Effect of quercetin on nonshivering thermogenesis of brown adipose tissue in high-fat diet-induced obese mice.
Activating nonshivering thermogenesis in brown adipose tissue (BAT) is a promising strategy to prevent obesity. This study investigated whether quercetin supplementation improves obesity in mice by increasing nonshivering thermogenesis in BAT and white adipose tissue (WAT) browning. Compared to high-fat diet (HFD)-fed mice, mice fed a HFD supplemented with 1% quercetin (HFDQ) had reduced body weight and total plasma cholesterol. In HFDQ-fed mice, retroperitoneal WAT (RWAT) weight was decreased, and browning effect and lipolysis were increased. HFDQ-fed mice had increased expression of nonshivering thermogenesis genes in BAT, including uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC1α), cell death-inducing DFFA-like effector A (CIDEA), and mitochondrial transcriptional factor A (mtTFA). Quercetin supplementation increased genes and proteins in β3-adrenergic receptor (ADRB3), p38 mitogen-activated protein kinase (MAPK), and AMP-activated protein kinase (AMPK) pathways in HFD-fed mice, which were suppressed by an AMPK inhibitor or an ADRB3 antagonist. Energy expenditure and core body temperature were not changed by quercetin, but physical activity was increased in HFDQ mice during dark periods at room and cold temperatures. Quercetin also decreased the Firmicutes to Bacteroidetes ratio and increased short-chain fatty acid production in the feces of HFD-fed mice. In summary, quercetin supplementation in HFD-fed mice may attenuate obesity. Although the study did not show consistency in data at molecular and pathophysiological levels between BAT function and obesity, it also shows promising health effects of quercetin, accompanied by improved physical activity and gut microbiota dysbiosis.
['Pei|Ya|Y|', 'Otieno|Dammah|D|', 'Gu|Inah|I|', 'Lee|Sun-Ok|SO|', 'Parks|John S|JS|', 'Schimmel|Keith|K|', 'Kang|Hye Won|HW|']
D055372:AMP-Activated Protein Kinases D002001:Adipose Tissue, Brown D052436:Adipose Tissue, White D000818:Animals D002784:Cholesterol D004268:DNA-Binding Proteins D059305:Diet, High-Fat D004734:Energy Metabolism D008066:Lipolysis D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D008820:Mice, Obese D024101:Mitochondrial Proteins D009765:Obesity D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha D011794:Quercetin D022702:Receptors, Adrenergic, beta-3 D022722:Thermogenesis D014157:Transcription Factors D000071256:Uncoupling Protein 1
quercetin brown adipose tissue uncoupling protein 1 AMP-activated protein kinase