A new method to assay hypoxia-inducible factor-1 based on small molecule binding DNA.
Hypoxia-inducible factor-1 (HIF-1) is among the most important indicators of hypoxia in evaluating severity of many diseases. In this work, a novel method for HIF-1 detection is proposed by using electrochemical techniques based on small molecule binding DNA. In this method, since the designed DNA sequence can specifically bind with either an electroactive small molecule or HIF-1, the signal readout is inversely proportional to HIF-1 concentration, thus a simple and easily-operated method for HIF-1 detection can be developed. With the proposed method, HIF-1 can be determined in a linear range from 5 to 25nM with a detection limit of 2.8nM. Furthermore, the proposed method can be directly used to assay HIF-1 in placenta tissue, and the assay results can reliably reflect the severity of preeclampsia, a very dangerous condition during pregnancy. The proposed method also shows desirable sensitivity, high selectivity and excellent reproducibility, so this method can have potential applications in clinical practice.
['Yang|Nana|N|', 'Li|Hao|H|', 'Yin|Tingting|T|', 'Huang|Yue|Y|', 'Sun|Lizhou|L|', 'Li|Genxi|G|']
D001483:Base Sequence D015374:Biosensing Techniques D004247:DNA D017931:DNA Primers D004566:Electrodes D005260:Female D006046:Gold D006801:Humans D051793:Hypoxia-Inducible Factor 1 D010920:Placenta D011247:Pregnancy
Hypoxia-inducible factor-1 Preeclampsia Biosensor Echinomycin Electroanalysis