A novel aggregation-induced emission fluorescent probe for nucleic acid detection and its applications in cell imaging.
A new kind of aggregation-induced emission compound was synthesized and used as the probe of nucleic acid. The characterization of this compound was studied. Both the RNA and DNA were detected by using this probe. And the detection scope of DNA and RNA was different. We researched the selectivity of our probe in double and single strand DNA sequences. The visualization of gel electrophoresis and the cell nucleus imaging were researched as well. Compared with the traditional nucleus dye Hoechst 33258, our probe also has the potential to be nucleus dye. And the cell toxicity was well performed by MTT assays.
['Xu|Xiaowei|X|', 'Yan|Shengyong|S|', 'Zhou|Yimin|Y|', 'Huang|Rong|R|', 'Chen|Yuqi|Y|', 'Wang|Jiaqi|J|', 'Weng|Xiaocheng|X|', 'Zhou|Xiang|X|']
D002467:Cell Nucleus D004247:DNA D005456:Fluorescent Dyes D006367:HeLa Cells D006801:Humans D057054:Molecular Imaging D015394:Molecular Structure D012313:RNA D013050:Spectrometry, Fluorescence
Aggregation-induced emission Fluorescent Nucleic acid Cell imaging Gel electrophoresis Dye