DNA cleavage induced by [Cu(L)x(NO3)2] (L=2,2'-dipyridylamine, 2,2'-bipyridine, dipicolylamine, x=1 or 2): Effect of the ligand structure.
The efficiency of [Cu(2,2'-bipyridine)2(NO3)]NO3, [Cu(2,2'-dipyridylamine)2(NO3)2], and [Cu(dipicolylamine)2(NO3)2] complexes (complex 1, 2 and 3, respectively) in oxidative DNA cleavage was examined by electrophoresis and linear dichroism (LD). Among the three Cu complexes, complex 1 showed the highest efficiency in super-coiled DNA (scDNA) cleavage in electrophoresis. The presence of tiron, a superoxide radical scavenger, suppressed the reaction almost completely. The LD signal at 260 nm decreased gradually as the time passed. The decrease in LD magnitude was explained best by the sum of the two single exponential curves. This suggests that the cleavage reaction involves two first order kinetic processes; an increase in flexibility due to scission of one of the strands and a shortening in the DNA stem due to cut of both strands of double stranded DNA (dsDNA). In agreement with the electrophoresis data, complex 1 exhibited the highest efficiency with the superoxide radical found to be the essential reactive oxygen species. The order of efficiency in both scDNA and dsDNA was as follows: complex 1>complex 2>complex 3. The electrochemical properties alone were insufficient to explain the observed efficiencies, even though reduction of the central Cu ion is essential for the oxidative DNA cleavage. This highlights the importance of an ability to ligate the molecular oxygen (or hydrogen peroxide) to the central Cu ion to produce the superoxide radical, in addition to the reduction of Cu ion, in oxidative DNA cleavage.
['Kwon|Ji Hye|JH|', 'Park|Hee-Jin|HJ|', 'Chitrapriya|Nataraj|N|', 'Cho|Tae-Sub|TS|', 'Kim|Soojin|S|', 'Kim|Jinheung|J|', 'Hwang|In Hong|IH|', 'Kim|Cheal|C|', 'Kim|Seog K|SK|']
D003300:Copper D004247:DNA D055664:Electrochemical Techniques D008024:Ligands D015394:Molecular Structure D009942:Organometallic Compounds D010084:Oxidation-Reduction D017382:Reactive Oxygen Species D013057:Spectrum Analysis D013329:Structure-Activity Relationship
DNA DNA cleavage Oxidation Linear dichroism Cu complex