Analysis of the SNP loci around transcription start sites related to goat fecundity trait base on whole genome resequencing.
Genome flanking regions surrounding transcription start sites (TSSs) are critical for the regulation of gene expression, containing many translational regulatory elements. To investigate whether critical single nucleotide polymorphisms (SNPs) exist around TSSs in the dairy goat genome, we performed high throughput DNA sequencing to compare two dairy goat groups with discrepant litter sizes. After genome mapping, SNP calling, and annotation, we screened the SNPs within 2kb scales surrounding annotated TSSs in high fecundity (HF) and low fecundity (LF) groups, respectively. We attempted to identify distinct SNPs and motifs near the TSSs in both groups. The SNPs near the TSSs most were consistent; 318 new SNPs were uncovered in the HF group, of which 305 were heterozygote SNPs, 13 were homozygote SNPs, and majority of which were distributed on chromosome 2 and 29. After validation by Sanger sequencing we found that a SNP in CHI16: 27612330 C>A in the PSEN2 gene presented an A/A genotype in the HF group and an A/A or A/C genotype in the LF group. In conclusion, our study provides insightful information into the dairy goat genomic variations surrounding TSSs, which may contribute to enhanced litter size. Based on comparison studies of SNPs exist around transcription start sites between high fecundity group and low fecundity group. Our finding provides insights concerning the goat litter size phenotypic and will promote future goat breeding.
['Zhang|Rui-Qian|RQ|', 'Lai|Fang-Nong|FN|', 'Wang|Jun-Jie|JJ|', 'Zhai|Hong-Li|HL|', 'Zhao|Yong|Y|', 'Sun|Yu-Jiang|YJ|', 'Min|Ling-Jiang|LJ|', 'Shen|Wei|W|']
D000818:Animals D001947:Breeding D002874:Chromosome Mapping D005260:Female D005298:Fertility D005838:Genotype D006041:Goats D059014:High-Throughput Nucleotide Sequencing D008098:Litter Size D010641:Phenotype D020641:Polymorphism, Single Nucleotide D011247:Pregnancy D011401:Promoter Regions, Genetic D017422:Sequence Analysis, DNA D024363:Transcription Initiation Site D015533:Transcriptional Activation D000073336:Whole Genome Sequencing
SNPs TSSs Goat genome Whole genome sequencing