Production of the Fusarium Mycotoxin Moniliformin by Penicillium melanoconidium.
Moniliformin is a mycotoxin produced by several cereal associated Fusaria. Here, we show for the first time that moniliformin can be produced by the cereal fungus, Penicillium melanoconidium (4 out of 4 strains), but not in the related species in the Viridicata series. Moniliformin was detected in 10 out of 11 media: two agars and several cereal and bean types. Moniliformin was identified by a novel mixed-mode anionic exchange reversed phase chromatographic method which was coupled to both tandem mass spectrometry (MS) and high resolution MS. Mixed-mode chromatography showed superior peak shape compared to that of HILIC and less matrix interference compared to that of reversed phase chromatography, but during a large series of analyses, the column was fouled by matrix interferences. Wheat and beans were artificially infected by P. melanoconidium containing up to 64 and 11 mg/kg moniliformin, respectively, while penicillic acid, roquefortine C, and penitrem A levels in wheat were up to 1095, 38, and 119 mg/kg, respectively.
['Hallas-Møller|Magnus|M|', 'Nielsen|Kristian Fog|KF|', 'Frisvad|Jens Christian|JC|']
D002851:Chromatography, High Pressure Liquid D003503:Cyclobutanes D002523:Edible Grain D005506:Food Contamination D005670:Fusarium D009183:Mycotoxins D010407:Penicillium D053719:Tandem Mass Spectrometry
moniliformin Penicillium melanoconidium mixed-mode chromatography penicillic acid roquefortine C penitrem A LC-MS/MS wheat grain white beans