Long non-coding RNA TUG1 promotes cell proliferation and metastasis by negatively regulating miR-300 in gallbladder carcinoma.
BACKGROUND
As we all know, long non-coding RNAs (lncRNAs) have been reported to play vital roles in various human cancers. In this study, we aimed to explore the role of lncRNA TUG1 in gallbladder carcinoma (GBC) development.


METHODS
Total RNA was extracted from the tissues of thirty GBC patients, four GBC cell lines. We detected the expression levels of TUG1 using quantitative real-time PCR. We performed CCK8, colony formation, transwell invasion and apoptosis assays to study the effects of TUG1 on GBC cell proliferation and invasion. Western blot assay was performed to assess to the expression level of epithelial-mesenchymal transition (EMT) markers in transforming growth factor-β1 (TGF-β1) treated and TUG1 knockdown GBC cell. Lastly, dual-luciferase reporter assay and quantitative real-time PCR were performed to verify the potential target microRNAs (miRNAs) of TUG1.


RESULTS
TUG1 expression was significantly overexpressed in GBC tissues. Functionally, this study demonstrated that knockdown of TUG1 significantly inhibited GBC cell proliferation, metastasis. Mechanically, we found that TUG1 is upregulated by TGF-β1, and knockdown of TUG1 inhibited GBC cell EMT. Furthermore, we identified that miR-300, which has been reported as a suppressor in other types of cancer, is negatively regulated by TUG1.


CONCLUSIONS
LncRNA TUG1 promotes GBC cell proliferation, metastasis and EMT progression by functioning as a miRNA sponge to abrogate the endogenous effect of miR-300.
['Ma|Fei|F|', 'Wang|Shou-Hua|SH|', 'Cai|Qiang|Q|', 'Jin|Long-Yang|LY|', 'Zhou|Di|D|', 'Ding|Jun|J|', 'Quan|Zhi-Wei|ZW|']
D001483:Base Sequence D045744:Cell Line, Tumor D049109:Cell Proliferation D058750:Epithelial-Mesenchymal Transition D005706:Gallbladder Neoplasms D015972:Gene Expression Regulation, Neoplastic D055785:Gene Knockdown Techniques D006801:Humans D035683:MicroRNAs D009362:Neoplasm Metastasis D062085:RNA, Long Noncoding
Long non-coding RNA TUG1 Proliferation Metastasis miR-300 Gallbladder carcinoma