Keratin 8 is a novel autoantigen of rheumatoid arthritis.
OBJECTIVE
This research aims to verify Keratin 8 (K8) as a specific autoantigen in rheumatoid arthritis (RA).


METHODS
First, total RNA was extracted from HaCaT cell to obtain cDNA by inverse transcription. Then, PCR was performed to amplify corresponding gene by K8 primers. Next, cloning, expression, and purification technology were used to obtain the recombinant human K8 (rhK8). At last, the purified rhK8, after identified by mass spectrometer, was used to perform further disease-related Western blotting and ELISA test with real clinical samples.


RESULTS
Purified rhK8 was successfully obtained and then Western blotting confirmed antigenicity of K8 in rheumatoid arthritis. The reactivity of serum IgG against rhK8 was further detected in 34 of 50 RA patients (68%). The reactivity of RA serum IgG antibodies against K8 was significantly higher than healthy controls and systemic lupus erythematosus (SLE) patients.


CONCLUSION
This research confirmed Keratin 8 as a novel autoantigen of RA.
['Wang|Xiaoxu|X|', 'Chen|Peng|P|', 'Cui|Jiawen|J|', 'Yang|Chunhe|C|', 'Du|Hongwu|H|']
D000328:Adult D000368:Aged D000595:Amino Acid Sequence D001172:Arthritis, Rheumatoid D001323:Autoantibodies D001324:Autoantigens D015153:Blotting, Western D016022:Case-Control Studies D004797:Enzyme-Linked Immunosorbent Assay D000939:Epitopes D005260:Female D006801:Humans D007074:Immunoglobulin G D053533:Keratin-8 D008180:Lupus Erythematosus, Systemic D008297:Male D008875:Middle Aged D008969:Molecular Sequence Data D011994:Recombinant Proteins D055815:Young Adult
Keratin 8 Autoantigen Rheumatoid arthritis