Frizzled10 mediates WNT1 and WNT3A signaling in the dorsal spinal cord of the developing chick embryo.
BACKGROUND
WNT1 and WNT3A drive a dorsal to ventral gradient of β-catenin-dependent Wnt signaling in the developing spinal cord. However, the identity of the receptors mediating downstream functions remains poorly understood.


RESULTS
In this report, we show that the spatiotemporal expression patterns of FZD10 and WNT1/WNT3A are highly correlated. We further show that in the presence of LRP6, FZD10 promotes WNT1 and WNT3A signaling using an 8xSuperTopFlash reporter assay. Consistent with a functional role for FZD10, we demonstrate that FZD10 is required for proliferation in the spinal cord. Finally, by using an in situ proximity ligation assay, we observe an interaction between FZD10 and WNT1 and WNT3A proteins.


CONCLUSIONS
Together, our results identify FZD10 as a receptor for WNT1 and WNT3A in the developing chick spinal cord.
['Galli|Lisa M|LM|', 'Munji|Roeben N|RN|', 'Chapman|Susan C|SC|', 'Easton|Ann|A|', 'Li|Lydia|L|', 'Onguka|Ouma|O|', 'Ramahi|Joseph S|JS|', 'Suriben|Rowena|R|', 'Szabo|Linda A|LA|', 'Teng|Camilla|C|', 'Tran|Baouyen|B|', 'Hannoush|Rami N|RN|', 'Burrus|Laura W|LW|']
D000818:Animals D030161:Avian Proteins D002642:Chick Embryo D051157:Frizzled Receptors D013116:Spinal Cord D051155:Wnt1 Protein D060509:Wnt3A Protein
WNT1 WNT3A chick FZD10 LRP6 palmitate