Pacsin 2 is required for the maintenance of a normal cardiac function in the developing mouse heart.
The Pacsin proteins (Pacsin 1, 2 and 3) play an important role in intracellular trafficking and thereby signal transduction in many cells types. This study was designed to examine the role of Pacsin 2 in cardiac development and function. We investigated the development and electrophysiological properties of Pacsin 2 knockout (P2KO) hearts and single cardiomyocytes isolated from 11.5 and 15.5days old fetal mice. Immunofluorescence experiments confirmed the lack of Pacsin 2 protein expression in P2KO cardiac myocytes in comparison to wildtype (WT). Western blotting demonstrates low expression levels of connexin 43 and T-box 3 proteins in P2KO compared to wildtype (WT). Electrophysiology measurements including online Multi-Electrode Array (MEA) based field potential (FP) recordings on isolated whole heart of P2KO mice showed a prolonged AV-conduction time. Patch clamp measurements of P2KO cardiomyocytes revealed differences in action potential (AP) parameters and decreased pacemaker funny channel (If), as well as L-type Ca2+ channel (ICaL), and sodium channel (INa). These findings demonstrate that Pacsin 2 is necessary for cardiac development and function in mouse embryos, which will enhance our knowledge to better understand the genesis of cardiovascular diseases.
['Semmler|Judith|J|', 'Kormann|Jan|J|', 'Srinivasan|Sureshkumar Perumal|SP|', 'Köster|Annette|A|', 'Sälzer|Daniel|D|', 'Reppel|Michael|M|', 'Hescheler|Jürgen|J|', 'Plomann|Markus|M|', 'Nguemo|Filomain|F|']
D000200:Action Potentials D048868:Adaptor Proteins, Signal Transducing D000818:Animals D003598:Cytoskeletal Proteins D047108:Embryonic Development D005260:Female D006321:Heart D008297:Male D008810:Mice, Inbred C57BL D018345:Mice, Knockout D011506:Proteins
Pacsin 2 AV conduction Developing heart Ion channels Knockout mouse Myocardial function