The antioxidant activities effect of neutral and acidic polysaccharides from Epimedium acuminatum Franch. on Caenorhabditis elegans.
A neutral polysaccharide (EAP-1N) and an acidic polysaccharide (EAP-2A) were purified from Epimedium acuminatum by DEAE-52 cellulose anion-exchange chromatography and gel-filtration chromatography. Their structures were characterized by chemical composition analysis, high-performance size exclusion chromatography (HPSEC), Fourier transform infrared spectrometry (FT-IR), and gas chromatography-mass spectrometry (GC-MS). Further, their antioxidant activities were investigated both in vitro and in vivo. Results showed that EAP-2A had higher uronic acid content and larger average molecular weight than EAP-1N. Compared with EAP-1N, EAP-2A exhibited significantly scavenging activities against free radical in vitro, as well as strongly stimulating effect on antioxidant enzyme activities (including superoxide dismutases (SOD), catalases (CAT), and glutathione peroxidases (GSH-PX)) and preferably inhibitory effect on lipid peroxidation and protein carboxyl in the mode of Caenorhabditis elegans.
['Xu|Zhou|Z|', 'Feng|Shiling|S|', 'Shen|Shian|S|', 'Wang|Handong|H|', 'Yuan|Ming|M|', 'Liu|Jing|J|', 'Huang|Yan|Y|', 'Ding|Chunbang|C|']
D000818:Animals D017173:Caenorhabditis elegans D002374:Catalase D034181:Epimedium D016166:Free Radical Scavengers D005979:Glutathione Peroxidase D015227:Lipid Peroxidation D010269:Paraquat D011134:Polysaccharides D050050:Protein Carbonylation D017382:Reactive Oxygen Species D013482:Superoxide Dismutase
Antioxidant activities Acidic polysaccharide Epimedium acuminatum Franch Caenorhabditis elegans Neutral polysaccharide