An Enzyme-Linked Aptamer Sorbent Assay to Evaluate Aptamer Binding.
Nucleic acid aptamers are a class of alternative ligands increasingly growing in importance in the face of contemporary detection challenges. Aptamers offer multiple advantages over traditional ligands like antibodies; however, their ability to specifically bind target molecules must first be confirmed after their generation. Use of a plate-based enzyme-linked aptamer sorbent assay (ELASA) is a generally rapid way to screen and characterize aptamer binding to protein targets. ELASA involves directly plating a protein target onto a nonspecific (polystyrene) surface and assessing binding of functionalized (biotinylated) aptamers to those plated proteins using an enzyme conjugate that recognizes the aptamers. Here, we describe an ELASA that was designed and used to evaluate and compare binding of ssDNA aptamers against the capsids of different strains of human norovirus.
['Moore|Matthew D|MD|', 'Escudero-Abarca|Blanca I|BI|', 'Jaykus|Lee-Ann|LA|']
D052157:Aptamers, Nucleotide D001710:Biotin D036022:Capsid Proteins D004277:DNA, Single-Stranded D004797:Enzyme-Linked Immunosorbent Assay D006801:Humans D029322:Norovirus D052156:SELEX Aptamer Technique
Enzyme-linked aptamer sorbent assay Aptamer Aptamer binding Binding evaluation DNA-protein binding Plate assay