Raman spectrum reveals the cell cycle arrest of Triptolide-induced leukemic T-lymphocytes apoptosis.
Triptolide (TPL), a traditional Chinese medicine extract, possesses anti-inflammatory and anti-tumor properties. Though some research results have implicated that Triptolide (TPL) can be utilized in the treatment of leukemia, it remains controversial about the mechanism of TPL-induced leukemic T-lymphocytes apoptosis. In this study, combining Raman spectroscopic data, principal component analysis (PCA) and atomic force microscopy (AFM) imaging, both the biochemical changes and morphological changes during TPL-induced cell apoptosis were presented. In contrast, the corresponding data during Daunorubicin (DNR)-induced cell apoptosis was also exhibited. The obtained results showed that Raman spectral changes during TPL-induced cell apoptosis were greatly different from DNR-induced cell apoptosis in the early stage of apoptosis but revealed the high similarity in the late stage of apoptosis. Moreover, above Raman spectral changes were respectively consistent with the morphological changes of different stages during TPL-induced apoptosis or DNR-induced apoptosis, including membrane shrinkage and blebbing, chromatin condensation and the formation of apoptotic bodies. Importantly, it was found that Raman spectral changes with TPL-induced apoptosis or DNR-induced apoptosis were respectively related with the cell cycle G1 phase arrest or G1 and S phase arrest.
['Zhang|Daosen|D|', 'Feng|Yanyan|Y|', 'Zhang|Qinnan|Q|', 'Su|Xin|X|', 'Lu|Xiaoxu|X|', 'Liu|Shengde|S|', 'Zhong|Liyun|L|']
D017209:Apoptosis D059447:Cell Cycle Checkpoints D045744:Cell Line, Tumor D048430:Cell Shape D002470:Cell Survival D004224:Diterpenes D004852:Epoxy Compounds D006801:Humans D019169:Jurkat Cells D007938:Leukemia D010616:Phenanthrenes D025341:Principal Component Analysis D013059:Spectrum Analysis, Raman D013601:T-Lymphocytes
Triptolide (TPL) Principal component analysis (PCA) Atomic force microscopy (AFM) Cell apoptosis Daunorubicin (DNR) Raman spectroscopy