Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection
Introduction: The World Health Organization has categorized Helicobacter pylori as a carcinogen for gastric
cancer, which causes human mortality worldwide. A number of studies have shown that H. pylori affects cell signaling
in gastric epithelial cells and changes the expression of some proteins such as proinflammatory cytokines. Bacterial
infections may alter sirt1 and sirt2 genes expression in inflammatory tissues and cancer cells. In this study, sirt1 and
sirt2 genes expression in gastric cancers was surveyed with reference to H. pylori status. Methods: Stomach biopsies
were collected from 50 gastric cancer patients, 25 H. pylori-positive and 25 H. pylori-negative as determined by the
urea rapid test. Tumor grade was determined by a pathologist. After total RNA extraction from gastric cancer biopsy
samples and cDNA synthesis, sirt1 and sirt2 genes expression levels were determined by Real Time PCR and ΔΔCT
methods. Results: There was no statistically significant link between H. pylori infection and sirt1 (P<0.899) and sirt2
(P<0.169) genes expression in gastric epithelial cells. However, pathologic findings showed that there is a statistically
significant relationship between sirt1 gene expression and the tumor grade (P<0.024). Discussion: A statistically
significant association was found between sirt1 gene expression and tumor grade of gastric cancers that could be due
to effects on progression of cancer cells infected with H. pylori.
['Mohammadi Saravle|Saman|S|', 'Ahmadi Hedayati|Manouchehr|M|', 'Mohammadi|Ebrahim|E|', 'Sheikhesmaeili|Farshad|F|', 'Nikkhou|Bahram|B|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D014408:Biomarkers, Tumor D016022:Case-Control Studies D004847:Epithelial Cells D005260:Female D005500:Follow-Up Studies D005753:Gastric Mucosa D016481:Helicobacter Infections D016480:Helicobacter pylori D006801:Humans D008297:Male D008875:Middle Aged D009367:Neoplasm Staging D056564:Sirtuin 1 D013270:Stomach D013274:Stomach Neoplasms
sirt1 tumor stages Helicobacter pylori sirt2 gastric cancer