Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. lita.
There is an urgent need for novel strategies to fight drug resistance and multi-drug resistance. As an alternative to the classic antibiotic therapy, attenuation of the bacteria virulence affecting their Quorum sensing (QS) system is a promising approach. Quorum sensing (QS) is a genetic regulation system that allows bacteria to communicate with each other and coordinate group behaviors. A new γ-lactone that is capable of inhibiting the LasI/R QS system, plakofuranolactone (1), was discovered in the extract of the marine sponge Plakortis cf. lita, and its structure, including absolute configuration, was determined by NMR spectroscopy, MS spectrometry, and quantum-mechanical prediction of optical rotation. The quorum quenching activity of plakofuranolactone was evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075, E. coli pSB401, and C. violeaceum CV026) and was confirmed by measuring the total protease activity (a virulence factor which is under control of the LasI/R system) of the wild-type P. aeruginosa PAO1. Further research will be pursued to assess the potential of plakofuranolactone as a new antivirulence lead compound and a chemical tool to increase the knowledge in this field.
['Costantino|Valeria|V|', 'Della Sala|Gerardo|G|', 'Saurav|Kumar|K|', 'Teta|Roberta|R|', 'Bar-Shalom|Rinat|R|', 'Mangoni|Alfonso|A|', 'Steindler|Laura|L|']
D000818:Animals D000900:Anti-Bacterial Agents D000890:Anti-Infective Agents D001419:Bacteria D018432:Drug Resistance, Multiple D004926:Escherichia coli D007214:Indonesia D047229:Plakortis D011161:Porifera D053038:Quorum Sensing D014774:Virulence D037521:Virulence Factors
plakofuranolactone marine sponges quorum quenching activity reporter gene assays antivirulence lead compounds Plakortis lita Pseudomonas aeruginosa antibacterial from marine sponges quorum sensing system