Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA.
Somatic reprogramming to generate induced pluripotent stem cells, or iPSC, is a powerful tool in developmental biology, disease modeling, and regenerative medicine. microRNAs have been shown to regulate many key pathways in iPSC induction. Here we describe a microRNA mimic enhanced somatic reprogramming process starting from mouse embryonic fibroblast isolation to iPSC induction to colony derivation and characterization.
['Dang|Jason|J|', 'Rana|Tariq M|TM|']
D000818:Animals D018929:Cell Culture Techniques D002478:Cells, Cultured D065150:Cellular Reprogramming D000067470:Cellular Reprogramming Techniques D058732:Embryoid Bodies D005260:Female D005347:Fibroblasts D057026:Induced Pluripotent Stem Cells D051379:Mice D008819:Mice, Nude D035683:MicroRNAs D013724:Teratoma
Induced pluripotent stem cell MicroRNA Somatic reprogramming Mouse embryonic fibroblasts Differentiation Embryoid body Teratoma Transfection