17β-Hydroxysteroid dehydrogenase type 10 predicts survival of patients with colorectal cancer and affects mitochondrial DNA content.
Mitochondrial energy production is reduced in tumor cells, and altered mitochondrial respiration contributes to tumor progression. Synthesis of proteins coded by mitochondrial DNA (mtDNA) requires the correct processing of long polycistronic precursor RNA molecules. Mitochondrial RNase P, composed of three different proteins (MRPP1, HSD10, and MRPP3), is necessary for correct RNA processing. Here we analyzed the role of RNase P proteins in colorectal cancer. High HSD10 expression was found in 28%; high MRPP1 expression in 40% of colorectal cancers, respectively. Expression of both proteins was not significantly associated with clinicopathological parameters. Survival analysis revealed that loss of HSD10 expression is associated with poor prognosis. Cox regression demonstrated that patients with high HSD10 tumors are at lower risk. High HSD10 expression was significantly associated with high mtDNA content in tumor tissue. A causal effect of HSD10 overexpression or knock down with increased or reduced mtDNA levels, respectively, was confirmed in tumor cell lines. Our data suggest that HSD10 plays a role in alterations of energy metabolism by regulating mtDNA content in colorectal carcinomas, and HSD10 protein analysis may be of prognostic value.
['Amberger|Albert|A|', 'Deutschmann|Andrea J|AJ|', 'Traunfellner|Pia|P|', 'Moser|Patrizia|P|', 'Feichtinger|René G|RG|', 'Kofler|Barbara|B|', 'Zschocke|Johannes|J|']
D015094:3-Hydroxyacyl CoA Dehydrogenases D000368:Aged D045744:Cell Line, Tumor D015179:Colorectal Neoplasms D004272:DNA, Mitochondrial D005260:Female D006801:Humans D008297:Male D008875:Middle Aged D011379:Prognosis
17β-Hydroxysteroid dehydrogenase type 10 Survival Colorectal cancer Mitochondrial DNA Prognostic marker