Fractionation of Subcellular Organelles.
This unit provides both a theoretical and a practical background to all the techniques associated with the application of differential and density gradient centrifugation for the analysis of subcellular membranes. The density gradient information focuses on the use of the modern gradient solute iodixanol, chosen for its ease of use, versatility, and compatibility with biological particles. Its use in both pre-formed discontinuous and continuous gradients and in self-generated gradients is discussed. Considerable emphasis is given to selection of the appropriate centrifuge rotors and tubes and their influence on the methods used for creation, fractionation, and analysis of density gradients. Without proper consideration of these critical ancillary procedures, the resolving power of the gradient can be easily compromised.
['Graham|John M|JM|']
D000818:Animals D002458:Cell Fractionation D002499:Centrifugation, Density Gradient D006801:Humans D015388:Organelles D013347:Subcellular Fractions D014283:Triiodobenzoic Acids
density gradient technology differential centrifugation instrumentation