Anti-inflammatory effects of soyasapogenol I-αa via downregulation of the MAPK signaling pathway in LPS-induced RAW 264.7 macrophages.
The crude extract of soyasaponins was reported to possess anti-inflammatory activity. We determined the new purity group I saponin, I-αa and I-γa that was isolated from wild soybean (Glycine soja) in terms of its efficacy in protecting RAW 264.7 macrophages from lipopolysaccharide (LPS)-stimuli. Cells were treated with soyasaponin I-αa/I-γa (30-300 μΜ) and LPS (0.1 μg/mL) for 24 h. Soyasaponin I-αa inhibited nitric oxide (NO) production at 100 μg/mL, while soyasaponin I-γa demonstrated this effect at a higher concentration (200 μg/mL). The expression levels of iNOS and COX-2 enzymes were downregulated by both soyasaponins. Soyasaponin I-αa exerted its effect via the TNF-α and IL-1β cytokines. However, soyasaponin I-γa only inhibited the expression of TNF-α. The inflammatory effect of group I soyasaponin was mainly mediated via the phosphorylation of the p38 and JNK proteins. Collectively, these results suggested the potential anti-inflammatory effects of soyasaponins.
['Yang|Seung Hwan|SH|', 'Le|Bao|B|', 'Androutsopoulos|Vasilis P|VP|', 'Tsukamoto|Chigen|C|', 'Shin|Tae-Sun|TS|', 'Tsatsakis|Aristides M|AM|', 'Chung|Gyuhwa|G|']
D000818:Animals D000893:Anti-Inflammatory Agents D051546:Cyclooxygenase 2 D015536:Down-Regulation D053583:Interleukin-1beta D008070:Lipopolysaccharides D020935:MAP Kinase Signaling System D008264:Macrophages D051379:Mice D009569:Nitric Oxide D052247:Nitric Oxide Synthase Type II D009828:Oleanolic Acid D010936:Plant Extracts D000067996:RAW 264.7 Cells D013025:Soybeans D014409:Tumor Necrosis Factor-alpha
Macrophage Soyasaponin Glycine soja Cytokines Inflammatory