Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes.
The elucidation of biological processes involving reactive oxygen species (ROS) facilitates a better understanding of the underlying progression of non-communicable diseases. Fluorescent probes are a powerful tool to study various ROS and have the potential to become essential diagnostic tools. We have developed a series of coumarin fluorescent probes for the selective and sensitive detection of peroxynitrite (ONOO-), a key ROS. Coumarin based probes exhibit good photostability, large Stokes shift and high quantum yields. The three ratiometric probes all contain a boronate ester motif for the detection of ONOO- and a distinctive organelle targeting group. The study of ONOO- generation in a particular organelle will allow more precise disease profiling. Hence, targeting groups for the mitochondria, lysosome and endoplasmic reticulum were introduced into a coumarin scaffold. The three ratiometric probes displayed sensitive and selective detection of ONOO- over other ROS species. All three coumarin probes were evaluated in murine RAW264.7 macrophages for detection of basal and stimulated ONOO- formation.
['Weber|Maria|M|', 'Yamada|Namiko|N|', 'Tian|Xue|X|', 'Bull|Steven D|SD|', 'Minoshima|Masafumi|M|', 'Kikuchi|Kazuya|K|', 'Mackenzie|Amanda B|AB|', 'James|Tony D|TD|']
peroxynitrite fluorescence reactive oxygen species inflammation molecular probe