Effects of different roughage sources and feeding levels on adipogenesis of ovine adipocytes.
The objective of the present study was to conduct an adipogenic evaluation of different roughage sources and feeding levels during ruminant adipocyte differentiation in vitro. Six wether sheep were divided into a timothy hay feeding group (TFG, n = 3) and an Italian ryegrass straw feeding group (IFG, n = 3). The sheep were fed high-roughage (HR), medium roughage (MR) and low-roughage (LR) diets in a one-way layout design each over a 6-day period. Sheep serum samples collected on the last day of each dietary treatment were added to an adipogenic induction medium for differentiation of preadipocytes derived from sheep subcutaneous adipose tissue. The cytoplasmic lipid accumulations in the TFG serum-treated preadipocytes were significantly higher than those of the IFG-serum treated preadipocytes on day 12. Messenger RNA expression of CCAAT/enhancer-binding protein (C/EBP)-α, C/EBP-β, C/EBP-δ, fatty-acid-binding protein (aP2) and stearoyl-coenzyme A desaturase (SCD) were regulated by each serum treatment. This study shows that different roughage source diets and roughage-to-concentrate ratio diets can regulate adipocyte differentiation via ruminant blood composition.
['Kim|Da-Hye|DH|', 'Choi|Ki-Choon|KC|', 'Ichinohe|Toshiyoshi|T|', 'Song|Sang-Houn|SH|']
D017667:Adipocytes D050156:Adipogenesis D000273:Adipose Tissue D000821:Animal Feed D000818:Animals D002454:Cell Differentiation D002478:Cells, Cultured D003470:Culture Media D003593:Cytoplasm D004043:Dietary Fiber D050356:Lipid Metabolism D008297:Male D012756:Sheep D040521:Subcutaneous Tissue
adipocyte differentiation lipid accumulation serum treatment