Revealing membrane permeability of polymersomes through fluorescence enhancement.
In this work, permeability of polymersomes was quantified by fluorescence enhancement. The polymersomes were prepared from poly(ethylene glycol)-b-poly(ε-caprolactone) (PEG-PCL), and an acid-sensitive hydrophobic dye borondipyrromethene (BODIPY) was encapsulated into the membrane of polymersomes. H+ from trifluoroacetic acid (TFA) would diffuse into the membrane of polymersomes to enhance the fluorescence of BODIPY. Intensity of the enhanced fluorescence could be used for quantifying the H+ permeability of polymersomes. In addition, poly(ε-caprolactone) (PCL) was used to control the permeability ability of the membrane. Such quantitative information on the molecular diffusion of polymersomes is of fundamental importance to many of their potential applications, e.g., nanoreactors and drug delivery.
['Quan|Li|L|', 'Ding|Hongyan|H|', 'Pan|Changjiang|C|', 'Wei|Yanchun|Y|', 'Xie|Zhigang|Z|']
D001896:Boron Compounds D002463:Cell Membrane Permeability D004337:Drug Carriers D016503:Drug Delivery Systems D005026:Ethylene Glycols D005453:Fluorescence D057927:Hydrophobic and Hydrophilic Interactions D008567:Membranes, Artificial D046529:Microscopy, Electron, Transmission D010539:Permeability D011091:Polyesters
Permeability Polymersomes Fluorescence Bodipy