Screening of Anti-Biofilm Compounds from Marine-Derived Fungi and the Effects of Secalonic Acid D on Staphylococcus aureus Biofilm.
Biofilm formation of Staphylococcus aureus is one of its mechanisms of drug resistance. Antibiofilm screening of 106 compounds from marine-derived fungi displayed that 12 compounds inhibited S. aureus biofilm formation by >50% at the concentration of 100 μg/ml, and only secalonic acid D (SAD) and B inhibited by >90% at 6.25 μg/ml without inhibiting cell growth after 24-h incubation. Meanwhile, it was found that the double bond between C-1 and C-10 of citrinin derivatives and the C-C connection position of two chromone monomers may be important for their anti-biofilm activities. Moreover, SAD slightly facilitated biofilm eradication and influenced its architecture. Furthermore, SAD slowed the cell growth rate in the preceding 18-h incubation and differentially regulated transcriptional expression of several genes, such as agr, isaA, icaA, and icaD, associated with biofilm formation in planktonic and biofilm cells, which may be the reason for the anti-biofilm activity of SAD. Finally, SAD acted synergistically against S. aureus growth and biofilm formation with other antibiotics. These findings indicated that various natural products from marine-derived fungi, such as SAD, could be used as a potential biofilm inhibitor against S. aureus.
['Wang|Jie|J|', 'Nong|Xu-Hua|XH|', 'Zhang|Xiao-Yong|XY|', 'Xu|Xin-Ya|XY|', 'Amin|Muhammad|M|', 'Qi|Shu-Hua|SH|']
D000900:Anti-Bacterial Agents D059001:Aquatic Organisms D001426:Bacterial Proteins D018441:Biofilms D001688:Biological Products D002953:Citrinin D005658:Fungi D015964:Gene Expression Regulation, Bacterial D006801:Humans D008826:Microbial Sensitivity Tests D013203:Staphylococcal Infections D013211:Staphylococcus aureus D044004:Xanthones
secalonic acid D Staphylococcus aureus Anti-biofilm activity marine natural product mechanism of action