Smilax china leaf extracts suppress pro-inflammatory adhesion response in human umbilical vein endothelial cells and proliferation of HeLa cells.
Objective: The objective of this study was to investigate the anti-inflammatory and anticancer effects of the leaves of Smilax china.Methodology: The aqueous extract was examined for its anti-inflammatory effects on tumour necrosis factor (TNF)-α-induced inflammation in HUVECs whereas the aqueous (water), ethyl acetate (EA), butanol (B) and methylene chloride (MC) extracts were examined for their anticancer effect on HeLa cells.Results: The aqueous extract suppressed the (TNF)-α-induced expression of ICAM-1, VCAM-1 and TNF-R1 and attenuated the expression of MCP-1, MMP-9, NF-kB and IFN-γ. The MC extract suppressed the proliferation of HeLa cells at all doses employed (50, 150, and 300 µg/ml). The EA extract demonstrated appreciable anti-proliferative effect whereas the BuOH extract demonstrated mild anti-proliferative activity. The aqueous extract did not show any significant anti-proliferative effect. None of the extracts were toxic to the normal cells (HUVECs).Conclusion: Smilax china leaf extracts possess significant anti-inflammatory and anticancer effects.
['Tettey|Clement O|CO|', 'Yang|Injun|I|', 'Shin|Heung-Mook|HM|']
D000893:Anti-Inflammatory Agents D000970:Antineoplastic Agents D002448:Cell Adhesion D049109:Cell Proliferation D006367:HeLa Cells D061307:Human Umbilical Vein Endothelial Cells D006801:Humans D007249:Inflammation D010936:Plant Extracts D018515:Plant Leaves D032281:Smilax
Smilax china Pro-inflammatory anticancer HUVECs endothelium