Evolution of microsatellite Loci of tropical and temperate anguilla eels.
Anguilla eels are divided into temperate and tropical eels, based on their major distributions. The present study collected two temperate eels, Anguilla japonica and Anguilla anguilla, and two tropical eels, Anguilla marmorata and Anguilla bicolor pacifica, to examine two questions: do temperate and tropical Anguilla eels have different genetic polymorphic patterns?; and do temperate Anguilla japonica and Anguilla anguilla have a closer relationship to each other than to tropical eels? In total, 274 sequences were cloned and sequenced from six conserved microsatellite loci to examine polymorphic patterns of these four catadromous eels. Different mutational events, including substitutions, and repeat-unit deletions and insertions, appeared in major regions, while different point mutations were observed in flanking regions. The results implied that parallel patterns of microsatellite sequences occurred within both tropical and temperate freshwater eels. Consensus flanking sequences of six homologous loci from each of the four species were constructed. Genetic distances ranged from 0.044 (Anguilla bicolor pacifica vs. Anguilla marmorata) to 0.061 (Anguilla marmorata vs. Anguilla anguilla). The tree topology suggests the hypothesis of Anguilla japonica and Anguilla anguilla being a sister group must be rejected.
['Tseng|Mei-Chen|MC|']
D000811:Anguilla D000818:Animals D001483:Base Sequence D005075:Biological Evolution D016384:Consensus Sequence D019143:Evolution, Molecular D049810:Genetic Speciation D018895:Microsatellite Repeats D008969:Molecular Sequence Data D009154:Mutation D010802:Phylogeny D011110:Polymorphism, Genetic D016415:Sequence Alignment D017422:Sequence Analysis, DNA
polymorphic pattern major region flanking sequence