MEN1 Tumor Suppressor Gene is Required for Long-term Memory Formation in an Aversive Operant Conditioning Model of Lymnaea stagnalis.
Activity-dependent transcription factors critically coordinate the gene expression program underlying memory formation. The tumor suppressor gene, MEN1, encodes a ubiquitously expressed transcription regulator required for synaptogenesis and synaptic plasticity in invertebrate and vertebrate central neurons. In this study, we investigated the role of MEN1 in long-term memory (LTM) formation in an aversive operant conditioning paradigm in the freshwater pond snail Lymnaea stagnalis (L. stagnalis). We demonstrated that LTM formation is associated with an increased expression of MEN1 coinciding with an up-regulation of creb1 gene expression. In vivo knockdown of MEN1 prevented LTM formation and conditioning-induced changes in neuronal activity in the identified pacemaker neuron RPeD1. Our findings suggest the involvement of a new pathway in LTM consolidation that requires MEN1-mediated gene regulation.
['Dong|Nancy|N|', 'Senzel|Anthony|A|', 'Li|Kathy|K|', 'Lu|Tom Z|TZ|', 'Guo|Cong-Hui|CH|', 'Aleksic|Mila|M|', 'Feng|Zhong-Ping|ZP|']
D000200:Action Potentials D000595:Amino Acid Sequence D000818:Animals D001362:Avoidance Learning D003216:Conditioning, Operant D017362:Cyclic AMP Response Element-Binding Protein D055785:Gene Knockdown Techniques D008195:Lymnaea D057567:Memory, Long-Term D009474:Neurons D010802:Phylogeny D011518:Proto-Oncogene Proteins D016415:Sequence Alignment D017386:Sequence Homology, Amino Acid D015854:Up-Regulation
MEN1 Lymnaea stagnalis Creb1 RPeD1 aversive long-term memory electrophysiology