Baicalein protects renal tubular epithelial cells againsthypoxia-reoxygenation injury.
BACKGROUND
To investigate the protective effects and mechanism of baicalein (BAI), a naturally occurring flavonoid, against hypoxia-reoxygenation (HR) injury in renal tubular epithelial cells (HK-2).


METHODS
Cultured human renal proximal tubular cell line HK-2 was exposed to 24 h of hypoxia (5% CO2, 1% O2, and 94% N2), followed by 12 h of reoxygenation (5% CO2, 21% O2, and 74% N2). HK-2 cells were divided into three groups: control, HR, and HR-BAI (0.3 µg/ml). Reactive oxygen species (ROS) were measured and cell apoptosis was analyzed by flow cytometry and morphology. ELISAs were performed to determine the levels of IL-1, intercellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1). IL-1β, ICAM-1, and MCP-1 mRNA levels were determined by real-time quantitative PCR.


RESULTS
HK-2 cells that underwent HR exhibited increases in IL-1β expression by 0.94%, ROS by 0.59%, ICAM-1 expression by 0.8%, and MCP-1 expression by 1.2%. Moreover, HK-2 cell apoptosis was increased after HR (p < .05). Compared with the HR group, BAI treatment reduced the elevation of oxidative stress (ROS) by 0.76%, as well as HR-mediated induction of IL-1β and apoptosis of HK2 cells. Protein and mRNA levels of ICAM-1 and MCP-1 were also reduced.


CONCLUSIONS
BAI protects renal tubular epithelial cells from HR injury by reducing inflammatory cytokine expression and oxidative stress.
['Chen|Chun|C|', 'Cai|Chudan|C|', 'Lin|Hanfei|H|', 'Zhang|Weidai|W|', 'Peng|Yanqiang|Y|', 'Wu|Kefei|K|']
D017209:Apoptosis D015687:Cell Hypoxia D002460:Cell Line D016207:Cytokines D004847:Epithelial Cells D044950:Flavanones D006801:Humans D007684:Kidney Tubules D018384:Oxidative Stress D020011:Protective Agents D017382:Reactive Oxygen Species D015427:Reperfusion Injury
Baicalein renal tubular epithelial cell hypoxia-reoxygenation oxidative stress inflammation