Effect of tributyltin on antioxidant ability and immune responses of zebrafish (Danio rerio).
Tributyltin (TBT) is a toxic compound released into aquatic ecosystems through antifouling paints. This study was designed to examine the effects of TBT on antioxidant ability and immune responses of zebrafish (Danio rerio). Three hundred sixty healthy zebrafish were randomly grouped into four groups and exposed to different doses of TBT (0, 1, 10 and 100ngL-1). At the end of 8 weeks, the fish were sampled, and antioxidant capability, immune parameters and immune-related genes were assessed. The results showed that with an increase in TBT dose, the concentration of malonaldehyde in the liver was significantly increased (p<0.05), whereas the activities of total superoxide dismutase, catalase and glutathione peroxidase were significantly decreased (p<0.05) compared to the control. The activity and expression of lysozyme and the content of immunoglobulin M were significantly decreased compared to those of the fish exposed to 0ngL-1 TBT (p<0.05). However, the expression of the HSP70, HSP90, tumor necrosis factor-α(TNF-α), interleukins (IL-1β, IL-6), and nuclear factor-kappa B p65 (NF-κ B p65) genes were all enhanced with an increase in TBT dose. The results indicated that TBT induced oxidative stress and had immunotoxic effects on zebrafish.
['Zhang|Chun-Nuan|CN|', 'Zhang|Ji-Liang|JL|', 'Ren|Hong-Tao|HT|', 'Zhou|Bian-Hua|BH|', 'Wu|Qiu-Jue|QJ|', 'Sun|Ping|P|']
D000818:Animals D000975:Antioxidants D002374:Catalase D016207:Cytokines D004202:Disinfectants D005979:Glutathione Peroxidase D006360:Heat-Shock Proteins D007109:Immunity D008099:Liver D008315:Malondialdehyde D009113:Muramidase D018384:Oxidative Stress D011897:Random Allocation D013482:Superoxide Dismutase D014220:Trialkyltin Compounds D014409:Tumor Necrosis Factor-alpha D014874:Water Pollutants, Chemical D015027:Zebrafish
Tributyltin Immune response Danio rerio Antioxidant capability