Preparation of temperature sensitive molecularly imprinted polymer for solid-phase microextraction coatings on stainless steel fiber to measure ofloxacin.
A kind of new temperature sensitive molecularly imprinted polymer (MIP) with ofloxacin (OFL) as template was prepared for the coating of solid phase microextraction (SPME). Dopamine was self-polymerized on stainless steel fiber (SSF) as the SPME support followed by silanization. Then MIP was synthesized as SPME coating on the modified SSF in a capillary, with N-isopropyl acrylamide as temperature sensitive monomer and methacrylic acid as functional monomer. The synthesis could be well repeated with multiple capillaries putting in the same reaction solution. The obtained MIP fiber was evaluated in detail with different techniques and various adsorption experiments. At last the MIP fiber was used to extract the OFL in milk. Satisfied recoveries between 89.7 and 103.4% were obtained with the limit of quantification (LOQLC) of 0.04 μg mL(-1) by the method of SPME coupled with high performance of liquid chromatography (HPLC).
['Zhao|Tong|T|', 'Guan|Xiujuan|X|', 'Tang|Wanjin|W|', 'Ma|Ying|Y|', 'Zhang|Haixia|H|']
D000327:Adsorption D000818:Animals D002851:Chromatography, High Pressure Liquid D008892:Milk D054802:Molecular Imprinting D015242:Ofloxacin D011108:Polymers D052617:Solid Phase Microextraction D013193:Stainless Steel D013696:Temperature
Temperature sensitive Molecularly imprinted polymer Ofloxacin Solid phase microextraction