Inflammatory and biocompatibility evaluation of antimicrobial peptide GL13K immobilized onto titanium by silanization.
The inflammatory reaction around the implant after implant placement is important not only for osseointegration but also for long-term implant survivals. In our study, GL13K, an antimicrobial peptide, was immobilized onto titanium surfaces to improve its anti-inflammatory properties. The method of silanization was used to immobilize the GL13K, which was confirmed by X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy, water contact angle measurement. DAPI fluorescence staining and Cell Counting Kit-8 (CCK-8) were used to measure the cell attachment and cell viability of the RAW264.7, which indicated a good cytocompatibility. Cellular morphology of RAW264.7 on modified surfaces showed less cell pseudopod. ELISA and qRT-PCR were performed to measure the inflammatory activity of the modified titanium surfaces. The secretion levels of pro-inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α) and inducible nitric oxide synthase (iNOS) were downregulated at 12h, 24h, and 48h, while the anti-inflammatory cytokines IL-10 and arginase were upregulated at 12h, 24h, and 48h. All results indicate that the GL13K-coated titanium surfaces make the inflammatory process towards a less pro-inflammatory, which may promote the process of osseointegration.
['Zhou|Lin|L|', 'Lin|Zhaonan|Z|', 'Ding|Jiamin|J|', 'Huang|Wenxiu|W|', 'Chen|Jiang|J|', 'Wu|Dong|D|']
D000818:Animals D023181:Antimicrobial Cationic Peptides D002470:Cell Survival D016207:Cytokines D055767:Immobilized Proteins D007249:Inflammation D008264:Macrophages D051379:Mice D018625:Microscopy, Atomic Force D008855:Microscopy, Electron, Scanning D009842:Oligopeptides D056951:Photoelectron Spectroscopy D019736:Prostheses and Implants D000067996:RAW 264.7 Cells D012821:Silanes D013499:Surface Properties D014025:Titanium
Inflammatory Antimicrobial peptide Titanium Silanization Macrophage