Evolution of peripheral blood T lymphocyte subsets after allogenic or autologous hematopoietic stem cell transplantation.
With the aim to search for differences in T cell reconstitution after allogenic or autologous hematopoietic stem cell transplantation (HSCT), we characterized peripheral blood T-cell subsets by means of flow cytometry, in adult patients who had undergone either allogenic (n=23) or autologous (n=29) HSCT for the treatment of hematological malignancies. The patients were followed every 3 months for 21 months after HSCT. Compared to healthy controls (n=20 blood donors), the two transplanted groups displayed (i) a CD4 lymphopenia, (ii) a low percentage of naive T cells, (iii) high percentages of memory T cells and of activated T cells (HLA-DR+, CD25+) and high percentages of CD4 T cells with a high expression of CD25. The levels of TRECs (TCR rearrangement excision circles) were not significantly different between the two groups. In total, the differences of the nature and the speed of T lymphocyte reconstitution observed between the two patient groups were minor. This leads us to conclude that in allografted patients, lymphocyte activation as well as many other disturbances of subpopulations of peripheral blood lymphocytes are probably not related to the allogenicity of the graft, but are due to the expansion of T cells transfused with HSC and slow differentiation of T lymphocytes in the thymus progressively colonized by bone marrow-derived T-cell precursors.
['Puissant-Lubrano|Bénédicte|B|', 'Huynh|Anne|A|', 'Attal|Michel|M|', 'Blancher|Antoine|A|']
D000328:Adult D001775:Blood Circulation D015496:CD4-Positive T-Lymphocytes D002469:Cell Separation D005260:Female D005434:Flow Cytometry D006086:Graft vs Host Disease D019337:Hematologic Neoplasms D018380:Hematopoietic Stem Cell Transplantation D006801:Humans D007156:Immunologic Memory D053645:Interleukin-2 Receptor alpha Subunit D008213:Lymphocyte Activation D008297:Male D008875:Middle Aged D016176:T-Lymphocyte Subsets D014182:Transplantation, Autologous D014184:Transplantation, Homologous
Hematopoietic stem cell transplantation T lymphocyte reconstitution Allogenic HSCT Autologous HSCT