UHRF1 is regulated by miR-124-3p and promotes cell proliferation in intrahepatic cholangiocarcinoma.
Ubiquitin-like with PHD and ring finger domains 1 (UHRF1) is abnormally overexpressed in multiple cancers and closely correlated with tumor-promoting effects, such as high proliferation. However, how UHRF1 functions in intrahepatic cholangiocarcinoma (ICC) has not yet been determined. Herein, we found that UHRF1 is overexpressed in ICC tissues. Downregulated UHRF1 attenuated the transition of the G1/S cell cycle and then suppressed cell proliferation in vitro and tumor growth in vivo. Moreover, upstream regulators of the UHRF1 expression were predicted, and we found that direct binding of miR-124-3p inhibited the UHRF1 expression. Elevated miR-124-3p suppressed proliferation and led to the arrest of the cell cycle. Furthermore, the expression of UHRF1 was positively correlated with PCNA. Clinically, we showed that elevated UHRF1 was associated with poor prognosis, and served as an independent prognostic factor in ICC patients. Together, these findings demonstrate that UHRF1, regulated by miR-124-3p, acts as a tumor promoter by promoting cell proliferation in ICC.
['Zhu|Mengxuan|M|0000-0002-0971-6027', 'Wei|Chuanyuan|C|', 'Lin|Jiajia|J|', 'Dong|Shuangshuang|S|', 'Gao|Dongmei|D|', 'Chen|Jie|J|', 'Zhao|Yan|Y|', 'Liu|Binbin|B|']
D000818:Animals D001483:Base Sequence D022762:CCAAT-Enhancer-Binding Proteins D002453:Cell Cycle D045744:Cell Line, Tumor D049109:Cell Proliferation D018281:Cholangiocarcinoma D015536:Down-Regulation D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008297:Male D008807:Mice, Inbred BALB C D008819:Mice, Nude D035683:MicroRNAs D008875:Middle Aged D015999:Multivariate Analysis D011379:Prognosis D018809:Proliferating Cell Nuclear Antigen D044767:Ubiquitin-Protein Ligases
UHRF1 miR-124-3p proliferation ICC cell cycle prognosis