Biocompatible fluorescent carbon dots derived from roast duck for in vitro cellular and in vivo C. elegans bio-imaging.
Biocompatible fluorescent carbon dots (CDs) were prepared via a simple and green route using duck breasts as a natural carbon source. The CDs from duck breasts were well dispersed, and their mean particle size decreased from 2.59 to 1.95 nm when the roasting temperature increased from 200 to 300 °C. Abundant functional groups such as OH, COOH, and NH2 were observed on the surface of the CDs, providing the CDs with good water solubility. These CDs emitted strong fluorescence under ultraviolet light irradiation and exhibited superior photostability. The absolute fluorescence quantum yield of CDs rose from 10.53% to 38.05% when the relative nitrogen content of CDs increased from 7.18% to 12.73%. The CDs showed low toxicity to PC12 cells for prolonged exposure. Therefore, the duck CDs were successfully developed as fluorescent probes for in vitro PC12 cells and in vivo Caenorhabditis elegans imaging. These results indicated that the CDs derived from roast duck were biocompatible and can potentially be used as probes in bio-imaging.
['Cong|Shuang|S|', 'Liu|Kangjing|K|', 'Qiao|Fengzhi|F|', 'Song|Yukun|Y|', 'Tan|Mingqian|M|']
D000818:Animals D001672:Biocompatible Materials D017173:Caenorhabditis elegans D002244:Carbon D003296:Cooking D004372:Ducks D005453:Fluorescence D006863:Hydrogen-Ion Concentration D007477:Ions D009682:Magnetic Resonance Spectroscopy D008460:Meat D046529:Microscopy, Electron, Transmission D009584:Nitrogen D016716:PC12 Cells D011200:Poultry D045663:Quantum Dots D051381:Rats D012995:Solubility D013050:Spectrometry, Fluorescence D013056:Spectrophotometry, Ultraviolet D013696:Temperature
Carbon dots Roast duck Bio-imaging Fluorescent probes Formation