Recommendations for Good Practice in Mass Spectrometry-Based Lipidomics.
In the last two decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards Initiative (LSI) is a community-based endeavor for the coordination of development of these best practice guidelines in lipidomics, and is embedded within the International Lipidomics Society (ILS). It is the intention of this review to highlight the most quality-relevant aspects of the lipidomics workflow, including preanalytics, sample preparation, mass spectrometry, and lipid species identification and quantitation. Furthermore, this review does not just highlight examples of best practice, but also sheds light on strengths, drawbacks, and pitfalls in the lipidomic analysis workflow. While this review is not designed to be a step-by-step protocol by itself, nor to be dedicated to a specific application of lipidomics, it should nevertheless provide the interested reader with links and original publications to obtain a comprehensive overview concerning the state of the art practices in the field.
['Köfeler|Harald C|HC|', 'Ahrends|Robert|R|', 'Baker|Erin S|ES|', 'Ekroos|Kim|K|', 'Han|Xianlin|X|', 'Hoffmann|Nils|N|', 'Holčapek|Michal|M|', 'Wenk|Markus R|MR|', 'Liebisch|Gerhard|G|']
Lipidomics Mass Spectrometry Chromatography Ion Mobility Spectrometry LC-MS Lipid Identification Metabolomics Phospholipids Sphingolipids