Synthesis of Chitosan Beads Incorporating Graphene Oxide/Titanium Dioxide Nanoparticles for In Vivo Studies.
Scaffold development for cell regeneration has increased in recent years due to the high demand for more efficient and biocompatible materials. Nanomaterials have become a critical alternative for mechanical, thermal, and antimicrobial property reinforcement in several biopolymers. In this work, four different chitosan (CS) bead formulations crosslinked with glutaraldehyde (GLA), including titanium dioxide nanoparticles (TiO2), and graphene oxide (GO) nanosheets, were prepared with potential biomedical applications in mind. The characterization of by FTIR spectroscopy, X-ray photoelectron spectroscopy (XRD), thermogravimetric analysis (TGA), energy-dispersive spectroscopy (EDS) and scanning electron microscopy (SEM), demonstrated an efficient preparation of nanocomposites, with nanoparticles well-dispersed in the polymer matrix. In vivo, subdermal implantation of the beads in Wistar rat's tissue for 90 days showed a proper and complete healing process without any allergenic response to any of the formulations. Masson's trichrome staining of the histological implanted tissues demonstrated the presence of a group of macrophage/histiocyte compatible cells, which indicates a high degree of biocompatibility of the beads. The materials were very stable under body conditions as the morphometry studies showed, but with low resorption percentages. These high stability beads could be used as biocompatible, resistant materials for long-term applications. The results presented in this study show the enormous potential of these chitosan nanocomposites in cell regeneration and biomedical applications.
['Grande Tovar|Carlos David|CD|0000-0002-6243-4571', 'Castro|Jorge Iván|JI|', 'Valencia|Carlos Humberto|CH|', 'Zapata|Paula A|PA|', 'Solano|Moisés A|MA|', 'Florez López|Edwin|E|', 'Chaur|Manuel N|MN|', 'Valencia Zapata|Mayra Eliana|ME|0000-0001-9001-2045', 'Mina Hernandez|José Herminsul|JH|0000-0003-3767-4431']
D000818:Animals D001672:Biocompatible Materials D002470:Cell Survival D048271:Chitosan D006108:Graphite D006644:Histiocytes D008297:Male D053761:Nanocomposites D053758:Nanoparticles D051381:Rats D017208:Rats, Wistar D012867:Skin D023822:Tissue Engineering D054457:Tissue Scaffolds D014025:Titanium
chitosan beads titanium dioxide nanoparticles nanocomposites graphene-oxide tissue engineering