A genome-wide identification and classification of basic helix-loop-helix genes in the jewel wasp, Nasonia vitripennis (Hymenoptera: Pteromalidae).
Basic helix-loop-helix (bHLH) proteins are highly conserved DNA-binding transcription factors of a large superfamily. Animal bHLH proteins play important regulatory roles in various developmental processes such as neurogenesis, myogenesis, heart development, and hematopoiesis. The jewel wasp (Nasonia vitripennis) is a good model organism of hymenoptera insects for studies of developmental and evolutionary genetics. In this study, we identified 48 bHLH genes in the genome of N. vitripennis. According to phylogenetic analysis, based on N. vitripennis bHLH (NvbHLH) motif sequences and structural domain distribution in their full-length protein sequences, the identified NvbHLH genes were classified into 36 bHLH families with 19, 12, 9, 1, 6, and 1 member(s) in groups A, B, C, D, E, and F, respectively. Our classification to the identified NvbHLH family members confirms GenBank annotations for 21 of the 48 NvbHLH proteins and provides useful information for further characterization and annotation of the remaining 27 NvbHLH proteins. Compared to other insect species, N. vitripennis has the lowest number of bHLH family members. No NvbHLH members have been found in the families Net, MyoRa, and PTFa, while all other insect species have at least one member in each of the families. These data constitute a solid basis for further investigations into the functions of bHLH proteins in developmental regulation of N. vitripennis.
['Liu|Xiao-Ting|XT|', 'Wang|Yong|Y|', 'Wang|Xu-Hua|XH|', 'Tao|Xia-Fang|XF|', 'Yao|Qin|Q|', 'Chen|Ke-Ping|KP|']
D000818:Animals D051792:Basic Helix-Loop-Helix Transcription Factors D017344:Genes, Insect D049750:Genome, Insect D018257:Helix-Loop-Helix Motifs D019476:Insect Proteins D005810:Multigene Family D014863:Wasps
basic helix-loop-helix Nasonia vitripennis phylogenetic analysis BLAST search analyse phylogénétique interrogation BLAST motif basique hélice-boucle-hélice ortholog orthologue