TIM‑3 inhibits PDGF‑BB‑induced atherogenic responses in human artery vascular smooth muscle cells.
Increasing evidence suggests that T‑cell immunoglobulin and mucin domain 3 (TIM‑3) displays anti‑atherosclerotic effects, but its role in vascular smooth muscle cells (VSMCs) has not been reported. The present study aimed to investigate the function of TIM‑3 and its roles in human artery VSMCs (HASMCs). A protein array was used to investigate the TIM‑3 protein expression profile, which indicated that TIM‑3 expression was increased in the serum of patients with lower extremity arteriosclerosis obliterans disease (LEAOD) compared with healthy individuals. Immunohistochemistry and western blotting of arterial tissue further revealed that TIM‑3 expression was increased in LEAOD artery tissue compared with normal artery tissue. Additionally, platelet‑derived growth factor‑BB (PDGF‑BB) displayed a positive correlation with TIM‑3 expression in HASMCs. TIM‑3 decreased the migration and proliferation of PDGF‑BB‑induced HASMCs, and anti‑TIM‑3 blocked the effects of TIM‑3. The effect of TIM‑3 on the proliferation and migration of HASMCs was further investigated using LV‑TIM‑3‑transduced cells. The results revealed that TIM‑3 also inhibited PDGF‑BB‑induced expression of the inflammatory factors interleukin‑6 and tumor necrosis factor‑α by suppressing NF‑κB activation. In summary, the present study revealed that TIM‑3 displayed a regulatory role during the PDGF‑BB‑induced inflammatory reaction in HASMCs, which indicated that TIM‑3 may display anti‑atherosclerotic effects.
['Lian|Chong|C|', 'Wang|Zhecun|Z|', 'Qiu|Jiacong|J|', 'Jiang|Baohong|B|', 'Lv|Junbing|J|', 'He|Rongzhou|R|', 'Liu|Ruiming|R|', 'Li|Wen|W|', 'Wang|Jinsong|J|', 'Wang|Shenming|S|']
D000368:Aged D001158:Arteries D001162:Arteriosclerosis Obliterans D050197:Atherosclerosis D000077214:Becaplermin D002460:Cell Line D002465:Cell Movement D049109:Cell Proliferation D005260:Female D000072597:Hepatitis A Virus Cellular Receptor 2 D006801:Humans D015850:Interleukin-6 D035002:Lower Extremity D008297:Male D008875:Middle Aged D009131:Muscle, Smooth, Vascular D016328:NF-kappa B D040081:Protein Array Analysis D059467:Transcriptome D014409:Tumor Necrosis Factor-alpha
human artery vascular smooth muscle cells migration proliferation T-cell immunoglobulin and mucin domain 3 atherosclerosis inflammation platelet-derived growth  factor-BB