Identification and molecular characterization of a peritrophin-like gene, involved in the antibacterial response in Chinese mitten crab, Eriocheir sinensis.
Peritrophin was first isolated from insect peritrophic membrane (PM) and was thought to protect insects from invasion of microorganisms and to stimulate digestion of food. In this study, a peritrophin-like gene (EsPT) was obtained from Eriocheir sinensis. The full length cDNA of EsPT was 1232 bp, which contained 1005 bp ORF encoding a protein of 334 amino acids, including a 22 amino acid signal peptide, and 3 conserved chitin binding type 2 domains (ChtBD2) characterized by having a 6-cysteine motif. Phylogenetic analysis showed that EsPT was clustered together with 2 insect peritrophin-44-like proteins (MdP44L from Musca domestica and CcP44L from Ceratitis capitata), an insect chitin binding peritrophin-A domain containing protein (CfPT from Coptotermes formosanus) and a crustacean peritrophin (MnPT from Macrobrachium nipponense). Tissue distribution analysis revealed that EsPT was mainly expressed in hepatopancreas, intestine and hemocytes. The expression of EsPT is regulated by lipopolysaccharide, peptidoglycan, Staphylococcus aureus, Vibrio parahaemolyticus and Aeromonas hydrophila challenge. The recombinant EsPT could bind to different microbes, and enhanced the clearance of V. parahaemolyticus in vivo. In crabs, silencing of EsPT by siRNA suppressed the elimination of V. parahaemolyticus and increasing number of bacteria, finally upregulated the expression of anti-lipopolysaccharide factor (ALF) and clip domain serine proteases (cSP). The results might indicate that EsPT was involved in the anti-bacterial innate immunity of crabs.
['Huang|Ying|Y|', 'Ma|FuTong|F|', 'Wang|Wen|W|', 'Ren|Qian|Q|']
D016980:Aeromonas hydrophila D000595:Amino Acid Sequence D000818:Animals D000900:Anti-Bacterial Agents D023181:Antimicrobial Cationic Peptides D060829:Arthropod Proteins D001483:Base Sequence D003386:Brachyura D002352:Carrier Proteins D006434:Hemocytes D043143:Hepatopancreas D007113:Immunity, Innate D007422:Intestines D008070:Lipopolysaccharides D008562:Membrane Glycoproteins D008969:Molecular Sequence Data D010457:Peptidoglycan D034622:RNA Interference D034741:RNA, Small Interfering D016415:Sequence Alignment D017422:Sequence Analysis, DNA D057057:Serine Proteases D013211:Staphylococcus aureus D014736:Vibrio parahaemolyticus
Peritrophin-like gene Eriocheir sinensis Innate immunity Bacteria clearance Expression pattern