Apoptosis-Inducing Activity and Antiproliferative Effect of Gossypin on PC-3 Prostate Cancer Cells.
AIM
The rapid growth, morbidity and mortality of prostate cancer, and the lack of effective treatment have attracted great interest of researchers to find novel cancer therapies aiming at the effect of gossypin on cell proliferation and apoptosis of PC-3 cells.


METHODS
The effect of gossypin on cell viability was determined using MTT assay at 5-100μg/ml and cisplatin (50μM) in a time-dependent manner in PC-3 cell lines. The expression levels of caspase-3 (CASP3) and caspase-9 (CASP9) for apoptosis and Nuclear Factor Kappa B (NFKB1) for survival, inflammation, and growth were evaluated by real-time PCR. Hoechst staining was used to analyze apoptosis.


RESULTS
Gossypin showed an anti-proliferative effect on PC3 cell line in a time- and dose-dependent manner. In addition, gossypin led to a significant increase in apoptosis genes (CASP3, CASP9) when compared to control while it caused a decrease in the level of NFKB1, which is accepted as apoptosis inhibitor (p<0.05) (cisplatin-like). Gossypin 50 and 100μM significantly induced apoptotic mechanism in PC-3 cells. However, no apoptotic or commonly stained nuclei have been observed in control group cells.


CONCLUSION
The results indicated that gossypin can be defined as a promising anticancer agent for PC-3 human prostate cancer cell line.
['Cinar|Irfan|I|']
D000970:Antineoplastic Agents D017209:Apoptosis D049109:Cell Proliferation D002470:Cell Survival D004305:Dose-Response Relationship, Drug D004354:Drug Screening Assays, Antitumor D005419:Flavonoids D006801:Humans D015394:Molecular Structure D000078722:PC-3 Cells D013329:Structure-Activity Relationship
Gossypin apoptosis MTT assay anticancer caspase prostate cancer cells (PC-3)