N160 of Aiolos Determines its DNA-Binding Activity.
Aiolos is a transcription factor of the Ikaros family. It is expressed in lymphocytes and plays a vital role in the development of lymphatic system. Recently, Aiolos has been found to be ectopically expressed in lung cancer cells and promotes cancer metastasis through repression of p66Shc expression. Blocking DNA-binding activity of Aiolos may benefit cancer patients by preventing further metastasis. However, it is not clear which sequence is essential for its DNA binding. In this study, we found that N160 is a key amino acid to determine the DNA-binding activity of Aiolos. Mutation of N160A resulted in loss of peripheral heterochromatin localization and dissociation with its target gene. Accordingly, Aiolos with N160A could not change the expression of its target genes. Thus, we identified the amino acid (N160) that is essential for Aiolos binding to DNA. Anat Rec, 302:2014-2019, 2019. © 2019 American Association for Anatomy.
['Wang|Hao|H|0000-0002-2438-6732', 'Xu|Zhao|Z|', 'Du|Wei|W|', 'Lin|Zhenzhen|Z|', 'Liu|Zhe|Z|']
D000072283:A549 Cells D000596:Amino Acids D004247:DNA D004268:DNA-Binding Proteins D006801:Humans D051740:Ikaros Transcription Factor D009154:Mutation D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1
N160 Aiolos DNA binding transcriptional regulation