Proteomic Analysis of Rat Hippocampus for Studies of Cognition and Memory Loss with Aging.
This chapter describes a protocol for proteomic profiling of the rat hippocampal proteome using a combination of liquid chromatography tandem mass spectrometry (LC-MS/MS) and data analysis to determine the cellular location of the identified proteins. In the example given, many of these proteins were localized in the plasma membrane and nucleus. These could be of interest in further studies of neurological and neurodegenerative disorders linked with hippocampal dysfunction, such as aging, major depression, and Alzheimer's disease.
['Guest|Paul C|PC|', 'Rahmoune|Hassan|H|', 'Martins-de-Souza|Daniel|D|']
D000375:Aging D000544:Alzheimer Disease D000818:Animals D002853:Chromatography, Liquid D003071:Cognition D006624:Hippocampus D008569:Memory Disorders D020543:Proteome D040901:Proteomics D051381:Rats D017207:Rats, Sprague-Dawley D053719:Tandem Mass Spectrometry
Proteome Hippocampus Cognition Memory Mass spectrometry Major depression Alzheimer’s disease