Protocol of Phytophthora capsici Transformation Using the CRISPR-Cas9 System.
Phytophthora capsici is an important plant pathogen, which causes significant economic losses on multiple vegetable crops worldwide. It is an ideal model pathogen to study the role of important genes, plant-pathogen interactions, and fungicide resistance mechanisms etc. due to its wide range of hosts and genetic diversity. A more efficient gene editing tool is required to do these studies. Here, we describe a detailed experimental procedure using the CRISPR-Cas9 system to edit genes of interest in P. capsici, which has been proven to be an accurate and efficient gene editing method in P. capsici.
['Wang|Zhiwen|Z|', 'Tyler|Brett M|BM|', 'Liu|Xili|X|']
D064113:CRISPR-Cas Systems D000072669:Gene Editing D023061:Gene Order D005822:Genetic Vectors D059765:Homologous Recombination D009154:Mutation D010838:Phytophthora D011523:Protoplasts D017394:RNA, Guide D014162:Transfection D014170:Transformation, Genetic
Phytophthora capsici CRISPR-Cas9 Gene edit Protoplast transformation