Fast and safe fabrication of a free-standing chitosan/alginate nanomembrane to promote stem cell delivery and wound healing.
Polymeric ultrathin membranes that are compatible with cells offer tremendous advantages for tissue engineering. In this article, we report a free-standing nanomembrane that was developed using a layer-by-layer self-assembly technique with a safe and sacrificial substrate method. After ionization, two oppositely charged polyelectrolytes, alginate and chitosan, were alternately deposited on a substrate of a solidified gelatin block to form an ultrathin nanomembrane. The space between the two adjacent layers was ∼200 nm. The thickness of the nanomembrane was proportional to the number of layers. The temperature-sensitive gelatin gel served as a sacrificial template at 37°C. The free-standing nanomembrane promoted bone marrow stem cell adhesion and proliferation. Fluorescence-activated cell sorting was used to analyze green-fluorescent-protein-positive mesenchymal stem cells from the wounds, which showed a significantly high survival and proliferation from the nanomembrane when cells were transplanted to mouse dorsal skin that had a full-thickness burn. The bone-marrow-stem-cell-loaded nanomembrane also accelerated wound contraction and epidermalization. Therefore, this methodology provides a fast and facile approach to construct free-standing ultrathin scaffolds for tissue engineering. The biocompatibility and free-standing nature of the fabricated nanomembrane may be particularly useful for stem cell delivery and wound healing.
['Kong|Yi|Y|', 'Xu|Rui|R|', 'Darabi|Mohammad Ali|MA|', 'Zhong|Wen|W|', 'Luo|Gaoxing|G|', 'Xing|Malcolm Mq|MM|', 'Wu|Jun|J|']
D000464:Alginates D000818:Animals D002056:Burns D048271:Chitosan D005260:Female D005434:Flow Cytometry D005780:Gelatin D020723:Glucuronic Acid D049452:Green Fluorescent Proteins D006603:Hexuronic Acids D008567:Membranes, Artificial D059630:Mesenchymal Stem Cells D008810:Mice, Inbred C57BL D049329:Nanostructures D033581:Stem Cell Transplantation D013234:Stem Cells D023822:Tissue Engineering D014945:Wound Healing
nanomembrane cell delivery wound healing layer-by-layer