Adipose tissue complement factor B promotes adipocyte maturation.
OBJECTIVES
It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism.


METHODS
We investigated the expression level of complement system-related genes in adipocytes. To understand the role of CfB in adipocyte, we performed Cfb overexpression in 3T3-L1 preadipocytes and generated adipocyte-specific Cfb transgenic mice.


RESULTS
Cfb expression was markedly enhanced in 3T3-L1 adipocytes co-cultured with macrophages following endotoxin stimulation. In Cfb-overexpressing cells, the expression of adipocyte differentiation/maturation-related genes encoding peroxisome proliferator-activated receptor γ (Pparγ), adipocyte Protein 2 and perilipin was significantly enhanced. Cfb transgenic mice showed a marked increase in the expression of genes encoding Pparγ, perilipin, sterol regulatory element-binding protein 1 c, and Cd36 in the subcutaneous adipose tissue.


CONCLUSIONS
CfB plays a crucial role in late-phase of adipocyte differentiation and subsequent lipid droplet formation.
['Matsunaga|Hiroaki|H|', 'Iwashita|Misaki|M|', 'Shinjo|Takanori|T|', 'Yamashita|Akiko|A|', 'Tsuruta|Mitsudai|M|', 'Nagasaka|Shoichiro|S|', 'Taniguchi|Ataru|A|', 'Fukushima|Mitsuo|M|', 'Watanabe|Naoya|N|', 'Nishimura|Fusanori|F|']
D041721:3T3-L1 Cells D017667:Adipocytes D050156:Adipogenesis D000273:Adipose Tissue D000818:Animals D002454:Cell Differentiation D049109:Cell Proliferation D002478:Cells, Cultured D011415:Complement Factor B D007113:Immunity, Innate D066292:Lipid Droplets D008297:Male D051379:Mice D008822:Mice, Transgenic
Complement factor B Adipocyte maturation Obesity