Efficacy of UV-C photolysis of bisphenol A on transcriptome alterations of genes in zebrafish embryos.
The purpose of this study was to investigate the efficacy of UV-C direct photolysis of bisphenol A (BPA) as a remediation method of BPA contamination. We used zebrafish embryos as a model organism to test the toxicity and residual biological activity by measuring cytochrome P4501A1 (CYP1A), aromatase B (Aro B) and heat shock proteins (HSP-70) transcript levels. The mRNA levels of CYP1A gene increased about two fold while exposure of zebrafish embryos at 72 hpf resulted in significant induction (P = 0.048) of Aro B at 100 µg/L of BPA. Exposure of zebrafish embryos at 72 hpf to increasing concentrations of BPA resulted in significant induction (P = 0.0031) of HSP-70 transcript level. UV treatment of BPA resulted in a significant reduction in toxicity by reducing mortality of zebrafish embryos. The results suggest that UV-C direct photolysis may be an effective method for remediation of BPA contamination. Further studies will be necessary for better understanding of the identity and relative activity of the UV degradation by-products.
['Saeed|Asma|A|', 'Hashmi|Imran|I|', 'Zare|Ava|A|', 'Mehrabani-Zeinabad|Mitra|M|', 'Achari|Gopal|G|', 'Habibi|Hamid R|HR|']
D000818:Animals D001141:Aromatase D001559:Benzhydryl Compounds D019363:Cytochrome P-450 CYP1A1 D004625:Embryo, Nonmammalian D005819:Genetic Markers D010636:Phenols D010782:Photolysis D059467:Transcriptome D014466:Ultraviolet Rays D015027:Zebrafish D029961:Zebrafish Proteins
Bisphenol A zebrafish embryos QPCR UV photolysis biomarker genes endocrine disruption