Frequency dependent impedimetric cytotoxic evaluation of anticancer drug on breast cancer cell.
The present work reports the impedance characteristics of MCF-7 cell lines treated with anticancer drug ZD6474 to evaluate the cytotoxic effect on cellular electrical behaviour using miniature impedance sensors. Four types of impedance sensing devices with different electrode geometries are fabricated by microfabrication technology. The frequency response characteristics of drug treated cells are studied to evaluate cytotoxic effect of ZD6474 and also to assess the frequency dependent sensitivity variation with electrode area. A significant variation in magnitude of measured impedance data is obtained for drug treated samples above 10 µM dose indicating prominent effect of ZD6474 which results in suppression of cell proliferation and induction of apoptosis process. The results obtained by impedimetric method are correlated well with conventional in vitro assays such as flow cytometry, cell viability assays and microscopic imaging. Finally an empirical relation between cell impedance, electrode area and drug dose is established from impedance data which exhibits a negative correlation between drug doses and impedance of cancer cells.
['Pradhan|Rangadhar|R|', 'Rajput|Shashi|S|', 'Mandal|Mahitosh|M|', 'Mitra|Analava|A|', 'Das|Soumen|S|']
D000970:Antineoplastic Agents D017209:Apoptosis D001681:Biological Assay D015374:Biosensing Techniques D001943:Breast Neoplasms D049109:Cell Proliferation D058266:Dielectric Spectroscopy D004305:Dose-Response Relationship, Drug D004353:Drug Evaluation, Preclinical D004867:Equipment Design D019544:Equipment Failure Analysis D005260:Female D006801:Humans D061986:MCF-7 Cells D010880:Piperidines D011799:Quinazolines D015203:Reproducibility of Results D012680:Sensitivity and Specificity D016896:Treatment Outcome
Cytotoxicity MCF-7 ZD6474 Microfabrication Bioimpedance