Plasmonic laser treatment for Morpholino oligomer delivery in antisense applications.
Several cell transfection techniques have been developed in the last decades for specific applications and for various types of molecules. In this context, laser based approaches are of great interest due to their minimal invasiveness and spatial selectivity. In particular, laser induced plasmon based delivery of exogenous molecules into cells can have great impact on future applications. This approach allows high-throughput laser transfection by excitation of plasmon resonances at gold nanoparticles non-specifically attached to the cell membrane. In this study, we demonstrate specific gene-knockdown by transfection of Morpholino oligos using this technique with optimized particle size. Furthermore, we evaluated the cytotoxicity of plasmonic laser treatment by various assays, including LDH activity and ROS formation. In summary, this study gives important insights into this new approach and clearly demonstrates its relevance for possible biological applications.
['Kalies|Stefan|S|', 'Heinemann|Dag|D|', 'Schomaker|Markus|M|', 'Murua Escobar|Hugo|H|', 'Heisterkamp|Alexander|A|', 'Ripken|Tammo|T|', 'Meyer|Heiko|H|']
D000818:Animals D015153:Blotting, Western D045744:Cell Line, Tumor D002462:Cell Membrane D002470:Cell Survival D003911:Dextrans D004285:Dogs D005110:Extracellular Space D055785:Gene Knockdown Techniques D017612:Gold Compounds D049452:Green Fluorescent Proteins D006861:Hydrogen Peroxide D007770:L-Lactate Dehydrogenase D007834:Lasers D053768:Metal Nanoparticles D060172:Morpholinos D010316:Particle Size D017382:Reactive Oxygen Species D014162:Transfection
plasmon Morpholino laser transfection gold nanoparticle knockdown