Generation of Avian Induced Pluripotent Stem Cells.
Avian species are among the most diverse vertebrates on our planet and significantly contribute to the balance of the ecology. They are also important food source and serve as a central animal model to decipher developmental biology and disease principles. Derivation of induced pluripotent stem cells (iPSCs) from avian species would enable conservation of genetic diversity as well as offer a valuable cell source that facilitates the use of avian models in many areas of basic and applied research. In this chapter, we describe methods used to successfully reprogram quail fibroblasts into iPSCs by using human transcription factors and the techniques critical to the characterization of their pluripotency.
['Lu|Yangqing|Y|', 'West|Franklin D|FD|', 'Jordan|Brian J|BJ|', 'Beckstead|Robert B|RB|', 'Jordan|Erin T|ET|', 'Stice|Steven L|SL|']
D000818:Animals D018929:Cell Culture Techniques D002454:Cell Differentiation D065150:Cellular Reprogramming D058732:Embryoid Bodies D005347:Fibroblasts D005822:Genetic Vectors D007150:Immunohistochemistry D057026:Induced Pluripotent Stem Cells D011784:Quail D014157:Transcription Factors D014161:Transduction, Genetic D019076:Transgenes
Avian Induced pluripotent stem cell Quail