Possible basis for the emergence of H1N1 viruses with pandemic potential from avian hosts.
Influenza A viruses of the H1N1 subtype have emerged from the avian influenza gene pool in aquatic birds and caused human pandemics at least twice during the past century. Despite this fact, surprisingly little is known about the H1N1 gene pool in the aquatic bird reservoir. A preliminary study showed that an H1N1 virus from a shorebird of the Charadriiformes order was transmitted between animals through the airborne route of infection, whereas an H1N1 virus from a bird of the Anseriformes order was not. Here we show that two of the three H1N1 viruses isolated from Charadriiformes species in 2009 were transmitted between animals through the airborne route of infection, and five H1N1 isolates from Anseriformes species were not. The one H1N1 virus from a Charadriiformes species that failed to transmit through the airborne route was a reassortant possessing multiple internal gene segments from Anseriformes species. The molecular differences between the airborne-transmissible and non-airborne-transmissible H1N1 viruses were multigenic, involving the selection of virus with human-like receptor-binding specificity (α2-6 sialic acid) and multiple differences in the polymerase complex, mainly in the PB2, PB1-F2, and nonstructural genes.
['Koçer|Zeynep A|ZA|', 'Krauss|Scott|S|', 'Zanin|Mark|M|', 'Danner|Angela|A|', 'Gulati|Shelly|S|', 'Jones|Jeremy C|JC|', 'Friedman|Kimberly|K|', 'Graham|Allison|A|', 'Forrest|Heather|H|', 'Seiler|Jon|J|', 'Air|Gillian M|GM|', 'Webster|Robert G|RG|']
D000391:Air Microbiology D000416:Alberta D025041:Animal Migration D000818:Animals D046090:Anseriformes D046091:Charadriiformes D004195:Disease Models, Animal D004197:Disease Reservoirs D005289:Ferrets D015321:Gene Rearrangement D016679:Genome, Viral D053118:Influenza A Virus, H1N1 Subtype D005585:Influenza in Birds D008297:Male D009296:Nasal Cavity D009515:New Jersey D010802:Phylogeny D011134:Polysaccharides D014779:Virus Replication
H1N1 avian Charadriiformes Anseriformes ferret model transmission