Tylophorine Abrogates G2/M Arrest Induced by Doxorubicine and Promotes Increased Apoptosis in T47D Breast Cancer Cells
Background: The effects of tylophorine, a natural alkaloid found in Tylophora indica, administered as a single
compound or in combination with doxorubicin on cell cycling and apoptosis were assessed in T47D breast cancer cells,
selected as a model system for breast cancer. Methods: Cell cycle distribution and apoptosis were examined by flow
cytometry. Caspase 3 and 9 expression was determined by immunocytochemistry.Result: We found that tylophorine did
not significantly influence the cell cycle distribution of T47D cells. However, the alkaloid did prevent accumulation of
cells in the G2/M phase. In addition, tylophorine increased the number of apoptotic cells. Expression of proapoptotic
proteins (caspases 3 and 9) was up-regulated upon administration of tyloporine alone or in combination with doxorubicin.
Conclusions: Tylophorine alone or in combination with doxorubicin induced apoptosis in T47D breast cancer cells
through modulation of the cell cycle and affecting the expression of caspases 3 and 9.
['Pratama|Nofran Putra|NP|', 'Wulandari|Septi|S|', 'Nugroho|Agung Endro|AE|', 'Fakhrudin|Nanang|N|', 'Astuti|Puji|P|', 'Sudarsono|||']
D000470:Alkaloids D000903:Antibiotics, Antineoplastic D017209:Apoptosis D001943:Breast Neoplasms D002453:Cell Cycle D049109:Cell Proliferation D004317:Doxorubicin D004359:Drug Therapy, Combination D005260:Female D006801:Humans D007212:Indolizines D010616:Phenanthrenes D014407:Tumor Cells, Cultured
Tylophorine apoptosis T47D cell cycle caspase 3 and 9