Tailoring the supramolecular structure of aminated polyrotaxanes toward enhanced cellular internalization.
The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated by flow cytometry and confocal laser scanning microscopy. AF-545-labeled aminated PRXs (APRXs) containing different numbers of threaded α-cyclodextrins (CDs) and amino groups are synthesized; their cellular uptakes are analyzed using HeLa cells in serum. The APRX threaded CD number is discovered to be a more critical factor for enhancing cellular internalization than the APRX amine content. Additionally, APRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules. Because increased numbers of threaded CDs are related to increased PRX rigidity, the PRX rigid frame resulting from CD molecules threaded on a poly(ethylene glycol) (PEG) chain is suitable for intracellular tools in therapy and diagnosis.
['Yokoyama|Nanako|N|', 'Seo|Ji-Hun|JH|', 'Tamura|Atsushi|A|', 'Sasaki|Yoshihiro|Y|', 'Yui|Nobuhiko|N|']
D000586:Amination D001692:Biological Transport D002470:Cell Survival D003505:Cyclodextrins D004337:Drug Carriers D005456:Fluorescent Dyes D006367:HeLa Cells D006801:Humans D015394:Molecular Structure D020442:Poloxamer D011092:Polyethylene Glycols D043862:Rotaxanes D047391:alpha-Cyclodextrins
supramolecular structures polyrotaxane cellular internalization cyclodextrin electrostatic interaction