Oral fluid versus blood sampling in group-housed sows and finishing pigs: Feasibility and performance of antibody detection for porcine reproductive and respiratory syndrome virus (PRRSV).
The feasibility of using individual and pen-based oral fluid samples to detect PRRSV antibodies in growing-finishing pigs and group-housed sows was investigated. The diagnostic performances of a commercial oral fluid ELISA (OF-ELISA) and a serum ELISA (SER-ELISA) performed on individual or pooled samples from 5 or 10 pigs and sows was evaluated. The performance of the OF-ELISA was also assessed for pen-based oral fluids. Eight hundred and thirty-four pigs and 1598 sows from 42 PRRSV-infected and 3 PRRSV-negative herds were oral fluid sampled and bled. PRRSV antibodies were detected by an OF-ELISA performed at individual, pool (5 or 10 samples) and pen levels. Serum samples were tested by a SER-ELISA at individual and pool levels. The sensitivity and specificity of ELISAs for individual samples were assessed by Bayesian analysis. The relative diagnostic performance for the pools was calculated by taking individual samples as the gold standard. SER-ELISA and individual OF-ELISA results were used as references for estimating OF-ELISA performance for pen-based samples. Individual oral fluid collection was feasible in all kinds of pigs, whereas pen-based samples were unsuccessful in 40% of the group-housed sow pens. High levels of sensitivity comparable to those of the SER-ELISA were found for the OF-ELISA when performed on individual, 5-sample pool or pen-based samples from pigs or sows. The OF-ELISA lacked specificity for individual samples from sows. Pooling 5 individual oral fluid samples or using pen-based samples increased test specificity.
['Fablet|C|C|', 'Renson|P|P|', 'Pol|F|F|', 'Dorenlor|V|V|', 'Mahé|S|S|', 'Eono|F|F|', 'Eveno|E|E|', 'Le Dimna|M|M|', 'Liegard-Vanhecke|D|D|', 'Eudier|S|S|', 'Rose|N|N|', 'Bourry|O|O|']
D000818:Animals D000914:Antibodies, Viral D004797:Enzyme-Linked Immunosorbent Assay D005260:Female D019318:Porcine Reproductive and Respiratory Syndrome D019316:Porcine respiratory and reproductive syndrome virus D013048:Specimen Handling D013552:Swine
Oral fluid PRRSV antibody Diagnostic performance ELISA Serum