Identification of two novel foot-and-mouth disease virus cytotoxic T lymphocyte epitopes that can bind six SLA-I proteins.
Currently available vaccines from inactivated foot-and-mouth disease virus (FMDV) only protect animals by inducing neutralizing antibodies. A vaccine that contains cytotoxic T lymphocytes (CTL) epitopes to induce strong CTL responses might protect animals more effectively. Herein, we used swine leukocyte antigen class I (SLAI) proteins derived from six different strains of domestic pigs to screen and identify shared FMDV CTL epitopes. Four potential FMDV CTL epitopes (Q01, Q02, AS3, and QA4) were confirmed by mass spectrometry. We also determined the antigenicity of these epitopes to elicit cell-mediated immunoresponse by the ELISPOT and CTL assays. Among the four peptides, Q01 and QA4 were found to bind all six SLA-I proteins with strong affinity and elicit significant activity of CTL (P < 0.01). We conclude that Q01 and QA4 peptides are novel shared epitopes that can be recognized by all six SLA-I molecules on representative CTLs.
['Gao|Feng-Shan|FS|', 'Zhai|Xiao-Xin|XX|', 'Jiang|Ping|P|', 'Zhang|Qiang|Q|', 'Gao|Hua|H|', 'Li|Zi-Bin|ZB|', 'Han|Yong|Y|', 'Yang|Jie|J|', 'Zhang|Zong-Hui|ZH|']
D000818:Animals D057134:Antibodies, Neutralizing D018984:Epitopes, T-Lymphocyte D004926:Escherichia coli D005536:Foot-and-Mouth Disease D029544:Foot-and-Mouth Disease Virus D008297:Male D011485:Protein Binding D013045:Species Specificity D013552:Swine D013602:T-Lymphocytes, Cytotoxic
Foot-and-mouth disease virus Cytotoxic T lymphocyte Epitope Swine leukocyte antigen Peptide Major histocompatibility complex