Molecular and Functional Phenotypes of Human Bone Marrow-Derived Mesenchymal Stromal Cells Depend on Harvesting Techniques.
Mesenchymal stromal cells (MSC) harvested in different tissues from the same donor exhibit different phenotypes. Each phenotype is not only characterized by a certain pattern of cell surface markers, but also different cellular functionalities. Only recently were different harvesting and processing techniques found to contribute to this phenomenon as well. This study was therefore set up to investigate proteomic and functional properties of human bone marrow-derived MSCs (hBM-MSC). These were taken from the same tissue and donor site but harvested either as aspirate or bone chip cultures. Both MSC populations were profiled for MSC markers defined by the International Society for Cellular Therapy (ISCT), MSC markers currently under discussion and markers of particular interest. While classic ISCT MSC markers did not show any significant difference between aspirate and outgrowth hBM-MSCs, our additional characterization panel revealed distinct patterns of differentially expressed markers. Furthermore, hBM-MSCs from aspirate cultures demonstrated a significantly higher osteogenic differentiation potential than outgrowth MSCs, which could be confirmed using a transcriptional approach. Our comparison of MSC phenotypes obtained by different harvesting techniques suggests the need of future standardized harvesting, processing and phenotyping procedures in order to gain better comparability in the MSC field.
['Walter|Sebastian G|SG|', 'Randau|Thomas M|TM|0000-0002-5224-9639', 'Hilgers|Cäcilia|C|', 'Haddouti|El-Mustapha|EM|', 'Masson|Werner|W|', 'Gravius|Sascha|S|', 'Burger|Christof|C|', 'Wirtz|Dieter C|DC|', 'Schildberg|Frank A|FA|0000-0003-0797-1945']
D050156:Adipogenesis D015415:Biomarkers D001707:Biopsy, Needle D018929:Cell Culture Techniques D002454:Cell Differentiation D049109:Cell Proliferation D002478:Cells, Cultured D020219:Chondrogenesis D006801:Humans D059630:Mesenchymal Stem Cells D010012:Osteogenesis D010641:Phenotype D040901:Proteomics D013048:Specimen Handling
phenotype mesenchymal stromal cells harvesting technique characterization differentiation osteoimmunology