Biomolecular Interactions of Tannin Isolated from Oenothera gigas with Liposomes.
We have examined the interaction between hydrolysable tannin 1-O-galloyl-4,6-hexahydroxydiphenoyl-β-D-glucose (OGβDG) with neutral liposomes as a model of cell membranes composed of three lipids: lecithin, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at different mass ratios. OGβDG in the concentration range 0.5-15 µg/ml (0.4-12 µM) strongly interacts with liposomal membranes by changing their structure, surface charge and fluidity. Used OGβDG molecules decrease and increase the rigidity of hydrophilic surface and hydrophobic parts of liposomes, respectively. At higher concentrations of tannin (>15 µM), liposomes are aggregated. Fourier Transform Infra-Red (FTIR) analysis showed that mainly -OH groups from OGβDG and also PO(2-) groups from phospholipids are responsible for the interaction. Obtained data indicate the importance of membrane lipid composition in interactions between tannins and cells.
['Sekowski|Szymon|S|', 'Ionov|Maksim|M|', 'Dubis|Alina|A|', 'Mavlyanov|Saidmukhtar|S|', 'Bryszewska|Maria|M|', 'Zamaraeva|Maria|M|']
D047348:Hydrolyzable Tannins D008051:Lipid Bilayers D008081:Liposomes D008560:Membrane Fluidity D015394:Molecular Structure D031664:Oenothera D010316:Particle Size D010743:Phospholipids D010936:Plant Extracts D017550:Spectroscopy, Fourier Transform Infrared D013634:Tannins
Tannins Liposomes Fluorescence Zeta potential