Multigenerational analysis of the functional status of male reproductive system in mice after exposure to realistic doses of manganese.
The present study aimed to analyze the effect of multigenerational exposure to Mn at realistic doses on the functional quality of the male reproductive system of mice. Females and males (generation F0) were treated for 60 days with MnCl2, via gavage, at the doses of 0, 0.013, 0.13, and 1.3 mg/kg/day. Treatment of F0 dams continued throughout gestation and lactation periods. At the time of weaning, the offspring (F1 generation) was divided into: animals that were not exposed after weaning - parental exposure (PE); and those exposed via parental generation and directly (PDE) for additional 60 days, at the same dose of F0 generation. F0 and F1 males were euthanized for assessment of sperm parameters and redox changes in the reproductive system. There was a decrease in the sperm concentration of the F0 generation. In addition, the sperm parameters of F1 generation were drastically affected. The activity of antioxidant enzymes was significantly reduced in PE animals. It was possible to verify that the biochemical damages were higher in the PE individuals, as demonstrated by the integrated biomarker response index. Our results show that Mn, even at low doses, is able to promote a reduction in sperm quality over a generation.
['Souza|Tugstênio L|TL|', 'Batschauer|Amândia R|AR|', 'Brito|Patricia Manuitt|PM|', 'Oliveira Ribeiro|Ciro A|CA|', 'Martino-Andrade|Anderson J|AJ|', 'Ortolani-Machado|Claudia F|CF|']
D000818:Animals D015415:Biomarkers D002374:Catalase D004785:Environmental Pollutants D005260:Female D005837:Genitalia, Male D005982:Glutathione Transferase D008297:Male D008345:Manganese D051379:Mice D009929:Organ Size D011247:Pregnancy D012098:Reproduction D013081:Sperm Motility D013094:Spermatozoa D013482:Superoxide Dismutase
Developmental toxicology Oxidative stress Reproductive toxicology Sperm analysis