Muscadine Grape (Vitis rotundifolia) or Wine Phytochemicals Reduce Intestinal Inflammation in Mice with Dextran Sulfate Sodium-Induced Colitis.
The objective of this study was to determine the anti-inflammatory effects of phytochemical extracts from muscadine grapes or wine on dextran sulfate sodium (DSS)-induced colitis in mice and to investigate cellular mechanisms. Two groups of C57BL/6J mice were gavaged with muscadine grape phytochemicals (MGP) or muscadine wine phytochemicals (MWP), respectively, for 14 days. Acute colitis was induced by 3% DSS in drinking water for 7 days. An additional two groups of mice served as healthy and disease controls. Results indicated that MGP or MWP significantly prevented weight loss, reduced disease activity index, and preserved colonic length compared to the colitis group (p ≤ 0.05). MGP or MWP significantly decreased myeloperoxidase activity as well as the levels of IL-1β, IL-6, and TNF-α in colon (p ≤ 0.05). MGP or MWP caused down-regulation of the NF-κB pathway by inhibiting the phosphorylation and degradation of IκB in a dose-dependent manner. These findings suggest that phytochemicals from muscadine grape or wine mitigate ulcerative colitis via attenuation of pro-inflammatory cytokine production and modulation of the NF-κB pathway.
['Li|Ruiqi|R|http://orcid.org/0000-0001-8206-0362', 'Kim|Min-Hyun|MH|', 'Sandhu|Amandeep K|AK|', 'Gao|Chi|C|', 'Gu|Liwei|L|']
D000818:Animals D000893:Anti-Inflammatory Agents D003092:Colitis D003106:Colon D016264:Dextran Sulfate D004195:Disease Models, Animal D005260:Female D006801:Humans D020672:I-kappa B Proteins D053583:Interleukin-1beta D015850:Interleukin-6 D007422:Intestines D051379:Mice D008810:Mice, Inbred C57BL D016328:NF-kappa B D010936:Plant Extracts D027843:Vitis D014920:Wine
muscadine grape wine phytochemicals intestinal inflammation NF-κB