Versatile Hyperbranched Poly(β-hydrazide ester) Macromers as Injectable Antioxidative Hydrogels.
Synthetic reactive oxygen species (ROS)-responsive biomaterials have emerged as a useful platform for regulating critical aspects of ROS-induced pathologies and can improve such hostile microenvironments. Here, we report a series of new hyperbranched poly(β-hydrazide ester) macromers (HB-PBHEs) with disulfide moieties synthesized via an "A2 + B4" Michael addition approach. The three-dimensional structure of HB-PBHEs with multiacrylate end groups endows the macromers with rapid gelation capabilities to form (1) injectable hydrogels via cross-linking with thiolated hyaluronic acid and (2) robust UV-cross-linked hydrogels. The disulfide-containing macromers and hydrogels exhibit H2O2-responsive degradation compared with the counterparts synthesized by a dihydrazide monomer without disulfide moieties. The cell viability under a high ROS environment can be well-maintained under the protection of the disulfide containing hydrogels.
['Xu|Qian|Q|', 'Venet|Manon|M|', 'Wang|Wei|W|', 'Creagh-Flynn|Jack|J|', 'Wang|Xi|X|', 'Li|Xiaolin|X|', 'Gao|Yongsheng|Y|http://orcid.org/0000-0002-2347-1855', 'Zhou|Dezhong|D|', 'Zeng|Ming|M|', 'Lara-Sáez|Irene|I|', 'A|Sigen|S|', 'Tai|Hongyun|H|', 'Wang|Wenxin|W|http://orcid.org/0000-0002-5053-0611']
D016475:3T3 Cells D017667:Adipocytes D000818:Animals D000975:Antioxidants D001386:Azides D001713:Biphenyl Compounds D002470:Cell Survival D018920:Coculture Techniques D004247:DNA D004220:Disulfides D004952:Esters D016166:Free Radical Scavengers D006801:Humans D020100:Hydrogels D009682:Magnetic Resonance Spectroscopy D051379:Mice D010851:Picrates D011487:Protein Conformation D017382:Reactive Oxygen Species D012212:Rheology D013056:Spectrophotometry, Ultraviolet D013234:Stem Cells D023822:Tissue Engineering
poly(β-hydrazide ester) injectable antioxidant hydrogel tissue engineering