Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical Applications.
Hydrogel with chemical modification has been used for 3D printing in the biomedical field of cell and tissue-based regeneration because it provides a good cellular microenvironment and mechanical supportive ability. As a scaffold and a matrix, hydrogel itself has to be modified chemically and physically to form a β-sheet crosslinking structure for the strength of the biomaterials. These chemical modifications could affect the biological damage done to encapsulated cells or surrounding tissues due to unreacted chemical residues. Biological assessment, including assessment of the cytocompatibility of hydrogel in clinical trials, must involve testing with cytotoxicity, irritation, and sensitization. Here, we modified silk fibroin and glycidyl methacrylate (Silk-GMA) and evaluated the physical characterizations, residual chemical detection, and the biological effect of residual GMA depending on dialysis periods. Silk-GMA depending on each dialysis period had a typical β-sheet structure in the characterization analysis and residual GMA decreased from dialysis day 1. Moreover, cell proliferation and viability rate gradually increased; additionally, necrotic and apoptotic cells decreased from dialysis day 2. These results indicate that the dialysis periods during chemical modification of natural polymer are important for removing unreacted chemical residues and for the potential application of the manufacturing standardization for chemically modified hydrogel for the clinical transplantation for tissue engineering and biomedical applications.
['Hong|Heesun|H|0000-0002-9301-4107', 'Lee|Ok Joo|OJ|', 'Lee|Young Jin|YJ|', 'Lee|Ji Seung|JS|', 'Ajiteru|Olatunji|O|0000-0002-4924-1820', 'Lee|Hanna|H|', 'Suh|Ye Ji|YJ|', 'Sultan|Md Tipu|MT|', 'Kim|Soon Hee|SH|', 'Park|Chan Hum|CH|']
D000818:Animals D001672:Biocompatible Materials D012831:Bombyx D002470:Cell Survival D003956:Dialysis D004852:Epoxy Compounds D005349:Fibroins D008422:Materials Testing D008689:Methacrylates D051379:Mice D041681:NIH 3T3 Cells D023822:Tissue Engineering
silk fibroin glycidyl methacrylate tissue engineering chemical modification dialysis periods cytocompatiblity