Transcriptome response to infraorbital nerve transection in the gonadally intact male rat barrel cortex: RNA-seq.
The effects of infraorbital nerve (ION) transection on gene expression in the adult male rat barrel cortex were investigated using RNA sequencing. After a 24-hour survival duration, 98 genes were differentially regulated by ION transection. Differentially expressed genes suggest changes in neuronal activity, excitability, and morphology. The production of mRNA for neurotrophins, including brain-derived neurotrophin factor (BNDF), was decreased following ION transection. Several potassium channels showed decreased mRNA production, whereas a sodium channel (Na(V)β4) associated with burst firing showed increased mRNA production. The results may have important implications for phantom-limb pain and complex regional pain syndrome. Future experiments should determine the extent to which changes in RNA result in changes in protein expression, in addition to utilizing laser capture microdissection techniques to differentiate between neuronal and glial cells.
['Orczyk|John J|JJ|', 'Sethia|Rishabh|R|', 'Doster|Dominique|D|', 'Garraghty|Preston E|PE|']
D000818:Animals D015870:Gene Expression D008297:Male D008442:Maxillary Nerve D009473:Neuronal Plasticity D012333:RNA, Messenger D017207:Rats, Sprague-Dawley D012683:Sensory Deprivation D013003:Somatosensory Cortex D059467:Transcriptome D014738:Vibrissae
CLC Main Workbench (RRID:OMICS_01813) Ingenuity Pathway Analysis (RRID:nif-0000-33144) Sprague-Dawley rats (RRID:RGD_70508) cerebral cortex injury plasticity