Conductive hydrogel composed of 1,3,5-benzenetricarboxylic acid and Fe3+ used as enhanced electrochemical immunosensing substrate for tumor biomarker.
In this work, a new conductive hydrogel was prepared by a simple cross-linking coordination method using 1,3,5-benzenetricarboxylic acid as the ligand and Fe3+ as the metal ion. The hydrogel film was formed on a glassy carbon electrode (GCE) by a drop coating method, which can dramatically facilitate the transport of electrons. A sensitive label-free electrochemical immunosensor was fabricated following electrodeposition of gold nanoparticles (AuNPs) on a hydrogel film and immobilization of an antibody. Neuron-specific enolase (NSE), a lung cancer biomarker, was used as the model analyte to be detected. The proposed immunosensor exhibited a wide linear detection range of 1pgmL-1 to 200ngmL-1 and a limit of detection of 0.26pgmL-1 (the ratio of signal to noise (S/N)=3). Moreover, the detection of NSE in human serum samples showed satisfactory accuracy compared with the data determined by enzyme-linked immunosorbent assay (ELISA), indicating good analytical performance of the immunoassay.
['Wang|Huiqiang|H|', 'Han|Hongliang|H|', 'Ma|Zhanfang|Z|']
D014408:Biomarkers, Tumor D015374:Biosensing Techniques D004553:Electric Conductivity D004563:Electrochemistry D006046:Gold D006801:Humans D020136:Hydrogel, Polyethylene Glycol Dimethacrylate D007118:Immunoassay D057230:Limit of Detection D053768:Metal Nanoparticles D010751:Phosphopyruvate Hydratase D014233:Tricarboxylic Acids
Conductive hydrogel 1,3,5-Benzenetricarboxylic acid Tumor biomarker Electrochemical immunosensor Neuron-specific enolase Iron ion