Human catalase gene polymorphism (CAT C-262T) and risk of male infertility.
Infertility is the failure of a couple to engender after endeavouring at least one full year of unprotected intercourse. It has been reported that reactive oxygen species contributed to pathogenesis of various disease. To inactivate ROS cells biosynthesise several antioxidant enzymes, one of them is catalase which contributes H2 O2 to H2 O and O2 . This study set out to delineate the association of catalase C-262T polymorphism with idiopathic male infertility. The study included 195 men with idiopathic infertility and 190 healthy volunteers. Genomic DNA was extracted from peripheral blood leucocytes. Genotype and allele frequencies were determined in patients and controls using allele-specific PCR (AS-PCR). The prevalence of genotype frequencies of the CAT CC/CT/TT was 31.79%, 65.12% and 3.07%, respectively, in infertile subjects, as against 24.73%, 55.26% and 20%, respectively, in healthy volunteers. Statistical analysis has emerged significant difference from the comparison of either genotype (P < 0.05). Taking into accounts of results, the catalase C-262T polymorphism indicates that CAT-262T/T genotype confers less susceptibility to male infertility. Further studies with larger numbers of patients are required for further evaluation and confirmation of our finding.
['Sabouhi|S|S|', 'Salehi|Z|Z|', 'Bahadori|M H|MH|', 'Mahdavi|M|M|']
D000328:Adult D053627:Asthenozoospermia D016022:Case-Control Studies D002374:Catalase D005787:Gene Frequency D020022:Genetic Predisposition to Disease D006801:Humans D007248:Infertility, Male D008297:Male D009845:Oligospermia D011110:Polymorphism, Genetic
Catalase polymorphism idiopathic male infertility oxidative stress