The effects of fatty acid composition on cardiac hypertrophy and function in mouse models of diet-induced obesity.
High-fat diets (HFDs) are used frequently to study the development of cardiac dysfunction in animal models of obesity and diabetes. However, impairment in systolic function, often reported as declining ejection fraction, may not consistently occur in a given time frame which could be contributable to a variety of factors within the experimental design. One major factor may be the amounts of saturated and unsaturated fatty acids (FAs) that are present in the diet. To determine whether the FA content and composition were critical determinants in the development of cardiac dysfunction in response to high-fat feeding, we fed adult, male mice Western diet (45% fat, 60% saturated), Surwit diet (60% fat, 90% saturated), milk-fat-based diet (60% fat, 60% saturated) or high-fat Western diet (HFWD, 60% fat, 32% saturated) for 12 weeks. We report that neither the amount of total fat nor the ratio of saturated to unsaturated FAs in the diets differentially affects body weight and adiposity in mice. In addition, no evidence of systolic dysfunction is present after 12 weeks. Interestingly, the HFWD, with equal parts saturated, monounsaturated and polyunsaturated FAs, induces mild cardiac hypertrophy and diastolic dysfunction after 12 weeks, which coincides with elevated serum levels of arachidonic acid. Our results suggest that the dietary FA content and composition may be a primary determinant of diastolic, but not systolic, dysfunction in animal models of diet-induced obesity.
['Nguyen|Son|S|', 'Shao|Dan|D|', 'Tomasi|Loreta C|LC|', 'Braun|Alyssa|A|', 'de Mattos|Ana Barbosa Marcondes|ABM|', 'Choi|Yong Seon|YS|', 'Villet|Outi|O|', 'Roe|Nathan|N|', 'Halterman|Carliana R|CR|', 'Tian|Rong|R|', 'Kolwicz|Stephen C|SC|']
D050154:Adiposity D000818:Animals D001835:Body Weight D006332:Cardiomegaly D059305:Diet, High-Fat D066273:Diet, Western D004195:Disease Models, Animal D005227:Fatty Acids D008401:Gas Chromatography-Mass Spectrometry D008055:Lipids D008297:Male D008810:Mice, Inbred C57BL D008815:Mice, Inbred Strains D009765:Obesity
Cardiomyopathy Fatty acid profiling Lipid metabolism Lipid overload Mouse strain