Down-Regulation of ClC-3 Expression Reduces Epidermal Stem Cell Migration by Inhibiting Volume-Activated Chloride Currents.
ClC-3, a member of the ClC chloride (Cl(-)) channel family, has recently been proposed as the primary Cl(-) channel involved in cell volume regulation. Changes in cell volume influence excitability, contraction, migration, pathogen-host interactions, cell proliferation, and cell death processes. In this study, expression and function of ClC-3 channels were investigated during epidermal stem cell (ESC) migration. We observed differential expression of CLC-3 regulates migration of ESCs. Further, whole-cell patch-clamp recordings and image analysis demonstrated ClC-3 expression affected volume-activated Cl(-) current (I Cl,Vol) within ESCs. Live cell imaging systems, designed to observe cellular responses to overexpression and suppression of ClC-3 in real time, indicated ClC-3 may regulate ESC migratory dynamics. We employed IMARIS software to analyze the velocity and distance of ESC migration in vitro to demonstrate the function of ClC-3 channel in ESCs. As our data suggest volume-activated Cl(-) channels play a vital role in migration of ESCs, which contribute to skin repair by migrating from neighboring unwounded epidermis infundibulum, hair follicle or sebaceous glands, ClC-3 may represent a new and valuable target for stem cell therapies.
['Guo|Rui|R|', 'Pan|Fuqiang|F|', 'Tian|Yanping|Y|', 'Li|Hongli|H|', 'Li|Shirong|S|', 'Cao|Chuan|C|']
D000818:Animals D002460:Cell Line D002465:Cell Movement D018118:Chloride Channels D002712:Chlorides D000078404:Epidermal Cells D004817:Epidermis D015870:Gene Expression D005822:Genetic Vectors D006801:Humans D016086:Lentivirus D008297:Male D034622:RNA Interference D012333:RNA, Messenger D051381:Rats D013234:Stem Cells D014161:Transduction, Genetic
ClC-3 Epidermal stem cell Cell migration Volume-activated chloride currents