The natural product fucoidan ameliorates hepatic ischemia-reperfusion injury in mice.
Fucoidan is a sulfated polysaccharide based predominantly on l-fucose, and has several biologic functions. Reactive oxygen species-mediated apoptosis and autophagy and release of related inflammatory factors have important roles in hepatic ischemia-reperfusion injury (IR). Here, the effect of fucoidan on hepatic IR was investigated. Mice were randomized into sham, IR, and fucoidan (20, 40mg/kg for 14days) groups. Samples were collected to assess biochemical indicators, hepatocyte damage and levels of proteins related to signaling pathways at different time points. Fucoidan had no effect on normal liver tissue, but inhibited the increases in alanine aminotransferase, aspartate transaminase, inflammatory factors, and the hepatocyte damage caused by IR. Also, apoptosis and autophagy via the activated JAK2/STAT1 pathway were attenuated by fucoidan to protect against hepatic injury. In conclusion, fucoidan ameliorates hepatic IR injury in mice via JAK2/STAT1-mediated apoptosis and autophagy. Inhibition of this pathway may be associated with reduced release of related inflammatory cytokines, especially interferon-γ.
['Li|Jingjing|J|', 'Zhang|QingHui|Q|', 'Li|Sainan|S|', 'Dai|Weiqi|W|', 'Feng|Jiao|J|', 'Wu|Liwei|L|', 'Liu|Tong|T|', 'Chen|Kan|K|', 'Xia|Yujing|Y|', 'Lu|Jie|J|', 'Zhou|Yingqun|Y|', 'Fan|Xiaoming|X|', 'Guo|Chuanyong|C|']
D000410:Alanine Transaminase D000818:Animals D017209:Apoptosis D001219:Aspartate Aminotransferases D001343:Autophagy D016207:Cytokines D004195:Disease Models, Animal D004305:Dose-Response Relationship, Drug D018836:Inflammation Mediators D007371:Interferon-gamma D053614:Janus Kinase 2 D008107:Liver Diseases D008297:Male D051379:Mice D008807:Mice, Inbred BALB C D011134:Polysaccharides D011897:Random Allocation D017382:Reactive Oxygen Species D015427:Reperfusion Injury D050794:STAT1 Transcription Factor
Fucoidan Hepatic ischemia reperfusion Interferon-gamma