How to Study the Proteomes and Phosphoproteomes of Anther and Pollen.
Proteomics analysis was a powerful technology for characterizing proteins and protein posttranslational modification (PTMs). Recently, many anther and pollen-related proteomic analyses have been reported, which have expanded our understanding of anther and pollen development and regulation. In this chapter, we describe a detailed, optimized protocol for the separation, digestion, tagging, and subsequent mass spectrometry-based identification and quantification of proteins and phosphoproteins from anther and pollen.
['Zhang|Chi|C|', 'Feng|Xiaobing|X|', 'Hu|Menghui|M|', 'Zhang|Zaibao|Z|']
D002853:Chromatography, Liquid D035264:Flowers D010750:Phosphoproteins D010940:Plant Proteins D011058:Pollen D020543:Proteome D040901:Proteomics D053719:Tandem Mass Spectrometry
Proteome Phosphoproteome Anther Pollen Liquid chromatography–mass spectrometry (LC-MS/MS)