Integrative genomic analyses identify WDR12 as a novel oncogene involved in glioblastoma.
Glioblastoma (GBM) is the most malignant primary brain tumor in adults. Due to its invasive nature, it cannot be thoroughly eliminated. WD repeat domain 12 (WDR12) processes the 32S precursor rRNA but cannot affect the synthesis of the 45S/47S primary transcript. In this study, we found that WDR12 is highly expressed in GBM according to the analysis results of mRNA expression by The Cancer Genome Atlas database. The high expression level of WDR12 is dramatically related to shorter overall survival and reduced disease-free survival. Next, we knocked down WDR12 and found that knockdown of WDR12 promoted the apoptosis and inhibited the proliferation by cell biology experiments. Differential expression genes in gene-chip revealed that WDR12 knockdown mainly inhibited cell cycle. Finally, we also found that WDR12 is associated with PLK1 and EZH2 in cell proliferation of GBM. Resumptively, this report showed a possible evidence that WDR12 drove malignant behavior of GBM, whose expression may present a neoteric independent prognostic biomarker in GBM.
['Li|Jun-Liang|JL|', 'Chen|Cheng|C|', 'Chen|Wei|W|', 'Zhao|Ling-Feng|LF|', 'Xu|Xin-Ke|XK|', 'Li|Yang|Y|', 'Yuan|Hong-Yao|HY|', 'Lin|Jin-Rong|JR|', 'Pan|Jun-Ping|JP|', 'Jin|Bi-Lian|BL|', 'Li|Fang-Cheng|FC|0000-0003-0082-0095']
D014408:Biomarkers, Tumor D001932:Brain Neoplasms D018797:Cell Cycle Proteins D045744:Cell Line, Tumor D049109:Cell Proliferation D015972:Gene Expression Regulation, Neoplastic D023281:Genomics D005909:Glioblastoma D006801:Humans D009857:Oncogenes D011379:Prognosis D012333:RNA, Messenger D016601:RNA-Binding Proteins
genomic analyses WDR12 oncogene glioblastoma