Structural and functional studies of the glycoside hydrolase family 3 β-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii.
The filamentous fungus Hypocrea jecorina produces a number of cellulases and hemicellulases that act in a concerted fashion on biomass and degrade it into monomeric or oligomeric sugars. β-Glucosidases are involved in the last step of the degradation of cellulosic biomass and hydrolyse the β-glycosidic linkage between two adjacent molecules in dimers and oligomers of glucose. In this study, it is shown that substituting the β-glucosidase from H. jecorina (HjCel3A) with the β-glucosidase Cel3A from the thermophilic fungus Rasamsonia emersonii (ReCel3A) in enzyme mixtures results in increased efficiency in the saccharification of lignocellulosic materials. Biochemical characterization of ReCel3A, heterologously produced in H. jecorina, reveals a preference for disaccharide substrates over longer gluco-oligosaccharides. Crystallographic studies of ReCel3A revealed a highly N-glycosylated three-domain dimeric protein, as has been observed previously for glycoside hydrolase family 3 β-glucosidases. The increased thermal stability and saccharification yield and the superior biochemical characteristics of ReCel3A compared with HjCel3A and mixtures containing HjCel3A make ReCel3A an excellent candidate for addition to enzyme mixtures designed to operate at higher temperatures.
['Gudmundsson|Mikael|M|', 'Hansson|Henrik|H|', 'Karkehabadi|Saeid|S|', 'Larsson|Anna|A|', 'Stals|Ingeborg|I|', 'Kim|Steve|S|', 'Sunux|Sergio|S|', 'Fujdala|Meredith|M|', 'Larenas|Edmund|E|', 'Kaper|Thijs|T|', 'Sandgren|Mats|M|']
D018360:Crystallography, X-Ray D032641:Eurotiales D005656:Fungal Proteins D006031:Glycosylation D006868:Hydrolysis D035901:Hypocrea D008031:Lignin D008958:Models, Molecular D011487:Protein Conformation D055503:Protein Multimerization D001617:beta-Glucosidase
glycoside hydrolase β-glucosidase Cel3A thermophilic fungus Rasamsonia emersonii biodegradation crystal structure glycoproteins