Differential embryotoxicity of the organic pollutants in rural and urban air particles.
Airborne particulate matter (PM) is a recognized risk factor for human populations. Here we assessed the toxic potential of the organic constituents from PM collected in urban and rural sites during warm and cold periods of 2012/2013, and fractionated into 6 size fractions. The finest PM fraction (<0.5 μm) showed the highest biological activity (dioxin-like activity and fish embryotoxicity) in all samples, and the maximal activity was observed in rural samples from the cold period. Zebrafish embryo transcriptome analysis showed a strong induction of the AhR signaling pathway correlated to PAH concentrations. Oxidative stress-related genes and pancreatic and eye-lens gene markers appeared de-regulated in embryos exposed to urban extracts, whereas exposure to rural extracts affected genes implicated in basic cellular functions. The observed effects can be directly related to air pollution-related human disorders, suggesting different potential adverse outcomes for human populations exposed to air pollution from specific sources.
['Mesquita|Sofia R|SR|', 'van Drooge|Barend L|BL|', 'Oliveira|Eva|E|', 'Grimalt|Joan O|JO|', 'Barata|Carlos|C|', 'Vieira|Natividade|N|', 'Guimarães|Laura|L|', 'Piña|Benjamin|B|']
D000393:Air Pollutants D000818:Animals D004625:Embryo, Nonmammalian D047108:Embryonic Development D004784:Environmental Monitoring D018507:Gene Expression Regulation, Developmental D063990:Gene Ontology D006801:Humans D007908:Lens, Crystalline D010179:Pancreas D010316:Particle Size D052638:Particulate Matter D018570:Risk Assessment D012423:Rural Health D014504:Urban Health D015027:Zebrafish
Air pollution Air quality Danio rerio Embryo toxicity GC–MS PM10 Toxicogenomics