Effect of Curcumin on Systemic T Helper 17 Cell Response; Gingival Expressions of Interleukin-17 and Retinoic Acid Receptor-Related Orphan Receptor γt; and Alveolar Bone Loss in Experimental Periodontitis.
BACKGROUND
Curcumin has anti-inflammatory and antioxidant effects and is reported to have many biologic activities. The current study examines effect of curcumin on: 1) systemic T helper 17 (Th17) cell response; 2) gingival expressions of interleukin (IL)-17 and retinoic acid receptor-related orphan receptor (ROR) γt; and 3) alveolar bone loss (ABL) in experimental periodontitis.


METHODS
Thirty-eight male albino Wistar rats were divided into four groups: 1) group 1 = periodontitis; 2) group 2 = periodontitis with curcumin treatment; 3) group 3 = periodontally healthy with curcumin treatment; and 4) group 4 = periodontally healthy. Curcumin was administered via oral gavage (30 mg/kg/d) for 15 days. After sacrifice via exsanguination, the following serum levels were determined using enzyme-linked immunosorbent assay: 1) IL-1β; 2) IL-6; 3) IL-17A; 4) IL-23; and 5) transforming growth factor- β. Morphometric evaluation of ABL was conducted and expression levels of IL-17 and RORγt in gingival tissues were evaluated immunohistochemically.


RESULTS
Group 2 had significantly lower ABL than group 1 (P <0.0125). Highest expression levels of IL-17 and RORγt were observed in group 1 and were significantly higher than those in all other groups (P <0.0125). The only serum biochemical parameter significantly different among groups was level of IL-23 (P <0.05). Serum IL-23 levels were higher in groups 1 and 2 than groups 3 and 4 (P <0.0125); however, they were not significantly different for groups 1 and 2 (P >0.0125).


CONCLUSION
Curcumin seems to be a promising host modulatory agent in periodontal disease pathogenesis regarding IL-17/IL-23 axis, with a decreasing effect on ABL and gingival expressions of IL-17 and RORγt.
['Bakır|Burcu|B|', 'Yetkin Ay|Zuhal|Z|', 'Büyükbayram|H İbrahim|Hİ|', 'Kumbul Doğuç|Duygu|D|', 'Bayram|Dilek|D|', 'Candan|I Aydın|IA|', 'Uskun|Ersin|E|']
D016301:Alveolar Bone Loss D000818:Animals D003474:Curcumin D020381:Interleukin-17 D008297:Male D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 D010518:Periodontitis D051381:Rats D017208:Rats, Wistar D018168:Receptors, Retinoic Acid
curcumin interleukin-17 Alveolar bone loss periodontitis Th17 interleukin-23