Influence of different iodinated contrast media on the induction of DNA double-strand breaks after in vitro X-ray irradiation.
The objective of this work was to examine differences in DNA double-strand break induction in peripheral blood lymphocytes after in vitro X-ray irradiation between iodinated contrast agents. Four different iodinated X-ray contrast agents--three of them with two different iodine concentrations--and mannitol (negative control; concentration of 150 mg mannitol per ml blood) were pipetted into blood samples so that there was a concentration of 0, 7.5 or 15 mg of iodine per ml blood in the samples. Negative controls without contrast medium (0 mg of iodine per ml blood) were also processed for every irradiation dose. The tubes were exposed to 0, 20 or 500 mGy in vitro X-ray irradiation. After that, the lymphocytes were separated by using density-gradient centrifugation. Fluorescence microscopy was applied to determine the average number of γH2AX-foci per lymphocyte in the presence or absence of different contrast media or mannitol. Differences in the number of γH2AX-foci were statistically analysed by one-way ANOVA and post-hoc Tukey's honestly significant difference test. Iodinated contrast agents led to a statistically significant increase in DNA double-strand breaks after in vitro irradiation. This effect increased statistically significant with rising radiation dose and appeared independent of the contrast agent used (iopromid, iodixanol, iomeprol, iopamidol). A statistically significant difference in DNA damage between the different tested contrast agents was not found. Therefore, the increase in DNA double-strand breaks depends solely on the amount of iodine applied. For evaluation of clinical consequences, our findings could be tested in further animal studies.
['Deinzer|Christoph K W|CK|', 'Danova|Daniela|D|', 'Kleb|Beate|B|', 'Klose|Klaus J|KJ|', 'Heverhagen|Johannes T|JT|']
D000328:Adult D003287:Contrast Media D053903:DNA Breaks, Double-Stranded D004249:DNA Damage D006657:Histones D006801:Humans D007455:Iodine D008214:Lymphocytes D008297:Male D008353:Mannitol D008856:Microscopy, Fluorescence D014057:Tomography, X-Ray Computed D014965:X-Rays
iodinated contrast media DNA double-strand breaks fluorescence microscopy γH2AX-foci experimental studies radiation damage