Plasma Proteome Profiles of Stable CAD Patients Stratified According to Total Apo C-III Levels.
PURPOSE
The present research reports the study the of plasma proteome profile of stable coronary artery disease (CAD) patients characterized by different levels of total Apolipoprotein-CIII (Apo C-III), a prognostic marker for cardiovascular risk.


EXPERIMENTAL DESIGN
Two subgroups of CAD patients (n = 52) with divergent concentrations of total circulating Apo C-III (≤ and ≥10 mg dL-1 ) are examined using a shotgun proteomic approach. Validation experiments are also performed with immunochemistry methods including both the patients affected by CAD (n = 119) and the subjects without CAD (CAD-free; n = 58). Results are analyzed by bioinformatics tools and multivariate statistics.


RESULTS
A total of 188 proteins are quantified among the patients. The fold change analysis and the partial least square discriminant analysis show a clear separation of the two groups. Lipoproteins (Apo C-II and Apo E), retinol-binding protein 4, and vitronectin are upregulated in patients with high Apo C-III, while alpha-1 antitrypsin is downregulated.


CONCLUSIONS AND CLINICAL RELEVANCE
In this pilot study, the differential expression of plasma proteins related to different concentrations of Apo C-III is defined, suggesting possible new players involved in the Apo C-III-associated process of arterial damage. Data are available via ProteomeXchange with identifier PXD005973.
['Manfredi|Marcello|M|', 'Chiariello|Carmela|C|', 'Conte|Eleonora|E|', 'Castagna|Annalisa|A|', 'Robotti|Elisa|E|', 'Gosetti|Fabio|F|', 'Patrone|Mauro|M|', 'Martinelli|Nicola|N|', 'Bassi|Antonella|A|', 'Cecconi|Daniela|D|', 'Marengo|Emilio|E|', 'Olivieri|Oliviero|O|']
D053305:Apolipoprotein C-III D003324:Coronary Artery Disease D005260:Female D006801:Humans D008297:Male D008875:Middle Aged D015999:Multivariate Analysis D010865:Pilot Projects D011379:Prognosis D040901:Proteomics
plasma proteomics Apo C-III coronary artery disease apolipoproteins cardiovascular disease