Resveratrol supplementation improves DNA integrity and sperm parameters in streptozotocin-nicotinamide-induced type 2 diabetic rats.
Reproductive dysfunction is one of the diabetes complications. Resveratrol, a polyphenol compound, shows antidiabetic and antioxidant effects. The aim of the present study was to investigate the protective effects of resveratrol on sperm parameters and chromatin quality in experimentally induced type 2 diabetes by streptozotocin and nicotinamide. Forty male adult Wistar rats were grouped into normal control, diabetic control and resveratrol-treated diabetic groups (1, 5 and 10 mg/kg orally treated for 30 days). Type 2 diabetes was induced using a single dose of streptozotocin and nicotinamide by intraperitoneal injection. Then, the different parameters and chromatin condensation of the epididymal extracted spermatozoon were studied using aniline blue (AB), acridine orange (AO) and toluidine blue (TB) staining. The sperm parameters including count, motility and viability had significant reduction in diabetic rats (p < 0.05). Resveratrol increased count, motility and viable spermatozoa relative to the diabetic group (p < 0.05). The mean percentage of AB, AO and TB staining positive spermatozoa was increased in diabetic groups compared to control (p < 0.001) and decreased after treatment with 1 and 5 mg/kg resveratrol (p < 0.001). The results of AO and TB staining showed that resveratrol did not have any beneficial effect on chromatin condensation and denatured DNA at the dose of 10 mg/kg.
['Bahmanzadeh|Maryam|M|https://orcid.org/0000-0002-6585-9444', 'Goodarzi|Mohammad Taghi|MT|https://orcid.org/0000-0002-5546-5812', 'Rezaei Farimani|Azam|A|https://orcid.org/0000-0002-7853-2993', 'Fathi|Nasibeh|N|https://orcid.org/0000-0002-5825-4092', 'Alizadeh|Zohreh|Z|https://orcid.org/0000-0003-4490-3204']
D000818:Animals D000975:Antioxidants D002843:Chromatin D042002:Chromatin Assembly and Disassembly D004247:DNA D053938:DNA Fragmentation D003921:Diabetes Mellitus, Experimental D003924:Diabetes Mellitus, Type 2 D019587:Dietary Supplements D004305:Dose-Response Relationship, Drug D006801:Humans D007248:Infertility, Male D008297:Male D009536:Niacinamide D051381:Rats D017208:Rats, Wistar D000077185:Resveratrol D013076:Sperm Count D013081:Sperm Motility D013094:Spermatozoa D013311:Streptozocin D016896:Treatment Outcome
resveratrol antioxidants chromatin spermatozoon diabetes mellitus hyperglycaemia male reproductive system