Grafted muscle-derived stem cells promote the therapeutic efficiency of epimysium conduits in mice with peripheral nerve gap injury.
Our research aimed to build allogeneic artificial conduits with epimysium and muscle-derived stem cells (MDSCs) from the skeletal muscle of mice. We applied the conduit to repair peripheral nerve defects and estimated the effectiveness of the repair process. In the research, we prepared epimysium conduits with lumens to bridge repair a 5-mm-long sciatic nerve defect from C57 wild-type mice and then transplanted green fluorescent protein (GFP)-MDSCs and Matrigel suspensions into the conduit. Histological and functional assessments were performed 4 and 8 weeks after surgery. The tissue-engineered conduit from muscle effectively repaired the nerve defect, while the group with GFP-MDSCs showed improved histological examinations and functional assessments, and the newborn nerves highly expressed GFP. As the results suggested, autologous epimysium conduits represent a reliable method to repair peripheral nerve defects, and the addition of MDSCs promote the effectiveness of differentiating into multiple lineages. Our research simultaneously demonstrated the myogenic, neurogenic, and angiogenic potential of MDSCs in vivo for the first time.
['Xu|Zhuqiu|Z|https://orcid.org/0000-0002-6259-2668', 'Chen|Zixiang|Z|', 'Feng|Weifeng|W|', 'Huang|Minlu|M|', 'Yang|Xiaonan|X|https://orcid.org/0000-0002-3353-6982', 'Qi|Zuoliang|Z|']
D000818:Animals D005260:Female D008810:Mice, Inbred C57BL D018482:Muscle, Skeletal D059348:Peripheral Nerve Injuries D020127:Recovery of Function D000074586:Remyelination D033581:Stem Cell Transplantation D023822:Tissue Engineering
stem cells microsurgery peripheral nerve injury regenerative medicine tissue engineering