Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice.
Three antigens (NcSAG1, NcSRS2 and NcMIC3) from Neospora caninum were expressed using the BmNPV bacmid system in silkworm larvae and purified from the hemolymph. From 20 silkworm larvae, 1.5, 1.2 and 1.4 mg of purified recombinant NcSAG1, NcSRS2 and NcMIC3 were obtained, respectively. When each purified recombinant antigen was immunized with Freund's incomplete adjuvant (FIA) to mice, recombinant NcSAG1 induced a Th2 immune response in immunized mice and produced a SAG1-specific antibody. In the experiment where NcSAG1-immunized mice were challenged with N. caninum, the cerebral N. caninum burden was significantly reduced compared with that of either the FIA- or PBS-immunized mice. Recombinant NcSRS2 or NcMIC3 induced both Th1 and Th2 immune responses, but NcMIC3-immunization did not induce significant production of NcMIC3-specific antibodies. These results suggest that the silkworm can produce recombinant antigens of N. caninum, which can be used as a recombinant vaccine against N. caninum.
['Yoshimoto|Mai|M|', 'Otsuki|Takahiro|T|', 'Itagaki|Kohei|K|', 'Kato|Tatsuya|T|', 'Kohsaka|Tetsuya|T|', 'Matsumoto|Yumino|Y|', 'Ike|Kazunori|K|', 'Park|Enoch Y|EY|']
D000818:Animals D000953:Antigens, Protozoan D012831:Bombyx D003048:Coccidiosis D005260:Female D006458:Hemolymph D007114:Immunization D007814:Larva D051379:Mice D008807:Mice, Inbred BALB C D018688:Neospora D016052:Protozoan Vaccines D011994:Recombinant Proteins D018417:Th1 Cells D018418:Th2 Cells D022223:Vaccines, Subunit
Antigen Silkworm BmNPV bacmid Neospora caninum Subunit vaccine