Identification of a four-microRNA panel in serum as promising biomarker for colorectal carcinoma detection.
Background: Screening for colorectal carcinoma (CRC) lacks an efficient, inexpensive and noninvasive approach. The stable presence of serum miRNA is expected to become a new diagnostic marker. Materials & methods: Based on 135 CRC patients and 135 normal controls, this study was conducted in three phases to identify suitable serum miRNA for CRC diagnosis by using quantitative reverse transcription PCR. Bioinformatic assays were used for target genes prediction and functional annotation. Results: Serum expression level of seven miRNAs were significantly different between CRC patients and the normal controls. The final diagnostic panel (area under the curve = 0.893; sensitivity = 81.25%, specificity = 73.33%) consists of miR-203a-3p, miR-145-5p, miR-375-3p and miR-200c-3p. Conclusion: The four-miRNA panel may serve as a novel, noninvasive biomarker for CRC diagnosis and screening.
['Huang|Guocheng|G|', 'Wei|Benlin|B|', 'Chen|Zebo|Z|', 'Wang|Jingyao|J|', 'Zhao|Liwen|L|', 'Peng|Xiqi|X|', 'Liu|Kaihao|K|', 'Lai|Yongqing|Y|', 'Ni|Liangchao|L|']
D000328:Adult D014408:Biomarkers, Tumor D016022:Case-Control Studies D015179:Colorectal Neoplasms D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008297:Male D035683:MicroRNAs D008875:Middle Aged D012372:ROC Curve
biomarker miR-203a-3p miR-145-5p miR-375-3p miR-200c-3p colorectal cancer microRNA