Nap1l1 Controls Embryonic Neural Progenitor Cell Proliferation and Differentiation in the Developing Brain.
The precise function and role of nucleosome assembly protein 1-like 1 (Nap1l1) in brain development are unclear. Here, we find that Nap1l1 knockdown decreases neural progenitor cell (NPC) proliferation and induces premature neuronal differentiation during cortical development. A similar deficiency in embryonic neurogenesis was observed in Nap1l1 knockout (KO) mice, which were generated using the CRISPR-Cas9 system. RNA sequencing (RNA-seq) analysis indicates that Ras-associated domain family member 10 (RassF10) may be the downstream target of Nap1l1. Furthermore, we found that Nap1l1 regulates RassF10 expression by promoting SETD1A-mediated H3K4 trimethylation at the RassF10 promoter. Nap1l1 KO defects may be rescued by RassF10 overexpression, suggesting that Nap1l1 controls NPC differentiation through RassF10. Our findings reveal an essential role for the Nap1l1 histone chaperone in cortical neurogenesis during early embryonic brain development.
['Qiao|Huimin|H|', 'Li|Yanxin|Y|', 'Feng|Chao|C|', 'Duo|Shuguang|S|', 'Ji|Fen|F|', 'Jiao|Jianwei|J|']
D000818:Animals D001921:Brain D002454:Cell Differentiation D049109:Cell Proliferation D002540:Cerebral Cortex D005260:Female D017353:Gene Deletion D018507:Gene Expression Regulation, Developmental D020868:Gene Silencing D011495:Histone-Lysine N-Methyltransferase D006657:Histones D008239:Lysine D008745:Methylation D008813:Mice, Inbred ICR D018345:Mice, Knockout D008938:Mitosis D058953:Neural Stem Cells D055495:Neurogenesis D056490:Nucleosome Assembly Protein 1 D011401:Promoter Regions, Genetic
Nap1l1 neural progenitor cell RassF10 histone chaperone H3K4me3 cortex development gain of function loss of function neural differentiation and proliferation