Bioconversion of essential oil components of Perilla frutescens by Saccharomyces cerevisiae.
The essential oil of perilla (Perilla frutescens) contains volatile low molecular weight compounds such as monoterpenes and phenylpropenes. The composition of the essential oil is classified into about ten chemotypes. The biosynthesis of these constituents is strictly controlled genetically. Among the compounds contained in perilla essential oil, the bioconversion of pure compounds such as perillaldehyde, limonene, and citral has been reported, but that of many other components has not. In addition, changes in the volatile components of raw plant material during brewing have also been investigated for wine and beer. In this study, we examined the bioconversion of perilla essential oil components by Saccharomyces cerevisiae during the brewing of liquor with perilla leaves. S. cerevisiae was added to the ethanol-water extract of dried leaves of P. frutescens and P. citriodora for seven essential oil types: perillaldehyde type, piperitenone type, perillene type, perillaketone type, elsholtziaketone type, citral type, and phenylpropanoid type. Volatile compounds in the reaction mixtures were analyzed by solid-phase microextraction (SPME)-GC-MS, revealing bioconversion of perillaldehyde, isoegomaketone, neral, and geranial by S. cerevisiae. Analysis of the conversion products suggests that they were formed by the reduction of C=C bonds and aldehydes, as well as by esterification and dehydration reactions.
['Kimura|Michiru|M|', 'Ito|Michiho|M|']
D000080462:Acyclic Monoterpenes D000434:Alcoholic Beverages D005663:Furans D008401:Gas Chromatography-Mass Spectrometry D007659:Ketones D039821:Monoterpenes D009822:Oils, Volatile D031344:Perilla frutescens D018515:Plant Leaves D010938:Plant Oils D012441:Saccharomyces cerevisiae D017962:alpha-Linolenic Acid
Bioconversion Perilla frutescens Saccharomyces cerevisiae Monoterpenoids Perillyl alcohol Trans-shisool