Immunoblotting using Strongyloides venezuelensis larvae, parthenogenetic females or eggs extracts for the diagnosis of experimentally infected immunosuppressed rats.
The nematode Strongyloides stercoralis is responsible for strongyloidiasis in humans. Diagnosis of infection occurs through detection of larvae in feces, but low elimination of larvae often hampers the detection of disease, particularly in cases of patient immunosuppression. Immunodiagnostic tests have been developed; however obtaining S. stercoralis larvae for the production of homologous antigen extract is technically difficult. Thus, the use different developmental forms of Strongyloides venezuelensis has become an alternative method for the production of antigen extracts. The aim of this study was to evaluate immunoblotting using alkaline extracts from S. venezuelensis L3 larvae, parthenogenetic females or eggs to test detection of experimental strongyloidiasis associated with immunosuppression. Immunocompetent and immunosuppressed male rats were experimentally infected, and serum sample from all animals were obtained at 0, 5, 8 13, and 21 days post infection (d.p.i.). Immunoblotting was evaluated for use in detection of anti-S. venezuelensis IgG in both experimental rat groups. The larval extract immunoblotting profile had the most immunoreactive fractions in the immunosuppressed group beginning at 5 d.p.i., while the immunocompetent group reactivity began on 8 d.p.i. Immunoreactive protein fractions of 17 kDa present in larval alkaline extract presented as possible markers of infection in immunosuppressed rats. It is concluded that all extracts using immunoblotting have diagnostic potential in experimental strongyloidiasis, particularly larval extract in immunosuppressed individuals.
['Goulart de Carvalho|Edson Fernando|EF|', 'Neto de Sousa|José Eduardo|JE|', 'Gonçalves|Ana Lúcia Ribeiro|AL|', 'da Cunha-Junior|Jair Pereira|JP|', 'Costa-Cruz|Julia Maria|JM|']
D000818:Animals D000909:Antibodies, Helminth D000947:Antigens, Helminth D004195:Disease Models, Animal D004591:Electrophoresis, Polyacrylamide Gel D005243:Feces D005260:Female D015151:Immunoblotting D007121:Immunocompetence D016867:Immunocompromised Host D007074:Immunoglobulin G D007814:Larva D008297:Male D010063:Ovum D010312:Parthenogenesis D051381:Rats D017208:Rats, Wistar D013321:Strongyloides D013322:Strongyloidiasis
Immunoblotting Strongyloides venezuelensis Rats Immunossupression