HLA-B influences integrin beta-1 expression and pancreatic cancer cell migration.
Human leukocyte antigen (HLA) class I molecules present antigenic peptides to cytotoxic T cells, causing lysis of malignant cells. Transplantation biology studies have implicated HLA class I molecules in cell migration, but there has been little evidence presented that they influence cancer cell migration, a contributing factor in metastasis. In this study, we examined the effect of HLA-B on pancreatic cancer cell migration. HLA-B siRNA transfection increased the migration of the S2-013 pancreatic cancer cells but, in contrast, reduced migration of the PANC-1 and MIA PaCa-2 pancreatic cancer cell lines. Integrin molecules have previously been implicated in the upregulation of pancreatic cancer cell migration, and knockdown of HLA-B in S2-013 cells heightened the expression of integrin beta 1 (ITGB1), but in the PANC-1 and MIA PaCa-2 cells HLA-B knockdown diminished ITGB1 expression. A transmembrane sequence in an S2-013 HLA-B heavy chain matches a corresponding sequence in HLA-B in the BxPC-3 pancreatic cancer cell line, and knockdown of BxPC-3 HLA-B mimics the effect of S2-013 HLA-B knockdown on migration. In total, our findings indicate that HLA-B influences the expression of ITGB1 in pancreatic cancer cells, with concurrent distinctions in transmembrane sequences and effects on the migration of the cells.
['Sliker|Bailee H|BH|', 'Goetz|Benjamin T|BT|', 'Barnes|Raina|R|', 'King|Hannah|H|', 'Maurer|H Carlo|HC|', 'Olive|Kenneth P|KP|', 'Solheim|Joyce C|JC|']
D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D051417:Focal Adhesion Kinase 1 D015972:Gene Expression Regulation, Neoplastic D015235:HLA-B Antigens D006801:Humans D039421:Integrin alpha2 D019012:Integrin beta1 D009928:Organ Specificity D010179:Pancreas D020033:Protein Isoforms D034741:RNA, Small Interfering D015398:Signal Transduction
Integrin Pancreatic cancer Migration HLA Major histocompatibility complex class I