Development and Characterization of Cell-Specific Androgen Receptor Knockout Mice.
Conditional gene targeting has revolutionized molecular genetic analysis of nuclear receptor proteins, however development and analysis of such conditional knockouts is far from simple, with many caveats and pitfalls waiting to snare the novice or unprepared. In this chapter, we describe our experience of generating and analyzing mouse models with conditional ablation of the androgen receptor (AR) from tissues of the reproductive system and other organs. The guidance, suggestions, and protocols outlined in the chapter provide the key starting point for analyses of conditional-ARKO mice, completing them as described provides an excellent framework for further focussed project-specific analyses, and applies equally well to analysis of reproductive tissues from any mouse model generated through conditional gene targeting.
["O'Hara|Laura|L|", 'Smith|Lee B|LB|']
D000818:Animals D018390:Gene Targeting D017930:Genes, Reporter D005838:Genotype D006728:Hormones D007124:Immunoenzyme Techniques D019426:Integrases D008297:Male D051379:Mice D018345:Mice, Knockout D008856:Microscopy, Fluorescence D060888:Real-Time Polymerase Chain Reaction D011944:Receptors, Androgen D013737:Testis D019076:Transgenes
Androgen receptor Conditional knockout Mouse ARKO Cre/lox Transgenic