Immunochromatography test for rapid diagnosis of Mycoplasma pneumoniae infection.
The sensitivity and specificity of a new rapid Mycoplasma pneumoniae antigen immunochromatography (IC) test, DK-MP-001, were determined using particle agglutination (PA) antibody response and loop-mediated isothermal amplification (LAMP) gene detection as the gold standard. Of 165 patients, 59 were diagnosed with M. pneumoniae infection based on a ≥fourfold rise of serum PA antibody during the course of the illness. Of the first visit swabs, 60 were positive for M. pneumoniae on LAMP, and 49 were positive for M. pneumoniae antigen on IC test. Compared with PA antibody and LAMP, the sensitivity/specificity of the IC test were 81.4% (48/59) and 99.1% (105/106); and 81.7% (49/60) and 100% (105/105), respectively. IC test detected antigen in pharyngeal swabs more sensitively than in nasal swabs for the same subjects (P < 0.05). The IC test performs well enough to be used with pharyngeal swabs at the first examination.
['Onari|Shigeru|S|', 'Okada|Takashige|T|', 'Okada|Takafumi|T|', 'Okano|Syuko|S|', 'Kakuta|Osamu|O|', 'Kutsuma|Hirokazu|H|', 'Kobayashi|Masaaki|M|', 'Kondo|Yasuo|Y|', 'Sakaguchi|Naoya|N|', 'Tajima|Takeshi|T|', 'Nagao|Masayoshi|M|', 'Nakayama|Eiichi|E|', 'Niimi|Ryo|R|', 'Nishimura|Syuichi|S|', 'Higashidate|Yoshihito|Y|', 'Hikita|Toshiyuki|T|', 'Meguro|Hidenori|H|', 'Mori|Toshihiko|T|', 'Yoto|Yuko|Y|http://orcid.org/0000-0002-9728-8463', 'Tsutsumi|Hiroyuki|H|http://orcid.org/0000-0002-8674-0979']
D000293:Adolescent D000328:Adult D000368:Aged D002648:Child D002675:Child, Preschool D002846:Chromatography, Affinity D005260:Female D006801:Humans D007223:Infant D008297:Male D008875:Middle Aged D011019:Pneumonia, Mycoplasma D012189:Retrospective Studies D012680:Sensitivity and Specificity D055815:Young Adult
immunochromatography rapid diagnosis Mycoplasma pneumoniae Mycoplasma pneumoniae pneumonia