Utility of loop-mediated isothermal amplification as a point-of-care test in diagnosis of amoebic liver abscess.
Definitive diagnosis of amoebic liver abscess is challenging owing to the unavailability of sensitive commercial point-of-care molecular tests. The primary aim of our prospective diagnostic study was to compare available laboratory methods for the diagnosis of Entamoeba histolytica in clinical samples with loop-mediated isothermal amplification. We compared deoxyribonucleic acid (DNA) analysis methods, namely, loop-mediated isothermal amplification and reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) using pus, stool and blood samples from 200 patients with clinical and radiological diagnosis of amoebic liver abscess. Loop-mediated isothermal amplification had significantly higher sensitivity (88%) as compared to reverse transcriptase polymerase chain reaction (64%) and excellent specificity (100%).
['Handa|Dipti|D|', 'Gupta|Monica|M|https://orcid.org/0000-0002-3992-1013', 'Lehl|Sarabmeet Singh|SS|', 'Gupta|Amit|A|', 'Singh|Ram|R|']
D000913:Antibodies, Protozoan D016054:DNA, Protozoan D004748:Entamoeba histolytica D004797:Enzyme-Linked Immunosorbent Assay D006801:Humans D008101:Liver Abscess, Amebic D025202:Molecular Diagnostic Techniques D021141:Nucleic Acid Amplification Techniques D000067716:Point-of-Care Testing D016133:Polymerase Chain Reaction D011446:Prospective Studies D012680:Sensitivity and Specificity
loop-mediated isothermal amplification amoebic liver abscess Entamoeba histolytica reverse transcriptase polymerase chain reaction enzyme linked immunosorbent assay