A specific and biocompatible fluorescent sensor based on the hybrid of GFP chromophore and peptide for HSA detection.
It is still a challenge for clinical diagnostics to design non-toxic and selective sensors for a specific analyte in blood serum, which contains a large number of proteins, small molecules, and ions. Herein, H1, a synthetic hybrid of green fluorescent protein (GFP) chromophore and peptide for human serum albumin (HSA) detection via obvious fluorescence enhancement was reported. The fluorescence response was caused by the non-covalent binding between H1 and the subdomain IIA cavity of HSA, and this specific binding behavior can be confirmed by the decrease of fluorescence intensity of the sole tryptophan residue (Trp-214) located in the same subdomain. H1 showed remarkable sensitivity and selectivity toward HSA without interference from other proteins. A good linear relationship between the fluorescence intensity of H1 and HSA concentration from 0 to 67.0μgmL(-1) was obtained with the detection limit reaching as low as 198.6ngL(-1). Moreover, H1 was successfully utilized to detect trace HSA in healthy human urine (HU) and human blood serum (HBS), respectively. The resulting H1/HSA system also showed a satisfactory sensing ability toward anionic surfactant sodium dodecyl sulfate (SDS) through decreased fluorescence intensity with the detection limit of 3.48ngL(-1).
['Liao|Caiyun|C|', 'Li|Fangfang|F|', 'Huang|Shanshan|S|', 'Zheng|Baozhan|B|', 'Du|Juan|J|', 'Xiao|Dan|D|']
D000419:Albuminuria D000818:Animals D015374:Biosensing Techniques D002417:Cattle D049452:Green Fluorescent Proteins D006801:Humans D057230:Limit of Detection D010455:Peptides D012709:Serum Albumin D012710:Serum Albumin, Bovine D012967:Sodium Dodecyl Sulfate D013050:Spectrometry, Fluorescence
GFP chromophore HSA SDS BSA Fluorescence detection