The In Vivo Radiosensitizing Effect of Magnolol on Tumor Growth of Hepatocellular Carcinoma.
BACKGROUND/AIM
Radiation (RT) induced ERK/NF-κB in hepatocellular carcinoma (HCC) has been reported in our previous works; it weakens the toxicity of RT or triggers a radioresistance effect. Thus, combining RT with a suitable NF-κB inhibitor may sensitize HCC to RT. Magnolol, a bioactive compound, was known to have anti-inflammatory and anti-tumor functions. Here, we aimed to investigate whether magnolol may enhance anti-HCC efficacy of RT in vivo.


MATERIALS AND METHODS
We established a Hep3B bearing mouse to evaluate the efficacy of the combination treatment of magnolol and RT.


RESULTS
Most significantly, tumor volume and tumor weight inhibition was found in the combination group. Tumor immunohistochemistry staining also illustrated the suppression of RT-induced ERK/NF-κB-related proteins expression by magnolol. In addition, intrinsic apoptosis-related proteins, such as caspase-3 and -9, were markedly increased in the combination group.


CONCLUSION
Magnolol may effectively enhance anti-HCC ability of RT by downregulating the expression of ERK/NF-κB-related proteins and increasing the expression of apoptosis-related proteins.
['Chen|Yu-Shan|YS|', 'Sun|Rou|R|', 'Chen|Wei-Lung|WL|', 'Yau|Yu-Chen|YC|', 'Hsu|Fei-Ting|FT|', 'Chung|Jing-Gung|JG|', 'Tsai|Chia-Jung|CJ|', 'Hsieh|Chia-Ling|CL|', 'Chiu|Ying-Ming|YM|', 'Chen|Jiann-Hwa|JH|']
D000818:Animals D017209:Apoptosis D001713:Biphenyl Compounds D006528:Carcinoma, Hepatocellular D045744:Cell Line, Tumor D017705:Lignans D008113:Liver Neoplasms D051379:Mice D016328:NF-kappa B D011838:Radiation-Sensitizing Agents
Magnolol hepatocellular carcinoma radiation apoptosis