Polymeric nanoassemblies entrapping curcumin overcome multidrug resistance in ovarian cancer.
The increasing emergence of multidrug-resistant (MDR) cells presents a challenge to effective cancer therapy. Curcumin (CUR) has multifunctional anticancer properties, but its clinical use has been limited by poor solubility. We developed biodegradable polymeric micelles entrapping CUR in order to improve its antitumor activity and to explore whether it could treat MDR cells. This delivery system produced small micelles with a high encapsulation efficiency, good stability, and slow release of CUR. CUR micelles showed cytotoxic effects in wild-type drug-sensitive A2780s and in paclitaxel-resistant A2780t ovarian adenocarcinoma cells. The concentration of free CUR that reduced cell viability by 50% (IC50) was 1.5 fold and 1.2 fold higher than that of CUR micelles in A2780s and A2780t cells, respectively. Cellular uptake studies indicated that delivery by micelles improved CUR uptake into both cell lines. Cell cycle analysis suggested that CUR micelles induced apoptosis and enhanced G2/M arrest. Overall, CUR micelles may provide a novel strategy to improve the clinical management of MDR ovarian cancer.
['Gou|Qiheng|Q|', 'Liu|Lei|L|', 'Wang|Chunting|C|', 'Wu|Qinjie|Q|', 'Sun|Lu|L|', 'Yang|Xi|X|', 'Xie|Yuxin|Y|', 'Li|Ping|P|', 'Gong|Changyang|C|']
D000970:Antineoplastic Agents D045744:Cell Line, Tumor D002470:Cell Survival D003474:Curcumin D018432:Drug Resistance, Multiple D019008:Drug Resistance, Neoplasm D004354:Drug Screening Assays, Antitumor D005260:Female D006801:Humans D008823:Micelles D010051:Ovarian Neoplasms D010316:Particle Size D013499:Surface Properties
Curcumin Multidrug resistant Ovarian cancer Biodegradable Micelles MPEG-PCL copolymer