Robust Induced Presynapse on Artificial Substrates as a Neural Interfacing Method.
Over the recent years, the development of neural interface systems has stuck to using electrical cues to stimulate neurons and read out neural signals, although neurons relay signals via chemical release and recognition at synapses. In addition, conventional neural interfaces are vulnerable to cell migration and glial encapsulation due to the absence of connection anchoring the neuron into the device unlike synapses, which are firmly sustained by protein bonding. To close this discrepancy, we conducted an intensive investigation into the induced synapse interface by employing engineered synaptic proteins from a neural interface perspective. The strong potential of induced synaptic differentiation as an emerging neural interfacing technique is demonstrated by exploring its structural features, chemical release kinetics, robustness, and scalability to the brain tissue. We show that the exocytosis kinetics of induced synapses is similar to that of endogenous synapses. Moreover, induced synapses show remarkable stability, despite cell migration and growth. The synapse-inducing technique has broad applications to cultured hippocampal and cortex tissues and suggests a promising method to integrate neural circuits with digital elements.
['Jeon|Joohee|J|', 'Oh|Min-Ah|MA|', 'Cho|Wonkyung|W|', 'Yoon|Sun-Heui|SH|', 'Kim|Ji Yong|JY|', 'Chung|Taek Dong|TD|http://orcid.org/0000-0003-1092-8550']
D000818:Animals D015816:Cell Adhesion Molecules, Neuronal D002478:Cells, Cultured D002540:Cerebral Cortex D004622:Embryo, Mammalian D005089:Exocytosis D057809:HEK293 Cells D006624:Hippocampus D006801:Humans D055767:Immobilized Proteins D007700:Kinetics D008855:Microscopy, Electron, Scanning D051381:Rats D017207:Rats, Sprague-Dawley D011994:Recombinant Proteins D013379:Substrate Specificity D013569:Synapses D059008:Time-Lapse Imaging
neural interface induced synapse organotypically cultured brain slice primary cultured neuron protein engineering surface modification synaptic activity