Chemical-Labeling-Assisted Detection of Nucleobase Modifications by Quantum-Tunneling-Based Single-Molecule Sensing.
Quantum-tunneling-based DNA sensing is a single-molecule technique that promises direct mapping of nucleobase modifications. However, its applicability is seriously limited because of the small difference in conductivity between modified and unmodified nucleobases. Herein, a chemical labeling strategy is presented that facilitates the detection of modified nucleotides by quantum tunneling. We used 5-Formyl-2'-deoxyuridine as a model compound and demonstrated that chemical labeling dramatically alters its molecular conductance compared with that of canonical nucleotides; thus, facilitating statistical discrimination, which is impeded in the unlabeled state. This work introduces a chemical strategy that overcomes the intrinsic difficulty in quantum-tunneling-based modification analysis-the similarity of the molecular conductance of the nucleobases of interest.
['Furuhata|Takafumi|T|', 'Ohshiro|Takahito|T|', 'Izuhara|Yuichi|Y|', 'Suzuki|Tomoaki|T|', 'Ueki|Ryosuke|R|0000-0002-5433-5354', 'Taniguchi|Masateru|M|', 'Sando|Shinsuke|S|0000-0003-0275-7237']
D004247:DNA D003857:Deoxyuridine D004553:Electric Conductivity D015394:Molecular Structure D011789:Quantum Theory
nucleobases single-molecule sensing chemical labeling quantum tunneling biosensors