Shiga toxin 2eB-transgenic lettuce vaccine is effective in protecting weaned piglets from edema disease caused by Shiga toxin-producing Escherichia coli infection.
Porcine edema disease (ED) is a toxemia that is caused by enteric infection with Shiga toxin 2e (Stx2e)-producing Escherichia coli (STEC) and is associated with high mortality. Since ED occurs most frequently during the weaning period, preweaning vaccination of newborn piglets is required. We developed stx2eB-transgenic lettuce as an oral vaccine candidate against ED and examined its protective efficacy using a piglet STEC infection model. Two serially developed Stx2eB-lettuce strains, 2BN containing ingredient Stx2eB constituting a concentration level of 0.53 mg Stx2eB/g of powdered lettuce dry weight (DW) and 2BH containing ingredient Stx2eB constituting a concentration level of 2.3 mg of Stx2eB/g of powdered lettuce DW, were evaluated in three sequential experiments. Taken the results together, oral administration of Stx2eB-lettuce vaccine was suggested to relieve the pathogenic symptoms of ED in piglets challenged with virulent STEC strain. Our data suggested that Stx2eB-lettuce is a promising first oral vaccine candidate against ED.
['Hamabata|Takashi|T|https://orcid.org/0000-0003-0429-6550', 'Sato|Toshio|T|', 'Takita|Eiji|E|https://orcid.org/0000-0002-4794-4899', 'Matsui|Takeshi|T|https://orcid.org/0000-0001-7541-4863', 'Imaoka|Taishi|T|', 'Nakanishi|Nobuo|N|', 'Nakayama|Keizo|K|', 'Tsukahara|Takamitsu|T|https://orcid.org/0000-0002-3902-5956', 'Sawada|Kazutoshi|K|https://orcid.org/0000-0003-0286-5035']
D000284:Administration, Oral D000818:Animals D000831:Animals, Newborn D001428:Bacterial Vaccines D004488:Edema Disease of Swine D004927:Escherichia coli Infections D018545:Lettuce D022641:Shiga Toxin 2 D054323:Shiga-Toxigenic Escherichia coli D013552:Swine D014774:Virulence D014886:Weaning
porcine edema disease Shiga toxin 2e-producing Escherichia coli stx2eB-transgenic lettuce vaccine