MicroRNA-124-3p represses cell growth and cell motility by targeting EphA2 in glioma.
MiR-124-3p and EphA2 are aberrantly expressed in glioma tissue specimens. In the present study, we firstly investigated that miR-124-3p inhibits EphA2 expression mediated by binding its 3'-UTR to regulate the progression of human glioma. The U87MG and LN229 cells were transfected with miR-124-3p mimics and/or siRNA-EphA2, and then the role of miR-124-3p and EphA2 in the colony-formation, cell-cycle, migration and invasion of glioma cells in vitro were examined. Proteins involved in the epithelial-mesenchymal transition were examined using western blot. The results showed that miR-124-3p was significantly downregulated in glioma tissues, whereas a marked upregulation of EphA2 expression was found. Colony-formation and flow cytometry assays demonstrated that EphA2 downregulation or miR-124-3p mimics caused growth and cell-cycle inhibition in glioma. Transwell migration and invasion assays demonstrated that EphA2 downregulation or miR-124-3p mimics suppressed the migration and invasion of glioma cells. EphA2 downregulation or miR-124-3p mimics reduced the level of vimentin in U87MG and LN229 cells. In conclusion, miR-124-3p was found to suppress the growth, migration and invasion of glioma cells in vitro via EphA2. Furthermore, we validated miR-124-3p enforced its biological modulation via targeting EphA2 through the rescue experiment. Conclusively, our study proclaimed that miR-124-3p can counteract the malignant phenotypes of glioma cells by the inhibitory effect of the EphA2.
['Wu|Qiaoli|Q|', 'Xu|Lixia|L|', 'Wang|Chen|C|', 'Fan|Weijia|W|', 'Yan|Hua|H|', 'Li|Qingguo|Q|']
D000818:Animals D001932:Brain Neoplasms D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D058750:Epithelial-Mesenchymal Transition D015972:Gene Expression Regulation, Neoplastic D005910:Glioma D006801:Humans D008297:Male D008807:Mice, Inbred BALB C D035683:MicroRNAs D009361:Neoplasm Invasiveness D036104:Receptor, EphA2
MicroRNA-124-3p Motility EphA2 Glioma