Engineering Rhodosporidium toruloides for increased lipid production.
Oleaginous yeast are promising organisms for the production of lipid-based chemicals and fuels from simple sugars. In this work, we explored Rhodosporidium toruloides for the production of lipid-based products. This oleaginous yeast natively produces lipids at high titers and can grow on glucose and xylose. As a first step, we sequenced the genomes of two strains, IFO0880, and IFO0559, and generated draft assemblies and annotations. We then used this information to engineer two R. toruloides strains for increased lipid production by over-expressing the native acetyl-CoA carboxylase and diacylglycerol acyltransferase genes using Agrobacterium tumefaciens mediated transformation. Our best strain, derived from IFO0880, was able to produce 16.4 ± 1.1 g/L lipid from 70 g/L glucose and 9.5 ± 1.3 g/L lipid from 70 g/L xylose in shake-flask experiments. This work represents one of the first examples of metabolic engineering in R. toruloides and establishes this yeast as a new platform for production of fatty-acid derived products.
['Zhang|Shuyan|S|', 'Skerker|Jeffrey M|JM|', 'Rutter|Charles D|CD|', 'Maurer|Matthew J|MJ|', 'Arkin|Adam P|AP|', 'Rao|Christopher V|CV|']
D000103:Acetyl-CoA Carboxylase D016960:Agrobacterium tumefaciens D001487:Basidiomycota D056804:Biofuels D051048:Diacylglycerol O-Acyltransferase D005227:Fatty Acids D016681:Genome, Fungal D005947:Glucose D050356:Lipid Metabolism D060847:Metabolic Engineering D014170:Transformation, Genetic D015854:Up-Regulation D014994:Xylose
Rhodosporidium toruloides lipid production Agrobacterium tumefaciens mediated transformation biodiesel genome sequencing