Novel Anti-Melanogenesis Properties of Polydeoxyribonucleotide, a Popular Wound Healing Booster.
Polydeoxyribonucleotide (PDRN), a deoxyribonucleotide polymer, is popularly used for faster healing of cutaneous wounds and boosting of neocollagenesis of photoaged skin among current dermatologic practitioners. Some patients receiving PDRN injection treatment also reported improvement of photoaging-associated mottled pigmentation (PMP). To investigate the effect of PDRN on cutaneous melanogenesis, we examined the effect of PDRN and an available product (Placentex(®)) containing PDRN on melanogenesis using human melanocytes-keratinocytes cocultures and mouse melanocytes. Melanin content, tyrosinase activity, and levels of microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein (TRP-1) were determined. Intracellular signaling pathways were assessed by Western blotting. PDRN and Placentex(®) led to decreases in melanin content, tyrosinase activity, and MITF and TRP-1 expression with concomitant increases in phosphorylated forms of extracellular signal-regulated protein kinase (ERK) and AKT in mouse melanocytes. More importantly, both PDRN and Placentex(®) significantly suppressed the melanin content in human melanocyte-keratinocyte cocultures. Clinical evaluation of six female patients with facial hyperpigmentation after three sessions of intradermal PDRN injections using a 5-point scale revealed that PDRN led to more than noticeable improvements in hyperpigmented lesions. This is the first study to demonstrate that PDRN, which is known for its wound-healing properties, may have novel anti-melanogenesis and potential skin whitening properties.
['Noh|Tai Kyung|TK|', 'Chung|Bo Young|BY|', 'Kim|Su Yeon|SY|', 'Lee|Mi Hye|MH|', 'Kim|Moon Jung|MJ|', 'Youn|Choon Shik|CS|', 'Lee|Mi Woo|MW|', 'Chang|Sung Eun|SE|']
D000328:Adult D000368:Aged D000818:Animals D002460:Cell Line D048049:Extracellular Signal-Regulated MAP Kinases D005260:Female D006801:Humans D008543:Melanins D008544:Melanocytes D051379:Mice D051739:Microphthalmia-Associated Transcription Factor D008875:Middle Aged D014442:Monophenol Monooxygenase D011089:Polydeoxyribonucleotides D051057:Proto-Oncogene Proteins c-akt D062327:Skin Lightening Preparations D012880:Skin Pigmentation D014357:Trypsin
polydeoxyribonucleotide melanogenesis coculture hyperpigmentation