Metabolomics study of cerebrospinal fluid from diabetic rats with cognitive impairment simultaneously treated with Panax quinquefolius and Acorus gramineus.
A metabolomics approach was used to explore the effects of Panax quinquefolius (PQ) and Acorus gramineus (AG) on learning and memory in rats with diabetic-induced cognitive impairment. Thirty Wistar rats were divided into three groups, namely, the normal group, model group, and PQ-AG group (PQ-AG group, 1.80 g/kg/d). Diabetes was induced by intraperitoneal injection of streptozotocin (65 mg/kg). Cerebrospinal fluid (CSF) was collected via cisterna magna puncture, and the Morris water maze method was used to evaluate learning and memory in rats after 11 weeks of PQ-AG treatment. Metabolic profiling of CSF samples was performed by using UPLC-Q-TOF-MS. Compared with the normal group, the escape latency of the Morris water maze was significantly prolonged in model group rats after 12 weeks (p < 0.01). Compared with the model group, however, the escape latency was significantly shortened in PQ-AG group rats (p < 0.05). In multivariate statistical analysis, we identified 33 potential biomarkers, and six biomarkers were altered by PQ-AG. These biomarkers were involved in the metabolism of pyrimidine; nicotinate, and nicotinamide; glycine, serine, and threonine; and ascorbate and aldarate. Taken collectively, our results indicate that PQ-AG can attenuate diabetic-induced cognitive impairment by affecting a variety of metabolic pathways. Our results provide an experimental basis for studying the mechanism of action of PQ-AG.
['Yang|Yang|Y|', 'Wang|Dongxue|D|', 'Zhao|Ying|Y|https://orcid.org/0000-0003-0411-3742', 'Wang|Yue|Y|', 'Bi|Yuying|Y|', 'Bi|Tiantian|T|']
D029023:Acorus D000818:Animals D015415:Biomarkers D060825:Cognitive Dysfunction D003921:Diabetes Mellitus, Experimental D008297:Male D055442:Metabolome D055432:Metabolomics D005894:Panax D010936:Plant Extracts D025341:Principal Component Analysis D051381:Rats D017208:Rats, Wistar
metabolomics Panax quinquefolius Acorus gramineus diabetic-induced cognitive impairment pharmacodynamics