Nonexpanded Adipose Stromal Vascular Fraction Local Therapy on Peripheral Nerve Regeneration Using Allografts.
AIM OF THE STUDY
Adipose tissue possesses a population of multi-potent stem cells which can be differentiated to a Schwann cell phenotype and may be of benefit for treatment of peripheral nerve injuries. Effects of local therapy of nonexpanded adipose stromal vascular fraction (SVF) on peripheral nerve regeneration was studied using allografts in a rat sciatic nerve model.


MATERIALS AND METHODS
Thirty male white Wistar rats were divided into three experimental groups (n = 10), randomly: Sham-operated group (SHAM), allograft group (ALLO), SVF-treated group (ALLO/SVF). In SHAM group left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In the ALLO group the left sciatic nerve was exposed through a gluteal muscle incision and transected proximal to the tibio-peroneal bifurcation where a 10 mm segment was excised. The same procedure was performed in the ALLO/SVF group. The harvested nerves of the rats of ALLO group were served as allograft for ALLO/SVF group and vice versa. The SHAM and ALLO groups received 100 μL phosphate buffered saline and the ALLO/SVF group received 100 μL SVF (2.25 ± 0.45 × 10(7) cells) locally where the grafting was performed.


RESULTS
Behavioral, functional, biomechanical, and gastrocnemius muscle mass showed earlier regeneration of axons in ALLO/SVF than in ALLO group (p < .05). Histomorphometic and immunohistochemical studies also showed earlier regeneration of axons in ALLO/SVF than in ALLO group (p < .05).


CONCLUSIONS
Administration of nonexpanded SVF could accelerate functional recovery after nerve allografting in sciatic nerve. It may have clinical implications for the surgical management of patients after nerve transection.
['Mohammadi|Rahim|R|', 'Mehrtash|Moein|M|', 'Mehrtash|Moeid|M|', 'Sajjadi|Seyedeh-Sepideh|SS|']
D000273:Adipose Tissue D064591:Allografts D000818:Animals D002454:Cell Differentiation D004195:Disease Models, Animal D006801:Humans D008297:Male D039902:Multipotent Stem Cells D018482:Muscle, Skeletal D009416:Nerve Regeneration D059348:Peripheral Nerve Injuries D011897:Random Allocation D051381:Rats D017208:Rats, Wistar D020127:Recovery of Function D012583:Schwann Cells D012584:Sciatic Nerve D017154:Stromal Cells D014184:Transplantation, Homologous
local allograft SVF rat sciatic Peripheral nerve repair