The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells.
The infectious hematopoietic necrosis virus (IHNV; Rhabdoviridae, Novirhabdovirus) infects teleost fish, such as salmon and trout, and is responsible for significant losses in the aquaculture industry and in wild fish populations. Although IHNV enters the host through the skin at the base of the fins, the viral adhesion and entry mechanisms are not fully understood. In recent years, evidence has accumulated in support of the key roles played by protein-carbohydrate interactions between host lectins secreted to the extracellular space and virion envelope glycoproteins in modulating viral adhesion and infectivity. In this study, we assessed in vitro the potential role(s) of zebrafish (Danio rerio) proto type galectin-1 (Drgal1-L2) and a chimera galectin-3 (Drgal3-L1) in IHNV adhesion to epithelial cells. Our results suggest that the extracellular Drgal1-L2 and Drgal3-L1 interact directly and in a carbohydrate-dependent manner with the IHNV glycosylated envelope and glycans on the epithelial cell surface, significantly reducing viral adhesion.
['Nita-Lazar|Mihai|M|', 'Mancini|Justin|J|', 'Feng|Chiguang|C|', 'González-Montalbán|Núria|N|', 'Ravindran|Chinnarajan|C|', 'Jackson|Shawn|S|', 'de Las Heras-Sánchez|Ana|A|', 'Giomarelli|Barbara|B|', 'Ahmed|Hafiz|H|', 'Haslam|Stuart M|SM|', 'Wu|Gang|G|', 'Dell|Anne|A|', 'Ammayappan|Arun|A|', 'Vakharia|Vikram N|VN|', 'Vasta|Gerardo R|GR|']
D000818:Animals D002478:Cells, Cultured D004847:Epithelial Cells D037161:Galectins D054884:Host-Pathogen Interactions D029142:Infectious hematopoietic necrosis virus D011993:Recombinant Fusion Proteins D018353:Rhabdoviridae Infections D014759:Viral Envelope Proteins D014774:Virulence D053585:Virus Attachment D015027:Zebrafish D029961:Zebrafish Proteins
Zebrafish Galectin Infectious hematopoietic necrosis virus (IHNV) Viral adhesion Epithelial cells IHNV envelope glycoprotein