Recruitment of SH-containing peptides to lipid and biological membranes through the use of a palmitic acid functionalized with a maleimide group.
This study presents a novel and easily applicable approach to recruit sulfhydryl-containing biomolecules to membranes by using a palmitic acid which is functionalized with a maleimide group. Notably, this strategy can also be employed with preformed (biological) membranes. The applicability of the assay is demonstrated by characterizing the binding of a Rhodamine-labeled peptide to lipid and cellular membranes using methods of fluorescence spectroscopy, lifetime measurement, and microscopy. Our approach offers new possibilities for preparing biologically active liposomes and manipulating living cells.
['Haralampiev|Ivan|I|', 'Mertens|Monique|M|', 'Schwarzer|Roland|R|', 'Herrmann|Andreas|A|', 'Volkmer|Rudolf|R|', 'Wessig|Pablo|P|', 'Müller|Peter|P|']
D002462:Cell Membrane D008081:Liposomes D008264:Macrophages D008301:Maleimides D018613:Microscopy, Confocal D019308:Palmitic Acid D010455:Peptides D013050:Spectrometry, Fluorescence D013438:Sulfhydryl Compounds
peptides membranes palmitic acid maleimide liposomes palmitoylation