Correlation of gene expression with bladder capacity in interstitial cystitis/bladder pain syndrome.
PURPOSE
Interstitial cystitis and bladder pain syndrome are terms used to describe a heterogeneous chronic pelvic and bladder pain disorder. Despite its significant prevalence, our understanding of disease etiology is poor. We molecularly characterized interstitial cystitis/bladder pain syndrome and determined whether there are clinical factors that correlate with gene expression.


MATERIALS AND METHODS
Bladder biopsies from female subjects with interstitial cystitis/bladder pain syndrome and female controls without signs of the disease were collected and divided into those with normal and low anesthetized bladder capacity, respectively. Samples then underwent RNA extraction and microarray assay. Data generated by these assays were analyzed using Omics Explorer (Qlucore, Lund, Sweden), GeneSifter® Analysis Edition 4.0 and Ingenuity® Pathway Analysis to determine similarity among samples within and between groups, and measure differentially expressed transcripts unique to each phenotype.


RESULTS
A total of 16 subjects were included in study. Principal component analysis and unsupervised hierarchical clustering showed clear separation between gene expression in tissues from subjects with low compared to normal bladder capacity. Gene expression in tissue from patients with interstitial cystitis/bladder pain syndrome who had normal bladder capacity did not significantly differ from that in controls without interstitial cystitis/bladder pain syndrome. Pairwise analysis revealed that pathways related to inflammatory and immune response were most involved.


CONCLUSIONS
Microarray analysis provides insight into the potential pathological condition underlying interstitial cystitis/bladder pain syndrome. This pilot study shows that patients with this disorder who have low compared to normal bladder capacity have significantly different molecular characteristics, which may reflect a difference in disease pathophysiology.
['Colaco|Marc|M|', 'Koslov|David S|DS|', 'Keys|Tristan|T|', 'Evans|Robert J|RJ|', 'Badlani|Gopal H|GH|', 'Andersson|Karl-Erik|KE|', 'Walker|Stephen J|SJ|']
D000293:Adolescent D000328:Adult D000368:Aged D000369:Aged, 80 and over D001706:Biopsy D002908:Chronic Disease D059350:Chronic Pain D018856:Cystitis, Interstitial D003558:Cystoscopy D005260:Female D005500:Follow-Up Studies D005786:Gene Expression Regulation D006801:Humans D046228:Microarray Analysis D008875:Middle Aged D017699:Pelvic Pain D010641:Phenotype D010865:Pilot Projects D015995:Prevalence D011446:Prospective Studies D012313:RNA D013548:Sweden D013577:Syndrome D001743:Urinary Bladder D055815:Young Adult
gene expression interstitial cystitis pain microarray analysis urinary bladder