Molecular types of Cryptococcus gattii/Cryptococcus neoformans species complex from clinical and environmental sources in Nairobi, Kenya.
Cryptococcal meningitis infections cause high mortality rates among HIV-infected patients in Sub-Saharan Africa. The high incidences of cryptococcal infections may be attributed to common environmental sources which, if identified, could lead to institution of appropriate control strategies. To determine the genotypes of Cryptococcus gattii/C. neoformans- species complex from Nairobi, Kenya, 123 clinical and environmental isolates were characterised. Typing was done using orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphism (URA5-RFLP). The majority of the isolates [105/123; 85.4%] were C. neoformans genotype (AFLPI/VNI) and 1.6% AFLP1A/VNB/VNII, whereas (13%) were C. gattii (AFLP4/VGI). This is the first report on the genotypes of C. gattii/C. neoformans species complex from clinical and environmental sources in Nairobi, Kenya and the isolation of C. gattii genotype AFLP4/VGI from the environment in Kenya.
['Kangogo|Mourine|M|', 'Bader|Oliver|O|', 'Boga|Hamadi|H|', 'Wanyoike|Wanjiru|W|', 'Folba|Claudia|C|', 'Worasilchai|Navaporn|N|', 'Weig|Michael|M|', 'Groß|Uwe|U|', 'Bii|Christine C|CC|']
D000818:Animals D003453:Cryptococcosis D056285:Cryptococcus gattii D003455:Cryptococcus neoformans D004271:DNA, Fungal D004783:Environmental Microbiology D005838:Genotype D006801:Humans D007630:Kenya D016533:Mycological Typing Techniques D016133:Polymerase Chain Reaction D012150:Polymorphism, Restriction Fragment Length
molecular types Cryptococcus clinical Kenya environment