Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil.
Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.
['Ribeiro|Geovani de Oliveira|GO|', 'Monteiro|Fred Julio Costa|FJC|', 'Rego|Marlisson Octavio da S|MODS|', "Ribeiro|Edcelha Soares D'Athaide|ESD|", 'Castro|Daniela Funayama de|DF|', 'Caseiro|Marcos Montani|MM|', 'Souza Marinho|Robson Dos Santos|RDS|', 'Komninakis|Shirley Vasconcelos|SV|', 'Witkin|Steven S|SS|', 'Deng|Xutao|X|', 'Delwart|Eric|E|', 'Sabino|Ester Cerdeira|EC|', 'da Costa|Antonio Charlys|AC|', 'Leal|Élcio|É|']
D000330:Aedes D000818:Animals D001938:Brazil D003465:Culex D005260:Female D059014:High-Throughput Nucleotide Sequencing D056186:Metagenomics D030102:Orthoreovirus, Mammalian D012367:RNA, Viral D012324:RNA-Dependent RNA Polymerase D065947:Rainforest D012088:Reoviridae Infections
RNA-dependent RNA polymerase Hubei reo-like virus 7 Aedes aegypti Culex quinquefasciatus mosquitoes Brazil birds Amazon forest arbovirus insect-viruses metagenomic reovirus