Reference values for salivary testosterone in adolescent boys and girls determined using Isotope-Dilution Liquid-Chromatography Tandem Mass Spectrometry (ID-LC-MS/MS).
The measurement of testosterone in saliva is an attractive alternative to serum analysis due to the simple and non-invasive sample collection. In children and adolescents salivary testosterone is mainly measured to investigate whether puberty has started or not. This study aimed to establish reference values for salivary testosterone during puberty in boys and girls. We measured salivary testosterone using ID-LC-MS/MS in a cohort of 131 girls and 123 boys of whom each had salivary testosterone measured at two time points during puberty. Salivary testosterone concentrations start to increase with the start of puberty around eight years and continuously increase up to adult concentrations in the following ten years. Reference values were calculated using the Lambda-Mu-Sigma (LMS)-curve fitting method and provided per year from 8 to 26 years of age in boys and girls. These reference ranges may help clinicians and researchers to interpret salivary testosterone results in both individual patients and study subjects.
['Büttler|Rahel M|RM|', 'Peper|Jiska S|JS|', 'Crone|Eveline A|EA|', 'Lentjes|Eef G W|EGW|', 'Blankenstein|Marinus A|MA|', 'Heijboer|Annemieke C|AC|']
D000293:Adolescent D000328:Adult D002648:Child D002853:Chromatography, Liquid D005260:Female D006801:Humans D057230:Limit of Detection D008297:Male D012016:Reference Values D012463:Saliva D053719:Tandem Mass Spectrometry D013739:Testosterone D055815:Young Adult
Reference values Salivary testosterone Adolescents