Identification of essential hypertension biomarkers in human urine by non-targeted metabolomics based on UPLC-Q-TOF/MS.
BACKGROUND
In recent years, using metabolomics technology to study hypertension has made some progress. However, in actual clinical studies, there are few studies on hypertension related metabonomics with human urine as samples. In this study, the urine samples of patients with essential hypertension (EH) were studied by comparing with healthy people to explore the changes of urine metabolites between hypertensive patients and healthy people in order to find potential biomarkers and metabolic pathways.


METHODS
An ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) technology was used to analyze the urine metabolites of 75 cases of essential hypertension group (EH) and 75 cases of healthy control group (HC).


RESULTS
According to the PLS-DA pattern recognition analysis, substances with significant differences (P < .05) between the EH group and the HC group were screened out, including 10 potential biomarkers such as L-methionine. The metabolic pathways involved were amino acid metabolism, fatty acid metabolism steroid hormone, biosynthesis and oxidative stress.


CONCLUSION
The non-targeted metabolomics based on UPLC-Q-TOF/MS technology can effectively identify the differential metabolites of potential biomarkers in the urine of essential hypertensive patients and provide a theoretical basis for the treatment of clinical hypertension.
['Zhao|Huan|H|', 'Liu|Yijia|Y|', 'Li|Zhu|Z|', 'Song|Yanqi|Y|', 'Cai|Xuemeng|X|', 'Liu|Yuechen|Y|', 'Zhang|Tianpu|T|', 'Yang|Liu|L|', 'Li|Lin|L|', 'Gao|Shan|S|', 'Li|Yubo|Y|', 'Yu|Chunquan|C|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D015415:Biomarkers D016022:Case-Control Studies D002851:Chromatography, High Pressure Liquid D000075222:Essential Hypertension D006801:Humans D013058:Mass Spectrometry D055432:Metabolomics D008875:Middle Aged D012984:Software D055815:Young Adult
Essential hypertension Urine Metabolomics UPLC-Q-TOF/MS Markers