Bound material was eluted with SDSPAGE sample loading buffer, resolved by SDSPAGE, and analyzed by Coomassie staining. 시험관 내 경쟁 분석 대장균 BL DE에서 MBPInp, GSTPex 및 HisPex 단백질을 발현시켰습니다. Cells were grown and induced and lysates prepared as above.