Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy.
BACKGROUND
Levels of specific IgE (sIgE) against allergen components can be assessed using multiplex assays or with highly sensitive, quantitative methods. The aim of this study was to compare the sensitivity and specificity of different immunological methods for diagnosis of shrimp allergy.


METHODS
Twenty patients with positive skin prick tests for frozen tiger shrimp were selected for further examination. Blood samples were taken to assess concentrations of sIgE against the house dust mites Dermatophagoides pteronyssinus and D. farinae, shrimp allergen extract, allergen components Der p 1, Der p 2 and Pan a 1 (ImmunoCap), and the ImmunoCap ISAC 112 panel.


RESULTS
All patients had elevated levels of sIgE against shrimp and D pteronyssinus. Eight patients were sensitized to Pen m 1, three patients were sensitized to Pen m 2, and two patients were sensitized to Pen m 4 (ISAC). ImmunoCap ISAC detected shrimp sensitization in 50% of patients. There was a strong correlation between concentrations of sIgE against Pen m1 and Der p 10 detected by ImmunoCap.


CONCLUSIONS
The singleplex ImmunoCap system remains the reference diagnostic method, but in the case of shrimp allergy ImmunoCap ISAC provided better insight into patient allergen profiles.
['Ukleja-Sokołowska|Natalia|N|https://orcid.org/0000-0001-5957-8382', 'Lis|Kinga|K|', 'Żbikowska-Gotz|Magdalena|M|', 'Adamczak|Rafał|R|', 'Kuźmiński|Andrzej|A|', 'Bartuzi|Zbigniew|Z|']
D000485:Allergens D000818:Animals D006801:Humans D006967:Hypersensitivity D007073:Immunoglobulin E D039981:Pyroglyphidae D012882:Skin Tests
shrimp Allergy IgE sensitization in vitro diagnostics tropomyosin