Repression of deoxynivalenol-triggered cytotoxicity and apoptosis by mannan/β-glucans from yeast cell wall: Involvement of autophagy and PI3K-AKT-mTOR signaling pathway.
Deoxynivalenol (DON) is the most common trichothecene distributed in food and feed. So far, much work has focused on investigating the cytotoxicity of DON, while there is few researches aimed at intervening in the toxic impacts on humans and livestock posed by DON. The objective of this study is to investigate the underlying mechanism of biomacromolecules mannan/β-glucans from yeast cell wall (BYCW) for their potency to impede the cytotoxicity and apoptosis caused by DON with porcine jejunum epithelial cell lines (IPEC-J2) used as a cell injury model. We analyzed the cell morphology, cell activity, oxidative stress, fluorescence intensity and expressions of proteins relevant to autophagy, apoptosis and PI3K-AKT-mTOR signaling pathway by using inverted microscopy, MTS, reactive oxygen species (ROS), glutathione (GSH) and malondialdehyde (MDA) assay, Annexin V-FITC / propidium iodide (PI) double staining and Western blot assay. The consequent data demonstrated that in the presence of BYCW, the cell morphology and activity were relatively ameliorated and that the oxidation damage was attenuated with DON-induced autophagy concomitantly decreased, which, furthermore, was found involved in the positive regulation on PI3K-AKT-mTOR signaling pathway by BYCW. In a word, BYCW possess an ability to repress the cytotoxicity and apoptosis induced by DON through the inhibition of autophagy via activating PI3K-AKT-mTOR signaling pathway.
['Zhao|Yujie|Y|', 'Guo|Wenyan|W|', 'Gu|Xiaolian|X|', 'Chang|Chao|C|', 'Wu|Jine|J|']
D000818:Animals D017209:Apoptosis D001343:Autophagy D002460:Cell Line D049109:Cell Proliferation D002473:Cell Wall D005670:Fusarium D007583:Jejunum D008351:Mannans D018384:Oxidative Stress D019869:Phosphatidylinositol 3-Kinases D051057:Proto-Oncogene Proteins c-akt D017382:Reactive Oxygen Species D012441:Saccharomyces cerevisiae D015398:Signal Transduction D013552:Swine D058570:TOR Serine-Threonine Kinases D014255:Trichothecenes D047071:beta-Glucans
Apoptosis Mannan β-glucans Autophagy PI3K-AKT-mTOR signaling pathway Deoxynivalenol