ARCHITECT® urine-neutrophil gelatinase-associated lipocalin (u-NGAL) assay as new prognostic marker for clear cell Renal Cell Carcinoma (ccRCC) (preliminary results).
INTRODUCTION
Biomarkers for the diagnosis and monitoring treatment response of kidney cancer are urgently needed. Neutrophil gelatinase-associated lipocalin (NGAL) is a relevant urinary biomarker for the diagnosis of a wide variety of acute and chronic kidney diseases. Its potential utility as a prognostic marker of kidney cancer is largely unknown and, therefore, was the subject of this investigation.


MATERIALS AND METHODS
A retrospective study was done on 50 kidney tumor patients (urine samples prospectively collected before nephrectomy between 2004 and 2012, stored at Biobank Resource Center). The specificity, sensitivity and the predictive value of NGAL were determined for progression-free and disease-specific survival after nephrectomy in renal cell carcinoma (particularly, the clear cell renal cell carcinoma (ccRCC)). Urinary NGAL concentration (u-NGAL) was determined by CMIA technique (ARCHITECT® urine NGAL essay/ABBOTT®).


RESULTS
Out of the 50 kidney tumor patients, 40 had clear cell carcinoma with a median u-NGAL excretion of 1.4 (IQR: 5.76) ng/mg urinary creatinine (Ucr). u-NGAL was correlated to tumor stage (p = 0.005), and Fuhrman grade (p = 0.0002). Multivariate Cox regression analysis showed a significant association between u-NGAL excretion and clear cell renal cell carcinoma progression free survival and disease specific survival (p = 0.002; p = 0.0001).


CONCLUSIONS
Urinary NGAL was significantly associated with the stage and the grade of kidney cancer. u-NGAL excretion could be considered as a potential biomarker to identify ccRCC patients with the more pejorative outcomes.
['Ben Khadhra|Hajer|H|', 'Rose-Robert|Françoise|F|', 'Herpe|Yves Edouard|YE|', 'Sevestre|Henri|H|', 'Choukroun|Gabriel|G|', 'Catherine|Luc|L|', 'Amant|Carole|C|', 'Saint|Fabien|F|']
D000328:Adult D000368:Aged D014408:Biomarkers, Tumor D002292:Carcinoma, Renal Cell D005260:Female D006801:Humans D007680:Kidney Neoplasms D000071068:Lipocalin-2 D008297:Male D008875:Middle Aged D011379:Prognosis D012189:Retrospective Studies
NGAL Clear cell renal carcinoma Urinary markers