Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM).
Mass spectrometry-based phosphoproteomics is an indispensible technique used in the discovery and quantification of phosphorylation events on proteins in biological samples. The application of this technique to tissue samples is especially useful for the discovery of biomarkers as well as biological studies. We herein describe the application of a large-scale phosphoproteome analysis and SRM/MRM-based quantitation to develop a strategy for the systematic discovery and validation of biomarkers using tissue samples.
['Narumi|Ryohei|R|', 'Tomonaga|Takeshi|T|']
D000818:Animals D015415:Biomarkers D002846:Chromatography, Affinity D002851:Chromatography, High Pressure Liquid D002852:Chromatography, Ion Exchange D006801:Humans D007553:Isotope Labeling D013058:Mass Spectrometry D010449:Peptide Mapping D010750:Phosphoproteins D010766:Phosphorylation D011494:Protein Kinases D011499:Protein Processing, Post-Translational D040901:Proteomics D013379:Substrate Specificity D057188:Workflow
iTRAQ SRM MRM Phosphoproteome IMAC