Effect of P-glycoprotein regulation on cadmium exhaustion from Crassostrea gigas.
P-glycoprotein (P-gp) is a molecular pump, responsible for extruding xenobiotics. The aim of the study was to demonstrate the role of P-glycoprotein (P-gp) in cadmium (Cd) exhaustion. The activity of P-gp was regulated in Crassostrea gigas, which was previously exposed to Cd by using rifampicin (inducer) and verapamil (inhibitor), respectively. Comparing with Crassostrea gigas depurated in natural seawater, Cd content increased significantly from 14.28 mg/kg dw to 17.49 mg/kg dw accompanied by a changed metallothionein level from 9.84 μg/g fw to 10.67 μg/g fw after 25 μg/L verapamil treatment, while Cd content after 25 μg/L rifampicin treatment reduced to 12.21 mg/kg dw. Moreover, after treatment with rifampicin and verapamil, beneficial metal elements, fats, and proteins were maintained, and the tissue-dependent difference was found in the variation of antioxidant defenses and oxidative damage in Crassostrea gigas. In brief, the study provided new evidence on possibility of Cd removal by inducing P-glycoprotein.
['Yang|Chao|C|', 'Liu|Qingkang|Q|', 'Li|Guangzhen|G|', 'Meng|Xianghong|X|', 'Liu|Bingjie|B|']
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 D000818:Animals D000975:Antioxidants D002104:Cadmium D049896:Crassostrea D008658:Inactivation, Metabolic D008668:Metallothionein D012293:Rifampin D012623:Seawater D014018:Tissue Distribution D014700:Verapamil D014874:Water Pollutants, Chemical
P-glycoprotein Cadmium Exhaustion Crassostrea gigas Rifampicin induce