The Lymphoma-Associated Macrophage to Hodgkin-Reed-Sternberg Cell Ratio Is a Poor Prognostic Factor in Classic Hodgkin Lymphoma Patients.
BACKGROUND
Despite the relatively high rate of curability, approximately 20% to 30% of patients with classic Hodgkin lymphoma relapse. Hodgkin-Reed-Sternberg (HRS) cells:lymphoma-associated macrophages (LAMs) cross talk promotes tumor growth and resistance to therapy. The aim of the study was to assess the prognostic role of the LAM to HRS ratio (LHR) in lymph node biopsies using a novel automated system for scanning large sample areas.


PATIENTS AND METHODS
High-quality tissue samples obtained from 71 patients and stained with anti-CD30 and anti-CD68 were analyzed using the TissueFAXS (TissueGnostics).


RESULTS
A high LHR was associated with inferior 5-year progression-free survival (PFS; 50.0% vs. 79.3%; P = .032) and overall survival (OS; 65.4% vs. 92.3%; P = .012). Multivariate Cox regression identified the high LHR as an unfavorable prognostic factor for PFS (hazard ratio [HR], 3.07; P = .029) and OS (HR, 4.56; P = .025).


CONCLUSION
A high LHR at diagnosis is associated with a higher risk of lymphoma progression or death. Automated image analysis is a new tool that can overcome technical limitations of by microarray samples in lymphomas with high intratumor heterogeneity.
['Procházka|Vít|V|', 'Papajík|Tomáš|T|', 'Dýšková|Tereza|T|', 'Dihel|Martin|M|', 'Brychtová|Světlana|S|', 'Prouzová|Zuzana|Z|', 'Kriegová|Eva|E|', 'Lukášová|Marie|M|', 'Hanáčková|Veronika|V|']
D000293:Adolescent D000328:Adult D000368:Aged D000369:Aged, 80 and over D015703:Antigens, CD D015214:Antigens, Differentiation, Myelomonocytic D000971:Antineoplastic Combined Chemotherapy Protocols D001706:Biopsy D018572:Disease-Free Survival D005260:Female D006689:Hodgkin Disease D006801:Humans D017730:Ki-1 Antigen D008198:Lymph Nodes D008264:Macrophages D008297:Male D008875:Middle Aged D009364:Neoplasm Recurrence, Local D011379:Prognosis D016539:Reed-Sternberg Cells D055815:Young Adult
Hodgkin lymphoma Microenvironment Prognosis