Adenovirus-mediated downregulation of the ubiquitin ligase RNF8 sensitizes bladder cancer to radiotherapy.
The ubiquitin ligase RNF8 promotes the DNA damage response (DDR). We observed that the expression of RNF8 was increased in bladder cancer cells and that this change in RNF8 expression could be reversed by adenovirus-mediated shRNA treatment. Moreover, we found that RNF8 knockdown sensitized bladder cancer cells to radiotherapy, as demonstrated by reduced cell survival. Additionally, the absence of RNF8 induced a high rate of apoptosis and impaired double-strand break repair signaling after radiotherapy. Furthermore, experiments on nude mice showed that combining shRNF8 treatment with radiotherapy suppressed implanted bladder tumor growth and enhanced apoptotic cell death in vivo. Altogether, our results indicated that RNF8 might be a novel target for bladder cancer treatment.
['Zhao|Mei-Jun|MJ|', 'Song|Yan-Feng|YF|', 'Niu|Hai-Tao|HT|', 'Tian|Ying-Xia|YX|', 'Yang|Xu-Guang|XG|', 'Xie|Kun|K|', 'Jing|Yu-Hong|YH|', 'Wang|De-Gui|DG|']
D000256:Adenoviridae D000818:Animals D015153:Blotting, Western D003114:Colony-Forming Units Assay D004249:DNA Damage D004260:DNA Repair D004268:DNA-Binding Proteins D005455:Fluorescent Antibody Technique D005720:Gamma Rays D006801:Humans D007124:Immunoenzyme Techniques D008297:Male D051379:Mice D008807:Mice, Inbred BALB C D008819:Mice, Nude D034741:RNA, Small Interfering D014407:Tumor Cells, Cultured D044767:Ubiquitin-Protein Ligases D001749:Urinary Bladder Neoplasms D023041:Xenograft Model Antitumor Assays
RNF8 bladder cancer radiotherapy adenovirus gene therapy