Control of Protein and Energy Metabolism in the Pituitary Gland in Response to Three-Week Running Training in Adult Male Mice.
It is assumed that crosstalk of central and peripheral tissues plays a role in the adaptive response to physical activity and exercise. Here, we wanted to study the effects of training and genetic predisposition in a marathon mouse model on mRNA expression in the pituitary gland. Therefore, we used a mouse model developed by phenotype selection for superior running performance (DUhTP) and non-inbred control mice (DUC). Both mouse lines underwent treadmill training for three weeks or were kept in a sedentary condition. In all groups, total RNA was isolated from the pituitary gland and sequenced. Molecular pathway analysis was performed by ingenuity pathway analysis (IPA). Training induced differential expression of 637 genes (DEGs) in DUC but only 50 DEGs in DUhTP mice. Genetic selection for enhanced running performance strongly affected gene expression in the pituitary gland and identified 1732 DEGs in sedentary DUC versus DUhTP mice. Training appeared to have an even stronger effect on gene expression in both lines and comparatively revealed 3828 DEGs in the pituitary gland. From the list of DEGs in all experimental groups, candidate genes were extracted by comparison with published genomic regions with significant effects on training responses in mice. Bioinformatic modeling revealed induction and coordinated expression of the pathways for ribosome synthesis and oxidative phosphorylation in DUC mice. By contrast, DUhTP mice were resistant to the positive effects of three-week training on protein and energy metabolism in the pituitary gland.
['Walz|Christina|C|', 'Brenmoehl|Julia|J|0000-0002-7958-0655', 'Trakooljul|Nares|N|0000-0002-3214-2498', 'Noce|Antonia|A|0000-0003-1385-3187', 'Caffier|Caroline|C|', 'Ohde|Daniela|D|0000-0002-0669-8409', 'Langhammer|Martina|M|', 'Wimmers|Klaus|K|0000-0002-9523-6790', 'Ponsuksili|Siriluck|S|0000-0003-4714-1057', 'Hoeflich|Andreas|A|0000-0003-2018-2836']
D000375:Aging D000818:Animals D015536:Down-Regulation D004734:Energy Metabolism D020869:Gene Expression Profiling D008297:Male D051379:Mice D010085:Oxidative Phosphorylation D010641:Phenotype D010805:Physical Conditioning, Animal D010902:Pituitary Gland D011506:Proteins D012333:RNA, Messenger D015203:Reproducibility of Results D012270:Ribosomes D012420:Running D017423:Sequence Analysis, RNA D015854:Up-Regulation
pituitary gland treadmill training pathway analysis DUhTP mice ribosome synthesis oxidative phosphorylation miR-124