Detection of pork adulteration by highly-specific PCR assay of mitochondrial D-loop.
We describe a highly specific PCR assay for the authentic identification of pork. Accurate detection of tissues derived from pig (Sus scrofa) was accomplished by using newly designed primers targeting porcine mitochondrial displacement (D-loop) region that yielded an unique amplicon of 712 base pairs (bp). Possibility of cross-amplification was precluded by testing as many as 24 animal species (mammals, birds, rodent and fish). Suitability of PCR assay was confirmed in raw (n = 20), cooked (60, 80 and 100 °C), autoclaved (121 °C) and micro-oven processed pork. Sensitivity of detection of pork in other species meat using unique pig-specific PCR was established to be at 0.1%; limit of detection (LOD) of pig DNA was 10 pg (pico grams). The technique can be used for the authentication of raw, processed and adulterated pork and products under the circumstances of food adulteration related disputes or forensic detection of origin of pig species.
['Karabasanavar|Nagappa S|NS|', 'Singh|S P|SP|', 'Kumar|Deepak|D|', 'Shebannavar|Sunil N|SN|']
D000818:Animals D017931:DNA Primers D004272:DNA, Mitochondrial D005506:Food Contamination D008460:Meat D016133:Polymerase Chain Reaction D034421:Sus scrofa
Pork Adulteration PCR Pig Primers DNA