Preparation, characterization, and optimization of altretamine-loaded solid lipid nanoparticles using Box-Behnken design and response surface methodology.
The objective of the present study was to prepare solid lipid nanoparticles (SLNs) of altretamine (ALT) by the hot homogenization and ultrasonication method. The study was conducted using the Box-Behnken design (BBD), with a 3(3) design and a total of 17 experimental runs, performed in combination with response surface methodology (RSM). The SLNs were evaluated for mean particle size, entrapment efficiency, and drug-loading. The optimized formulation, with a desirability factor of 0.92, was selected and characterized. In vitro release studies showed a biphasic release pattern from the SLNs for up to 24 h. The results of % EE (93.21 ± 1.5), %DL (1.15 ± 0.6), and mean diameter of (100.6 ± 2.1) nm, were very close to the predicted values.
['Gidwani|Bina|B|', 'Vyas|Amber|A|']
D006585:Altretamine D004337:Drug Carriers D065546:Drug Liberation D004355:Drug Stability D008055:Lipids D053758:Nanoparticles D010316:Particle Size D013501:Surface-Active Agents D000069453:Ultrasonic Waves
optimization solid lipid nanoparticles Box-Behnken design response surface methodology altretamine