Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
This study was carried out to study the effects of selenylation on physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum. The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method. Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides. FT-IR spectroscopy showed that a new absorption peak appeared at 675 cm-1 in Se-SPP probably due to the substitution of selenyl groups. Bioactivity assay showed that Se-SPP exhibited higher scavenging radical activities and ferrous ion chelating activities than native SPP. Compared with SPP and acarbose, Se-SPP showed more significantly inhibitory effect on α-glucosidase activity in a noncompetitive inhibition type. The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively. These results suggest that Se-SPP can be used to develop a new selenium-complementary ingredient in functional foods.
['Xiao|Heng|H|', 'Chen|Chun|C|', 'Li|Chao|C|', 'Huang|Qiang|Q|', 'Fu|Xiong|X|']
D000975:Antioxidants D052160:Benzothiazoles D001713:Biphenyl Compounds D002614:Chelating Agents D055598:Chemical Phenomena D065089:Glycoside Hydrolase Inhibitors D007004:Hypoglycemic Agents D007501:Iron D007700:Kinetics D008970:Molecular Weight D010851:Picrates D011134:Polysaccharides D044647:Sargassum D012643:Selenium D013451:Sulfonic Acids
Antioxidant Hypoglycemic Polysaccharide Selenylation Structure