Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense.
This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.
['Kim|Song Yee|SY|', 'Kang|Young Ae|YA|', 'Bae|Il Kwon|IK|', 'Yim|Jae-Joon|JJ|', 'Park|Moo Suk|MS|', 'Kim|Young Sam|YS|', 'Kim|Se Kyu|SK|', 'Chang|Joon|J|', 'Jeong|Seok Hoon|SH|']
D001426:Bacterial Proteins D004269:DNA, Bacterial D005260:Female D006801:Humans D007223:Infant D007231:Infant, Newborn D008297:Male D058885:Multilocus Sequence Typing D009161:Mycobacterium D009164:Mycobacterium Infections D012189:Retrospective Studies
Multilocus sequence typing Mycobacterium abscessus Mycobacterium massiliense Multilocus sequence analysis rpoB