MicroRNA (miRNA) Profiling.
MicroRNAs (miRNAs) are small, highly conserved noncoding RNA molecules involved in the regulation of gene expression. Since each miRNA regulates the expression of hundreds of target mRNAs, miRNAs could function as master coordinators, efficiently regulating fundamental cellular processes, including proliferation, apoptosis, and development. Furthermore, miRNAs may provide useful diagnostic and therapeutic targets in a variety of diseases. However, miRNA expression profiling is essential for the investigation of the biological functions and clinical applications of miRNAs. Therefore, in this chapter, we review and discuss commonly used techniques for miRNAs profiling, as well as their advantages and restrictions.
['Gao|Lu|L|', 'Jiang|Feng|F|']
D000818:Animals D020869:Gene Expression Profiling D015723:Gene Library D059014:High-Throughput Nucleotide Sequencing D006801:Humans D035683:MicroRNAs D020411:Oligonucleotide Array Sequence Analysis D016133:Polymerase Chain Reaction
miRNA DDPCR Microarray