Early detection of the growth of Mycobacterium tuberculosis using magnetophoretic immunoassay in liquid culture.
Tuberculosis (TB) is an often neglected, epidemic disease that remains to be controlled by contemporary techniques of medicine and biotechnology. In this study, a nanoscale sensing system, referred to as magnetophoretic immunoassay (MPI) was designed to capture culture filtrate protein (CFP)-10 antigens effectively using two different types of nanoparticles (NPs). Two specific monoclonal antibodies against CFP-10 antigen were used, including gold NPs for signaling and magnetic particles for separation. These results were carefully compared with those obtained using the commercial mycobacteria growth indicator tube (MGIT) test via 2 sequential clinical tests (with ca. 260 clinical samples). The sensing linearity of MPI was shown in the range of pico- to micromoles and the detection limit was 0.3pM. MPI using clinical samples shows robust and reliable sensing while monitoring Mycobacterium tuberculosis (MTB) growth with monitoring time 3-10 days) comparable to that with the MGIT test. Furthermore, MPI distinguished false-positive samples from MGIT-positive samples, probably containing non-tuberculous mycobacteria. Thus, MPI shows promise in early TB diagnosis.
['Kim|Jeonghyo|J|', 'Lee|Kil-Soo|KS|', 'Kim|Eun Bee|EB|', 'Paik|Seungwha|S|', 'Chang|Chulhun L|CL|', 'Park|Tae Jung|TJ|', 'Kim|Hwa-Jung|HJ|', 'Lee|Jaebeom|J|']
D000911:Antibodies, Monoclonal D000942:Antigens, Bacterial D001426:Bacterial Proteins D001431:Bacteriological Techniques D015374:Biosensing Techniques D052203:Ferrosoferric Oxide D006046:Gold D006801:Humans D007118:Immunoassay D057230:Limit of Detection D008280:Magnetics D053768:Metal Nanoparticles D009169:Mycobacterium tuberculosis D009170:Nontuberculous Mycobacteria D010316:Particle Size D013499:Surface Properties
Tuberculosis Magnetophoretic immunoassay CFP-10 antigen Magnetic particle Gold nanoparticle Magplas probes