Protective role of quercetin against manganese-induced injury in the liver, kidney, and lung; and hematological parameters in acute and subchronic rat models.
Manganese (Mn) is an important mineral element required in trace amounts for development of the human body, while over- or chronic-exposure can cause serious organ toxicity. The current study was designed to evaluate the protective role of quercetin (Qct) against Mn-induced toxicity in the liver, kidney, lung, and hematological parameters in acute and subchronic rat models. Male Sprague Dawley rats were divided into control, Mn (100 mg/kg for acute model and 15 mg/kg for subchronic model), and Mn + Qct (25 and 50 mg/kg) groups in both acute and subchronic models. Our result revealed that Mn + Qct groups effectively reduced Mn-induced ALT, AST, and creatinine levels. However, Mn + Qct groups had effectively reversed Mn-induced alteration of complete blood count, including red blood cells, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, platelets, and white blood cells. Meanwhile, the Mn + Qct groups had significantly decreased neutrophil and eosinophil and increased lymphocyte levels relative to the Mn group. Additionally, Mn + Qct groups showed a beneficial effect against Mn-induced macrophages and neutrophils. Our result demonstrated that Mn + Qct groups exhibited protective effects on Mn-induced alteration of GRP78, CHOP, and caspase-3 activities. Furthermore, histopathological observation showed that Mn + Qct groups effectively counteracted Mn-induced morphological change in the liver, kidney, and lung. Moreover, immunohistochemically Mn + Qct groups had significantly attenuated Mn-induced 8-oxo-2'-deoxyguanosine immunoreactivity. Our study suggests that Qct could be a substantially promising organ-protective agent against toxic Mn effects and perhaps against other toxic metal chemicals or drugs.
['Bahar|Entaz|E|', 'Lee|Geum-Hwa|GH|', 'Bhattarai|Kashi Raj|KR|', 'Lee|Hwa-Young|HY|', 'Kim|Hyun-Kyoung|HK|', 'Handigund|Mallikarjun|M|', 'Choi|Min-Kyung|MK|', 'Han|Sun-Young|SY|', 'Chae|Han-Jung|HJ|', 'Yoon|Hyonok|H|']
D000818:Animals D000975:Antioxidants D004195:Disease Models, Animal D000091342:Endoplasmic Reticulum Chaperone BiP D007668:Kidney D008099:Liver D008168:Lung D008264:Macrophages D008297:Male D008345:Manganese D009504:Neutrophils D020011:Protective Agents D011794:Quercetin D051381:Rats D017207:Rats, Sprague-Dawley
quercetin liver kidney lung hematological parameters manganese