Cellular signaling circuits interfaced with synthetic, post-translational, negating Boolean logic devices.
A negating functionality is fundamental to information processing of logic circuits within cells and computers. Aiming to adapt unutilized electronic concepts to the interrogation of signaling circuits in cells, we first took a bottom-up strategy whereby we created protein-based devices that perform negating Boolean logic operations such as NOT, NOR, NAND, and N-IMPLY. These devices function in living cells within a minute by precisely commanding the localization of an activator molecule among three subcellular spaces. We networked these synthetic gates to an endogenous signaling circuit and devised a physiological output. In search of logic functions in signal transduction, we next took a top-down approach and computationally screened 108 signaling pathways to identify commonalities and differences between these biological pathways and electronic circuits. This combination of synthetic and systems approaches will guide us in developing foundations for deconstruction of intricate cell signaling, as well as construction of biomolecular computers.
['Razavi|Shiva|S|', 'Su|Steven|S|', 'Inoue|Takanari|T|']
D000465:Algorithms D000818:Animals D000900:Anti-Bacterial Agents D019556:COS Cells D002522:Chlorocebus aethiops D008164:Luminescent Proteins D018613:Microscopy, Confocal D008928:Mitochondria D008962:Models, Theoretical D055503:Protein Multimerization D011499:Protein Processing, Post-Translational D015398:Signal Transduction D020123:Sirolimus D022021:Tacrolimus Binding Proteins D014162:Transfection
negation signaling pathway Rac chemically induced dimerization membrane ruffling synthetic Boolean logic