Heterologous expression and functional characterization of a GH10 endoxylanase from Aspergillus fumigatus var. niveus with potential biotechnological application.
Xylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving the accessibility of cellulose for cellulases. In this work, an endo-β-1,4-xylanase from Aspergillus fumigatus var. niveus (AFUMN-GH10) was successfully expressed. The structural analysis and biochemical characterization showed this AFUMN-GH10 does not contain a carbohydrate-binding module. The enzyme retained its activity in a pH range from 4.5 to 7.0, with an optimal temperature at 60 °C. AFUMN-GH10 showed the highest activity in beechwood xylan. The mode of action of AFUMN-GH10 was investigated by hydrolysis of APTS-labeled xylohexaose, which resulted in xylotriose and xylobiose as the main products. AFUMN-GH10 released 27% of residual xylan from hydrothermally-pretreated corn stover and 14% of residual xylan from hydrothermally-pretreated sugarcane bagasse. The results showed that environmentally friendly pretreatment followed by enzymatic hydrolysis with AFUMN-GH10 in low concentration is a suitable method to remove part of residual and recalcitrant hemicellulose from biomass.
['Velasco|Josman|J|', 'Oliva|Bianca|B|', 'Mulinari|Evandro José|EJ|', 'Quintero|Leidy Patricia|LP|', 'da Silva Lima|Awana|A|', 'Gonçalves|Aline Larissa|AL|', 'Gonçalves|Thiago Augusto|TA|', 'Damasio|André|A|', 'Squina|Fabio Marcio|FM|', 'Ferreira Milagres|Adriane Maria|AM|', 'Abdella|Asmaa|A|', 'Wilkins|Mark R|MR|', 'Segato|Fernando|F|']
Endoxylanase Corn stover Sugarcane bagasse Filamentous fungi Xylooligosaccharides