Alteration of sFAS and sFAS ligand expression during canine visceral leishmaniosis.
Visceral leishmaniosis (VL) is caused by intracellular parasites of the genus Leishmania that affect humans and several animal species. Dogs are one of the main urban reservoirs of Leishmania infantum and play a central role in the transmission cycle to humans via sandflies. CD3+ cells apoptosis is involved in the immune response in VL. Dysregulation of apoptosis has been implicated in various disease states. An important regulator of apoptosis is the FAS-FAS-associated death domain protein (cluster of differentiation 95 - CD95) and FASL-FAS ligand protein (cluster of differentiation 178 - CD178) system involved in the down-regulation of immune reactions and in T cell-mediated cytotoxicity. FAS is a member of the tumor necrosis factor (TNF) receptor super family, which can be expressed in transmembrane or soluble forms. The soluble levels of FAS (sFAS), FASL (sFASL) and active Caspase-3, this last related to apoptotic cascade, were investigated in the spleen of 19 symptomatic dogs presenting moderate VL and 6 healthy dogs, determined by ELISA assay. The splenic parasite load was determined by real-time PCR monitoring of amplification of the intergenic internal transcribed spacer (ITS1) gene of parasite rRNA. sFAS levels were lower (p<0.05). sFASL and active Caspase-3 levels were higher (p<0.05) in dogs with VL compared with controls. Negative correlation was observed between parasite burden and sFASL levels. The increase in sFASL could be related to the mechanism involved in the elimination of the parasite.
['Perosso|Juliana|J|', 'Silva|Kathlenn Liezbeth Oliveira|KL|', 'Ferreira|Stefáni Íris de Souza|SÍ|', 'Avanço|Saulo Vinícius|SV|', 'dos Santos|Paulo Sérgio Patto|PS|', 'Eugênio|Flávia de Rezende|Fde R|', 'de Almeida|Breno Fernando Martins|BF|', 'de Lima|Valéria Marçal Felix|VM|']
D000818:Animals D017209:Apoptosis D016923:Cell Death D004283:Dog Diseases D004285:Dogs D015536:Down-Regulation D053222:Fas Ligand Protein D005786:Gene Expression Regulation D006801:Humans D018314:Leishmania infantum D007898:Leishmaniasis, Visceral D013154:Spleen D053221:TNF-Related Apoptosis-Inducing Ligand D019014:fas Receptor
Leishmaniosis Leishmania infantum Cell death FAS ligand protein FAS-associated death domain protein TRAIL (TNF-related apoptosis-inducing ligand)