A reliable phenotypic assay for detection of ESBLs and AmpCs in MBL-producing gram-negative bacteria with the use of aminophenylboronic acid, dipicolinic acid and cloxacillin.
ESBLs and AmpCs may escape detection when they coexist with metallo-β-lactamases such as New Delhi Metallo-β-lactamases-1. In this study a combination disk assay was established using cefotaxime, cefotaxime/clavulanic acid, cefotaxime/clavulanic acid/cloxacillin, cefoxitin and cefoxitin/phenylboronic acid/cloxacillin on Mueller Hinton agar supplemented with dipicolinic acid for determination of β-lactamases in the presence of NDM-1.
['Datta|Saswati|S|', 'Chatterjee|Somdatta|S|', 'Mitra|Shravani|S|', 'Basu|Sulagna|S|']
D000900:Anti-Bacterial Agents D001426:Bacterial Proteins D015373:Bacterial Typing Techniques D001897:Boronic Acids D003023:Cloxacillin D006090:Gram-Negative Bacteria D016905:Gram-Negative Bacterial Infections D006801:Humans D010641:Phenotype D010848:Picolinic Acids D001618:beta-Lactamases
Gram-negative bacteria Dipicolinic acid Cloxacillin Phenylboronic acid NDM-1 Combination disk test