A tumorigenic cell line derived from a hamster cholangiocarcinoma associated with Opisthorchis felineus liver fluke infection.
AIMS
The food-born trematode Opisthorchis felineus colonizes bile ducts of the liver of fish-eating mammals including humans. There is growing evidence that this liver fluke is a risk factor for cholangiocarcinoma (CCA). Cancer cell lines are necessary for drug screening and for identifying protein markers of CCA. The aim was to establish a cell line derived from cholangiocarcinoma associated with opisthorchiasis felinea.


MAIN METHODS
Allotransplantation, immunohistochemistry, karyotype analysis, cell culture techniques, immunocytochemistry and real-time PCR.


KEY FINDINGS
Here we repot the establishment of first CCA cell line, CCA-OF, from a primary tumor of an experimental CCA in Syrian hamsters treated with low doses of dimethyl nitrosamine and associated with O. felineus infection. The cell line was found to be allotransplantable. Expression of epithelial and mesenchymal markers (cytokeratin 7, glycosyltransferase exostosin 1, Ca2+-dependent phospholipid-binding protein annexin A1 and vimentin) was demonstrated by immunostaining of the primary tumors, CCA-OF cells, and allotransplants. CCA-OF cells were found to express presumed CCA biomarkers previously detected in both human and experimental tumors associated with the liver fluke infection. The cells were diploid-like (2n = 42-46) with complex chromosomal rearrangements and have morphological features of epithelial-like cells. The usefulness of the CCA-OF cell model for antitumor activity testing was demonstrated by an analysis of effects of resveratrol treatment. It was shown that resveratrol treatment inhibited the proliferation and the migration ability of CCA-OF cells.


SIGNIFICANCE
Thus, the allotransplantable CCA-OF cell line can be used in studies on helminth-associated cholangiocarcinogenesis and for the testing of antitumor drugs.
['Mordvinov|Viatcheslav A|VA|', 'Minkova|Galina A|GA|', 'Kovner|Anna V|AV|', 'Ponomarev|Dmitriy V|DV|', 'Lvova|Maria N|MN|', 'Zaparina|Oxana|O|', 'Romanenko|Svetlana A|SA|', 'Shilov|Alexander G|AG|', 'Pakharukova|Maria Y|MY|']
D000818:Animals D001650:Bile Duct Neoplasms D001653:Bile Ducts, Intrahepatic D063646:Carcinogenesis D002460:Cell Line D018281:Cholangiocarcinoma D006224:Cricetinae D004847:Epithelial Cells D008099:Liver D009889:Opisthorchiasis
Cell line Hamster Cholangiocarcinoma Opisthorchis felineus Experimental model