Overexpression of the long noncoding RNA TRHDE-AS1 inhibits the progression of lung cancer via the miRNA-103/KLF4 axis.
AIM
The aim of this study was to explore the role and molecular basis of the long noncoding RNA (lncRNA) TRHDE-AS1 in lung cancer.


METHODS
We used real-time polymerase chain reaction to analyze the messenger RNA expression levels of TRHDE-AS1, miR-103, and KLF4. The cell viability, proliferation, and invasion rates were assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Cell Counting Kit-8, and Transwell assays to elucidate the role of TRHDE-AS1.


RESULTS
Our results demonstrated that the lncRNA TRHDE-AS1 is mainly located in the cytoplasm and that the cell proliferation and invasion were suppressed in the group of overexpressed TRHDE-AS1. We also showed that miR-103 could directly bind to TRHDE-AS1 and provided evidence of the oncogenic function of miR-103. Besides, we proved that miR-103 exerted its function by adjusting the expression level of the tumor-suppressor gene KLF4, and the expression level was negatively associated with miR-103.


CONCLUSION
In summary, we determined that the effects of TRHDE-AS1 on proliferation, invasion, and cell death could be rescued by the overexpression of miR-103. Our experiments demonstrate that the TRHDE-AS1/miR-103/KLF4 axis may provide new evidence for understanding the molecular basis of lung cancer.
['Zhuan|Bing|B|', 'Lu|Yuting|Y|', 'Chen|Qian|Q|', 'Zhao|Xia|X|', 'Li|Ping|P|', 'Yuan|Qun|Q|', 'Yang|Zhao|Z|0000-0002-8081-8253']
D000072283:A549 Cells D000818:Animals D017209:Apoptosis D002465:Cell Movement D049109:Cell Proliferation D018450:Disease Progression D005260:Female D015972:Gene Expression Regulation, Neoplastic D006801:Humans D000090062:Kruppel-Like Factor 4 D051741:Kruppel-Like Transcription Factors D008175:Lung Neoplasms D008297:Male D051379:Mice D035683:MicroRNAs D009361:Neoplasm Invasiveness D062085:RNA, Long Noncoding D015398:Signal Transduction D023041:Xenograft Model Antitumor Assays
TRHDE-AS1 lung cancer KLF4 miR-103