The major problem with obtaining clean blotting using RNA is the presence of formaldehyde. 흘러나온 젤을 증류수에 몇 분 동안 담가두었다가 증류수를 바꾸어 매트릭스에서 포름알데히드를 제거했습니다. The transfer assembly was assembled in exactly the same fashion as for DNA Southern blotting.