Expansion of effector and memory T cells is associated with increased survival in recurrent glioblastomas treated with dendritic cell immunotherapy.
Background
The efficacy of dendritic cell (DC) immunotherapy as a single therapeutic modality for the treatment of glioblastoma (GBM) patients remains limited. In this study, we evaluated in patients with GBM recurrence the immune-mediated effects of DC loaded with autologous tumor lysate combined with temozolomide (TMZ) or tetanus toxoid (TT).


Methods
In the phase I-II clinical study DENDR2, 12 patients were treated with 5 DC vaccinations combined with dose-dense TMZ. Subsequently, in eight patients, here defined as Variant (V)-DENDR2, the vaccine site was preconditioned with TT 24 hours before DC vaccination and TMZ was avoided. As a survival endpoint for these studies, we considered overall survival 9 months (OS9) after second surgery. Patients were analyzed for the generation of effector, memory, and T helper immune response.


Results
Four of 12 DENDR2 patients reached OS9, but all failed to show an immunological response. Five of eight V-DENDR2 patients (62%) reached OS9, and one patient is still alive (OS >30 months). A robust CD8+ T-cell activation and memory T-cell formation were observed in V-DENDR2 OS>9. Only in these patients, the vaccine-specific CD4+ T-cell activation (CD38+/HLA-DR+) was paralleled by an increase in TT-induced CD4+/CD38low/CD127high memory T cells. Only V-DENDR2 patients showed the formation of a nodule at the DC injection site infiltrated by CCL3-expressing CD4+ T cells.


Conclusions
TT preconditioning of the vaccine site and lack of TMZ could contribute to the efficacy of DC immunotherapy by inducing an effector response, memory, and helper T-cell generation.
['Eoli|Marica|M|', 'Corbetta|Cristina|C|', 'Anghileri|Elena|E|', 'Di Ianni|Natalia|N|', 'Milani|Micaela|M|', 'Cuccarini|Valeria|V|', 'Musio|Silvia|S|', 'Paterra|Rosina|R|', 'Frigerio|Simona|S|', 'Nava|Sara|S|', 'Lisini|Daniela|D|', 'Pessina|Sara|S|', 'Maddaloni|Luisa|L|', 'Lombardi|Raffaella|R|', 'Tardini|Maria|M|', 'Ferroli|Paolo|P|', 'DiMeco|Francesco|F|', 'Bruzzone|Maria Grazia|MG|', 'Antozzi|Carlo|C|', 'Pollo|Bianca|B|', 'Finocchiaro|Gaetano|G|', 'Pellegatta|Serena|S|']
glioblastoma dendritic cells immunotherapy tetanus toxoid T-cell memory