Sodium Hypochlorite and a Preparation Containing Glycocholic Acid and Surfactants Have a Synergistic Action on Organic Tissue Dissolution In Vitro.
INTRODUCTION
The aim of this study was to evaluate porcine palatal mucosa dissolution by sodium hypochlorite (NaOCl) with or without an auxiliary dissolving agent containing glycocholic acid and a mixture of surfactants (Keratobacter [KB]; Saint Joseph DID, Valencia, Spain).


METHODS
One hundred forty samples were obtained from porcine palatal mucosa and weighed using a high-precision balance. The samples were randomly divided into 4 experimental groups (n = 35) based on the test solution used: distilled water (the negative control), CanalPro NaOCl 6% (Coltene Whaledent, Altstätten, Switzerland), KB, and a 9:1 vol/vol mixture of NaOCl with KB (NaOCl + KB). After 5, 10, 15, and 20 minutes of immersion in the solutions at 27°C ± 1°C, the samples were weighted by a blinded assessor. The intergroup weight at the different time points was statistically analyzed using the analysis of variance test with the Bonferroni posttest.


RESULTS
All test groups presented with tissue dissolution although complete dissolution did not occur in any sample. The largest percent in weight reduction occurred between time points (t) = 0 minutes and t = 5 minutes for the NaOCl + KB group (22.5%) followed by KB (18.5%) for the same time period. NaOCl presented with similar tissue dissolution activity during the different time points, ranging from 7.8% (t = 10 minutes-t = 15 minutes) to 6.8% (t = 15 minutes-t = 20 minutes). Significant weight differences were found among the different experimental groups after 5, 10, and 15 minutes of incubation, with the only exception being KB versus NaOCl + KB. No significant differences were found when comparing the test groups at t = 20 minutes.


CONCLUSIONS
The addition of KB to NaOCl increased porcine palatal mucosa dissolution in vitro.
['Peña López|Alejandro|A|', 'Conde|Antonio J|AJ|', 'Estevez|Roberto|R|', 'Valencia de Pablo|Oliver|O|', 'Rossi-Fedele|Giampiero|G|', 'Cisneros|Rafael|R|']
D000818:Animals D004357:Drug Synergism D006000:Glycocholic Acid D066298:In Vitro Techniques D009061:Mouth Mucosa D010159:Palate D012388:Root Canal Irrigants D012973:Sodium Hypochlorite D013501:Surface-Active Agents D013552:Swine
sodium hypochlorite glycocholic acid surfactant dissolution Dental pulp