Presence of human papilloma virus in Caucasian women living in the central Europe diagnosed with vulvar intraepithelial neoplasia.
OBJECTIVES
The role of human papilloma virus (HPV) in the development of cancerous states of female reproductive tract has been widely debated. However, the information about presence of HPV in the Caucasian women living in the central Europe diagnosed with vulvar intraepithelial neoplasia (VIN) is missing. So far, no recommendation was made to complete HPV detection in time of vulvar biopsy or after the results of positive VIN are obtained. We aimed to assess the presence of HPV in women with vulvar intraepithelial neoplasia diagnosed at the Department of Gynecology, Obstetrics and Oncological Gynecology in Bytom, Poland.


MATERIAL AND METHODS
The retrospective examination of 120 consecutive vulvar biopsies obtained from women with persistent vulvar itching was done. Only patients with diagnosis of VIN were included in the further analysis. HPV DNA was detected using HPV Linear Array Genotyping Test including 14 HPV genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68).


RESULTS
Out of 120 vulvar samples retrieved, 18 women were positive for VIN, including15 usual VIN (uVIN) and three differentiated type (dVIN ). 10 samples were eligible for DNA detection. HPV DNA was found in two women with uVIN (HPV 16 and 51).


CONCLUSIONS
It is advisable to recommend HPV genotyping in women with VIN, regardless of their age and histologic type. The incidence of HPV infection in Caucasian women from the central Europe with VIN should be further studied.
['Robenek|Marta|M|', 'Olejek|Anita|A|', 'Witek|Lukasz|L|', 'Augusciak-Duma|Aleksandra|A|', 'Drozdzowska|Bogna|B|', 'Gabriel|Iwona|I|']
D000328:Adult D018290:Cervical Intraepithelial Neoplasia D005260:Female D006801:Humans D008875:Middle Aged D027383:Papillomaviridae D011044:Poland D012189:Retrospective Studies D012307:Risk Factors D002583:Uterine Cervical Neoplasms D014621:Vagina
human papilloma virus vulvar intraepithelial neoplasia vulvar itching