Installation of a Rigid EDTA-Like Motif into a Protein α-Helix for Paramagnetic NMR Spectroscopy with Cobalt(II) Ions.
Coupling two copies of an iminodiacetic acid-cysteine hybrid ligand to a pair of cysteine residues positioned in an i, i+4 arrangement within a protein α-helix leads to generation of an EDTA-like metal ion-binding motif. Rigid binding of a Co(II) ion by this motif produces pseudo-contact shifts suitable for paramagnetic NMR structural studies.
['Swarbrick|James D|JD|http://orcid.org/0000-0002-0238-7929', 'Ung|Phuc|P|', 'Dennis|Matthew L|ML|', 'Lee|Michael D|MD|', 'Chhabra|Sandeep|S|', 'Graham|Bim|B|']
D000595:Amino Acid Sequence D001665:Binding Sites D003035:Cobalt D004492:Edetic Acid D008024:Ligands D009682:Magnetic Resonance Spectroscopy D011485:Protein Binding
paramagnetic NMR spectroscopy pseudo-contact shifts alpha-helix cobalt protein structures