Inhibition of Heat-shock Protein 27 Reduces 5-Fluorouracil-acquired Resistance in Human Colon Cancer Cells.
BACKGROUND/AIM
In previous work we showed that expression of heat-shock protein 27 (HSP27; encoded by HSPB1) was associated with inherent resistance to 5-fluorouracil (5-FU). However, the relationship between HSP27 and acquired resistance remains unknown.


MATERIALS AND METHODS
We generated an acquired resistance model (WiDr-R) of a colon cancer cell line by exposing WiDr cells to 5-FU. Cell viability assays under treatment with 5-FU, as well as down-regulation of HSP27 using small interfering HSP27 RNA, were performed. HSP27 mRNA and protein expression was analyzed using real-time polymerase chain reaction and western blotting.


RESULTS
5-FU-acquired resistance induced overexpression of HSP27 mRNA and protein levels in WiDr-R cells. Furthermore, siRNA knockdown of HSP27 in WiDr-R cells reduced 5-FU-acquired resistance.


CONCLUSION
These findings demonstrate that HSP27 is associated with 5-FU resistance in human colon cancer cell cells and suggest that HSP27 regulation represents a novel approach to overcoming chemoresistance in colorectal cancer.
['Asada|Yusuke|Y|', 'Tsuruta|Masashi|M|', 'Okabayashi|Koji|K|', 'Shigeta|Kohei|K|', 'Ishida|Takashi|T|', 'Shimada|Takehiro|T|', 'Suzumura|Hirofumi|H|', 'Koishikawa|Kaoru|K|', 'Akimoto|Shingo|S|', 'Hasegawa|Hirotoshi|H|', 'Kitagawa|Yuko|Y|']
D045744:Cell Line, Tumor D003110:Colonic Neoplasms D019008:Drug Resistance, Neoplasm D005472:Fluorouracil D055551:HSP27 Heat-Shock Proteins D006801:Humans
Heat-shock protein 27 colorectal cancer 5-fluorouracil resistance chemotherapy