Comparison of Drug Inhibitory Effects ([Formula: see text]) in Monolayer and Spheroid Cultures.
Traditionally, the monolayer (two-dimensional) cell cultures are used for initial evaluation of the effectiveness of anticancer drugs. In particular, these experiments provide the [Formula: see text] curves that determine drug concentration that can inhibit growth of a tumor colony by half when compared to the cells grown with no exposure to the drug. Low [Formula: see text] value means that the drug is effective at low concentrations, and thus will show lower systemic toxicity when administered to the patient. However, in these experiments cells are grown in a monolayer, all well exposed to the drug, while in vivo tumors expand as three-dimensional multicellular masses, where inner cells have a limited access to the drug. Therefore, we performed computational studies to compare the [Formula: see text] curves for cells grown as a two-dimensional monolayer and a cross section through a three-dimensional spheroid. Our results identified conditions (drug diffusivity, drug action mechanisms and cell proliferation capabilities) under which these [Formula: see text] curves differ significantly. This will help experimentalists to better determine drug dosage for future in vivo experiments and clinical trials.
['Berrouet|Catherine|C|', 'Dorilas|Naika|N|', 'Rejniak|Katarzyna A|KA|0000-0003-2093-2422', 'Tuncer|Necibe|N|0000-0003-3168-4110']
D000970:Antineoplastic Agents D049109:Cell Proliferation D002470:Cell Survival D019295:Computational Biology D003198:Computer Simulation D004305:Dose-Response Relationship, Drug D004354:Drug Screening Assays, Antitumor D006801:Humans D055641:Mathematical Concepts D008938:Mitosis D008954:Models, Biological D009369:Neoplasms D018874:Spheroids, Cellular D014407:Tumor Cells, Cultured
values Drugs efficacy Monolayer versus spheroid cell cultures