Detection of recurrent, rare, and novel gene fusions in patients with acute leukemia using next-generation sequencing approaches.
Identification of gene fusion is an essential part in the management of patients with acute leukemia, not only for diagnosis but also in predicting the treatment outcome and selecting appropriate treatment. Adopting next-generation sequencing (NGS) technology for identification of gene fusion in patients with acute leukemia can be a good alternative to conventional tests. In the present study, the NGS RNA fusion gene panel test was applied to diagnostic samples of patients with acute leukemia to identify fusion genes more efficiently. Among 134 patients with acute leukemia, 53 gene fusions were detected in 52 patients. In addition to the recurrent gene fusions specified in the WHO diagnostic criteria, 11 rare or novel gene fusions were identified. Of those, two were gene fusions associated with Philadelphia-like acute lymphoblastic leukemia (Ph-like ALL), two were novel gene fusions, three were gene fusions with novel partner genes, and six were rare gene fusions from previous reports. We confirmed the clinical utility of the NGS test in identifying clinically significant gene fusions such as gene fusions involving KMT2A that has a large number of partners. Notably, Ph-like ALL-associated gene fusions could be easily identified despite the wide variety of genes involved. The results from the present study may contribute toward a better understanding of the genomic landscape of acute leukemia as well as patient management.
['Kim|Borahm|B|', 'Kim|Esl|E|', 'Lee|Seung-Tae|ST|https://orcid.org/0000-0003-1047-1415', 'Cheong|June-Won|JW|', 'Lyu|Chuhl Joo|CJ|', 'Min|Yoo Hong|YH|', 'Choi|Jong Rak|JR|']
D000293:Adolescent D000328:Adult D000368:Aged D000369:Aged, 80 and over D014408:Biomarkers, Tumor D002648:Child D002675:Child, Preschool D005260:Female D050939:Gene Fusion D023281:Genomics D059014:High-Throughput Nucleotide Sequencing D006801:Humans D015470:Leukemia, Myeloid, Acute D008297:Male D008875:Middle Aged D015514:Oncogene Proteins, Fusion D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma D011379:Prognosis D012008:Recurrence D055815:Young Adult
acute leukemia next-generation sequencing RNA gene fusion detection