Olive oil phenolic extract regulates interleukin-8 expression by transcriptional and posttranscriptional mechanisms in Caco-2 cells.
In this study, we investigated the ability of a phenolic extract from extra virgin olive oil (OPE) to modulate the inflammatory response in intestinal epithelial cells. Undifferentiated and differentiated Caco-2 cells were challenged with LPS (50 μg/mL) or IL-1β (5 ng/mL) to mimic the early and intermediate phase of intestinal inflammation, respectively. The effects of OPE on nuclear factor-κB-driven transcription and IL-8 promoter activity were evaluated in transfection assays, coupled to p65 nuclear translocation. Modulation of IL-8 mRNA levels by OPE was measured by quantitative RT-PCR while effects on protein levels by ELISA. Specific mitogen activated protein kinases inhibitors were used to investigate mRNA stability and the involvement of related signaling pathways. OPE prevented IL-8 expression and secretion in LPS-treated Caco-2 cells. In the presence of IL-1β OPE exhibited opposing effects on IL-8 gene transcription and mRNA/protein levels. While in IL-1β-treated cells IL-8 promoter activity was inhibited by treatment with OPE, IL-8 mRNA stability was strongly enhanced, leading to increased protein expression. Inhibitors of p38 and extracellular signal-regulated kinases partly prevented OPE effect on IL-8 mRNA levels. Intestinal epithelial cells represent a direct target of the action of olive oil phenols where they regulate IL-8 expression by transcriptional and posttranscriptional mechanisms.
['Muto|Eri|E|', "Dell'Agli|Mario|M|", 'Sangiovanni|Enrico|E|', 'Mitro|Nico|N|', 'Fumagalli|Marco|M|', 'Crestani|Maurizio|M|', 'De Fabiani|Emma|E|', 'Caruso|Donatella|D|']
D018938:Caco-2 Cells D002454:Cell Differentiation D004847:Epithelial Cells D048049:Extracellular Signal-Regulated MAP Kinases D006801:Humans D007249:Inflammation D053583:Interleukin-1beta D016209:Interleukin-8 D008070:Lipopolysaccharides D016328:NF-kappa B D000069463:Olive Oil D010636:Phenols D010936:Plant Extracts D011401:Promoter Regions, Genetic D020871:RNA Stability D012333:RNA, Messenger D015398:Signal Transduction D015533:Transcriptional Activation
Phenols Intestine Inflammation IL-8 Extra-virgin olive oil