Quantitative Method to Track Proteolytic Invasion in 3D Collagen.
Since many tumors are associated with a pronounced collagen-rich stromal reaction, there is increasing interest in understanding mechanisms by which cancer cells invade through the collagen barrier. Here we describe a quantitative method to track cell invasion in 3D collagen I gels. We analyze invasion by quantifying proteolytic tracks generated by invading cancer cells through a 3D collagen microenvironment. We provide a detailed protocol for this quantitative assay, which can be used to characterize signaling pathways that regulate invasion in the 3D microenvironment.
['Ebine|Kazumi|K|', 'Chow|Christina R|CR|', 'Munshi|Hidayatullah G|HG|']
D021441:Carcinoma, Pancreatic Ductal D018929:Cell Culture Techniques D045744:Cell Line, Tumor D002465:Cell Movement D024042:Collagen Type I D005109:Extracellular Matrix D005782:Gels D006801:Humans D053511:Matrix Metalloproteinase 14 D009361:Neoplasm Invasiveness D010190:Pancreatic Neoplasms D059748:Proteolysis D034741:RNA, Small Interfering
Invasion Proteolytic tracks 3D collagen gels MT1-MMP Proteolysis