Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase.
This article reports experimental data related to the research article entitled "Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase" (R.R. Garafutdinov, A.R. Sakhabutdinova, M.S. Kupryushkin, D.V. Pyshnyi, 2020) [1]. Here, multimerization efficiency in terms of Tt (time-to-threshold) values obtained for artificial DNA templates with the different nucleotide sequences during isothermal amplification with Bst exo- DNA polymerase is given. Data on the influence of phosphoryl guanidine primers (PGO) on multimerization for the LTc template which has shown high efficiency of multimerization are presented as well.
['Garafutdinov|Ravil R|RR|', 'Sakhabutdinova|Assol R|AR|', 'Kupryushkin|Maxim S|MS|', 'Pyshnyi|Dmitrii V|DV|']
Multimerization Isothermal amplification Bst exo- DNA polymerase Phosphoryl guanidine oligonucleotides (PGO)