A Trifunctional, Modular Biomaterial Coating: Nonadhesive to Bacteria, Chlorhexidine-Releasing and Tissue-Integrating.
Various potential anti-infection strategies can be thought of for biomaterial implants and devices. Permanent, tissue-integrated implants such as artificial joint prostheses require a different anti-infection strategy than, for instance, removable urinary catheters. The different requirements set to biomaterials implants and devices in different clinical applications call for tailor-made strategies. Here, a modular coating-concept for biomaterials is reported, which in its full, trifunctional form comprises nonadhesiveness to bacteria and antimicrobial release, combined with enhanced tissue integration characteristics. Nonadhesiveness to proteins and bacteria is accomplished by a hydrophilic brush coating (Vitrostealth). The antimicrobial release module is constituted by a chlorhexidine releasing poly(ethylene glycol) diacrylamide based-coating that continues to release its antimicrobial content also when underneath the nonadhesive top-coating. The third module, enhancing tissue integration, is realized by the incorporation of the penta-peptide Glycine-Arginine-Glycine-Aspartic acid-Serine (GRGDS) within the nonadhesive top-coating. Modules function in concert or independently of each other. Specifically, tissue integration by the GRGDS-module does not affect the nonadhesiveness of the Vitrostealth-module toward bovine serum albumin and Staphylococcus aureus, while the antimicrobial release module does not affect tissue-integration by the GRGDS-module. Uniquely, using this modular system, tailor-made anti-infection strategies can thus readily be made for biomaterials in different clinical applications.
['Sjollema|Jelmer|J|', 'Keul|Heidrun|H|', 'van der Mei|Henny|H|', 'Dijkstra|René|R|', 'Rustema-Abbing|Minie|M|', 'de Vries|Joop|J|', 'Loontjens|Ton|T|', 'Dirks|Ton|T|', 'Busscher|Henk|H|']
D020106:Acrylamide D000327:Adsorption D000818:Animals D001422:Bacterial Adhesion D002417:Cattle D002448:Cell Adhesion D045744:Cell Line, Tumor D002710:Chlorhexidine D020099:Coated Materials, Biocompatible D065546:Drug Liberation D006801:Humans D009842:Oligopeptides D011092:Polyethylene Glycols D012710:Serum Albumin, Bovine D013211:Staphylococcus aureus
biomaterial coating antimicrobial release tissue integration infection polymer brush