A novel CNS-homing peptide for targeting neuroinflammatory lesions in experimental autoimmune encephalomyelitis.
Using phage peptide library screening, we identified peptide-encoding phages that selectively home to the inflamed central nervous system (CNS) of mice with experimental autoimmune encephalomyelitis (EAE), a model of human multiple sclerosis (MS). A phage peptide display library encoding cyclic 9-amino-acid random peptides was first screened ex-vivo for binding to the CNS tissue of EAE mice, followed by in vivo screening in the diseased mice. Phage insert sequences that were present at a higher frequency in the CNS of EAE mice than in the normal (control) mice were identified by DNA sequencing. One of the phages selected in this manner, denoted as MS-1, was shown to selectively recognize CNS tissue in EAE mice. Individually cloned phages with this insert preferentially homed to EAE CNS after an intravenous injection. Similarly, systemically-administered fluorescence-labeled synthetic MS-1 peptide showed selective accumulation in the spinal cord of EAE mice. We suggest that peptide MS-1 might be useful for targeted drug delivery to CNS in EAE/MS.
['Acharya|Bodhraj|B|', 'Meka|Rakeshchandra R|RR|', 'Venkatesha|Shivaprasad H|SH|', 'Lees|Jason R|JR|', 'Teesalu|Tambet|T|', 'Moudgil|Kamal D|KD|']
D000818:Animals D001921:Brain D002490:Central Nervous System D019295:Computational Biology D004681:Encephalomyelitis, Autoimmune, Experimental D059014:High-Throughput Nucleotide Sequencing D007249:Inflammation D051379:Mice D008810:Mice, Inbred C57BL D019151:Peptide Library D010455:Peptides D013116:Spinal Cord
CNS EAE Multiple sclerosis Homing peptide Phage library Probes Targeted delivery