MRI-Visible Nanovehicle for Efficient siRNA Delivery.
Visualizing siRNA delivery through medical imaging methods has drawn much attentions in recent gene therapy studies. Among them, iron oxide-based magnetic resonance imaging (MRI) is regarded as one of the most promising imaging modalities for its high spatial resolution as well as deep penetration and real-time properties. In this chapter, a detailed protocol of an amphiphilic superparamagnetic iron oxide (SPIO) nanovehicle-based siRNA delivery is described, mainly focusing on SPIO/siRNA complexes formation and characterization, in vitro and in vivo siRNA delivery, MRI study of the delivery and transfection efficiency evaluation.
['Jin|Rongrong|R|', 'Zhu|Wencheng|W|', 'Lin|Gan|G|', 'Liu|Gang|G|', 'Ai|Hua|H|']
D000818:Animals D001943:Breast Neoplasms D003287:Contrast Media D005260:Female D005290:Ferric Compounds D015972:Gene Expression Regulation, Neoplastic D006801:Humans D061986:MCF-7 Cells D008279:Magnetic Resonance Imaging D051379:Mice D011094:Polyethyleneimine D034622:RNA Interference D034741:RNA, Small Interfering D012107:Research Design D014162:Transfection D057188:Workflow
Nanovehicle siRNA delivery MRI SPIO Cell transfection