High PTPRQ Expression and Its Relationship to Expression of PTPRZ1 and the Presence of KRAS Mutations in Colorectal Cancer Tissues.
BACKGROUND
The risk of sporadic colorectal cancer (CRC) is strongly influenced by Iifestyle, environmental and genetic factors. Protein tyrosine phosphatases belong to a group of enzymes whose role in CRC has not yet been intensively studied. They play an important role in activation/de-activation of many enzymes, influencing cell biology by catalyzing reactions opposing those catalyzed by kinases. Protein tyrosine phosphatase receptor-like type Q (PTPRQ) and protein tyrosine phosphatase receptor-like type Z polypeptide 1 (PTPRZ1) have both been shown to be important in development of many cancer types including CRC.


MATERIALS AND METHODS
The expression level of PTPRQ and PTPRZ1 was determined by real-time polymerase chain reaction in 16 CRC tissues obtained from patients diagnosed with adenocarcinoma coli.


RESULTS
We revealed a high level of PTPRQ expression (p=0.0080), as well as an association between expression levels of PTPRQ and PTPRZ1 (p<0.0001). Moreover PTPRQ expression was higher in tissues presenting with Kirsten rat sarcoma viral oncogene homolog (KRAS) mutation (p=0.0293).


CONCLUSION
We confirmed the contribution of PTPRZ1 and especially PTPRQ in CRC development, supporting the hypothesis that PTPRQ is a candidate oncogene, playing a crucial role in phosphorylation/dephosphorylation signaling pathways.
['Laczmanska|Izabela|I|', 'Karpinski|Pawel|P|', 'Gil|Justyna|J|', 'Laczmanski|Lukasz|L|', 'Bebenek|Marek|M|', 'Sasiadek|Maria M|MM|']
D000368:Aged D000369:Aged, 80 and over D014408:Biomarkers, Tumor D015179:Colorectal Neoplasms D004252:DNA Mutational Analysis D005260:Female D020022:Genetic Predisposition to Disease D006801:Humans D008297:Male D008875:Middle Aged D009154:Mutation D010641:Phenotype D016283:Proto-Oncogene Proteins p21(ras) D060888:Real-Time Polymerase Chain Reaction D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3 D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5 D020133:Reverse Transcriptase Polymerase Chain Reaction D015398:Signal Transduction
PTPRQ PTPRZ1 colorectal cancer Protein tyrosine phosphatase