Preparation, structural analysis and antioxidant activities of phosphorylated (1 → 3)-β-d-glucan.
The aim of this study was to investigate the effect of phosphorylation on the antioxidant activity of (1 → 3)-β-d-glucan from yeast cell wall. Alkali-insoluble (1 → 3)-β-d-glucan was extracted from yeast cell wall by an acid-base method. It was found that the purity of the sample was greatly improved after the precipitation was treated with alkali at 90 ℃ and then by acetic acid, which was about 96.5%. Phosphorylated (1 → 3)-β-d-glucan was prepared. Infrared (IR) spectra and nuclear magnetic resonance spectra (NMR) confirmed the successful introduction of phosphate into glucan. The substitution degree of phosphate was 0.18. The phosphorylated (1 → 3)-β-d-glucan could significantly increase SOD and CAT contents in serum, liver and brain of mice, and reduce MDA level in serum, liver and brain to a certain extent in vivo. This lays a solid foundation for the research and development of phosphorylated (1 → 3)-β-d-glucan antioxidant.
['Mei|Xinya|X|', 'Tang|Qilin|Q|', 'Huang|Gangliang|G|', 'Long|Rong|R|', 'Huang|Hualiang|H|']
D000818:Animals D000975:Antioxidants D002374:Catalase D002473:Cell Wall D009682:Magnetic Resonance Spectroscopy D008315:Malondialdehyde D051379:Mice D010766:Phosphorylation D012441:Saccharomyces cerevisiae D013055:Spectrophotometry, Infrared D013482:Superoxide Dismutase D047071:beta-Glucans
Preparation Antioxidant activities Yeast cell wall Phosphorylated (1→3)-β-d-glucan Structural characterization