A primary antibody was then added to the solution and incubated at C for hours. 과산화물 접합 2차 항체 용액을 사용하여 멤브레인을 C에서 1시간 동안 배양했습니다. The membranes were then incubated with a chemiluminescent substrate Thermo and exposed to film Millipore IPVH.