Planomicrobium iranicum sp. nov., a novel slightly halophilic bacterium isolated from a hypersaline wetland.
A novel strain, designated as MX6T was isolated from Meighan wetland, in the centre of Iran. The cells were Gram-stain-positive, motile, coccoid to rod-shaped, oxidase- and catalase-positive. The strain grew optimally at 35 °C, 3 % (w/v) NaCl and pH 7-7.5. A polyphasic taxonomic study was undertaken in order to characterize the strain in detail. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that MX6T represented a member of the phylum Firmicutes, family Planococcaceae, genus Planomicrobium, and showed the highest similarity with Planomicrobium flavidum ISL-41T (98.2 %) and Planomicrobium psychrophilum CMC 53orT (98.0 %). The main polar lipids of MX6T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and seven unidentified phospholipids and its DNA G+C content was 45.5 mol%. Major cellular fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol, iso-C14 : 0, iso-C15 : 0 and iso-C16 : 0 and the predominant respiratory quinone was Q-8 (62 %). Experimental DNA-DNA hybridization between MX6T and Planomicrobium flavidum IBRC-M 11047T was 20 %, supporting the differential taxonomic status of MX6T as representing a different taxon. All these data indicate that MX6T represents a novel species of the genus Planomicrobium, for which the name Planomicrobium iranicum sp. nov. is proposed. The type strain is MX6T (=IBRC M 10928T=LMG 28548T).
['Ramezani|Mohaddaseh|M|', 'Nikou|Mahdi Moshtaghi|MM|', 'Pourmohyadini|Mohammad|M|', 'Spröer|Cathrin|C|', 'Schumann|Peter|P|', 'Harirchi|Shrareh|S|', 'Aryaefar|Mahnaz|M|', 'Fazeli|Seyed Abolhassan Shahzadeh|SAS|', 'Amoozegar|Mohammad Ali|MA|', 'Ventosa|Antonio|A|']
D001406:Bacillaceae D015373:Bacterial Typing Techniques D001482:Base Composition D004269:DNA, Bacterial D005227:Fatty Acids D007492:Iran D009693:Nucleic Acid Hybridization D010743:Phospholipids D010802:Phylogeny D058641:Planococcaceae D012336:RNA, Ribosomal, 16S D054712:Salinity D017422:Sequence Analysis, DNA D014451:Ubiquinone D053833:Wetlands
slightly halophilic bacterium hypersaline wetland Gram-stain-positive