Rapid detection of unconjugated estriol in the serum via superparamagnetic lateral flow immunochromatographic assay.
Unconjugated estriol (uE3) is one of the main naturally occurring estrogens that plays an important role in growth and development of the fetus. Usually, the level of uE3 is very low in men and non-pregnant women, but in pregnant women, the level of estriol has been found to be quite high. Therefore, the combination of uE3, AFP, and hCG is now widely used for Down Syndrome screening as a triple marker. Here, we developed a superparamagnetic lateral flow immunochromatographic assay to quantitatively detect uE3. The detection limit of this assay was 0.86 nmol/L and the linear range for the determination of uE3 was from 1 to 100 nmol/L. The detection time was 15 min and the assay had very low cross-reactivity with estrone (E1), estradiol (E2), and progesterone. The coefficient of variation (CV) of intra- and inter-assay ranged from 5% to 13%. The magnetic signals were stable under 37 °C within 7 d. Moreover, the concentrations of uE3 measured by lateral flow immunochromatographic assay in 230 serum samples collected from pregnant women at the Chinese People's Liberation Army General Hospital had a good correlation with those measured by time-resolved fluorescence immunoassay (R = 0.946).
['Wang|Ce|C|', 'Guan|Di|D|', 'Chen|Chen|C|', 'He|Shang|S|', 'Liu|Xiaoting|X|', 'Wang|Chengbin|C|', 'Wu|Huijuan|H|']
D015415:Biomarkers D002846:Chromatography, Affinity D004314:Down Syndrome D004964:Estriol D005260:Female D006801:Humans D057230:Limit of Detection D058185:Magnetite Nanoparticles D011247:Pregnancy D011296:Prenatal Diagnosis
Unconjugated estriol Lateral flow immunochromatographic assay Quantitative detection Post of care test (POCT) Superparamagnetic particles