Polyomavirus BK and JC in individuals with chronic kidney failure, kidney transplantation, and healthy controls.
BACKGROUND
New clinical approaches to diagnose and monitor individuals with systemic diseases have been employed through the use of oral fluids. Polyomavirus BK (BKPyV) and JC (JCPyV) infect asymptomatically around 80% of general population worldwide remaining latent in the body. In case of immunosuppression, a replication can occur, leading to diseases.


OBJECTIVE
The aim of this study was to detect and quantify BKPyV and JCPyV in oral fluids of individuals with chronic kidney failure (CKF), kidney transplantation (KT) and controls compared with their detection in blood and urine, traditionally used for this test.


STUDY DESIGN
Forty six subjects were included and distributed into 3 groups: 14 with CKF (Group 1), 12 with KT (Group 2) and 20 healthy individuals (Group 3). In a total, 315 samples were collected and analyzed through RT-PCR, being 151 of gingival crevicular fluid, 46 of saliva, 46 of mouthwash, 43 of blood and 29 of urine.


RESULTS
All subjects from group 1 were positive for BKPyV in at least one collected samples and 14% were positive for JCPyV. In Group 2, 91.7% were positive for BKPyV and 51.7% for JCPyV. Among subjects of Group 3, 80% were positive for BKPyV and 45% for JCPyV.


CONCLUSIONS
Oral fluids exhibited high prevalence of BKPyV and JCPyV and were equally efficient compared to urine and blood. The use of oral fluids to detect these polyomaviruses enhances positivity in screening, even in cases of absence of viremia and especially in individuals who are not able to urinate.
['Castro|Talita|T|', 'Fink|Maria Cristina Domingues|MCD|', 'Figueiredo|Marilia|M|', 'Braz-Silva|Paulo Henrique|PH|', 'Pannuti|Cláudio Mendes|CM|', 'Ortega|Karem Lopez|KL|', 'Gallottini|Marina|M|']
D000328:Adult D000368:Aged D001739:BK Virus D001769:Blood D005260:Female D006801:Humans D007577:JC Virus D007676:Kidney Failure, Chronic D016030:Kidney Transplantation D008297:Male D008875:Middle Aged D009055:Mouth D027601:Polyomavirus Infections D060888:Real-Time Polymerase Chain Reaction D012189:Retrospective Studies D014412:Tumor Virus Infections D014556:Urine D019562:Viral Load
Chronic kidney failure Kidney transplantation Gingival crevicular fluid Saliva BK virus JC virus