The effect of caffeic acid phenethyl ester and thymoquinone on otitis media with effusion in rats.
OBJECTIVE
In this study, we aimed to investigate the effect of CAPE and thymoquinone in experimental rat otitis media with effusion (OME) model.


METHODS
Intraoral approach of eustachian tube orifice cauterization were administered to 36 of 40 rats participating the study. After application of exclusion criterias, 22 rats with appropriate conditions were determined. Totally 26 rats (44 otitis model ears and 8 normal ears) were randomly divided into 5 groups. While group I was consisted of healthy rats, the other groups were consisted of rats with otitis model. Group I (saline + control group; n = 8 normal ears) and group II (saline + otitis model; n = 10 otitis model ears) received intraperitoneally saline solution. CAPE was given intraperitoneally to group III (CAPE + otitis model; n = 12 otitis model ears) at a concentration of 10 mg/kg for treatment of otitis media. Group IV (thymoquinone + otitis model; n = 12 otitis model ears) was treated orally with 10 mg/kg of thymoquinone. Group V (methylprednisolone + otitis model; n = 10 otitis model ears) was treated intraperitoneally with 1 mg/kg of methylprednisolone. Tympanic bulla samples were excised after 10th day of treatment and examined under light microscopy.


RESULTS
Submucosal neutrophil leukocyte count of group I was significantly lower than other groups (II, IV, V) (respectively p < 0,0001, p < 0,001, p < 0,0001, Tukey test), while it was not significantly different from group III (p = 0,056, Tukey test). Submucosal neutrophil leukocyte count of group III was significantly lower than group II and group V (p = 0.029 ve p = 0.03, Tukey test). There was no significant difference between group IV and group V (p = 0,28, Tukey test).


CONCLUSION
Based on these findings, it could be suggested that CAPE, anti inflammatory properties proven in the literature, plays an important role in OME treatment.
['Gülmez|Mehmet İhsan|Mİ|', 'Okuyucu|Şemsettin|Ş|', 'Dokuyucu|Recep|R|', 'Gökçe|Hasan|H|']
D000818:Animals D000893:Anti-Inflammatory Agents D016227:Benzoquinones D002109:Caffeic Acids D004195:Disease Models, Animal D005064:Eustachian Tube D007274:Injections, Intraperitoneal D008297:Male D010034:Otitis Media with Effusion D010626:Phenylethyl Alcohol D051381:Rats D017208:Rats, Wistar D013701:Temporal Bone
Thymoquinone Otitis media with effusion Caffeic acid phenyl ester Inflammation