Stabilization of chitosan-based polyelectrolyte nanoparticle cargo delivery biomaterials by a multiple ionic cross-linking strategy.
PolyElectrolyte Nanoparticles (PENs) obtained by layer-by-layer self-assembly of polycations/polyanions suffer from a lack of colloidal stability in physiological conditions. We report a simple innovative approach for increasing their stability by multiple ionic cross-linkers. Herein, a chitosan-based core was stabilized by polyanions such as tripolyphosphate and dextran sulfate at pHs of 3 (aPENs) and 8 (bPENs) to improve the quality of electrostatic interactions in the core and manage self-assembly of polyethyleneimine shell onto the core. The physicochemical properties of the particles were characterized by DLS, SEM, TEM, FT-IR, and TGA. TEM micrographs showed visible core/shell structures of bPENs. From particle size and polydispersity indices, the bPENs stability was salt concentration-dependent. The release profiles of PENs using nicotinic acid demonstrated sustained release in a pH-independent manner with a good fit of Korsmeyer-Peppas model. These results suggest that multiple ionic cross-linkers can be an efficient approach to increase the colloidal stability of PENs.
['Motiei|Marjan|M|', 'Sedlařík|Vladimír|V|', 'Lucia|Lucian A|LA|', 'Fei|Haojie|H|', 'Münster|Lukáš|L|']
D001672:Biocompatible Materials D048271:Chitosan D003432:Cross-Linking Reagents D016264:Dextran Sulfate D016503:Drug Delivery Systems D006801:Humans D006863:Hydrogen-Ion Concentration D007477:Ions D053758:Nanoparticles D010316:Particle Size D000071228:Polyelectrolytes D017550:Spectroscopy, Fourier Transform Infrared
Polyelectrolyte nanoparticles Colloidal stability Tripolyphosphate Dextran sulfate Polyethyleneimine Chitosan