Anti-glioma properties of DVL, a lectin purified from Dioclea violacea.
Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line. DVL treatment was able to induce caspase-3 activation, apoptotic cell death and cellular membrane damage. Furthermore, DVL decreased mitochondrial membrane potential and increased the number of acidic vesicles and cleavage of LC3, indicating activation of autophagic processes. DVL also significantly inhibited cell migration. Compared to ConA, a well-studied lectin extracted from Canavalia ensiformes seeds, some effects of DVL were more potent, including decreasing C6 glioma cell viability and migration ability. Taken together, the results suggest that DVL can induce glioma cell death, autophagy and inhibition of cell migration, displaying potential anti-glioma activity.
['Nascimento|Ana Paula M|APM|', 'Knaut|Jhônatas L|JL|', 'Rieger|Débora K|DK|', 'Wolin|Ingrid A V|IAV|', 'Heinrich|Isabella A|IA|', 'Mann|Josiane|J|', 'Juarez|Andrea V|AV|', 'Sosa|Liliana Del V|LDV|', 'De Paul|Ana Lucia|AL|', 'Moreira|Cleane G|CG|', 'Silva|Ivanice B|IB|', 'Nobre|Clareane S|CS|', 'Osterne|Vinicius J S|VJS|', 'Nascimento|Kyria S|KS|', 'Cavada|Benildo S|BS|', 'Leal|Rodrigo B|RB|']
D000818:Animals D017209:Apoptosis D001343:Autophagy D032449:Canavalia D053148:Caspase 3 D002453:Cell Cycle D045744:Cell Line, Tumor D002462:Cell Membrane D002465:Cell Movement D049109:Cell Proliferation D003208:Concanavalin A D052598:Dioclea D015870:Gene Expression D007770:L-Lactate Dehydrogenase D053078:Membrane Potential, Mitochondrial D008869:Microtubule-Associated Proteins D008928:Mitochondria D009457:Neuroglia D037121:Plant Lectins D051381:Rats
DVL Lectin Dioclea violacea Glioma ConA Autophagy