Strong Plasmonic Enhancement of a Single Peridinin-Chlorophyll a-Protein Complex on DNA Origami-Based Optical Antennas.
In this contribution, we fabricate hybrid constructs based on a natural light-harvesting complex, peridinin-chlorophyll a-protein, coupled to dimer optical antennas self-assembled with the help of the DNA origami technique. This approach enables controlled positioning of individual complexes at the hotspot of the optical antennas based on large, colloidal gold and silver nanoparticles. Our approach allows us to selectively excite the different pigments present in the harvesting complex, reaching a fluorescence enhancement of 500-fold. This work expands the range of self-assembled functional hybrid constructs for harvesting sunlight and can be further developed for other pigment-proteins and proteins.
['Kaminska|Izabela|I|0000-0002-9435-8198', 'Bohlen|Johann|J|', 'Mackowski|Sebastian|S|', 'Tinnefeld|Philip|P|0000-0003-4290-7770', 'Acuna|Guillermo P|GP|0000-0001-8066-2677']
D002338:Carotenoids D000077194:Chlorophyll A D004247:DNA D004141:Dinoflagellida D005453:Fluorescence D017979:Gold Colloid D045342:Light-Harvesting Protein Complexes D053768:Metal Nanoparticles D008958:Models, Molecular D049329:Nanostructures D015800:Protozoan Proteins D012834:Silver D013472:Sunlight
plasmonics optical antenna DNA origami fluorescence enhancement photosynthetic complex single-molecule detection