Mechanism of deoxynivalenol-induced neurotoxicity in weaned piglets is linked to lipid peroxidation, dampened neurotransmitter levels, and interference with calcium signaling.
Deoxynivalenol（DON） has broad toxicity in livestock, but we know little about its neurotoxic mechanisms. We investigated DON neurotoxicity in the cerebral cortex, cerebellum, and hippocampus of "Duroc × Landrace × Yokshire" piglets. Control piglets were fed a basal diet, while those in low- and high-treatment groups were fed diets with 1.3 mg/kg and 2.2 mg/kg DON, respectively. After a 60 d trial, scanning electron microscopy revealed the destruction of hippocampal cell ultrastructure. As DON concentrations increased, oxidative damage also increased in the cerebral cortex, cerebellum, and hippocampus. Norepinephrine and 5-hydroxytryptamine concentrations tended to increase, whereas dopamine and γ-aminobutyric acid concentrations decreased. We also observed an increase in calcium concentration, relative mRNA expression of calcium/calmodulin-dependent protein kinase II (CaMKII), and CaMKII phosphorylation. However, calmodulin (CaM) mRNA and protein content decreased. Overall, our results suggest that DON acts through the Ca2+/CaM/CaMKII signaling pathway to influence cerebral lipid peroxidation and neurotransmitter levels.
['Wang|Xichun|X|', 'Chen|Xiaofang|X|', 'Cao|Li|L|', 'Zhu|Lei|L|', 'Zhang|Yafei|Y|', 'Chu|Xiaoyan|X|', 'Zhu|Dianfeng|D|', 'Rahman|Sajid Ur|SU|', 'Peng|Chenglu|C|', 'Feng|Shibin|S|', 'Li|Yu|Y|', 'Wu|Jinjie|J|']
D000818:Animals D001921:Brain D002118:Calcium D020013:Calcium Signaling D004032:Diet D015227:Lipid Peroxidation D008297:Male D020258:Neurotoxicity Syndromes D018377:Neurotransmitter Agents D010084:Oxidation-Reduction D013552:Swine D014255:Trichothecenes D014886:Weaning
Neurotoxicity Piglets Lipid peroxidation Neurotransmitter Deoxynivalenol