Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases.
Recombinase-mediated cassette exchange, or RMCE, is a genome engineering tool that can be used to swap DNA fragments of interest between two DNA molecules. In a variation of RMCE, called dual RMCE, the exchange of DNA fragments is mediated by two recombinases in contrast to one recombinase in the classic RMCE reaction. Under optimal conditions, the efficiency of dual RMCE can be quite high: up to ~45% of the transfected cells depending on the recombinase pair used to mediate the replacement reaction. Here we describe protocols for preparing for, performing, and optimizing the parameters of dual RMCE.
['Voziyanova|Eugenia|E|', 'Anderson|Rachelle P|RP|', 'Voziyanov|Yuri|Y|']
D004254:DNA Nucleotidyltransferases D018390:Gene Targeting D017930:Genes, Reporter D056426:Genetic Loci D005822:Genetic Vectors D049452:Green Fluorescent Proteins D057809:HEK293 Cells D006801:Humans D019426:Integrases D008164:Luminescent Proteins D016297:Mutagenesis, Site-Directed D011401:Promoter Regions, Genetic D011995:Recombination, Genetic D014162:Transfection D014443:Tyrosine
Dual recombinase-mediated cassette exchange Site-specific recombinases Cre Flp R TD