Insulin receptor substrate 1 gene expression is strongly up-regulated by HSPB8 silencing in U87 glioma cells.
Objective. The aim of the present investigation was to study the expression of genes encoding IRS1 (insulin receptor substrate 1) and some other functionally active proteins in U87 glioma cells under silencing of polyfunctional chaperone HSPB8 for evaluation of the possible significance of this protein in intergenic interactions.Methods. Silencing of HSPB8 mRNA was introduced by HSPB8 specific siRNA. The expression level of HSPB8, IRS1, HK2, GLO1, HOMER3, MYL9, NAMPT, PER2, PERP, GADD45A, and DEK genes was studied in U87 glioma cells by quantitative polymerase chain reaction.Results. It was shown that silencing of HSPB8 mRNA by specific to HSPB8 siRNA led to a strong down-regulation of this mRNA and significant modification of the expression of IRS1 and many other genes in glioma cells: strong up-regulated of HOMER3, GLO1, and PERP and down-regulated of MYL9, NAMPT, PER2, GADD45A, and DEK gene expressions. At the same time, no significant changes were detected in the expression of HK2 gene in glioma cells treated by siRNA, specific to HSPB8. Moreover, the silencing of HSPB8 mRNA enhanced the glioma cells proliferation rate.Conclusions. Results of this investigation demonstrated that silencing of HSPB8 mRNA affected the expression of IRS1 gene as well as many other genes encoding tumor growth related proteins. It is possible that the dysregulation of most of the studied genes in glioma cells after silencing of HSPB8 is reflected by a complex of intergenic interactions and that this polyfunctional chaperone is an important factor for the stability of genome function and regulatory mechanisms contributing to the tumorigenesis control.
['Hnatiuk|Oksana S|OS|', 'Tsymbal|Dariia O|DO|', 'Minchenko|Dmytro O|DO|', 'Khita|Olena O|OO|', 'Viletska|Yulia M|YM|', 'Rundytska|Olha V|OV|', 'Kozynkevych|Halyna E|HE|', 'Maslak|Hanna S|HS|', 'Minchenko|Oleksandr H|OH|']
D045744:Cell Line, Tumor D015972:Gene Expression Regulation, Neoplastic D020868:Gene Silencing D005910:Glioma D006360:Heat-Shock Proteins D006801:Humans D055504:Insulin Receptor Substrate Proteins D018832:Molecular Chaperones D012333:RNA, Messenger D015854:Up-Regulation
U87 glioma cells IRS1 HOMER3 MYL9 PER2 PERP GADD45A CLO1 mRNA expression silencing HSPB8