Carbazate modified dextrans as scavengers for carbonylated proteins.
A series of biocompatible and non- toxic polysaccharide molecules have been successfully fabricated and explored their potential application for scavenging the carbonyl species in vitro. These macromolecules were dextrans with different hydrazide substitution ratios determined by TNBS assay, NMR and FTIR characterization. The colorimetric assay had demonstrated that these macromolecules could effectively scavenge acrolein, oxidized bovine serum albumin (BSA) in buffer solutions as well as carbonyl proteins from serum. The scavengers could achieve twice more scavenging effects for modified dextrans with high molecular weight (Mw = 100,000) than those of low ones (Mw = 40,000) with the same substitution ratio. Protein gel electrophoresis confirmed that the formation of the complex between carbonyls and modified dextrans resulted in appearance of slower bands. It also revealed that such macromolecules could protect cultured cells against the toxicity of acrolein or its derivatives. The proposed macromolecules indicated a very promising capability as scavengers for oxidative stress plus its derivatives without side effects.
['Zhou|Bo|B|', 'Gao|Ming|M|', 'Feng|Xianjing|X|', 'Huang|Lanli|L|', 'Huang|Quanxin|Q|', 'Kootala|Sujit|S|', 'Larsson|Tobias E|TE|', 'Zheng|Li|L|', 'Bowden|Tim|T|']
D000818:Animals D002417:Cattle D003911:Dextrans D016166:Free Radical Scavengers D006834:Hydrazines D015394:Molecular Structure D050050:Protein Carbonylation D012710:Serum Albumin, Bovine
Protein gel electrophoresis Oxidative stress Carbonyl scavengers Human blood serum Hydrazide-carbonyl click chemistry Protein carbonyl groups