The inhibition of polyamine biosynthesis weakens the drought tolerance in white clover (Trifolium repens) associated with the alteration of extensive proteins.
Changes of endogenous polyamine (PA) levels could be a key adaptive response to drought in plants. White clover pretreated with or without dicyclohexylamine (DCHA), an inhibitor of PA biosynthesis, was subjected to drought stress induced by 18% polyethylene glycol 6000 for 8 days in controlled growth chambers. Results showed that drought stress significantly increased endogenous PA content, whereas DCHA significantly decreased PA accumulation under drought stress. The attenuate PA biosynthesis was unfavorable for plant growth and drought tolerance, as reflected by significantly lower relative water content, relative growth rate, instantaneous water use efficiency, and cell membrane stability in leaves in response to drought. On the basis of proteomic analysis, the inhibition of PA synthesis decreased the accumulation of many key differentially expressed proteins including (1) ribosomal structure and biogenesis: elongation factor, ribosomal protein S10E, and 30S ribosomal protein; (2) amino acid transport and metabolism: cysteine synthase, delta-1-pyrroline-5-carboxylate synthetase, and glutamate decarboxylase; (3) carbohydrate metabolism and energy production: photosystem apoprotein, sucrose-phosphate synthase, phosphogluconate dehydrogenase, sucrose-phosphatase, NADH oxidoreductase, and ATP synthase; (4) antioxidant metabolism: catalase, peroxidase I, ascorbate peroxidase, and glutathione S-transferase; and (5) other biological processes: heat shock protein 70, heat shock protein 90, and calcium-dependent protein kinase associated with the decreased drought tolerance in white clover. These findings indicate that PAs play a critical role in the regulation of growth, ribosome, amino acid and energy metabolism, and antioxidant reactions in white clover under drought stress. Drought-induced increases in endogenous PAs could be one of key adaptive responses against drought stress in white clover.
['Li|Zhou|Z|', 'Zhang|Yan|Y|', 'Peng|Dandan|D|', 'Peng|Yan|Y|http://orcid.org/0000-0001-7776-877X', 'Zhang|Xinquan|X|', 'Ma|Xiao|X|', 'Huang|Linkai|L|', 'Yan|Yanhong|Y|']
D053898:Biosynthetic Pathways D016000:Cluster Analysis D055864:Droughts D018506:Gene Expression Regulation, Plant D063990:Gene Ontology D007553:Isotope Labeling D018515:Plant Leaves D010940:Plant Proteins D011073:Polyamines D060066:Protein Interaction Maps D012333:RNA, Messenger D013312:Stress, Physiological D029921:Trifolium
Growth Differentially expressed proteins Metabolic pathway Photosynthesis iTRAQ-based proteome