Chromogenic sensing of biological thiols using squarylium dye.
A new highly selective probe for biothiols, squarylium dye (SQ), was designed and synthesized. The probe displayed a color change from blue to colorless upon reaction with biothilos such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH). The competition experiments revealed that no obvious interference was observed by performing the titration with the mixtures of Cys and other amino acids. The results indicated that SQ was highly selective for Cys detection. Moreover, SQ could also serve as a "naked-eye" probe for Cys with a minimum detectable concentration of approximately 4 μM.
['Shin|In-Sub|IS|', 'Gwon|Seon-Yeong|SY|', 'Kim|Sung-Hoon|SH|']
D004396:Coloring Agents D003545:Cysteine D005978:Glutathione D006710:Homocysteine D012680:Sensitivity and Specificity D013053:Spectrophotometry D013438:Sulfhydryl Compounds
Squarylium dye Biothiols Cysteine Homocysteine Glutathione Chemosensor