Combined HPLC-DAD-MS, HPLC-MS(n) and NMR spectroscopy for quality control of plant extracts: the case of a commercial blend sold as dietary supplement.
The efficiency of 1D and 2D NMR spectroscopy along with HPLC-DAD-MS analyses in characterising the content of a dietary supplement is demonstrated. Experiments directly performed on a lyophilised sample of a commercial product gave details on the quality control of the product. The lack of the marker constituents of some of the declared plant species (Crataegus oxyacantha, Olea europea, Capsella bursa-pastoris and Fumaria officinalis) and the presence of banned adulterants, responsible for the strong antihypertensive effect of the supplement were established. The analyses proved the presence of indole alkaloids belonging to the group of Rauwolfia sp., such as ajmaline, reserpine and yohimbine. Quantitative HPLC analysis showed that the content of reserpine in the product was in the therapeutic range and therefore responsible for the collapses of the patients.
['Karioti|A|A|', 'Giocaliere|E|E|', 'Guccione|C|C|', 'Pieraccini|G|G|', 'Gallo|E|E|', 'Vannacci|A|A|', 'Bilia|A R|AR|']
D000470:Alkaloids D000959:Antihypertensive Agents D002138:Calibration D002626:Chemistry, Pharmaceutical D002851:Chromatography, High Pressure Liquid D019587:Dietary Supplements D004340:Drug Contamination D006801:Humans D009682:Magnetic Resonance Spectroscopy D013058:Mass Spectrometry D060735:Pharmacovigilance D010936:Plant Extracts D018515:Plant Leaves D010946:Plants, Medicinal D011786:Quality Control D011926:Rauwolfia D013678:Technology, Pharmaceutical
NMR Quality control Dietary supplements HPLC-UV–MS HPLC–MS(n) Phytovigilance