CircZNF609/miR-134-5p/BTG-2 axis regulates proliferation and migration of glioma cell.
OBJECTIVES
MicroRNAs are abundant in eukaryotic cells and play key roles in cancers. Circular RNAs (CircRNAs) served as the competing endogenous RNAs (ceRNAs) in mediating multiple cell processes. This study aims to define the role of CircRNA CircZNF609/miR-134-5p in glioma as well as the underlying regulating mechanism.


METHODS
Relative expression of miR-134-5p, CircZNF609 and BTG-2 mRNA was determined by quantitative real-time PCR. Cell proliferation was analysed by CCK-8 assay. Cell migration was assessed by cell wound scratch assay. The direct regulatory of miR-134-5p on BTG-2 and CircZNF609 was verified by luciferase report gene assay.


KEY FINDINGS
MiR-134-5p was significantly upregulated in glioma cells. The overexpression of miR-134-5p inhibited cell proliferation and migration of glioma cell U251 and U87. Reversely, knock-down of miR-134-5p enhanced cell proliferation and migration. Both BTG-2 and CircZNF609 are the direct targets of miR-134-5p, and their expression could be negatively regulated by miR-134-5p. CircZNF609 was significantly upregulated in U251 and U87 cells and acted as an oncogene to promote cell proliferation and cell migration of glioma cell U251 and U87.


CONCLUSIONS
These data proved that CircZNF609 served as a competing RNA to bind miR-134-5p that promoted BTG-2 expression leading to reduced proliferation and migration of glioma cell.
['Tong|Hui|H|', 'Zhao|Kai|K|', 'Wang|Jiangjie|J|', 'Xu|Hui|H|https://orcid.org/0000-0002-0711-417X', 'Xiao|Jianqi|J|']
D001932:Brain Neoplasms D045744:Cell Line, Tumor D002465:Cell Movement D049109:Cell Proliferation D015972:Gene Expression Regulation, Neoplastic D005910:Glioma D006801:Humans D017874:Immediate-Early Proteins D035683:MicroRNAs D000079962:RNA, Circular D015398:Signal Transduction D025521:Tumor Suppressor Proteins
CircZNF609 miR-134-5p BTG-2 migration glioma