A signal transduction approach for multiplexed detection of transcription factors by integrating DNA nanotechnology, multi-channeled isothermal amplification, and chromatography.
The variation patterns of transcription factors (TFs) provide direct information for the states of cell populations, which is of significance for biomedical research and clinical diagnostics. Herein, we show that through multi-channeled isothermal amplification, it is feasible to connect DNA-based signal transduction with chromatography for multiplexed detection of TFs. The described system is referred to as "PAC" which includes three major steps: (i) Protection, which uses DNA-modified magnetic beads to capture TFs and converts the capturing event into triggering signal; (ii) Amplification, which receives the triggering signal and generate DNA reporters through multi-channeled extension and nicking of oligonucleotides; and (iii) Chromatography, which separates and detects the DNA reporters in liquid chromatography. The quantitative detection of five essential TFs includes p50, p53, AP-1, MITF, and c-Myc is realized in a multiplexed manner, with the lowest detection limit of 0.5 pM. PAC can also provide effective means to measure the above five TFs in real samples, including cultured cells, xenograft tumors, and blood-based liquid biopsy. This study not only established a solution for multiplexed measurement of TFs for molecular diagnostics, but also paved avenue for bridging the gap between DNA nanotechnology and chromatography.
['Chen|Yue|Y|', 'Yan|Xiaoqiang|X|', 'Yang|Wei|W|', 'Wang|Jing|J|', 'Lu|Qiaoyun|Q|', 'Li|Bingzhi|B|', 'Zhu|Wanying|W|', 'Zhou|Xuemin|X|']
D000818:Animals D002478:Cells, Cultured D002853:Chromatography, Liquid D004247:DNA D015342:DNA Probes D006801:Humans D057230:Limit of Detection D000073890:Liquid Biopsy D051379:Mice D049329:Nanostructures D036103:Nanotechnology D021141:Nucleic Acid Amplification Techniques D009841:Oligonucleotides D014157:Transcription Factors D023041:Xenograft Model Antitumor Assays
Multiplexed detection Transcription factor DNA nanotechnology Isothermal amplification HPLC Magnetic separation