Determination of exogenous epigallocatechin gallate peracetate in mouse plasma using liquid chromatography with quadrupole time-of-flight mass spectrometry.
A robust method for the quantitation of epigallocatechin gallate peracetate in plasma for pharmacokinetic studies is lacking. We have developed a validated method to quantify this compound using liquid chromatography with quadrupole time-of-flight mass spectrometry with isopropanol and tert-butyl methyl ether (3:10) extraction and thin-layer chromatography purification. The epigallocatechin gallate peracetate-1-(13) C8 isotope was used as an internal standard. The linear range (r(2) > 0.9950) was from 0.05 to 100.00 μg/mL. The lower limit of quantification of the method was 0.05 μg/mL. Reproducibility, coefficient of variation, was between 0.7 and 12.6% (n = 6), accuracy between 83.7 and 104.6% (n = 5), and recovery ranged from 82.4 to 109.0% (n = 4). Ion suppression was approximately 40%. No mass spectral peaks were found to interfere between the standard and internal standard or the blank plasma extracts. Epigallocatechin gallate peracetate in plasma was stably stored at -80°C over three months even after three freeze-thaw cycles. Extracts were stable in the sampler at 4°C for over 48 h. Plasma levels were maintained at 1.36 μg/mL for 360 min after intraorbital intravenous injection at 50 mg/kg in mice. This method can be used to reliably measure epigallocatechin gallate peracetate in plasma for pharmacokinetic studies.
['Chu|Kai On|KO|', 'Man|Gene Chi Wai|GC|', 'Chan|Kwok Ping|KP|', 'Chu|Ching Yan|CY|', 'Chan|Tak Hang|TH|', 'Pang|Chi Pui|CP|', 'Wang|Chi Chiu|CC|']
D000818:Animals D002392:Catechin D002851:Chromatography, High Pressure Liquid D002855:Chromatography, Thin Layer D005260:Female D013058:Mass Spectrometry D051379:Mice D008813:Mice, Inbred ICR D010463:Peracetic Acid D010949:Plasma
Epigallocatechin gallate peracetate Liquid chromatography Mass spectrometry Validation Plasma profile