Structural features of an acidic polysaccharide with the potential of promoting osteoblast differentiation from Lycium ruthenicum Murr.
The enhanced osteoblast differentiation is beneficial to the prevention of osteoporosis. In this study, a homogeneous polysaccharide (LRP-S2A) with the potential of promoting osteoblast differentiation was obtained from the fruits of Lycium ruthenicum, a traditional herb for treatment of postmenopausal metabolic disorders. Structural identification indicated that LRP-S2A, with a relative molecular weight of 2.65 × 106 Da and an uronic acid content of 41.8%, contained Rha, Ara, Gal, Glc and GlcA in a molar ratio of 1.00 : 2.07 : 0.57 : 2.59 : 4.33 and was composed of a backbone consisting of 6-O-Me-α-(1→4)-D-GlcpA, 2-O-acetyl-α-(1→4)-D-Glcp, α-(1→2,4)-L-Rhap, β-(1→3)-D-Galp andα-(1→3,5)-L-Araf, and some branches consisting of 6-O-Me-α-(1→4)-D-GlcpA and terminal α-L-Araf. These results suggested that LRP-S2A with the potential of promoting osteoblast differentiation was a new acidic polysaccharide.
['Wang|Shu-Qi|SQ|', 'Liu|Bing|B|', 'Liu|Su|S|', 'Xie|Song-Zi|SZ|', 'Pan|Li-Hua|LH|', 'Zha|Xue-Qiang|XQ|', 'Li|Qiang-Ming|QM|', 'Luo|Jian-Ping|JP|']
D000818:Animals D002454:Cell Differentiation D002478:Cells, Cultured D005638:Fruit D006801:Humans D032305:Lycium D008970:Molecular Weight D010006:Osteoblasts D011134:Polysaccharides D014574:Uronic Acids
acidic polysaccharide osteoblast differentiation Lycium ruthenicum Murr. structure elucidation