A Light-Field Metasurface for High-Resolution Single-Particle Tracking.
Three-dimensional (3D) single-particle tracking (SPT) is a key tool for studying dynamic processes in the life sciences. However, conventional optical elements utilizing light fields impose an inherent trade-off between lateral and axial resolution, preventing SPT with high spatiotemporal resolution across an extended volume. We overcome the typical loss in spatial resolution that accompanies light-field-based approaches to obtain 3D information by placing a standard microscope coverslip patterned with a multifunctional, light-field metasurface on a specimen. This approach enables an otherwise unmodified microscope to gather 3D information at an enhanced spatial resolution. We demonstrate simultaneous tracking of multiple fluorescent particles within a large 0.5 × 0.5 × 0.3 mm3 volume using a standard epi-fluorescent microscope with submicron lateral and micron-level axial resolution.
['Holsteen|Aaron L|AL|', 'Lin|Dianmin|D|0000-0001-8343-9120', 'Kauvar|Isaac|I|', 'Wetzstein|Gordon|G|', 'Brongersma|Mark L|ML|0000-0003-1777-8970']
Light-field metasurface single-particle tracking Mie resonance interleaved metasurface microlens array point spread function engineering