New phenolic glycosides from Anemone chinensis Bunge and their antioxidant activity.
ABATRACTNine compounds, five phenolic glycosides (1, 2, 4-6), three phenylpropanoids (7-9), and a furanone glycoside (3), were isolated from aqueous soluble extract of the dried roots of Anemone chinensis Bunge. The structures of new compounds (1-4) were elucidated by comprehensive spectroscopic data analysis as well as chemical evidence. Pulsatillanin A (1) demonstrated significant antioxidant effects through scavenging free radical in DPPH assay, and relieved the oxidative stress in LPS-induced RAW 264.7 cells by reducing ROS production, enhancing antioxidant enzyme SOD activity, replenishing depleted GSH in a dose-dependent manner. Western blot analysis revealed that 1 showed antioxidant activity via activating Nrf2 signaling pathway.
['Zhang|Zeng-Guang|ZG|', 'Li|Yuan-Yuan|YY|', 'Lin|Bin|B|', 'Guan|Pei-Pei|PP|', 'Mu|Yu|Y|', 'Qiao|Wen-Jun|WJ|', 'Zhang|Jing-Sheng|JS|', 'Huang|Xue-Shi|XS|', 'Han|Li|L|']
phenolic glycosides Anemone chinensis Bunge antioxidant activity Nrf2