Peptidomic changes of saliva after nonsurgical treatment of stage I / II generalized periodontitis.
OBJECTIVE
To explore the changes of peptidome profiles of saliva, serum and gingival crevicular fluid (GCF) before and after nonsurgical periodontal treatment in patients with generalized periodontitis (stage I / II).


SUBJECTS AND METHODS
Saliva, serum and GCF samples were collected from 17 patients at baseline (T0 ), one week after ultrasonic supragingival scaling (T1 ) and eight weeks after subgingival scaling and root planning (T2 ). Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) was carried out to detect changes in peptidomic profiles. Then nano-liquid chromatography-electrospray ionization-tandem mass spectrometry (nano-LC/ESI-MS/MS) was performed to identify potential peptide biomarkers.


RESULTS
Most of the peptides from the patients exhibited a decreasing trend from the time point of pre-treatment to that of post-treatment. Cluster analysis and scatter plots using these peptides indicated that salivary peptidome has an acceptable capability of reflecting the status of stage I / II generalized periodontitis. Seven of these peptides were successfully identified as α-1-antitrypsin, immunoglobulin κ variable 4-1, haptoglobin and immunoglobulin heavy constant γ2.


CONCLUSIONS
Certain peptides in saliva, serum and GCF were down-regulated after nonsurgical periodontal treatment, demonstrating the application prospects of saliva in monitoring and surveillance of periodontal diseases in both clinical settings and communities.
['Yuan|Chao|C|', 'Ma|Zhangke|Z|', 'Tong|Peiyuan|P|', 'Yu|Shunlan|S|', 'Li|Yi|Y|', 'Elizabeth Gallagher|Jennifer|J|', 'Sun|Xiangyu|X|', 'Zheng|Shuguo|S|https://orcid.org/0000-0001-6717-5196']
saliva nonsurgical treatment mass spectrometry chronic periodontitis