Spatiotemporal image correlation analysis of blood flow in branched vessel networks of zebrafish embryos.
Ramification of blood circulation is relevant in a number of physiological and pathological conditions. The oxygen exchange occurs largely in the capillary bed, and the cancer progression is closely linked to the angiogenesis around the tumor mass. Optical microscopy has made impressive improvements in in vivo imaging and dynamic studies based on correlation analysis of time stacks of images. Here, we develop and test advanced methods that allow mapping the flow fields in branched vessel networks at the resolution of 10 to 20  μm. The methods, based on the application of spatiotemporal image correlation spectroscopy and its extension to cross-correlation analysis, are applied here to the case of early stage embryos of zebrafish.
['Ceffa|Nicolo G|NG|', 'Cesana|Ilaria|I|', 'Collini|Maddalena|M|', "D'Alfonso|Laura|L|", 'Carra|Silvia|S|', 'Cotelli|Franco|F|', 'Sironi|Laura|L|', 'Chirico|Giuseppe|G|']
D000818:Animals D001808:Blood Vessels D002196:Capillaries D003198:Computer Simulation D018450:Disease Progression D006439:Hemodynamics D007091:Image Processing, Computer-Assisted D021621:Imaging, Three-Dimensional D008833:Microcirculation D008853:Microscopy D015233:Models, Statistical D009024:Morphogenesis D010100:Oxygen D062211:Spatio-Temporal Analysis D013053:Spectrophotometry D015027:Zebrafish
image correlation hemodynamics single plane illumination microscopy