Immunophenotyping of Acute Myeloid Leukemia.
Immunophenotyping by multiparameter flow cytometry is a rapid and efficient technique to simultaneously assess and correlate multiple individual cell properties like size and internal complexity along with antigen expression in a population of cells. This method is utilized for rapid characterization of the blasts and classification of acute myeloid leukemia (AML), in both the peripheral blood (PB) and bone marrow (BM). This technique is not only useful in the initial diagnosis but also in monitoring and determining prognosis of the disease through minimal residual disease (MRD) testing. This chapter provides an overview of procedures for specimen processing, staining, and immunophenotyping of AML and describes the principles of data analysis for AML classification and MRD testing.
['Galera|Pallavi Kanwar|PK|', 'Jiang|Chunjie|C|', 'Braylan|Raul|R|']
D000941:Antigens D005434:Flow Cytometry D015972:Gene Expression Regulation, Neoplastic D006801:Humans D016130:Immunophenotyping D015470:Leukemia, Myeloid, Acute
Immunophenotype Acute myeloid leukemia Flow cytometry Minimal residual disease Myelodysplastic syndrome