Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.
Background
Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide. Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.


Purpose
To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.


Methods
CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering. Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC. Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods. The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.


Results
The release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner. CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes. In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone. Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition. CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.


Conclusion
The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.
['Sun|Tongyi|T|', 'Zhan|Bo|B|', 'Zhang|Weifen|W|', 'Qin|Di|D|', 'Xia|Guixue|G|', 'Zhang|Huijie|H|', 'Peng|Meiyu|M|', 'Li|Sheng-An|SA|', 'Zhang|Yun|Y|', 'Gao|Yuanyuan|Y|', 'Lee|Wen-Hui|WH|']
D000818:Animals D000900:Anti-Bacterial Agents D002465:Cell Movement D048271:Chitosan D002921:Cicatrix D003094:Collagen D016207:Cytokines D016503:Drug Delivery Systems D065546:Drug Liberation D000067493:Dynamic Light Scattering D004705:Endocytosis D005260:Female D006801:Humans D015603:Keratinocytes D051379:Mice D008819:Mice, Nude D008826:Microbial Sensitivity Tests D053758:Nanoparticles D010455:Peptides D000067996:RAW 264.7 Cells D014945:Wound Healing
nanoparticles OH-CATH30 wound healing antimicrobial peptide skin destruction