Defective repair capacity of variant proteins of the DNA glycosylase NTHL1 for 5-hydroxyuracil, an oxidation product of cytosine.
The NTHL1 gene encodes DNA glycosylase, which is involved in base excision repair, and biallelic mutations of this gene result in NTHL1-associated polyposis (NAP), a hereditary disease characterized by colorectal polyposis and multiple types of carcinomas. However, no proper functional characterization of variant NTHL1 proteins has been done so far. Herein, we report functional evaluation of variant NTHL1 proteins to aid in the accurate diagnosis of NAP. First, we investigated whether it would be appropriate to use 5-hydroxyuracil (5OHU), an oxidation product of cytosine, for the evaluation. In the supF forward mutation assay, 5OHU caused an increase of the mutation frequency in human cells, and the C→T mutation was predominant among the 5OHU-induced mutations. In addition, in DNA cleavage activity assay, 5OHU was excised by NTHL1 as well as four other DNA glycosylases (SMUG1, NEIL1, TDG, and UNG2). When human cells overexpressing the five DNA glycosylases were established, it was found that each of the five DNA glycosylases, including NTHL1, had the ability to suppress 5OHU-induced mutations. Based on the above results, we performed functional evaluation of eight NTHL1 variants using 5OHU-containing DNA substrate or shuttle plasmid. The DNA cleavage activity assay showed that the variants of NTHL1, Q90X, Y130X, R153X, and Q287X, but not R19Q, V179I, V217F, or G286S, showed defective repair activity for 5OHU and two other oxidatively damaged bases. Moreover, the supF forward mutation assay showed that the four truncated-type NTHL1 variants showed a reduced ability to suppress 5OHU-induced mutations in human cells. These results suggest that the NTHL1 variants Q90X, Y130X, R153X, and Q287X, but not R19Q, V179I, V217F, or G286S, were defective in 5OHU repair and the alleles encoding them were considered to be pathogenic for NAP.
['Shinmura|Kazuya|K|', 'Kato|Hisami|H|', 'Kawanishi|Yuichi|Y|', 'Goto|Masanori|M|', 'Tao|Hong|H|', 'Yoshimura|Katsuhiro|K|', 'Nakamura|Satoki|S|', 'Misawa|Kiyoshi|K|', 'Sugimura|Haruhiko|H|']
D011125:Adenomatous Polyposis Coli D000483:Alleles D045744:Cell Line, Tumor D015179:Colorectal Neoplasms D053837:DNA Cleavage D045647:DNA Glycosylases D004252:DNA Mutational Analysis D004260:DNA Repair D043224:Deoxyribonuclease (Pyrimidine Dimer) D004847:Epithelial Cells D015870:Gene Expression D006801:Humans D009154:Mutation D014498:Uracil D051981:Uracil-DNA Glycosidase
DNA glycosylase NTHL1 5-hydroxyuracil NTHL1-associated polyposis supF forward mutation assay C→T mutation DNA cleavage activity assay