Flow field-flow fractionation and multi-angle light scattering as a powerful tool for the characterization and stability evaluation of drug-loaded metal-organic framework nanoparticles.
Asymmetric flow field-flow fractionation (AF4) coupled with UV-Vis spectroscopy, multi-angle light scattering (MALS) and refractive index (RI) detection has been applied for the characterization of MIL-100(Fe) nanoMOFs (metal-organic frameworks) loaded with nucleoside reverse transcriptase inhibitor (NRTI) drugs for the first time. Empty nanoMOFs and nanoMOFs loaded with azidothymidine derivatives with three different degrees of phosphorylation were examined: azidothymidine (AZT, native drug), azidothymidine monophosphate (AZT-MP), and azidothymidine triphosphate (AZT-TP). The particle size distribution and the stability of the nanoparticles when interacting with drugs have been determined in a time frame of 24 h. Main achievements include detection of aggregate formation in an early stage and monitoring nanoMOF morphological changes as indicators of their interaction with guest molecules. AF4-MALS proved to be a useful methodology to analyze nanoparticles engineered for drug delivery applications and gave fundamental data on their size distribution and stability. Graphical abstract ᅟ.
['Roda|Barbara|B|', 'Marassi|Valentina|V|', 'Zattoni|Andrea|A|', 'Borghi|Francesco|F|', 'Anand|Resmi|R|', 'Agostoni|Valentina|V|', 'Gref|Ruxandra|R|', 'Reschiglian|Pierluigi|P|', 'Monti|Sandra|S|']
D019380:Anti-HIV Agents D000963:Antimetabolites D056831:Coordination Complexes D054306:Dideoxynucleotides D004337:Drug Carriers D000067493:Dynamic Light Scattering D031521:Fractionation, Field Flow D000073396:Metal-Organic Frameworks D008958:Models, Molecular D053758:Nanoparticles D010316:Particle Size D012031:Refractometry D013056:Spectrophotometry, Ultraviolet D013942:Thymine Nucleotides D015215:Zidovudine
Flow field-flow fractionation Multi-angle light scattering NRTI Azidothymidine Metal–organic framework nanoparticles characterization Metal–organic frameworks