Protective effects of skin permeable epidermal and fibroblast growth factor against ultraviolet-induced skin damage and human skin wrinkles.
BACKGROUND
Epidermal and fibroblast growth factor (EGF and FGF1) proteins play an important role in the regeneration and proliferation of skin cells. EGF and FGF1 have considerable potential as possible therapeutic or cosmetic agents for the treatment of skin damage including wrinkles.


OBJECTIVES
Using protein transduction domains (PTD), we investigated whether PTD-EGF and FGF1 transduced into skin cells and tissue. Transduced proteins showed protective effects in a UV-induced skin damage model as well as against skin wrinkles.


METHODS
Transduced PTD-EGF and FGF1 proteins were detected by immunofluorescence and immunohistochemistry. The effects of PTD-EGF and FGF1 were examined by WST assay, Western blotting, immunohistochemistry, and skin wrinkle parameters.


RESULTS
The PTD-EGF and FGF1 increased cell proliferation and collagen type 1 alpha 1 protein accumulation in skin tissue. Also, PTD-EGF and FGF1 inhibited UV-induced skin damage. Furthermore, topical application of PTD-EGF and FGF1 contained ampoules which were considered to improve the wrinkle parameters of human skin.


CONCLUSION
These results show that PTD-EGF and FGF1 can be a potential therapeutic or cosmetic agent for skin damaged and injury including wrinkles and aging.
['An|Jae Jin|JJ|', 'Eum|Won Sik|WS|', 'Kwon|Hyuck Se|HS|', 'Koh|Jae Sook|JS|', 'Lee|Soo Yun|SY|', 'Baek|Ji Hwoon|JH|', 'Cho|Yong-Jun|YJ|', 'Kim|Dae Won|DW|', 'Han|Kyu Huyng|KH|', 'Park|Jinseu|J|', 'Jang|Sang Ho|SH|', 'Choi|Soo Young|SY|']
D000818:Animals D003879:Dermatologic Agents D004195:Disease Models, Animal D004815:Epidermal Growth Factor D016220:Fibroblast Growth Factor 1 D006801:Humans D051379:Mice D008810:Mice, Inbred C57BL D012867:Skin D015595:Skin Aging D016896:Treatment Outcome D014466:Ultraviolet Rays
wrinkle PTD-EGF and FGF1 UV protein therapy skin permeation