Association of Complement Factor D and H Polymorphisms with Recurrent Pregnancy Loss.
Recurrent pregnancy loss (RPL) is defined as two or more consecutive pregnancy losses prior to 20 weeks of gestation, and the incidence of RPL is estimated at 1% of all pregnancies. While the etiologies of RPL are diverse, immune function is considered to be an important cause of RPL. In particular, the complement system is essential for stable development of the placenta and fetus. Moreover, complement factor D (CFD) and complement factor H (CFH) are important regulators of the complement system and are associated with diseases, such as age-related macular degeneration. Therefore, we investigated whether polymorphisms of CFD and CFH are associated with RPL in 412 women with RPL and 384 control women. Genotyping of three polymorphisms (CFD rs2230216, CFH rs1065489, and CFH rs1061170) was performed by TaqMan probe real-time PCR and PCR-restriction fragment length polymorphism. Association of three polymorphisms with RPL was evaluated by statistical analysis. The GT/TC genotype combination of CFH rs1065489 G>T/CFH rs1061170 T>C was associated with a decreased risk of RPL occurrence compared with reference genotypes (adjusted odds ratio [AOR] = 0.439; 95% confidence interval [CI] = 0.238-0.810; p = 0.008), and this association remained significant after adjustment for multiple comparisons using false discovery rate (FDR) correction (p = 0.040). In addition, the CFH rs1065489G>T polymorphism is associated with homocysteine and prolactin level and CFH rs1061170 TC genotype is related to uric acid and triglycerides level in RPL patients. Therefore, those factors could be possible clinical risk factors in RPL patients.
['Cho|Hee Young|HY|', 'Park|Han Sung|HS|', 'Ko|Eun Ju|EJ|', 'Ryu|Chang Soo|CS|', 'Kim|Jung Oh|JO|', 'Kim|Young Ran|YR|', 'Ahn|Eun Hee|EH|', 'Lee|Woo Sik|WS|', 'Kim|Nam Keun|NK|']
D000026:Abortion, Habitual D000328:Adult D000483:Alleles D016022:Case-Control Studies D011416:Complement Factor D D017242:Complement Factor H D005787:Gene Frequency D056726:Genetic Association Studies D020022:Genetic Predisposition to Disease D005838:Genotype D006801:Humans D011110:Polymorphism, Genetic
complement factor D polymorphism recurrent pregnancy loss complement factor H