Antibody Pull-Down Experiments in Fission Yeast.
Proteins act as executors for almost all kinds of cellular processes. The majority of proteins achieve their proper functions through interacting with other proteins. Knowing the binding partners of a protein is instrumental for understanding its function. The antibody pull-down method is a powerful and common approach to detect protein-protein interactions. Here, an antibody pull-down protocol is described for detecting protein-protein interactions in fission yeast Schizosaccharomyces pombe.
['Dong|Qianhua|Q|', 'Li|Fei|F|']
D000818:Animals D006801:Humans D047468:Immunoprecipitation D012568:Schizosaccharomyces D029702:Schizosaccharomyces pombe Proteins
Antibody Pull-down Fission yeast Protein-protein interaction Schizosaccharomyces pombe Co-immunoprecipitation In vivo