Characterization of different functionalized lipidic nanocapsules as potential drug carriers.
Lipid nanocapsules (LNC) based on a core-shell structure consisting of an oil-filled core with a surrounding polymer layer are known to be promising vehicles for the delivery of hydrophobic drugs in the new therapeutic strategies in anti-cancer treatments. The present work has been designed as basic research about different LNC systems. We have synthesized-and physico-chemically characterized-three different LNC systems in which the core was constituted by olive oil and the shell by different phospholipids (phosphatidyl-serine or lecithin) and other biocompatible molecules such as Pluronic(®) F68 or chitosan. It is notable that the olive-oil-phosphatidyl-serine LCN is a novel formulation presented in this work and was designed to generate an enriched carboxylic surface. This carboxylic layer is meant to link specific antibodies, which could facilitate the specific nanocapsule uptake by cancer cells. This is why nanoparticles with phosphatidyl-serine in their shell have also been used in this work to form immuno-nanocapsules containing a polyclonal IgG against a model antigen (C-reactive protein) covalently bounded by means of a simple and reproducible carbodiimide method. An immunological study was made to verify that these IgG-LNC complexes showed the expected specific immune response. Finally, a preliminary in vitro study was performed by culturing a breast-carcinoma cell line (MCF-7) with Nile-Red-loaded LNC. We found that these cancer cells take up the fluorescent Nile- Red molecule in a process dependent on the surface properties of the nanocarriers.
['Sánchez-Moreno|Paola|P|', 'Ortega-Vinuesa|Juan Luis|JL|', 'Martín-Rodríguez|Antonio|A|', 'Boulaiz|Houría|H|', 'Marchal-Corrales|Juan Antonio|JA|', 'Peula-García|José Manuel|JM|']
D002097:C-Reactive Protein D002626:Chemistry, Pharmaceutical D004337:Drug Carriers D016503:Drug Delivery Systems D004355:Drug Stability D006801:Humans D018796:Immunoconjugates D007074:Immunoglobulin G D008055:Lipids D061986:MCF-7 Cells D053769:Nanocapsules D010078:Oxazines D013499:Surface Properties
lipid nanocapsules immuno-nanocapsules nanocarriers drug delivery