Oral Biofilm on Dental Materials Among Khat Chewers.
BACKGROUND
Nowadays khat chewing habit is increasing among population in southern part of Saudi Arabia, Jazan and till date there is no literature investigating the effect of khat on oral biofilm on dental materials.


OBJECTIVE
To evaluate and compare the bacterial biofilm on different types of dental restorative materials used in replacing missing tooth structures among khat chewers and non-khat chewers.


MATERIALS AND METHODS
Hundred and twenty biofilm samples were collected from different dental restorations, such as All-ceramic (AL), Metal Ceramic (MC), Metal crowns or bridges (M), Composite (C), Glass Ionomer (GI) and Amalgam (A) restorations in non-khat and khat chewers (K). DNA extraction was done and subjected to PCR. Bacterial species, such as Streptococcus, Neisseria, Bacillus, Granulicatella and Veillonella were identified and counted. PCR products were also sequenced to detect similarity. Association between bacterial type and dental materials among non-khat and khat chewers were tested with Chi-Square test (Fishers Exact test).


RESULTS
The frequency and percentage of Streptococcus species were marginally higher among khat chewers (42; 70%) compared with non-khat chewers (38; 63.3%) group. But the Veillonella species were higher among non-khat chewers (9: 15%), compared to the khat chewers group (7; 11.7%). No statistically significant difference was detected among species in both groups. In non-khat and khat chewer group, the maximum hits were related to Streptococcus spp. in glass ionomer, amalgam, and composite (restorative materials), followed by metal ceramic and metal (prosthetic materials). Veillonella spp. showed maximum hits in the metal group among non-khat chewers and in all-ceramic among khat chewers. Statically significant differences were recorded among composite and amalgam samples with p values 0.047 and 0.036 in khat chewer group.


CONCLUSION
Khat chewers showed statistically significant differences in oral biofilm in the composite and amalgam restorative materials, but there were no significant differences found among any materials and species between the groups.
['Al Moaleem|Mohammed M|MM|', 'Porwal|Amit|A|', 'Al Ahmari|Nasser M|NM|', 'Shariff|Mansoor|M|']
D000293:Adolescent D000328:Adult D000368:Aged D018441:Biofilms D016022:Case-Control Studies D029754:Catha D000697:Central Nervous System Stimulants D003188:Composite Resins D003723:Dental Amalgam D003764:Dental Materials D017266:Dental Prosthesis D005260:Female D006801:Humans D008297:Male D008409:Mastication D064307:Microbiota D008875:Middle Aged D009909:Oral Health D012529:Saudi Arabia D013291:Streptococcus D055815:Young Adult
oral biofilm Khat restorative materials streptococcus veillonella prosthetic materials