Yfiler(®) Plus amplification kit validation and calculation of forensic parameters for two Austrian populations.
With the new 6-dye AmpFISTR(®) Yfiler(®) Plus amplification kit (Thermo Fisher Scientific, Waltham, MA, USA) a set of 25 Y-chromosomal short tandem repeat loci (Y-STRs), including seven rapidly mutating Y-STRs (RM Y-STRs), is now available for forensic DNA typing. In this study we present our validation data for the AmpFISTR(®) Yfiler(®) Plus amplification kit and show the results of Y-chromosomal typing of 425 unrelated male individuals from two Austrian populations (Salzburg and Upper Austria) with the AmpFISTR(®) Yfiler(®) Plus amplification kit. Forensic parameters were calculated and compared for four Y-STR marker sets. We also typed five brother pairs to evaluate the power of discrimination for related individuals. The AmpFISTR(®) Yfiler(®) Plus (Yfiler Plus) kit appeared to be unimpaired by typical inhibitors such as hematin and humic acid or by large amounts of female components. An upgrade of analyzed markers resulted in increased discrimination capacity that is crucial for forensic trace analysis.
['Pickrahn|Ines|I|', 'Müller|Eva|E|', 'Zahrer|Waltraud|W|', 'Dunkelmann|Bettina|B|', 'Cemper-Kiesslich|Jan|J|', 'Kreindl|Gabriele|G|', 'Neuhuber|Franz|F|']
D001317:Austria D041322:Chromosomes, Human, Y D016172:DNA Fingerprinting D053803:Forensic Genetics D005787:Gene Frequency D014644:Genetic Variation D005828:Genetics, Population D006239:Haplotypes D006801:Humans D008297:Male D018895:Microsatellite Repeats D016133:Polymerase Chain Reaction D011933:Reagent Kits, Diagnostic D015203:Reproducibility of Results
Yfiler Y-chromosome Y-STRs Rapidly mutating Y-STRs (RM Y-STRs) Yfiler Plus Haplotype diversity