Supplementation of the BIOXcell extender with the antioxidants crocin, curcumin and GSH for freezing bull semen.
Semen cryopreservation is routine in cattle, but the results of artificial insemination need improvement. A strategy to these aims is the supplementation of the freezing extender with novel antioxidants. This study aimed at testing the natural antioxidants curcumin and crocin as supplements to the commercial extender BIOXcell for freezing semen from 8 Holstein bulls. We tested curcumin at 0.05 and 0.1 mM (CU0.05, CU0.1) and crocin at 0.5 and 1.5 mM (CR0.5, CR1.5), with 0.5 mM reduced glutathione (GSH0.5) as reference, and a control (CTL, without supplementation). The samples were evaluated post-thawing and after 5 h at 38 °C by CASA for motility and flow cytometry for viability, apoptotic, capacitation, acrosomal status, cytoplasmic and mitochondrial reactive oxygen species (ROS) production, and chromatin status (SCSA). Control and GSH0.5 showed similar results, possibly because of the good protection from BIOXcell. CU0.05 and CU0.1 showed little effects but increased cytoplasmic ROS production and motility ALH. CR0.5 and CR1.5 decreased viability and increased apoptotic features significantly post-thawing and after the incubation, resulting in lower motility (significant after the incubation) but decreasing SCSA %HDS (loose chromatin). Whereas crocin at these concentrations seems incompatible with BIOXcell, maybe because of a prooxidant activity, curcumin use merits further research, considering the elevation of ROS with no significant negative effects.
['Salman|Amer|A|', 'Caamaño|J Néstor|JN|', 'Fernández-Alegre|Estela|E|', 'Hidalgo|Carlos O|CO|', 'Nadri|Touba|T|', 'Tamargo|Carolina|C|', 'Fueyo|Carmen|C|', 'Fernández|Ángel|Á|', 'Merino|María J|MJ|', 'Martínez-Pastor|Felipe|F|']
D000818:Animals D000975:Antioxidants D002338:Carotenoids D002417:Cattle D015925:Cryopreservation D003470:Culture Media D003474:Curcumin D005978:Glutathione D008297:Male D012661:Semen D012662:Semen Preservation D013081:Sperm Motility
Crocin Curcumin Cryopreservation Cattle ROS Spermatozoa