Galanin up-regulates the expression of M1 muscarinic acetylcholine receptor via the ERK signaling pathway in primary cultured prefrontal cortical neurons.
The expression of galanin and galanin receptors are up-regulated in the brains from patients with Alzheimer's disease (AD). However, the role of galanin in the progress of AD is still controversial. Here we demonstrated that galanin increased the protein expression of M1 muscarinic acetylcholine receptor (M1) in the primary cultured prefrontal cortical neurons by ELISA and Western Blot. Moreover, we showed that the mRNA expression of M1 was also up-regulated by galanin treatment. We further explored the mechanism of the galanin induced up-regulation of M1. We found that galanin activated the ERK signaling pathway in the primary cultured prefrontal cortical neurons. In addition, our results showed that the up-regulation of M1 mRNA was blocked by an ERK inhibitor, U0126. Taken together, our results demonstrated that the ERK signaling pathway mediated the galanin induced up-regulation of M1 in the primary cultured prefrontal cortical neurons, supporting the hypothesis that galanin plays a beneficial role in the development of AD.
['Cheng|Yong|Y|', 'Yu|Long-Chuan|LC|']
D000818:Animals D000831:Animals, Newborn D002070:Butadienes D002478:Cells, Cultured D019004:Galanin D020935:MAP Kinase Signaling System D019950:Mitogen-Activated Protein Kinase 1 D048052:Mitogen-Activated Protein Kinase 3 D009474:Neurons D009570:Nitriles D017397:Prefrontal Cortex D061251:Primary Cell Culture D051381:Rats D043643:Receptor, Muscarinic M1
Galanin M1 muscarinic acetylcholine receptor ERK Alzheimer’s disease