Penicillin G as a novel chiral selector in capillary electrophoresis.
The penicillin sub-class of β-lactam antibiotics has not been examined for its enantiodiscriminating abilities in capillary electrophoresis (CE) until date. The present work was therefore designed to evaluate penicillin G potassium salt (PenG) as an ion-pair chiral selector (CS) using CE for its several attributes, namely, high solubility in water and lower alcohols, structure allowing multiple interactions with analytes and cost-effectiveness. Systematic experiments were performed to investigate the effect of composition of background electrolyte, applied voltage and capillary temperature on chiral separation. Baseline resolutions of enantiomers of five basic chiral drugs (namely, darifenacin, citalopram, sertraline, propranolol and metoprolol) were attained using a background electrolyte composed of water:methanol (90:10, v/v) and consisting of 10.7 or 16.1mM CS at 20°C using an applied voltage of 5kV.
['Dixit|Shuchi|S|', 'Park|Jung Hag|JH|']
D000900:Anti-Bacterial Agents D001572:Benzofurans D015283:Citalopram D019075:Electrophoresis, Capillary D006863:Hydrogen-Ion Concentration D000432:Methanol D008790:Metoprolol D010400:Penicillin G D011433:Propranolol D011759:Pyrrolidines D020280:Sertraline D012995:Solubility D013237:Stereoisomerism D013696:Temperature
Penicillin G Penicillin G Chiral selector Chiral selector Capillary electrophoresis PenG CS darifenacin propranolol metoprolol methanol CIT DAR Enantioseparation METO MeCN MeOH PRO SERT acetonitrile chiral selector citalopram hydrobromide penicillin G sertraline hydrochloride