Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor.
Granulocyte-monocyte progenitors (GMPs) have been previously defined for their potential to generate various myeloid progenies such as neutrophils and monocytes. Although studies have proposed lineage heterogeneity within GMPs, it is unclear if committed progenitors already exist among these progenitors and how they may behave differently during inflammation. By combining single-cell transcriptomic and proteomic analyses, we identified the early committed progenitor within the GMPs responsible for the strict production of neutrophils, which we designate as proNeu1. Our dissection of the GMP hierarchy led us to further identify a previously unknown intermediate proNeu2 population. Similar populations could be detected in human samples. proNeu1s, but not proNeu2s, selectively expanded during the early phase of sepsis at the expense of monocytes. Collectively, our findings help shape the neutrophil maturation trajectory roadmap and challenge the current definition of GMPs.
['Kwok|Immanuel|I|', 'Becht|Etienne|E|', 'Xia|Yu|Y|', 'Ng|Melissa|M|', 'Teh|Ye Chean|YC|', 'Tan|Leonard|L|', 'Evrard|Maximilien|M|', 'Li|Jackson L Y|JLY|', 'Tran|Hoa T N|HTN|', 'Tan|Yingrou|Y|', 'Liu|Dehua|D|', 'Mishra|Archita|A|', 'Liong|Ka Hang|KH|', 'Leong|Keith|K|', 'Zhang|Yuning|Y|', 'Olsson|Andre|A|', 'Mantri|Chinmay Kumar|CK|', 'Shyamsunder|Pavithra|P|', 'Liu|Zhaoyuan|Z|', 'Piot|Cecile|C|', 'Dutertre|Charles-Antoine|CA|', 'Cheng|Hui|H|', 'Bari|Sudipto|S|', 'Ang|Nicholas|N|', 'Biswas|Subhra K|SK|', 'Koeffler|H Philip|HP|', 'Tey|Hong Liang|HL|', 'Larbi|Anis|A|', 'Su|I-Hsin|IH|', 'Lee|Bernett|B|', 'St John|Ashley|A|', 'Chan|Jerry K Y|JKY|', 'Hwang|William Y K|WYK|', 'Chen|Jinmiao|J|', 'Salomonis|Nathan|N|', 'Chong|Shu Zhen|SZ|', 'Grimes|H Leighton|HL|', 'Liu|Bing|B|', 'Hidalgo|Andrés|A|', 'Newell|Evan W|EW|', 'Cheng|Tao|T|', 'Ginhoux|Florent|F|', 'Ng|Lai Guan|LG|']
D000818:Animals D042381:Granulocyte Precursor Cells D006801:Humans D051379:Mice D008810:Mice, Inbred C57BL D018345:Mice, Knockout D009000:Monocytes D038042:Myelopoiesis D009504:Neutrophils D059010:Single-Cell Analysis
granulocyte-monocyte progenitor emergency granulopoiesis granulopoiesis hematopoiesis myeloid cells myelopoiesis neutrophil development pro-neutrophil