Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus.
Porcine epidemic diarrhea virus (PEDV) is an important pig pathogen that can cause vomiting, diarrhea, and dehydration, leading to serious damage to the swine industry worldwide. In this study, a nanoparticle-assisted polymerase chain reaction (nanoPCR) assay targeting the N gene of PEDV was developed and the sensitivity and specificity were investigated. Under the optimized conditions for detection of PEDV RNA, the nanoPCR assay was 100-fold more sensitive than a conventional RT-PCR assay. The lower detection limit of the nanoPCR assay was 2.7 × 10(-6) ng/μL of PEDV RNA and no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nanoPCR assay for the detection of PEDV. The sensitive and specific nanoPCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of PEDV-infection.
['Yuan|Wanzhe|W|', 'Li|Yanan|Y|', 'Li|Peng|P|', 'Song|Qinye|Q|', 'Li|Limin|L|', 'Sun|Jiguo|J|']
D000818:Animals D018352:Coronavirus Infections D017931:DNA Primers D025202:Molecular Diagnostic Techniques D053758:Nanoparticles D019590:Nucleocapsid Proteins D016133:Polymerase Chain Reaction D053485:Porcine epidemic diarrhea virus D012680:Sensitivity and Specificity D013552:Swine D013553:Swine Diseases D014730:Veterinary Medicine
Detection Porcine epidemic diarrhea virus (PEDV) nanoPCR RT-PCR