<sod>Native gels are run in non-denaturing conditions, so that the analyte's natural structure is maintained.  This allows the physical size of the folded or assembled complex to affect the mobility, allowing for analysis of all four levels of the biomolecular structure.  For biological samples, detergents are used only to the extent that they are necessary to [[lysis|lyse]] [[lipid bilayer|lipid membranes]] in the [[cell (biology)|cell]]. Complexes remain—for the most part—associated and folded as they would be in the cell. One downside, however, is that complexes may not separate cleanly or predictably, as it is difficult to predict how the molecule's shape and size will affect its mobility.<eod>
<sod>Native gels are run in non-denaturing conditions, so that the analyte's natural structure is maintained.  This allows the physical size of the folded or assembled complex to affect the mobility, allowing for analysis of all four levels of the biomolecular structure.  For biological samples, detergents are used only to the extent that they are necessary to [[lysis|lyse]] [[lipid bilayer|lipid membranes]] in the [[cell (biology)|cell]]. Complexes remain—for the most part—associated and folded as they would be in the cell. One downside, however, is that complexes may not separate cleanly or predictably, as it is difficult to predict how the molecule's shape and size will affect its mobility. Addressing and solving this problem is a major aim of [[QPNC-PAGE|quantitative native PAGE]]. <eod>