IDH1 Associated with Neuronal Apoptosis in Adult Rats Brain Following Intracerebral Hemorrhage.
Isocitrate dehydrogenase 1 (IDH1), one member of the IDH family can convert isocitrate to α-ketoglutarate (α-KG) via oxidative decarboxylation. IDH1 and IDH2 mutations have been identified in multiple tumor types and the mutations confer neomorphic activity in the mutant protein, resulting in the conversion of α-KG to the oncometabolite, D-2-hydroxyglutarate (2-HG). The subsequent accumulation of 2-HG results in epigenetic dysregulation via inhibition of α-KG-dependent histone and DNA demethylase. And the glutamate levels are reduced in IDH mutant cells compared to wild-type. We have known that diffuse gliomas contain a high frequency of mutations in the IDH1 gene. However, the expression of IDH1 and its roles in Intracranial hemorrhage (ICH) remain largely unknown. We observed increased expression of IDH1 in neurons after intracerebral hemorrhage. Up-regulation of IDH1 was found to be accompanied by the increased expression of active caspase-3 and pro-apoptotic Bcl-2-associated X protein and decreased expression of anti-apoptotic protein B cell lymphoma-2 in vivo and vitro studies. So we hypothesized that IDH1 was involved in the regulation of neuronal apoptosis. The present research for the first time detected the expression and variation of IDH1 surrounding the hematoma, and all data proved the involvement of IDH1 in neuronal apoptosis following ICH.
['Chen|Xing|X|', 'Wang|Hongmei|H|', 'Yu|Weibing|W|', 'Chen|Fen|F|', 'Wang|Guiyun|G|', 'Shi|Jiajia|J|', 'Zhou|Chunying|C|']
D000375:Aging D000818:Animals D017209:Apoptosis D001522:Behavior, Animal D015415:Biomarkers D015153:Blotting, Western D001921:Brain D002543:Cerebral Hemorrhage D004195:Disease Models, Animal D007521:Isocitrate Dehydrogenase D008297:Male D009474:Neurons D016716:PC12 Cells D051381:Rats D017207:Rats, Sprague-Dawley
IDH1 Neuron Apoptosis ICH Active caspase-3