Effects of dietary white mulberry leaves on hemato-biochemical alterations, immunosuppression and oxidative stress induced by Aeromonas hydrophila in Oreochromis niloticus.
The present work was designed to assess the potential hemato-biochemical protective action, immunemodulatory and antioxidant conclusions of varied concentration of white mulberry Morus alba leaves (MAL) extract supplementation on Nile tilapia (Oreochromis .niloticus). A total two hundred and forty of O. niloticus were haphazardly sorted into four groups. The control (CT) group was fed on basal diet. A group MAL1, MAL3 and MAL5 was fed on 1, 3 and 5 g/kg MAL respectively for thirty days. On day thirty one, half of replicates in each group were challenged by 0.5 ml × 108Aeromonas hydrophila where, the residual replicates were kept without challenge. A. hydrophila challenged tilapias revealed anemia that alleviated by supplementation with 5 g/kg MAL also, recovers the shift of leucogram prompted by the challenge. Elevation of alkaline phosphatase, aminotransferases, lactate dehydrogenase and malondialdehyde (ALP, ALT, AST, LDH and MDA) in CT, MAL1 and MAL3 in the challenged replicates respectively where within normal at MAL5. Supplementation with MAL5 showed more potent antioxidant and immune reaction than MAL1 and MAL3. There were a rapid increase of immunoglobulin M, lysozymes, nitric oxide, catalase and superoxide dismutase and their allied genes expression (IgM, CAT and SOD) in MAL groups with contrast in CT challenged groups. Where in challenged groups, there was suppression in genes expression of interleukins (8 and 1 beta) and interferon ɤ (IL8. IL-1β and INFɤ). Tilapias challenged by A. hydrophila unveiled plentiful surge in the percentage of mortality in CT challenged fish (80%), followed by the groups supplemented with MAL1 and MAL3 were (73.33%) where MAL5 was 20%. The mortalities have been halted from the 6th, 13th, 14th and 15th days in, MAL5, MAL3, MAL1, and CT correspondingly. These previous results could be fulfilled that using of MAL 5 g/kg protect tilapias from hemato-biochemical alterations and enhance its immune feedback, antioxidant defense and resistance against A. hydrophila.
['Neamat-Allah|Ahmed N F|ANF|', 'Mahmoud|Essam A|EA|', 'Mahsoub|Y|Y|']
D016980:Aeromonas hydrophila D000821:Animal Feed D000818:Animals D001774:Blood Chemical Analysis D023681:Cichlids D004032:Diet D019587:Dietary Supplements D004305:Dose-Response Relationship, Drug D005393:Fish Diseases D016905:Gram-Negative Bacterial Infections D007108:Immune Tolerance D031625:Morus D018384:Oxidative Stress D010936:Plant Extracts D018515:Plant Leaves D011897:Random Allocation
Mulberry Morus alba MAL Nile tilapia Cytokines Hematology Hydrophila spp Immune response