Streptomyces venetus sp. nov., an actinomycete with a blue aerial mycelium.
A Gram-positive bacterium, designated CMU-AB225T, was isolated from rhizosphere soil of an oil palm (Elaeis guineensis). The strain exhibited a blue aerial spore mass and a light cream to moderate yellow substrate mycelium and formed chains of spiny spores. Whole-cell hydrolysates consisted of ll-diaminopimelic acid, glucose, ribose, mannose and galactose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The polar lipids profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside, four unidentified lipids, two unidentified aminolipids and an unidentified glycolipid. The major cellular fatty acids (>10 %) were iso-C16 : 0, C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The G+C content of genomic DNA was 69.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMU-AB225T was a member of the genus Streptomyces and formed a distinct phyletic line which was most closely related to Streptomyces koyangensis JCM 14915T, Streptomyces misionensis JCM 4497T and Streptomyces aurantiogriseus JCM 4346T. Multilocus sequence analysis (MLSA) using five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed that the MLSA distances of strain CMU-AB225T to phylogenetically related species were greater than the 0.007 threshold. Moreover, the low values of DNA-DNA relatedness and phenotypic differences, especially a blue aerial mycelium, enabled strain CMU-AB225T to be distinguished from its closely related species. It is thus proposed that strain CMU-AB225T represents a novel species, namely Streptomyces venetus sp. nov. The type strain is CMU-AB225T (=JCM 31290T=TBRC 2001T).
['Sujarit|Kanaporn|K|', 'Kudo|Takuji|T|', 'Ohkuma|Moriya|M|', 'Pathom-Aree|Wasu|W|', 'Lumyong|Saisamorn|S|']
D028023:Arecaceae D015373:Bacterial Typing Techniques D001482:Base Composition D004269:DNA, Bacterial D003960:Diaminopimelic Acid D005227:Fatty Acids D005798:Genes, Bacterial D006017:Glycolipids D010743:Phospholipids D010802:Phylogeny D010858:Pigmentation D012336:RNA, Ribosomal, 16S D058441:Rhizosphere D017422:Sequence Analysis, DNA D012988:Soil Microbiology D013302:Streptomyces D013785:Thailand D024482:Vitamin K 2
Streptomyces blue aerial mycelium rhizosphere soil oil palm multilocus sequence analysis