Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system.
We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system for hyperspectral imaging in thick tissue. We use a strategy where the Raman lines are excited sequentially, circumventing the need for a spectrometer. This fibre laser system, consisting of a pump laser synchronized with a rapidly tunable programmable laser (PL), can access Raman lines over a significant fraction of the high wavenumber region (2700-2950 cm(-1)) at rates of up to 10,000 spectral points per second. To demonstrate its capabilities, we have acquired WS-CARS spectra of several samples as well as images and hyperspectral images (HSI) of thick tissue both in forward and epi-detection. This instrument should be especially useful in providing local biochemical information with surrounding context supplied by imaging.
['Bégin|Steve|S|', 'Burgoyne|Bryan|B|', 'Mercier|Vincent|V|', 'Villeneuve|Alain|A|', 'Vallée|Réal|R|', 'Côté|Daniel|D|']
(170.3880) Medical and biological imaging (180.4315) Nonlinear microscopy (300.6230) Spectroscopy, coherent anti-Stokes Raman scattering