Indoleamine 2,3-dioxygenase (IDO) activity in placental compartments of renal-transplanted pregnant women.
PROBLEM
Immunosuppressive drugs change gestational IDO activity at the maternal-fetal interface.


METHOD OF STUDY
Analysis of placental IDO expression and activity, interferon gamma (IFN-γ), and IL-10 expression and NFkB activity in renal transplant recipient women under immunosuppressive treatment.


RESULTS
We demonstrated a significant reduction in IDO activity (P = 0.0275) and expression (P = 0.026) and in NFkB activity (P = 0.0176) in the villous region of renal transplanted mother. These findings did not correlate with the higher serum levels of kynurenine (P = 0.002). In the decidual compartment, IDO was immunolocalized mainly on the extravillous cytotrophoblast but did not show significant differences among the experimental groups; kynurenine was significantly higher (P = 0.036) and was inversely proportional to the decidual IFN-γ profile (P = 0.0433). No change was seen in IL-10 levels. NFkB activity was significantly higher in decidual compartment correlating with the higher IDO activity and suggesting that in immunosuppressant pregnancy, IDO activity and expression remain regulated by NFkB.


CONCLUSION
The increased IDO activity in decidua may indicate an attempt to offset the low expression. These findings call attention to the relevance of IDO activity at the maternal interface in pregnant transplant recipients, likely modulated by immunosuppressive agents and associated with a high risk of associated gestational disorders.
['do Prado|Karen Matias|KM|', 'Correa-Silva|Simone|S|', 'Oliveira|Leandro Gustavo|LG|', 'Camara|Niels Olsen Saraiva|NO|', 'Ono|Erica|E|', 'Sandri|Silvana|S|', 'Tourino|Melissa Cavalheiro|MC|', 'Campa|Ana|A|', 'de Sá Lima|Larissa|L|', 'Scavone|Cristoforo|C|', 'Bevilacqua|Estela|E|']
D000328:Adult D002851:Chromatography, High Pressure Liquid D024202:Electrophoretic Mobility Shift Assay D004797:Enzyme-Linked Immunosorbent Assay D005260:Female D006801:Humans D016867:Immunocompromised Host D007150:Immunohistochemistry D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase D007371:Interferon-gamma D016030:Kidney Transplantation D016328:NF-kappa B D010920:Placenta D011247:Pregnancy D011248:Pregnancy Complications
IDO Immunosuppressive drugs IFN-γ Kynurenine NF-κB Placenta