Production of β-fructofuranosidase with transfructosylating activity by Aspergillus tamarii URM4634 Solid-State Fermentation on agroindustrial by-products.
Eight different Aspergillus strains were tested for their ability to produce β-fructofuranosidase (FFase) by Solid-State Fermentation. The Aspergillus tamarii URM4634 strain was selected as the most performant and tested on six different agroindustrial by-products. Soy, wheat and oat brans, which allowed for the highest hydrolytic (UH) and transfructosylating (UTF) activities, were tested individually or in mixtures according to a simplex-centroid mixture design in order to investigate their effects on FFase production at different times. The best results in terms of both enzyme activities were obtained with only soy bran. The influence of substrate, moisture and sucrose levels on FFase production was evaluated, and the highest UH and UTF activities were 229.43 ± 4.88 and 66.93 ± 3.02 U/mL, respectively. The obtained results indicate that A. tamarii FFase may be a biocatalyst with great potential for industrial applications such as sugar inversion and fructo-oligosaccharides production.
['Oliveira|Rodrigo Lira de|RL|', 'Silva|Marcos Fellipe da|MFD|', 'Converti|Attilio|A|', 'Porto|Tatiana Souza|TS|']
D001230:Aspergillus D005285:Fermentation D005632:Fructose D005947:Glucose D006868:Hydrolysis D009844:Oligosaccharides D013379:Substrate Specificity D043324:beta-Fructofuranosidase
β-Fructofuranosidase Fermentation Agroindustrial by-products Mixture design Fructo-oligosaccharides