Involvement of RNA granule proteins in meiotic silencing by unpaired DNA.
In Neurospora crassa, expression from an unpaired gene is suppressed by a mechanism known as meiotic silencing by unpaired DNA (MSUD). MSUD utilizes common RNA interference (RNAi) factors to silence target mRNAs. Here, we report that Neurospora CAR-1 and CGH-1, homologs of two Caenorhabditis elegans RNA granule components, are involved in MSUD. These fungal proteins are found in the perinuclear region and P-bodies, much like their worm counterparts. They interact with components of the meiotic silencing complex (MSC), including the SMS-2 Argonaute. This is the first time MSUD has been linked to RNA granule proteins.
['Xiao|Hua|H|', 'Vierling|Michael M|MM|', 'Kennedy|Rana F|RF|', 'Boone|Erin C|EC|', 'Decker|Logan M|LM|', 'Sy|Victor T|VT|', 'Haynes|Jackson B|JB|', 'Williams|Michelle A|MA|', 'Shiu|Patrick K T|PKT|']
D004271:DNA, Fungal D020868:Gene Silencing D008540:Meiosis D009492:Neurospora crassa D012313:RNA D034622:RNA Interference
RNA granules Neurospora crassa meiotic silencing by unpaired DNA (MSUD) RNA interference (RNAi) P-bodies