A Quest of Great Importance-Developing a Broad Spectrum Escherichia coli Phage Collection.
Shigella ssp. and enterotoxigenic Escherichia coli are the most common etiological agents of diarrheal diseases in malnourished children under five years of age in developing countries. The ever-growing issue of antibiotic resistance and the potential negative impact of antibiotic use on infant commensal microbiota are significant challenges to current therapeutic approaches. Bacteriophages (or phages) represent an alternative treatment that can be used to treat specific bacterial infections. In the present study, we screened water samples from both environmental and industrial sources for phages capable of infecting E. coli laboratory strains within our collection. Nineteen phages were isolatedand tested for their ability to infect strains within the ECOR collection and E. coli O157:H7 Δstx. Furthermore, since coliphages have been reported to cross-infect certain Shigella spp., we also evaluated the ability of the nineteen phages to infect a representative Shigella sonnei strain from our collection. Based on having distinct (although overlapping in some cases) host ranges, ten phage isolates were selected for genome sequence and morphological characterization. Together, these ten selected phages were shown to infect most of the ECOR library, with 61 of the 72 strains infected by at least one phage from our collection. Genome analysis of the ten phages allowed classification into five previously described genetic subgroups plus one previously underrepresented subgroup.
['Kaczorowska|Joanna|J|', 'Casey|Eoghan|E|', 'Neve|Horst|H|0000-0003-0295-2016', 'Franz|Charles M A P|CMAP|', 'Noben|Jean-Paul|JP|0000-0003-3368-5686', 'Lugli|Gabriele A|GA|', 'Ventura|Marco|M|', 'Sinderen|Douwe van|DV|', 'Mahony|Jennifer|J|']
D003090:Coliphages D004926:Escherichia coli D019453:Escherichia coli O157 D014644:Genetic Variation D016679:Genome, Viral D058507:Host Specificity D040901:Proteomics D012760:Shigella D014764:Viral Proteins D014871:Water Microbiology
Escherichia coli Shigella ssp. bacteriophage host range phage therapy