PMab-52: Specific and Sensitive Monoclonal Antibody Against Cat Podoplanin for Immunohistochemistry.
Podoplanin (PDPN) is expressed in several normal tissues, such as lymphatic endothelial cells, podocytes of renal glomerulus, and type I alveolar cells of lung. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. Although monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, rabbit PDPN, dog PDPN, and bovine PDPN have been established, anticat PDPN (cPDPN) mAbs have not been developed. In this study, we immunized mice with Chinese hamster ovary (CHO)-K1 cell lines expressing cPDPN, and developed anti-cPDPN mAbs. One of the clones, PMab-52 (IgM, kappa), detected cPDPN specifically in flow cytometry and Western blot analysis. PMab-52 is also useful for detecting feline squamous cell carcinoma cells in immunohistochemical analysis. PMab-52 is expected to be useful for investigating the function of cPDPN in feline carcinomas.
['Yamada|Shinji|S|', 'Itai|Shunsuke|S|', 'Nakamura|Takuro|T|', 'Yanaka|Miyuki|M|', 'Saidoh|Noriko|N|', 'Chang|Yao-Wen|YW|', 'Handa|Saori|S|', 'Harada|Hiroyuki|H|', 'Kagawa|Yumiko|Y|', 'Ichii|Osamu|O|', 'Konnai|Satoru|S|', 'Kaneko|Mika K|MK|', 'Kato|Yukinari|Y|']
D000818:Animals D000911:Antibodies, Monoclonal D000918:Antibody Specificity D002294:Carcinoma, Squamous Cell D002415:Cats D002417:Cattle D004285:Dogs D000939:Epitopes D015870:Gene Expression D006801:Humans D007150:Immunohistochemistry D037181:Lectins, C-Type D008562:Membrane Glycoproteins D051379:Mice D010974:Platelet Aggregation D050199:Podocytes D011817:Rabbits D051381:Rats
monoclonal antibody cat podoplanin immunohistochemistry PDPN