Extraction, purification, and antioxidant activities of polysaccharides from Tricholoma mongolicum Imai.
Ultrasonic-microwave synergistic extraction (UMSE), purification, and characterization of Tricholoma mongolicum Imai polysaccharides (TMIPs) from fruit bodies were investigated in this study. Response surface methodology (RSM) was employed to optimize the UMSE conditions. The results indicated that the optimal extraction conditions were as follows: extraction time of 24.65 min, microwave power of 109.98 W, and water to raw material ratio of 21.62 ml/g. Under optimized conditions, the yield and purity of TMIPs were 35.41 ± 0.62% and 73.92 ± 0.83%, respectively. Crude TMIPs were purified by DEAE-Cellulose 52 chromatography and Sephadex G-100 chromatography to afford four fractions, namely, TMIP-1, TMIP-2, TMIP-3, and TMIP-4. Preliminary TMIP characterization was conducted by Fourier transform infrared spectroscopy. TMIP antioxidant activities were investigated by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radicals, ferric reducing activity power, and reducing power assay. The results indicated that TMIPs have good antioxidant activity.
['You|Qinghong|Q|', 'Yin|Xiulian|X|', 'Zhang|Shengnan|S|', 'Jiang|Zhonghai|Z|']
D000975:Antioxidants D001713:Biphenyl Compounds D002850:Chromatography, Gel D002852:Chromatography, Ion Exchange D005163:Factor Analysis, Statistical D048690:Fruiting Bodies, Fungal D062610:Fungal Polysaccharides D017665:Hydroxyl Radical D008872:Microwaves D010851:Picrates D052617:Solid Phase Microextraction D055439:Tricholoma
Antioxidant activities Polysaccharides Tricholoma mongolicum Imai Ultrasonic-microwave synergistic extraction Response surface methodology