Draft genome sequence of a CTX-M-8, CTX-M-55 and FosA3 co-producing Escherichia coli ST117/B2 isolated from an asymptomatic carrier.
OBJECTIVES
Asymptomatic carriers can act as reservoirs of multidrug-resistant (MDR) bacteria. The aim of this study was to describe the draft genome sequence of a MDR Escherichia coli lineage recovered from a faecal sample of a healthy carrier.


METHODS
Genomic DNA was sequenced on an Illumina NextSeq platform. Sequence reads were de novo assembled using CLC Genomics Workbench and the whole genome sequence was evaluated through bioinformatics tools available from the Center of Genomic Epidemiology as well as additional in silico analysis.


RESULTS
The genome size was calculated as 5178340 bp, with 5442 protein-coding sequences and 5492 total genes. Presence of the blaCTX-M-8, blaCTX-M-55 and fosA3 genes was detected in addition to other antimicrobial resistance genes. Interestingly, the strain was assigned to serotype O8:H4-fimH97 and was classified within the highly virulent phylogroup B2.


CONCLUSION
This draft genome can provide helpful information to elucidate genetic features that contribute to colonisation and adaptation of MDR and virulent pathogens in asymptomatic carriers.
['Fernandes|Miriam R|MR|', 'Sellera|Fábio P|FP|', 'Moura|Quézia|Q|', 'Souza|Tiago A|TA|', 'Lincopan|Nilton|N|']
D000900:Anti-Bacterial Agents D058070:Asymptomatic Diseases D001483:Base Sequence D002353:Carrier State D024901:Drug Resistance, Multiple, Bacterial D004926:Escherichia coli D004927:Escherichia coli Infections D029968:Escherichia coli Proteins D005260:Female D016680:Genome, Bacterial D006801:Humans D008826:Microbial Sensitivity Tests D008875:Middle Aged D009154:Mutation D001618:beta-Lactamases
Escherichia coli Virulent ESBL Extended-spectrum β-lactamase Human Whole-genome sequencing