In Vivo Fluoride Ion Detection and Imaging in Mice Using a Designed Near-Infrared Ratiometric Fluorescent Probe Based on IR-780.
A ratiometric near-infrared fluorescence probe based on IR-780 is developed and applied to fluoride anion (F-) detection in potable water and white flour as well as fluorescence imaging in living cells and mice. The proposed probe not only displays a linear ratiometric (F740/F690 nm) fluorescence response but also possesses near-infrared wavelengths to F- with a detection limit of 0.2 μM. Moreover, the designed probe displays high selectivity toward F-, which makes it feasible for F- detection in potable water and white flour. More importantly, applied to monitor F- in living HepG2 cells and male BALB/c mice, the probe indicates good biocompatibility and low cytotoxicity. However, no study for F- detection has been reported by a ratiometric NIR fluorescent probe so far. We expect that this probe with superior properties has great potential for use in F- detection in biological systems and in vivo studies.
['Tian|Xinwei|X|', 'Tong|Xinxin|X|', 'Li|Zhao|Z|http://orcid.org/0000-0001-7702-3348', 'Li|Dongyu|D|', 'Kong|Qingjun|Q|', 'Yang|Xingbin|X|http://orcid.org/0000-0002-8039-0525']
D000818:Animals D060766:Drinking Water D005433:Flour D005456:Fluorescent Dyes D005459:Fluorides D056945:Hep G2 Cells D006801:Humans D007211:Indoles D007259:Infrared Rays D008297:Male D008807:Mice, Inbred BALB C
mice fluorescent probe fluoride anion imaging analysis