MIPs-graphene nanoplatelets-MWCNTs modified glassy carbon electrode for the determination of cardiac troponin I.
An electrochemical sensor with high selectivity in addition to sensitivity was developed for the determination of cardiac troponin I (cTnI), based on the modification of cTnI imprinted polymer film on a glassy carbon electrode (GCE). The sensor was fabricated by layer-by-layer assembled graphene nanoplatelets (GS), multiwalled carbon nanotubes (MWCNTs), chitosan (CS), glutaraldehyde (GA) composites, which can increase the electronic transfer rate and the active surface area to capture a larger number of antigenic proteins. MWCNTs/GS based imprinted polymers (MIPs/MWCNTs/GS) were synthesized by means of methacrylic acid (MAA) as the monomer, ethylene glycol dimethacrylate (EGDMA) as the cross linker α,α'-azobisisobutyronitrile (AIBN) as the initiator and cTnI as the template. In comparison with conventional methods, the proposed electrochemical sensor is highly sensitive for cTnI, providing a better linear response range from 0.005 to 60 ng cm-3 and a lower limit of detection (LOD) of 0.0008 ng cm-3 under optimal experimental conditions. In addition, the electrochemical sensor exhibited good specificity, acceptable reproducibility and stability. Moreover, satisfactory results were obtained in real human serum samples, indicating that the developed method has the potential to find application in clinical detection of cTnI as an alternative approach.
['Ma|Ya|Y|', 'Shen|Xiao-Lei|XL|', 'Wang|Hai-Shui|HS|', 'Tao|Jia|J|', 'Huang|Jian-Zhi|JZ|', 'Zeng|Qiang|Q|', 'Wang|Li-Shi|LS|']
D002244:Carbon D055664:Electrochemical Techniques D004566:Electrodes D006108:Graphite D006801:Humans D057230:Limit of Detection D008855:Microscopy, Electron, Scanning D054802:Molecular Imprinting D009206:Myocardium D049329:Nanostructures D037742:Nanotubes, Carbon D011108:Polymers D015203:Reproducibility of Results D017550:Spectroscopy, Fourier Transform Infrared D019210:Troponin I
Electrochemical sensor Cardiac troponin I Graphene nanoplatelets Multiwalled carbon nanotubes Molecularly imprinted polymers