Immobilization of hyaluronic acid on plasma-sprayed porous titanium coatings for improving biological properties.
In the present study, hyaluronic acid (HyA) was covalently immobilized onto titanium coatings to improve their biological properties. Diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were employed to characterize the HyA-modified titanium coating. HyA-modified titanium coatings possess better cell-material interaction, and human mesenchymal stem cells present good adhesive morphologies on the surface of TC-AAH. The results of subsequent cellular evaluation showed that the immobilization of HyA on titanium coatings could improve hMSC attachment, proliferation, and differentiation. In vivo evaluation of implants in rabbit femur condyle defect model showed improvements of early osseointegration and bone-to-implant contact of TC-AAH. In conclusion, immobilization of HyA could improve biological properties of titanium coatings.
['Ao|Haiyong|H|', 'Xie|Youtao|Y|', 'Qin|An|A|', 'Ji|Heng|H|', 'Yang|Shengbing|S|', 'Huang|Liping|L|', 'Zheng|Xuebin|X|', 'Tang|Tingting|T|']
D000818:Animals D002448:Cell Adhesion D002454:Cell Differentiation D049109:Cell Proliferation D002478:Cells, Cultured D020099:Coated Materials, Biocompatible D005269:Femur D006801:Humans D006820:Hyaluronic Acid D008297:Male D059630:Mesenchymal Stem Cells D018613:Microscopy, Confocal D016348:Osseointegration D056951:Photoelectron Spectroscopy D016062:Porosity D011817:Rabbits D017550:Spectroscopy, Fourier Transform Infrared D014025:Titanium
hyaluronic acid titanium coating biological properties covalent immobilization osteointegration