rAAV-mediated delivery of brain-derived neurotrophic factor promotes neurite outgrowth and protects neurodegeneration in focal ischemic model.
Stroke is one of the neurological diseases which lead to permanently neuronal damage after temporary or long-term occlusion of vessels or after heart attack. However, there are few efficient strategies to prevent or treat this kind of insult in clinical because the consequence is irreversible and could be long-lasting after the onset of stroke. Gene therapy especially using viral system has long been addressed to be of great potential to reduce the damage. Here, we generated recombinant adeno-associated virus (rAAV) carrying brain-derived neurotrophic factor (BDNF) gene. Cells infected with rAAV-BDNF could be able to produce functional BDNF which promoted neurite outgrowth and protected neurons from apoptosis induced by serum deprivation. Further more, single injection of rAAV showed neuroprotection against cell death in focal ischemic model. These results showed that rAAV-mediated gene delivery is functional, which shed light to the future application of viral system-based gene therapy in clinical.
['Zhang|Jingyu|J|', 'Yu|Zhigang|Z|', 'Yu|Zhiqiang|Z|', 'Yang|Zichao|Z|', 'Zhao|Hong|H|', 'Liu|Luran|L|', 'Zhao|Jiexu|J|']
D000818:Animals D017209:Apoptosis D002545:Brain Ischemia D019208:Brain-Derived Neurotrophic Factor D002470:Cell Survival D002478:Cells, Cultured D018076:DNA, Complementary D000229:Dependovirus D004195:Disease Models, Animal D015870:Gene Expression D018014:Gene Transfer Techniques D015316:Genetic Therapy D005822:Genetic Vectors D006801:Humans D008297:Male D009410:Nerve Degeneration D016501:Neurites D018696:Neuroprotective Agents D051381:Rats D017207:Rats, Sprague-Dawley D019076:Transgenes
brain-derived neurotrophic factor Stroke gene therapy recombinant adeno-associated virus