In vitro antioxidant potential and in vivo effects of Schinus terebinthifolia Raddi leaf extract in diabetic rats and determination of chemical composition by HPLC-ESI-MS/MS.
The present study investigated the in vitro and in vivo antioxidant potential and phytochemical composition of Schinus terebinthifolia, which is widely used in folk medicine for various therapeutic purposes. The in vitro analyses indicated that the hydroethanolic extract (HE) had 312.50 ± 0.50 mg GAE/g of total phenols. It also presented anti-DPPH• and anti-ABTS•+ activity, reduced phosphomolybden and metal ions and blocked the bleaching of β-carotene. The HE at concentrations of 3.0 and 2.0 μg/mL had TRAP values of 2.223 ± 0.018 and 1.894 ± 0.026 μM Trolox, respectively. The HE increased the availability of antioxidants in plasma in treated animals in vivo. HPLC-ESI-MS/MS indicated the presence of 11 phenols: cumaric acid, (+)-catechin, myricetin-3-O-glicuronide, kaempferol-3-O-glucoside, myricetin, myricitrin, quercetin, gallic acid, methyl galate, pentagalloyl glucose and ethyl galate. Thus, S. terebinthifolia has potential for the prevention or treatment of diseases that are related to oxidative stress, such as diabetes mellitus.
['Iwanaga|Camila Cristina|CC|', 'Ferreira|Lilian Dos Anjos Oliveira|LDAO|', 'Bernuci|Karine Zanoli|KZ|', 'Fernandez|Carla Maria Mariano|CMM|', 'Lorenzetti|Fabiana Brusco|FB|', 'Sehaber|Camila Caviquioli|CC|', 'Vieira Frez|Flávia Cristina|FC|', 'Bernardes|Sara Santos|SS|', 'Panizzon|Gean Pier|GP|', 'Linde|Giani Andrea|GA|', 'Vieira|Maria do Carmo|MDC|', 'Zanoni|Jacqueline Nelisis|JN|', 'Cortez|Diógenes Aparício Garcia|DAG|']
D027926:Anacardiaceae D000818:Animals D000975:Antioxidants D002851:Chromatography, High Pressure Liquid D003921:Diabetes Mellitus, Experimental D007004:Hypoglycemic Agents D008297:Male D018384:Oxidative Stress D010636:Phenols D010936:Plant Extracts D018515:Plant Leaves D017208:Rats, Wistar D021241:Spectrometry, Mass, Electrospray Ionization D053719:Tandem Mass Spectrometry
oxidative stress Diabetes mellitus medicinal plants phenolic compounds