Synthesis and characterization of biotin modified cholesteryl pullulan as a novel anticancer drug carrier.
A series of biotin modified cholesteryl pullulan (Bio-CHSP) conjugates with different degrees of substitution (DS) of biotin moiety were synthesized and characterized by Fourier transform infrared (FT-IR), proton nuclear magnetic resonance ((1)H NMR) and X-ray diffraction (XRD). Bio-CHSP conjugates were amphiphilic in nature and their self-aggregation behavior in aqueous media was evaluated by the fluorescence probe technique. Bio-CHSP self-aggregated nanoparticles (Bio-CHSP NPs) were prepared and analyzed by dynamic light scattering (DLS), zeta potential and transmission electron microscopy (TEM) technologies. These novel nanoparticles were almost spherical in shape, and their size, ranging from 178.8 to 100.0 nm. The safety of Bio-CHSP NPs was studied through single dose toxicity test in mice, and the result showed that Bio-CHSP NPs were well tolerated at the intravenous dose of 200 mg/kg in mice. Moreover, as a model anticancer drug, mitoxantrone loaded Bio-CHSP NPs were also prepared and characterized in this study.
['Yang|Wenzhi|W|', 'Wang|Miaomiao|M|', 'Ma|Lilan|L|', 'Li|Haiying|H|', 'Huang|Le|L|']
D000818:Animals D000970:Antineoplastic Agents D001710:Biotin D002784:Cholesterol D004337:Drug Carriers D004339:Drug Compounding D005260:Female D005936:Glucans D006001:Glycoconjugates D007700:Kinetics D009682:Magnetic Resonance Spectroscopy D008297:Male D051379:Mice D008813:Mice, Inbred ICR D008942:Mitoxantrone D015394:Molecular Structure D053758:Nanoparticles D010316:Particle Size D017550:Spectroscopy, Fourier Transform Infrared D014961:X-Ray Diffraction
Self-aggregated nanoparticle Mitoxantrone Biotin-modified cholesteryl pullulan Drug delivery