Alpha-Hederin Nanopore for Single Nucleotide Discrimination.
Various types of biological and synthetic nanopores have been developed and utilized for the high-throughput investigation of individual biomolecules. Biological nanopores made with channel proteins are so far superior to solid-state ones in terms of sensitivity and reproducibility. However, the performance of a biological nanopore is dependent on the protein in the channel structure its dimensions are predetermined and are difficult to modify for broader applications. Here inspired by the cytotoxic mechanisms of a saponin derivative, alpha-hederin, we report a nonproteinaceous nanopore that can be formed spontaneously in a lipid membrane. We propose the pore-forming mechanism of alpha-hederin in a cholesterol-rich lipid membrane and a strategy to control the pore-forming rate by a lipid partitioning method. The small diameter and effective thickness of alpha-hederin nanopores enabled us to discriminate ssDNA homopolymers as well as four types of nucleotides, showing its potential as a DNA sequencing tool.
['Jeong|Ki-Baek|KB|', 'Luo|Ke|K|', 'Lee|Hwankyu|H|0000-0002-2036-1490', 'Lim|Min-Cheol|MC|', 'Yu|Jin|J|', 'Choi|Soo-Jin|SJ|0000-0001-5131-8156', 'Kim|Ki-Bum|KB|0000-0003-1392-5021', 'Jeon|Tae-Joon|TJ|0000-0002-4882-9040', 'Kim|Young-Rok|YR|0000-0001-6448-6079']
D056004:Molecular Dynamics Simulation D058608:Nanopores D036103:Nanotechnology D009711:Nucleotides D009828:Oleanolic Acid D011108:Polymers D012503:Saponins
alpha-hederin nanopore single nucleotide discrimination self-assembly size-tunable