Purification, structural characterization and bioactivity evaluation of a novel proteoglycan produced by Corbicula fluminea.
A novel proteoglycan, named CFPS-11, was isolated from Corbicula fluminea, which is a food source of freshwater bivalve mollusk. CFPS-11 had an average molecular weight of 807.7kDa and consisted of d-glucose and d-glucosamine in a molar ratio of 12.2:1.0. The protein moiety (∼5%) of CFPS-11 was covalently bonded to the polysaccharide chain in O-linkage type through both serine and thereonine residues. The polysaccharide chain of CFPS-11 was composed of (1→4)-α-d-glucopyranosyl and (1→3,6)-α-d-glucopyranosyl residues, which branched at O-6. The branch chain consisted of (1→)-α-d-glucopyranosyl and (1→)-α-d-N-acetylglucosamine residues. CFPS-11 exhibited significant antioxidant activity in a dose-dependent manner and remarkable inhibition activities against α-amylase and α-glucosidase by in vitro assays. These findings indicated that the CFPS-11 from C. fluminea has the potential for development as a health food ingredient.
['Yan|Jing-Kun|JK|', 'Wang|Yao-Yao|YY|', 'Qiu|Wen-Yi|WY|', 'Wu|Li-Xia|LX|', 'Ding|Zhi-Chao|ZC|', 'Cai|Wu-Dan|WD|']
D000818:Animals D049874:Corbicula D005618:Fresh Water D008970:Molecular Weight D011509:Proteoglycans
Purification Structural characterization Proteoglycan Corbicula fluminea Antioxidant activity