Hyperbaric oxygen-induced long non-coding RNA MALAT1 exosomes suppress MicroRNA-92a expression in a rat model of acute myocardial infarction.
Hyperbaric oxygen (HBO) improves angiogenesis. The effect of HBO on metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a pro-angiogenic long non-coding RNA, in cardiac myocyte-derived exosomes and acute myocardial infarction (AMI) is unknown. We aimed to investigate whether MALAT1 is altered in cardiac myocyte-derived exosomes in response to HBO as well as the molecular regulatory mechanisms of MALAT1 in cardiac myocytes treated with HBO. Cardiac myocytes were cultured, and HBO was applied at 2.5 atmosphere absolute in a hyperbaric chamber. Exosomes were extracted from the culture media. A rat model of AMI generated by the ligation of the left anterior descending artery was used. HBO significantly increased MALAT1 expression in cardiac myocytes and HBO-induced MALAT1 and exosomes attenuated miR-92a expression after myocardial infarction. Expression of krüppel-like factor 2 (KLF2) and CD31 was significantly decreased after infarction and HBO-induced exosomes significantly reversed the expression. Silencing of MALAT1 using MALAT1-locked nucleic acid GapmeR significantly attenuated KLF2 and CD31 protein expression after infarction induced by HBO-induced exosomes. HBO-induced exosomes also decreased infarct size significantly. HBO-induced exosomes from cardiac myocytes up-regulate MALAT1 to suppress miR-92a expression and counteract the inhibitory effect of miR-92a on KLF2 and CD31 expression in left ventricular myocardium after myocardial infarction to enhance neovascularization.
['Shyu|Kou-Gi|KG|0000-0002-5710-8640', 'Wang|Bao-Wei|BW|', 'Fang|Wei-Jen|WJ|', 'Pan|Chun-Ming|CM|', 'Lin|Chiu-Mei|CM|']
D000818:Animals D004195:Disease Models, Animal D004452:Echocardiography D055354:Exosomes D020869:Gene Expression Profiling D006439:Hemodynamics D006931:Hyperbaric Oxygenation D000860:Hypoxia D051741:Kruppel-Like Transcription Factors D008297:Male D035683:MicroRNAs D009203:Myocardial Infarction D009206:Myocardium D032383:Myocytes, Cardiac D009389:Neovascularization, Pathologic D019408:Platelet Endothelial Cell Adhesion Molecule-1 D062085:RNA, Long Noncoding D051381:Rats D017208:Rats, Wistar
MALAT1 exosome myocardial infarction hyperbaric oxygen