Pectin-encrusted gold nanocomposites containing phytic acid and jacalin: 1,2-dimethylhydrazine-induced colon carcinogenesis in Wistar rats, PI3K/Akt, COX-2, and serum metabolomics as potential targets.
Phytic acid (PA) has momentous chemotherapeutic potential. Due to the chelate formation and rapid elimination, it is not popular in cancer treatment. The present work was inquested to develop a surface-modified nanoformulation of PA which prevents its speedy elimination and maximizes chemotherapeutic action. Chloroauric acid was reduced with pectin to produce pectin-gold nanoparticles (PGNPs). PGNPs were incubated with PA and jacalin for drug loading and surface modifications, respectively, to form PA-loaded jacalin-pectin-gold nanoparticles (PA-J-PGNPs). Formulation(s) were assessed for various pharmaceutical/pharmacological parameters. To validate the efficacy against colon carcinogenesis, formulation(s) were assessed in 1,2-dimethylhydrazine (DMH)-treated Wistar rats. DMH treatment distorted colonic architecture, oxidative, and hemodynamic parameters, which were favorably restored by PA-J-PGNP administration. To further confirm our deliberations, formulation(s) were also examined against DMH-altered metabolic changes and expression of markers pertaining to cellular proliferation, which was reinstated by PA-J-PGNPs. Our findings establish PA formulation(s) as a promising approach for suppression of colon carcinogenesis.
['Arya|Malti|M|', 'Singh|Pooja|P|', 'Tripathi|Chandra B|CB|', 'Parashar|Poonam|P|', 'Singh|Mahendra|M|', 'Kanoujia|Jovita|J|', 'Guleria|Anupam|A|', 'Kaithwas|Gaurav|G|', 'Gupta|Krishna P|KP|', 'Saraf|Shubhini A|SA|http://orcid.org/0000-0002-4180-0931']
D019813:1,2-Dimethylhydrazine D000818:Animals D002712:Chlorides D003110:Colonic Neoplasms D051546:Cyclooxygenase 2 D004339:Drug Compounding D015972:Gene Expression Regulation, Neoplastic D017612:Gold Compounds D055432:Metabolomics D053768:Metal Nanoparticles D019869:Phosphatidylinositol 3-Kinases D010833:Phytic Acid D037121:Plant Lectins D051057:Proto-Oncogene Proteins c-akt D051381:Rats D017208:Rats, Wistar D023041:Xenograft Model Antitumor Assays
Surface modification 1H NMR spectroscopy Hemodynamic changes IP6 RT-PCR Western blotting