Hb Phnom Penh Showing Falsely High or Reasonable HbA1c Values Depending on the Type of High-performance Liquid Chromatography System.
We herein report a 50-year-old Chinese woman with Hb Phnom Penh (α117Phe-Ile-α118Thr) showing high or reasonable HbA1c values depending on the type of high-performance liquid chromatography (HPLC) system. A high HbA1c value of 7.5% (HPLC assay: G9) and a reasonable HbA1c value of 5.2% (assay unknown) were observed. Therefore, the patient was refereed to our hospital; the oral glucose tolerance test showed normal glucose tolerance. The HbA1c values measured by an enzymatic assay, immunoassay, and affinity assay, as well as most HPLC assays were within the reference range, whereas those measured by the Tosoh HPLC systems were high.
['Hara|Kenji|K|', 'Koga|Masafumi|M|', 'Shinozaki|Hiroyuki|H|', 'Yamauchi|Mototaka|M|', 'Suzuki|Tatsuhiko|T|', 'Naruse|Rika|R|', 'Tsuchiya|Takafumi|T|', 'Takebayashi|Kohzo|K|', 'Inukai|Toshihiko|T|', 'Hashimoto|Koshi|K|']
D002851:Chromatography, High Pressure Liquid D005260:Female D005951:Glucose Tolerance Test D006442:Glycated Hemoglobin A D006455:Hemoglobins, Abnormal D006801:Humans D007118:Immunoassay D008875:Middle Aged D012680:Sensitivity and Specificity
Hb Phnom Penh HbA1c high performance liquid chromatography variant hemoglobin