IDENTIFICATION AND EXPRESSION ANALYSIS OF TWO 14-3-3 PROTEINS IN THE MOSQUITO Aedes aegypti, AN IMPORTANT ARBOVIRUSES VECTOR.
The 14-3-3 proteins are evolutionarily conserved acidic proteins that form a family with several isoforms in many cell types of plants and animals. In invertebrates, including dipteran and lepidopteran insects, only two isoforms have been reported. 14-3-3 proteins are scaffold molecules that form homo- or heterodimeric complexes, acting as molecular adaptors mediating phosphorylation-dependent interactions with signaling molecules involved in immunity, cell differentiation, cell cycle, proliferation, apoptosis, and cancer. Here, we describe the presence of two isoforms of 14-3-3 in the mosquito Aedes aegypti, the main vector of dengue, yellow fever, chikungunya, and zika viruses. Both isoforms have the conserved characteristics of the family: two protein signatures (PS1 and PS2), an annexin domain, three serine residues, targets for phosphorylation (positions 58, 184, and 233), necessary for their function, and nine alpha helix-forming segments. By sequence alignment and phylogenetic analysis, we found that the molecules correspond to Ɛ and ζ isoforms (Aeae14-3-3ε and Aeae14-3-3ζ). The messengers and protein products were present in all stages of the mosquito life cycle and all the tissues analyzed, with a small predominance of Aeae14-3-3ζ except in the midgut and ovaries of adult females. The 14-3-3 proteins in female midgut epithelial cells were located in the cytoplasm. Our results may provide insights to further investigate the functions of these proteins in mosquitoes.
['Trujillo-Ocampo|Abel|A|', 'Cázares-Raga|Febe Elena|FE|', 'Celestino-Montes|Antonio|A|', 'Cortés-Martínez|Leticia|L|', 'Rodríguez|Mario H|MH|', 'Hernández-Hernández|Fidel de la Cruz|FC|']
D048948:14-3-3 Proteins D000330:Aedes D000595:Amino Acid Sequence D000818:Animals D005260:Female D018507:Gene Expression Regulation, Developmental D019476:Insect Proteins D007303:Insect Vectors D007814:Larva D008297:Male D009928:Organ Specificity D010802:Phylogeny D016133:Polymerase Chain Reaction D011679:Pupa D012333:RNA, Messenger D016415:Sequence Alignment
expression 14-3-3 proteins Aedes aegypti RNA