Essential Oil from Bark of Aniba parviflora (Meisn.) Mez (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model.
Aniba parviflora (Meisn.) Mez (Lauraceae) is an aromatic plant of the Amazon rainforest, which has a tremendous commercial value in the perfumery industry; it is popularly used as flavoring sachets and aromatic baths. In Brazilian folk medicine, A. parviflora is used to treat victims of snakebites. Herein, we analyzed the chemical composition of A. parviflora bark essential oil (EO) and its effect on the growth of human hepatocellular carcinoma HepG2 cells in vitro and in vivo. EO was obtained by hydrodistillation and characterized by GC-MS and GC-FID. The main constituents of EO were linalool (16.3±3.15), α-humulene (14.5±2.41 %), δ-cadinene (10.2±1.09 %), α-copaene (9.51±1.12 %) and germacrene B (7.58±2.15 %). Initially, EO's cytotoxic effect was evaluated against five cancer cell lines (HepG2, MCF-7, HCT116, HL-60 and B16-F10) and one non-cancerous one (MRC-5), using the Alamar blue method after 72 h of treatment. The calculated IC50 values were 9.05, 22.04, >50, 15.36, 17.57, and 30.46 μg/mL, respectively. The best selectivity was for HepG2 cells with a selective index of 3.4. DNA Fragmentation and cell cycle distribution were quantified in HepG2 cells by flow cytometry after a treatment period of 24 and 48 h. The effect of EO on tumor development in vivo was evaluated in a xenograft model using C.B-17 SCID mice engrafted with HepG2 cells. In vivo tumor growth inhibition of HepG2 xenograft at the doses of 40 and 80 mg/kg were 12.1 and 62.4 %, respectively.
['de Oliveira|Felipe P|FP|http://orcid.org/0000-0002-7120-5084', 'da C Rodrigues|Ana Carolina B|ACB|', 'de Lima|Emilly J S P|EJSP|http://orcid.org/0000-0001-9866-6342', 'Silva|Valdenizia R|VR|http://orcid.org/0000-0001-8717-0501', 'de S Santos|Luciano|L|http://orcid.org/0000-0002-1381-1342', 'da Anunciação|Talita A|TA|http://orcid.org/0000-0002-8376-9741', 'Nogueira|Mateus L|ML|http://orcid.org/0000-0003-3308-6587', 'Soares|Milena B P|MBP|http://orcid.org/0000-0001-7549-2992', 'Dias|Rosane B|RB|http://orcid.org/0000-0002-9491-334X', 'Gurgel Rocha|Clarissa A|CA|http://orcid.org/0000-0001-8922-2985', 'Duvoisin Junior|Sérgio|S|http://orcid.org/0000-0003-2577-7898', 'Albuquerque|Patrícia M|PM|http://orcid.org/0000-0001-8614-7676', 'Lima|Emerson S|ES|http://orcid.org/0000-0002-9367-2812', 'Gonçalves|José F C|JFC|http://orcid.org/0000-0001-9197-4617', 'Bataglion|Giovana A|GA|http://orcid.org/0000-0001-6643-9268', 'Costa|Emmanoel V|EV|http://orcid.org/0000-0002-0153-822X', 'da Silva|Felipe M A|FMA|http://orcid.org/0000-0002-1809-1372', 'Koolen|Hector H F|HHF|http://orcid.org/0000-0002-0181-348X', 'Bezerra|Daniel P|DP|http://orcid.org/0000-0002-6774-2063']
D000818:Animals D000972:Antineoplastic Agents, Phytogenic D002460:Cell Line D049109:Cell Proliferation D002470:Cell Survival D004305:Dose-Response Relationship, Drug D004354:Drug Screening Assays, Antitumor D056945:Hep G2 Cells D006801:Humans D019770:Lauraceae D008114:Liver Neoplasms, Experimental D051379:Mice D016513:Mice, SCID D009822:Oils, Volatile D024301:Plant Bark D013237:Stereoisomerism D013329:Structure-Activity Relationship
Aniba parviflora Lauraceae HepG2 cytotoxicity antitumor agents