wv lysis and P. falciparum pellets were sonicated in RIPA buffer mM Tris, pH., mM NaCl, mM MgCl, mM EDTA, mM dithiothreitol, M ATP,. 트리톤 X, 노니뎃 P, 글리세로인산염, NaF,. mM sodium orthovanadate, mM phenylmethylsulfonyl fluoride, mM benzamidine, and ComplexTM protease inhibitors.