PERK-KIPK-KCBP signalling negatively regulates root growth in Arabidopsis thaliana.
The Arabidopsis proline-rich, extensin-like receptor-like kinases (PERKs) are a small group of receptor-like kinases that are thought to act as sensors at the cell wall through their predicted proline-rich extracellular domains. In this study, we focused on the characterization of a subclade of three Arabidopsis predicted PERK genes, PERK8, -9, and -10, for which no functions were known. Yeast two-hybrid interaction studies were conducted with the PERK8,- 9, and -10 cytosolic kinase domains, and two members of the Arabidopsis AGC VIII kinase family were identified as interacting proteins: AGC1-9 and the closely related kinesin-like calmodulin-binding protein (KCBP)-interacting protein kinase (KIPK). As KIPK has been identified previously as an interactor of KCBP, these interactions were also examined further and confirmed in this study. Finally, T-DNA mutants for each gene were screened for altered phenotypes under different conditions, and from these screens, a role for the PERK, KIPK, and KCBP genes in negatively regulating root growth was uncovered.
['Humphrey|Tania V|TV|', 'Haasen|Katrina E|KE|', 'Aldea-Brydges|May Grace|MG|', 'Sun|He|H|', 'Zayed|Yara|Y|', 'Indriolo|Emily|E|', 'Goring|Daphne R|DR|']
D017360:Arabidopsis D029681:Arabidopsis Proteins D018506:Gene Expression Regulation, Plant D018517:Plant Roots
signalling root growth kinesin-like calmodulin-binding protein KCBP-interacting protein kinase Proline-rich extensin-like receptor-like kinase sucrose.