Influence of patient related factors on number of mesenchymal stromal cells reached after in vitro culture expansion for clinical treatment.
BACKGROUND
Number of stromal cells injected in patients with ischaemic heart disease (IHD) may be of importance for the treatment efficacy, which in turn may be influenced by various patient-related factors. In this study, we investigate whether patient-related factors influence the number of autologous stromal cells reached after in vitro culture expansion for clinical therapy.


METHODS
Culture expansion data from 111 patients with IHD treated with autologous stromal cells in three clinical trials were used. We correlated the final cell count after two passages of cultivation with different patient factors.


RESULTS
There was a significant relation between body mass index (BMI) and the number of adipose derived stromal cells (ASCs) reached after culture expansion and for all patients included into the three studies (r = 0.375, p = .019 and r = 0.200, p = .036, respectively). Moreover, there was a significantly higher number of ASCs reached in patients with hypertension compared to those without hypertension and for all patients overall (68.8 ± 39.6 × 106 vs. 39.1 ± 23.6 × 106, p = .020 and 62.0 ± 55.0 × 106 vs. 29.0 ± 19.3 × 106, p < .001, respectively). The same tendency was seen with bone marrow derived mesenchymal stromal cells (MSCs) in patients with hypertension compared to those without hypertension (58.4 ± 61.8 × 106 vs. 22.6 ± 13.3 × 106, p < .001) and in males compared to females (56.4 ± 61.5 × 106 vs. 30.9 ± 27.9 × 106, p = .041). Moreover, a significant negative correlation between left ventricular ejection fraction and number of MSCs was found (r = -0.287, p = .017).


CONCLUSIONS
Patient related factors such as BMI, hypertension and gender may influence the number of MSCs reached after in vitro culture expansion.
['Qayyum|Abbas Ali|AA|', 'Kaur|Kamal Preet|KP|', 'Mathiasen|Anders Bruun|AB|', 'Haack-Sørensen|Mandana|M|', 'Ekblond|Annette|A|', 'Kastrup|Jens|J|']
D000368:Aged D015992:Body Mass Index D002452:Cell Count D018929:Cell Culture Techniques D049109:Cell Proliferation D002784:Cholesterol D005260:Female D006801:Humans D008297:Male D045164:Mesenchymal Stem Cell Transplantation D059630:Mesenchymal Stem Cells D010641:Phenotype D013318:Stroke Volume
mesenchymal stromal cells ischaemic heart disease Cell therapy coronary artery disease heart failure