Biomineralization improves mechanical and osteogenic properties of multilayer-modified PLGA porous scaffolds.
Poly-(lactide-co-glycolide acid) (PLGA) has been widely investigated as scaffold material for bone tissue engineering owing to its biosafety, biodegradability, and biocompatibility. However, the bioinert surface of PLGA may fail in regulating cellular behavior and directing osteointegration between the scaffold and the host tissue. In this article, oxidized chondroitin sulfate (oCS) and type I collagen (Col I) were assembled onto PLGA surface via layer by layer technique (LbL) as an adhesive coating for the attachment of inorganic minerals. The multilayer-modified PLGA scaffold was mineralized in vitro to ensure the deposition of nanohydroxyapatite (nHAP). It was found that nHAP crystals were more uniformly and firmly attached on the multilayer-modified PLGA as compared with the pure PLGA scaffold, which remarkably improved PLGA surface and mechanical properties. Additionally, in vitro biocompatibility of PLGA scaffold, in terms of bone mesenchymal stem cells (BMSCs) attachment, spreading and proliferation was greatly enhanced by nHAP coating and multilayer deposition. Furthermore, the fabricated composite scaffold also shows the ability to promote the osteogenic differentiation of BMSCs through the up-regulation of osteogenic marker genes. Thus, this novel biomimetic composite scaffold might achieve a desirable therapeutic result for bone tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2714-2725, 2018.
['Kong|Junchao|J|', 'Wei|Bo|B|', 'Groth|Thomas|T|', 'Chen|Zhuming|Z|', 'Li|Lihua|L|', 'He|Dongning|D|', 'Huang|Rui|R|', 'Chu|Jiaqi|J|', 'Zhao|Mingyan|M|']
D000818:Animals D015415:Biomarkers D000077320:Biomineralization D002448:Cell Adhesion D049109:Cell Proliferation D005260:Female D059630:Mesenchymal Stem Cells D010012:Osteogenesis D000077182:Polylactic Acid-Polyglycolic Acid Copolymer D016062:Porosity D017207:Rats, Sprague-Dawley D013552:Swine D013818:Thermogravimetry D054457:Tissue Scaffolds
biomineralization Poly-(lactide-co-glycolide acid) bone tissue engineering layer by layer technique mesenchymal stem cells