Gangliosides profiling in serum of breast cancer patient: GM3 as a potential diagnostic biomarker.
Gangliosides altered during the pathological conditions and particularly in cancers. Here, we aimed to profile the gangliosides in breast cancer serum and propose potential biomarkers. LC-FTMS method was first used to identify all the ganglioside species in serum, then LC-MS/MS-MRM method was employed to quantitate the levels of gangliosides in serum from healthy volunteers and patients with benign breast tumor or breast cancer. 49 ganglioside species were determined, including GM1, GM2, GM3, GD1, GD3 and GT1 species. Compared to healthy volunteers, the levels of GM1, GM2, GM3, GD1 and GD3 displayed a rising trend in breast cancer patients. In particular, as the major glycosphingolipid component, GM3 showed excellent diagnostic accuracy in cancer serum (AUC > 0.9). PCA profile of the GM3 species showed clear distinction between normal and cancer serum. What's more, ROC curve proved great diagnostic accuracy of GM3 between cancer and benign serum. In addition, GM3 was discovered as a diagnostic marker to differentiate luminal B subtype from other subtypes. Furthermore, a positive correlation between GM3 and Ki-67 status of patients was identified. In conclusion, our results introduced the alteration patterns of serum gangliosides in breast cancer and suggested serum GM3 as a potential diagnostic biomarker in breast cancer diagnosis and luminal B subtype distinction.
['Li|Qinying|Q|', 'Sun|Mei|M|', 'Yu|Mingsheng|M|', 'Fu|Qianyun|Q|', 'Jiang|Hao|H|', 'Yu|Guangli|G|', 'Li|Guoyun|G|']
D000328:Adult D000368:Aged D019540:Area Under Curve D014408:Biomarkers, Tumor D001943:Breast Neoplasms D016022:Case-Control Studies D002853:Chromatography, Liquid D003937:Diagnosis, Differential D005260:Female D005679:G(M3) Ganglioside D005732:Gangliosides D006801:Humans D019394:Ki-67 Antigen D008875:Middle Aged D009369:Neoplasms D025341:Principal Component Analysis D011379:Prognosis D012372:ROC Curve D053719:Tandem Mass Spectrometry
Gangliosides Serum Breast cancer GM3 Biomarker