Hemin offers neuroprotection through inducing exogenous neuroglobin in focal cerebral hypoxic-ischemia in rats.
OBJECTIVE
To investigate the inducible effect of hemin on exogenous neuroglobin (Ngb) in focal cerebral hypoxic-ischemia in rats.


METHODS
125 healthy SD rats were randomly divided into five groups: sham-operation control group, operation group, hemin treatment group, exogenous Ngb treatment group, and hemin and exogenous Ngb joint treatment group. Twenty-four hours after focal cerebral hypoxic-ischemia, Ngb expression was evaluated by immunocytochemistry, RT-PCR, and western blot analyses, while the brain water content and infarct volume were examined.


RESULTS
Immunocytochemistry, RT-PCR, and western blot analyses showed more pronounced Ngb expression in the hemin and exogenous Ngb joint operation group than in the hemin or exogenous Ngb individual treatment groups, thus producing significant differences in brain water content and infarct volume (p<0.05).


CONCLUSIONS
Hemin may be beneficial in protecting against focal cerebral hypoxic-ischemia through inducing the expression of exogenous Ngb.
['Song|Xue|X|', 'Xu|Rui|R|', 'Xie|Fei|F|', 'Zhu|Haiyuan|H|', 'Zhu|Ji|J|', 'Wang|Xin|X|']
D000818:Animals D001921:Brain D001929:Brain Edema D019610:Cytoprotection D004195:Disease Models, Animal D005786:Gene Expression Regulation D018014:Gene Transfer Techniques D015316:Genetic Therapy D005914:Globins D006427:Hemin D020925:Hypoxia-Ischemia, Brain D020244:Infarction, Middle Cerebral Artery D008297:Male D009419:Nerve Tissue Proteins D000078787:Neuroglobin D018696:Neuroprotective Agents D012333:RNA, Messenger D017207:Rats, Sprague-Dawley D013997:Time Factors
Hemin neuroprotection neuroglobin focal cerebral hypoxic-ischemia