CRIP1 promotes cell migration, invasion and epithelial-mesenchymal transition of cervical cancer by activating the Wnt/β‑catenin signaling pathway.
Cervical cancer (CC) is the third most common cancer and the fourth leading cause of malignancy-related mortality in women worldwide. In addition, epithelial-mesenchymal transition (EMT) has been generally studied in tumor metastasis researches in recent years. Cysteine-rich intestinal protein 1 (CRIP1) is differently expressed in human cancer cells. However, the role it plays in CC has not been revealed at present. Preliminary experiments have shown that CRIP1 had a higher expression in CC tissues, compared with adjacent noncancerous tissues. Real-time PCR and western blot were performed to analyze CRIP1 expression in CC cell lines. CRIP1 transient transfection vector and siRNA were constructed. Further analysis revealed the promotion effects of CRIP1 on the cell migration and invasion of CC in vitro (P < 0.01). In addition, western blot was performed to show that CRIP1 mediates EMT by means of EMT marker detection. The expression of CRIP1 and β‑catenin in CC tissues was analyzed by immunohistochemistry (IHC). Interestingly, CRIP1 and β‑catenin were both highly expressed in CC tissues (P < 0.01). Furthermore, CRIP1 increased the protein expression level of c-myc, cyclinD-1 and cytoplasmic β‑catenin, which are indicators for activating the Wnt/β‑catenin signaling pathway. In conclusion, CRIP1 promotes cell migration and invasion, mediates EMT and activates the Wnt/β‑catenin signaling pathway in CC.
['Zhang|Lan-Zhi|LZ|', 'Huang|Li-Yan|LY|', 'Huang|An-Liang|AL|', 'Liu|Jin-Xing|JX|', 'Yang|Fan|F|']
D000328:Adult D002352:Carrier Proteins D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D019938:Cyclin D1 D003593:Cytoplasm D058750:Epithelial-Mesenchymal Transition D005260:Female D020869:Gene Expression Profiling D015972:Gene Expression Regulation, Neoplastic D006367:HeLa Cells D006801:Humans D060588:LIM Domain Proteins D008875:Middle Aged D009361:Neoplasm Invasiveness D016271:Proto-Oncogene Proteins c-myc D015398:Signal Transduction D002583:Uterine Cervical Neoplasms D051153:Wnt Proteins D060449:Wnt Signaling Pathway D051176:beta Catenin
CRIP1 Invasion Epithelial-mesenchymal transition Cervical cancer Wnt/β‑catenin