Targeted Phosphoproteome Analysis Using Selected/Multiple Reaction Monitoring (SRM/MRM).
Mass spectrometry-based phosphoproteomics has been rapidly spread based on the advancement of mass spectrometry and development of efficient enrichment techniques for phosphorylated proteins or peptides. Non-targeted approach has been employed in most of the studies for phosphoproteome analysis. However, targeted approach using selected/multiple reaction monitoring (SRM/MRM) is an indispensible technique used for the quantitation of known targets especially when we have many samples to quantitate phosphorylation events on proteins in biological or clinical samples. We herein describe the application of a large-scale phosphoproteome analysis and SRM-based quantitation for the systematic discovery and validation of biomarkers.
['Adachi|Jun|J|', 'Narumi|Ryohei|R|', 'Tomonaga|Takeshi|T|']
D000818:Animals D015415:Biomarkers D002853:Chromatography, Liquid D006801:Humans D010455:Peptides D010750:Phosphoproteins D020543:Proteome D040901:Proteomics D053719:Tandem Mass Spectrometry
Phosphoproteomics Selected/multiple reaction monitoring Biomarker IMAC Targeted proteomics