Comparison of cardiac stem cell sheets detached by Versene solution and from thermoresponsive dishes reveals similar properties of constructs.
Cell sheets (CS) from c-kit+ cardiac stem cell (CSC) hold a potential for application in regenerative medicine. However, manufacture of CS may require thermoresponsive dishes, which increases cost and puts one in dependence on specific materials. Alternative approaches were established recently and we conducted a short study to compare approaches for detachment of CS from c-kit+ CSC. Our in-house developed method using chelation by Versene solution was compared to UpCell™ thermoresponsive plates in terms of CSC proliferation, viability, gap junction formation and engraftment in a model of myocardial infarction. Use of Versene solution instead of thermoresponsive dishes resulted in comparable CS thickness (approximately 100mcm), cell proliferation rate and no signs of apoptosis detected in both types of constructs. However, we observed a minor reduction of gap junction count in Versene-treated CS. At day 30 after delivery to infarcted myocardium both types of CS retained at the site of transplantation and contained comparable amounts of proliferating cells indicating engraftment. Thus, we may conclude that detachment of CS from c-kit+ CSC using Versene solution followed by mechanical treatment is an alternative to thermoresponsive plates allowing use of routinely available materials to generate constructs for cardiac repair.
['Dergilev|Konstantin V|KV|', 'Makarevich|Pavel I|PI|', 'Tsokolaeva|Zoya I|ZI|', 'Boldyreva|Maria A|MA|', 'Beloglazova|Irina B|IB|', 'Zubkova|Ekaterina S|ES|', 'Menshikov|Mikhail Yu|MY|', 'Parfyonova|Yelena V|YV|']
D000818:Animals D017209:Apoptosis D018929:Cell Culture Techniques D049109:Cell Proliferation D002469:Cell Separation D002470:Cell Survival D004492:Edetic Acid D017629:Gap Junctions D006801:Humans D009203:Myocardial Infarction D009206:Myocardium D051381:Rats D044968:Regenerative Medicine D033581:Stem Cell Transplantation D013234:Stem Cells
Cardiac stem cell Cell sheet Thermoresponsive dish Myocardial infarction Tissue engineering