Characterization and utility of phages bearing peptides with affinity to porcine reproductive and respiratory syndrome virus nsp7 protein.
High-affinity peptides to porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein (nsp) 7 were identified using phage-display technology. Five 12-amino-acid peptide sequences were identified after six rounds of biopanning. A putative CD##WC motif was found in two different consensus peptides borne by phages 4 and 5. The peptides borne by phages 4, 5, and 6 were synthesized for subsequent experiments, according to the results of the binding assays. Immunofluorescence assay revealed that all these peptides recognized nsp7 in PRRSV-infected cells. Furthermore, the peptides demonstrated antiviral activities, with peptides 5 and 6 showing effective inhibition. Early peptide stimulation was associated with strong antiviral activity, and the inhibitory effects of the peptides were dose-dependent at 36 and 48 h post-infection. Peptide 5 was selected to detect the intracellular localization of nsp7 by confocal microscopy. This peptide had a similar effect to anti-nsp7 monoclonal antibody on nsp7. These results suggest that high-affinity peptides to PRRSV nsp7 could mimic the potential of nsp7 antibody as a diagnostic reagent for virus detection. Moreover, the peptides selected in this study represented a potentially effective antiviral candidate to inhibit PRRSV.
['Wang|Heng|H|', 'Liu|Rongchang|R|', 'Cui|Jin|J|', 'Deng|Shengchao|S|', 'Xie|Jiexiong|J|', 'Nin|Zhangyong|Z|', 'Zhang|Guihong|G|']
D000818:Animals D000998:Antiviral Agents D003955:Diagnostic Tests, Routine D004358:Drug Therapy D007202:Indicators and Reagents D008403:Mass Screening D019151:Peptide Library D010455:Peptides D019318:Porcine Reproductive and Respiratory Syndrome D019316:Porcine respiratory and reproductive syndrome virus D011485:Protein Binding D013552:Swine D017361:Viral Nonstructural Proteins
Peptide nsp7 protein PRRSV Antiviral activity Phage display