Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 Å resolution.
Here, a 1.82 Å resolution X-ray structure of a glycoside hydrolase family 74 (GH74) enzyme from Acidothermus cellulolyticus is reported. The resulting structure was refined to an R factor of 0.150 and an Rfree of 0.196. Structural analysis shows that five related structures have been reported with a secondary-structure similarity of between 75 and 89%. The five similar structures were all either Clostridium thermocellum or Geotrichum sp. M128 GH74 xyloglucanases. Structural analysis indicates that the A. cellulolyticus GH74 enzyme is an endoxyloglucanase, as it lacks a characteristic loop that blocks one end of the active site in exoxyloglucanases. Superimposition with the C. thermocellum GH74 shows that Asp451 and Asp38 are the catalytic residues.
['Alahuhta|Markus|M|', 'Adney|William S|WS|', 'Himmel|Michael E|ME|', 'Lunin|Vladimir V|VV|']
D000192:Actinomycetales D000595:Amino Acid Sequence D001426:Bacterial Proteins D048013:Clostridium thermocellum D018360:Crystallography, X-Ray D004926:Escherichia coli D015870:Gene Expression D005848:Geotrichum D006026:Glycoside Hydrolases D008958:Models, Molecular D008969:Molecular Sequence Data D054730:Protein Interaction Domains and Motifs D017433:Protein Structure, Secondary D011994:Recombinant Proteins D016415:Sequence Alignment D040681:Structural Homology, Protein
glycoside hydrolases GH74 xyloglucanases endoglucanases