Application of CRISPR-Cas9 for Long Noncoding RNA Genes in Cancer Research.
Long noncoding RNAs (LncRNA), a class of transcripts with lengths >200 nt, play a master role in the regulation of cancer pathogenesis. Recently, the CRISPR-Cas9 system has been explored as a revolutionary genome editing tool for molecular biology. Growing evidence shows that LncRNAs can be targeted by the CRISPR-Cas9 system used for evaluating its function. Thus, the CRISPR-Cas9 systems provide a novel gene-editing strategy for the modification of LncRNA expression. This review summarizes current knowledge of the functions and underlying mechanisms of LncRNA by CRISPR-Cas9. Emerging strategies for non-viral/viral delivery of CRISPR-Cas9 in a clinical context are also discussed.
['Zhen|Shuai|S|', 'Li|Xu|X|']
D000818:Animals D014408:Biomarkers, Tumor D064113:CRISPR-Cas Systems D002471:Cell Transformation, Neoplastic D000072669:Gene Editing D015972:Gene Expression Regulation, Neoplastic D053263:Gene Regulatory Networks D018014:Gene Transfer Techniques D015316:Genetic Therapy D006801:Humans D009369:Neoplasms D034622:RNA Interference D062085:RNA, Long Noncoding D012106:Research
CRISPR-Cas9 cancer LncRNA