Assessment of the repeatability and border-plate effects of the B158/B60 enzyme-linked-immunosorbent assay for the detection of circulating antigens (Ag-ELISA) of Taenia saginata.
The monoclonal antibody-based circulating antigen detecting ELISA (B158/B60 Ag-ELISA) has been used elaborately in several studies for the diagnosis of human, bovine and porcine cysticercosis. Interpretation of test results requires a good knowledge of the test characteristics, including the repeatability and the effect of the borders of the ELISA plates. Repeatability was tested for 4 antigen-negative and 5 antigen-positive reference bovine serum samples by calculating the Percentage Coefficient of Variation (%CV) within and between plates, within and between runs, overall, for two batches of monoclonal antibodies and by 2 laboratory technicians. All CV values obtained were below 20% (except one: 24.45%), which indicates a good repeatability and a negligible technician error. The value of 24.45% for indicating the variability between batches of monoclonal antibodies for one positive sample is still acceptable for repeatability measures. Border effects were determined by calculating the %CV values between the inner and outer wells of one plate for 2 positive serum samples. Variability is a little more present in the outer wells but this effect is very small and no significant border effect was found.
['Jansen|Famke|F|', 'Dorny|Pierre|P|', 'Berkvens|Dirk|D|', 'Van Hul|Anke|A|', 'Van den Broeck|Nick|N|', 'Makay|Caroline|C|', 'Praet|Nicolas|N|', 'Gabriël|Sarah|S|']
D000818:Animals D000947:Antigens, Helminth D002417:Cattle D002418:Cattle Diseases D004797:Enzyme-Linked Immunosorbent Assay D015203:Reproducibility of Results D012680:Sensitivity and Specificity D041202:Taenia saginata D013622:Taeniasis
Repeatability Border-plate effect Ag-ELISA Taenia saginata