Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes.
Brazilian Northeast is the world's largest producer of Agave sisalana Perrine for the supply of the sisal fiber. About 95% of plant biomass, which comprise the mucilage and sisal juice, is considered a waste residual is discarded in the soil. However, the sisal juice is rich in steroidal saponins, which exhibits different pharmacological properties. Despite this, natural products are not necessarily safe. Based on this, this study analyzed the antioxidant, cytotoxic and mutagenic potential of three extracts derived from acid hydrolysis (AHAS), dried precipitate (DPAS) and hexanic of A. sisalana (HAS). These analyses were performed by in vitro and in vivo methods, using Vero cells, human lymphocytes and mice. Results showed that AHAS 50 and 100 can be considered a useful antineoplastic candidate due to their antioxidant and cytotoxic activity, with no genotoxic/clastogenic potential in Vero cells and mice. Although the comet assay in human lymphocytes has showed that the AHAS 25, AHAS 50 and AHAS 100 can lead to DNA breaks, these extracts did not promote DNA damages in mice bone marrow. Considering the different mutagenic responses obtained with the different methods employed, this study suggest that the metabolizing pathways can produce by-products harmful to health. For this reason, it is mandatory to analyze the mutagenic potential by both in vitro and in vivo techniques, using cells derived from different species and origins.
['Araldi|Rodrigo Pinheiro|RP|', 'Dos Santos|Maristela Oliveira|MO|', 'Barbon|Fabiane Faria|FF|', 'Manjerona|Bruna Aparecida|BA|', 'Meirelles|Bruno Rosa|BR|', 'de Oliva Neto|Pedro|P|', 'da Silva|Pedro Ismael|PI|', 'Dos Santos|Lucinéia|L|', 'Camargo|Isabel Cristina Cherici|ICC|', 'de Souza|Edislane Barreiros|EB|']
D027882:Agave D000818:Animals D017304:Annexin A5 D000975:Antioxidants D016923:Cell Death D002522:Chlorocebus aethiops D002853:Chromatography, Liquid D020552:Comet Assay D053903:DNA Breaks, Double-Stranded D004912:Erythrocytes D005452:Fluoresceins D006657:Histones D006801:Humans D008214:Lymphocytes D013058:Mass Spectrometry D051379:Mice D016296:Mutagenesis D010936:Plant Extracts D018515:Plant Leaves D011419:Propidium D012503:Saponins D014709:Vero Cells
Agave sisalana Comet assay Histone ?-H2AX assay Micronucleus test Mutagenesis