Development of MoSe₂ Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia. coli Shiga Toxin DNA.
The present study was aimed to develop "fluorine doped" tin oxide glass electrode with a MoSe2 nano-urchin based electrochemical biosensor for detection of Escherichia. coli Shiga toxin DNA. The study comprises two conductive electrodes, and the working electrodes were drop deposited using MoSe2 nano-urchin, and DNA sequences specific to Shiga toxin Escherichia. coli. Morphological characterizations were performed using Fourier transforms infrared spectrophotometer; X-ray diffraction technique and scanning electron microscopy. All measurements were done using methylene blue as an electrochemical indicator. The proposed electrochemical geno-sensor showed good linear detection range of 1 fM⁻100 μM with a low detection limit of 1 fM where the current response increased linearly with Escherichia. coli Shiga toxin dsDNA concentration with R2 = 0.99. Additionally, the real sample was spiked with the dsDNA that shows insignificant interference. The results revealed that the developed sensing platform significantly improved the sensitivity and can provide a promising platform for effective detection of biomolecules using minute samples due to its stability and sensitivity.
['Narang|Jagriti|J|', 'Mishra|Annu|A|', 'Pilloton|Roberto|R|', 'Vv|Alekhya|A|', 'Wadhwa|Shikha|S|', 'Pundir|Chandra Shekhar|CS|', 'Khanuja|Manika|M|']
D015374:Biosensing Techniques D004269:DNA, Bacterial D055664:Electrochemical Techniques D004566:Electrodes D004926:Escherichia coli D008751:Methylene Blue D008982:Molybdenum D049329:Nanostructures D018036:Selenium Compounds D022621:Shiga Toxin D017550:Spectroscopy, Fourier Transform Infrared D017971:Tin Compounds
geno-sensor fluorine doped tin oxide (FTO) methylene blue (MB) molybdenum diselenide nano-urchin