MHJ_0461 is a multifunctional leucine aminopeptidase on the surface of Mycoplasma hyopneumoniae.
Aminopeptidases are part of the arsenal of virulence factors produced by bacterial pathogens that inactivate host immune peptides. Mycoplasma hyopneumoniae is a genome-reduced pathogen of swine that lacks the genetic repertoire to synthesize amino acids and relies on the host for availability of amino acids for growth. M. hyopneumoniae recruits plasmin(ogen) onto its cell surface via the P97 and P102 adhesins and the glutamyl aminopeptidase MHJ_0125. Plasmin plays an important role in regulating the inflammatory response in the lungs of pigs infected with M. hyopneumoniae. We show that recombinant MHJ_0461 (rMHJ_0461) functions as a leucine aminopeptidase (LAP) with broad substrate specificity for leucine, alanine, phenylalanine, methionine and arginine and that MHJ_0461 resides on the surface of M. hyopneumoniae. rMHJ_0461 also binds heparin, plasminogen and foreign DNA. Plasminogen bound to rMHJ_0461 was readily converted to plasmin in the presence of tPA. Computational modelling identified putative DNA and heparin-binding motifs on solvent-exposed sites around a large pore on the LAP hexamer. We conclude that MHJ_0461 is a LAP that moonlights as a multifunctional adhesin on the cell surface of M. hyopneumoniae.
['Jarocki|Veronica M|VM|', 'Santos|Jerran|J|', 'Tacchi|Jessica L|JL|', 'Raymond|Benjamin B A|BB|', 'Deutscher|Ania T|AT|', 'Jenkins|Cheryl|C|', 'Padula|Matthew P|MP|', 'Djordjevic|Steven P|SP|']
D000595:Amino Acid Sequence D000596:Amino Acids D001426:Bacterial Proteins D001665:Binding Sites D006493:Heparin D007931:Leucyl Aminopeptidase D008565:Membrane Proteins D008969:Molecular Sequence Data D045705:Mycoplasma hyopneumoniae D010958:Plasminogen D011485:Protein Binding D013379:Substrate Specificity
leucine aminopeptidase adhesin DNA-binding protein heparin-binding protein moonlighting protein plasminogen-binding protein