Emulsion droplet crystallinity attenuates early in vitro digestive lipolysis and beta-carotene bioaccessibility.
The impacts of lipid crystallinity on in vitro digestive lipolysis and bioaccessibility of encapsulated (0.1 wt%) beta-carotene (BC) were investigated for a 15 wt% cocoa butter emulsion prepared as crystalline (i.e. solid emulsions, SE & SE-BC) or undercooled (liquid emulsions, LE & LE-BC) droplets at 25 °C. Particle size distributions (D4,3 ∼0.7 μm), morphology (spherical), polymorphism (beta-V), thermal behavior (peak melting ∼30 °C), zeta potential (∼-44 mV) and BC degradation under accelerated lighting conditions were similarly extensive. Following exposure to simulated gastric conditions, duodenal hydrolysis and BC bioaccessibility were lower for SE-BC up to 2 h (P < 0.05). Ultimately, samples with both solid and liquid droplets were hydrolyzed extensively and BC bioaccessibility did not differ (P > 0.05). Therefore, for compositionally equivalent emulsions, lipid droplet solid state delayed digestive lipolysis and bioactive solubilization. These results help to clarify the role of lipid physical state on dietary lipid digestion and bioactive release.
['Hart|Samantha M|SM|', 'Lin|Xinjie Lois|XL|', 'Thilakarathna|Surangi H|SH|', 'Wright|Amanda J|AJ|']
D004041:Dietary Fats D004063:Digestion D004386:Duodenum D004655:Emulsions D006801:Humans D066292:Lipid Droplets D008066:Lipolysis D010316:Particle Size D011136:Polysorbates D014280:Triglycerides D019207:beta Carotene
Emulsion In vitro digestion Bioaccessibility Lipids Physical properties