The small GTPase KlRho5 responds to oxidative stress and affects cytokinesis.
Rho5 is the yeast homolog of the human small GTPase Rac1. We characterized the genes encoding Rho5 and the subunits of its dimeric activating guanine-nucleotide-exchange factor (GEF), Dck1 and Lmo1, in the yeast Kluyveromyces lactis. Rapid translocation of the three GFP-tagged components to mitochondria upon oxidative stress and carbon starvation indicate a similar function of KlRho5 in energy metabolism and mitochondrial dynamics as described for its Saccharomyces cerevisiae homolog. Accordingly, Klrho5 deletion mutants are hyper-resistant towards hydrogen peroxide. Moreover, synthetic lethalities of rho5 deletions with key components in nutrient sensing, such as sch9 and gpr1, are not conserved in K. lactis. Instead, Klrho5 deletion mutants display morphological defects with strengthened lateral cell walls and protruding bud scars. The latter result from aberrant cytokinesis, as observed by following the budding process in vivo and by transmission electron microscopy of the bud neck region. This phenotype can be suppressed by KlCDC42G12V, which encodes a hyper-active variant. Data from live-cell fluorescence microscopy support the notion that KlRho5 interferes with the actin moiety of the contractile actomyosin ring, with consequences different from those previously reported for mutants lacking myosin.
['Musielak|Marius|M|', 'Sterk|Carolin C|CC|', 'Schubert|Felix|F|0000-0002-4225-7598', 'Meyer|Christian|C|', 'Paululat|Achim|A|0000-0002-8845-6859', 'Heinisch|Jürgen J|JJ|0000-0003-4197-4285']
D000205:Actomyosin D048749:Cytokinesis D006801:Humans D007716:Kluyveromyces D020559:Monomeric GTP-Binding Proteins D018384:Oxidative Stress D029701:Saccharomyces cerevisiae Proteins
Yeast Cell wall Mitochondrion Nutrient stress Rho-type GTPase