Suppression of microRNA-96 expression inhibits the invasion of hepatocellular             carcinoma cells.
MicroRNA-96 (miR-96) expression is dysregulated in certain types of cancers.             However, the role of miR-96 in hepatocellular carcinoma (HCC) invasion and metastasis             remains elusive. miR-96 expression was investigated in a number of HCC cell lines             by quantitative reverse transcription‑polymerase chain reaction (RT-PCR). Cell             invasive ability of metastatic HCCLM6 cells transfected with anti-miR-96 oligonucleotides             or miR-96 mimic was determined by Matrigel invasion assay in vitro. In addition,             metastasis-associated protein, osteopontin, was evaluated by Western blotting.             miR-96 expression was significantly increased in highly metastatic HCCLM6 cells.             Decreasing miR-96 expression with anti-miR-96 oligonucleotides led to reduced             migration and invasion of HCCLM6 cells in vitro. In particular, down-regulation             of miR-96 decreased osteopontin expression in HCCLM6 cells. Suppression of miR-96             expression inhibits the invasion of HCC cells, suggesting that miR-96 may be a             therapeutic target for inhibiting HCC invasion and metastasis.
['Chen|Rong-Xin|RX|', 'Xia|Yun-Hong|YH|', 'Xue|Tong-Chun|TC|', 'Ye|Sheng-Long|SL|']
D006528:Carcinoma, Hepatocellular D045744:Cell Line, Tumor D002465:Cell Movement D015536:Down-Regulation D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008113:Liver Neoplasms D035683:MicroRNAs D009361:Neoplasm Invasiveness D020319:Oligodeoxyribonucleotides, Antisense
microRNA-96 hepatocellular carcinoma osteopontin