Contribution of structural accessibility to the cooperative relationship of TF-lncRNA in myopia.
Transcriptional regulation is associated with complicated mechanisms including multiple molecular interactions and collaborative drive. Long noncoding RNAs (lncRNAs) have highly structured characteristics and play vital roles in the regulation of transcription in organisms. However, the specific contributions of conformation feature and underlying molecular mechanisms are still unclear. In the present paper, a hypothesis regarding molecular structure effect is presented, which proposes that lncRNAs fold into a complex spatial architecture and act as a skeleton to recruit transcription factors (TF) targeted binding, and which is involved in cooperative regulation. A candidate set of TF-lncRNA coregulation was constructed, and it was found that structural accessibility affected molecular binding force. In addition, transcription factor binding site (TFBS) regions of myopia-related lncRNA transcripts were disturbed, and it was discovered that base mutations affected the occurrence of significant molecular allosteric changes in important elements and variable splicing regions, mediating the onset and development of myopia. The results originated from structureomics and interactionomics and created conditions for systematic research on the mechanisms of structure-mediated TF-lncRNA coregulation in transcriptional regulation. Finally, these findings will help further the understanding of key regulatory roles of molecular allostery in cell physiological and pathological processes.
['Wang|Hong|H|', 'Li|Jing|J|', 'Wang|Siyu|S|', 'Lu|Xiaoyan|X|', 'Zhang|Guosi|G|', 'Zhuang|Youyuan|Y|', 'Li|Liansheng|L|', 'Wang|Wencan|W|', 'Lin|Peng|P|', 'Chen|Chong|C|', 'Wang|Hao|H|', 'Chen|Qi|Q|', 'Jiang|Yongshuai|Y|', 'Qu|Jia|J|', 'Xu|Liangde|L|']
D001665:Binding Sites D020869:Gene Expression Profiling D005786:Gene Expression Regulation D053263:Gene Regulatory Networks D006801:Humans D008958:Models, Molecular D009216:Myopia D009690:Nucleic Acid Conformation D020641:Polymorphism, Single Nucleotide D011485:Protein Binding D000072417:Protein Domains D059370:RNA Folding D062085:RNA, Long Noncoding D014157:Transcription Factors
structural accessibility lncRNAs cooperative regulation allosteric effects secondary structure