LncRNA SNHG1 regulates vascular endothelial cell proliferation and angiogenesis via miR-196a.
Inflammatory cytokines are important protagonists in the formation of atherosclerotic plaques, triggering effects throughout the atherosclerotic vessels due to the destruction in proliferation, migration and angiogenesis of endothelial cells. In this study, we found SNHG1 is upregulated in TNF-α-treated HUVECs. We silenced SNHG1 and found it inhibited vascular endothelial cell proliferation and angiogenesis. In the other hand, exogenetic overexpression of SNHG1 promotes proliferation, migration and angiogenesis. Then we demonstrated that SNHG1 may interact directly with miR-196a to act as a miR-196a sponge. Further, MAPK6 were predicted to be the target of miR-196a. So we blocked miR-196a, which increased expression level of MAPK6, enhanced cell proliferation, migration and angiogenesis. These data indicated that SNHG1/miR-196a/MAPK6 axis may take a part in autophagy regulation in TNF-α-treated HUVECs. The subsequent rescue experiments come to the results ascertained the specificity of SNHG1/miR-196a/MAPK6 axis in regulating MAPK6. Overall, our findings demonstrate a novel mechanism by which SNHG1 overexpression protects the function of HUVECs, which may delay the progression of AS. SNHG1/miR-196a/MAPK6 axis may be of therapeutic significance in AS.
['Zhang|Liping|L|', 'Zhang|Qiang|Q|', 'Lv|Lingxia|L|', 'Jianhua|Zhu|Z|', 'Ting|Chen|C|', 'Wu|Yutao|Y|']
D049109:Cell Proliferation D042783:Endothelial Cells D005786:Gene Expression Regulation D061307:Human Umbilical Vein Endothelial Cells D006801:Humans D035683:MicroRNAs D048053:Mitogen-Activated Protein Kinase 6 D009389:Neovascularization, Pathologic D034622:RNA Interference D062085:RNA, Long Noncoding D014409:Tumor Necrosis Factor-alpha
SNHG1 Inflammatory cytokines HUVECs Atherosclerosis