Liquid Biopsy Targeting Monocarboxylate Transporter 1 on the Surface Membrane of Tumor-Derived Extracellular Vesicles from Synovial Sarcoma.
The lack of noninvasive biomarkers that can be used for tumor monitoring is a major problem for soft-tissue sarcomas. Here we describe a sensitive analytical technique for tumor monitoring by detecting circulating extracellular vesicles (EVs) of patients with synovial sarcoma (SS). The proteomic analysis of purified EVs from SYO-1, HS-SY-II, and YaFuSS identified 199 common proteins. DAVID GO analysis identified monocarboxylate transporter 1 (MCT1) as a surface marker of SS-derived EVs, which was also highly expressed in SS patient-derived EVs compared with healthy individuals. MCT1+CD9+ EVs were also detected from SS-bearing mice and their expression levels were significantly correlated with tumor volume (p = 0.003). Furthermore, serum levels of MCT1+CD9+ EVs reflected tumor burden in SS patients. Immunohistochemistry revealed that MCT1 was positive in 96.7% of SS specimens and its expression on the cytoplasm/plasma membrane was significantly associated with worse overall survival (p = 0.002). Silencing of MCT1 reduced the cellular viability, and migration and invasion capability of SS cells. This work describes a new liquid biopsy technique to sensitively monitor SS using circulating MCT1+CD9+ EVs and indicates the therapeutic potential of MCT1 in SS.
['Yokoo|Suguru|S|0000-0003-1678-8763', 'Fujiwara|Tomohiro|T|0000-0003-4257-9180', 'Yoshida|Aki|A|', 'Uotani|Koji|K|', 'Morita|Takuya|T|', 'Kiyono|Masahiro|M|', 'Hasei|Joe|J|', 'Nakata|Eiji|E|0000-0002-5971-4142', 'Kunisada|Toshiyuki|T|0000-0002-1775-850X', 'Iwata|Shintaro|S|0000-0002-2024-514X', 'Yonemoto|Tsukasa|T|', 'Ueda|Koji|K|', 'Ozaki|Toshifumi|T|']
liquid biopsy monocarboxylate transporter 1 extracellular vesicles synovial sarcoma non-invasive biomarker