Loss of miR-83 extends lifespan and affects target gene expression in an age-dependent manner in Caenorhabditis elegans.
MicroRNAs (miRNAs) are short non-coding RNAs that are involved in the post-transcriptional regulation of protein-coding genes. miRNAs modulate lifespan and the aging process in a variety of organisms. In this study, we identified a role of miR-83 in regulating lifespan of Caenorhabditis elegans. mir-83 mutants exhibited extended lifespan, and the overexpression of miR-83 was sufficient to decrease the prolonged lifespan of the mutants. We observed upregulation of the expression levels of a set of miR-83 target genes in young mir-83 mutant adults; while different sets of genes were upregulated in older mir-83 mutant adults. In vivo assays showed that miR-83 regulated expression of target genes including din-1, spp-9 and col-178, and we demonstrated that daf-16 and din-1 were required for the extension of lifespan in the mir-83 mutants. The regulation of din-1 by miR-83 during aging resulted in the differential expression of din-1 targets such as gst-4 and gst-10. In daf-2 mutants, the expression level of miR-83 was significantly reduced compared to wild-type animals. We identified a role for miR-83 in modulating lifespan in C. elegans and provided molecular insights into its functional mechanism.
['Dzakah|Emmanuel Enoch|EE|', 'Waqas|Ahmed|A|', 'Wei|Shuai|S|', 'Yu|Bin|B|', 'Wang|Xiaolin|X|', 'Fu|Tao|T|', 'Liu|Lei|L|', 'Shan|Ge|G|']
D000818:Animals D017173:Caenorhabditis elegans D029742:Caenorhabditis elegans Proteins D005260:Female D051858:Forkhead Transcription Factors D018507:Gene Expression Regulation, Developmental D008136:Longevity D008297:Male D035683:MicroRNAs D014157:Transcription Factors
miR-83 din-1 daf-2 C. elegans Longevity