An immobilized liquid interface prevents device associated bacterial infection in vivo.
Virtually all biomaterials are susceptible to biofilm formation and, as a consequence, device-associated infection. The concept of an immobilized liquid surface, termed slippery liquid-infused porous surfaces (SLIPS), represents a new framework for creating a stable, dynamic, omniphobic surface that displays ultralow adhesion and limits bacterial biofilm formation. A widely used biomaterial in clinical care, expanded polytetrafluoroethylene (ePTFE), infused with various perfluorocarbon liquids generated SLIPS surfaces that exhibited a 99% reduction in S. aureus adhesion with preservation of macrophage viability, phagocytosis, and bactericidal function. Notably, SLIPS modification of ePTFE prevents device infection after S. aureus challenge in vivo, while eliciting a significantly attenuated innate immune response. SLIPS-modified implants also decrease macrophage inflammatory cytokine expression in vitro, which likely contributed to the presence of a thinner fibrous capsule in the absence of bacterial challenge. SLIPS is an easily implementable technology that provides a promising approach to substantially reduce the risk of device infection and associated patient morbidity, as well as health care costs.
['Chen|Jiaxuan|J|', 'Howell|Caitlin|C|', 'Haller|Carolyn A|CA|', 'Patel|Madhukar S|MS|', 'Ayala|Perla|P|', 'Moravec|Katherine A|KA|', 'Dai|Erbin|E|', 'Liu|Liying|L|', 'Sotiri|Irini|I|', 'Aizenberg|Michael|M|', 'Aizenberg|Joanna|J|', 'Chaikof|Elliot L|EL|']
D000818:Animals D001422:Bacterial Adhesion D001672:Biocompatible Materials D018441:Biofilms D002478:Cells, Cultured D005466:Fluorocarbons D006801:Humans D008297:Male D008810:Mice, Inbred C57BL D011138:Polytetrafluoroethylene D019736:Prostheses and Implants D013203:Staphylococcal Infections D013211:Staphylococcus aureus D013499:Surface Properties
Infection In vivo SLIPS Polytetrafluoroethylene Perfluorocarbon liquids Implant