Salvianolic acid B inhibits IL-1β-induced inflammatory cytokine production in human osteoarthritis chondrocytes and has a protective effect in a mouse osteoarthritis model.
Osteoarthritis (OA) is a chronic progressive disease that has complicated mechanisms that involve inflammation and cartilage degradation. In this study, we investigated the anti-inflammatory action of Salvianolic acid B (Sal B) in both human OA chondrocytes and a mouse OA model that was induced by destabilization of the medial meniscus. In vitro, chondrocytes were pretreated with Sal B (0, 25, 50, 100μM) for 2h, then incubated with IL-1β (10ng/mL) for 24h. NO production was determined by Griess method and PGE2 was assessed by ELISA. The expression of INOS, COX-2, MMP-13, ADAMTS-5 and NF-κB-related signaling molecules were tested by Western blotting. Immunofluorescence staining was used to detect P65 nuclear translocation. In vivo, the mouse OA model received intraperitoneal-injection of either Sal B (25mg/kg) or saline every other day. Hematoxylin and Eosin, as well as Safranin-O-Fast green staining, were utilized to evaluate the severity of cartilage lesions up to 8weeks following the surgery. Sal B inhibited the over-production of NO and PGE2, while the elevated expression of INOS, COX-2, MMP-13 and ADAMTS-5 were reversed by Sal B in IL-1β-induced chondrocytes. In addition, IL-1β significantly induced phosphorylation of NF-κB signaling, and this phosphorylation response was blocked by Sal B. Immunofluorescence staining demonstrated that Sal B could suppress IL-1β-induced p65 nuclear translocation. In vivo, the cartilage in Sal B-treated mice exhibited less cartilage degradation and lower OARSI scores. Taken together, Sal B possesses great potential value as a therapeutic agent for OA treatment.
['Lou|Yiting|Y|', 'Wang|Chenggui|C|', 'Zheng|Wenhao|W|', 'Tang|Qian|Q|', 'Chen|Yu|Y|', 'Zhang|Xiaolei|X|', 'Guo|Xiaoshan|X|', 'Wang|Jianshun|J|']
D000818:Animals D000893:Anti-Inflammatory Agents D001572:Benzofurans D002356:Cartilage D019902:Chondrocytes D016207:Cytokines D004195:Disease Models, Animal D006801:Humans D007249:Inflammation D053583:Interleukin-1beta D008297:Male D053509:Matrix Metalloproteinase 13 D008592:Menisci, Tibial D051379:Mice D008810:Mice, Inbred C57BL D016328:NF-kappa B D009569:Nitric Oxide D010003:Osteoarthritis D010766:Phosphorylation D015398:Signal Transduction
Salvianolic acid B Osteoarthritis Chondrocyte NF-κB Interleukin-1β