Diagnostic value of quantitative MP-IgG for Mycoplasma pneumoniae pneumonia in adults.
The passive particle agglutination (PA) test, once widely used for Mycoplasma pneumoniae (M. pneumoniae) antibody detection, has gradually been replaced by quantitative enzyme-linked immunosorbent assays (ELISA). However, the lack of diagnostic criteria for quantitative ELISA M. pneumoniae-IgG (MP-IgG) and the low positive rates of ELISA M. pneumoniae-IgM (MP-IgM) limit the diagnostic value of ELISA for M. pneumoniae infection in adults. Here, the diagnostic value of quantitative ELISA MP-IgG was evaluated in adults with Mycoplasma pneumoniae pneumonia (MPP). The serum M. pneumoniae antibodies were detected in 162 patients with MPP, 228 patients with community-acquired pneumonia (CAP) with non-Mycoplasma pneumoniae (NMP), and 162 healthy controls by ELISA, using the PA results as the reference standards. For the MP-IgM-/IgG+ subgroup, a single serum MP-IgG level of ≥92.67 RU/mL can be used as a reference criterion for the diagnosis of acute M. pneumoniae infection. At admission, for patients with CAP, the sensitivity and specificity of ELISA MP-IgM positivity for MPP were 18.51% and 99.56%, respectively. MP-IgM positivity combined with MP-IgG ≥ 92.67 RU/mL increased the sensitivity to 40.12% and decreased the specificity to 94.29%. For paired serum samples obtained within seven days, an ELISA MP-IgG concentration change of ≥1.48-fold and MP-IgG ≥ 92.67 RU/mL on day 7 were used as the diagnostic criteria for M. pneumoniae infection. Accordingly, the combination of qualitative MP-IgM detection and quantitative MP-IgG detection by ELISA is valuable for acute MPP diagnosis in adults.
['Wu|Lina|L|', 'Ye|Maosheng|M|', 'Qin|Xiaosong|X|', 'Liu|Yong|Y|', 'Lv|Zhe|Z|', 'Zheng|Rui|R|']
D000328:Adult D000907:Antibodies, Bacterial D016022:Case-Control Studies D004797:Enzyme-Linked Immunosorbent Assay D006801:Humans D007074:Immunoglobulin G D007075:Immunoglobulin M D009177:Mycoplasma pneumoniae D011019:Pneumonia, Mycoplasma D012680:Sensitivity and Specificity
Mycoplasma pneumoniae Enzyme-linked immunosorbent assay IgG IgM Passive particle agglutination test