Rhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China.
Two Gram-stain-negative, rod-shaped bacterial strains (C5T and C16), isolated from root nodules of Phaseolus vulgaris L. in Jiangxi Province, PR China, were characterized by using a polyphasic taxonomical approach. The phylogenetic analysis of the 16S rRNA gene and three concatenated housekeeping genes (recA-glnII-atpD) revealed that C5T and C16 were members of the genus Rhizobium, yet were distinct from known species. The case for strain C5T representing a novel species was supported by genomic results. Pairwise digital DNA-DNA hybridization and average nucleotide identity values were much lower than the proposed and generally accepted species boundaries. The genome-based phylogenetic tree reconstructed by using the up-to-date bacterial core gene set consisting of 92 genes showed that the strains formed a monophyletic branch, further supporting this result. The symbiotic genes of nodC and nifH were identified in both strains; each could nodulate Phaseolus vulgaris and Glycine max but not Leucaena leucocephala, Pisum sativum or Medicago sativa plants. Major cellular fatty acids of C5T were summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c; 58.8 %), C18 : 1 ω7c 11-methyl (14.2 %) and C18 : 0 (8.1 %). The DNA G+C content of C5T was 61.4 mol%. Based on these genomic, chemotaxonomic and phenotypic characteristics, we propose a novel species: Rhizobium chutanense sp. nov. The type strain is C5T (=CCTCC AB 2018143T=LMG 30777T).
['Huo|Yunyun|Y|', 'Tong|Wenjun|W|', 'Wang|Juanjuan|J|', 'Wang|Fang|F|', 'Bai|Wenqing|W|', 'Wang|Entao|E|', 'Shi|Peng|P|', 'Chen|Weimin|W|', 'Wei|Gehong|G|']
D015373:Bacterial Typing Techniques D001482:Base Composition D002681:China D004269:DNA, Bacterial D005227:Fatty Acids D005798:Genes, Bacterial D000455:Medicago sativa D009693:Nucleic Acid Hybridization D018532:Peas D027805:Phaseolus D010802:Phylogeny D012336:RNA, Ribosomal, 16S D012231:Rhizobium D053204:Root Nodules, Plant D017422:Sequence Analysis, DNA D013025:Soybeans D013559:Symbiosis
rhizobium Phaseolus vulgaris L. 16S rRNA ANI dDDH