Association of exposure to benzene and smoking with oxidative damage to nucleic acids by means of biological monitoring of general population volunteers.
In this study, the validation of liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) isotopic dilution method for the determination of benzene and nicotine metabolites in urine was carried out. Limit of detection are 0.026 μg/L for S-phenylmercapturic acid (SPMA), 0.55 μg/L for t,t-muconic acid (t,t-MA), and 12.41 μg/L for the cotinine, and the relative combined uncertainty was also calculated. The study involves 446 healthy volunteer residents since at least 10 years in an area of central Italy. SPMA resulted to be strongly correlated with cotinine (p = 0.75), its concentration in smokers (93) being about ten times than in non/ex-smokers (197/156), while the t,t-MA of smokers is about twice the non/ex-smokers value. A cutoff value for the definition of smoker is set at 100 μg/g creat. Oxidative stress was studied in smokers and non- and ex-smokers by means of the determination of the biomarkers 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 8-Oxo-7,8-dihydroguanosine (8-oxoGuo), and 8-Oxo-7,8-dihydroguanine (8-oxoGua): no significant differences were found between smokers and non/ex-smokers, but when subjects are classified according to the cotinine cutoff value, a correlation in smokers' urinary 8-oxodGuo is found with SPMA and cotinine (p = 0.60 and p = 0.57). Results were confirmed by chemometric analysis that also identified the experimental variables most contributing the discrimination as cotinine and t,t-MA.
['Tranfo|G|G|http://orcid.org/0000-0001-8973-6046', 'Pigini|D|D|', 'Paci|E|E|', 'Marini|F|F|', 'Bonanni|R C|RC|']
D000111:Acetylcysteine D000328:Adult D001554:Benzene D015415:Biomarkers D004781:Environmental Exposure D004784:Environmental Monitoring D004785:Environmental Pollutants D005260:Female D006801:Humans D007558:Italy D008297:Male D008875:Middle Aged D009696:Nucleic Acids D018384:Oxidative Stress D012907:Smoking D013011:Sorbic Acid D053719:Tandem Mass Spectrometry D014838:Volunteers
HPLC-MS/MS S-Phenylmercapturic acid t,t-Muconic acid 8-Oxo-7,8-dihydroguanosine 8-Oxo-7,8-dihydroguanine 8-Oxo-7,8-dihydro-2′-deoxyguanosine Biomonitoring Urinary cotinine