Potential cytotoxic Diels-Alder type adducts from liquid medium of Morus Alba var. shalun root cultures.
A new Diels-Alder type adduct named morushalunin (1), together with three similar compounds, guangsangon E (2), chalcomoracin (3), and kuwanon J (4) were isolated from the EtOAc extract of liquid medium of Morus alba var. shalun root cultures. The structures of the isolated compounds were determined based on spectroscopic data, including 1D, 2D NMR, and MS data. Cytotoxic properties of compounds 1-4 were evaluated against murine leukemia P-388 cells. Morushalunin (1), guangsangon E (2), and chalcomoracin (3) were found to have significant cytotoxicity with IC50 values 0.7, 2.5, and 1.7 μg/mL, respectively, while kuwanon J (4) showed weak cytotoxicity (IC50 = 5.9 μg/mL).
['Fitriani|Rizki|R|', 'Happyana|Nizar|N|', 'Hakim|Euis Holisotan|EH|']
D000818:Animals D066241:Carbon-13 Magnetic Resonance Spectroscopy D016923:Cell Death D045744:Cell Line, Tumor D061565:Cycloaddition Reaction D051379:Mice D031625:Morus D064209:Phytochemicals D018517:Plant Roots D066244:Proton Magnetic Resonance Spectroscopy
cytotoxicity liquid medium Morus Morus alba var. shalun Root cultures Diels-Alder adducts