Basal and insulin-regulated VLDL1 and VLDL2 kinetics in men with type 2 diabetes.
AIMS/HYPOTHESIS
Hypertriacylglycerolaemia is a hallmark of diabetic dyslipidaemia with increased concentrations of triacylglycerol (TG)-rich VLDL1 particles. However, whether VLDL1 secretion or removal is abnormal in type 2 diabetes remains unclear. The aim of this study was to compare basal and insulin-mediated VLDL1- and VLDL2-TG kinetics in men with type 2 diabetes and healthy men using a novel direct VLDL1- and VLDL2-TG labelling method.


METHODS
Twelve men with type 2 diabetes and 12 healthy men matched for age and BMI were recruited. VLDL1- and VLDL2-TG turnover were measured during a 4 h basal period and a 3.5 h hyperinsulinaemic clamp period using a primed-constant infusion of ex vivo labelled VLDL1-TG and VLDL2-TG.


RESULTS
Basal VLDL1-TG and VLDL2-TG secretion rates were similar in men with diabetes and healthy men. During hyperinsulinaemia, VLDL1-TG secretion rates were suppressed significantly in both groups, whereas no suppression of VLDL2-TG secretion rate was observed. VLDL1-TG to VLDL2-TG transfer rate was not significantly different from zero in both groups, while VLDL1-TG fatty acid oxidation rate was substantial, with a contribution to total energy expenditure of approximately 15% during postabsorptive conditions. VLDL1 and VLDL2 particle size (TG/apolipoprotein B [apoB] ratio) and apoB-100 concentration were unaltered by hyperinsulinaemia in men with type 2 diabetes, but significantly reduced in healthy men.


CONCLUSIONS/INTERPRETATION
Insulin inhibits VLDL1-TG secretion rate similarly in age- and BMI-matched men with type 2 diabetes and healthy men, while VLDL2-TG secretion is unaltered by hyperinsulinaemia. However, VLDL1- and VLDL2-apoB levels are not lowered by hyperinsulinaemia in men with type 2 diabetes, which is indicative of a diminished hepatic response to insulin.


TRIAL REGISTRATION
ClinicalTrials.gov NCT01564550.
['Johansen|Rakel F|RF|', 'Søndergaard|Esben|E|', 'Sørensen|Lars Peter|LP|', 'Jurik|Anne Grethe|AG|', 'Christiansen|Jens S|JS|', 'Nielsen|Søren|S|']
D000328:Adult D000368:Aged D016022:Case-Control Studies D003924:Diabetes Mellitus, Type 2 D006801:Humans D007328:Insulin D007333:Insulin Resistance D007700:Kinetics D050356:Lipid Metabolism D008079:Lipoproteins, VLDL D008297:Male D008875:Middle Aged
VLDL1 VLDL2 Kinetics Type 2 diabetes Dyslipidaemia Healthy men Hyperinsulinaemic clamp Insulin resistance