The circular RNA of peripheral blood mononuclear cells: Hsa_circ_0005836 as a new diagnostic biomarker and therapeutic target of active pulmonary tuberculosis.
It has been reported that circular RNA (circRNA) is associated with human cancer. However, few studies have been reported in active pulmonary tuberculosis (APTB). The global circRNA expression was detected in the peripheral blood mononuclear cells (PBMCs) of APTB patients (n=5) and health controls (HC) (n=5) by using high-throughput sequencing. According to the systematical bioinformatics analysis, the basic content of circRNAs and their fold changes in the two groups were calculated. We selected 6 significant differentially expressed circRNAs, hsa_circ_0005836, hsa_circ_0009128, hsa_circ_0003519, hsa_circ_0023956, hsa_circ_0078768, and hsa_circ_0088452 and validated the expression in PBMCs from APTB (n=10) and HC (n=10) by real-time quantitative reverse transcription-polymerase chain reactions (qRT-PCRs). Further, the verification of these specific circRNAs (hsa_circ_0005836 and hsa_circ_0009128) between APTB (n=34) and HC (n=30) in PBMCs was also conducted by qRT-PCRs. The RNA-seq data showed the significant differential expression of the 523 circRNAs between the APTB and HC groups (199 circRNAs were significantly up-regulated and 324 circRNAs were down-regulated). Hsa_circ_0005836 and hsa_circ_0009128 expression was significantly down-regulated in the PBMCs of APTB (P<0.05) in the samples of APTB compared to HC in our study. The gene ontology based enrichment analysis of the circRNA-miRNA-mRNAs network showed that cellular catabolic process (P=7.10E-08), regulation of metabolic process (P=2.10E-06), catalytic activity (P=3.67E-08), protein binding (P=1.71E-07), cell part (P=3.46E-06), intracellular part (P=1.71E-07), and intracellular (P=3.67E-08) were recognized in the comparisons between APTB and HC. Based on KEGG analysis, HTLV-I infection, regulation of actin cytoskeleton, neurotrophin signaling pathway and mTOR signaling pathway were relevant during tuberculosis bacillus infection. We found for the first time that hsa_circ_0005836 and hsa_circ_0009128 were significantly down-regulated in the PBMCs of APTB compared with HC. Our findings indicate hsa_circ_0005836 might serve as a novel potential biomarker for TB infection.
['Zhuang|Ze-Gang|ZG|', 'Zhang|Jun-Ai|JA|', 'Luo|Hou-Long|HL|', 'Liu|Gan-Bin|GB|', 'Lu|Yuan-Bin|YB|', 'Ge|Nan-Hai|NH|', 'Zheng|Bi-Ying|BY|', 'Li|Rui Xi|RX|', 'Chen|Chen|C|', 'Wang|Xin|X|', 'Liu|Yu-Qing|YQ|', 'Liu|Feng-Hui|FH|', 'Zhou|Yong|Y|', 'Cai|Xiao-Zhen|XZ|', 'Chen|Zheng W|ZW|', 'Xu|Jun-Fa|JF|']
D008841:Actin Cytoskeleton D000293:Adolescent D000328:Adult D000368:Aged D001483:Base Sequence D005260:Female D005819:Genetic Markers D059014:High-Throughput Nucleotide Sequencing D006801:Humans D007963:Leukocytes, Mononuclear D008297:Male D008875:Middle Aged D011134:Polysaccharides D012313:RNA D000079962:RNA, Circular D060888:Real-Time Polymerase Chain Reaction D017423:Sequence Analysis, RNA D015398:Signal Transduction D058570:TOR Serine-Threonine Kinases D014397:Tuberculosis, Pulmonary D055815:Young Adult
Circular RNA Biomarker Active pulmonary tuberculosis RNA sequence