pH-Triggered Polypeptides Nanoparticles for Efficient BODIPY Imaging-Guided Near Infrared Photodynamic Therapy.
An efficient pH-responsive multifunctional polypeptide micelle for simultaneous imaging and in vitro photodynamic therapy (PDT) has been prepared. The goal here is to detect and treat cancer cells by near-infrared fluorescence (NIRF) imaging and PDT synchronously. A photosensitizer BODIPY-Br2 with efficient singlet oxygen generation was synthesized at first which owns both seductive abilities in fluorescence emission and reactive oxygen species (ROS) generation under light irradiation. Then, amphiphilic copolymer micelles pH-triggered disassembly were synthesized from N-carboxyanhydride (NCA) monomer via a ring-opening polymerization and click reaction for the loading of BODIPY-Br2 by hydrophobic interaction, and the driving force is the protonation of the diisopropylethylamine groups conjugated to the polypeptide side chains. In vitro tests performed on HepG2 cancer cells confirm that the cell suppression rate was improved by more than 40% in the presence of light in the presence of an extremely low energy density (12 J/cm(2)) with very low concentration of 5.4 μM photosensitizer. At the same time, the internalization of the nanoparticles by cells can also be traced by NIRF imaging, indicating that the NIR nanoparticles presented imaging guided photodynamic therapy properties. It provides the potential of using polypeptide as a biodegradable carrier for NIR image-guided photodynamic therapy.
['Liu|Le|L|', 'Fu|Liyi|L|', 'Jing|Titao|T|', 'Ruan|Zheng|Z|', 'Yan|Lifeng|L|']
D000812:Anhydrides D063566:Biodegradable Plastics D004337:Drug Carriers D005453:Fluorescence D056945:Hep G2 Cells D006801:Humans D006863:Hydrogen-Ion Concentration D007259:Infrared Rays D053758:Nanoparticles D009369:Neoplasms D010455:Peptides D010778:Photochemotherapy D017319:Photosensitizing Agents D011108:Polymers D017382:Reactive Oxygen Species D026082:Singlet Oxygen
polypeptide pH-responsive photodynamic therapy (PDT) near-infrared fluorescence (NIRF) imaging singlet oxygen N-carboxyanhydride (NCA)