Evaluation of Viral Genome Copies Within Viral Factories on Different DNA Viruses.
This article describes a simple method of measuring the number of viral genomes within viral factories. For this purpose, we use three DNA viruses replicating in the cytoplasm of the infected cells: wild-type African swine fever virus (ASFV)-Georgia 2007, culture-adapted type ASFV-BA71V, and Vaccinia virus (VV). The measurements are conducted in three steps. In the first step, after DNA staining, we evaluate Integrated Optical Density (IOD) of total DNA for each viral factory. The second step involves the calculations of the mass of DNA in the viral factories in picograms (pg). And, in the third step, by dividing the mass of DNA within viral factory by the weight of a single viral genome, we obtain the number of viral genomes within the factory.
['Karalyan|Zaven A|ZA|', 'Izmailyan|Roza A|RA|', 'Abroyan|Liana O|LO|', 'Avetisyan|Aida S|AS|', 'Hakobyan|Lina A|LA|', 'Zakaryan|Hovakim S|HS|', 'Karalova|Elena M|EM|']
D000357:African Swine Fever D000358:African Swine Fever Virus D000818:Animals D002478:Cells, Cultured D004266:DNA Virus Infections D004267:DNA Viruses D004279:DNA, Viral D016679:Genome, Viral D006367:HeLa Cells D006801:Humans D019044:Image Cytometry D013194:Staining and Labeling D013552:Swine D014615:Vaccinia D014616:Vaccinia virus
viral factory DNA virus Feulgen staining virus quantification