Phylogenetic Analyses of Fowl Adenoviruses (FAdV) Isolated in China and Pathogenicity of a FAdV-8 Isolate.
Fowl adenoviruses (FAdVs) have a worldwide distribution and are associated with a variety of diseases, causing considerable economic losses to the poultry industry. We characterized 10 FAdVs isolated from China in 2015-2016 and assessed the pathogenicity of a FAdV-8 strain in specific-pathogen-free (SPF) chickens. Phylogenetic analysis of a hexon gene revealed that only 1 of the 10 isolates belonged to FAdV-8, whereas others belonged to FAdV-4, indicating that Chinese FAdVs were mainly FAdV-4 in recent years. The pathogenicity experiment of the FAdV-8 strain CH/SD/2015/09 showed that no clinical signs were observed in infected chickens. Necropsy displayed mild necrotic foci and petechial hemorrhage of livers collected at 5 days postinfection (dpi). Histopathologic examination identified the presence of intranuclear inclusion bodies in hepatocytes. No virus was detected in oral and cloacal swabs at 5 dpi, and only viral DNA could be measured in kidneys collected at the same time. The results revealed that CH/SD/2015/09 had no obvious pathogenicity in 5-wk-old SPF chickens, which could provide a better understanding about the pathogenicity of the FAdV-8 serotype.
['Ruan|Si-Fan|SF|', 'Zhao|Jing|J|', 'Ren|Ying-Chao|YC|', 'Feng|Jin-Ling|JL|', 'Zhang|Guo-Zhong|GZ|']
D000257:Adenoviridae Infections D000818:Animals D000259:Aviadenovirus D036022:Capsid Proteins D002645:Chickens D002681:China D008099:Liver D010802:Phylogeny D011201:Poultry Diseases D017422:Sequence Analysis, DNA D013047:Specific Pathogen-Free Organisms D014774:Virulence
pathogenicity FAdV-8 phylogenetic analysis hexon fowl adenovirus