Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis.
The aquatic habitats of the Amazon basin present dramatic variation of oxygen level, and, to survive such changes, many aquatic animals developed biochemical and physiological adaptations. The advanced teleost Astronotus crassipinnis (Perciformes) is a fish tolerant to hypoxia and known to endure such naturally variable environments. Hypoxia-Inducible factor-1α (hif-1α) is among the most important and studied genes related to hypoxia-tolerance, maintaining regular cellular function and controlling anaerobic metabolism. In the present work, we studied hif-1α expression and related it to changes in metabolic pathways of Astronotus crassipinnis exposed to 1, 3 and 5 h of hypoxia, followed by 3 h of recovery. The results show that A. crassipinnis depresses aerobic metabolic under hypoxia, with a decrease in glycolysis and oxidative enzyme activities, and increases its anaerobic metabolism with an increase in LDH activity coupled with a decrease in oxygen consumption, which indicates an increase in anaerobic capacity. In addition, the animal differentially regulates hif-1α gene in each tissue studied, with a positive relationship to its metabolic profile, suggesting that hif-1α might be one of the most important induction factors that regulate hypoxia tolerance in this species.
['Heinrichs-Caldas|Waldir|W|', 'Campos|Derek Felipe|DF|', 'Paula-Silva|Maria Nazaré|MN|', 'Almeida-Val|Vera Maria Fonseca|VMF|']
D000222:Adaptation, Physiological D000818:Animals D017756:Aquaculture D015687:Cell Hypoxia D023681:Cichlids D004734:Energy Metabolism D005260:Female D029941:Fish Proteins D018507:Gene Expression Regulation, Developmental D006019:Glycolysis D051795:Hypoxia-Inducible Factor 1, alpha Subunit D045022:Lactate Dehydrogenases D008099:Liver D008297:Male D008954:Models, Biological D018482:Muscle, Skeletal D009928:Organ Specificity D010101:Oxygen Consumption D011897:Random Allocation D013997:Time Factors
Hypoxia hif-1α expression Enzyme activity Amazon - Astronotus crassipinnis Respirometry