Curcumin nanoparticles inhibit corneal neovascularization.
UNLABELLED
Corneal neovascularization is a leading cause for compromised vision. Therapeutic prevention of corneal neovascularization is a major clinical challenge, and there is a compelling need to seek effective and safe therapy for this pathology. This study is aimed to evaluate curcumin nanoparticle for prevention of corneal neovascularization. MePEG-PCL nanoparticles were successfully prepared and characterized. The nanoparticle of curcumin has shown increased efficiency in preventing angiogenic sprouting in vitro. Topical delivery of curcumin nanoparticle in the eye showed enhanced retention of curcumin in the cornea, and significant improvement in prevention of corneal neovascularization over free curcumin as graded clinically and by histopathology; suppression in the expression of VEGF, inflammatory cytokines, and MMP was evidenced in the treated cornea. Curcumin inhibited NFκB in LPS-induced corneal cells. Histopathology and scanning electron microscopy showed absence of any adverse change in the corneal structure following application of curcumin nanoparticle. Therefore, we conclude that curcumin nanoparticle can be a potential candidate for prevention of corneal neovascularization.


KEY MESSAGE
• Curcumin nanoparticles show enhanced retention of curcumin in the cornea. • Curcumin NPs suppress the expression of VEGF, inflammatory cytokines, and MMP. • Curcumin NPs prevent corneal neovascularization by suppressing the NFκB pathway. • Curcumin NPs may be a promising candidate for prevention of corneal neovascularization.
['Pradhan|Nirparaj|N|', 'Guha|Rajdeep|R|', 'Chowdhury|Sushovan|S|', 'Nandi|Sudip|S|', 'Konar|Aditya|A|', 'Hazra|Sarbani|S|']
D020533:Angiogenesis Inhibitors D000818:Animals D001013:Aorta, Thoracic D002478:Cells, Cultured D003315:Cornea D060527:Corneal Keratocytes D016510:Corneal Neovascularization D003474:Curcumin D004337:Drug Carriers D005260:Female D020672:I-kappa B Proteins D053583:Interleukin-1beta D008070:Lipopolysaccharides D008297:Male D051379:Mice D000072000:NF-KappaB Inhibitor alpha D016328:NF-kappa B D053758:Nanoparticles D011091:Polyesters D011092:Polyethylene Glycols D017207:Rats, Sprague-Dawley D014409:Tumor Necrosis Factor-alpha D042461:Vascular Endothelial Growth Factor A
Curcumin Neovascularization Cornea