The effect of pH adjusted electrolytes on capillary isoelectric focusing assessed by high-resolution dynamic computer simulation.
The effect of the composition of electrolytes on capillary IEF is assessed for systems with carrier ampholytes covering 2 pH units and with catholytes of decreased pH, anolytes of increased pH and both electrode solutions with adjusted pH values. For electrolytes composed of formic acid as anolyte and ammonium hydroxide as catholyte, simulation is demonstrated to provide the expected IEF system in which analytes with pI values within the formed pH gradient are focused and become immobile. Addition of formic acid to the catholyte results in the formation of an isotachophoretic zone structure that migrates towards the cathode. With ammonium hydroxide added to the anolyte migration occurs towards the anode. In the two cases, all carrier components and amphoteric analytes migrate isotachophoretically as cations or anions, respectively. The data reveal that mM amounts of a counter ion are sufficient to convert an IEF pattern into an ITP system. With increased amounts of the added counter ion, the overall length of the migrating zone structure shrinks, the range of the pH gradient changes, and the migration rate increases. The studied examples indicate that systems of this type reported in the literature should be classified as ITP and not IEF. When both electrolytes are titrated, a non-uniform background electrolyte composed of formic acid and ammonium hydroxide is established in which analytes migrate according to local pH and conductivity without forming IEF or ITP zone structures. Simulation data are in qualitative agreement with previously published experimental data. This article is protected by copyright. All rights reserved.
['Takácsi-Nagy|Anna|A|', 'Kilár|Ferenc|F|https://orcid.org/0000-0001-5737-5514', 'Thormann|Wolfgang|W|https://orcid.org/0000-0002-9762-1609']
Electrolytes Isoelectric focusing Simulation Carrier ampholytes Isotachophoresis