Characterization of specific CD4 and CD8 T-cell responses in QuantiFERON TB Gold-Plus TB1 and TB2 tubes.
The new generation of the IGRA QuantiFERON-TB Gold Plus (QFT-Plus) includes two antigen tubes, TB1 and TB2 which contain specific Mycobacterium tuberculosis peptides designed to stimulate both CD4 and CD8 T-cells. TB1 is designed to target cell mediated responses from CD4 T-cells, while TB2 contains newly designed peptides designed to also stimulate CD8 T-cells. We identified specific CD4 and CD8 T-cell clones that recognize different regions spanning the length of the CFP-10 protein in M. tuberculosisto directly test in QFT-Plus TB1 and TB2 tubes, followed by Interferon-gamma detection by the QFT-Plus ELISA. These clones showed specific responses to the different QFT-Plus tubes, the CD4 T-cell clone showed dose-dependent responses to both TB1 and TB2 tubes, while the CD8 T-cell clones showed specific and targeted responses to the QFT-Plus TB2 tube (>140-fold difference) versus the QFT-Plus TB1 tubes using the QFT-Plus ELISA. This testing provides direct evidence of the specificity of CD8 T-cell mediated response in QFT-Plus TB2 tubes.
['Allen|Nadia Pc|NP|', 'Swarbrick|Gwendolyn|G|', 'Cansler|Meghan|M|', 'Null|Megan|M|', 'Salim|Hiam|H|', 'Miyamasu|Misato|M|', 'Howard|Jenny|J|', 'Boyle|Jeffrey|J|', 'Lewinsohn|David|D|', 'Lewinsohn|Deborah|D|']
D001426:Bacterial Proteins D015496:CD4-Positive T-Lymphocytes D018414:CD8-Positive T-Lymphocytes D004797:Enzyme-Linked Immunosorbent Assay D004867:Equipment Design D054884:Host-Pathogen Interactions D006801:Humans D016056:Immunodominant Epitopes D007371:Interferon-gamma D059425:Interferon-gamma Release Tests D009169:Mycobacterium tuberculosis D011237:Predictive Value of Tests D015203:Reproducibility of Results D014376:Tuberculosis
CD8 T-cell responses QuantiFERON QFT-Plus CD4 T-cell responses Tuberculosis (TB)