Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination.
HSV glycoproteins play important roles in the viral life cycle, particularly viral cell entry. Here we describe the protocol for expression, purification, and crystallization of full-length HSV-1 glycoprotein B. The protocol provides a framework for incorporating transmembrane domain-stabilizing amphipols into the crystallization setup and can be adapted to isolate other complete HSV glycoproteins.
['Cooper|Rebecca S|RS|', 'Heldwein|Ekaterina E|EE|']
D000818:Animals D018360:Crystallography, X-Ray D015870:Gene Expression D018259:Herpesvirus 1, Human D000072417:Protein Domains D011994:Recombinant Proteins D061987:Sf9 Cells D018411:Spodoptera D014759:Viral Envelope Proteins
Glycoproteins Crystallography Ectodomain Herpes simplex viruses Membrane protein Protein purification Viral entry