Trans-synaptic and retrograde axonal spread of Lewy pathology following pre-formed fibril injection in an in vivo A53T alpha-synuclein mouse model of synucleinopathy.
It is necessary to develop an understanding of the specific mechanisms involved in alpha-synuclein aggregation and propagation to develop disease modifying therapies for age-related synucleinopathies, including Parkinson's disease and Dementia with Lewy Bodies. To adequately address this question, we developed a new transgenic mouse model of synucleinopathy that expresses human A53T SynGFP under control of the mouse prion protein promoter. Our characterization of this mouse line demonstrates that it exhibits several distinct advantages over other, currently available, mouse models. This new model allows rigorous study of the initial location of Lewy pathology formation and propagation in the living brain, and strongly suggests that aggregation begins in axonal structures with retrograde propagation to the cell body. This model also shows expeditious development of alpha-synuclein pathology following induction with small, in vitro-generated alpha-synuclein pre-formed fibrils (PFFs), as well as accelerated cell death of inclusion-bearing cells. Using this model, we found that aggregated alpha-synuclein somatic inclusions developed first in neurons, but later showed a second wave of inclusion formation in astrocytes. Interestingly, astrocytes appear to survive much longer after inclusion formation than their neuronal counterparts. This model also allowed careful study of peripheral-to-central spread of Lewy pathology after PFF injection into the hind limb musculature. Our results clearly show evidence of progressive, retrograde trans-synaptic spread of Lewy pathology through known neuroanatomically connected pathways in the motor system. As such, we have developed a promising tool to understand the biology of neurodegeneration associated with alpha-synuclein aggregation and to discover new treatments capable of altering the neurodegenerative disease course of synucleinopathies.
['Schaser|Allison J|AJ|', 'Stackhouse|Teresa L|TL|', 'Weston|Leah J|LJ|', 'Kerstein|Patrick C|PC|', 'Osterberg|Valerie R|VR|', 'López|Claudia S|CS|', 'Dickson|Dennis W|DW|', 'Luk|Kelvin C|KC|', 'Meshul|Charles K|CK|', 'Woltjer|Randall L|RL|', 'Unni|Vivek K|VK|0000-0001-8833-6542']
D000818:Animals D001253:Astrocytes D001369:Axons D001921:Brain D004195:Disease Models, Animal D005260:Female D006801:Humans D016631:Lewy Bodies D008297:Male D051379:Mice D008822:Mice, Transgenic D009474:Neurons D021381:Protein Transport D000080874:Synucleinopathies D051844:alpha-Synuclein
Alpha-synuclein Synucleinopathies Dementia with Lewy bodies Lewy body Trans-synaptic spread Neurodegeneration Parkinson’s disease