Effect of microRNA-145 on proliferation and apoptosis of human non-small cell lung cancer A549 cells by regulating mTOR signaling pathway.
Our study aims to explore the impact of microRNA-145 (miR-145) on proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells by modulating mTOR signaling pathway. Paired NSCLC and adjacent normal tissues were obtained from 50 NSCLC patients between January 2014 and January 2016. Human lung adenocarcinoma A549 cell line was assigned into the control, mimics, mimics-negative control (NC), inhibitor and inhibitor-NC groups. Quantitative RT-PCR and Western blot were applied to detect the expressions of miR-145 and mTOR. MTT, scratch test, Transwell and flow cytometry were carried out to analyze the effect of miR-145 on A549 cell proliferation, migration, invasion, apoptosis and cycle distribution and regulation of mTOR signaling pathway. MiR-145 expression in NSCLC tissues was lower than that in adjacent normal tissues, while mTOR expression was higher in NSCLC tissues than in adjacent normal tissues. Compared with the control and mimics groups, cell proliferation, migration and invasion abilities decreased in the mimics group; additionally, cell apoptosis rate increased and cells were mostly in G1 phase. MiR-145 inhibited the activation of mTOR signaling pathway. Compared with the control and inhibitor-NC groups, mTOR signaling pathway was activated and cell proliferation, migration and invasion abilities increased in the inhibitor group, and cell apoptosis rate decreased and cells were less in G1 phase. The study indicated that miR-145 expression was low in NSCLC and influenced NSCLC cells by negatively regulating mTOR signaling pathway. This article is protected by copyright. All rights reserved.
['Li|Jian-Cheng|JC|', 'Zheng|Jun-Qiong|JQ|']
MicroRNA-145 Non-small cell lung cancer mTOR Cell proliferation Cell apoptosis