Antioxidant response of cucumber (Cucumis sativus) exposed to nano copper pesticide: Quantitative determination via LC-MS/MS.
Targeted metabolomics aims to provide a new approach to investigate metabolites and gather both qualitative and quantitative information. We describe a protocol for extraction and analysis of plant metabolites, specifically 13 secondary metabolites (antioxidants) using liquid chromatography coupled to triple quadrupole mass spectrometry (LC-MS/MS), with high linearity (R2 > 0.99) and reproducibility (0.23-6.23 R%) with low limits of detection (>0.001 ng/mL) and quantification (>0.2 ng/mL). The protocol was applied to study the antioxidant response of cucumber plants exposed to nanocopper pesticide. Dose-dependent changes in antioxidant concentrations were found, and 10 antioxidants were significantly consumed to scavenge reactive oxygen species, protecting plants from damage. Levels of three antioxidants were up-regulated, as a response to the depletion of the other antioxidants, signaling activation of the defense system. We demonstrated that the reported LC-MS/MS method provides a quantitative analysis of antioxidants in plant tissues, for example to investigate interactions between plants and nanomaterials.
['Huang|Yuxiong|Y|', 'Adeleye|Adeyemi S|AS|', 'Zhao|Lijuan|L|', 'Minakova|Anastasiia S|AS|', 'Anumol|Tarun|T|', 'Keller|Arturo A|AA|']
D002853:Chromatography, Liquid D003300:Copper D018553:Cucumis sativus D010575:Pesticides D015203:Reproducibility of Results D053719:Tandem Mass Spectrometry
Antioxidants Cucumber LC-MS/MS Metabolites Extraction Nanoparticle