Indirect Immunofluorescence CHIKV strain S was grown on Vero cells at a multiplicity of infection of.. 세포를 24시간 후 슬라이드에 펼치고 공기 건조시킨 후 극저온 아세톤에 고정했습니다. Serum samples were incubated for h IgG or overnight IgM on fixed cells.