Fluorescence properties of curcumin-loaded nanoparticles for cell tracking.
Background
Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems.


Materials and methods
In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done.


Results
Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking.


Conclusion
This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.
['Mogharbel|Bassam Felipe|BF|', 'Francisco|Julio Cesar|JC|', 'Irioda|Ana Carolina|AC|', 'Dziedzic|Dilcele Silva Moreira|DSM|', 'Ferreira|Priscila Elias|PE|', 'de Souza|Daiany|D|', 'de Souza|Carolina Maria Costa Oliveira|CMCO|', 'Neto|Nelson Bergonse|NB|', 'Guarita-Souza|Luiz Cesar|LC|', 'Franco|Celia Regina Cavichiolo|CRC|', 'Nakamura|Celso Vataru|CV|', 'Kaplum|Vanessa|V|', 'Mazzarino|Letícia|L|', 'Lemos-Senna|Elenara|E|', 'Borsali|Redouane|R|', 'Soto|Paula A|PA|', 'Setton-Avruj|Patricia|P|', 'Abdelwahid|Eltyeb|E|', 'de Carvalho|Katherine Athayde Teixeira|KAT|']
D000818:Animals D002454:Cell Differentiation D058948:Cell Tracking D002522:Chlorocebus aethiops D003474:Curcumin D005453:Fluorescence D049452:Green Fluorescent Proteins D016130:Immunophenotyping D045164:Mesenchymal Stem Cell Transplantation D059630:Mesenchymal Stem Cells D009203:Myocardial Infarction D053758:Nanoparticles D009409:Nerve Crush D017208:Rats, Wistar D012584:Sciatic Nerve D014709:Vero Cells
mesenchymal stem cells sciatic nerve crush transplantation cell marking myocardium infarction