Cytogenetic tests reveal no toxicity in lymphocytes of rabbit (Oryctolagus cuniculus, 2n=44) feed in presence of verbascoside and/or lycopene.
Phenylpropanoid glycosides (PPG), like other phenolic compounds, are a powerful antioxidants and the Verbascoside (VB) is one of the most active of them. A previous study, by using in vitro exposure of blood human lymphocytes to Verbascoside, reported a significant increasings of chromosome fragility compared to control. In the present study, four homogeneous groups of rabbits were used to test in vivo the VB and/or Lycopene (LP) by feeding the animals without VB and LP (control), in presence of VB or/and LP for 80 days. Lymphocyte cell cultures were performed in three different times: 0, 40 and 80 days of the experiment and the cytogenetic tests that we used [CA-test (Chromosome Abnormalities in terms of chromosome and chromatid breaks) and Sister Chromatid Exchange (SCE-test)] have revealed no mutagenic effects on chromosomes. Indeed, mean values/cell of CA and SCE decreased during the experiment with some difference among and within groups, with significant decreasing value only for some group. The study shows clear evidence that diets rich in Verbascoside (and/or Lycopene) do not originate any mutagenic activity, resulting no cytotoxic for the animals and, suggesting a possible their use in both animal and human diets.
['Perucatti|Angela|A|', 'Genualdo|Viviana|V|', 'Pauciullo|Alfredo|A|', 'Iorio|Ciro|C|', 'Incarnato|Domenico|D|', 'Rossetti|Cristina|C|', 'Vizzarri|Francesco|F|', 'Palazzo|Marisa|M|', 'Casamassima|Donato|D|', 'Iannuzzi|Leopoldo|L|', 'Iannuzzi|Alessandra|A|']
D000821:Animal Feed D000818:Animals D002338:Carotenoids D002478:Cells, Cultured D002869:Chromosome Aberrations D003582:Cytogenetics D005960:Glucosides D000077276:Lycopene D008214:Lymphocytes D008297:Male D010636:Phenols D011817:Rabbits D012854:Sister Chromatid Exchange
Verbascoside Lycopene Chromosome stability In vivo exposure Mutagenic test