Short Chain Fatty Acid Acetate Protects against Ethanol-Induced Acute Gastric Mucosal Lesion in Mice.
Short chain fatty acids acetate and propionate have been demonstrated protective function in the intestinal mucosa. However, their impact on gastric mucosa has not yet been elucidated. The current study aimed to investigate the potential protective effects of acetate and propionate against ethanol-induced gastric mucosal lesion and the underlying mechanism in mice. ICR mice were orally treated with acetate and propionate, respectively, 30 min prior to the establishment of gastric mucosal injury model by challenge with absolute ethanol. The gastric samples were collected for the detection of oxidative, inflammatory and apoptotic related parameters. Acetate, but not propionate, attenuated the severity of gastric mucosal damage as evidenced by the gross changes of gastric mucosa, pathological aberrations. Acetate alleviated oxidative stress as shown by the increase in glutathione (GSH) content and superoxide dismutase (SOD) activities, and the decrease of malondialdehyde (MDA) level. The elevated concentrations of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-6, and the activation of nuclear factor-kappaB (NF-κB) p65 by ethanol stimulation was also reduced by acetate. Moreover, the anti-inflammatory factors, IL-4, LXA4 and IL-10, were up-regulated in acetate treated group. With respect to gastric mucosal apoptosis, acetate suppressed caspase-3 activity and BAX expression in favor of cell survival. These favorable actions were maybe associated with up-regulation of the gastric MUC5AC, the key defense factor of gastric mucosal system. These findings accentuate the gastroprotective actions of acetate in ethanol-induced gastric injury which were mediated via concerted multi-prolonged actions, including suppression of gastric oxidation, inflammation and apoptosis and promotion of MUC5AC expression.
['Liu|Jiaming|J|', 'Wang|Jindan|J|', 'Shi|Yilu|Y|', 'Su|Wei|W|', 'Chen|Jie|J|', 'Zhang|Zhengzheng|Z|', 'Wang|Guocan|G|', 'Wang|Fangyan|F|']
D000085:Acetates D000818:Animals D017209:Apoptosis D053148:Caspase 3 D002470:Cell Survival D002492:Central Nervous System Depressants D016207:Cytokines D000431:Ethanol D005232:Fatty Acids, Volatile D005753:Gastric Mucosa D007249:Inflammation D008297:Male D051379:Mice D008813:Mice, Inbred ICR D055271:Mucin 5AC D018384:Oxidative Stress D011422:Propionates D013276:Stomach Ulcer D013329:Structure-Activity Relationship
short chain fatty acid acetate gastric mucosa ethanol oxidative stress inflammation