The Protective Effects of 5-Methoxytryptamine-α-lipoic Acid on Ionizing Radiation-Induced Hematopoietic Injury.
Antioxidants are prospective radioprotectors because of their ability to scavenge radiation-induced reactive oxygen species (ROS). The hematopoietic system is widely studied in radiation research because of its high radiosensitivity. In the present study, we describe the beneficial effects of 5-methoxytryptamine-α-lipoic acid (MLA), which was synthesized from melatonin and α-lipoic acid, against radiation-induced hematopoietic injury. MLA administration significantly enhanced the survival rate of mice after 7.2 Gy total body irradiation. The results showed that MLA not only markedly increased the numbers and clonogenic potential of hematopoietic cells but also decreased DNA damage, as determined by flow cytometric analysis of histone H2AX phosphorylation. In addition, MLA decreased the levels of ROS in hematopoietic cells by inhibiting NOX4 expression. These data demonstrate that MLA prevents radiation-induced hematopoietic syndrome by increasing the number and function of and by inhibiting DNA damage and ROS production in hematopoietic cells. These data suggest MLA is beneficial for the protection of radiation injuries.
['Li|Deguan|D|', 'Tian|Zhenyuan|Z|', 'Tang|Weisheng|W|', 'Zhang|Junling|J|', 'Lu|Lu|L|', 'Sun|Zhaojin|Z|', 'Zhou|Zewei|Z|', 'Fan|Feiyue|F|']
D008735:5-Methoxytryptamine D054508:Acute Radiation Syndrome D000818:Animals D004249:DNA Damage D006410:Hematopoiesis D006657:Histones D008297:Male D008550:Melatonin D051379:Mice D008810:Mice, Inbred C57BL D000074663:NADPH Oxidase 4 D019255:NADPH Oxidases D011839:Radiation, Ionizing D011837:Radiation-Protective Agents D017382:Reactive Oxygen Species D008063:Thioctic Acid
5-methoxytryptamine-α-lipoic acid radioprotectors reactive oxygen species (ROS) hematopoietic cells ionizing radiation radiation protection