Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation.
The isolation and culture of intestinal stem cells (ISCs) was first demonstrated in the very recent decade with the identification of ISC marker Lgr5. The growth of ISCs into crypt organoids provides an in vitro model for studying the mucosal physiology, intestinal cancer tumorigenesis, and intestinal regeneration. Here, we describe two different isolation protocols and demonstrate a fixation method that aids in the confocal observation of the organoids.
['Chen|Yun|Y|', 'Li|Chuan|C|', 'Tsai|Ya-Hui|YH|', 'Tseng|Sheng-Hong|SH|']
D018929:Cell Culture Techniques D002469:Cell Separation D002478:Cells, Cultured D006801:Humans D066298:In Vitro Techniques D007422:Intestines D018613:Microscopy, Confocal D009940:Organoids D013234:Stem Cells
Crypt organoid Isolation Intestinal stem cell (ISC) Confocal observation Three-dimensional imaging