Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins.
The characterisation of fish blood proteomes is important for comparative studies of seminal and blood proteins as well as for the analysis of fish immune mechanisms and pathways. In this study, LC-MS/MS and 2D-DIGE were applied to compare rainbow trout seminal (SP) and blood plasma (BP) proteomes. The 54 differentially abundant proteins identified in SP are involved in a variety of signalling pathways, including protein ubiquitination, liver X receptor/retinoid X receptor (LXR/RXR) and farnesoid X receptor activation, cell cycle and acute phase signalling. These findings may indicate the prevalence of acute phase signalling pathways in trout SP, and its essential role in protecting spermatozoa and reproductive tissues. Our study provides the first in-depth analysis of the trout BP proteome, with a total of 119 proteins identified. The major proteins of rainbow trout BP were recognised as acute phase proteins. Analysis of BP proteins indicated that acute phase response signalling, the complement system, liver X receptor/retinoid X receptor and farnesoid X receptor activation and the coagulation system are the top canonical pathways. This study enhances knowledge of the blood origin of trout SP proteins and understanding of fish reproductive biology. Our results provide new insight into blood proteins specifically important for fish physiology and innate immunity. The mass spectrometry data are available via ProteomeXchange with the identifier PXD005988 and https://doi.org/10.6019/PXD005988.
['Nynca|Joanna|J|', 'Arnold|Georg|G|', 'Fröhlich|Thomas|T|', 'Ciereszko|Andrzej|A|']
D000818:Animals D001798:Blood Proteins D029941:Fish Proteins D008297:Male D017686:Oncorhynchus mykiss D040901:Proteomics D029589:Seminal Plasma Proteins D015398:Signal Transduction D053719:Tandem Mass Spectrometry D057825:Two-Dimensional Difference Gel Electrophoresis
Blood plasma Seminal plasma Fish 2D-DIGE Mass spectrometry