pH- and redox-responsive poly(ethylene glycol) and cholesterol-conjugated poly(amido amine)s based micelles for controlled drug delivery.
An optimized condition is identified to prepare linear poly(amido amine)s via Michael Addition polymerization of trifunctional amine, 4-(aminomethyl)piperidine (AMPD), with an equimolar diacrylamide, N,N-cystaminebis(acrylamide) (BAC). Poly(ethylene glycol) (PEG) and cholesterol (CE) are conjugated to linear poly(BAC-AMPD) through the reactions with the secondary amino groups in the backbone, respectively, to form poly(BAC-AMPD)-g-PEG-g-CE. The chemical structures of poly(BAC-AMPD) and poly(BAC-AMPD)-g-PEG-g-CE are characterized using NMR and gel permeation chromatography (GPC). Transmission electron microscopy (TEM), dynamic light scattering (DLS) and (1)H NMR results show that micelles with PEG shells and hydrophobic cores composed of poly(BAC-AMPD) and CE are formed via self-assembly of poly(BAC-AMPD)-g-PEG-g-CE in aqueous solution, and the micelles of poly(BAC-AMPD)-g-PEG-g-CE can be degraded by the presence of L-dithiothreitol and show a limited cytotoxicity in vitro. The anti-cancer drug, doxorubicin (DOX), can be loaded into the micelles. The DOX loaded micelles of poly(BAC-AMPD)-g-PEG-g-CE show pH- and redox-responsive drug release and redox-induced formation of aggregates, and it is shown that the DOX loaded micelles can deliver DOX into cells and show a higher efficacy in killing cancer cells than free drug.
['Cheng|Weiren|W|', 'Kumar|Jatin N|JN|', 'Zhang|Yong|Y|', 'Liu|Ye|Y|']
D000178:Acrylamides D000903:Antibiotics, Antineoplastic D045744:Cell Line, Tumor D002470:Cell Survival D002784:Cholesterol D003692:Delayed-Action Preparations D004317:Doxorubicin D006801:Humans D006863:Hydrogen-Ion Concentration D057927:Hydrophobic and Hydrophilic Interactions D008823:Micelles D010084:Oxidation-Reduction D010316:Particle Size D011073:Polyamines D011092:Polyethylene Glycols
poly(amido amine)s self-assembly drug delivery systems redox polymers stimuli-responsive polymers