Global real-time quantitative reverse transcription-polymerase chain reaction detecting proto-oncogenes associated with 14q32 chromosomal translocation as a valuable marker for predicting survival in multiple myeloma.
CCND1, FGFR3 and c-MAF mRNA expression of tumor samples from 123 multiple myeloma patients were analyzed by global RQ/RT-PCR. CCND1, FGFR3 and c-MAF were positive in 44 (36%), 28 (23%) and 16 (13%) of patients, respectively. In 7 patients, both FGFR3 and c-MAF were positive. The expression of c-MAF was independent unfavorable prognostic factors for overall survival (OS). Autologous stem cell transplantation improved progression-free survival of CCND1-positive patients. Bortezomib, thalidomide or lenalidomide extended OS of FGFR3 and/or c-MAF-positive patients. Thus, CCND1, FGFR3 and c-MAF mRNA expression can predict survival and is useful for planning stratified treatment strategies for myeloma patients.
['Inagaki|Atsushi|A|', 'Tajima|Emi|E|', 'Uranishi|Miyuki|M|', 'Totani|Haruhito|H|', 'Asao|Yu|Y|', 'Ogura|Hiroka|H|', 'Masaki|Ayako|A|', 'Yoshida|Tatsuya|T|', 'Mori|Fumiko|F|', 'Ito|Asahi|A|', 'Yano|Hiroki|H|', 'Ri|Masaki|M|', 'Kayukawa|Satoshi|S|', 'Kataoka|Takae|T|', 'Kusumoto|Shigeru|S|', 'Ishida|Takashi|T|', 'Hayami|Yoshihito|Y|', 'Hanamura|Ichiro|I|', 'Komatsu|Hirokazu|H|', 'Inagaki|Hiroshi|H|', 'Matsuda|Yasufumi|Y|', 'Ueda|Ryuzo|R|', 'Iida|Shinsuke|S|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D014408:Biomarkers, Tumor D016022:Case-Control Studies D002883:Chromosomes, Human, Pair 14 D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008297:Male D008875:Middle Aged D009101:Multiple Myeloma D011379:Prognosis D011519:Proto-Oncogenes D060888:Real-Time Polymerase Chain Reaction D016019:Survival Analysis D014178:Translocation, Genetic
Survival Multiple myeloma CCND1 FGFR3 C-MAF FISH PCR