Infection of Helicobacter pylori and Atrophic Gastritis Influence Lactobacillus in Gut Microbiota in a Japanese Population.
Background
Suppression of gastric acid by proton pump inhibitors is associated with the increase of Lactobacillus in human gut microbiota. Gastric acid secretion is also suppressed by Helicobacter pylori infection and following atrophic gastritis. However, few studies have examined the association between H. pylori infection and Lactobacillus species in gut microbiota particularly in Japan.


Methods
A total of 1,123 adult subjects who participated in a health survey in Hirosaki City were studied. Infection of H. pylori was defined by both serum antibody and stool antigen test. The presence and the severity of atrophic gastritis were defined by the serum level of serum pepsinogens. Using 16S ribosomal RNA amplification from fecal samples, the relative abundance of Lactobacillus was calculated, and the composition ratio of each Lactobacillus species was surveyed.


Results
The relative abundance of the Lactobacillus in H. pylori-infected subjects with severe atrophic gastritis was higher comparing with those in subjects with mild atrophic gastritis and without atrophic gastritis (0.591 vs 0.068% and 0.033%, respectively; p < 0.001) and also that of non-infected subjects (0.033%; p < 0.001). In H. pylori non-infected subjects, both gender and age were not associated with the relative abundance of Lactobacillus in fecal samples. The proportion of Lactobacillus salivarius was high in H. pylori-infected subjects while that of Lactobacillus acidophilus was high in non-infected subjects.


Conclusion
Lactobacillus in human gut microbiota could be influenced by H. pylori infection and severity of atrophic gastritis in Japanese subjects.
['Iino|Chikara|C|', 'Shimoyama|Tadashi|T|', 'Chinda|Daisuke|D|', 'Arai|Tetsu|T|', 'Chiba|Daisuke|D|', 'Nakaji|Shigeyuki|S|', 'Fukuda|Shinsaku|S|']
D000328:Adult D000368:Aged D015415:Biomarkers D005260:Female D005757:Gastritis, Atrophic D000069196:Gastrointestinal Microbiome D016481:Helicobacter Infections D016480:Helicobacter pylori D006801:Humans D007564:Japan D007778:Lactobacillus D008297:Male D054892:Metagenome D056186:Metagenomics D008875:Middle Aged D062486:Public Health Surveillance D012336:RNA, Ribosomal, 16S
Helicobacter pylori atrophic gastritis Lactobacillus gut microbiota pepsinogen