Long non-coding RNA MIAT acts as a biomarker in diabetic retinopathy by absorbing miR-29b and regulating cell apoptosis.
Diabetic retinopathy (DR) is a complication of diabetes mellitus (DM) and is the leading cause of vision loss globally. However, the pathogenic mechanism and clinical therapy still needs further improvement. The biologic significance of myocardial infarction associated transcript (MIAT) in DR remains unknown. Here, we aim to explore the mechanism between MIAT and DR, which is essential for RD. Streptozotocin (STZ) was used to induce DM mice and high glucose was used to stimulate cells. ChIP was used to detect the binding activity between nuclear factor κB (NF-κB) and the promoter of the MIAT gene, luciferase activity assay was used to detect the target-specific selectivity between miR-29b and MIAT. The expressions of MIAT and p-p65 were increased in STZ-induced DM mice and high glucose stimulated rat retinal Müller cells (rMC-1) cells. ChIP results revealed that high glucose promoted the binding activity between NF-κB and MIAT, while Bay11-7082 acted as an inhibitor for NF-κB that suppressed the binding activity. miR-29b controled MIAT to regulate its expression and MIAT overexpression suppressed miR-29b, but promoted Sp1. High glucose stimulation increased the cell apoptosis and decreased the cell activity, while MIAT suppression reversed the effect induced by high glucose, however, miR-29b knockdown reversed the effects induced by MIAT suppression. Our results provided evidence that the mechanism of cell apoptosis in DR might be associated with the regulation of MIAT, however, miR-29b acted as a biomarker that was regulated by MIAT and further regulated cell apoptosis in DR.
['Zhang|Jiayu|J|', 'Chen|Maochong|M|', 'Chen|Jiawei|J|', 'Lin|Sisi|S|', 'Cai|Daqiu|D|', 'Chen|Chengwei|C|', 'Chen|Zhenguo|Z|']
D000818:Animals D017209:Apoptosis D002460:Cell Line D003921:Diabetes Mellitus, Experimental D003930:Diabetic Retinopathy D005786:Gene Expression Regulation D005819:Genetic Markers D008297:Male D051379:Mice D035683:MicroRNAs D062085:RNA, Long Noncoding D051381:Rats D015854:Up-Regulation
MIAT diabetic retinopathy miR-29b cell apoptosis high glucose