Identification of the Minimal Glycotope of Streptococcus pneumoniae 7F Capsular Polysaccharide using Synthetic Oligosaccharides.
Streptococcus pneumoniae causes life-threatening diseases including meningitis, pneumonia and sepsis. Existing glycoconjugate vaccines based on purified capsular polysaccharides are widely used and help to prevent millions of deaths every year. Herein, the total syntheses of oligosaccharides resembling portions of the S. pneumoniae serotype 7F (ST7F) capsular polysaccharide repeating unit are reported. To define minimal glycan epitopes, glycan microarrays containing the synthetic oligosaccharides were used to screen human reference serum and revealed that both side chains of the ST7F play a key role in antigen recognition. The identification of protective minimal epitopes is vital to design efficient semi- and fully-synthetic glycoconjugate vaccines.
['Ménová|Petra|P|http://orcid.org/0000-0002-8450-194X', 'Sella|Mauro|M|', 'Sellrie|Katrin|K|', 'Pereira|Claney L|CL|http://orcid.org/0000-0003-1972-7907', 'Seeberger|Peter H|PH|http://orcid.org/0000-0003-3394-8466']
D000939:Epitopes D006001:Glycoconjugates D006801:Humans D022242:Pneumococcal Vaccines D011134:Polysaccharides D011135:Polysaccharides, Bacterial D065288:Serogroup D013296:Streptococcus pneumoniae
minimal glycotopes Streptococcus pneumoniae serotype 7F glycan arrays total synthesis