Salvianolate lyophilized injection (SLI) strengthens blood-brain barrier function related to ERK1/2 and Akt signaling pathways.
The salvianolate lyophilized injection (SLI) has been widely used for the treatment of acute cerebral infarction; however, the molecular mechanism of how it strengthens blood brain barrier (BBB) function is not well understood. Here, we investigated the effects of SLI on BBB function in bEnd.3 cells as well as in rats. In oxygen glucose deprivation/reoxygenation (OGD/R)-damaged bEnd.3 cells, SLI increased transepithelial electrical resistance and decreased sodium fluorescein flux. SLI-treated cells showed increased expression of tight junction proteins, including Zonula occludens-1 (ZO-1), Claudin-5 and Occludin. Furthermore, SLI led to the decrease of phosphorylation of ERK1/2, p38, and Akt. Using selective inhibitors, we found that the positive effects of SLI on barrier function were abolished in cells in which ERK1/2 and Ak signaling were inhibited. Moreover, in MCAO model rats, SLI effectively alleviated brain leakage of Evans blue, increased brain tissue ZO-1 expression and inhibited phosphorylation of ERK1/2 and Akt. Overall, these data suggest that SLI strengthens BBB function was interrelated ERK1/2 and Akt signaling pathways in cerebral vascular diseases.
['Zhao|Chun|C|', 'Li|Xueli|X|', 'Li|Xiaojin|X|', 'Xu|Yangyang|Y|', 'Ma|Mengmeng|M|', 'Wang|Shaoxia|S|', 'Chai|Lijuan|L|', 'Guo|Hong|H|', 'Hu|Limin|L|']
D000818:Animals D001812:Blood-Brain Barrier D001921:Brain D002545:Brain Ischemia D002460:Cell Line D062507:Claudin-5 D020935:MAP Kinase Signaling System D008297:Male D051379:Mice D018696:Neuroprotective Agents D062793:Occludin D010936:Plant Extracts D051057:Proto-Oncogene Proteins c-akt D051381:Rats D017208:Rats, Wistar D015398:Signal Transduction D062725:Tight Junction Proteins D019108:Tight Junctions D062826:Zonula Occludens-1 Protein
Salvianolate lyophilized injection ERK1/2 Akt Blood brain barrier Tight junction