Bioorthogonal hydroxyethyl cellulose-based scaffold crosslinked via click chemistry for cartilage tissue engineering applications.
In this study, azide and alkyne moieties were introduced to the structure of citric acid-modified hydroxyethyl cellulose (HEC) and then through a bioorthogonal click chemistry method: Strain-promoted azide-alkyne cycloaddition, a novel crosslinked HEC scaffold (click sample) was obtained. Chemical modifications and successful crosslinking of the samples were assessed with FTIR and 1H NMR spectroscopy. Lyophilized samples exhibited a porous interconnected microarchitecture with desirable features for commensurate cartilage tissue engineering applications. As the stability of scaffolds improved upon crosslinking, considerable water uptake and swelling degree of ~650% could still be measured for the click sample. Offering Young's modulus of ~10 MPa and tensile strength of ~0.43 MPa, the mechanical characteristics of click sample were comparable with those of normal cartilage tissue. Various in vitro biological assays, including MTT analysis, cellular attachment, histological staining with safranin O, and real-time PCR decisively approved significant biocompatibility, chondrogenic ability, and bioorthogonal features of click sample.
['Nouri-Felekori|Mohammad|M|', 'Nezafati|Nader|N|', 'Moraveji|Marzie|M|', 'Hesaraki|Saeed|S|', 'Ramezani|Tayebe|T|']
D001672:Biocompatible Materials D002356:Cartilage D002448:Cell Adhesion D002460:Cell Line D002470:Cell Survival D002482:Cellulose D019902:Chondrocytes D020219:Chondrogenesis D019343:Citric Acid D057930:Click Chemistry D003432:Cross-Linking Reagents D055119:Elastic Modulus D006801:Humans D016062:Porosity D013718:Tensile Strength D023822:Tissue Engineering D054457:Tissue Scaffolds
Scaffold Crosslink Click chemistry Cartilage tissue engineering Hydroxyethyl cellulose