A study on the interaction between 3-spiro-piperidones and bovine serum albumin using spectroscopic approaches.
The interaction between 3-spiro-2'-pyrrolidine-3'-spiro-3″-piperidine-2,3″-dione (PPD) and bovine serum albumin (BSA) in aqueous solution was studied using fluorescence and UV-vis spectroscopy. Fluorescence emission data revealed that BSA (1.00 × 10(-5)  mol/L) fluorescence was statically quenched by PPD at various concentrations, which implies that a PPD-BSA complex was formed. The binding constant (KA ), the number of binding sites (n) and the specific binding site of the PPD with BSA were determined. Energy-transfer efficiency parameters were determined and the mechanism of the interaction discussed. The thermodynamic parameters, ΔG, ΔH and ΔS, were obtained according to van't Hoff's equation, showing the involvement of hydrophobic forces in these interactions. The effect of PPD acting on the BSA conformation was detected by synchronous fluorescence.
['Yu|Xianyong|X|', 'Yao|Qing|Q|', 'Tao|Hongwen|H|', 'Yang|Ying|Y|', 'Li|Lei|L|', 'Zheng|Baishu|B|', 'Zhu|Shizhong|S|']
D000818:Animals D002417:Cattle D005453:Fluorescence D010881:Piperidones D011760:Pyrrolidinones D012710:Serum Albumin, Bovine D013050:Spectrometry, Fluorescence D013056:Spectrophotometry, Ultraviolet D013816:Thermodynamics
interaction bovine serum albumin 3′-spiro-3″-piperidine-2,3″-dione fluorescence spectroscopy ultraviolet spectroscopy