Stress oxidative and genotoxicity in Prochilodus lineatus (Valenciennes, 1836) exposed to commercial formulation of insecticide cypermethrin.
The use of toxic pesticides has become a world problem because they can contaminate streams and rivers, producing an adverse impact on non-target aquatic biota, including fishes. Cypermethrin is one of the most important insecticides to control ectoparasites in wide-scale. The aim of this study was to evaluate the effect of commercial formulations of cypermethrin, SHERPA O (0.0, 0.075, 0.15, and 0.3 µg/L of cypermethrin) in fish Prochilodus lineatus for 96 h in semi-static condition, using biomarkers of genotoxicity: micronucleus frequency (MNF) in erythrocytes and biomarkers of oxidative damage: lipid peroxidation (TBARS) and antioxidant defenses, catalase (CAT) and glutathione (GSH) in liver tissue. Our results showed a significant decrease (p < 0.05) of CAT at pesticide concentrations of 0.150 and 0.300 μg/L, but no significant difference was observed in TBARS or GSH in any exposed group (p > 0.05) in comparison to the control. A significant increase was observed in the MNF in the group exposed to 0.3 μg/L of cypermethrin compared to negative control (p < 0.05). Finally, P. lineatus proved to be a sensitive species to the commercial formulations of cypermethrin and that CAT and MNF are effective indicators of these toxic effects.
['Davico|C E|CE|', 'Loteste|A|A|', 'Parma|M J|MJ|', 'Poletta|G|G|', 'Simoniello|M F|MF|']
D000818:Animals D000975:Antioxidants D002374:Catalase D060125:Characiformes D004305:Dose-Response Relationship, Drug D004912:Erythrocytes D005978:Glutathione D007306:Insecticides D015227:Lipid Peroxidation D015162:Micronucleus Tests D018384:Oxidative Stress D011722:Pyrethrins
Fish biomarkers micronucleus frequency catalase liver tissue