Commercial Honeybush (Cyclopia spp.) Tea Extract Inhibits Osteoclast Formation and Bone Resorption in RAW264.7 Murine Macrophages-An in vitro Study.
Honeybush tea, a sweet tasting caffeine-free tea that is indigenous to South Africa, is rich in bioactive compounds that may have beneficial health effects. Bone remodeling is a physiological process that involves the synthesis of bone matrix by osteoblasts and resorption of bone by osteoclasts. When resorption exceeds formation, bone remodeling can be disrupted resulting in bone diseases such as osteoporosis. Osteoclasts are multinucleated cells derived from hematopoietic precursors of monocytic lineage. These precursors fuse and differentiate into mature osteoclasts in the presence of receptor activator of NF-kB ligand (RANKL), produced by osteoblasts. In this study, the in vitro effects of an aqueous extract of fermented honeybush tea were examined on osteoclast formation and bone resorption in RAW264.7 murine macrophages. We found that commercial honeybush tea extract inhibited osteoclast formation and TRAP activity which was accompanied by reduced bone resorption and disruption of characteristic cytoskeletal elements of mature osteoclasts without cytotoxicity. Furthermore, honeybush tea extract decreased expression of key osteoclast specific genes, matrix metalloproteinase-9 (MMP-9), tartrate resistant acid phosphatase (TRAP) and cathepsin K. This study demonstrates for the first time that honeybush tea may have potential anti-osteoclastogenic effects and therefore should be further explored for its beneficial effects on bone.
['Visagie|Amcois|A|', 'Kasonga|Abe|A|', 'Deepak|Vishwa|V|', 'Moosa|Shaakirah|S|', 'Marais|Sumari|S|', 'Kruger|Marlena C|MC|', 'Coetzee|Magdalena|M|']
D000135:Acid Phosphatase D000199:Actins D000818:Animals D001862:Bone Resorption D056657:Cathepsin K D002454:Cell Differentiation D002460:Cell Line D002470:Cell Survival D002851:Chromatography, High Pressure Liquid D004353:Drug Evaluation, Preclinical D016142:Holoprosencephaly D007527:Isoenzymes D008264:Macrophages D020780:Matrix Metalloproteinase 9 D051379:Mice D010010:Osteoclasts D008517:Phytotherapy D010936:Plant Extracts D053245:RANK Ligand D000067996:RAW 264.7 Cells D013019:South Africa D000071681:Tartrate-Resistant Acid Phosphatase D013662:Tea
osteoclast bone resorption honeybush tea RANKL RAW264.7  murine macrophages