Autograft microskin combined with adipose-derived stem cell enhances wound healing in a full-thickness skin defect mouse model.
OBJECTIVE
Autograft microskin transplantation has been widely used as a skin graft therapy in full-thickness skin defect. However, skin grafting failure can lead to a pathological delay wound healing due to a poor vascularization bed. Considering the active role of adipose-derived stem cell (ADSC) in promoting angiogenesis, we intend to investigate the efficacy of autograft microskin combined with ADSC transplantation for facilitating wound healing in a full-thickness skin defect mouse model.


MATERIAL AND METHODS
An in vivo full-thickness skin defect mouse model was used to evaluate the contribution of transplantation microskin and ADSC in wound healing. The angiogenesis was detected by immunohistochemistry staining. In vitro paracrine signaling pathway was evaluated by protein array and Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway, and protein-protein interaction network analysis.


RESULTS
Co-transplantation of microskin and ADSC potentiated the wound healing with better epithelization, smaller scar thickness, and higher angiogenesis (CD31) in the subcutaneous layer. We found both EGF and VEGF cytokines were secreted by microskin in vitro. Additionally, secretome proteomic analysis in a co-culture system of microskin and ADSC revealed that ADSC could secrete a wide range of important molecules to form a reacting network with microskin, including VEGF, IL-6, EGF, uPAR, MCP-3, G-CSF, and Tie-2, which most likely supported the angiogenesis effect as observed.


CONCLUSION
Overall, we concluded that the use of ADSC partially modulates microskin function and enhances wound healing by promoting angiogenesis in a full-thickness skin defect mouse model.
['Luo|Yuansen|Y|', 'Yi|Xiaoyou|X|', 'Liang|Tangzhao|T|', 'Jiang|Shihai|S|', 'He|Ronghan|R|', 'Hu|Ying|Y|', 'Bai|Li|L|', 'Wang|Chunmei|C|', 'Wang|Kun|K|0000-0003-1396-6325', 'Zhu|Lei|L|0000-0002-4352-5013']
D017667:Adipocytes D000818:Animals D064592:Autografts D004195:Disease Models, Animal D008297:Male D051379:Mice D008807:Mice, Inbred BALB C D040901:Proteomics D012867:Skin D016038:Skin Transplantation D013234:Stem Cells D014182:Transplantation, Autologous D014184:Transplantation, Homologous D014945:Wound Healing
Microskin Adipose-derived stem cell Wound healing Full-thickness skin defect Secretome