Evaluation of the hypoglycemic effect of seven wild folkloric edible plants from Palestine.
Alfa-amylase inhibitors are used to reduce glucose absorption by suppressing carbohydrate digestion. The current study aimed to evaluate seven wild edible Palestinian plants' hydrophilic and lipophilic fractions against porcine pancreatic α-amylase enzyme. The lipophilic fractions of Arum palaestinum, Malva sylvestris, Plantago major, Centaurea iberica, Cichorium endivia, Bituminaria bituminosa, Sisymbrium irio leaves were sequentially separated with a nonpolar solvent hexane, while the hydrophilic fractions of the studied plants were separated with polar solvents ethanol and water. The activity of α-amylase inhibition was carried out by using α-amylase porcine pancreatic enzyme and 3,5-dinitrosalicylic acid (DNSA) method as well as by using Acarbose as a positive control. Among the studied plant's hydrophilic fractions, C. iberica and C. endivia have the highest porcine pancreatic α-amylase inhibitory effect with an IC50 value of 12.33 µg/mL and 9.96 µg/mL, respectively. In addition, among the studied plant's lipophilic fractions, S. irio and A. palaestinum have the highest porcine pancreatic α-amylase inhibitory effect with an IC50 value of 7.72 µg/mL and 25.3 µg/mL, respectively. In fact, these revealed results were near the values of Acarbose. The hydrophilic fractions of M. sylvestris and the lipophilic fractions of P. major plants exhibit remarkable α-amylase inhibitory activity. Hence, these leaves have a potential for use as regular supplements also; further investigations are required to isolate pure pharmacological molecules and to design suitable pharmaceutical dosage forms with anti-diabetic activity.
['Hawash|Mohammed|M|https://orcid.org/0000-0001-5640-9700', 'Jaradat|Nidal|N|', 'Elaraj|Josephean|J|', 'Hamdan|Aseel|A|', 'Lebdeh|Saja Abu|SA|', 'Halawa|Tamara|T|']
D000818:Animals D007004:Hypoglycemic Agents D008877:Middle East D010936:Plant Extracts D010945:Plants, Edible D010946:Plants, Medicinal D013552:Swine D000516:alpha-Amylases
Palestine lipophilic fraction α-amylase hydrophilic fraction wild plants