Enumeration of Circulating Tumor Cells and Disseminated Tumor Cells in Blood and Bone Marrow by Immunomagnetic Enrichment and Flow Cytometry (IE/FC).
Enumerating circulating tumor cells (CTCs) in blood and disseminated tumor cells (DTCs) in bone marrow has shown to be clinically useful, as elevated numbers of these cells predict poor clinical outcomes. Accurate detection and quantification is, however, difficult and technically challenging because CTCs and DTCs are extremely rare. We have developed a novel quantitative detection method for enumeration of CTCs and DTCs. Our approach consists of two steps: (1) EPCAM-based immunomagnetic enrichment followed by (2) flow cytometry (IE/FC). The assay takes approximately 2 h to complete. In addition to tumor cell enumeration, IE/FC offers opportunities for direct isolation of highly pure tumor cells for downstream molecular characterization.
['Magbanua|Mark Jesus M|MJM|', 'Solanki|Tulasi I|TI|', 'Ordonez|Andrea D|AD|', 'Hsiao|Feng|F|', 'Park|John W|JW|']
D000911:Antibodies, Monoclonal D014408:Biomarkers, Tumor D001854:Bone Marrow Cells D002452:Cell Count D045744:Cell Line, Tumor D000071858:Epithelial Cell Adhesion Molecule D004867:Equipment Design D005434:Flow Cytometry D005456:Fluorescent Dyes D006801:Humans D018796:Immunoconjugates D018189:Immunomagnetic Separation D017493:Leukocyte Common Antigens D058185:Magnetite Nanoparticles D009369:Neoplasms D009360:Neoplastic Cells, Circulating D011485:Protein Binding
Enumeration Circulating tumor cells Disseminated tumor cells Immunomagnetic enrichment Flow cytometry