Comparison of gene expression of Toll-like receptors and cytokines between Piau and Commercial line (Landrace×Large White crossbred) pigs vaccinated against Pasteurella multocida type D.
We aimed to compare Toll-like receptors (TLR) and cytokines expression in local Piau breed and a Commercial line (Landrace×Large White crossbred) pigs in response to vaccination against Pasteurella multocida type D. Seronegative gilts for Pasteurella multocida type D and Mycoplasma hyopneumoniae were used, from which peripheral blood mononuclear cells (PBMC) were collected in four time points (T0, T1, T2 and T3; before and after each vaccination dose). For bronchoalveolar lavage fluid cells (BALF), we set groups of vaccinated and unvaccinated animals for both genetic groups. Gene expression was evaluated on PBMC and BALF. In PBMC, when we analyzed time points within breeds, significant differences in expression for TLRs and cytokines, except TGFβ, were observed for Commercial animals. For the Piau pigs, only TGFβ showed differential expression. Comparing the expression among genetic groups, the Commercial pigs showed higher expression for TLRs after first vaccination dose, while for IL2, IL6, IL12 and IL13, higher expression was also observed in T3 and IL8 and IL10, in T1 and T3. Still comparing the breeds, the crossbred animals showed higher expression for TNFα in T1 and T2, while for TGFβ only in T2. For gene expression in BALF, vaccinated Commercial pigs showed higher expression of TLR6, TLR10, IL6, IL8, IL10, TNFα and TGFβ genes than vaccinated Piau pigs. The Commercial line pigs showed higher sensitivity to vaccination, while in local Piau breed lower responsiveness, which may partly explain genetic variability in immune response and will let us better understand the tolerance/susceptibility for pasteurellosis.
['Sousa|Katiene Régia Silva|KRS|', 'Ribeiro|André Mauric Frossard|AMF|', 'Dantas|Waleska de Melo Ferreira|WMF|', 'Oliveira|Leandro Licursi de|LL|', 'Gasparino|Eliane|E|', 'Guimarães|Simone Eliza Facioni|SEF|']
D000818:Animals D016207:Cytokines D005260:Female D015870:Gene Expression D007963:Leukocytes, Mononuclear D010326:Pasteurella Infections D016979:Pasteurella multocida D012222:Rhinitis, Atrophic D034421:Sus scrofa D013552:Swine D013553:Swine Diseases D051193:Toll-Like Receptors D014611:Vaccination
Cytokines Immune response Pasteurellosis Disease resistance Swine