CPSF7 regulates liver cancer growth and metastasis by facilitating WWP2-FL and targeting the WWP2/PTEN/AKT signaling pathway.
Alternative splicing within a gene can create different versions of an mRNA, called isoforms. CFIm, composed of a small subunit CFIm25 and two large subunits CFIm68 and CFIm59 (also known as CPSF7), has been proposed as an enhancer-dependent activator of mRNA 3' processing. In this study, we investigated the role of CPSF7 in hepatocellular carcinoma. Experimental evidence suggests that the expression level of CPSF7 is higher in liver cancer cells and tissues than in non-tumor hepatic cells and tissues. Furthermore, knockdown of CPSF7 effectively suppressed cell proliferation, migration and colony formation in liver cancer cells by inhibiting PTEN/AKT signaling. CPSF7 promoted WWP2-FL due to the presence of PTEN ubiquitination sites in this longer transcript. Taken together, we identified that CPSF7 regulates liver cancer growth by targeting WWP2-FL that in turn regulates AKT activation in a PTEN-dependent manner.
['Fang|Shiji|S|', 'Zhang|Dengke|D|', 'Weng|Wei|W|', 'Lv|Xiuling|X|', 'Zheng|Liyun|L|', 'Chen|Minjiang|M|', 'Fan|Xiaoxi|X|', 'Mao|Jianting|J|', 'Mao|Chenchen|C|', 'Ye|Yani|Y|', 'Xu|Min|M|', 'Ji|Jiansong|J|']
D000818:Animals D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D005260:Female D006801:Humans D008113:Liver Neoplasms D008297:Male D051379:Mice D008819:Mice, Nude D008875:Middle Aged D051059:PTEN Phosphohydrolase D020033:Protein Isoforms D051057:Proto-Oncogene Proteins c-akt D034622:RNA Interference D000067776:RNA Recognition Motif Proteins D034741:RNA, Small Interfering D015398:Signal Transduction D014183:Transplantation, Heterologous D044767:Ubiquitin-Protein Ligases D039221:mRNA Cleavage and Polyadenylation Factors
CPSF7 WWP2 PTEN/AKT signaling Hepatocellular carcinoma Alternative polyadenylation