N-acetylcysteine inhibits Mancozeb-induced impairments to the normal development of zebrafish embryos.
Mancozeb (MZ), a manganese/zinc-containing ethylene-bis-dithiocarbamate (EBCD) fungicide has been claimed to present low acute toxicity and short environmental persistence, however, its effects on embryogenesis in non-target organisms is unclear. Here, we used zebrafish embryos (5 hpf) to assess the potential embryotoxic effects induced by MZ (up to 72 hpf) as well as the role of reactive oxygen species (ROS) in this process by pre-treatment with a classical antioxidant (N-acetylcysteine, NAC). Markers of reactive oxygen species production (ROS), glutathione (GSH) levels and glutathione S-transferase (GST) activity were measured along with genotoxicity (comet assay), cell death (Acridine Orange) and behavioral parameters (spontaneous movement, touch stimulation and swimming response), in order to determine potential mechanisms of embryotoxicity. According to results, MZ was able to induce morphological abnormalities such as body axis distortion, DNA damage, cell death, increased ROS generation and changes in behavioral endpoints during zebrafish development. All these toxic effects were inhibited by the pre-treatment with NAC indicating a key role of redox unbalance during MZ-induced embryotoxicity. At least in our knowledge, this is the first report on the deleterious effect of MZ to the normal embryogenesis of zebrafish. In addition, the importance of ROS generation during this pathophysiological condition was highlighted.
['Costa-Silva|Dennis Guilherme da|DGD|', 'Leandro|Luana Paganotto|LP|', 'Vieira|Pratícia de Brum|PB|', 'de Carvalho|Nelson Rodrigues|NR|', 'Lopes|Andressa Rubim|AR|', 'Schimith|Lucia Emanueli|LE|', 'Nunes|Mauro Eugênio Medina|MEM|', 'de Mello|Renata Siqueira|RS|', 'Martins|Illana Kemmerich|IK|', 'de Paula|Adriano Alves|AA|', 'Cañedo|Andrés Delgado|AD|', 'Moreira|José Cláudio Fonseca|JCF|', 'Posser|Thais|T|', 'Franco|Jeferson Luis|JL|']
D000111:Acetylcysteine D000818:Animals D001522:Behavior, Animal D016923:Cell Death D020552:Comet Assay D004249:DNA Damage D004625:Embryo, Nonmammalian D047108:Embryonic Development D005659:Fungicides, Industrial D008344:Maneb D017382:Reactive Oxygen Species D015027:Zebrafish D015038:Zineb
Fungicide Embryotoxicity Reactive oxygen species Antioxidant Dithiocarbamate