Potentiation of antigen-specific antibody production by peptides derived from Ag85B of Mycobacterium tuberculosis.
To generate high-titer monoclonal antibodies, strong immuno-stimulation must be used for eliciting an intense cellular immune response. Here, we report that antigen-specific antibody production was potentiated by Peptide-25 derived from Ag85B of Mycobacterium tuberculosis, and that the production of antigen-specific IgG1 in particular was markedly potentiated; specifically, this occurred because the use of Peptide-25 resulted in an increase in the number of antigen-specific antibody-producing cells. We studied the activation of T cells by the peptide by examining gene expression. The observed expression pattern of GATA-3 and T-bet suggests that the peptide modulates the Th1/Th2 balance during immunization. This potentiation, which was remarkably high in BALB/c mice, could be applied in the immunization performed for monoclonal antibody production in vivo and in vitro.
['Kato|Mieko|M|', 'Hanyu|Yoshiro|Y|']
D000217:Acyltransferases D000276:Adjuvants, Immunologic D000818:Animals D000911:Antibodies, Monoclonal D000921:Antibody-Producing Cells D000942:Antigens, Bacterial D001426:Bacterial Proteins D005260:Female D050990:GATA3 Transcription Factor D015870:Gene Expression D007074:Immunoglobulin G D051379:Mice D008807:Mice, Inbred BALB C D008809:Mice, Inbred C3H D008810:Mice, Inbred C57BL D009169:Mycobacterium tuberculosis D010446:Peptide Fragments D020825:T-Box Domain Proteins D058408:Th1-Th2 Balance
Antibody Immunization Peptide-25 Th2 Adjuvant MHC-II