Overexpression of Brassica campestris BcICE1 gene increases abiotic stress tolerance in tobacco.
In this study, a cDNA of ICE1 (inducer of CBF expression 1) gene, named BcICE1, was isolated from Brassica campestris 'Longyou 6'. The deduced protein has 499 amino acids containing a typical bHLH domain and is highly identical with AtICE1 (85.9%) from Arabidopsis thaliana. BcICE1 is located in the nucleus. The activities of SOD, CAT, POD, and APX and the transcriptional levels of SOD, CAT, and POD genes were higher in BcICE1-transgenic tobacco than in wild-type (WT) tobacco under cold stress. Compared with WT tobacco, proline, soluble sugar, and chlorophyll were enhanced, whereas malondialdehyde and relative conductivity were decreased in BcICE1-transgenic tobacco. The overexpression of BcICE1 in tobacco increased the expression of CBF1, CBF2, and other stress-related genes. Moreover, under salt and PEG (25%) stress, the activities of APX and GPX and content of soluble sugar and chlorophyll in BcICE1-transgenic tobacco were higher than those in WT tobacco. Our results suggest that BcICE1 plays an important role in the response to abiotic stress.
['Zhang|Tengguo|T|', 'Mo|Jiangnan|J|', 'Zhou|Ke|K|', 'Chang|Yan|Y|', 'Liu|Zigang|Z|']
D000222:Adaptation, Physiological D000595:Amino Acid Sequence D000975:Antioxidants D001937:Brassica D002467:Cell Nucleus D002734:Chlorophyll D003001:Cloning, Molecular D018506:Gene Expression Regulation, Plant D017343:Genes, Plant D006861:Hydrogen Peroxide D008315:Malondialdehyde D010940:Plant Proteins D030821:Plants, Genetically Modified D011392:Proline D013312:Stress, Physiological D013347:Subcellular Fractions D014026:Tobacco
Brassica campestris Abiotic stress ICE1 Transgenic plant