Inoculation of Lewis lung carcinoma cells enhances formalin-induced pain behavior and spinal Fos expression in mice.
The incidence of lung cancer has rapidly increased and cancer patients at a later cancer stage frequently suffer from unbearable cancer-associated pain. However, the pathophysiology of lung cancer pain has not been fully described due to a lack of appropriate animal models. This study was designed to determine the effect of Lewis lung carcinoma (LLC) cell inoculation on formalin-induced pain behavior and spinal Fos expression in C57BL/6 mice. LLC cells (1.5 × 105, 2.5 × 105, 3.0 × 105 or 5.0 × 105) were inoculated into back or peri-sciatic nerve areas. Back area inoculation was adopted to determine the effect of cancer cell circulating factors and the peri-sciatic nerve area was used to evaluate the possible effects of cancer cell contacting and circulating factors on formalin-induced pain. At postinoculation day 7, LLC cell (5.0 × 105) inoculations in both back and peri-sciatic nerve area significantly increased formalin-induced paw-licking time and spinal Fos expression over those in cell-media-inoculated (control) mice. Enhanced pain behavior and spinal Fos expression were significantly suppressed by ibuprofen pretreatment (250 mg/kg). The results of this study suggest that LLC cell circulating factors and inflammatory responses may be critical in enhancing pain sensation in the early stage of lung cancer cell inoculation.
['Choi|Jae-Gyun|JG|', 'Kim|Jae-Min|JM|', 'Kang|Dong-Wook|DW|', 'Choi|Jung-Wan|JW|', 'Park|Jin Bong|JB|', 'Ahn|Seong-Hun|SH|', 'Ryu|Yeonhee|Y|', 'Kim|Hyun-Woo|HW|']
D018712:Analgesics, Non-Narcotic D000818:Animals D000072716:Cancer Pain D018827:Carcinoma, Lewis Lung D005557:Formaldehyde D007052:Ibuprofen D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D009368:Neoplasm Transplantation D016761:Oncogene Proteins v-fos D010146:Pain D013116:Spinal Cord D066148:Spinal Cord Dorsal Horn
Lewis lung carcinoma pain Fos formalin lung neoplasms