Characterization of a degradable polar hydrophobic ionic polyurethane with circulating angiogenic cells in vitro.
This study investigated the interaction of human circulating angiogenic cells (CACs) with a degradable polar hydrophobic ionic polyurethane (D-PHI) which has been previously shown to exhibit anti-inflammatory character and favorable interactions with human endothelial cells (ECs). Given the implication of the CACs in microvessel development it was of intrinsic interest to expand our knowledge of D-PHI biocompatibility with this relevant primary cell involved in angiogenesis. The findings will be compared to a well-established benchmark substrate for CACs, fibronectin-coated tissue culture polystyrene (TCPS). Immunoblotting analysis showed that CACs were a heterogeneous population of cells composed mostly of monocytic cells expressing the CD14 marker. Assessment of the cytokine release profile, using ELISA, showed that D-PHI supported a higher concentration of interleukin-10 (IL-10) when compared to the concentration of tumor necrosis factor alpha, which is indicative of an anti-inflammatory phenotype, and was different from the response with TCPS. It was found that the CACs were attached to D-PHI and remained viable and functional (nitric oxide production) during the seven days of culture. However, there did not appear to be any significant proliferation on D-PHI, contrary to the CAC growth on fibronectin-coated TCPS. It was concluded that D-PHI displayed some of the qualities suitable to enable the retention of CACs onto this substrate, as well as maintaining an anti-inflammatory phenotype, characteristics which have been reported to be important for angiogenesis in vivo.
['Mathieu|Eva|E|', 'Battiston|Kyle G|KG|', 'McBane|Joanne E|JE|', 'Davidson|Lien|L|', 'Suuronen|Erik J|EJ|', 'Santerre|J Paul|JP|', 'Labow|Rosalind S|RS|']
D001672:Biocompatible Materials D002448:Cell Adhesion D002470:Cell Survival D016207:Cytokines D005353:Fibronectins D006801:Humans D057927:Hydrophobic and Hydrophilic Interactions D066298:In Vitro Techniques D016753:Interleukin-10 D018950:Lipopolysaccharide Receptors D008855:Microscopy, Electron, Scanning D009000:Monocytes D018919:Neovascularization, Physiologic D009569:Nitric Oxide D019408:Platelet Endothelial Cell Adhesion Molecule-1 D011140:Polyurethanes D014409:Tumor Necrosis Factor-alpha
degradable polyurethane circulating angiogenic cells monocytes vascular graft