Intrathecal injection of adenosine 2A receptor agonists reversed neuropathic allodynia through protein kinase (PK)A/PKC signaling.
A single intrathecal dose of adenosine 2A receptor (A2AR) agonist was previously reported to produce a multi-week reversal of allodynia in a chronic constriction injury (CCI) model of neuropathic pain. We aimed to determine if this long-term reversal was induced by A2AR agonism versus more generalized across adenosine receptor subtypes, and begin to explore the intracellular signaling cascades involved. In addition, we sought to identify whether the enduring effect could be extended to other models of neuropathic pain. We tested an A1R and A2BR agonist in CCI and found the same long duration effect with A2BR but not A1R agonism. An A2AR agonist (ATL313) produced a significant long-duration reversal of mechanical allodynia induced by long established CCI (administered 6 weeks after surgery), spinal nerve ligation and sciatic inflammatory neuropathy. To determine if ATL313 had a direct effect on glia, ATL313 was coadministered with lipopolysaccharide to neonatal microglia and astrocytes in vitro. ATL313 significantly attenuated TNFα production in both microglia and astrocytes but had no effect on LPS induced IL-10. Protein kinase C significantly reversed the ATL313 effects on TNFα in vitro in microglia and astrocytes, while a protein kinase A inhibitor only effected microglia. Both intrathecal PKA and PKC inhibitors significantly reversed the effect of the A2AR agonist on neuropathic allodynia. Therefore, A2AR agonists administered IT remain an exciting novel target for the treatment of neuropathic pain.
['Loram|Lisa C|LC|', 'Taylor|Frederick R|FR|', 'Strand|Keith A|KA|', 'Harrison|Jacqueline A|JA|', 'Rzasalynn|Rachael|R|', 'Sholar|Paige|P|', 'Rieger|Jayson|J|', 'Maier|Steven F|SF|', 'Watkins|Linda R|LR|']
D058908:Adenosine A2 Receptor Agonists D000818:Animals D002478:Cells, Cultured D002908:Chronic Disease D003251:Constriction, Pathologic D017868:Cyclic AMP-Dependent Protein Kinases D006930:Hyperalgesia D007249:Inflammation D007278:Injections, Spinal D008026:Ligation D008297:Male D010880:Piperidines D011493:Protein Kinase C D011897:Random Allocation D051381:Rats D017207:Rats, Sprague-Dawley D020426:Sciatic Neuropathy D015398:Signal Transduction
Intrathecal Protein kinase ATL313 Mechanical allodynia Microglia Astrocytes Interleukin-10 Rats