Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats.
Rhodamine dyes have been banned as food additives due to their potential tumorigenicity. Rhodamine 110 is illegal as a food additive, although its pharmacokinetics have not been characterized, and no accurate bioanalytical methods are available to quantify rhodamine 110. The aim of this study was to develop and validate a fast, stable, and sensitive method to quantify rhodamine 110 using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to assess its pharmacokinetics and organ distribution in awake rats. Rhodamine 110 exhibited linear pharmacokinetics and slow elimination after oral administration. Furthermore, its oral bioavailability was approximately 34-35%. The distribution in the liver and kidney suggests that these organs are primarily responsible for rhodamine 110 metabolism and elimination. Our investigation describes the pharmacokinetics and a quantification method for rhodamine 110, improving our understanding of the food safety of rhodamine dyes.
['Jiang|Shiau-Han|SH|', 'Cheng|Yung-Yi|YY|http://orcid.org/0000-0001-7473-2835', 'Huo|Teh-Ia|TI|', 'Tsai|Tung-Hu|TH|http://orcid.org/0000-0002-9007-2547']
D000818:Animals D002851:Chromatography, High Pressure Liquid D005505:Food Coloring Agents D007668:Kidney D008099:Liver D008297:Male D051381:Rats D017207:Rats, Sprague-Dawley D012235:Rhodamines D053719:Tandem Mass Spectrometry D014018:Tissue Distribution
pharmacokinetics rhodamine 110 rhodamine dyes food additives HPLC-MS/MS toxicokinetics