Cooperation of Aspergillus nidulans enzymes increases plant polysaccharide saccharification.
Efficient polysaccharide degradation depends on interaction between enzymes acting on the main chain and the side chains. Previous studies demonstrated cooperation between several enzymes, but not all enzyme combinations have been explored. A better understanding of enzyme cooperation would enable the design of better enzyme mixtures, optimally profiting from synergistic effects. In this study, we analyzed the cooperation of several enzymes involved in the degradation of xylan, glucan, xyloglucan and crude plant biomass from Aspergillus nidulans by single and combined incubations with their polymeric substrate. Positive effects were observed between most enzymes, although not always to the same extent. Moreover, the tailor made cocktails formulated in this study resulted in efficient release of glucose from plant biomass. This study also serves as an example for the complex cooperation that occurs between enzymes in plant biomass saccharification and how expression in easily-accessible hosts, such as Pichia pastoris, can help in revealing these effects.
['Tramontina|Robson|R|', 'Robl|Diogo|D|', 'Maitan-Alfenas|Gabriela Piccolo|GP|', 'de Vries|Ronald P|RP|']
D001233:Aspergillus nidulans D018533:Biomass D005656:Fungal Proteins D005936:Glucans D006868:Hydrolysis D010843:Pichia D010944:Plants D011134:Polysaccharides D031786:Saccharum D014990:Xylans
Aspergillus nidulans Saccharification Enzymatic cocktails Polysaccharide hydrolysis Recombinant enzymes