Broncho-Vaxom® (OM-85 BV) soluble components stimulate sinonasal innate immunity.
BACKGROUND
Broncho-Vaxom® (OM-85 BV) is an extract of infectious respiratory bacteria that is used as an immunostimulant outside of the United States for the prevention and treatment of bronchitis and rhinosinusitis. Prior studies have shown that use of OM-85 BV is associated with reduction in frequency of respiratory infection and decreased duration of antibiotic usage. However, the effects of OM-85 BV on respiratory mucosal innate immunity are unknown.


METHODS
Human sinonasal epithelial cells were grown at an air-liquid interface (ALI). Ciliary beat frequency (CBF) and nitric oxide (NO) production in response to stimulation with OM-85 BV was measured in vitro. Pharmacologic inhibitors of bitter taste receptor (T2R) signaling were used to determine if this pathway was taste-receptor-mediated.


RESULTS
Apical application of OM-85 BV resulted in an NO-mediated increase in CBF (p < 0.05) and increased NO production (p < 0.0001) when compared to saline-stimulated control cultures. ALI pretreatment with taste receptor pathway inhibitors blocked OM-85 BV-induced increases in NO.


CONCLUSION
OM-85 BV has ciliostimulatory and immunogenic properties that may be partially responsible for its observed efficacy as a respiratory therapeutic. These responses were NO-dependent and consistent with T2R activation. Further work is necessary to elucidate specific component-receptor signaling relationships.
['Triantafillou|Vasiliki|V|', 'Workman|Alan D|AD|', 'Patel|Neil N|NN|0000-0003-1365-9130', 'Maina|Ivy W|IW|0000-0003-4426-2718', 'Tong|Charles C L|CCL|0000-0002-4471-5134', 'Kuan|Edward C|EC|0000-0003-3475-0718', 'Kennedy|David W|DW|', 'Palmer|James N|JN|', 'Adappa|Nithin D|ND|', 'Waizel-Haiat|Salomon|S|', 'Cohen|Noam A|NA|']
D000276:Adjuvants, Immunologic D002457:Cell Extracts D002478:Cells, Cultured D002923:Cilia D004847:Epithelial Cells D006801:Humans D007113:Immunity, Innate D009297:Nasal Mucosa D009569:Nitric Oxide
innate immunity rhinosinusitis therapeutics ciliary motility immunotherapy sinusitis