Dendrimer based theranostic nanostructures for combined chemo- and photothermal therapy of liver cancer cells in vitro.
Here we report the synthesis of multifunctional nanocarriers based on PAMAM dendrimers generation (G) 4.0, 5.0 and 6.0 fixed to polydopamine (PDA) coated magnetite nanoparticles (Fe3O4). Synthesized nanoplatforms were characterized by transmission electron microscopy (TEM), the electrokinetic (zeta) potential, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and magnetic resonance imaging (MRI). Further, we show as a proof of concept that nanocarriers functionalized with G 5.0 could be successfully applied in combined chemo- and photothermal therapy (CT-PTT) of the liver cancer cells. The cooperative effect of the modalities mentioned above led to higher mortality of cancer cells when compared to their individual performance. Moreover, the performed in vitro studies revealed that the application of dual therapy triggered the desired cell death mechanism-apoptosis. Furthermore, performed tests using Magnetic Resonance Imaging (MRI) showed that our materials have competitive contrast properties. Overall, the functionality of dendrimers has been extended by merging them with magnetic nanoparticles resulting in multifunctional hybrid nanostructures that are promising smart drug delivery system for cancer therapy.
['Jędrzak|Artur|A|', 'Grześkowiak|Bartosz F|BF|', 'Coy|Emerson|E|', 'Wojnarowicz|Jacek|J|', 'Szutkowski|Kosma|K|', 'Jurga|Stefan|S|', 'Jesionowski|Teofil|T|', 'Mrówczyński|Radosław|R|']
D000903:Antibiotics, Antineoplastic D017209:Apoptosis D049109:Cell Proliferation D003287:Contrast Media D050091:Dendrimers D004317:Doxorubicin D004339:Drug Compounding D016503:Drug Delivery Systems D065546:Drug Liberation D052203:Ferrosoferric Oxide D056945:Hep G2 Cells D006801:Humans D007211:Indoles D007259:Infrared Rays D007700:Kinetics D008279:Magnetic Resonance Imaging D058185:Magnetite Nanoparticles D010789:Phototherapy D011108:Polymers D000068936:Theranostic Nanomedicine
Photothermal therapy PAMAM dendrimers Polydopamine Drug delivery Doxorubicin Nanomedicine