Limiting glioma development by photodynamic therapy-generated macrophage vaccine and allo-stimulation: an in vivo histological study in rats.
Immunotherapy of brain tumors involves the stimulation of an antitumor immune response. This type of therapy can be targeted specifically to tumor cells thus sparing surrounding normal brain. Due to the presence of the blood-brain barrier, the brain is relatively isolated from the systemic circulation and, as such, the initiation of significant immune responses is more limited than other types of cancers. The purpose of this study was to show that the efficacy of tumor primed antigen presenting macrophage (MaF98) vaccines can be increased by: (1) photodynamic therapy (PDT) of the priming tumor cells and (2) intracranial injection of allogeneic glioma cells directly into the tumor site. Experiments were conducted in an in vivo brain tumor development model using Fischer rats and F98 (syngeneic) and BT4C (allogeneic) glioma cells. The results showed that immunization with Ma (acting as antigen-presenting cells), primed with PDT-treated tumor cells (MaF98), significantly slowed but did not prevent the growth of F98-induced tumors in the brain. Complete suppression of tumor development was obtained via MaF98 inoculation combined with direct intracranial injection of allogeneic glioma cells. No deleterious effects were noted in any of the animals during the 14-day observation period.
['Madsen|Steen J|SJ|', 'Christie|Catherine|C|', 'Huynh|Khoi|K|', 'Peng|Qian|Q|', 'Uzal|Francisco A|FA|', 'Krasieva|Tatiana B|TB|', 'Hirschberg|Henry|H|']
D000818:Animals D001932:Brain Neoplasms D019496:Cancer Vaccines D045744:Cell Line, Tumor D005910:Glioma D006651:Histocytochemistry D007167:Immunotherapy D008264:Macrophages D008297:Male D010778:Photochemotherapy D051381:Rats
glioma macrophages photodynamic therapy allogeneic cells immunogenic apoptotic cells photodynamic therapy-macrophage vaccine