Blood CD9+ B cell, a biomarker of bronchiolitis obliterans syndrome after lung transplantation.
Bronchiolitis obliterans syndrome is the main limitation for long-term survival after lung transplantation. Some specific B cell populations are associated with long-term graft acceptance. We aimed to monitor the B cell profile during early development of bronchiolitis obliterans syndrome after lung transplantation. The B cell longitudinal profile was analyzed in peripheral blood mononuclear cells from patients with bronchiolitis obliterans syndrome and patients who remained stable over 3 years of follow-up. CD24hi CD38hi transitional B cells were increased in stable patients only, and reached a peak 24 months after transplantation, whereas they remained unchanged in patients who developed a bronchiolitis obliterans syndrome. These CD24hi CD38hi transitional B cells specifically secrete IL-10 and express CD9. Thus, patients with a total CD9+ B cell frequency below 6.6% displayed significantly higher incidence of bronchiolitis obliterans syndrome (AUC = 0.836, PPV = 0.75, NPV = 1). These data are the first to associate IL-10-secreting CD24hi CD38hi transitional B cells expressing CD9 with better allograft outcome in lung transplant recipients. CD9-expressing B cells appear as a contributor to a favorable environment essential for the maintenance of long-term stable graft function and as a new predictive biomarker of bronchiolitis obliterans syndrome-free survival.
['Brosseau|Carole|C|0000-0002-3557-1538', 'Danger|Richard|R|', 'Durand|Maxim|M|', 'Durand|Eugénie|E|', 'Foureau|Aurore|A|', 'Lacoste|Philippe|P|', 'Tissot|Adrien|A|', 'Roux|Antoine|A|', 'Reynaud-Gaubert|Martine|M|', 'Kessler|Romain|R|', 'Mussot|Sacha|S|', 'Dromer|Claire|C|', 'Brugière|Olivier|O|', 'Mornex|Jean François|JF|', 'Guillemain|Romain|R|', 'Claustre|Johanna|J|', 'Magnan|Antoine|A|', 'Brouard|Sophie|S|', '|||']
D000293:Adolescent D000328:Adult D000368:Aged D001402:B-Lymphocytes D015415:Biomarkers D001989:Bronchiolitis Obliterans D015331:Cohort Studies D005260:Female D005500:Follow-Up Studies D006084:Graft Rejection D006085:Graft Survival D006801:Humans D007963:Leukocytes, Mononuclear D016040:Lung Transplantation D008297:Male D008875:Middle Aged D011183:Postoperative Complications D011379:Prognosis D012307:Risk Factors D015996:Survival Rate D013577:Syndrome D060245:Tetraspanin 29 D014184:Transplantation, Homologous D055815:Young Adult
biomarker B cell biology basic (laboratory) research/science bronchiolitis obliterans (BOS) lung transplantation/pulmonology translational research/science