Simultaneous targeted analysis of trimethylamine-N-oxide, choline, betaine, and carnitine by high performance liquid chromatography tandem mass spectrometry.
Trimethylamine-N-oxide (TMAO) is a metabolite generated from choline, betaine and carnitine in a gut microbiota-dependent way. This molecule is associated with development of atherosclerosis and cardiovascular events. A sensitive liquid chromatographic electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) has been developed and validated for the simultaneous determination of TMAO related molecules including TMAO, betaine, choline, and carnitine in mouse plasma. Analytes are extracted after protein precipitation by methanol and subjected to LC-ESI-MS/MS without preliminary derivatization. Separation of analytes was achieved on an amide column with acetonitrile-water as the mobile phase. This method has been fully validated in this study in terms of selectivity, linearity, sensitivity, precision, accuracy, and carryover effect, and the stability of the analyte under various conditions has been confirmed. This developed method has successfully been applied to plasma samples of our mouse model.
['Liu|Jia|J|', 'Zhao|Mingming|M|', 'Zhou|Juntuo|J|', 'Liu|Changjie|C|', 'Zheng|Lemin|L|', 'Yin|Yuxin|Y|']
D000818:Animals D001622:Betaine D002331:Carnitine D002794:Choline D002851:Chromatography, High Pressure Liquid D008297:Male D008744:Methylamines D051379:Mice D008810:Mice, Inbred C57BL D053719:Tandem Mass Spectrometry
Choline Betaine Carnitine TMAO HPLC-ESI-MS/MS