CD200 has an important role in the differential diagnosis of mature B-cell neoplasms by multiparameter flow cytometry.
BACKGROUND
Multiparameter flow cytometry is a useful tool for the diagnostic evaluation of mature B-cell neoplasms (MBN). Recently, it has been shown that CD200 may improve the distinction between chronic lymphocytic leukemia (CLL; CD200+) and mantle cell lymphoma (MCL; CD200-), but the role of CD200 expression in atypical CLL and other MBN remains to be established.


METHODS
To address this issue, we investigated the expression of CD200 in 159 consecutive cases of MBN.


RESULTS
CD200 was strongly expressed in CLL and was revealed to be an excellent marker to distinguish CLL from MCL, even in cases of atypical CLL. However, lack of CD200 was not an exclusive finding of MCL, being also observed in other MBNs. Furthermore, CD200 was highly expressed in hairy cell leukemia, being useful in the differential diagnosis of lymphomas with villous lymphocytes. Herein, we propose an algorithm to classify CD5+ MBNs based on the expression of CD200, CD11c, heavy chain immunoglobulins, and Matutes score.


CONCLUSIONS
These results expand the understanding of the CD200 expression in MBNs, giving further support for the inclusion of this marker in the routine investigation by flow cytometry.
['Sandes|Alex F|AF|', 'de Lourdes Chauffaille|Maria|M|', 'Oliveira|Cláudia Regina M C|CR|', 'Maekawa|Yumi|Y|', 'Tamashiro|Nair|N|', 'Takao|Tânia T|TT|', 'Ritter|Estela C|EC|', 'Rizzatti|Edgar G|EG|']
D000328:Adult D000368:Aged D000369:Aged, 80 and over D015703:Antigens, CD D003937:Diagnosis, Differential D005260:Female D005434:Flow Cytometry D006801:Humans D016130:Immunophenotyping D015451:Leukemia, Lymphocytic, Chronic, B-Cell D020522:Lymphoma, Mantle-Cell D008297:Male D008875:Middle Aged
CD200 flow cytometry chronic lymphocytic leukemia mantle cell lymphoma lymphoma