The poplar basic helix-loop-helix transcription factor BEE3 - Like gene affects biomass production by enhancing proliferation of xylem cells in poplar.
Brassinosteroids (BRs) play important roles in many aspects of plant growth and development, including regulation of vascular cambium activities and cell elongation. BR-induced BEE3 (brassinosteroid enhanced expression 3) is required for a proper BR response. Here, we identified a poplar (Populus alba × Populus glandulosa) BEE3-like gene, PagBEE3L, encoding a putative basic helix-loop-helix (bHLH)-type transcription factor. Expression of PagBEE3L was induced by brassinolide (BL). Transcripts of PagBEE3L were mainly detected in stems, with the internode having a low level of transcription and the node having a relatively higher level. The function of the PagBEE3L gene was investigated through phenotypic analyses with PagBEE3L-overexpressing (ox) transgenic lines. This work particularly focused on a potential role of PagBEE3L in stem growth and development of polar. The PagBEE3L-ox poplar showed thicker and longer stems than wild-type plants. The xylem cells from the stems of PagBEE3L-ox plants revealed remarkably enhanced proliferation, resulting in an earlier thickening growth than wild-type plants. Therefore, this work suggests that xylem development of poplar is accelerated in PagBEE3L-ox plants and PagBEE3L plays a role in stem growth by increasing the proliferation of xylem cells to promote the initial thickening growth of poplar stems.
['Noh|Seol Ah|SA|', 'Choi|Young-Im|YI|', 'Cho|Jin-Seong|JS|', 'Lee|Hyoshin|H|']
D000595:Amino Acid Sequence D051792:Basic Helix-Loop-Helix Transcription Factors D018533:Biomass D049109:Cell Proliferation D018507:Gene Expression Regulation, Developmental D018506:Gene Expression Regulation, Plant D006824:Hybridization, Genetic D018613:Microscopy, Confocal D008969:Molecular Sequence Data D010802:Phylogeny D010940:Plant Proteins D018547:Plant Stems D030821:Plants, Genetically Modified D032107:Populus D020133:Reverse Transcriptase Polymerase Chain Reaction D017386:Sequence Homology, Amino Acid D052584:Xylem
Poplar Biomass Xylem PagBEE3L Brassinosteriod bHLH