Assessment of CD27 expression as a tool for active and latent tuberculosis diagnosis.
UNLABELLED
There are still no reliable tests to distinguish active tuberculosis (TB) from latent TB infection (LTBI). Assessment of CD27 modulation on CD4⁺ T-cells has been suggested as a tool to diagnose different TB stages.


OBJECTIVES
To use several cytometric approaches to evaluate CD27 expression on Mycobacterium tuberculosis (Mtb)-specific CD4⁺ T-cells to differentiate TB stages.


METHODS
55 HIV-uninfected subjects were enrolled: 13 active TB; 12 cured TB; 30 LTBI. Whole blood was stimulated with RD1-proteins or Cytomegalovirus-lysate (CMV). Interferon (IFN)-γ response was evaluated by cytometry. The proportion of CD27(±) within the IFN-γ⁺ CD4⁺ T-cells or RATIO of the CD27-median fluorescence intensity (MFI) of CD4⁺ T-cells over the CD27 MFI of IFN-γ⁺ CD4⁺ T-cells was evaluated.


RESULTS
The greatest diagnostic accuracy in discriminating active TB vs. LTBI or cured TB was reached by evaluating the CD27(+) CD45RA(-) cells within the IFN-γ⁺ CD4⁺ T-cell subset (76.92 sensitivity for both, and 90% and 91.67% specificity, respectively), although the use of the CD27 MFI RATIO allows for stricter data analysis, independent of the operator.


CONCLUSIONS
the study of CD27 expression using different approaches, whether it involves evaluation of CD45RA expression or not, is a robust biomarker for discriminating TB stages.
['Petruccioli|Elisa|E|', 'Petrone|Linda|L|', 'Vanini|Valentina|V|', 'Cuzzi|Gilda|G|', 'Navarra|Assunta|A|', 'Gualano|Gina|G|', 'Palmieri|Fabrizio|F|', 'Girardi|Enrico|E|', 'Goletti|Delia|D|']
D000328:Adult D000942:Antigens, Bacterial D015415:Biomarkers D015496:CD4-Positive T-Lymphocytes D005260:Female D005434:Flow Cytometry D006801:Humans D007371:Interferon-gamma D055985:Latent Tuberculosis D017493:Leukocyte Common Antigens D008297:Male D008875:Middle Aged D009169:Mycobacterium tuberculosis D012680:Sensitivity and Specificity D014376:Tuberculosis D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 D055815:Young Adult
CD27 Tuberculosis Diagnosis LTBI Biomarker