Kupffer Cell and Monocyte-Derived Macrophage Identification by Immunofluorescence on Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse Liver Sections.
Kupffer cells are the liver-resident macrophages and represent the first line of defense between the pathogens circulating from the intestines through the portal vein and systemic circulation. Recent works have highlighted the complex heterogeneity of macrophage functions and origins, thus raising awareness on the need for a better characterization of macrophage populations. The immunohistochemistry method here described, allows for a rapid distinction between Kupffer cells and monocyte-derived macrophages present on formalin-fixed, paraffin-embedded mouse liver samples. This protocol has been optimized for its reproducibility, reliability, and simplicity.
['Guillot|Adrien|A|', 'Buch|Chloé|C|', 'Jourdan|Tony|T|']
D000818:Animals D005455:Fluorescent Antibody Technique D005557:Formaldehyde D007150:Immunohistochemistry D007728:Kupffer Cells D008099:Liver D008264:Macrophages D051379:Mice D016612:Paraffin Embedding D015203:Reproducibility of Results D016707:Tissue Fixation
Kupffer cell Macrophage Immunofluorescence Liver CLEC4F/CLECSF13 Confocal microscopy FFPE liver sections IBA1 Monocyte