Cytoprotective Activity of p-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells.
The influence of p-terphenyl polyketides 1-3 from Aspergillus candidus KMM 4676 and cerebroside flavuside B (4) from Penicillium islandicum (=Talaromyces islandicus) against the effect of neurotoxins, rotenone and paraquat, on Neuro-2a cell viability by MTT and LDH release assays and intracellular ROS level, as well as DPPH radical scavenging activity, was investigated. Pre-incubation with compounds significantly diminished the ROS level in rotenone- and paraquat-treated cells. It was shown that the investigated polyketides 1-3 significantly increased the viability of rotenone- and paraquat-treated cells in two of the used assays but they affected only the viability of paraquat-treated cells in the LDH release assay. Flavuside B statistically increased the viability of paraquat-treated cells in both MTT and LDH release assays, however, it increased the viability of rotenone-treated cells in the LDH release assay. Structure-activity relationships for p-terphenyl derivatives, as well as possible mechanisms of cytoprotective action of all studied compounds, were discussed.
['Yurchenko|Ekaterina A|EA|0000-0001-7737-0980', 'Menchinskaya|Ekaterina S|ES|', 'Pislyagin|Evgeny A|EA|0000-0002-3558-0821', 'Chingizova|Ekaterina A|EA|', 'Girich|Elena V|EV|', 'Yurchenko|Anton N|AN|0000-0001-6816-3605', 'Aminin|Dmitry L|DL|', 'Mikhailov|Valery V|VV|']
D000818:Animals D001230:Aspergillus D045744:Cell Line, Tumor D002470:Cell Survival D019610:Cytoprotection D006028:Glycosphingolipids D006540:Herbicides D007306:Insecticides D051379:Mice D009447:Neuroblastoma D018696:Neuroprotective Agents D009498:Neurotoxins D010269:Paraquat D061065:Polyketides D017382:Reactive Oxygen Species D012402:Rotenone
cytoprotection rotenone paraquat marine fungi reactive oxygen species secondary metabolites