Application of cyclodextrinase in non-complexant production of γ-cyclodextrin.
The production of γ-cyclodextrin usually includes the utilization of organic complexants. However, the non-complexant production of γ-cyclodextrin is always being explored due to the defects of organic complexants. However, in non-complexant production, the separation of γ-cyclodextrin from α- and β-cyclodextrin is still a challenge. Here, the selective hydrolysis ability of a cyclodextrinase designated PpCD (cyclodextrinase from Palaeococcus pacificus) on α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin was proved. The kcat /Km values of PpCD for α-cyclodextrin and β-cyclodextrin were roughly 12-fold and 5-fold higher than that of γ-cyclodextrin. It was proved that PpCD had selective hydrolysis ability and its γ-cyclodextrin purification performance was apparent on various simulated cyclodextrin mixtures with reported proportions derived from different CGTases. Besides, the hydrolysis temperature was optimized and it could be seen that 85°C was appropriate for the production of γ-cyclodextrin. In addition, the production of γ-cyclodextrin was achieved by using PpCD in the γ-CGTase reaction products.
['Ji|Hangyan|H|', 'Wang|Yu|Y|', 'Bai|Yuxiang|Y|', 'Li|Xiaoxiao|X|', 'Qiu|Lizhong|L|', 'Jin|Zhengyu|Z|0000-0001-6880-9955']
D001407:Bacillus D003124:Colorimetry D006026:Glycoside Hydrolases D006868:Hydrolysis D013696:Temperature D047408:gamma-Cyclodextrins
cyclodextrinase non-complexant γ-Cyclodextrin selective hydrolysis