The effect of flavonoid quercetine on the indices of nitric oxide system in rats with chronic enterocolitis combined with streptozotocin-induced diabetes.
OBJECTIVE
Introduction: Chronic hyperglycemia is accompanied by significant physiological, biochemical, and histological changes, e.g. development of oxidative and nitrosative stress that affects the motor activity of the intestine. The aim: The present study was designed to evaluate the indices of nitric oxide (NO) system in blood serum and a colon tissue supernatant of rats with chronic enterocolitis combined with streptozotocin-induced diabetes.


PATIENTS AND METHODS
Materials and methods: The total NOS activity was performed by monitoring the rate of conversion of L-arginine into citrulline. The total contents of NO metabolites was assessed by evaluation of their amount, which included nitrite ions that were previously presented in the sample (NO2-) and also nitrate ions reducted to nitrites (NO3-).


RESULTS
Results and conclusions: Thus, in rats with modeled chronic enterocolitis activation of nitroxydergic process in blood serum and colon tissue has been established. Herewith more pronounced intensification of nitroxydergic processes was observed in rats with chronic enterocolitis combined with streptozotocin-induced diabetes. The liposomal form of quercetin - Lipoflavon significantly reduces nitrosative stress in rats with chronic enterocolitis combined with streptozotocin-induced diabetes.
['Krynytska|Іnna|І|', 'Kushynska|Mariia|M|', 'Pavlenko|Iryna|I|', 'Marushchak|Mariya|M|']
D000818:Animals D001120:Arginine D002956:Citrulline D003921:Diabetes Mellitus, Experimental D004760:Enterocolitis D009569:Nitric Oxide D009573:Nitrites D000073039:Nitrosative Stress D011794:Quercetin D051381:Rats D013311:Streptozocin
quercetin nitric oxide enterocolitis diabetes NO-synthase