Effects of CoQ10 on the quality of ram sperm during cryopreservation in plant and animal based extenders.
The purpose of this study was to evaluate the effect of different concentrations of CoQ10 in soybean lecithin (SL) or egg yolk (EY) extenders on ram semen cryopreservation. Semen samples were collected from five rams, twice a week, then diluted in the extenders (SL and EY) containing different concentrations of CoQ10 as follows: extender containing SL: 0 μM (control, SL/Q0), 1 μM (SL/Q1), 2 μM (SL/Q2), 5 μM (SL/Q5) and 10 μM (SL/Q10) CoQ10; extender containing EY: 0 μM (control, EY/Q0), 1 μM (EY/Q1), 2 μM (EY/Q2), 5 μM (EY/Q5) and 10 μM (EY/Q10) CoQ10. Sperm motion characteristics, membrane integrity, abnormal morphology, viability, apoptotic-like changes, mitochondria active potential, acrosome integrity and lipid peroxidation were evaluated after freeze-thaw process. The SL/Q1, SL/Q2, EY/Q1 and EY/Q2 resulted in greater (P ≤ 0.05) sperm total motility, progressive motility, membrane integrity and mitochondria active potential compared to the other groups. Acrosome integrity in the SL/Q0, SL/Q1, SL/Q2, EY/Q0, EY/Q1 and EY/Q2 groups was greater (P ≤ 0.05) than in the SL/Q5, SL/Q10, EY/Q5 and EY/Q10 groups. The SL/Q2 and EY/Q2 treatment groups had greater (P ≤ 0.05) sperm viability rates and less apoptotic-like changes and lipid peroxidation. The CoQ10 compound could be explored as a novel potential antioxidant for cryopreservation of ram semen because with used of this compound in the present study there was an improved post-thawed sperm quality.
['Masoudi|Reza|R|', 'Sharafi|Mohsen|M|', 'Shahneh|Ahmad Zare|AZ|']
D000818:Animals D000975:Antioxidants D015925:Cryopreservation D004530:Egg Yolk D005615:Freezing D054709:Lecithins D015227:Lipid Peroxidation D008297:Male D012661:Semen D012662:Semen Preservation D012756:Sheep D013094:Spermatozoa D014451:Ubiquinone
Ram Soybean lecithin Coenzyme Q10 Sperm freezing