Variation in the Toll-like Receptor 4 (TLR4) gene affects milk traits in dairy cows.
The objective of this Research Communication was to use polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis to investigate a region of the bovine TLR4 gene (TLR4) in pasture-fed New Zealand (NZ) Holstein-Friesian × Jersey (HF × J) cross dairy cows and to determine whether gene variation was associated with milk production traits. Genetic variation was observed, with two variants (A and B) containing a single nucleotide polymorphism (SNP) (c.2021C/T) that was non-synonymous and putatively results in a p.Thr674Ile substitution in the transmembrane/cytoplasmic domain of TLR4. Variant A was associated with higher milk yields, but lower milk fat percentages, whereas B was associated with lower milk yields, but higher fat and protein percentages. Cows of genotype AA produced more milk than AB or BB cows, but the milk produced by AA cows contained less fat than AB or BB cows.
['Zhou|Huitong|H|', 'Cheng|Long|L|', 'Gong|Hua|H|', 'Byun|Seung O|SO|', 'Edwards|Grant R|GR|', 'Hickford|Jon G H|JGH|']
D000818:Animals D002417:Cattle D003612:Dairying D005223:Fats D005260:Female D014644:Genetic Variation D005838:Genotype D007774:Lactation D008892:Milk D008894:Milk Proteins D009520:New Zealand D016133:Polymerase Chain Reaction D020641:Polymorphism, Single Nucleotide D018807:Polymorphism, Single-Stranded Conformational D051197:Toll-Like Receptor 4
milk production traits single nucleotide polymorphism (SNP) Toll-like receptor 4 gene (TLR4)