Effects of hydroxyapatite-coated nonwoven polyethylene/polypropylene fabric on non-mesodermal lineage-specific differentiation of human adipose-derived stem cells.
OBJECTIVE
Compared to other stem cells, the multipotency of human adipose-derived mesenchymal stem cells (ASCs) is limited. Effective approaches that trigger or enhance lineage-specific transdifferentiation are highly envisaged in the improvement of ASCs-based cell therapies. Using Immunofluorescence assays and the secretion of cardiac troponin T (cTnT) protein, we studied the impact of two substrates: Hydroxyapatite (HAp)-coated nonwoven polyethylene (PET)/polypropylene (PP) fabric and glass surfaces, representing 3 dimensional (D) and 2 D environments respectively, on the induction of cardiomyocytes - a non-mesodermal cell type from ASCs for 1-5 weeks.


RESULTS
ASCs were successfully isolated from human adipose tissue under cGMP conditions. Within 1-3 weeks, expression of cTnT in the induced 3D cultures was overall significantly higher (P < 0.021) than that in the induced 2D cultures or controls (P < 0.0009). Remarkably, after 3 weeks of culture, cTnT secretion in the induced 3D cultures gradually declined, nearly reaching levels observed in the 2D cultures. The results show that HAp-coated nonwoven PE/PP fabric could enhance lineage-specific differentiation of ASCs toward cardiac-like cells. However, the fabric might suppress growth of the transformed cells. These preliminary findings encourage further interest in validating the fabric's potential in improving ASCs transdifferentiation.
['Ntege|Edward Hosea|EH|', 'Sunami|Hiroshi|H|', 'Denda|Junko|J|', 'Futenma|Naoko|N|', 'Shimizu|Yusuke|Y|http://orcid.org/0000-0002-2528-2003']
D000273:Adipose Tissue D002454:Cell Differentiation D002478:Cells, Cultured D017886:Durapatite D006801:Humans D020959:Polyethylene D011126:Polypropylenes D013234:Stem Cells
Mesenchymal stem cells Transdifferentiation Cell therapy Hydroxyapatite Cardiomyocytes Adipose tissue-derived stem cells Nonwoven scaffold