E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity.
Transendothelial migration (TEM) of neutrophils under blood flow is critical in the inflammatory cascade. However, the role of endothelial plasticity in this process is not fully understood. Therefore, we used an in vitro model to test the dynamics of human polymorphonuclear neutrophil (PMN) TEM across lipopolysaccharide-treated human umbilical vein endothelial cell (HUVEC) monolayers. Interestingly, shRNA-E-selectin knockdown in HUVECs destabilized endothelial junctional integrity by reducing actin branching and increasing stress fiber at cell-cell junctions. This process is accomplished by downregulating the activation of cortactin and Arp2/3, which in turn alters the adhesive function of VE-cadherin, enhancing PMN transmigration. Meanwhile, redundant P-selectins possess overlapping functions in E-selectin-mediated neutrophil adhesion, and transmigration. These results demonstrate, to our knowledge, for the first time, that E-selectins negatively regulate neutrophil transmigration through alterations in endothelial plasticity. Furthermore, it improves our understanding of the mechanisms underlying actin remodeling, and junctional integrity, in endothelial cells mediating leukocyte TEM.
['Huang|Dandan|D|', 'Ding|Qihan|Q|', 'Chen|Shenbao|S|', 'Lü|Shouqin|S|', 'Zhang|Yan|Y|', 'Long|Mian|M|']
D051377:Actin-Related Protein 2 D051378:Actin-Related Protein 3 D002465:Cell Movement D002478:Cells, Cultured D019040:E-Selectin D004730:Endothelium, Vascular D006801:Humans D007365:Intercellular Junctions D009504:Neutrophils D011554:Pseudopodia D057705:Transendothelial and Transepithelial Migration
E-selectin neutrophil transmigration cytoskeletal remodeling endothelial integrity