Sphingosine inhibits the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) activity.
The increase in the intracellular Ca(2+) concentration ([Ca(2+)]i) is the key variable for many different processes, ranging from regulation of cell proliferation to apoptosis. In this work we demonstrated that the sphingolipid sphingosine (Sph) increases the [Ca(2+)]i by inhibiting the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA), in a similar manner to thapsigargin (Tg), a specific inhibitor of this Ca(2+) pump. The results showed that addition of sphingosine produced a release of Ca(2+) from the endoplasmic reticulum followed by a Ca(2+) entrance from the outside mileu. The results presented in this work support that this sphingolipid could control the activity of the SERCA, and hence sphingosine may participate in the regulation of [Ca(2+)]I in mammalian cells.
['Benaim|Gustavo|G|', 'Pimentel|Adriana A|AA|', 'Felibertt|Pimali|P|', 'Mayora|Adriana|A|', 'Colman|Laura|L|', 'Sojo|Felipe|F|', 'Rojas|Héctor|H|', 'De Sanctis|Juan B|JB|']
D002118:Calcium D045744:Cell Line, Tumor D004721:Endoplasmic Reticulum D004789:Enzyme Activation D006801:Humans D009369:Neoplasms D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases D013110:Sphingosine
Sphingosine SERCA Sphingolipid Ca(+2) Sarco(endo)plasmic reticulum Ca(+2)-ATPase