Expression of Histone Deacetylases HDAC1 and HDAC2 and Their Role in Apoptosis in the Penumbra Induced by Photothrombotic Stroke.
In ischemic stroke, vascular occlusion rapidly induces tissue infarct. Over the ensuing hours, damage spreads to adjacent tissue and forms transition zone (penumbra), which is potentially salvageable. Epigenetic regulation of chromatin structure controls gene expression and protein synthesis. We studied the expression of histone deacetylases HDAC1 and HDAC2 in the penumbra at 4 or 24 h after photothrombotic stroke (PTS) in the rat brain cortex. PTS increased the expression of HDAC1 and HDAC2 in penumbra and caused the redistribution of HDAC1 but not HDAC2 from the neuronal nuclei to cytoplasm. In astrocytes, HDAC1 expression and localization did not change. In neurons, HDAC2 localized exclusively in nuclei, but in astrocytes, it was also observed in processes. PTS induced neuronal apoptosis in the penumbra. TUNEL-stained apoptotic neurons co-localized with HDAC2 but not HDAC1. These data suggest that HDAC2 may represent the potential target for anti-stroke therapy and its selective inhibition may be a promising strategy for the protection of the penumbra tissue after ischemic stroke.
['Demyanenko|S V|SV|', 'Dzreyan|V A|VA|', 'Neginskaya|M A|MA|', 'Uzdensky|A B|AB|https://orcid.org/0000-0002-0344-434X']
D000818:Animals D017209:Apoptosis D001253:Astrocytes D015415:Biomarkers D002540:Cerebral Cortex D005904:Glial Fibrillary Acidic Protein D056284:Histone Deacetylase 1 D056464:Histone Deacetylase 2 D008027:Light D008297:Male D017208:Rats, Wistar D020521:Stroke D013927:Thrombosis
Histone deacetylase Apoptosis Penumbra Stroke