Calreticulin is highly expressed in pancreatic cancer stem-like cells.
Cancer stem-like cells (CSLCs) in solid tumors are thought to be resistant to conventional chemotherapy or molecular targeting therapy and to contribute to cancer recurrence and metastasis. In this study, we aimed to identify a biomarker of pancreatic CSLCs (P-CSLCs). A P-CSLC-enriched population was generated from pancreatic cancer cell lines using our previously reported method and its protein expression profile was compared with that of parental cells by 2-D electrophoresis and tandem mass spectrometry. The results indicated that a chaperone protein calreticulin (CRT) was significantly upregulated in P-CSLCs compared to parental cells. Flow cytometry analysis indicated that CRT was mostly localized to the surface of P-CSLCs and did not correlate with the levels of CD44v9, another P-CSLC biomarker. Furthermore, the side population in the CRThigh /CD44v9low population was much higher than that in the CRTlow /CD44v9high population. Calreticulin expression was also assessed by immunohistochemistry in pancreatic cancer tissues (n = 80) obtained after radical resection and was found to be associated with patients' clinicopathological features and disease outcomes in the Cox proportional hazard regression model. Multivariate analysis identified CRT as an independent prognostic factor for pancreatic cancer patients, along with age and postoperative therapy. Our results suggest that CRT can serve as a biomarker of P-CSLCs and a prognostic factor associated with poorer survival of pancreatic cancer patients. This novel biomarker can be considered as a therapeutic target for cancer immunotherapy.
['Matsukuma|Satoshi|S|', 'Yoshimura|Kiyoshi|K|', 'Ueno|Tomio|T|', 'Oga|Atsunori|A|', 'Inoue|Moeko|M|', 'Watanabe|Yusaku|Y|', 'Kuramasu|Atsuo|A|', 'Fuse|Masanori|M|', 'Tsunedomi|Ryouichi|R|', 'Nagaoka|Satoshi|S|', 'Eguchi|Hidetoshi|H|', 'Matsui|Hiroto|H|', 'Shindo|Yoshitaro|Y|', 'Maeda|Noriko|N|', 'Tokuhisa|Yoshihiro|Y|', 'Kawano|Reo|R|', 'Furuya-Kondo|Tomoko|T|', 'Itoh|Hiroshi|H|', 'Yoshino|Shigefumi|S|', 'Hazama|Shoichi|S|', 'Oka|Masaaki|M|', 'Nagano|Hiroaki|H|']
D018528:ATP-Binding Cassette Transporters D051928:CD47 Antigen D037282:Calreticulin D045744:Cell Line, Tumor D006801:Humans D018960:Hyaluronan Receptors D053208:Kaplan-Meier Estimate D014411:Neoplastic Stem Cells D010190:Pancreatic Neoplasms D011379:Prognosis D016016:Proportional Hazards Models D040901:Proteomics
calreticulin pancreatic cancer Biomarkers cancer stem cells proteomics