Molecular characterization and expression profiles provide new insights into GATA5 functions in tongue sole (Cynoglossus semilaevis).
GATA5 is a member of the GATA transcription factor family, which serves essential roles in varieties of cellular functions and biological processes. In this study, we have accomplished the molecular cloning, bioinformatic analysis and preliminary function study of C. semilaevis GATA5. The full-length cDNA nucleotide sequence is 1955 bp, with a coding sequence of 1167 bp, which encodes a polypeptide of 388 amino acids. Homology, phylogenetic, gene structure and synteny analysis showed that C. semilaevis GATA5 was highly conserved among vertebrates. Tissue distribution pattern exhibited that C. semilaevis GATA5 was significantly expressed in heart, intestine, liver, kidney and gonad, with a sexual dimorphic feature observed in testis and ovary. Embryonic development expression profiles showed that C. semilaevis GATA5 transcripts increased at the blastula stage, and peaked at the heat-beating period. Strong signals were detected at spermatids of male testis and stage III oocytes of female ovary by ISH. The expression of C. semilaevis GATA5 was regulated by 17α-MT and E2 after hormone stimulation to the ovary. Together, all the results pointed out that GATA5 might play a vital role during gonadal maturation and the reproductive cycle of C. semilaevis. This study lays the foundation for further researches on the sex control breeding in tongue sole.
['Liu|Yuxiang|Y|', 'Zhu|He|H|', 'Liu|Yuezhong|Y|', 'Qu|Jiangbo|J|', 'Han|Miao|M|', 'Jin|Chaofan|C|', 'Zhang|Quanqi|Q|', 'Liu|Jinxiang|J|']
D000818:Animals D001947:Breeding D004625:Embryo, Nonmammalian D005260:Female D029941:Fish Proteins D005398:Fisheries D005412:Flatfishes D050992:GATA5 Transcription Factor D020869:Gene Expression Profiling D018507:Gene Expression Regulation, Developmental D008297:Male D010053:Ovary D010802:Phylogeny D012098:Reproduction D026801:Synteny D013737:Testis
Expression profile GATA5 Cynoglossus semilaevis Gonadal development Sex hormones