SREBP1 suppresses the differentiation and epithelial function of hiPSC-derived endothelial cells by inhibiting the microRNA199b-5p pathway.
Human induced pluripotent stem cell (hiPSC)-derived endothelial cell (hiPSC-EC) transplantation is a promising therapy for treating peripheral artery disease (PAD). However, the poor differentiation of hiPSCs limits their clinical application. Therefore, finding key factors that regulate cellular differentiation is crucial for improving the therapeutic efficacy of hiPSC-EC transplantation. Sterol regulatory element binding protein 1 (SREBP1) is a key regulator of lipid metabolism and stem cell differentiation. However, it remains unknown whether SREPBP1 modulates hiPSC differentiation. In this study, we showed that SREBP1 expression was negatively associated with hiPSC differentiation and EC function. The results show that SREBP1 binds to the promoter region of miR199b-5p and suppresses its transcription, resulting in the activation of Notch1 signaling. Blocking SREBP1 increased both hiPSC differentiation and EC angiogenesis. These findings demonstrate a novel role for SREBP1 in hiPSC differentiation and EC angiogenesis.
['Qian|Xin|X|', 'Guo|Xiangjiang|X|', 'Ni|Qihong|Q|', 'Wang|Han|H|', 'Ye|Meng|M|', 'Zhang|Lan|L|']
D002454:Cell Differentiation D042783:Endothelial Cells D006801:Humans D057026:Induced Pluripotent Stem Cells D015398:Signal Transduction D051780:Sterol Regulatory Element Binding Protein 1
SREBP1 miR199b-5p Human-induced pluripotent stem cells Peripheral vascular diseases