Heart-After-Liver Transplantation Attenuates Rejection of Cardiac Allografts in Sensitized Patients.
BACKGROUND
In patients undergoing heart transplantation, significant allosensitization limits access to organs, resulting in longer wait times and high waitlist mortality. Current desensitization strategies are limited in enabling successful transplantation.


OBJECTIVES
The purpose of this study was to describe the cumulative experience of combined heart-liver transplantation using a novel heart-after-liver transplant (HALT) protocol resulting in profound immunologic protection.


METHODS
Reported are the results of a clinical protocol that was instituted to transplant highly sensitized patients requiring combined heart and liver transplantation at a single institution. Patients were dual-organ listed with perceived elevated risk of rejection or markedly prolonged waitlist time due to high levels of allo-antibodies. Detailed immunological data and long-term patient and graft outcomes were obtained.


RESULTS
A total of 7 patients (age 43 ± 7 years, 86% women) with high allosensitization (median calculated panel reactive antibody = 77%) underwent HALT. All had significant, unacceptable donor specific antibodies (DSA) (>4,000 mean fluorescence antibody). Prospective pre-operative flow cytometric T-cell crossmatch was positive in all, and B-cell crossmatch was positive in 5 of 7. After HALT, retrospective crossmatch (B- and T-cell) became negative in all. DSA fell dramatically; at last follow-up, all pre-formed or de novo DSA levels were insignificant at <2,000 mean fluorescence antibody. No patients experienced >1R rejection over a median follow-up of 48 months (interquartile range: 25 to 68 months). There was 1 death due to metastatic cancer and no significant graft dysfunction.


CONCLUSIONS
A heart-after-liver transplantation protocol enables successful transplantation via near-elimination of DSA and is effective in preventing adverse immunological outcomes in highly sensitized patients listed for combined heart-liver transplantation.
['Daly|Richard C|RC|', 'Rosenbaum|Andrew N|AN|', 'Dearani|Joseph A|JA|', 'Clavell|Alfredo L|AL|', 'Pereira|Naveen L|NL|', 'Boilson|Barry A|BA|', 'Frantz|Robert P|RP|', 'Behfar|Atta|A|', 'Dunlay|Shannon M|SM|', 'Rodeheffer|Richard J|RJ|', 'Schirger|John A|JA|', 'Taner|Timucin|T|', 'Gandhi|Manish J|MJ|', 'Heimbach|Julie K|JK|', 'Rosen|Charles B|CB|', 'Edwards|Brooks S|BS|', 'Kushwaha|Sudhir S|SS|']
D000328:Adult D002985:Clinical Protocols D015331:Cohort Studies D005260:Female D006084:Graft Rejection D016027:Heart Transplantation D006801:Humans D016031:Liver Transplantation D008297:Male D008875:Middle Aged D014181:Transplantation Immunology
rejection sensitization heart transplantation combined organ transplantation