Expression and functional characterization of interferon regulatory factors 4, 8, and 9 in large yellow croaker (Larimichthys crocea).
Interferon regulatory factor (IRF)-4, 8, and 9 are essential in host defense against pathogens. Here, the full-length coding sequence (CDS), protein structure, and immune response of IRF4/8/9 (lc IRF4/8/9) were characterized in large yellow croaker (Larimichthys crocea). The open reading frame of lcIRF4, lcIRF8 and lcIRF9 encoded putative proteins of 463,422 and 406 amino acids, respectively. These IRFs share high sequence homology with other vertebrate IRFs and were constitutively expressed in all examined tissues. IRFs were upregulated following stimulation with Vibrio anguillarum in the liver, spleen, and kidney. These results suggest that IRF4/8/9 are vital in the defense of L. crocea against bacterial infection and further increase our understanding of IRFs function in innate immunity in teleosts.
['Tang|Jingteng|J|', 'Jiang|Lihua|L|', 'Liu|Wei|W|', 'Lou|Bao|B|', 'Wu|Changwen|C|', 'Zhang|Jianshe|J|']
D000818:Animals D003001:Cloning, Molecular D005393:Fish Diseases D029941:Fish Proteins D007113:Immunity, Innate D050835:Interferon Regulatory Factors D007668:Kidney D008099:Liver D016366:Open Reading Frames D010473:Perciformes D017385:Sequence Homology D013154:Spleen D014714:Vertebrates D014733:Vibrio D014735:Vibrio Infections
Larimichthys crocea IRF4/8/9 Innate immunity Gene expression