Magnitude of influenza virus replication and cell damage is associated with interleukin-6 production in primary cultures of human tracheal epithelium.
Primary cultures of human tracheal epithelium were infected with influenza viruses to examine the relationships between the magnitude of viral replication and infection-induced cell damage and cytokine production in airway epithelial cells. Infection with four strains of the type A influenza virus increased the detached cell number and lactate dehydrogenase (LDH) levels in the supernatants. The detached cell number and LDH levels were related to the viral titers and interleukin (IL)-6 levels and the nuclear factor kappa B (NF-κB) p65 activation. Treatment of the cells with an anti-IL-6 receptor antibody and an NF-κB inhibitor, caffeic acid phenethyl ester, reduced the detached cell number, viral titers and the LDH levels and improved cell viability after infection with the pandemic influenza virus [A/Sendai-H/N0633/2009 (H1N1) pdm09]. A caspase-3 inhibitor, benzyloxycarbonyl-DEVD-fluoromethyl ketone, reduced the detached cell number and viral titers. Influenza viral infection-induced cell damage may be partly related to the magnitude of viral replication, NF-κB-p65-mediated IL-6 production and caspase-3 activation.
['Yamaya|Mutsuo|M|', 'Nadine|Lusamba K|LK|', 'Ota|Chiharu|C|', 'Kubo|Hiroshi|H|', 'Makiguchi|Tomonori|T|', 'Nagatomi|Ryoichi|R|', 'Nishimura|Hidekazu|H|']
D000368:Aged D000369:Aged, 80 and over D000403:Airway Resistance D000704:Analysis of Variance D000818:Animals D000906:Antibodies D002415:Cats D002478:Cells, Cultured D003374:Coumarins D004791:Enzyme Inhibitors D004847:Epithelial Cells D005260:Female D015967:Gene Expression Regulation, Viral D006801:Humans D009980:Influenza A virus D015850:Interleukin-6 D007770:L-Lactate Dehydrogenase D008297:Male D009842:Oligopeptides D012333:RNA, Messenger D013997:Time Factors D014132:Trachea D051996:Transcription Factor RelA D014162:Transfection D014764:Viral Proteins D014779:Virus Replication
Influenza virus Cell damage Interleukin-6 Airway epithelial cells Pathogenicity