Effect of Exogenous Nitro Oxide on Chilling Tolerance, Polyamine, Proline, and γ-Aminobutyric Acid in Bamboo Shoots (Phyllostachys praecox f. prevernalis).
The effects of exogenous nitro oxide (NO) on chilling resistance and the metabolism of polyamine, proline, and γ-aminobutyric acid of bamboo shoots were investigated. Bamboo shoots were dipped in 0.07 mM sodium nitroprusside (SNP) and stored at 1 °C for 56 days. During the storage, the development of chilling injury of SNP treated bamboo shoots was inhibited with decreased accumulation of malonaldehyde and electrical leakage. At the end of storage, the chilling injury incidence of treated bamboo shoots decreased by 37.9% while their malonaldehyde content and electrical leakage were 8.8% and 18.6% lower than that of the control, respectively. Interestingly, the endogenous NO, polyamines, γ-aminobutyric acid, and proline contents of treated bamboo shoot also significantly increased. Consistently, the metabolisms of these nitrogenous compounds were stimulated in treated bamboo shoots, according to their higher (20.2%-49.8%) related enzyme activities, including nitric oxide synthase, arginine decarboxylase, ornithine decarboxylase, glutamate decarboxylase, orn-δ-aminotransferase, and Δ1-pyrroline-5-carboxylate synthetase. The results indicated that the SNP treatment enhanced chilling tolerance of bamboo shoots, which might associate with the activated metabolism of polyamines, γ-aminobutyric acid, and proline. SNP treatment might be an alternative technology to avoid chill injury during cold storage of bamboo shoots.
['Wang|Di|D|', 'Li|Li|L|', 'Xu|Yanqun|Y|', 'Limwachiranon|Jarukitt|J|', 'Li|Dong|D|', 'Ban|Zhaojun|Z|', 'Luo|Zisheng|Z|http://orcid.org/0000-0001-8232-9739']
D002262:Carboxy-Lyases D003080:Cold Temperature D005968:Glutamate Decarboxylase D009569:Nitric Oxide D019001:Nitric Oxide Synthase D009955:Ornithine Decarboxylase D010940:Plant Proteins D018520:Plant Shoots D006109:Poaceae D011073:Polyamines D011392:Proline D005680:gamma-Aminobutyric Acid
nitro oxide polyamine proline bamboo shoot chilling injury GABA