Hierarchical ZIF-8 toward Immobilizing Burkholderia cepacia Lipase for Application in Biodiesel Preparation.
A hierarchical mesoporous zeolitic imidazolate framework (ZIF-8) was processed based on cetyltrimethylammonium bromide (CTAB) as a morphological regulating agent and amino acid (l-histidine) as assisting template agent. Burkholderia cepacia lipase (BCL) was successfully immobilized by ZIF-8 as the carrier via an adsorption method (BCL-ZIF-8). The immobilized lipase (BCL) showed utmost activity recovery up to 1279%, a 12-fold boost in its free counterpart. BCL-ZIF-8 was used as a biocatalyst in the transesterification reaction for the production of biodiesel with 93.4% yield. There was no significant lowering of conversion yield relative to original activity for BCL-ZIF-8 when continuously reused for eight cycles. This work provides a new outlook for biotechnological importance by immobilizing lipase on the hybrid catalyst (ZIF-8) and opens the door for its uses in the industrial field.
['Adnan|Miaad|M|0000-0002-9119-9419', 'Li|Kai|K|', 'Wang|Jianhua|J|', 'Xu|Li|L|', 'Yan|Yunjun|Y|']
D056804:Biofuels D016956:Burkholderia cepacia D002384:Catalysis D000077286:Cetrimonium D002593:Cetrimonium Compounds D004800:Enzymes, Immobilized D004951:Esterification D006639:Histidine D008049:Lipase D017641:Zeolites
hierarchical ZIF-8 Burkholderia cepacia lipase (BCL) adsorption method biodiesel production hexahedral ZIF-8 surfactant