Novel anti-nociceptive effects of cardamonin via blocking expression of cyclooxygenase-2 and transglutaminase-2.
Recently, we reported that Alpinia katsumadai (AK) has anti-nociceptive activity in vivo and that cardamonin (CDN) from AK suppresses the activity and expression of transglutaminase-2 (Tgase-2). However, it remains unknown whether CDN contributes to the anti-nociceptive activities of AK in vivo. We examined the anti-inflammatory effects of CDN in MG63 osteoblast-like cells and Raw264.7 macrophage-like cells treated with interleukin-1β treatment. CDN suppressed the expression of Tgase-2, cyclooxygenase-2 (COX-2), and p65 (nuclear factor-κB) in a concentration-dependent manner, and restored the expression of IκB in MG63 and Raw264.7 cells. However, CDN did not inhibit the activity of COX-2. Gene silencing of Tgase-2 reduced the COX-2 expression in MG63 cells. Phenylbenzoquinone (PBQ)-induced writhing, carrageenan-induced hyperalgesia, and rota-rod test were used to evaluate the anti-nociceptive activity in vivo. CDN (3-30 mg/kg, orally administered) significantly inhibited PBQ-induced writhing. CDN also produced a significant, dose-dependent increase in the withdrawal response latencies in carrageenan-induced hyperalgesia. The effects of CDN on PBQ-induced writhing were not caused by impaired motor functions. These results suggest that CDN might be helpful in controlling the pain from inflammatory diseases.
['Park|Mi Kyung|MK|', 'Lee|Hye Ja|HJ|', 'Choi|Jin Kyu|JK|', 'Kim|Hyun Ji|HJ|', 'Kang|June Hee|JH|', 'Lee|Eun Ji|EJ|', 'Kim|You Ri|YR|', 'Kang|Ju Hee|JH|', 'Yoo|Jung Ki|JK|', 'Cho|Hee Yeong|HY|', 'Kim|Jin Kyeoung|JK|', 'Kim|Chang-Hyun|CH|', 'Park|Jong Hwan|JH|', 'Lee|Chang Hoon|CH|']
D032429:Alpinia D000700:Analgesics D000818:Animals D016227:Benzoquinones D002351:Carrageenan D002460:Cell Line D047188:Chalcones D051546:Cyclooxygenase 2 D052246:Cyclooxygenase 2 Inhibitors D019204:GTP-Binding Proteins D015870:Gene Expression D006801:Humans D008297:Male D055670:Medicine, Korean Traditional D051379:Mice D008813:Mice, Inbred ICR D010146:Pain D010946:Plants, Medicinal D000091345:Protein Glutamine gamma Glutamyltransferase 2 D034741:RNA, Small Interfering D051381:Rats D017207:Rats, Sprague-Dawley D011503:Transglutaminases
Anti-nociceptive Cardamonin Transglutaminase-2 Alpinia katsumadai Phenylbenzoquinone Carrageenan-induced hyperalgesia