Quantitative tissue proteomic investigation of invasive ductal carcinoma of breast with luminal B HER2 positive and HER2 enriched subtypes towards potential diagnostic and therapeutic biomarkers.
Worldwide, breast cancer is one of the frequently diagnosed cancers in women with high mortality if not diagnosed at early stage. Although biomarker discoveries through various proteomic approaches have been studied in breast cancer, a limited number of studies have explored the invasive ductal carcinoma with Luminal B HER2 positive (LB) and HER2 enriched (HE) subtypes. The present study employed the complementary quantitative proteomic approaches to find a panel of markers that could discriminate LB and HE subtypes as well as early (ES) and late stages (LS) of these subtypes. A total of 67 and 68 differentially expressed proteins were identified by DIGE for the subtype and stage wise categories, respectively. Multivariate statistical analysis was employed to identify the set of most significant proteins, which could discriminate between these two subtypes and also early and late stages under study. Immunoblotting and MRM based validation in a separate cohort of samples confirmed that panel of biosignatures for LB are APOA1, GELS, HS90B, EF1A1, NHRF1 and PRDX3 and for HE are PRDX1, CATD, CALR, ATPB and CH60. For the diagnosis of early and late stages the potential markers are TPM4, CATD, PRDX3, ANXA3, HSPB1 and CALR, TRFE, GELS, CH60, CAPG, NHRF1, 1433G, GRP78 respectively.
['Pendharkar|Namita|N|', 'Gajbhiye|Akshada|A|', 'Taunk|Khushman|K|', 'RoyChoudhury|Sourav|S|', 'Dhali|Snigdha|S|', 'Seal|Shubhendu|S|', 'Mane|Anupama|A|', 'Abhang|Subodhini|S|', 'Santra|Manas K|MK|', 'Chaudhury|Koel|K|', 'Rapole|Srikanth|S|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D014408:Biomarkers, Tumor D001943:Breast Neoplasms D018270:Carcinoma, Ductal, Breast D000091342:Endoplasmic Reticulum Chaperone BiP D005260:Female D006801:Humans D008875:Middle Aged D058990:Molecular Targeted Therapy D009363:Neoplasm Proteins D020543:Proteome D018719:Receptor, ErbB-2 D015203:Reproducibility of Results D012680:Sensitivity and Specificity
Luminal B HER2 enriched Breast cancer Quantitative proteomics 2-D DIGE iTRAQ