Visual multiple recognition of protein biomarkers based on an array of aptamer modified gold nanoparticles in biocomputing to strip biosensor logic operations.
We developed a strip biosensors array based on aptamer-modified gold nanoparticles as receptors and combined the protein-aptamer binding reaction with the streptavidin-biotin interaction as well as the sandwich format. We found that a series of protein receptors obtained a distinct response pattern to each target protein. Three proteins have been well distinguished with the naked eyes and a portable reader without mutual interference, accompanying with lower limit of detection and wider linear range. A complete set of four elementary logic gates (AND, OR, INH, and NAND) and eight combinative logic gates (AND-OR; AND-INH; OR-INH; INH-NAND; AND-OR-INH; AND-INH-NAND; OR-INH-NAND; AND-OR-INH-NAND) are thoroughly realized using this array, which could eventually be applicable to the keypad-lock system with enhanced complexity in the near future. Moreover, this array shows excellent linear relationships, anti-interference capability, real human serum samples applicability, long-term storage stability and reproducibility. All indicate that this design has very good prospects for development.
['Qin|Chunyan|C|', 'Gao|Ya|Y|', 'Wen|Wei|W|', 'Zhang|Xiuhua|X|', 'Wang|Shengfu|S|']
D052157:Aptamers, Nucleotide D015415:Biomarkers D015374:Biosensing Techniques D001710:Biotin D039301:Computers, Molecular D006046:Gold D006801:Humans D053768:Metal Nanoparticles D011506:Proteins D015203:Reproducibility of Results D019809:Streptavidin
Protein Aptamers Gold nanoparticles Logic gate The strip biosensors array