Single Cell Densitometry and Weightlessness Culture of Mesenchymal Stem Cells Using Magnetic Levitation.
Magnetic levitation methodology enables density-based separation of microparticles/cells and sustains cell culture in different media. Levitation process can be accomplished via negative magnetophoresis (diamagnetophoresis), where the applied magnetic force compensates gravitational acceleration and the density of the diamagnetic object (e.g., cell) determines its levitation height. Here we describe a portable, sensitive, and cost-effective technology that uses the principles of magnetic levitation to measure single cell density and cell culture under desired conditions.
['Anil-Inevi|Muge|M|', 'Yilmaz|Esra|E|', 'Sarigil|Oyku|O|', 'Tekin|H Cumhur|HC|', 'Ozcivici|Engin|E|']
D000818:Animals D002138:Calibration D002452:Cell Count D018929:Cell Culture Techniques D002478:Cells, Cultured D003720:Densitometry D007091:Image Processing, Computer-Assisted D008280:Magnetics D059630:Mesenchymal Stem Cells D051379:Mice D044085:Microfluidics D059010:Single-Cell Analysis D014893:Weightlessness
Stem cells Magnetic levitation Single cell density Real-time monitoring Simulated microgravity