Intestinal absorption of lysozyme, an egg-white allergen, in rats: kinetics and effect of NSAIDs.
The absorption pathway(s) of a representative food allergen, lysozyme, and the mechanisms of lysozyme absorption facilitated by non-steroidal anti-inflammatory drugs were examined by intestinal closed-loop and re-circulating perfusion methods in rats. The absorption rate of fluorescein isothiocyanate (FITC)-labeled lysozyme in the proximal intestine was higher than that for a marker of non-specific absorption, FD-10, and was suppressed by colchicine (endocytosis inhibitor). Aspirin increased the absorption of FITC-lysozyme in the proximal intestine with no effects on tissue accumulation. Diclofenac facilitated FITC-lysozyme absorption, but meloxicam and loxoprofen exerted no effects on absorption. Co-administration of misoprostol (synthetic prostaglandin-E1 analog) with aspirin significantly ameliorated the aspirin-facilitated absorption of FITC-lysozyme to the same level as that seen with controls. Thus, lysozyme absorption was mediated by endocytic and paracellular pathways in the proximal intestine, and was facilitated by aspirin and diclofenac after impairment of the paracellular pathway. Misoprostol may suppress the allergen absorption facilitated by aspirin.
['Yokooji|Tomoharu|T|', 'Hamura|Koh|K|', 'Matsuo|Hiroaki|H|']
D000485:Allergens D000818:Animals D000894:Anti-Inflammatory Agents, Non-Steroidal D001241:Aspirin D004008:Diclofenac D004347:Drug Interactions D004527:Egg Proteins D007408:Intestinal Absorption D008297:Male D008657:Metabolic Clearance Rate D016595:Misoprostol D009113:Muramidase D051381:Rats D017207:Rats, Sprague-Dawley
Absorption Lysozyme NSAIDs Non-steroidal anti-inflammatory drugs Non-steroidal anti-inflammatory drugs fluorescein isothiocyanate Endocytosis Paracellular AUC CBB COX DMSO FD FDEIA FITC Ig PBS SDS–PAGE area under the plasma concentration–time curve coomassie brilliant blue cyclooxygenase dimethyl sulfoxide fluorescein isothiocyanate-labeled dextran food-dependent exercise-induced anaphylaxis immunoglobulin non-steroidal anti-inflammatory drugs phosphate-buffered saline sodium dodecyl sulfate–polyacrylamide gel electrophoresis