The Zebrafish as a Model for Human Bacterial Infections.
The development of the zebrafish (Danio rerio) infectious disease model has provided new insights and information into pathogenesis. Many of these new discoveries would not have been possible using a typical mammalian model. The advantages of using this model are many and in the last 15 years the model has been exploited for the analysis of many different pathogens. Here, we describe in detail how to perform a bacterial infection using either the adult zebrafish or zebrafish larvae using microinjection. Multiple methods of analysis are described that can be used to address specific questions pertaining to disease progression and the interactions with the immune system.
['Neely|Melody N|MN|']
D000818:Animals D001424:Bacterial Infections D001706:Biopsy D004195:Disease Models, Animal D006801:Humans D007150:Immunohistochemistry D007814:Larva D008845:Microinjections D008856:Microscopy, Fluorescence D013290:Streptococcal Infections D015027:Zebrafish
Zebrafish Infection Pathogenesis Microinjection Animal model Histology Streptococcus infection