Molecular Cloning and Characterization of SYCP3 and TSEG2 Genes in the Testicles of Sexually Mature and Immature Yak.
Testis-specific genes play an essential part in the centromere union during meiosis in male germ cells, spermatogenesis, and in fertility. Previously, there was no research report available on the expression pattern of SYCP3 and TSEG2 genes in different ages of yaks. Therefore, the current research compared the expression profiling of SYCP3 and TSEG2 genes in testes of yaks. The expression pattern of SYCP3 and TSEG2 mRNA was investigated using qPCR, semi-quantitative PCR, western blot, immunohistochemistry, and molecular bioinformatics. Our findings displayed that SYCP3 and TSEG2 genes were prominently expressed in the testicles of yaks as compared to other organs. On the other hand, the protein encoded by yak SYCP3 contains Cor1/Xlr/Xmr conserved regions, while the protein encoded by yak TSEG2 contains synaptonemal complex central element protein 3. Additionally, multiple alignments sequences indicated that proteins encoded by Datong yak SYCP3 and TSEG2 were highly conserved among mammals. Moreover, western blot analysis specified that the molecular mass of SYCP3 protein was 34-kDa and TSEG2 protein 90-kDa in the yak. Furthermore, the results of immunohistochemistry also revealed the prominent expression of these proteins in the testis of mature yaks, which indicated that SYCP3 and TSEG2 might be essential for spermatogenesis, induction of central element assembly, and homologous recombination.
['Kalwar|Qudratullah|Q|', 'Chu|Min|M|', 'Ahmad|Anum Ali|AA|', 'Ma|Xiaoming|X|', 'Zhang|Renzheng|R|', 'Ma|Fulong|F|', 'Xie|Jianpeng|J|', 'Ding|Xuezhi|X|', 'Wu|Xiaoyun|X|', 'Bao|Pengjia|P|', 'Yan|Ping|P|']
D000818:Animals D002417:Cattle D003001:Cloning, Molecular D005854:Germ Cells D008297:Male D008540:Meiosis D009687:Nuclear Proteins D012333:RNA, Messenger D012741:Sexual Maturation D013091:Spermatogenesis D013573:Synaptonemal Complex D013737:Testis
cloning SYCP3 TSEG2 Yak spermatogenesis gene expression