Qualitative and quantitative analysis of seven oligosaccharides in Morinda officinalis using double-development HPTLC and scanning densitometry.
The quality of Morindaofficinalis, which has been used as a Yang-tonic agent for a long time in China, can be evaluated. A double-development high performance thin layer chromatography (HPTLC) method has been established to simultaneously analyze quality and quantity of seven inulin-type oligosaccharides (DP=3-9) in Morindaofficinalis. The chromatography was performed on a silica gel 60 plate with the 7:5:2:1 proportion (v/v) of n-butanol-isopropanol-water-acetic acid for the first and second developments, respectively. The bands were visualized by the reaction with aniline-diphenylamine-phosphoric acid solution and analyzed by densitometric TLC at 540 nm. Quantification of seven oligosaccharides was achieved by densitometry at 540 nm. The investigated standard sugar had good linearity (R2>0.99) within test ranges. The amounts of seven oligosaccharides were calculated by the relative correction factor (RCF). Therefore, the developed TLC method could be used for quality control of Morindaofficinalis.
['Zhou|Bin|B|', 'Chang|Jun|J|', 'Wang|Ping|P|', 'Li|Jie|J|', 'Cheng|Dan|D|', 'Zheng|Peng-Wu|PW|']
D020001:1-Butanol D019840:2-Propanol D019342:Acetic Acid D002138:Calibration D002681:China D002851:Chromatography, High Pressure Liquid D002855:Chromatography, Thin Layer D003720:Densitometry D032066:Morinda D009844:Oligosaccharides D010936:Plant Extracts D011786:Quality Control D012044:Regression Analysis D015203:Reproducibility of Results D012822:Silicon Dioxide D013696:Temperature D014867:Water
oligosaccharide Morindaofficinalis TLC Quantitative determination