Acetylation of polysaccharide from Morchella angusticeps peck enhances its immune activation and anti-inflammatory activities in macrophage RAW264.7 cells.
Morchella angusticeps Peck has been recognized as a resource of nutraceuticals and drug discovery. Three acetylated polysaccharides (Ac-PMEP1-3) with appropriate degree of substitution were obtained from Morchella angusticeps Peck, chemically characterized, and cultured with macrophage RAW264.7 cells to evaluate their immune activation and anti-inflammatory activities. Results of ultraviolet-visible spectroscopy and fourier-transform infrared showed these modifications were successful. Compared with the control group, PMEP and Ac-PMEP1-3 enhanced cell proliferation and the production of nitric oxide and tumor necrosis factor-α of RAW264.7 macrophages (cultured without lipopolysaccharide). Compared with PMEP, Ac-PMEP3 enhanced cell viability and NO production by inducing the degradation of cytoplasmic IκBα and nuclear translocation of NF-κB subunit p65 as well as the expression of iNOS and phosphorylated-p38. Moreover, in lipopolysaccharide-stimulated RAW264.7 macrophages, Ac-PMEP3 showed a stronger ability to suppress the overproduction of nitric oxide and tumor necrosis factor-α by down-regulating the level of nuclear NF-κB p65, iNOS, and phosphorylated-p38 and inhibiting the degradation of cytoplasmic IκBα. Therefore, Ac-PMEP enhanced immune activation and anti-inflammatory activities via nuclear factor κB and p38/mitogen-activated protein kinase signaling pathways.
['Yang|Yaxuan|Y|', 'Chen|Jinlong|J|', 'Lei|Lin|L|', 'Li|Fuhua|F|', 'Tang|Yu|Y|', 'Yuan|Ya|Y|', 'Zhang|Yueqiao|Y|', 'Wu|Surui|S|', 'Yin|Ran|R|', 'Ming|Jian|J|']
D000107:Acetylation D000818:Animals D000893:Anti-Inflammatory Agents D001203:Ascomycota D008264:Macrophages D051379:Mice D009569:Nitric Oxide D010587:Phagocytosis D011134:Polysaccharides D000067996:RAW 264.7 Cells D013056:Spectrophotometry, Ultraviolet D017550:Spectroscopy, Fourier Transform Infrared D014409:Tumor Necrosis Factor-alpha
Morchella angusticepes peck Anti-inflammatory RAW264.7 cells Acetylated polysaccharide Nuclear NF-κB Immunomodulatory