Nanopurpurin-based photodynamic therapy destructs extracellular matrix against intractable tumor metastasis.
Nanomaterials-based photodynamic therapy (PDT) has been used to treat malignant cells. However, the intrinsic impact of nanomaterials-based PDT on mechanical properties of intractable tumor cells is not well understood. Herein, we demonstrated that the mechanical forces of Taxol-resistant tumor cells were decreased by nanopurpurin-based PDT destructing extracellular matrix (ECM), increasing therapy sensitivity and repressing tumor metastasis. Combining FIRMS and general confocal microscope, we observed that the disruption of ECM by photodynamic reaction of P18-nanoconfined liposome (P18⊂L) induced a decrease of adhesion force and biomechanical properties of Taxol-resistant cells through the attenuation of actomyosin-based contractility thereby inhibiting cell migration and metastasis in vivo. Moreover, the destroyed ECM by P18⊂L PDT increased the therapy sensitivity. A clearer understanding of the effect of nanopurpurin-based PDT on mechanical properties and behaviors of intractable tumor cells will provide new and important basis for developing new therapeutic strategies.
['Zhang|Di|D|', 'Feng|Feng|F|', 'Li|Qilong|Q|', 'Wang|Xiuyu|X|', 'Yao|Li|L|']
D000072283:A549 Cells D000205:Actomyosin D000818:Animals D023102:Anoikis D000880:Anthraquinones D017209:Apoptosis D002448:Cell Adhesion D002465:Cell Movement D004129:Dimethylpolysiloxanes D005109:Extracellular Matrix D005260:Female D064593:Heterografts D006801:Humans D008081:Liposomes D008807:Mice, Inbred BALB C D008819:Mice, Nude D049329:Nanostructures D009362:Neoplasm Metastasis D010778:Photochemotherapy
Photodynamic therapy Extracellular matrix Tumor metastasis Adhesion Biomechanical forces