Use of mixed culture bacteria for photofermentive hydrogen of dark fermentation effluent.
Hydrogen production (HP) from dark fermentation effluent of starch wastewater via vertical tubular photo-bioreactor was investigated. The reactor was inoculated with mixed culture of bacteria and operated at light intensity of 190 W/m(2). Hydraulic retention time (HRT) and organic loading rate (OLR) was varied between 0.9 to 4.0 h and 3.2 to 16 g COD/l.d., respectively. Increasing the HRT from 0.9 to 2.5 h, significantly (P<0.05) increased HP from 1±0.04 to 3.05±0.19 l/d, respectively. However, minimal increase in HP occurred when increasing the HRT up to 4.0 h. The HP remained unaffected when increasing the OLR from 3.2 to 6.4 g COD/l.d. Further increase in the OLR up to 8.2 and 16 g COD/l.d., resulted in a drop in HP i.e. 0.96 and 0.19 l/d, respectively. Microbial community analysis of the reactor samples showed the presence and dominance of hydrogen producing purple non-sulfur phototrophic (PNS) bacterium, Rhodopseudomonas palustris in the reactor.
['Tawfik|Ahmed|A|', 'El-Bery|Haitham|H|', 'Kumari|Sheena|S|', 'Bux|Faizal|F|']
D064751:Ammonium Compounds D001419:Bacteria D057919:Biological Oxygen Demand Analysis D002264:Carboxylic Acids D003624:Darkness D058645:Denaturing Gradient Gel Electrophoresis D005232:Fatty Acids, Volatile D005285:Fermentation D006859:Hydrogen D058433:Photobioreactors D010802:Phylogeny D016133:Polymerase Chain Reaction D013997:Time Factors D014865:Waste Disposal, Fluid
Vertical tubular photo-bioreactor HRT Bio-hydrogen Microbial analysis Photo-fermentation