Reduction of Ischemia Reperfusion-Related Brain Hemorrhage by Stachybotrys Microspora Triprenyl Phenol-7 in Mice With Antioxidant Effects.
BACKGROUND
Stachybotrys microspora triprenyl phenol-7 (SMTP-7) has both thrombolytic and anti-inflammatory effects, but its neuroprotective effects on cerebral ischemia are still unclear. The present study assessed the antioxidative and neurovascular unit (NVU) protective effects of SMTP-7 using transient middle cerebral artery occlusion (tMCAO) mice.


METHODS
After 60 minutes tMCAO, 0.9% NaCl, tissue-type plasminogen activator (tPA), SMTP-7 or tPA + SMTP-7 was intravenously administrated through subclavian vein just before the reperfusion, and these mice were examined at 24 hours after reperfusion. We histologically assessed the hemorrhage and expressive changes of antioxidative markers in brains.


RESULTS
SMTP-7 treatment showed a similar antithrombotic effect to tPA, but significantly decreased the hemorrhage volumes and the number of 4-HNE, 3-NT and 8-OHdG positive cells, meanwhile, ameliorated the decrease of collagen IV in the ischemic brains. However, tPA + SMTP-7 treatment did not decrease hemorrhage volumes nor showed NVU protective effect.


CONCLUSIONS
The present study suggested that SMTP-7 provided therapeutic benefits for ischemic stroke through antioxidative and NVU protective effects unlike tPA alone or tPA + SMTP-7.
['Huang|Yong|Y|', 'Ohta|Yasuyuki|Y|', 'Shang|Jingwei|J|', 'Li|Xianghong|X|', 'Liu|Xia|X|', 'Shi|Xiaowen|X|', 'Feng|Tian|T|', 'Yamashita|Toru|T|', 'Sato|Kota|K|', 'Takemoto|Mami|M|', 'Hishikawa|Nozomi|N|', 'Suzuki|Eriko|E|', 'Hasumi|Keiji|K|', 'Abe|Koji|K|']
D000818:Animals D000975:Antioxidants D001578:Benzopyrans D001921:Brain D004195:Disease Models, Animal D005343:Fibrinolytic Agents D020244:Infarction, Middle Cerebral Artery D020300:Intracranial Hemorrhages D008297:Male D008813:Mice, Inbred ICR D018696:Neuroprotective Agents D011760:Pyrrolidinones D015427:Reperfusion Injury D010959:Tissue Plasminogen Activator
Antioxidative SMTP-7 neurovascular unit Tpa ischemic stroke