In Vitro Differentiation of T Cells from Murine Pluripotent Stem Cells.
In recent years cancer immunotherapy, especially the cell-based immunotherapy, has reached several milestones and achieved a lot of cancer remission in the clinics. Obtaining a more potent and effective cytotoxic T lymphocytes (CTLs) for cancer immunotherapy is always the ultimate goal for the researchers. However, the difficulty in harvesting a large number of tumor antigen-specific CTLs from the tumor patient is still a major obstacle we need to overcome. In our previous studies, it is shown that pluripotent stem cell-derived CTL-especially the genetically engineered antigen-specific CTLs-may serve as a good source of unlimited number of highly reactive and antigen-specific CTLs. Here we present a two-step method for the generation of antigen-specific T lymphocytes from iPS cells by in vitro priming and in vivo maturation.
['Chen|Xiaoniao|X|', 'Lei|Fengyang|F|', 'Wang|Liqiang|L|', 'Xiong|Xiaofang|X|', 'Song|Jianxun|J|']
D000818:Animals D000951:Antigens, Neoplasm D002135:Calcium-Binding Proteins D018929:Cell Culture Techniques D002454:Cell Differentiation D045744:Cell Line, Tumor D018920:Coculture Techniques D003470:Culture Media D004195:Disease Models, Animal D005260:Female D005434:Flow Cytometry D005822:Genetic Vectors D018398:Homeodomain Proteins D006801:Humans D016219:Immunotherapy, Adoptive D057026:Induced Pluripotent Stem Cells D008546:Melanoma, Experimental D051379:Mice D018345:Mice, Knockout D015202:Protein Engineering D011948:Receptors, Antigen, T-Cell D011994:Recombinant Proteins D012190:Retroviridae D013602:T-Lymphocytes, Cytotoxic D014161:Transduction, Genetic
Differentiation T cells Induced pluripotent stem cells Notch signaling Stem cell biology