Characterization of novel microsatellite markers for Hyphantria cunea and implications for other Lepidoptera.
This is the first report of microsatellite markers (simple sequence repeats, SSR) for fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae), an important quarantine pest in some European and Asian countries. Here, we developed 48 microsatellite markers for H. cunea from SSR enrichment libraries. Sequences isolated from libraries were sorted into four categories and analyzed. Our results suggest that sequences classified as Grouped should not be used for microsatellite primer design. The genetic diversity of microsatellite loci was assessed in 72 individuals from three populations. The number of alleles per locus ranged from 2 to 5 with an average of 3. The observed and expected heterozygosities of loci ranged from 0 to 0.958 and 0 to 0.773, respectively. A total of 18 out of 153 locus/population combinations deviated significantly from Hardy-Weinberg equilibrium. Moreover, significant linkage disequilibrium was detected in one pair of loci (1275 pairs in total). In the neutral test, two loci were grouped into the candidate category for positive selection and the remainder into the neutral category. In addition, a complex mutation pattern was observed for these loci, and F ST performed better than did R ST for the estimation of population differentiation in different mutation patterns. The results of the present study can be used for population genetic studies of H. cunea.
['Cao|L J|LJ|', 'Wen|J B|JB|', 'Wei|S J|SJ|', 'Liu|J|J|', 'Yang|F|F|', 'Chen|M|M|']
D000818:Animals D015723:Gene Library D006580:Genetic Carrier Screening D014644:Genetic Variation D005828:Genetics, Population D015810:Linkage Disequilibrium D018895:Microsatellite Repeats D009036:Moths D009154:Mutation D013045:Species Specificity
microsatellite Hyphantria cunea Lepidoptera mutation pattern population differentiation