Evaluation of Leishmania (Leishmania) infantum excreted-secreted antigens for detection of canine leishmaniasis.
The efficacy of tests with L. (L.) infantum excreted-secreted antigens (ESA) to detect canine leishmaniasis (CanL) was evaluated using immunoblotting (ESA-blot), ELISA (ESA-ELISA) and ELISA with alkaline extract from promastigotes (PAE). Of one hundred fifty-five domestic dogs tested, 100 were suspected of CanL, 23 had other diseases and 32 were healthy. Sera from the dogs suspected of CanL were tested by immunohistochemistry (IHC), and 54% were confirmed to be infected by L. (L.) infantum (38 symptomatic and 16 asymptomatic). Of these, 100% were positive by ESA-blot, ESA-ELISA and PAE-ELISA. In the ESA-blot their sera recognized polypeptides in the 26.5-31.5kDa region. Of the 46% of dogs with negative IHC, 44-53% tested positive in all three tests irrespective of clinical status. The twenty-three dogs with other diseases were negative by ESA-blot, but sera from 9% and 26% of them reacted with ESA-ELISA and PAE-ELISA, respectively. The 32 healthy dogs were negative in all the tests. ESA-blot showed good correlation with IHC in the detection of CanL and a high specificity index.
['Pinedo-Cancino|Viviana|V|', 'Laurenti|Márcia Dalastra|MD|', 'Kesper|Norival|N|', 'Umezawa|Eufrosina Setsu|ES|']
D000818:Animals D000953:Antigens, Protozoan D004283:Dog Diseases D004285:Dogs D004797:Enzyme-Linked Immunosorbent Assay D005260:Female D015151:Immunoblotting D018314:Leishmania infantum D007898:Leishmaniasis, Visceral D008297:Male D012680:Sensitivity and Specificity
Canine leishmaniasis Immunoblotting ELISA Excreted antigens Leishmania infantum Secreted antigens