XXXX Promotes Ovarian Granulosa Cells Proliferation Through Targeting @entity994 in Polycystic Ovarian Syndrome.

@entity1 @entity2690 @entity2670 @entity6956 @entity994
CONTEXT: MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression post-transcriptionally. Whether differently expressed miRNAs contribute to promoting granulosa cell proliferation in @entity2670 ( @entity2670 ) remains unknown. OBJECTIVE: We explored whether certain miRNAs are involved in the @entity2670 of @entity2670 and the mechanism of increased granulosa cells proliferation. @entity1 and Cells: miRNA expression was analyzed in excised ovarian cortexes from 16 @entity1 with @entity2670 and 8 non- @entity2670 . An immortalized @entity1 granulosa (KGN) cell was used for the mechanism study. MAIN OUTCOME MEASURES: Expressions of miRNAs in ovarian cortexes were measured using qRT-PCR and KGN granulosa cells were cultured for proliferation assays after overexpression or inhibition of @entity6956 or after @entity2690 treatment. Bioinformatics were used to identify the potential miRNA targets. Protein expression analysis, luciferase assays, and rescue assays were used to confirm the substrate of @entity6956 . RESULTS: @entity6956 expression was higher in @entity2670 ovarian cortex and its identified target, @entity994 , was downregulated. @entity6956 overexpression promoted cell proliferation and G1 to S transition. Knocking down @entity994 promoted cell growth and cell cycle progression in granulosa cells, and @entity994 re-introduction reversed the promotional role of @entity6956 . High concentrations of @entity2690 induced upregulation of @entity6956 , stimulated KGN cells proliferation and reduced @entity994 expression. CONCLUSIONS: @entity6956 was increased in @entity2670 granulosa cells and targeted @entity994 to promote proliferation and cell cycle progression. @entity2690 could upregulate the expression of @entity6956 and stimulate cell proliferation. This might provide a new insight into the dysfunction of granulosa cells in @entity2670 .
