Comprehensive portrait of cholesterol containing oxidized membrane.
Biological membranes are under significant oxidative stress caused by reactive oxygen species mostly originating during cellular respiration. Double bonds of the unsaturated lipids are most prone to oxidation, which might lead to shortening of the oxidized chain and inserting of terminal either aldehyde or carboxylic group. Structural rearrangement of oxidized lipids, addressed already, is mainly associated with looping back of the hydrophilic terminal group. This contribution utilizing dual-focus fluorescence correlation spectroscopy and electron paramagnetic resonance as well as atomistic molecular dynamics simulations focuses on the overall changes of the membrane structural and dynamical properties once it becomes oxidized. Particularly, attention is paid to cholesterol rearrangement in the oxidized membrane revealing its preferable interaction with carbonyls of the oxidized chains. In this view cholesterol seems to have a tendency to repair, rather than condense, the bilayer.
['Stefl|Martin|M|', 'Sachl|Radek|R|', 'Olżyńska|Agnieszka|A|', 'Amaro|Mariana|M|', 'Savchenko|Dariya|D|', 'Deyneka|Alexander|A|', 'Hermetter|Albin|A|', 'Cwiklik|Lukasz|L|', 'Humpolíčková|Jana|J|', 'Hof|Martin|M|']
D002462:Cell Membrane D002784:Cholesterol D057927:Hydrophobic and Hydrophilic Interactions D008051:Lipid Bilayers D008563:Membrane Lipids D056004:Molecular Dynamics Simulation D010084:Oxidation-Reduction D010743:Phospholipids
Oxidized lipids Fluorescence correlation spectroscopy Electron paramagnetic resonance Molecular dynamics simulations Lateral diffusion