Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association.
Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1/POT1. We show that the fission yeast TPP1 ortholog, Tpz1, is SUMOylated. Tpz1 SUMOylation restricts telomere elongation and promotes Stn1/Ten1 telomere association, and a SUMO-Tpz1 fusion protein has increased affinity for Stn1. Our data suggest that SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1, highlighting the evolutionary conservation of the regulation of CST function by SUMOylation.
['Garg|Mansi|M|', 'Gurung|Resham L|RL|', 'Mansoubi|Sahar|S|', 'Ahmed|Jubed O|JO|', 'Davé|Anoushka|A|', 'Watts|Felicity Z|FZ|', 'Bianchi|Alessandro|A|']
D000595:Amino Acid Sequence D002352:Carrier Proteins D004268:DNA-Binding Proteins D019143:Evolution, Molecular D008969:Molecular Sequence Data D011485:Protein Binding D025842:SUMO-1 Protein D012568:Schizosaccharomyces D029702:Schizosaccharomyces pombe Proteins D058207:Sumoylation D019098:Telomerase D016615:Telomere D059505:Telomere Homeostasis D034501:Telomere-Binding Proteins D000091346:Tripeptidyl-Peptidase 1
Stn1 telomeres CST Tpz1 SUMO