Xanthan gum and Locust bean gum gel supports in vitro development of porcine oocytes derived from early antral follicles.
Early antral follicle (EAF)-derived porcine oocytes develop more readily on polyacrylamide-gel (PAG) than on plastic plates. Xanthan gum (XG) and locust bean gum (LBG) are edible polysaccharides. We investigated XG-LBG gel supports in the development of EAF-derived porcine oocytes. XG and LBG were mixed in a 1:1 ratio to form a substrate. We cultured oocyte granulosa cell complexes (OGCs) from the EAFs on XG-LBG gels of various concentrations. The oocyte diameters were comparable among the 0.3, 0.5, and 1.0% gels; granulosa cell proliferation was greater on the 1.0% gel. The proliferation and survival rates of the granulosa cells, and the histone H4 at lysine 12 acetylation levels were higher in OGCs cultured on 1.0% XG-LBG than those grown on 0.3% PAG. Development to the blastocyst stage was 13.8% for the XG-LBG gels and 9.4% for PAG. In conclusion, XG-LBG are safe and efficient substrates for in vitro culture of oocytes.
['Munakata|Yasuhisa|Y|', 'Sugimoto|Ayaka|A|', 'Shirasuna|Koumei|K|', 'Kuwayama|Takehito|T|', 'Iwata|Hisataka|H|']
D000818:Animals D018929:Cell Culture Techniques D002469:Cell Separation D002478:Cells, Cultured D005260:Female D005685:Galactans D005782:Gels D006107:Granulosa Cells D059471:In Vitro Oocyte Maturation Techniques D008351:Mannans D009865:Oocytes D009866:Oogenesis D006080:Ovarian Follicle D053149:Plant Gums D011135:Polysaccharides, Bacterial D013552:Swine
Xanthan gum Locust bean gum Oocyte Early antral follicle Pig