Hyaluronan and fibrin biomaterial as scaffolds for neuronal differentiation of adult stem cells derived from adipose tissue and skin.
Recently, we have described a simple protocol to obtain an enriched culture of adult stem cells organized in neurospheres from two post-natal tissues: skin and adipose tissue. Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue engineering application: hyaluronan based membranes and fibrin-glue meshes. Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes. Neurospheres were then induced to acquire a glial and neuronal-like phenotype. Gene expression, morphological feature and chromosomal imbalance (kariotype) were analyzed and compared. Adipose and skin derived neurospheres are able to grow well and to differentiate into glial/neuron cells without any chromosomal imbalance in both scaffolds. Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we have demonstrated that neurospheres isolated from skin and adipose tissues are able to differentiate in glial/neuron-like cells, without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane and fibrin-glue meshes.
['Gardin|Chiara|C|', 'Vindigni|Vincenzo|V|', 'Bressan|Eriberto|E|', 'Ferroni|Letizia|L|', 'Nalesso|Elisa|E|', 'Puppa|Alessandro Della|AD|', "D'Avella|Domenico|D|", 'Lops|Diego|D|', 'Pinton|Paolo|P|', 'Zavan|Barbara|B|']
D000273:Adipose Tissue D000328:Adult D053687:Adult Stem Cells D001672:Biocompatible Materials D018929:Cell Culture Techniques D002454:Cell Differentiation D043171:Chromosomal Instability D015718:Fibrin Tissue Adhesive D006801:Humans D006820:Hyaluronic Acid D007621:Karyotyping D008875:Middle Aged D009419:Nerve Tissue Proteins D009474:Neurons D012867:Skin D023822:Tissue Engineering D054457:Tissue Scaffolds
stem cells adipose tissue skin Schwann cell adipose derived stem cells karyotypes