Broad neutralization of CSFV with novel monoclonal antibodies in vivo.
Classical swine fever is a highly contagious disease in China. Although vaccination against Classical swine fever virus (CSFV) has been widely carried out in China, CSFV cases still emerge in an endless stream. Therefore, it is necessary to take new antiviral measures to eliminate CSFV. Glycoprotein E2 of CSFV is the major vaccine candidate that confers protective immunity. Thus, in this study, a batch of neutralizing monoclonal antibodies (mAbs) against E2, as alternative antiviral strategies, were produced. Among them, mAbs 6D10, 8D8 and 3C12 presented neutralizing reactivity against CSFV in a dose-dependent manner. Based on truncated overlapping fragments of E2 and mutants, three linear neutralizing epitopes were identified highly conserved in various CSFV strains. Epitopes 8YRYAIS13 and 254HECLIG259 were reported for the first time. All the three epitopes are involved in virus internalization and attachment as shown in pre- or post-attachment neutralization. Recombinant polypeptides carrying epitopes successfully inhibit virus infection in PK-15 cells, indicating epitopes were located in receptor-binding domain (RBD). Further, both prophylactic and therapeutic functions of neutralizing antibody were evaluated in rabbits upon CSFV challenge, confirming the efficacy in vivo. These findings provide alternative antiviral strategies against CSFV and deepen the understanding in E2 function during virus entry.
['Xu|Huiling|H|', 'Han|Guangwei|G|', 'Lu|Ying|Y|', 'Liu|Zehui|Z|', 'Tao|Lina|L|', 'He|Fang|F|']
D000818:Animals D000911:Antibodies, Monoclonal D057134:Antibodies, Neutralizing D000914:Antibodies, Viral D001665:Binding Sites D002460:Cell Line D002681:China D006691:Classical Swine Fever D006692:Classical Swine Fever Virus D004305:Dose-Response Relationship, Drug D000939:Epitopes D005260:Female D007114:Immunization D051379:Mice D009154:Mutation D000072417:Protein Domains D011817:Rabbits D013552:Swine D014759:Viral Envelope Proteins D014765:Viral Vaccines D053585:Virus Attachment
Neutralization Monoclonal antibody Classical swine fever virus