Sweet Cherry (Prunus avium L.) PaPIP1;4 Is a Functional Aquaporin Upregulated by Pre-Harvest Calcium Treatments that Prevent Cracking.
The involvement of aquaporins in rain-induced sweet cherry (Prunus avium L.) fruit cracking is an important research topic with potential agricultural applications. In the present study, we performed the functional characterization of PaPIP1;4, the most expressed aquaporin in sweet cherry fruit. Field experiments focused on the pre-harvest exogenous application to sweet cherry trees, cultivar Skeena, with a solution of 0.5% CaCl2, which is the most common treatment to prevent cracking. Results show that PaPIP1;4 was mostly expressed in the fruit peduncle, but its steady-state transcript levels were higher in fruits from CaCl2-treated plants than in controls. The transient expression of PaPIP1;4-GFP in tobacco epidermal cells and the overexpression of PaPIP1;4 in YSH1172 yeast mutation showed that PaPIP1;4 is a plasma membrane protein able to transport water and hydrogen peroxide. In this study, we characterized for the first time a plasma membrane sweet cherry aquaporin able to transport water and H2O2 that is upregulated by the pre-harvest exogenous application of CaCl2 supplements.
['Breia|Richard|R|', 'Mósca|Andreia F|AF|', 'Conde|Artur|A|0000-0001-7102-6695', 'Correia|Sofia|S|0000-0003-2222-6396', 'Conde|Carlos|C|0000-0002-4177-8519', 'Noronha|Henrique|H|', 'Soveral|Graça|G|0000-0001-8487-110X', 'Gonçalves|Berta|B|', 'Gerós|Hernâni|H|0000-0002-3040-4095']
D000595:Amino Acid Sequence D020346:Aquaporins D002118:Calcium D003001:Cloning, Molecular D019295:Computational Biology D005638:Fruit D010940:Plant Proteins D000068220:Prunus avium D017422:Sequence Analysis, DNA
aquaporins 4 PaPIP1 calcium application fruit-cracking sweet-cherry