Purification of Circulating Cell-Free DNA from Plasma and Urine Using the Automated Large-Volume Extraction on the QIAsymphony® SP Instrument.
Increasing sample numbers for screening and diagnostics using circulating cell-free DNA (ccfDNA) as analyte demands an automated solution for ccfDNA extraction. The efficiency of a new, automated, large volume ccfDNA extraction method was evaluated against a manual reference method. The new kit for automated ccfDNA extraction on the QIAsymphony showed a comparable yield of total ccfDNA from healthy donors as well as a comparable recovery of circulating cancer and fetal DNA. In conclusion, a new kit for automated ccfDNA extraction was established successfully.
['Wolf|Alexander|A|', 'Beller|Katharina|K|', 'Groemminger|Sebastian|S|', 'Hofmann|Wera|W|', 'Sachse|Matthias|M|', 'Fassunke|Jana|J|']
D001331:Automation D004247:DNA D005333:Fetus D064368:Healthy Volunteers D006801:Humans D016133:Polymerase Chain Reaction D011933:Reagent Kits, Diagnostic D015203:Reproducibility of Results
Automation QIAsymphony ccfDNA Cancer Fetal QIAGEN