Comparative Transcriptomic Analysis of the Hematopoietic System between Human and Mouse by Single Cell RNA Sequencing.
(1) Background: mouse models are fundamental to the study of hematopoiesis, but comparisons between mouse and human in single cells have been limited in depth. (2) Methods: we constructed a single-cell resolution transcriptomic atlas of hematopoietic stem and progenitor cells (HSPCs) of human and mouse, from a total of 32,805 single cells. We used Monocle to examine the trajectories of hematopoietic differentiation, and SCENIC to analyze gene networks underlying hematopoiesis. (3) Results: After alignment with Seurat 2, the cells of mouse and human could be separated by same cell type categories. Cells were grouped into 17 subpopulations; cluster-specific genes were species-conserved and shared functional themes. The clustering dendrogram indicated that cell types were highly conserved between human and mouse. A visualization of the Monocle results provided an intuitive representation of HSPC differentiation to three dominant branches (Erythroid/megakaryocytic, Myeloid, and Lymphoid), derived directly from the hematopoietic stem cell and the long-term hematopoietic stem cells in both human and mouse. Gene regulation was similarly conserved, reflected by comparable transcriptional factors and regulatory sequence motifs in subpopulations of cells. (4) Conclusions: our analysis has confirmed evolutionary conservation in the hematopoietic systems of mouse and human, extending to cell types, gene expression and regulatory elements.
['Gao|Shouguo|S|', 'Wu|Zhijie|Z|', 'Kannan|Jeerthi|J|', 'Mathews|Liza|L|0000-0002-9977-7631', 'Feng|Xingmin|X|0000-0001-8018-2366', 'Kajigaya|Sachiko|S|', 'Young|Neal S|NS|']
D000818:Animals D019070:Cell Lineage D019143:Evolution, Molecular D005786:Gene Expression Regulation D006410:Hematopoiesis D006412:Hematopoietic Stem Cells D006801:Humans D051379:Mice D059010:Single-Cell Analysis D059467:Transcriptome
hematopoiesis cross-species analysis gene regulatory network single-cell RNA sequencing