An Integrated Multi-Function Heterogeneous Biochemical Circuit for High-Resolution Electrochemistry-Based Genetic Analysis.
Modular construction of an autonomous and programmable multi-functional heterogeneous biochemical circuit that can identify, transform, translate, and amplify biological signals into physicochemical signals based on logic design principles can be a powerful means for the development of a variety of biotechnologies. To explore the conceptual validity, we design a CRISPR-array-mediated primer-exchange-reaction-based biochemical circuit cascade, which probes a specific biomolecular input, transform the input into a structurally accessible form for circuit wiring, translate the input information into an arbitrary sequence, and finally amplify the prescribed sequence through autonomous formation of a signaling concatemer. This upstream biochemical circuit is further wired with a downstream electrochemical interface, delivering an integrated bioanalytical platform. We program this platform to directly analyze the genome of SARS-CoV-2 in human cell lysate, demonstrating the capability and the utility of this unique integrated system.
['Dai|Yifan|Y|0000-0002-1009-5790', 'Xu|Wei|W|', 'Somoza|Rodrigo A|RA|', 'Welter|Jean F|JF|', 'Caplan|Arnold I|AI|', 'Liu|Chung Chiun|CC|']
D015374:Biosensing Techniques D000086382:COVID-19 D064113:CRISPR-Cas Systems D002460:Cell Line D055664:Electrochemical Techniques D005814:Genes, Viral D006801:Humans D021141:Nucleic Acid Amplification Techniques D017394:RNA, Guide D000086402:SARS-CoV-2
CRISPR bioanalytical chemistry electrochemistry genetic circuits primer exchange reaction