Regenerative effect of mesenteric fat stem cells on ccl4-induced liver cirrhosis, an experimental study.
Background
Liver cirrhosis is a chronic disease in which normal liver tissue is replaced by fibrous tissue, leads to liver malfunction. Although transplantation is the most certain cure, stem cell therapies are shedding light on efforts to regenerate cirrhotic liver. The purpose of this study was to evaluate the regenerative potential of mesenteric fat stem cells in CCL4-induced liver cirrhosis in an animal model.


Methods
Thirty rats were treated with the mixture of CCL4 and olive oil intraperitoneally by a dose of 0.2 ml (0.1 ml CCL4 and 0.1 ml olive oil) every other day for 16 weeks till cirrhosis signs appeared. Fifteen rats were randomly selected as control group. Others treated by mesenteric fat derived mesenchymal stem cells transferred into the liver parenchyma.


Results
After 5 weeks, rats received stem cells had improved clinically by increased movements, appetite, improvement in overall behavior and decreased abdomen size. Histopathologically, liver cells showed state of regeneration and forming new colonies.


Conclusion
Liver cirrhosis was induced. The mesenchymal stem cells derived from mesenteric adipose tissue could improve hepatic status of the rats, as cirrhotic livers were regenerated back into normal appearing parenchyma. Rats' clinical behavior also reached healthy status.
['Nazhvani|Fatemeh Dehghani|FD|', 'Haghani|Iman|I|', 'Nazhvani|Seifollah Dehghani|SD|', 'Namazi|Fatemeh|F|', 'Ghaderi|Abbas|A|']
Animal model ADSCs, Multipotent adipose-derived stem cells ALT, Alanine transaminase AST, Aspartate transaminase Adipose-derived mesenchymal stem cells CCL4, Carbon tetracholoride CNS, Central nervous system EDTA, Ethylenediaminetetraacetic acid FBS, Fetal bovine serum HGF, Hepatocyte growth factor Hepatic cirrhosis IM, Intramuscular IP, Intraperitoneal Liver fibrosis MSCs, Mesenchymal stem cells PBS, Phosphate buffered saline VEGF, Vascular endothelial growth factor α-MEM, Minimum essential medium α