Simultaneous ultrasound-assisted water extraction and β-cyclodextrin encapsulation of polyphenols from Mangifera indica stem bark in counteracting TNFα-induced endothelial dysfunction.
This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of β-cyclodextrin (β-CD) at room temperature and testing their biological activity on TNFα-induced endothelial dysfunction. Both extracts counteracted TNFα effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels. β-CD extract showed higher efficacy in improving endothelial function. These effects were abolished after pre-treatment with the oestrogen receptor inhibitor ICI1182,780. Moreover, the β-CD extract induced Akt activation and completely abolished the TNFα-induced p38MAPK phosphorylation. UAE and β-CD encapsulation provide an efficient extraction protocol that increases polyphenol bioavailability. Polyphenols from MI play a protective role on endothelial cells and may be further considered as oestrogen-like molecules with vascular protective properties.
['Mura|Marzia|M|', 'Palmieri|Daniela|D|', 'Garella|Davide|D|', 'Di Stilo|Antonella|A|', 'Perego|Patrizia|P|', 'Cravotto|Giancarlo|G|', 'Palombo|Domenico|D|']
D002460:Cell Line D051546:Cyclooxygenase 2 D042783:Endothelial Cells D006801:Humans D018799:Intercellular Adhesion Molecule-1 D015850:Interleukin-6 D016209:Interleukin-8 D031022:Mangifera D020778:Matrix Metalloproteinase 2 D020780:Matrix Metalloproteinase 9 D052250:Nitric Oxide Synthase Type III D010766:Phosphorylation D024301:Plant Bark D010936:Plant Extracts D059808:Polyphenols D015398:Signal Transduction D014409:Tumor Necrosis Factor-alpha D014465:Ultrasonics D047392:beta-Cyclodextrins D048051:p38 Mitogen-Activated Protein Kinases
β-cyclodextrin Mangifera indica endothelial dysfunction ultrasound-assisted extraction TNFα oestrogen receptor