Quantitative determination of saroglitazar, a predominantly PPAR alpha agonist, in human plasma by a LC-MS/MS method utilizing electrospray ionization in a positive mode.
A sensitive LC-MS/MS method was developed and validated for quantitation of saroglitazar using turboion spray interface with positive ion mode. A liquid-liquid extraction, with a mixture of dichloromethane and diethyl ether, was employed for the extraction of saroglitazar and glimepiride (IS) from human plasma. The chromatographic separation was achieved using an ACE-5, C18 (4.6 × 100 mm) column with a gradient mobile phase comprising acetonitrile and ammonium acetate buffer with trifluoracetic acid in purified water. Both analytes were separated within 10 min with retention times of 4.52 and 2.57 min for saroglitazar and IS, respectively. Saroglitazar quantitation was achieved by the summation of two MRM transition pairs (m/z 440.2 to m/z 366.0 and m/z 440.2 to m/z 183.1), while that of IS was achieved using transition pair m/z 491.3 to m/z 352.0. The calibration standards of saroglitazar showed linearity from 0.2 to 500 ng/mL, with a lower limit of quantitation of 0.2 ng/mL. The biases for inter- and intra-batch assays were -7.51-1.15% and -11.21 to -3.25%, respectively, while the corresponding precisions were 5.04-8.06% and 1.53-7.68%, respectively. The developed method was used to monitor the plasma concentrations of saroglitazar in clinical samples.
['Ghoghari|Ashok|A|', 'Dash|Ranjeet|R|', 'Bhatt|Chandrakant|C|', 'Singh|Kanchan|K|', 'Jha|Anil|A|', 'Patel|Harilal|H|', 'Gupta|Rahul|R|', 'Kansagra|Kevinkumar|K|', 'Srinivas|Nuggehally R|NR|']
D002138:Calibration D002853:Chromatography, Liquid D006801:Humans D057230:Limit of Detection D047493:PPAR alpha D010666:Phenylpropionates D011758:Pyrroles D012015:Reference Standards D015203:Reproducibility of Results D021241:Spectrometry, Mass, Electrospray Ionization D053719:Tandem Mass Spectrometry
saroglitazar plasma LC-MS/MS Lipaglyn™ PPAR-α method validation pharmacokinetics