Nonomuraea insulae sp. nov., isolated from forest soil.
Strain H2R21T, a novel actinobacterium, isolated from a forest soil sample collected from Heybeliada, Istanbul, Turkey, and a polyphasic approach was used for characterisation of the strain. Chemotaxonomic and morphological characterisation of strain H2R21T indicated that it belongs to the genus Nonomuraea. 16S rRNA gene sequence similarity showed that the strain is closely related to Nonomuraea purpurea 1SM4-01T (99.1%) and Nonomuraea solani CGMCC 4.7037T (98.4%). DNA-DNA relatedness values were found to be lower than 70% between the isolate and its phylogenetic neighbours N. purpurea 1SM4-01T, N. solani CGMCC 4.7037T and Nonomuraea rhizophila YIM 67092T. The whole cell hydrolysates of strain H2R21T were found to contain meso-diaminopimelic acid as the diagnostic diamino acid and glucose, madurose, mannose and ribose as the cell sugars. The polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, two glycophospholipids and two unidentified lipids. The predominant menaquinones were identified as MK-9(H4) and MK-9(H6). The major fatty acids were found to be iso-C16:0, iso-C16:0 2OH and C17:0 10-methyl. On the basis of DNA-DNA relatedness data and some phenotypic characteristics, it is evident that strain H2R21T can be distinguished from the closely related species in the genus Nonomuraea. Thus, it is concluded that strain H2R21T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea insulae sp. nov. is proposed. The type strain is H2R21T (= DSM 102915T = CGMCC 4.7338T = KCTC 39769T).
['Saricaoglu|Salih|S|', 'Nouioui|Imen|I|', 'Ay|Hilal|H|', 'Saygin|Hayrettin|H|', 'Bektas|Kadriye Inan|KI|', 'Guven|Kiymet|K|', 'Cetin|Demet|D|', 'Klenk|Hans-Peter|HP|', 'Isik|Kamil|K|', 'Sahin|Nevzat|N|http://orcid.org/0000-0002-6026-656X']
D000192:Actinomycetales D015373:Bacterial Typing Techniques D004269:DNA, Bacterial D005227:Fatty Acids D065928:Forests D010802:Phylogeny D012336:RNA, Ribosomal, 16S D012988:Soil Microbiology D014421:Turkey
Nonomuraea insulae sp. nov. 16S rRNA gene Actinobacteria Polyphasic taxonomy