Massilia buxea sp. nov., isolated from a rock surface.
A Gram-stain-negative, rod-shaped and motile bacterial strain, designated A9T, was isolated from the surface of rock collected from the shore of Nvshan lake in Mingguang, Anhui province, China. Phylogenetic analysis based on 16S rDNA sequence data showed that strain A9T was affiliated with the genus Massilia and showed the highest sequence similarities to Massilia plicata KCTC 12344T (98.8 %) and Massilia lurida CGMCC 1.10822T (97.9 %). The major fatty acids (>5 %) were summed feature 3 (C16 : 1ω7c and/or C15 : 0 iso 2-OH), C16 : 0 and C18 : 1ω7c. Strain A9T contained Q-8 as the predominant ubiquinone and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid as the predominant polar lipids. The DNA G+C content was 69.9 mol%. Mean DNA-DNA relatedness values between strain A9T and its closest phylogenetic relatives, M. plicata KCTC 12344T and M. lurida CGMCC 1.10822T, were 38.8 % and 23.23 %, respectively. On the basis of the results obtained in this study, strain A9T is considered to represent a novel species of the genus Massilia, for which the name Massilia buxea sp. nov. is proposed. The type strain is A9T (=DSM 103547T=CGMCC 1.15931T=KCTC 52429T).
['Sun|Le-Ni|LN|', 'Yang|En-Dong|ED|', 'Cui|Dan-Xi|DX|', 'Ni|Ye-Wen|YW|', 'Wang|Yu-Bo|YB|', 'Sun|Dong-Dong|DD|', 'Wang|Wei-Yun|WY|']
D015373:Bacterial Typing Techniques D001482:Base Composition D002681:China D004269:DNA, Bacterial D005227:Fatty Acids D009693:Nucleic Acid Hybridization D042802:Oxalobacteraceae D010743:Phospholipids D010802:Phylogeny D012336:RNA, Ribosomal, 16S D017422:Sequence Analysis, DNA D014451:Ubiquinone
Massilia buxea sp. nov