Analysis of metabolic changes in Trichoderma asperellum TJ01 at different fermentation time-points by LC-QQQ-MS.
Trichoderma spp. are among the most widely recognized biocontrol fungi used to inhibit pathogens and promote plant growth. These functions are related to primary and secondary metabolites. This study investigated the different metabolites in Trichoderma asperellum TJ01 cultured for 24 and 72 h using liquid chromatography with triple-quadrupole mass spectrometry. Compared to the 24 h culture of T. asperellum TJ01, the 72 h culture with amino acid metabolism tended to decrease while sugar and lipid metabolisms tended to increase. Furthermore, the 72 h culture had a higher proportion of upregulated flavonoids, in combination with a higher proportion of downregulated alkaloids, and equal proportions of upregulated and downregulated polyphenols and hormones. This study also identified a few valuable medicinal substances such as trigonelline and 5-hydroxytryptophan in T. asperellum TJ01 fermentation cultures.
['Ni|Mi|M|', 'Wu|Qiong|Q|', 'Wang|Gui S|GS|', 'Liu|Qian Q|QQ|', 'Yu|Mei X|MX|', 'Tang|Jun|J|']
D006916:5-Hydroxytryptophan D000470:Alkaloids D000596:Amino Acids D002853:Chromatography, Liquid D005285:Fermentation D005419:Flavonoids D050356:Lipid Metabolism D013058:Mass Spectrometry D059808:Polyphenols D064210:Secondary Metabolism D014242:Trichoderma
fermentation metabolites triple-quadrupole mass spectrometry different time-points