The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation.
We investigated the influence of the site of administration of DNA vaccine on the induced immune response. DNA vaccines were administered by electroporation at three different sites: tibial cranial muscle, abdominal skin and ear pinna. Aiming to draw general conclusions about DNA vaccine delivery, we successively used several plasmids encoding either luciferase and ovalbumin as models or gp160 and P1A as vaccines against HIV and P815 mastocytoma, respectively. Low levels and duration of luciferase transgene expression were observed after electroporation of the abdominal skin, partly explaining its lower immunogenic performance as compared to the other sites of administration. Analyses of OT-I CD8+ and OT-II CD4+ T cell responses highlighted the differential impact of the delivery site on the elicited immune response. Muscle electroporation induced the strongest humoral immune response and both muscle and ear pinna sites induced cellular immunity against gp160. Ear pinna delivery generated the highest level of CTL responses against P1A but electroporation of muscle and ear pinna were equally efficient in delaying P815 growth and improving mice survival. The present study demonstrated that the site of administration is a key factor to be tested in the development of DNA vaccine.
['Vandermeulen|Gaëlle|G|', 'Vanvarenberg|Kevin|K|', 'De Beuckelaer|Ans|A|', 'De Koker|Stefaan|S|', 'Lambricht|Laure|L|', 'Uyttenhove|Catherine|C|', 'Reschner|Anca|A|', 'Vanderplasschen|Alain|A|', 'Grooten|Johan|J|', 'Préat|Véronique|V|']
D016915:AIDS Vaccines D000005:Abdomen D000279:Administration, Cutaneous D000818:Animals D000951:Antigens, Neoplasm D015496:CD4-Positive T-Lymphocytes D018414:CD8-Positive T-Lymphocytes D019496:Cancer Vaccines D054644:Ear Auricle D018274:Electroporation D005260:Female D015483:HIV Antibodies D019302:HIV Envelope Protein gp160 D015497:HIV-1 D006614:Hindlimb D007111:Immunity, Cellular D056724:Immunity, Humoral D008156:Luciferases D008810:Mice, Inbred C57BL D018482:Muscle, Skeletal D010957:Plasmids D012867:Skin D014611:Vaccination D019444:Vaccines, DNA
DNA vaccine Electroporation Delivery site HIV vaccine Tumor vaccine