Influence of tacrolimus on podocyte injury inducted by angiotensin II.
OBJECTIVE
This study investigated the protective effect of FK506 in podocytes and the correlation of TRPC6 with podocyte injury repair.


METHODS
MPC5 were cultured in vitro, parallel with the control group, an Ang II stimulation group, and an FK506 intervention group. The apoptosis rate with flow cytometry also detected TRPC6 mRNA and protein expression by RT-PCR and Western blot, and then observed the distribution of TRPC6 with indirect immunofluorescence labeling under confocal microscope.


RESULTS
The activities of podocytes significantly increased after FK506 intervention for 24 hours and 48 hours when compared with the Ang II stimulation group, and the apoptosis rate was markedly lower than that of the Ang II stimulation group, while in contrast to TRPC6 mRNA and protein expression.


CONCLUSION
FK506 can directly act on the podocytes to inhibit Ang II-induced damage on podocyte structures and reduce the apoptosis rate of podocytes, which may be related to stabilizing TRPC6 expression and distribution in podocytes by FK506, thus maintaining the structure and function integrity of the slit diaphragm and playing a role in protecting podocytes and antiproteinuria.
['Shengyou|Yu|Y|', 'Li|Yu|Y|', 'Zhihong|Hao|H|', 'Yuanyuan|Ma|M|']
D000804:Angiotensin II D000818:Animals D017209:Apoptosis D015153:Blotting, Western D002460:Cell Line D048430:Cell Shape D005434:Flow Cytometry D005786:Gene Expression Regulation D051379:Mice D050199:Podocytes D011692:Puromycin Aminonucleoside D012333:RNA, Messenger D050052:TRPC Cation Channels D000074287:TRPC6 Cation Channel D016559:Tacrolimus D013997:Time Factors
Tacrolimus podocyte TRPC6 apoptosis proteinuria