Heat shock protein 70 as a supplementary receptor facilitates enterovirus 71 infections in vitro.
As one of the major causative agents of hand, foot and mouth disease (HFMD), enterovirus 71 (EV71) is a small, non-enveloped positive stranded RNA virus. Children suffering EV71 infection may cause severe symptoms including neurological complications, pulmonary edema and aseptic meningitis. EV71 is a neurotropic virus and it can cause the damage of nervous cells, cytokine storm and toxic substance. Identifying the factors that mediate viral binding or entry to host cells is important to uncover the mechanisms which viruses utilize to cause diseases in human body. Heat shock protein 70 (HSP70) is induced during virus infection and facilitates proper protein folding during viral propagation. The role that HSP70 plays during EV71 infection is still unclear. In this study, siRNA interference technique and transgenic technique were used to investigate the interaction between HSP70 and EV71 virus. The result demonstrated that the cell surface HSP70 is not essential for EV71 infection but helps the initial binding of virus to host cells and that multiple receptors are involved during EV71 infection. In addition, HSP70 was upregulated in human neuroblastoma cells (SK-N-SH) infected with EV71.
['Xu|Tiantian|T|', 'Lin|Zhengfang|Z|', 'Wang|Changbing|C|', 'Li|Yinghua|Y|', 'Xia|Yu|Y|', 'Zhao|Mingqi|M|', 'Hua|Liang|L|', 'Chen|Yi|Y|', 'Guo|Min|M|', 'Zhu|Bing|B|']
D002460:Cell Line D029821:Enterovirus A, Human D004769:Enterovirus Infections D055785:Gene Knockdown Techniques D018840:HSP70 Heat-Shock Proteins D054884:Host-Pathogen Interactions D006801:Humans D009447:Neuroblastoma D009474:Neurons D034741:RNA, Small Interfering D015854:Up-Regulation D053585:Virus Attachment D053586:Virus Internalization D014779:Virus Replication
Heat shock protein 70 Enterovirus 71 Hand Foot and mouth disease Nerve cells