DNA methylation profiling to predict recurrence risk in stage Ι lung adenocarcinoma: Development and validation of a nomogram to clinical management.
Increasing evidence suggested DNA methylation may serve as potential prognostic biomarkers; however, few related DNA methylation signatures have been established for prediction of lung cancer prognosis. We aimed at developing DNA methylation signature to improve prognosis prediction of stage I lung adenocarcinoma (LUAD). A total of 268 stage I LUAD patients from the Cancer Genome Atlas (TCGA) database were included. These patients were separated into training and internal validation datasets. GSE39279 was used as an external validation set. A 13-DNA methylation signature was identified to be crucially relevant to the relapse-free survival (RFS) of patients with stage I LUAD by the univariate Cox proportional hazard analysis and the least absolute shrinkage and selection operator (LASSO) Cox regression analysis and multivariate Cox proportional hazard analysis in the training dataset. The Kaplan-Meier analysis indicated that the 13-DNA methylation signature could significantly distinguish the high- and low-risk patients in entire TCGA dataset, internal validation and external validation datasets. The receiver operating characteristic (ROC) analysis further verified that the 13-DNA methylation signature had a better value to predict the RFS of stage I LUAD patients in internal validation, external validation and entire TCGA datasets. In addition, a nomogram combining methylomic risk scores with other clinicopathological factors was performed and the result suggested the good predictive value of the nomogram. In conclusion, we successfully built a DNA methylation-associated nomogram, enabling prediction of the RFS of patients with stage I LUAD.
['Ma|Xianxiong|X|', 'Cheng|Jiancheng|J|', 'Zhao|Peng|P|', 'Li|Lei|L|', 'Tao|Kaixiong|K|0000-0002-7723-6121', 'Chen|Hengyu|H|0000-0001-9637-3992']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D019175:DNA Methylation D030541:Databases, Genetic D018572:Disease-Free Survival D005260:Female D020869:Gene Expression Profiling D015972:Gene Expression Regulation, Neoplastic D006801:Humans D053208:Kaplan-Meier Estimate D008175:Lung Neoplasms D008297:Male D008875:Middle Aged D015999:Multivariate Analysis D009364:Neoplasm Recurrence, Local D009367:Neoplasm Staging D049451:Nomograms D016016:Proportional Hazards Models D012372:ROC Curve D015203:Reproducibility of Results D012307:Risk Factors
DNA methylation nomogram signature LUAD recurrence-free survival