Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel.
BACKGROUND
Poly (ethylene glycol)-poly (ɛ-caprolactone)-poly (ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel has been demonstrated to be biocompatible and thermosensitive. In this study, its potential efficacy and mechanisms of preventing postsurgical abdominal adhesions were investigated.


RESULTS
PECE hydrogel was transformed into gel state from sol state in less than 20 seconds at 37°C. None of the animals treated with the hydrogel (n = 15) developed adhesions. In contrast, all untreated animals (n = 15) had adhesions that could only be separated by sharp dissection (P < 0.001). The hydrogel adhered to the peritoneal wounds, gradually disappeared from the wounds within 7 days, and transformed into viscous fluid, being completely absorbed within 12 days. The parietal and visceral peritoneum were remesothelialized in about 5 and 9 days, respectively. The hydrogel prevented the formation of fibrinous adhesion and the invasion of fibroblasts. Also, along with the hydrogel degradation, a temporary inflammatory cell barrier was formed which could effectively delay the invasion of fibroblasts during the critical period of mesothelial regeneration.


CONCLUSION
The results suggested that PECE hydrogel could effectively prevent postsurgical intra-abdominal adhesions, which possibly result from the prevention of the fibrinous adhesion formation and the fibroblast invasion, the promotion of the remesothelialization, and the hydroflotation effect.
['Yang|Bing|B|', 'Gong|ChangYang|C|', 'Zhao|Xia|X|', 'Zhou|ShengTao|S|', 'Li|ZhengYu|Z|', 'Qi|XiaoRong|X|', 'Zhong|Qian|Q|', 'Luo|Feng|F|', 'Qian|ZhiYong|Z|']
D034841:Abdominal Cavity D000818:Animals D001672:Biocompatible Materials D004848:Epithelium D005260:Female D005355:Fibrosis D006651:Histocytochemistry D044367:Phase Transition D011091:Polyesters D011092:Polyethylene Glycols D011183:Postoperative Complications D051381:Rats D017208:Rats, Wistar D018709:Statistics, Nonparametric D013696:Temperature D000267:Tissue Adhesions
biocompatible thermosensitive barrier anti-adhesion