Overview of Analytical-to-Preparative Liquid Chromatography Method Development.
Purification of compounds is a necessary aspect of chemical synthesis. Developing an efficient purification method is time-consuming. A method that quickly calculates preparative gradients from analytical scouting runs is described. A solvent composition that provides the desired retention of a model compound is used to calibrate the analytical scouting run to determine an apparent gradient delay. This delay is applied to the retention times of compounds run with the same scouting gradient to yield a solvent composition for preparative purification.
['Silver|Jack|J|0000-0003-3724-639X']
D001688:Biological Products D002853:Chromatography, Liquid D057927:Hydrophobic and Hydrophilic Interactions D008956:Models, Chemical D009930:Organic Chemicals D011506:Proteins D012822:Silicon Dioxide D012997:Solvents D013997:Time Factors
liquid chromatography method development purification analytical chromatography focused gradient preparative chromatography scale up