The Effects of Trypsin Inhibitor on Insulin Secretion Using Rat Pancreas in an Organ Bath.
BACKGROUND/AIM
We developed an experimental method to reproduce insulin secretion from isolated rat pancreas preparations using an organ bath system. However, secretion of trypsin, another pancreatic enzyme, interferes with insulin production in such systems. We aimed to ascertain the minimum trypsin inhibitor (TI), dose for obtaining a sustained, stable rate of insulin secretion.


MATERIALS AND METHODS
The action of TI (1-10 μg/ml) on pancreatic preparations of male Wistar-Imamichi rats in organ bath experiments was assessed by measuring insulin, amylase, and trypsin activity.


RESULTS
The level of insulin outflow remained steady in the TI-treated samples, in contrast to that in the untreated control, where insulin secretion decreased over time. The level of amylase outflow did not change significantly. Trypsin activity was significantly lower in the TI-treated samples than in the control.


CONCLUSION
Even low concentrations of TI can maintain insulin secretion by inhibiting trypsin activity in organ bath experiments.
['Morita|Asuka|A|', 'Ouchi|Motoshi|M|', 'Satoh|Keitaro|K|', 'Kobayashi|Shunsuke|S|', 'Terada|Misao|M|', 'Wakashin|Hidefumi|H|', 'Kon|Hiroe|H|', 'Hayashi|Keitaro|K|', 'Anzai|Naohiko|N|', 'Shimizu|Akira|A|', 'Sugihara|Hitoshi|H|', 'Oba|Kenzo|K|', 'Fujita|Tomoe|T|']
D000681:Amylases D000818:Animals D007328:Insulin D000078790:Insulin Secretion D008297:Male D010179:Pancreas D051381:Rats D017208:Rats, Wistar D014361:Trypsin Inhibitors
trypsin inhibitor Insulin pancreas organ bath amylase