Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors.
Manufacturing chimeric antigen receptor (CAR)-modified T cells requires incorporation of the CAR transgene, for which viral vectors are most often used. Here, we describe the generation of CAR T cells using primary human T cells and a non-self-inactivating gammaretroviral vector encoding a CAR transgene. The gammaretroviral vector is produced by 293T cells transiently transfected with DNA plasmids encoding necessary components of the viral vector. The resulting viral particles efficiently infect activated T cells and integrate the CAR transgene into the genome of dividing cells for stable expression.
['Mo|Feiyan|F|', 'Mamonkin|Maksim|M|']
D000818:Animals D002460:Cell Line D017988:Gammaretrovirus D015870:Gene Expression D005818:Genetic Engineering D005822:Genetic Vectors D006801:Humans D016130:Immunophenotyping D016219:Immunotherapy, Adoptive D008213:Lymphocyte Activation D011948:Receptors, Antigen, T-Cell D000076962:Receptors, Chimeric Antigen D013601:T-Lymphocytes D014161:Transduction, Genetic D019076:Transgenes
Chimeric antigen receptors Gammaretroviral vectors CAR T cell generation T cell transduction Vector production