Electrochemiluminescence (ECL) immunosensor for detection of Francisella tularensis on screen-printed gold electrode array.
An electrochemiluminescence (ECL) immunosensor for the rapid detection of the Francisella tularensis pathogen using whole antibodies or antibody fragments as capture biomolecule is described. A sandwich immunoassay was used with either lipopolysaccharide (LPS) or the whole inactivated bacterial cell (LVS) as a target, while Ru(bpy)3 (2+)-encapsulated silicate nanoparticles were linked to the secondary antibody and used as ECL labels. The assay was performed in a fluidic chip housed in a custom-built black box incorporating electronics, optics and fluidics. The obtained limit of detection for LPS was 0.4 ng/mL, while for the LVS it was 70 and 45 bacteria/mL when the capturing molecule was the whole antibody and the antibody F(ab) fragment, respectively.
['Spehar-Délèze|Anna-Maria|AM|', 'Julich|Sandra|S|', 'Gransee|Rainer|R|', 'Tomaso|Herbert|H|', 'Dulay|Samuel B|SB|', "O'Sullivan|Ciara K|CK|"]
D000907:Antibodies, Bacterial D055910:Antibodies, Immobilized D015374:Biosensing Techniques D055664:Electrochemical Techniques D004566:Electrodes D004867:Equipment Design D005604:Francisella tularensis D006046:Gold D006801:Humans D007118:Immunoassay D057230:Limit of Detection D008163:Luminescent Measurements D053758:Nanoparticles D009942:Organometallic Compounds D017640:Silicates D014406:Tularemia
Electrochemiluminescence Antibody fragment Immunoassay Biowarfare agent Screen-printed gold array