Toxicology of long-term and high-dose administration of methylphenidate on the kidney tissue - a histopathology and molecular study.
The present study aims to assess the influences of oral methylphenidate on kidney function and structure versus vehicle treatment in adult male rats. In this study, thirty adult male rats equally into two treatment groups divided randomly, and among them, MPH has been administered for 21 days, at doses of 20 mg/kg, and the control group has received salin. In renal, under the effect of MPH applying quantitative real-time PCR, we analyzed nephrotoxicity-related molecular pathways like autophagy, inflammation, and apoptosis. Moreover, the levels of GSH, CAT, and SOD were investigated as antioxidant enzymes. Afterward, stereological analysis in MPH-treated rats has been performed. Analysis of qPCR displayed inflammation, impaired autophagy, and enhanced apoptosis with histological changes in the kidney's tissue, also an important rise in the antioxidant enzymes' level. Besides, 20 mg/kg of MPH led to a decline in the mean of Bowman's space thickness and renal corpuscle's volume in comparison to the control rats. Collectively, our histological and molecular data implicit that in the kidney region, administrating of MPH evoked discriminative expression alterations in nephrotoxicity-associated signaling cascades, specifically autophagy, inflammation, and apoptosis paired with important damage to kidney tissue.
['Raoofi|Amir|A|', 'Delbari|Ali|A|', 'Mahdian|Davood|D|', 'Mojadadi|Mohammad-Shafi|MS|', 'Amini|Abdollah|A|', 'Javadinia|Sara Sadat|SS|', 'Dadashizadeh|Ghazaleh|G|', 'Ahrabi|Behnaz|B|', 'Ebrahimi|Vahid|V|', 'Mousavi Khaneghah|Amin|A|https://orcid.org/0000-0001-5769-0004']
D000284:Administration, Oral D000818:Animals D017209:Apoptosis D001343:Autophagy D000697:Central Nervous System Stimulants D004305:Dose-Response Relationship, Drug D005786:Gene Expression Regulation D007668:Kidney D007674:Kidney Diseases D008297:Male D008774:Methylphenidate D018384:Oxidative Stress D017208:Rats, Wistar D015398:Signal Transduction D013997:Time Factors
methylphenidate kidney Autophagy inflammation apoptosis nephrotoxicity