GapmeR-Mediated Gene Silencing in Motile T-Cells.
Gene silencing is an important method to study gene functions in health and diseases. While there are various techniques that are applied to knockdown specific gene(s) of interest, they have certain limitations in application to T-lymphocytes. T-cells are "hard-to-transfect" cells and are recalcitrant to transfection reagents. Here, we describe the use of novel cell-permeating antisense molecules, called "GapmeR", to knockdown specific gene(s) in human primary T-cells.
['Fazil|Mobashar Hussain Urf Turabe|MHUT|', 'Ong|Seow Theng|ST|', 'Chalasani|Madhavi Latha Somaraju|MLS|', 'Kizhakeyil|Atish|A|', 'Verma|Navin Kumar|NK|']
D002465:Cell Movement D002478:Cells, Cultured D055785:Gene Knockdown Techniques D020868:Gene Silencing D005822:Genetic Vectors D006801:Humans D016376:Oligonucleotides, Antisense D013601:T-Lymphocytes D014162:Transfection
T-Lymphocytes GapmeR Gene knockdown RNAi