Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions.
Silver nanoparticles (AgNPs) have anti-cancer effect. However, whether and how these particles could inhibit the growth of acute myeloid leukemia (AML) cells is unclear. In the present study, we prepared AgNPs with various sizes and investigated their cytotoxic effect on AML cells. We found that AgNPs could inhibit the viability of AML cells including the isolates from AML patients. AgNPs caused the production of reactive oxygen species (ROS), losses of mitochondrial membrane potential (MMP), DNA damage and apoptosis. Both vitamin C (Vit C) and N-acetyl-L-cysteine (NAC) could completely reverse the generation of ROS upon AgNPs, however only NAC but not Vit C could protect the cells from losses of MMP, DNA damage and apoptosis thoroughly. Similar results were obtained when cells were treated with silver ions alone. As NAC was not only an antioxidant to scavenge ROS but also a silver ion chelator, these data supported the model that both generation of ROS and release of silver ions played critical roles in the AgNPs-induced cytotoxic effect against AML cells. Taken together, this work elucidated the cytotoxic effect of AgNPs on AML cells and their underlying mechanism and might have significant impact on AML treatment.
['Guo|Dawei|D|', 'Zhu|Lingying|L|', 'Huang|Zhihai|Z|', 'Zhou|Haixia|H|', 'Ge|Yue|Y|', 'Ma|Wenjuan|W|', 'Wu|Jie|J|', 'Zhang|Xiuyan|X|', 'Zhou|Xuefeng|X|', 'Zhang|Yu|Y|', 'Zhao|Yun|Y|', 'Gu|Ning|N|']
D000111:Acetylcysteine D000293:Adolescent D000328:Adult D000368:Aged D000970:Antineoplastic Agents D000975:Antioxidants D017209:Apoptosis D001205:Ascorbic Acid D045744:Cell Line, Tumor D004249:DNA Damage D005260:Female D018922:HL-60 Cells D006801:Humans D007477:Ions D007938:Leukemia D008297:Male D053078:Membrane Potential, Mitochondrial D053768:Metal Nanoparticles D008875:Middle Aged D017382:Reactive Oxygen Species D012834:Silver
Silver nanoparticles Reactive oxygen species Silver ions Acute myeloid leukemia Cytotoxicity