Biophysical characterization and structural determination of the potent cytotoxic Psathyrella asperospora lectin.
A lectin with strong cytotoxic effect on human colon cancer HT29 and monkey kidney VERO cells was recently identified from the Australian indigenous mushroom Psathyrella asperospora and named PAL. We herein present its biochemical and structural analysis using a multidisciplinary approach. Glycan arrays revealed binding preference towards N-acetylglucosamine (GlcNAc) and, to a lesser extent, towards sialic acid (Neu5Ac). Submicromolar and millimolar affinity was measured by surface plasmon resonance for GlcNAc and NeuAc, respectively. The structure of PAL was resolved by X-ray crystallography, elucidating both the protein's amino acid sequence as well as the molecular basis rationalizing its binding specificity. Proteins 2017; 85:969-975. © 2016 Wiley Periodicals, Inc.
['Ribeiro|João P|JP|', 'Ali Abol Hassan|Mohamed|M|', 'Rouf|Razina|R|', 'Tiralongo|Evelin|E|', 'May|Tom W|TW|', 'Day|Christopher J|CJ|', 'Imberty|Anne|A|', 'Tiralongo|Joe|J|', 'Varrot|Annabelle|A|']
D000117:Acetylglucosamine D000363:Agaricales D000818:Animals D000970:Antineoplastic Agents D001665:Binding Sites D002232:Carbocyanines D002522:Chlorocebus aethiops D018360:Crystallography, X-Ray D005456:Fluorescent Dyes D048690:Fruiting Bodies, Fungal D005656:Fungal Proteins D019073:HT29 Cells D006801:Humans D037102:Lectins D046228:Microarray Analysis D019158:N-Acetylneuraminic Acid D011485:Protein Binding D000072756:Protein Conformation, alpha-Helical D000072757:Protein Conformation, beta-Strand D054730:Protein Interaction Domains and Motifs D013194:Staining and Labeling D020349:Surface Plasmon Resonance D014709:Vero Cells
GlcNAc recognition beta-propeller fungal lectins