Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies.
Accurate and efficient demonstrations of protein localizations to the vacuole or tonoplast remain strict prerequisites to decipher the role of vacuoles in the whole plant cell biology and notably in defence processes. In this chapter, we describe a reliable procedure of protein subcellular localization study through transient transformations of Catharanthus roseus or onion cells and expression of fusions with fluorescent proteins allowing minimizing artefacts of targeting.
['Carqueijeiro|Inês|I|', 'Sepúlveda|Liuda J|LJ|', 'Mosquera|Angela|A|', 'Payne|Richard|R|', 'Corbin|Cyrielle|C|', 'Papon|Nicolas|N|', 'de Bernonville|Thomas Dugé|TD|', 'Besseau|Sébastien|S|', 'Lanoue|Arnaud|A|', 'Glévarec|Gaëlle|G|', 'Clastre|Marc|M|', 'St-Pierre|Benoit|B|', 'Atehortùa|Lucia|L|', "Giglioli-Guivarc'h|Nathalie|N|", "O'Connor|Sarah E|SE|", 'Oudin|Audrey|A|', 'Courdavault|Vincent|V|']
D001426:Bacterial Proteins D030008:Catharanthus D005822:Genetic Vectors D049452:Green Fluorescent Proteins D008164:Luminescent Proteins D008856:Microscopy, Fluorescence D019697:Onions D010940:Plant Proteins D021381:Protein Transport D011993:Recombinant Fusion Proteins D014170:Transformation, Genetic D014617:Vacuoles
Protein localization Vacuole Tonoplast Transient transformation Catharanthus roseus Allium cepa Biolistic GFP Onion peel Vacuolar targeting YFP