A new method for hydroxyl radical detection by chemiluminescence of flue-cured tobacco extracts.
Chemiluminescence (CL) reactions usually take place in a severely restricted pH regime, thereby confining their application in media at different pH. In this paper, the CL behavior of tobacco-methanol extract (TME) was explored. Surprisingly, TME exhibited CL behavior upon mixing with Fe2+/H2O2, HRP/H2O2 and gold nanoparticles/H2O2 oxidation systems, i.e., in acidic, neutral and alkaline solution respectively. Addition of different reactive oxygen species scavengers verified that the hydroxyl radical (OH) triggers TME CL reactions. Then, the CL behavior of TME was applied to determine OH in tetrachloro-1,4-benzoquinone (TCBQ)/H2O2 system in acidic, neutral and alkaline solutions. CL intensity correlated most strongly (R2 = 0.99) with TCBQ concentration, which was used as a means to indirectly denote OH concentration. This implies that OH could be determined by a TME CL method in a semi-quantitative way regardless of pH value. Therefore, the TME CL method may be a promising method for OH determination in various OH-generating systems.
['Wang|Dabin|D|', 'Yu|Weisong|W|', 'Pang|Xueli|X|', 'Cao|Jianmin|J|', 'Qiu|Jun|J|', 'Kong|Fanyu|F|']
D006863:Hydrogen-Ion Concentration D017665:Hydroxyl Radical D008163:Luminescent Measurements D000432:Methanol D010084:Oxidation-Reduction D010936:Plant Extracts D013057:Spectrum Analysis D014026:Tobacco
Hydroxyl radical detection Chemiluminescence pH Tobacco-methanol extract