Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile.
Advanced engineering of materials for the development of drug delivery devices provides scope for novel and versatile strategies for treatment of various diseases. 'Electrospraying' was used to prepare PLGA microparticles and further encapsulate the drug, metronidazole (Met) within the particles to function as a drug delivery system. Two different solvents were utilized for the preparation of drug loaded PLGA particles, whereby the polymeric solution in dichloromethane (DCM) produced particles of bigger sizes than using trifluoroethanol (TFE). Scanning electron microscopy showed the spherical morphology of the particles, with sizes of 3946±407nm and 1774±167nm, respectively for PLGA-Met(DCM) and PLGA-Met(TFE). The FTIR spectroscopy proved the incorporation of metronidazole in the polymer, but without any specific drug-polymer interaction. The release of the drug from the particles was studied in phosphate buffered saline, where a sustained drug release was obtained for at least 41days. Cytotoxicity evaluation of the drug extract using mesenchymal stem cells (MSCs) showed not hindering the proliferation of MSCs, and the cell phenotype was retained after incubation in the drug containing media. Electrospraying is suggested as a cost-effective and single step process for the preparation of polymeric microparticles for prolonged and controlled release of drug.
['Prabhakaran|Molamma P|MP|', 'Zamani|Maedeh|M|', 'Felice|Betiana|B|', 'Ramakrishna|Seeram|S|']
D049109:Cell Proliferation D002478:Cells, Cultured D003692:Delayed-Action Preparations D004337:Drug Carriers D016503:Drug Delivery Systems D006801:Humans D019344:Lactic Acid D059630:Mesenchymal Stem Cells D008752:Methylene Chloride D008795:Metronidazole D008855:Microscopy, Electron, Scanning D010316:Particle Size D011100:Polyglycolic Acid D000077182:Polylactic Acid-Polyglycolic Acid Copolymer D011108:Polymers D012997:Solvents D013499:Surface Properties D014270:Trifluoroethanol
Electrospraying Release Drug delivery Stem cells Particle size