Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1.
Protein biosynthesis is energetically costly, is tightly regulated and is coupled to stress conditions including glucose deprivation. RNA polymerase III (RNAP III)-driven transcription of tDNA genes for production of tRNAs is a key element in efficient protein biosynthesis. Here we present an analysis of the effects of altered RNAP III activity on the Saccharomyces cerevisiae proteome and metabolism under glucose-rich conditions. We show for the first time that RNAP III is tightly coupled to the glycolytic system at the molecular systems level. Decreased RNAP III activity or the absence of the RNAP III negative regulator, Maf1 elicit broad changes in the abundance profiles of enzymes engaged in fundamental metabolism in S. cerevisiae In a mutant compromised in RNAP III activity, there is a repartitioning towards amino acids synthesis de novo at the expense of glycolytic throughput. Conversely, cells lacking Maf1 protein have greater potential for glycolytic flux.
['Szatkowska|Roza|R|0000-0003-4494-5236', 'Garcia-Albornoz|Manuel|M|0000-0002-2300-2047', 'Roszkowska|Katarzyna|K|', 'Holman|Stephen W|SW|', 'Furmanek|Emil|E|', 'Hubbard|Simon J|SJ|', 'Beynon|Robert J|RJ|0000-0003-0857-495X', 'Adamczyk|Malgorzata|M|0000-0003-3979-0364']
D020869:Gene Expression Profiling D005800:Genes, Fungal D005947:Glucose D051246:Glucose Transport Proteins, Facilitative D006019:Glycolysis D053858:Metabolic Networks and Pathways D008954:Models, Biological D010427:Pentose Phosphate Pathway D017354:Point Mutation D020543:Proteome D012320:RNA Polymerase III D012441:Saccharomyces cerevisiae D029701:Saccharomyces cerevisiae Proteins D015398:Signal Transduction D014157:Transcription Factors
RNA polymerase III maf1 amino acid metabolism comparative proteomics glycolysis