Modulation of Cholesterol-Related Gene Expression by Dietary Fiber Fractions from Edible Mushrooms.
Mushrooms are a source of dietary fiber (DF) with a cholesterol-lowering effect. However, their underlying mechanisms are poorly understood. The effect of DF-enriched fractions from three mushrooms species on cholesterol-related expression was studied in vitro. The Pleurotus ostreatus DF fraction (PDF) was used in mice models to assess its potential palliative or preventive effect against hypercholesterolemia. PDF induced a transcriptional response in Caco-2 cells, suggesting a possible cholesterol-lowering effect. In the palliative setting, PDF reduced hepatic triglyceride likely because Dgat1 was downregulated. However, cholesterol-related biochemical data showed no changes and no relation with the observed transcriptional modulation. In the preventive setting, PDF modulated cholesterol-related genes expression in a manner similar to that of simvastatin and ezetimibe in the liver, although no changes in plasma and liver biochemical data were induced. Therefore, PDF may be useful reducing hepatic triglyceride accumulation. Because it induced a molecular response similar to hypocholesterolemic drugs in liver, further dose-dependent studies should be carried out.
['Caz|Víctor|V|', 'Gil-Ramírez|Alicia|A|', 'Largo|Carlota|C|', 'Tabernero|María|M|', 'Santamaría|Mónica|M|', 'Martín-Hernández|Roberto|R|', 'Marín|Francisco R|FR|', 'Reglero|Guillermo|G|', 'Soler-Rivas|Cristina|C|']
D000364:Agaricus D000818:Animals D001835:Body Weight D018938:Caco-2 Cells D002784:Cholesterol D004043:Dietary Fiber D019587:Dietary Supplements D005243:Feces D005786:Gene Expression Regulation D006801:Humans D006937:Hypercholesterolemia D008099:Liver D008297:Male D008810:Mice, Inbred C57BL D020076:Pleurotus D020942:Shiitake Mushrooms
dietary fibers Pleurotus ostreatus Agaricus bisporus Lentinus edodes cholesterol low-density array (LDA) oyster mushroom polysaccharides shiitake mushroom white button mushroom β-glucans