Histological, Physiological, and Comparative Proteomic Analyses Provide Insights into Leaf Rolling in Brassica napus.
Moderate leaf rolling is important in ideotype breeding, as it improves photosynthetic efficiency and therefore increases crop yields. To understand the regulatory network of leaf rolling in Brassica napus, a down-curved leaf mutant ( Bndcl1) has been investigated. Physiological analyses indicated that the chlorophyll contents and antioxidant enzyme activities were remarkably increased and the photosynthetic performance was significantly improved in Bndcl1. Consistent with these findings, 943 differentially accumulated proteins (DAPs) were identified in the Bndcl1 mutant and its wild-type plants using iTRAQ-based comparative proteomic analyses. Enrichment analysis of proteins with higher abundance in Bndcl1 revealed that the functional category "photosynthesis" was significantly overrepresented. Moreover, proteins associated with oxidative stress response and photosystem II repairing were also up-accumulated in Bndcl1, which might help the mutant to sustain the photosynthetic efficiency under unfavorable conditions. Histological observation showed that the mutant displayed defects in adaxial-abaxial patterning. Important DAPs associated with leaf polarity establishment were detected in Bndcl1, including ribosomal proteins, proteins involved in post-transcriptional gene silencing, and proteins related to brassinosteroid. Together, our findings may help clarify the mechanisms underlying leaf rolling and its physiological effects on plants and may facilitate ideotype breeding in Brassica napus.
['Chen|Wenjing|W|', 'Wan|Shubei|S|', 'Shen|Linkui|L|', 'Zhou|Ying|Y|', 'Huang|Chengwei|C|', 'Chu|Pu|P|0000-0003-1058-6263', 'Guan|Rongzhan|R|']
D000975:Antioxidants D029688:Brassica napus D002734:Chlorophyll D018506:Gene Expression Regulation, Plant D018384:Oxidative Stress D010788:Photosynthesis D045332:Photosystem II Protein Complex D018515:Plant Leaves D010940:Plant Proteins D040901:Proteomics
leaf rolling Brassica napus photosynthesis adaxial-abaxial polarity leaf proteome