In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway.
The sensing apparatus of the Bacillus subtilis chemotaxis pathway involves a complex consisting of chemoreceptors, the CheA histidine kinase, and the CheV and CheW adaptor proteins. Attractants and repellents alter the rate of CheA autophosphorylation, either by directly binding the receptors or by indirectly interacting with them through intermediate binding proteins. We describe an in vitro assay for measuring receptor-kinase activity in B. subtilis. This assay has been used to investigate the mechanism of signal transduction in B. subtilis chemotaxis and the disparate mechanisms employed by this bacterium for sensory adaptation and gradient sensing.
['Walukiewicz|Hanna E|HE|', 'Ordal|George W|GW|', 'Rao|Christopher V|CV|']
D001412:Bacillus subtilis D001426:Bacterial Proteins D001681:Biological Assay D002630:Chemotactic Factors D002633:Chemotaxis D008565:Membrane Proteins D010766:Phosphorylation D015398:Signal Transduction
Bacillus subtilis Chemotaxis Chemoreceptors CheA Kinase assay