Recent improvements in sports drug testing concerning the initial testing for peptidic drugs (< 2 kDa) - sample preparation, mass spectrometric detection, and data review.
In this work, a novel initial testing assay based on liquid chromatography-mass spectrometry is presented, enabling the detection of peptidic drugs and drug candidates (< 2 kDa) prohibited in sports. The assay covers representatives and metabolites of gonadotropin releasing hormone and its analogs (GnRHs), growth hormone secretagogues (GHS), growth hormone releasing peptides (GHRPs), and the Vasopressin-analog Desmopressin. The general objective of this work was to reduce sample preparation efforts to a minimum while preserving highest possible sensitivity and specificity of the assay, demonstrating limits of detection between 50 and 200 pg/mL. Here, a "dilute-and-inject" strategy provides the simplest conceivable sample preparation procedure. Furthermore, the combination of well-established strategies for the determination of peptides, such as two-dimensional liquid chromatography, dimethyl sulfoxide (DMSO)-assisted electrospray ionization, high resolution mass spectrometric detection and a tailored reporter template, which facilitates data review enormously, provides a high-throughput initial testing assay for lower molecular mass peptidic and peptide-related analytes.
['Görgens|Christian|C|http://orcid.org/0000-0001-6061-0407', 'Guddat|Sven|S|', 'Thomas|Andreas|A|http://orcid.org/0000-0003-1199-0743', 'Thevis|Mario|M|http://orcid.org/0000-0002-1535-6451']
D002853:Chromatography, Liquid D003894:Deamino Arginine Vasopressin D004300:Doping in Sports D007987:Gonadotropin-Releasing Hormone D057166:High-Throughput Screening Assays D006801:Humans D057230:Limit of Detection D009842:Oligopeptides D013048:Specimen Handling D015813:Substance Abuse Detection D053719:Tandem Mass Spectrometry
two-dimensional liquid chromatography doping control high-resolution mass spectrometry peptide analysis