Collagen synthesis promoting pullulan-PEI-ascorbic acid conjugate as an efficient anti-cancer gene delivery vector.
Cationized pullulan (pullulan-PEI; PP) was synthesized and further modified with an anti-oxidant molecule, ascorbic acid (PPAA) at various ratios. The nanoplexes formed at an optimum ratio of 4:1 was within a size of 150nm and had a zeta potential of 9-14mV. The nanoplexes at this ratio was used for further investigations. The cell internalization and transfection efficiency of these nanoplexes were determined in presence of serum. The internalization and transfection efficiency were found to be unaffected by the presence of fetal bovine serum. Another interesting observation was that this polymer was found to have collagen synthesis promoting property. The collagen synthesis effect of these polymers was quantified and observed that PPAA3 promoted the highest. Transfection efficiency was evaluated by assessing the p53 gene expression in C6 rat glioma cells and cell death was quantified to be 96% by flow cytometry, thus establishing the high efficacy of this polymer.
['Ambattu|Lizebona August|LA|', 'Rekha|M R|MR|']
D000818:Animals D001205:Ascorbic Acid D045744:Cell Line, Tumor D003094:Collagen D004247:DNA D018014:Gene Transfer Techniques D015316:Genetic Therapy D005822:Genetic Vectors D005910:Glioma D005936:Glucans D049329:Nanostructures D011094:Polyethyleneimine D051381:Rats D014162:Transfection D016159:Tumor Suppressor Protein p53
Gene delivery Pullulan Ascorbic acid p53 Polyethylene imine