Studies on anti-hepatoma activity of Annona squamosa L. pericarp extract.
This study investigated the anti-hepatoma activity of different extracts from A. squamosa pericarps, phytochemistry of the ethyl acetate (EtOAc) fraction and possible anti-hepatoma mechanism of active constituents. The anti-hepatoma activity of different extracts from A. squamosa pericarps were evaluated by MTT assay against SMMC-7721 cells in vitro and verified by using H22 xenografts bearing mice. Phytochemical investigation of the active pericarp extract was carried out. The pro-apoptosis and cycle arrest effects of active constituents were observed by fluorescent microscope and flow cytometry. Western blot assay was conducted to find the possible anti-hepatoma mechanisms of active constituents. The result showed that EtOAc extract was the active fraction. Two ent-kaurane diterpenoids, named ent-kauran-16-en-19-oic acid and ent-kauran-15-en-19-oic acid, were isolated from the active EtOAc fraction. The pro-apoptosis and G1 phase arrest effects of these diterpenoids were found. Western blot assay showed that ent-kauran-16-en-19-oic acid could activate caspase-3,-8,-9, up-regulate of Bax and down-regulate of Bcl-2.
['Chen|Ya-Yun|YY|', 'Cao|Yu-Zhu|YZ|', 'Li|Fu-Qiang|FQ|', 'Zhu|Xiao-Li|XL|', 'Peng|Chen-Xiao|CX|', 'Lu|Jia-Hui|JH|', 'Chen|Jian-Wei|JW|', 'Li|Xiang|X|', 'Chen|Yong|Y|']
D000818:Animals D031041:Annona D000972:Antineoplastic Agents, Phytogenic D017209:Apoptosis D006528:Carcinoma, Hepatocellular D045744:Cell Line, Tumor D045786:Diterpenes, Kaurane D006801:Humans D008099:Liver D008113:Liver Neoplasms D051379:Mice
Anti-hepatoma Annona squamosa Mechanism Diterpenoid