Temporal variation in the deposition of different types of collagen within a porous biomaterial implant.
The deposition of new collagen in association with a medical implant has been studied using expanded polytetrafluoroethylene vascular replacement samples implanted subcutaneously in sheep, for up to 28 days. New type I collagen mRNA synthesis was followed by in situ hybridization, while the accumulation of new collagen types III, V, VI, XII, and XIV was followed by immunohistochemistry. All the collagen detected in the pores of the implant were newly deposited at various times after implantation and were not due to any pre-existing dermal collagen that may have been present around the implant. Collagen deposition was seen initially surrounding the implant and, with time, was seen to infiltrate within its pores. In situ hybridization showed that the majority of infiltrating cells had switched on mRNA that coded for type I collagen production. Histology showed that cellular infiltration increased with time, accompanied by increasing collagen deposition. The deposition of different collagen types happened at different rates. The type V and VI collagens preceded the major interstitial collagens in the newly deposited tissue, although at longer time points, detection of type V collagen appeared to decrease. After disruption of the interstitial collagens with enzyme, the "masked" type V collagen was clearly still visible by immunohistochemistry. Little type XII collagen could be seen within the porous mesh, although it was seen in the surrounding tissues. By contrast, type XIV was seen throughout the porous structure of the implanted mesh, with less being visible outside the material where type XII was more abundant.
['White|Jacinta F|JF|', 'Werkmeister|Jerome A|JA|', 'Bisucci|Teresa|T|', 'Darby|Ian A|IA|', 'Ramshaw|John A M|JA|']
D000818:Animals D001672:Biocompatible Materials D003094:Collagen D007150:Immunohistochemistry D020007:Implants, Experimental D017403:In Situ Hybridization D011138:Polytetrafluoroethylene D016062:Porosity D012756:Sheep D013194:Staining and Labeling D013997:Time Factors
collagen in situ hybridization immunohistochemistry cell material interaction porous material