A rapid and enhanced DNA detection method for crop cultivar discrimination.
In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products.
['Monden|Yuki|Y|', 'Takasaki|Kazuto|K|', 'Futo|Satoshi|S|', 'Niwa|Kousuke|K|', 'Kawase|Mitsuo|M|', 'Akitake|Hiroto|H|', 'Tahara|Makoto|M|']
D000383:Agriculture D002845:Chromatography D018556:Crops, Agricultural D017931:DNA Primers D018744:DNA, Plant D004587:Electrophoresis, Agar Gel D031985:Fragaria D009693:Nucleic Acid Hybridization D012680:Sensitivity and Specificity D013045:Species Specificity
Cultivar discrimination Strawberry Retrotransposon Practical application Multiplex PCR