Ultrastructure changes induced by the phloroglucinol derivative agrimol G isolated from Leucosidea sericea in Haemonchus contortus.
Plant extracts used for the treatment of helminth infections in sheep are an alternative to chemical anthelmintic drugs. Previous studies have reported the anthelmintic activity of acetone leaf extracts of Leucosidea sericea. For this study, we evaluate the ultrastructure changes induced by the acetone leaf extract of L. sericea and the component agrimol G (AG) that was isolated for the first time on adult haemonchus parasites. Adult haemonchus parasites harvested from sheep were incubated with the plant extract and AG for 3 h and evaluated by both scanning and transmission electron microscopy in comparison and in combination with albendazole or ivermectin. In all cases the method of evaluation shows ultrastructural changes, with albendazole inducing mitochondrial damage and ivermectin inducing muscle degeneration, both as previously described. Incubation with the plant extract and AG resulted in the formation of numerous non-membrane bound multi-vesicular like bodies and evenly spread disruptions/erosion in the epicuticle. Combining AG with ivermectin or albendazole resulted in an absence of effect of AG. Based on the structural changes induced by AG, together with the absence of an effect in combination with ivermectin and albendazole would suggest a disrupted microtubular network. The latter does however require biochemical confirmation.
['Adamu|M|M|', 'Mukandiwa|L|L|', 'Awouafack|M D|MD|', 'Ahmed|A S|AS|', 'Eloff|J N|JN|', 'Naidoo|V|V|']
D000018:Abomasum D015766:Albendazole D000818:Animals D000871:Anthelmintics D004359:Drug Therapy, Combination D005404:Fixatives D005976:Glutaral D006188:Haemonchiasis D006189:Haemonchus D007559:Ivermectin D008855:Microscopy, Electron, Scanning D046529:Microscopy, Electron, Transmission D010696:Phloroglucinol D010936:Plant Extracts D027824:Rosaceae D012756:Sheep D012757:Sheep Diseases
Agrimol Leucosidea sericea Haemonchus Anthelmintic Electron microscopy