Serological biomarkers for monitoring response to treatment of pulmonary and extrapulmonary tuberculosis in children and adolescents.
Key measures to halt the spread of tuberculosis (TB) include early diagnosis, effective treatment, and monitoring disease management. We sought to evaluate the use of serum immunoglobulin levels against antigens present in cell envelope of Mycobacterium tuberculosis to monitor TB treatment response in children and adolescents with pulmonary (PTB) or extrapulmonary TB (EPTB). Blood samples were collected prior to and one, two, and six months following treatment initiation. Serum immunoglobulin levels against cardiolipin, sulfatide, mycolic acid and Mce1A protein were measured by ELISA. Serum from 53 TB patients and 12 healthy participants were analyzed. After six months of successful treatment, there was a significant decrease (p < 0.0001) in IgM levels against cardiolipin, sulfatide, mycolic acid and Mce1A protein and IgG levels against Mce1A protein when compared to baseline immunoglobulin levels. There was no significant variation in antibody levels during follow-up between participants with PTB and EPTB, confirmed and unconfirmed TB diagnosis, and HIV infection status. Antibody levels in control participants without TB did not decrease during follow-up. These results suggest that immunoglobulin responses to mycobacterial cell wall products may be a useful tool to monitor treatment response in children and adolescents with PTB or EPTB.
['Dos Santos|Danielle C M|DCM|', 'Lovero|Kathryn L|KL|', 'Schmidt|Christiane M|CM|', 'Barros|Ana Cláudia M W|ACMW|', 'Quintanilha|Ana Paula|AP|', 'Barbosa|Ana Paula|AP|', 'Pone|Marcos V S|MVS|', 'Pone|Sheila M|SM|', 'Araujo|Julienne Martins|JM|', 'de Paula Martins|Camila|C|', 'Cosme|Estela Magalhães|EM|', 'Dourado de Oliveira|Thais Raquelly|TR|', 'Miceli|Ana Lúcia|AL|', 'Vieira|Maria Luíza|ML|', 'Queiroz|Adriano|A|', 'Coca Velarde|Luis Guillermo|LG|', 'Kritski|Afranio|A|', 'de Fátima Pombo March|Maria|M|', 'Maria de Azevedo Sias|Selma|S|', 'SantÀAnna|Clemax C|CC|', 'Riley|Lee W|LW|', 'Araújo Cardoso|Claudete A|CA|']
D000293:Adolescent D000367:Age Factors D000995:Antitubercular Agents D001426:Bacterial Proteins D015415:Biomarkers D002308:Cardiolipins D016022:Case-Control Studies D002648:Child D002675:Child, Preschool D016903:Drug Monitoring D004797:Enzyme-Linked Immunosorbent Assay D005260:Female D006801:Humans D007074:Immunoglobulin G D007075:Immunoglobulin M D007223:Infant D008297:Male D009169:Mycobacterium tuberculosis D009171:Mycolic Acids D011237:Predictive Value of Tests D011446:Prospective Studies D013433:Sulfoglycosphingolipids D013997:Time Factors D016896:Treatment Outcome D014397:Tuberculosis, Pulmonary
Biomarkers Monitoring Treatment Tuberculosis Pediatric