Antioxidant activity of proanthocyanidins-rich fractions from Choerospondias axillaris peels using a combination of chemical-based methods and cellular-based assay.
An extract isolated from Choerospondias axillaris peels was separated into five fractions using size-exclusion chromatography. The structural composition and mean degree of polymerization (mDP) of these fractions were then characterized by acid-catalysis followed by HPLC analysis. The antioxidant activity of each fraction was determined using a combination of chemical-based methods (DPPH, ABTS(+) radical scavenging activity, ferric-reducing antioxidant power, and phosphomolybdate assay) and a cellular-based assay. All fractions tested were found to have high total phenolics contents and were rich in proanthocyanidins. The mDP of fractions (F1-F5) ranged from 1.92 to 9.25. When tested by the chemical-based assays, the antioxidant activity of the fractions did not depend on molecular weight of the phenolics. Conversely, when tested by the cellular-based assay the antioxidant activity actually decreased with increasing molecular weight of the proanthocyanidins. These experiments highlight the limitations of using chemical-based assays to establish the antioxidant activity of proanthocyanidins within biological systems.
['Li|Qian|Q|', 'Wang|Xieyi|X|', 'Chen|Jun|J|', 'Liu|Chengmei|C|', 'Li|Ti|T|', 'McClements|David Julian|DJ|', 'Dai|Taotao|T|', 'Liu|Jiyan|J|']
D027926:Anacardiaceae D000975:Antioxidants D049109:Cell Proliferation D002478:Cells, Cultured D002851:Chromatography, High Pressure Liquid D005638:Fruit D061307:Human Umbilical Vein Endothelial Cells D010084:Oxidation-Reduction D010636:Phenols D010936:Plant Extracts D044945:Proanthocyanidins
Antioxidants Choerospondias axillaris Proanthocyanidins Cellular-based methods Nutraceuticals Structure-activity