Detection of misidentifications of species from the Burkholderia cepacia complex and description of a new member, the soil bacterium Burkholderia catarinensis sp. nov.
The correct identification of bacteria from the Burkholderia cepacia complex (Bcc) is crucial for epidemiological studies and treatment of cystic fibrosis infections. However, genome-based identification tools are revealing many controversial Bcc species assignments. The aim of this work is to re-examine the taxonomic position of the soil bacterium B. cepacia 89 through polyphasic and genomic approaches. recA and 16S rRNA gene sequence analysis positioned strain 89 inside the Bcc group. However, based on the divergence score of seven concatenated allele sequences, and values of average nucleotide identity, and digital DNA:DNA hybridization, our results suggest that strain 89 is different from other Bcc species formerly described. Thus, we propose to classify Burkholderia sp. 89 as the novel species Burkholderia catarinensis sp. nov. with strain 89T (=DSM 103188T = BR 10601T) as the type strain. Moreover, our results call the attention to some probable misidentifications of Bcc genomes at the National Center for Biotechnology Information database.
['Bach|Evelise|E|', "Sant'Anna|Fernando Hayashi|FH|", 'Magrich Dos Passos|João Frederico|JF|', 'Balsanelli|Eduardo|E|', 'de Baura|Valter Antonio|VA|', 'Pedrosa|Fábio de Oliveira|FO|', 'de Souza|Emanuel Maltempi|EM|', 'Passaglia|Luciane Maria Pereira|LMP|']
D019117:Burkholderia D042602:Burkholderia cepacia complex D058893:DNA Barcoding, Taxonomic D004269:DNA, Bacterial D018703:Founder Effect D016680:Genome, Bacterial D053858:Metabolic Networks and Pathways D009693:Nucleic Acid Hybridization D010802:Phylogeny D012336:RNA, Ribosomal, 16S D011938:Rec A Recombinases D017422:Sequence Analysis, DNA D012988:Soil Microbiology D013045:Species Specificity D009626:Terminology as Topic
misidentification Burkholderia cepacia complex Burkholderia catarinensis genome ANI MLSA