Identification of BRCA1/2 p.Ser1613Gly, p.Pro871Leu, p.Lys1183Arg, p.Glu1038Gly, p.Ser1140Gly, p.Ala2466Val, p.His2440Arg variants in women under 45 years old with breast nodules suspected of having breast cancer in Burkina Faso.
Breast cancer is the top cause of cancer mortality among women in the world and the second in Africa. The aims of this study were to: i) identify women with breast nodules suspected of having breast cancer ii) sequence the BRCA1 and BRCA2 genes and iii) screen mutations. From 2015 to 2016, 112 women aged from 35 to 44 years, who had come for consultation in the gynecology/obstetrics and the oncology department of the University Hospital Yalgado Ouedraogo, voluntarily agreed to participate to this study. Whole blood was collected from those with mammary nodules. The genomic DNA was extracted using Qiagen kit. FAST KAPA was used for genomic DNA amplification and the purified PCR products were analyzed by direct sequencing using Big Dye v1.1 and ABI 3730 automated sequencer. Nucleotides substitutions were determined. We identified BRCA1 SNPs rs1799966, rs799917, rs16942, rs16941, rs2227945, and BRCA2 SNPs rs169547, rs4986860. These identified variants are found mostly in cases of benign tumors of breast or ovarian cancer with familial history of breast cancer. This study in Burkina-Faso, is the basis for improved and more specific genetic testing, and suggests that additional genes contributing to an increased risk of breast cancer should be analyzed.
['Sagna|Tani|T|', 'Bonora|Elena|E|', 'Ouedraogo|Marie Nabonswindé Lamoussa|MNL|', 'Fusco|Daniela|D|', 'Zoure|Abdou Azaque|AA|', 'Bisseye|Cyrille|C|', 'Djigma|Florencia|F|', 'Kafando|Jacques Gilbert|JG|', 'Zongo|Nayi|N|', 'Douamba|Zoenabo|Z|', 'Obiri-Yeboah|Dorcas|D|', 'Turchetti|Daniela|D|', 'Pietra|Virginio|V|', 'Lompo|Olga Melanie|OM|', 'Ouedraogo|Charlemagne|C|', 'Seri|Marco|M|', 'Simpore|Jacques|J|']
D000328:Adult D019313:BRCA1 Protein D024682:BRCA2 Protein D001943:Breast Neoplasms D002050:Burkina Faso D005260:Female D005820:Genetic Testing D006801:Humans D042361:Mammary Glands, Human D020125:Mutation, Missense D020641:Polymorphism, Single Nucleotide
Breast cancer Burkina Faso SNPs BRCA Polymorphism