Enzootic bovine leukosis in a two-month-old calf.
A two-month-old calf was diagnosed with leukosis on the basis of the clinical sign of enlarged, superficial lymph nodes. Serological and genetic tests for bovine leukemia virus (BLV) were performed because the calf was born from a cow infected with BLV. The serum had a weakly positive BLV antibody, and the BLV provirus was detected within neoplastic cells on performing polymerase chain reaction (PCR). Analysis of the BLV provirus integration site using inverse PCR revealed that the BLV integration site location was identical on all chromosomes in all tumor tissues examined. Thus, the tumor cells monoclonally proliferated following BLV infection. The present study shows that enzootic bovine leukosis can occur in a young animal, as in the two-month-old calf in our study.
['Oguma|Keisuke|K|', 'Suzuki|Miho|M|', 'Sentsui|Hiroshi|H|']
D000818:Animals D000914:Antibodies, Viral D002417:Cattle D049109:Cell Proliferation D002999:Clone Cells D004279:DNA, Viral D016583:Enzootic Bovine Leukosis D018445:Infectious Disease Transmission, Vertical D001909:Leukemia Virus, Bovine D007962:Leukocytes D008198:Lymph Nodes D008297:Male D011533:Proviruses D016662:Virus Integration
Calf BLV Integration site Inverse PCR EBL