Processing of Cryo-EM Movie Data.
Direct detector device (DDD) cameras dramatically enhance the capabilities of electron cryomicroscopy (cryo-EM) due to their improved detective quantum efficiency (DQE) relative to other detectors. DDDs use semiconductor technology that allows micrographs to be recorded as movies rather than integrated individual exposures. Movies from DDDs improve cryo-EM in another, more surprising, way. DDD movies revealed beam-induced specimen movement as a major source of image degradation and provide a way to partially correct the problem by aligning frames or regions of frames to account for this specimen movement. In this chapter, we use a self-consistent mathematical notation to explain, compare, and contrast several of the most popular existing algorithms for computationally correcting specimen movement in DDD movies. We conclude by discussing future developments in algorithms for processing DDD movies that would extend the capabilities of cryo-EM even further.
['Ripstein|Z A|ZA|', 'Rubinstein|J L|JL|']
D000465:Algorithms D020285:Cryoelectron Microscopy D007091:Image Processing, Computer-Assisted D021621:Imaging, Three-Dimensional D009038:Motion D012666:Semiconductors D013048:Specimen Handling
Cryo-EM Movie Direct detector device Camera Algorithm Beam-induced movement Data processing Direct electron detector High-resolution Motion correction cryoEM