Co-expression of EtMic2 protein and chicken interleukin-18 for DNA vaccine against chicken coccidiosis.
In the present study, a naked EtMIC2 DNA vaccine, a ChIL-18 expression vector and a EtMIC2 and ChIL-18 co-expression DNA vaccine were constructed and their protective efficacies against homologous challenge were compared and evaluated by examining the body weight gain, oocyst shedding, cecal lesion, ACI as well as specific anti-EtMic2 antibody level, the proliferation ability and percentages of CD4+ and CD8+ of splenocytes. The results showed the naked EtMIC2 DNA vaccine could increase the weight gain and decrease the oocyst shedding, but could not alleviate the cecal lesion of immunized chickens compared to unimmunized chickens. Chickens immunized with the co-expression vector pVAX1-MIC2-IL-18 exhibited much improved immune protection against challenge compared to chickens immunized with naked EtMIC2 DNA vaccine, or with naked EtMIC2 DNA vaccine and ChIL-18 expression vector applied separately. These results suggest that the co-expression of ChIL-18 with EtMic2 together could significantly improve the immune protection of the EtMic2 protein.
['Shi|Wenyan|W|', 'Liu|Qing|Q|', 'Zhang|Jie|J|', 'Sun|Jingjing|J|', 'Jiang|Xiyue|X|', 'Geng|Jing|J|', 'Wang|Fangkun|F|', 'Xiao|Yihong|Y|', 'Li|Hongmei|H|', 'Zhao|Xiaomin|X|']
D000818:Animals D015153:Blotting, Western D016516:CD4-CD8 Ratio D002432:Cecum D002645:Chickens D003048:Coccidiosis D017931:DNA Primers D004926:Escherichia coli D020869:Gene Expression Profiling D020382:Interleukin-18 D008856:Microscopy, Fluorescence D033661:Oocysts D010957:Plasmids D011201:Poultry Diseases D016052:Protozoan Vaccines D014611:Vaccination D019444:Vaccines, DNA D015430:Weight Gain
Co-expression EtMIC2 DNA vaccine ChIL-18