A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.
The ability to accurately identify and quantify immune cell populations within adipose tissue is important in understanding the role of immune cells in metabolic disease risk. Flow cytometry is the gold standard method for immune cell quantification. However, quantification of immune cells from adipose tissue presents a number of challenges because of the complexities of working with an oily substance and the rapid deterioration of immune cell viability before analysis can be performed. Here we present a highly reproducible flow cytometry protocol for the quantification of immune cells in human adipose tissue, which overcomes these issues.
['Delaney|Kerri Z|KZ|', 'Dam|Vi|V|', 'Murphy|Jessica|J|', 'Morais|José A|JA|', 'Denis|Ronald|R|', 'Atlas|Henri|H|', 'Pescarus|Radu|R|', 'Garneau|Pierre Y|PY|', 'Santosa|Sylvia|S|']
D000273:Adipose Tissue D000328:Adult D015703:Antigens, CD D015214:Antigens, Differentiation, Myelomonocytic D005260:Female D005434:Flow Cytometry D006801:Humans D017493:Leukocyte Common Antigens D008562:Membrane Glycoproteins D008875:Middle Aged D011971:Receptors, Immunologic D015203:Reproducibility of Results D013048:Specimen Handling
Flow cytometry Immune cells Humans Adipose tissue Inflammation Obesity