DNA Methylation Patterns in the Early Human Embryo and the Epigenetic/Imprinting Problems: A Plea for a More Careful Approach to Human Assisted Reproductive Technology (ART).
An increasing number of publications indicate that babies born after IVF (in vitro fertilization) procedures have higher rates of anomalies related to imprinting/epigenetic changes, which may be attributed to suboptimal culture conditions. Appropriate maintenance of DNA methylation during the first few days of an in vitro culture requires a supply of methyl donors, which are lacking in current in vitro culture systems. The absence of protection against oxidative stress in the culture increases the risks for errors in methylation. A decrease in the methylation processes is sometimes observed immediately post fertilization, due to delays that occur during the maternal⁻zygotic transition period. Care should be exercised in ART (assisted reproductive technology) procedures in order to avoid the risk of generating errors in methylation during the in vitro culture period immediately post fertilization, which has an impact on imprinting/epigenetics. Formulation of IVF culture media needs to be re-assessed in the perspective of current knowledge regarding embryo physiology.
['Menezo|Yves|Y|0000-0001-6861-8905', 'Clément|Patrice|P|', 'Dale|Brian|B|']
D018929:Cell Culture Techniques D019175:DNA Methylation D004622:Embryo, Mammalian D044127:Epigenesis, Genetic D005307:Fertilization in Vitro D018507:Gene Expression Regulation, Developmental D018392:Genomic Imprinting D006801:Humans D018384:Oxidative Stress D015053:Zygote
methylation imprinting IVF culture conditions epigenesis maternal to zygotic transition