Real-Time PCR Targeting the penA Mosaic XXXIV Type for Prediction of Extended-Spectrum-Cephalosporin Susceptibility in Clinical Neisseria gonorrhoeae Isolates.
Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum cephalosporins (ESCs) are increasing. We developed an assay to predict N. gonorrhoeae susceptibility to ESCs by targeting penA mosaic XXXIV, an allele prevalent among U.S. isolates with elevated ESC MICs. The assay was 97% sensitive and 100% specific for predicting at least one ESC MIC above the CDC alert value among clinical isolates, and it could be multiplexed with a previously validated gyrA PCR to predict ciprofloxacin susceptibility.
['Wong|L K|LK|', 'Hemarajata|P|P|0000-0001-6182-9136', 'Soge|O O|OO|0000-0002-8504-2319', 'Humphries|R M|RM|', 'Klausner|J D|JD|']
D000483:Alleles D000900:Anti-Bacterial Agents D001426:Bacterial Proteins D018550:Cephalosporin Resistance D002511:Cephalosporins D027081:DNA Gyrase D006069:Gonorrhea D006801:Humans D057230:Limit of Detection D008826:Microbial Sensitivity Tests D009030:Mosaicism D009344:Neisseria gonorrhoeae D060888:Real-Time Polymerase Chain Reaction D012680:Sensitivity and Specificity D014481:United States
Neisseria gonorrhoeae extended-spectrum cephalosporins cephalosporin antibiotic resistance antimicrobial resistance ceftriaxone gonorrhea molecular methods