Practical two-photon-absorption cross sections and spectra of eosin and hematoxylin.
We experimentally investigate the two-photon absorption cross sections and spectra of eosin and hematoxylin for applications in nonlinear microscopy. The experiments are carried out on pure samples of the two dyes in DI-water solvent with different concentrations, in the typical range employed in standard staining procedures. Nonlinear fluorescence is excited by a line-shaped beam emitted by a Ti:Sapphire mode-locked laser in the wavelength range from 740 to 880 nm and is detected through a microscope setup. The two-photon absorption spectral response is systematically analyzed and discussed. Finally, the staining is applied on biological tissue samples, which are imaged by two-photon microscopy. Our results show that the employed dyes are fully suitable for applications in nonlinear imaging.
['Parravicini|Jacopo|J|0000-0002-0548-3385', 'Tomaselli|Alessandra|A|', 'Hasani|Elton|E|', 'Tomassini|Daniela|D|', 'Manfredi|Norberto|N|', 'Tartara|Luca|L|']
D004801:Eosine Yellowish-(YS) D006416:Hematoxylin D008853:Microscopy D017785:Photons D013194:Staining and Labeling
eosin hematoxylin two-photon absorption microscopy dyes nonlinear fluorescence