Nano-encapsulation of epigallocatechin gallate in the ferritin-chitosan double shells: Simulated digestion and absorption evaluation.
In this work, a double shell material chitosan (CS)-recombinant soybean seed H-2 ferritin (H-2F) was fabricated to encapsulate epigallocatechin gallate (EGCG) molecules. EGCG-loaded H-2F complex (EHF) was firstly prepared by taking advantages of the reversible self-assembly of the ferritin, and the EHF-CS composite (EHFC) was fabricated by electrostatic interactions with binding number n of (4.1 ± 0.11) and binding constant K of ((5.3 ± 0.2) × 105 M-1), respectively. It was calculated that about 12.6 of EGCG molecules can be encapsulated in one H-2F ferritin cage with an encapsulation efficiency of 9.69% (w/w). SDS-PAGE analysis indicated that the CS binding to H-2F could inhibit ferritin degradation by pepsin and trypsin; the stability of EGCG molecules in EHFC was also significantly improved in simulated gastrointestinal tract. In addition, the chitosan-ferritin double shells were beneficial for the transport of EGCG across the Caco-2 monolayer model based on ferritin uptake. This work demonstrates a novel method to promote stabilization and absorption of food bioactive molecules.
['Yang|Rui|R|', 'Liu|Yuqian|Y|', 'Gao|Yunjing|Y|', 'Yang|Zhiying|Z|', 'Zhao|Siqi|S|', 'Wang|Yongjin|Y|', 'Blanchard|Chris|C|', 'Zhou|Zhongkai|Z|']
D018938:Caco-2 Cells D002392:Catechin D048271:Chitosan D004063:Digestion D004591:Electrophoresis, Polyacrylamide Gel D005293:Ferritins D005750:Gastric Juice D006801:Humans D007408:Intestinal Absorption D007419:Intestinal Secretions D046529:Microscopy, Electron, Transmission D053758:Nanoparticles D036103:Nanotechnology D010434:Pepsin A D013050:Spectrometry, Fluorescence D055672:Static Electricity D014357:Trypsin
Epigallocatechin gallate Digestion Ferritin Absorption by Caco-2 cell Nanocarrier