Permethrin Resistance Status and Associated Mechanisms in Aedes albopictus (Diptera: Culicidae) From Chiapas, Mexico.
There are major public health concerns regarding the spread of mosquito-borne diseases such as dengue, Zika, and chikungunya, which are mainly controlled by using insecticides against the vectors, Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse). Pyrethroids are the primary class of insecticides used for vector control, due to their rapid knockdown effect and low toxicity to vertebrates. Unfortunately, continued use of pyrethroids has led to widespread insecticide resistance in Ae. aegypti; however, we lack information for Ae. albopictus-a sympatric species in Chiapas since 2002. In this study, we evaluated the permethrin resistance status of Ae. albopictus collected from Mexico and Texas. We also selected for permethrin resistance in the laboratory and investigated the potential mechanisms conferring resistance in this species. Knockdown resistance mutations, specifically F1534C, in the voltage-gated sodium channel gene, and increased activity of detoxifying enzymes were evaluated. Low levels of permethrin resistance (<2.4-fold) were observed in our field populations of Ae. albopictus and the F1534C mutation was not detected in any of the sites. Low levels of resistance were also observed in the artificially selected strain. There was significantly higher cytochrome P450 activity in our permethrin-selected and nonselected strains from Mexico compared to the control strain. Our results suggest the Ae. albopictus sampled from 2016 are mostly susceptible to pyrethroids. These results contrast with the high levels of permethrin resistance (>58-fold) found in Ae. aegypti from the same sites in Mexico. This research indicates the importance of continued monitoring of Ae. albopictus populations to prevent resistance from developing in the future.
['Janich|Ashley J|AJ|', 'Saavedra-Rodriguez|Karla|K|', 'Vera-Maloof|Farah Z|FZ|', 'Kading|Rebekah C|RC|', 'Rodríguez|Américo D|AD|', 'Penilla-Navarro|Patricia|P|', 'López-Solis|Alma D|AD|', 'Solis-Santoyo|Francisco|F|', 'Perera|Rushika|R|', 'Black|William C|WC|']
D000330:Aedes D000818:Animals D017344:Genes, Insect D019476:Insect Proteins D007305:Insecticide Resistance D007306:Insecticides D008800:Mexico D009032:Mosquito Control D000072138:Mosquito Vectors D009154:Mutation D026023:Permethrin D011722:Pyrethrins D000079426:Vector Borne Diseases D061566:Voltage-Gated Sodium Channels
permethrin resistance mosquitoes