Genetic markers for antimony resistant clinical isolates differentiation from Indian Kala-azar.
Visceral Leishmaniasis or Kala-azar is caused by the protozoan parasites belonging to the Genus Leishmania. Once thought eradicated from the Indian subcontinent, the disease came back with drug resistance to almost all prevalent drugs. Molecular epidemiological studies revealed the polymorphic nature of the population of the main player of the disease, Leishmania donovani and involvement of other species (L. tropica) and other genus (Leptomonas) with the disease. This makes control measures almost futile. It also strongly demands the characterization of each and every isolate mandatory which is not done. In this background, the present study has been carried out to assess the genetic attributes of each clinical isolates (n=26) of KA and PKDL patients from India and Bangladesh. All the isolates were characterized through Restriction Fragment Length Polymorphism (RFLP) analysis to ascertain their species identity. 46.2% of the isolates were found to be Sodium Stibogluconate (SSG) resistant by amastigote-macrophage model. When the clinical isolates were subjected to Single Stranded Conformation Polymorphism (SSCP) of Internal Transcribed Spacer 1 (ITS1), Internal Transcribed Spacer 2 (ITS2) and some anonymous markers, the drug resistant Leishmania isolates of SSG can be distinguished from the sensitive isolates distinctly. This study showed for the first time, the genetic markers for SSG drug resistance of Indian Kala-azar clinical isolates.
['Khanra|Supriya|S|', 'Sarraf|Nibedeeta Rani|NR|', 'Das|Shantanabha|S|', 'Das|Anjan Kumar|AK|', 'Roy|Syamal|S|', 'Manna|Madhumita|M|']
D000965:Antimony D000981:Antiprotozoal Agents D001459:Bangladesh D001853:Bone Marrow D016000:Cluster Analysis D016054:DNA, Protozoan D004351:Drug Resistance D005786:Gene Expression Regulation D017125:Genes, Protozoan D005819:Genetic Markers D006801:Humans D007194:India D007893:Leishmania donovani D007898:Leishmaniasis, Visceral D008297:Male D008875:Middle Aged D008969:Molecular Sequence Data D010802:Phylogeny D012150:Polymorphism, Restriction Fragment Length D017422:Sequence Analysis, DNA
Genetic markers Indian kala-azar Drug resistance Leishmania donovani L. tropica Single stranded conformation polymorphism