Characterization of estrogenic receptor agonists and evaluation of estrogenic activity in the sediments of Liaohe River protected areas.
Estrogenic activity of 12 sediment samples from Liaohe River protected areas was evaluated by the recombinant yeast bioassays. The bioassay-derived 17β-estradiol equivalents of crude extracts (Bio-EEQcrudes) were between 52.2 and 207.6pg/g dry weight. The most concerned estrogenic receptor (ER) agonists including estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethynylestradiol (EE2), 4-nonylphenols (4-NP), bisphenol A (BPA), and organochlorine pesticides (OCPs) were determined. The concentrations of E1, E2, E3, EE2, BPA, andΣ10OCPs ranged up to 203.3pg/g, 185.8pg/g, 237.7pg/g, 188.5pg/g, 51.0ng/g, and 3.6ng/g, respectively. Taken together with polarity-based fractionation, in vitro bioassay and chemical analysis, it indicated that E1, E2, and EE2 were the predominant ER agonists and were mainly from the discharge of domestic wastewater and breeding wastewater. Meanwhile, this study showed that the establishment of protected areas had not obviously reduced the ecological risk caused by ER agonists in Liaohe River protected areas sediments.
['Ke|Xin|X|', 'Wang|Chunyong|C|', 'Zhang|Haijun|H|', 'Zhang|Yun|Y|', 'Gui|Shaofeng|S|']
D001559:Benzhydryl Compounds D001681:Biological Assay D002681:China D054750:Ecotoxicology D052244:Endocrine Disruptors D004958:Estradiol D004964:Estriol D004967:Estrogens D004970:Estrone D004997:Ethinyl Estradiol D019015:Geologic Sediments D006843:Hydrocarbons, Chlorinated D010575:Pesticides D010636:Phenols D045483:Rivers D062065:Waste Water D014874:Water Pollutants, Chemical D015003:Yeasts
Sediments Estrogen receptor (ER) agonists Polarity-based fractionation In vitro bioassays Chemical analysis