Newcastle disease virus-vectored West Nile fever vaccine is immunogenic in mammals and poultry.
BACKGROUND
West Nile virus (WNV) is an emerging zoonotic pathogen which is harmful to human and animal health. Effective vaccination in susceptible hosts should protect against WNV infection and significantly reduce viral transmission between animals and from animals to humans. A versatile vaccine suitable for different species that can be delivered via flexible routes remains an essential unmet medical need. In this study, we developed a recombinant avirulent Newcastle disease virus (NDV) LaSota strain expressing WNV premembrane/envelope (PrM/E) proteins (designated rLa-WNV-PrM/E) and evaluated its immunogenicity in mice, horses, chickens, ducks and geese.


RESULTS
Mouse immunization experiments disclosed that rLa-WNV-PrM/E induces significant levels of WNV-neutralizing antibodies and E protein-specific CD4+ and CD8+ T-cell responses. Moreover, recombinant rLa-WNV-PrM/E elicited significant levels of WNV-specific IgG in horses upon delivery via intramuscular immunization, and in chickens, ducks and geese via intramuscular, oral or intranasal immunization.


CONCLUSIONS
Our results collectively support the utility of rLa-WNV-PrM/E as a promising WNV veterinary vaccine candidate for mammals and poultry.
['Wang|Jinliang|J|', 'Yang|Jie|J|', 'Ge|Jinying|J|', 'Hua|Ronghong|R|', 'Liu|Renqiang|R|', 'Li|Xiaofeng|X|', 'Wang|Xijun|X|', 'Shao|Yu|Y|', 'Sun|Encheng|E|', 'Wu|Donglai|D|', 'Qin|Chengfeng|C|', 'Wen|Zhiyuan|Z|', 'Bu|Zhigao|Z|']
D000818:Animals D000914:Antibodies, Viral D002645:Chickens D005260:Female D005822:Genetic Vectors D006801:Humans D008322:Mammals D051379:Mice D009522:Newcastle disease virus D011201:Poultry Diseases D014611:Vaccination D014759:Viral Envelope Proteins D014765:Viral Vaccines D014901:West Nile Fever D014902:West Nile virus
West Nile fever Mammal Poultry Neutralizing antibody Newcastle disease virus vectored vaccine T cell response