Islet-like clusters derived from skeletal muscle-derived stem/progenitor cells for autologous transplantation to control type 1 diabetes in mice.
A population of muscle-derived stem/progenitor cells (MDSPCs) contained in skeletal muscle is responsible for muscle regeneration. MDSPCs from mouse muscle have been shown to be capable of differentiating into pancreatic islet-like cells. However, the potency of MDSPCs to differentiate into functional islet-like cluster remains to be confirmed. The therapeutic potential of autologous MDSPCs transplantation on type 1 diabetes still remains unclear. Here, we investigated a four-stage method to induce the differentiation of MDSPCs into insulin-producing clusters in vitro, and tested the autologous transplantation to control type 1 diabetes in mice. MDSPCs isolated from the skeletal muscles of mice possessed the ability to form islet-like clusters through several stages of differentiation. The expressions of pancreatic progenitor-related genes, insulin, and islet-related genes were significantly upregulated in islet-like clusters determined by the quantitative reverse transcription polymerase chain reaction. The autologous islet-like clusters transplantation effectively improved hyperglycaemia and glucose intolerance and increased the survival rate in streptozotocin-induced diabetic mice without the use of immunosuppressants. Taken together, these results provide evidence that MDSPCs from murine muscle tissues are capable of differentiating into insulin-producing clusters, which possess insulin-producing ability in vitro and in vivo, and have the potential for autologous transplantation to control type 1 diabetes.
['Lan|Kuo-Cheng|KC|', 'Wang|Ching-Chia|CC|', 'Yen|Yuan-Peng|YP|', 'Yang|Rong-Sen|RS|', 'Liu|Shing-Hwa|SH|', 'Chan|Ding-Cheng|DC|']
D000818:Animals D064592:Autografts D002454:Cell Differentiation D003921:Diabetes Mellitus, Experimental D003922:Diabetes Mellitus, Type 1 D007515:Islets of Langerhans D016381:Islets of Langerhans Transplantation D008297:Male D051379:Mice D008813:Mice, Inbred ICR D032448:Myoblasts, Skeletal
islet-like clusters autologous transplantation differentiation Diabetes mellitus muscle-derived stem cells