Paenibacillus bryophyllum sp. nov., a nitrogen-fixing species isolated from Bryophyllum pinnatum.
A nitrogen-fixing, endospore-forming bacterium, designated strain L201T was isolated from the leaves of Bryophyllum pinnatum growing in South China Agricultural University. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain L201T is affiliated with the genus Paenibacillus, and closely related to Paenibacillus albidus Q4-3T (97.4%), Paenibacillus odorifer DSM 15391T (97.3%) and Paenibacillus borealis DSM 13188T (97.2%). The main fatty acids components was anteiso-C15:0 (48.1%). The predominant isoprenoid quinone was MK-7. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The G+C content of strain L201T was 43.9%. DNA-DNA relatedness between L201T and the reference strain was 29.8%. Biological and biochemical tests, protein patterns, genomic DNA fingerprinting and comparison of cellular fatty acids distinguished strain L201T from the closely related Paenibacillus species. Based on these data, the novel species Paenibacillus bryophyllum sp. nov. is proposed, with the type strain L201T(= KCTC 33951 T = GDMCC 1.1251 T).
['Liu|Lihui|L|', 'Yuan|Tao|T|', 'Yang|Fei|F|', 'Liu|Ziwei|Z|', 'Yang|Meiyan|M|', 'Peng|Guixiang|G|', 'Tan|Zhiyuan|Z|']
D001482:Base Composition D016172:DNA Fingerprinting D057075:Enzyme Assays D005227:Fatty Acids D015870:Gene Expression D031962:Kalanchoe D007700:Kinetics D009586:Nitrogen Fixation D009591:Nitrogenase D056507:Paenibacillus D010743:Phospholipids D010802:Phylogeny D018515:Plant Leaves D012329:RNA, Bacterial D012336:RNA, Ribosomal, 16S D013729:Terpenes
Paenibacillus bryophyllum sp. nov. Nitrogen-fixing Bryophyllum pinnatum Novel species