Production of biomass and lipids by the oleaginous microalgae Monoraphidium sp. QLY-1 through heterotrophic cultivation and photo-chemical modulator induction.
A two-step strategy comprising heterotrophic cultivation and photo-chemical modulator induction was developed to enhance biomass and lipid accumulation in the oleaginous Monoraphidium sp. QLY-1, which was isolated from Qilu Lake in Yunnan Plateau. The algae were first cultivated heterotrophically to achieve high biomass concentration (5.54gL(-1)) with a lipid content of 22.47%. The cultivated algae were diluted, transferred to light environment, and treated with different chemical elicitors. Results showed that the lipid content increased to 36.68% after 3-day of photoinduction. The lipid content was further enhanced by 1.21, 1.32, and 1.29 folds in algal cells treated with nitrogen deficiency, 20gL(-1) NaCl, and 5mM glycine betaine, respectively. The maximum lipid content (48.54%) and lipid productivity (121.27mgL(-1)d(-1)) were obtained in treatments with 20gL(-1) NaCl and 5mM GB, respectively. This study proposes a strategy to efficiently produce lipids by using microalgae.
['Zhao|Yongteng|Y|', 'Li|Dafei|D|', 'Ding|Ke|K|', 'Che|Raoqiong|R|', 'Xu|Jun-Wei|JW|', 'Zhao|Peng|P|', 'Li|Tao|T|', 'Ma|Huixian|H|', 'Yu|Xuya|X|']
D001622:Betaine D018533:Biomass D000460:Chlorophyta D005227:Fatty Acids D052836:Heterotrophic Processes D008055:Lipids D058086:Microalgae D055668:Photochemical Processes D052817:Phototrophic Processes D010802:Phylogeny D012337:RNA, Ribosomal, 18S D017382:Reactive Oxygen Species D012965:Sodium Chloride
Monoraphidium sp. QLY-1 NaCl Glycine betaine Lipid induction Two-step cultivation