An Activatable Afterglow/MRI Bimodal Nanoprobe with Fast Response to H2 S for In Vivo Imaging of Acute Hepatitis.
Accurate detection of hepatic hydrogen sulfide (H2 S) to monitor H2 S-related enzymes' activity is critical for acute hepatitis diagnosis, but remains a challenge due to the dynamic and transient nature of H2 S. Here, we report a H2 S-activatable near-infrared afterglow/MRI bimodal probe F1-GdNP, which shows an "always-on" MRI signal and "off-on" afterglow signal toward H2 S. F1-GdNP shows fast response, high sensitivity and specificity toward H2 S, permitting afterglow imaging of H2 S and evaluation of cystathionine γ-lyase (CSE)'s activity in living mice. We further employ the high spatial-resolution MRI signal of F1-GdNP to track its delivery and accumulation in liver. Importantly, F1-GdNP offers a high signal-to-background ratio (SBR=86.2±12.0) to sensitively report on the increased hepatic H2 S level in the acute hepatitis mice via afterglow imaging, which correlated well with the upregulated CSE activity in the liver, showcasing the good potential of F1-GdNP for monitoring of acute hepatitis process in vivo.
['Zeng|Wenhui|W|', 'Wu|Luyan|L|', 'Ishigaki|Yusuke|Y|', 'Harimoto|Takashi|T|', 'Hu|Yuxuan|Y|', 'Sun|Yidan|Y|', 'Wang|Yuqi|Y|', 'Suzuki|Takanori|T|', 'Chen|Hong-Yuan|HY|', 'Ye|Deju|D|http://orcid.org/0000-0002-9887-0914']
afterglow MRI acute hepatitis hydrogen sulfide cystathionine γ-lyase