In vitro response of human osteoblasts to multi-step sol-gel derived bioactive glass nanoparticles for bone tissue engineering.
A multi-step sol-gel process was employed to synthesize bioactive glass (BG) nanoparticles. Transmission electron microscopy (TEM) revealed that the BG nanoparticles were spherical and ranged from 30 to 60 nm in diameter. In vitro reactivity of the BG nanoparticles was tested in phosphate buffer saline (PBS), Tris-buffer (TRIS), simulated body fluid (SBF), and Dulbecco's modified Eagle's medium (DMEM), in comparison with similar sized hydroxyapatite (HA) and silicon substituted HA (SiHA) nanoparticles. Bioactivity of the BG nanoparticles was confirmed through Fourier transform infrared spectroscopy (FTIR) analysis. It was found that bone-like apatite was formed after immersion in SBF at 7 days. Solutions containing BG nanoparticles were slightly more alkaline than HA and SiHA, suggesting that a more rapid apatite formation on BG was related to solution-mediated dissolution. Primary human osteoblast (HOB) cell model was used to evaluate biological responses to BG nanoparticles. Lactate dehydrogenase (LDH) cytotoxicity assay showed that HOB cells were not adversely affected by the BG nanoparticles throughout the 7day test period. Interestingly, MTS assay results showed an enhancement in cell proliferation in the presence of BG when compared to HA and SiHA nanoparticles. Particularly, statistically significant (p<0.05) alkaline phosphatase (ALP) activity of HOB cells was found on the culture containing BG nanoparticles, suggesting that the cell differentiation might be promoted by BG. Real-time quantitative PCR analysis (qPCR) further confirmed this finding, as a significantly higher level of RUNX2 gene expression was recorded on the cells cultured in the presence of BG nanoparticles when compared to those with HA and SiHA.
['Fan|Jian Ping|JP|', 'Kalia|Priya|P|', 'Di Silvio|Lucy|L|', 'Huang|Jie|J|']
D000469:Alkaline Phosphatase D001842:Bone and Bones D016923:Cell Death D049109:Cell Proliferation D048430:Cell Shape D017886:Durapatite D005898:Glass D006801:Humans D006863:Hydrogen-Ion Concentration D053758:Nanoparticles D010006:Osteoblasts D010012:Osteogenesis D010316:Particle Size D044367:Phase Transition D060888:Real-Time Polymerase Chain Reaction D012825:Silicon D012996:Solutions D013052:Spectrometry, X-Ray Emission D017550:Spectroscopy, Fourier Transform Infrared D023822:Tissue Engineering D014961:X-Ray Diffraction
Osteoblast Bioactive glass Nanoparticles Tissue engineering Hydroxyapatite Sol–gel