Novel electrochemical immune sensor based on Hep-PGA-PPy nanoparticles for detection of α-Fetoprotein in whole blood.
A simple and accurate immune sensor for quantitative detection of α-Fetoprotein (AFP) was developed based on the immobilization of antigen on the surface of Hep-PGA-PPy nanoparticles modified glassy carbon electrodes (GCE). The obtained Hep-PGA-PPy nanoparticles were characterized by fourier transform infrared (FT-IR) spectra and transmission electron microscopy (TEM). And the blood compatibility of Hep-PGA-PPy nanoparticles was investigated by in vitro coagulation tests, hemolysis assay and whole blood adhesion tests. Combining the conductive property of polypyrrole (PPy) and the biocompatibility of heparin (Hep), the Hep-PGA-PPy nanoparticles could improve not only the anti-biofouling effect the electrode, but also improved the electrochemical properties of the immune sensor. Under optimal conditions, the proposed immune sensor could detect AFP in a linear range from 0.1 to 100 ng mL-1 with a detection limit of 0.099 ng mL-1 at the signal-to-noise ratio of 3, and it also possessed good reproducibility and storage stability. Furthermore, the detection of AFP in five human blood samples also showed satisfactory accuracy with low relative errors. Thus, the developed immune sensor which showed acceptable reproducibility, selectivity, stability and accuracy could be potentially used for the detection of whole blood samples directly.
['Xu|Tingting|T|', 'Chi|Bo|B|', 'Gao|Jian|J|', 'Chu|Meilin|M|', 'Fan|Wenlu|W|', 'Yi|Meihui|M|', 'Xu|Hong|H|', 'Mao|Chun|C|']
D015374:Biosensing Techniques D055664:Electrochemical Techniques D004566:Electrodes D006493:Heparin D006801:Humans D057230:Limit of Detection D046529:Microscopy, Electron, Transmission D053758:Nanoparticles D011099:Polyglutamic Acid D011108:Polymers D011758:Pyrroles D015203:Reproducibility of Results D017550:Spectroscopy, Fourier Transform Infrared D000509:alpha-Fetoproteins
Electrochemical immune sensor Hep-PGA-PPy nanoparticles α-Fetoprotein Anti-biofouling Label-free