Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure.
Pardosa pseudoannulata is one of the most common wandering spiders in agricultural fields and a potentially good bioindicator for heavy metal contamination. However, little is known about the mechanisms by which spiders respond to heavy metals at the molecular level. In the present study, high-throughput transcriptome sequencing was employed to characterize the de novo transcriptome of the spiders and to identify differentially expressed genes (DEGs) after cadmium exposure. We obtained 60,489 assembled unigenes, 18,773 of which were annotated in the public databases. A total of 2939 and 2491 DEGs were detected between the libraries of two Cd-treated groups and the control. Functional enrichment analysis revealed that metabolism processes and digestive system function were predominately enriched in response to Cd stress. At the cellular and molecular levels, significantly enriched pathways in lysosomes and phagosomes as well as replication, recombination and repair demonstrated that oxidative damage resulted from Cd exposure. Based on the selected DEGs, certain critical genes involved in defence and detoxification were analysed. These results may elucidate the molecular mechanisms underlying spiders' responses to heavy metal stress.
['Li|Chang-Chun|CC|', 'Wang|Yong|Y|', 'Li|Guo-Yuan|GY|', 'Yun|Yue-Li|YL|', 'Dai|Yu-Jun|YJ|', 'Chen|Jian|J|', 'Peng|Yu|Y|']
D000818:Animals D002104:Cadmium D020869:Gene Expression Profiling D063990:Gene Ontology D059014:High-Throughput Nucleotide Sequencing D008658:Inactivation, Metabolic D058977:Molecular Sequence Annotation D012333:RNA, Messenger D060888:Real-Time Polymerase Chain Reaction D015203:Reproducibility of Results D013112:Spiders D059467:Transcriptome
transcriptome cadmium P. pseudoannulata RT-qPCR