Dibutyl phthalate aggravated asthma-like symptoms through oxidative stress and increasing calcitonin gene-related peptide release.
Dibutyl phthalate (DBP) is one of the most ubiquitous phthalate esters found in everyday products, and is receiving increased attention as an immunologic adjuvant. However, information regarding DBP-aggravated allergic asthma is still limited. This study used a mouse model sensitized with ovalbumin (OVA) to determine any adverse effects of DBP on allergic asthma. Our results reveal that allergic asthmatic mice exposed to DBP for an extended period had a significant increase in inflammatory cell infiltration; a significant increase in levels of serum immunoglobulin and T helper 2 cell (Th2) and T helper 17 cell (Th17) cytokines in lung tissue; and significant changes in lung histology and AHR, all of which are typical asthmatic symptoms. The levels of oxidative stress and levels of the neuropeptide, calcitonin gene related peptide (CGRP), were also elevated after DBP exposure. Interestingly, blocking oxidative stress by administering melatonin (MT) not only reduced oxidative stress and CGRP levels, but also ameliorated the asthmatic symptoms. Collectively, these results show that DBP exacerbates asthma-like pathologies by increasing the expression of CGRP mediated by oxidative stress.
['Zhou|Sangyu|S|', 'Han|Man|M|', 'Ren|Yaolin|Y|', 'Yang|Xu|X|', 'Duan|Liju|L|', 'Zeng|Yan|Y|', 'Li|Jinquan|J|']
D000818:Animals D001249:Asthma D015740:Calcitonin Gene-Related Peptide D016207:Cytokines D003993:Dibutyl Phthalate D004195:Disease Models, Animal D004785:Environmental Pollutants D008168:Lung D008550:Melatonin D051379:Mice D008807:Mice, Inbred BALB C D010047:Ovalbumin D018384:Oxidative Stress D058504:Th17 Cells D018418:Th2 Cells
Dibutyl phthalate Oxidative stress Calcitonin gene-related peptide Allergic asthma Melatonin