Reference values for acetyl and butyrylcholinesterases in cattle under actual management conditions, hepatic and renal function by application of chlorpyrifos.
Chlorpyrifos is an anticholinesterase organophosphate insecticide widely used in Argentina in the production of food derived from animal, fruit and horticultural origin and is reported as a residue within these products. Local reference values for acetyl and butyrylcholinesterase were determined in Aberdeen Angus bovine and cross bred cattle (n = 25), a requirement to be able to evaluate toxicity of commercial organophosphate and carbamate formulations. The activity of cholinesterase enzymes presented an overall mean of 2,183.00 ± 485.6 IU L-1 for erythrocyte acetylcholinesterase and 203.1 ± 42.06 IU L-1 for plasma butyrylcholinesterase, which are used as reference values for meat steers within a system of intensive production in a semi-arid region. The toxic potential of chlorpyrifos in steers of the same breeds (n = 12) was assessed applying chlorpyrifos 15.00% Tipertox® in a single therapeutic dose of 7.50 mg kg-1 by topical route. Prior to application and then on day 1 and day 21 post-application, both blood cholinesterases, serum chlorpyrifos concentration by ultra-high resolution liquid chromatography with mass detector, analysis of blood counts, total proteins, liver enzymes, urea and creatinine were evaluated. The mean plasma concentration of chlorpyrifos was 27.90 ug L-1 at 24 h. The findings indicate that the therapeutic treatment of castrated male bovines treated with chlorpyrifos, applied by pour-on according to the manufacturer's instructions, does not cause changes in the variables evaluated.
['Ferré|Daniela M|DM|', 'Lentini|Valeria R|VR|', 'Romano|R Raquel|RR|', 'Ludueña|Hector R|HR|', 'Jotallán|Paola J|PJ|', 'Gorla|Nora B M|NBM|']
D000110:Acetylcholinesterase D000818:Animals D001118:Argentina D002091:Butyrylcholinesterase D002417:Cattle D004390:Chlorpyrifos D002800:Cholinesterase Inhibitors D002851:Chromatography, High Pressure Liquid D004912:Erythrocytes D005506:Food Contamination D007306:Insecticides D007668:Kidney D008099:Liver D008297:Male D010573:Pesticide Residues D012016:Reference Values
cattle Chlorpyrifos UHPLC chlolinesterases hematology renal and liver function