In vitro selection of antibiotic-binding aptamers.
Despite its wide applicability the selection of small molecule-binding RNA aptamers with high affinity binding and specificity is still challenging. We will present here a protocol which allows the in vitro selection of antibiotic-binding aptamers which turned out to be important building blocks for the design process of synthetic riboswitches. The presented methods will be compared with alternative in vitro selection protocols. A detailed note section will point out useful tips and pitfalls.
['Groher|Florian|F|', 'Suess|Beatrix|B|']
D000900:Anti-Bacterial Agents D052157:Aptamers, Nucleotide D058928:Riboswitch D052156:SELEX Aptamer Technique
In vitro selection RNA aptamer Biotechnology Combinatorial chemistry SELEX