Cluster-determinant 36 (CD36) impacts on vitamin E postprandial response.
SCOPE
A single nucleotide polymorphism in the cluster determinant 36 (CD36) gene has recently been associated with plasma α-tocopherol concentration, suggesting a possible role of this protein in vitamin E intestinal absorption or tissue uptake.


METHODS AND RESULTS
To investigate the involvement of CD36 in vitamin E transport, we first evaluated the effect of CD36 on α- and γ-tocopherol transmembrane uptake and efflux using transfected HEK cells. γ-Tocopherol postprandial response was then assessed in CD36-deficient mice compared with wild-type mice, after the mice had been fully characterized for their α-tocopherol, vitamin A and lipid plasma, and tissue contents. Both α- and γ-tocopherol uptake was significantly increased in cells overexpressing CD36 compared with control cells. Compared with wild-type mice, CD36-deficient mice displayed a significantly decreased cholesterol hepatic concentration, and males exhibited significantly higher triacylglycerol contents in liver, brain, heart, and muscle. Although tissue α-tocopherol concentration after adjustment for lipid content was not modified, γ-tocopherol postprandial response was significantly increased in CD36-deficient mice compared with controls, likely reflecting the postprandial hypertriglyceridemia observed in these mice.


CONCLUSION
Our findings show for the first time that CD36 participates-directly or indirectly-in vitamin E uptake, and that CD36 effect on postprandial lipid metabolism in turn modifies vitamin E postprandial response.
['Goncalves|Aurélie|A|', 'Roi|Stéphanie|S|', 'Nowicki|Marion|M|', 'Niot|Isabelle|I|', 'Reboul|Emmanuelle|E|']
D000818:Animals D018955:CD36 Antigens D002784:Cholesterol D005260:Female D057809:HEK293 Cells D006801:Humans D007408:Intestinal Absorption D050356:Lipid Metabolism D008099:Liver D008297:Male D051379:Mice D020641:Polymorphism, Single Nucleotide D019518:Postprandial Period D014280:Triglycerides D014801:Vitamin A D024502:alpha-Tocopherol D024504:gamma-Tocopherol
CD36 α-Tocopherol Intestinal absorption γ-Tocopherol Bioavailability Transgenic mice