Medial Septal NMDA Glutamate Receptors are Involved in Modulation of Blood Natural Killer Cell Activity in Rats.
The purpose of the present study was to determine the specific role of the medial septal (MS) NMDA glutamate receptors on peripheral blood natural killer cell cytotoxicity (NKCC) and their (large granular lymphocyte, LGL) number, as well as the plasma concentration of tumor necrosis factor α (TNF-α) and corticosterone in male Wistar rats exposed to elevated plus maze (EPM) stress or non-stress conditions. The NMDA groups were injected with NMDA glutamate receptor agonist (N-methyl-D-aspartate; 0.25 μg/rat), the D-AP7 group was injected with DL-2-amino-7-phosphoheptanoate (0.1 μg/rat), an antagonist of NMDA glutamate receptors, and the control Sal group with saline (0.5 μl/rat) via previously implanted cannulae into the MS. There was an increase in the NKCC, NK/LGL number and plasma TNF-α concentration after the NMDA injections, being much stronger within the rats under non-stress conditions rather than the rats exposed to EPM stress. These parameters were decreased in the D-AP7 rats, suggesting receptor/ion channel specificity. Moreover, a lower plasma corticosterone concentration within the NMDA rather than the Sal and D-AP7 groups was found. The obtained results suggest that activation of the NMDA glutamate receptors in the MS, accompanied by changes in the corticosterone and cytokine responses, may be involved in modulation of the blood natural anti-tumor response, under EPM stress and non-stress conditions.
['Podlacha|Magdalena|M|', 'Glac|Wojciech|W|', 'Listowska|Magdalena|M|', 'Grembecka|Beata|B|', 'Majkutewicz|Irena|I|', 'Myślińska|Dorota|D|', 'Plucińska|Karolina|K|', 'Jerzemowska|Grażyna|G|', 'Grzybowska|Maria|M|', 'Wrona|Danuta|D|']
D000818:Animals D003345:Corticosterone D003602:Cytotoxicity, Immunologic D004797:Enzyme-Linked Immunosorbent Assay D018690:Excitatory Amino Acid Agonists D018691:Excitatory Amino Acid Antagonists D007276:Injections, Intraventricular D007694:Killer Cells, Natural D008297:Male D018782:Maze Learning D015213:Neuroimmunomodulation D051381:Rats D017208:Rats, Wistar D016194:Receptors, N-Methyl-D-Aspartate D012686:Septal Nuclei D013315:Stress, Psychological D014409:Tumor Necrosis Factor-alpha
Medial septum, elevated plus maze, anxiety, NK cells, tumor necrosis factor-α NMDA receptors