Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver.
Microcystins (MCs) are common toxins produced by freshwater cyanobacteria, and they represent a potential health risk to aquatic organisms and animals, including humans. Specific inhibition of protein phosphatases 1 and 2A is considered the typical mechanism of MCs toxicity, but the exact mechanism has not been fully elucidated. To further our understanding of the toxicological mechanisms induced by MCs, this study is the first to use a chemical proteomic approach to screen proteins that exhibit special interactions with MC-arginine-arginine (MC-RR) from zebrafish (Danio rerio) liver. Seventeen proteins were identified via affinity blocking test. Integration of the results of previous studies and this study revealed that these proteins play a crucial role in various toxic phenomena of liver induced by MCs, such as the disruption of cytoskeleton assembly, oxidative stress, and metabolic disorder. Moreover, in addition to inhibition of protein phosphate activity, the overall toxicity of MCs was simultaneously modulated by the distribution of MCs in cells and their interactions with other target proteins. These results provide new insight into the mechanisms of hepatotoxicity induced by MCs. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1206-1216, 2016.
['Tuo|Xun|X|', 'Chen|Jun|J|', 'Zhao|Sujuan|S|', 'Xie|Ping|P|']
D000818:Animals D002851:Chromatography, High Pressure Liquid D000458:Cyanobacteria D003599:Cytoskeleton D015180:Electrophoresis, Gel, Two-Dimensional D006801:Humans D008099:Liver D008387:Marine Toxins D052998:Microcystins D018384:Oxidative Stress D054645:Protein Phosphatase 1 D054648:Protein Phosphatase 2 D020543:Proteome D040901:Proteomics D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization D015027:Zebrafish
chemical proteomics toxicological mechanisms microcystin-RR zebrafish liver