Long non-coding RNA-LET is a positive prognostic factor and exhibits tumor-suppressive activity in gallbladder cancer.
The identification of cancer-associated long non-coding RNAs (lncRNAs) and the investigation of their molecular and biological functions are vital for understanding the molecular biology and progression of cancer. The lncRNA-LET, a newly identified lncRNA, was demonstrated to be down-regulated in hepatocellular cancer. However, little is known about its role in gallbladder cancer. In the present study, an obvious down-regulation of lncRNA-LET was observed in gallbladder cancer compared to their adjacent normal tissues. Meanwhile, patients with low expression of lncRNA-LET have significantly poorer prognosis than those with high expression. We confirmed that hypoxia decreased lncRNA-LET levels in gallbladder cancer cells. Moreover, lncRNA-LET overexpression was further validated to inhibit the invasion of gallbladder cancer cells under hypoxic or normoxic conditions in vitro. We demonstrated that lncRNA-LET overexpression conferred a proliferative advantage to tumor cells under hypoxic conditions. The ectopic expression of lncRNA-LET led to the promotion of cell cycle arrest at G0/G1 phase and to the induction of apoptosis under hypoxic conditions. Ectopic expression of LncRNA-LET also suppressed gallbladder tumor growth in vivo. Our findings indicate that lncRNA-LET may represent a prognostic marker and a potential therapeutic target for gallbladder cancer.
['Ma|Ming-zhe|MZ|', 'Kong|Xiang|X|', 'Weng|Ming-zhe|MZ|', 'Zhang|Ming-di|MD|', 'Qin|Yi-yu|YY|', 'Gong|Wei|W|', 'Zhang|Wen-jie|WJ|', 'Quan|Zhi-wei|ZW|']
D000818:Animals D017209:Apoptosis D014408:Biomarkers, Tumor D045744:Cell Line, Tumor D015536:Down-Regulation D005260:Female D059585:G1 Phase Cell Cycle Checkpoints D005706:Gallbladder Neoplasms D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008297:Male D051379:Mice D008819:Mice, Nude D008875:Middle Aged D011379:Prognosis D062085:RNA, Long Noncoding D016192:Resting Phase, Cell Cycle
gallbladder cancer long non-coding RNA lncRNA-LET prognosis hypoxia