Triple Activity of Lamivudine Releasing Sulfonated Polymers against HIV-1.
In this article a library of polymeric therapeutic agents against the human immunodeficiency virus (HIV) is presented. The library of statistical copolymers of varied molar mass was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization. The synthesized polymers comprise pendent hydroxyl and sulfonated side chains as well as the reverse transcriptase prodrug lamivudine (3TC) attached via a disulfide self-immolative linker. The glutathione mediated release of 3TC is demonstrated as well as the antiviral efficacy against HIV entry and polymerase activity. Although a high degree of polymer sulfonation is required for effective HIV entry inhibition, polymers with approximately ∼50% sulfonated monomer demonstrated potent kinase independent reverse transcriptase inhibition. In addition, the sulfonated polymers demonstrate activity against DNA-DNA polymerase, which suggests that these polymers may exhibit activity against a broad spectrum of viruses. In summary, the polymers described provide a triple-active arsenal against HIV with extracellular activity via entry inhibition and intracellular activity by kinase-dependent lamivudine-based and kinase-independent sulfonated polymer based inhibition. Since these sulfonated copolymers are easily formulated into gels, we envision them to be particularly suited for topical application to prevent the mucosal transmission of viruses, particularly HIV.
['Danial|Maarten|M|', 'Andersen|Anna H F|AH|', 'Zuwala|Kaja|K|', 'Cosson|Steffen|S|', 'Riber|Camilla Frich|CF|', 'Smith|Anton A A|AA|', 'Tolstrup|Martin|M|', 'Moad|Graeme|G|', 'Zelikin|Alexander N|AN|', 'Postma|Almar|A|']
D019380:Anti-HIV Agents D015497:HIV-1 D019259:Lamivudine D009682:Magnetic Resonance Spectroscopy D011108:Polymers D018894:Reverse Transcriptase Inhibitors D014779:Virus Replication
HIV-1 RAFT self-immolative linker antiviral drug delivery inhibitor