Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.
Autophagy is a highly conserved cellular mechanism of "self digestion," ensuring cellular homeostasis, and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy.LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.
['Berezowska|Sabina|S|', 'Galván|José A|JA|']
D001343:Autophagy D015415:Biomarkers D006801:Humans D007150:Immunohistochemistry D008869:Microtubule-Associated Proteins D009369:Neoplasms D016612:Paraffin Embedding D000071456:Sequestosome-1 Protein
Autophagy LC3 p62/SQSTM1 Sequestosome 1 Immunohistochemistry