The contribution of lot-to-lot variation to the measurement uncertainty of an LC-MS-based multi-mycotoxin assay.
Multi-mycotoxin determination by LC-MS is commonly based on external solvent-based or matrix-matched calibration and, if necessary, the correction for the method bias. In everyday practice, the method bias (expressed as apparent recovery RA), which may be caused by losses during the recovery process and/or signal/suppression enhancement, is evaluated by replicate analysis of a single spiked lot of a matrix. However, RA may vary for different lots of the same matrix, i.e., lot-to-lot variation, which can result in a higher relative expanded measurement uncertainty (U r ). We applied a straightforward procedure for the calculation of U r from the within-laboratory reproducibility, which is also called intermediate precision, and the uncertainty of RA (ur,RA). To estimate the contribution of the lot-to-lot variation to U r , the measurement results of one replicate of seven different lots of figs and maize and seven replicates of a single lot of these matrices, respectively, were used to calculate U r . The lot-to-lot variation was contributing to ur,RA and thus to U r for the majority of the 66 evaluated analytes in both figs and maize. The major contributions of the lot-to-lot variation to ur,RA were differences in analyte recovery in figs and relative matrix effects in maize. U r was estimated from long-term participation in proficiency test schemes with 58%. Provided proper validation, a fit-for-purpose U r of 50% was proposed for measurement results obtained by an LC-MS-based multi-mycotoxin assay, independent of the concentration of the analytes.
['Stadler|David|D|', 'Sulyok|Michael|M|', 'Schuhmacher|Rainer|R|', 'Berthiller|Franz|F|', 'Krska|Rudolf|R|']
D002138:Calibration D002853:Chromatography, Liquid D030681:Ficus D009183:Mycotoxins D012015:Reference Standards D015203:Reproducibility of Results D021241:Spectrometry, Mass, Electrospray Ionization D053719:Tandem Mass Spectrometry D035501:Uncertainty D003313:Zea mays
Recovery Relative matrix effect Proficiency test Fit for purpose Matrix mismatch Uncertainty budget