Development of a novel rhesus macaque model with an infectious R5 simian-human immunodeficiency virus encoding HIV-1 CRF08_BC env.
BACKGROUND
The CRF08_BC strain is one of the most predominant circulating Human immunodeficiency virus type 1 (HIV-1) strains in the Chinese pandemic. A simian-human immunodeficiency virus (SHIV) encoding HIV-1 CRF08_BC env is highly desirable to evaluate candidate AIDS vaccines in non-human primates.


METHODS
SHIV-KBQJ-12, which carries the envelope glycoprotein from QJ001, an infectious molecular clone of HIV-1 CRF08_BC, was generated. The replication capacity of SHIV-KBQJ-12 was determined both in human and rhesus macaque (Macaca mulatta) peripheral blood mononuclear cells (PBMCs) and in Chinese rhesus macaques.


RESULTS
SHIV-KBQJ-12 replicated efficiently in human and macaque PBMCs and displayed a preference for CCR5 as an entry coreceptor. Productive infection of two macaques by intravenous inoculation with SHIV-KBQJ-12 was confirmed.


CONCLUSIONS
SHIV-KBQJ-12 is an R5-tropic chimeric virus that can establish productive infection both in vitro and in vivo in Chinese rhesus macaques and will be useful to assess candidate HIV-1 CRF08_BC vaccines in China.
['Huang|Li|L|', 'Kusagawa|Shigeru|S|', 'Zeng|Haiyan|H|', 'Yang|Guibo|G|', 'Sun|Binlian|B|', 'Miura|Tomoyuki|T|', 'Yang|Rongge|R|']
D016915:AIDS Vaccines D000818:Animals D018791:CD4 Lymphocyte Count D002681:China D004195:Disease Models, Animal D005260:Female D005434:Flow Cytometry D016679:Genome, Viral D015658:HIV Infections D015497:HIV-1 D006801:Humans D007963:Leukocytes, Mononuclear D008253:Macaca mulatta D008297:Male D008969:Molecular Sequence Data D010802:Phylogeny D019713:Receptors, CCR5 D020133:Reverse Transcriptase Polymerase Chain Reaction D017422:Sequence Analysis, DNA D015302:Simian Immunodeficiency Virus D014162:Transfection D019562:Viral Load D014779:Virus Replication D054299:env Gene Products, Human Immunodeficiency Virus
R5 simian-human immunodeficiency virus CRF08_BC peripheral blood mononuclear cell Chinese rhesus macaques