Keratinocyte growth factor-2 inhibits bacterial infection with Pseudomonas aeruginosa pneumonia in a mouse model.
To determine protective effects of concurrent administration of Keratinocyte growth factor-2 (KGF-2) with Pseudomonas aeruginosa (P. aeruginosa) inoculation on the induced pneumonia. KGF-2 (5 mg/kg) was concurrently administered into the left lobe of 55 mice with P. aeruginosa PAO1 (5 × 10(6) CFU, half-lethal dose); 55 mice in the control group were concurrently administered PBS with the PAO1. We detected and analyzed: body temperature; amount of P. aeruginosa in homogenates; count of total number of nucleated cells and of mononuclear macrophages; protein concentration in bronchoalveolar lavage fluid (BALF); lung wet-to-dry weight ratio; cytokines in BALF and blood; and lung morphology. To study survival rate, concurrent administration of KGF-2 (experimental group) versus PBS (control) with a lethal dose of PAO1 (1 × 10(7) CFU was performed, and survivorship was documented for 7 days post-inoculation. The bacterial CFU in lung homogenates was significantly decreased in the KGF-2 group compared to the control group. There were significantly more mononuclear macrophages in the BALF from the KGF-2 group than from the control group (p < 0.05). KGF-2 increased the surfactant protein and GM-CSF mRNA in lung at 6 h and 72 h after inoculation. Significant reduction of lung injury scores, protein concentrations, lung wet-to-dry weight ratio, and IL-6 and TNF-α levels was noted in the KGF-2 treated rats at 72 h after inoculation (p < 0.05). The 7-day survival rate of the KGF-2 group was significantly higher than that of the control group (p < 0.05). Concurrent administration of KGF-2 facilitates the clearance of P. aeruginosa from the lungs, attenuates P. aeruginosa-induced lung injury, and extends the 7-day survival rate in mice model with P. aeruginosa pneumonia.
['Feng|Nana|N|', 'Wang|Qin|Q|', 'Zhou|Jian|J|', 'Li|Jing|J|', 'Wen|Xiaoxing|X|', 'Chen|Shujing|S|', 'Zhu|Zhenhua|Z|', 'Bai|Chunxue|C|', 'Song|Yuanlin|Y|', 'Li|Huayin|H|']
D000818:Animals D000900:Anti-Bacterial Agents D001992:Bronchoalveolar Lavage Fluid D051526:Fibroblast Growth Factor 10 D016178:Granulocyte-Macrophage Colony-Stimulating Factor D015850:Interleukin-6 D008168:Lung D051379:Mice D011552:Pseudomonas Infections D011550:Pseudomonas aeruginosa D051381:Rats D014409:Tumor Necrosis Factor-alpha
Keratinocyte growth factor-2 Pseudomonas aeruginosa Pneumonia Lung injury