Metatranscriptomic analysis of modified atmosphere packaged poultry meat enables prediction of Brochothrix thermosphacta and Carnobacterium divergens in situ metabolism.
In this study, in situ-expressed metabolic routes of Brochothrix (B.) thermosphacta and Carnobacterium (C.) divergens were evaluated based on a metatranscriptomic dataset from bacteria growing on MAP chicken meat (O2/CO2; N2/CO2). Both species exhibited no (C. divergens) or minor transcription regulation (B. thermosphacta) within their main metabolic routes in response to different atmospheres. Both employ pathways related to glucose and ribose. Gluconeogenesis from lipid-borne glycerol is active in the progressing lack of carbohydrates. Pyruvate fates in both species comprise lactate, ethanol, acetate, CO2, formate, C4-compounds and H2O2 (only B. thermosphacta). Both species express genes for a minimal aerobic respiratory chain, but do not possess the genetic setting for a functional citric acid cycle. While products of carbohydrate and glycerol metabolism display mild to medium sensorial off-characteristics, predicted end products of their amino acid metabolism comprise, e.g., isobutyrate and isovalerate (B. thermosphacta) or cadaverine and tyramine (C. divergens) as potent spoilage compounds.
['Höll|Linda|L|', 'Hilgarth|Maik|M|', 'Geissler|Andreas J|AJ|', 'Behr|Jürgen|J|', 'Vogel|Rudi F|RF|http://orcid.org/0000-0001-6873-5386']
D000818:Animals D001272:Atmosphere D058640:Brochothrix D056573:Carnobacterium D015169:Colony Count, Microbial D005516:Food Microbiology D018857:Food Packaging D006861:Hydrogen Peroxide D008460:Meat D011200:Poultry D059467:Transcriptome
Metatranscriptomics Modified atmosphere packaging Brochothrix thermosphacta Carnobacterium divergens Meat spoilage