Determination of antibiotics and detergent residues in decellularized tissue-engineered heart valves using LC-MS/MS.
Residual chemicals that are presented during tissue processing in human tissue banks can be a risk for the allograft recipient. Determine the residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 months. A total of 24 human decellularized allografts were stored in sterile sodium chloride and analyzed immediately after the decellularization process (0 months) and after storage for 6, 12, and 18 months, which includes the use of sodium dodecyl sulfate (SDS) and antibiotics (cefoxitin, vancomycin hydrochloride, lincomycin hydrochloride, polymyxin B sulfate). These valves were used for suitability tests, the zone of inhibition evaluation, and direct contact cytotoxicity assay. The stock solution from 32 valves was used for LC-MS/MS analysis of antibiotics and SDS. Tissue samples from decellularized valves showed a zone of inhibition formation for S. aureus and B. subtilis, suggesting the presence of an inhibitory molecule in the tissue. Cytotoxicity tests were negative. Polymyxin B, vancomycin, and SDS were detected and quantified in human decellularized aortic and pulmonary allografts during all periods of the study. There were no traces of residual cefoxitin and lincomycin in the tissue stock solution. We found residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 months.
['Kraft|Leticia|L|', 'Ribeiro|Victoria Stadler Tasca|VST|', 'de Nazareno Wollmann|Luciana Cristina Ferretti|LCF|', 'Suss|Paula Hansen|PH|', 'Tuon|Felipe Francisco|FF|http://orcid.org/0000-0003-3471-1786']
D000818:Animals D000900:Anti-Bacterial Agents D001419:Bacteria D016923:Cell Death D002853:Chromatography, Liquid D003902:Detergents D005347:Fibroblasts D006351:Heart Valves D006801:Humans D051379:Mice D008826:Microbial Sensitivity Tests D053719:Tandem Mass Spectrometry D023822:Tissue Engineering
Decellularization Allografts Aortic valve Cardiovascular tissue banking Pulmonary valve Residue analysis