Injection of Porcine Adipose Tissue-Derived Stromal Cells by a Novel Waterjet Technology.
Previously, we developed a novel, needle-free waterjet (WJ) technology capable of injecting viable cells by visual guided cystoscopy in the urethral sphincter. In the present study, we aimed to investigate the effect of WJ technology on cell viability, surface markers, differentiation and attachment capabilities, and biomechanical features. Porcine adipose tissue-derived stromal cells (pADSCs) were isolated, expanded, and injected by WJ technology. Cell attachment assays were employed to investigate cell-matrix interactions. Cell surface molecules were analyzed by flow cytometry. Cells injected by Williams Needle (WN), normal cannula, or not injected cells served as controls. Biomechanical properties were assessed by atomic force microscopy (AFM). pADSCs injected by the WJ were viable (85.9%), proliferated well, and maintained their in vitro adipogenic and osteogenic differentiation capacities. The attachment of pADSCs was not affected by WJ injection and no major changes were noted for cell surface markers. AFM measurements yielded a significant reduction of cellular stiffness after WJ injections (p < 0.001). WJ cell delivery satisfies several key considerations required in a clinical context, including the fast, simple, and reproducible delivery of viable cells. However, the optimization of the WJ device may be necessary to further reduce the effects on the biomechanical properties of cells.
['Danalache|Marina|M|0000-0002-7217-2499', 'Knoll|Jasmin|J|', 'Linzenbold|Walter|W|', 'Enderle|Markus|M|', 'Abruzzese|Tanja|T|', 'Stenzl|Arnulf|A|0000-0003-4207-9928', 'Aicher|Wilhelm K|WK|0000-0003-2210-6773']
D050156:Adipogenesis D000273:Adipose Tissue D000818:Animals D002454:Cell Differentiation D049109:Cell Proliferation D002470:Cell Survival D006801:Humans D007267:Injections D045164:Mesenchymal Stem Cell Transplantation D059630:Mesenchymal Stem Cells D010012:Osteogenesis D017154:Stromal Cells D013552:Swine
stromal cells waterjet viability atomic force microscopy elasticity regeneration urinary incontinence