The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus.
The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 μM) with 0.4 μM being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 μM 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures.
['Gačić|Zoran|Z|', 'Kolarević|Stoimir|S|', 'Sunjog|Karolina|K|', 'Kračun-Kolarević|Margareta|M|', 'Paunović|Momir|M|', 'Knežević-Vukčević|Jelena|J|', 'Vuković-Gačić|Branka|B|']
D000818:Animals D000964:Antimetabolites, Antineoplastic D049872:Bivalvia D020552:Comet Assay D004249:DNA Damage D004305:Dose-Response Relationship, Drug D005472:Fluorouracil D005618:Fresh Water D006434:Hemocytes D049881:Unio
Unio pictorum Unio tumidus 5-Fluorouracil Comet assay