Quantitative Proteomics Analysis Confirmed Oxidative Metabolism Predominates in Streptomyces coelicolor versus Glycolytic Metabolism in Streptomyces lividans.
Recent physiological studies indicated that S. lividans metabolism was mainly glycolytic, whereas S. coelicolor metabolism was mainly oxidative. To determine whether such metabolic characteristics were correlated with consistent proteomics features, a comparative label-free, shotgun proteomics analysis of these strains was carried out. Among 2024 proteins identified, 360 showed significant differences in abundance between the strains. This study revealed that S. coelicolor catabolized glucose less actively than S. lividans, whereas the amino acids present in the medium were catabolized less actively by S. lividans than by S. coelicolor. The abundance of glycolytic proteins in S. lividans was consistent with its high glycolytic activity, whereas the abundance of proteins involved in the catabolism of amino acids in S. coelicolor provided an explanatory basis for its predominantly oxidative metabolism. In this study, conducted under conditions of low O2 availability, proteins involved in resistance to oxidative stress and those belonging to a DosR-like dormancy regulon were abundant in S. coelicolor, whereas tellurium resistance proteins were abundant in S. lividans. This indicated that the strains reacted differently to O2 limitation. Proteins belonging to the CDA, RED, and ACT pathways, usually highly expressed in S. coelicolor, were not detected under these conditions, whereas proteins of siderophores, 5-hydroxyectoine, and terpenoid biosynthetic pathways were present.
['Millan-Oropeza|Aaron|A|0000-0001-9740-8683', 'Henry|Céline|C|', 'Blein-Nicolas|Mélisande|M|', 'Aubert-Frambourg|Anne|A|', 'Moussa|Fathi|F|', 'Bleton|Jean|J|', 'Virolle|Marie-Jöelle|MJ|']
D000332:Aerobiosis D000596:Amino Acids D000693:Anaerobiosis D001426:Bacterial Proteins D020869:Gene Expression Profiling D015964:Gene Expression Regulation, Bacterial D005947:Glucose D006019:Glycolysis D058977:Molecular Sequence Annotation D010085:Oxidative Phosphorylation D010100:Oxygen D040901:Proteomics D018085:Regulon D013045:Species Specificity D048372:Streptomyces coelicolor D048170:Streptomyces lividans
oxidative metabolism Streptomyces glycolytic metabolism oxidative stress dormancy regulon tellurium resistance proteins mass spectrometry secondary metabolites shotgun label-free quantitative proteomics