Bone Marrow Stem Cells-Seeded Polyethylene Terephthalate Scaffold in Repair and Regeneration of Rabbit Achilles Tendon.
The objective of this study was to evaluate the effect of bone marrow stem cells (BMSCs)-seeded polyethylene terephthalate (PET) scaffold for Achilles tendon repair in a rabbit model. The allogeneic BMSCs were seeded onto the PET scaffold and cultured in vitro for 14 days. Sixteen mature New Zealand rabbits underwent surgery to establish a 2-cm Achilles tendon defect model. The BMSCs-seeded PET scaffold was implanted into the defect of one limb (BMSCs-PET group), while the PET scaffold without BMSCs was implanted into the defect of contralateral limb as the control (PET group). All rabbits were sacrificed at 6 and 12 weeks after surgery. At 12 weeks after surgery, macroscopic and histological results showed formation of tendon-like tissues, and the structure was more mature in the BMSCs-PET group. Immunohistochemical analysis and real-time polymerase chain reaction (RT-PCR) demonstrated that the collagen I and collagen III were significantly higher in the BMSCs-PET group compared with those in the PET group. Mechanically, both the failure load and the average stiffness were significantly higher in the BMSCs-PET group than those in the PET group. In conclusion, BMSCs-seeded PET scaffold could effectively facilitate the healing process after being implanted in a rabbit Achilles tendon defect model.
['Cai|Jiangyu|J|http://orcid.org/0000-0003-4819-1160', 'Yang|Yimeng|Y|', 'Ai|Chengchong|C|http://orcid.org/0000-0003-2143-103X', 'Jin|Wenhe|W|', 'Sheng|Dandan|D|', 'Chen|Jun|J|http://orcid.org/0000-0003-4573-801X', 'Chen|Shiyi|S|']
D000125:Achilles Tendon D000818:Animals D001696:Biomechanical Phenomena D001854:Bone Marrow Cells D002478:Cells, Cultured D011093:Polyethylene Terephthalates D011817:Rabbits D012038:Regeneration D033581:Stem Cell Transplantation D013234:Stem Cells D023822:Tissue Engineering D054457:Tissue Scaffolds D014945:Wound Healing
Polyethylene terephthalate Scaffold Regeneration Achilles tendon Bone marrow stem cell