Longitudinal monitoring adipose-derived stem cell survival by PET imaging hexadecyl-4-¹²⁴I-iodobenzoate in rat myocardial infarction model.
This study aims to monitor how the change of cell survival of transplanted adipose-derived stem cells (ADSCs) responds to myocardial infarction (MI) via the hexadecyl-4-(124)I-iodobenzoate ((124)I-HIB) mediated direct labeling method in vivo. Stem cells have shown the potential to improve cardiac function after MI. However, monitoring of the fate of transplanted stem cells at target sites is still unclear. Rat ADSCs were labeled with (124)I-HIB, and radiolabeled ADSCs were transplanted into the myocardium of normal and MI model. In the group of (124)I-HIB-labeled ADSC transplantation, in vivo imaging was performed using small-animal positron emission tomography (PET)/computed tomography (CT) for 9 days. Twenty-one days post-transplantation, histopathological analysis and apoptosis assay were performed. ADSC viability and differentiation were not affected by (124)I-HIB labeling. In vivo tracking of the (124)I-HIB-labeled ADSCs was possible for 9 and 3 days in normal and MI model, respectively. Apoptosis of transplanted cells increased in the MI model compared than that in normal model. We developed a direct labeling agent, (124)I-HIB, and first tried to longitudinally monitor transplanted stem cell to MI. This approach may provide new insights on the roles of stem cell monitoring in living bodies for stem cell therapy from pre-clinical studies to clinical trials.
['Kim|Min Hwan|MH|', 'Woo|Sang-Keun|SK|', 'Lee|Kyo Chul|KC|', 'An|Gwang Il|GI|', 'Pandya|Darpan|D|', 'Park|Noh Won|NW|', 'Nahm|Sang-Soep|SS|', 'Eom|Ki Dong|KD|', 'Kim|Kwang Il|KI|', 'Lee|Tae Sup|TS|', 'Kim|Chan Wha|CW|', 'Kang|Joo Hyun|JH|', 'Yoo|Jeongsoo|J|', 'Lee|Yong Jin|YJ|']
D017667:Adipocytes D000273:Adipose Tissue D000818:Animals D017209:Apoptosis D002470:Cell Survival D017690:Cell Transplantation D004195:Disease Models, Animal D019788:Fluorodeoxyglucose F18 D007457:Iodine Radioisotopes D007463:Iodobenzoates D008297:Male D009203:Myocardial Infarction D009206:Myocardium D049268:Positron-Emission Tomography D019275:Radiopharmaceuticals D051381:Rats D017207:Rats, Sprague-Dawley D020133:Reverse Transcriptase Polymerase Chain Reaction D033581:Stem Cell Transplantation D013234:Stem Cells D014057:Tomography, X-Ray Computed
Adipose-derived stem cells Myocardial infarction Hexadecyl-4-(124)I-iodobenzoate Direct labeling agent Cell tracking