Hypoxia and Inflammation-Induced Disruptions of the Blood-Brain and Blood-Cerebrospinal Fluid Barriers Assessed Using a Novel T1-Based MRI Method.
Subtle blood-brain barrier (BBB) disruption is involved in numerous neurological conditions. This disruption is found diffusely in the brain and requires quantitative methods for assessment. We propose a statistical method to identify individual voxels where the BBB is disrupted using T1-weighted MRI. We used models of severe and focal vs. mild and generalized disruption of the BBB to show proof of principle with the cold injury model, hypoxia, and a model of inflammation using low- and high-dose lipopolysaccharide (LPS) treatment. Using voxel-based analysis, we found that mild hypoxia resulted in diffuse disruption of the BBB, whereas more severe hypoxia and high-dose LPS treatment resulted in prominent leakage, particularly in the periventricular area, suggestive of blood-cerebrospinal fluid (CSF) barrier disruption. Our data suggest that the periventricular area may be compromised first in conditions of inflammation and hypoxia. Voxel-based analysis could be used in future studies assessing subtle blood-CSF or BBB disruption.
['Nathoo|Nabeela|N|', 'Jalal|Hamza|H|', 'Natah|Sirajedin S|SS|', 'Zhang|Qiong|Q|', 'Wu|Ying|Y|', 'Dunn|Jeff F|JF|']
D000818:Animals D001812:Blood-Brain Barrier D001921:Brain D002555:Cerebrospinal Fluid D000067390:Cold Injury D003287:Contrast Media D004195:Disease Models, Animal D005682:Gadolinium D000860:Hypoxia D007249:Inflammation D008070:Lipopolysaccharides D008279:Magnetic Resonance Imaging D008297:Male D051381:Rats D017208:Rats, Wistar
Hypoxia Blood-cerebrospinal fluid barrier MRI Blood-brain barrier Inflammation Lipopolysaccharide Periventricular Sodium fluorescein