Biofilm formation by Candida albicans is inhibited by photodynamic antimicrobial chemotherapy (PACT), using chlorin e6: increase in both ROS production and membrane permeability.
Candida albicans is an opportunistic fungal producing both superficial and systemic infections in immunocompromised patients. Furthermore, it has been described an increase in the frequency of infections which have become refractory to standard antifungal therapy. Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a nontoxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells. The objective of this study was to investigate the effects of PACT, using chlorin e6, as a photosensitizer on C. albicans. In this work, we studied the effect of PACT on both cell growth and biofilm formation by C. albicans. In addition, both ROS production and cell permeability were determined after PACT. PACT inhibited both growth and biofilm formation by C. albicans. We have also observed that PACT increased both ROS production (six times) and cell membrane permeability (five times) in C. albicans. PACT decreased both cell growth and biofilm development. The effect of PACT using chlorin e6 on C. albicans could be associated with an increase in ROS production, which could increase cell permeability, producing permanent damage to the cell membranes, leading to the cell death.
['Carvalho|Moisés Lopes|ML|', 'Pinto|Ana Paula|AP|', 'Raniero|Leandro José|LJ|', 'Costa|Maricilia Silva|MS|']
D000890:Anti-Infective Agents D018441:Biofilms D002176:Candida albicans D002463:Cell Membrane Permeability D002735:Chlorophyllides D008826:Microbial Sensitivity Tests D017319:Photosensitizing Agents D011166:Porphyrins D017382:Reactive Oxygen Species
Candida albicans Photodynamic antimicrobial chemotherapy PACT Chlorin e6