Transport Characteristics of 6-Mercaptopurine in Brain Microvascular Endothelial Cells Derived From Human Induced Pluripotent Stem Cells.
The likelihood of reoccurrence of acute lymphoblastic leukemia is influenced by the cerebral concentration of the therapeutic agent 6-mercaptopurine (6-MP) during treatment. Therefore, it is important to understand the blood-brain barrier (BBB) transport mechanism of 6-MP. The purpose of this study was to characterize this mechanism using human induced pluripotent stem cell-derived microvascular endothelial cells (hiPS-BMECs). The permeability coefficient of 6-MP across hiPS-BMECs monolayer in the basal-to-apical direction (B-to-A) was significantly greater than that in the opposite direction (A-to-B). The inhibition profiles of 6-MP transport in the A-to-B direction were different from those in the B-to-A direction. Transport in the A-to-B direction was mainly inhibited by adenine (an inhibitor of equilibrative nucleobase transporter 1; ENBT1), while transport in the B-to-A direction was significantly reduced by inhibitors of multidrug resistance-associated proteins (MRPs), especially zaprinast (an MRP5 inhibitor). Immunocytochemical analyses demonstrated the expression of ENBT1 and MRP5 proteins in hiPS-BMECs. We confirmed that the cellular uptake of 6-MP is decreased by ENBT1 inhibitors in hiPS-BMECs and by knockdown of ENBT1 in hCMEC/D3 cells. These results suggest that ENBT1 and MRP5 make substantial contributions to the transport of 6-MP in hiPS-BMECs and hCMEC/D3 cells.
['Kurosawa|Toshiki|T|', 'Tega|Yuma|Y|', 'Sako|Daiki|D|', 'Mochizuki|Tatsuki|T|', 'Yamaguchi|Tomoko|T|', 'Kawabata|Kenji|K|', 'Inoue|Katsuhisa|K|', 'Ito|Naoki|N|', 'Kusuhara|Hiroyuki|H|', 'Deguchi|Yoshiharu|Y|']
D001692:Biological Transport D001812:Blood-Brain Barrier D001921:Brain D042783:Endothelial Cells D006801:Humans D057026:Induced Pluripotent Stem Cells D015122:Mercaptopurine
Human induced pluripotent stem cells Blood-brain barrier hCMEC/D3 cells ABC transporter Membrane transport SLC transporter