The Endo-β-1,4-Glucanase of Bacillus amyloliquefaciens Is Required for Optimum Endophytic Colonization of Plants.
The eglS gene in Bacillus amyloliquefaciens encodes an endo-β-1,4-glucanase that belongs to glycosyl hydrolase family 5. In this study, a disruption mutant of gene eglS was constructed to examine its role in bacterial adaptation in plants. The mutant TB2k, eglS gene inactivated bacterial strain, was remarkably impaired in extracellular cellulase activity. When inoculated on Brassica campestris, the TB2k population was reduced by more than 60% compared with the wild-type strain in the root, stem, and leaf tissues. Overexpression of eglS in the wild-type strain increased the bacteria population in the plant tissues. Further studies revealed that the transcription level of eglS was correlated with bacterial population. These data demonstrate that endo-β-1,4-glucanase of B. amyloliquefaciens is required for its optimal endophytic colonization.
['Fan|Xiaojing|X|', 'Yang|Ruixian|R|', 'Qiu|Sixin|S|', 'Cai|Xueqing|X|', 'Zou|Huasong|H|', 'Hu|Fangping|F|']
D000069977:Bacillus amyloliquefaciens D001426:Bacterial Proteins D001483:Base Sequence D001937:Brassica D002480:Cellulase D003001:Cloning, Molecular D060026:Endophytes D004789:Enzyme Activation D005798:Genes, Bacterial D016296:Mutagenesis D009154:Mutation D012336:RNA, Ribosomal, 16S D011994:Recombinant Proteins D015533:Transcriptional Activation
endo-β-1,4-glucanase Bacillus amyloliquefaciens endophytic colonization