Iridoids and sfingolipids from Hedyotis diffusa.
Seven new compounds were isolated from the aerial part of Hedyotis diffusa, including three iridoid glycosides, hedyoiridoidside A - C (1-3), two cerebrosides, hedyocerenoside F (4) and G (5), and two new ceramides, hedyoceramide A (6) and B (7). And six known iridoid glycosides (8-13) were also obtained. Their structures were established by their physico-chemical constants and spectroscopic analysis. The cytotoxicity of all compounds against tumor cell lines of human cervical cancer HeLa, human leukemia HL-60, human lung cancer A459, human hepatoma HepG2, human gastric gland carcinoma BCG-823, human nasopharyngeal cancer CNE-2, human colon cancer HCT15, and human prostate cancer PC-3 were also evaluated in vitro. As a result, new compound 1 exhibited evident cytotoxicity to all tumor cell lines, and the IC50 values are from 9.5μM to 28.2μM, while new compound 2 exhibited evident cytotoxicity to Hela, HL-60, A459, HepG2, BGC-823, CNE-2, and HCT15, and the IC50 values are from 15.8μM to 26.2μM. Known compound 11 also exhibited evident cytotoxicity to HL-60, A459, HepG2, BGC-823, CNE-2, and HCT15, and the IC50 values are from 16.5μM to 40.4μM. New compounds 4-7 and known compounds 12 and 13 showed moderate cytotoxicity to some tumor cell lines.
['Wang|Changfu|C|', 'Xin|Ping|P|', 'Wang|Youzhi|Y|', 'Zhou|Xuegang|X|', 'Wei|Donghua|D|', 'Deng|Chengjie|C|', 'Sun|Shiqin|S|']
D000972:Antineoplastic Agents, Phytogenic D045744:Cell Line, Tumor D032064:Hedyotis D006801:Humans D057889:Iridoid Glycosides D015394:Molecular Structure D035261:Plant Components, Aerial D013107:Sphingolipids
Hedyotis diffusa Iridoid glycosides Cerebrosides Ceramides Cytotoxicity Asperuloside (PubChem CID: 84298) Asperulosidic acid (PubChem CID: 44423083) Geniposidic acid (PubChem CID: 443354) Scandoside (PubChem CID: 21602023)