Regulatory control of Sgs1 and Dna2 during eukaryotic DNA end resection.
In the repair of DNA double-strand breaks by homologous recombination, the DNA break ends must first be processed into 3' single-strand DNA overhangs. In budding yeast, end processing requires the helicase Sgs1 (BLM in humans), the nuclease/helicase Dna2, Top3-Rmi1, and replication protein A (RPA). Here, we use single-molecule imaging to visualize Sgs1-dependent end processing in real-time. We show that Sgs1 is recruited to DNA ends through Top3-Rmi1-dependent or -independent means, and in both cases Sgs1 is maintained in an immoble state at the DNA ends. Importantly, the addition of Dna2 triggers processive Sgs1 translocation, but DNA resection only occurs when RPA is also present. We also demonstrate that the Sgs1-Dna2-Top3-Rmi1-RPA ensemble can efficiently disrupt nucleosomes, and that Sgs1 itself possesses nucleosome remodeling activity. Together, these results shed light on the regulatory interplay among conserved protein factors that mediate the nucleolytic processing of DNA ends in preparation for homologous recombination-mediated chromosome damage repair.
['Xue|Chaoyou|C|', 'Wang|Weibin|W|', 'Crickard|J Brooks|JB|', 'Moevus|Corentin J|CJ|', 'Kwon|Youngho|Y|', 'Sung|Patrick|P|', 'Greene|Eric C|EC|']
D053903:DNA Breaks, Double-Stranded D004265:DNA Helicases D004260:DNA Repair D059765:Homologous Recombination D009707:Nucleosomes D053484:RecQ Helicases D051716:Replication Protein A D012441:Saccharomyces cerevisiae D029701:Saccharomyces cerevisiae Proteins D000072760:Single Molecule Imaging
DNA end resection homologous recombination helicase DNA repair single molecule