Single-Cell RNA-Seq Analysis of Retinal Development Identifies NFI Factors as Regulating Mitotic Exit and Late-Born Cell Specification.
Precise temporal control of gene expression in neuronal progenitors is necessary for correct regulation of neurogenesis and cell fate specification. However, the cellular heterogeneity of the developing CNS has posed a major obstacle to identifying the gene regulatory networks that control these processes. To address this, we used single-cell RNA sequencing to profile ten developmental stages encompassing the full course of retinal neurogenesis. This allowed us to comprehensively characterize changes in gene expression that occur during initiation of neurogenesis, changes in developmental competence, and specification and differentiation of each major retinal cell type. We identify the NFI transcription factors (Nfia, Nfib, and Nfix) as selectively expressed in late retinal progenitor cells and show that they control bipolar interneuron and Müller glia cell fate specification and promote proliferative quiescence.
['Clark|Brian S|BS|', "Stein-O'Brien|Genevieve L|GL|", 'Shiau|Fion|F|', 'Cannon|Gabrielle H|GH|', 'Davis-Marcisak|Emily|E|', 'Sherman|Thomas|T|', 'Santiago|Clayton P|CP|', 'Hoang|Thanh V|TV|', 'Rajaii|Fatemeh|F|', 'James-Esposito|Rebecca E|RE|', 'Gronostajski|Richard M|RM|', 'Fertig|Elana J|EJ|', 'Goff|Loyal A|LA|', 'Blackshaw|Seth|S|']
D000818:Animals D049109:Cell Proliferation D063928:Ependymoglial Cells D018507:Gene Expression Regulation, Developmental D007395:Interneurons D051379:Mice D008938:Mitosis D051839:NFI Transcription Factors D058953:Neural Stem Cells D055495:Neurogenesis D000081246:RNA-Seq D012160:Retina D055351:Retinal Neurons D059010:Single-Cell Analysis
development neurogenesis cell fate Müller glia CoGAPS neural progenitor photoreceptor proliferation retina scRNA-seq single-cell RNA-sequencing