The over expression of long non-coding RNA ANRIL promotes epithelial-mesenchymal transition by activating the ATM-E2F1 signaling pathway in pancreatic cancer: An in vivo and in vitro study.
This study aims to investigate the roles of lncRNA ANRIL in epithelial-mesenchymal transition (EMT) by regulating the ATM-E2F1 signaling pathway in pancreatic cancer (PC). PC rat models were established and ANRIL overexpression and interference plasmids were transfected. The expression of ANRIL, EMT markers (E-cadherin, N-cadherin and Vimentin) and ATM-E2F1 signaling pathway-related proteins (ATM, E2F1, INK4A, INK4B and ARF) were detected. Small molecule drugs were applied to activate and inhibit the ATM-E2F1 signaling pathway. Transwell assay and the scratch test were adopted to detect cell invasion and migration abilities. ANRIL expression in the PC cells was higher than in normal pancreatic duct epithelial cells. In the PC rat models and PC cells, ANRIL interference promoted the expressions of INK4B, INK4A, ARF and E-cadherin, while reduced N-cadherin and Vimentin expression. Over-expressed ANRIL decreased the expression of INK4B, INK4A, ARF and E-cadherin, but raised N-cadherin and Vimentin expressions. By inhibiting the ATM-E2F1 signaling pathway in PC cells, E-cadherin expression increased but N-cadherin and Vimentin expressions decreased. After ANRIL was silenced or the ATM-E2F1 signaling pathway inhibited, PC cell migration and invasion abilities were decreased. In conclusion, over-expression of lncRNA ANRIL can promote EMT of PC cells by activating the ATM-E2F1 signaling pathway.
['Chen|Shi|S|', 'Zhang|Jia-Qiang|JQ|', 'Chen|Jiang-Zhi|JZ|', 'Chen|Hui-Xing|HX|', 'Qiu|Fu-Nan|FN|', 'Yan|Mao-Lin|ML|', 'Chen|Yan-Ling|YL|', 'Peng|Cheng-Hong|CH|', 'Tian|Yi-Feng|YF|', 'Wang|Yao-Dong|YD|']
D000818:Animals D064007:Ataxia Telangiectasia Mutated Proteins D045744:Cell Line, Tumor D002465:Cell Movement D050687:E2F1 Transcription Factor D058750:Epithelial-Mesenchymal Transition D015972:Gene Expression Regulation, Neoplastic D006801:Humans D009361:Neoplasm Invasiveness D010190:Pancreatic Neoplasms D034622:RNA Interference D062085:RNA, Long Noncoding D051381:Rats D015398:Signal Transduction
Long non-coding RNA ANRIL the ATM-E2F1 signaling pathway Pancreatic cancer Epithelial-mesenchymal transition and rat model