Alpha 1-antitrypsin inhibits microglia activation and facilitates the survival of iPSC grafts in hypertension mouse model.
This study was conducted to investigate the use of Alpha 1-antitrypsin (AAT) to inhibit microglia activation in chronic hypertension model and provide a permissive environment for stem cell transplantation. Chronic ocular hypertension of C57BL/6 mice using magnetic microbead injection was induced 3 weeks prior to iPSCs transplantation. The ocular hypertension model was assessed histologically and intraocular pressure was measured. Survival of grafted cells and microglia activation were examined by flow cytometry and immunofluorescence in AAT and PBS treated hosts. Retinal cytokines expression was also detected by real-time PCR. Chronic ocular hypertension resulted in persistent microglia activation and stem cell grafts loss. AAT treatment significantly inhibited microglia activation and facilitated the survival of transplant iPSCs 4w post transplantation compared to PBS treatment. AAT holds tremendous potential for the clinical application to control neuroinflammation factor in glaucoma and improve the stem cell replacement therapy of retinal neurodegenerative disease.
['Yang|Sijing|S|', 'Xian|Bikun|B|', 'Li|Kaijing|K|', 'Luo|Ziming|Z|', 'Liu|Yuchun|Y|', 'Hu|Dongpeng|D|', 'Ge|Jian|J|']
D000818:Animals D002470:Cell Survival D004195:Disease Models, Animal D006801:Humans D006973:Hypertension D057026:Induced Pluripotent Stem Cells D007429:Intraocular Pressure D008262:Macrophage Activation D051379:Mice D008810:Mice, Inbred C57BL D017628:Microglia D019636:Neurodegenerative Diseases D015213:Neuroimmunomodulation D009798:Ocular Hypertension D033581:Stem Cell Transplantation D000515:alpha 1-Antitrypsin
Alpha 1-antitrypsin Microglia iPSC grafts Hypertension mouse model Neuroinflammation