Overexpression of RACK1 enhanced the replication of porcine reproductive and respiratory syndrome virus in Marc-145 cells and promoted the NF-κB activation via upregulating the expression and phosphorylation of TRAF2.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative pathogen for porcine reproductive and respiratory syndrome (PRRS), which lead to huge loss to porcine industry. RACK1 (receptor of activated protein C kinase 1) was first identified as a receptor for protein kinase C. Mounting evidence demonstrated that RACK1 played diverse roles in NF-κB activation and virus infections. We previously reported that siRNA knockdown of RACK1 inhibited PRRSV replication in Marc-145 cells, abrogated NF-κB activation induced by PRRSV infection and reduced the viral titer. Here we established a Marc-145 cell line which could stably overexpress RACK1 to consolidate our findings. Based on the data from RT-qPCR, western blot, immunofluorescence staining, cytopathic effects and viral titer analysis, we concluded that overexpression of RACK1 could enhance the replication of PRRSV in Marc-145 cells and promote the NF-κB activation via upregulating TRAF2 expression and its phosphorylation. Marc-145 cells overexpressing RACK1exhibited severe cytopathic effects post infection with PRRSV and elevated the viral titer. Taken together, RACK1 plays an essential role for PRRSV replication in Marc-145 cells and NF-κB activation. The results presented here shed more light on the understanding of the molecular mechanisms underlying PRRSV infection and its subsequent NF-κB activation. Therefore, we anticipate RACK1 as a promising target for PRRS control.
['Liu|Xiao|X|', 'Bi|Junlong|J|', 'Zhao|Qian|Q|', 'Li|Miao|M|', 'Zuo|Qingwei|Q|', 'Wang|Xiaochun|X|', 'Lan|Rui|R|', 'Li|Xidan|X|', 'Yang|Guishu|G|', 'Liu|Jianping|J|', 'Yin|Gefen|G|']
D000818:Animals D002460:Cell Line D005786:Gene Expression Regulation D008253:Macaca mulatta D016328:NF-kappa B D010766:Phosphorylation D019316:Porcine respiratory and reproductive syndrome virus D011494:Protein Kinases D000075370:Receptors for Activated C Kinase D015398:Signal Transduction D047992:TNF Receptor-Associated Factor 2 D015533:Transcriptional Activation D015854:Up-Regulation D014779:Virus Replication
Overexpression RACK1 Replication Marc-145 NF-κB TRAF2 Porcine reproductive and respiratory syndrome virus (PRRSV)