Novel biochip platform for nucleic acid analysis.
This manuscript describes the use of a novel biochip platform for the rapid analysis/identification of nucleic acids, including DNA and microRNAs, with very high specificity. This approach combines a unique dynamic chemistry approach for nucleic acid testing and analysis developed by DestiNA Genomics with the STMicroelectronics In-Check platform, which comprises two microfluidic optimized and independent PCR reaction chambers, and a sequential microarray area for nucleic acid capture and identification by fluorescence. With its compact bench-top "footprint" requiring only a single technician to operate, the biochip system promises to transform and expand routine clinical diagnostic testing and screening for genetic diseases, cancers, drug toxicology and heart disease, as well as employment in the emerging companion diagnostics market.
['Pernagallo|Salvatore|S|', 'Ventimiglia|Giorgio|G|', 'Cavalluzzo|Claudia|C|', 'Alessi|Enrico|E|', 'Ilyine|Hugh|H|', 'Bradley|Mark|M|', 'Diaz-Mochon|Juan J|JJ|']
D001483:Base Sequence D023281:Genomics D006801:Humans D008574:Mengovirus D035683:MicroRNAs D046210:Microfluidic Analytical Techniques D008969:Molecular Sequence Data D015341:Nucleic Acid Probes D009696:Nucleic Acids D009841:Oligonucleotides D012367:RNA, Viral D015203:Reproducibility of Results
nucleic acid dynamic chemistry microfluidic microarray Lab-on-Chip (LoC) mengo virus (MGV) microRNA-122 (miRNA122) nucleic acid test (NAT) peptide nucleic acid (PNA) point-of-care (POC) polymerase chain reaction (PCR) single nucleotide polymorphism (SNP)