Interaction between fatty acid synthase and human epidermal growth receptor 2 (HER2) in osteosarcoma cells.
Elevated expression of fatty acid synthase (FASN) and human epidermal growth factor receptor-2 (HER2) are observed in human osteosarcoma (OS). The aim in this study is to investigate a possible connection between FASN expression and the activity of HER2. The immunohistochemistry staining was conducted on 24 OS specimens from patients, which revealed a significant positive correlation between FASN and HER2 as well as p-HER2 protein expression. Furthermore, the human OS cell lines MG-63 and U2-OS were treated with FASN-specific RNAi Plasmid or Lapatinib (an inhibitor of HER2). The mRNA of HER2 and FASN was measured using RT-PCR. Western blot was performed to detect the protein expression of HER2, p-HER2 and FASN. The results demonstrated that HER2 modulates FASN expression, inhibition of FASN resulted in down-regulation of HER2 and p-HER2 protein in OS cells. Our findings suggested that there was positive feedback regulation between FASN and HER2 expression and phosphorylation in OS cells.
['Long|Xin Hua|XH|', 'Zhao|Kai|K|', 'Zhang|Guo Mei|GM|', 'Zhou|Yang|Y|', 'Zhou|Rong Ping|RP|', 'Liu|Zhi Li|ZL|', 'Zhang|Zhi Hong|ZH|']
D015153:Blotting, Western D054890:Fatty Acid Synthase, Type I D015972:Gene Expression Regulation, Neoplastic D006801:Humans D007150:Immunohistochemistry D012516:Osteosarcoma D060888:Real-Time Polymerase Chain Reaction D018719:Receptor, ErbB-2
HER2 Osteosarcoma FASN positive feedback regulation