Bone marrow mesenchymal stem cells combined with minocycline improve spinal cord injury in a rat model.
The aims of this study were to assess that the effects of bone marrow mesenchymal stem cells (BMSCs) combination with minocycline improve spinal cord injury (SCI) in rat model. In the present study, the Wistar rats were randomly divided into five groups: control group, SCI group, BMSCs group, Minocycline group and BMSCs + minocycline group. Basso, Beattie and Bresnahan (BBB) test and MPO activity were used to assess the effect of combination therapy on locomotion and neutrophil infiltration. Inflammation factors, VEGF and BDNF expression, caspase-3 activation, phosphorylation-p38MAPK, proNGF, p75NTR and RhoA expressions were estimated using commercial kits or western blot, respectively. BBB scores were significantly increased and MPO activity was significantly undermined by combination therapy. In addition, combination therapy significantly decreased inflammation factors in SCI rats. Results from western blot showed that combination therapy significantly up-regulated the protein of VEGF and BDNF expression and down-regulated the protein of phosphorylation-p38MAPK, proNGF, p75NTR and RhoA expressions in SCI rats. Combination therapy stimulation also suppressed the caspase-3 activation in SCI rats. These results demonstrated that the effects of bone marrow mesenchymal stem cells combination with minocycline improve SCI in rat model.
['Chen|Dayong|D|', 'Zeng|Wei|W|', 'Fu|Yunfeng|Y|', 'Gao|Meng|M|', 'Lv|Guohua|G|']
D000818:Animals D000893:Anti-Inflammatory Agents D017209:Apoptosis D015153:Blotting, Western D016026:Bone Marrow Transplantation D004195:Disease Models, Animal D005260:Female D008297:Male D045164:Mesenchymal Stem Cell Transplantation D008911:Minocycline D051381:Rats D017207:Rats, Sprague-Dawley D017208:Rats, Wistar D020127:Recovery of Function D013119:Spinal Cord Injuries
Bone marrow mesenchymal stem cells minocycline spinal cord injury inflammation VEGF and BDNF