Vitamin C Activates Osteoblastogenesis and Inhibits Osteoclastogenesis via Wnt/β-Catenin/ATF4 Signaling Pathways.
This study evaluated the effects of vitamin C on osteogenic differentiation and osteoclast formation, and the effects of vitamin C concentration on bone microstructure in ovariectomized (OVX) Wistar rats. Micro-computed tomography analysis revealed the recovery of bone mineral density and bone separation in OVX rats treated with vitamin C. Histomorphometrical analysis revealed improvements in the number of osteoblasts, osteoclasts, and osteocytes; the osteoblast and osteoclast surface per bone surface; and bone volume in vitamin C-treated OVX rats. The vitamin C-treated group additionally displayed an increase in the expression of osteoblast differentiation genes, including bone morphogenetic protein-2, small mothers against decapentaplegic 1/5/8, runt-related transcription factor 2, osteocalcin, and type I collagen. Vitamin C reduced the expression of osteoclast differentiation genes, such as receptor activator of nuclear factor kappa-B, receptor activator of nuclear factor kappa-B ligand, tartrate-resistant acid phosphatase, and cathepsin K. This study is the first to show that vitamin C can inhibit osteoporosis by promoting osteoblast formation and blocking osteoclastogenesis through the activation of wingless-type MMTV integration site family/β-catenin/activating transcription factor 4 signaling, which is achieved through the serine/threonine kinase and mitogen-activated protein kinase signaling pathways. Therefore, our results suggest that vitamin C improves bone regeneration.
['Choi|Hyeon Kyeong|HK|', 'Kim|Gyeong-Ji|GJ|', 'Yoo|Han-Seok|HS|', 'Song|Da Hye|DH|', 'Chung|Kang-Hyun|KH|', 'Lee|Kwon-Jai|KJ|', 'Koo|Young Tae|YT|', 'An|Jeung Hee|JH|']
D051701:Activating Transcription Factor 4 D000821:Animal Feed D000818:Animals D001205:Ascorbic Acid D015519:Bone Density D004032:Diet D005260:Female D005786:Gene Expression Regulation D010006:Osteoblasts D010010:Osteoclasts D010024:Osteoporosis D010052:Ovariectomy D051381:Rats D017207:Rats, Sprague-Dawley D015398:Signal Transduction D051153:Wnt Proteins D051176:beta Catenin
Vitamin C osteoclasts osteoblasts osteoporosis ovariectomized rats