Determination and pharmacokinetic analysis of ticarcillin disodium-clavulanate potassium for injection in rat plasma by UPLC-ESI-MS/MS.
OBJECTIVE
To establish a specific and rapid ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method for measuring ticarcillin and clavulanate levels in rat plasma.


METHODS
A Waters ACQUITY BEH C18 column (50 mm × 2.1 mm, 1.7 μm) and SCIEX QTRAP® LC-MS/MS System were used. Analyses were conducted to optimize the chromatographic and MS conditions, and the pharmacokinetic parameters of ticarcillin and clavulanate were assessed.


RESULTS
Linear relationships were observed in the ranges of 10 to 10,000 ng/mL for ticarcillin R (r2 = 0.9967) 30 to 10,000 ng/mL for ticarcillin S (r2 = 0.9961), and 30 to 10,000 ng/mL for clavulanate (r2 = 0.9981). The average extraction recoveries of all compounds ranged from 86.9% to 96.4%. The pharmacokinetic parameters of the ticarcillin R and S isomers in rats were distinctive. The ticarcillin R and S isomers and clavulanate were rapidly absorbed in vivo. Ticarcillin S and clavulanate had similar elimination rates, whereas that of ticarcillin R was slower.


CONCLUSION
A UPLC-ESI-MS/MS method was developed and validated for the determination of ticarcillin and clavulanate in rat plasma.
['Wang|Moli|M|', 'Gao|Yanxia|Y|', 'Liu|Xueli|X|', 'Zhang|Jing|J|', 'Wang|Qiang|Q|', 'Chang|Junshan|J|', 'Zhang|Lantong|L|https://orcid.org/0000-0003-1765-2948']
D000284:Administration, Oral D000818:Animals D002853:Chromatography, Liquid D019818:Clavulanic Acid D051381:Rats D017207:Rats, Sprague-Dawley D015203:Reproducibility of Results D053719:Tandem Mass Spectrometry D013982:Ticarcillin
pharmacokinetics ticarcillin rat mass spectrometry in vivo Clavulanate potassium ultra-high-performance liquid chromatography