Emulating human microcapillaries in a multi-organ-chip platform.
Current microfluidic chip-based tissue culture systems lack a capillary endothelial vessel system, which would enable perfusion with blood. We utilise spatial cell cultures to populate a perfused multi-organ-chip platform-a microfluidic device recently introduced for substance testing. Complete biological vascularization of such culture systems is vital to properly emulate physiological tissue behaviour. In this study, we incorporated a fibrin scaffold into the two-organ-chip design. Herein, adipose-derived stromal cells (ASCs) directed human umbilical vein endothelial cells (HUVECs) to organise into tube-like structures. The ASCs induced tube formation of HUVECs in static and dynamic conditions. The replacement of full medium enriched with growth factors and foetal calf serum with basal medium resulted in viable cells with similar gene expression profiles. We regard this as a prerequisite for studies with organ constructs that have a need for a different medium formulation. Furthermore, we here address stability issues of the fibrin gel and fibrin composition for optimal microvessel formation.
['Hasenberg|Tobias|T|', 'Mühleder|Severin|S|', 'Dotzler|Andrea|A|', 'Bauer|Sophie|S|', 'Labuda|Krystyna|K|', 'Holnthoner|Wolfgang|W|', 'Redl|Heinz|H|', 'Lauster|Roland|R|', 'Marx|Uwe|U|']
D000273:Adipose Tissue D002196:Capillaries D018929:Cell Culture Techniques D018920:Coculture Techniques D003470:Culture Media D005337:Fibrin D005782:Gels D049452:Green Fluorescent Proteins D061307:Human Umbilical Vein Endothelial Cells D006801:Humans D044085:Microfluidics D010477:Perfusion D016133:Polymerase Chain Reaction D012212:Rheology D013234:Stem Cells D013997:Time Factors D023822:Tissue Engineering D054457:Tissue Scaffolds D059467:Transcriptome
Multi-organ-chip Fibrin scaffold Co-culture models Tissue engineering Vasculature Vasculogenesis