Fetal and neonatal genistein exposure aggravates to interfere with ovarian follicle development of obese female mice induced by high-fat diet.
With epidemic of obesity, it affects aspects of female reproduction. Genistein could ameliorate obesity in people and animals, but might exert adverse effects on the female reproductive system. To evaluate the effects of fetal and neonatal genistein exposure on the ovarian health of F1 obese female mice with obesity induced by high-fat diet after weaning, we simulated a diet-induced obesity model to observe and determine biological effects of genistein exposure on the ovarian follicle of overfed female mice. Results showed that F1 female mice with obesity induced by high-fat diet significantly prolonged the estrus cycle, disrupted sex hormonal balance and ovarian follicle development after they were exposed to 25 mg/kg b.w./day of genistein during the fetal and neonatal stages. Genistein significantly up-regulated the ovarian mRNA expression of estrogen receptor beta in F1 obese female mice, and high-fat diet influenced the ovarian mRNA expression of estrogen receptor alpha, luteinizing hormone receptor and follicle-stimulating hormone receptor. Hence, genistein exposure from the fetal stage might increase the risk of reproductive diseases in obese females in later life. Thus, the long-term risks of genistein to obese females should be thoroughly assessed.
['Liu|Xin|X|', 'Li|Fenfen|F|', 'Xie|Jianhua|J|', 'Huang|Danfei|D|', 'Xie|Mingyong|M|']
D000818:Animals D000831:Animals, Newborn D059305:Diet, High-Fat D004958:Estradiol D047629:Estrogen Receptor beta D030762:Estrous Cycle D005260:Female D005333:Fetus D005640:Follicle Stimulating Hormone D015870:Gene Expression D019833:Genistein D007986:Luteinizing Hormone D008813:Mice, Inbred ICR D009765:Obesity D006080:Ovarian Follicle D011247:Pregnancy D012333:RNA, Messenger
Genistein Ovarian follicle Obesity Fetal and neonatal exposure Reproductive development