An effective method for the determination of the enantio-purity of L-α-glycerophosphocholine (L-α-GPC).
Herein we describe for the first time a practical analytical method for determining the enantio-purity of L-α-glycerophosphocholine (L-α-GPC) by chiral derivatization. The use of a suitable chiral boronic acid allowed the formation of a mixture of two diastereomeric boronate esters whose ratio, carefully analyzed by 1HNMR spectroscopy, reflects L-α-GPC enantiomeric excesses. The determination of the enantiopurity of L-α-GPC furnishes convincing correlations with its manufacturing process.
['de Ferra|Lorenzo|L|', 'Massa|Antonio|A|', 'Di Mola|Antonia|A|', 'Diehl|Bernd|B|']
D001897:Boronic Acids D002623:Chemistry Techniques, Analytical D004340:Drug Contamination D004952:Esters D009682:Magnetic Resonance Spectroscopy D010767:Phosphorylcholine D013237:Stereoisomerism
Chiral boronic acid Chiral derivatizing agent Enantiomeric excess determination NMR analysis