Anti-Inflammatory Properties of Bellevalia saviczii Root Extract and Its Isolated Homoisoflavonoid (Dracol) Are Mediated by Modification on Calcium Signaling.
Bellevalia saviczii is a medicinal plant used as anti-rheumatic and anti-inflammatory herbal remedy in Iraqi-Kurdistan. The aim of this study was to evaluate the anti-inflammatory activity of its extract and the isolated homoisoflavonoid (Dracol) by studying the Ca2+-dependent NF-kB pathway. Nuclear translocation of p65 NF-kB subunit, as parameter of NF-kB activation, was visualized in human leukemic monocytes by immunofluorescence and Western blot analyses, after cell treatment with B. saviczii root extract or Dracol followed by Lipopolysaccharide stimulation. In parallel, Ca2+ signals responsible for NF-kB activation and levels of inflammatory cytokines were investigated. LPS-induced p65 translocation was evident in monocytes and both treatments, in particular that with Dracol, were able to counteract this activation. Intracellular Ca2+ oscillations were halted and the cytokine release reduced. These results confirm the traditional anti-inflammatory efficacy of B. saviczii and identify one of the molecules in the extract which appears to be responsible of this action.
['Savio|Monica|M|', 'Ibrahim|Mohammed Farhad|MF|', 'Scarlata|Chiara|C|', 'Orgiu|Matteo|M|', 'Accardo|Giuseppe|G|', 'Sardar|Abdullah Shakur|AS|', 'Moccia|Francesco|F|0000-0003-0010-0098', 'Stivala|Lucia Anna|LA|', 'Brusotti|Gloria|G|0000-0001-7786-3769']
D000893:Anti-Inflammatory Agents D000070381:Asparagales D020013:Calcium Signaling D002470:Cell Survival D005591:Chemical Fractionation D016207:Cytokines D006801:Humans D007529:Isoflavones D008070:Lipopolysaccharides D008262:Macrophage Activation D008264:Macrophages D010936:Plant Extracts D018517:Plant Roots D010946:Plants, Medicinal
Bellevalia saviczii NF-kB Ca2+ signaling homoisoflavoinoids