Identification of dysregulation of atrial proteins in rats with chronic obstructive apnea using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry.
Obstructive sleep apnea (OSA) affects an estimated 20% of adults worldwide and has been associated with electrical and structural abnormalities of the atria, although the molecular mechanisms are not well understood. Here, we used two-dimensional polyacrylamide gel electrophoresis (2D PAGE) coupled with nanoliquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) to investigate the proteins that are dysregulated in the atria from severe and moderate apnea when compared to control. We found enzymes involved in the glycolysis, beta-oxidation, electron transport chain and Krebs cycle to be down-regulated. The data suggested that the dysregulated proteins may play a role in atrial pathology developing via chronic obstructive apnea and hypoxia. Our results are consistent with our previous 1D-PAGE and nanoLC-MS/MS study (Channaveerappa et al, J Cell Mol Med. 2017), where we found that some aerobic and anaerobic glycolytic and Krebs cycle enzymes were down-regulated, suggesting that apnea may be a result of paucity of oxygen and production of ATP and reducing equivalents (NADH). The 2D-PAGE study not only complements our current study, but also advances our understanding of the OSA. The complete mass spectrometry data are available via ProteomeXchange with identifier PXD011181.
['Lux|Jacob C|JC|', 'Channaveerappa|Devika|D|', 'Aslebagh|Roshanak|R|', 'Heintz|Timothy A|TA|', 'McLerie|Meredith|M|', 'Panama|Brian K|BK|0000-0003-4135-6671', 'Darie|Costel C|CC|0000-0001-6402-2311']
D000818:Animals D015180:Electrophoresis, Gel, Two-Dimensional D006325:Heart Atria D006331:Heart Diseases D009124:Muscle Proteins D020543:Proteome D051381:Rats D020181:Sleep Apnea, Obstructive D053719:Tandem Mass Spectrometry
rat apnea hypoxia animal proteomics cardiovascular system metabolism two-dimensional electrophoresis