Resolving in vivo gene expression during collective cell migration using an integrated RNAscope, immunohistochemistry and tissue clearing method.
During collective cell migration individual cells display diverse behaviors that complicate our understanding of group cell decisions of direction and cohesion. In vivo gene and protein expression analyses would shed light on the underlying molecular choreography. However, this information has been limited due to difficulties to integrate single cell detection methods and the simultaneous readout of multiple signals deep within the embryo. Here, we optimize and integrate multiplex fluorescence in situ hybridization by RNAscope, immunohistochemistry, and tissue clearing to visualize transcript and protein localization within single cells deep within intact chick embryos. Using standard confocal microscopy, we visualize the mRNA expression of up to 3 genes simultaneously within protein labeled HNK1-positive migrating cranial neural crest cells within 2day old cleared chick embryos. Gene expression differences measured between adjacent cells or within subregions are quantified using spot counting and polyline kymograph methods, respectively. This optimization and integration of methods provide an improved 3D in vivo molecular interrogation of collective cell migration and foundation to broaden into a wider range of embryo and adult model systems.
['Morrison|Jason A|JA|', 'McKinney|Mary Cathleen|MC|', 'Kulesa|Paul M|PM|']
D000818:Animals D002465:Cell Movement D002642:Chick Embryo D018507:Gene Expression Regulation, Developmental D007150:Immunohistochemistry D017404:In Situ Hybridization, Fluorescence D018613:Microscopy, Confocal D009432:Neural Crest D015398:Signal Transduction D059467:Transcriptome
RNAscope Immunohistochemistry Tissue clearing Multiplex Chick FRUIT