Somatic Cell Nuclear Transfer in Mice: Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status.
Somatic cell nuclear transfer (SCNT) enables the production of animals from single cell nuclei. Unlike normally fertilized embryos, SCNT-derived embryos ectopically express the Xist gene from the maternal allele, because of the lack of Xist-repressing imprints in the somatic donor genome. This has severely compromised the development of SCNT-derived embryos, at least in mice. Here, we describe the basic protocol of mouse SCNT as well as a Xist knockdown SCNT procedure, which remarkably increases the efficiency of cloning mice.
['Inoue|Kimiko|K|', 'Matoba|Shogo|S|', 'Ogura|Atsuo|A|']
D000818:Animals D019976:Cloning, Organism D057890:Epigenomics D005260:Female D055785:Gene Knockdown Techniques D051379:Mice D053652:Nuclear Transfer Techniques D062085:RNA, Long Noncoding D049951:X Chromosome Inactivation
Xist Development Oocyte Preimplantation embryo