Anammox biofilm in activated sludge swine wastewater treatment plants.
We investigated anammox with a focus on biofilm in 10 wastewater treatment plants (WWTPs) that use activated sludge treatment of swine wastewater. In three plants, we found red biofilms in aeration tanks or final sedimentation tanks. The biofilm had higher anammox 16S rRNA gene copy numbers (up to 1.35 × 1012 copies/g-VSS) and higher anammox activity (up to 295 μmoL/g-ignition loss/h) than suspended solids in the same tank. Pyrosequencing analysis revealed that Planctomycetes accounted for up to 17.7% of total reads in the biofilm. Most of them were related to Candidatus Brocadia or Ca. Jettenia. The highest copy number and the highest proportion of Planctomycetes were comparable to those of enriched anammox sludge. Thus, swine WWTPs that use activated sludge treatment can fortuitously acquire anammox biofilm. Thus, concentrated anammox can be detected by focusing on red biofilm.
['Suto|Ryu|R|', 'Ishimoto|Chikako|C|', 'Chikyu|Mikio|M|', 'Aihara|Yoshito|Y|', 'Matsumoto|Toshimi|T|', 'Uenishi|Hirohide|H|', 'Yasuda|Tomoko|T|', 'Fukumoto|Yasuyuki|Y|', 'Waki|Miyoko|M|']
D000641:Ammonia D000693:Anaerobiosis D000818:Animals D018441:Biofilms D019149:Bioreactors D010084:Oxidation-Reduction D061328:Planctomycetales D012336:RNA, Ribosomal, 16S D012722:Sewage D013552:Swine D062065:Waste Water D018508:Water Purification
Anammox Biofilm Activated sludge Swine wastewater