Cortical microcirculatory disturbance in the super acute phase of subarachnoid hemorrhage - In vivo analysis using two-photon laser scanning microscopy.
OBJECTIVE
Subarachnoid hemorrhage (SAH) causes cerebral ischemia and drastically worsens the clinical status at onset. However, the arterial flow is surprisingly well maintained on the cerebral surface. We investigated cortical microcirculatory changes in the super acute phase of SAH using two-photon laser scanning microscopy (TPLSM).


METHODS
SAH was induced at the skull base in 10 mice using a prone endovascular perforation model. Before SAH, and 1, 2, 5, 10, 20, 30 and 60min after SAH, the cortical microcirculation was observed with TPLSM through a cranial window. Diameters of penetrating and precapillary arterioles were measured and red blood cell (RBC) velocities in precapillary arterioles were analyzed using a line-scan method after administration of Q-dot 655 nanocrystals.


RESULTS
One minute after SAH, RBC velocity and flow in precapillary arterioles drastically decreased to <20% of the pre-SAH values, while penetrating and precapillary arterioles dilated significantly. Subsequently, the arterioles either dilated or constricted inconsistently for 60min with continual decreases in RBC velocity and flow in the arterioles, suggesting neurovascular dysfunction.


CONCLUSION
SAH caused sudden worsening of the cortical arteriolar velocity and flow at onset. The neurovascular unit cannot function sufficiently to maintain cortical microcirculatory flow in the super acute phase of SAH.
['Ishikawa|Mami|M|', 'Kajimura|Mayumi|M|', 'Morikawa|Takayuki|T|', 'Tsukada|Kosuke|K|', 'Tsuji|Toshiyuki|T|', 'Kusaka|Gen|G|', 'Tanaka|Yuichi|Y|', 'Suematsu|Makoto|M|']
D000704:Analysis of Variance D000818:Animals D001253:Astrocytes D002540:Cerebral Cortex D002560:Cerebrovascular Circulation D004195:Disease Models, Animal D049452:Green Fluorescent Proteins D021621:Imaging, Three-Dimensional D008297:Male D051379:Mice D008822:Mice, Transgenic D008833:Microcirculation D018613:Microscopy, Confocal D013345:Subarachnoid Hemorrhage D013997:Time Factors
Subarachnoid hemorrhage Ischemia Microcirculation Neurovascular function Vasodilation