Monoclonal antibodies and toxins--a perspective on function and isotype.
Antibody therapy remains the only effective treatment for toxin-mediated diseases. The development of hybridoma technology has allowed the isolation of monoclonal antibodies (mAbs) with high specificity and defined properties, and numerous mAbs have been purified and characterized for their protective efficacy against different toxins. This review summarizes the mAb studies for 6 toxins--Shiga toxin, pertussis toxin, anthrax toxin, ricin toxin, botulinum toxin, and Staphylococcal enterotoxin B (SEB)--and analyzes the prevalence of mAb functions and their isotypes. Here we show that most toxin-binding mAbs resulted from immunization are non-protective and that mAbs with potential therapeutic use are preferably characterized. Various common practices and caveats of protection studies are discussed, with the goal of providing insights for the design of future research on antibody-toxin interactions.
['Chow|Siu-Kei|SK|', 'Casadevall|Arturo|A|']
D000818:Animals D000911:Antibodies, Monoclonal D006801:Humans D007132:Immunoglobulin Isotypes D014118:Toxins, Biological
antibody isotype protection therapeutics animal model clearance disease enhancement in vivo neutralization vaccine