Primary Air-Liquid Interface Culture of Nasal Epithelium for Nasal Drug Delivery.
Nasal drug administration is a promising alternative to oral and parenteral administration for both local and systemic delivery of drugs. The benefits include its noninvasive nature, rapid absorption, and circumvention of first pass metabolism. Hence, the use of an in vitro model using human primary nasal epithelial cells could be key to understanding important functions and parameters of the respiratory epithelium. This model will enable investigators to address important and original research questions using a biologically relevant in vitro platform that mimics the in vivo nasal epithelial physiology. The purpose of this study was to establish, systematically characterize, and validate the use of a primary human nasal epithelium model cultured at the air-liquid interface for the study of inflammatory responses and drug transport and to simultaneously quantify drug effects on ciliary activity.
['Ong|Hui Xin|HX|', 'Jackson|Claire L|CL|', 'Cole|Janice L|JL|', 'Lackie|Peter M|PM|', 'Traini|Daniela|D|', 'Young|Paul M|PM|', 'Lucas|Jane|J|', 'Conway|Joy|J|']
D000281:Administration, Intranasal D000328:Adult D018929:Cell Culture Techniques D002478:Cells, Cultured D016503:Drug Delivery Systems D004847:Epithelial Cells D005260:Female D006801:Humans D008297:Male D008875:Middle Aged D009297:Nasal Mucosa D004364:Pharmaceutical Preparations D020545:Respiratory Mucosa
nasal drug delivery epithelial cells transport ciliary function tobramycin