DNA barcoding detects floral origin of Indian honey samples.
The unique medicinal and nutritional properties of honey are determined by its chemical composition. To evaluate the quality of honey, it is essential to study the surrounding vegetation where honeybees forage. In this study we used conventional melissopalynological and DNA barcoding techniques to determine the floral source of honey samples collected from different districts of the state of Mizoram, India. Pollen grains were isolated and genomic DNA was extracted from the honey samples. PCR amplification was carried out using universal barcode candidates ITS2 and rbcL to identify the plant species. Furthermore, TA cloning was carried out to screen the PCR amplicon libraries to identify the presence of multiple plant species. Results from both the melissopalynological and DNA barcoding analyses identified almost exactly the same 22 species, suggesting that both methods are suitable for analysis. However, DNA barcoding is easier and widely practiced. Hence, it can be concluded that DNA barcoding is a useful tool in determining the medicinal and commercial value of honey.
['Saravanan|Mohanasundaram|M|', 'Mohanapriya|Gunasekaran|G|', 'Laha|Ramachandra|R|', 'Sathishkumar|Ramalingam|R|']
D000818:Animals D001516:Bees D058893:DNA Barcoding, Taxonomic D021901:DNA, Intergenic D018744:DNA, Plant D035264:Flowers D006722:Honey D007194:India D010944:Plants D011058:Pollen D016133:Polymerase Chain Reaction D012273:Ribulose-Bisphosphate Carboxylase
DNA barcoding honey melissopalynology codage à barres de l’ADN composition florale floral composition miel mélissopalynologie