The Centriolar Adjunct⁻Appearance and Disassembly in Spermiogenesis and the Potential Impact on Fertility.
During spermiogenesis, the proximal centriole forms a special microtubular structure: the centriolar adjunct. This structure appears at the spermatid stage, which is characterized by a condensed chromatin nucleus. We showed that the centriolar adjunct disappears completely in mature porcine spermatozoa. In humans, the centriolar adjunct remnants are present in a fraction of mature spermatids. For the first time, the structure of the centriolar adjunct in the cell, and its consequent impact on fertility, were examined. Ultrastructural analysis using transmission electron microscopy was performed on near 2000 spermatozoa per person, in two patients with idiopathic male sterility (IMS) and five healthy fertile donors. We measured the average length of the "proximal centriole + centriolar adjunct" complex in sections, where it had parallel orientation in the section plane, and found that it was significantly longer in the spermatozoa of IMS patients than in the spermatozoa of healthy donors. This difference was independent of chromatin condensation deficiency, which was also observed in the spermatozoa of IMS patients. We suggest that zygote arrest may be related to an incompletely disassembled centriolar adjunct in a mature spermatozoon. Therefore, centriolar adjunct length can be potentially used as a complementary criterion for the immaturity of spermatozoa in the diagnostics of IMS patients.
['Garanina|Anastasiia S|AS|', 'Alieva|Irina B|IB|', 'Bragina|Elizaveta E|EE|', 'Blanchard|Emmanuelle|E|', 'Arbeille|Brigitte|B|', 'Guerif|Fabrice|F|', 'Uzbekova|Svetlana|S|', 'Uzbekov|Rustem E|RE|']
D000328:Adult D000818:Animals D002502:Centrioles D002843:Chromatin D005298:Fertility D006801:Humans D007248:Infertility, Male D008297:Male D013087:Spermatids D013091:Spermatogenesis D013552:Swine D014019:Tissue Donors
centriole centriolar adjunct idiopathic male sterility (IMS) centrosome human spermatozoa ultrastructure in vitro fertilization (IVF)