Pseudomonas aeruginosa mannose-sensitive hemagglutinin inhibits proliferation and invasion via the PTEN/AKT pathway in HeLa cells.
We investigated the effects of Pseudomonas aeruginosa mannose-sensitive hemagglutinin (PA-MSHA) on the proliferation and invasion of human cervical cancer cell lines, as well as the molecular pathways underlying these effects. MTT cell proliferation assays revealed a time- and concentration-dependent cytotoxic effect of PA-MSHA on HeLa cells but not H8 cells. Flow cytometry with propidium iodide and annexin-V-fluorescein isothiocyanate labeling (FITC) indicated that various concentrations of PA-MSHA could induce apoptosis and G2-M cell cycle arrest in HeLa cells. PA-MSHA also impaired the migration and invasion abilities of HeLa cells in Wound healing and Transwell invasion assays. Western blot results demonstrated that PA-MSHA reduced the expression of p-AKT, p-GSK3β, BCL-2, Vimentin and β-catenin, but increased the levels of PTEN, BAD, BAX and E-cadherin in HeLa cells. Importantly, PTEN siRNA induced the activity of p-AKT, while PA-MSHA partly inhibited this induction, indicating that PA-MSHA may reduce the cell proliferation and invasion potential by activating PTEN and thus inhibiting the AKT pathway in vitro. These data suggest the potential application of PA-MSHA to the treatment of human cervical cancer.
['Yin|Tie-Qiu|TQ|', 'Ou-Yang|Xuan|X|', 'Jiao|Fang-Yan|FY|', 'Huang|Lu-Ping|LP|', 'Tang|Xu-Dong|XD|', 'Ren|Bi-Qiong|BQ|']
D015703:Antigens, CD D017209:Apoptosis D015820:Cadherins D002465:Cell Movement D049109:Cell Proliferation D005260:Female D036781:Fimbriae Proteins D000071679:Glycogen Synthase Kinase 3 beta D006367:HeLa Cells D006801:Humans D008358:Mannose D009361:Neoplasm Invasiveness D051059:PTEN Phosphohydrolase D010766:Phosphorylation D051057:Proto-Oncogene Proteins c-akt D019253:Proto-Oncogene Proteins c-bcl-2 D011550:Pseudomonas aeruginosa D034622:RNA Interference D034741:RNA, Small Interfering D011994:Recombinant Proteins D015398:Signal Transduction D002583:Uterine Cervical Neoplasms D014746:Vimentin D051028:bcl-2-Associated X Protein D051025:bcl-Associated Death Protein D051176:beta Catenin
PTEN PA-MSHA cervical cancer p-AKT p-GSK3β