Dengue Virus Isolation in Mosquito Aedes albopictus Captured During an Outbreak in Tokyo, 2014, by a Method Relying on Antibody-Dependent Enhancement Mechanism Using FcγR-Expressing BHK Cells.
Dengue virus (DENV) isolation from mosquitoes is necessary for providing definitive evidence of virus circulation, and is critical for further virological characterization and determination of epidemiological characteristics. By using Aedes albopictus mosquitoes captured during an outbreak in Tokyo in 2014, we compared the DENV isolation rates of a conventional virus isolation method that uses C6/36 mosquito cells as assay cells with those of a virus isolation method that relies on an antibody-dependent enhancement (ADE) mechanism by using FcγR-expressing baby hamster kidney (BHK) cells and an antibody with ADE activity. The number of DENV genome copies and infectious virus titers in cell culture supernatant fluids of FcγR-expressing BHK cells were significantly higher than those of the C6/36 cells. In addition, DENV was isolated from a mosquito pool by using FcγR-expressing BHK cells only in the presence of infection-enhancing antibody. Infectious virus was detected in six mosquito pools only by using FcγR-expressing BHK cells. The results suggest that the method that relies on ADE mechanism by using the FcγR-expressing BHK cells and an antibody with ADE activity is useful for DENV isolation from mosquitoes caught in the field.
['Moi|Meng Ling|ML|', 'Kobayashi|Daisuke|D|', 'Isawa|Haruhiko|H|', 'Sasaki|Toshinori|T|', 'Saijo|Masayuki|M|', 'Kurane|Ichiro|I|', 'Sawabe|Kyoko|K|', 'Takasaki|Tomohiko|T|']
D000330:Aedes D000818:Animals D000914:Antibodies, Viral D002460:Cell Line D006224:Cricetinae D003715:Dengue D003716:Dengue Virus D016679:Genome, Viral D006801:Humans D000072138:Mosquito Vectors D017452:Receptors, IgG
dengue virus virus isolation mosquito Aedes albopictus antibody-dependent enhancement FcγR-expressing cells