Recombinant Purification of the Periplasmic Portion of the LINC Complex.
Recombinant expression of proteins and their complexes is the routine laboratory procedure to generate pure reagents for biochemical and structural studies. Here we present the standard procedure developed in our lab for the production of milligram quantities of stoichiometric SUN-KASH complexes. The protocol was specifically developed for the purification of the periplasmic portion of LINC complexes.
['Cruz|Victor E|VE|', 'Schwartz|Thomas U|TU|']
D002846:Chromatography, Affinity D006801:Humans D046912:Multiprotein Complexes D009687:Nuclear Proteins D033903:Periplasmic Proteins D059748:Proteolysis D011994:Recombinant Proteins
LINC complex Co-expression Protein purification