Effect of gastric cancer stem cell on gastric cancer invasion, migration and angiogenesis.
Purpose: Using the gastric cancer cell line SGC7901 and gastric cancer stem cell (CSC-G), we conducted this study to investigate the role of cancer stem cells in invasion, metastasis and tumor angiogenesis. Methods: Stem cell markers (OCT4, SOX2, C-Myc and Klf4) expression was detected by RT-PCR and Western blotting. The proliferation, migration, invasion abilities, L-OHP and 5-FU resistance, angiogenesis were assessed using in vitro spherical clone formation assays, plate cloning experiments, transwell migration, transwell invasion, drug resistance, scratch-wound migration, ring formation assay, and their tumorigenic and ability were assessed using a tumor formation experiment in mice. Results: Compared with the SGC7901, the expression of Oct4, Sox2, Klf4 and CD44 mRNA was significantly higher in CSC-G, the mRNA relative expression of E-cadherin in CSC-G was lower than SGC7901, while the expression of c-Myc did not significantly change. The proliferation, drug resistance, migration, and invasion abilities were significantly higher in CSC-G, and the tumorigenic ability in mice was also significantly higher. Conclusion: The proliferation, drug resistance, migration, invasion, and tumorigenic abilities of CSC-G significantly were higher than SGC7901. CSC-G plays important roles in proliferation, migration, invasion, and tumorigenicity.
['Zhu|Zhipeng|Z|', 'Xu|Jiuhua|J|', 'Li|Lulu|L|', 'Ye|Weipeng|W|', 'Xu|Guoxing|G|', 'Chen|Borong|B|', 'Zeng|Junjie|J|', 'Li|Jiayi|J|', 'Huang|Zhengjie|Z|']
D000818:Animals D015703:Antigens, CD D000970:Antineoplastic Agents D015820:Cadherins D045744:Cell Line, Tumor D002465:Cell Movement D019008:Drug Resistance, Neoplasm D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D018960:Hyaluronan Receptors D000090062:Kruppel-Like Factor 4 D016688:Mice, Inbred NOD D014411:Neoplastic Stem Cells D009389:Neovascularization, Pathologic D050814:Octamer Transcription Factor-3 D060888:Real-Time Polymerase Chain Reaction D055748:SOXB1 Transcription Factors D013274:Stomach Neoplasms D023041:Xenograft Model Antitumor Assays
Gastric cancer Cancer stem cell Tumor angiogenesis