Establishment of an in vitro model using the rat alveolar macrophage cell line NR8383.
OBJECTIVE
To establish an in vitro model of radiation-induced lung injury using rat lung alveolar macrophages (NR8383).


METHODS
Using a medical electronic linear accelerator, cells were irradiated with either 0 Gy or 6 Gy X-rays. At 6, 12, 24, 30 and 48 h, the DNA damage index (8-OHdG) and lipid damage index (MDA) were measured in the two groups. We also determined the levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and transforming growth factor-β (TGF-β).


RESULTS
The levels of 8-OHdG and MDA in the 6 Gy irradiation group were higher than those in the 0 Gy group at 6, 12, 24, 30 and 48 h after irradiation. The levels reached the highest value -6 h after irradiation, and then gradually decreased. The levels of the inflammatory factors TNF-α, TGF-β and IL-6 were higher in the 6 Gy irradiation group than those in the 0 Gy group at 6, 12, 24, 30 and 48 h after irradiation.


CONCLUSION
Six Gy X-ray irradiated NR8383 cells can be used to establish an in-vitro model of radiation-induced lung injury. The levels of 8-OHdG, MDA, TNF-α, TGF-β and IL-6 can be used as effective evaluation indicators.
['Wang|Jiawei|J|', 'Wang|Xuekai|X|', 'Sui|Yu|Y|', 'Zhang|Xieyu|X|', 'Hou|Wei|W|']
D000818:Animals D002460:Cell Line D006801:Humans D015850:Interleukin-6 D055370:Lung Injury D016676:Macrophages, Alveolar D008954:Models, Biological D009369:Neoplasms D011832:Radiation Injuries D011839:Radiation, Ionizing D011878:Radiotherapy D051381:Rats D014409:Tumor Necrosis Factor-alpha
Lung injury In vitro technique Macrophage, alveolar Radiation