Serum metabolites may be useful markers to assess vascular invasion and identify normal alpha-fetoprotein in hepatocellular carcinoma undergoing liver resection: a pilot study.
PURPOSE
Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver with a dismal prognosis. Vascular invasion, among others, is the most robust indicator of postoperative recurrence and overall survival after liver resection for HCC. Few studies to date have attempted to search for effective markers to predict vascular invasion before the operation. The current study would examine the plasma metabolic profiling via 1H-NMR of HCC patients undergoing liver resection and aim to search for potential biomarkers in the early detection of HCC with normal alpha-fetoprotein (AFP) and the diagnosis of vascular invasion preoperatively.


MATERIALS AND METHODS
HCC patients scheduled to receive liver resections for their HCC were recruited and divided into two separate groups, investigation cohort and validation cohort. Their preoperative blood samples were collected and subjected to a comprehensive metabolomic profiling using 1H-nuclear magnetic resonance spectroscopy (NMR).


RESULTS
There were 35 HCC patients in the investigation group and 22 patients in the validation group. Chronic hepatitis B remained the most common etiology of HCC, followed by chronic HCV infection. The two study cohorts were essentially comparable in terms of major clinicopathological variables. After 1H-nuclear NMR analysis, we found in the investigation cohort that HCC with normal alpha-fetoprotein (AFP < 15 ng/mL) had significantly higher serum level of O-acetylcarnitine than those with higher AFP (AFP ≥ 15 ng/mL, P = 0.025). In addition, HCC with microscopic vascular invasion (VI) had significantly higher preoperative serum level of formate than HCC without microscopic VI (P = 0.023). These findings were similar in the validation cohort.


CONCLUSION
A comprehensive metabolomic profiling of HCC demonstrated that serum metabolites may be utilized to assist the early diagnosis of AFP-negative HCC patients and recognition of microvascular invasion in order to facilitate preoperative surgical planning and postoperative follow-up. Further, larger scale prospective studies are warranted to consolidate our findings.
['Lee|Chao-Wei|CW|http://orcid.org/0000-0002-6853-4557', 'Yu|Ming-Chin|MC|', 'Lin|Gigin|G|', 'Chiu|Jo-Chu|JC|', 'Chiang|Meng-Han|MH|', 'Sung|Chang-Mu|CM|', 'Hsieh|Yi-Chung|YC|', 'Kuo|Tony|T|', 'Lin|Cheng-Yu|CY|', 'Tsai|Hsin-I|HI|']
D000368:Aged D014408:Biomarkers, Tumor D006528:Carcinoma, Hepatocellular D005260:Female D006498:Hepatectomy D006801:Humans D008113:Liver Neoplasms D008297:Male D009361:Neoplasm Invasiveness D009364:Neoplasm Recurrence, Local D010865:Pilot Projects D011379:Prognosis D011446:Prospective Studies D012372:ROC Curve D012307:Risk Factors D000509:alpha-Fetoproteins
Metabolites Vascular invasion Hepatocellular carcinoma Metabolomics Formate Hepatoma