Andrographolide ameliorates OVA-induced lung injury in mice by suppressing ROS-mediated NF-κB signaling and NLRP3 inflammasome activation.
In this study, we attempted to explore the effect and possible mechanism of Andrographolide on OVA-induced asthma. OVA challenge induced significant airway inflammatory cell recruitment and lung histological alterations, which were ameliorated by Andrographolide. The protein levels of cytokines in bron-choalveolar fluid (BALF) and serum were reduced by Andrographolide administration as well as the mRNA levels in lung tissue. Mechanically, Andrographolide markedly hampered the activation of nuclear factor-κB (NF-κB) and NLRP3 inflammasome both in vivo and vitro thus decreased levels of TNF-α and IL-1β. Finally, we confirmed that ROS scavenging was responsible for Andrographolide's inactivation of NF-κB and NLRP3 inflammasome signaling. Our study here revealed the effect and possible mechanism of Andrographolide on asthma, which may represent a new therapeutic approach for treating this disease.
['Peng|Shuang|S|', 'Gao|Jian|J|', 'Liu|Wen|W|', 'Jiang|Chunhong|C|', 'Yang|Xiaoling|X|', 'Sun|Yang|Y|', 'Guo|Wenjie|W|', 'Xu|Qiang|Q|']
D000818:Animals D018927:Anti-Asthmatic Agents D000893:Anti-Inflammatory Agents D000975:Antioxidants D001249:Asthma D001992:Bronchoalveolar Lavage Fluid D016207:Cytokines D004195:Disease Models, Animal D004224:Diterpenes D004305:Dose-Response Relationship, Drug D005260:Female D005786:Gene Expression Regulation D058847:Inflammasomes D008168:Lung D008810:Mice, Inbred C57BL D016328:NF-kappa B D000071199:NLR Family, Pyrin Domain-Containing 3 Protein D010047:Ovalbumin D018384:Oxidative Stress D011014:Pneumonia D017382:Reactive Oxygen Species D015398:Signal Transduction
andrographolide NF-κB NLRP3 asthma ROS Immune response Immunity Immunology and Microbiology Section