Prevalence of metallo-β-lactamase acquired genes among carbapenems susceptible and resistant Gram-negative clinical isolates using multiplex PCR, Khartoum hospitals, Khartoum Sudan.
BACKGROUND
The increased prevalence of carbapenem-resistant Gram-negative isolates caused by Metallo-β-lactamase (MBL) is worrisome in clinical settings worldwide. The mortality rate associated with infections caused by MBLs producing organisms ranging from 18 to 67%. This study aimed to determine the prevalence of Metallo-β-lactamase genes among some Gram-negative clinical isolates (Carbapenems susceptible and resistant).


METHODS
This paper describes a descriptive cross-sectional study carried out to detect MBL genes such as (blaVIM, blaIMP and blaNDM) by multiplex PCR mixture reaction among 200 Gram-negative clinical isolates (Citrobacter spp, Escherichia coli, Enterobacter spp, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Proteus valgaris). Khartoum hospitals during 2015 to 2016.


LIMITATION
The study organisms were not evaluated for non-MBL carbapenemases, such as KPC and OXA-48.


RESULTS
The prevalence of MBL genes by multiplex PCR assays among 200 Gram-negative clinical isolates was 72(36.1%). MBL positive genes among 100 carbapenems sensitive and 100 resistant isolates were 27(27%) and 45(45%) respectively. There was a statistically, significant association between the antimicrobial susceptibility and the presences of MBL genes (P.value = 0.008). E.coli was the predominant species possessing MBL genes 26(36.1%), with 22(30.7%) species having a combination of MBL genes. Verona integron Metallo beta-lactamase (VIM) was the most frequent genes 28(38.9%) out of 72 MBL detected genes, followed by imipenemase (IMP) was 19(26.4%), and consequently, New Delhi Metallo beta lactamase was 3(4.2%).


CONCLUSION
This study revealed a high prevalence of MBL genes in some Gram-negative isolates from Khartoum State Hospitals which were not previously established in these hospitals.
['Adam|Mudathir Abdallah|MA|', 'Elhag|Wafa I|WI|']
D000900:Anti-Bacterial Agents D015373:Bacterial Typing Techniques D015780:Carbapenems D003430:Cross-Sectional Studies D006090:Gram-Negative Bacteria D016905:Gram-Negative Bacterial Infections D006801:Humans D007711:Klebsiella pneumoniae D008826:Microbial Sensitivity Tests D060885:Multiplex Polymerase Chain Reaction D015995:Prevalence D011550:Pseudomonas aeruginosa D013397:Sudan D001618:beta-Lactamases
Carbapenem PCR Khartoum VIM IMP Gram-negative bacteria Metallo β-lactamase NDM