124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics.
Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes.
['Wade|Orsolya K|OK|', 'Woehrstein|Johannes B|JB|', 'Nickels|Philipp C|PC|', 'Strauss|Sebastian|S|', 'Stehr|Florian|F|', 'Stein|Johannes|J|0000-0002-1335-1120', 'Schueder|Florian|F|', 'Strauss|Maximilian T|MT|', 'Ganji|Mahipal|M|', 'Schnitzbauer|Joerg|J|', 'Grabmayr|Heinrich|H|', 'Yin|Peng|P|0000-0002-2769-6357', 'Schwille|Petra|P|0000-0002-6106-4847', 'Jungmann|Ralf|R|0000-0003-4607-3312']
D003198:Computer Simulation D004247:DNA D007700:Kinetics D008856:Microscopy, Fluorescence D009690:Nucleic Acid Conformation D009841:Oligonucleotides D011506:Proteins D012313:RNA
DNA-PAINT multiplexing Super-resolution microscopy barcoding DNA nanotechnology