Evaluation radioprotective effect of curcumin conjugated albumin nanoparticles.
In this research, curcumin (CUR) conjugated albumin based nanoparticles (BSA-CUR) were designed for improvement and evaluation radioprotective effect of CUR. In this way, we have prepared BSA-CUR by covalently binding the CUR with BSA. Next, this synthesized prodrug was evaluated for physical and chemical properties by Fourier-transform infrared (FTIR), Dynamic light scattering (DLS), Transmission electron microscopy (TEM), Ultraviolet-visible (UV/Vis), and Differential scanning calorimetry (DSC) analysis. Furthermore, the chemical stability of designed prodrug was appraised. The result shows that the size of nanoparticles is 174.4 nm with a polydispersity index (PdI) of 0.191. The nanoparticles have a high loading capacity and show sustained release behavior. Loading of CUR to BSA not only could increase the chemical stability of CUR, but also could improve radioprotection efficacy of it's against X-Ray irradiation. The HHF-2 cells show 107% viability in the presence of BSA-CUR at a concentration of 50 µg/mL, whereas non-treated cells show 46% viability, under X-Ray irradiation. Also in vivo study results show that, four out of five mice have died when the mice irradiated by X-Ray and no received any treatment. Although, for a group that treated with BSA-CUR and also irradiated by X-Ray, median survival and survival rate was higher than CUR treated and control mice, and only two out of five mice have died. The result of this study proved that BSA-CUR can be used as a proficient vehicle for improving the potential radioprotective effect of CUR.
['Nosrati|Hamed|H|', 'Danafar|Hossein|H|', 'Rezaeejam|Hamed|H|', 'Gholipour|Nazila|N|', 'Rahimi-Nasrabadi|Mehdi|M|']
D000818:Animals D002417:Cattle D002460:Cell Line D002470:Cell Survival D003474:Curcumin D004337:Drug Carriers D006461:Hemolysis D006801:Humans D008807:Mice, Inbred BALB C D053758:Nanoparticles D011355:Prodrugs D011837:Radiation-Protective Agents D012710:Serum Albumin, Bovine D014965:X-Rays
Radioprotection Curcumin BSA X-Ray irradiation Drug conjugate