Phytotoxic and genotoxic effects of silver nanoparticles exposure on germinating wheat seedlings.
We investigated the effects of 1 and 10 mg L(-1) AgNPs on germinating Triticum aestivum L. seedlings. The exposure to 10 mg L(-1) AgNPs adversely affected the seedling growth and induced morphological modifications in root tip cells. TEM analysis suggests that the observed effects were due primarily to the release of Ag ions from AgNPs. To gain an increased understanding of the molecular response to AgNP exposure, we analyzed the genomic and proteomic changes induced by AgNPs in wheat seedlings. At the DNA level, we applied the AFLP technique and we found that both treatments did not induce any significant DNA polymorphisms. 2DE profiling of roots and shoots treated with 10 mg L(-1) of AgNPs revealed an altered expression of several proteins mainly involved in primary metabolism and cell defense.
['Vannini|Candida|C|', 'Domingo|Guido|G|', 'Onelli|Elisabetta|E|', 'De Mattia|Fabrizio|F|', 'Bruni|Ilaria|I|', 'Marsoni|Milena|M|', 'Bracale|Marcella|M|']
D054458:Amplified Fragment Length Polymorphism Analysis D004249:DNA Damage D015180:Electrophoresis, Gel, Two-Dimensional D018525:Germination D013058:Mass Spectrometry D046529:Microscopy, Electron, Transmission D053758:Nanoparticles D018517:Plant Roots D018520:Plant Shoots D040901:Proteomics D036226:Seedlings D012834:Silver D013312:Stress, Physiological D014908:Triticum
Silver nanoparticles Triticum aestivum TEM Proteomics AFLP