Linc-9432 is a novel pterygium lincRNA which regulates differentiation of fibroblasts.
Long intergenic noncoding RNAs (lincRNAs) are not fully characterized in disease, although many are involved in controlling differentiation. We discovered and sequenced a novel 4.4 kb human lincRNA called linc-9432 in pterygium, an ocular disease characterized by a wedge lesion. This transcript inhibited differentiation-induced cell death, promoted expression of stem cell markers, and decreased expression of epithelial and mesenchymal differentiation markers. This lincRNA regulated 30 differentiation-related genes in transcriptome analysis and 17/30 gene products were known to be directly associated in a network. When the lincRNA was silenced with pooled siRNA, the levels of these transcripts decreased in accordance with their predicted binding affinity for the lincRNA. TBC1D8B had the strongest affinity, interacting in-vitro at positions 269-292 within TBC1D8B.
['Lan|Wanwen|W|', 'Hou|Aihua|A|', 'Lakshminarayanan|Rajamani|R|', 'Lim|Yoon-Pin|YP|', 'Tong|Louis|L|']
D000943:Antigens, Differentiation D002454:Cell Differentiation D002478:Cells, Cultured D005260:Female D005347:Fibroblasts D020869:Gene Expression Profiling D005786:Gene Expression Regulation D006801:Humans D008297:Male D011625:Pterygium D062085:RNA, Long Noncoding
pterygium noncoding RNA human cell proliferation conjunctiva ocular surface disease pathology post-transcriptional control