Evaluation of viral contamination in a baculovirus expression system.
Insect expression systems based on baculovirus are widely used for generating recombinant proteins. Here, the infectivity of baculoviruses under the physiological stresses of 'freeze-thaw' and sonication and the baculoviral contamination of recombinant proteins after protein purification were evaluated. Our findings suggest that Nonidet P-40 (NP-40) treatment of baculoviruses completely abolishes their infectivity and that recombinant proteins purified with affinity beads do not include infectious baculoviruses. We therefore suggest that baculovirus is completely inactivated by NP-40 treatment and that recombinant proteins are unlikely to be contaminated with infectious baculoviruses after their affinity purification.
['Ono|Chikako|C|', 'Hirano|Junki|J|', 'Okamoto|Toru|T|', 'Matsuura|Yoshiharu|Y|']
D016367:Baculoviridae D002846:Chromatography, Affinity D059018:DNA Contamination D005615:Freezing D005822:Genetic Vectors D017830:Octoxynol D011092:Polyethylene Glycols D011994:Recombinant Proteins D013010:Sonication D013312:Stress, Physiological D038301:Virus Inactivation
baculovirus insect expression system