trxAxHisWDR M proteins purified from E. coli as described above were used as prey. 용해 완충액에 희석한 약 g의 먹이 단백질을 미끼 결합 비드와 함께 C에서 1분간 배양했습니다. Subsequently, beads were washed with lysis buffer four times and then subjected to SDSPAGE.