Enzyme-free Target Recycling and Double-Output Amplification System for Electrochemiluminescent Assay of Mucin 1 with MoS2 Nanoflowers as Co-reaction Accelerator.
In this work, a sensitive electrochemiluminescent assay of mucin 1 (MUC1) was developed with the advantages of target recycling amplification strategy and effective MoS2 nanoflower (MoS2 NF)-based signal probe. Briefly, the target MUC1 triggered enzyme-free recycling and a double-output amplification process was executed to acquire masses of single-stranded DNA as a mimic target, which further participated in the catalytic hairpin assembly process for signal amplification. Meanwhile, MoS2 NFs were prepared as an effective co-reaction accelerator, which not only possessed excellent catalytic performance for H2O2 decomposition to largely enhance the luminous intensity of N-(aminobutyl)- N-(ethylisoluminol) (ABEI)-H2O2 electrochemiluminescence system but also offered a desirable platform for ABEI-functionalized Ag nanoparticles (ABEI-Ag complexes) loading via Ag-S binding. The experimental results showed the proposed aptasensor had a good linear relationship in the range of 1 fg/mL to 10 ng/mL for MUC1 detection and the limit of detection was 0.58 fg/mL (S/N = 3). In addition, the aptasensor had nice stability and selectivity and huge potential to be applied in clinical research.
['Li|Sheng-Kai|SK|', 'Liu|Zhi-Ting|ZT|', 'Li|Ji-Yang|JY|', 'Chen|An-Yi|AY|', 'Chai|Ya-Qin|YQ|', 'Yuan|Ruo|R|http://orcid.org/0000-0003-3664-6236', 'Zhuo|Ying|Y|http://orcid.org/0000-0002-4491-1186']
D052157:Aptamers, Nucleotide D015374:Biosensing Techniques D004220:Disulfides D055664:Electrochemical Techniques D006046:Gold D006861:Hydrogen Peroxide D008163:Luminescent Measurements D053768:Metal Nanoparticles D008982:Molybdenum D018396:Mucin-1 D049329:Nanostructures
double-output amplification electrochemiluminescence MoS2 nanoflowers co-reaction accelerator aptasensor