Development-Dependent Changes in the NR2 Subtype of the N-Methyl-D-Aspartate Receptor in the Suprachiasmatic Nucleus of the Rat.
The suprachiasmatic nucleus (SCN) is the main brain clock that regulates circadian rhythms in mammals. The SCN synchronizes to the LD cycle through the retinohypothalamic tract (RHT), which projects to ventral SCN neurons via glutamatergic synapses. Released glutamate activates N-methyl-D-aspartate (NMDA) receptors, which play a critical role in the activation of signaling cascades to enable phase shifts. Previous evidence indicates that presynaptic changes during postnatal development consist of an increase in RHT fibers impinging on SCN neurons between postnatal day (P) 1 to 4 and P15. The aim of this study was to evaluate postsynaptic developmental changes in the NR2 subunits that determine the pharmacological and biophysical properties of the neuronal NMDA receptors in the ventral SCN. To identify the expression of NR2 subtypes, we utilized RT-PCR, immunohistochemical fluorescence, and electrophysiological recordings of synaptic activity. We identified development-dependent changes in NR2A, C, and D subtypes in mRNA and protein expression, whereas NR2B protein was equally present at all analyzed postnatal ages. The NR2A antagonist PEAQX (100 nM) reduced the frequency of NMDA excitatory postsynaptic currents (EPSCs) at P8 significantly more than at P34, but the antagonists for NR2B (3 μM Ro 25-6981) and NR2C/D (150 nM PPDA) did not influence NMDA EPSCs differently at the 2 analyzed postnatal ages. Our results point to P8 as the earliest analyzed postnatal age that shows mRNA and protein expression similar to those found at the juvenile stage P34. Taken together, our findings indicate that postsynaptic development-dependent modifications in the NR2 subtypes of the NMDA receptor could be important for the synchronization of ventral SCN neurons to the LD cycle at adult stages.
['Herrera-Zamora|J Manuel|JM|', 'Castro-Sánchez|Luis A|LA|', 'Reyes-Mendez|Miriam|M|', 'Aguilar-Martinez|Irving|I|', 'Osuna-López|Fernando|F|', 'Moreno-Galindo|Eloy G|EG|', 'Navarro-Polanco|Ricardo A|RA|', 'Aguilar-Roblero|Raul A|RA|0000-0001-6213-9313', 'Sánchez-Pastor|Enrique|E|', 'Alamilla|Javier|J|0000-0002-9666-5490']
D000375:Aging D000818:Animals D001921:Brain D002940:Circadian Rhythm D051381:Rats D017208:Rats, Wistar D016194:Receptors, N-Methyl-D-Aspartate D000074523:Suprachiasmatic Nucleus Neurons D013569:Synapses
NR2 SCN retinohypothalamic tract glutamate NMDA development