Genome-wide expression analysis reveal host genes involved in immediate-early infections of different sheeppox virus strains.
This study explored the transcriptome of lamb testis cells infected with sheeppox virus (SPPV) wild strain (WS) and vaccine strain (VS) at an immediate-early time. Most of the differentially expressed genes (DEGs) and differentially expressed highly connected (DEHC) gene network were found to be involved in SPPV-VS infection compared to SPPV-WS. Further, the signaling pathways were mostly involved in SPPV-VS infection than SPPV-WS. SPPV modulates the expression of several important host proteins such as CD40, FAS, ITGβ1, ITGα1, Pak1, Pak2, CD14, ILK leading to viral attachment and entry; immune-related DEGs such as MAPK, JNK, ERK, NFKB, IKB, PI3K, STAT which provide optimal cellular condition for early viral protein expression; and FOXO3, ATF, CDKNA1, TCF, SRF, BDNF which help in inducing apoptosis and MPTP, BAD and Tp53 inhibits apoptosis or cell death at the immediate-early time. The results captured the specific genes and enabled to understand distinct pathogenic mechanisms employed by VS and WS of SPPV.
['Sonowal|Joyshikh|J|', 'Lal Patel|Chhabi|C|', 'Kumar Gandham|Ravi|R|', 'Sajjanar|Basavaraj|B|', 'Ishaq Nabi Khan|Raja|R|', 'Ranjan Praharaj|Manas|M|', 'Akram Malla|Waseem|W|', 'Kumar|Deepak|D|', 'Dev|Kapil|K|', 'Barkathullah|N|N|', 'Bharali|Krishna|K|', 'Dubey|Amitesh|A|', 'Lalita|D|D|', 'Zafir|Insha|I|', 'Mishra|B P|BP|', 'Mishra|Bina|B|']
D000818:Animals D018151:Capripoxvirus D002478:Cells, Cultured D015870:Gene Expression D017781:Genes, Immediate-Early D054884:Host-Pathogen Interactions D008297:Male D011213:Poxviridae Infections D060066:Protein Interaction Maps D012756:Sheep D012757:Sheep Diseases
Sheeppox virus Transcriptome Lamb testis cells Immediate early infection RNA-Seq analysis SPPV vaccine strain SPPV wild strain