Cell-based skin substitutes accelerate regeneration of extensive burn wounds in rats.
BACKGROUND
This study investigated the effects of amniotic membrane combined with adipose-derived stem cells or fetal fibroblasts on regenerating extensive burns in rats.


METHODS
Third degree burns of 1100-1800 mm2 were induced on 32 Sprague-Dawley rats. Burned sites were excised and randomly covered with Vaseline gauze (control), human amniotic membrane (HAM), human fetal fibroblasts seeded on HAM (HAM-FF), or human adipose-derived stem cells seeded on HAM (HAM-ASC), and followed by wound closure and histological assessments.


RESULTS
Wound closure rates of HAM-FF, HAM-ASC, HAM and control groups at seven and 14 days after the treatment were 42.2% and 81.9%, 41.9% and 81.7%, 33.5% and 74.2%, and 16.5% and 69.7%, respectively. Wounds of HAM-FF, HAM-ASC, HAM and control groups were closed on 40, 40, 50 and 60 days after the treatment, respectively (P < 0.05). Histological assessments revealed lower inflammatory cell infiltration in HAM-ASC and HAM-FF groups.


CONCLUSIONS
Cell-based engineered skin substitutes seem to accelerate wound regeneration, especially within the first 14 days.
['Motamed|Sadrollah|S|', 'Taghiabadi|Ehsan|E|', 'Molaei|Hojjat|H|', 'Sodeifi|Niloofar|N|', 'Hassanpour|Seyed Esmaeil|SE|', 'Shafieyan|Saeed|S|', 'Azargashb|Enzollah|E|', 'Farajzadeh-Vajari|Fatemeh|F|', 'Aghdami|Nasser|N|', 'Bajouri|Amir|A|']
D000273:Adipose Tissue D000650:Amnion D000818:Animals D002056:Burns D002478:Cells, Cultured D005347:Fibroblasts D006801:Humans D008297:Male D010577:Petrolatum D051381:Rats D017207:Rats, Sprague-Dawley D012038:Regeneration D013234:Stem Cells D023822:Tissue Engineering D014945:Wound Healing
Burn Stem cell Tissue engineering Wound healing