Efficacy of chitinase-3-like protein 1 as an in vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells.
Introduction
Maxillary/mandibular bone marrow stromal cells (MBMSCs) are a useful cell source for bone regeneration in the oral and maxillofacial region. To further ensure the clinical application of MBMSCs in bone regenerative therapy, it is important to determine the bone formation capacity of MBMSCs before transplantation. The aim of this study is to identify the molecular marker that determines the in vivo bone formation capacity of MBMSCs.


Methods
The cell growth, cell surface antigens, in vitro and in vivo bone formation capacity of MBMSCs were examined. The amount of chitinase-3-like protein 1 (CHI3L1) secreted into the conditioned medium was quantified. The effects of CHI3L1 on the cell growth and osteogenic differentiation potential of MBMSCs and on the cell growth and migration of vascular endothelial cells and fibroblasts were examined.


Results
The cell growth, and in vitro and in vivo bone formation capacity of the cells treated with different conditions were observed. MBMSCs that secreted a large amount of CHI3L1 into the conditioned medium tended to have low in vivo bone formation capacity, whereas MBMSCs that secreted a small amount of CHI3L1 had greater in vivo bone formation capacity. CHI3L1 promoted the migration of vascular endothelial cells, and the cell growth and migration of fibroblasts.


Conclusion
Our study indicates that the in vitro osteogenic differentiation capacity of MBMSCs and the in vivo bone formation capacities of MBMSCs were not necessarily correlated. The transplantation of high CHI3L1 secretory MBMSCs may suppress bone formation by inducing fibrosis at the site. These results suggest that the CHI3L1 secretion levels from MBMSCs may be used as a predictable marker of bone formation capacity in vivo.
['Komabashiri|Naohiro|N|', 'Suehiro|Fumio|F|', 'Ishii|Masakazu|M|', 'Nishimura|Masahiro|M|']
Chitinase-3-like protein 1 Bone formation capacity Migration ALP, Alkaline phosphatase BMSC, bone marrow-derived stem cell CHI3L1, chitinase-3-like protein 1 FBS, fetal bovine serum HUVEC, human umbilical vein endothelial cells Jaw bone marrow stromal cells MBMSC, maxillary/mandibular bone marrow stromal cells MSCs, mesenchymal stem cells NHDF, normal human dermal fibroblasts α-MEM, alpha modified Eagle's minimum essential medium β-TCP, beta-tricalcium phosphate