Dietary Supplementation with Transgenic Camelina sativa Oil Containing 20:5n-3 and 22:6n-3 or Fish Oil Induces Differential Changes in the Transcriptome of CD3+ T Lymphocytes.
Eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3) are important for leukocyte function. This study investigated whether consuming transgenic Camelina sativa (tCSO) seed oil containing both 20:5n-3 and 22:6n-3 is as effective as fish oil (FO) for increasing the 20:5n-3 and 22:6n-3 content of leukocytes and altering mitogen-induced changes to the T cell transcriptome. Healthy adults (n = 31) consumed 450 mg/day of 20:5n-3 plus 22:6n-3 from either FO or tCSO for 8 weeks. Blood was collected before and after the intervention. 20:5n-3 and 22:6n-3 incorporation from tCSO into immune cell total lipids was comparable to FO. The relative expression of the transcriptomes of mitogen-stimulated versus unstimulated T lymphocytes in a subgroup of 16 women/test oil showed 4390 transcripts were differentially expressed at Baseline (59% up-regulated), 4769 (57% up-regulated) after FO and 3443 (38% up-regulated) after tCSO supplementation. The 20 most altered transcripts after supplementation differed between test oils. The most altered pathways were associated with cell proliferation and immune function. In conclusion, 20:5n-3 and 22:6n-3 incorporation into immune cells from tCSO was comparable to FO and can modify mitogen-induced changes in the T cell transcriptome, contingent on the lipid matrix of the oil.
['West|Annette L|AL|', 'Miles|Elizabeth A|EA|0000-0002-8643-0655', 'Han|Lihua|L|', 'Lillycrop|Karen A|KA|', 'Napier|Johnathan A|JA|0000-0003-3580-3607', 'Calder|Philip C|PC|', 'Burdge|Graham C|GC|0000-0002-7665-2967']
D000293:Adolescent D000328:Adult D000368:Aged D019607:Brassicaceae D017252:CD3 Complex D019587:Dietary Supplements D004281:Docosahexaenoic Acids D015118:Eicosapentaenoic Acid D005260:Female D005395:Fish Oils D064368:Healthy Volunteers D006801:Humans D008297:Male D008875:Middle Aged D010938:Plant Oils D013601:T-Lymphocytes D059467:Transcriptome D055815:Young Adult
fish oil T lymphocyte eicosapentaenoic acid docosahexaenoic acid RNAseq omega-3 transgenic plant