On-line solid-phase separation/preconcentration for the determination of copper in urine by flame atomic absorption spectrometry.
A new on-line separation/preconcentration system was developed for the determination of Cu(II) ions by flame atomic absorption spectrometry in urine samples. A newly synthesized chelating resin, by anchoring eriochrome blue black R reagent to Amberlite XAD-16 resin, was used as a packing material for the selective separation/preconcentration of Cu(II) ions. The influence of the parameters on the determination of Cu(II) ions such as pH of sample solution, amount of the resin, eluent type, interfering ions and flow variables was studied. The detection limit of the method was 1.0 μg L(-1) while precision was 2.3% (n=15) at 50 μg L(-1) Cu(II) level. The adsorption capacity of the resin was 217 μg g(-1) Cu(II). The accuracy of the method was proven using TMDA-64 standard lake water and synthetic urine sample. The developed method has been applied successfully to the determination of copper in urine with satisfactory results.
['Saçmacı|Serife|S|', 'Sahan|Serkan|S|', 'Sahin|Uğur|U|', 'Kartal|Senol|S|', 'Ulgen|Ahmet|A|']
D000327:Adsorption D001391:Azo Compounds D002614:Chelating Agents D003300:Copper D005618:Fresh Water D006801:Humans D006863:Hydrogen-Ion Concentration D011108:Polymers D052616:Solid Phase Extraction D013054:Spectrophotometry, Atomic D014867:Water
Chelating resin Eriochrome blue black R On-line preconcentration Urinary copper