Factors Affecting on Adyuvant Properties of Chitosan Derivatives at Parenteral Administration of Inactivated Viral Vaccines
Aim. The investigation of factors affecting on adjuvant properties of chitosan derivatives at parenteral administration of inactivated viral vaccines. Materials and Methods. Vaxigrip («Sanofi Pasteur», France), Fluarix (GSK, Holland), Agripal («Novartis», Italy) and Grippol («Microgen», RF) were investigated. As adjuvants, we used chitosan derivatives, kindly provided All-Research and Technology Institute of the Biological industry. Experiments were conducted on mice CBA and also on nonpedigreed mice. ELISE and HI test were used for determination of immune response. Protective effect of immunization was appreciated during intranasal infection by virulent influenza virus. Results. Adjuvant properties of chitosan derivatives depended from size of summary positive charge of NH 2-groups and chemical properties of anions. Chitosan glutamate and reacetylated chitosan (chitin) have most impressive adjuvant properties. It was shown, that the contact of inactivated influenza vaccines with acid media (pH 5.0) before immunization assists visible decrease of HA -antibodies titer owing to conformation changes of hemagglutinin. In this connection adjuvant properties of glutamate chitosan were investigated at different values of pH. Data obtained shown, that glutamate chitosan has excellent adjuvant properties both pH 5.0 and 6.2. Conclusion. Chitosan derivatives have different adjuvant properties depend on chemical nature peculiarities. Glutamate chitosan and reacetilated chitosan (chitin) have more impressive adjuvant properties. Glutamate chitosan has excellent adjuvant properties at wide range of pH, including low values (5.0).