First evidence for thermal tolerance benefits of the bacterial symbiont Cardinium in an invasive whitefly, Bemisia tabaci.
BACKGROUD
Cardinium symbiont is a maternally inherited bacterial endosymbiont and widely spreads in arthropods including Bemisia tabaci (Hemiptera: Aleyrodidae). However, the potential role of Cardinium played in the biology of their hosts is largely unknown. In two genetic lines (i.e. LS and SG lines) of B. tabaci MED, collected from different locations in China, we tested the effects of Cardinium on the performance of the host whitefly under a constant high temperature (31 °C) using the age-stage two-sex life table method, and explored the genes influenced by Cardinium-infection by RNA-sequencing.


RESULTS
We found that Cardinium did provide protection of B. tabaci against heat stress under 31 °C. However, there was a significant connection between Cardinium-infection and whitefly genetic backgrounds. Performance revealed that Cardinium infection can increase the longevity of both female and male adults and oviposition periods in both lines, but it also conferred benefits of fecundity and pre-adult period to LS line. Additionally, the population parameters such as intrinsic rate of increase (r), finite rate of increase (λ) and mean generation time (T) demonstrated that Cardinium infection conferred fitness benefits to LS line but not to SG line. Transcriptome analysis indicated that several genes related to homeostasis and metamorphosis such as ubiquitin-related genes were highly expressed in Cardinium-infected B. tabaci.


CONCLUSION
The research provided the first evidence that Cardinium can increase the thermal tolerance of whitefly, which may be associated with host genetic background.
['Yang|Kun|K|', 'Yuan|Meng-Ying|MY|', 'Liu|Ying|Y|', 'Guo|Chen-Liang|CL|', 'Liu|Tong-Xian|TX|', 'Zhang|You-Jun|YJ|https://orcid.org/0000-0003-3508-6695', 'Chu|Dong|D|']
D000818:Animals D001419:Bacteria D005260:Female D005298:Fertility D006430:Hemiptera D008136:Longevity D008297:Male D013559:Symbiosis
thermal tolerance Cardinium Bemisia tabaci life table