The antitumor activity of umbelliferone in human renal cell carcinoma via regulation of the p110γ catalytic subunit of PI3Kγ.
Umbelliferone exhibits extensive pharmacological activity, including anti-immunomodulatory, anti-inflammatory and antigenotoxicity activities. However, its antitumor properties still remain unclear in human renal cell carcinoma (RCC) cells. Our results have revealed that treatment of human RCC cells (786-O, OS-RC-2, and ACHN) with umbelliferone reduced cell proliferation in a concentration-dependent manner and induced dose-dependent apoptotic events. In addition, cell cycle analysis determined that umbelliferone treatment induced cell cycle arrest in the G1 phase in a dose-dependent manner. Furthermore, western blotting analysis showed a dose-dependent decrease in Ki67, MCM2, Bcl-2, CDK2, CyclinE1, CDK4, and CyclinD1 and a dose-dependent increase in Bax in RCC cells cultured with umbelliferone. Similarly, umbelliferone exhibited a dose-dependent reduction of p110γ when using western blotting analyses. Taken together, these results provide an insight into the pharmacology regarding the potential application of umbelliferone, which contributes to cell death by decreasing p110γ protein expression.
['Wang|Xue|X|', 'Huang|Shuaishuai|S|', 'Xin|Xia|X|', 'Ren|Yu|Y|', 'Weng|Guobin|G|', 'Wang|Ping|P|']
D000970:Antineoplastic Agents D017209:Apoptosis D002292:Carcinoma, Renal Cell D020134:Catalytic Domain D059447:Cell Cycle Checkpoints D016923:Cell Death D045744:Cell Line, Tumor D049109:Cell Proliferation D058544:Class Ib Phosphatidylinositol 3-Kinase D016193:G1 Phase D006801:Humans D007680:Kidney Neoplasms D014468:Umbelliferones
umbelliferone renal cell carcinoma p110γ anti-cancer activity