Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus.
The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon nuclear translocator (ARNT) genes from the copepod Tigriopus japonicus (Tj) were cloned to examine their potential functions in the invertebrate putative AhR-CYP signaling pathway. The amino acid sequences encoded by the Tj-AhR and Tj-ARNT genes showed high similarity to homologs of Daphnia and Drosophila, ranging from 68% and 70% similarity for the AhR genes to 56% for the ARNT genes. To determine whether Tj-AhR and Tj-ARNT are modulated by environmental pollutants, transcriptional expression of Tj-AhR and Tj-ARNT was analyzed in response to exposure to five concentrations of polychlorinated biphenyl (PCB 126) (control, 10, 50, 100, 500 μg L(-1)), benzo[a]pyrene (B[a]P) (control, 5, 10, 50, 100 μg L(-1)), and tributyltin (TBT) (control, 1, 5, 10, 20 μg L(-1)) 24h after exposure. A time-course experiment (0, 3, 6, 12, 24h) was performed to analyze mRNA expression patterns after exposure to PCB, B[a]P, and TBT. T. japonicus exhibited dose-dependent and time-dependent upregulation of Tj-AhR and Tj-ARNT in response to pollutant exposure, and the degree of expression was dependent on the pollutant, suggesting that pollutants such as PCB, B[a]P, and TBT modulate expression of Tj-AhR and Tj-ARNT genes in the putative AhR-CYP signaling pathway.
['Kim|Bo-Mi|BM|', 'Rhee|Jae-Sung|JS|', 'Hwang|Un-Ki|UK|', 'Seo|Jung Soo|JS|', 'Shin|Kyung-Hoon|KH|', 'Lee|Jae-Seong|JS|']
D000595:Amino Acid Sequence D000818:Animals D051784:Aryl Hydrocarbon Receptor Nuclear Translocator D001564:Benzo(a)pyrene D033342:Copepoda D004305:Dose-Response Relationship, Drug D011078:Polychlorinated Biphenyls D018336:Receptors, Aryl Hydrocarbon D013997:Time Factors D015533:Transcriptional Activation D014220:Trialkyltin Compounds
AhR ARNT Copepod Tigriopus japonicus Polychlorinated biphenyl Benzo[a]pyrene