The effect of trimetazidine in reducing the ischemia-reperfusion injury in rat epigastric skin flaps.
BACKGROUND
Ischemia-reperfusion injury may lead to insufficient microcirculation and results in partial flap loss during the free flap surgeries.


OBJECTIVE
This study aimed to investigate the effect of trimetazidine (TMZ) on oxidative stress, inflammation and histopathological changes, using the epigastric skin flap model in rats.


METHODS
40 male Wistar rats were used, that were divided into four groups. Control group, non-treated ischemic (I/R)-group and two trimetazidine treated groups (preischemically, postischemically) were established. To create ischemia in the skin flap, the superficial epigastric vessels were clamped for six hours, followed by twenty-four hours of reperfusion. Blood samples and biopsies from skin flaps were collected at the end of the reperfusion period. The inflammatory response, the degree of oxidative stress (by measuring the plasma level of malondialdehyde (MDA), reduced glutathione (GSH); sulfhydryl (-SH) groups) and histopathological changes were evaluated.


RESULTS
Inflammatory response, and oxidative stress were significantly attenuated in the trimetazidine treated groups, compared to the non-treated ischemic group. Histopathological findings were also correlated with the biochemical results.


CONCLUSION
In our study trimetazidine could reduce the ischaemia-reperfusion injury, even after an unexpected ischemic period, so it is a promising drug during free tissue transfer, replantation or during revascularization procedures in the future.
['Petrovics|Laura|L|', 'Nagy|Tibor|T|', 'Hardi|Peter|P|', 'Bognar|Laura|L|', 'Pavlovics|Gabor|G|', 'Tizedes|Gyorgy|G|', 'Takacs|Ildiko|I|', 'Jancso|Gabor|G|']
D000818:Animals D008297:Male D051381:Rats D017208:Rats, Wistar D015427:Reperfusion Injury D013524:Surgical Flaps D014292:Trimetazidine D014665:Vasodilator Agents
trimetazidine free flaps oxidative stress inflammation Ischaemia-reperfusion injury reconstruction