Whole-genome sequencing to detect mutations associated with resistance to insecticides and Bt proteins in Spodoptera frugiperda.
The fall armyworm (FAW), Spodoptera frugiperda, is a major pest native to the Americas that has recently invaded the Old World. Point mutations in the target-site proteins acetylcholinesterase-1 (ace-1), voltage-gated sodium channel (VGSC) and ryanodine receptor (RyR) have been identified in S. frugiperda as major resistance mechanisms to organophosphate, pyrethroid and diamide insecticides respectively. Mutations in the adenosine triphosphate-binding cassette transporter C2 gene (ABCC2) have also been identified to confer resistance to Cry1F protein. In this study, we applied a whole-genome sequencing (WGS) approach to identify point mutations in the target-site genes in 150 FAW individuals collected from China, Malawi, Uganda and Brazil. This approach revealed three amino acid substitutions (A201S, G227A and F290V) of S. frugiperda ace-1, which are known to be associated with organophosphate resistance. The Brazilian population had all three ace-1 point mutations and the 227A allele (mean frequency = 0.54) was the most common. Populations from China, Malawi and Uganda harbored two of the three ace-1 point mutations (A201S and F290V) with the 290V allele (0.47-0.58) as the dominant allele. Point mutations in VGSC (T929I, L932F and L1014F) and RyR (I4790M and G4946E) were not detected in any of the 150 individuals. A novel 12-bp insertion mutation in exon 15 of the ABCC2 gene was identified in some of the Brazilian individuals but absent in the invasive populations. Our results not only demonstrate robustness of the WGS-based genomic approach for detection of resistance mutations, but also provide insights for improvement of resistance management tactics in S. frugiperda.
['Guan|Fang|F|', 'Zhang|Jianpeng|J|', 'Shen|Huiwen|H|', 'Wang|Xingliang|X|', 'Padovan|Amanda|A|', 'Walsh|Tom K|TK|', 'Tay|Wee Tek|WT|', 'Gordon|Karl H J|KHJ|', 'James|William|W|', 'Czepak|Cecilia|C|', 'Otim|Michael Hilary|MH|', 'Kachigamba|Donald|D|', 'Wu|Yidong|Y|https://orcid.org/0000-0003-3456-3373']
D000110:Acetylcholinesterase D000818:Animals D000083722:Bacillus thuringiensis Toxins D003958:Diamide D004731:Endotoxins D017344:Genes, Insect D049750:Genome, Insect D006460:Hemolysin Proteins D007305:Insecticide Resistance D007306:Insecticides D010755:Organophosphates D017354:Point Mutation D011722:Pyrethrins D019837:Ryanodine Receptor Calcium Release Channel D018411:Spodoptera D061566:Voltage-Gated Sodium Channels D000073336:Whole Genome Sequencing
whole-genome sequencing Spodoptera frugiperda Bt resistance insecticide resistance mutation detection