Disodium cromoglycate treatment reduces TH2 immune response and immunohistopathological features in a murine model of Eosinophilic Esophagitis.
Eosinophilic esophagitis (EoE) is an emergent chronic disease of the esophagus. The immunopathological process in EoE is characterized by Th2 immune response and prominent eosinophilic influx, in response to common food allergens. The classical treatment consists of allergen elimination diet and systemic/topical corticosteroid therapy. Nevertheless, patients do not always comply to treatment, and the prolonged corticosteroid therapy can cause side effects, therefore, there is an immediate need for new therapeutic approach for EoE. Disodium cromoglicate (DSCG) is a substance broadly used in allergic asthma treatment, and a well-known mast cell activation stabilizer. However, its effect in EoE have not been evaluated yet. This study aimed to assess the effects of DSCG treatment in an EoE experimental model. Male Balb/C mice were subcutaneously sensitized for five days with OVA, and subsequently orally OVA-challenged, DSCG administration was performed between the OVA-challenges. DSCG treatment not only reduced eosinophilic and mast cell influx, as well as reduced fibrosis. In addition, tslp, GATA3, IL-5, FoxP3 and IL-10 mRNA expression were reduced in esophageal mucosa, associated with lower Th2 (CD3+CD4+GATA3+IL4+) and B cells (CD19+CD40+) number in peripheral lymphoid organs. In conclusion, the data demonstrate DSCG treatment was effective in reducing mast cell activation and Th2 immune response, important immunopathological EoE features. Therefore, the use of DSCG as an EoE treatment can be considered a promising therapeutic approach to treat this disease.
['Silva|Flávia Márcia de Castro E|FMCE|', 'de Oliveira|Erick Esteves|EE|', 'Ambrósio|Marcilene Gomes Evangelista|MGE|', 'Ayupe|Marina Caçador|MC|', 'de Souza|Viviane Passos|VP|', 'Menegati|Laura Machado|LM|', 'Reis|Daniele Ribeiro de Lima|DRL|', 'Machado|Marco Antonio|MA|', 'Macedo|Gilson Costa|GC|', 'Ferreira|Ana Paula|AP|']
D000818:Animals D001853:Bone Marrow D004205:Cromolyn Sodium D004195:Disease Models, Animal D057765:Eosinophilic Esophagitis D004804:Eosinophils D000071041:Esophageal Mucosa D005355:Fibrosis D007109:Immunity D008221:Lymphoid Tissue D008297:Male D000082142:Mast Cell Stabilizers D008407:Mast Cells D051379:Mice D008807:Mice, Inbred BALB C D010047:Ovalbumin D018418:Th2 Cells
Disodium cromoglycate Immune response Eosinophilic esophagitis Allergy Inflammation