The contribution of toll-like receptor 2 on Helicobacter pylori activation of the nuclear factor-kappa B signaling pathway in gastric epithelial cells.
Helicobacter pylori (H. pylori) is a spiral shaped gram-negative bacterium that induces immune responses in the gastric mucosa. Toll-like receptors (TLRs) play important roles in mediating inflammatory cytokines by recognition of conserved molecular patterns on bacteria. Changes in the expression of toll-like receptor (TLR) 2, TLR4 and the relative inflammatory cytokines were analyzed in normal gastric epithelial GES-1 cells following treatment with H. pylori or Escherichia coli lipopolysaccharide (E. coli LPS) in order to investigate the contribution of TLRs in recognizing and mediating the inflammatory response to H. pylori, and study the differences in TLRs' performance between H. pylori and E. coli. Specific inhibitors for the declines in TLR2 and TLR4 were also employed. The results showed that H. pylori infection increased TLR2 expression in GES-1 cells, but TLR4 remained unchanged regardless of H. pylori or TLR2 small interfering RNA treatment. Furthermore, the secretion of cyclooxygenase-2 (COX-2) induced by H. pylori was inhibited by declines in TLR2, but not in TLR4. In conclusion, TLR2 plays an even more important role than TLR4 not only in recognizing H. pylori, but also in the induction of inflammatory cytokines initiated by H. pylori. However, both TLR2 and TLR4 are necessary in mediating the inflammatory response to E. coli LPS.
['Li|Shu|S|', 'Cao|Mei|M|', 'Song|Liju|L|', 'Qi|Panpan|P|', 'Chen|Chong|C|', 'Wang|Xuege|X|', 'Li|Ningzhe|N|', 'Peng|Jingshan|J|', 'Wu|Daoyan|D|', 'Hu|Guoku|G|', 'Zhao|Jian|J|']
D002460:Cell Line D051546:Cyclooxygenase 2 D016207:Cytokines D004847:Epithelial Cells D004926:Escherichia coli D020869:Gene Expression Profiling D016480:Helicobacter pylori D006801:Humans D008070:Lipopolysaccharides D016328:NF-kappa B D015398:Signal Transduction D051195:Toll-Like Receptor 2 D051197:Toll-Like Receptor 4
Toll-like receptor 2 Helicobacter pylori Escherichia coli lipopolysaccharide Cyclooxygenase-2 Toll-like receptor 4