Biocidal effects of volatile organic compounds produced by the myxobacterium Corrallococcus sp. EGB against fungal phytopathogens.
Myxobacteria have excellent biocontrol activity against various phytopathogens due to their rich spectrum of secondary metabolites and active predatory characteristics. In this study, the mycelial growth of Fusarium oxysporum f. sp. cucumerinum (FOC) was found to be significantly inhibited by volatile compounds (VOCs) produced by Corallococcus sp. EGB. A total of 32 compounds were identified among the VOCs produced by strain EGB, of which isooctanol exhibited the highest antifungal activity, with dosages of 3.75 and 4.0 μL/plate being sufficient to suppress FOC and Penicillum digitatum, respectively. Isooctanol was found to damage the cell wall and cell membranes of FOC and P. digitatum. Apoptosis-like cell death of FOC and P. digitatum induced by isooctanol was observed subsequently due to the accumulation of reactive oxygen species (ROS). The transcription level of genes related to cell wall integrity (CWI) pathway and redox reactions were significantly upregulated by 15- to 40-fold, indicating the stress caused by isooctanol. Postharvest storage experiments showed that the disease severity of post-harvest oranges infected with P. digitatum could be significantly reduced by isooctanol at 114.2 μL/L.
['Ye|Xianfeng|X|', 'Chen|Yang|Y|', 'Ma|Shiyun|S|', 'Yuan|Tian|T|', 'Wu|Yaxuan|Y|', 'Li|Yingxuan|Y|', 'Zhao|Yuqiang|Y|', 'Chen|Shuying|S|', 'Zhang|Yiwen|Y|', 'Li|Liuyan|L|', 'Li|Zhoukun|Z|', 'Huang|Yan|Y|', 'Cao|Hui|H|', 'Cui|Zhongli|Z|']
D000935:Antifungal Agents D002473:Cell Wall D032084:Citrus sinensis D061353:Food Storage D005670:Fusarium D050296:Microbial Viability D025282:Mycelium D009231:Myxococcales D000442:Octanols D010935:Plant Diseases D017382:Reactive Oxygen Species D055549:Volatile Organic Compounds
Fusarium oxysporum f. sp. cucumerinum Volatile compounds Penicillum digitatum Cell wall integrity Postharvest storage Apoptosis-like death