Anion Receptors for the Discrimination of ATP and ADP in Biological Media.
The design and synthesis of molecular receptors for the selective binding of nucleoside phosphate anions (e. g. ATP, ADP, GTP, GDP, UDP) in aqueous media at physiological pH is a valuable research endeavour, which could lead to new sensing tools for biomedical and drug discovery research. However, this target is very challenging due to similarities in anion size, structure and charge. This Minireview provides an account of the development of receptors capable of discriminating between ATP and ADP, and their utilisation in biological sensing applications. Particular focus is given to the application of receptors for the determination of ATP or ADP concentrations in biological media, tracking ATP levels (or the ATP/ADP ratio) in cells using fluorescence microscopy, or real-time monitoring of enzyme reactions involving ATP and ADP in vitro.
['Butler|Stephen J|SJ|0000-0001-8109-3330', 'Jolliffe|Katrina A|KA|']
D000244:Adenosine Diphosphate D000255:Adenosine Triphosphate D000818:Animals D015374:Biosensing Techniques D006801:Humans D015335:Molecular Probes
anion receptors fluorescence adenosine phosphates cellular imaging molecular recognition