CD4(+) CD25(high) forkhead box protein 3(+) regulatory T lymphocytes suppress interferon-γ and CD107 expression in CD4(+) and CD8(+) T cells from tuberculous pleural effusions.
Tuberculous pleural effusion is characterized by a T helper type 1 (Th1) profile, but an excessive Th1 response may also cause tissue damage that might be controlled by regulatory mechanisms. In the current study we investigated the role of regulatory T cells (Treg ) in the modulation of Th1 responses in patients with tuberculous (TB) pleurisy. Using flow cytometry we evaluated the proportion of Treg (CD4(+) CD25(high) forkhead box protein 3(+) ), interferon (IFN)-γ and interleukin (IL)-10 expression and CD107 degranulation in peripheral blood (PB) and pleural fluid (PF) from patients with TB pleurisy. We demonstrated that the proportion of CD4(+) CD25(+) , CD4(+) CD25(high) FoxP3(+) and CD8(+) CD25(+) cells were increased in PF compared to PB samples. Mycobacterium tuberculosis stimulation increased the proportion of CD4(+) CD25(low/neg) IL-10(+) in PB and CD4(+) CD25(low/neg) IFN-γ(+) in PF; meanwhile, CD25(high) mainly expressed IL-10 in both compartments. A high proportion of CD4(+) CD107(+) and CD8(+) CD107(+) cells was observed in PF. Treg depletion enhanced the in-vitro M. tuberculosis-induced IFN-γ and CD4(+) and CD8(+) degranulation responses and decreased CD4(+) IL-10(+) cells in PF. Our results demonstrated that in TB pleurisy Treg cells effectively inhibit not only IFN-γ expression but also the ability of CD4(+) and CD8(+) cells to degranulate in response to M. tuberculosis.
['Geffner|L|L|', 'Basile|J I|JI|', 'Yokobori|N|N|', 'Sabio Y García|C|C|', 'Musella|R|R|', 'Castagnino|J|J|', 'Sasiain|M C|MC|', 'de la Barrera|S|S|']
D000328:Adult D018414:CD8-Positive T-Lymphocytes D015550:Cell Degranulation D005260:Female D051858:Forkhead Transcription Factors D006801:Humans D007371:Interferon-gamma D016753:Interleukin-10 D053645:Interleukin-2 Receptor alpha Subunit D007963:Leukocytes, Mononuclear D052118:Lysosomal-Associated Membrane Protein 1 D008297:Male D009169:Mycobacterium tuberculosis D010996:Pleural Effusion D050378:T-Lymphocytes, Regulatory D014396:Tuberculosis, Pleural D055815:Young Adult
CD107 pleural effusions tuberculosis T regulatory