Bioactive apatite incorporated alginate microspheres with sustained drug-delivery for bone regeneration application.
The strontium-substituted hydroxyapatite microspheres (SrHA) incorporated alginate composite microspheres (SrHA/Alginate) were prepared via adding SrHA/alginate suspension dropwise into calcium chloride solution, in which the gel beads were formed by means of crosslinking reaction. The structure, morphology and in vitro bioactivity of the composite microspheres were studied by using XRD, SEM and EDS methods. The biological behaviors were characterized and analyzed through inductively coupled plasma optical emission spectroscopy (ICP-OES), CCK-8, confocal laser microscope and ALP activity evaluations. The experimental results indicated that the synthetic SrHA/Alginate showed similar morphology to the well-known alginate microspheres (Alginate) and both of them possessed a great in vitro bioactivity. Compared with the control Alginate, the SrHA/Alginate enhanced MC3T3-E1 cell proliferation and ALP activity by releasing osteoinductive and osteogenic Sr ions. Furthermore, vancomycin was used as a model drug to investigate the drug release behaviors of the SrHA/Alginate, Alginate and SrHA. The results suggested that the SrHA/Alginate had a highest drug-loading efficiency and best controlled drug release properties. Additionally, the SrHA/Alginate was demonstrated to be pH-sensitive as well. The increase of the pH value in phosphate buffer solution (PBS) accelerated the vancomycin release. Accordingly, the multifunctional SrHA/Alginate can be applied in the field of bioactive drug carriers and bone filling materials.
['Li|Haibin|H|', 'Jiang|Fei|F|', 'Ye|Song|S|', 'Wu|Yingying|Y|', 'Zhu|Kaiping|K|', 'Wang|Deping|D|']
D000464:Alginates D000469:Alkaline Phosphatase D000818:Animals D001672:Biocompatible Materials D001861:Bone Regeneration D002460:Cell Line D049109:Cell Proliferation D004337:Drug Carriers D065546:Drug Liberation D020723:Glucuronic Acid D006603:Hexuronic Acids D006863:Hydrogen-Ion Concentration D006882:Hydroxyapatites D051379:Mice D018613:Microscopy, Confocal D008855:Microscopy, Electron, Scanning D008863:Microspheres D010316:Particle Size D017550:Spectroscopy, Fourier Transform Infrared D013324:Strontium D014640:Vancomycin
Bone regeneration Hydroxyapatite Biological performance Composite alginate microspheres Controlled drug-release