Datura suaveolens and Verbena tenuisecta mediated silver nanoparticles, their photodynamic cytotoxic and antimicrobial evaluation.
Biogenic production of nanoparticles is eco-friendly, less expensive method with various medical and biological applications. Nanotechnology along with photodynamic therapy is gaining tremendous importance with enhanced efficacy. The present work was aimed to evaluate methanolic extracts and nanoparticles of two selected plants (Datura suavolens and Verbina tenuisecta) for cytotoxic photodynamic, antioxidant and antimicrobial study. Both extract and silver (5 mM) nanoparticles of Datura plant showed significant activities against bacterial strains. Maximum ZOI of 27.3 ± 1.6 mm was observed with nanoparticles of Datura branches with minimum inhibitory (MIC) value of 32 µg/ml. In case of antifungal and antioxidant assay samples were moderately active. Silver nanoparticles and extracts were effective against rhabdomyosarcoma cell line with lowest IC50 value of 42.5 ± 0.6 μg/ml and percent viability of 25.6 ± 1.3 of Verbena tenuisecta. However, nanoparticles of Datura leaves and branches were more potent with IC50 value of 2.4 ± 0.9 μg/ml and 7.8 ± 1.1 μg/ml respectively. The result of photodynamic study showed that efficacy of photosensitizer was enhanced and percent viability reduced when nanoparticles used as an adjunct. The color change and UV spectra (415‒425 nm) indicated the production of nanoparticles. Fourier transform infrared spectroscopy (FTIR) spectra showed presence of different functional groups e.g., hydroxyl, carbonyl and amino. Nanoparticles are sphenoid in morphology and size ranges between 20-150 nm. Current study showed these silver nanoparticles can be used as cytotoxic agent in photodynamic therapy and can play a critical role to establish medicinal potential of selected plants.
['Ashraf|Ahsan|A|', 'Fatima|Nighat|N|', 'Shahzadi|Irum|I|', 'Tariq|Hina|H|', 'Shahzadi|Anam|A|', 'Yameen|Muhammad Arfat|MA|', 'Iqbal|Jamshed|J|', 'Rafi|Muhammad|M|']
D000890:Anti-Infective Agents D000970:Antineoplastic Agents D000975:Antioxidants D045744:Cell Line, Tumor D002470:Cell Survival D032302:Datura D006801:Humans D053768:Metal Nanoparticles D000432:Methanol D008826:Microbial Sensitivity Tests D017319:Photosensitizing Agents D010936:Plant Extracts D018515:Plant Leaves D012834:Silver D032412:Verbena
Nanoparticles Cytotoxicity Antimicrobial Photodynamic therapy