Ischemia-Induced Multipotent Stem Cells Isolated from Stroke Patients Exhibit Higher Neurogenic Differentiation Potential than Bone Marrow-Derived Mesenchymal Stem Cells.
Perivascular areas of the brain harbor multipotent stem cells. We recently demonstrated that after a stroke, brain pericytes exhibit features of multipotent stem cells. Moreover, these ischemia-induced multipotent stem cells (iSCs) are present within ischemic areas of the brain of patients diagnosed with stroke. Although increasing evidence shows that iSCs have traits similar to those of mesenchymal stem cells (MSCs), the phenotypic similarities and differences between iSCs and MSCs remain unclear. In this study, we used iSCs extracted from stroke patients (h-iSCs) and compared their neurogenic potential with that of human MSCs (h-MSCs) in vitro. Microarray analysis, fluorescence-activated cell sorting, immunohistochemistry, and multielectrode array were performed to compare the characteristics of h-iSCs and h-MSCs. Although h-iSCs and h-MSCs had similar gene expression profiles, the percentage expressing the neural stem/progenitor cell marker nestin was significantly higher in h-iSCs than in h-MSCs. Consistent with these findings, h-iSCs, but not h-MSCs, differentiated into electrophysiologically functional neurons. In contrast, although both h-iSCs and h-MSCs were able to differentiate into several mesodermal lineages, including adipocytes, osteocytes, and chondrocytes, the potential of h-iSCs to differentiate into adipocytes and osteocytes was relatively low. These results suggest that compared with h-MSCs, h-iSCs predominantly exhibit neural rather than mesenchymal lineages. In addition, these results indicate that h-iSCs have the potential to repair the injured brain of patients with stroke by directly differentiating into neuronal lineages.
['Rajbhandari|Saujanya|S|', 'Beppu|Mikiya|M|', 'Takagi|Toshinori|T|', 'Nakano-Doi|Akiko|A|', 'Nakagomi|Nami|N|', 'Matsuyama|Tomohiro|T|', 'Nakagomi|Takayuki|T|', 'Yoshimura|Shinichi|S|']
D000368:Aged D000369:Aged, 80 and over D002545:Brain Ischemia D002454:Cell Differentiation D002469:Cell Separation D020219:Chondrogenesis D055724:Electrophysiological Phenomena D005260:Female D006801:Humans D008297:Male D059630:Mesenchymal Stem Cells D008648:Mesoderm D039902:Multipotent Stem Cells D055495:Neurogenesis D009474:Neurons D020521:Stroke
multipotent stem cells stroke mesenchymal stem cells brain pericytes ischemia