Effect of fish and krill oil supplementation on glucose tolerance in rabbits with experimentally induced obesity.
PURPOSE
This study was conducted to investigate the effect of fish oil (FO) and krill oil (KO) supplementation on glucose tolerance in obese New Zealand white rabbits.


METHODS
The experiments were carried out with 24 male rabbits randomly divided into four groups: KO-castrated, treated with KO; FO-castrated, treated with FO; C-castrated, non-treated; NC-non-castrated, non-treated. At the end of treatment period (2 months), an intravenous glucose tolerance test (IVGTT) was performed in all rabbits.


RESULTS
Fasting blood glucose concentrations in FO and KO animals were significantly lower than in group C. The blood glucose concentrations in FO- and KO-treated animals returned to initial values after 30 and 60 min of IVGTT, respectively. In liver, carnitine palmitoyltransferase 2 (Cpt2) and 3-hydroxy-3-methyl-glutaryl-CoA synthase 2 (Hmgcs2) genes were significantly increased in FO-fed rabbits compared with the C group. Acetyl-CoA carboxylase alpha (Acaca) expression was significantly reduced in both KO- and FO-fed rabbits. In skeletal muscle, Hmgcs2 and Cd36 were significantly higher in KO-fed rabbits compared with the C group. Acaca expression was significantly lower in KO- and FO-fed rabbits compared with the C group.


CONCLUSION
The present results indicate that FO and KO supplementation decreases fasting blood glucose and improves glucose tolerance in obese New Zealand white rabbits. This could be ascribed to the ameliorated insulin sensitivity and insulin secretion and modified gene expressions of some key enzymes involved in β-oxidation and lipogenesis in liver and skeletal muscle.
['Ivanova|Zhenya|Z|', 'Bjørndal|Bodil|B|', 'Grigorova|Natalia|N|', 'Roussenov|Anton|A|', 'Vachkova|Ekaterina|E|', 'Berge|Kjetil|K|', 'Burri|Lena|L|', 'Berge|Rolf|R|', 'Stanilova|Spaska|S|', 'Milanova|Anelia|A|', 'Penchev|Georgi|G|', 'Vik|Rita|R|', 'Petrov|Vladimir|V|', 'Georgieva|Teodora Mircheva|TM|', 'Bivolraski|Boycho|B|', 'Georgiev|Ivan Penchev|IP|']
D000103:Acetyl-CoA Carboxylase D000818:Animals D001786:Blood Glucose D050260:Carbohydrate Metabolism D002334:Carnitine O-Palmitoyltransferase D019587:Dietary Supplements D033201:Euphausiacea D005395:Fish Oils D005399:Fishes D005786:Gene Expression Regulation D006904:Hydroxymethylglutaryl-CoA Synthase D007328:Insulin D007333:Insulin Resistance D008099:Liver D008297:Male D018482:Muscle, Skeletal D009765:Obesity D011817:Rabbits
Krill oil Glucose tolerance Obesity Fish oil Gene expression Lipogenesis