A (2)H magic-angle spinning solid-state NMR characterisation of lipid membranes in intact bacteria.
This work proposes a new approach to characterize cell membranes in intact cells by (2)H solid-state nuclear magnetic resonance (NMR) in only a few hours using magic-angle spinning (MAS) and spectral moment analysis. The method was first validated on model dipalmitoylphosphatidylcholine (DPPC) membranes, allowing the detection of lipid fluctuations below the main transition temperature. Then the lipid dynamics in Escherichia coli membranes was compared in bacteria grown under different diets. More specifically, deuterated palmitic acid was used to isotopically label the phospholipid acyl chains in bacteria membranes, with or without the presence of protonated oleic acid. Our results showed improved lipid fluidity when bacteria were grown in the presence of oleic acid, which helps preserving the natural fatty acid profile in E. coli membranes. The MAS (2)H solid-state NMR study of membranes combined with spectral moment analysis showed to be a fast method compatible with in vivo bacterial studies, and should also be applicable to other micro-organisms to obtain molecular information on living cells by solid-state NMR.
['Warnet|Xavier L|XL|', 'Laadhari|Marwa|M|', 'Arnold|Alexandre A|AA|', 'Marcotte|Isabelle|I|', 'Warschawski|Dror E|DE|']
D015060:1,2-Dipalmitoylphosphatidylcholine D003903:Deuterium D004926:Escherichia coli D008051:Lipid Bilayers D009682:Magnetic Resonance Spectroscopy D019301:Oleic Acid D019308:Palmitic Acid D013329:Structure-Activity Relationship D013816:Thermodynamics D044366:Transition Temperature
Lipids Bacteria Dynamics Deuterium NMR In cell solid-state nuclear magnetic resonance