Low-Temperature Storage Improves the Over-Time Stability of Implantable Glucose and Lactate Biosensors.
Molecular biomarkers are very important in biology, biotechnology and even in medicine, but it is quite hard to convert biology-related signals into measurable data. For this purpose, amperometric biosensors have proven to be particularly suitable because of their specificity and sensitivity. The operation and shelf stability of the biosensor are quite important features, and storage procedures therefore play an important role in preserving the performance of the biosensors. In the present study two different designs for both glucose and lactate biosensor, differing only in regards to the containment net, represented by polyurethane or glutharaldehyde, were studied under different storage conditions (+4, -20 and -80 °C) and monitored over a period of 120 days, in order to evaluate the variations of kinetic parameters, as VMAX and KM, and LRS as the analytical parameter. Surprisingly, the storage at -80 °C yielded the best results because of an unexpected and, most of all, long-lasting increase of VMAX and LRS, denoting an interesting improvement in enzyme performances and stability over time. The present study aimed to also evaluate the impact of a short-period storage in dry ice on biosensor performances, in order to simulate a hypothetical preparation-conservation-shipment condition.
['Puggioni|Giulia|G|', 'Calia|Giammario|G|', 'Arrigo|Paola|P|', 'Bacciu|Andrea|A|', 'Bazzu|Gianfranco|G|', 'Migheli|Rossana|R|', 'Fancello|Silvia|S|', 'Serra|Pier Andrea|PA|', 'Rocchitta|Gaia|G|0000-0002-4319-4197']
D015374:Biosensing Techniques D003080:Cold Temperature D004367:Dry Ice D004867:Equipment Design D005947:Glucose D007700:Kinetics D019344:Lactic Acid D011309:Preservation, Biological D013997:Time Factors
low-temperature storage over-time stability glucose lactate biosensors