Steroidobacter soli sp. nov., isolated from farmland soil.
A Gram-stain-negative bacterial strain, designated JW-3T, was isolated from a soil sample collected from farmland in Yantai, Shandong Province, PR China. Cells of strain JW-3T are motile rods and strictly aerobic, showing catalase- and oxidase-positive reactions. Strain JW-3T could grow at 16-37 °C (optimum, 30 °C), at pH 6.0-9.0 (pH 7.0) and in the presence of 0-1 % (w/v) NaCl (0.5 %, in Luria-Bertani broth). The major fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 35.5 %), iso-C16 : 0 (16.7 %) and C12 : 0 (10.8 %). The major respiratory quinone was ubiquinone-8 (Q8). The polar lipids of strain JW-3T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified phospholipids, two unidentified lipids, two unidentified glycolipids and a partial unidentified aminophospholipid. Strain JW-3T was most closely related to Steroidobacter agariperforans KA5-BT with 97.67 % 16S rRNA gene sequence similarity. Results of phylogenetic analyses, based on 16S rRNA gene sequencing, showed that strain JW-3T forms a distinct phylogenic lineage within the genus Steroidobacter of the family Sinobacteraceae. The DNA G+C content of strain JW-3T was 62.57 mol%, based on its draft genome sequence. Average nucleotide identity values and digital DNA-DNA hybridization values for draft genomes, between strain JW-3T and strain KA5-BT, were 84.54 and 30.80 %, respectively. Based on its phenotypic, chemotaxonomic and molecular features, and DNA-DNA hybridization results, strain JW-3T represents a novel species of the genus Steroidobacter, for which the name Steroidobactersoli sp. nov. is proposed. The type strain is JW-3T (=CCTCC AB 2018184T=KCTC 62820T).
['Huang|Jun-Wei|JW|', 'Hu|Shun-Li|SL|', 'Cheng|Xiao-Kun|XK|', 'Chen|Dian|D|', 'Kong|Xiang-Kun|XK|', 'Jiang|Jian-Dong|JD|']
D015373:Bacterial Typing Techniques D001482:Base Composition D002681:China D004269:DNA, Bacterial D000072480:Farms D005227:Fatty Acids D020563:Gammaproteobacteria D006017:Glycolipids D009693:Nucleic Acid Hybridization D010743:Phospholipids D010802:Phylogeny D012336:RNA, Ribosomal, 16S D017422:Sequence Analysis, DNA D012988:Soil Microbiology D014451:Ubiquinone
Steroidobacter new taxa taxonomy