Well-designed dopamine-imprinted polymer interface for selective and quantitative dopamine detection among catecholamines using a potentiometric biosensor.
We report a well-designed biointerface enabling the selective and quantitative detection of dopamine (DA) using a potentiometric biosensor. To enhance the detection selectivity of DA, a DA-templated molecularly imprinted polymer (DA-MIP) was synthesized on the extended Au gate electrode of a field-effect transistor (FET) biosensor. For a quantitative DA analysis, the thickness of the DA-MIP was controlled to ca. 60 nm by surface-initiated atom transfer radical polymerization (SI-ATRP). In this process, the DA-MIP was copolymerized with vinylphenylboronic acid (vinyl-PBA), inducing molecular charges at the biointerface of the FET gate electrode. These charges were generated by the diol-binding between PBA and dopamine (a catecholamine), and were directly detected as a change in surface potential. In fact, the surface potential at the gate of the DA-MIP-coated FET responded significantly to DA added at concentrations ranging from 40 nM to 20 μM, whereas that of a non-imprinted polymer (NIP)-coated FET hardly changed over this range. Moreover, by measuring the kinetic parameters and electrochemical properties of well-designed devices with various added catecholamines, we confirmed that the DA-MIP-coated FET biosensor selectively and quantitatively detects DA.
['Kajisa|Taira|T|', 'Li|Wei|W|', 'Michinobu|Tsuyoshi|T|', 'Sakata|Toshiya|T|']
D015374:Biosensing Techniques D002395:Catecholamines D004298:Dopamine D055664:Electrochemical Techniques D004867:Equipment Design D006046:Gold D054802:Molecular Imprinting D058105:Polymerization D011108:Polymers D013499:Surface Properties D013816:Thermodynamics
Dopamine Catecholamine Molecularly imprinted polymer Field-effect transistor Surface-initiated atom transfer radical polymerization