The mechanism of XXXX loading in improving postischemic myocardial recovery.

@entity1522 @entity2642 @entity63 @entity6546 @entity535 @entity26 @entity2036 @entity95 @entity1391 @entity6626 @entity880 @entity1562 @entity1718 @entity413
During induced @entity63 for cardiac surgery, @entity1562 occurs and aerobic metabolism of @entity413 , @entity1718 , and @entity880 is altered. Following reperfusion, stunned myocardium uses @entity26 and substrate inefficiently, leading to poor functional recovery. However, @entity1522 may be used as anaplerotic metabolic substrates during and after @entity63 , utilizing transamination of @entity1522 to form high-energy @entity6546 via the @entity6626 cycle. We investigated if loading hearts with a physiologic spectrum of @entity1522 prior to @entity63 could increase postischemic myocardial recovery. Isolated perfused @entity95 hearts were subjected to 120 min of 34 degrees C @entity63 . Hearts received cardioplegia alone as controls or were loaded with a 0.05, 0.1, 0.5, 1, 2, or 5% @entity535 perfusion prior to @entity63 . Following reperfusion, functional recovery revealed that hearts perfused with 0.05 and 0.1% @entity1522 had improved contractility and compliance vs untreated controls. To determine if the mechanism of @entity535 loading in improving postischemic function was enhancement of high-energy @entity1391 resynthesis, @entity2036 and @entity2642 were measured. While all preischemic values were equivalent, @entity535 -loaded hearts had significantly greater high energy nucleotides at end @entity63 and after reperfusion. These data demonstrate that metabolism, as well as function, is improved with @entity535 loading prior to @entity63 , which allowed for better internal reparative work during @entity63 and external contractile work after @entity63 . This strategy may have application in cardiac surgery.
