Effect of chitosan nanoparticle, QMix, and EDTA on TotalFill BC sealers' dentinal tubule penetration: a confocal laser scanning microscopy study.
The aim of the present study was to compare the effect of chitosan nanoparticle, QMix, and 17% EDTA on the penetrability of a calcium silicate-based sealer into dentinal tubules using a confocal laser scanning microscope (CLSM). Sixty mandibular premolar teeth were selected and randomly divided into three groups (n = 20) before root canal preparation according to the solution used in the final rinse protocol: chitosan, QMix, and EDTA groups. Twenty teeth of each group were filled with a TotalFill BC sealers' single gutta-percha cone and with 0.1% rhodamine B. The specimens were horizontally sectioned at 3 and 5 mm from the apex, and the slices were analyzed in CLSM (4×). Total percentage and maximum depth of sealer penetration were measured using confocal laser scanning microscopy with using Image J analysis software. Dentinal tubule's penetration depth, percentage, and area were measured using imaging software. Kruskal-Wallis test was used for statistical analysis. The level of significance was set at 5%. Results of Kruskal-Wallis analysis showed that there was a significant difference in the percentage and depth of sealer penetration among all groups at 3 and 5 mm level sections (P < 0.05). Within the groups, the minimum sealer penetration depth was recorded for chitosan nanoparticle group. Greater depth of sealer penetration was recorded at 5 mm as compared to 3 mm in all the groups. Within the limitation of the present study, it can be concluded that QMix and EDTA promoted sealer penetration superior to that achieved by chitosan nanoparticle.
['Aydın|Zeliha Uğur|ZU|', 'Özyürek|Taha|T|http://orcid.org/0000-0003-3299-3361', 'Keskin|Büşra|B|', 'Baran|Talat|T|']
D001641:Bicuspid D001645:Biguanides D048271:Chitosan D003804:Dentin D004492:Edetic Acid D006171:Gutta-Percha D006801:Humans D007091:Image Processing, Computer-Assisted D066298:In Vitro Techniques D018613:Microscopy, Confocal D053758:Nanoparticles D011108:Polymers D012235:Rhodamines D012387:Root Canal Filling Materials D012389:Root Canal Obturation
Chitosan nanoparticle QMix EDTA Sealer penetration Confocal microscopy