Cell Proliferation Stimulation Ability and Osteogenic Activity of Low Molecular Weight Peptides Derived from Bovine Gelatin Hydrolysates.
It has been recognized that collagen peptides of MW 878 Da (CP1) promote osteoblast proliferation and mineralization. The objective of this study is to identify the peptides responsible for proliferation of osteoblast growth, enhancement of ALP (alkaline phosphatase) activity in osteoblasts and promotion of osteoblast mineralization. To this end, the CP1 were fractioned by a series of chromatography procedures, and 51 peptides from the fraction possessing the most powerful cell proliferation ability were identified by LC-MS-MS. The peptides, GPAGPSGPAGK and GPPGSPGPR, were validated on a simultaneous basis as possessing enhanced bioactivity-inducing properties. In particular, the ALP activity of the cells treated with these two peptides was almost twice that of the control cells. Hydrogen bonds were formed, and the hydrophobic interactions with the EGFR (epidermal growth factor receptor) might be responsible for the osteoblast proliferation activity. On this basis, the two peptides might be potential lead compounds against osteoporosis and osteoarthritis.
['Wang|Jianing|J|http://orcid.org/0000-0003-3802-4270', 'Zhang|Bing|B|', 'Lu|Weipeng|W|', 'Liu|Junli|J|', 'Zhang|Weijie|W|', 'Wang|Yihu|Y|', 'Ma|Ming|M|http://orcid.org/0000-0001-6957-5644', 'Cao|Xiaofeng|X|', 'Guo|Yanchuan|Y|']
D000818:Animals D002417:Cattle D002460:Cell Line D049109:Cell Proliferation D005780:Gelatin D013058:Mass Spectrometry D051379:Mice D008970:Molecular Weight D010006:Osteoblasts D010012:Osteogenesis D010455:Peptides D011492:Protein Hydrolysates
cell proliferation stimulation osteogenic activity bovine gelatin hydrolysates bioactive peptides tandem mass spectrometry