Construction of hybrid DNAs@CP for the rapid synchronous sensing of multiplex microRNAs based on experimental studies and molecular simulation.
A water stable one-dimensional (1D) ladder-shaped coordination polymer (CP) has been synthesized and exhibits a strong affinity to two fluorescein-tagged single-stranded probe DNAs (P-DNAs), giving a sensing platform of P-DNAs@1. Such a hybrid sensing platform is capable of simultaneous detection of breast cancer related microRNA-221 (miRNA-221) and miRNA-222 in a specific and synchronous manner, without observable cross-reactions, as supported by experimental evidences. The interaction mode and the electronic energy between CP 1 with nucleic acid were confirmed by molecular simulation and the universal force field (UFF).
['Yang|Zi-Chuan|ZC|', 'Wang|Tao-Rui|TR|', 'Li|Rong-Tian|RT|', 'Chen|Ting-Wei|TW|', 'Fan|Yue|Y|', 'Jiang|Wei-Hua|WH|', 'Li|Min-Min|MM|', 'Chen|Jin-Xiang|JX|']
D056831:Coordination Complexes D004247:DNA D035683:MicroRNAs D056004:Molecular Dynamics Simulation
Rapid synchronous sensing Molecular simulation Coordination polymer miRNA sensing