A novel approach for antibody nanocarriers development through hydrophobic ion-pairing complexation.
IgG-Fab fragment, a model antibody protein was hydrophobically modified by a novel approach of ion-pairing complexation. Three different sulphated ion-pairing agents were utilised including sodium dodecyl sulphate, taurocholic acid and dextran sulphate (DS). The formations of hydrophobic ion-pairing (HIP) complexes were dependant on pH and molar ratio of ion-pairing agent to Fab. Aqueous solubilities of HIP complexes were very low compared to Fab alone. In particular, when dextran sulphate was added as ion-pairing agent, formed Fab:DS HIP complexes were least soluble in water. Further, nanoparticles (NPs) loaded with drug and Fab:DS HIP complex were prepared and characterised with respect to encapsulation efficiency and size. We observed significant improvement in encapsulation efficiency for Fab:DS HIP complex-loaded nanoparticles. This study demonstrates a novel approach of formulating antibody-loaded nanoparticles which can also be employed for delivery of large antibodies.
['Patel|Ashaben|A|', 'Gaudana|Ripal|R|', 'Mitra|Ashim K|AK|']
D016264:Dextran Sulfate D004337:Drug Carriers D057927:Hydrophobic and Hydrophilic Interactions D007140:Immunoglobulin Fab Fragments D007074:Immunoglobulin G D049329:Nanostructures D012967:Sodium Dodecyl Sulfate D013656:Taurocholic Acid
hydrophobic ion-pairing complex IgG-Fab fragment protein dextran sulphate nanoparticles Antibody fragment