Paenibacillus seodonensis sp. nov., isolated from a plant of the genus Campanula.
Strain DCT-19T, representing a Gram-stain-positive, rodshaped, aerobic bacterium, was isolated from a native plant belonging to the genus Campanula on Dokdo, the Republic of Korea. Comparative analysis of the 16S rRNA gene sequence showed that this strain was closely related to Paenibacillus amylolyticus NRRL NRS-290T (98.6%, 16S rRNA gene sequence similarity), Paenibacillus tundrae A10bT (98.1%), and Paenibacillus xylanexedens NRRL B-51090T (97.6%). DNADNA hybridization indicated that this strain had relatively low levels of DNA-DNA relatedness with P. amylolyticus NRRL NRS-290T (30.0%), P. xylanexedens NRRL B-51090T (29.0%), and P. tundrae A10bT (24.5%). Additionally, the genomic DNA G + C content of DCT-19T was 44.8%. The isolated strain grew at pH 6.0-8.0 (optimum, pH 7.0), 0-4% (w/v) NaCl (optimum, 0%), and a temperature of 15-45°C (optimum 25-30°C). The sole respiratory quinone in the strain was menaquinone-7, and the predominant fatty acids were C15:0 anteiso, C16:0 iso, and C16:0. In addition, the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. Based on its phenotypic properties, genotypic distinctiveness, and chemotaxonomic features, strain DCT-19T is proposed as a novel species in the genus Paenibacillus, for which the name Paenibacillus seodonensis sp. nov. is proposed (=KCTC 43009T =LMG 30888T). The type strain of Paenibacillus seodonensis is DCT-19T.
['Kang|Myung-Suk|MS|', 'Lee|Ki-Eun|KE|', 'Lee|Eun-Young|EY|', 'Park|Soo-Je|SJ|']
D015373:Bacterial Typing Techniques D001482:Base Composition D029422:Campanulaceae D004269:DNA, Bacterial D005227:Fatty Acids D005838:Genotype D009693:Nucleic Acid Hybridization D056507:Paenibacillus D010743:Phospholipids D010802:Phylogeny D012336:RNA, Ribosomal, 16S D056910:Republic of Korea D017422:Sequence Analysis, DNA D012988:Soil Microbiology D013045:Species Specificity D013696:Temperature
Paenibacillus seodonensis sp. nov. Campanula Dokdo novel species polyphasic