The P2X7 Receptor, Cathepsin S and Fractalkine in the Trigeminal Subnucleus Caudalis Signal Persistent Hypernociception in Temporomandibular Rat Joints.
Temporomandibular joint (TMJ) is frequently involved with rheumatoid arthritis with a high prevalence that could result in a chronic pain state. Once the disease is established in the joint, the antigen-specific immune reaction initiates a neuro-immune cascade of events that causes sensitization of the central nervous system. This study establishes animal experimental models that evaluate the chronicity of albumin-induced arthritis hypernociception in the TMJ. Antigen-induced arthritis was generated in rats with methylated bovine serum albumin (mBSA) diluted in complete Freund's. Intra-articular injection of mBSA (10 µg/TMJ/week) during 3 weeks resulted in a persistent inflammatory hypernociception which was characterized by an inflammatory episode characterized by the increased of lymphocytes, macrophages and pro-inflammatory interleukins IL-12 and IL-18. The persistent model of inflammatory hypernociception induced by arthritis in the TMJ elicited protein levels of P2X7 receptors, cathepsin S and fractalkine in the trigeminal subnucleus caudalis. Overall, the results of the present work suggest that a persistent inflammatory hypernociception of albumin-induced arthritis in the TMJ leads to the activation of the central nervous system signaling by P2X7/cathepsin S/fractalkine pathway.
['Bonfante|Ricardo|R|', 'Napimoga|Marcelo Henrique|MH|', 'Macedo|Cristina Gomes|CG|', 'Abdalla|Henrique Ballassini|HB|', 'Pieroni|Victor|V|', 'Clemente-Napimoga|Juliana Trindade|JT|']
D000818:Animals D001168:Arthritis D001169:Arthritis, Experimental D002403:Cathepsins D054428:Chemokine CX3CL1 D004195:Disease Models, Animal D008297:Male D059225:Nociception D017208:Rats, Wistar D058486:Receptors, Purinergic P2X7 D012710:Serum Albumin, Bovine D015398:Signal Transduction D013705:Temporomandibular Joint Disorders D014278:Trigeminal Nuclei
temporomandibular joint arthritis sensitization inflammation microglial cells persistent pain