Apolipoprotein E4 causes early olfactory network abnormalities and short-term olfactory memory impairments.
While apolipoprotein (Apo) E4 is linked to increased incidence of Alzheimer's disease (AD), there is growing evidence that it plays a role in functional brain irregularities that are independent of AD pathology. However, ApoE4-driven functional differences within olfactory processing regions have yet to be examined. Utilizing knock-in mice humanized to ApoE4 versus the more common ApoE3, we examined a simple olfactory perceptual memory that relies on the transfer of information from the olfactory bulb (OB) to the piriform cortex (PCX), the primary cortical region involved in higher order olfaction. In addition, we have recorded in vivo resting and odor-evoked local field potentials (LPF) from both brain regions and measured corresponding odor response magnitudes in anesthetized young (6-month-old) and middle-aged (12-month-old) ApoE mice. Young ApoE4 compared to ApoE3 mice exhibited a behavioral olfactory deficit coinciding with hyperactive odor-evoked response magnitudes within the OB that were not observed in older ApoE4 mice. Meanwhile, middle-aged ApoE4 compared to ApoE3 mice exhibited heightened response magnitudes in the PCX without a corresponding olfactory deficit, suggesting a shift with aging in ApoE4-driven effects from OB to PCX. Interestingly, the increased ApoE4-specific response in the PCX at middle-age was primarily due to a dampening of baseline spontaneous activity rather than an increase in evoked response power. Our findings indicate that early ApoE4-driven olfactory memory impairments and OB network abnormalities may be a precursor to later network dysfunction in the PCX, a region that not only is targeted early in AD, but may be selectively vulnerable to ApoE4 genotype.
['Peng|Katherine Y|KY|', 'Mathews|Paul M|PM|', 'Levy|Efrat|E|', 'Wilson|Donald A|DA|']
D000375:Aging D000818:Animals D053318:Apolipoprotein E3 D053327:Apolipoprotein E4 D001611:Beta Rhythm D005260:Female D055879:Gene Knock-In Techniques D006801:Humans D008297:Male D008569:Memory Disorders D008570:Memory, Short-Term D008810:Mice, Inbred C57BL D008822:Mice, Transgenic D009043:Motor Activity D009830:Olfactory Bulb D009833:Olfactory Pathways D055696:Olfactory Perception D066195:Piriform Cortex
olfactory bulb piriform cortex olfaction local field potential apolipoprotein E memory deficits