Daphne mucronata enhances cell proliferation and protects human adipose stem cells against monosodium iodoacetate induced oxidative stress in vitro.
Mesenchymal stem cells (MSCs) are being used to treat many diseases as they exhibit great regenerative potential. However, MSC's transplantation sometimes does not yield the maximum regenerative outcome as they are unable to survive in inflammatory conditions. Several approaches including preconditioning are used to improve the survival rate of mesenchymal stem cells. One such recently reported approach is preconditioning MSCs with plant extracts. The present study was designed to evaluate the effect of Daphne mucronata extract on stressed human adipose-derived mesenchymal stem cells (hADMSCs). Isolated hADMSCs were preconditioned with different concentrations of Daphne muconata extract and the protective, proliferative, antioxidant and anti-inflammatory effect was assessed through various assays and expression analysis of inflammatory markers regulated through NF-κB pathway. Results suggest that preconditioning hADMSCs with Daphne mucronata increased the cell viability, proliferative and protective potential of hADMSCs with a concomitant reduction in LDH, ROS and elevation in SOD activity. Moreover, both the ELISA and gene expression analysis demonstrated down regulations of inflammatory markers (IL1-β, TNF-α, p65, p50, MMP13) in Daphne mucronata preconditioned hADMSCs as compared to stress. This is the first study to report the use of MIA induced oxidative stress against hADMSC's and effect of Daphne mucronata on stressed hADMSCs. Results of these studies provided evidence that Daphne mucronata protects the hADMSCs during stress conditions by down regulating the inflammatory markers and hence increase the viability and proliferative potential of hADMSCs that is crucial for transplantation purposes.
['Fazal|Numan|N|0000-0003-4407-9139', 'Khawaja|Hamzah|H|', 'Naseer|Nadia|N|', 'Khan|Azim Jahangir|AJ|', 'Latief|Noreen|N|']
D000273:Adipose Tissue D000975:Antioxidants D015415:Biomarkers D002465:Cell Movement D049109:Cell Proliferation D002470:Cell Survival D002478:Cells, Cultured D016207:Cytokines D019610:Cytoprotection D032364:Daphne D006801:Humans D016130:Immunophenotyping D018836:Inflammation Mediators D007461:Iodoacetates D059630:Mesenchymal Stem Cells D018384:Oxidative Stress D064209:Phytochemicals D010936:Plant Extracts
Daphne mucronata proliferation monosodium iodoacetate Mesenchymal stem cells preconditioning cell viability