CD4+ CD8αα+ T cells in the gastric epithelium mediate chronic inflammation induced by Helicobacter felis.
CD4+ CD8αα+ double-positive intraepithelial T lymphocytes (DP T cells), a newly characterized subset of intraepithelial T cells, are reported to contribute to local immunosuppression. However, the presence of DP T cells in Helicobacter. pylori -induced gastritis and their relationship with disease prognosis has yet to be elucidated. In this study, a chronic gastritis model was established by infecting mice with Helicobacter felis. Gastric-infiltrating lymphocytes were isolated from these mice and analyzed by flow cytometry. The frequency of DP T cells in H. felis-induced gastritis mice was higher than that in uninfected mice. The gastric DP T cells were derived from lamina propria cells but were predominantly distributed in the gastric epithelial layer. These gastric DP T cells also exhibited anti-inflammatory functions, and they inhibited the maturation of dendritic cells and proliferation of CD4+ T lymphocytes in vitro. Elimination of DP T cells simultaneously resulted in severe gastritis and a reduction of H. felis load in vivo. Finally, vaccine mixed with different adjuvants was used to explore the relationship between vaccine efficacy and DP cells. Silk fibroin as the vaccine delivery system enhanced vaccine efficacy by reducing the number of DP T cells. This study demonstrated that DP T cells perform an immunosuppressive role in Helicobacter felis-induced gastritis, and consequently, DP T cells may affect disease prognosis and vaccine efficacy.
['Ruan|Guojing|G|', 'Huang|An|A|', 'Hu|Chupeng|C|', 'Xu|Ningyin|N|', 'Fan|Menghui|M|', 'Zhang|Zhenxing|Z|', 'Wang|Yue|Y|', 'Xing|Yingying|Y|']
D000818:Animals D015496:CD4-Positive T-Lymphocytes D004848:Epithelium D005753:Gastric Mucosa D044922:Helicobacter felis D007249:Inflammation D051379:Mice D013601:T-Lymphocytes
Helicobacter felis Chronic gastritis CD4+CD8αα+ T cells Vaccine adjuvant