Comprehensive metabonomic analysis of heart tissue from isoproterenol-induced myocardial infarction rat based on reversed-phase and hydrophilic interaction chromatography coupled to mass spectrometry.
We aim to describe the metabonomic characteristics of myocardial infarction rats. High-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was utilized to develop a metabonomic method of the heart homogenates of myocardial infarction rats. Hydrophilic interaction chromatography allows the analysis of high polar metabolites, providing complementary information to reversed-phase liquid chromatography. We combined reversed phase and hydrophilic interaction chromatographic separations to analyze 18 samples, ten from myocardial infarction rat hearts and eight from normal rat hearts. A total of 16 potential biomarkers in rat heart tissue were screened out, primarily related to oxidative stress, nitric oxide damage, taurine, and hypotaurine metabolism and sphingolipid metabolism. This research showed that a comprehensive metabonomic study is a useful tool to reveal the underlying mechanism of myocardial infarction.
['Sun|Lei|L|', 'Liu|Jianxun|J|http://orcid.org/0000-0003-4028-8427', 'Sun|Mingqian|M|', 'Lin|Li|L|', 'Miao|Lan|L|', 'Ge|Zhengyan|Z|', 'Yang|Bin|B|']
D000818:Animals D002851:Chromatography, High Pressure Liquid D006321:Heart D057927:Hydrophobic and Hydrophilic Interactions D007545:Isoproterenol D013058:Mass Spectrometry D055432:Metabolomics D009203:Myocardial Infarction D009206:Myocardium D051381:Rats
metabonomics myocardial infarction hydrophilic interaction chromatography biomarkers reversed-phase chromatography