The key long non-coding RNA screening and validation between germinal vesicle and metaphase II of porcine oocyte in vitro maturation.
Oocyte maturation plays a vitally important role in the reproduction of pigs. However, the roles of messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) in the developmental process of porcine oocyte maturation are still largely unclear. In this study, a transcriptome analysis of germinal vesicle (GV) and metaphase II (MII) of oocytes from Chinese Duroc pigs was performed. A total of 1,753,030 and 2,486 differentially expressed (DE) mRNAs, 22,811 and 9,868 DE lncRNAs were identified between GV and MII stages, respectively. Furthermore, functional enrichment analysis showed that the common DE mRNAs and DE lncRNAs during the process of maturation were mainly involved in biological process and cellular components. Our study provides new insights of the expression changes of mRNAs and lncRNAs during GV and MII stages, which might contribute to the maturation of oocytes. These results greatly improve our understanding of the molecular mechanisms regulating the maturation of oocytes in pigs.
['Jiao|Yafei|Y|', 'Gao|Bangjun|B|', 'Wang|Guodong|G|', 'Li|Hui|H|', 'Ahmed|Jam Z|JZ|', 'Zhang|Dandan|D|', 'Ye|Sheng|S|', 'Liu|Shulin|S|', 'Li|Mengmei|M|', 'Shi|Deshun|D|', 'Huang|Ben|B|https://orcid.org/0000-0002-7223-3984']
D000818:Animals D005260:Female D020869:Gene Expression Profiling D018507:Gene Expression Regulation, Developmental D059471:In Vitro Oocyte Maturation Techniques D008677:Metaphase D009865:Oocytes D062085:RNA, Long Noncoding D012333:RNA, Messenger D034421:Sus scrofa
screening oocyte in vitro maturation pig mRNAs IncRNAs RNA-seq