Eribulin upregulates miR-195 expression and downregulates Wnt3a expression in non-basal-like type of triple-negative breast cancer cell MDA-MB-231.
Triple-negative breast cancer (TNBC), which does not show hormone sensitivity, is a poor prognosis disease without an established targeted treatment, so that establishing a therapeutic target for each subtype is desired. In addition, microRNA (miRNA), a non-cording RNA 19-25 nucleotide-longs in length, is known to be involved in regulating gene expression. We examined miRNA expression after exposure to eribulin, MDA-MB-231 cells, non-basal-like type of TNBC cell lines, and HCC1143 cells, basal-like type of TNBC cell lines. The activity of caspase-3 significantly increased compared to the control in MDA-MB-231, whereas no significant difference was observed in HCC1143. The expression level of 20-miRNAs significantly increased compared to the control in MDA-MB-231 after exposure to eribulin. The expression level of 6-miRNAs also significantly increased compared to the control in HCC1143. In these 2 cell types, miR-125b-1 and miR-195 were commonly expressed. While the expression level of miR-125b-1 decreased in both cells, the expression level of miR-195 increased in MDA-MB-231 and decreased in HCC1143. The expression level of miR-195 targeting Wnt3a significantly decreased compared to the control in MDA-MB-231, whereas it significantly increased in HCC1143. These results showed that exposure to eribulin highly increased the expression of miR-195 while it decreased the expression of Wnt3a in non-basal-like type of TNBC. Some miRNAs are known to regulate other signaling pathways involved in human pathogenesis by regulating the Wnt signaling pathway, and miRNA can act as a tumor-suppressing gene; therefore, miR-195 may serve as a therapeutic target in non-basal-like type of TNBC.
['Furuya|Kanji|K|', 'Sasaki|Akiko|A|', 'Tsunoda|Yuko|Y|', 'Tsuji|Mayumi|M|', 'Udaka|Yuko|Y|', 'Oyamada|Hideto|H|', 'Tsuchiya|Hiromichi|H|', 'Oguchi|Katsuji|K|']
D001943:Breast Neoplasms D045744:Cell Line, Tumor D015536:Down-Regulation D005260:Female D005663:Furans D015870:Gene Expression D016147:Genes, Tumor Suppressor D006801:Humans D007659:Ketones D035683:MicroRNAs D058990:Molecular Targeted Therapy D015398:Signal Transduction D015854:Up-Regulation D060509:Wnt3A Protein
Eribulin Wnt3a Breast cancer MicroRNA Triple negative