Plates were then washed times with the washing buffer. 분석 희석액에 5나노그램의 바이오티닐화된 matKLK를 각 웰 L웰에 첨가하고 부드럽게 흔들면서 실온 RT에서 1시간 동안 배양했습니다. Plates were washed times and alkaline phosphataseconjugated streptavidin was added L per well.