Serum microRNA let-7a-1/let-7d/let-7f and miRNA 143/145 Gene Expression Profiles as Potential Biomarkers in HCV Induced Hepatocellular Carcinoma.
BACKGROUND
Egypt has the highest prevalence of hepatitis C virus (HCV) worldwide. Which make liver cirrhosis and hepatocellular carcinoma (HCC) major health concerns in Egypt. Circulating microRNAs (miRNAs) have been investigated as biomarkers for malignancies. We investigated miRNA gene expression of Lethal-7 (let-7) cluster: let7-a-1, let-7d-1, let-7f-1 and miRNA (miR)143/145 cluster in sera of HCC patients and chronic HCV patients.


METHODS
The study included 40 post HCV-Hepatocellular carcinoma patients, 40 chronic HCV patients divided into 2 subgroups, 20 cirrhotic patients and 20 non-cirrhotic patients, and 40 apparently healthy subjects as a control group. Gene expression analysis for studied miRNAs was done using quantitative SYBR Green reverse-transcription Real-Time polymerase chain reaction (PCR).


RESULTS
We found that Let-7a-1 gene expression was significantly downregulated in the serum of HCV-HCC patients than in HCV non HCC cirrhotic group and was significantly upregulated in the serum of liver cirrhosis patients than HCV non-cirrhotic group. miR-143 and miR-145 expressions were significantly downregulated in the serum of HCC patients than in control group and miR-143 was significantly downregulated in the serum of non-cirrhotic HCV patients than in control group.


CONCLUSION
The downregulation of serum let-7-a1, miR-143, and miR-145 gene expression may exhibit significant influence on the development of HCC in chronic HCV Egyptian patients and can be used as biomarkers for HCC diagnosis.<br />.
['Aly|Doaa Mamdouh|DM|', 'Gohar|Nadida Abdul-Hameed|NA|', 'Abd El-Hady|Afaf Ahmed|AA|', 'Khairy|Marwa|M|', 'Abdullatif|Mona Mohsen|MM|']
D000368:Aged D006528:Carcinoma, Hepatocellular D016022:Case-Control Studies D005260:Female D019698:Hepatitis C, Chronic D006801:Humans D008103:Liver Cirrhosis D008113:Liver Neoplasms D008297:Male D035683:MicroRNAs D008875:Middle Aged D020133:Reverse Transcriptase Polymerase Chain Reaction D059467:Transcriptome D047368:Tumor Burden D000509:alpha-Fetoproteins
MicroRNA Hepatocellular carcinoma Hepatitis C Virus PCR