Sox13 is a novel early marker for hair follicle development.
Sox13, a group D member of the Sry-related high-mobility group box (Sox) transcription factor family, is expressed in various tissues including the hair follicle. However, its spatiotemporal expression patterns in the hair follicle and its role in hair development remain to be elucidated. To address these questions, we generated Sox13-LacZ-knock-in mice (Sox13LacZ/+), in which the LacZ reporter gene was inserted in-frame into exon 2, which contains the translation initiation codon. X-gal staining in Sox13LacZ/+ embryos revealed that Sox13 is initially expressed in the epithelial portion of the placode, and subsequently in the hair germ and the hair peg during early hair follicle development. In postnatal catagen and anagen, Sox13 was detected in the epithelial sheath, whereas in telogen, Sox13 was localized in the bulge region, where hair follicle stem cells reside. Immunohistochemistry with an anti-β-galactosidase antibody and anti-hair keratin antibodies that specifically mark the different layers of the hair follicle revealed that Sox13 was predominantly expressed in the outer root sheath in anagen. However, the integumentary structures of Sox13LacZ/LacZ mice were grossly and histologically indistinguishable from those of wild type mice. These results suggest that although Sox13 is dispensable for epidermal and adnexal development, Sox13 is a useful marker for early hair follicle development.
['Noto|Mai|M|', 'Noguchi|Natsuko|N|', 'Ishimura|Akihiko|A|', 'Kiyonari|Hiroshi|H|', 'Abe|Takaya|T|', 'Suzuki|Tomoko|T|', 'Hasunuma|Naoko|N|', 'Taira|Masanori|M|', 'Manabe|Motomu|M|', 'Osada|Shin-Ichi|SI|']
D000818:Animals D001324:Autoantigens D015415:Biomarkers D017630:Connexins D004622:Embryo, Mammalian D018507:Gene Expression Regulation, Developmental D018859:Hair Follicle D007150:Immunohistochemistry D051379:Mice D008822:Mice, Transgenic D062211:Spatio-Temporal Analysis D014157:Transcription Factors D029961:Zebrafish Proteins
Sox13 Hair follicle Development Transcription factor LacZ knock-in mouse