Pinocembrin Ameliorates Cognitive Impairment Induced by Vascular Dementia: Contribution of Reelin-dab1 Signaling Pathway.
Background
As a substrate of apoER2, Reelin has been verified to exert neuroprotection by preventing memory impairment. Pinocembrin is the most abundant natural flavonoid found in propolis, and it has been used to exert neuroprotection, blood-brain barrier protection, anti-oxidation, and inflammation diminishing, both in vitro and in vivo. However, the roles and molecular mechanisms of pinocembrin in neurobehavioral outcomes and neuronal repair after vascular dementia are still under investigation.


Purpose
To explore the role of pinocembrin in the involvement of the Reelin-dab1 signaling pathway in improving memory impairment, both in cell culture and animals experiments.


Material and Methods
Behavioral tests were conducted on day 48 to confirm the protection of pinocembrin against cognitive impairment. Cell and molecular biology experiments demonstrated that the Reelin-dab1 pathway mediates the underlying mechanism of cognitive improvement by pinocembrin.


Results
It was showed that pinocembrin alleviated learning and memory deficits induced by vascular dementia, by inducing the expression of Reelin, apoER2, and p-dab1 in the hippocampus. The expression of Reelin and p-dab1 was both inhibited following Reelin RNA interference in SH-SY5Y prior to oxygen glucose deprivation (OGD) injury, suggesting that Reelin played a core role in pinocembrin's effect on OGD in vitro.


Conclusion
Pinocembrin improves the cognition via the Reelin-dab1 signaling pathway.
['Kang|Ze-Chun|ZC|', 'Wang|Hai-Gang|HG|', 'Yang|Yu-Lin|YL|', 'Zhao|Xiao-Yue|XY|', 'Zhou|Qi-Meng|QM|', 'Yang|Ying-Lin|YL|', 'Yang|Jing-Yu|JY|', 'Du|Guan-Hua|GH|']
D048868:Adaptor Proteins, Signal Transducing D000818:Animals D001522:Behavior, Animal D015816:Cell Adhesion Molecules, Neuronal D045744:Cell Line, Tumor D002470:Cell Survival D002478:Cells, Cultured D060825:Cognitive Dysfunction D015140:Dementia, Vascular D004305:Dose-Response Relationship, Drug D016326:Extracellular Matrix Proteins D044950:Flavanones D006801:Humans D008297:Male D009419:Nerve Tissue Proteins D034741:RNA, Small Interfering D051381:Rats D017208:Rats, Wistar D000091042:Reelin Protein D012697:Serine Endopeptidases D015398:Signal Transduction
pinocembrin cognitive impairment vascular dementia signaling pathway Reelin hippocampus