Factors for C-Kit Expression in Cardiac Outgrowth Cells and Human Heart Tissue.
We determined the factors associated with the expression of c-kit in the heart and the proliferation of c-kit-positive (c-kitpos) cardiac stem cells among the outgrowth cells cultured from human cardiac explants.Samples of the right atrium (RA), left atrium (LA), and left ventricle obtained from patients during open-heart surgery were processed for cell culture of outgrowth cells and tissue analysis. The total number of growing cells and the population of c-kitpos cells were measured and compared with c-kit expression in native tissues and characteristics of the patients according to the region of the heart.We analyzed 452 samples from 334 patients. Atrial fibrillation (AF) in the patients reduced the number of outgrowth cells from the RA and LA, and aging was a co-factor for the LA. The c-kitpos population from the RA was associated with serum brain natriuretic peptide (BNP). C-kit expression in native tissue was also associated with BNP expression. However, we observed no relationship in expression between outgrowth cells and native tissue. In addition, the RA tissue provided the highest number of c-kitpos cells, and the left ventricle provided the lowest.C-kit was weakly expressed in response to damage. In addition, no correlation between outgrowth cells and native tissue was found for c-kit expression.
['Matsushita|Satoshi|S|', 'Minematsu|Kazuo|K|', 'Yamamoto|Taira|T|', 'Inaba|Hirotaka|H|', 'Kuwaki|Kenji|K|', 'Shimada|Akie|A|', 'Yokoyama|Yasutaka|Y|', 'Amano|Atsushi|A|']
D053687:Adult Stem Cells D000368:Aged D000369:Aged, 80 and over D005260:Female D006801:Humans D008297:Male D008875:Middle Aged D009206:Myocardium D020097:Natriuretic Peptide, Brain D061251:Primary Cell Culture D019009:Proto-Oncogene Proteins c-kit
Cardiac stem cells Atrial fibrillation Aging Brain natriuretic peptide Heart injury Self-repair