Hydroxyethyl methacrylate grafted carboxy methyl tamarind (CMT-g-HEMA) polysaccharide based matrix as a suitable scaffold for skin tissue engineering.
Patho-physiologies related to skin are diverse in nature such as burns, skin ulcers, atopic dermatitis, psoriasis etc. which impose severe bio-medical problems and thus enforce requirement of new and healthy skin prepared through tissues engineering methodologies. However, fully functional and biodegradable matrix for attachment, growth, proliferation and differentiation of the relevant cells is not available. In the present study, we introduce a set of hydrogels synthesized by incorporation of a synthetic monomer (Hydroxyethlmethacryate) with a semi-synthetic polymer backbone (carboxy methyl tamarind, CMT) in different mole ratios. We termed these materials as CMT:HEMA based hydrogels and these were characterized by different physico-chemical techniques, namely by X-Ray Diffraction, SEM and Dynamic Light Scattering. Biocompatibility studies with HaCaT, NIH-3T3 and mouse dermal fibroblasts confirm that this material is biocompatible. MTT assay further confirmed that this material does not have any cytotoxic effects. Assays for mitochondrial functionality such as ATP assay and mitochondrial reactive oxygen (ROS) generation also suggest that this material is safe and does not have any cytotoxicity. Hemolytic assay with red blood cells and acute skin irritation test on SD Rats confirmed that this material is suitable for ex-vivo application in future. We suggest that this hydrogel is suitable for in-vivo applications and may have clinical and commercial importance against skin disorders.
['Choudhury|Priyanka|P|', 'Kumar|Satish|S|', 'Singh|Abhishek|A|', 'Kumar|Ashutosh|A|', 'Kaur|Navneet|N|', 'Sanyasi|Sridhar|S|', 'Chawla|Saurabh|S|', 'Goswami|Chandan|C|', 'Goswami|Luna|L|']
D000818:Animals D045744:Cell Line, Tumor D002478:Cells, Cultured D005260:Female D006801:Humans D008297:Male D008689:Methacrylates D011134:Polysaccharides D051381:Rats D017207:Rats, Sprague-Dawley D017382:Reactive Oxygen Species D023822:Tissue Engineering D054457:Tissue Scaffolds D014961:X-Ray Diffraction
Polysaccharide Skin tissue engineering Hydrogels Bio-medical application