Characterization of Guaiene Synthases from Stellera chamaejasme L. Flowers and Their Application in De novo Production of (-)-Rotundone in Yeast.
Four terpene synthases for the biosynthesis of volatile terpenoids were identified from the transcriptome of Stellera chamaejasme L. flowers, including SchTPS1, SchTPS2, SchTPS3, and SchTPS4. Their functions were characterized by synthetic biology approaches in Escherichia coli and in vitro enzymatic assays. SchTPS1, SchTPS2, and SchTPS3 are guaiene synthases, while SchTPS4 is an (E,E)-geranyl linalool synthase. Next, SchTPS1 and α-guaiene 2-oxidase VvSTO2 were co-expressed in Saccharomyces cerevisiae to reconstruct the biosynthetic pathway of (-)-rotundone, which is a unique aroma compound in fruits, vegetables, and wines. This is the first report for the construction of a (-)-rotundone-producing microbial platform.
['An|Tianyue|T|', 'Li|Linsheng|L|', 'Lin|Ying|Y|', 'Zeng|Fanqi|F|', 'Lin|Pengcheng|P|', 'Zi|Jiachen|J|http://orcid.org/0000-0002-0212-1282']
D019883:Alkyl and Aryl Transferases D052176:Azulenes D053898:Biosynthetic Pathways D035264:Flowers D015870:Gene Expression D010940:Plant Proteins D012441:Saccharomyces cerevisiae D012717:Sesquiterpenes D045789:Sesquiterpenes, Guaiane D029645:Thymelaeaceae
guaiene synthase Stellera chamaejasme L. yeast transcriptome (−)-rotundone metabolic engineering