Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.
The ability to activate and regulate stem cells during wound healing and tissue/organ regeneration is a promising field which could bring innovative approaches to regenerative medicine. The regenerative capacity of invertebrates has been well documented, however in mammals, stem cells that drive organ regeneration are rare. Deer antler is unique in providing a mammalian model of complete organ regeneration based on stem cells. The present study investigated the differentially regulated proteins (DRPs) between different antler stem cell populations (n = 3) using 2D-DIGE. Western blotting was used to validate the proteomics results. Comparative proteomics resulted in protein profiles which were similar for the biological replicates but different between the cells derived from two different stem cell niches involved in antler growth/regeneration and cells derived from facial periosteum. Ninety-two up- and down-regulated proteins were identified by MALDI-TOF MS. The work indicates that the epithelial-mesenchymal transition process may participate in the initiation of antler regeneration including the first stage of scar-less wound healing. Cell mobility is also highly regulated during antler regeneration. Energy and nucleotide metabolism may however be less active in antler regeneration as compared to that in antler generation phase. These results provide new insights into the underlying mechanisms of stem cell-based regeneration of mammalian organs.
['Dong|Zhen|Z|', 'Coates|Dawn|D|', 'Liu|Qingxiu|Q|', 'Sun|Hongmei|H|', 'Li|Chunyi|C|']
D000818:Animals D000999:Antlers D003670:Deer D008954:Models, Biological D040901:Proteomics D012038:Regeneration D013234:Stem Cells
Proteomics Deer Antler stem cell Regenerative medicine 2D-DIGE Periosteum