Facile Synthesis of Folic Acid-Modified Iron Oxide Nanoparticles for Targeted MR Imaging in Pulmonary Tumor Xenografts.
PURPOSE
The purpose of this study was to develop folic acid (FA)-modified iron oxide (Fe3O4) nanoparticles (NPs) for targeted magnetic resonance imaging (MRI) of H460 lung carcinoma cells.


PROCEDURES
Water-dispersible Fe3O4 NPs synthesized via a mild reduction method were conjugated with FA to generate FA-targeted Fe3O4 NPs. The specificity of FA-targeted Fe3O4 NPs to bind FA receptor was investigated in vitro by cellular uptake and cell MRI and in vivo by MRI of H460 tumors.


RESULTS
The formed NPs displayed good biocompatibility and ultrahigh r 2 relaxivity (440.01/mM/s). The targeting effect of the NPs to H460 cells was confirmed by in vitro cellular uptake and cell MRI. H460 tumors showed a significant reduction in T2 signal intensity at 0.85 h, which then recovered and returned to control at 2.35 h.


CONCLUSIONS
The results indicate that the prepared FA-targeted Fe3O4 NPs have potential to be used as T2 negative contrast agents in targeted MRI.
['Zhang|Zaixian|Z|', 'Hu|Yong|Y|', 'Yang|Jia|J|', 'Xu|Yanhong|Y|', 'Zhang|Chengzhong|C|', 'Wang|Zhongling|Z|', 'Shi|Xiangyang|X|', 'Zhang|Guixiang|G|']
D016923:Cell Death D045744:Cell Line, Tumor D004705:Endocytosis D005290:Ferric Compounds D005492:Folic Acid D006801:Humans D008175:Lung Neoplasms D008279:Magnetic Resonance Imaging D053758:Nanoparticles D010316:Particle Size D014018:Tissue Distribution D023041:Xenograft Model Antitumor Assays
Iron oxide nanoparticles MRI FA H460 cells