Chemical gelling of hydrogels-based biological macromolecules for tissue engineering: Photo- and enzymatic-crosslinking methods.
Newly, injectable hydrogels have been renowned as promising biomaterials and appropriate candidates for tissue engineering which can be applied for the development of 3-dimensional cell culture models. Hydrogels have in situ formability that allows an actual and homogeneous drugs/cells encapsulation, and suitable for in vivo surgical operation in a minimally invasive way, causing less discomfort for patients. A wide and varied range of methods has been applied to design hydrogels-based biological macromolecules via chemical gelling techniques, such as photo-polymerization, and enzyme-catalyzed reactions due to the biocompatibility and feasible processing of in situ formation of hydrogels and the easy implantation through in situ injection of hydrogels-based biological macromolecules. This present review covers the current advances in the development of injectable hydrogels through enzymatically and photo-crosslinking procedures for tissue engineering. The characteristics and applications of natural and synthetic base materials used in hydrogel generation are also reviewed with an outline on biomedical considerations.
['Nezhad-Mokhtari|Parinaz|P|', 'Ghorbani|Marjan|M|', 'Roshangar|Leila|L|', 'Soleimani Rad|Jafar|J|']
D000818:Animals D001672:Biocompatible Materials D002384:Catalysis D018929:Cell Culture Techniques D002470:Cell Survival D019902:Chondrocytes D003094:Collagen D003432:Cross-Linking Reagents D004338:Drug Combinations D004798:Enzymes D006801:Humans D020100:Hydrogels D007267:Injections D007797:Laminin D051379:Mice D041681:NIH 3T3 Cells D010006:Osteoblasts D010777:Photochemistry D011108:Polymers D011509:Proteoglycans D023822:Tissue Engineering
Tissue engineering Hydrogels Enzymatically-crosslinking Photochemically cross-linking