Kiwifruit fibre level influences the predicted production and absorption of SCFA in the hindgut of growing pigs using a combined in vivo-in vitro digestion methodology.
Combined in vivo (ileal cannulated pig) and in vitro (faecal inoculum-based fermentation) digestion methodologies were used to predict the production and absorption of SCFA in the hindgut of growing pigs. Ileal and faecal samples were collected from animals (n 7) fed diets containing either 25 or 50 g/kg DM of kiwifruit fibre from added kiwifruit for 14 d. Ileal and faecal SCFA concentrations normalised for food DM intake (DMI) and nutrient digestibility were determined. Ileal digesta were collected and fermented for 38 h using a fresh pig faecal inoculum to predict SCFA production. The predicted hindgut SCFA production along with the determined ileal and faecal SCFA were then used to predict SCFA absorption in the hindgut and total tract organic matter digestibility. The determined ileal and faecal SCFA concentrations (e.g. 8·5 and 4·4 mmol/kg DMI, respectively, for acetic acid for the low-fibre diet) represented only 0·2-3·2 % of the predicted hindgut SCFA production (e.g. 270 mmol/kg DMI for acetic acid). Predicted production and absorption of acetic, butyric and propionic acids were the highest for the high-fibre diet (P0·05). In conclusion, determined ileal and faecal SCFA concentrations represent only a small fraction of total SCFA production, and may therefore be misleading in relation to the effect of diets on SCFA production and absorption. Considerable quantities of SCFA are produced and absorbed in the hindgut of the pig by the fermentation of kiwifruit.
['Montoya|Carlos A|CA|', 'Rutherfurd|Shane M|SM|', 'Moughan|Paul J|PJ|']
D029042:Actinidia D000818:Animals D001419:Bacteria D004032:Diet D004043:Dietary Fiber D004063:Digestion D005232:Fatty Acids, Volatile D005243:Feces D005285:Fermentation D005638:Fruit D041981:Gastrointestinal Tract D007082:Ileum D007408:Intestinal Absorption D008297:Male D034421:Sus scrofa
Absorption SCFA Growing pigs DMI DM intake GIT gastrointestinal tract In vitro fermentation In vivo digestion Kiwifruit dietary fibre OM organic matter