Anti-inflammatory naphthoates and anthraquinones from the roots of Morinda officinalis.
Morinda (Morinda officinalis) is widely consumed as a health-care herb in Asia and reported to possess various biological activities. In this study, anti-inflammatory phytochemicals were investigated and two pairs of new methyl-2-naphthoate enantiomers (1a/1b, 2a/2b), one new anthraquinone (3), three new natural unknown anthraquinones (5-6, 23), and eighteen known anthraquinones were isolated and elucidated from the roots of morinda. Anti-inflammatory activities of the isolated compounds were assessed in lipopolysaccharide-stimulated RAW 264.7 macrophages. Compounds 2b and 19 significantly inhibited the production of NO with IC50 values of 34.32 ± 4.87 and 17.17 ± 4.13 μM (indomethacin, IC50 26.71 ± 6.32 μM), and they were further corroborated via immunoblotting, quantitative real-time PCR and immunofluorescence staining assays. They could dose-dependent suppress lipopolysaccharide-stimulated pro-inflammatory factors (COX-2 and iNOS) production and block nuclear translocation of NF-κB. The results implied that reasonable consumption of morinda may be beneficial for preventing and reducing the occurrence of inflammatory-associated diseases.
['Luo|Huan|H|', 'Wang|Yi|Y|', 'Qin|Qiuyi|Q|', 'Wang|Yihai|Y|', 'Xu|Jingwen|J|', 'He|Xiangjiu|X|']
D000818:Animals D000880:Anthraquinones D000894:Anti-Inflammatory Agents, Non-Steroidal D004305:Dose-Response Relationship, Drug D008070:Lipopolysaccharides D051379:Mice D015394:Molecular Structure D032066:Morinda D009281:Naphthalenes D009569:Nitric Oxide D018517:Plant Roots D000067996:RAW 264.7 Cells D013329:Structure-Activity Relationship
Anti-inflammatory Naphthoates Anthraquinones NO Morinda officinalis How. NF-κB