Arf6 Can Trigger Wave Regulatory Complex-Dependent Actin Assembly Independent of Arno.
The small GTPase ADP-ribosylation factor 6 (Arf6) anchors at the plasma membrane to orchestrate key functions, such as membrane trafficking and regulating cortical actin cytoskeleton rearrangement. A number of studies have identified key players that interact with Arf6 to regulate actin dynamics in diverse cell processes, yet it is still unknown whether Arf6 can directly signal to the wave regulatory complex to mediate actin assembly. By reconstituting actin dynamics on supported lipid bilayers, we found that Arf6 in co-ordination with Rac1(Ras-related C3 botulinum toxin substrate 1) can directly trigger actin polymerization by recruiting wave regulatory complex components. Interestingly, we demonstrated that Arf6 triggers actin assembly at the membrane directly without recruiting the Arf guanine nucleotide exchange factor (GEF) ARNO (ARF nucleotide-binding site opener), which is able to activate Arf1 to enable WRC-dependent actin assembly. Furthermore, using labelled E. coli, we demonstrated that actin assembly by Arf6 also contributes towards efficient phagocytosis in THP-1 macrophages. Taken together, this study reveals a mechanism for Arf6-driven actin polymerization.
['Singh|Vikash|V|', 'Davidson|Anthony C|AC|', 'Hume|Peter J|PJ|', 'Koronakis|Vassilis|V|']
D000089982:ADP-Ribosylation Factor 6 D020727:ADP-Ribosylation Factors D000199:Actins D020690:GTPase-Activating Proteins D006801:Humans D008264:Macrophages D008954:Models, Biological D010587:Phagocytosis D011485:Protein Binding D000074084:THP-1 Cells
actin cytoskeleton wave regulatory complex phagocytosis macrophages Arf GTPases host–pathogen interplay