Comparative transcriptome analyses on terpenoids metabolism in field- and mountain-cultivated ginseng roots.
BACKGROUND
There exist differences in morphological traits and phytochemical compositions between field- and mountain-cultivated Panax ginseng (FCG and MCG), which might be attributed to variations of terpenoids metabolism adapting to different growth conditions. The present work aims to uncover these variations.


RESULTS
Among 26,648 differentially expressed genes, 496 genes distributed in seven dominant terpenoids pathways were identified. Diterpenoids and triterpenoids biosynthesis genes were significantly higher-expressed in FCG root. Conversely, biosynthesis of carotenoids was significantly more active in MCG root. Additionally, terpenoids backbones, monoterpenoids, sesquiterpenoids, and terpenoid-quinones biosyntheses were neither obviously inclined. Our determination also revealed that there were more gibberellins and steroids accumulated in FCG root which might be responsible for its quick vegetative growth, and enriched abscisic acid and germacrenes as well as protopanaxatriol-type ginsenosides might be major causes of enhanced stress-resistance in MCG root.


CONCLUSIONS
The study firstly provided an overview of terpenoids metabolism in roots of FCG and MCG in elucidating the underlying mechanisms for their different morphological appearances and phytochemical compositions.
['Fan|Hang|H|', 'Li|Ke|K|', 'Yao|Fan|F|', 'Sun|Liwei|L|', 'Liu|Yujun|Y|http://orcid.org/0000-0001-6588-3359']
D000069599:Crop Production D004224:Diterpenes D020869:Gene Expression Profiling D018506:Gene Expression Regulation, Plant D053858:Metabolic Networks and Pathways D005894:Panax D018517:Plant Roots D013729:Terpenes
Mountain-cultivated ginseng Ginsenosides Field-cultivated ginseng Terpenoid phytohormones Terpenoids biosynthesis genes Transcriptome analysis