Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis.
Many studies have analyzed myelin-reactivity of T cells in multiple sclerosis (MS); however, with conflicting results. In this study we compare methods to determine myelin reactivity of T cells and aim to delineate the cause of inconsistency in the literature. Challenging T cells with myelin antigens we found a significant increase in antigen-reactivity of T cells from patients with MS using an ELISpot-assay, in contrast to a CFSE-dilution assay. Comparing the two assays showed that the myelin-reactive T cells detected in the ELISpot-assay originated primarily from effector memory T cells in contrast to the myelin-reactive T cells of the CFSE-assay representing a population of both naïve, central memory and effector memory T cells. This diversity in T cell populations activated in the two assays likely contribute to the discrepancy found in the literature and encourages thorough considerations when choosing an assay to determine antigen-specificity of T cells in future studies.
['von Essen|Marina Rode|MR|', 'Ammitzbøll|Cecilie|C|', 'Börnsen|Lars|L|', 'Sellebjerg|Finn|F|']
D000328:Adult D001324:Autoantigens D016022:Case-Control Studies D058501:Enzyme-Linked Immunospot Assay D005260:Female D005452:Fluoresceins D005456:Fluorescent Dyes D006801:Humans D007118:Immunoassay D007156:Immunologic Memory D008297:Male D008875:Middle Aged D020529:Multiple Sclerosis, Relapsing-Remitting D004676:Myelin Basic Protein D009185:Myelin Proteins D063308:Myelin-Oligodendrocyte Glycoprotein D013388:Succinimides D013601:T-Lymphocytes D055815:Young Adult
Multiple sclerosis Myelin-reactive T cells Assessing current methods