Rapid strand replacement primer thermostat visual sensor based on Bst DNA polymerase and pyrophosphatase for detecting Vibrio parahaemolyticus.
Vibrio parahaemolyticus is a major hidden danger of food safety. To develop a rapid, sensitive and on-site detecting method of Vibrio parahaemolyticus (V. parahaemolyticus), a strand replacement primer thermostat phosphate (SRPP) visual sensor was proposed, based on Bst DNA polymerase and pyrophosphatase. The novel strand replacement primer (SRP) facilitates chain substitution and to open a self-folding hairpin by adding region at its 3' end. Under the action of the SRP, a pair of external primers and two inner primers, target DNA is specifically amplified at 63 °C relies mainly on the hairpin. Many pyrophosphates (PPi) are simultaneously generated as by-products, which can be converted into phosphates (Pi) by pyrophosphatase for phosphomolybdate blue visual detection within 5 min. The proposed biosensor can detect 1.29 × 103 copies of V. parahaemolyticus within 35 min.
['Li|Xuetong|X|', 'Su|Yuan|Y|', 'Chu|Huashuo|H|', 'Lyu|Shuxia|S|', 'Tian|Jingjing|J|', 'Xu|Wentao|W|']
D017931:DNA Primers D004259:DNA-Directed DNA Polymerase D005504:Food Analysis D005516:Food Microbiology D011755:Pyrophosphatases D012680:Sensitivity and Specificity D014736:Vibrio parahaemolyticus
Strand replacement primer Visualization Pyrophosphatase Vibrio parahaemolyticus Phosphate