Hericium Erinaceus Prevents DEHP-Induced Mitochondrial Dysfunction and Apoptosis in PC12 Cells.
Hericium Erinaceus (HE) is a medicinal plant known to possess anticarcinogenic, antibiotic, and antioxidant activities. It has been shown to have a protective effect against ischemia-injury-induced neuronal cell death in rats. As an extending study, here we examined in pheochromocytoma 12 (PC12) cells, whether HE could exert a protective effect against oxidative stress and apoptosis induced by di(2-ethylhexyl)phthalate (DEHP), a plasticizer known to cause neurotoxicity. We demonstrated that pretreatment with HE significantly attenuated DEHP induced cell death. This protective effect may be attributed to its ability to reduce intracellular reactive oxygen species levels, preserving the activity of respiratory complexes and stabilizing the mitochondrial membrane potential. Additionally, HE pretreatment significantly modulated Nrf2 and Nrf2-dependent vitagenes expression, preventing the increase of pro-apoptotic and the decrease of anti-apoptotic markers. Collectively, our data provide evidence of new preventive nutritional strategy using HE against DEHP-induced apoptosis in PC12 cells.
['Amara|Ines|I|', 'Scuto|Maria|M|0000-0003-1019-5158', 'Zappalà|Agata|A|', 'Ontario|Maria Laura|ML|', 'Petralia|Antonio|A|', 'Abid-Essefi|Salwa|S|', 'Maiolino|Luigi|L|0000-0002-5295-7221', 'Signorile|Anna|A|', 'Trovato Salinaro|Angela|A|', 'Calabrese|Vittorio|V|0000-0002-0478-985X']
D000818:Animals D017209:Apoptosis D053148:Caspase 3 D004051:Diethylhexyl Phthalate D045222:Electron Transport Chain Complex Proteins D018840:HSP70 Heat-Shock Proteins D051547:Heme Oxygenase-1 D000084824:Hericium D053078:Membrane Potential, Mitochondrial D008928:Mitochondria D051267:NF-E2-Related Factor 2 D016716:PC12 Cells D010936:Plant Extracts D051381:Rats D017382:Reactive Oxygen Species D056564:Sirtuin 1 D013879:Thioredoxins D016159:Tumor Suppressor Protein p53 D051028:bcl-2-Associated X Protein
Hericium erinaceus apoptosis oxidative stress vitagenes Di (2-Ethylhexyl) pthalate mitochondrial respiratory complexes