Proteomic Analysis of Rice Seedlings Under Cold Stress.
Low temperature can greatly restrict the growth and development of rice. The rice seedlings show growth retardation, lamina wrap, and part of blade even died under the condition of low temperature. In order to get more information about cold stress responses in rice, two dimensional electrophoresis and bioinformatics analysis of mass spectrometry were used to preliminary survey the cold tolerance of cold sensitive line 9311 and cold resistance variety Fujisaka 5 under cold stress. Two dimensional electrophoresis maps of 9311 and Fujisaka 5 were established under cold treatment. With analysis of bioinformation, the proteins were found involve in many aspects of rice development. The largest category of proteins is functioning on metabolism. By comparing the proteins from the two varieties, it can be found that most proteins from 9311 were down-regulated and were up-regulated in Fujisaka 5. The results showed that the membrane composition and structure were damaged, metabolism changed dramatically and rice defense system was activated under the cold stimulation. Fifty-nine proteins related to the resistance of cold stress were identified in our study, and we have investigated and classified all of their biological functions. The importance of our study are providing some conduct for the research of rice resistant to cold stress, supporting auxiliary technique for rice varieties and widening the search field of cold tolerance in plants.
['Ji|Li|L|', 'Zhou|Ping|P|', 'Zhu|Ya|Y|', 'Liu|Fang|F|', 'Li|Rongbai|R|', 'Qiu|Yongfu|Y|']
D003080:Cold Temperature D015180:Electrophoresis, Gel, Two-Dimensional D018507:Gene Expression Regulation, Developmental D018506:Gene Expression Regulation, Plant D063990:Gene Ontology D058977:Molecular Sequence Annotation D012275:Oryza D010940:Plant Proteins D030821:Plants, Genetically Modified D020543:Proteome D040901:Proteomics D036226:Seedlings D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization D013312:Stress, Physiological
Low temperature Mass spectrometry (MS) Rice (Oryza sativa L.) Two dimensional electrophoresis (2-DE)