Magnetic Nanocomposite Hydrogel for Potential Cartilage Tissue Engineering: Synthesis, Characterization, and Cytocompatibility with Bone Marrow Derived Mesenchymal Stem Cells.
Hydrogels possess high water content and closely mimic the microenvironment of extracellular matrix. In this study, we created a hybrid hydrogel containing type II collagen, hyaluronic acid (HA), and polyethylene glycol (PEG) and incorporated magnetic nanoparticles into the hybrid hydrogels of type II collagen-HA-PEG to produce a magnetic nanocomposite hydrogel (MagGel) for cartilage tissue engineering. The results showed that both the MagGel and hybrid gel (Gel) were successfully cross-linked and the MagGel responded to an external magnet while maintaining structural integrity. That is, the MagGel could travel to the tissue defect sites in physiological fluids under remote magnetic guidance. The adhesion density of bone marrow derived mesenchymal stem cells (BMSCs) on the MagGel group in vitro was similar to the control group and greater than the Gel group. The morphology of BMSCs was normal and consistent in all groups. We also found that BMSCs engulfed magnetic nanoparticles in culture and the presence of magnetic nanoparticles did not affect BMSC adhesion and morphology. We hypothesized that the ingested nanoparticles may be eventually broken down by lysosome and excreted through exocytosis; further studies are necessary to confirm this. This study reports a promising magnetic responsive nanocomposite hydrogel for potential cartilage tissue engineering applications, which should be further studied for its effects on cell functions when combined with electromagnetic stimulation.
['Zhang|Naiyin|N|', 'Lock|Jaclyn|J|', 'Sallee|Amy|A|', 'Liu|Huinan|H|']
D000818:Animals D001672:Biocompatible Materials D002358:Cartilage, Articular D002448:Cell Adhesion D002452:Cell Count D048430:Cell Shape D002478:Cells, Cultured D003094:Collagen D005782:Gels D020136:Hydrogel, Polyethylene Glycol Dimethacrylate D060328:Magnetic Phenomena D059630:Mesenchymal Stem Cells D053761:Nanocomposites D017207:Rats, Sprague-Dawley D012756:Sheep D012996:Solutions D013052:Spectrometry, X-Ray Emission D017550:Spectroscopy, Fourier Transform Infrared D023822:Tissue Engineering
cartilage tissue engineering cytocompatibility bone marrow derived mesenchymal stem cells magnetic nanoparticles magnetic nanocomposite hydrogel (MagGel) hybrid gel