Elevenin regulates the body color through a G protein-coupled receptor NlA42 in the brown planthopper Nilaparvata lugens.
The neuropeptide elevenin and similar neuropeptide precursors are common in some invertebrates but their physiological function in most species has not been explored. The brown planthopper, Nilaparvata lugens (Stål) has an elevenin-like peptide and a G protein-coupled receptor (GPCR) NlA42 that is homologous to the elevenin receptor of the annelid Platynereis dumerilii. RNA interference (RNAi)-mediated knockdown of either Nl-elevenin or the NlA42 gene resulted in cuticle melanization. Ion transport peptide (ITP) also induces melanization, but unlike ITP, knockdown of NlElevenin and NlA42 did not have any effect on wing expansion or activity after eclosion. In wild condition macropterous individuals show a darker body color when compared with brachypterous individuals, but RNAi experiments suggest that insulin-signaling and Nl-elevenin signaling regulate wing morph and body color independently. NlElevenin was predominantly expressed in the brain while NlA42 was highly expressed in the abdominal integument and brain. A signal Calcium assays using aequorin indicated that NlA42 heterologously expressed in HEK293 cells exhibited responses to synthetic Nl-elevenin peptide from concentrations as low as 10-9M. These results suggest that neuropeptide Nl-elevenin is involved in the regulation of melanization through its receptor NlA42. This is the first report of a physiological function for elevenin-like peptides in insects.
['Uchiyama|Hironobu|H|', 'Maehara|Shiori|S|', 'Ohta|Hiroto|H|', 'Seki|Takehito|T|', 'Tanaka|Yoshiaki|Y|']
D000818:Animals D020869:Gene Expression Profiling D005786:Gene Expression Regulation D057809:HEK293 Cells D006430:Hemiptera D006801:Humans D019476:Insect Proteins D009479:Neuropeptides D010858:Pigmentation D034622:RNA Interference D012333:RNA, Messenger D043562:Receptors, G-Protein-Coupled D048348:Reverse Transcription D013997:Time Factors
Neuropeptide RNA interference G-protein coupled receptor Insect hemiptera