Hydrogen sulfide prevents Abeta-induced neuronal apoptosis by attenuating mitochondrial translocation of PTEN.
Neuronal cell apoptosis is an important pathological change in Alzheimer's disease (AD). Hydrogen sulfide (H(2)S) is known to be a novel gaseous signaling molecule and a cytoprotectant in many diseases including AD. However, the molecular mechanism of the antiapoptosis activity of H(2)S in AD is not yet fully understood. The aim of the present study is to evaluate the inhibitory effects of H(2)S on Abeta (Aβ)-induced apoptosis and the molecular mechanisms underlying primary neuron cells. Our results showed that sodium hydrosulfide (NaHS), a donor of H(2)S, significantly ameliorated Aβ-induced cell apoptosis. NaHS also reversed the Aβ-induced translocation of the phosphatase and tensin homologs deleted on chromosome 10 (PTEN) from the cytosol to the mitochondria. Furthermore, H(2)S increased the level of p-AKT/AKT significantly. Interestingly, the antiapoptosis effects of H(2)S were blocked down by specific PI3K/AKT inhibitor wortmannin. In conclusion, these data indicate that H(2)S inhibits Aβ-induced neuronal apoptosis by attenuating mitochondrial translocation of PTEN and that activation of PI3K/AKT signaling pathway plays a critical role in H(2)S-mediated neuronal protection. Our findings provide a novel route into the molecular mechanisms of neuronal apoptosis in AD.
['Cui|Weigang|W|', 'Zhang|Yinghua|Y|', 'Yang|Chenxi|C|', 'Sun|Yiyuan|Y|', 'Zhang|Min|M|', 'Wang|Songtao|S|']
D000544:Alzheimer Disease D016229:Amyloid beta-Peptides D000730:Androstadienes D000818:Animals D017209:Apoptosis D006862:Hydrogen Sulfide D051379:Mice D008810:Mice, Inbred C57BL D008928:Mitochondria D009474:Neurons D018696:Neuroprotective Agents D051059:PTEN Phosphohydrolase D010446:Peptide Fragments D000081082:Phosphoinositide-3 Kinase Inhibitors D061251:Primary Cell Culture D051057:Proto-Oncogene Proteins c-akt D015398:Signal Transduction D013440:Sulfides D000077191:Wortmannin
hydrogen sulfide apoptosis PTEN Alzheimer’s disease PI3K