A rapid qPCR method for genetic sex identification of Salmo salar and Salmo trutta including simultaneous elucidation of interspecies hybrid paternity by high-resolution melt analysis.
This study presents an improved duplex quantitative polymerase chain reaction (qPCR) method using the master sex-determining gene sdY as a marker for simultaneous genetic sex identification of salmonids of the Salmo genus and paternity elucidation for Salmo salar × Salmo trutta hybrids. This method will provide a new, simple and economical molecular tool for ecological studies of these species as well as for aquaculture purposes.
["Anglès d'Auriac|M B|MB|", 'Urke|H A|HA|', 'Kristensen|T|T|']
D000818:Animals D001483:Base Sequence D002678:Chimera D005260:Female D005819:Genetic Markers D008297:Male D008969:Molecular Sequence Data D016133:Polymerase Chain Reaction D020641:Polymorphism, Single Nucleotide D012487:Salmonidae D016415:Sequence Alignment D017422:Sequence Analysis, DNA D012732:Sex Determination Analysis
aquaculture fish ecology molecular diagnostic molecular sexing sdY gene species substitution