A potential anti-inflammation activity and depigmentation effect of Lespedeza bicolor extract and its fractions.
Postinflammatory hyperpigmentation (PIH) is an acquire hypermelanosis after cutaneous inflammation and injury. The aim of the present study was to investigate a natural ingredient with the anti-inflammatory and depigmentation activities into possible applications of postinflammatory hyperpigmentation. Methanol extracts of Lespedeza bicolor and its various fractions inhibited LPS-induced NO production in RAW 264.7 macrophages in a concentration-dependent manner. In particular, the ethyl acetate fraction was shown to be inhibition of NO production (89%) and down-regulation of iNOS mRNA without causing cytotoxicity. In addition, ethyl acetate fraction significantly attenuated LPS-induced NF-κB activation (P < 0.05), indicating the anti-inflammatory activity due to NF-κB inhibition. Moreover, extracts, mainly ethyl acetate fraction, exhibited not only DPPH free radical scavenging activity (IC50, 112.45 μg/mL) with 4 times lower activity than ascorbic acid, but also anti-tyrosinase activity (IC50, 1 μg/mL) with a similar activity to arbutin showing a competitive inhibitor. Furthermore, vitexin and haginins A, B and C were identified through LC-MS analysis as potential compounds responsible for these effects. These results suggest that L. bicolor extract have anti-inflammatory, antioxidant activities and tyrosinase inhibitory effect and it might be used in the management of postinflammatory pigmentation through inhibition of pathogenic process involved in hyperpigmentation.
['Lee|Seung Jin|SJ|', 'Hossaine|M D Akil|MD|', 'Park|Seung Chun|SC|']
Anti-inflammation Depigmentation Lespedeza bicolor iNOS NF-κB Tyrosinase activity