A Comparison of Splenic Pathologic Change and Immune Function in HBV-Related Portal Hypertension and Chinese Budd-Chiari Syndrome Patients with Hypersplenism.
The difference of splenic pathologic alterations and immune function changes in portal hypertension (PHT) with different etiology is unclear. We aimed to investigate the differences between the hypersplenic patients with hepatitis B virus (HBV)-related PHT and Budd-Chiari syndrome (B-CS). A total of 93 patients with hypersplenism due to Chinese primary B-CS (B-CS group), 105 patients with hypersplenism due to HBV-related cirrhosis (HBV/PHT group), and 31 healthy people (control group) were included in this study retrospectively. The peripheral bloods and paraffin sections of the spleen from part of patients were analyzed by flow cytometry and immunohistochemistry. Hypersplenism and PHT were more serious in HBV/PHT group than in B-CS group. In the peripheral blood, the percentages of regulatory T cell (15.1% vs. 8.1% vs. 2.2%, p = 0.0021) and myeloid-derived suppressive cells (2.8% vs. 0.8% vs. 0.9%, p = 0.009) were higher, but CD4+ T and CD8+ T cells were lower in HBV/PHT group compared with B-CS and control groups. In spleen, the percentages of CD4+ T and CD8+ T cells were lower, but CD68+ macrophages were higher in HBV/PHT group than in B-CS group. Moreover, CD86, inducible nitric oxide synthase, Toll-like receptor 4, and tumor necrosis factor-α expression in the spleen, as well as the plasma lipopolysaccharide (LPS) level (677.7 vs. 311.1 vs. 222.1 ng/mL, p = 0.0022), were significantly higher in HBV/PHT group than in B-CS and control groups. The HBV/PHT group showed more severe immunosuppression and immune dysfunction and more substantial hypersplenism and splenic phagocytosis than B-CS group.
['Li|Suxin|S|', 'Huang|Na|N|', 'Dang|Xiaowei|X|', 'Li|Liang|L|', 'Li|Zhenzhen|Z|', 'Zhang|Chen|C|', 'Jiang|An|A|', 'Kong|Guangyao|G|', 'Ji|Fanpu|F|', 'Yang|Jun|J|', 'Li|Zongfang|Z|']
D000328:Adult D006502:Budd-Chiari Syndrome D015496:CD4-Positive T-Lymphocytes D018414:CD8-Positive T-Lymphocytes D002681:China D005260:Female D006509:Hepatitis B D006801:Humans D006971:Hypersplenism D006975:Hypertension, Portal D008103:Liver Cirrhosis D008297:Male D008875:Middle Aged D012189:Retrospective Studies D013154:Spleen D050378:T-Lymphocytes, Regulatory
immune portal hypertension Budd-Chiari syndrome hypersplenism hepatitis B virus