One-step multiplex reverse transcription-polymerase chain reaction for the simultaneous detection of three rice viruses.
Rice stripe virus (RSV), Rice black-streaked dwarf virus (RBSDV), and Rice dwarf virus (RDV) are major rice-infecting viruses in Korea that can cause serious crop losses. A one-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for the simultaneous detection of these rice viruses. Three sets of specific primers targeted to the capsid protein coding genes of RSV, RBSDV, and RDV were used to amplify fragments that were 703 bp, 485 bp, and 252 bp, respectively. The one-step mRT-PCR assay proved to be a sensitive and rapid method for detecting the three rice viruses. This method could be used to facilitate better control of rice viruses.
['Cho|Sang-Yun|SY|', 'Jeong|Rae-Dong|RD|', 'Yoon|Young-Nam|YN|', 'Lee|Su-Heon|SH|', 'Shin|Dong Bum|DB|', 'Kang|Hang-Won|HW|', 'Lee|Bong Choon|BC|']
D036022:Capsid Proteins D017931:DNA Primers D007723:Korea D060885:Multiplex Polymerase Chain Reaction D012275:Oryza D010935:Plant Diseases D012087:Reoviridae D020133:Reverse Transcriptase Polymerase Chain Reaction D012680:Sensitivity and Specificity D029364:Tenuivirus D013997:Time Factors D014773:Virology
Simultaneous detection Rice stripe virus Rice black-streaked dwarf virus Rice dwarf virus One-step multiplex RT-PCR