Phenolic profile and antioxidant activity of Serbian polyfloral honeys.
A total of 58 polyfloral honey samples from different regions in Serbia were studied to determine their phenolic profile, total phenolic content and antioxidant capacity. UHPLC-LTQ OrbiTrap MS made possible the identification of 36 compounds: 24 flavonoids, two abscisic acids, and 10 phenolic acids and their derivatives. Quantification was done using 14 available standards. Data on phenolics and abscisic acids allowed the discrimination and classification of honeys in accordance to their geographical origin, using pattern recognition techniques, principal component analysis and partial least squares discriminant analysis. Samples originated from Vojvodina and Zlatibor region were clearly distinguished from those from the rest of Serbia because of the presence of dicaffeoylquinic acid, ellagic acid, caffeic acid phenethyl ester, and chlorogenic acid, among others. A good correlation (r = 0.865) was observed between total phenolic content and radical-scavenging activity. Total phenolic content ranged from 0.03 to 1.39 mg GAE/g and radical scavenging activity ranged from 1.31% to 25.61%.
['Gašić|Uroš|U|', 'Kečkeš|Silvio|S|', 'Dabić|Dragana|D|', 'Trifković|Jelena|J|', 'Milojković-Opsenica|Dušanka|D|', 'Natić|Maja|M|', 'Tešić|Zivoslav|Z|']
D000040:Abscisic Acid D000975:Antioxidants D002109:Caffeic Acids D002726:Chlorogenic Acid D004610:Ellagic Acid D005419:Flavonoids D005504:Food Analysis D006722:Honey D062385:Hydroxybenzoates D010636:Phenols D010626:Phenylethyl Alcohol D011801:Quinic Acid D055771:Serbia
Phenolic profile Polyfloral honey UHPLC-LTQ OrbiTrap MS Geographical origin Pattern recognition methods