Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents.
While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value.
['Powell-Palm|Matthew J|MJ|', 'Zhang|Yanfang|Y|', 'Aruda|Justin|J|', 'Rubinsky|Boris|B|']
D000818:Animals D002470:Cell Survival D003080:Cold Temperature D015925:Cryopreservation D003451:Cryoprotective Agents D005615:Freezing D007515:Islets of Langerhans D023421:Models, Animal D009995:Osmosis D051381:Rats D017207:Rats, Sprague-Dawley D013816:Thermodynamics
Pancreatic islets Cryoprotective agents Isochoric cryopreservation Organ cryopreservation