IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication.
As a disease characterized by severe liver necrosis and hemorrhage, duck viral hepatitis (DVH) is mainly caused by duck hepatitis A virus (DHAV). The positive-strand RNA genome of DHAV type 1 (DHAV-1) contains an internal ribosome entry site (IRES) element within the 5' untranslated region (UTR), structured sequence elements within the 3' UTR, and a poly(A) tail at the 3' terminus. In this study, we first examined that insulin-like growth factor-2 mRNA-binding protein-1 (IGF2BP1) specifically interacted with the DHAV-1 3' UTR by RNA pull-down assay. The interaction between IGF2BP1 and DHAV-1 3' UTR strongly enhanced IRES-mediated translation efficiency but failed to regulate DHAV-1 replication in a duck embryo epithelial (DEE) cell line. The viral propagation of DHAV-1 strongly enhanced IGF2BP1 expression level, and viral protein accumulation was identified as the key point to this increment. Collectively, our data demonstrated the positive role of IGF2BP1 in DHAV-1 viral proteins translation and provided data support for the replication mechanism of DHAV-1.
['Chen|Junhao|J|', 'Zhang|Ruihua|R|', 'Lan|Jingjing|J|', 'Lin|Shaoli|S|0000-0003-0835-5554', 'Li|Pengfei|P|', 'Gao|Jiming|J|', 'Wang|Yu|Y|', 'Xie|Zhi-Jing|ZJ|', 'Li|Fu-Chang|FC|', 'Jiang|Shi-Jin|SJ|0000-0002-4747-8410']
D020413:3' Untranslated Regions D000818:Animals D002478:Cells, Cultured D004372:Ducks D057809:HEK293 Cells D004371:Hepatitis Virus, Duck D006801:Humans D016601:RNA-Binding Proteins D014779:Virus Replication
translation efficiency duck hepatitis A virus type 1 insulin-like growth factor-2 mRNA-binding protein-1 viral propagation 3′ untranslated region