Methods to Study the Role of Progranulin in Preimplantation Mouse Embryo Development.
Progranulin is a 67-88 kDa glycoprotein, also known as acrogranin, PC-cell-derived growth factor, granulin-epithelin precursor, and proepithelin. This protein is present in a variety of mouse, rat, and human tissues. Progranulin, which is a growth factor, mediates cell cycle progression and cell migration in normal and pathological conditions. In several types of cancers, progranulin expression is upregulated, whereas function-interfering mutations in the granulin gene in humans have been linked to a subset of heritable cases of frontotemporal lobar degeneration. Also, progranulin has important effects on mouse preimplantation embryo development in vitro, including regulation of the appearance of the epithelium in the developing mouse blastocyst and growth of trophectoderm. Furthermore, progranulin promotes mouse blastocyst hatching, adhesion, and outgrowth in vitro. In this chapter, we describe some of the techniques that may be useful in the study of progranulin in embryo development.
['Diaz-Cueto|Laura|L|', 'Arechavaleta-Velasco|Fabian|F|', 'Gerton|George L|GL|']
D000818:Animals D001755:Blastocyst D001973:Bromodeoxyuridine D002448:Cell Adhesion D017077:Culture Media, Conditioned D004475:Ectoderm D004622:Embryo, Mammalian D047108:Embryonic Development D005260:Female D005455:Fluorescent Antibody Technique D008297:Male D051379:Mice D008967:Molecular Biology D000077153:Progranulins D012333:RNA, Messenger
Progranulin Embryo development