Analysis of cancer-related mutations in extracellular vesicles RNA by Droplet Digital™ PCR.
Extracellular vesicles (EVs) are taking their place as potential biomarkers in the field of liquid biopsy. In this study, EVs were isolated from plasma samples of 31 patients with colorectal cancer and melanoma via differential centrifugation and Droplet Digital™ PCR (Bio-Rad, CA, USA) was used to profile BRAF V600E/K, KRAS G12A/C/D/V and KRAS G13D mutations from EV-derived cDNA. The concordance rates with corresponding tissue were 54% and 44% in the colorectal cancer and melanoma cohort, respectively. Two patients displayed mutations in EVs not previously detected in tissue as evidence for emerging molecular resistance to anti-EGFR and BRAF/MEK inhibitor therapy prior to radiological evidence of tumor progression. We concluded that EV-derived nucleic acids may provide clinically relevant diagnostic information and mirror evolution of the disease.
['Yap|Soo Ann|SA|0000-0002-9450-9822', 'Münster-Wandowski|Agnieszka|A|', 'Nonnenmacher|Anika|A|', 'Keilholz|Ulrich|U|', 'Liebs|Sandra|S|']
D014408:Biomarkers, Tumor D015179:Colorectal Neoplasms D000067128:Extracellular Vesicles D005260:Female D006801:Humans D000073890:Liquid Biopsy D008297:Male D008875:Middle Aged D009154:Mutation D016133:Polymerase Chain Reaction D011518:Proto-Oncogene Proteins D012313:RNA
extracellular vesicles liquid biopsy colorectal cancer melanoma ddPCR exosomes