Airway microbiota in patients with paediatric cystic fibrosis: Relationship with clinical status.
INTRODUCTION
New massive sequencing techniques make it possible to determine the composition of airway microbiota in patients with cystic fibrosis (CF). However, the relationship between the composition of lung microbiome and the clinical status of paediatric patients is still not fully understood.


MATERIAL AND METHODS
A cross-sectional observational study was conducted on induced sputum samples from children with CF and known mutation in the CFTR gene. The bacterial sequences of the 16SrRNA gene were analyzed and their association with various clinical variables studied.


RESULTS
Analysis of the 13 samples obtained showed a core microbiome made up of Staphylococcus spp., Streptococcus spp., Rothia spp., Gemella spp. and Granulicatella spp., with a small number of Pseudomonas spp. The cluster of patients with less biodiversity were found to exhibit a greater number of sequences of Staphylococcus spp., mainly Staphylococcus aureus (p 0.009) and a greater degree of lung damage.


CONCLUSION
An airway microbiome with greater biodiversity may be an indicator of less pronounced disease progression, in which case new therapeutic interventions that prevent reduction in non-pathogenic species of the airway microbiota should be studied.
['Sánchez-Bautista|Antonia|A|', 'Rodríguez-Díaz|Juan Carlos|JC|', 'Garcia-Heredia|Inmaculada|I|', 'Luna-Paredes|Carmen|C|', 'Alcalá-Minagorre|Pedro J|PJ|']
D000293:Adolescent D002648:Child D003430:Cross-Sectional Studies D003550:Cystic Fibrosis D005260:Female D006801:Humans D008297:Male D064307:Microbiota D012137:Respiratory System D013183:Sputum
Cystic fibrosis Lung microbiome Paediatric patients Edad pediátrica Enfermedad pulmonar Fibrosis quística Lung disease Pulmón