Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells.
Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 µg mL-1 and 93.62 ± 3.53 µg mL-1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals.
['Fernando|Ilekuttige Priyan Shanura|IPS|0000-0002-9450-4239', 'Sanjeewa|Kalu Kapuge Asanka|KKA|0000-0001-7073-995X', 'Lee|Hyo Geun|HG|', 'Kim|Hyun-Soo|HS|', 'Vaas|Andaravaas Patabadige Jude Prasanna|APJP|', 'De Silva|Hondamuni Ireshika Chathurani|HIC|', 'Nanayakkara|Chandrika Malkanthi|CM|', 'Abeytunga|Dampegamage Thusitha Udayangani|DTU|', 'Lee|Dae-Sung|DS|', 'Lee|Jung-Suck|JS|', 'Jeon|You-Jin|YJ|']
D000970:Antineoplastic Agents D017209:Apoptosis D002453:Cell Cycle D045744:Cell Line, Tumor D049109:Cell Proliferation D004249:DNA Damage D018922:HL-60 Cells D006801:Humans D061986:MCF-7 Cells D008928:Mitochondria D011134:Polysaccharides D044647:Sargassum
fucoidan sargassum sulfated polysaccharide Celluclast Sri Lankan algae anticancer