cfNOMe - A single assay for comprehensive epigenetic analyses of cell-free DNA.
Cell-free DNA (cfDNA) analysis has become essential in cancer diagnostics and prenatal testing. We present cfNOMe, a two-in-one method of measuring cfDNA cytosine methylation and nucleosome occupancy in a single assay using non-disruptive enzymatic cytosine conversion and a custom bioinformatic pipeline. We show that enzymatic cytosine conversion better preserves cfDNA fragmentation information than does bisulfite conversion. Whereas previously separate experiments were required to study either epigenetic marking, cfNOMe delivers reliable results for both, enabling more comprehensive and inexpensive epigenetic cfDNA profiling. cfNOMe has the potential to advance biomarker discovery and diagnostic usage in diseases with systemic perturbations of cfDNA composition.
['Erger|Florian|F|0000-0002-2768-1702', 'Nörling|Deborah|D|', 'Borchert|Domenica|D|', 'Leenen|Esther|E|', 'Habbig|Sandra|S|', 'Wiesener|Michael S|MS|', 'Bartram|Malte P|MP|', 'Wenzel|Andrea|A|', 'Becker|Christian|C|', 'Toliat|Mohammad R|MR|', 'Nürnberg|Peter|P|', 'Beck|Bodo B|BB|', 'Altmüller|Janine|J|']
D001681:Biological Assay D000073888:Cell-Free Nucleic Acids D019175:DNA Methylation D044127:Epigenesis, Genetic D057890:Epigenomics D006801:Humans D007674:Kidney Diseases D020641:Polymorphism, Single Nucleotide
cfNOMe Cell-free DNA Analysis software Methylome Nucleosome footprints