Genome-wide association mapping of Sclerotinia sclerotiorum resistance in soybean using whole-genome resequencing data.
BACKGROUND
Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum (Lib.) de Bary, is an important cause of yield loss in soybean. Although many papers have reported different loci contributing to partial resistance, few of these were proved to reproduce the same phenotypic impact in different populations.


RESULTS
In this study, we identified a major quantitative trait loci (QTL) associated with resistance to SSR progression on the main stem by using a genome-wide association mapping (GWAM). A population of 127 soybean accessions was genotyped with 1.5 M SNPs derived from genotyping-by-sequencing (GBS) and whole-genome sequencing (WGS) ensuring an extensive genome coverage and phenotyped for SSR resistance. SNP-trait association led to discovery of a new QTL on chromosome 1 (Chr01) where resistant lines had shorter lesions on the stem by 29 mm. A single gene (Glyma.01 g048000) resided in the same LD block as the peak SNP, but it is of unknown function. The impact of this QTL was even more significant in the descendants of a cross between two lines carrying contrasted alleles for Chr01. Individuals carrying the resistance allele developed lesions almost 50% shorter than those bearing the sensitivity allele.


CONCLUSION
These results suggest that the new region on chromosome 1 harbors a promising resistance QTL to SSR that can be used in soybean breeding program.
['Boudhrioua|Chiheb|C|', 'Bastien|Maxime|M|', 'Torkamaneh|Davoud|D|', 'Belzile|François|F|http://orcid.org/0000-0001-6893-4529']
D001203:Ascomycota D002874:Chromosome Mapping D032461:Chromosomes, Plant D060467:Disease Resistance D055106:Genome-Wide Association Study D060005:Genotyping Techniques D010935:Plant Diseases D020641:Polymorphism, Single Nucleotide D040641:Quantitative Trait Loci D013025:Soybeans
Sclerotinia sclerotiorum Soybean GWAM WGS