Structure comparison and anti-coagulant effects of the glycosaminoglycans from porcine duodenum and jejunum.
To make full use of the porcine sources and develop better choice of novel GAGs as anti-coagulants, two fractions of GAGs from the porcine jejunum (A) and duodenum (C) have been separated & purified. The products were further sulfated to give B and D in order to test the influence of sulfate pattern on the bioactivity. The results showed that the relative molecular weight range of A was 3000-50,000 (Mw, g/mol), whereas C had an average molecular weight of 75,885 (Mw, g/mol). A was identified as a novel heparan sulfate through enzymatic hydrolysis analysis. C was a chondroitin like polysaccharide mainly composed of β-d-GlcA-(1 → 4) and β-d-GalNAc-(1 → 3). A possessed controllable anti-coagulant activity (7 IU/mg) in vitro. The activity of D almost achieved the same magnitude of A. This study demonstrated the anticoagulant potential of the polysaccharides, providing solid foundation for development of anti-coagulants from porcine intestine.
['Yan|Yishu|Y|', 'Ren|Panpan|P|', 'Wu|Qingqing|Q|', 'Liu|Xiaoni|X|', 'Zhang|Zhenqing|Z|', 'Hua|Minyu|M|', 'Xia|Wei|W|', 'Chen|Jinghua|J|']
Anti-coagulant Sulfated Polysaccharide Porcine intestine Anti-thrombotic activity Heparin