Effective and site-specific phosphoramidation reaction for universally labeling nucleic acids.
Here we report efficient and selective postsynthesis labeling strategies, based on an advanced phosphoramidation reaction, for nucleic acids of either synthetic or enzyme-catalyzed origin. The reactions provided phosphorimidazolide intermediates of DNA or RNA which, whether reacted in one pot (one-step) or purified (two-step), were directly or indirectly phosphoramidated with label molecules. The acquired fluorophore-labeled nucleic acids, prepared from the phosphoramidation reactions, demonstrated labeling efficacy by their F/N ratio values (number of fluorophores per molecule of nucleic acid) of 0.02-1.2 which are comparable or better than conventional postsynthesis fluorescent labeling methods for DNA and RNA. Yet, PCR and UV melting studies of the one-step phosphoramidation-prepared FITC-labeled DNA indicated that the reaction might facilitate nonspecific hybridization in nucleic acids. Intrinsic hybridization specificity of nucleic acids was, however, conserved in the two-step phosphoramidation reaction. The reaction of site-specific labeling nucleic acids at the 5'-end was supported by fluorescence quenching and UV melting studies of fluorophore-labeled DNA. The two-step phosphoramidation-based, effective, and site-specific labeling method has the potential to expedite critical research including visualization, quantification, structural determination, localization, and distribution of nucleic acids in vivo and in vitro.
['Su|Yu-Chih|YC|', 'Chen|Hsing-Yin|HY|', 'Ko|Ni Chien|NC|', 'Hwang|Chi-Ching|CC|', 'Wu|Min Hui|MH|', 'Wang|Li-Fang|LF|', 'Wang|Yun-Ming|YM|', 'Chang|Sheng-Nan|SN|', 'Wang|Eng-Chi|EC|', 'Wang|Tzu-Pin|TP|']
D000577:Amides D004247:DNA D016650:Fluorescein-5-isothiocyanate D005456:Fluorescent Dyes D010766:Phosphorylation D012313:RNA
Phosphoramidation Labeling Nucleic acid Postsynthesis Site specific