Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines.
Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to in vitro treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expression and mRNA expression were measured. NO was induced in BMDC from both lines in response to LPS and LPS + heat stimulation; Fayoumi produced more NO with LPS treatment. Fayoumi had higher phagocytic ability and MHC II surface expression. Gene expression for the heat-related genes BAG3, HSP25, HSPA2, and HSPH1 was strongly induced with heat and few differences existed between lines. Expression for the immune-related genes CCL4, CCL5, CD40, GM-CSF, IFN-γ, IL-10, IL-12β, IL-1β, IL-6, IL-8, and iNOS was highly induced in response to LPS and different between lines. This research contributes to the sparse knowledge of genetic differences in chicken BMDC biology and function.
['Van Goor|Angelica|A|', 'Slawinska|Anna|A|', 'Schmidt|Carl J|CJ|', 'Lamont|Susan J|SJ|']
D000818:Animals D017849:Animals, Inbred Strains D030161:Avian Proteins D001854:Bone Marrow Cells D002478:Cells, Cultured D002645:Chickens D003713:Dendritic Cells D004198:Disease Susceptibility D000949:Histocompatibility Antigens Class II D006358:Hot Temperature D007109:Immunity D008070:Lipopolysaccharides D009569:Nitric Oxide D010587:Phagocytosis D013312:Stress, Physiological
Dendritic cells Fayoumi Leghorn Nitric oxide Gene expression MHC-II