Of Molecules and Mechanisms.
Without question, molecular biology drives modern neuroscience. The past 50 years has been nothing short of revolutionary as key findings have moved the field from correlation toward causation. Most obvious are the discoveries and strategies that have been used to build tools for visualizing circuits, measuring activity, and regulating behavior. Less flashy, but arguably as important are the myriad investigations uncovering the actions of single molecules, macromolecular structures, and integrated machines that serve as the basis for constructing cellular and signaling pathways identified in wide-scale gene or RNA studies and for feeding data into informational networks used in systems biology. This review follows the pathways that were opened in neuroscience by major discoveries and set the stage for the next 50 years.
['Benson|Deanna L|DL|0000-0001-7037-1147']
D000818:Animals D064113:CRISPR-Cas Systems D005089:Exocytosis D005786:Gene Expression Regulation D018014:Gene Transfer Techniques D017930:Genes, Reporter D049673:History, 20th Century D049674:History, 21st Century D006801:Humans D017403:In Situ Hybridization D008853:Microscopy D008967:Molecular Biology D009419:Nerve Tissue Proteins D009488:Neurosciences D054731:PDZ Domains D016133:Polymerase Chain Reaction D015202:Protein Engineering D012313:RNA D011994:Recombinant Proteins D017422:Sequence Analysis, DNA
RNA CRISPR-Cas9 PCR SNARE and synapse composition nanoscale imaging transfection and transduction