Physiological and biochemical characteristics of laboratory induced mutants of Botrytis cinerea with resistance to fluazinam.
Botrytis cinerea is a necrotrophic and filamentous fungus with a high risk of developing resistance to fungicides. The pyridinamine fungicide fluazinam has been reported to have excellent activity against B. cinerea and better effect on controlling gray mold. In this study, the physiological and biochemical characteristics of laboratory-induced mutants of B. cinerea with resistance to fluazinam has been investigated. Compared to the wild-type strains, the fluazinam-resistant mutants had a significant decrease in respiratory rate, glycerol, oxalate, and ATP contents, and an increase in ATPase activity and sensitivity to osmotic pressure, but did not differ in cell membrane permeability. Sequencing indicated that two parental strains and four resistant mutants were identical in the nucleotide sequence of F-ATPase gene. These results will enrich our understanding of the resistance mechanism of B. cinerea to fluazinam.
['Shao|Wenyong|W|', 'Zhang|Yu|Y|', 'Ren|Weichao|W|', 'Chen|Changjun|C|']
D000251:Adenosine Triphosphatases D000255:Adenosine Triphosphate D000631:Aminopyridines D001483:Base Sequence D020171:Botrytis D002463:Cell Membrane Permeability D004271:DNA, Fungal D025141:Drug Resistance, Fungal D005656:Fungal Proteins D005659:Fungicides, Industrial D005947:Glucose D005990:Glycerol D009154:Mutation D019815:Oxalic Acid D017422:Sequence Analysis, DNA
Characteristics Botrytis cinerea Resistance Fluazinam