Transferable Multidrug-Resistance Plasmid Carrying a Novel Macrolide-Clindamycin Resistance Gene, erm(50), in Cutibacterium acnes.
Antimicrobial-resistant Cutibacterium acnes strains have emerged and disseminated throughout the world. The 23S rRNA mutation and erm(X) gene are known as the major resistance determinants of macrolides and clindamycin in C. acnes We isolated eight high-level macrolide-clindamycin-resistant C. acnes strains with no known resistance determinants, such as 23S rRNA mutation and erm(X), from different acne patients in 2008 between 2013 and 2015. The aim of this study was to identify the novel mechanisms of resistance in C. acnes Whole-genome sequencing revealed the existence of a plasmid DNA, denoted pTZC1 (length, 31,440 bp), carrying the novel macrolide-clindamycin resistance gene erm(50) and tetracycline resistance gene tet(W). pTZC1 was detected in all C. acnes isolates (eight strains) exhibiting high-level macrolide-clindamycin resistance, with no known resistance determinants (MIC of clarithromycin, ≥256 μg/ml; clindamycin, ≥256 μg/ml). Transconjugation experiments demonstrated that the pTZC1 was horizontally transferred among C. acnes strains and conferred resistance to macrolides, clindamycin, and tetracyclines. Our data showed, for the first time, the existence of a transferable multidrug-resistant plasmid in C. acnes Increased prevalence of this plasmid will be a great threat to antimicrobial therapy for acne vulgaris.
['Aoki|Sae|S|', 'Nakase|Keisuke|K|0000-0002-9205-3008', 'Nakaminami|Hidemasa|H|0000-0003-2889-8257', 'Wajima|Takeaki|T|0000-0001-9506-707X', 'Hayashi|Nobukazu|N|', 'Noguchi|Norihisa|N|']
D000152:Acne Vulgaris D000900:Anti-Bacterial Agents D001426:Bacterial Proteins D002981:Clindamycin D003227:Conjugation, Genetic D024901:Drug Resistance, Multiple, Bacterial D015870:Gene Expression D022761:Gene Transfer, Horizontal D016680:Genome, Bacterial D006801:Humans D018942:Macrolides D008826:Microbial Sensitivity Tests D010802:Phylogeny D010957:Plasmids D011423:Propionibacteriaceae D012338:RNA, Ribosomal, 23S D013753:Tetracycline Resistance D013754:Tetracyclines D000073336:Whole Genome Sequencing
plasmid erm(50) Cutibacterium acnes tet(W) multidrug resistance