Determination of kaurenoic acid in rat plasma using UPLC-MS/MS and its application to a pharmacokinetic study.
Kaurenoic acid (KA), a kaurane diterpene found in several medicinal plants, is an active ingredient with potential anti-inflammatory, anticonvulsant, antibacterial and antitumor activities. In this work, an ultra-performance liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS) was firstly developed and validated to quantify kaurenoic acid in rat plasma. Rhein was chosen as the internal standard (IS) and the plasma was processed with one-step acetonitrile protein precipitation; the chromatographic separation was achieved on a HSS T3 (2.1 × 50 mm, 1.8 μm) column with the mobile phase consisting of acetonitrile and water containing 0.1% formic acid via gradient elution. An electrospray ionization source was applied and operated in the negative ion and multiple reaction monitoring (MRM) modes. Kaurenoic acid and IS were quantified using the transitions of m/z 301.2→301.2 (pseudo MRM) and m/z 283.2 → 238.9, respectively. The calibration curves were linear over the range of 5∼ 100 ng/mL (R2 = 0.990). The lower limit of quantification (LLOQ) was 5 ng/mL. The intra- and inter- day precision (RSD) ranged from 3.0% to 11.4%. The matrix effect and extraction recovery were within acceptable limits. The validated method was successfully applied to the pharmacokinetic study of kaurenoic acid in rats after oral administration at three dosages.
['Jiang|Xiunan|X|', 'Shen|Yiting|Y|', 'Wang|Haixing|H|', 'Wang|Caihong|C|', 'Ye|Xiaoxia|X|', 'Xiang|Zheng|Z|']
D000097:Acetonitriles D000284:Administration, Oral D000818:Animals D005591:Chemical Fractionation D002851:Chromatography, High Pressure Liquid D004224:Diterpenes D008297:Male D010936:Plant Extracts D051381:Rats D017207:Rats, Sprague-Dawley D015203:Reproducibility of Results D021241:Spectrometry, Mass, Electrospray Ionization D053719:Tandem Mass Spectrometry
Kaurenoic acid UPLC-MS/MS Pharmacokinetic Bioavailability