Independent and combined effects of environmental factors and miR-126, miR-143, and miR-145 on the risk of coronary heart disease.
Objective
To evaluate the effects of environmental factors and microRNAs (miRNAs) (miR-126, miR-143, and miR-145) on the risk of coronary heart disease (CHD).


Methods
A frequency-matched case-control study (450 patients, 450 controls) was conducted from April 2014 to December 2016 in Fuzhou City, China. Environmental factors were investigated using a self-administered questionnaire, and the expression levels of miR-126, miR-143, and miR-145 were determined by quantitative real-time Polymerase Chain Reaction (PCR) in peripheral blood mononuclear cells. Unconditional logistic regression models were used for statistical evaluation.


Results
Alcohol consumption, high-salt diets, high-intensity work, and lack of physical activity were significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. MiR-126, miR-143, and miR-145 were highly expressed in the CHD group compared with the control group. After adjustment for other environmental factors, unconditional logistic regression results revealed that miR-126, miR-143, and depression were the independent risk factors of CHD, and light diet was the independent protective factor of CHD.


Conclusions
Our data suggest that a family history of CHD, anxiety, and alcohol consumption was significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. Furthermore, miR-126 and miR-143 in combination with several risk factors, could play a joint role in the development of CHD. Therefore, it is necessary to manage patients with CHD in all directions and multiple level.
['Lin|Da-Cen|DC|', 'Lin|Jia-Bing|JB|', 'Chen|Zhou|Z|', 'Chen|Rong|R|', 'Wan|Chun-Yu|CY|', 'Lin|Shao-Wei|SW|', 'Ruan|Qi-Shuang|QS|', 'Li|Huang-Yuan|HY|', 'Wu|Si-Ying|SY|']
Environmental factors MiR-126 MiR-143 MiR-145 Coronary heart disease Case-control study Gene-environment interaction