A polymorphism of the TIM-1 IgV domain: implications for the susceptibility to filovirus infection.
Filoviruses, including Ebola and Marburg viruses, cause severe hemorrhagic fever in humans and nonhuman primates with mortality rates of up to 90%. Human T-cell immunoglobulin and mucin domain 1 (TIM-1) is one of the host proteins that have been shown to promote filovirus entry into cells. In this study, we cloned TIM-1 genes from three different African green monkey kidney cell lines (Vero E6, COS-1, and BSC-1) and found that TIM-1 of Vero E6 had a 23-amino acid deletion and 6 amino acid substitutions compared with those of COS-1 and BSC-1. Interestingly, Vero E6 TIM-1 had a greater ability to promote the infectivity of vesicular stomatitis viruses pseudotyped with filovirus glycoproteins than COS-1-derived TIM-1. We further found that the increased ability of Vero E6 TIM-1 to promote virus infectivity was most likely due to a single amino acid difference between these TIM-1s. These results suggest that a polymorphism of the TIM-1 molecules is one of the factors that influence cell susceptibility to filovirus infection, providing a new insight into the molecular basis for the filovirus host range.
['Kuroda|Makoto|M|', 'Fujikura|Daisuke|D|', 'Noyori|Osamu|O|', 'Kajihara|Masahiro|M|', 'Maruyama|Junki|J|', 'Miyamoto|Hiroko|H|', 'Yoshida|Reiko|R|', 'Takada|Ayato|A|']
D000595:Amino Acid Sequence D000818:Animals D019556:COS Cells D002522:Chlorocebus aethiops D003001:Cloning, Molecular D016563:Filoviridae D018702:Filoviridae Infections D005434:Flow Cytometry D020022:Genetic Predisposition to Disease D049452:Green Fluorescent Proteins D057809:HEK293 Cells D006801:Humans D007668:Kidney D008562:Membrane Glycoproteins D008969:Molecular Sequence Data D016296:Mutagenesis D011110:Polymorphism, Genetic D017434:Protein Structure, Tertiary D011991:Receptors, Virus D017386:Sequence Homology, Amino Acid D014709:Vero Cells
Polymorphism TIM-1 Susceptibility Filovirus Entry Receptor