Microwave-assisted solid-phase peptide synthesis of neurosecretory protein GL composed of 80 amino acid residues.
We recently identified a novel cDNA encoding a small secretory protein of 80 amino acid residues, termed neurosecretory protein GL (NPGL), from the chicken hypothalamus. Homologs of NPGL have been reported to be present in mammals, such as human and rat. NPGL is amidated at its C-terminus, contains an intramolecular disulfide bond, and is hydrophobic in nature. In this study, we have optimized the synthesis of the entire 80-amino acid peptide sequence of rat NPGL by microwave-assisted solid-phase peptide synthesis. NPGL was obtained with a 10% yield when the coupling reactions were performed using 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium-3-oxid hexafluorophosphate (HATU) at 50 °C for 5 min, and Fmoc deprotections were performed using 40% piperidine containing 0.1 M HOBt. Furthermore, the disulfide bond of NPGL was formed with 20% yield with the use of glutathione-containing redox buffer and 50% acetonitrile.
['Masuda|Keiko|K|', 'Ooyama|Haruka|H|', 'Shikano|Kenshiro|K|', 'Kondo|Kunihiro|K|', 'Furumitsu|Megumi|M|', 'Iwakoshi-Ukena|Eiko|E|', 'Ukena|Kazuyoshi|K|']
D000596:Amino Acids D000818:Animals D002645:Chickens D006801:Humans D008872:Microwaves D009419:Nerve Tissue Proteins D009474:Neurons D010455:Peptides D051381:Rats D060327:Solid-Phase Synthesis Techniques
solid-phase peptide synthesis NPGL disulfide bond formation microwave small protein