Application of a highly sensitive magnetic solid phase extraction for phytochemical compounds in medicinal plant and biological fluids by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry.
A highly sensitive method using reduced graphene oxide with iron oxide (rGO/Fe3 O4 ) as the sorbent in magnetic SPE has been developed for the purification of five anthraquinones (emodin, rhein, aloeemodin, physcion, and chrysophanol) in rhubarb and rat urine by ultra-HPLC coupled with quadrupole TOF/MS. The extraction was accomplished by adding trace amount rGO/Fe3 O4 suspension to 200 mL of aqueous mixture, and the excellent adsorption capacity of the nanoparticles was fully demonstrated in this procedure. Under the optimized conditions, the calibration curves were linear in the concentration range of 0.05-27.77 ng/mL with correlation coefficients varying from 0.9902 to 0.9978. The LODs ranged from 0.28 to 58.99 pg/mL. The experimental results indicated that the proposed method was feasible for the analysis of anthraquinones in rhubarb and urine samples.
['Cao|Wan|W|', 'Yi|Ling|L|', 'Ye|Li-Hong|LH|', 'Cao|Jun|J|', 'Hu|Shuai-Shuai|SS|', 'Xu|Jing-Jing|JJ|', 'Peng|Li-Qing|LQ|', 'Zhu|Qiong-Yao|QY|', 'Zhang|Qian-Yun|QY|']
D000818:Animals D000880:Anthraquinones D002851:Chromatography, High Pressure Liquid D006863:Hydrogen-Ion Concentration D057230:Limit of Detection D016014:Linear Models D058185:Magnetite Nanoparticles D008297:Male D064209:Phytochemicals D010946:Plants, Medicinal D051381:Rats D017207:Rats, Sprague-Dawley D015203:Reproducibility of Results D052616:Solid Phase Extraction D053719:Tandem Mass Spectrometry
Magnetic SPE Anthraquinones Rhubarb Rat urine Ultra-HPLC coupled with quadrupole TOF/MS