Circulating microRNA 216 as a Marker for the Early Identification of Severe Acute Pancreatitis.
BACKGROUND
To study the value of circulating microRNA 216 (miR-216) as a marker for the severity of acute pancreatitis (AP) in both murine models and patients.


MATERIALS AND METHODS
Mice with AP were induced by intraperitoneal injection of 50μg/kg/hour cerulean either 7 times, sacrificed at 8, 9, 10, 11 or 12 hours after the first injection, or 12 times, sacrificed at 24 hours after the first injection. Plasma samples and data from patients with AP were obtained from a prospective cohort. Quantitative reverse transcription polymerase chain reaction was used to determine the miR-216a and miR-216b level.


RESULTS
The upregulation of miR-216a and miR-216b in the serum of mice was induced by cerulean injection in both the 7- and 12-injection groups (P < 0.05). The downregulation of miR-216a in pancreatic tissues of mice with AP was detected (P < 0.05), but no difference was observed in pancreatic miR-216b levels among any of the groups (all P > 0.05). The serum miR-216a level was positively correlated with pancreatic histopathology severity scores, and was negatively correlated with pancreatic miR-216a (r = -0.483, P = 0.009). The plasma miR-216a level was significantly upregulated in patients with severe AP (SAP) compared with patients with mild AP (MAP) or moderate severe AP (MSAP) (SAP versus MAP, P = 0.04; SAP versus MSAP, P = 0.00), but no difference was seen between patients with MAP and those with MSAP (P = 0.73).


CONCLUSIONS
Circulating miR-216a might be a potential biomarker for the early identification of SAP.
['Zhang|Xiao-Xin|XX|', 'Deng|Li-Hui|LH|', 'Chen|Wei-Wei|WW|', 'Shi|Na|N|', 'Jin|Tao|T|', 'Lin|Zi-Qi|ZQ|', 'Ma|Yun|Y|', 'Jiang|Kun|K|', 'Yang|Xiao-Nan|XN|', 'Xia|Qing|Q|']
D000208:Acute Disease D000328:Adult D000681:Amylases D000818:Animals D015415:Biomarkers D005260:Female D006801:Humans D008297:Male D008807:Mice, Inbred BALB C D035683:MicroRNAs D008875:Middle Aged D010179:Pancreas D010195:Pancreatitis
microRNA 216 Severe acute pancreatitis Acute pancreatitis miR-216a Biomarker