Advanced paramagnetic resonance spectroscopies of iron-sulfur proteins: Electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM).
The advanced electron paramagnetic resonance (EPR) techniques, electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) spectroscopies, provide unique insights into the structure, coordination chemistry, and biochemical mechanism of nature's widely distributed iron-sulfur cluster (FeS) proteins. This review describes the ENDOR and ESEEM techniques and then provides a series of case studies on their application to a wide variety of FeS proteins including ferredoxins, nitrogenase, and radical SAM enzymes. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.
['Cutsail|George E|GE|', 'Telser|Joshua|J|', 'Hoffman|Brian M|BM|']
D004578:Electron Spin Resonance Spectroscopy D005288:Ferredoxins D007501:Iron D007506:Iron-Sulfur Proteins D008956:Models, Chemical D008958:Models, Molecular D015394:Molecular Structure D009591:Nitrogenase D017434:Protein Structure, Tertiary D013455:Sulfur
Electron nuclear double resonance (ENDOR) Electron spin echo envelope modulation (ESEEM) Advanced paramagnetic resonance spectroscopy of iron–sulfur proteins