Phenogenotypic Characterization of Escherichia coli O157:H7 Strains Isolated from Cattle at Slaughter.
Shiga toxin-producing Escherichia coli (STEC) O157:H7 was isolated from 30 (4%) of 744 cattle hide swab samples collected at Estonian slaughterhouses within a 3-year monitoring program of zoonotic pathogens. The isolates were characterized by determining the presence of STEC main virulence factors, the antimicrobial resistance profiles, and the genetic relatedness by pulsed-field gel electrophoresis (PFGE). Thirteen strains carried the stx2 gene alone and 17 both the stx1 and stx2 genes. The most frequently detected stx subtype was stx2c, occurring alone (n = 12) or in combination with subtype stx1a (n = 13). All isolates harbored the intimin-coding eae gene and produced enterohemolysin. Twelve isolates (40%) showed resistance to at least one of the 14 antimicrobials and the isolates were predominantly resistant to streptomycin, sulfamethoxazole, and ampicillin. No extended-spectrum beta-lactamase-producing isolates were detected. PFGE characterization of the isolates showed an overall similarity higher than 75%, and four clusters based on 100% similarity were revealed.
['Kramarenko|Toomas|T|', 'Roasto|Mati|M|', 'Mäesaar|Mihkel|M|', 'Maugliani|Antonella|A|', 'Tozzoli|Rosangela|R|', 'Meremäe|Kadrin|K|', 'Elias|Terje|T|', 'Kuningas|Maiu|M|']
D000003:Abattoirs D000818:Animals D000900:Anti-Bacterial Agents D002417:Cattle D002418:Cattle Diseases D024881:Drug Resistance, Bacterial D004927:Escherichia coli Infections D019453:Escherichia coli O157 D015964:Gene Expression Regulation, Bacterial D012307:Risk Factors D022603:Shiga Toxins D012867:Skin D015047:Zoonoses
Escherichia coli O157:H7 cattle slaughterhouse zoonotic agents