Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins.
Antibody-fusion proteins with ligands, e.g., of the TNF superfamily (TNFSF) can be adequately produced in mammalian expression systems. Here, we describe the transient production in adherent and suspension human embryonic kidney cells at laboratory scale, followed by purification procedures applying protein A and immobilized metal affinity chromatography for proteins with Fc domain and 6 × histidine-tag, respectively. In addition, characterization of the purified proteins by size exclusion chromatography is described.
['Siegemund|Martin|M|', 'Beha|Nadine|N|', 'Müller|Dafne|D|']
D000906:Antibodies D002846:Chromatography, Affinity D002850:Chromatography, Gel D057809:HEK293 Cells D006639:Histidine D006801:Humans D008024:Ligands D009842:Oligopeptides D011993:Recombinant Fusion Proteins D048069:Tumor Necrosis Factors
Antibody-fusion protein Protein A Size exclusion chromatography Fc fusion IMAC