Noninvasive In Vivo Fluorescence Imaging of NK Cells in Preclinical Models of Adoptive Immunotherapy.
Preclinical animal models play a vital role in developing novel adoptive immunotherapies for cancer. In these in vivo models, it is essential to track the adoptively transferred cells to understand their tissue localization (biodistribution) in order to correlate with observed therapeutic outcomes as well as to develop novel approaches to promote homing to tumors or organs of interest. This chapter describes a simple and quick method for fluorescence labeling and in vivo imaging of adoptively transferred NK cells in small animal models.
['Somanchi|Srinivas S|SS|']
D000818:Animals D045744:Cell Line, Tumor D002465:Cell Movement D004195:Disease Models, Animal D006801:Humans D016219:Immunotherapy, Adoptive D020014:K562 Cells D007694:Killer Cells, Natural D051379:Mice D061848:Optical Imaging D009928:Organ Specificity D014018:Tissue Distribution
Fluorescence imaging NK cells Adoptive immunotherapy DiR Near infrared dye