Detection of a mecC-positive Staphylococcus saprophyticus from bovine mastitis in Argentina.
INTRODUCTION
Bovine mastitis causes important economic losses in the dairy industry. Coagulase-negative staphylococci (CNS) are a group of bacteria commonly isolated from bovine mastitis and can display resistance to a wide range of antimicrobial agents.


OBJECTIVES
The objective of this study was to determine staphylococcal resistance towards β-lactam, macrolide and lincosamide antimicrobials in quarters previously treated with third-generation cephalosporin and after lincosamide intramammary therapy.


METHODS
Sick quarters of eighteen cows from Villaguay, Entre Ríos (Argentina) with clinical mastitis were studied. All staphylococcal isolates were tested by disk diffusion for their antimicrobial susceptibilities. Cefoxitin resistance was investigated by PCR and sequencing for both the mecA and mecC genes.


RESULTS
Resistances to penicillin, oxacillin and cefoxitin were observed, whereas no resistance to macrolide and lincosamide was detected. A cefoxitin-resistant Staphylococcus saprophyticus was found to be mecA-negative but mecC-positive.


CONCLUSIONS
This study reports for the first time the mecC gene from a CNS in bovine mastitis in South America. Because CNS may act as reservoirs of antimicrobial resistance genes, they can be seen as a potential public health threat with respect to antimicrobial resistance and the development of multiple resistance. Also, the emergence of methicillin-resistant phenotypes will limit therapeutic options.
['Srednik|Mariela E|ME|', 'Archambault|Marie|M|', 'Jacques|Mario|M|', 'Gentilini|Elida R|ER|']
D000818:Animals D000900:Anti-Bacterial Agents D001118:Argentina D001426:Bacterial Proteins D002417:Cattle D002440:Cefoxitin D024881:Drug Resistance, Bacterial D005260:Female D055231:Lincosamides D018942:Macrolides D008414:Mastitis, Bovine D008826:Microbial Sensitivity Tests D017422:Sequence Analysis, DNA D013203:Staphylococcal Infections D057790:Staphylococcus saprophyticus D047090:beta-Lactams
Staphylococcus saprophyticus Bovine mastitis Coagulase-negative staphylococci mecC gene Antimicrobial resistance Methicillin-resistance