determine protein distribution of HDACs 1, 5, 8 and 9. Factor VIII, CD3 and tartrate resistant acid phosphatase (TRAP) were detected in serial sections to identify blood vessels, lymphocytes, pre-osteoclasts and osteoclasts cells respectively. Tumour necrosis factor α (TNF-α) expression was also assessed.


RESULTS
mRNA for HDAC 1, 5, 8 and 9 were significantly upregulated in chronic periodontitis gingival tissues compared to non-periodontitis samples (p < 0.05). Significantly higher HDAC 1 protein expression was observed in chronic periodontitis samples (p < 0.05), and was associated with CD3, TRAP and TNF-α-positive cells. HDAC 1, 5, 8 and 9 were expressed strongly by the factor VIII-positive microvasculature in the chronic periodontitis gingival tissues.


CONCLUSIONS
HDAC 1, 5, 8 and 9 expression was higher in gingival tissues from patients with chronic periodontitis compared to non-periodontitis samples. Results suggest that these HDACs could therefore be targeted with specific acting HDACi.
['Cantley|M D|MD|', 'Dharmapatni|A A S S K|AA|', 'Algate|K|K|', 'Crotti|T N|TN|', 'Bartold|P M|PM|', 'Haynes|D R|DR|']
D055113:Chronic Periodontitis D005881:Gingiva D006655:Histone Deacetylases D006801:Humans D010010:Osteoclasts D014409:Tumor Necrosis Factor-alpha
histone deacetylases periodontitis inflammation immunohistochemistry