RNA polymerase II promoter-proximal pausing in mammalian long non-coding genes.
Mammalian genomes encode a large number of non-coding RNAs (ncRNAs) that greatly exceed mRNA genes. While the physiological and pathological roles of ncRNAs have been increasingly understood, the mechanisms of regulation of ncRNA expression are less clear. Here, our genomic study has shown that a significant number of long non-coding RNAs (lncRNAs, >1000 nucleotides) harbor RNA polymerase II (Pol II) engaged with the transcriptional start site. A pausing and transcriptional elongation factor for protein-coding genes, tripartite motif-containing 28 (TRIM28) regulates the transcription of a subset of lncRNAs in mammalian cells. In addition, the majority of lncRNAs in human and murine cells regulated by Pol II promoter-proximal pausing appear to function in stimulus-inducible biological pathways. Our findings suggest an important role of Pol II pausing for the transcription of mammalian lncRNA genes.
['Bunch|Heeyoun|H|', 'Lawney|Brian P|BP|', 'Burkholder|Adam|A|', 'Ma|Duanduan|D|', 'Zheng|Xiaofeng|X|', 'Motola|Shmulik|S|', 'Fargo|David C|DC|', 'Levine|Stuart S|SS|', 'Wang|Yaoyu E|YE|', 'Hu|Guang|G|']
D000818:Animals D002478:Cells, Cultured D005786:Gene Expression Regulation D023281:Genomics D057809:HEK293 Cells D006801:Humans D008322:Mammals D051379:Mice D000066450:Mouse Embryonic Stem Cells D009687:Nuclear Proteins D011401:Promoter Regions, Genetic D012319:RNA Polymerase II D062085:RNA, Long Noncoding D012097:Repressor Proteins D014158:Transcription, Genetic D000076183:Tripartite Motif-Containing Protein 28
RNA polymerase II promoter-proximal pausing Long non-coding RNAs TRIM28