A Newly Discovered Phenylethanoid Glycoside from Stevia rebaudiana Bertoni Affects Insulin Secretion in Rat INS-1 Islet β Cells.
The tea-like beverage Stevia rebaudiana Bertoni (Stevia) is popular in China because it reduces blood glucose and has a sweet taste. In this work, a comprehensive quality assessment of Stevia led to the discovery of five phenylethanoid glycosides, namely steviophethanoside (1), cuchiloside (2), salidroside (3), icariside D (4), and tyrosol (5). Of them, compound 1 is a novel compound. Mass spectrometry and NMR spectroscopy were employed to confirm the absolute configuration. A hydrolytic step with 4 N TFA at 95 °C for 4 h was used to confirm the monosaccharides. In addition, Discovery Studio 4.0 was used to predict the ADME and toxicity activity of compound 1. The results suggested that compound 1 was biocompatible and had poor toxicity, which was verified by rat INS-1 islet β cells through an MTT assay. Meanwhile, a significant stimulatory effect on INS-1 cells was observed, which indicated a hypoglycemic effect of compound 1. This is the first report that describes a natural, novel, and hypoglycemic phenylethanoid glycoside in Stevia.
['He|Jing|J|', 'Zhu|Nai-Liang|NL|0000-0001-7488-1291', 'Kong|Jing|J|', 'Peng|Ping|P|', 'Li|Lin-Fu|LF|', 'Wei|Xiao-Lu|XL|', 'Jiang|Yan-Yan|YY|', 'Zhang|Yan-Ling|YL|', 'Bian|Bao-Lin|BL|', 'She|Gai-Mei|GM|0000-0003-1754-2140', 'Shi|Ren-Bing|RB|']
D000818:Animals D002478:Cells, Cultured D002681:China D006027:Glycosides D000078790:Insulin Secretion D050417:Insulin-Secreting Cells D009682:Magnetic Resonance Spectroscopy D013058:Mass Spectrometry D015394:Molecular Structure D010936:Plant Extracts D051381:Rats D036625:Stevia
phenylethanoid glycoside Stevia rebaudiana Bertoni insulin secretion DS structure