Clinical significance of plasma MMP-2 and MMP-9 levels as biomarkers for tumor expression in breast cancer patients in Egypt.
Matrix metallopeptidase 2 (MMP-2) and matrix metallopeptidase 9 (MMP-9) are involved in the breakdown of extracellular matrix in normal physiological processes as well as in disease processes, such as cancer metastasis. We conducted this work to study the role of MMP-2 and MMP-9 in breast cancer by measuring their plasma concentrations before and after surgery. Also, to examine if their levels can reflect the stage of disease and prognosis. Forty-eight breast cancer patients and 13 patients with benign breast diseases were included in the study. MMP-2 and MMP-9 levels were measured by ELISA and semi-quantitative real-time PCR. MMP-2 and MMP-9 levels in plasma were determined by ELISA immediately before surgery and during 6 to 12 months after curative surgery. We observed a significant increase in the level of MMP-9 mRNA expression in breast cancer patients in comparison to their normal breast tissues and to tissues of benign breast disease. In all TNM tumor stages, the plasma levels of MMP-2 and MMP-9 were increased significantly before curative surgery in the studied patients with breast carcinoma and decreased significantly after surgery. Both MMP-2 and MMP-9 may be used as a possible marker for follow-up or as a marker that reflects the response of the disease to treatment.
['Alrehaili|Amani Ahmed|AA|', 'Gharib|Amal Fathi|AF|', 'Karam|Rehab Ahmed|RA|', 'Alhakami|Reem Ali|RA|', 'El Sawy|Wael Hassan|WH|', 'Abd Elrahman|Tamer Mohamed|TM|']
D000328:Adult D000368:Aged D014408:Biomarkers, Tumor D001943:Breast Neoplasms D004534:Egypt D004797:Enzyme-Linked Immunosorbent Assay D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D020778:Matrix Metalloproteinase 2 D020780:Matrix Metalloproteinase 9 D008875:Middle Aged D009367:Neoplasm Staging D059035:Perioperative Period D011379:Prognosis D055815:Young Adult
MMP-2 MMP-9 Breast cancer TNM staging