In vitro evaluation of random and aligned polycaprolactone/gelatin fibers via electrospinning for bone tissue engineering.
Scaffold, as an essential element of tissue engineering, should provide proper chemical and structural cues to direct tissue regeneration. In this study, aligned and random polycaprolactone (PCL)/gelatin fibrous scaffolds with different mass ratio were electrospun. Chemical, structural, and mechanical properties of PCL/gelatin fibrous scaffolds were characterized by FTIR and tensile measurements. The average diameters of different groups were between 334.96 ± 41.43 nm and 363.78 ± 50.49 nm. Blending PCL with gelatin increased the mechanical properties of the scaffolds. The cell culture results demonstrated that the mass ratio of PCL and gelatin showed no obvious effects on cell behavior, whereas the cell growth behavior was affected by the fibers orientation. Higher elongation ratio, enhanced cell proliferation and elevated alkaline phosphatase activity were observed for cells cultured on aligned fibers. The findings in our research provide insightful information for the design and fabrication of scaffolds for bone tissue engineering.
['Guo|Zhenzhao|Z|', 'Xu|Jiaming|J|', 'Ding|Shan|S|', 'Li|Hong|H|', 'Zhou|Changren|C|', 'Li|Lihua|L|']
D000818:Animals D001672:Biocompatible Materials D001842:Bone and Bones D002417:Cattle D002460:Cell Line D049109:Cell Proliferation D002470:Cell Survival D002478:Cells, Cultured D005780:Gelatin D008422:Materials Testing D051379:Mice D018613:Microscopy, Confocal D008855:Microscopy, Electron, Scanning D010006:Osteoblasts D011091:Polyesters D017550:Spectroscopy, Fourier Transform Infrared D013314:Stress, Mechanical D023822:Tissue Engineering D054457:Tissue Scaffolds
polycaprolactone gelatin electrospinning aligned fiber osteoblast