Alternative splicing of VEGFA, APP and NUMB genes in colorectal cancer.
AIM
To investigate alternative splicing in vascular endothelial growth factor A (VEGFA), amyloid beta precursor protein (APP), and Numb homolog (NUMB) in colorectal cancer (CRC).


METHODS
Real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and PCR-restriction fragment length polymorphism analyses were performed to detect the expression of VEGFA, APP, and NUMB mRNA in 20 CRC tissues and matched adjacent normal tissues, as well as their alternative splicing variants.


RESULTS
qRT-PCR analysis revealed that the expression of APP, NUMB, and VEGFA165b mRNA were significantly downregulated, while VEGFA mRNA was upregulated, in CRC tissues (all P < 0.05). PCR-restriction fragment length polymorphism analysis revealed that the expression of VEGFA165a/b in CRC tissues was significantly higher than in adjacent normal tissues (P < 0.05). Compared with adjacent normal tissues, the expression of NUMB-PRR(S) in CRC tissues was significantly decreased (P < 0.05), and the expression of NUMB-PRR(L) was increased (P < 0.05).


CONCLUSION
Alternative splicing of VEGFA, APP, and NUMB may regulate the development of CRC, and represent new targets for its diagnosis, prognosis, and treatment.
['Zhao|Yi-Jun|YJ|', 'Han|Hua-Zhong|HZ|', 'Liang|Yong|Y|', 'Shi|Chen-Zhang|CZ|', 'Zhu|Qing-Chao|QC|', 'Yang|Jun|J|']
D000230:Adenocarcinoma D017398:Alternative Splicing D054458:Amplified Fragment Length Polymorphism Analysis D016564:Amyloid beta-Protein Precursor D015179:Colorectal Neoplasms D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D008297:Male D008565:Membrane Proteins D009419:Nerve Tissue Proteins D012150:Polymorphism, Restriction Fragment Length D012333:RNA, Messenger D060888:Real-Time Polymerase Chain Reaction D020133:Reverse Transcriptase Polymerase Chain Reaction D042461:Vascular Endothelial Growth Factor A
Alternative splicing Colorectal cancer Vascular endothelial growth factor A Amyloid beta precursor protein Numb homolog