Studies on interaction of norbixin with DNA: multispectroscopic and in silico analysis.
The interaction of food colorant norbixin with calf thymus DNA (CTDNA) was investigated through UV-Visible spectroscopy, Fourier Transform Infrared (FTIR), Circular Dichroism (CD), Nuclear Magnetic Resonance (NMR), DNA melting studies, electrophoretic analysis, histological staining technique and molecular docking studies. The results indicated that norbixin interacted with CTDNA by partial intercalation mode. The binding constant (K) of norbixin with CTDNA was calculated to be 5.08×10(5) Mol(-1) L. FTIR and CD studies were coupled with (1)H NMR spectra revealed that norbixin intercalates partially and binds to the groove's, phosphate group, deoxyribose sugar of DNA and also induces conformational transition of B-form to A-form DNA. Agarose gel electrophoretic and histological staining technique results further prove that, norbixin specifically binds to the DNA in the cell. Moreover, molecular docking studies on the specific binding of norbixin with CTDNA have exhibited lowest conformation energy score of -3.2. Therefore, this food colorant has the ability to interact with DNA and it could emerge as a promising class of natural DNA targeted therapeutic.
['Anantharaman|Amrita|A|', 'Priya|Rajendra Rao|RR|', 'Hemachandran|Hridya|H|', 'Sivaramakrishna|Akella|A|', 'Babu|Subramanian|S|', 'Siva|Ramamoorthy|R|']
D000818:Animals D002338:Carotenoids D002417:Cattle D002942:Circular Dichroism D003198:Computer Simulation D004247:DNA D004587:Electrophoresis, Agar Gel D008968:Molecular Conformation D062105:Molecular Docking Simulation D009691:Nucleic Acid Denaturation D019697:Onions D066244:Proton Magnetic Resonance Spectroscopy D013056:Spectrophotometry, Ultraviolet D017550:Spectroscopy, Fourier Transform Infrared D013194:Staining and Labeling
Interaction Norbixin DNA In silico analysis Apocarotenoid Spectroscopic