Platelet-derived growth factor BB stimulates differentiation of rat immature Leydig cells.
Platelet-derived growth factor (PDGF) is one family of growth factors that regulate cell growth and differentiation. Rat Leydig cells express PDGF-β receptor (PDGFRB) during pubertal development. However, the mechanism of PDGF in the regulation of Leydig cell development is unclear. In the present study, rat immature Leydig cells were isolated from the testes of 35-day-old Sprague-Dawley rats and treated with 1 and 10 ng/mL of PDGF-BB. After 24 h of treatment, these cells were harvested for genomics profiling and the medium steroids were measured. 1 and 10 ng/mL PDGF-BB significantly increased androgen production by rat immature Leydig cells. Genomics profiling analysis showed that the expression levels of steroidogenic acute regulatory protein (Star) were increased by 2-fold. Further analysis showed that Fos expression level was increased 2- and 5-fold by 1 and 10 ng/mL PDGF-BB, respectively. In conclusion, PDGF-BB stimulated the differentiation of rat immature Leydig cells via regulating Star.
['Wang|Yiyan|Y|', 'Li|Xiaoheng|X|', 'Ge|Fei|F|', 'Yuan|Kaiming|K|', 'Su|Zhijian|Z|', 'Wang|Guimin|G|', 'Lian|Qingquan|Q|', 'Ge|Ren-Shan|RS|']
D000728:Androgens D000818:Animals D000077214:Becaplermin D053898:Biosynthetic Pathways D002454:Cell Differentiation D002784:Cholesterol D004247:DNA D005786:Gene Expression Regulation D007985:Leydig Cells D008297:Male D020411:Oligonucleotide Array Sequence Analysis D020574:Proto-Oncogene Proteins c-sis D017207:Rats, Sprague-Dawley D060888:Real-Time Polymerase Chain Reaction D015203:Reproducibility of Results
Platelet-derived growth factor BB rat immature Leydig cells steroidogenic acute regulatory protein Fos