Tetherin antagonism by V proteins is a common trait among the genus Rubulavirus.
Tetherin (BST-2/CD317/HM1.24) is an anti-viral factor that restricts the budding of several enveloped viruses. Most of these viruses have evolved to encode tetherin antagonists. Our previous study demonstrated that the growth of human parainfluenza virus type 2 (hPIV-2), a member of the genus Rubulavirus in the family Paramyxoviridae, was inhibited by tetherin, and its V protein decreases the amount of cell surface tetherin by the interaction. In the present study, we investigated whether tetherin inhibits the growth of other rubulaviruses including PIV-5, mumps virus (MuV), simian virus 41, and hPIV-4, and whether their V proteins act as tetherin antagonists. Plaque assay demonstrated that the growth of PIV-5 and MuV was inhibited by tetherin. Flow cytometry and immunoblot analyses revealed that the infection of PIV-5 and MuV caused reduction of cell surface tetherin without affecting total amount of tetherin. Immunoprecipitation analysis showed that all V proteins of rubulaviruses tested bound to tetherin. These results suggest that tetherin antagonism by V proteins is common among the genus Rubulavirus.
['Ohta|Keisuke|K|', 'Matsumoto|Yusuke|Y|', 'Ito|Morihiro|M|', 'Nishio|Machiko|M|http://orcid.org/0000-0002-5311-5798']
D000818:Animals D015703:Antigens, CD D019556:COS Cells D002522:Chlorocebus aethiops D005434:Flow Cytometry D058851:GPI-Linked Proteins D054884:Host-Pathogen Interactions D006801:Humans D057131:Immune Evasion D015151:Immunoblotting D047468:Immunoprecipitation D050505:Mutant Proteins D011485:Protein Binding D019213:Rubulavirus D014709:Vero Cells D010948:Viral Plaque Assay D014764:Viral Proteins
Tetherin V protein Rubulavirus Paramyxovirus