IPMK and β-catenin mediate PLC-β1-dependent signaling in myogenic differentiation.
In previous studies, we have reported that phospholipase C (PLC)-β1 plays a crucial role in myogenic differentiation and we determined the importance of its catalytic activity for the initiation of this process. Here we define the effectors that take part to its signaling pathway. We show that the Inositol Polyphosphate Multikinase (IPMK) is able to promote myogenic differentiation since its overexpression determines the up-regulation of several myogenic markers. Moreover, we demonstrate that IPMK activates the same cyclin D3 promoter region targeted by PLC-β1 and that IPMK-induced promoter activation relies upon c-jun binding to the promoter, as we have shown previously for PLC-β1. Furthermore, our data shows that IPMK overexpression causes an increase in β-catenin translocation and accumulation to the nuclei of differentiating myoblasts resulting in higher MyoD activation. Finally, we describe that PLC-β1 overexpression determines too an increase in β-catenin translocation and that PLC-β1, IPMK and β-catenin are mediators of the same signaling pathway since their overexpression results in cyclin D3 and myosin heavy chain (MYH) induction.
['Ramazzotti|Giulia|G|', 'Billi|Anna Maria|AM|', 'Manzoli|Lucia|L|', 'Mazzetti|Cristina|C|', 'Ruggeri|Alessandra|A|', 'Erneux|Christophe|C|', 'Kim|Seyun|S|', 'Suh|Pann-Ghill|PG|', 'Cocco|Lucio|L|', 'Faenza|Irene|I|']
D021581:Active Transport, Cell Nucleus D000818:Animals D015153:Blotting, Western D002454:Cell Differentiation D002460:Cell Line D002467:Cell Nucleus D056743:Cyclin D3 D051379:Mice D024510:Muscle Development D054799:Phospholipase C beta D017853:Phosphotransferases (Alcohol Group Acceptor) D011401:Promoter Regions, Genetic D015398:Signal Transduction D015533:Transcriptional Activation D051176:beta Catenin
IPMK β-catenin myogenic differentiation inositol phosphates phospholipase C-β1