Are there advantages in the use of specific pathogen-free baboons in pig organ xenotransplantation models?
Baboons have natural antibodies against pig antigens. We have investigated whether there are differences in anti-non-Gal pig antibody levels between baboons maintained under specific pathogen-free (SPF) conditions and those housed under conventional conditions (non-SPF) that might be associated with improved outcome after pig-to-baboon organ transplantation. Baboons (n = 40) were housed indoors (SPF n = 8) or in indoor/outdoor pens (non-SPF n = 32) in colonies of similar size and structure. Non-SPF colonies harbor a number of pathogens common to non-human primate species, whereas many of these pathogens have been eliminated from the SPF colony. Complete blood cell counts (CBC), blood chemistry, and anti-non-Gal IgM and IgG levels were monitored. There were no significant differences in CBC or blood chemistry between SPF and non-SPF baboons. Anti-non-Gal IgM levels were significantly lower in the SPF baboons than in the non-SPF baboons (MFI 7.1 vs. 8.8, P < 0.05). One SPF and two non-SPF baboons had an MFI >20; if these three baboons are omitted, the mean MFIs were 4.8 (SPF) vs. 7.5 (non-SPF) (P < 0.05). Anti-non-Gal IgG was minimal in both groups (MFI 1.0 vs. 1.0). As their levels of anti-non-Gal IgM are lower, baboons maintained under SPF conditions may be beneficial for xenotransplantation studies as the initial binding of anti-pig IgM to an α1,3-galactosyltransferase gene-knockout pig organ may be less, thus resulting in less complement and/or endothelial cell activation. However, even under identical SPF conditions, an occasional baboon will express a high level of anti-non-Gal IgM, the reason for which remains uncertain.
['Zhou|Huidong|H|', 'Iwase|Hayato|H|', 'Wolf|Roman F|RF|', 'Ekser|Burcin|B|', 'Ezzelarab|Mohamed|M|', 'Hara|Hidetaka|H|', 'White|Gary|G|', 'Cooper|David K C|DK|']
D000818:Animals D000910:Antibodies, Heterophile D015415:Biomarkers D005260:Female D007074:Immunoglobulin G D007075:Immunoglobulin M D008297:Male D023421:Models, Animal D010215:Papio D013047:Specific Pathogen-Free Organisms D013552:Swine D014183:Transplantation, Heterologous
specific pathogen-free baboon pig xenotransplantation antibody anti-pig α1,3-galactosyltransferase gene-knockout