A validation study of a multiplex INDEL assay for forensic use in four Chinese populations.
Insertion/deletion (INDELs) marker sets can serve as a useful supplementary tool for human identification. A commercial kit, the Qiagen DIPplex(®) Investigator kit, multiplexes 30 biallelic autosomal INDELs and Amelogenin for forensic use. We performed a validation study based on the DIPplex(®) kit in four Chinese populations: Han, Tibetan, Uyghur, and Kazakh. There were no significant departures from Hardy-Weinberg equilibrium or significant linkage disequilibrium (pair-wised r(2)<0.2) between the 30 INDELs. The random match probabilities were in the range of 3.84 × 10(-11) to 1.20 × 10(-12), and the power of exclusion was >0.99. The multiplex PCR was optimized for a 5-μL volume, full profiles were obtained with 0.062 ng/μL of template DNA, and excellent performance was obtained with degraded casework samples. This study demonstrates that the multiplex INDEL assay can be used as a supplementary method for degraded DNA detection in the studied Chinese populations.
['Wei|Yi-Liang|YL|', 'Qin|Cui-Jiao|CJ|', 'Dong|Hui|H|', 'Jia|Jing|J|', 'Li|Cai-Xia|CX|']
D002681:China D053836:DNA Degradation, Necrotic D016172:DNA Fingerprinting D005006:Ethnicity D005260:Female D005828:Genetics, Population D006801:Humans D054643:INDEL Mutation D008297:Male D018895:Microsatellite Repeats D060885:Multiplex Polymerase Chain Reaction D020641:Polymorphism, Single Nucleotide
INDEL F(st) Heterozygosity Individual identification Population genetics