Suppression of CSN5 promotes the apoptosis of gastric cancer cells through regulating p53-related apoptotic pathways.
As one of the COP9 signalosome complex, CSN5 (also known as Jab1) has been confirmed overexpression in many human cancers and affected multiple pathways associating with cell proliferation and apoptosis. Correlation of CSN5 overexpression with poor prognosis for cancer provides evidence that it is involved in the tumorigenesis. However, little is known about the functional role and the underlying mechanism of CSN5 in gastric cancer progression. In the current study, the effect of CSN5 siRNA (small-interfering RNA) on the proliferation and apoptosis of human gastric cancer cells (AGS and MKN45) were examined. Our results showed that knockdown of CSN5 could inhibit proliferation and promote apoptosis of gastric cancer cells. Additionally, suppression of CSN5 expression contributed to the increased expression levels of p53 and Bax. In conclusion, CSN5 overexpression is significantly correlated with gastric cancer progression, and CSN5 could be a novel target in gastric cancer therapy.
['Sang|Miao-Miao|MM|', 'Du|Wen-Qi|WQ|', 'Zhang|Rui-Yan|RY|', 'Zheng|Jun-Nian|JN|', 'Pei|Dong-Sheng|DS|']
D017209:Apoptosis D000075686:COP9 Signalosome Complex D045744:Cell Line, Tumor D005753:Gastric Mucosa D015972:Gene Expression Regulation, Neoplastic D006801:Humans D047908:Intracellular Signaling Peptides and Proteins D010447:Peptide Hydrolases D034622:RNA Interference D034741:RNA, Small Interfering D015398:Signal Transduction D013270:Stomach D013274:Stomach Neoplasms D016159:Tumor Suppressor Protein p53 D051028:bcl-2-Associated X Protein
Apoptosis Gastric cancer Proliferation siRNA p53 CSN5/Jab1