Laccase immobilized polyaniline/magnetic graphene composite electrode for detecting hydroquinone.
Magnetic graphene nanocomposites were prepared by hydrothermal synthesis, and aniline polymerization was initiated by magnetic graphene. These polyaniline/magnetic graphene (PANI/MG) composites were used to immobilize laccase to construct biosensors. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and infrared spectroscopy (IR) were used to characterize these composites. Cyclic voltammetry and chronoamperometry technique were used to test the electrical properties of the constructed polyaniline/magnetic graphene laccase modified electrode. The results show that the polyaniline/magnetic graphene immobilizing laccase modified electrode exhibited superior electrical properties, including high sensitivity, detection limit and linear range. The hydroquinone was used as an analytical and detection probe. The selectivity was 0.03639 A/(mol/L), the linear range was 0.4-337.2 μmol/L, and the detection limit was 2.94 μM (signal/noise = 3, minimum identification value of effective signal). The biosensor can reach the conditions for detecting the actual water sample.
['Lou|Congqiang|C|', 'Jing|Tao|T|', 'Zhou|Juying|J|', 'Tian|Jingzhi|J|', 'Zheng|Yongjie|Y|', 'Wang|Chao|C|', 'Zhao|Zhiyuan|Z|', 'Lin|Jing|J|', 'Liu|Hu|H|', 'Zhao|Chunqi|C|', 'Guo|Zhanhu|Z|']
D000814:Aniline Compounds D015374:Biosensing Techniques D002244:Carbon D002384:Catalysis D004563:Electrochemistry D004566:Electrodes D004800:Enzymes, Immobilized D005898:Glass D006108:Graphite D006873:Hydroquinones D042845:Laccase D060328:Magnetic Phenomena D017550:Spectroscopy, Fourier Transform Infrared D014867:Water
Laccase Graphene Sensor