Evaluation of the expression level of 12/15 lipoxygenase and the related inflammatory factors (CCL5, CCL3) in respiratory syncytial virus infection in mice model.
Human respiratory syncytial virus (RSV) is a leading cause of acute respiratory infection during early childhood and imposes a great burden on patients, parents, and society. Disease is thought to be caused, at least partially, by an excessive immune response. Pulmonary leukocyte infiltration is the result of a coordinated expression of diverse chemokines with distinct cellular specificities. Lipoxygenases (LOXs), as a key enzyme catalyzing deoxygenation of poly unsaturated fatty acids, regulate inflammation and have been suggested to play an important role in the immune response in viral infection. To expand our understanding on the possible role of LOX in respiratory viral infection, we studied the 12/15- lipoxygenase expression in RSV-related airway inflammation, and the related inflammatory chemokines, Chemokine (C-C motif) ligand 5 (CCL5) and Chemokine (C-C motif) ligand 3(CC L3) in both lung tissue and Bronchoalveolar lavage (BAL) fluid during experimental RSV infection. RSV infection induced mRNA expression of CCL5 and CCL3 in both BAL and lung tissue cells. In addition RSV infection enhanced expression of 12/15-LOX in both BAL and lung cells. In conclusion, we confirm that RSV infection leads to the increased expression of 12/15 LOX and the related chemokines CCL5 and CCL3 in BAL fluid and lung tissue cells suggesting that the 12/15 LOX pathway could serve as a candidate target for prevention and treatment of RSV infection.
['Salimi|Vahid|V|', 'Ramezani|Ali|A|', 'Mirzaei|Habibollah|H|', 'Tahamtan|Alireza|A|', 'Faghihloo|Ebrahim|E|', 'Rezaei|Farhad|F|', 'Naseri|Maryam|M|', 'Bont|Louis|L|', 'Mokhtari-Azad|Talat|T|', 'Tavakoli-Yaraki|Masoumeh|M|']
D000818:Animals D001092:Arachidonate 12-Lipoxygenase D001093:Arachidonate 15-Lipoxygenase D001992:Bronchoalveolar Lavage Fluid D002460:Cell Line D054405:Chemokine CCL3 D018946:Chemokine CCL5 D018925:Chemokines D004195:Disease Models, Animal D005260:Female D006801:Humans D007249:Inflammation D008168:Lung D051379:Mice D008807:Mice, Inbred BALB C D012333:RNA, Messenger D018357:Respiratory Syncytial Virus Infections D012136:Respiratory Syncytial Viruses
12/15 lipoxygenases Respiratory syncytial virus Chemokine Inflammation