Pharmacokinetics and Tissue Distribution of Folate-Decorated Human Serum Albumin Loaded With Nano-Hydroxycamptothecin for Tumor Targeting.
The goal of this work is to develop the method of preparing folate (FA)-decorated human serum albumin (HSA) loaded with nano-hydroxycamptothecin (nHCPT) nanoparticles (NPs) (FA-HSA-nHCPT-NPs) and to explore its antitumor activity in vivo and in vitro. FA-HSA-nHCPT-NPs were obtained by preparing nHCPT by a high-pressure homogenization technique followed with an anti-solvent method. The drug-loading efficiency of the FA-HSA-nHCPT-NPs was 7.8%. We adopted the human breast cancer cells (FA receptor-expressing MCF-7 cells) and BALB/c mice inoculated with human MCF-7 cells to determine the antitumor activity of FA-HSA-nHCPT-NPs in vitro and in vivo, respectively. The antitumor activity of FA-HSA-nHCPT-NPs was stronger than that of the raw HCPT in both conditions. Tissue distribution analysis showed that the FA-HSA-nHCPT-NPs carried more HCPT to tumors than the raw HCPT. The tumor inhibitory rate of FA-HSA-nHCPT-NPs was much higher compared with the raw HCPT. Th7us, the FA-HSA-nHCPT-NPs could serve as a viable delivery system with an obvious target effect on tumor.
['Wang|Wenchao|W|', 'Liang|Hui|H|', 'Sun|Baihe|B|', 'Xu|Jialin|J|', 'Zeng|Zhen|Z|', 'Zhao|Xiaojun|X|', 'Li|Qingyong|Q|']
D000818:Animals D001943:Breast Neoplasms D002166:Camptothecin D004305:Dose-Response Relationship, Drug D004337:Drug Carriers D016503:Drug Delivery Systems D005260:Female D005492:Folic Acid D006801:Humans D061986:MCF-7 Cells D051379:Mice D008807:Mice, Inbred BALB C D008819:Mice, Nude D053758:Nanoparticles D000075462:Serum Albumin, Human D014018:Tissue Distribution D023041:Xenograft Model Antitumor Assays
tissue distribution folate-decorated human serum albumin hydroxycamptothecin targeted delivery system