NKILA inhibits NF-κB signaling and suppresses tumor metastasis.
The long non-coding RNA (lncRNA) NKILA (nuclear transcription factor NF-κB interacting lncRNA) functions as a suppressor in human breast cancer and tongue cancer. However, the clinical significance and biological roles of NKILA in esophageal squamous cell carcinoma (ESCC) remain unknown. In this study, we showed that NKILA was downregulated in ESCC tissues and cancer cells compared with their normal counterparts. Low NKILA expression correlated with large tumor size and advanced TNM stage, and predicted poor overall and disease-free survival of ESCC patients. Further loss- and gain-of-function assays indicated that NKILA inhibited proliferation and migration of ESCC cells in vitro, suppressed tumor growth and lung metastasis in vivo. Mechanistically, NKILA could inhibit phosphorylation of IκBα, suppress p65 nuclear translocation and downregulate the expression of NF-κB target genes in ESCC cells. These results suggest NKILA could suppress malignant development of ESCC via abrogation of the NF-κB signaling and may potentially serve as a prognostic marker for ESCC.
['Ke|Shun|S|', 'Li|Rui-Chao|RC|', 'Meng|Fan-Kai|FK|', 'Fang|Ming-Hao|MH|']
D000368:Aged D000818:Animals D014408:Biomarkers, Tumor D045744:Cell Line, Tumor D049109:Cell Proliferation D015536:Down-Regulation D004938:Esophageal Neoplasms D000077277:Esophageal Squamous Cell Carcinoma D005260:Female D015870:Gene Expression D020868:Gene Silencing D006801:Humans D008297:Male D051379:Mice D008807:Mice, Inbred BALB C D008875:Middle Aged D009361:Neoplasm Invasiveness D009362:Neoplasm Metastasis D016016:Proportional Hazards Models D062085:RNA, Long Noncoding D060888:Real-Time Polymerase Chain Reaction D025521:Tumor Suppressor Proteins
NKILA NF-κB metastasis long non-coding RNA esophageal squamous cell carcinoma