Vitamin D supplement improved testicular function in diabetic rats.
This study was designed to investigate the role that 1,25(OH)2D3 plays against testicular lesion in diabetic rats and try to find its possible mechanism of the steroidogenesis and the spermatogenesis. In diabetic rats, prolonged hyperglycemia evaluated inflammatory cytokines, damaged sperm production function and redox balance, diminished serum testosterone. After treated with 1,25(OH)2D3 at two different doses respectively for 12 months, all the alternations were effectively normalized. 1,25(OH)2D3 showed inhibitory effect on excessive inflammatory biomarkers and adjusted the expression reproductive genes and testicular androgen synthesis. It also upregulated Bcl-2 expression, decreased Bax and COX-2 expression and inhibited active caspase cascade (caspase 8 and caspase 3), which may preserved the testicular cells under diabetic condition. It revealed that vitamin D supplement may protect the cells through suppressing inflammation factors and alleviating cell apoptotic death, as well as upregulating the expression of genes related to reproductive and testosterone synthesis.
['Ding|Chenzhao|C|', 'Wang|Qinzhu|Q|', 'Hao|Yue|Y|', 'Ma|Xiaojun|X|', 'Wu|Lina|L|', 'du|Mengmeng|M|', 'Li|Wen|W|', 'Wu|Yang|Y|', 'Guo|Feng|F|', 'Ma|Siyuan|S|', 'Huang|Fengjuan|F|', 'Qin|Guijun|G|']
D000818:Animals D017209:Apoptosis D001786:Blood Glucose D001835:Body Weight D002117:Calcitriol D053148:Caspase 3 D053181:Caspase 8 D003921:Diabetes Mellitus, Experimental D006943:Hyperglycemia D007248:Infertility, Male D007249:Inflammation D008297:Male D046529:Microscopy, Electron, Transmission D051381:Rats D017207:Rats, Sprague-Dawley D060888:Real-Time Polymerase Chain Reaction D013076:Sperm Count D013094:Spermatozoa D013737:Testis D013739:Testosterone D014807:Vitamin D
Diabetes 1,25(OH)2D3 Inflammation Apoptosis Testicular damage