Inhibitory Effects on NO Production and DPPH Radicals and NBT Superoxide Activities of Diarylheptanoid Isolated from Enzymatically Hydrolyzed Ehthanolic Extract of Alnus sibirica.
Alnus sibirica (AS) is geographically distributed in Korea, Japan, Northeast China, and Russia. Various anti-oxidant, anti-inflammation, anti-atopic dermatitis and anti-cancer biological effects of AS have been reported. Enzymatic hydrolysis decomposes the sugar bond attached to glycoside into aglycone which, generally, has a superior biological activity, compared to glycoside. Enzymatic hydrolysis of the extract (EAS) from AS was processed and the isolated compounds were investigated-hirsutanonol (1), hirsutenone (2), rubranol (3), and muricarpon B (4). The structures of these compounds were elucidated, and the biological activities were assessed. The ability of EAS and the compounds (1-4) to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and Nitroblue tetrazolium (NBT) superoxide, and to inhibit NO production was evaluated in vitro. EAS showed more potent antioxidant and anti-inflammatory activity than AS. All investigated compounds showed excellent antioxidant and anti-inflammatory activities.
['Wang|Hye Soo|HS|', 'Hwang|Yoon Jeong|YJ|', 'Yin|Jun|J|', 'Lee|Min Won|MW|']
D029661:Alnus D000818:Animals D001713:Biphenyl Compounds D036381:Diarylheptanoids D000431:Ethanol D006868:Hydrolysis D020128:Inhibitory Concentration 50 D051379:Mice D009569:Nitric Oxide D009580:Nitroblue Tetrazolium D010851:Picrates D010936:Plant Extracts D000067996:RAW 264.7 Cells D013481:Superoxides
Alnus sibirica enzymatic hydrolysis antioxidant anti-inflammatory hirsutanonol oregonin