Associations of TNF-α -238 A/G and IL-10 -1082 G/A Genetic Polymorphisms With the Risk of NONFH in the Chinese Population.
The study aims to explore the roles of common polymorphisms of Tumor Necrosis Factor-α (TNF-α) (-238 A/G and -308 A/G) and IL-10 (-819 T/C and -1082 G/A) genes in the risk of non-traumatic osteonecrosis of the femoral head (NONFH). One hundred and forty-seven NONFH patients and 135 healthy individuals were selected as the case and control groups. qRT-PCR and Western blotting techniques detected mRNA as well as protein expressions of TNF-α and IL-10 of each genotype in both the case and control groups. The GA genotype and the A allele of TNF-α -238 A/G were higher in the case group than in the control group. Compared with the control group, AA, GA, and AG + AA genotypes as well as the A allele of IL-10-1082 G/A were all lower in the case group. In the case groups increased levels of TNF-α as well as decreased levels of IL-10 expression when compared with the control group. TNF-α expression of TNF-α-238 GA genotype was significantly higher than that in patients with GG genotype, while the IL-10 expression of GA and AA genotypes of IL-10-1082 was significantly lower than in that of patients with the GG genotype. TNF-α protein expression in the GA genotype was significantly higher than in the GG genotype. In relation to TNF-α -238, TNF-α protein expression of GA and AA genotypes had significantly reduced more so than the GG genotype in IL-10-1082. TNF-α-238 A/G and IL-10-1082 G/A may be involved as risk factors of NONFH. J. Cell. Biochem. 118: 4872-4880, 2017. © 2017 Wiley Periodicals, Inc.
['Wei|Biao-Fang|BF|', 'Feng|Zhi|Z|', 'Wei|Wei|W|0000-0001-9947-0341', 'Chen|Xiao|X|']
D044466:Asians D002648:Child D002675:Child, Preschool D002681:China D005260:Female D005271:Femur Head Necrosis D005838:Genotype D006801:Humans D016753:Interleukin-10 D008297:Male D011110:Polymorphism, Genetic D014409:Tumor Necrosis Factor-alpha
GENOTYPE BLOOD LIPID LEVEL GENE POLYMORPHISMS INTERLEUKIN-10 NON-TRAUMATIC OSTEONECROSIS OF FEMORAL HEAD TUMOR NECROSIS FACTOR α