Brilliant blue G attenuates lipopolysaccharide-mediated microglial activation and inflammation.
Previous studies have confirmed that oxidized adenosine triphosphate, a P2X7 receptor antagonist, attenuates lipopolysaccharide-mediated microglial activation and inflammatory expression following neuronal damage in rat brain. NaCl and temperature may affect the potency of oxidized adenosine triphosphate. Brilliant blue G is a derivative of a widely used food additive and has little toxicity. This study explored the effects of brilliant blue G, a selective P2X7 receptor antagonist, on microglial activation and inflammation. Results demonstrated that brilliant blue G inhibited the release of cyclooxygenase-2 and interleukin-6 in BV2 cells. Immunofluorescence displayed that brilliant blue G could suppress lipopolysaccharide-induced microglial activation. This study used RNA interference to block P2X7 receptor expression and found that small interfering RNA also suppressed the release of cyclooxygenase-2 and interleukin-6 in BV2 cells. These results suggested that downregulation of the P2X7 receptor by brilliant blue G was involved in the inhibition of microglial activation and inflammation.
['Lu|Kui|K|', 'Wang|Jue|J|', 'Hu|Bin|B|', 'Shi|Xiaolei|X|', 'Zhou|Junyi|J|', 'Tang|Yamei|Y|', 'Peng|Ying|Y|']
brilliant blue G P2X7 receptor cyclooxygenase-2 interleukin-6 RNA interference grants-supported paper inflammatory cytokines lipopolysaccharide microglia neural regeneration neurodegenerative disease neuroregeneration photographs-containing paper