Targeted Intracellular Controlled Drug Delivery and Tumor Therapy through in Situ Forming Ag Nanogates on Mesoporous Silica Nanocontainers.
Targeting nanocontainers to the pathological zone and controlling release of their cargoes, in particular delivery of anticancer drugs to specific tumor cells in a targeted and controlled manner, remain the key challenges in drug delivery. This paper reports the development of a traceable and tumor-targeted intracellular drug release nanocontainer. The nanocontainer is obtained by in situ growth of silver nanoparticles (AgNPs) on the surfaces of mesoporous silica nanospheres (MSNs) using a DNA-templated process. Additionally, drug release from the nanopores is achieved by selective glutathione (GSH)-triggered dismantle of the AgNPs, and the concurrent fluorescence change allows real-time monitoring of drug release efficacy and facile visualization of in vivo delivery events. After being functionalized with sgc8 aptamer on the outer shell of the AgNPs, the targeted nanocontainers are delivered into acute lymphoblastic leukemia cells by aptamer-mediated recognition and endocytosis. Moreover, the GSH-responsive process presents an improvement in the cell-specific drug release and chemotherapeutic inhibition of tumor growth.
['Liu|Changhui|C|', 'Zheng|Jing|J|', 'Deng|Li|L|', 'Ma|Cheng|C|', 'Li|Jishan|J|', 'Li|Yinhui|Y|', 'Yang|Sheng|S|', 'Yang|Jinfeng|J|', 'Wang|Jing|J|', 'Yang|Ronghua|R|']
D052157:Aptamers, Nucleotide D004317:Doxorubicin D016503:Drug Delivery Systems D006367:HeLa Cells D006801:Humans D053768:Metal Nanoparticles D054118:Nanospheres D049329:Nanostructures D016062:Porosity D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma D012822:Silicon Dioxide D012834:Silver
drug delivery glutathione DNA mesoporous silica nanoparticle silver sphere