Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells.
This study isolated and characterized a novel polysaccharide (PNPS-0.3) from the residue of Panax notoginseng by gradient elution. PNPS-0.3 mainly consisted of a backbone of →4)- α-D-GalAp-(1 → 4-β-L-Rhap-1 → 4)-β-D-Galp-(1 → residues, with an α-L-Araf-1 → 5)-α-L-Araf-(1 → branch connecting to the backbone at O-3 of →4-β-L-Rhap-1 → and a molecular weight of 76,655 Da. Furthermore, the adjuvant potential of PNPS-0.3 with bone marrow dendritic cells (BMDCs) was investigated. The results suggested that PNPS-0.3 could induce maturation of BMDCs by reshaping the morphology, upregulating the CD40, CD80, CD86 and MHC II membrane phenotypic markers, and by promoting the secretion of TNF-α and IL-12 proinflammatory cytokines. Moreover, PNPS-0.3 can trigger the DC-induced T-cell immune response, as indicated by the higher expressions of CD4, CD8, CD69, and MHC II in T cells with increased secretion of INF-β. Furthermore, PNPS-0.3 can bind to the pattern recognition receptors (PRR) of Toll-like receptor 4 (TLR 4), Toll-like receptor 2 (TLR 2), and mannose receptor (MR) on BMDCs. PNPS-0.3 also upregulated the expressions of Myd88, IKKβ, PP65, T-P65, and NF-κB, suggesting that the TLR4/TLR2-NF-κB signaling pathway was involved in the immunomodulatory mechanism. In conclusion, the immunoadjuvant potential of novel PNPS-0.3 was characterized, which is beneficial for the future utilization and development of P. notoginseng.
['Liu|Shengnan|S|', 'Yang|Ye|Y|', 'Qu|Yuan|Y|', 'Guo|Xiaoxi|X|', 'Yang|Xiaoyan|X|', 'Cui|Xiuming|X|', 'Wang|Chengxiao|C|']
D000818:Animals D001853:Bone Marrow D001854:Bone Marrow Cells D018920:Coculture Techniques D016207:Cytokines D003713:Dendritic Cells D007155:Immunologic Factors D008297:Male D051379:Mice D008807:Mice, Inbred BALB C D016328:NF-kappa B D053723:Panax notoginseng D011134:Polysaccharides D015398:Signal Transduction D013601:T-Lymphocytes
Structural characterization Polysaccharide Bone marrow dendritic cells Immunomodulatory adjuvant Panax notoginseng (Burk.) F. H. Chen