Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and Tissues.
Keratin filaments are a hallmark of epithelial differentiation. Their cell type-specific spatial organization and dynamic properties reflect and support epithelial function. To study this interdependency, imaging of fluorescently tagged keratins is a widely used method by which the temporospatial organization and behavior of the keratin intermediate filament network can be analyzed in living cells. Here, we describe methods that have been adapted and optimized to dissect and quantify keratin intermediate filament network dynamics in vital cultured cells and functional tissues.
['Schwarz|Nicole|N|', 'Moch|Marcin|M|', 'Windoffer|Reinhard|R|', 'Leube|Rudolf E|RE|']
D000818:Animals D003599:Cytoskeleton D036681:Fluorescence Recovery After Photobleaching D006801:Humans D007382:Intermediate Filaments D007633:Keratins D051379:Mice D018613:Microscopy, Confocal
Blastocyst Bulk flow analysis Confocal microscopy Cytokeratin Fluorescence recovery after photobleaching Fluorescent protein Live cell imaging Motion analysis Preimplantation embryo Transgenic mouse