Neurotherapeutic and antioxidant response of D-ribose-L-Cysteine nutritional dietary supplements on Alzheimer-type hippocampal neurodegeneration induced by cuprizone in adult male wistar rat model.
INTRODUCTION
Cuprizone is a neurotoxicant causing neurodegeneration through enzymes inhibition and oxidative stress. D-Ribose-L-Cysteine (DRLC) is a powerful antioxidant with neuroprotective properties. This study explored the antioxidant response of DRLC against cuprizone-induced behavioral alterations, biochemical imbalance and hippocampal neuronal damage in adult wistar rats.


MATERIALS AND METHODS
Thirty two (32) adult male wistar rats (150-200g) were divided into four groups (n = 8). Group A received normal saline only as placebo; Group B received 0.5% cuprizone diet only; Group C received a combination of 0.5% cuprizone diet and 100 mg/kg bw of DRLC and Group D received 100 mg/kg bw of DRLC only. The administration was done through oral gavage once daily for 45 days. After the last treatment, neurobehavioral tests (Morris Water Maze and Y maze) was conducted; animals sacrificed and brain harvested for histological analysis and biochemical estimations of levels of antioxidants, oxidative stress markers, neurotransmitters and enzyme activitties.


RESULTS
The results showed significant memory decline, hippocampal alterations, decrease levels of antioxidant markers, enzyme and neurotransmitters activities with concomitant increase in norepinephrine and oxidative stress markers in cuprizone induced rats relative to normal but was attenuated with DRLC administration.


CONCLUSION
Cuprizone causes cognitive impairment and neurodegeneration through oxidative stress; however, administration of DRLC ameliorated neuropathological alteration induced by cuprizone.
['Ogunlade|B|B|', 'Fidelis|O P|OP|', 'Afolayan|O O|OO|', 'Agie|J A|JA|']
D000544:Alzheimer Disease D000818:Animals D000975:Antioxidants D002374:Catalase D003471:Cuprizone D003545:Cysteine D004032:Diet D019587:Dietary Supplements D005506:Food Contamination D005981:Glutathione Synthase D006624:Hippocampus D008297:Male D008315:Malondialdehyde D009569:Nitric Oxide D018384:Oxidative Stress D051381:Rats D017208:Rats, Wistar D013482:Superoxide Dismutase D053778:Thiazolidines
D-ribose-L-cysteine Cuprizone Oxidative stress Memory and hippocampus