Efficient production of sophorolipids by Starmerella bombicola using a corncob hydrolysate medium.
Sophorolipids (SLs) are amphiphilic compounds produced from a variety of saccharides and vegetable oils by the yeast Starmerella bombicola and related strains, and they have commercial uses as detergents. In the present study, SL production was investigated using a corncob hydrolysate (CCH) medium derived from lignocellulosic feedstocks as a source of hydrophilic carbon substrates. Excess sulfuric acid concentrations during pretreatment of the corncobs increased the furfural concentrations and turned the CCH dark brown. The optimal sulfuric acid concentration was 1% (w/v), and the treated CCH, containing 45 g/l glucose, allowed the production of 33.7 g/l of SLs following 4 days of cultivation. Additional autoclaving (121°C, 20 min) inhibited SL production and cell growth by 36% and 40%, respectively. Ammonium nitrate (0.1 g-N/l) restored SL production to the autoclaved CCH. Finally, a cost-effective SL production of 49.2 g/l, with a volumetric productivity of 12.3 g/l/day, was achieved using CCH medium during batch cultivation in a jar fermentor.
['Konishi|Masaaki|M|', 'Yoshida|Yuka|Y|', 'Horiuchi|Jun-ichi|J|']
D001203:Ascomycota D061249:Batch Cell Culture Techniques D019149:Bioreactors D003470:Culture Media D003902:Detergents D005662:Furaldehyde D006017:Glycolipids D006868:Hydrolysis D057927:Hydrophobic and Hydrophilic Interactions D008031:Lignin D010938:Plant Oils D013464:Sulfuric Acids D003313:Zea mays
Sophorolipids Starmerella bombicola Corncob hydrolysate Biosurfactant Lignocellulosic biomass Surface-active glycolipid