Protective effect of Vaccinium myrtillus extract against UVA- and UVB-induced damage in a human keratinocyte cell line (HaCaT cells).
Recently, the field of skin protection have shown a considerable interest in the use of botanicals. Vaccinium myrtillus contains several polyphenols and anthocyanins with multiple pharmacological properties. The purpose of our study was to examine whether a water-soluble V. myrtillus extract (dry matter 12.4%; total polyphenols 339.3mg/100 g fw; total anthocyanins 297.4 mg/100 g fw) was able to reduce UVA- and UVB-induced damage using a human keratinocyte cell line (HaCaT). HaCaT cells were pretreated for 1h with extract in a serum-free medium and then irradiated with UVA (8-40 J/cm(2)) and UVB (0.008-0.72 J/cm(2)) rays. All experiments were performed 24h after the end of irradiation, except for oxidative stress tests. The extract was able to reduce the UVB-induced cytotoxicity and genotoxicity (studied by comet and micronucleous assays) at lower doses. V. myrtillus extract reduced lipid peroxidation UVB-induced, but had no effect against the ROS UVB-produced. With UVA-induced damage V. myrtillus reduced genotoxicity as well as the unbalance of redox intracellular status. Moreover our extract reduced the UVA-induced apoptosis, but had no effect against the UVB one. V. myrtillus extract showed its free radical scavenging properties reducing oxidative stress and apoptotic markers, especially in UVA-irradiated cells.
['Calò|Rossella|R|', 'Marabini|Laura|L|']
D002460:Cell Line D002470:Cell Survival D020552:Comet Assay D004249:DNA Damage D006801:Humans D015603:Keratinocytes D015227:Lipid Peroxidation D053078:Membrane Potential, Mitochondrial D015162:Micronucleus Tests D018384:Oxidative Stress D010936:Plant Extracts D020011:Protective Agents D017382:Reactive Oxygen Species D014466:Ultraviolet Rays D029796:Vaccinium
Vaccinium myrtillus Keratinocytes Oxidative stress Genotoxicity Apoptosis Ultraviolet rays