Genetic profiling of protein burden and nuclear export overload.
Overproduction (op) of proteins triggers cellular defects. One of the consequences of overproduction is the protein burden/cost, which is produced by an overloading of the protein synthesis process. However, the physiology of cells under a protein burden is not well characterized. We performed genetic profiling of protein burden by systematic analysis of genetic interactions between GFP-op, surveying both deletion and temperature-sensitive mutants in budding yeast. We also performed genetic profiling in cells with overproduction of triple-GFP (tGFP), and the nuclear export signal-containing tGFP (NES-tGFP). The mutants specifically interacted with GFP-op were suggestive of unexpected connections between actin-related processes like polarization and the protein burden, which was supported by morphological analysis. The tGFP-op interactions suggested that this protein probe overloads the proteasome, whereas those that interacted with NES-tGFP involved genes encoding components of the nuclear export process, providing a resource for further analysis of the protein burden and nuclear export overload.
['Kintaka|Reiko|R|', 'Makanae|Koji|K|0000-0002-8560-4561', 'Namba|Shotaro|S|', 'Kato|Hisaaki|H|', 'Kito|Keiji|K|0000-0002-9057-1688', 'Ohnuki|Shinsuke|S|', 'Ohya|Yoshikazu|Y|0000-0003-0837-1239', 'Andrews|Brenda J|BJ|', 'Boone|Charles|C|', 'Moriya|Hisao|H|0000-0001-7638-3640']
D021581:Active Transport, Cell Nucleus D002467:Cell Nucleus D000076610:Genetic Profile D023281:Genomics D049452:Green Fluorescent Proteins D009154:Mutation D049790:Nuclear Export Signals D046988:Proteasome Endopeptidase Complex D014176:Protein Biosynthesis D012441:Saccharomyces cerevisiae D029701:Saccharomyces cerevisiae Proteins
genetic profiling genetics protein burden nuclear export S. cerevisiae genomics overexpression