Study on the Plasma Protein Binding Rate and Compatibility Regularity of the Constituents Migrating to Blood of Simiao Yong'an Decoction.
OBJECTIVE
To study the compatibility regularity of Simiao Yong'an decoction by determining the plasma protein binding rate with the constituents in Simiao Yong'an decoction and to preliminarily clarify the effects of the compatibility on the plasma protein binding rate of different components.


METHODS
Based on the equilibrium dialysis method, high-performance liquid chromatography was used to determine the contents of six constituents, which were divided into a single group and combination groups, in Simiao Yong'an decoction in the internal and external dialysis solutions. The obtained plasma protein binding rate through calculations was an index to evaluate the binding of the above components to plasma protein in different conditions.


RESULTS
Harpagide, harpagoside, sweroside and loganin showed low plasma protein binding rates, ferulic acid exhibited a moderate plasma protein binding rate, and glycyrrhizic acid showed a high plasma protein binding rate. The compatibility study showed that glycyrrhizic acid promoted the binding of ferulic acid to plasma protein. Glycyrrhizic acid and ferulic acid were the key compounds to promote the binding of harpagide to plasma protein. Glycyrrhizic acid, harpagide, harpagoside and loganin had a significant inhibitory effects on the binding of sweroside to plasma protein. The plasma protein binding capacities of harpagoside and loganin were reduced by the other five constituents. Glycyrrhizic acid had the strongest plasma protein binding effect, and the binding effect was not affected by other components.


CONCLUSION
This study explores the effects of compound compatibility on effective components from the perspective of plasma protein binding by high-performance liquid chromatography combined with the equilibrium dialysis method, and lays a foundation for clarifying the compatibility rule of Simiao Yong'an decoction and also provides a new idea for the study of the compatibility of traditional Chinese medicine formulas.
['Yu|Xiao|X|', 'Jiao|Qishu|Q|', 'Jiang|Yanyan|Y|', 'Guo|Shuzhen|S|', 'Zhang|Wei|W|', 'Liu|Bin|B|']
D002851:Chromatography, High Pressure Liquid D003373:Coumaric Acids D006027:Glycosides D019695:Glycyrrhizic Acid D039823:Iridoids D064209:Phytochemicals D011485:Protein Binding D011714:Pyrans D012710:Serum Albumin, Bovine
Plasma protein binding rate equilibrium dialysis Simiao Yong’an decoction drug interaction pharmacokinetics traditional Chinese medicine compound