An orally administered magnoloside A ameliorates functional dyspepsia by modulating brain-gut peptides and gut microbiota.
AIMS
Functional dyspepsia (FD) is very common worldwide with a high prevalence of 10%-30%, and it becomes a heavy burden to patients because of its hard to be cured. In our previous study, phenylethanoid glycosides were found to exist in Houpo, a traditional Chinese medicine commonly used for the treatment of abdominal distention, pain and dyspepsia. In the present study, the effect of magnoloside A (MA), a main phenylethanoid glycoside in Houpo, on FD was firstly evaluated and its potential mechanism was concluded.


MATERIALS AND METHODS
MA was orally administered consequently for 3 weeks, and its effect on a FD rat model established through transient neonatal gastric irritation and mature alternate-day fasting was tested. Levels of brain-gut peptides and inflammatory factors in blood or tissues were determined by ELISA methods. Meanwhile, the gut microbiota was analyzed by 16S rRNA gene sequencing and short chain fat acids were determined by GC/MS.


KEY FINDINGS
MA exhibited anti-FD activities by fastening the delayed gut emptying rate of FD rat and increasing the levels of gastrin, motilin, and calcitonin gene related protein; and decreasing the levels of 5-hydroxytryptamine, nitric oxide synthase, and vasoactive intestinal peptide. On the other hand, MA can modulate the composition of gut microbiota, resulting in the variation of the short chain fat acids.


SIGNIFICANCE
MA ameliorated FD rats by modulating of the secretion of related brain-gut peptides and altering the composition of intestinal microbiota.
['Xue|Zhenzhen|Z|', 'Wu|Changxun|C|', 'Wei|Junying|J|', 'Xian|Minghua|M|', 'Wang|Tingting|T|', 'Yang|Bin|B|', 'Chen|Min|M|']
D000284:Administration, Oral D000818:Animals D000831:Animals, Newborn D001921:Brain D004415:Dyspepsia D005746:Gastric Emptying D000069196:Gastrointestinal Microbiome D041981:Gastrointestinal Tract D006027:Glycosides D031566:Magnolia D008297:Male D010446:Peptide Fragments D010626:Phenylethyl Alcohol D051381:Rats D017207:Rats, Sprague-Dawley
Magnoloside A Functional dyspepsia Brain-gut peptides Gut microbiota Houpo Short chain fat acids