XXXX induce tight junctions to form in brain capillary endothelial cells.

@entity7784 @entity72317 @entity670 @entity11177 @entity24023 @entity1474 @entity2306 @entity11616 @entity18943 @entity669 @entity7461 @entity4722
Tight junctions create a rate-limiting barrier to the diffusion of solutes between vertebrate epithelial cells and endothelial cells. They are also controlled within individual cells by a variety of physiologically relevant signals. We investigated the effects of @entity1474 on the formation of tight junctions in brain capillary endothelial cells, monitoring the transepithelial electrical resistance, and analyzed the expression of @entity11177 messenger RNA. Brain-capillary endothelial cells were grown to confluence on filters and exposed to @entity72317 , @entity18943 and @entity4722 . Transepithelial electrical resistance was determined with voltage-measuring electrodes. The messenger RNA expression of @entity11177 was quantitated by real-time quantitative reverse transcriptase-polymerase chain reaction. The basal resistance across monolayers of porcine brain capillary endothelial cells was 83+/-8.1 Omega cm(2). Cells cultured in @entity72317 and @entity18943 , but not @entity7461 , displayed a 2.7-fold increase in transepithelial electrical resistance at 10 microM in brain capillary endothelial cells. The expression level of @entity11177 messenger RNA increased markedly immediately after the exposure to @entity72317 or @entity18943 . Following an 8 h exposure to exogenous @entity72317 or @entity18943 , @entity11177 messenger RNA levels were significantly increased. In addition, the rise in transepithelial electrical resistance induced by @entity72317 and @entity18943 was markedly inhibited by the @entity669 kinase inhibitors @entity670 and @entity24023 and protein kinase C inhibitor, @entity11616 . In contrast, the rise in transepithelial electrical resistance induced by @entity72317 and @entity18943 was not inhibited by the PI 3-kinase inhibitor, @entity2306 . We conclude that @entity72317 and @entity18943 increased the transepithelial electrical resistance and the expression of @entity11177 messenger RNA in brain capillary endothelial cells. This @entity18943 and @entity7784 induced assembly of tight junction is likely to be regulated by protein kinase C and @entity669 kinase activity.
