Multidrug-resistant Escherichia coli isolated from canine faeces in a public park in Quito, Ecuador.
OBJECTIVES
This study focused on estimating the prevalence of extended-spectrum β-lactamases (ESBLs), plasmid-mediated AmpC β-lactamases, carbapenemases and MCR-1-producing Escherichia coli in canine faeces from a public park in Quito, Ecuador.


METHODS
Phenotypic and genotypic characterisation of E. coli isolated from 50 canine faecal samples recovered from a city park in Quito was performed. In addition, a multiple choice survey was conducted among 50 dog owners.


RESULTS
Of the 50 faecal samples, 20 (40.0%) presented E. coli resistant to ceftriaxone. Moreover, 23 E. coli isolates were recovered for further analysis. All of the isolates showed as multidrug-resistant (MDR) phenotype (resistant to three or more antibiotic families). Resistance to carbapenems, tigecycline and amikacin was not observed. No major clonal relatedness was observed among the resistant isolates. The ESBL genes blaCTX-M-15, blaCTX-M-55 and blaCTX-M-65 were the most common. Two isolates harboured the blaCMY-2 gene and one isolate harboured both mcr-1 and blaCTX-M-65. Statistical analysis showed that older people were more conscious of collecting and disposing of dog faeces than subjects aged <35 years (P < 0.05).


CONCLUSION
The finding of MDR E. coli in dog faeces in a city park in Ecuador illustrates the importance of analysing canine faeces in public settings (e.g. parks, playgrounds) as part of surveillance programmes for MDR E. coli. In addition, this research might be a sentinel sampling method to gain a better understanding of community sources of antibiotic-resistant Enterobacteriaceae at human-animal-environment interfaces.
['Ortega-Paredes|David|D|', 'Haro|Marco|M|', 'Leoro-Garzón|Paula|P|', 'Barba|Pedro|P|', 'Loaiza|Karen|K|', 'Mora|Francisco|F|', 'Fors|Martha|M|', 'Vinueza-Burgos|Christian|C|', 'Fernández-Moreira|Esteban|E|']
D000818:Animals D000900:Anti-Bacterial Agents D004285:Dogs D024901:Drug Resistance, Multiple, Bacterial D004484:Ecuador D004926:Escherichia coli D004927:Escherichia coli Infections D029968:Escherichia coli Proteins D005243:Feces D005838:Genotype D008826:Microbial Sensitivity Tests D000068316:Parks, Recreational D015995:Prevalence D001618:beta-Lactamases
Escherichia coli Canine faeces Public park Ecuador ESBL mcr-1