Enzymatic degradation of Elephant grass (Pennisetum purpureum) stems: influence of the pith and bark in the total hydrolysis.
The internal pith of a high energy plant, Elephant grass (EG), was more extensively degraded (>50% dry matter) compared to the outer cortex (31%) or the whole stem (35%) by an enzyme preparation from Humicola insolens, Ultraflo. Reducing sugars and acetic acid release from the pith was also higher compared to the cortex. Supplementation of Ultraflo with a type-C feruloyl esterase increased the level of deacetylation but also led to reduced solubilisation. The addition of 20% dimethyl sulfoxide (DMSO) as a co-solvent also reduced the solubility of EG by Ultraflo, although acetic acid release was increased, complimenting previous results found on model substrates. The presence of DMSO was also shown to have a protective effect on xylanase activity but not acetyl esterase activity in Ultraflo. Xylan in the biomass was preferentially solubilised by DMSO, while Ultraflo removed more glucose than xylose.
['Pérez-Boada|Marta|M|', 'Prieto|Alicia|A|', 'Prinsen|Pepijn|P|', 'Forquin-Gomez|Marie-Pierre|MP|', 'del Río|José Carlos|JC|', 'Gutiérrez|Ana|A|', 'Martínez|Ángel T|ÁT|', 'Faulds|Craig B|CB|']
D019342:Acetic Acid D018533:Biomass D002241:Carbohydrates D002265:Carboxylic Ester Hydrolases D004121:Dimethyl Sulfoxide D004798:Enzymes D006868:Hydrolysis D008031:Lignin D031782:Pennisetum D024301:Plant Bark D018547:Plant Stems D012995:Solubility D013997:Time Factors
Co-solvents Acetyl esterase Biomass utilisation Energy crop Glycoside hydrolases