Monitoring genotoxicity in freshwater microcrustaceans: A new application of the micronucleus assay.
We have applied the micronucleus (MN) assay to the measurement of genotoxicity in microcrustaceans. Daphnids (Daphnia magna) and Copepods (Acanthocyclops robustus) were collected in situ and acclimated in the lab for 24h. The MN assay was successful with the Daphnids but not with the Copepods. Adult Daphnids were exposed to sublethal concentrations of metals (Cu, Zn, Cd) or insecticide (deltamethrin) for 2 and 7d. Dose-dependent induction of MN was observed after 2 d exposure, with 2-fold induction at the highest doses for each chemical tested. The advantages and ecological relevance of using Daphnids in genotoxicity assessment are highlighted. The Daphnid assay may be a reliable test for aquatic genotoxicity hazard/risk assessment and a useful alternative to studies of amphibians.
['Barka|S|S|', 'Ouanes|Z|Z|', 'Gharbi|A|A|', 'Gdara|I|I|', 'Mouelhi|S|S|', 'Hamza-Chaffai|A|A|']
D000818:Animals D002104:Cadmium D003300:Copper D003621:Daphnia D015162:Micronucleus Tests D009152:Mutagenicity Tests D009570:Nitriles D011722:Pyrethrins D015032:Zinc D015048:Zooplankton
Micronucleus assay Daphnia magna Metals Deltamethrin Zooplankton