One-pot chemo-enzymatic synthesis of furfuralcohol from xylose.
Furfuralcohol (FOL) is an important intermediate for the production of lysine, ascorbic acid, and lubricants. It can be used as a hypergolic fuel in rocketry. In this study, it was attempted to synthesize FOL from xylose by tandem catalysis with solid acid SO42-/SnO2-Montmorillonite and recombination Escherichia coli CCZU-K14 whole cells. Using SO42-/SnO2-Montmorillonite (3.0wt% dosage) as catalyst, a highest furfural yield of 41.9% was achieved from xylose at 170°C for 20min. Furthermore, Escherichia coli CCZU-K14 whole cells were used for bioconverting furfural to FOL. The optimum biocatalytic reaction temperature, reaction pH, cosubstrate concentration, and substrate concentration were 30°C, 6.5, 1.5mol glucose/mol furfural, and 200mM, respectively. Finally, the yield of FOL from 200mM furfural was achieved to 100% by Escherichia coli CCZU-K14 whole cells after 24h. In conclusion, this strategy show high potential application for the effective synthesis of FOL.
['He|Yu-Cai|YC|', 'Jiang|Chun-Xia|CX|', 'Jiang|Ji-Wei|JW|', 'Di|Jun-Hua|JH|', 'Liu|Feng|F|', 'Ding|Yun|Y|', 'Qing|Qing|Q|', 'Ma|Cui-Luan|CL|']
D000143:Acids D056804:Biofuels D002384:Catalysis D005662:Furaldehyde D013696:Temperature D014994:Xylose
Furfuralcohol Escherichia coli CCZU-K14 Furfural Reductase SO(4)(2−)/SnO(2)-Montmorillonite