Melanin and humic acid-like polymer complex from olive mill waste waters. Part I. Isolation and characterization.
A water soluble humic acid and melanin-like polymer complex (OMWW-ASP) was isolated from olive mill waste waters (OMWW) by ammonium sulfate fractionation to be used as natural additive in food preparations. The dark polymer complex was further characterized by a variety of biochemical, physicochemical and spectroscopic techniques. OMWW-ASP is composed mainly of proteins associated with polyphenols and carbohydrates and the distribution of its relative molecular size was determined between about 5 and 190 kDa. SDS-PAGE shows the presence of a well separated protein band of 21.3 kDa and a low molecular weight peptide. The OMWW-ASP complex exhibits a monotonically increasing UV-Vis absorption spectrum and it contains stable radicals. Antioxidant activity measurements reveal the ability of the OMWW protein fraction to scavenge both the cationic 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(+)) radical, as well as the stable nitroxide free radical 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL).
['Khemakhem|Maissa|M|', 'Papadimitriou|Vassiliki|V|', 'Sotiroudis|Georgios|G|', 'Zoumpoulakis|Panagiotis|P|', 'Arbez-Gindre|Cécile|C|', 'Bouzouita|Nabiha|N|', 'Sotiroudis|Theodore G|TG|']
D000975:Antioxidants D001704:Biopolymers D002412:Cations D006812:Humic Substances D007220:Industrial Waste D008543:Melanins D031658:Olea D010084:Oxidation-Reduction D010940:Plant Proteins D059808:Polyphenols D062065:Waste Water
Melanin Ammonium sulfate fractionation Humic acid size measurements Oil mill waste water Scavenging activity Spectroscopic characterization