Long noncoding RNA ERICD interacts with ARID3A via E2F1 and regulates migration and proliferation of osteosarcoma cells.
Long noncoding RNA (lncRNA) dysregulation is known to be taking part in majority of cancers, including osteosarcoma. In one of our previous studies, we showed that lncRNA MEG3 is being regulated by microRNA-664a (miR-664a) suppresses the migratory potential of osteosarcoma cells (U-2OS). We now report a novel lncRNA, namely, ERICD, which is linked to the transcription factor AT-rich interaction domain 3A (ARID3A) in U-2OS cells. We show that ARID3A binds to ERICD and indirectly interacts with each other via the E2F transcription factor 1 (E2F1). Furthermore, small interfering RNA (siRNA)-mediated knockdown of ERICD inhibited cell migration, formation of colonies, and proliferation in U-2OS cells. Overexpression of ARID3A inhibited cell migration, colony formation, and proliferation, whereas siRNA-mediated knockdown of ARID3A promoted cell migration, colony formation, and proliferation. Our findings indicate that ARID3A and lncRNA ERICD have plausible tumor suppressive and oncogenic functions, respectively, in osteosarcoma. Our data demonstrate the converse interaction between ARID3A and lncRNA ERICD that target DNA-binding proteins and dysregulation of their expression through E2F1 augments osteosarcoma progression. The cell rescue experiment also indicated E2F1 to be involved in the regulation of ARID3A and ERICD.
['Arman|Kaifee|K|http://orcid.org/0000-0002-4667-4655', 'Saadat|Khandakar A S M|KASM|', 'Igci|Yusuf Z|YZ|http://orcid.org/0000-0001-9187-3728', 'Bozgeyik|Esra|E|', 'Ikeda|Masa-Aki|MA|', 'Cakmak|Ecir A|EA|', 'Arslan|Ahmet|A|']
D017209:Apoptosis D001859:Bone Neoplasms D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D004268:DNA-Binding Proteins D050687:E2F1 Transcription Factor D015972:Gene Expression Regulation, Neoplastic D006801:Humans D012516:Osteosarcoma D062085:RNA, Long Noncoding D014157:Transcription Factors
ARID3A migration osteosarcoma colony formation lncRNA ERICD DNA-binding lncRNA