Novel spectrophotometric determination of flumethasone pivalate and clioquinol in their binary mixture and pharmaceutical formulation.
This work is concerned with development and validation of three simple, specific, accurate and precise spectrophotometric methods for determination of flumethasone pivalate (FP) and clioquinol (CL) in their binary mixture and ear drops. Method A is a ratio subtraction spectrophotometric one (RSM). Method B is a ratio difference spectrophotometric one (RDSM), while method C is a mean center spectrophotometric one (MCR). The calibration curves are linear over the concentration range of 3-45 μg/mL for FP, and 2-25 μg/mL for CL. The specificity of the developed methods was assessed by analyzing different laboratory prepared mixtures of the FP and CL. The three methods were validated as per ICH guidelines; accuracy, precision and repeatability are found to be within the acceptable limits.
['Abdel-Aleem|Eglal A|EA|', 'Hegazy|Maha A|MA|', 'Sayed|Nour W|NW|', 'Abdelkawy|M|M|', 'Abdelfatah|Rehab M|RM|']
D000890:Anti-Infective Agents D007464:Clioquinol D004338:Drug Combinations D005443:Flumethasone D005938:Glucocorticoids D019999:Pharmaceutical Solutions D015203:Reproducibility of Results D013053:Spectrophotometry
Flumethasone Clioquinol Binary mixture Ratio subtraction Ratio difference Mean centering