Membrane Poration Mechanisms at the Cell-Nanostructure Interface.
3D vertical nanostructures have become one of the most significant methods for interfacing cells and the nanoscale and for accessing significant intracellular functionalities such as membrane potential. As this intracellular access can be induced by means of diverse cellular membrane poration mechanisms, it is important to investigate in detail the cell condition after membrane rupture for assessing the real effects of the poration techniques on the biological environment. Indeed, differences of the membrane dynamics and reshaping have not been observed yet when the membrane-nanostructure system is locally perturbed by, for instance, diverse membrane breakage events. In this work, new insights are provided into the membrane dynamics in case of two different poration approaches, optoacoustic- and electro-poration, both mediated by the same 3D nanostructures. The experimental results offer a detailed overview on the different poration processes in terms of electrical recordings and membrane conformation.
['Dipalo|Michele|M|0000-0002-1823-8231', 'Caprettini|Valeria|V|', 'Bruno|Giulia|G|', 'Caliendo|Fabio|F|', 'Garma|Leonardo D|LD|', 'Melle|Giovanni|G|', 'Dukhinova|Marina|M|', 'Siciliano|Velia|V|', 'Santoro|Francesca|F|', 'De Angelis|Francesco|F|']
D000818:Animals D002460:Cell Line D002462:Cell Membrane D004594:Electrophysiology D018274:Electroporation D004867:Equipment Design D051379:Mice D008839:Microelectrodes D049329:Nanostructures D061088:Photoacoustic Techniques
electrophysiology electroporation membrane deformation plasmonic optoporation scanning electron microscopy