Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.
Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing. EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium). Selected isolates produced different EPS amounts ranging from 86 to 170 mg L-1 and 26 to 105 mg L-1 when grown on M1 and M2, respectively. The use of M1 encouraged stronger bacterial growth associated with greater EPS production compared to M2. Nevertheless, the highest EPS yield (YEPS/X) was observed for strains grown on M2. When cultivated on M1, all isolates produced EPS exhibiting almost the same monosaccharide profile with mannose, glucose and arabinose being the main monomers. However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
['Joulak|Ichrak|I|', 'Finore|Ilaria|I|', 'Nicolaus|Barbara|B|', 'Leone|Luigi|L|', 'Moriello|Aniello Schiano|AS|', 'Attia|Hamadi|H|', 'Poli|Annarita|A|', 'Azabou|Samia|S|']
D055598:Chemical Phenomena D003470:Culture Media D004777:Environment D005843:Geography D020632:Halomonas D009005:Monosaccharides D010802:Phylogeny D011135:Polysaccharides, Bacterial D012462:Saline Solution, Hypertonic D014416:Tunisia
Exopolysaccharides (EPS) Monosaccharide profile Halomonas spp. Medium composition