Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia.
An immunochromatographic test system has been developed for the simultaneous rapid multiplex serodiagnostics of bovine brucellosis, tuberculosis, and leukemia. The test system is based on the use of a conjugate of gold nanoparticles with the chimeric protein Cysteine-A/G and three analytical zones with immobilized pathogen antigens: Brucella abortus lipolysaccharide, recombinant proteins MPB64 and MPB83-MPB63 of Mycobacterium bovis, and recombinant protein p24 of the bovine leukemia virus. Prototypes of the test system were tested on 98 samples of sera from healthy and infected animals. The diagnostic sensitivity of the developed test system was 92% for brucellosis, 92% for tuberculosis, and 96% for leukemia. False positive test results were not observed.
['Barshevskaya|Lyubov V|LV|', 'Sotnikov|Dmitriy V|DV|', 'Zherdev|Anatoly V|AV|0000-0003-3008-2839', 'Khassenov|Bekbolat B|BB|', 'Baltin|Kayrat K|KK|', 'Eskendirova|Saule Z|SZ|', 'Mukanov|Kassym K|KK|', 'Mukantayev|Kanatbek K|KK|', 'Dzantiev|Boris B|BB|0000-0003-4008-4918']
D000818:Animals D002007:Brucellosis, Bovine D002417:Cattle D016583:Enzootic Bovine Leukosis D004797:Enzyme-Linked Immunosorbent Assay D006046:Gold D007118:Immunoassay D053768:Metal Nanoparticles D012698:Serologic Tests D044967:Serum D014380:Tuberculosis, Bovine
serodiagnosis bovine brucellosis tuberculosis leukemia immunochromatographic assay