Luteinizing hormone, insulin like growth factor-1, and epidermal growth factor stimulate vascular endothelial growth factor production in cultured bubaline granulosa cells.
The objective of this study was to characterize in vitro expression and secretion of vascular endothelial growth factor (VEGF) in bubaline granulosa cells (GC), grown in serum containing media supplemented with luteinizing hormone (LH), insulin like growth factor-1 (IGF-1), and epidermal growth factor (EGF) at three different doses and time durations. GCs were collected from ovarian follicles of varying diameters [Gp-I (small), 4-6 mm; Gp-II (medium), 7-9 mm; Gp-III (large), 10-13 mm; Gp-IV (pre-ovulatory), >13 mm]. In general, each of the three treatments resulted in a dose as well as time dependent increase in the mRNA expression and secretion of VEGF in the cultured GCs of Gp-IV follicles. These results were well supported by our observations on immunocytochemistry in Gp IV granulosa cell culture (GCC). We also looked into the expression dynamics of an anti-apoptotic factor--proliferating cellular antigen (PCNA) and a pro-apoptotic factor--Bcl-2-associated X protein (BAX) in GCs of Gp IV follicles on treatments with LH, IGF-1, and EGF to evaluate their cytoprotective/anti-apoptotic property. Relative expressions of PCNA and BAX showed a mutually opposite trend with the PCNA expression increasing and BAX expression decreasing with increase in dose and time to reach the zenith (P<0.05) and nadir (P<0.05) at the highest dose(s) at the maximum time duration (72 h) for PCNA and BAX respectively on treatment with all the three factors. Thus, it can be concluded that LH, IGF-1, and EGF treatments have a cytoprotective/anti-apoptotic effect and stimulate VEGF production in granulosa cells of bubaline pre-ovulatory follicles.
['Babitha|V|V|', 'Yadav|V P|VP|', 'Chouhan|V S|VS|', 'Hyder|I|I|', 'Dangi|S S|SS|', 'Gupta|Mahesh|M|', 'Khan|F A|FA|', 'Taru Sharma|G|G|', 'Sarkar|M|M|']
D000818:Animals D015153:Blotting, Western D002020:Buffaloes D004815:Epidermal Growth Factor D005260:Female D006107:Granulosa Cells D007124:Immunoenzyme Techniques D007334:Insulin-Like Growth Factor I D007986:Luteinizing Hormone D018809:Proliferating Cell Nuclear Antigen D012333:RNA, Messenger D060888:Real-Time Polymerase Chain Reaction D020133:Reverse Transcriptase Polymerase Chain Reaction D042461:Vascular Endothelial Growth Factor A
Granulosa cells VEGF LH IGF-1 EGF Buffalo