Isolation and identification of anti-proliferative peptides from Spirulina platensis using three-step hydrolysis.
BACKGROUND
Spirulina platensis is an excellent source of proteins (>60%) that can be hydrolyzed into bioactive peptides.


RESULTS
In this study, whole proteins of Spirulina platensis were extracted and hydrolyzed using three gastrointestinal endopeptidases (pepsin, trypsin and chymotrypsin). Subsequently, gel filtration chromatography was employed to separate hydrolysates, and four fractions (Tr1-Tr4) were obtained. Among them, Tr2 showed the strongest anti-proliferation activities on three cancer cells (MCF-7, HepG-2 and SGC-7901), with IC50 values of <31.25, 36.42 and 48.25 µg mL-1 , respectively. Furthermore, a new peptide, HVLSRAPR, was identified from fraction Tr1. This peptide exhibited strong inhibition on HT-29 cancer cells with an IC50 value of 99.88 µg mL-1 .


CONCLUSION
Taken together, these peptides possessed anti-proliferation activities on cancer cells and low cytotoxicity on normal cells, suggesting that they might serve as a natural anticancer agent for nutraceutical and pharmaceutical industries. © 2016 Society of Chemical Industry.
['Wang|Zhujun|Z|', 'Zhang|Xuewu|X|']
D020418:Algal Proteins D000595:Amino Acid Sequence D016588:Anticarcinogenic Agents D001426:Bacterial Proteins D002460:Cell Line D045744:Cell Line, Tumor D049109:Cell Proliferation D002681:China D002918:Chymotrypsin D003110:Colonic Neoplasms D019587:Dietary Supplements D055808:Drug Discovery D022781:Hepatocytes D006801:Humans D008970:Molecular Weight D009369:Neoplasms D009842:Oligopeptides D010434:Pepsin A D010446:Peptide Fragments D011492:Protein Hydrolysates D053545:Spirulina D014357:Trypsin
Spirulina platensis hydrolysis gastrointestinal endopeptidases anti-proliferation