MiR-451a ameliorates alcoholic hepatitis via repressing HDAC8-mediated proinflammatory response.
Alcoholic hepatitis (AH) is identified as an inflammatory syndrome with high morbidity and mortality as a result of severe hepatocellular dysfunction and liver injury. Accumulated studies indicated that miRNAs are involved in AH. The potential effect of miR-451a in AH mice was examined in the current study. A mice AH model was established and the miR-451a expression in AH mice compared with the sham group was tested by real-time polymerase chain reaction (qRT-PCR). AH mice were injected with pre-miR-451a lentivirus for miR-451a overexpression and histone deacetylase (HDAC8) lentivirus for HDAC8 overexpression in AH mice. The underlying mechanisms were explored by searching the potential target genes of miR-451a in miRanda database and then we confirmed this. We found that miR-451a expression was significantly decreased in AH mice compared with the sham group. Moreover, miR-451a overexpression alleviated alcohol-induced liver inflammation and injuries of AH mice. Additionally, further mechanism exploration disclosed that HDAC8 was a target of miR-451a. The protective effect of miR-451a on AH in AH mice was abolished by HDAC8 overexpression. In summary, miR-451a ameliorates AH via repressing HDAC8-mediated proinflammatory response.
['Du|Bo|B|', 'Tan|Xiao-Hong|XH|', 'Cheng|Ling|L|', 'Wang|Feng|F|', 'Zhang|Hai-Feng|HF|https://orcid.org/0000-0002-3986-0193']
D000818:Animals D020029:Base Pairing D001483:Base Sequence D002460:Cell Line D049109:Cell Proliferation D030541:Databases, Genetic D004195:Disease Models, Animal D005786:Gene Expression Regulation D005822:Genetic Vectors D006519:Hepatitis, Alcoholic D022781:Hepatocytes D006655:Histone Deacetylases D006801:Humans D007249:Inflammation D016086:Lentivirus D008099:Liver D051379:Mice D008810:Mice, Inbred C57BL D035683:MicroRNAs D009843:Oligoribonucleotides D011725:Pyridines D015398:Signal Transduction
miR-451a alcoholic hepatitis HDAC8 inflammation