Identification of microRNAs for regulating adenosine monophosphate-activated protein kinase expression in immature boar Sertoli cells in vitro.
Adenosine monophosphate-activated protein kinase (AMPK) plays a key role in cellular energy homeostasis and cell proliferation. MicroRNAs (miRNAs) function as posttranscriptional regulators of gene expression in biological processes. It is unclear to whether miRNAs are involved in AMPK-regulated Sertoli cell (SC) proliferation. To further understand the regulation of miRNAs in the immature boar SC proliferation, 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR) was added to activate AMPK. By an Illumina small RNA deep sequencing, we obtained sequences and relative expression levels of 272 known mature miRNAs, among which 9 miRNAs were significantly upregulated whereas 16 miRNAs were downregulated following the AICAR treatment. The results identified 38 conserved miRNAs, with 8 significantly downregulated miRNAs whereas no upregulated miRNAs. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses suggested that miR-1285 was involved in many activities and pathways associated with cell proliferation via targeting on AMPKα2. We validated that AICAR significantly downregulated miR-1285 level in SCs. Transfection of miR-1285 mimic increased the SC viability and cell cycle progression but reduced AMPKα2 mRNA and protein levels, indicating that miR-1285 is involved in the immature boar SC proliferation via downregulating AMPKα2 expression.
['Zhang|Jiao Jiao|JJ|0000-0002-2699-6729', 'Yang|Wei Rong|WR|0000-0002-4495-5250', 'Wang|Yi|Y|', 'Chen|Liang|L|', 'Jeong|Dong Kee|DK|', 'Wang|Xian Zhong|XZ|0000-0002-4540-1540']
D055372:AMP-Activated Protein Kinases D000620:Aminoimidazole Carboxamide D000818:Animals D049109:Cell Proliferation D005786:Gene Expression Regulation D008297:Male D035683:MicroRNAs D012265:Ribonucleotides D012708:Sertoli Cells D013552:Swine
Sertoli cell AMPK miRNA sequencing miR-1285