New Cyclohexadepsipeptides from an Entomogenous Fungus Fusarium proliferatum and Their Cytotoxicity and Autophagy-Inducing Activity.
Five new cyclohexadepsipeptides termed as enniatins R - V (1 - 5) and seven known cyclohexadepsipeptides (6 - 12) were isolated from the solid culture of Fusarium proliferatum, a fungus isolated from the cadaver of an unidentified insect collected in Tibet. Their structures were elucidated by NMR and MS spectroscopic analysis. The X-ray single-crystal structure of 6 was reported for the first time. Enniatins R and S represented the first enniatins incorporating with an unusual 2,3-dihydroxy-isovaleric acid (Div) residue. The cytotoxicity and autophagy-inducing activities of 1 - 12 were evaluated in vitro. Beauvenniatin F (11) exhibited strong cytotoxicity against K562/A (adriamycin-resistant K562) with IC50 value of 3.78 μm, and also autophagy-inducing activity at the concentration of 20 μm in GFP-LC3 stable HeLa cells.
['Tian|Jin|J|', 'Han|Jun-Jie|JJ|', 'Zhang|Xue|X|', 'He|Lu-Wei|LW|', 'Zhang|Yong-Jie|YJ|', 'Bao|Li|L|', 'Liu|Hong-Wei|HW|']
D000970:Antineoplastic Agents D001343:Autophagy D045744:Cell Line, Tumor D049109:Cell Proliferation D002470:Cell Survival D018360:Crystallography, X-Ray D047630:Depsipeptides D004305:Dose-Response Relationship, Drug D005670:Fusarium D006801:Humans D008958:Models, Molecular D008968:Molecular Conformation D013329:Structure-Activity Relationship
Cyclohexadepsipeptides Fusarium proliferatum Cytotoxicity Autophagy-inducing activity