Reactive Oxygen Species Produced by a Photodynamic Effect Induced Calcium Signal in Neurons and Astrocytes.
Photodynamic therapy (PDT) leads to production of reactive oxygen species (ROS) and cell destruction due to oxidative stress. We used photodynamic effect of photosensitizer radachlorin to unravel the effect of photo-induced oxidative stress on the calcium signal and lipid peroxidation in primary culture of cortical neurons and astrocytes using live cell imaging. We have found that irradiation in presence of 200 nM of radachlorin induces calcium signal in primary neurons and astrocytes. Photo-induced neuronal calcium signal depends on internal calcium stores as it was still observed in calcium-free medium and could be blocked by depletion of endoplasmic reticulum (ER) stores with inhibitor of sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) thapsigargin. Both inhibitors of phospholipase C activity U73122 and water-soluble analogue of vitamin E Trolox suppressed calcium response activated by PDT. We have also observed that the photodynamic effect of radachlorin induces lipid peroxidation in neurons and astrocytes. This data demonstrate that lipid peroxidation induced by PDT in neurons and astrocytes leads to activation of phospholipase C that results in production of inositol 1,4,5-trisphosphate (IP3).
['Neginskaya|Maria|M|', 'Berezhnaya|Elena|E|', 'Uzdensky|Anatoly B|AB|', 'Abramov|Andrey Y|AY|']
D000818:Animals D001253:Astrocytes D020013:Calcium Signaling D018920:Coculture Techniques D004338:Drug Combinations D007834:Lasers D009474:Neurons D017319:Photosensitizing Agents D011166:Porphyrins D051381:Rats D017207:Rats, Sprague-Dawley D017382:Reactive Oxygen Species
Photodynamic effect Calcium signaling Neurons Astrocytes Radachlorin