Chronic stress enhanced fear memories are associated with increased amygdala zif268 mRNA expression and are resistant to reconsolidation.
The chronically stressed brain may present a vulnerability to develop maladaptive fear-related behaviors in response to a traumatic event. In rodents, chronic stress leads to amygdala hyperresponsivity and dendritic hypertrophy and produces a post traumatic stress disorder (PTSD)-like phenotype that includes exaggerated fear learning following Pavlovian fear conditioning and resistance to extinction. It is unknown whether chronic stress-induced enhanced fear memories are vulnerable to disruption via reconsolidation blockade, as a novel therapeutic approach for attenuating exaggerated fear memories. We used a chronic stress procedure in a rat model (wire mesh restraint for 6h/d/21d) to create a vulnerable brain that leads to a PTSD-like phenotype. We then examined freezing behavior during acquisition, reactivation and after post-reactivation rapamycin administration (i.p., 40mg/kg) in a Pavlovian fear conditioning paradigm to determine its effects on reconsolidation as well as the subsequent functional activation of limbic structures using zif268 mRNA. Chronic stress increased amygdala zif268 mRNA during fear memory retrieval at reactivation. Moreover, these enhanced fear memories were unaffected by post reactivation rapamycin to disrupt long-term fear memory. Also, post-reactivation long term memory processing was also associated with increased amygdala (LA and BA), and decreased hippocampal CA1 zif268 mRNA expression. These results suggest potential challenges for reconsolidation blockade as an effective approach in treating exaggerated fear memories, as in PTSD. Our findings also support chronic stress manipulations combined with fear conditioning as a useful preclinical approach to study a PTSD-like phenotype.
['Hoffman|Ann N|AN|', 'Parga|Alejandro|A|', 'Paode|Pooja R|PR|', 'Watterson|Lucas R|LR|', 'Nikulina|Ella M|EM|', 'Hammer|Ronald P|RP|', 'Conrad|Cheryl D|CD|']
D000679:Amygdala D000818:Animals D003214:Conditioning, Classical D051766:Early Growth Response Protein 1 D005239:Fear D017403:In Situ Hybridization D008297:Male D008568:Memory D000069077:Memory Consolidation D012333:RNA, Messenger D051381:Rats D017207:Rats, Sprague-Dawley D013315:Stress, Psychological
Chronic stress Amygdala Reconsolidation Fear conditioning Rapamycin PTSD Hippocampus mTOR