Toscana virus genome stability: data from a meningoencephalitis case in Mantua, Italy.
In July of 2013, samples from a patient with a neurological syndrome were collected from Mantua hospital and sent to the National Reference Laboratory for Arboviruses (National Institute of Health, Rome). On the basis of the symptoms, serological and molecular assays were performed to diagnose either West Nile virus (WNV) or Toscana virus (TOSV) infection. Molecular and serological tests confirmed TOSV infection. Virus isolation was obtained from cerebrospinal fluid. A full genome sequence was determined from this TOSV strain with next-generation sequencing using Ion Torrent technology. Nucleotide and amino acidic sequences grouped phylogenetically with lineage TOSV A and showed a low genome variability.
['Magurano|Fabio|F|', 'Baggieri|Melissa|M|', 'Gattuso|Gianni|G|', 'Fortuna|Claudia|C|', 'Remoli|Maria Elena|ME|', 'Vaccari|Gabriele|G|', 'Zaccaria|Guendalina|G|', 'Marchi|Antonella|A|', 'Bucci|Paola|P|', 'Benedetti|Eleonora|E|', 'Fiorentini|Cristiano|C|', 'Nicoletti|Loredana|L|']
D000328:Adult D016679:Genome, Viral D042822:Genomic Instability D006801:Humans D007558:Italy D008297:Male D008590:Meningoencephalitis D008969:Molecular Sequence Data D010217:Phlebotomus Fever D012367:RNA, Viral D029301:Sandfly fever Naples virus D017423:Sequence Analysis, RNA
Toscana virus Isolation Next-generation sequencing Phlebovirus Sandfly