LncRNA MCF2L-AS1 aggravates the malignant development of colorectal cancer via targeting miR-105-5p/RAB22A axis.
BACKGROUND
Colorectal cancer (CRC) represents one of the major malignant cancers in the world. It has been demonstrated that long non-coding RNAs (lncRNAs) can cause great influences on various human cancers. Though MCF.2 cell line derived transforming sequence like antisense RNA 1 (MCF2L-AS1) and its carcinogenic effect in CRC has been elucidated by several previous researches, the underlying mechanism remains unknown.


AIM
We aimed at exploring the function and regulatory mechanism of MCF2L-AS1 in CRC.


METHODS
MCF2L-AS1 expression in CRC cells was tested via RT-qPCR assay. The effects of MCF2L-AS1 on the biological properties of CRC cells were testified through functional experiments. The molecular mechanism of MCF2L-AS1 was verified through mechanism experiments.


RESULTS
MCF2L-AS1 was highly expressed in CRC cells, and it could enhance the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) process of CRC cells. MiR-105-5p was sponged by MCF2L-AS1 in CRC cells and Ras-related protein Rab-22A (RAB22A) was verified to be the downstream target of miR-105-5p. It was verified through rescue assays that RAB22A overexpression or miR-105-5p silencing could reverse the repressive impact of MCF2L-AS1 silencing on CRC progression.


CONCLUSION
MCF2L-AS1 accelerated the malignant development of CRC cells by targeting the miR-105-5p/RAB22A axis.
['Kong|Wencheng|W|', 'Li|Hui|H|', 'Xie|Lesi|L|', 'Cui|Guangxing|G|', 'Gu|Weigang|W|', 'Zhang|Hongchen|H|', 'Ma|Wencong|W|', 'Zhou|Yifeng|Y|http://orcid.org/0000-0003-3665-907X']
D017209:Apoptosis D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D015179:Colorectal Neoplasms D018450:Disease Progression D058750:Epithelial-Mesenchymal Transition D020868:Gene Silencing D006801:Humans D035683:MicroRNAs D009361:Neoplasm Invasiveness D009363:Neoplasm Proteins D064067:Rho Guanine Nucleotide Exchange Factors D020691:rab GTP-Binding Proteins
MCF2L-AS1 Colorectal cancer miR-105-5p RAB22A