MicroRNA-195a-5p inhibits mouse medullary thymic epithelial cells proliferation by directly targeting Smad7.
MiR-195 has been implicated in inhibiting cell proliferation in different types of tumors. Whether it contributes to the process of thymic epithelial cells (TECs) proliferation remains unclear. In this study, we found that miR-195a-5p was highly up-regulated in the TECs isolated from the aging mice. Further experiments showed that miR-195a-5p mimic transfection inhibited the proliferation of mouse medullary thymic epithelial cell line 1 (MTEC1), whereas the transfection of miR-195a-5p inhibitor in MTEC1 had the opposite effect. In addition, miR-195a-5p had no obvious effect on MTEC1 apoptosis. Furthermore, Smad7, a negative regulator of transforming growth factor β pathway, was confirmed as a direct target of miR-195a-5p by luciferase assays. Taken together, our results indicate that miR-195a-5p inhibits MTEC1 proliferation, at least in part, via down-regulation of Smad7.
['Guo|Dongguang|D|', 'Ye|Yaqiong|Y|', 'Qi|Junjie|J|', 'Xu|Lifeng|L|', 'Zhang|Lihua|L|', 'Tan|Xiaotong|X|', 'Tan|Zhigang|Z|', 'Yu|Xiaofang|X|', 'Zhang|Yuan|Y|', 'Ma|Yongjiang|Y|', 'Li|Yugu|Y|']
D000818:Animals D017209:Apoptosis D049109:Cell Proliferation D004847:Epithelial Cells D051379:Mice D008807:Mice, Inbred BALB C D035683:MicroRNAs D051906:Smad7 Protein D013950:Thymus Gland
miR-195a-5p cell proliferation, Smad7 mouse thymic epithelial cell