A review of bioinformatic methods for forensic DNA analyses.
Short tandem repeats, single nucleotide polymorphisms, and whole mitochondrial analyses are three classes of markers which will play an important role in the future of forensic DNA typing. The arrival of massively parallel sequencing platforms in forensic science reveals new information such as insights into the complexity and variability of the markers that were previously unseen, along with amounts of data too immense for analyses by manual means. Along with the sequencing chemistries employed, bioinformatic methods are required to process and interpret this new and extensive data. As more is learnt about the use of these new technologies for forensic applications, development and standardization of efficient, favourable tools for each stage of data processing is being carried out, and faster, more accurate methods that improve on the original approaches have been developed. As forensic laboratories search for the optimal pipeline of tools, sequencer manufacturers have incorporated pipelines into sequencer software to make analyses convenient. This review explores the current state of bioinformatic methods and tools used for the analyses of forensic markers sequenced on the massively parallel sequencing (MPS) platforms currently most widely used.
['Liu|Yao-Yuan|YY|', 'Harbison|SallyAnn|S|']
D019295:Computational Biology D016172:DNA Fingerprinting D004272:DNA, Mitochondrial D030541:Databases, Genetic D053803:Forensic Genetics D006239:Haplotypes D059014:High-Throughput Nucleotide Sequencing D006801:Humans D018895:Microsatellite Repeats D020641:Polymorphism, Single Nucleotide
Bioinformatics Massively parallel sequencing Forensic science