Engineering Hybrid Chemotaxis Receptors in Bacteria.
Most bacteria use transmembrane sensors to detect a wide range of environmental stimuli. A large class of such sensors are the chemotaxis receptors used by motile bacteria to follow environmental chemical gradients. In Escherichia coli, chemotaxis receptors are known to mediate highly sensitive responses to ligands, making them potentially useful for biosensory applications. However, with only four ligand-binding chemotaxis receptors, the natural ligand spectrum of E. coli is limited. The design of novel chemoreceptors to extend the sensing capabilities of E. coli is therefore a critical aspect of chemotaxis-based biosensor development. One path for novel sensor design is to harvest the large natural diversity of chemosensory functions found in bacteria by creating hybrids that have the signaling domain from E. coli chemotaxis receptors and sensory domains from other species. In this work, we demonstrate that the E. coli receptor Tar can be successfully combined with most typical sensory domains found in chemotaxis receptors and in evolutionary-related two-component histidine kinases. We show that such functional hybrids can be generated using several different fusion points. Our work further illustrates how hybrid receptors could be used to quantitatively characterize ligand specificity of chemotaxis receptors and histidine kinases using standardized assays in E. coli.
['Bi|Shuangyu|S|', 'Pollard|Abiola M|AM|', 'Yang|Yiling|Y|', 'Jin|Fan|F|', 'Sourjik|Victor|V|']
D057005:Bioengineering D015374:Biosensing Techniques D002633:Chemotaxis D004926:Escherichia coli D029968:Escherichia coli Proteins D008024:Ligands D011993:Recombinant Fusion Proteins D015398:Signal Transduction
chemotaxis ligand histidine kinase extracellular sensory domain hybrid chemoreceptors methyl-accepting chemotaxis protein