Application of Albumin-embedded Magnetic Nanoheaters for Release of Etoposide in Integrated Chemotherapy and Hyperthermia of U87-MG Glioma Cells.
BACKGROUND/AIM
Malignant gliomas remain refractory to several therapeutic approaches and the requirement for novel treatment modalities is critical to combat this disease. Etoposide is a topoisomerase-II inhibitor, which promotes DNA damage and apoptosis of cancer cells. In this study, we prepared albumin with embedded magnetic nanoparticles and etoposide for in vitro evaluation of combined hyperthermia and chemotherapy.


MATERIAL AND METHODS
Magnetic nanoparticles were prepared by a modified co-precipitation method in the presence of human serum albumin and etoposide. A cellular proliferation assay was used to determine the effects of these nanostructures on the viability of U87 glioma cells in an alternating magnetic field.


RESULTS
The in vitro experiments showed that cell viability decreased to 59.4% after heat treatment alone and to 53.8% on that with free etoposide, while combined treatment resulted in 7.8% cell viability.


CONCLUSION
Integrating hyperthermia and chemotherapy using albumin co-embedded magnetic nanoheaters and etoposide may represent a promising therapeutic option for glioblastoma.
['Babincová|Melánia|M|', 'Vrbovská|Hana|H|', 'Sourivong|Paul|P|', 'Babinec|Peter|P|', 'Durdík|Štefan|Š|']
D001932:Brain Neoplasms D045744:Cell Line, Tumor D003131:Combined Modality Therapy D004337:Drug Carriers D004354:Drug Screening Assays, Antitumor D005047:Etoposide D005910:Glioma D006801:Humans D006979:Hyperthermia, Induced D008280:Magnetics D015394:Molecular Structure D053758:Nanoparticles D000075462:Serum Albumin, Human D059005:Topoisomerase II Inhibitors
etoposide chemotherapy hyperthermia magnetic nanoparticles human serum albumin Glioblastoma