Rapid detection and differentiation of Staphylococcus colonies using an optical scattering technology.
Staphylococcus species are a major pathogen responsible for nosocomial infections and foodborne illnesses. We applied a laser-based BARDOT (bacterial rapid detection using optical scattering technology) for rapid colony screening and detection of Staphylococcus on an agar plate and differentiate these from non-Staphylococcus spp. Among the six growth media tested, phenol red mannitol agar (PRMA) was found most suitable for building the Staphylococcus species scatter image libraries. Scatter image library for Staphylococcus species gave a high positive predictive value (PPV 87.5-100%) when tested against known laboratory strains of Staphylococcus spp., while the PPV against non-Staphylococcus spp. was 0-38%. A total of nine naturally contaminated bovine raw milk and ready-to-eat chicken salad samples were tested, and BARDOT detected Staphylococcus including Staphylococcus aureus with 80-100% PPV. Forty-five BARDOT-identified bacterial isolates from naturally contaminated foods were further confirmed by tuf and nuc gene-specific PCR and 16S rRNA gene sequence. This label-free, non-invasive on-plate colony screening technology can be adopted by the food industries, biotechnology companies, and public health laboratories for Staphylococcus species detection including S. aureus from various samples for food safety and public health management. Graphical abstract.
['Alsulami|Tawfiq S|TS|', 'Zhu|Xingyue|X|', 'Abdelhaseib|Maha Usama|MU|', 'Singh|Atul K|AK|', 'Bhunia|Arun K|AK|']
D000818:Animals D015373:Bacterial Typing Techniques D002417:Cattle D002645:Chickens D000067493:Dynamic Light Scattering D005506:Food Contamination D005516:Food Microbiology D064088:Hazard Analysis and Critical Control Points D008460:Meat D008892:Milk D012336:RNA, Ribosomal, 16S D013203:Staphylococcal Infections D013210:Staphylococcus
Staphylococcus BARDOT 16S RNA gene Food sample Optical sensor