Ribosome Display: A Potent Display Technology used for Selecting and Evolving Specific Binders with Desired Properties.
Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achievements for over 20 years. Compared with other related display techniques, ribosome display shows unique advantages and development prospects. This tool has been successfully exploited for the selection of functional and specific binders in vitro. This review provides a comprehensive survey of the applications of ribosome display in screening or evolving functional proteins as well as in diagnostics and therapeutics. Previous papers on ribosome display failed to comprehensively review evolutionary strategies for proteins. In the present paper, we review all existing evolutionary strategies that have been combined with ribosome display. We also discuss shortcomings, improvement strategies, and research tendency.
['Li|Ruowei|R|', 'Kang|Guangbo|G|', 'Hu|Min|M|', 'Huang|He|H|']
D000818:Animals D002474:Cell-Free System D002478:Cells, Cultured D004926:Escherichia coli D057166:High-Throughput Screening Assays D006801:Humans D025202:Molecular Diagnostic Techniques D015202:Protein Engineering D012270:Ribosomes
Ribosome display Display technologies Diagnostics Therapeutics Evolutionary strategies Affinity maturation Stability optimization