FoxP3+ T regulatory cells and immunomodulation after Schistosoma mansoni egg antigen immunization in experimental model of inflammatory bowel disease.
To assess the effect of Schistosoma mansoni egg antigen immunization on the immunomodulation in dextran sodium sulfate (DSS) induced colitis as an experimental model of IBD in comparison to non immunization and healthy control. The study was performed on 180 mice; 25 healthy control, 15 to identify the inflammatory peak of DSS, 25 received DSS for 7 days; 90 infected with S. mansoni cercariae to collect eggs for antigen preparation, and 25 immunized with the prepared antigen then received DSS course. Disease activity index, macroscopic & microscopic inflammatory scores, FoxP3+ T regulatory cell count, myeloperoxidase activity, and Th1/Th2 cytokine profile were compared in studied groups. Immunization induced both FoxP3+ T(regs) and Th2 cytokines to establish a state of immune homeostasis and create a quiescent steadier immune response to DSS. S. mansoni egg antigen succeeded in acting like a prophylactic helminthic therapy as it has a profitable modulatory effect on DSS-induced colitis model.
['Hasby|Eiman A|EA|', 'Hasby Saad|Marwa A|MA|', 'Shohieb|Zeinab|Z|', 'El Noby|Kholoud|K|']
D000818:Animals D000947:Antigens, Helminth D016207:Cytokines D016264:Dextran Sulfate D004797:Enzyme-Linked Immunosorbent Assay D005260:Female D051858:Forkhead Transcription Factors D007114:Immunization D007150:Immunohistochemistry D056747:Immunomodulation D015212:Inflammatory Bowel Diseases D018655:Lymphocyte Count D051379:Mice D010063:Ovum D009195:Peroxidase D012550:Schistosoma mansoni D012720:Severity of Illness Index D050378:T-Lymphocytes, Regulatory D018417:Th1 Cells D018418:Th2 Cells
FoxP3 T regulatory cells Schistosoma mansoni egg antigen Helminthic therapy DSS-induced colitis