Exploration of age-related mitochondrial dysfunction and the anti-aging effects of resveratrol in zebrafish retina.
It is currently believed that aging is closely linked with mitochondrial dysfunction, and that resveratrol exhibits anti-aging and neuroprotective effects by improving mitochondrial function, even though the mechanisms are not well defined. This study explored mitochondrial quality (mitochondrial DNA integrity and copy number), mitochondrial function (fusion/fission, mitophagy/autophagy), antioxidant system and activity of the Akt/mTOR and Ampk/Sirt1/Pgc1α pathways, and inflammation in aging zebrafish retinas to identify the probable mechanisms of resveratrol's anti-aging and neuroprotective effects. mtDNA integrity, mtDNA copy number, mitochondrial fusion regulators, mitophagy, and antioxidant-related genes were all decreased whereas Akt/mTOR activity and inflammation was increased upon aging in zebrafish retinas. Resveratrol was shown to not only increase mitochondrial quality and function, but also to suppress Akt/mTOR activity in zebrafish retinas. These results support the notion that mitochondrial dysfunction and increased Akt/mTOR activity are major players in age-related retinal neuropathy in zebrafish, and demonstrate a trend towards mitochondrial fragmentation in the aging retina. Importantly, resveratrol promoted mitochondrial function, up-regulating Ampk/Sirt1/Pgc1α, and down-regulated Akt/mTOR pathway activity in zebrafish retinas, suggesting that it may be able to prevent age-related oculopathy.
['Wang|Ning|N|', 'Luo|Zhiwen|Z|', 'Jin|Ming|M|', 'Sheng|Weiwei|W|', 'Wang|Han-Tsing|HT|', 'Long|Xinyi|X|', 'Wu|Yue|Y|', 'Hu|Piaopiao|P|', 'Xu|Hong|H|', 'Zhang|Xu|X|']
D000375:Aging D000818:Animals D000975:Antioxidants D004272:DNA, Mitochondrial D004353:Drug Evaluation, Preclinical D063154:Mitochondrial Dynamics D063306:Mitophagy D000077185:Resveratrol D012164:Retinal Diseases D015027:Zebrafish
mitochondrial dysfunction resveratrol retina mitophagy mTOR aging zebrafish