Microcrystalline cellulose based matrix solid phase dispersion microextration for isomeric triterpenoid acids in loquat leaves by ultrahigh-performance liquid chromatography and quadrupole time-of-flight mass spectrometry.
An analytical procedure based on matrix solid phase dispersion (MSPD) microextration and ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was developed for the determination of isomeric triterpenoid acids (maslinic acid, corosolic acid, oleanolic acid and ursolic acid) in loquat leaves. Microcrystalline cellulose was used for the first time as a solid sorbent in MSPD microextration. Compared with the traditional extraction methods, the proposed method possessed the advantages of shorter extraction time, and lower consumption of sample, sorbent and organic solvent. The MSPD parameters that influenced the extraction efficiency of isomeric analytes were investigated and optimized in detail. Under the optimized conditions, good linearity was obtained with correlation coefficients higher than 0.9990. The limits of detection and quantification were 19.6-51.6μg/kg and 65.3-171.8μg/kg, respectively. Meanwhile, the recoveries obtained for all the analytes were ranging from 90.1% to 107.5%. Finally, the optimized method was successfully applied for analyzing these isomeric acids in loquat leaves samples obtained from different cultivated areas.
['Cao|Jun|J|', 'Peng|Li-Qing|LQ|', 'Xu|Jing-Jing|JJ|']
D002482:Cellulose D002851:Chromatography, High Pressure Liquid D031983:Eriobotrya D007536:Isomerism D057230:Limit of Detection D009828:Oleanolic Acid D018515:Plant Leaves D052616:Solid Phase Extraction D012997:Solvents D053719:Tandem Mass Spectrometry D014315:Triterpenes
Microcrystalline cellulose Matrix solid phase dispersion microextration Isomeric triterpenoid acids Loquat leaves Ultrahigh-performance liquid chromatography Quadrupole time-of-flight mass spectrometry