MicroRNA-205-5p targets the HOXD9-Snail1 axis to inhibit triple negative breast cancer cell proliferation and chemoresistance.
MicroRNA-205 (miR-205) is believed to be related to the progress of tumors. HOXD9 has been proved to be expressed abnormally in several kinds of cancers. However, the role of miR-205 and HOXD9 in breast cancer remains unclear. The biological role of miR-205 in breast cancer cell proliferation and chemoresistance was investigated. The expression of miR-205 in clinical tissues and breast cancer cell lines were analyzed using quantitative real-time PCR test (qRT-PCR). Overexpression and knockdown models of miR-205 were established to study cell proliferation and chemotherapy-resistant. Moreover, the potential relationships between miR-205 and HOXD9/Snail1 were measured using qRT-PCR, western blot, and chemotherapy-resistant study. miR-205 was lowly expressed in breast cancer tissues and cell lines. Overexpression of miR-205 could inhibit cell proliferation and chemotherapy-resistance. Moreover, we proved that miR-205 could target the HOXD9-Snail1 axis to suppress triple negative breast cancer cell proliferation and chemoresistance. The activation of Snail1 gene by HOXD9 was also proved in this study. The present study may provide a novel insight for the therapeutic strategies of breast cancer through targeting miR-205/HOXD9/Snail1.
['Lin|Li-Fen|LF|', 'Li|Yi-Ting|YT|', 'Han|Hui|H|', 'Lin|Shun-Guo|SG|']
D000970:Antineoplastic Agents D045744:Cell Line, Tumor D049109:Cell Proliferation D002470:Cell Survival D002945:Cisplatin D004317:Doxorubicin D019008:Drug Resistance, Neoplasm D005260:Female D055785:Gene Knockdown Techniques D018398:Homeodomain Proteins D006801:Humans D035683:MicroRNAs D009363:Neoplasm Proteins D017239:Paclitaxel D000071250:Snail Family Transcription Factors D064726:Triple Negative Breast Neoplasms
breast cancer EMT chemotherapy resistance microRNA