Surface functionalization of titanium substrates with Deoxyribonuclease I inhibit peri-implant bacterial infection.
This study aimed to investigate the effect of Deoxyribonuclease I (DNase I) coating on initial adhesion and biofilm formation of peri-implant bacteria. Titanium (Ti), Ti-polydopamine (Ti-PDOP), Ti-PDOP-DNase I and Ti-PDOP-inactivated DNase I samples were studied. The FE-SEM, EDS and XPS were used to confirm that DNase I was coated onto Ti. The initial adhesion and biofilm formation of Aggregatibacter actinomycetemcomitans (A.a) and Fusobacterium nucleatum (F.n) were observed by CLSM. The osteogenic induction of Ti-PDOP-DNase I on MC3T3-E1 cells was investigated by ALP activity and RT-PCR. The adhesion clearance rate of viable bacteria on the surfaces of Ti-PDOP-DNase I was 91.95% for A.a, and 96.37% for F.n, and the 24 h biofilm formation of the bacteria was significantly inhibited. In addition, on DNase I coating, the mRNA level of osteogenic marker genes (alp, opn, bsp, sp7) and the activity of ALP were both up-regulated. Therefore, DNase I coating could be an alternative approach for preventing implant-related infection.
['Shao|Can|C|', 'Zhang|Xin|X|', 'Ye|Jing|J|', 'Li|Ya-Chong|YC|', 'Bao|Yi-Jun|YJ|', 'Li|Zhi-Hui|ZH|', 'Huang|Ying|Y|', 'Liu|Ying|Y|']
D001422:Bacterial Adhesion D001424:Bacterial Infections D018441:Biofilms D003850:Deoxyribonuclease I D016967:Fusobacterium nucleatum D006801:Humans D011108:Polymers D013499:Surface Properties D014025:Titanium
Deoxyribonuclease I (DNase I) Adhesion Biofilm Titanium (Ti) Dental implant