Synthesis of copper-containing bioactive glass nanoparticles using a modified Stöber method for biomedical applications.
Copper (Cu)-containing bioactive glasses (BGs) are attracting attention for bone regeneration and wound healing since they have bone-bonding capability and potential osteogenesis and angiogenesis properties. In this study, highly dispersed and spherical Cu-containing bioactive glass nanoparticles (Cu-BGNs) were successfully synthesized via a modified Stöber method. The content of incorporated Cu in the particles could be tailored by adjusting the amount of the added Cu precursor, a procedure that had no significant effects on the morphological and structural characteristics of the nanoparticles. Cu-BGNs exhibited satisfactory apatite-forming ability, as a large quantity of apatite could form on Cu-BGNs pellets after immersion in simulated body fluid for just 3days. The incorporation of Cu exhibited positive effects on the apatite formation. In addition, both Si and Cu ions were released from the Cu-BGN in a sustained manner for at least 14days in cell culture medium, indicating the potential of the BGN as promising carriers for delivering therapeutic Cu ions. Moreover, Cu-BGNs showed no significant cytotoxicity towards human mesenchymal stem cells and fibroblast cells at concentrations of 100, 10 and 1μg/mL. Taken together, the results suggest that Cu-BGNs are promising nanoparticulate fillers to develop nanocomposites for biomedical applications especially in bone regeneration and wound healing.
['Zheng|Kai|K|', 'Dai|Xinyi|X|', 'Lu|Miao|M|', 'Hüser|Norbert|N|', 'Taccardi|Nicola|N|', 'Boccaccini|Aldo R|AR|']
D001031:Apatites D001672:Biocompatible Materials D001861:Bone Regeneration D018929:Cell Culture Techniques D003300:Copper D005347:Fibroblasts D005898:Glass D006801:Humans D007477:Ions D059630:Mesenchymal Stem Cells D053761:Nanocomposites D053758:Nanoparticles D036103:Nanotechnology D009389:Neovascularization, Pathologic D010012:Osteogenesis D010316:Particle Size D017550:Spectroscopy, Fourier Transform Infrared D014945:Wound Healing D014961:X-Ray Diffraction
Bioactive glass nanoparticles Stöber method Copper Apatite formation Ion release