Genome-wide characterization of JASMONATE-ZIM DOMAIN transcription repressors in wheat (Triticum aestivum L.).
BACKGROUND
The JASMONATE-ZIM DOMAIN (JAZ) repressor family proteins are jasmonate co-receptors and transcriptional repressor in jasmonic acid (JA) signaling pathway, and they play important roles in regulating the growth and development of plants. Recently, more and more researches on JAZ gene family are reported in many plants. Although the genome sequencing of common wheat (Triticum aestivum L.) and its relatives is complete, our knowledge about this gene family remains vacant.


RESULTS
Fourteen JAZ genes were identified in the wheat genome. Structural analysis revealed that the TaJAZ proteins in wheat were as conserved as those in other plants, but had structural characteristics. By phylogenetic analysis, all JAZ proteins from wheat and other plants were clustered into 11 sub-groups (G1-G11), and TaJAZ proteins shared a high degree of similarity with some JAZ proteins from Aegliops tauschii, Brachypodium distachyon and Oryza sativa. The Ka/Ks ratios of TaJAZ genes ranged from 0.0016 to 0.6973, suggesting that the TaJAZ family had undergone purifying selection in wheat. Gene expression patterns obtained by quantitative real-time PCR (qRT-PCR) revealed differential temporal and spatial regulation of TaJAZ genes under multifarious abiotic stress treatments of high salinity, drought, cold and phytohormone. Among these, TaJAZ7, 8 and 12 were specifically expressed in the anther tissues of the thermosensitive genic male sterile (TGMS) wheat line BS366 and normal control wheat line Jing411. Compared with the gene expression patterns in the normal wheat line Jing411, TaJAZ7, 8 and 12 had different expression patterns in abnormally dehiscent anthers of BS366 at the heading stage 6, suggesting that specific up- or down-regulation of these genes might be associated with the abnormal anther dehiscence in TGMS wheat line.


CONCLUSION
This study analyzed the size and composition of the JAZ gene family in wheat, and investigated stress responsive and differential tissue-specific expression profiles of each TaJAZ gene in TGMS wheat line BS366. In addition, we isolated 3 TaJAZ genes that would be more likely to be involved in the regulation of abnormal anther dehiscence in TGMS wheat line. In conclusion, the results of this study contributed some novel and detailed information about JAZ gene family in wheat, and also provided 3 potential candidate genes for improving the TGMS wheat line.
['Wang|Yukun|Y|', 'Qiao|Linyi|L|', 'Bai|Jianfang|J|', 'Wang|Peng|P|', 'Duan|Wenjing|W|', 'Yuan|Shaohua|S|', 'Yuan|Guoliang|G|', 'Zhang|Fengting|F|', 'Zhang|Liping|L|0000-0003-1574-6254', 'Zhao|Changping|C|']
D000220:Adaptation, Biological D002874:Chromosome Mapping D016000:Cluster Analysis D019295:Computational Biology D019143:Evolution, Molecular D020869:Gene Expression Profiling D018506:Gene Expression Regulation, Plant D018745:Genome, Plant D055106:Genome-Wide Association Study D023281:Genomics D005810:Multigene Family D010802:Phylogeny D011401:Promoter Regions, Genetic D021381:Protein Transport D012045:Regulatory Sequences, Nucleic Acid D012097:Repressor Proteins D013312:Stress, Physiological D014908:Triticum
JASMONATE-ZIM DOMAIN Phylogenetic analysis Gene expression Anther dehiscence Thermo sensitive genic male sterile (TGMS)