Colorimetric/fluorogenic detection of thiols by N-fused porphyrin in water.
A water-soluble derivative of N-fused porphyrin (NFP) possessing four cationic side-arms (pPyNFP) serves as a unique class of colorimetric/fluorogenic reporters that selectively react with biothiols in aquaous media to afford N-confused porphyrin (NCP) derivatives, while other nucleophilic amino acids were inert under a wide range of pH conditions. Owing to the large difference of the optical properties between NCP and NFP, the transformation enabled selective detection of biothiols in colorimetric/fluorogenic manner, especially in the near-infrared region. To the best our knowledge, this is the first example of porphyrin-based thiol detection systems that use the direct attack of thiol group on the optical reporter.
['Ikawa|Yoshiya|Y|', 'Touden|Satoshi|S|', 'Katsumata|Sho|S|', 'Furuta|Hiroyuki|H|']
D005456:Fluorescent Dyes D006863:Hydrogen-Ion Concentration D011166:Porphyrins D013050:Spectrometry, Fluorescence D013438:Sulfhydryl Compounds D014867:Water
N-fused porphyrin Biothiol N-confused porphyrin Near-infrared Cysteine Glutathione Homocysteine