Optimization of the droplet electroporation method for wild type Chlamydomonas reinhardtii transformation.
We performed the transformation of a wild type Chlamydomonas reinhardtii by optimizing previously developed droplet EP method. For more effective and faster optimization, we used DNA dying fluorescent molecule (Yo-Pro-1) for finding optimal EP conditions instead of using protein expression based evaluation method. By examining wider range of electrical parameter space together with the analysis of total current flow of EP process, we found optimal EP conditions. The obtained optimal EP conditions were verified by CFP transgene expression experiments. By applying the optimal EP conditions to the transformation of C. reinhardtii, we obtained transformants and analyzed them using PCR. Finally, implications and future work are discussed.
['Kim|Yeong Hun|YH|', 'Kwon|Sang Gu|SG|', 'Bae|Seo Jun|SJ|', 'Park|Sung Jin|SJ|', 'Im|Do Jin|DJ|']
D001583:Benzoxazoles D016825:Chlamydomonas reinhardtii D004247:DNA D018274:Electroporation D004867:Equipment Design D005456:Fluorescent Dyes D015870:Gene Expression D011806:Quinolinium Compounds D014170:Transformation, Genetic D019076:Transgenes
Optimization Droplet Electroporation Chlamydomonas reinhardtii Transformation