ZIF-8@GMP-Tb nanocomplex for ratiometric fluorescent detection of alkaline phosphatase activity.
Luminescent metal-organic frameworks (LMOFs) and their functional materials with unique characteristics can provide the basis for the construction of new analytical techniques, which can meet the continuous demand for various fields. In this work, guanosine monophosphate (GMP), terbium ion (Tb3+) and zeolitic imidazolate framework-8 (ZIF-8) are self-assembled to form a ZIF-8@GMP-Tb nanocomplex, which can be utilized as a ratiometric fluorescent probe to monitor alkaline phosphatase (ALP) activity. Specifically, with adding ALP, the fluorescence intensity at 547 nm (one of the characteristic emission peaks of Tb3+) obviously decreased. Meanwhile, the conjugated structure of GMP increased the fluorescence of ZIF-8 (located at 330 nm). The possible mechanism was proposed through the characterization of the materials. Based on the variation of the emission peaks at 330 and 547 nm, the ratiometric fluorescent sensor of ALP has a linear range of 0.25-20 U/L. Moreover, applying this sensing system to the detection of ALP in the human serum sample and ALP inhibitor investigation possesses satisfactory results. This work provides a new perspective for the utilization of ZIF-8 and lanthanide ions in manufacturing simple and sensitive sensors.
['Ling|Yu|Y|', 'He|Lin Zhao|LZ|', 'Wan|Chu Chu|CC|', 'Han|Lei|L|', 'Wang|Xiao Hu|XH|', 'Xu|Zi Yi|ZY|', 'Li|Xiao Lin|XL|', 'Li|Nian Bing|NB|', 'Luo|Hong Qun|HQ|']
D000469:Alkaline Phosphatase D005456:Fluorescent Dyes D006157:Guanosine Monophosphate D006801:Humans D013725:Terbium D017641:Zeolites
Alkaline phosphatase GMP ZIF-8 Ratiometric fluorescent sensor Tb ion