Association of the hypermethylation status of @entity983 XXXX suppressor gene with the risk of @entity0 among Kurdish population from Western Iran.

@entity5 @entity1 @entity983 @entity0
UNASSIGNED: @entity0 is the most common @entity5 with high morbidity and mortality among @entity1 worldwide. Aberrant hypermethylation in promoter regions of the @entity5 suppressor genes such as @entity983 gene is a key event in the progression and development of @entity0 . The aim of the present study was to evaluate an association between @entity983 gene methylation status with the risk of @entity0 in an Iranian population. We studied 255 individuals, including 103 @entity1 with @entity0 , 102 first-degree female relatives of @entity1 (mother, sister, or daughter of @entity1 ), and 50 healthy individuals as a control group. Genomic DNA was extracted from peripheral blood leukocytes, and the @entity983 promoter methylation status was detected using methylation-specific PCR (MSP) method with specific methylated and unmethylated primers. In some samples, direct DNA sequencing was used to confirm the results obtained by the MSP method. The frequency of @entity983 -methylated (MM) genotype was 6  % in the healthy control group, 23.3  % in relatives of @entity1 , and 41.7  % in @entity1 (   (2)   =   24.62, p   <   0.001). There were significant differences in the frequency of @entity983 -methylated genotype between healthy control compared to that in @entity1 (   (2)   =   15.1, p   <   0.001) and also compared to that in relatives of @entity1 (   (2)   =   6.9, p   =   0.009). In the presence of @entity983 MM genotype, there was a 3.1-fold susceptibility to @entity0 compared to the UU genotype (p   <   0.001). Also, in the presence of @entity983 M allele, the risk of @entity0 was 2.71-fold compared to the presence of U allele (p   <   0.001). Our findings indicated increased frequency of hypermethylation of @entity983 promoter in the studied @entity1 and their relatives that could be considered as one of the epigenetic factors affecting the risk of @entity0 in Iranians.
