miR-214 promotes the proliferation and invasion of osteosarcoma cells through direct suppression of LZTS1.
Previous studies have shown that miR-214 functions either as an oncogene or a tumor suppressor in various human cancer types. The role of this microRNA in osteosarcoma (OS) is presently unclear. Here, we demonstrated that miR-214 is frequently upregulated in OS specimens, compared with noncancerous bone tissues. Bioinformatics analysis further revealed leucine zipper, putative tumor suppressor 1 (LZTS1) as a potential target of miR-214. Expression patterns of miR-214 were inversely correlated with those of LZTS1 mRNA and protein in OS tissues. Data from reporter assays showed that miR-214 directly binds to the 3'-untranslated region (3'-UTR) of LZTS1 mRNA and suppresses expression at both transcriptional and translational levels. In functional assays, miR-214 promoted OS cell proliferation, invasion and tumor growth in nude mice, which could be reversed by overexpression of LZTS1. Taken together, our data provide compelling evidence that miR-214 functions as an onco-miRNA in OS, and its oncogenic effects are mediated chiefly through downregulation of LZTS1.
['Xu|Zhengyu|Z|', 'Wang|Tao|T|']
D020413:3' Untranslated Regions D000818:Animals D001859:Bone Neoplasms D045744:Cell Line, Tumor D049109:Cell Proliferation D004268:DNA-Binding Proteins D015536:Down-Regulation D055785:Gene Knockdown Techniques D064593:Heterografts D006801:Humans D051379:Mice D008807:Mice, Inbred BALB C D008819:Mice, Nude D035683:MicroRNAs D009361:Neoplasm Invasiveness D009857:Oncogenes D012516:Osteosarcoma D012334:RNA, Neoplasm D025521:Tumor Suppressor Proteins D015854:Up-Regulation
miR-214 Osteosarcoma LZTS1