Pseudogene integrator complex subunit 6 pseudogene 1 (INTS6P1) as a novel plasma-based biomarker for hepatocellular carcinoma screening.
In this study, we aimed to determine whether the pseudogene integrator complex subunit 6 pseudogene 1 (INTS6P1) in plasma could be used as a novel approach to screen for and detect hepatocellular carcinoma (HCC). We explored the clinical role of INTS6P1: First, the expression level of INTS6P1 was measured in a cohort of 33 HCC tissue samples and adjacent normal liver tissue, next, the INTS6P1 expression was detected in the culture medium and tumor cells in a cellular experiment, and last, the diagnostic performance of INTS6P1 was examined in an independent cohort of 100 people. The expression level of INTS6P1 was remarkably downregulated in the HCC tissues compared with that in the normal liver tissues (p = 0.0066). In plasma, the INTS6P1 levels were significantly decreased in HCC patients compared with non-HCC patients (p < 0.01). Additionally, we inferred that INTS6P1 might be a prospective biomarker for screening HCC patients in which the serum-AFP levels were lower than 20 ng/ml by the area under the curve-receiver operating characteristic (AUC-ROC) analysis (p < 0.05). Pseudogene INTS6P1 could be used as a novel HCC plasma-based biomarker and might improve the accuracy of HCC screening.
['Lui|Ka Yin|KY|', 'Peng|Hao-Ran|HR|', 'Lin|Jin-Rong|JR|', 'Qiu|Chun-Hui|CH|', 'Chen|Hu-An|HA|', 'Fu|Rong-Dang|RD|', 'Cai|Chang-Jie|CJ|', 'Lu|Min-Qiang|MQ|']
D000328:Adult D000368:Aged D019540:Area Under Curve D014408:Biomarkers, Tumor D006528:Carcinoma, Hepatocellular D045744:Cell Line, Tumor D015331:Cohort Studies D003470:Culture Media D005260:Female D056945:Hep G2 Cells D006801:Humans D008099:Liver D008113:Liver Neoplasms D008297:Male D008403:Mass Screening D008875:Middle Aged D020411:Oligonucleotide Array Sequence Analysis D011544:Pseudogenes D012334:RNA, Neoplasm D016601:RNA-Binding Proteins D012372:ROC Curve D012269:Ribosomal Proteins D012680:Sensitivity and Specificity D025521:Tumor Suppressor Proteins
Pseudogene INTS6P1 Hepatocellular carcinoma Plasma biomarker