Evidence for Nanoparticle-Induced Lysosomal Dysfunction in Lung Adenocarcinoma (A549) Cells.
BACKGROUND
Polystyrene nanoparticles (PNP) are taken up by primary rat alveolar epithelial cell monolayers (RAECM) in a time-, dose-, and size-dependent manner without involving endocytosis. Internalized PNP in RAECM activate autophagy, are delivered to lysosomes, and undergo [Ca2+]-dependent exocytosis. In this study, we explored nanoparticle (NP) interactions with A549 cells.


METHODS
After exposure to PNP or ambient pollution particles (PM0.2), live single A549 cells were studied using confocal laser scanning microscopy. PNP uptake and egress were investigated and activation of autophagy was confirmed by immunolabeling with LC3-II and LC3-GFP transduction/colocalization with PNP. Mitochondrial membrane potential, mitophagy, and lysosomal membrane permeability (LMP) were assessed in the presence/absence of apical nanoparticle (NP) exposure.


RESULTS
PNP uptake into A549 cells decreased in the presence of cytochalasin D, an inhibitor of macropinocytosis. PNP egress was not affected by increased cytosolic [Ca2+]. Autophagy activation was indicated by increased LC3 expression and LC3-GFP colocalization with PNP. Increased LMP was observed following PNP or PM0.2 exposure. Mitochondrial membrane potential was unchanged and mitophagy was not detected after NP exposure.


CONCLUSIONS
Interactions between NP and A549 cells involve complex cellular processes leading to lysosomal dysfunction, which may provide opportunities for improved nanoparticle-based therapeutic approaches to lung cancer management.
['Sipos|Arnold|A|', 'Kim|Kwang-Jin|KJ|0000-0001-7547-1681', 'Sioutas|Constantinos|C|0000-0001-5146-0857', 'Crandall|Edward D|ED|']
D000077192:Adenocarcinoma of Lung D001343:Autophagy D045744:Cell Line, Tumor D006801:Humans D008175:Lung Neoplasms D008247:Lysosomes D053758:Nanoparticles D010873:Pinocytosis D011137:Polystyrenes
lysosome autophagy lysosomal membrane permeability mitochondria pneumocyte