Toward immunosuppressive effects on liver transplantation in rat model: tacrolimus loaded poly(ethylene glycol)-poly(D,L-lactide) nanoparticle with longer survival time.
In this study, tacrolimus (FK506) was encapsulated into a biodegradable poly(ethylene glycol)-poly(D,L-lactide) (MPEG-PLA) block copolymer using a double emulsion-solvent evaporation technique. Drug loading (DL) and encapsulation efficiency (EE) can be changed by varying the mass ratio of FK506/MPEG-PLA. Furthermore, transmission electron microscope (TEM) and Malvern Zetasizer were used to investigate the properties of FK506/MPEG-PLA nanoparticles (DL=9.5%), which were monodisperse (PDI=0.100 ± 0.023) with a mean particle size of 90.5 ± 1.5 nm. Compared with FK506 capsule, in vitro release profile showed that FK506/MPEG-PLA nanoparticles exhibited sustained release. Meanwhile, the higher concentration and longer retention time in plasma were also confirmed in vivo. We further preliminarily evaluated immunosuppressive effect on liver transplantation in rat model. The survival time of the rat administrated FK506/MPEG-PLA nanoparticles was obviously prolonged than that of the control group administrated FK506 capsule.
['Xu|Wei|W|', 'Ling|Peixue|P|', 'Zhang|Tianmin|T|']
D000818:Animals D002470:Cell Survival D004337:Drug Carriers D057809:HEK293 Cells D006801:Humans D007166:Immunosuppressive Agents D016031:Liver Transplantation D008297:Male D046529:Microscopy, Electron, Transmission D053758:Nanoparticles D010316:Particle Size D011091:Polyesters D011092:Polyethylene Glycols D051381:Rats D017207:Rats, Sprague-Dawley D017208:Rats, Wistar D016559:Tacrolimus
Immunosuppressive Liver transplantation Tacrolimus Nanoparticle MPEG-PLA