Toxicity and Antiviral Activity of the Extracts of Submerged Mycelium of Nematophagous Duddingtonia flagrans Fungus in Vero Cell Culture.
We studied toxicity and antiviral activity of aqueous and ethanol extracts of bioactive substances from the biomass of nematophagous fungus Duddingtonia flagrans prepared by submerged culturing of the mycelium. It is found that both extracts were characterized by low toxicity for cultured Vero cells and inhibited reproduction of DNA-viruses in this cell line. Ethanol extract of the fungus exhibited higher in vitro antiviral activity against Herpes simplex virus type 2, ectromelia virus, and vaccinia virus than water extract, which can be due to higher content of proteins, polysaccharides, flavonols, catechins, or carotenes or more effective their combination. The extracts of cultured mycelium of Duddingtonia flagrans fungus containing a complex of bioactive substances can be used for creation of broad-spectrum antiviral drugs against DNA-viruses.
['Ibragimova|Zh B|ZhB|', "Anan'ko|G G|GG|", 'Kostina|N E|NE|', 'Teplyakova|T V|TV|', 'Mazurkova|N A|NA|']
D000818:Animals D002522:Chlorocebus aethiops D059586:Duddingtonia D004481:Ectromelia virus D025282:Mycelium D018139:Simplexvirus D014616:Vaccinia virus D014709:Vero Cells
herpes simplex virus nematophagous fungus extract orthopoxviruses