MiR-362 suppresses cervical cancer progression via directly targeting BAP31 and activating TGFβ/Smad pathway.
BAP31 (B-cell receptor-associated protein 31) is an important regulator of intracellular signal transduction and highly expressed in several cancer tissues or testicular tissues. Our previous study had revealed that elevated BAP31 plays a crucial role in the progress and metastasis of cervical cancer. Even so, the precise mechanism of abnormal BAP31 elevation in cervical cancer has not been fully elucidated. We revealed that the expression of BAP31 was mainly regulated by microRNA-362 (miR-362), which was markedly downregulated in cervical cancer tissues and negatively correlated with clinical tumor staging. Overexpression of miR-362 inhibited cervical cancer cell proliferation and increased the proportion of apoptotic cells. Furthermore, miR-362 reduced the tumor sizes and prolonged mice survival time in xenograft nude mice model. Finally, we demonstrated that the BAP31/SPTBN1 complex regulated tumor progression through the Smad 2/3 pathway under the control of miR-362. Collectively, our findings demonstrated that miR-362 could work as an anti-oncomiR that inhibits proliferation and promotes apoptosis in cervical cancer cells via BAP31 and TGFβ/Smad pathway. Overexpression of miR-362 might be a potential therapeutic strategy for cervical cancer.
['Yang|Shuya|S|', 'Sun|Yuanjie|Y|', 'Jiang|Dongbo|D|', 'Wang|Jing|J|', 'Dang|Erle|E|', 'Li|Zichao|Z|', 'Zhou|Jiayi|J|', 'Lu|Yuchen|Y|', 'Shi|Jingqi|J|', 'Tao|Liang|L|', 'Wang|Jun|J|', 'Jin|Boquan|B|', 'Zheng|Lianhe|L|', 'Yang|Kun|K|0000-0003-0305-6256']
D000818:Animals D017209:Apoptosis D049109:Cell Proliferation D018450:Disease Progression D005260:Female D015972:Gene Expression Regulation, Neoplastic D006367:HeLa Cells D006801:Humans D008565:Membrane Proteins D008819:Mice, Nude D035683:MicroRNAs D015398:Signal Transduction D051899:Smad2 Protein D051900:Smad3 Protein D016212:Transforming Growth Factor beta D047368:Tumor Burden D002583:Uterine Cervical Neoplasms
miR-362 cervical cancer BAP31 TGFβ/Smad pathway anti-oncomiR