A novel electrochemical assay for chymosin determination using a label-free peptide as a substrate.
Chymosin is a predominant enzyme in rennet and is used in cheese production because of its excellent milk-clotting activity. Herein, we proposed a facile and label-free electrochemical method for determining chymosin activity based on a peptide-based enzyme substrate. The synthesized substrate peptide for chymosin was assembled onto the surface of the Au-deposited grassy carbon electrode. The current was proportional to chymosin activity, and thus chymosin activity could be determined. The detection ranges of chymosin activity were 2.5 to 25 U mL-1. The detection limit of chymosin activity was 0.8 U mL-1. The sensing platform was used to quantify chymosin activity in commercial rennet with high selectivity, excellent stability, and satisfactory reproducibility. We developed a facile, fast, and effective electrochemical assay for detecting chymosin activity, which has potential applications in cheesemaking.
['Li|Can-Peng|CP|', 'Liu|Feng|F|', 'Zheng|Jing|J|', 'Zhao|Hui|H|']
D000818:Animals D002611:Cheese D012085:Chymosin D008892:Milk D010455:Peptides D015203:Reproducibility of Results
electrochemical assay chymosin cheesemaking