Hemicholinium-3 sensitive choline transport in human T lymphocytes: Evidence for use as a proxy for brain choline transporter (CHT) capacity.
The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. To advance research on the impact of CHT capacity in humans, we established the presence of the neuronal CHT protein in human T lymphocytes. Next, we demonstrated CHT-mediated choline transport in human T cells. To address the validity of T cell-based choline uptake as a proxy for brain CHT capacity, we isolated T cells from the spleen, and synaptosomes from cortex and striatum, of wild type and CHT-overexpressing mice (CHT-OXP). Choline uptake capacity in T cells from CHT-OXP mice was two-fold higher than in wild type mice, mirroring the impact of CHT over-expression on synaptosomal CHT-mediated choline uptake. Monitoring T lymphocyte CHT protein and activity may be useful for estimating human CNS cholinergic capacity and for testing hypotheses concerning the contribution of CHT and, more generally, ACh signaling in cognition, neuroinflammation and disease.
['Koshy Cherian|Ajeesh|A|', 'Parikh|Vinay|V|', 'Wu|Qi|Q|', 'Mao-Draayer|Yang|Y|', 'Wang|Qin|Q|', 'Blakely|Randy D|RD|', 'Sarter|Martin|M|']
D000818:Animals D001692:Biological Transport D001921:Brain D002794:Choline D004305:Dose-Response Relationship, Drug D005260:Female D006426:Hemicholinium 3 D006801:Humans D007963:Leukocytes, Mononuclear D008297:Male D026901:Membrane Transport Proteins D051379:Mice D008810:Mice, Inbred C57BL D008822:Mice, Transgenic D013601:T-Lymphocytes
Choline transporter T lymphocytes Acetylcholine Synaptosomes Genetic CHT variants