Development of a Multiplex PCR for Discrimination of the TLC:RS1:CTX array of Vibrio cholerae Wave 3 El Tor Strains.
Vibrio cholerae O1 serogroup Wave 3 El Tor strains are presently prevalent worldwide. The Wave 3 El Tor strains contain a TLC:RS1:CTX array on chromosome 1, and no element is integrated on chromosome 2. A multiplex PCR optimized to identify the TLC:RS1:CTX array of Wave 3 strains has been developed in this study. By using eight primers, the multiplex PCR can identify the characteristic CTX and RS1 array of Wave 3 strains from various arrays of strains belonging to other Waves. The four amplified DNA fragments of Wave 3 strains have been cloned in a vector, which could be used as a positive control for the multiplex PCR. This multiplex PCR and the positive control set could be useful tools for rapid recognition of Wave 3 El Tor strains.
['Kim|Eun Jin|EJ|', 'Yu|Hyun Jin|HJ|', 'Nair|G Balakrish|GB|', 'Kim|Dong Wook|DW|']
D002771:Cholera D002772:Cholera Toxin D002876:Chromosomes, Bacterial D017931:DNA Primers D006801:Humans D060885:Multiplex Polymerase Chain Reaction D014734:Vibrio cholerae
multiplex PCR V. cholerae