Analysis of trace microcystins in vegetables using solid-phase extraction followed by high performance liquid chromatography triple-quadrupole mass spectrometry.
A selective and sensitive method for the simultaneous detection of three common and hazardous microcystins (microcystins-LR, -RR, and -YR) in various vegetables was established using solid-phase extraction followed by high performance liquid chromatography coupled with mass spectrometry. The methanol-water proportion ratio of the extraction solvent and its acidity, as well as the efficiencies of solid-phase extraction, were evaluated to optimize a pretreatment procedure for extracting the microcystins from 10 vegetable matrices. The limits of detection and quantitation were below 7.5 μg/kg (dw) and 25 μg/kg (dw), respectively, in different vegetable matrices. The recoveries of the microcystins in the 10 vegetable matrices ranged from 61.3 to 117.3%, with RSDs of 0.2-18.3%. The established method was used to analyze 28 field vegetable samples collected from the sides of Lake Dianchi, and microcystin-RR was found in almost all samples at concentrations of 36.4-2352.2 μg/kg (dw).
['Li|Yan-Wen|YW|', 'Zhan|Xiao-Jing|XJ|', 'Xiang|Lei|L|', 'Deng|Zhe-Shen|ZS|', 'Huang|Bin-Hui|BH|', 'Wen|Hong-Fei|HF|', 'Sun|Teng-Fei|TF|', 'Cai|Quan-Ying|QY|', 'Li|Hui|H|', 'Mo|Ce-Hui|CH|']
D002851:Chromatography, High Pressure Liquid D005506:Food Contamination D052998:Microcystins D052616:Solid Phase Extraction D053719:Tandem Mass Spectrometry D014675:Vegetables
microcystins vegetable HPLC-MS/MS matrix effect simultaneous analysis