Determination of Six Pyrazole Fungicides in Grape Wine by Solid-Phase Extraction and Gas Chromatography-Tandem Mass Spectrometry.
A gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed for the first simultaneous identification and quantification of six pyrazole fungicides (furametpyr, rabenzazole, fluxapyroxad, penflufen, bixafen, and isopyrazam) in grape wine samples. The grape wine samples were first diluted with water, then purified by solid-phase extraction, and finally examined by GC-MS/MS in multiple reaction monitoring (MRM) mode. Matrix-matched calibration curves were used to correct the matrix effects. The limits of quantification (LOQs), calculated as 10 times the standard deviation, were 0.2-0.8 μg kg(-1) for the six pyrazole fungicides. The average recoveries were in the range of 74.3-94.5%, with relative standard deviations (RSDs) below 5.8%, measured at three concentration levels. The proposed method is suitable for the simultaneous determination of six pyrazole fungicides in grape wine samples.
['Shen|Yan|Y|', 'Li|Zhou|Z|', 'Ma|Qiang|Q|', 'Wang|Chuanxian|C|', 'Chen|Xiangzhun|X|', 'Miao|Qian|Q|', 'Han|Chao|C|']
D005659:Fungicides, Industrial D008401:Gas Chromatography-Mass Spectrometry D011720:Pyrazoles D012015:Reference Standards D052616:Solid Phase Extraction D014920:Wine
pyrazole fungicides grape wines GC−MS/MS SPE