Effect of recombinant serine protease from adult stage of Trichinella spiralis on TNBS-induced experimental colitis in mice.
Inflammatory bowel disease (IBD), which includes ulcerative colitis (UC) and Crohn's disease (CD), is a chronic autoimmune disease. At present, worms and their products has been shown to have protective effects on immune-mediated diseases. Therefore, we aimed to investigate the effect of the recombination Trichinella spiralis (T. spiralis, Ts) adult serine protease-like protein rTs-ADSp-7 on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD mouse model. Colitis was induced by intrarectal administration of a TNBS solution. The disease activity index (DAI), which included weight loss, diarrhoea, and bloody stool, was measured. Colon segments were stained with haematoxylin and eosin (H.E.) for histopathological score. Cytokine release in the serum was analysed by meso scale discovery (MSD). Cytokine release in the colon was detected by ELISA. Splenocytes were separated, and the cytokine profiles of Th1 (IFN-γ), Th2 (IL-4), Th17 (IL-17A) and Treg cells were analysed by flow cytometry. Our result showed that rTs-ADSp-7 reduced the clinical disease activity of TNBS-induced colitis in mice. In addition, we found that rTs-ADSp-7 reduced the production of Th1- and Th17-related cytokines while upregulating the expression of Th2- and Treg-related cytokines in TNBS-induced colitis mice. rTs-ADSp-7 also increased the population of Th2 and Treg cells in TNBS-induced colitis mice. rTs-ADSp-7 alleviated the severity of TNBS-induced colitis while balancing the CD4+ T cell immune response. rTs-ADSp-7 has therapeutic potential for colitis treatment and can be used as a helminth-derived protein therapy for CD or other Th1 immunity-mediated diseases.
['Pang|Jianda|J|', 'Ding|Jing|J|', 'Zhang|Lixiao|L|', 'Zhang|Yuanyuan|Y|', 'Yang|Yaming|Y|', 'Bai|Xue|X|', 'Liu|Xiaolei|X|', 'Jin|Xuemin|X|', 'Guo|Heng|H|', 'Yang|Yong|Y|', 'Liu|Mingyuan|M|']
D000375:Aging D000818:Animals D003092:Colitis D003106:Colon D003424:Crohn Disease D016207:Cytokines D004195:Disease Models, Animal D005260:Female D015801:Helminth Proteins D008807:Mice, Inbred BALB C D011994:Recombinant Proteins D057057:Serine Proteases D013154:Spleen D050378:T-Lymphocytes, Regulatory D018417:Th1 Cells D058504:Th17 Cells D018418:Th2 Cells D017160:Trichinella spiralis D014302:Trinitrobenzenesulfonic Acid
Trichinella spiralis Th2 TNBS-induced colitis Adult serine protease Regulatory T cell