10,12 Conjugated Linoleic Acid-Driven Weight Loss Is Protective against Atherosclerosis in Mice and Is Associated with Alternative Macrophage Enrichment in Perivascular Adipose Tissue.
The dietary fatty acid 10,12 conjugated linoleic acid (10,12 CLA) promotes weight loss by increasing fat oxidation, but its effects on atherosclerosis are less clear. We recently showed that weight loss induced by 10,12 CLA in an atherosclerosis-susceptible mouse model with characteristics similar to human metabolic syndrome is accompanied by accumulation of alternatively activated macrophages within subcutaneous adipose tissue. The objective of this study was to evaluate whether 10,12 CLA-mediated weight loss was associated with an atheroprotective phenotype. Male low-density lipoprotein receptor deficient (Ldlr-/-) mice were made obese with 12 weeks of a high-fat, high-sucrose diet feeding (HFHS: 36% fat, 36% sucrose, 0.15% added cholesterol), then either continued on the HFHS diet with or without caloric restriction (CR), or switched to a diet with 1% of the lard replaced by either 9,11 CLA or 10,12 CLA for 8 weeks. Atherosclerosis and lipid levels were quantified at sacrifice. Weight loss in mice following 10,12 CLA supplementation or CR as a weight-matched control group had improved cholesterol and triglyceride levels, yet only the 10,12 CLA-treated mice had improved en face and aortic sinus atherosclerosis. 10,12 CLA-supplemented mice had increased lesion macrophage content, with enrichment of surrounding perivascular adipose tissue (PVAT) alternative macrophages, which may contribute to the anti-atherosclerotic effect of 10,12 CLA.
['Kanter|Jenny E|JE|', 'Goodspeed|Leela|L|', 'Wang|Shari|S|', 'Kramer|Farah|F|', 'Wietecha|Tomasz|T|', 'Gomes-Kjerulf|Diego|D|', 'Subramanian|Savitha|S|', "O'Brien|Kevin D|KD|", 'den Hartigh|Laura J|LJ|']
D000273:Adipose Tissue D000818:Animals D050197:Atherosclerosis D031204:Caloric Restriction D059305:Diet, High-Fat D019422:Dietary Sucrose D019587:Dietary Supplements D044243:Linoleic Acids, Conjugated D008262:Macrophage Activation D008264:Macrophages D008297:Male D051379:Mice D018345:Mice, Knockout D009765:Obesity D011973:Receptors, LDL D015431:Weight Loss
perivascular adipose tissue alternatively activated macrophages type 2 cytokines