Bisphenol A decreases expression of Insulin-like factor 3 and induces histopathological changes in the Testes of Rats.
Bisphenol A (BPA) is a chemical agent known to have detrimental reproductive and developmental effects. The tissue-specific impacts of BPA exposures and target tissues sensitiveness to BPA are still unclear. The aim of this study was to determine the short- and long-term dose-dependent toxic effects of BPA on rat testes. Forty-eight Wistar albino male rats were divided into four groups each containing 12 rats. To induce toxicity, BPA was administered orally at three different dosages (50, 100, and 200 mg/kg) for 14 and 28 days, respectively. Testis tissues were examined using light and electron microscopy, immunohistochemistry, and biochemical methods. Serum testosterone (T) and luteinizing hormone (LH) levels were measured. Additionally, insulin-like factor 3 (INSL3) as a marker of Leydig cell function was evaluated immunohistochemically. Groups administered high doses of BPA showed severe degenerations such as testicular atrophy, spermatogenic arrest, and interstitial edema in testis. Also, a significant decrease in INSL3 immunoreactivity and serum LH and T levels was found. The results indicated that both increased exposure time and dosage of BPA caused more serious detrimental effects on testes in the rat. Decreased INSL3 and T levels was evidence of Leydig cell function impairment due to BPA.
['Sencar|Leman|L|https://orcid.org/0000-0002-6301-0308', 'Coskun|Gulfidan|G|', 'Şaker|Dilek|D|', 'Sapmaz|Tuğçe|T|', 'Tuli|Abdullah|A|', 'Özgür|Hülya|H|', 'Polat|Sait|S|']
D000328:Adult D000818:Animals D001559:Benzhydryl Compounds D004305:Dose-Response Relationship, Drug D005260:Female D006801:Humans D008297:Male D023421:Models, Animal D010636:Phenols D011247:Pregnancy D051381:Rats D017208:Rats, Wistar D013002:Somatomedins D013737:Testis D013739:Testosterone
bisphenol A Testis microscopy testosterone INSL3