Efficacy of autologous serum tears for treatment of neuropathic corneal pain.
OBJECTIVE
Corneal nerve damage may result in neuropathic corneal pain (NCP). Autologous serum tears (AST) have been shown to results in nerve regeneration and may help alleviate corneal pain. This study aimed to evaluate the efficacy of AST in the treatment of NCP.


METHODS
This was a retrospective case-control study. Sixteen patients suffering from severe NCP and no current ocular surface disease were compared to 12 controls. In vivo confocal microscopy (IVCM) (HRT3/RCM; Heidelberg Engineering GmbH, Germany) of the central corneas was performed bilaterally. Change in pain severity (scale of 0-10), corneal nerve density, tortuosity, reflectivity and presence of beading and micro-neuromas before and after treatment were recorded.


RESULTS
All patients had severe pain, with a mean of 9.1 ± 0.2 (range 8-10). Subbasal nerves were significantly decreased before treatment as compared to controls, including total nerve length (10,935.5 ± 1264.3 vs. 24,714.4 ± 1056.2 μm/mm2; p < 0.0001) and total number of nerves (10.5 ± 1.4 vs. 28.6 ± 2.0; p < 0.0001), respectively. Morphologically, significantly increased reflectivity (2.9 ± 0.2 vs. 1.2 ± 0.1; p = 0.00008) and tortuosity (2.4 ± 0.2 vs. 1.7 ± 0.1; p = 0.001), both graded on a scale of 0-4, were noted. After a mean of 3.8 ± 0.5 months (range 1-8 months) of AST treatment, pain severity decreased to 3.1 ± 0.3 (range 0-4), (p < 0.0001). Further, IVCM demonstrated a significant improvement (p < 0.005) in total nerve length (17,351.3 ± 1395.6  μm/mm2) and number (15.1 ± 1.6), as well as significant decrease in reflectivity (2.4 ± 0.2; p = 0.001) and tortuosity (2.2 ± 0.2; p = 0.001).


CONCLUSION
IVCM demonstrates underlying alterations of the subbasal corneal nerve plexus in patients suffering from debilitating NCP. AST-induced nerve regeneration is seen following treatment with AST, which correlates with improvement in patient symptoms of NCP.
['Aggarwal|Shruti|S|', 'Colon|Clara|C|', 'Kheirkhah|Ahmad|A|', 'Hamrah|Pedram|P|']
D000328:Adult D000368:Aged D016022:Case-Control Studies D003315:Cornea D058447:Eye Pain D005260:Female D006801:Humans D008297:Male D018613:Microscopy, Confocal D008875:Middle Aged D009412:Nerve Fibers D009437:Neuralgia D009882:Ophthalmic Nerve D009883:Ophthalmic Solutions D059408:Pain Management D010147:Pain Measurement D010949:Plasma D012189:Retrospective Studies D013666:Tears
Autologous serum tears In vivo confocal microscopy Corneal allodynia Corneal nerve regeneration Corneal neuropathic pain