Polyhydroxyalkanoate (PHBV) fibers obtained by a wet spinning method: Good in vitro cytocompatibility but absence of in vivo biocompatibility when used as a bone graft.
Polyhydroxyalkanoates (PHAs) are biomaterials widely investigated for tissue-engineering applications. In this regard, we describe a method to prepare fibers of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by a wet-spinning technique. Polymer fibers were used to test the cytocompatibility of the material in vitro. We have investigated their behavior in vitro in presence of the osteoblast-like (SaOs2) and macrophage (J774.2) cell lines. The PHBV fibers used were 100-200μm in diameter and offered a large surface for cell adhesion, similar to that they encounter when apposed onto a bone trabeculae. The fiber surface possessed a suitable roughness, a factor known to favor the adherence of cells, particularly osteoblasts. PHBV fibers were degraded in vitro by J774.2 cells as erosion pits were observable by transmission electron microscopy. The fibers were also colonisable by SaOs2 cells, which can spread and develop onto their surface. However, despite this good cytocompatibility observed in vitro, implantation in a bone defect drilled in rabbit femoral condyles showed that the material was only biotolerated without any sign of osteoconduction or degradation in vivo. We can conclude that PHBV is cytocompatible but is not suitable to be used as a bone graft as it does not favor osteoconduction and is not resorbed by bone marrow macrophages.
['Degeratu|C N|CN|', 'Mabilleau|G|G|', 'Aguado|E|E|', 'Mallet|R|R|', 'Chappard|D|D|', 'Cincu|C|C|', 'Stancu|I C|IC|']
D000818:Animals D001672:Biocompatible Materials D001861:Bone Regeneration D016025:Bone Transplantation D002448:Cell Adhesion D045744:Cell Line, Tumor D002470:Cell Survival D005269:Femur D006801:Humans D008422:Materials Testing D051379:Mice D023421:Models, Animal D010006:Osteoblasts D054813:Polyhydroxyalkanoates D011817:Rabbits D054457:Tissue Scaffolds
Cytocompatibility Bone graft Biomaterials Biopolymers Polyhydroxybutyrate