KB-R7943 Inhibits the Mitochondrial Ca2+ Uniporter but Not Na+-Ca2+ Exchanger in Cardiomyocyte-Derived H9c2 Cells.
The effect of KB-R7943, an inhibitor of the plasmalemmal Na+-Ca2+ exchanger, on mitochondrial Ca2+ transporters was examined with membrane-permeabilized cardiomyocyte-derived H9c2 cells expressing the fluorescent Ca2+ indicator, yellow cameleon 3.1, in the mitochondria. KB-R7943, as well as ruthenium red, inhibited the rise in mitochondrial Ca2+ on increasing the extramitochondrial Ca2+ concentration from 0 nM to 300 nM. CGP-37157, but not KB-R7943, inhibited the decline in mitochondrial Ca2+on return to Ca2+ free extramitochondrial solution. These results indicated that KB-R7943 has inhibitory effects on the mitochondrial Ca2+ uniporter, but not on the mitochondrial Na+-Ca2+ exchanger.
['Namekata|Iyuki|I|', 'Odaka|Ryosuke|R|', 'Hamaguchi|Shogo|S|', 'Tanaka|Hikaru|H|']
D000818:Animals D002118:Calcium D015220:Calcium Channels D002462:Cell Membrane D002478:Cells, Cultured D002998:Clonazepam D008928:Mitochondria D032383:Myocytes, Cardiac D051381:Rats D012964:Sodium D019831:Sodium-Calcium Exchanger D013841:Thiazepines D013890:Thiourea
KB-R7943 Ca2+ uniporter mitochondria Ca2+ imaging Na+–Ca2+ exchanger cardiomyocyte