Targeted delivery of p-coumaric acid encapsulated mannosylated liposomes to the synovial macrophages inhibits osteoclast formation and bone resorption in the rheumatoid arthritis animal model.
The current study aimed to target the delivery of p-coumaric acid (CA), a dietary polyphenol to the synovial macrophages of AIA rats via mannose incorporated liposomal delivery system (ML) with reference to osteoclastogenesis and bone resorption. In vivo imaging and in vitro drug release study indicated the efficiency of mannosylated liposomes to localize at the site of inflammation and increased sustain drug release respectively. Morphological assessment of isolated synovial macrophages with respect to CD86 (synovial macrophages) and CD51 (pre-/osteoclast) indicated that p-coumaric acid encapsulated mannosylated liposomes (ML-CA) inhibited the osteoclasts differentiation. ML-CA treatment inhibited the TRAP staining, downregulated the expression of MMP-9 and NFATc1 and inflammatory cytokines. The ex-vivo study specified the ability of CA to induce the OPG production in bone marrow stromal cell triggered macrophage-osteoclasts differentiation and to preserve the calcium content. Taken together, our results demonstrated that ML-CA could intervene in the osteoclast formation.
['Neog|Manoj Kumar|MK|', 'Rasool|Mahaboobkhan|M|']
D000818:Animals D001169:Arthritis, Experimental D001172:Arthritis, Rheumatoid D051940:B7-2 Antigen D001862:Bone Resorption D002454:Cell Differentiation D003373:Coumaric Acids D016207:Cytokines D004195:Disease Models, Animal D016503:Drug Delivery Systems D007249:Inflammation D039564:Integrin alphaV D008081:Liposomes D008264:Macrophages D008358:Mannose D059630:Mesenchymal Stem Cells D010010:Osteoclasts D010012:Osteogenesis D011422:Propionates D051381:Rats D017208:Rats, Wistar
p-Coumaric acid Mannosylated liposomes Rheumatoid arthritis Osteoclastogenesis Bone erosion