Lactobacillus terrae sp. nov., a novel species isolated from soil samples in the Republic of Korea.
A novel strain, designated NIBRBAC000499792T, was isolated from a soil sample collected at Jukgye, Dongnam, Cheonan, Republic of Korea. Cells were Gram-positive, non-motile, non-spore-forming, rod-shaped, oxidase-negative and catalase-negative. Colonies grown on de Man, Rogosa and Sharpe agar were white, circular, raised and entire. Analysis of the 16S rRNA gene sequence analysis revealed that strain NIBRBAC000499792T belongs to the genus Lactobacillus (family Lactobacillaceae) and is most closely related to Lactobacillus nodensis DSM 19682T (96.1 % similarity) and Lactobacillus tucceti KCTC 21005T (96.7 %). The results of DNA-DNA hybridization experiments demonstrated that strain NIBRBAC000499792T represents a novel species. Major fatty acids are C18 : 1ω9c, C16 : 0 and unidentified 18.846 and/or C19 : 1ω6c and/or C19 : 0cyclo. The predominant respiratory quinones are menaquinone-8 and menaquinone-9. The major polar lipids are phosphatidylglycerol and diphosphatidylglycerol. The minor polar lipids are one unidentified aminophospholipid, one unidentified phospholipid, and four unidentified lipids. Next-generation sequencing analysis of strain NIBRBAC000499792T indicated that the total genome size was 1 548 794 bp with a G+C content of 33.1 mol%, 1586 coding sequences, 50 tRNAs and nine rRNAs. The most closely related genomes belonged to Lactobacillus species. Most metabolic pathways were related to carbon metabolism and carbon fixation. Based on this polyphasic analysis, strain NIBRBAC000499792T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus terrae sp. nov. is proposed, with the type strain NIBRBAC000499792T (=KCTC 21093T=JCM 32269T).
['Kim|Hyun-Joong|HJ|', 'Lee|Hye Jin|HJ|', 'Lim|Bora|B|', 'Kim|Eiseul|E|', 'Kim|Hae-Yeong|HY|', 'Suh|Minhwan|M|', 'Hur|Moonsuk|M|']
D015373:Bacterial Typing Techniques D001482:Base Composition D004269:DNA, Bacterial D005227:Fatty Acids D007778:Lactobacillus D009693:Nucleic Acid Hybridization D010743:Phospholipids D010802:Phylogeny D012336:RNA, Ribosomal, 16S D056910:Republic of Korea D017422:Sequence Analysis, DNA D012988:Soil Microbiology D024482:Vitamin K 2
Lactobacillus novel species soil genome lactic acid bacteria