Oxytocin improves proliferation and neural differentiation of adipose tissue-derived stem cells.
The neurohypophyseal hormone oxytocin plays a role in stimulation of neurogenesis in the adult brain. However, the exact role of oxytocin in neural development is still not well understood. In the present study, we evaluated the effect of oxytocin on neural differentiation of mouse adipose tissue-derived stem cells (ADSCs). For this purpose, ADSCs were cultured in a medium containing Knockout™ Serum Replacement (KoSR) and treated with different concentrations of oxytocin at the first or eighth day of differentiation. Two weeks after neural induction, ADSCs expressed several early and late neuron-specific genes and proteins. MTT assay and cell cycle analysis revealed a stimulatory effect of oxytocin on viability and proliferation of differentiating ADSCs. As detected by quantitative real-time PCR, treatment of the ADSCs with low concentrations of oxytocin induced neurogenesis. Oxytocin treatment also upregulated the expression of oxytocin receptor mRNA. These results demonstrated for the first time that oxytocin treatment can promote neural differentiation of the ADSCs in a dose-dependent and time-dependent manner. Oxytocin has a significant role in neurogenesis, and this may have implications in regeneration of adult neurons.
['Jafarzadeh|Nazli|N|', 'Javeri|Arash|A|', 'Khaleghi|Maryam|M|', 'Taha|Masoumeh Fakhr|MF|']
D000273:Adipose Tissue D000818:Animals D002454:Cell Differentiation D049109:Cell Proliferation D002478:Cells, Cultured D051379:Mice D058953:Neural Stem Cells D055495:Neurogenesis D010121:Oxytocin D010751:Phosphopyruvate Hydratase D018809:Proliferating Cell Nuclear Antigen D018045:Receptors, Oxytocin
Oxytocin Proliferation Differentiation Neurogenesis ADSC