Transcriptional Profiling of the Probiotic Escherichia coli Nissle 1917 Strain under Simulated Microgravity.
Long-term space missions affect the gut microbiome of astronauts, especially the viability of some pathogens. Probiotics may be an effective solution for the management of gut microbiomes, but there is a lack of studies regarding the physiology of probiotics in microgravity. Here, we investigated the effects of microgravity on the probiotic Escherichia coli Nissle 1917 (EcN) by comparing transcriptomic data during exponential and stationary growth phases under simulated microgravity and normal gravity. Microgravity conditions affected several physiological features of EcN, including its growth profile, biofilm formation, stress responses, metal ion transport/utilization, and response to carbon starvation. We found that some changes, such as decreased adhesion ability and acid resistance, may be disadvantageous to EcN relative to gut pathogens under microgravity, indicating the need to develop probiotics optimized for space flight.
['Yim|Jaewoo|J|', 'Cho|Sung Won|SW|', 'Kim|Beomhee|B|', 'Park|Sungwoo|S|0000-0001-6228-0288', 'Han|Yong Hee|YH|', 'Seo|Sang Woo|SW|0000-0002-7434-7053']
D002244:Carbon D019295:Computational Biology D004926:Escherichia coli D020869:Gene Expression Profiling D015964:Gene Expression Regulation, Bacterial D007473:Ion Channels D053858:Metabolic Networks and Pathways D008670:Metals D019936:Probiotics D013312:Stress, Physiological D059467:Transcriptome D014893:Weightlessness
probiotics microgravity E. coli Nissle 1917 RNA-sequencing