Twisted intramolecular charge transfer (TICT) based fluorescent probe for lighting up serum albumin with high sensitivity in physiological conditions.
Accurate detection of human serum albumin (HSA) in biological samples is quite meaningful for early disease diagnosis and treatment. Herein, a novel fluorescent probe 1-ethyl-4-[2-[4-(diethylamino)-2-hydroxyphenyl]ethenyl]]-pyridinium salt (DEHP) was developed for HSA determination. The inherent fluorescence of DEHP is essentially negligible at physiological conditions assigned to the well-developed twisted intramolecular charge transfer (TICT) protocol. An intriguing fluorescence light up is triggered as the addition of HSA, on account of the inhibited TICT procedure when DEHP enters the hydrophobic cavity of protein HSA. This combination leads to a turn on fluorescent response for HSA with a detection limit of 4.8 nM. After an overall investigation, it has been proved that the strong binding between DEHP and HSA is specific-site-related. In additional, the probe implies a great potential to assist clinical diagnosis due to the usage in actual serum detection. Cell imaging also shows that the probe is expected to monitor HSA production process at cell level.
['Zhang|Peng|P|', 'Guo|Xinjie|X|', 'Xiao|Yuzhe|Y|', 'Zhang|Qian|Q|', 'Ding|Caifeng|C|']
D002470:Cell Survival D004583:Electrons D005456:Fluorescent Dyes D006367:HeLa Cells D006801:Humans D006863:Hydrogen-Ion Concentration D011725:Pyridines D012709:Serum Albumin D013050:Spectrometry, Fluorescence
TICT Light up HSA Serum detection Cell imaging