Functional analysis of miR-181a and Fas involved in hepatitis B virus-related hepatocellular carcinoma pathogenesis.
The hepatitis B virus (HBV) is responsible for most of hepatocellular carcinoma (HCC). However, whether HBV plays an important role during hepatocarcinogenesis through effecting miRNAs remains unknown. Here, we reported that HBV up-regulated microRNA-181a (miR-181a) by enhancing its promoter activity. Simultaneously, we found that miR-181a inhibited apoptosis in vitro and promoted tumor cell growth in vivo. TNF receptor superfamily member 6 (Fas) was further identified as a target of miR-181a. We also found that Fas could reverse the apoptosis-inhibition effect induced by miR-181a. Moreover, HBV could inhibit cell apoptosis by down-regulating Fas expression, which could be reversed by miR-181a inhibitor. Our data demonstrated that HBV suppressed apoptosis of hepatoma cells by up-regulating miR-181a expression and down-regulating Fas expression, which may provide a new understanding of the mechanism in HBV-related HCC pathogenesis.
['Zou|Chengcheng|C|', 'Chen|Juan|J|', 'Chen|Ke|K|', 'Wang|Sen|S|', 'Cao|Yiyi|Y|', 'Zhang|Jinnan|J|', 'Sheng|Yanrui|Y|', 'Huang|Ailong|A|', 'Tang|Hua|H|']
D000818:Animals D017209:Apoptosis D006528:Carcinoma, Hepatocellular D053181:Caspase 8 D045744:Cell Line, Tumor D049109:Cell Proliferation D002471:Cell Transformation, Neoplastic D004279:DNA, Viral D015536:Down-Regulation D056945:Hep G2 Cells D006509:Hepatitis B D006515:Hepatitis B virus D006801:Humans D008113:Liver Neoplasms D051379:Mice D008819:Mice, Nude D035683:MicroRNAs D011401:Promoter Regions, Genetic D015533:Transcriptional Activation D047368:Tumor Burden D015854:Up-Regulation D019014:fas Receptor
miR-181a Fas HBV HCC Apoptosis