Preparation of gelatin nanoparticles by two step desolvation method for application in photodynamic therapy.
Gelatin nanoparticles have recently been receiving considerable attention because they offer a good option as release systems due to their low cost, biocompatibility, biodegradability and its application in several types of formulations. This study aim was to evaluate the potential application of gelatin nanoparticles entrapping a photosensitizer in Photodynamic Therapy. Gelatin nanoparticles were studied by steady-state techniques and the biological activity evaluated by in vitro MTT assay. The particles were spherical in shape exhibiting a 273 nm diameter with a low tendency to aggregate. The loading efficiency was 76%. Photosensitizer photophysical properties were shown to be preserved after GN encapsulation. The cells viability obtaining 85% cells death compared with control. The results demonstrate that gelatin nanoparticles can be successfully applied for photosensitizers encapsulation or other active drugs and be used as an optimal medium for a variety of bioactive materials, which can also be encapsulated by the proposed method.
['Carvalho|Janicy Arantes|JA|', 'Abreu|Alexandro Silva|AS|', 'Ferreira|Vitória Tonini Porto|VTP|', 'Gonçalves|Erika Peterson|EP|', 'Tedesco|Antonio Claudio|AC|', 'Pinto|Juliana Guerra|JG|', 'Ferreira-Strixino|Juliana|J|', 'Beltrame Junior|Milton|M|', 'Simioni|Andreza Ribeiro|AR|']
D000970:Antineoplastic Agents D001672:Biocompatible Materials D045744:Cell Line, Tumor D002470:Cell Survival D004337:Drug Carriers D004339:Drug Compounding D065546:Drug Liberation D005780:Gelatin D006801:Humans D007211:Indoles D054833:Isoindoles D008027:Light D053758:Nanoparticles D010316:Particle Size D010778:Photochemotherapy D017319:Photosensitizing Agents D013499:Surface Properties
Gelatin nanoparticles photodynamic therapy photosensitizer