The effect of a bioactive tissue-engineered sling in a rat of stress incontinence model.
In this study, we attempt to examine the feasibility of the bioactive tissue-engineered sling by using muscle precursor cells (MPCs)-seeded Poly(ε-caprolactone) (PCL) nanofiber sheet in a rat model of stress urinary incontinence (SUI). In vitro, MPCs were cultured on a PCL nanofiber sheet for one week, where the MPCs-seeded PCL nanofiber sheet showed constant twitching contraction by electrical field stimulation in an organ bath. In vivo, MPCs-seeded PCL nanofiber sheet was placed under the female rat's urethra after pudendal nerve denervation (animal model of SUI). The leak point pressure (LPP) was evaluated with the vertical tilt table after the operation for four weeks. The resulting LPP of MPCs-seeded PCL nanofiber sheet group was observed to be significantly higher than the denervation-only group's. Furthermore, PKH-26-labeled MPCs were observed under the urethral sphincter by immunohistochemistry. These results indicated that, the MPCs-seeded PCL nanofiber sheet have not only provided support for the deficient sphincter, but also actively improved the sphincter's function overall. In conclusion, this bioactive tissue-engineered sling could be used as an ideal material for the treatment of SUI.
['Kim|In Gul|IG|', 'Piao|Shuyu|S|', 'Hong|Sung Hoo|SH|', 'Kim|Sae Woong|SW|', 'Hwang|Tae Kon|TK|', 'Oh|Se Heang|SH|', 'Lee|Jin Ho|JH|', 'Lee|Ji Youl|JY|']
D000818:Animals D001672:Biocompatible Materials D004195:Disease Models, Animal D005260:Female D009121:Muscle Denervation D009132:Muscles D057139:Nanofibers D011091:Polyesters D011312:Pressure D017207:Rats, Sprague-Dawley D013234:Stem Cells D053825:Suburethral Slings D023822:Tissue Engineering D014521:Urethra D014550:Urinary Incontinence, Stress
sling muscle precursor cell nanofiber stress urinary incontinence urethral sphincter