Reciprocal and dynamic polarization of planar cell polarity core components and myosin.
The Ciona notochord displays planar cell polarity (PCP), with anterior localization of Prickle (Pk) and Strabismus (Stbm). We report that a myosin is polarized anteriorly in these cells and strongly colocalizes with Stbm. Disruption of the actin/myosin machinery with cytochalasin or blebbistatin disrupts polarization of Pk and Stbm, but not of myosin complexes, suggesting a PCP-independent aspect of myosin localization. Wash out of cytochalasin restored Pk polarization, but not if done in the presence of blebbistatin, suggesting an active role for myosin in core PCP protein localization. On the other hand, in the pk mutant line, aimless, myosin polarization is disrupted in approximately one third of the cells, indicating a reciprocal action of core PCP signaling on myosin localization. Our results indicate a complex relationship between the actomyosin cytoskeleton and core PCP components in which myosin is not simply a downstream target of PCP signaling, but also required for PCP protein localization.
['Newman-Smith|Erin|E|', 'Kourakis|Matthew J|MJ|', 'Reeves|Wendy|W|', 'Veeman|Michael|M|', 'Smith|William C|WC|']
D008841:Actin Cytoskeleton D000818:Animals D001426:Bacterial Proteins D002467:Cell Nucleus D016764:Cell Polarity D002938:Ciona intestinalis D003571:Cytochalasin B D004625:Embryo, Nonmammalian D005326:Fetal Proteins D015870:Gene Expression D018507:Gene Expression Regulation, Developmental D006576:Heterocyclic Compounds, 4 or More Rings D008164:Luminescent Proteins D009218:Myosins D009672:Notochord D020033:Protein Isoforms D011993:Recombinant Fusion Proteins D015398:Signal Transduction D020825:T-Box Domain Proteins D020663:ras Guanine Nucleotide Exchange Factors
planar cell polarity Ciona developmental biology notohcord stem cells