Circulating fetal and total cell-free DNA, and sHLA-G in black South African women with gestational hypertension and pre-eclampsia.
OBJECTIVE
Quantification of circulating fetal and total cell-free DNA (cfDNA) and soluble human leucocyte antigen (HLAG) in gestational hypertension and pre-eclampsia.


METHODS
Serum cfDNA were quantified in controls, pre-eclamptics, and gestational hypertensive patients using real-time qPCR. Soluble HLAG was measured by enzyme-linked immune-sorbent assay.


RESULTS
Serum fetal and total cfDNA levels were higher in pre-eclampsia compared with the controls and gestational hypertensives (p < 0.001), more so in severe compared with mild-to-moderate pre-eclampsia (p < 0.05). Soluble HLAG levels were lower in pre-eclamptics than controls and gestational hypertension (p < 0.05).


CONCLUSION
Circulating fetal and total cfDNA were increased, while soluble HLAG was decreased in pre-eclampsia.
['Eche|Simeon|S|', 'Mackraj|Irene|I|', 'Moodley|Jagidesa|J|']
D000328:Adult D044383:Blacks D000073888:Cell-Free Nucleic Acids D005260:Female D059951:HLA-G Antigens D006801:Humans D046110:Hypertension, Pregnancy-Induced D011225:Pre-Eclampsia D011247:Pregnancy D013019:South Africa D055815:Young Adult
total cell-free DNA gestational hypertension pre-eclampsia soluble human leucocyte antigen Fetal cell-free DNA