Tissue clearing and 3D imaging in developmental biology.
Tissue clearing increases the transparency of late developmental stages and enables deep imaging in fixed organisms. Successful implementation of these methodologies requires a good grasp of sample processing, imaging and the possibilities offered by image analysis. In this Primer, we highlight how tissue clearing can revolutionize the histological analysis of developmental processes and we advise on how to implement effective clearing protocols, imaging strategies and analysis methods for developmental biology.
['Vieites-Prado|Alba|A|0000-0002-8807-3321', 'Renier|Nicolas|N|0000-0003-2642-4402']
D000818:Animals D015509:Developmental Biology D006801:Humans D021621:Imaging, Three-Dimensional
Tissue clearing 3D imaging Light-sheet microscopy