Deficiency in the nuclear long noncoding RNA Charme causes myogenic defects and heart remodeling in mice.
Myogenesis is a highly regulated process that involves the conversion of progenitor cells into multinucleated myofibers. Besides proteins and miRNAs, long noncoding RNAs (lncRNAs) have been shown to participate in myogenic regulatory circuitries. Here, we characterize a murine chromatin-associated muscle-specific lncRNA, Charme, which contributes to the robustness of the myogenic program in vitro and in vivo In myocytes, Charme depletion triggers the disassembly of a specific chromosomal domain and the downregulation of myogenic genes contained therein. Notably, several Charme-sensitive genes are associated with human cardiomyopathies and Charme depletion in mice results in a peculiar cardiac remodeling phenotype with changes in size, structure, and shape of the heart. Moreover, the existence of an orthologous transcript in human, regulating the same subset of target genes, suggests an important and evolutionarily conserved function for Charme Altogether, these data describe a new example of a chromatin-associated lncRNA regulating the robustness of skeletal and cardiac myogenesis.
['Ballarino|Monica|M|', 'Cipriano|Andrea|A|', 'Tita|Rossella|R|', 'Santini|Tiziana|T|', 'Desideri|Fabio|F|', 'Morlando|Mariangela|M|', 'Colantoni|Alessio|A|', 'Carrieri|Claudia|C|', 'Nicoletti|Carmine|C|', 'Musarò|Antonio|A|', "Carroll|Dònal O'|DO|0000-0002-8626-2217", 'Bozzoni|Irene|I|0000-0002-3485-8537']
D000818:Animals D006801:Humans D051379:Mice D024510:Muscle Development D018485:Muscle Fibers, Skeletal D032383:Myocytes, Cardiac D062085:RNA, Long Noncoding D020257:Ventricular Remodeling
myogenesis lncRNAs CRISPR/Cas9 epigenetic control heart development