Effect of Lignocaine Concentration on Human Fibroblasts Growth in Eyes Undergoing Trabeculectomy: An in vitro Study.
Purpose
To evaluate the effect of lignocaine on growth and apoptosis indication of primary human Tenon's capsule fibroblast (HTFs) in an in vitro model.


Patients and Methods
Tenon's capsule tissue obtained from patients undergoing trabeculectomy were grown in cell culture medium. The effect of different concentrations of lignocaine (0.5, 1.0, 1.5, and 2%) on the morphology and growth of the fibroblasts was studied using microscopy, cell viability, and proliferation assay, and apoptosis was detected using the FITC Annexin V Apoptosis Kit.


Results
Morphological changes similar to those of apoptotic cells, including cytoplasmic vacuolation, shrinkage, and rounding were visualized in the cells treated with concentrations greater than 1.0% (i.e., 1.5, 2.0%). Though proliferation inhibition was found with all four concentrations (0.5-2.0%), the viability of cells decreased from 1.0% lignocaine.


Conclusion
0.5% lignocaine prevents proliferation of fibroblasts without causing apoptosis in vitro.
['Janani|Madhuravasal Krishnan|MK|', 'Jaichandran|Venkatakrishnan|V|', 'Madhavan|Hajib Narahari Rao|HNR|', 'Vijaya|Lingam|L|', 'George|Ronnie Jacob|RJ|', 'Ambastha|Priyam Shrikant|PS|', 'Manu|Abhijeet|A|', 'Malathi|Jambulingam|J|']
Lignocaine Trabeculectomy Tenon's fibroblast