Modulation of CAT-2B-Mediated l-Arginine Uptake and Nitric Oxide Biosynthesis in HCT116 Cell Line Through Biological Activity of 4'-Geranyloxyferulic Acid Extract from Quinoa Seeds.
Chenopodium quinoa Wild is a "pseudocereal" grain which attracts a lot of attention in the scientific community as it has a positive effect on health. Here, we investigate the presence of biologically active O-prenylated phenylpropanoids in the ethanol extract of commercially available quinoa seeds. We claim that 4'-Geranyloxyferulic acid (GOFA) was the only phytochemical product found that belongs to quinoa's group secondary metabolites. We studied the changes in the oxidative and inflammatory status of the cellular environment in HCT 116 cell line processed with quinoa extract and its component GOFA; the implementation was done through the analysis of the antioxidant enzymes (SOD and CAT), the pro-inflammatory components (iNOS, IL-6 and TNF-α), and the products of intermediary metabolism (ONOO-, O2-). Moreover, the l-arginine uptake was proposed as a target of the tested compounds. We demonstrated that the GOFA, through a decrease of the CAT-2B expression, leads to a reduction of the l-arginine uptake, downregulating the harmful iNOS and restoring the altered redox state. These results propose a new molecular target involved in the reduction of the critical inflammatory process responsible for the cancer progression.
['Franceschelli|Sara|S|0000-0002-1580-6520', 'Gatta|Daniela Maria Pia|DMP|0000-0002-7513-3129', 'Pesce|Mirko|M|', 'Ferrone|Alessio|A|', 'Quiles|José Luis|JL|0000-0002-9048-9086', 'Genovese|Salvatore|S|', 'Epifano|Francesco|F|', 'Fiorito|Serena|S|', 'Taddeo|Vito Alessandro|VA|', 'Patruno|Antonia|A|', 'Grilli|Alfredo|A|', 'Felaco|Mario|M|', 'Speranza|Lorenza|L|0000-0002-7024-3257']
D016588:Anticarcinogenic Agents D001120:Arginine D027202:Cationic Amino Acid Transporter 2 D027465:Chenopodium quinoa D003373:Coumaric Acids D045325:HCT116 Cells D006801:Humans D007249:Inflammation D009369:Neoplasms D009569:Nitric Oxide D018384:Oxidative Stress D012639:Seeds
l-arginine nitric oxide IL-6 TNF-α CAT-2B inflammation oxidative stress