Quantitative Analysis of Southern rice black-streaked dwarf virus in Sogatella furcifera and Virus Threshold for Transmission.
We investigated Southern rice black-streaked dwarf virus (SRBSDV) accumulation in a vector insect, the whitebacked planthopper (Sogatella furcifera), to elucidate the association of virus accumulation in the vector with virus transmission efficiency. Real-time quantitative reverse transcription polymerase chain reaction analysis confirmed that this virus is transmitted in a persistent-propagative manner. SRBSDV was successfully transmitted by S. furcifera males in which RNA accumulation of the capsid protein gene of SRBSDV was >10(3) in the whole body of S. furcifera, indicating that the threshold accumulation of the virus RNA for virus transmission is 10(3) in an S. furcifera male. The SRBSDV detection rate in the immigrant population of S. furcifera was high in 2011 (39.5%); however, most of the insects contained fewer than 10(3) RNAs of the capsid protein gene. This result indicates that the risk of SRBSDV epidemics could be estimated from the proportion of virus-transmissible S. furcifera (i.e., S. furcifera that contained more than 10(3) RNAs of the virus capsid protein gene) rather than the SRBSDV detection rate in S. furcifera.
['Matsukura|Keiichiro|K|', 'Towata|Tomomi|T|', 'Yoshida|Kazuhiro|K|', 'Sakai|Junichi|J|', 'Okuda|Mitsuru|M|', 'Onuki|Masatoshi|M|', 'Matsumura|Masaya|M|']
D000818:Animals D036022:Capsid Proteins D005260:Female D006430:Hemiptera D007303:Insect Vectors D008297:Male D012275:Oryza D010935:Plant Diseases D012367:RNA, Viral D060888:Real-Time Polymerase Chain Reaction D012087:Reoviridae D014764:Viral Proteins
overseas immigration reovirus stunted growth viral disease