ROS-Responsive Nanoparticle as a Berberine Carrier for OHC-Targeted Therapy of Noise-Induced Hearing Loss.
Overproduction of reactive oxygen species (ROS) and inflammation are two key pathogeneses of noise-induced hearing loss (NIHL), which leads to outer hair cell (OHC) damage and hearing loss. In this work, we successfully developed ROS-responsive nanoparticles as berberine (BBR) carriers (PL-PPS/BBR) for OHC-targeted therapy of NIHL: Prestin-targeting peptide 2 (PrTP2)-modified nanoparticles (PL-PPS/BBR), which effectively accumulated in OHC areas, and poly(propylene sulfide)120 (PPS120), which scavenged ROS and converted to poly(propylene sulfoxide)120 in a ROS environment to disintegrate and provoke the rapid release of BBR with anti-inflammatory and antioxidant effects. In this study, satisfactory anti-inflammatory and antioxidant effects of PL-PPS/BBR were confirmed. Immunofluorescence and scanning electron microscopy (SEM) images showed that PL-PPS/BBR effectively accumulated in OHCs and protected the morphological integrity of OHCs. The auditory brainstem response (ABR) results demonstrated that PL-PPS/BBR significantly improved hearing in NIHL guinea pigs after noise exposure. This work suggested that PL-PPS/BBR may be a new potential treatment for noise-associated injury with clinical application.
['Zhao|Zeqi|Z|', 'Han|Zhengzhong|Z|', 'Naveena|Konduru|K|', 'Lei|Guanxiong|G|', 'Qiu|Shiwei|S|', 'Li|Xuanyi|X|', 'Li|Ting|T|', 'Shi|Xi|X|', 'Zhuang|Wei|W|', 'Li|Yalan|Y|', 'Qiao|Yuehua|Y|', 'Liu|Hongmei|H|http://orcid.org/0000-0002-0473-0030']
D000818:Animals D000894:Anti-Inflammatory Agents, Non-Steroidal D000975:Antioxidants D001599:Berberine D004195:Disease Models, Animal D004337:Drug Carriers D016057:Evoked Potentials, Auditory, Brain Stem D006168:Guinea Pigs D018072:Hair Cells, Auditory, Outer D006317:Hearing Loss, Noise-Induced D015394:Molecular Structure D053758:Nanoparticles D010316:Particle Size D017382:Reactive Oxygen Species D013499:Surface Properties
berberine noise-induced hearing loss outer hair cells drug delivery radical oxygen species