Profiling neuron-autonomous lncRNA changes upon ischemia/reperfusion injury.
Extensive changes of neuronal transcriptome occur post ischemic stroke and during the following reperfusion. Although numerous studies focused on transcriptome changes of mRNAs associated with ischemic stroke, little is known about whether and how long non-coding RNAs (lncRNAs), which play critical roles in cellular homeostasis, are involved in this process. In this study, we performed high throughput screening to analyze expression changes of lncRNAs in primarily cultured hippocampal neurons under an oxygen-glucose deprivation/reperfusion (OGD/R) condition at 0 h, 6 h, 12 h, and 18 h, respectively. Knock down of one validated lncRNAs (Tnxa-ps1) promoted neuronal survival by inhibiting apoptosis. Coding non-coding co-expression network analysis revealed that the expression of Tnxa-ps1 was highly correlated with changes of a particular group of genes, many of which are associated with neural protection. Finally, we showed that down-regulation of Tnxa-ps1 reversed the expression changes of four mRNAs post OGD/R, revealing a regulatory effect between Tnxa-ps1 and selected genes. Together, our data revealed possible participation of lncRNAs in the pathophysiology of OGD/R and thereby provided new insights into the studies of potential therapeutic targets for ischemic stroke.
['Li|Haiying|H|', 'Wu|Youjia|Y|', 'Suo|Guihai|G|', 'Shen|Feifei|F|', 'Zhen|Yuqin|Y|', 'Chen|Xia|X|', 'Lv|Haitao|H|']
D000818:Animals D017209:Apoptosis D002470:Cell Survival D002478:Cells, Cultured D020869:Gene Expression Profiling D055785:Gene Knockdown Techniques D006624:Hippocampus D009474:Neurons D062085:RNA, Long Noncoding D012333:RNA, Messenger D034741:RNA, Small Interfering D051381:Rats D015427:Reperfusion Injury D019063:Tenascin
lncRNA Ischemia/reperfusion Stroke Oxygen-glucose deprivation/reperfusion Tnxa-ps1 Neuronal survival