Antioxidant and anti-inflammatory activities of Aerva pseudotomentosa leaves.
CONTEXT
Aerva pseudotomentosa Blatt. & Hallb. (Amaranthaceae), commonly called "Bui";, is a medicinal plant of the arid region. It is used for the treatment of inflammatory disorders, such as rheumatic pain, and healing of wounds, which are associated with oxidative stress.


OBJECTIVE
The present study evaluated the antioxidant potential of Aerva pseudotomentosa leaves by in vitro models and its anti-inflammatory effect in rats.


MATERIAL AND METHODS
The aqueous extract (APAE) was analyzed by HPTLC and HPLC. The antioxidant effect of APAE was evaluated by various in vitro methods [DPPH (1, 1-diphenyl-2-picryl-hydrazil) and hydrogen peroxide free radical scavenging, reducing power, and anti-lipid peroxidation assays]. Anti-inflammatory effect was studied in carrageenan and formalin-induced paw oedema models in rats. APAE (200 and 400 mg/kg) and standard drug, indomethacin (10 mg/kg), were administered orally 1 h before carrageenan/formalin administration and inflammation was noted up to 5 h.


RESULTS
HPLC analysis of APAE revealed the presence of rutin. APAE showed significant scavenging effect on DPPH (IC50 49.37 μ g/mL) and peroxide (IC50 288.2 μ g/mL) radicals. The extract exhibited reducing potential and inhibition of lipid peroxidation. APAE treatment significantly attenuated mean increase in paw volume and exhibited inhibition of paw oedema in both in vivo models with inhibition of 45.11% and 49.42%, respectively at 5 h.


DISCUSSION AND CONCLUSION
APAE exhibited <italic>in vitro</italic> antioxidant and anti-inflammatory activities. Anti-inflammatory effect of APAE may be attributed to its antioxidant potential, due to the presence of rutin and other phenolics. This study substantiates folk use of leaves in inflammatory disorders.
['Pandey|Abhishek|A|', 'Kaushik|Atul|A|', 'Wanjari|Manish|M|', 'Dey|Yadu Nandan|YN|', 'Jaiswal|Bhagat Singh|BS|', 'Dhodi|Anamika|A|']
D027703:Amaranthaceae D000818:Animals D000893:Anti-Inflammatory Agents D000975:Antioxidants D002851:Chromatography, High Pressure Liquid D004195:Disease Models, Animal D004305:Dose-Response Relationship, Drug D004487:Edema D005260:Female D007213:Indomethacin D007249:Inflammation D015227:Lipid Peroxidation D008297:Male D018384:Oxidative Stress D010636:Phenols D010936:Plant Extracts D018515:Plant Leaves D051381:Rats D017208:Rats, Wistar D012431:Rutin
Bui DPPH Carrageenan Rutin Desert zone Flavonoids