Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz radiofrequency fields.
PURPOSE
To assess 1.9 GHz radiofrequency (RF) field exposure on gene expression within a variety of discrete mouse brain regions using whole genome microarray analysis.


MATERIALS AND METHODS
Adult male C57BL/6 mice were exposed to 1.9 GHz pulse-modulated or continuous-wave RF fields for 4 h/day for 5 consecutive days at whole body average (WBA) specific absorption rates of 0 (sham), ∼0.2 W/kg and ∼1.4 W/kg. Total RNA was isolated from the auditory cortex, amygdala, caudate, cerebellum, hippocampus, hypothalamus, and medial prefrontal cortex and differential gene expression was assessed using Illumina MouseWG-6 (v2) BeadChip arrays. Validation of potentially responding genes was conducted by RT-PCR.


RESULTS
When analysis of gene expression was conducted within individual brain regions when controlling the false discovery rate (FDR), no differentially expressed genes were identified relative to the sham control. However, it must be noted that most fold changes among groups were observed to be less than 1.5-fold and this study had limited ability to detect such small changes. While some genes were differentially expressed without correction for multiple-comparisons testing, no consistent pattern of response was observed among different RF-exposure levels or among different RF-modulations.


CONCLUSIONS
The current study provides the most comprehensive analysis of potential gene expression changes in the rodent brain in response to RF field exposure conducted to date. Within the exposure conditions and limitations of this study, no convincing evidence of consistent changes in gene expression was found in response to 1.9 GHz RF field exposure.
['McNamee|James P|JP|', 'Bellier|Pascale V|PV|', 'Konkle|Anne T M|AT|', 'Thomas|Reuben|R|', 'Wasoontarajaroen|Siriwat|S|', 'Lemay|Eric|E|', 'Gajda|Greg B|GB|']
D065638:Absorption, Radiation D000818:Animals D001921:Brain D004307:Dose-Response Relationship, Radiation D005786:Gene Expression Regulation D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D008872:Microwaves D009419:Nerve Tissue Proteins D011829:Radiation Dosage D014018:Tissue Distribution D014916:Whole-Body Irradiation
Gene expression mouse brain radiofrequency microarray RT-PCR