Green Tea Seed Oil Suppressed Aβ1⁻42-Induced Behavioral and Cognitive Deficit via the Aβ-Related Akt Pathway.
The aim of this study was to investigate the availability of seeds, one of the byproducts of green tea, and evaluate the physiological activity of seed oil. The ameliorating effect of green tea seed oil (GTO) was evaluated on H₂O₂-induced PC12 cells and amyloid beta (Aβ)1-42-induced ICR mice. GTO showed improvement of cell viability and reduced reactive oxygen species (ROS) production in H₂O₂-induced PC12 cells by conducting the 2',3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 2',7'-dichlorofluorescein diacetate (DCF-DA) analysis. Also, administration of GTO (50 and 100 mg/kg body weight) presented protective effects on behavioral and memory dysfunction by conducting Y-maze, passive avoidance, and Morris water maze tests in Aβ-induced ICR mice. GTO protected the antioxidant system by reducing malondialdehyde (MDA) levels, and by increasing superoxide dismutase (SOD) and reducing glutathione (GSH) contents. It significantly regulated the cholinergic system of acetylcholine (ACh) contents, acetylcholinesterase (AChE) activities, and AChE expression. Also, mitochondrial function was improved through the reduced production of ROS and damage of mitochondrial membrane potential (MMP) by regulating the Aβ-related c-Jun N-terminal kinase (JNK)/protein kinase B (Akt) and Akt/apoptosis pathways. This study suggested that GTO may have an ameliorating effect on cognitive dysfunction and neurotoxicity through various physiological activities.
['Kim|Jong Min|JM|', 'Park|Seon Kyeong|SK|', 'Kang|Jin Yong|JY|', 'Park|Su Bin|SB|', 'Yoo|Seul Ki|SK|', 'Han|Hye Ju|HJ|', 'Cho|Kyoung Hwan|KH|', 'Kim|Jong Cheol|JC|', 'Heo|Ho Jin|HJ|']
D016229:Amyloid beta-Peptides D000818:Animals D000975:Antioxidants D060825:Cognitive Dysfunction D006861:Hydrogen Peroxide D008813:Mice, Inbred ICR D018696:Neuroprotective Agents D018384:Oxidative Stress D016716:PC12 Cells D010446:Peptide Fragments D010938:Plant Oils D051057:Proto-Oncogene Proteins c-akt D051381:Rats D012639:Seeds D015398:Signal Transduction D013662:Tea
green tea seed oil, amyloid β, neuroprotective effect, Aβ-related Akt pathway