Fusarium infection causes genotoxic disorders and antioxidant-based damages in Orobanche spp.
This study aims to evaluate the toxic effects of Fusarium oxysporum on root parasitic weed, Orobanche spp. Comparative genetic and gene expression studies were conducted on uninfected and fungus-infected orobanches. In genetic studies, isolated total DNA was amplified by RAPD PCR. Fragment properties were analysed by GTS test. According to the results, the fragment properties of control and Fusarium infected (experimental) groups varied widely; and it has been observed that Fusarium has genotoxic effects on the DNA of orobanches. In gene expression studies, the expression levels of genes encoding enzymes or proteins were associated with ROS damage and toxic effects, therefore, gene expressions of Mn-superoxide dismutase (SOD), Zn-superoxide dismutase (=SOD2, mitochondrial), glutamine synthetase (GS), heat shock protein gene (HSP70), BAX, Caspase-3 and BCL2 were significantly higher in the experimental group. In the light of obtained data, it was concluded that F. oxysporum (1) caused heavy ROS damage in Orobanche (2) induced significant irrevocable genotoxic effects on the DNA of Orobanche, (3) degraded protein metabolism and synthesis, and finally (4) triggered apoptosis. The results of this study can be a ground for further research on reducing the toxic effects of Fusarium on agricultural products, so that advancements in bio-herbicide technology may provide a sustainable agricultural production.
['Aybeke|Mehmet|M|']
D000975:Antioxidants D061046:Biological Control Agents D053148:Caspase 3 D018744:DNA, Plant D060585:Fusariosis D005670:Fusarium D018506:Gene Expression Regulation, Plant D018745:Genome, Plant D005974:Glutamate-Ammonia Ligase D006360:Heat-Shock Proteins D006540:Herbicides D008928:Mitochondria D009152:Mutagenicity Tests D009183:Mycotoxins D031672:Orobanche D010935:Plant Diseases D018517:Plant Roots D019253:Proto-Oncogene Proteins c-bcl-2 D019105:Random Amplified Polymorphic DNA Technique D013482:Superoxide Dismutase D051028:bcl-2-Associated X Protein
Biocontrol Broomrape Mycoherbicide Parasitic plant