Functionalized PCL/HA nanocomposites as microporous membranes for bone regeneration.
In the present work, microporous membranes based on poly(ε-caprolactone) (PCL) and PCL functionalized with amine (PCL-DMAEA) or anhydride groups (PCL-MAGMA) were realized by solvent-non solvent phase inversion and proposed for use in Guided Tissue Regeneration (GTR). Nanowhiskers of hydroxyapatite (HA) were also incorporated in the polymer matrix to realize nanocomposite membranes. Scanning Electron Microscopy (SEM) showed improved interfacial adhesion with HA for functionalized polymers, and highlighted substantial differences in the porosity. A relationship between the developed porous structure of the membrane and the chemical nature of grafted groups was proposed. Compared to virgin PCL, hydrophilicity increases for functionalized PCL, while the addition of HA influences significantly the hydrophilic characteristics only in the case of virgin polymer. A significant increase of in vitro degradation rate was found for PCL-MAGMA based membranes, and at lower extent of PCL-DMAEA membranes. The novel materials were investigated regarding their potential as support for cell growth in bone repair using multipotent mesenchymal stromal cells (MSC) as a model. MSC plated onto the various membranes were analyzed in terms of adhesion, proliferation and osteogenic capacity that resulted to be related to chemical as well as porous structure. In particular, PCL-DMAEA and the relative nanocomposite membranes are the most promising in terms of cell-biomaterial interactions.
['Basile|Maria Assunta|MA|', "d'Ayala|Giovanna Gomez|GG|", 'Malinconico|Mario|M|', 'Laurienzo|Paola|P|', 'Coudane|Jean|J|', 'Nottelet|Benjamin|B|', 'Ragione|Fulvio Della|FD|', 'Oliva|Adriana|A|']
D001861:Bone Regeneration D002448:Cell Adhesion D002454:Cell Differentiation D049109:Cell Proliferation D002478:Cells, Cultured D017886:Durapatite D005260:Female D006801:Humans D008297:Male D008567:Membranes, Artificial D059630:Mesenchymal Stem Cells D053761:Nanocomposites D010012:Osteogenesis D011091:Polyesters D016062:Porosity
Nanocomposites Membranes Poly(ε-caprolactone) Guided tissue regeneration Biocompatibility Functionalization of polymers