Effective Delivery of Arsenic Trioxide to HPV-Positive Cervical Cancer Cells Using Optimised Liposomes: A Size and Charge Study.
Despite the success of arsenic trioxide (ATO) in treating haematological malignancies, its potential to treat solid tumours has not been fully exploited, owing to its dose-limiting toxicity and poor pharmacokinetics. In order to overcome this hurdle, liposomal encapsulation of the drug with different surface charges (neutral, negative, and positive) and sizes (100, 200 and 400 nm) were synthesised and tested on human papilloma virus (HPV)-positive HeLa and HPV-negative HT-3 cervical cancer cell lines. Two epithelial cell lines-human keratinocytes (HK) and human colon cells (CRL-1790)-were used as controls. The synthesised liposomes were tested for their physico-chemical characteristics, drug loading efficiency, and toxicity on the studied cell lines. Neutral liposomes of 100 nm in size were the chosen formulation for delivering ATO into the studied cells, as they showed the least intrinsic cytotoxicity and the highest loading efficiency. The findings demonstrated that the optimised formulation of liposomes was an effective drug delivery method for HPV-infected cervical cancer cells. Furthermore, the toxicity vs. uptake ratio was highest for HeLa cells, while a reduced or minimal toxic effect was observed for non-HPV-infected cervical cancer cells and control cells. These findings may provide a promising therapeutic strategy for effectively managing cervical cancers.
['Akhtar|Anam|A|', 'Wang|Scarlet Xiaoyan|SX|', 'Ghali|Lucy|L|', 'Bell|Celia|C|', 'Wen|Xuesong|X|']
D000970:Antineoplastic Agents D000077237:Arsenic Trioxide D001152:Arsenicals D045744:Cell Line, Tumor D002470:Cell Survival D004339:Drug Compounding D016503:Drug Delivery Systems D005260:Female D006801:Humans D008081:Liposomes D010087:Oxides D030361:Papillomavirus Infections D010316:Particle Size D055672:Static Electricity D002583:Uterine Cervical Neoplasms
cervical cancer liposome arsenic trioxide (ATO) drug delivery human papilloma virus (HPV)