CytoMatrix for a reliable and simple characterization of lung cancer stem cells from malignant pleural effusions.
Cancer stem cells (CSCs) are a subpopulation with the properties of extensive self-renewal, capability to generate differentiated cancer cells and resistance to therapies. We have previously shown that malignant pleural effusions (MPEs) from patients with non-small-cell lung cancer (NSCLC) represent a valuable source of cancer cells that can be grown as three-dimensional (3D) spheroids enriched for stem-like features, which depend on the activation of the Yes-associated protein-transcriptional coactivator with PDZ-binding motif (YAP-TAZ)/Wnt-βcatenin/stearoyl-CoA desaturase 1 (SCD1) axis. Here, we describe a novel support, called CytoMatrix, for the characterization of limited amounts of cancer cells isolated from MPEs of patients with NSCLC. Our results show that this synthetic matrix allows an easy and fast characterization of several epithelial cellular markers. The use of CytoMatrix to study CSCs subpopulation confirms that SCD1 protein expression is enhanced in 3D spheroids when compared with 2D adherent cell cultures. YAP/TAZ nuclear-cytoplasmic distribution analysed by CytoMatrix in 3D spheroids is highly heterogeneous and faithfully reproduces what is observed in tumour biopsies. Our results confirm and extend the robustness of our workflow for the isolation and phenotypic characterization of primary cancer cells derived from the lung MPEs and underscore the role of SCD1.
['Bruschini|Sara|S|', 'di Martino|Simona|S|', 'Pisanu|Maria Elena|ME|', 'Fattore|Luigi|L|', 'De Vitis|Claudia|C|', 'Laquintana|Valentina|V|', 'Buglioni|Simonetta|S|', 'Tabbì|Eugenio|E|', 'Cerri|Andrea|A|', 'Visca|Paolo|P|', 'Alessandrini|Gabriele|G|', 'Facciolo|Francesco|F|', 'Napoli|Christian|C|', 'Trombetta|Marcella|M|', 'Santoro|Antonio|A|', 'Crescenzi|Anna|A|', 'Ciliberto|Gennaro|G|', 'Mancini|Rita|R|0000-0002-5491-2449']
D000368:Aged D014408:Biomarkers, Tumor D002289:Carcinoma, Non-Small-Cell Lung D018929:Cell Culture Techniques D002467:Cell Nucleus D003581:Cytodiagnosis D003593:Cytoplasm D005260:Female D006801:Humans D008168:Lung D008175:Lung Neoplasms D008297:Male D008875:Middle Aged D014411:Neoplastic Stem Cells D016066:Pleural Effusion, Malignant D018874:Spheroids, Cellular D013230:Stearoyl-CoA Desaturase D014157:Transcription Factors D014407:Tumor Cells, Cultured
CytoMatrix lung cancer cancer stem cells MPEs SCD1