Simultaneous determination of leucine, isoleucine and valine in Beagle dog plasma by HPLC-MS/MS and its application to a pharmacokinetic study.
Leucine (Leu), isoleucine (Ile) and valine (Val) are three branched-chain amino acids (BCAAs), which have been widely used as dietary supplements for professional athletes and patients with liver failure or catabolic diseases. To date, no pharmacokinetic studies of BCAAs in vivo useful for the assessment of clinical effect following daily intake has been reported. Thus in this study, an HPLC-MS/MS method for simultaneous determination of Leu, Ile and Val in Beagle dog plasma using homoarginine as the internal standard was developed and validated in terms of specificity, linearity, precision, accuracy, and stability. This assay method was then applied to a pharmacokinetic study of BCAAs in dogs following oral administration of 0.25 g/kg and 0.50 g/kg BCAAs. The HPLC-MS/MS method was found to be sensitive and reproducible for quantification of BCAAs in dog plasma and successfully applied to the pharmacokinetic study. All these BCAAs were well absorbed with a substantial increase in the plasma concentration after a baseline modification. No statistical significance was identified in different gender group and no drug accumulation was observed following multiple doses.
['Wang|Ting|T|', 'Xie|Huiru|H|', 'Chen|Xu|X|', 'Jiang|Xuehua|X|', 'Wang|Ling|L|']
D000284:Administration, Oral D000818:Animals D002138:Calibration D002851:Chromatography, High Pressure Liquid D004285:Dogs D005260:Female D007532:Isoleucine D007930:Leucine D008297:Male D010949:Plasma D011786:Quality Control D015203:Reproducibility of Results D053719:Tandem Mass Spectrometry D014633:Valine
Beagle dog plasma Pharmacokinetics BCAAs Gender HPLC–MS/MS