Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats.
Purpose
Elevation of blood homocysteine (Hcy) level (hyperhomocysteinemia) is a risk factor for cardiovascular disorders and is closely associated with endothelial dysfunction. The present study aims to investigate the protective effect and underlying mechanism of nanoscale selenium (Nano-Se) in Hcy-mediated vascular endothelial cell dysfunction in vitro and in vivo.


Materials and Methods
By incubating vascular endothelial cells with exogenous Hcy and generating hyperhomocysteinemic rat model, the effects of Nano-Se on hyperhomocysteinemia-mediated endothelial dysfunction and its essential mechanisms were investigated.


Results
Nano-Se inhibited Hcy-induced mitochondrial oxidative damage and apoptosis by preventing the downregulation of glutathione peroxidase enzyme 1 and 4 (GPX1, GPX4) in the vascular endothelial cells, thus effectively prevented the vascular damage in vitro and in vivo in the hyperhomocysteinemic rats. Nano-Se possessed similar protective effects but lower toxicity against Hcy in vascular endothelial cells when compared with other forms of Se.


Conclusion
The application of Nano-Se could serve as a novel promising strategy against Hcy-mediated vascular dysfunction with reduced risk of Se toxicity.
['Zheng|Zeqi|Z|', 'Liu|Lijuan|L|', 'Zhou|Kaiwen|K|0000-0001-9804-2783', 'Ding|Lu|L|', 'Zeng|Junyi|J|', 'Zhang|Wan|W|']
D000818:Animals D000975:Antioxidants D017209:Apoptosis D001682:Biological Availability D005979:Glutathione Peroxidase D006710:Homocysteine D061307:Human Umbilical Vein Endothelial Cells D006801:Humans D020138:Hyperhomocysteinemia D006973:Hypertension D008297:Male D008928:Mitochondria D053758:Nanoparticles D010084:Oxidation-Reduction D018384:Oxidative Stress D017207:Rats, Sprague-Dawley D017382:Reactive Oxygen Species D012643:Selenium
nano-selenium homocysteine Nano-Se glutathione peroxidase enzymes GPXs ROS endothelium dysfunction reactive oxygen species