Obtaining of the Complex Enzyme Preparation with Enhanced Pectinase Activity Using a New Mutant Strain <i>T. reesei</i> Co-44
In order to obtain a complex enzyme preparation providing efficient hydrolysis of the main plant non-starch polysaccharides — cellulose, xylans, and pectin, studies were conducted to optimize a fermentation medium composition for submerged cultivation of a new mutant strain T. reesei Co-44 – a highly active producer of endo-carbohydrases. A concentrate of soy low molecular components was chosen as the inducer of the key carbohydrases biosynthesis by the strain. The concentrate provided the maximum increase in polygalacturonase activity – more than 8 times, and an increased level of endoglucanase and xylanase biosynthesis. After cultivation of T. reesei Co-44 under optimized conditions, a complex enzyme preparation Xylorizin K4 was obtained, its physicochemical properties were studied. Using electrophoresis and MALDI⎯TOF mass spectrometry, the presence of endopolygalacturonase (GH28) T.reesei in Xylorizin K4 was confirmed. The studies have shown the potential of Xylorizin K4 application in production of soy protein concentrates to eliminate the main soybean non-starch polysaccharides.