Advanced Glycation End Products Modulate Amyloidogenic APP Processing and Tau Phosphorylation: A Mechanistic Link between Glycation and the Development of Alzheimer's Disease.
Advanced glycation end products (AGEs) are implicated in the pathology of Alzheimer's disease (AD), as they induce neurodegeneration following interaction with the receptor for AGE (RAGE). This study aimed to establish a mechanistic link between AGE-RAGE signaling and AD pathology. AGE-induced changes in the neuro2a proteome were monitored by SWATH-MS. Western blotting and cell-based reporter assays were used to investigate AGE-RAGE regulated APP processing and tau phosphorylation in primary cortical neurons. Selected protein expression was validated in brain samples affected by AD. The AGE-RAGE axis altered proteome included increased expression of cathepsin B and asparagine endopeptidase (AEP), which mediated an increase in Aβ1-42 formation and tau phosphorylation, respectively. Elevated cathepsin B, AEP, RAGE, and pTau levels were found in human AD brain, coincident with enhanced AGEs. This study demonstrates that the AGE-RAGE axis regulates Aβ1-42 formation and tau phosphorylation via increased cathepsin B and AEP, providing a new molecular link between AGEs and AD pathology.
['Batkulwar|Kedar|K|', 'Godbole|Rashmi|R|', 'Banarjee|Reema|R|', 'Kassaar|Omar|O|', 'Williams|Robert J|RJ|', 'Kulkarni|Mahesh J|MJ|0000-0003-3932-9092']
D000544:Alzheimer Disease D016229:Amyloid beta-Peptides D000818:Animals D001921:Brain D017127:Glycation End Products, Advanced D051379:Mice D009474:Neurons D010766:Phosphorylation D000067759:Receptor for Advanced Glycation End Products D015398:Signal Transduction D016875:tau Proteins
Advanced glycation end products Tau phosphorylation Proteomics cathepsin B Asparagine endopeptidase Alzheimer’s disease Amyloid beta Diabetes