In silico analysis of the chemotactic system of Agrobacterium tumefaciens.
Agrobacterium tumefaciens is an efficient tool for creating transgenic host plants. The first step in the genetic transformation process involves A. tumefaciens chemotaxis, which is crucial to the survival of A. tumefaciens in changeable, harsh and even contaminated soil environments. However, a systematic study of its chemotactic signalling pathway is still lacking. In this study, the distribution and classification of chemotactic genes in the model A. tumefaciens C58 and 21 other strains were annotated. Local blast was used for comparative genomics, and hmmer was used for predicting protein domains. Chemotactic phenotypes for knockout mutants of ternary signalling complexes in A. tumefaciens C58 were evaluated using a swim agar plate. A major cluster, in which chemotaxis genes were consistently organized as MCP (methyl-accepting chemotaxis protein), CheS, CheY1, CheA, CheR, CheB, CheY2 and CheD, was found in A. tumefaciens, but two coupling CheW proteins were located outside the 'che' cluster. In the ternary signalling complexes, the absence of MCP atu0514 significantly impaired A. tumefaciens chemotaxis, and the absence of CheA (atu0517) or the deletion of both CheWs abolished chemotaxis. A total of 465 MCPs were found in the 22 strains, and the cytoplasmic domains of these MCPs were composed of 38 heptad repeats. A high homology was observed between the chemotactic systems of the 22 A. tumefaciens strains with individual differences in the gene and receptor protein distributions, possibly related to their ecological niches. This preliminary study demonstrates the chemotactic system of A. tumefaciens, and provides some reference for A. tumefaciens sensing and chemotaxis to exogenous signals.
['Xu|Nan|N|', 'Wang|Mingqi|M|', 'Yang|Xiaojing|X|', 'Xu|Yujuan|Y|', 'Guo|Minliang|M|']
D016960:Agrobacterium tumefaciens D000595:Amino Acid Sequence D002633:Chemotaxis D003198:Computer Simulation D016680:Genome, Bacterial D000072236:Methyl-Accepting Chemotaxis Proteins D010802:Phylogeny D010944:Plants D016415:Sequence Alignment D015398:Signal Transduction
Agrobacterium tumefaciens chemotaxis methyl-accepting chemotaxis protein Che cluster