Polygonatum cyrtonema Hua polysaccharide exhibits anti-fatigue activity via regulating osteocalcin signaling.
In the present study, we explored the anti-fatigue activity and its potential mechanism of a purified Polygonatum cyrtonema polysaccharide (PCP) on mice using weight-loaded swimming test. Results showed that PCP remarkably prolonged the exhaustive swimming time of mice when compared with normal control group. Meanwhile, PCP decreased serum levels of lactic acid (LA), blood uric nitrogen (BUN), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA), and increased the contents of liver glycogen, muscle glycogen and muscle ATP. These results revealed that PCP had good anti-fatigue ability. The histomorphologic analysis showed that PCP increased the cross-section area of the muscle fibers. Furthermore, PCP significantly enhanced the protein levels of bone morphogenetic protein-2 (BMP-2), phosphor-Smad1, Runt-related transcription factor 2 (Runx2) and osteocalcin (OC) in skeleton. Similar variation was also observed in the expression of osteocalcin signaling mediators of phosphorylated cAMP-response element binding protein (p-CREB) and phosphorylated hormone-sensitive lipase (p-HSL) in skeletal muscle. These results suggested that PCP resisted fatigue possibly via regulating osteocalcin signaling.
['Shen|Wen-Di|WD|', 'Li|Xue-Ying|XY|', 'Deng|Yuan-Yuan|YY|', 'Zha|Xue-Qiang|XQ|', 'Pan|Li-Hua|LH|', 'Li|Qiang-Ming|QM|', 'Luo|Jian-Ping|JP|']
D000818:Animals D001835:Body Weight D002374:Catalase D005221:Fatigue D005979:Glutathione Peroxidase D006003:Glycogen D008297:Male D008315:Malondialdehyde D051379:Mice D008810:Mice, Inbred C57BL D018482:Muscle, Skeletal D010936:Plant Extracts D031384:Polygonatum D011134:Polysaccharides D015398:Signal Transduction D013482:Superoxide Dismutase
Polygonatum cyrtonema Polysaccharide Anti-fatigue Osteocalcin