Ethanolic extract of Ferula gummosa is cytotoxic against cancer cells by inducing apoptosis and cell cycle arrest.
Ferula gummosa Boiss. has medicinal applications in treating a wide range of diseases including cancer. The objective of this study was to evaluate the antiproliferative activities of the seed and gum extracts of F. gummosa as well as to study the effect of the potent extract on the induction of apoptosis and cell cycle arrest. Our results demonstrated that the ethanolic extract had the lowest IC50 value at 72 h (0.001 ± 1.2 mg/mL) in BHY cells. Moreover, flowcytometry and annexin-V analysis revealed that the ethanolic extract induced apoptosis and cell-cycle arrest in BHY cells at G1/S phase. In addition, colorimetric methods exhibited the highest amount of total phenolics and flavonoids in the aqueous and gum extracts (0.12 ± 0.037, 0.01 ± 2.51 mg/g of dry powder). Generally, the results obtained indicate that F. gummosa ethanol extract may contain effective compounds which can be used as a chemotherapeutic agent.
['Gudarzi|Hoda|H|', 'Salimi|Mona|M|', 'Irian|Saeed|S|', 'Amanzadeh|Amir|A|', 'Mostafapour Kandelous|Hirsa|H|', 'Azadmanesh|Keyhan|K|', 'Salimi|Misha|M|']
D017209:Apoptosis D059447:Cell Cycle Checkpoints D045744:Cell Line, Tumor D005309:Ferula D005419:Flavonoids D006801:Humans D020128:Inhibitory Concentration 50 D010636:Phenols D010936:Plant Extracts
Ferula gummosa cancer apoptosis flowcytometry MTT assay