Interaction of Alpha-synuclein with Cytogaligin, a protein encoded by the proapoptotic gene GALIG.
GALIG, an internal gene to the human galectin-3 gene, encodes two distinct proteins, Mitogaligin and Cytogaligin through translation of a unique mRNA in two overlapping alternative reading frames. When overexpressed GALIG induces apoptosis. In cultured cells, Mitogaligin destabilizes mitochondria membranes through interaction with cardiolipin. Little is known regarding the role of Cytogaligin. This protein displays multiple subcellular localizations; cytosol, nucleus, and mitochondria. We illustrate here that Cytogaligin is also secreted in the extracellular medium. Cytogaligin is shown to interact with α-Synuclein, the major component of Lewy bodies in Parkinson's disease. Overexpression of Cytogaligin reduces α-Synuclein dimerization raising a possible role in the evolution of α-Synuclein aggregation, a key molecular event underlying the pathogenesis of Parkinson's disease.
['El Haddad|Saïd|S|', 'Serrano|Amandine|A|', 'Normand|Thierry|T|', 'Robin|Chloé|C|', 'Dubois|Martine|M|', 'Brulé-Morabito|Fabienne|F|', 'Mollet|Lucile|L|', 'Charpentier|Stéphane|S|', 'Legrand|Alain|A|']
D017209:Apoptosis D051017:Apoptosis Regulatory Proteins D001798:Blood Proteins D045604:Extracellular Fluid D037161:Galectins D006367:HeLa Cells D006801:Humans D011485:Protein Binding D025941:Protein Interaction Mapping D013347:Subcellular Fractions D051844:alpha-Synuclein
Alpha-synuclein Cytogaligin GALIG gene Luciferase protein-fragment complementation assay