Serum-derived hepatitis C virus 1a infection of human astrocyte cell line SVG.
Neuroinvasion of hepatitis C virus (HCV) is evidenced by recent clinical studies. In this study, serum-derived HCV infection of astrocytes was analysed. Astrocytes were infected with HCV-positive serum, and viral replication was assessed on different days postinfection. RT-PCR was positive for HCV-negative strand on 5th and 7th day postinfection in the HCV-positive serum-infected astrocytes. Real-time RNA count in the cell culture supernatant was steadily increasing from day 3 to day 7. To reconfirm the viral replication, astrocytes were treated with an antiviral before the serum infection, and the antiviral treatment significantly reduced the viral RNA count. Further, the virus-infected cells stained positive for the presence of viral core protein. Electron microscopy revealed the presence of HCV-like particles in the astrocyte cell culture supernatant. In conclusion, serum-derived HCV replicates in human astrocyte cell line SVG.
['Rajalakshmy|A R|AR|', 'Malathi|J|J|', 'Madhavan|H N|HN|']
D001253:Astrocytes D003470:Culture Media D005838:Genotype D016174:Hepacivirus D006526:Hepatitis C D006801:Humans D008854:Microscopy, Electron D012367:RNA, Viral D060888:Real-Time Polymerase Chain Reaction D044967:Serum D056189:Viral Tropism D014771:Virion D014776:Virus Cultivation D014779:Virus Replication
hepatitis C virus astrocytes viral replication immunofluorescence neuroinflammation polymerase chain reaction