Pattern recognition-based optical technique for non-destructive detection of Ectomyelois ceratoniae infestation in pomegranates during hidden activity of the larvae.
In this research, the feasibility of utilizing visible/near-infrared (Vis/NIR) spectroscopy as an optical non-destructive technique combined with both supervised and unsupervised pattern recognition methods was assessed for detection of Ectomyelois ceratoniae, carob moth, infestation in pomegranates during hidden activity of the larvae. To this end, some fruits were artificially contaminated to the carob moth larvae. Vis/NIR spectra of the blank samples and the contaminated pomegranates without and with external visual symptoms of larvae infestation were analyzed one and two weeks after contaminating the samples as three groups of "Healthy", "Unhealthy-A" and "Unhealthy-B", respectively. Principal component analysis (PCA) as unsupervised pattern recognition method was used to verify the possibility of clustering of the pomegranate samples into the three groups. Discriminant analysis (DA) based on PCA was also used as a powerful supervised pattern recognition method to classify the samples. The calibration models of linear, quadratic and Mahalanobis discriminant analyses were developed based on different spectral pre-processing techniques. The best PCA-DA model was obtained using Mahalanobis distance method and first derivative (D1) pre-processing. The total percentage of correctly classified samples with the best calibration model was 97.9%. The developed model could also predict unknown samples with total percentage of correctly classified samples of 90.6%. It was concluded that Vis/NIR spectroscopy combined with pattern recognition method of PCA-DA can be an appropriate and rapid technology for non-destructively screening the pomegranates for detection of carob moth infestation during hidden activity of the larvae.
['Jamshidi|Bahareh|B|', 'Mohajerani|Ezeddin|E|', 'Farazmand|Hossein|H|', 'Mahmoudi|Asghar|A|', 'Hemmati|Abolfazl|A|']
D000818:Animals D016002:Discriminant Analysis D005504:Food Analysis D005638:Fruit D007814:Larva D029561:Lythraceae D009036:Moths D010363:Pattern Recognition, Automated D010935:Plant Diseases D025341:Principal Component Analysis D019265:Spectroscopy, Near-Infrared
Non-destructive Pomegranate Near-infrared Spectroscopy Carob moth Discriminant analysis Insect infestation