Physicochemical characterization of mineral deposits in human ligamenta flava.
The aim of our study was the detailed characterization of calcium deposits in ligamenta flava. The use of microcomputed tomography allowed extending the routine medical investigations to characterize mineral grains in the microscopic scale. A possible connection between spinal stenosis and ligament mineralization was investigated. The studies were carried out on 24 surgically removed ligamentum flavum samples divided into control and stenosis groups. Physicochemical characterization of the inorganic material was performed using X-ray fluorescence, X-ray diffraction, and Fourier transform infrared spectroscopy. The minerals were present in 14 of 24 ligament samples, both in stenosis and control groups. The inorganic substance constitutes on average ~0.1% of the sample volume. The minerals are scattered in the soft tissue matrix without any regular pattern. It was confirmed that minerals possess an internal structure and consist of the organic material and small inorganic grains mixture. The physicochemical analyses show that the predominant crystalline phase was hydroxyapatite (HAP). In the stenosis group calcium pyrophosphate dehydrate (CPPD) was identified. Both structures were never present in a single sample. Two different crystal structures suggest two independent processes of mineralization. The formation of CPPD may be treated as a more intense process since CPPD minerals are characterized by bigger values of the structural parameters and higher density than HAP deposits. The formation of HAP minerals is a soft tissue degeneration process that begins, in some cases, at early age or may not occur at all. Various density and volume of mineral grains indicate that the mineralization process does not occur in a constant environment and proceeds with various speeds. The formation of minerals in ligamenta flava is not directly associated with diagnosed spinal canal stenosis.
['Orzechowska|Sylwia|S|', 'Wróbel|Andrzej|A|', 'Kozieł|Marcin|M|', 'Łasocha|Wiesław|W|', 'Rokita|Eugeniusz|E|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D015519:Bone Density D055598:Chemical Phenomena D017886:Durapatite D005260:Female D006801:Humans D017843:Ligamentum Flavum D008297:Male D008875:Middle Aged D008903:Minerals D013052:Spectrometry, X-Ray Emission D017550:Spectroscopy, Fourier Transform Infrared D014961:X-Ray Diffraction D055114:X-Ray Microtomography D055815:Young Adult
Physicochemical characterization Mineralization Ligamentum flavum Micro-tomography Structural analysis