Comparison of the IHC, FISH, SISH and qPCR methods for the molecular             diagnosis of breast cancer.
Her2 proto-oncogene amplification and protein overexpression is observed in 20-40% of patients with breast cancer and plays a crucial role in invasive breast cancer and its treatment. In the present study, we investigated samples from 131 patients with invasive breast carcinoma. In all cases, the overexpression/amplification level of Her2 was determined using manual immunohistochemistry (IHC) and/or automatic IHC, fluorescence in situ hybridization (FISH), silver in situ hybridization (SISH) and quantitative polymerase chain reaction (qPCR). Using various methods, we demonstrated candidate methods for Her2 detection and their dependability. Our results demonstrate that these methods are highly comparable for the detection of Her2 overexpression/amplification. It was also revealed that qPCR is a valuable tool for the evaluation of Her2 gene overexpression/amplification. The results from pPCR analysis positively correlated with the results from IHC and FISH analysis. Moreover, in contrast to IHC or SISH/FISH, the results obtained by qPCR were not encumbered with any subjective error on the part of the evaluator.
['Tvrdík|D|D|', 'Staněk|L|L|', 'Skálová|H|H|', 'Dundr|P|P|', 'Velenská|Z|Z|', 'Povýšil|C|C|']
D000328:Adult D000368:Aged D001707:Biopsy, Needle D001943:Breast Neoplasms D004273:DNA, Neoplasm D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D007150:Immunohistochemistry D017404:In Situ Hybridization, Fluorescence D008875:Middle Aged D016133:Polymerase Chain Reaction D000090063:Proto-Oncogene Mas D018719:Receptor, ErbB-2 D015203:Reproducibility of Results D012680:Sensitivity and Specificity
Her2 immunohistochemistry fluorescence in situ hybridization silver in situ hybridization quantitative polymerase chain reaction breast  cancer