Increased numbers of CD23(+) CD21(hi) Bin-like B cells in human reactive and rheumatoid arthritis lymph nodes.
A unique population of CD23(+) CD21(high) B cells in inflamed nodes (Bin) has been shown to accumulate in lymph nodes (LNs) draining inflamed joints of TNF-transgenic (TNF-tg) mice. Bin cells contribute to arthritis flare in mice by distorting node architecture and hampering lymphatic flow, but their existence in human inflamed LNs has not yet been described. Here, we report the characterization of resident B-cell populations in fresh popliteal lymph nodes (PLNs) from patients with severe lower limb diseases (non-RA) and rheumatoid arthritis (RA) patients, and from banked, cryopreserved reactive and normal human LN single cell suspension samples. Bin-like B cells were shown to be significantly increased in reactive LNs, and strikingly elevated (>30% of total) in RA samples. Histopathology and immunofluorescence analyses were consistent with B follicular hyperplasia and histological alterations in RA vs. non-RA PLNs. This is the first description of Bin-like B cells in human inflamed LNs. Consistent with published mouse data, this population appears to be associated with inflammatory arthritis and distortion of LN architecture. Further analyses are necessary to assess the role of CD23(+) CD21(hi) Bin-like B cells in RA pathogenesis and arthritic flare.
['Kuzin|Igor I|II|', 'Kates|Stephen L|SL|', 'Ju|Yawen|Y|', 'Zhang|Longze|L|', 'Rahimi|Homaira|H|', 'Wojciechowski|Wojciech|W|', 'Bernstein|Steven H|SH|', 'Burack|Richard|R|', 'Schwarz|Edward M|EM|', 'Bottaro|Andrea|A|']
D000818:Animals D001172:Arthritis, Rheumatoid D016175:B-Lymphocyte Subsets D015415:Biomarkers D006801:Humans D016130:Immunophenotyping D008198:Lymph Nodes D018655:Lymphocyte Count D051379:Mice D008822:Mice, Transgenic D017464:Receptors, Complement 3d D017455:Receptors, IgE
B cells Rheumatoid arthritis Lymph nodes Inflammation TNF