Comparison of the results of preimplantation genetic screening obtained by a-CGH and NGS methods from the same embryos.
Chromosomal aneuploidies are known for being the main cause of abnormal development of embryos with normal morphology, their implantation failure and early reproductive losses in IVF treatments. Preimplantation genetic screening (PGS) allows selecting embryos with normal chromosomal content and increases IVF treatment efficiency due to higher implantation rates and less frequent early pregnancy losses. New technologies used for PGS allow making genome-wide analysis of the presence of all chromosomes in embryos. This article presents our study of evaluation of two techniques used for PGS: previously developed and used in our laboratory a-CGH assay based on Agilent technology and newly tested semi-conductive NGS technique (Torrent technology).
['Aleksandrova|N|N|', 'Shubina|E|E|', 'Ekimov|A|A|', 'Kodyleva|T|T|', 'Mukosey|I|I|', 'Makarova|N|N|', 'Kulakova|E|E|', 'Levkov|L|L|', 'Trofimov|D|D|', 'Sukhikh|G|G|']
D055028:Comparative Genomic Hybridization D004624:Embryo Transfer D005260:Female D005820:Genetic Testing D006801:Humans D019836:Preimplantation Diagnosis D017422:Sequence Analysis, DNA
preimplantation genetic screening IVF Array comparative genomic hybridization assisted reproductive technologies embryo aneuploidy next-generation sequencing