Transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae.
Porcine respiratory disease complex (PRDC) is of great concern economically, for swine producers worldwide. Co-infections with porcine reproductive and respiratory syndrome virus (PRRSV) and Mycoplasma hyopneumoniae (Mhp) are considered the major causative agents of PRDC, and responsible for mass mortality in pigs. Nevertheless, the molecular mechanisms underlying the host factors involved in pathogenesis and persistent infection have not been clearly established because of a lack of information regarding host responses following co-infection. In the current study, high throughput cDNA microarray assays were employed to evaluate host responses of porcine alveolar macrophages (PAM) to co-infection with highly pathogenic PRRSV (HP-PRRSV) and Mhp. A total of 2152 and 1760 genes were identified as being differentially expressed between the control group and PRRSV+Mhp co-infected group at 6 and 15 h post infection, respectively. The DE genes were involved in many vital functional classes, including inflammatory response, immune response, apoptosis, defense response, signal transduction. The pathway analysis demonstrated that the most significant pathways were associated with chemokine signaling pathway, cytokine, TLR, RLR and NLR signaling pathways and Jak-STAT signaling pathway. STRING analysis demonstrated that IL-1β is an integral gene in co-infections with PRRSV and Mhp. The present study is the first to document the response of PAMs to co-infection with HP-PRRSV and Mhp. The observed gene expression profile could help with the screening of potential host agents for reducing the prevalence of co-infections, and to further develop our understanding of the molecular pathogenesis associated with PRRSV and Mhp co-infection in pigs.
['Li|Bin|B|', 'Du|Luping|L|', 'Xu|Xiangwei|X|', 'Sun|Bing|B|', 'Yu|Zhengyu|Z|', 'Feng|Zhixin|Z|', 'Liu|Maojun|M|', 'Wei|Yanna|Y|', 'Wang|Haiyan|H|', 'Shao|Guoqing|G|', 'He|Kongwang|K|']
D000818:Animals D016000:Cluster Analysis D060085:Coinfection D019295:Computational Biology D020869:Gene Expression Profiling D054884:Host-Pathogen Interactions D053583:Interleukin-1beta D016676:Macrophages, Alveolar D058977:Molecular Sequence Annotation D045705:Mycoplasma hyopneumoniae D045729:Pneumonia of Swine, Mycoplasmal D019318:Porcine Reproductive and Respiratory Syndrome D019316:Porcine respiratory and reproductive syndrome virus D015203:Reproducibility of Results D015398:Signal Transduction D013552:Swine D059467:Transcriptome
Co-infection Mycoplasma hyopneumoniae PRRSV Microarray analysis