Anti-obesity Effect of Halophyte Crop, Limonium tetragonum in High-Fat Diet-Induced Obese Mice and 3T3-L1 Adipocytes.
Halophyte Limonium tetragonum has recently been of interest in Korea for its nutritional value and salty taste which made it an ideal vegetable. In this study, the potential of L. tetragonum preventing excess weight gain, obesity and the related health problem has been evaluated in vitro and in vivo. The treatment with 100 mg/kg of L. tetragonum EtOAc soluble fraction (EALT) apparently prevented the body weight gain, adipose tissue weight gain, and the increase of triglyceride and total cholesterol level in mice fed a high-fat diet for 8 weeks. In addition, both glucose tolerance and insulin resistance in dietary obese mice were improved by EALT administration. A marked decrease in adipocyte differentiation was observed in the EALT (50 µg/mL)-treated 3T3-L1 cells, which was mediated by the suppression of adipogenesis-related transcription factors including peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/enhancer binding protein (C/EBP)α, and Sterol regulatory element binding protein-1 (SREBP-1) and adipocyte-specific proteins such as fatty acid synthase (FAS), lipoprotein lipase (LPL), and adipocyte fatty acid-binding protein (aP2). The major components contained in EALT were identified as (-)-epigallocatechin-3-(3″-O-methyl) gallate, (-)-epigallocatechin-3-gallate, and myricetin-3-O-β-D-galactopyranoside based on its phytochemical analysis. Results suggested that EALT might be available as functional crop and bioactive diet supplement for the prevention and/or treatment of obesity.
['Kim|Na-Hyun|NH|', 'Heo|Jeong-Doo|JD|', 'Rho|Jung-Rae|JR|', 'Yang|Min Hye|MH|', 'Jeong|Eun Ju|EJ|']
D041721:3T3-L1 Cells D017667:Adipocytes D050156:Adipogenesis D000818:Animals D019440:Anti-Obesity Agents D001786:Blood Glucose D059305:Diet, High-Fat D019587:Dietary Supplements D004195:Disease Models, Animal D005951:Glucose Tolerance Test D006801:Humans D007333:Insulin Resistance D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D008820:Mice, Obese D009765:Obesity D064209:Phytochemicals D010936:Plant Extracts D029606:Plumbaginaceae D056910:Republic of Korea D014280:Triglycerides D015430:Weight Gain
Limonium tetragonum high-fat diet-induced obesity insulin resistance 3T3-L1 adipogenesis adipocyte-specific gene glucose intolerance