Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks.
In this present work, we developed a phenol grafted polyglucuronic acid (PGU) and investigated the usefulness in tissue engineering field by using this derivative as a bioink component allowing gelation in extrusion-based 3D bioprinting. The PGU derivative was obtained by conjugating with tyramine, and the aqueous solution of the derivative was curable through a horseradish peroxidase (HRP)-catalyzed reaction. From 2.0 w/v% solution of the derivative containing 5 U/mL HRP, hydrogel constructs were successfully obtained with a good shape fidelity to blueprints. Mouse fibroblasts and human hepatoma cells enclosed in the printed constructs showed about 95% viability the day after printing and survived for 11 days of study without a remarkable decrease in viability. These results demonstrate the great potential of the PGU derivative in tissue engineering field especially as an ink component of extrusion-based 3D bioprinting.
['Sakai|Shinji|S|', 'Kotani|Takashi|T|', 'Harada|Ryohei|R|', 'Goto|Ryota|R|', 'Morita|Takahiro|T|', 'Bouissil|Soukaina|S|', 'Dubessay|Pascal|P|', 'Pierre|Guillaume|G|', 'Michaud|Philippe|P|', 'El Boutachfaiti|Redouan|R|', 'Nakahata|Masaki|M|', 'Kojima|Masaru|M|', 'Petit|Emmanuel|E|', 'Delattre|Cédric|C|']
Polyglucuronic acid Bioprinting Tissue engineering Horseradish peroxidase 3D-printing