PH-sauvagine from the skin secretion of Phyllomedusa hypochondrialis: A novel CRF-like peptide with smooth muscle contraction activity.
Amphibian skin, and particularly that of south/Central American phyllomedusine frogs, is supposed to be "a huge factory and store house of a variety of active peptides". The 40 amino acid amphibian CRF-like peptide, sauvagine, is a prototype member of a unique family of these Phyllomedusa skin peptides. In this study, we describe for the first time the structure of a mature novel peptide from the skin secretion of the South American orange-legged leaf frog, Phyllomedusa hypochondrialis, which belongs to the amphibian CRF/sauvagine family. Partial amino acid sequence from the N-terminal was obtained by automated Edman degradation with the following structure: pGlu-GPPISIDLNMELLRNMIEI-. The biosynthetic precursor of this novel sauvagine peptide, consisted of 85 amino acid residues and was deduced from cDNA library constructed from the same skin secretion. Compared with the standard sauvagine from the frog, Phyllomedusa sauvagei, this novel peptide was found to exert similar contraction effects on isolated guinea-pig colon and rat urinary bladder smooth muscle preparations.
['Zhou|Yu|Y|', 'Shaw|Chris|C|', 'Chen|Tianbao|T|']
D000595:Amino Acid Sequence D029845:Amphibian Proteins D000818:Animals D001001:Anura D001483:Base Sequence D003001:Cloning, Molecular D003106:Colon D015723:Gene Library D006168:Guinea Pigs D008297:Male D008969:Molecular Sequence Data D009119:Muscle Contraction D009130:Muscle, Smooth D036361:Peptide Hormones D051381:Rats D017208:Rats, Wistar D012867:Skin D001743:Urinary Bladder
Phyllomedusa hypochondrialis Peptide Smooth muscle Sauvagine CRF cDNA