Resolvin D1 and E1 promote resolution of inflammation in rat cardiac fibroblast in vitro.
Cardiac fibroblasts (CFs) have a key role in the inflammatory response after cardiac injury and are necessary for wound healing. Resolvins are potent agonists that control the duration and magnitude of inflammation. They decrease mediators of pro-inflammatory expression, reduce neutrophil migration to inflammation sites, promote the removal of microbes and apoptotic cells, and reduce exudate. However, whether resolvins can prevent pro-inflammatory-dependent effects in CFs is unknown. Thus, the present work was addressed to study whether resolvin D1 and E1 (RvD1 and RvE1) can prevent pro-inflammatory effects on CFs after lipopolysaccharide (LPS) challenge. For this, CFs were stimulated with LPS, in the presence or absence of RvD1 or RvE1, to analyze its effects on intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion protein 1 (VCAM-1), monocyte adhesion and the cytokine levels of tumor necrosis factor alpha (TNF-α), interleukin-6(IL-6), interleukin-1beta (IL-1β), monocyte chemoattractant protein-1 (MCP-1) and interleukin-10 (IL-10). Our results showed that CFs are expressing ALX/FPR2 and ChemR23, RvD1 and RvE1 receptors, respectively. RvD1 and RvE1 prevent the increase of ICAM-1 and VCAM-1 protein levels and the adhesion of spleen mononuclear cells to CFs induced by LPS. Finally, RvD1, but not RvE1, prevents the LPS-induced increase of IL-6, MCP-1, TNF-α, and IL-10. In conclusion, our findings provide evidence that in CFs, RvD1 and RvE1 might actively participate in the prevention of inflammatory response triggered by LPS.
['Salas-Hernández|Aimeé|A|', 'Espinoza-Pérez|Claudio|C|', 'Vivar|Raúl|R|', 'Espitia-Corredor|Jenaro|J|', 'Lillo|José|J|', 'Parra-Flores|Pablo|P|', 'Sánchez-Ferrer|Carlos F|CF|', 'Peiró|Concepción|C|', 'Díaz-Araya|Guillermo|G|http://orcid.org/0000-0001-7960-8104']
D000818:Animals D002465:Cell Movement D016207:Cytokines D004281:Docosahexaenoic Acids D015118:Eicosapentaenoic Acid D005347:Fibroblasts D005786:Gene Expression Regulation D006335:Heart Injuries D006801:Humans D007249:Inflammation D053583:Interleukin-1beta D008070:Lipopolysaccharides D009504:Neutrophils D051381:Rats D014409:Tumor Necrosis Factor-alpha D019010:Vascular Cell Adhesion Molecule-1 D014945:Wound Healing
Resolvin D1 Cardiac fibroblasts Cytokines Resolvin E1