Hi-C 2.0: An optimized Hi-C procedure for high-resolution genome-wide mapping of chromosome conformation.
Chromosome conformation capture-based methods such as Hi-C have become mainstream techniques for the study of the 3D organization of genomes. These methods convert chromatin interactions reflecting topological chromatin structures into digital information (counts of pair-wise interactions). Here, we describe an updated protocol for Hi-C (Hi-C 2.0) that integrates recent improvements into a single protocol for efficient and high-resolution capture of chromatin interactions. This protocol combines chromatin digestion and frequently cutting enzymes to obtain kilobase (kb) resolution. It also includes steps to reduce random ligation and the generation of uninformative molecules, such as unligated ends, to improve the amount of valid intra-chromosomal read pairs. This protocol allows for obtaining information on conformational structures such as compartment and topologically associating domains, as well as high-resolution conformational features such as DNA loops.
['Belaghzal|Houda|H|', 'Dekker|Job|J|', 'Gibcus|Johan H|JH|']
D001483:Base Sequence D001710:Biotin D002460:Cell Line D002874:Chromosome Mapping D002875:Chromosomes D003432:Cross-Linking Reagents D004247:DNA D015252:Deoxyribonucleases, Type II Site-Specific D005557:Formaldehyde D015894:Genome, Human D059014:High-Throughput Nucleotide Sequencing D006801:Humans D013194:Staining and Labeling
Hi-C Chromosome conformation capture Paired-end sequencing