Gas chromatographic analysis of fatty acid methyl esters of milk fat by an ionic liquid derived from L-phenylalanine as the stationary phase.
A Gas Chromatography (GC) method has been developed for the separation and characterization of the different fatty acids in anhydrous milk fat (AMF) by means of an ionic liquid stationary phase, characterized by a monocationic imidazolium salt derived from L-phenylalanine. The inner surface of a fused silica capillary column was modified using this ionic liquid functionality and 3-aminopropyldiethoxymethyl silane. This coated GC column, which exhibited good thermal stability (270°C) and good efficiency (2700 plates/m), has been characterized using the Abraham solvation parameter model. The intra-day and inter-day precision of the method have been evaluated, obtaining relative standard deviations (RSD) from 0.99% to 4.0% and from 2.8% to 9.2%, respectively. Furthermore, recoveries from 90% and 99% have been achieved.
['Mendoza|Laura González|LG|', 'González-Álvarez|Jaime|J|', 'Gonzalo|Carla Fernández|CF|', 'Arias-Abrodo|Pilar|P|', 'Altava|Belén|B|', 'Luis|Santiago V|SV|', 'Burguete|Maria Isabel|MI|', 'Gutiérrez-Álvarez|María Dolores|MD|']
D000818:Animals D002849:Chromatography, Gas D004355:Drug Stability D004952:Esters D005227:Fatty Acids D052578:Ionic Liquids D008892:Milk D010649:Phenylalanine D015203:Reproducibility of Results D012997:Solvents D013696:Temperature
Fatty acids Ionic liquids Stationary phases Gas chromatography Solvation parameter model