Defined host factors support HBV infection in non-hepatic 293T cells.
Hepatitis B virus (HBV) is a human hepatotropic virus. However, HBV infection also occurs at extrahepatic sites, but the relevant host factors required for HBV infection in non-hepatic cells are only partially understood. In this article, a non-hepatic cell culture model is constructed by exogenous expression of four host genes (NTCP, HNF4α, RXRα and PPARα) in human non-hepatic 293T cells. This cell culture model supports HBV entry, transcription and replication, as evidenced by the detection of HBV pgRNA, HBV cccDNA, HBsAg, HBeAg, HBcAg and HBVDNA. Our results suggest that the above cellular factors may play a key role in HBV infection of non-hepatic cells. This model will facilitate the identification of host genes that support extrahepatic HBV infection.
['Yang|Xiaoqiang|X|', 'Cai|Weiwen|W|', 'Sun|Xiaoyue|X|', 'Bi|Yanwei|Y|', 'Zeng|Chui|C|', 'Zhao|XiaoYu|X|', 'Zhou|Qi|Q|', 'Xu|Tian|T|', 'Xie|Qingdong|Q|', 'Sun|Pingnan|P|0000-0002-3325-8062', 'Zhou|Xiaoling|X|0000-0001-5721-944X']
D002460:Cell Line D045744:Cell Line, Tumor D004279:DNA, Viral D057809:HEK293 Cells D056945:Hep G2 Cells D006509:Hepatitis B D006514:Hepatitis B Surface Antigens D006513:Hepatitis B e Antigens D006515:Hepatitis B virus D022781:Hepatocytes D006801:Humans D014779:Virus Replication
HBV non-hepatic cells NTCP HNF4α RXRα PPARα nuclear hormone receptors