Comparative Proteomic Profiling of Methicillin-Susceptible and Resistant Staphylococcus aureus.
Staphylococcus aureus is a highly successful human pathogen responsible for a wide range of infections. This study provides insights into the virulence, pathogenicity, and antimicrobial resistance determinants of methicillin-susceptible and methicillin-resistant S. aureus (MSSA; MRSA) recovered from non-healthcare environments. Three environmental MSSA and three environmental MRSA are selected for proteomic profiling using isobaric tag for relative and absolute quantitation tandem mass spectrometry (iTRAQ MS/MS). Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway annotation are applied to interpret the functions of the proteins detected. 792 proteins are identified in MSSA and MRSA. Comparative analysis of MRSA and MSSA reveals that 8 of out 792 proteins are upregulated and 156 are downregulated. Proteins that have differences in abundance are predominantly involved in catalytic and binding activity. Among 164 differently abundant proteins, 29 are involved in pathogenesis, antimicrobial resistance, stress response, mismatch repair, and cell wall synthesis. Twenty-two proteins associated with pathogenicity including SPA, SBI, CLFA, and DLT are upregulated in MRSA. Moreover, the upregulated pathogenic protein ENTC2 in MSSA is determined to be a super antigen, potentially capable of triggering toxic shock syndrome in the host. Enhanced pathogenicity, antimicrobial resistance, and stress response are observed in MRSA compared to MSSA.
['Xu|Zhen|Z|', 'Chen|Jiazhen|J|', 'Vougas|Kostas|K|', 'Shah|Ajit|A|', 'Shah|Haroun|H|', 'Misra|Raju|R|', 'Mkrtchyan|Hermine V|HV|0000-0001-7339-7297']
D000900:Anti-Bacterial Agents D008712:Methicillin D055624:Methicillin-Resistant Staphylococcus aureus D008826:Microbial Sensitivity Tests D040901:Proteomics D013211:Staphylococcus aureus D053719:Tandem Mass Spectrometry
comparative proteomics methicillin-resistant Staphylococcus aureus methicillin-susceptible Staphylococcus aureus