Unveiling the stimulatory effects of tartrazine on human and bovine serum albumin fibrillogenesis: Spectroscopic and microscopic study.
Amyloid fibrils are playing key role in the pathogenesis of various neurodegenerative diseases. Generally anionic molecules are known to induce amyloid fibril in several proteins. In this work, we have studied the effect of anionic food additive dye i.e., tartrazine (TZ) on the amyloid fibril formation of human serum albumins (HSA) and bovine serum albumin (BSA) at pHs7.4 and 3.5. We have employed various biophysical methods like, turbidity measurements, Rayleigh Light Scattering (RLS), Dynamic Light Scattering (DLS), intrinsic fluorescence, Congo red assay, far-UV CD, transmission electron microscopy (TEM) and atomic force microscopy (AFM) to decipher the mechanism of TZ-induce amyloid fibril formation in both the serum albumins at pHs7.4 and 3.5. The obtained results suggest that both the albumins forms amyloid-like aggregates in the presence of 1.0 to 15.0mM of TZ at pH3.5, but no amyloid fibril were seen at pH7.4. The possible cause of TZ-induced amyloid fibril formation is electrostatic and hydrophobic interaction because sulfate group of TZ may have interacted electrostatically with positively charged amino acids of the albumins at pH3.5 and increased protein-protein and protein-TZ interactions leading to amyloid fibril formation. The TEM, RLS and DLS results are suggesting that BSA forms bigger size amyloids compared to HSA, may be due to high surface hydrophobicity of BSA.
['Al-Shabib|Nasser Abdulatif|NA|', 'Khan|Javed Masood|JM|', 'Alsenaidy|Mohammad A|MA|', 'Alsenaidy|Abdulrahman M|AM|', 'Khan|Mohd Shahnawaz|MS|', 'Husain|Fohad Mabood|FM|', 'Khan|Mohammad Rashid|MR|', 'Naseem|Mohammad|M|', 'Sen|Priyankar|P|', 'Alam|Parvez|P|', 'Khan|Rizwan Hasan|RH|']
D002942:Circular Dichroism D003224:Congo Red D000067493:Dynamic Light Scattering D006801:Humans D057446:Hydrodynamics D006863:Hydrogen-Ion Concentration D057927:Hydrophobic and Hydrophilic Interactions D007700:Kinetics D018625:Microscopy, Atomic Force D008958:Models, Molecular D009391:Nephelometry and Turbidimetry D066329:Protein Aggregates D017433:Protein Structure, Secondary D012710:Serum Albumin, Bovine D000075462:Serum Albumin, Human D013645:Tartrazine
Human and bovine serum albumin Amyloid fibril Food additive dye Tartrazine (TZ) Protein aggregation pH