Multimodal Imaging Mass Spectrometry: Next Generation Molecular Mapping in Biology and Medicine.
Imaging mass spectrometry has become a mature molecular mapping technology that is used for molecular discovery in many medical and biological systems. While powerful by itself, imaging mass spectrometry can be complemented by the addition of other orthogonal, chemically informative imaging technologies to maximize the information gained from a single experiment and enable deeper understanding of biological processes. Within this review, we describe MALDI, SIMS, and DESI imaging mass spectrometric technologies and how these have been integrated with other analytical modalities such as microscopy, transcriptomics, spectroscopy, and electrochemistry in a field termed multimodal imaging. We explore the future of this field and discuss forthcoming developments that will bring new insights to help unravel the molecular complexities of biological systems, from single cells to functional tissue structures and organs.
['Neumann|Elizabeth K|EK|http://orcid.org/0000-0002-6078-3321', 'Djambazova|Katerina V|KV|', 'Caprioli|Richard M|RM|', 'Spraggins|Jeffrey M|JM|http://orcid.org/0000-0001-9198-5498']
D000818:Animals D055664:Electrochemical Techniques D020869:Gene Expression Profiling D006801:Humans D013058:Mass Spectrometry D008853:Microscopy D064847:Multimodal Imaging D013055:Spectrophotometry, Infrared D013059:Spectrum Analysis, Raman D059467:Transcriptome
imaging mass spectrometry chemical imaging multimodal analysis tissue analysis