Diagnostic utility of BNP, corin and furin as biomarkers for cardiovascular complications in type 2 diabetes mellitus patients.
CONTEXT
The number of patients with type 2 diabetes mellitus (T2DM) is progressively increasing, and diabetic cardiovascular complications have become a public health problem. Brain or B-type natriuretic peptide (BNP) is a cardiac hormone synthesized as a pre-pro-peptide. pro-BNP is produced by cleaving the signal peptide then two proprotein convertases, corin and furin cleave pro-BNP to form a biologically active hormone. Two corin single nucleotide polymorphisms (SNPs) have been reported to alter corin protein conformation and impair its biological activity.


OBJECTIVE
We aimed to investigate the potential role of corin and furin in comparison to BNP as biomarkers for predicting cardiovascular complications in T2DM patients. The association of corin gene SNPs with corin levels was also examined.


METHODS
Seventy-five subjects were recruited in this study, including 25 T2DM patients with complications, 25 T2DM patients without complications as well as 25 healthy subjects. Plasma BNP, corin and furin levels were measured using enzyme-linked immunosorbent assays. Two corin SNPs were genotyped using allele specific oligonucleotide-polymerase chain reaction.


RESULTS
Both furin and BNP were found to be more sensitive than corin (80% versus 56%, p = 0.008), whereas furin showed higher specificity when compared to BNP (96% versus 84%, p = 0.041) and corin (96% versus 64%, p < 0.0001) in predicting cardiovascular complications in T2DM patients. Corin SNPs are not associated with corin levels, neither in the entire study cohort nor in the subgroup of T2DM patients with cardiovascular complications (p > 0.05).


CONCLUSIONS
Furin may be useful, either alone or in combination with other biomarkers, for cardiovascular risk stratification assessment in T2DM patients.
['Fathy|Shadia A|SA|', 'Abdel Hamid|Fatma F|FF|', 'Zabut|Baker M|BM|', 'Jamee|Amal F|AF|', 'Ali|Mohamed A M|MA|', 'Abu Mustafa|Ayman M|AM|']
D000368:Aged D015415:Biomarkers D002318:Cardiovascular Diseases D016022:Case-Control Studies D003924:Diabetes Mellitus, Type 2 D005260:Female D045683:Furin D005787:Gene Frequency D005838:Genotype D006801:Humans D008297:Male D008875:Middle Aged D020097:Natriuretic Peptide, Brain D020641:Polymorphism, Single Nucleotide D011379:Prognosis D012372:ROC Curve D012697:Serine Endopeptidases
BNP corin furin cardiovascular complications type 2 diabetes mellitus