Poly-ϵ-caprolactone/chitosan nanoparticles provide strong adjuvant effect for hepatitis B antigen.
AIM
This work aims to investigate the adjuvant effect of poly-ϵ-caprolactone/chitosan nanoparticles (NPs) for hepatitis B surface antigen (HBsAg) and the plasmid DNA encoding HBsAg (pRC/CMV-HBs).


METHODS
Both antigens were adsorbed onto preformed NPs. Vaccination studies were performed in C57BL/6 mice. Transfection efficiency was investigated in A549 cell line.


RESULTS
HBsAg-adsorbed NPs generated strong anti-HBsAg IgG titers, mainly of IgG1 isotype, and induced antigen-specific IFN-γ and IL-17 secretion by spleen cells. The addition of pRC/CMV-HBs to the HBsAg-adsorbed NPs inhibited IL-17 secretion but had minor effect on IFN-γ levels. Lastly, pRC/CMV-HBs-loaded NPs generated a weak serum antibody response.


CONCLUSION
Poly-ϵ-caprolactone/chitosan NPs provide a strong humoral adjuvant effect for HBsAg and induce a Th1/Th17-mediated cellular immune responses worth explore for hepatitis B virus vaccination.
['Jesus|Sandra|S|', 'Soares|Edna|E|', 'Borchard|Gerrit|G|', 'Borges|Olga|O|']
D000072283:A549 Cells D000276:Adjuvants, Immunologic D000281:Administration, Intranasal D000818:Animals D000917:Antibody Formation D002626:Chemistry, Pharmaceutical D048271:Chitosan D004337:Drug Carriers D005260:Female D006514:Hepatitis B Surface Antigens D006801:Humans D007111:Immunity, Cellular D007074:Immunoglobulin G D020381:Interleukin-17 D008810:Mice, Inbred C57BL D053758:Nanoparticles D010316:Particle Size D011091:Polyesters D013154:Spleen D013499:Surface Properties D014162:Transfection
hepatitis B HBsAg pDNA vaccine poly-ϵ-caprolactone (PCL)/chitosan nanoparticles vaccine adjuvants