Inhibition of Frizzled-2 by small interfering RNA protects rat hepatic BRL-3A cells against cytotoxicity and apoptosis induced by Hypoxia/Reoxygenation.
Frizzled-2 plays an important role in maintaining normal hepatic cell functionality. This study aimed to investigate the role of inhibition of Frizzled-2 in protecting rat liver BRL-3A cells from Hypoxia/Reoxygenation (H/R). In vitro H/R hepatic cell model was established by culturing BRL-3A cells under H/R condition. Frizzled-2 siRNA was transfected into BRL-3A cells to inhibit Frizzled-2 signaling. Wnt5a and Frizzled-2 were significantly increased in BRL-3A cells upon H/R treatment. H/R treatment induced cell cytotoxicity, the early apoptosis rate and the intracellular Ca2+ level in BRL-3A cells while silencing frizzled-2 gene decreased the H/R induced cell cytotoxicity, apoptosis and intracellular Ca2+ level. In vivo mice study further showed the up-regulation of Frizzled-2/Wnt 5 pathway and cleaved Caspase-3 expression in liver tissues under ischemia and reperfusion injury (IRI). In summary, inhibition of Frizzled-2 by its siRNA may protects BRL-3A cells by attenuating the H/R induced cell cytotoxicity and apoptosis.
['Hu|Xiang|X|', 'Zhou|Chenjie|C|', 'He|Guolin|G|', 'Cheng|Yuan|Y|', 'Pan|Mingxin|M|', 'Gao|Yi|Y|']
D000818:Animals D017209:Apoptosis D020013:Calcium Signaling D053148:Caspase 3 D015687:Cell Hypoxia D002460:Cell Line D051157:Frizzled Receptors D005786:Gene Expression Regulation D022781:Hepatocytes D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D034622:RNA Interference D034741:RNA, Small Interfering D051381:Rats D015427:Reperfusion Injury D060449:Wnt Signaling Pathway D000071818:Wnt-5a Protein D051176:beta Catenin
Frizzled-2 Hepatic BRL-3A cells Apoptosis Hypoxia/Reoxygenation (H/R) siRNA In vivo mice study ARNip Ca(2+) intracelular Estudio de ratones in vivo Hepatocitos BRL-3A Hipoxia/reoxigenación Intracellular Ca(2+)