Retrospective analysis on the consistency of MRI features with histological and molecular markers in diffuse intrinsic pontine glioma (DIPG).
PURPOSE
The diagnosis of diffuse intrinsic pontine glioma (DIPG) is based largely on a combination of clinical and radiological findings due to the difficulty of obtaining a biopsy. An accurate evaluation of magnetic resonance imaging (MRI) scans is consequently essential. Recent analyses on the genomic landscape of DIPG revealed recurrent mutations in the H3F3A and HIST1H3B histone genes. We reviewed cases with available tumor tissue from institutional DIPG series to ascertain the consistency between their histo-molecular findings and clinical-radiological features.


METHODS
We conducted a radiological and pathological central review of 22 cases enrolled in institutional DIPG trials. We performed immunohistochemical analyses to detect H3F3A/HIST1H3B K27M mutations, histone trimethylation, and EZH2 expression. Mutational analysis was performed for ACVR1, H3F3A, and HIST1H3B genes.


RESULTS
Patients' median age at diagnosis was 8 years, and their median overall survival was 11 months. Nineteen/22 cases (86%) showed evidence of K27M mutation on immunohistochemistry and/or mutation analysis. Histone trimethylation expression was low or lacking in these mutated cases. Sequence analysis revealed 13 cases with H3F3A and 1 case with HIST1H3B K27M mutation. There was no significant difference in EZH2 expression between the K27M mutant and wild-type DIPGs. Upon external, blinded MRI re-evaluation one lesion not consistent with DIPG showed no evidence of K27M mutation and retained histone trimethylation expression.


CONCLUSION
In conclusion, our study demonstrates a high frequency of histone K27M mutations in DIPG when MRI features are carefully assessed, thus confirming the consistency of imaging with biological markers in our institutional series of DIPG.
['Giagnacovo|Marzia|M|', 'Antonelli|Manila|M|', 'Biassoni|Veronica|V|', 'Schiavello|Elisabetta|E|', 'Warmuth-Metz|Monika|M|', 'Buttarelli|Francesca R|FR|', 'Modena|Piergiorgio|P|', 'Massimino|Maura|M|']
D015415:Biomarkers D020295:Brain Stem Neoplasms D000080443:Diffuse Intrinsic Pontine Glioma D005971:Glutamates D006801:Humans D008279:Magnetic Resonance Imaging D009154:Mutation D012189:Retrospective Studies
Glioma DIPG H3F3A HIST1H3B