Structural characterization and antidiabetic potential of a novel heteropolysaccharide from Grifola frondosa via IRS1/PI3K-JNK signaling pathways.
A novel heteropolysaccharide from Grifola frondosa named GFP-W has been isolated and purified by DEAE Sephadex A-52 chromatography. Gas chromatography, fourier transform infrared spectroscopy, one-dimensional (1H- and 13C-) and two-dimensional (1H-1H COSY, 1H-13C HSQC, and 1H-13C HMBC) nuclear magnetic resonance spectroscopy were used to characterize its structure. The average molecular weight of GFP-W was 66.1 kDa. GFP-W mainly contained four kinds of linkage type units as β-D-GlcpA→, 1,2,6-α-Gal, →2)-α-Manp→, and →3)-α-L-Fucp-(1→. It could significantly increase the uptake of glucose in dexamethasone induced insulin resistant HepG2 cells by improving the mRNA and protein expression of insulin receptor substrate 1, phosphatidylinositol-3-kinase, and glucose transporter 4 upregulation and c-Jun N-terminal Kinase 1 downregulation. Moreover, antidiabetic activities of GFP-W were associated with significant changes in the extent of protein lysine acetylation, crotonylation, and succinylation levels. Our results provide a new hypoglycemic therapeutic role and an in-depth analysis on molecular mechanisms upon polysaccharides from G. frondosa.
['Chen|Yuqing|Y|', 'Liu|Yuanyuan|Y|', 'Sarker|Md Moklesur Rahman|MMR|', 'Yan|Xin|X|', 'Yang|Chengfeng|C|', 'Zhao|Lina|L|', 'Lv|Xucong|X|', 'Liu|Bin|B|', 'Zhao|Chao|C|']
D000818:Animals D002470:Cell Survival D003921:Diabetes Mellitus, Experimental D062610:Fungal Polysaccharides D005947:Glucose D048691:Grifola D056945:Hep G2 Cells D006801:Humans D007004:Hypoglycemic Agents D055504:Insulin Receptor Substrate Proteins D007333:Insulin Resistance D008297:Male D008813:Mice, Inbred ICR D048055:Mitogen-Activated Protein Kinase 8 D019869:Phosphatidylinositol 3-Kinases D015398:Signal Transduction
Structural characterization Antidiabetic Grifola frondosa polysaccharide Insulin signaling pathway Protein lysine modification