New photostable naphthalimide-based fluorescent probe for mitochondrial imaging and tracking.
Monitoring mitochondria morphological changes temporally and spatially exhibits significant importance for diagnosing, preventing and treating various diseases related to mitochondrial dysfunction. However, the application of commercially available mitochondria trackers is limited due to their poor photostability. To overcome these disadvantages, we designed and synthesized a mitochondria-localized fluorescent probe by conjugating 1,8-naphthalimide with triphenylphosphonium (i.e. NPA-TPP). The structure and characteristic of NPA-TPP was characterized by UV-vis, fluorescence spectroscopy, (1)HNMR, (13)CNMR, FTIR, MS, etc. The photostability and cell imaging were performed on the laser scanning confocal microscopy. Moreover, the cytotoxicity of NPA-TPP on cells was evaluated using (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The results showed that NPA-TPP not only has high sensitivity and specificity to mitochondria, but also exhibits super-high photostability, negligible cytotoxicity and good water solubility. In short, NPA-TPP indicates great potential for targeting mitochondria and enables a real-time and long-term tracking mitochondrial dynamics changes.
['Huang|Saipeng|S|', 'Han|Rongcheng|R|', 'Zhuang|Qianfen|Q|', 'Du|Libo|L|', 'Jia|Hongying|H|', 'Liu|Yangping|Y|', 'Liu|Yang|Y|']
D058948:Cell Tracking D003287:Contrast Media D004355:Drug Stability D005456:Fluorescent Dyes D006801:Humans D008027:Light D061986:MCF-7 Cells D008856:Microscopy, Fluorescence D008928:Mitochondria D053644:Naphthalimides
Photostability Fluorescent probe Mitochondrial imaging 1,8-naphthalimides Real-time tracking