Molecular epidemiology of rotavirus in four provinces of Angola before vaccine introduction.
Angola is a sub-Saharan country in southern Africa highly affected by diarrhoeal disease with limited epidemiological data regarding etiologic agents. This study was performed during 2012-2013, prior to rotavirus vaccine introduction, with the objective to detect and characterize the rotavirus strains circulating in four provinces of the country: Huambo, Luanda, Zaire, and Cabinda. A high rotavirus detection rate (35%, 117/334) was observed. G1 was the most common G-genotype (83.6%), whereas P[8] (50.9%) followed by P[6] (38.8%) were the most common P-types. G1P[8] was identified as the predominant combination (50%), followed by the unusual G1P[6] (29.3%). Strains such G2P[4], G8P[6], G9P[6], and G12P[6] were also found in lower frequencies (5.2-1.7%). The P[6] strains did not cluster in the phylogenetic trees according to their geographic origin or even the corresponding G-genotype, suggesting a limited number of recent introductions and extensive reassortment events. Our results represent the first report on rotavirus genotype profiles in Angola, showing a wide circulation of the unusual genotype G1P[6], and underline the importance of RV surveillance after the vaccine introduction. J. Med. Virol. 88:1511-1520, 2016. © 2016 Wiley Periodicals, Inc.
['Esteves|Aida|A|', 'Nordgren|Johan|J|', 'Pereira|Joana|J|', 'Fortes|Filomeno|F|', 'Dimbu|Rafael|R|', 'Saraiva|Nilton|N|', 'Mendes|Cristina|C|', 'Istrate|Claudia|C|']
D000352:Africa, Northern D000353:Africa, Southern D000810:Angola D000956:Antigens, Viral D036022:Capsid Proteins D002675:Child, Preschool D015023:Democratic Republic of the Congo D003967:Diarrhea D005243:Feces D005260:Female D005759:Gastroenteritis D005838:Genotype D006801:Humans D008297:Male D017720:Molecular Epidemiology D012367:RNA, Viral D012401:Rotavirus D012400:Rotavirus Infections D022243:Rotavirus Vaccines
diarrhoeal disease genotypes children