Isolation and characterization of a novel, T7-like phage against Aeromonas veronii.
A virulent Aeromonas veronii biovar sobria and the corresponding novel, lytic bacteriophage (VTCCBPA5) were isolated from village pond water. The phage was found to belong to family Podoviridae. PCR analysis of major capsid protein gene confirmed its classification to T7-like genus. The protein profiling by SDS-PAGE indicated the major structural protein to be ~ 45 kDa. The phage (VTCCBPA5) is host specific and is stable over a range of pH (6-10) and temperatures (4-45 °C). On the basis of restriction endonuclease analysis combined with prediction mapping, it was observed to vary significantly from previously reported podophages of Aeromonas sp., viz. phiAS7 and Ahp1. The phylogenetic analysis on the basis of PCR-amplified segment of DNA polymerase gene of phage revealed it being an outgroup from podophages of Klebsiella sp. and Pseudomonas sp. though a small internal fragment (359 bp) showed the highest identity (77%) with Vibrio sp. phages. Thus, this is the first report of a novel Podoviridae phage against A. veronii. It expands the assemblage of podophages against Aeromonas sp. and BPA5 could be potentially useful in biocontrol of environmentally acquired Aeromonas veronii infections.
['Anand|Taruna|T|http://orcid.org/0000-0003-3267-2824', 'Bera|Bidhan Ch|BC|http://orcid.org/0000-0001-9134-0737', 'Virmani|Nitin|N|http://orcid.org/0000-0001-5575-3307', 'Vaid|Rajesh Kumar|RK|http://orcid.org/0000-0002-7554-5238', 'Vashisth|Medhavi|M|http://orcid.org/0000-0002-9896-1891', 'Tripathi|Bhupendra Nath|BN|http://orcid.org/0000-0001-8530-0608']
D000070079:Aeromonas veronii D004279:DNA, Viral D004591:Electrophoresis, Polyacrylamide Gel D006863:Hydrogen-Ion Concentration D050296:Microbial Viability D008970:Molecular Weight D010802:Phylogeny D020161:Physical Chromosome Mapping D017902:Podoviridae D016133:Polymerase Chain Reaction D013696:Temperature D014764:Viral Proteins D014871:Water Microbiology
Characterization Aeromonas veronii Podoviridae DNA polymerase gene TEM