Intercellular Calcium Signaling Induced by ATP Potentiates Macrophage Phagocytosis.
Extracellular ATP is a signaling molecule exploited by the immune cells for both autocrine regulation and paracrine communication. By performing live calcium imaging experiments, we show that triggered mouse macrophages are able to propagate calcium signals to resting bystander cells by releasing ATP. ATP-based intercellular communication is mediated by P2X4 and P2X7 receptors and is a feature of pro-inflammatory macrophages. In terms of functional significance, ATP signaling is required for efficient phagocytosis of pathogen-derived molecules and apoptotic cells and may represent a target for macrophage regulation by CD39-expressing cells. These results highlight a cell-to-cell communication mechanism tuning innate immunity.
['Zumerle|Sara|S|', 'Calì|Bianca|B|', 'Munari|Fabio|F|', 'Angioni|Roberta|R|', 'Di Virgilio|Francesco|F|', 'Molon|Barbara|B|', 'Viola|Antonella|A|']
D000255:Adenosine Triphosphate D000818:Animals D019898:Autocrine Communication D020013:Calcium Signaling D002450:Cell Communication D002478:Cells, Cultured D005110:Extracellular Space D005260:Female D008264:Macrophages D051379:Mice D008810:Mice, Inbred C57BL D010587:Phagocytosis D000067996:RAW 264.7 Cells
calcium macrophage phagocytosis P2X receptors adenosine triphosphate