Peptidomic analysis of cartilage and subchondral bone in OA patients.
BACKGROUND
The objective of this study was to develop a method for directly analysing osteochondral samples straight out of the operating room without cell culturing, thereby enabling identification of potential peptide biomarkers to better understand the mechanisms involved in the development of osteoarthritis and pain.


MATERIAL AND METHODS
Osteochondral plugs from wounded and macroscopically nonwounded zones of the femur condyle were collected from six patients with manifest osteoarthritis (OA) undergoing total knee arthroplasty (TKA). The samples were demineralized and supernatant was collected and isotopically marked with Tandem Mass Tag (TMT) labelling and analysed using liquid chromatography coupled with tandem mass spectrometry LC-MS/MS.


RESULTS
Using peptidomics, 6292 endogenous peptides were identified. Five hundred sixty-six peptides (8 identified endogenous peptides) differed significantly (P-value 0.10) from wounded zones compared to nonwounded zones.


CONCLUSION
This pilot study shows promising results for enabling peptidomic analysis of cartilage and bone straight out of the operating room. With further refinement, peptidomics can potentially become a diagnostic tool for OA, and improve the knowledge of disease progression and genesis of pain.
['Gatenholm|Birgitta|B|https://orcid.org/0000-0002-7204-9343', 'Gobom|Johan|J|', 'Skillbäck|Tobias|T|', 'Blennow|Kaj|K|', 'Zetterberg|Henrik|H|', 'Brittberg|Mats|M|']
D015415:Biomarkers D002356:Cartilage D002853:Chromatography, Liquid D005269:Femur D006801:Humans D020370:Osteoarthritis, Knee D010455:Peptides D010865:Pilot Projects D013048:Specimen Handling D053719:Tandem Mass Spectrometry
cartilage osteoarthritis pain mass spectrometry neuropeptides