Characterization of the response to low pH of Lactobacillus casei ΔRR12, a mutant strain with low D-alanylation activity and sensitivity to low pH.
AIMS
To identify the differences that account for the acid sensitivity of Lactobacillus casei ΔRR12. RR12 controls the expression of the dlt operon, and its inactivation leads to a diminished teichoic acid D-alanylation activity. To this end, a comparison of its response of ΔRR12 to low pH with the parental strain Lact. casei BL23 was carried out.


METHODS AND RESULTS
The ability to induce an acid tolerance response (ATR), fatty acid (FA) composition and proteome changes induced in both strains in response to acid were investigated. Results obtained showed that both strains induce a growth-phase-dependent ATR. However, significant differences in the content of FAs and membrane-associated proteins were detected.


CONCLUSIONS
The greater abundance of cytoplasmic proteins in the membrane fraction of the mutant strain ΔRR12 suggests an increased permeability of the cell membrane in this strain.


SIGNIFICANCE AND IMPACT OF THE STUDY
The analysis of the response to low pH of strain ΔRR12 indicated that the inactivation of TCS12 affected the content of FAs and proteins associated to the cell envelope. Increased abundance of cytoplasmic proteins suggested that low alanylation of teichoic acids affected the permeability of the cell membrane and possibly accounts for the acid sensitivity of strain ΔRR12.
['Revilla-Guarinos|A|A|', 'Alcántara|C|C|', 'Rozès|N|N|', 'Voigt|B|B|', 'Zúñiga|M|M|']
D001426:Bacterial Proteins D002462:Cell Membrane D005227:Fatty Acids D006863:Hydrogen-Ion Concentration D007780:Lactobacillus casei D008565:Membrane Proteins D009154:Mutation D020543:Proteome D013682:Teichoic Acids
Lactobacillus casei acid sensitivity acid tolerance response fatty acids D-alanylation of teichoic acids