Mosquito microbiota cluster by host sampling location.
BACKGROUND
Microbial communities that inhabit the mosquito body play an import role in host biology and may have potential for mosquito control. However, the forces that shape these microbial communities are poorly understood.


METHODS
To gain a better understanding of how host location influences the composition and diversity of mosquito microbiota, we performed a survey of microbial communities in mosquito samples collected from six USA states using HiSeq sequencing of the 16S rRNA gene.


RESULTS
A total of 284 bacterial operational taxonomic units (OTUs) belonging to 14 phyla were detected in nine mosquito species, with Proteobacteria, Firmicutes and Actinobacteria accounting for 95% of total sequences. OTU richness varied markedly within and between mosquito species. The microbial composition and diversity was heavily influenced by the site of mosquito collection, suggesting that host location plays an important role in shaping the mosquito microbiota.


CONCLUSIONS
Variation in microbial composition and diversity between mosquitoes from different locations may have important implications on vector competence and transmission dynamics of mosquito-borne pathogens. Future studies should investigate the environmental factors responsible for these variations and the role of key bacteria characterized in this study on mosquito biology and their potential application in symbiotic control of mosquito-borne diseases.
['Muturi|Ephantus J|EJ|', 'Lagos-Kutz|Doris|D|', 'Dunlap|Christopher|C|', 'Ramirez|Jose L|JL|', 'Rooney|Alejandro P|AP|', 'Hartman|Glen L|GL|', 'Fields|Christopher J|CJ|', 'Rendon|Gloria|G|', 'Kim|Chang-Hyun|CH|']
D039903:Actinobacteria D000818:Animals D001419:Bacteria D044822:Biodiversity D004269:DNA, Bacterial D004777:Environment D000068536:Firmicutes D059014:High-Throughput Nucleotide Sequencing D064307:Microbiota D009032:Mosquito Control D000072138:Mosquito Vectors D020560:Proteobacteria D012336:RNA, Ribosomal, 16S D014481:United States
Mosquitoes Microbiota HiSeq 16S rRNA gene