Synthesis of γ-N-modified 8-oxo-2'-deoxyguanosine triphosphate and its characterization.
8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes oxodGTP from the nucleotide pools in living cells. On the other hand, the γ-modified triphosphates show interesting properties for use as biological tools. Therefore, the γ-N-pyrenylalkyl-oxodGTP derivatives were synthesized and their effect on the enzymatic reactions were evaluated. The γ-N-pyrenylmethyl-oxodGTP was found to be accepted by the DNA polymerase just like oxodGTP, but showed a competitive inhibition property for the human oxodGTPase.
['Thavoncharoensub|Ninticha|N|', 'Maruyama|Kento|K|', 'Heh|Choon Han|CH|http://orcid.org/0000-0002-3435-1154', 'Hoong Leong|Kok|K|https://orcid.org/0000-0002-7317-6117', 'Shi|Hui|H|', 'Shigematsu|Yoshiharu|Y|', 'Sasaki|Shigeki|S|https://orcid.org/0000-0003-2013-2441', 'Taniguchi|Yosuke|Y|https://orcid.org/0000-0002-8985-3629']
D004259:DNA-Directed DNA Polymerase D003848:Deoxyguanine Nucleotides D006801:Humans D006868:Hydrolysis D007700:Kinetics D062105:Molecular Docking Simulation D015394:Molecular Structure D010084:Oxidation-Reduction D011721:Pyrenes
8-oxodGTP DNA polymerase repair enzyme gamma phosphate modification