High expression of polo-like kinase 1 is associated with the metastasis and recurrence in urothelial carcinoma of bladder.
OBJECTIVES
Polo-like kinase 1 (Plk1) has been widely pursued as an oncology target because it is overexpressed in several human tumor types. To investigate whether Plk1 plays a general role in bladder urothelial carcinoma, we examined the expression of Plk1 protein in bladder urothelial carcinoma and cell lines, and analyzed the relationship among Plk1 protein expression, metastasis, and recurrence of urinary bladder urothelial carcinoma.


METHODS
Immunohistochemistry was used to detect the expression of Plk1 in 120 bladder urothelial carcinoma. Moreover, the expression of Plk1 was analyzed by Western blot in 60 bladder urothelial carcinoma and 21 normal epithelial tissues. MTT assay and flow cytometry and transwell assay were used to examine the proliferative and invasive ability of bladder cancer cells with the treatment of scytonemin (the inhibitor of Plk1). Statistical analysis was used to discuss the association between Plk1 expression and clinicopathologic parameters, tumor metastasis and recurrence, and the proliferative and invasive ability and cell cycle process of the bladder cancer cells.


RESULTS
There was a significantly higher Plk1expressions in bladder urothelial carcinoma and highly invasive bladder T24 cells than those in bladder normal tissues and the superficial bladder BIU-87 cells. Plk1 expression was positively correlated with histologic grade, pT stage, recurrence, and metastasis. With the increasing concentration of scytonemin, we found that not only the cell proliferation and invasion activity decreased significantly, but also the cell cycle was blocked at G2/M stage.


CONCLUSION
Plk1 expression status was closely correlated with important histopathologic characteristics (grades and stages) and the recurrence and metastasis of bladder urothelial carcinomas. Furthermore, Plk1 played an important function on the bladder cancer cells' proliferation by regulating the cancer cell cycle from G1/S to G2/M and probably promoted the invasion and metastasis of bladder cancer.
['Zhang|Zhe|Z|', 'Zhang|Guojun|G|', 'Kong|Chuize|C|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D015153:Blotting, Western D002295:Carcinoma, Transitional Cell D018797:Cell Cycle Proteins D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D005260:Female D059565:G2 Phase Cell Cycle Checkpoints D006801:Humans D007150:Immunohistochemistry D007211:Indoles D008297:Male D008875:Middle Aged D060787:Neoplasm Grading D009362:Neoplasm Metastasis D009364:Neoplasm Recurrence, Local D009367:Neoplasm Staging D010636:Phenols D017346:Protein Serine-Threonine Kinases D011518:Proto-Oncogene Proteins D001749:Urinary Bladder Neoplasms
Polo-like kinase 1 Bladder urothelial carcinoma Scytonemin Clinical stage Histopathologic grade