Fabricating three-dimensional carbohydrate hydrogel microarray for lectin-mediated bacterium capturing.
Herein, a three-dimensional carbohydrate modified polyacrylamide hydrogel microarray (3D carbohydrate hydrogel microarray) has been fabricated and employed as micro-reactor for capturing Escherichia coli (E. coli) by multivalent binding of concanavalin A (Con A) with O-antigen on the cellular surface of E. coli and immobilized monosaccharides on hydrogel spot, and the interactions of type 1 fimbriae of E. coli with immobilized monosaccharides. Because of the transparent performance of polyacrylamide hydrogel, the captured E. coli can be directly observed by a conventional microscope under a bright-field mode. The experimental result demonstrates that α-D-mannopyranoside (Man-α) modified hydrogel surface shows high efficiency of E. coli capturing. The 3D Man-α hydrogel microarray-based assay shows reasonable low detection limit (1.0×10(4) cells/mL) and large dynamic range (1.0×10(5) to 1.0×10(9)cells/mL) for detecting E. coli. In addition, bacterial adhesion inhibition assay has been demonstrated by the interactions of E. coli with ten saccharides, and satisfactory results have been obtained.
['Liu|Xia|X|', 'Lei|Zhen|Z|', 'Liu|Fuyao|F|', 'Liu|Dianjun|D|', 'Wang|Zhenxin|Z|']
D001422:Bacterial Adhesion D019149:Bioreactors D002469:Cell Separation D004867:Equipment Design D019544:Equipment Failure Analysis D004926:Escherichia coli D020100:Hydrogels D037102:Lectins D046228:Microarray Analysis D046210:Microfluidic Analytical Techniques D009005:Monosaccharides D066330:Printing, Three-Dimensional
Bacterium capturing Polyacrylamide hydrogel Saccharides Lectin Three-dimensional microarray