Expression of HLA-DQA1 and HLA-DQB1 genes in B lymphocytes, monocytes and whole blood.
Differential expression of HLA-DQA1 and HLA-DQB1 gene alleles was analysed in three different cell populations isolated from peripheral blood-B lymphocytes, monocytes and whole-blood cells. Interallelic differences in mRNA levels were observed: DQA1*03 alleles were among the most expressed in all cell types, whereas DQA1*05 alleles were least expressed in whole blood and monocytes and among the most expressed in B cells. For DQB1 gene, DQB1*06 group of alleles were the most expressed, and DQB1*02 group the least expressed within all cell populations examined. In comparison with the rest alleles, DQB1*06 and DQB1*05:02 alleles have higher expression in monocytes than in B cells, professional antigen-presenting cells. Cell type-specific regulation of expression was observed as well, with higher and more balanced expression of alleles in B lymphocytes compared to monocytes.
['Zajacova|M|M|', 'Kotrbova-Kozak|A|A|', 'Cerna|M|M|http://orcid.org/0000-0002-1208-5588']
D000328:Adult D000368:Aged D000483:Alleles D001402:B-Lymphocytes D005260:Female D015870:Gene Expression D005787:Gene Frequency D059848:HLA-DQ alpha-Chains D059866:HLA-DQ beta-Chains D006239:Haplotypes D064368:Healthy Volunteers D006801:Humans D008297:Male D008875:Middle Aged D009000:Monocytes D009928:Organ Specificity D012333:RNA, Messenger D055815:Young Adult
HLA-DQA1 HLA-DQB1 HLA class II antigen presentation gene expression genetic polymorphism