Risk estimation of the low-dose fast neutrons on the molecular structure of the lipids of peripheral blood mononuclear cells.
Our immune system can be exposed to neutral ionizing radiation. This work proposed to investigate the impacts of low-dose fast neutrons on the molecular structure of the lipids of peripheral blood mononuclear cells (PBMCs) as the primary immune cells. The effects have been followed at the time of 0, 2, 4, and 8 days after neutron exposure. Sixty-four female Wistar rats were used in this work. Thirty-two of them were irradiated using a low-dose fast neutron (241Am-Be, 0.2 mGy/h). The other thirty-two were utilized as a control group. The changes in their lipids molecular structure were investigated using Fourier transform infrared (FTIR) spectroscopy. Besides, lipids were extracted, and the total phospholipids contents were estimated. At the time of 2 and 4 days after irradiation, the results showed significant changes in the molecular structure of lipids in those irradiated samples compare with their control samples, and multivariate analysis succeeded in differentiating between control and irradiated rats. In contrast, no alterations between control and irradiated were detected at the time of 0 and 8 days after. The low-dose fast neutron could induce free radicals, which indirectly affect the molecular structure of the lipids and could damage the phospholipids molecules, and the damage could be repaired.
['Saeed|Abdu|A|', 'Abolaban|Fouad|F|']
D000818:Animals D005214:Fast Neutrons D005260:Female D007963:Leukocytes, Mononuclear D015394:Molecular Structure D010743:Phospholipids D025341:Principal Component Analysis D011839:Radiation, Ionizing D051381:Rats D017208:Rats, Wistar D012307:Risk Factors D017550:Spectroscopy, Fourier Transform Infrared
Low-dose fast neutrons The molecular structure of the lipids PBMCs FTIR spectroscopy