Growth associated protein (GAP-43): cloning and the development of a sensitive ELISA for neurological disorders.
GAP-43 has been studied in the rodent and mammalian brain and shown to be present specifically in areas undergoing axonal elongation and synapse formation. GAP-43 was cloned using the baculovirus expression system and purified. A sandwich ELISA was developed using the recombinant GAP-43 as standard and validated. CSF GAP-43 levels were analysed in benign intracranial hypertension, movement disorders, multiple sclerosis, neuropathy, CNS infections, motor neuron disease, and headache (neurological controls). GAP-43 levels were low in all disorders analysed (in particular motor neuron disease; p=0.001, and movement disorders and multiple sclerosis; p<0.0001) compared to controls, aside from CNS infections. GAP-43 is preferentially reduced in the CSF of neurological disorders associated with neurodegeneration.
['Gnanapavan|Sharmilee|S|', 'Yousaf|Nasim|N|', 'Heywood|Wendy|W|', 'Grant|Donna|D|', 'Mills|Kevin|K|', 'Chernajovsky|Yuti|Y|', 'Keir|Geoff|G|', 'Giovannoni|Gavin|G|']
D000818:Animals D019976:Cloning, Organism D004797:Enzyme-Linked Immunosorbent Assay D019922:GAP-43 Protein D006801:Humans D051379:Mice D009422:Nervous System Diseases
GAP-43 ELISA CSF Biomarker Neuroplasticity