Characteristics of resveratrol and serotonin on antioxidant capacity and susceptibility to oxidation of red blood cells in stored human blood in a time-dependent manner.
Objective In stored red blood cells (RBCs), which are used in diseases (e.g., acute blood loss and leukaemia), storage lesions arise by oxidative stress and other factors over time. This study investigated the protective effects of resveratrol and serotonin on stored RBCs. Methods Blood from each donor (n = 10) was placed in different bags containing 70 mL of citrate phosphate dextrose (total volume: 500 mL) and divided into three groups (n = 30): control, 60 µg/mL resveratrol, and 60 µg/mL serotonin. Malondialdehyde (MDA) and glutathione (GSH) levels, activity of glutathione peroxidase (GSH-Px), catalase, and carbonic anhydrase (CA), and susceptibility to oxidation in RBCs, and pH in whole blood were measured at baseline and on days 7, 14, 21, and 28. Results MDA levels and susceptibility to oxidation were increased in all three groups time-dependently, but this increase was greater in the serotonin group than in the other groups. Activity of GSH-Px, CAT, and CA, as well as GSH levels, were decreased in the control and serotonin groups time-dependently, but were significantly preserved in the resveratrol group. The pH was decreased in all groups time-dependently. Conclusion Our study shows that resveratrol attenuates susceptibility to oxidation of RBCs and protects their antioxidant capacity, and partially preserves CA activity time-dependently.
['Huyut|Zübeyir|Z|', 'Şekeroğlu|Mehmet Ramazan|MR|', 'Balahoroğlu|Ragıp|R|', 'Huyut|Mehmet Tahir|MT|']
D000925:Anticoagulants D000975:Antioxidants D002256:Carbonic Anhydrases D002374:Catalase D002951:Citrates D004912:Erythrocytes D005947:Glucose D005978:Glutathione D005979:Glutathione Peroxidase D006801:Humans D015227:Lipid Peroxidation D008315:Malondialdehyde D061251:Primary Cell Culture D000077185:Resveratrol D012701:Serotonin D013267:Stilbenes D013482:Superoxide Dismutase
resveratrol serotonin Antioxidants oxidation storage lesions stored RBCs chronic storage