MiR-182-5p Knockdown Targeting PTEN Inhibits Cell Proliferation and Invasion of Breast Cancer Cells.
PURPOSE
Breast cancer (BC) is one of the most common malignant tumors, affecting a significant number of women worldwide. MicroRNAs (miRNAs) have been reported to play important roles in tumorigenesis. The aim of this study was to determine the roles of miR-182-5p in BC progression.


MATERIALS AND METHODS
The expressions of miR-182-5p and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) were measured in BC tissues and cells by quantitative real-time polymerase chain reaction or Western blot. Cell proliferation and invasion were detected by cell counting kit-8 assay and trans-well assay, respectively. The interaction between miR-182-5p and PTEN was probed by bioinformatics analysis, luciferase activity, and RNA immunoprecipitation. A murine xenograft model was established to investigate the role of miR-182-5p in BC progression in vivo.


RESULTS
An abundance of miR-182-5p was noted in BC tissues and cells. High expression of miR-182-5p was associated with poor survival. Abrogation of miR-182-5p inhibited cell proliferation and invasion in BC cells. Interestingly, PTEN was indicated as a target of miR-182-5p, and its restoration reversed miR-182-5p-mediated promotion of proliferation and invasion of BC cells. Moreover, depletion of miR-182-5p suppressed tumor growth via up-regulating PTEN expression in the murine xenograft model.


CONCLUSION
MiR-182-5p exhaustion blocked cell proliferation and invasion by regulating PTEN expression, providing a novel therapeutic avenue for treatment of BC.
['Zhao|Yue Sheng|YS|https://orcid.org/0000-0002-5438-3057', 'Yang|Wei Chao|WC|https://orcid.org/0000-0002-8552-9098', 'Xin|Hong Wei|HW|https://orcid.org/0000-0002-9708-8226', 'Han|Ji Xia|JX|https://orcid.org/0000-0003-4033-3718', 'Ma|Su Gang|SG|https://orcid.org/0000-0002-6406-6063']
D000328:Adult D000818:Animals D001483:Base Sequence D001943:Breast Neoplasms D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D005260:Female D015972:Gene Expression Regulation, Neoplastic D055785:Gene Knockdown Techniques D006801:Humans D008807:Mice, Inbred BALB C D008819:Mice, Nude D035683:MicroRNAs D009361:Neoplasm Invasiveness D051059:PTEN Phosphohydrolase D015854:Up-Regulation D023041:Xenograft Model Antitumor Assays
miR-182-5p PTEN proliferation invasion Breast cancer