Effects of CCK-8 and GLP-1 on fatty acid sensing and food intake regulation in trout.
We hypothesize that cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) are involved in the modulation of metabolic regulation of food intake by fatty acids in fish. Therefore, we assessed in rainbow trout (Oncorhynchus mykiss) the effects of intracerebroventricular treatment with 1 ng/g of CCK-8 and with 2 ng/g of GLP-1 on food intake, expression of neuropeptides involved in food intake control and the activity of fatty acid-sensing systems in hypothalamus and hindbrain. Food intake decreased up to 24 h post-treatment to 49.8-72.3% and 3.1-17.8% for CCK-8 and GLP-1, respectively. These anorectic responses are associated with changes in fatty acid metabolism and an activation of fatty acid-sensing mechanisms in the hypothalamus and hindbrain. These changes occurred in parallel with those in the expression of anorexigenic and orexigenic peptides. Moreover, we observed that the activation of fatty acid sensing and the enhanced anorectic potential elicited by CCK-8 and GLP-1 treatments occurred in parallel with the activation of mTOR and FoxO1 and the inhibition of AMPKα, BSX and CREB. The results are discussed in the context of metabolic regulation of food intake in fish.
['Velasco|Cristina|C|', 'Comesaña|Sara|S|', 'Conde-Sieira|Marta|M|', 'Míguez|Jesús M|JM|', 'Soengas|José L|JL|']
D000818:Animals D002766:Cholecystokinin D004435:Eating D005227:Fatty Acids D052216:Glucagon-Like Peptide 1 D007031:Hypothalamus D010446:Peptide Fragments D012249:Rhombencephalon D014337:Trout
CCK-8 GLP-1 fatty acid sensing fish hypothalamus hindbrain