Depression-like behavior associated with E/I imbalance of mPFC and amygdala without TRPC channels in mice of knockout IL-10 from microglia.
Depression has a growing impact on public health. Accumulating evidence supports an association between depression and increased immune system activity. IL-10 is a key cytokine that inhibits excessive inflammatory responses and is related to the anti-inflammatory and protective functions of the central nervous system (CNS). Cx3cr1CreERIL-10-/- mice were used in our study. We aimed to identify the role of IL-10 in microglia in depression and anxiety-like behavior. We performed a series of behavioral tests on the mice; the Cx3cr1CreERIL-10-/- male mice showed depression- and anxiety-like behavior compared with the littermates. The expression of transient receptor potential canonical 5 (TRPC5) decreased in both the medial prefrontal cortex (mPFC) and amygdala regions. The cytokines IL-1β and IL-6 increased, and IL-10 was decreased by western blotting. The knockout mice showed different trends in the effects of synaptic proteins. In the mPFC, IL-10 knockout induced a decrease in NR2B and synaptophysin; in the amygdala region, there was a significant increase in NR2B and PSD95. IL-10 knockout from microglia induced a decrease in GAD67 and parvalbumin (Pv) in the mPFC, but not in the amygdala. Our results showed enhanced depression and anxiety-like behavior in the Cx3cr1CreER IL-10-/- mice, which could be related to an imbalance in local excitatory and inhibitory transmission, as well as neuroinflammation in the mPFC and amygdala. This imbalance was associated with increased local inflammation. Although many studies have demonstrated the role of TRPC channels in emotional responses, our study showed that TRPC was not involved in this process in Cx3cr1CreERIL-10-/- mice.
['Yang|Liang|L|', 'Liu|Chang|C|', 'Li|Weiya|W|', 'Ma|Yunqing|Y|', 'Huo|Shiji|S|', 'Ozathaley|Ahsawle|A|', 'Ren|Jiling|J|', 'Yuan|Wenjian|W|', 'Ni|Hong|H|', 'Li|Dong|D|', 'Zhang|Jing|J|', 'Liu|Zhaowei|Z|']
D000679:Amygdala D000818:Animals D003863:Depression D016753:Interleukin-10 D008297:Male D051379:Mice D008810:Mice, Inbred C57BL D018345:Mice, Knockout D017628:Microglia D017397:Prefrontal Cortex D050052:TRPC Cation Channels
E/I imbalance TRPC IL-10 Microglia Depression