Evaluation of a temperature-restricted, mucosal tuberculosis vaccine in guinea pigs.
Tuberculosis (TB) is currently the leading cause of death in humans by a single infectious agent, Mycobacterium tuberculosis. The Bacillus Calmette-Guérin (BCG) vaccine prevents pulmonary TB with variable efficacy, but can cause life-threatening systemic infection in HIV-infected infants. In this study, TBvac85, a derivative of Mycobacterium shottsii expressing M. tuberculosis Antigen 85B, was examined as a safer alternative to BCG. Intranasal vaccination of guinea pigs with TBvac85, a naturally temperature-restricted species, resulted in serum Ag85B-specific IgG antibodies. Delivery of the vaccine by this route also induced protection equivalent to intradermal BCG based on organ bacterial burdens and lung pathology six weeks after aerosol challenge with M. tuberculosis strain Erdman. These results support the potential of TBvac85 as the basis of an effective TB vaccine. Next-generation derivatives expressing multiple M. tuberculosis immunogens are in development.
['Gupta|Tuhina|T|', 'LaGatta|Monica|M|', 'Helms|Shelly|S|', 'Pavlicek|Rebecca L|RL|', 'Owino|Simon O|SO|', 'Sakamoto|Kaori|K|', 'Nagy|Tamas|T|', 'Harvey|Stephen B|SB|', 'Papania|Mark|M|', 'Ledden|Stephanie|S|', 'Schultz|Kevin T|KT|', 'McCombs|Candace|C|', 'Quinn|Frederick D|FD|', 'Karls|Russell K|RK|']
D000217:Acyltransferases D000281:Administration, Intranasal D000336:Aerosols D000818:Animals D000907:Antibodies, Bacterial D000942:Antigens, Bacterial D001426:Bacterial Proteins D004195:Disease Models, Animal D005260:Female D006168:Guinea Pigs D018928:Immunity, Mucosal D000071497:Immunogenicity, Vaccine D007074:Immunoglobulin G D008168:Lung D009169:Mycobacterium tuberculosis D009297:Nasal Mucosa D013696:Temperature D013997:Time Factors D032581:Tuberculosis Vaccines D014397:Tuberculosis, Pulmonary D014611:Vaccination D019444:Vaccines, DNA
Tuberculosis Vaccine Mycobacterium TBvac85 Intranasal