Modified expression of vitamin D receptor and CYP27B1 in denervation-induced muscle atrophy.
The vitamin D pathway is related to the mass and function of skeletal muscles. Several studies have demonstrated the role of vitamin D receptor (VDR) and CYP27B1 in skeletal muscles, suggesting that these proteins may regulate skeletal muscles and their function. However, it remains unclear whether the expression of VDR and CYP27B1 is modified in skeletal muscle atrophy. We investigated whether denervation-induced muscle atrophy is associated with altered expression of VDR and CYP27B1 in murine skeletal muscles. Skeletal muscles were excised from C57BL/6J mice, 3 and 7 days after the mice underwent denervation surgery. Denervation induced muscle atrophy and enhanced the expression of MuRF1 and Atrogin-1 in the gastrocnemius and soleus. The protein expression of VDR was increased in the denervated gastrocnemius; in contrast, denervation decreased the protein expression of CYP27B1 in the gastrocnemius and soleus. These results suggest that denervation-induced muscle atrophy is associated with changes in the expression of vitamin D-related proteins in murine skeletal muscles.
['Mori|Risako|R|', 'Yokokawa|Takumi|T|', 'Fujita|Satoshi|S|']
D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase D000818:Animals D004195:Disease Models, Animal D008297:Male D008810:Mice, Inbred C57BL D009121:Muscle Denervation D009133:Muscular Atrophy D046988:Proteasome Endopeptidase Complex D018167:Receptors, Calcitriol D015398:Signal Transduction D025801:Ubiquitin
Vitamin D receptor CYP27B1 Muscle atrophy Skeletal muscle Denervation