An expression atlas of miRNAs in Arabidopsis thaliana.
MicroRNAs (miRNAs) are small non-coding RNAs that regulate a variety of biological processes. MiRNA expression often exhibits spatial and temporal specificity. However, genome-wide miRNA expression patterns in different organs during development of Arabidopsis thaliana have not yet been systemically investigated. In this study, we sequenced small RNA libraries generated from 27 different organ/tissue types, which cover the entire life cycle of Arabidopsis. Analysis of the sequencing data revealed that most miRNAs are ubiquitously expressed, whereas a small set of miRNAs display highly specific expression patterns. In addition, different miRNA members within the same family have distinct spatial and temporal expression patterns. Moreover, we found that some miRNAs are produced from different arms of their hairpin precursors at different developmental stages. This work provides new insights into the regulation of miRNA biogenesis and a rich resource for future investigation of miRNA functions in Arabidopsis.
['Xu|Le|L|', 'Hu|Yugang|Y|', 'Cao|Ying|Y|', 'Li|Jingrui|J|', 'Ma|Ligeng|L|', 'Li|Yan|Y|', 'Qi|Yijun|Y|']
D017360:Arabidopsis D019295:Computational Biology D020869:Gene Expression Profiling D018507:Gene Expression Regulation, Developmental D018506:Gene Expression Regulation, Plant D015723:Gene Library D018745:Genome, Plant D035683:MicroRNAs D009928:Organ Specificity D018749:RNA, Plant
miRNA high-throughput sequencing plant development