Development of in vitro PIK3C3/VPS34 complex protein assay for autophagy-specific inhibitor screening.
Autophagy is an important catabolic program to respond to a variety of cellular stresses by forming a double membrane vesicle, autophagosome. Autophagy plays key roles in various cellular functions. Accordingly, dysregulation of autophagy is closely associated with diseases such as diabetes, neurodegenerative diseases, cardiomyopathy, and cancer. In this sense, autophagy is emerging as an important therapeutic target for disease control. Among the autophagy machineries, PIK3C3/VPS34 complex functions as an autophagy-triggering kinase to recruit the subsequent autophagy protein machineries on the phagophore membrane. Accumulating evidence showing that inhibition of PIK3C3/VPS34 complex successfully inhibits autophagy makes the complex an attractive target for developing autophagy inhibitors. However, one concern about PIK3C3/VPS34 complex is that many different PIK3C3/VPS34 complexes have distinct cellular functions. In this study, we have developed an in vitro PIK3C3/VPS34 complex monitoring assay for autophagy inhibitor screening in a high-throughput assay format instead of targeting the catalytic activity of the PIK3C3/VPS34 complex, which shuts down all PIK3C3/VPS34 complexes. We performed in vitro reconstitution of an essential autophagy-promoting PIK3C3/VPS34 complex, Vps34-Beclin1-ATG14L complex, in a microwell plate (96-well format) and successfully monitored the complex formation in many different conditions. This PIK3C3/VPS34 complex protein assay would provide a reliable tool for the screening of autophagy-specific inhibitors.
['Kim|Tae-Mi|TM|', 'Baek|Jong-Hyuk|JH|', 'Kim|Jeong Hee|JH|', 'Oh|Myung Sook|MS|', 'Kim|Joungmok|J|']
D001343:Autophagy D002478:Cells, Cultured D058546:Class III Phosphatidylinositol 3-Kinases D004797:Enzyme-Linked Immunosorbent Assay D057809:HEK293 Cells D057166:High-Throughput Screening Assays D006801:Humans D047428:Protein Kinase Inhibitors D013329:Structure-Activity Relationship
In vitro PIK3C3/VPS34 complex protein assay ATG14L Autophagy-specific inhibitor screening method