Inhibition of UDP-glucuronosyltransferases (UGTs) by bromophenols (BPs).
Bromophenols (BPs) are important organic compounds which have become dominant pollutants during these years. Our present study investigated the potential inhibition behaviour of BPs on the activity of one of the most important phase II drug-metabolizing enzymes (DMEs), UDP-glucuronosyltransferases (UGTs). Recombinant UDP-glucuronosyltransferases (UGTs)-catalyzed glucuronidation of 4-methylumbelliferone (4-MU) was utilized as the probe reaction. 100 μM of BPs was utilized as the inhibition screening concentrations, and the complete inhibition profile of UGT isoforms by BPs was obtained. UGT1A7 was the most vulnerable UGT isoform towards BPs. Some structure-activity relationship for the inhibition of UGTs by BPs was found, and this relationship can be furtherly explained by the hydrophobic contacts of BPs with the activity cavity of UGTs using in silico docking method. The inhibition kinetics determination showed that the inhibition kinetic parameter Ki value was calculated to be 2.85, 3.99 and 31.00 μM for the inhibition of UGT1A3, UGT1A7, and UGT2B7 by representative BPs, 2,4,6-TBP. Combined with in vivo exposure concentration of 2,4,6-TBP, in vitro-in vivo extrapolation (IVIVE) was employed to demonstrate the moderate possibility for the inhibition of UGT1A3 and UGT1A7 by 2,4,6-TBP. In conclusion, our study gave the full description towards the inhibition of BPs towards UGT isoforms, which will provide a new perspective for elucidating the toxicity mechanism of bromophenols (BPs).
['Wang|Feige|F|', 'Wang|Shang|S|', 'Yang|Kai|K|', 'Liu|Yong-Zhe|YZ|', 'Yang|Kun|K|', 'Chen|Yao|Y|', 'Fang|Zhong-Ze|ZZ|']
D002384:Catalysis D014453:Glucuronosyltransferase D006801:Humans D006842:Hydrocarbons, Brominated D057927:Hydrophobic and Hydrophilic Interactions D007700:Kinetics D062105:Molecular Docking Simulation D010636:Phenols D020033:Protein Isoforms D013329:Structure-Activity Relationship
Inhibition Bromophenols (BPs) In vitro-in vivo extrapolation (IVIVE) Bromine substituent Human UDP-Glucuronosyltransferases (UGTs)