Detection and quantification of cytosolic DNA.
Cytosolic DNA sensing is emerging to be a critical component of the antitumor immune response by jumpstarting innate immune responses subsequent to the stimulation of the cGAS and STING pathway. Investigating the accumulation of DNA species in the cytosol is therefore an essential readout for promising anticancer strategies. In this chapter, we present different techniques that can be utilized to detect and quantify cytosolic DNA accumulation.
['Spada|Sheila|S|', 'Yamazaki|Takahiro|T|', 'Vanpouille-Box|Claire|C|']
D018929:Cell Culture Techniques D045744:Cell Line, Tumor D003600:Cytosol D004247:DNA D019085:Fluorescent Antibody Technique, Direct D006801:Humans D007113:Immunity, Innate D008565:Membrane Proteins D009369:Neoplasms D009713:Nucleotidyltransferases D015398:Signal Transduction D014461:Ultracentrifugation
Cytosolic DNA DNA sensing Innate immunity cGAS STING Interferon type I