Tinea corporis by Microsporum canis in mycological laboratory staff: Unexpected results of epidemiological investigation.
Microsporum canis is a zoophilic dermatophyte, which is very contagious, especially to cats and dogs. Asymptomatic animal carriers of M. canis are regarded a critical factor in the epidemiology of the disease. The aim of this study was to investigate the epidemiological origin of M. canis isolates using morphological traits in combination with molecular analysis. Identification of dermatophyte strains was carried out by correlating the clinical manifestation of the infection with a micro- and macroscopic examination. To confirm the species affiliation fully, molecular differentiation methods were used. A positive result of the culture examination was obtained from the samples with arthrospores in the direct analysis, that is, from a symptomatic cat and humans, and from a cat without any signs of infection. The microsatellite-primed PCR fingerprinting (MSP-PCR) electro-profiles were identical for all analysed strains. The melting profile-PCR (MP-PCR) electrophoregram indicated variability of the genomes of the strains. The search for the source of the infection indicated one cat that did not have any signs of dermatophytosis. PCR-fingerprinting techniques are useful tools for epidemiological investigation of the origin of dermatophyte infection. These methods can also be used in many cases for species identification of dermatophytes and clarification of the relationships among varieties of a species.
['Gnat|Sebastian|S|http://orcid.org/0000-0001-9872-0046', 'Łagowski|Dominik|D|http://orcid.org/0000-0002-7846-5444', 'Nowakiewicz|Aneta|A|', 'Zięba|Przemysław|P|']
D000328:Adult D000818:Animals D002371:Cat Diseases D002415:Cats D016172:DNA Fingerprinting D005260:Female D014644:Genetic Variation D006801:Humans D007753:Laboratories D008828:Microbiological Techniques D018895:Microsatellite Repeats D008853:Microscopy D008864:Microsporum D008875:Middle Aged D017720:Molecular Epidemiology D058889:Molecular Typing D016533:Mycological Typing Techniques D014005:Tinea D015047:Zoonoses
Microsporum canis epidemiology MP-PCR dermatophytosis microsatellite primed-PCR