Oxyresveratrol suppresses lipopolysaccharide-induced inflammatory responses in murine macrophages.
Excessive inflammation is considered a critical factor in many human diseases. Oxyresveratrol(trans-2,3',4,5'-tetrahydroxystilbene), a natural hydroxystilbene, has been shown to possess antioxidant and free radical-scavenging activity. In this study, we investigated the effects of oxyresveratrol (OxyR) on the lipopolysaccharide (LPS)-induced production of inflammatory cytokines and mediators and further explored the mechanism of action in RAW264.7 murine macrophage cell line. Production of nitric oxide (NO), prostaglandin E2 (PGE2), messenger RNA (mRNA) and protein expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin 6 (IL-6), and granulocyte macrophage colony-stimulating factor (GM-CSF), phosphorylation of mitogen-activated protein kinases (MAPKs; extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38), and the activation of nuclear factor κ-light chain enhancer of activated B cells (NFκB) with OxyR were assayed in LPS-stimulated RAW264.7 cells. OxyR inhibited the productions of NO, PGE2, IL-6, and GM-CSF significantly in LPS-stimulated RAW264.7 cells. OxyR suppressed mRNA and protein expressions of iNOS, COX-2, IL-6, and GM-CSF in LPS-stimulated RAW264.7 cells. OxyR suppressed the phosphorylation of Akt and JNK and p38 MAPKs and the translocation of NFκB p65 subunit into the nucleus. These results indicate that OxyR inhibits LPS-stimulated inflammatory responses though the blocking of MAPK and NFκB signaling pathway in macrophages, and suggest that OxyR possesses anti-inflammatory effects.
['Lee|H S|HS|', 'Kim|D H|DH|', 'Hong|J E|JE|', 'Lee|J-Y|JY|', 'Kim|E J|EJ|']
D000818:Animals D002460:Cell Line D051546:Cyclooxygenase 2 D015232:Dinoprostone D016178:Granulocyte-Macrophage Colony-Stimulating Factor D007249:Inflammation D015850:Interleukin-6 D008070:Lipopolysaccharides D008264:Macrophages D051379:Mice D009569:Nitric Oxide D052247:Nitric Oxide Synthase Type II D010766:Phosphorylation D010936:Plant Extracts D011494:Protein Kinases D013267:Stilbenes
Oxyresveratrol mitogen-activated protein kinase NFκB Akt anti-inflammation granulocyte macrophage-colony stimulating factor