Smartphone DNA or RNA Sensing Using Semisynthetic Luciferase-Based Logic Device.
Detection of specific oligonucleotide sequences is central to numerous applications, and technologies amenable to point-of-care diagnostics or end users are needed. Here, we report a technology making use of a bioluminescent readout and smartphone quantification. The sensor is a semisynthetic luciferase (H-Luc-PNA conjugate) that is turned on by a strand-displacement reaction. We demonstrated sensing of three different microRNAs (miRs), as representative cancer biomarkers, and demonstrate the possibility to integrate an AND gate to sense two sequences simultaneously.
['Chang|Dalu|D|', 'Kim|Ki Tae|KT|', 'Lindberg|Eric|E|', 'Winssinger|Nicolas|N|0000-0003-1636-7766']
D014408:Biomarkers, Tumor D015374:Biosensing Techniques D004247:DNA D008128:Logic D008156:Luciferases D008163:Luminescent Measurements D035683:MicroRNAs D000068997:Smartphone
DNA bioluminescence PNA biosensor smartphone readout