Application of ISSR-PCR for rapid strain typing of Debaryomyces hansenii isolated from dry-cured Iberian ham.
Yeast populations of dry-cured Iberian ham isolated from seven industries in the province of Badajoz were characterized by ISSR-PCR using the (CAG)4 primer and PCR-RFLP of the ITS1-5.8S rRNA-ITS2 fragment, and identified by DNA sequencing. A total of 242 isolates were analyzed, indicating the primary species present was Debaryomyces hansenii at 80.9% of the isolates followed by Candida zeylanoides at 10.3% of the isolates. The remainders of isolates were identified as Yamadazyma triangularis, Sporobolomyces roseus, Meyerozyma guilliermondii, Rhodotorula slooffiae, and Cryptococcus victoriae. The ISSR-PCR method was a fast and reliable method which was able to discriminate species at a level comparable to restriction analyses of the ITS1-5.8S rRNA-ITS2 region. This method allowed for strain typing of D. hansenii, yielding 29 different PCR patterns within 196 isolates. Moreover, ISSR-PCR using the (CAG)4 primer indicated that this technique could be a promising tool for rapid discrimination of yeast starter cultures and spoilage species in dry-cured Iberian ham.
['Gallardo|G|G|', 'Ruiz-Moyano|S|S|', 'Hernández|A|A|', 'Benito|M J|MJ|', 'Córdoba|M G|MG|', 'Pérez-Nevado|F|F|', 'Martín|A|A|']
D000818:Animals D017931:DNA Primers D004271:DNA, Fungal D008461:Meat Products D016533:Mycological Typing Techniques D016133:Polymerase Chain Reaction D012091:Repetitive Sequences, Nucleic Acid D004718:Saccharomycetales D013552:Swine
ISSR-PCR Strain typing Dry-cured Iberian ham Yeast Identification RFLP of ITS-5.8S rRNA