Effects of Mild Hypothermia on Cardiac and Neurological Function in Piglets Under Pathological and Physiological Stress Conditions.
To investigate the different effects of mild hypothermia on pathological and physiological stress conditions in piglets, 30 pigs were randomized into four groups: cardiac arrest and mild hypothermia (CA-MH group), cardiac arrest and normothermia (CA-NH group), non-CA-MH (NCA-MH group), and a sham operation. The same hypothermia intervention was implemented in both CA-MH and NCA-MH groups. The CA-NH group did not undergo therapeutic hypothermia after resuscitation. The hemodynamic parameters were recorded. Cerebral metabolism variables and neurotransmitters in the extracellular fluid were collected through microdialysis tubes. The serum of venous blood was used to detect levels of inflammatory factors. The cerebral function was evaluated. At 24 and 72 hours after resuscitation, the cerebral performance category and neurological deficit score in the CA-NH group had higher values. Heart rate and cardiac output (CO) in the CA-MH group during cooling were lower than that of the CA-NH group, but CO was higher after rewarming. Glucose was higher during cooling, and extracellular lactate and lactate/pyruvate ratio in the CA-MH group were lower than that of the CA-NH group. Noradrenaline and 5-hydroxytryptamine in the CA-MH and NCA-MH groups were lower than that of the CA-NH group and sham group during cooling, respectively. Inflammatory factor levels, including interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, and tumor necrosis factor-α, in the CA-MH group were lower than that of the CA-NH group at cooling for 12 hours. These values in the NCA-MH group were higher than that of the sham group. Under a light and an electron microscope, the worse pathological results of heart and brain were observed in the two cardiac arrest groups. Mild hypothermia can provide limited organ protection in the specific pathological condition caused by ischemia-reperfusion, but it may produce a negative effect in a normal physiological state.
['Yuan|Wei|W|', 'Wu|Jun-Yuan|JY|', 'Zhao|Yong-Zhen|YZ|', 'Li|Jie|J|', 'Li|Jie-Bin|JB|', 'Li|Zhen-Hua|ZH|', 'Li|Chun-Sheng|CS|']
D000818:Animals D000831:Animals, Newborn D015415:Biomarkers D001786:Blood Glucose D001833:Body Temperature Regulation D001921:Brain D001930:Brain Injuries D002560:Cerebrovascular Circulation D016207:Cytokines D004195:Disease Models, Animal D006323:Heart Arrest D006439:Hemodynamics D007036:Hypothermia, Induced D018836:Inflammation Mediators D008297:Male D009206:Myocardium D015427:Reperfusion Injury D034421:Sus scrofa D013997:Time Factors
mild hypothermia animal studies post-resuscitation cooling