Rutin attenuates neuroinflammation in spinal cord injury rats.
BACKGROUND
Neuroinflammatory responses involve the activation of the interleukin (IL) -1β and IL-18. Processing and activation of the pro-inflammatory IL require NLRP3 inflammasome activation. Rutin can protect spinal cord against damage, but the potential mechanisms underlying remain unknown. Here, we investigated the molecular mechanisms of rutin-mediated neuroprotection in a rat model of spinal cord injury (SCI).


MATERIALS AND METHODS
One hundred twenty female Sprague-Dawley rats were randomly assigned to four groups: sham group, SCI group, SCI + Rutin50 group, and the SCI + Rutin100 group. The influences of rutin on inflammatory marker levels, histologic alterations, and locomotion scale were analyzed.


RESULTS
SCI significantly increased the expression of the NLRP3, ASC, IL-1β, IL-18, and tumor necrosis factor-alpha. Rutin significantly reduced the levels of reactive oxygen species, malondialdehyde, NLRP3, ASC, caspase-1, IL-1β, IL-18, and tumor necrosis factor-alpha. Furthermore, rutin administration significantly attenuated histologic alteration and improved locomotion recovery.


CONCLUSIONS
Our data provide clear evidence that rutin attenuates tissue damage and improves locomotion recovery, and the mechanism may be related to the alleviation of inflammation and oxidative stress.
['Wu|Jiang|J|', 'Maoqiang|Li|L|', 'Fan|He|H|', 'Zhenyu|Bian|B|', 'Qifang|He|H|', 'Xuepeng|Wang|W|', 'Liulong|Zhu|Z|']
D000818:Animals D015415:Biomarkers D015153:Blotting, Western D005260:Female D058847:Inflammasomes D008124:Locomotion D018696:Neuroprotective Agents D011897:Random Allocation D051381:Rats D017207:Rats, Sprague-Dawley D012431:Rutin D013116:Spinal Cord D013119:Spinal Cord Injuries D016896:Treatment Outcome
Rutin Spinal cord injury NLRP3 inflammasome Reactive oxygen species Inflammation