Improvement of Sperm Motility Within One Month Under Selenium and Vitamin E Supplementation in Four Infertile Dogs with Low Selenium Status.
Introduction
Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches.


Material and Methods
The dogs (a Golden Retriever, an English Cocker Spaniel, and two Tibetan Mastiffs) were supplemented daily with selenium (Se) (0.6 mg/kg organic Se yeast) and vitamin E (vit. E) (5 mg/kg) per os for 60 days. Semen was collected on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated by the CASA system, sperm morphology was explored by Diff-Quick staining, and live and dead spermatozoa were differentiated by eosin/nigrosin staining. The concentrations of Se and vit. E were measured in peripheral blood serum on semen collection days.


Results
Before administration, the concentrations of Se in blood plasma were low (86.0-165.0 μg/L). After 30 days of treatment there was an observable improvement in total and progressive sperm motility and kinematic parameters (VAP, VSK, VCL, ALH, BCF, and RAPID). The percentages of live and normal morphology sperm cells were also higher. There was also an observable increase in Se and vitamin E concentrations in blood serum. Bitches were successfully mated and delivered four to six puppies.


Conclusion
Supplementation with Se and vit. E improved rapid sperm motility and restored fertility in infertile dogs with low Se status.
['Domosławska|Anna|A|', 'Zduńczyk|Sławomir|S|', 'Janowski|Tomasz|T|']
selenium vitamin E dog semen fertility