Modulation of VEGF Expression and Oxidative Stress Response by Iodine Deficiency in Irradiated Cancerous and Non-Cancerous Breast Cells.
Breast cancer remains a major concern and its physiopathology is influenced by iodine deficiency (ID) and radiation exposure. Since radiation and ID can separately induce oxidative stress (OS) and microvascular responses in breast, their combination could additively increase these responses. Therefore, ID was induced in MCF7 and MCF12A breast cell lines by medium change. Cells were then X-irradiated with doses of 0.05, 0.1, or 3 Gy. In MCF12A cells, both ID and radiation (0.1 and 3 Gy) increased OS and vascular endothelial growth factor (VEGF) expression, with an additive effect when the highest dose was combined with ID. However, in MCF7 cells no additive effect was observed. VEGF mRNA up-regulation was reactive oxygen species (ROS)-dependent, involving radiation-induced mitochondrial ROS. Results on total VEGF mRNA hold true for the pro-angiogenic isoform VEGF165 mRNA, but the treatments did not modulate the anti-angiogenic isoform VEGF165b. Radiation-induced antioxidant response was differentially regulated upon ID in both cell lines. Thus, radiation response is modulated according to iodine status and cell type and can lead to additive effects on ROS and VEGF. As these are often involved in cancer initiation and progression, we believe that iodine status should be taken into account in radiation prevention policies.
['Vanderstraeten|Jessica|J|', 'Baselet|Bjorn|B|', 'Buset|Jasmine|J|', 'Ben Said|Naziha|N|', 'de Ville de Goyet|Christine|C|', 'Many|Marie-Christine|MC|', 'Gérard|Anne-Catherine|AC|', 'Derradji|Hanane|H|']
D000975:Antioxidants D001940:Breast D001943:Breast Neoplasms D045744:Cell Line, Tumor D004305:Dose-Response Relationship, Drug D005260:Female D006801:Humans D007455:Iodine D061986:MCF-7 Cells D009389:Neovascularization, Pathologic D018384:Oxidative Stress D012333:RNA, Messenger D017382:Reactive Oxygen Species D015854:Up-Regulation D042461:Vascular Endothelial Growth Factor A
VEGF oxidative stress iodine deficiency breast radiation MCF7 MCF12A ROS