A novel textile dye degrading extracellular laccase from symbiotic bacterium of Bacillus sp. CF96 isolated from gut termite (Anacanthotermes).
Oxidation of phenolic compounds is an urgent need in textile industry, biological refinements, pulp and paper production. In present study, a laccase was purified from symbiotic bacterium of Bacillus sp. CF96 existing in termite digestive system. The extracellular laccase was purified via amnion sulfate precipitation, membrane dialysis, and ion exchange chromatography. The results showed that the Bacillus CF96 laccase possesses a molecular mass of 63 kDa, an optimal pH and temperature of 8.0 and 60 °C. Results showed that Zn2+, Mn2+ and Fe2+ were considered as the activator ions, while SDS was the main inhibitor. Using syringaldazine (SGZ) as substrate, the half-life of laccase at optimal temperature was 148 min; Km and Vmax were 0.737 μM and 100.5 U/mg. In addition, the enzyme showed a high effect on indigo dye with 90% bleaching capacity compared to control. In conclusion, the laccase has potential applications in industries under the provided optimal conditions.
['Javadzadeh|Seyedeh-Golnaz|SG|', 'Asoodeh|Ahmad|A|']
D000548:Amaranth Dye D000818:Animals D001407:Bacillus D001426:Bacterial Proteins D001673:Biodegradation, Environmental D004396:Coloring Agents D003224:Congo Red D004795:Enzyme Stability D000069196:Gastrointestinal Microbiome D006863:Hydrogen-Ion Concentration D006868:Hydrolysis D007203:Indigo Carmine D020049:Isoptera D042845:Laccase D008970:Molecular Weight D010084:Oxidation-Reduction D013379:Substrate Specificity D013559:Symbiosis D013696:Temperature D013783:Textile Industry D014048:Tolonium Chloride
Laccase Bacillus sp. CF96 Termite Bleaching Purification