Expression and localization of fibroblast growth factor (FGF)23 and Klotho in the spleen: its physiological and functional implications.
The FGF23-Klotho signaling axis is known to exert anti-aging effects via calcium-phosphorus metabolism. In mice deficient in FGF23-Klotho signaling, however, the number of splenocytes is reduced. FGF23 is expressed in both bone and spleen, with regulation of its production differing in these organs. As FGF23-Klotho signaling may play an immunological role in the spleen, splenocytes in male C57BL/6J mice were assayed for expression of Klotho or FGF23 by flow cytometry and immunohistochemistry. Cells that expressed Klotho included CD45R/B220+ CD21/CD35+ CD1d+ CD43- marginal zone B cells. These cells also expressed FGF receptor 1, indicating that Klotho-positive B cells could respond to FGF23. Plasmacytoid dendritic cells (pDCs) with CD11c+ CD45R/B220+ CD11b- CD8α- were found to produce FGF23. Klotho-positive cells and FGF23-producing cells were present in close proximity to each other, suggesting that FGF23 produced by pDCs may act within a limited area. These findings indicate that FGF23-Klotho signaling could play a biological or immunological role in the spleen.
['Nakashima|Yuri|Y|', 'Mima|Toru|T|', 'Yashiro|Mitsuru|M|', 'Sonou|Tomohiro|T|', 'Ohya|Masaki|M|', 'Masumoto|Asuka|A|', 'Yamanaka|Shintaro|S|', 'Koreeda|Daisuke|D|', 'Tatsuta|Koichi|K|', 'Hanba|Yoshiyuki|Y|', 'Moribata|Mari|M|', 'Negi|Shigeo|S|', 'Shigematsu|Takashi|T|']
D000818:Animals D001402:B-Lymphocytes D003713:Dendritic Cells D000089703:Fibroblast Growth Factor-23 D005346:Fibroblast Growth Factors D005966:Glucuronidase D000090265:Klotho Proteins D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D017468:Receptors, Fibroblast Growth Factor D013154:Spleen
Klotho splenocytes FGF23 B cells dendritic cells