Next-generation sequencing of Chinese stage IV lung cancer patients reveals an association between EGFR mutation status and survival outcome.
Large-scale genomic characterization of non-small cell lung cancer (NSCLC) has revealed several putative oncogenic driver mutations that may constitute druggable therapeutic targets. However, there are little data to suggest that such gene alterations have clinical relevance. Over 12 consecutive months, tumor biopsy samples from 80 patients with stage IV NSCLC were analyzed for mutations in selected exons of 508 cancer-related genes using next-generation sequencing. From 85 specimens referred for genomic characterization, 80 (94%) specimens were successfully genotyped, and all had identifiable somatic alterations. Epidermal growth factor receptor (EGFR) and TP53 genes contained the highest frequency of observed mutations (65% and 40%, respectively) in the stage IV NSCLC cases. Notably, patients with EGFR mutations showed a significantly shorter survival time compared with patients expressing wild-type EGFR (p = 0.0053). Moreover, of the 32 patients harboring EGFR mutations, EGFR-L858R mutant patients showed a significantly shorter survival time compared with patients with other EGFR mutations (p = 0.036). In conclusion, tumors from stage IV NSCLC patients harbor characteristic gene alterations, of which EGFR L858R in particular appears to be a poor prognostic factor for overall survival.
['Li|F|F|', 'Du|X|X|', 'Zhang|H|H|', 'Ju|T|T|', 'Chen|C|C|', 'Qu|Q|Q|', 'Zhang|X|X|', 'Qi|L|L|', 'Lizée|G|G|']
D000328:Adult D000368:Aged D044466:Asians D014408:Biomarkers, Tumor D018572:Disease-Free Survival D066246:ErbB Receptors D005260:Female D059014:High-Throughput Nucleotide Sequencing D006801:Humans D008175:Lung Neoplasms D008297:Male D008875:Middle Aged D009154:Mutation D009367:Neoplasm Staging D011379:Prognosis
next-generation sequencing survival non-small cell lung cancer (NSCLC) epidermal growth factor receptor (EGFR) gene mutation prognosis