P3H4 is correlated with clinicopathological features and prognosis in bladder cancer.
BACKGROUND
Genetic alterations play a significant role in the progression of bladder cancer. Identifying novel biomarkers to personalize the therapeutic regimen and evaluate the prognosis of patients with bladder cancer is vital. Prolyl 3-hydroxylase family member 4 (P3H4) is significantly involved in several types of human cancer. However, the effect of P3H4 in bladder cancer remains unknown.


METHODS
The mRNA expression of P3H4 was measured in 44 paired tumors and adjacent normal tissues by using real-time reverse transcription-polymerase chain reaction. RNA-Seq data of 389 patients with bladder cancer were downloaded to investigate the effect of P3H4 on bladder cancer from The Cancer Genome Atlas (TCGA) project.


RESULTS
P3H4 was overexpressed in bladder cancer compared with the adjacent normal tissue both in our tissue samples and TCGA samples. The mRNA expression of P3H4 was significantly related to several clinicopathological factors of bladder cancer, including age, race category, histologic grade, tumor histologic subtype, and AJCC stage. The high P3H4 expression group had a shorter overall survival (OS) than the low P3H4 expression group. Univariate Cox regression analysis showed that age, angiolymphatic invasion, lymph node metastasis, tumor histologic subtype, metastasis, AJCC stage, and P3H4 were significantly related to OS. Moreover, multivariate Cox analysis revealed that P3H4, as well as age and AJCC stage, was an independent predictor of poor OS.


CONCLUSION
Given its tumorigenic role, P3H4 may serve as a promising tumor-promoting gene in bladder cancer.
['Li|Wangjian|W|', 'Ye|Lihong|L|', 'Chen|Yongliang|Y|', 'Chen|Peng|P|http://orcid.org/0000-0001-8665-3990']
D000368:Aged D014408:Biomarkers, Tumor D016022:Case-Control Studies D005260:Female D005500:Follow-Up Studies D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008207:Lymphatic Metastasis D008297:Male D011393:Procollagen-Proline Dioxygenase D011379:Prognosis D060888:Real-Time Polymerase Chain Reaction D015996:Survival Rate D001749:Urinary Bladder Neoplasms
P3H4 Clinicopathological features Prognosis Bladder cancer