In Vitro Evaluation of Osteoblast Response to the Effect of Injectable Platelet-rich Fibrin Coating on Titanium Disks.
AIM
Biomimetic implant surface coatings can improve osteoblast response and enhance osseointegration. This study aimed to assess the response of osteoblast-like cell line (MG-63) coating of injectable platelet-rich fibrin on titanium discs.


MATERIALS AND METHODS
Injectable-PRF (i-PRF) was prepared by centrifugation of blood at 700 rpm for 3 minutes without any anti-coagulant in i-PRF tubes. Ten commercially pure titanium discs were divided into control groups comprising plain discs, and test group, titanium discs were coated with i-PRF. These were then added to the cultured MG-63 cells. Cell proliferation, alkaline phosphatase production, and mineralization were assessed in both groups at day 1, 7, 14, and 21.


RESULTS
The cell proliferation, alkaline phosphatase production, and mineralization increased significantly from day 1 to day 21 in both test and control groups and was significantly higher in the test group than in control group at day 1, 7, 14, and 21 (p <0.001).


CONCLUSIONS
Coating of titanium discs with i-PRF causes increased proliferation, alkaline phosphatase production, and increased mineralization at day 1, 7, 14, and 21 in MG-63 osteoblast-like cells.


CLINICAL SIGNIFICANCE
Improved osteoblast proliferation and mineralization demonstrate enhanced activity on the surface of an implant, which in turn may lead to increased bone to implant contact and faster/ and/or enhanced osseointegration.
['Shah|Rucha|R|', 'Thomas|Raison|R|', 'Gowda|Triveni Mavinkote|TM|', 'Baron|Tarun Kumar Amanna|TKA|', 'Vemanaradhya|Gayathri Gunjiganur|GG|', 'Bhagat|Shivani|S|']
D002478:Cells, Cultured D016348:Osseointegration D010006:Osteoblasts D000073183:Platelet-Rich Fibrin D013499:Surface Properties D014025:Titanium
Osseointegration Bone Dental implants Osteoblasts Platelet-rich fibrin.