Overexpression of activating transcription factor 3 exerts suppressive effects in HepG2 cells.
The present study observed and compared the biological behaviour of HepG2 cells prior and subsequent to the overexpression of activating transcription factor 3 (ATF3). Experiments investigating the cytological function by which ATF3 affects liver cancer cells were also performed. MTT, Transwell and flow cytometry assays were used to observe and detect the biological behaviour of HepG2 cells with and without lentivirus (LV)‑ATF3‑enhanced green fluorescent protein (EGFP) infection. The effects of ATF3 overexpression on cell proliferation, migration, apoptosis and cell cycle progression were evaluated. The LV‑ATF3‑EGFP overexpression vector was successfully constructed, and the HepG2 cells were successfully infected with the vector. Following ATF3 overexpression, cell proliferation was decreased, the rate of cell apoptosis was accelerated and cell cycle progression was slowed (P<0.05). There were no marked changes in cell migration (P>0.05), although there was a trend towards a gradual decrease. In conclusion, ATF3 exerted suppressive effects in HepG2 cells, potentially by inhibiting cancer cell growth, accelerating cell apoptosis, and blocking cell cycle progression. Intervention targeting ATF3 expression may represent a novel approach for the prevention and treatment of human liver cancer.
['Li|Xiaoyan|X|', 'Zang|Shengbing|S|', 'Cheng|Haili|H|', 'Li|Jiasi|J|', 'Huang|Aimin|A|']
D051700:Activating Transcription Factor 3 D017209:Apoptosis D002453:Cell Cycle D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D015972:Gene Expression Regulation, Neoplastic D056945:Hep G2 Cells D006801:Humans D008113:Liver Neoplasms
activating  transcription  factor  3 lentiviral  vectors  of  overexpression cytological mechanism