Preparation of glutaraldehyde-treated lipase-inorganic hybrid nanoflowers and their catalytic performance as immobilized enzymes.
The use of protein-inorganic hybrid nanoflowers for the immobilization of enzymes has received a significant degree of attention owing to their capability to retain high enzymatic activity and stability. However, the relative lack of reusability due to the weakness of the flower-like structure has limited their practical applications. Herein, we have developed a simple but efficient method to synthesize highly robust enzyme-inorganic hybrid nanoflowers, which relies on further crosslinking of the enzyme molecules entrapped in the hybrid nanoflowers by treatment with glutaraldehyde (GA). By employing lipase from Candida rugosa as a model enzyme with copper phosphate during 3days incubation followed by the additional GA treatment for only 1h, we could successfully synthesize GA-treated lipase nanoflowers having similar flower-like morphology and hydrolytic activity (ca. 95% compared with the free lipase) as conventionally synthesized lipase nanoflowers without GA treatment. Importantly, the conventional lipase nanoflowers seemed not to be reusable because they lost most of their activity (∼90%) after recycling 4 times, whereas GA-treated lipase nanoflowers exhibited higher retention of their initial activity (over 70%) after 4 reuses, which was also accompanied by an efficient maintenance of their flower-like morphology. Based on our results, we expect that this simple GA-mediated strategy to synthesize enzyme-inorganic hybrid nanoflowers can be readily extended to other enzymes for various biotechnological applications.
['Lee|Hye Rin|HR|', 'Chung|Minsoo|M|', 'Kim|Moon Il|MI|', 'Ha|Sung Ho|SH|']
D055162:Biocatalysis D001709:Biotechnology D002175:Candida D003432:Cross-Linking Reagents D004800:Enzymes, Immobilized D005656:Fungal Proteins D005976:Glutaral D008049:Lipase D008855:Microscopy, Electron, Scanning D046529:Microscopy, Electron, Transmission D049329:Nanostructures D036103:Nanotechnology
Protein-inorganic hybrid nanoflowers Reusability Candida rugosa lipase Glutaraldehyde treatment