Fetuin A is down-regulated in rats during heterotopic ossification after Achilles tenotomy.
We investigated the role of fetuin A during heterotopic ossification (HO) in rats following Achilles tenotomy. We performed a right midpoint Achilles tenotomy on 24 rats. At 5 and 10 h after surgery, we investigated the formation of ectopic bone using X-ray and histological examination. We evaluated the mRNA level of fetuin A using real-time PCR. Presence of fetuin A in the Achilles tendon was assessed by immunohistochemical staining. We also measured the serum concentration of fetuin A using enzyme linked immunosorbent assay (ELISA). The expression of fetuin A was significantly decreased in both the liver and Achilles tendon during HO. ELISA showed a small amount of fetuin A in blood throughout the development of HO. Immunohistochemical staining showed that fetuin A was abundant in the ectopic bone. Fetuin A appears to be involved in the formation of ectopic bone induced by Achilles tenotomy, and a deficiency of fetuin A plays a role in the development of HO.
['Wu|J|J|', 'Xue|F|F|', 'Shi|W Z|WZ|', 'Xiao|H J|HJ|']
D000125:Achilles Tendon D000818:Animals D004195:Disease Models, Animal D015536:Down-Regulation D004797:Enzyme-Linked Immunosorbent Assay D006801:Humans D007150:Immunohistochemistry D008297:Male D009999:Ossification, Heterotopic D016133:Polymerase Chain Reaction D012333:RNA, Messenger D051381:Rats D058971:Tenotomy D060749:alpha-2-HS-Glycoprotein
fetuin A rat heterotopic ossification