Monoclonal antibodies raised against 167-180 aa sequence of human carbonic anhydrase XII inhibit its enzymatic activity.
Abstract Human carbonic anhydrase XII (CA XII) is a single-pass transmembrane protein with an extracellular catalytic domain. This enzyme is being recognized as a potential biomarker for different tumours. The current study was aimed to generate monoclonal antibodies (MAbs) neutralizing the enzymatic activity of CA XII. Bioinformatics analysis of CA XII structure revealed surface-exposed sequences located in a proximity of its catalytic centre. Two MAbs against the selected antigenic peptide spanning 167-180 aa sequence of CA XII were generated. The MAbs were reactive with recombinant catalytic domain of CA XII expressed either in E. coli or mammalian cells. Inhibitory activity of the MAbs was demonstrated by a stopped flow CO2 hydration assay. The study provides new data on the surface-exposed linear CA XII epitope that may serve as a target for inhibitory antibodies with a potential immunotherapeutic application.
['Dekaminaviciute|Dovile|D|', 'Kairys|Visvaldas|V|', 'Zilnyte|Milda|M|', 'Petrikaite|Vilma|V|', 'Jogaite|Vaida|V|', 'Matuliene|Jurgita|J|', 'Gudleviciene|Zivile|Z|', 'Vullo|Daniela|D|', 'Supuran|Claudiu T|CT|', 'Zvirbliene|Aurelija|A|']
D000595:Amino Acid Sequence D000818:Animals D000911:Antibodies, Monoclonal D002257:Carbonic Anhydrase Inhibitors D002256:Carbonic Anhydrases D019295:Computational Biology D004797:Enzyme-Linked Immunosorbent Assay D000939:Epitopes D004926:Escherichia coli D005260:Female D015870:Gene Expression D006433:Hemocyanins D006801:Humans D006825:Hybridomas D007114:Immunization D018796:Immunoconjugates D051379:Mice D008807:Mice, Inbred BALB C D008969:Molecular Sequence Data D009842:Oligopeptides D017434:Protein Structure, Tertiary D011994:Recombinant Proteins
monoclonal antibodies Human carbonic anhydrase XII inhibition of enzymatic activity