Resident fibroblast expansion during cardiac growth and remodeling.
Cardiac fibrosis, denoted by the deposition of extracellular matrix, manifests with a variety of diseases such as hypertension, diabetes, and myocardial infarction. Underlying this pathological extracellular matrix secretion is an expansion of fibroblasts. The mouse is now a common experimental model system for the study of cardiovascular remodeling and elucidation of fibroblast responses to cardiac growth and stress is vital for understanding disease processes. Here, using diverse but fibroblast specific markers, we report murine fibroblast distribution and proliferation in early postnatal, adult, and injured hearts. We find that perinatal fibroblasts and endothelial cells proliferate at similar rates. Furthermore, regardless of the injury model, fibroblast proliferation peaks within the first week after injury, a time window similar to the period of the inflammatory phase. In addition, fibroblast densities remain high weeks after the initial insult. These results provide detailed information regarding fibroblast distribution and proliferation in experimental methods of heart injury.
['Ivey|Malina J|MJ|', 'Kuwabara|Jill T|JT|', 'Pai|Jonathan T|JT|', 'Moore|Richard E|RE|', 'Sun|Zuyue|Z|', 'Tallquist|Michelle D|MD|']
D000818:Animals D000831:Animals, Newborn D051792:Basic Helix-Loop-Helix Transcription Factors D002460:Cell Line D019070:Cell Lineage D049109:Cell Proliferation D003094:Collagen D042783:Endothelial Cells D005260:Female D005347:Fibroblasts D006321:Heart D007545:Isoproterenol D008297:Male D008810:Mice, Inbred C57BL D009203:Myocardial Infarction D011312:Pressure D011943:Receptors, Adrenergic, beta D020257:Ventricular Remodeling
Fibrosis Proliferation Endothelial cell Cardiac fibroblast