The order of concurrent training affects mTOR signaling but not mitochondrial biogenesis in mouse skeletal muscle.
Concurrent training involves a combination of two different modes of training. In this study, we conducted an experiment by combining resistance and endurance training. The purpose of this study was to investigate the influence of the order of concurrent training on signal molecules in skeletal muscle. The phosphorylation levels of p70 S6 kinase, S6 ribosomal protein, and 4E-binding protein 1, which are related to hypertrophy signaling, increased significantly in the resistance-endurance order group as compared with in control group not the endurance-resistance order group. The gene expressions related to metabolism were not changed by the order of concurrent training. The mitochondrial respiratory chain complex was evaluated by western blot. Although both groups of concurrent training showed a significant increase in MTCO1, UQCRC2, and ATP5A protein levels, we could not detect a difference based on the order of concurrent training. In conclusion, a concurrent training approach involving resistance training before endurance training on the same day is an effective way to activate both mTOR signaling and mitochondria biogenesis.
['Shirai|Takanaga|T|', 'Aoki|Yuki|Y|0000-0002-9211-6718', 'Takeda|Kohei|K|', 'Takemasa|Tohru|T|0000-0002-9591-2250']
D000818:Animals D008297:Male D051379:Mice D008813:Mice, Inbred ICR D008931:Mitochondria, Muscle D018482:Muscle, Skeletal D001678:Organelle Biogenesis D010766:Phosphorylation D010805:Physical Conditioning, Animal D051057:Proto-Oncogene Proteins c-akt D055070:Resistance Training D058570:TOR Serine-Threonine Kinases
concurrent training mTOR signaling mitochondrial biogenesis skeletal muscle