A novel magnetic solid-phase extraction method for detection of 14 heterocyclic aromatic amines by UPLC-MS/MS in meat products.
The current study developed a cheap and effective method for the simultaneous extraction of 14 heterocyclic aromatic amines (HAAs) in food matrix. Core-shell Fe3O4@PDA nanoparticles were constructed and acted as the magnetic solid-phase extraction adsorbent to separate and purify HAAs from meat products for the first time. Then, UPLC-MS/MS technique was employed to identify and quantify the HAAs easily. Fe3O4@PDA nanoparticles were synthesized and characterized successfully. Totally 14 HAAs were completely separated in 19.99 min with good regression coefficients. LODs and LOQs were in the range of 0.013-0.247 ng/g and 0.056-0.803 ng/g, respectively. The intra-day precisions and inter-day precisions were below 9%. Except for IQ[4,5-b], Phe-p-1, PhIP, other 11 types of HAAs (DMIP, 1,5,6-TMIP, IQ, IQx, MeIQ, MeIQx, 7,8-DiMeIQx, AαC, MeAαC, Harman, Norharman) could acquire relatively high recoveries (71.06%-108.49%). The proposed method was successfully devoted to the evaluation of HAAs levels in 8 commercial meat products to verify the adaptability.
['Shan|Shihui|S|', 'Ma|Yunjiao|Y|', 'Sun|Changling|C|', 'Guo|Xiaolu|X|', 'Zheng|Han|H|', 'Xu|Xianbing|X|', 'Qin|Lei|L|', 'Hu|Jiangning|J|']
D000588:Amines D000818:Animals D002243:Carbolines D002853:Chromatography, Liquid D005504:Food Analysis D006571:Heterocyclic Compounds D007093:Imidazoles D007211:Indoles D060328:Magnetic Phenomena D008461:Meat Products D008855:Microscopy, Electron, Scanning D053758:Nanoparticles D011108:Polymers D000080305:Pork Meat D011804:Quinolines D011810:Quinoxalines D052616:Solid Phase Extraction D017550:Spectroscopy, Fourier Transform Infrared D053719:Tandem Mass Spectrometry
Heterocyclic aromatic amines UPLC-MS/MS Meat products Fe(3)O(4)@PDA Magnetic dispersive solid-phase extraction