Survey of ochratoxin A in rice from Iran using affinity column cleanup and HPLC with fluorescence detection.
This paper records the occurrence and levels of ochratoxin A (OTA) in rice using a HPLC technique preceded by an immunoaffinity clean-up step. The method was based on the extraction of finely ground rice sample with an acetonitrile/water (60 : 40, v/v) solution. Recovery was 98.9% while the limit of detection (LOD) was 0.2 ng g⁻¹. A total of 182 rice samples were analyzed with a frequency of contamination of 6%. Levels of OTA in positive samples ranged 0.2-4.8 ng g⁻¹, with an average contamination of all analyzed samples of 1.6 ng g⁻¹.
['Feizy|J|J|', 'Beheshti|H R|HR|', 'Fakoor Janati|S S|SS|', 'Khoshbakht Fahim|N|N|']
D053000:Analytic Sample Preparation Methods D002138:Calibration D002273:Carcinogens D002846:Chromatography, Affinity D002851:Chromatography, High Pressure Liquid D005506:Food Contamination D005513:Food Inspection D019983:Guideline Adherence D006291:Health Policy D006293:Health Promotion D006801:Humans D007492:Iran D057230:Limit of Detection D009793:Ochratoxins D012275:Oryza D015203:Reproducibility of Results D012639:Seeds D013050:Spectrometry, Fluorescence
ochratoxin A rice mycotoxins