Intrinsically disordered inhibitor of glutamine synthetase is a functional protein with random-coil-like pKa values.
The sequential action of glutamine synthetase (GS) and glutamate synthase (GOGAT) in cyanobacteria allows the incorporation of ammonium into carbon skeletons. In the cyanobacterium Synechocystis sp. PCC 6803, the activity of GS is modulated by the interaction with proteins, which include a 65-residue-long intrinsically disordered protein (IDP), the inactivating factor IF7. This interaction is regulated by the presence of charged residues in both IF7 and GS. To understand how charged amino acids can affect the binding of an IDP with its target and to provide clues on electrostatic interactions in disordered states of proteins, we measured the pKa values of all IF7 acidic groups (Glu32, Glu36, Glu38, Asp40, Asp58, and Ser65, the backbone C-terminus) at 100 mM NaCl concentration, by using NMR spectroscopy. We also obtained solution structures of IF7 through molecular dynamics simulation, validated them on the basis of previous experiments, and used them to obtain theoretical estimates of the pKa values. Titration values for the two Asp and three Glu residues of IF7 were similar to those reported for random-coil models, suggesting the lack of electrostatic interactions around these residues. Furthermore, our results suggest the presence of helical structure at the N-terminus of the protein and of conformational changes at acidic pH values. The overall experimental and in silico findings suggest that local interactions and conformational equilibria do not play a role in determining the electrostatic features of the acidic residues of IF7.
['Cozza|Concetta|C|', 'Neira|José L|JL|', 'Florencio|Francisco J|FJ|', 'Muro-Pastor|M Isabel|MI|', 'Rizzuti|Bruno|B|']
D001426:Bacterial Proteins D004791:Enzyme Inhibitors D005974:Glutamate-Ammonia Ligase D064267:Intrinsically Disordered Proteins D000072417:Protein Domains D046939:Synechocystis
pKa values intrinsically disordered proteins electrostatics molecular dynamics titration nuclear magnetic resonance