Phenylobacterium deserti sp. nov., isolated from desert soil.
A novel bacterial strain, designated YIM 73061T, was isolated from the Cholistan desert in Punjab, Pakistan, and characterized by using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Phenylobacterium conjunctum FWC21T (97.6 %), Phenylobacterium lituiforme FaiI3T (97.4 %), Phenylobacteriumcomposti 4T-6T (97.0 %) and Phenylobacterium aquaticum W2-3-4T (96.8 %). Cells were Gram-stain-negative, aerobic and motile rods that formed orange colonies. The strain was oxidase- and catalase-positive. Growth occurred at 20-40 °C (optimum, 30-37 °C) at pH 5.0-8.0 (optimum, pH 7.0) and with 0-1 % (w/v) NaCl (optimum, 0-0.5 %). The major cellular fatty acids (>10 %) were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The polar lipid profile consisted of phosphatidylglycerol and four unidentified glycolipids. The major isoprenoid quinone was ubiquinone-10 (Q-10). The G+C content of the genomic DNA was 66.8 mol%. Strain YIM 73061T showed low levels of DNA-DNA relatedness to P. conjunctum FWC21T (27.2±2.6 %), P. lituiforme FaiI3T (24.6±1.1 %) and P.composti 4T-6T (18.4±3.1 %). On the basis of phylogenetic inference, chemotaxonomic characteristics and phenotypic data, strain YIM 73061T should be classified as representing a novel species, for which the name Phenylobacterium deserti sp. nov. is proposed. The type strain is YIM 73061T (=DSM 103871T=CCTCC AB 2016297T).
['Khan|Inam Ullah|IU|', 'Hussain|Firasat|F|', 'Habib|Neeli|N|', 'Wadaan|Mohammed A M|MAM|', 'Ahmed|Iftikhar|I|', 'Im|Wan-Taek|WT|', 'Hozzein|Wael N|WN|', 'Zhi|Xiao-Yang|XY|', 'Li|Wen-Jun|WJ|']
D015373:Bacterial Typing Techniques D001482:Base Composition D042063:Caulobacteraceae D004269:DNA, Bacterial D003889:Desert Climate D005227:Fatty Acids D006017:Glycolipids D009693:Nucleic Acid Hybridization D010154:Pakistan D010743:Phospholipids D010802:Phylogeny D012336:RNA, Ribosomal, 16S D017422:Sequence Analysis, DNA D012988:Soil Microbiology D014451:Ubiquinone
Phenylobacterium deserti sp. nov. Cholistan desert Pakistan polyphasic taxonomy