MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.
Long noncoding RNAs (lncRNAs) play a crucial role in progression of bladder urothelial carcinoma (BUC). However, the molecular mechanisms behind this role have not been elucidated yet. Here, we found that the lncRNA MAFG-AS1, which is highly expressed in BUC, is correlated with aggressive characteristics and poor prognosis of BUC. We demonstrate that MAFG-AS1 can promote BUC proliferation, invasion, metastasis, and epithelial-mesenchymal transition in vitro and in vivo. Mechanistically, MAFG-AS1 direct binding to Hu antigen R (HuR) could recruit ubiquitin-specific proteinase 5 (USP5) to prevent HuR from degrading by ubiquitination. We further demonstrate that overexpression of MAFG-AS1 can upregulate the expression of polypyrimidine tract-binding protein 1 (PTBP1) through promoting its stability mediated by bound HuR. In conclusion, these findings indicate that MAFG-AS1 promotes the progression of BUC via regulation of the HUR/PTBP1 axis. Targeting MAFG-AS1 may provide a novel strategy for individualized therapy and a potential biomarker for prognosis of BUC.
['Xiao|Mengqing|M|', 'Liu|Jianye|J|', 'Xiang|Liang|L|', 'Zhao|Kai|K|', 'He|Dong|D|', 'Zeng|Qinghai|Q|', 'Zhang|Qun|Q|', 'Xie|Dan|D|', 'Deng|Minhua|M|', 'Zhu|Yuxing|Y|', 'Zhang|Yeyu|Y|', 'Liu|Yan|Y|', 'Bo|Hao|H|', 'Liu|Xiaoming|X|', 'Chen|Xingyu|X|', 'Gong|Lian|L|', 'Bao|Ying|Y|', 'Hu|Yi|Y|', 'Cheng|Yaxin|Y|', 'Deng|Liping|L|', 'Zhu|Rongrong|R|', 'Xing|Xiaowei|X|', 'Zhou|Ming|M|', 'Xiong|Wei|W|', 'Zhou|Yanhong|Y|', 'Zhou|Jianda|J|', 'Li|Xiaohui|X|', 'Cao|Ke|K|https://orcid.org/0000-0001-5392-2306']
MAFG-AS1 HuR PTBP1 bladder urothelial carcinoma