Identification of an alkaline lipase capable of better enrichment of EPA than DHA due to fatty acids selectivity and regioselectivity.
The whole genome of Streptomyces violascens (=ATCC 27968) was sequenced and the cloning and expression of OUC-Lipase 6 were conducted in Bacillus subtilis WB800. The recombinant enzyme belongs to the lipolytic enzymes family V. OUC-Lipase 6 showed optimal activity at 30 °C and pH 9.0, and retained 90.2% of its activity in an alkaline buffer (pH 8.0, 30 °C and 96 h). OUC-Lipase 6 showed good stability under medium temperature conditions (residual activity of 68.8%, pH 8.0, 45 °C and 96 h). OUC-Lipase 6 could selectively hydrolyze fatty acids on the glyceride backbone, thus improving the contents of DHA and EPA in codfish oil. OUC-Lipase 6 also showed regioselectivity, resulting in a better enrichment efficiency for EPA than DHA. After hydrolyzing for 36 h via OUC-Lipase 6, the contents of EPA and DHA were improved to 3.24-fold and 1.98-fold, respectively.
['Gao|Kunpeng|K|', 'Chu|Wenqin|W|', 'Sun|Jianan|J|', 'Mao|Xiangzhao|X|']
D004281:Docosahexaenoic Acids D015118:Eicosapentaenoic Acid D016680:Genome, Bacterial D005989:Glycerides D006868:Hydrolysis D008049:Lipase D013302:Streptomyces D013379:Substrate Specificity
Lipase EPA Fatty acids selectivity Regioselectivity Streptomyces violascens Fish oil Genome sequence