Modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers.
Background
Prevention of bacterial colonization remains a major challenge in the field of oral implant devices. Chimeric peptides with binding, antimicrobial, and osteogenesis motifs may provide a promising alternative for the inhibition of biofilm formation on titanium (Ti) surfaces.


Methods
In this study, chimeric peptides were designed by connecting an antimicrobial sequence from human β-defensin-3 with a Ti-binding sequence and arginine-glycine-aspartic acid using a glycine-glycine-glycine linker. Binding to the Ti substrate and antimicrobial properties against streptococci were evaluated. Significant improvement in reduction of bacterial colonization onto the Ti surface was observed, with or without the presence of saliva or serum. The MC3T3-E1 cells grew well on the modified Ti surfaces compared with the control group.


Results
The data showed that the three peptide functional motifs maintained their respective functions, and that the antibiofilm mechanism of the chimeric peptide was via suppression of sspA and sspB gene expression.


Conclusion
These results indicated that the endogenous peptide fragments engineered on the Ti surface could provide an environmentally friendly approach for improving the biocompatibility of oral implants.
['Zhang|Xi|X|', 'Geng|Hongjuan|H|', 'Gong|Lei|L|', 'Zhang|Qian|Q|', 'Li|Hongjie|H|', 'Zhang|Xu|X|', 'Wang|Yonglan|Y|', 'Gao|Ping|P|']
D000327:Adsorption D000595:Amino Acid Sequence D000818:Animals D000890:Anti-Infective Agents D017209:Apoptosis D001419:Bacteria D018441:Biofilms D002460:Cell Line D002942:Circular Dichroism D015169:Colony Count, Microbial D006801:Humans D051379:Mice D008826:Microbial Sensitivity Tests D009842:Oligopeptides D013499:Surface Properties D014025:Titanium D023083:beta-Defensins
multifunctional chimeric peptide human β-defensin-3 Ti-binding peptide-1 arginine-glycine-aspartic acid-containing peptides modification of titanium surface