Ureolytic Prokaryotes in Soil: Community Abundance and Diversity.
Although the turnover of urea is a crucial process in nitrogen transformation in soil, limited information is currently available on the abundance and diversity of ureolytic prokaryotes. The abundance and diversity of the soil 16S rRNA gene and ureC (encoding a urease catalytic subunit) were examined in seven soil types using quantitative PCR and amplicon sequencing with Illumina MiSeq. The amplicon sequencing of ureC revealed that the ureolytic community was composed of phylogenetically varied prokaryotes, and we detected 363 to 1,685 species-level ureC operational taxonomic units (OTUs) per soil sample, whereas 5,984 OTUs were site-specific OTUs found in only one of the seven soil types.
['Oshiki|Mamoru|M|', 'Araki|Mitsuru|M|', 'Hirakata|Yuga|Y|', 'Hatamoto|Masashi|M|', 'Yamaguchi|Takashi|T|', 'Araki|Nobuo|N|']
D001419:Bacteria D044822:Biodiversity D004269:DNA, Bacterial D009584:Nitrogen D010802:Phylogeny D012336:RNA, Ribosomal, 16S D012987:Soil D012988:Soil Microbiology D014508:Urea D014510:Urease
ureolytic prokaryote ureC urease soil microbiology urea turnover