Prognostic Relevance of HJURP Expression in Patients with Surgically Resected Colorectal Cancer.
HJURP is a key factor for CENP-A deposition and maintenance in centromeres. The role of mis-regulation of histone chaperones in cancer initiation and progression has been studied. However, its role in colorectal cancer is still unclear. In this study, we aimed to evaluate the expression of HJURP in 162 colorectal cancer tissue. To investigate the function of HJURP in the colorectal cancer cell, we suppressed HJURP expression by siRNA and confirmed proliferation, migration, invasion, and anchorage independent of colony forming ability. The association between HJURP expression levels and clinicopathological factors was evaluated in 162 CRC tissues using immunohistochemistry. The overall survival rate in patients of HJURP high expression was higher than those in HJURP low expression in CRC. Suppressing HJURP expression decreased cellular proliferation, invasion, and migration in four CRC cell lines: HT29, HCT116, SW480, SW620 in vitro study. Our findings revealed that the knockdown of HJURP suppressed the proliferation, migration, invasion, and tumorigenicity in CRC cells. Due to its strong association with CRC, HJURP could be a potential prognostic biomarker and a novel target for drug discovery.
['Kang|Dong Hyun|DH|', 'Woo|Jongsoo|J|', 'Kim|Hyeongjoo|H|0000-0002-6317-1726', 'Kim|Soo Youn|SY|', 'Ji|Sanghee|S|', 'Jaygal|Gunn|G|', 'Ahn|Tae Sung|TS|', 'Kim|Han Jo|HJ|', 'Kwak|Hyoung Jong|HJ|', 'Kim|Chang-Jin|CJ|', 'Baek|Moo-Jun|MJ|', 'Jeong|Dongjun|D|0000-0001-8180-5668']
D014408:Biomarkers, Tumor D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D015179:Colorectal Neoplasms D004268:DNA-Binding Proteins D005260:Female D015972:Gene Expression Regulation, Neoplastic D045325:HCT116 Cells D019073:HT29 Cells D006801:Humans D008297:Male D011379:Prognosis D016019:Survival Analysis D016896:Treatment Outcome D015854:Up-Regulation
HJURP prognostic biomarker Colorectal cancer (CRC)