The role of cell cycle progression for the apoptosis of cancer cells induced by palladium(II)-saccharinate complexes of terpyridine.
OBJECTIVES
Palladium complexes are potent and less toxic molecules in comparison to other metal based agents. Here, we characterized two palladium(II) saccharinate complexes with terpyridine for their cell cycle specificity.


MATERIALS AND METHODS
Cells were arrested at G1, G1/S boundary or mitosis using mimosine, double-Thymidine block, aphidicolin, nocodazole or colcemid, and evaluated based on morphology and flow cytometry. Synchronized cells were treated with the Pd(II) complexes, and viability was measured via MTT assay.


RESULTS
While treatment of arrested cells with the Pd(II) complexes resulted in no significant change in cell death in HCT-116 and MDA-MB-231 cells, HeLa cells were more sensitive in S/G1. The main form of cell death was found to be apoptosis.


CONCLUSIONS
Pd(II) complexes appear to be cell-cycle non-specific, while cell line dependent differences may be observed. Cells die through apoptosis regardless of the cell cycle stage, which makes these complexes more promising as anti-cancer agents.
['Kacar|Omer|O|', 'Cevatemre|Buse|B|', 'Hatipoglu|Ibrahim|I|', 'Arda|Nazli|N|', 'Ulukaya|Engin|E|', 'Yilmaz|Veysel T|VT|', 'Acilan|Ceyda|C|']
D000970:Antineoplastic Agents D017209:Apoptosis D002453:Cell Cycle D045744:Cell Line, Tumor D056831:Coordination Complexes D004354:Drug Screening Assays, Antitumor D006801:Humans D008856:Microscopy, Fluorescence
Apoptosis Cancer Cell cycle specific Cell cycle arrest Cytotoxicity Metal based anticancer agents Palladium(II) complex