Application of gas chromatography/flame ionization detector-based metabolite fingerprinting for authentication of Asian palm civet coffee (Kopi Luwak).
Development of authenticity screening for Asian palm civet coffee, the world-renowned priciest coffee, was previously reported using metabolite profiling through gas chromatography/mass spectrometry (GC/MS). However, a major drawback of this approach is the high cost of the instrument and maintenance. Therefore, an alternative method is needed for quality and authenticity evaluation of civet coffee. A rapid, reliable and cost-effective analysis employing a universal detector, GC coupled with flame ionization detector (FID), and metabolite fingerprinting has been established for discrimination analysis of 37 commercial and non-commercial coffee beans extracts. gas chromatography/flame ionization detector (GC/FID) provided higher sensitivity over a similar range of detected compounds than GC/MS. In combination with multivariate analysis, GC/FID could successfully reproduce quality prediction from GC/MS for differentiation of commercial civet coffee, regular coffee and coffee blend with 50 wt % civet coffee content without prior metabolite details. Our study demonstrated that GC/FID-based metabolite fingerprinting can be effectively actualized as an alternative method for coffee authenticity screening in industries.
['Jumhawan|Udi|U|', 'Putri|Sastia Prama|SP|', 'Yusianto|||', 'Bamba|Takeshi|T|', 'Fukusaki|Eiichiro|E|']
D000818:Animals D003069:Coffee D016002:Discriminant Analysis D005410:Flame Ionization D019649:Food Industry D008401:Gas Chromatography-Mass Spectrometry D055442:Metabolome D015999:Multivariate Analysis D012015:Reference Standards D045949:Viverridae
Metabolite fingerprinting Civet coffee Kopi Luwak Gas chromatography/flame ionization detector Industrial routine