Inhibiting adhesion events by Panax notoginseng saponins and Ginsenoside Rb1 protecting arteries via activation of Nrf2 and suppression of p38 - VCAM-1 signal pathway.
ETHNOPHARMACOLOGICAL RELEVANCE
Asian countries, such as China, Japan, and Korea, have witnessed a history of more than 1000 years of Panax notoginseng (Burk.) F.H. Chen's application as a famous traditional medicine for cardiovascular diseases (Zhou et al., 2004). The use of Panax notoginseng (Sanqi) was first recorded in "Bencao Gangmu", which was written by Li Shizhen, a Chinese pharmacologist of the MING dynasty, in 1578. It is included in "The Plant List" as one species of genus Panax (family Araliaceae). Panax notoginseng saponins (PNS) are the major active ingredients extracted from Panax notoginseng.


AIM OF THE STUDY
This study investigated whether PNS and the active constituent Ginsenoside Rb1 inhibits adhesion events by regulating the NF-E2-related factor 2 (Nrf2) - p38 - vascular cell adhesion molecule (VCAM)-1 pathway.


MATERIALS AND METHODS
The AS model rats were treated once daily with PNS (100mg/kg, i.p.) or Rb1 (40mg/kg, i.p.), and pathological changes in the aortas were observed by electron microscopy and Sudan IV staining. The serum levels of NO, superoxide dismutase (SOD) and TNF-α were measured. Upon treatment with H2O2 to induce oxidative stress, cell viability and LDH levels were measured after cells were cultured with PNS or Rb1. oxidized low density lipoprotein (oxLDL)-induced VCAM-1 and p38 protein expression and THP1 cell adhesion to ECs were assessed after treatment with PNS or Rb1. Nuclear translocation of Nrf2 and expression of its target protein heme oxygenase (HO)-1 were observed in the respective presence of PNS or Rb1.


RESULTS
Upon treatment with PNS or Rb1, pathological changes observed in the aortas of AS model rats were alleviated, and an increase in serum levels of NO and SOD and a decrease in TNF-α levels were observed. In vitro treatment with PNS or Rb1 protected endothelial cells (ECs) from H2O2-mediated cytotoxicity, suppressed oxLDL-induced p38 and VCAM-1 protein expression and inhibited THP1 cell adhesion to ECs. Finally, PNS and Rb1 treatment functionally activated Nrf2 in ECs.


CONCLUSIONS
Nrf2, an EC protective system, suppresses monocyte adhesion events via the inhibition of the ROS - TNF-α - p38 - VCAM-1 pathway following treatment with PNS, with Rb1 specifically playing an important role among PNS active components.
['Fan|Jishan|J|', 'Liu|Danning|D|', 'He|Cuiyao|C|', 'Li|Xiaohui|X|', 'He|Fengtian|F|']
D000818:Animals D001011:Aorta D001018:Aortic Diseases D050197:Atherosclerosis D002448:Cell Adhesion D045744:Cell Line, Tumor D002465:Cell Movement D018920:Coculture Techniques D019610:Cytoprotection D004305:Dose-Response Relationship, Drug D004365:Drugs, Chinese Herbal D036145:Ginsenosides D061307:Human Umbilical Vein Endothelial Cells D008297:Male D009000:Monocytes D051267:NF-E2-Related Factor 2 D009569:Nitric Oxide D053723:Panax notoginseng D008517:Phytotherapy D010946:Plants, Medicinal D017208:Rats, Wistar D012503:Saponins D015398:Signal Transduction D013482:Superoxide Dismutase D014409:Tumor Necrosis Factor-alpha D019010:Vascular Cell Adhesion Molecule-1 D015054:Zymosan D048051:p38 Mitogen-Activated Protein Kinases
Panax notoginseng saponins Rb1 Nrf2 P38 VCAM-1 Endothelial cells Ginsenoside Rb1 (PubChem CID: 9898279) Hydrogen peroxide (PubChem CID: 784) Sulforaphane (PubChem CID: 5350)