Structural characterization of phenolic compounds and antioxidant activity of the phenolic-rich fraction from defatted adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seed meal.
The current study aims to investigate the antioxidant activities of various extracts from defatted adlay seed meal (DASM) based on the oxygen radical absorbance capacity (ORAC) assay, peroxyl radical scavenging capacity (PSC) assay and cellular antioxidant activity (CAA) assay. Of all the fractions, the n-butanol fraction exhibited the highest antioxidant activity, followed by crude acetone extract and aqueous fractions. Of the three sub-fractions obtained by Sephadex LH-20 chromatography, sub-fraction 3 possessed the highest antioxidant activity and total phenolic content. There was a strong positive correlation between the total phenolic content and the antioxidant activity. Based on HPLC-DAD-ESI-MS/MS analysis, the most abundant phenolic acid in sub-fraction 3 of DASM was ferulic acid at 67.28 mg/g, whereas the predominant flavonoid was rutin at 41.11 mg/g. Of the major individual compounds in sub-fraction 3, p-coumaric acid exhibited the highest ORAC values, and quercetin exhibited the highest PSC values and CAA values.
['Wang|Lifeng|L|', 'Chen|Chao|C|', 'Su|Anxiang|A|', 'Zhang|Yiyi|Y|', 'Yuan|Jian|J|', 'Ju|Xingrong|X|']
D000975:Antioxidants D031742:Coix D005419:Flavonoids D056945:Hep G2 Cells D006801:Humans D010636:Phenols D010936:Plant Extracts D012639:Seeds D053719:Tandem Mass Spectrometry
Phenolic compounds Antioxidant activity Fractionation Defatted adlay seed meal Ferulic acid Rutin Quercetin