Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs.
The airway mucosa and the alveolar surface form dynamic interfaces between the lung and the external environment. The epithelial cells lining these barriers elaborate a thin liquid layer containing secreted peptides and proteins that contribute to host defense and other functions. The goal of this study was to develop and apply methods to define the proteome of porcine lung lining liquid, in part, by leveraging the wealth of information in the Sus scrofa database of Ensembl gene, transcript, and protein model predictions. We developed an optimized workflow for detection of secreted proteins in porcine bronchoalveolar lavage (BAL) fluid and in methacholine-induced tracheal secretions [airway surface liquid (ASL)]. We detected 674 and 3,858 unique porcine-specific proteins in BAL and ASL, respectively. This proteome was composed of proteins representing a diverse range of molecular classes and biological processes, including host defense, molecular transport, cell communication, cytoskeletal, and metabolic functions. Specifically, we detected a significant number of secreted proteins with known or predicted roles in innate and adaptive immunity, microbial killing, or other aspects of host defense. In greatly expanding the known proteome of the lung lining fluid in the pig, this study provides a valuable resource for future studies using this important animal model of pulmonary physiology and disease.
['Bartlett|Jennifer A|JA|', 'Albertolle|Matthew E|ME|', 'Wohlford-Lenane|Christine|C|', 'Pezzulo|Alejandro A|AA|', 'Zabner|Joseph|J|', 'Niles|Richard K|RK|', 'Fisher|Susan J|SJ|', 'McCray|Paul B|PB|', 'Williams|Katherine E|KE|']
D000818:Animals D000831:Animals, Newborn D001826:Body Fluids D001992:Bronchoalveolar Lavage Fluid D030562:Databases, Protein D004847:Epithelial Cells D008168:Lung D013058:Mass Spectrometry D016210:Methacholine Chloride D020543:Proteome D020545:Respiratory Mucosa D034421:Sus scrofa D014132:Trachea
bronchoalveolar lavage airway surface liquid proteomics porcine lung