EPs7630(®) from Pelargonium sidoides increases stress resistance in Caenorhabditis elegans probably via the DAF-16/FOXO pathway.
EPs7630(®) a water alcohol extract of the roots from Pelargonium sidoides contains several secondary metabolites including highly oxygenated coumarins, various phenolics and polyphenols. Using the DPPH assay to measure antioxidant activity a free radical scavenging activity of 14.7±0.85μg/ml (IC50) was determined. As an in vivo model Caenorhabditis elegans was applied to study the effect of EPs7630(®) on stress resistance. EPs7630(®) treatment reduces intracellular hsp-16.2::GFP expression (induced by the pro-oxidant juglone) indicating that the secondary metabolites of EPs7630(®) are bioavailable and exhibit antioxidant activities in vivo. Application of EPs7630(®) (50μg/ml) to the transgenic mutant TJ356 induced the migration of the transcription factor DAF-16 from cytosol to the nucleus, suggesting a prominent role of DAF-16/FOXO in the daf-2 pathway for stress resistance.
['Rezaizadehnajafi|Leila|L|', 'Wink|Michael|M|']
D000818:Animals D017173:Caenorhabditis elegans D029742:Caenorhabditis elegans Proteins D051858:Forkhead Transcription Factors D009285:Naphthoquinones D018384:Oxidative Stress D031316:Pelargonium D010936:Plant Extracts D014157:Transcription Factors
Pelargonium sidoides Stress resistance Caenorhabditis elegans DAF-16