Mercury detection based on label-free and isothermal enzyme-free amplified fluorescence platform.
A novel and convenient biosensor for Mercury (II) detection was developed based on toehold-mediated strand displacement isothermal enzyme-free amplification (EFA) technology and label-free fluorescence platform using Sybr Green Ι (SG) and graphene oxide (GO). The method is highly sensitive and selective, and the logarithmically related Hg2+ linearity range is from 0.1 to 50nM with a detection limit 0.091nM. Moreover, our strategy is simple, enzyme-free, and inexpensive and can be applied to detect spiked Hg2+ in environmental water samples with good recovery and accuracy, which demonstrates that the biosensor has a good potential in the environment surveys in the future.
['Meng|Fanbin|F|', 'Xu|Hui|H|', 'Yao|Xue|X|', 'Qin|Xuan|X|', 'Jiang|Tingting|T|', 'Gao|Shanmin|S|', 'Zhang|Yahui|Y|', 'Yang|Di|D|', 'Liu|Xia|X|']
Isothermal enzyme-free amplification Label-free fluorescence platform Hg(2+)