Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier Systems.
A native strain of the heterocytous cyanobacterium Trichormus variabilis VRUC 168 was mass cultivated in a low-cost photobioreactor for a combined production of Polyunsaturated Fatty Acids (PUFA) and Exopolymeric Substances (EPS) from the same cyanobacterial biomass. A sequential extraction protocol was optimized leading to high yields of Released EPS (REPS) and PUFA, useful for nutraceutical products and biomaterials. REPS were extracted and characterized by chemical staining, Reversed Phase-High-Performance Liquid Chromatography (RP-HPLC), Fourier Transform Infrared Spectroscopy (FT-IR) and other spectroscopic techniques. Due to their gelation property, REPS were used to produce a photo-polymerizable hybrid hydrogel (REPS-Hy) with addition of polyethylene glycol diacrylated (PEGDa). REPS-Hy was stable over time and resistant to dehydration and spontaneous hydrolysis. The rheological and functional properties of REPS-Hy were studied. The enzyme carrier ability of REPS-Hy was assessed using the detoxification enzyme thiosulfate:cyanide sulfur transferase (TST), suggesting the possibility to use REPS-Hy as an enzymatic hydrogel system. Finally, REPS-Hy was used as a scaffold for culturing human mesenchymal stem cells (hMSCs). The cell seeding onto the REPS-Hy and the cell embedding into 3D-REPS-Hy demonstrated a scaffolding property of REPS-Hy with non-cytotoxic effect, suggesting potential applications of cyanobacteria REPS for producing enzyme- and cell-carrier systems.
['Bellini|Erika|E|', 'Ciocci|Matteo|M|', 'Savio|Saverio|S|', 'Antonaroli|Simonetta|S|', 'Seliktar|Dror|D|', 'Melino|Sonia|S|', 'Congestri|Roberta|R|']
D001672:Biocompatible Materials D018533:Biomass D018929:Cell Culture Techniques D002460:Cell Line D000458:Cyanobacteria D019587:Dietary Supplements D004337:Drug Carriers D005231:Fatty Acids, Unsaturated D006801:Humans D020100:Hydrogels D059630:Mesenchymal Stem Cells D058433:Photobioreactors D011092:Polyethylene Glycols D017550:Spectroscopy, Fourier Transform Infrared D054457:Tissue Scaffolds
Trichormus variabilis Cyanobacteria PUFA biomaterials hydrogel enzyme mesenchymal stem cells Extracellular Polymeric Substances omega 3