The role of fibroblast activation protein in progression and development of osteosarcoma cells.
To investigate the expression levels of fibroblast activation protein (FAP) in human osteosarcoma tissues and its possible correlations with clinical pathological characteristics of patients with osteosarcoma, and to explore the potential effects of FAP on progression and development of osteosarcoma. Immunohistochemistry (IHC) assay was initially performed to detect the expression levels of FAP in 66 tumor tissues and adjacent non-tumor tissues. Patients were sequentially divided into two groups based on different expression levels of FAP. The correlations between the expression levels of FAP and the clinical pathological characteristics were investigated, and the role of FAP in proliferation, migration, and invasion of osteosarcoma cells was assessed via colony formation, MTT, wound healing, and transwell assays, respectively. The possible effects of FAP on tumor growth and metastasis were evaluated in vivo. We further attempted to reveal the underlying mechanism of FAP involved in tumor growth through bioinformatics and IHC assays. High expression levels of FAP were noted in human osteosarcoma tissues. It also was unveiled that FAP was significantly associated with the tumor size (P = 0.005*) and clinical stage (P = 0.017*). Our data further confirmed that knockdown of FAP remarkably blocked proliferation, migration, and invasion of osteosarcoma cells in vitro, and suppressed tumor growth and metastasis in mice via AKT signaling pathway. The possible role of FAP in progression and development of osteosarcoma could be figured out. Our data may be helpful to develop a novel therapeutic target for the treatment of osteosarcoma.
['Zhang|Liang|L|', 'Yang|Li|L|', 'Xia|Zi-Wei|ZW|', 'Yang|Shi-Chang|SC|', 'Li|Wen-Hui|WH|', 'Liu|Bin|B|', 'Yu|Zi-Qi|ZQ|', 'Gong|Peng-Fei|PF|', 'Yang|Ya-Lin|YL|', 'Sun|Wei-Zong|WZ|', 'Mo|Jing|J|', 'Li|Gui-Shi|GS|', 'Wang|Tian-Yi|TY|', 'Wang|Kai|K|http://orcid.org/0000-0002-3691-1485']
D000818:Animals D001859:Bone Neoplasms D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D018450:Disease Progression D010450:Endopeptidases D005260:Female D018093:Gelatinases D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008297:Male D008565:Membrane Proteins D051379:Mice D009367:Neoplasm Staging D009368:Neoplasm Transplantation D012516:Osteosarcoma D012697:Serine Endopeptidases D015398:Signal Transduction D015854:Up-Regulation
Fibroblast activation protein Osteosarcoma Proliferation Migration Invasion AKT signaling pathway