Nanofibrous yet injectable polycaprolactone-collagen bone tissue scaffold with osteoprogenitor cells and controlled release of bone morphogenetic protein-2.
In this work, we developed a nanofibrous, yet injectable orthobiologic tissue scaffold that is capable of hosting osteoprogenitor cells and controlling kinetic release profile of the encapsulated pro-osteogenic factor without diminishing its bioactivity over 21days. This innovative injectable scaffold was synthesized by incorporating electrospun and subsequently O2 plasma-functionalized polycaprolactone (PCL) nanofibers within the collagen type-I solution along with MC3T3-E1 cells (pre-osteoblasts) and bone morphogenetic protein-2 (BMP2). Through changing the PCL nanofiber concentration within the injectable scaffolds, we were able to tailor the mechanical strength, protein retention capacity, bioactivity preservation, and osteoinductive potential of the scaffolds. The nanofibrous internal structure of the scaffold allowed us to use a low dose of BMP2 (200ng/ml) to achieve osteoblastic differentiation in in vitro culture. The osteogenesis capacity of the injectable scaffolds were evaluated though measuring MC3T3-E1 cell proliferation, ALP activity, matrix mineralization, and early- and late-osteoblast specific gene expression profiles over 21days. The results demonstrated that the nanofibrous injectable scaffold provides not only an osteoinductive environment for osteoprogenitor cells to differentiate, but also a suitable biomechanical and biochemical environment to act as a reservoir for osteogenic factors with controlled release profile.
['Subramanian|Gayathri|G|', 'Bialorucki|Callan|C|', 'Yildirim-Ayan|Eda|E|']
D016475:3T3 Cells D000818:Animals D055396:Bone Morphogenetic Protein 2 D018786:Bone Substitutes D024042:Collagen Type I D003692:Delayed-Action Preparations D055119:Elastic Modulus D004867:Equipment Design D019544:Equipment Failure Analysis D007267:Injections D008422:Materials Testing D051379:Mice D057139:Nanofibers D010006:Osteoblasts D010012:Osteogenesis D011091:Polyesters D054457:Tissue Scaffolds D014783:Viscosity
Injectable scaffold Polycaprolactone Collagen type-I BMP-2 release Osteogenic differentiation