Pentraxin 3 promotes airway inflammation in experimental asthma.
BACKGROUND
Pentraxin 3 (PTX3) regulates multiple aspects of innate immunity and tissue inflammation. Recently, it has been reported that PTX3 deficiency enhances interleukin (IL)-17A-dominant pulmonary inflammation in an ovalbumin (OVA)-induced mouse asthma model. However, whether PTX3 treatment would provide protection against allergic airway inflammation has not been clearly elucidated. The goal of this study was to further investigate the effect of recombinant PTX3 administration on the phenotype of asthma.


METHODS
C57BL/6 J mice were sensitized and challenged with OVA to induce eosinophilic asthma model, as well as sensitized with OVA plus LPS and challenged with OVA to induce neutrophilic asthma model. We evaluated effect of recombinant PTX3 on asthma phenotype through both asthma models. The bronchoalveolar lavage fluid (BALF) inflammatory cells and cytokines, airway hyperresponsiveness, and pathological alterations of the lung tissues were assessed.


RESULTS
In both eosinophilic and neutrophilic asthma models, PTX3 treatment provoked airway hyperresponsiveness, concomitant with increased inflammatory cytokines IL-4, IL-17, eotaxin, and transforming growth factor (TGF)-β1 and aggravated airway accumulation of inflammatory cells, especially eosinophils and neutrophils. In histological analysis of the lung tissue, administration of PTX3 promoted inflammatory cells infiltration, mucus production, and collagen deposition. In addition, PTX3 also significantly enhanced STAT3 phosphorylation in lung tissue.


CONCLUSION
Our results show that exogenous PTX3 can exacerbate multiple asthmatic features by promoting both eosinophils and neutrophils lung infiltration and provide new evidence to better understand the complex role of PTX3 in allergic airway inflammation.
['Gao|Pengfei|P|', 'Tang|Kun|K|', 'Lu|Yanjiao|Y|', 'Huang|Zhenli|Z|', 'Wang|Shanshan|S|', 'Wang|Meijia|M|', 'Wang|Jianmiao|J|', 'Zhao|Jianping|J|', 'Xie|Jungang|J|http://orcid.org/0000-0001-9037-3027']
D000818:Animals D001249:Asthma D002097:C-Reactive Protein D005260:Female D018836:Inflammation Mediators D051379:Mice D008810:Mice, Inbred C57BL D009419:Nerve Tissue Proteins D010047:Ovalbumin
Airway inflammation Asthma PTX3 Eosinophil Neutrophil