Strain-dependent variations in visceral sensitivity: relationship to stress, XXXX and spinal glutamate transporter expression.

@entity9140 @entity308 @entity25842 @entity19 @entity3825 @entity2819 @entity148 @entity56 @entity74 @entity158 @entity3689 @entity1744 @entity19472
UNASSIGNED: Responses to painful stimuli differ between populations, ethnic groups, sexes and even among individuals of a family. However, data regarding @entity3825 are still lacking. Thus, we investigated differences in visceral nociception across inbred and outbred @entity19 strains using colorectal distension. @entity148 and @entity308 -like behaviour were assessed using the open field and forced swim test as well as the @entity2819 stress response. Possible mechanistic targets [excitatory @entity3689 transporter ( @entity25842 ), @entity9140 ( @entity9140 ) and @entity19472 ] were also assessed using quantitative real-time polymerase chain reaction. Adult, male, inbred and outbred @entity19 strains were used in all assays (inbred strains; CBA/J Hsd, C3H/HeNHsd, BALB/c OlaHsd, C57 BL/6JOlaHsd, DBA/2J RccHsd, CAST/EiJ, SM/J, A/J OlaHsd, 129P2/OlaHsd, FVB/NHan Hsd and outbred strains: Swiss Webster, CD-1). mRNA expression levels of @entity25842 , @entity9140 and @entity19472 ( @entity19472 ) were quantified in the lumbosacral spinal cord, amygdala and hippocampus. A significant effect of strain was found in visceral sensitivity, @entity148 and @entity308 -like behaviours. Strain differences were also seen in both baseline and stress-induced @entity2819 levels. CBA/J @entity19 consistently exhibited heightened visceral sensitivity, @entity148 and @entity308 -like behaviour which were associated with decreased spinal @entity25842 and hippocampal @entity9140 and @entity19472 . Our results show the CBA/J @entity19 as a novel @entity19 model to unravel the complex mechanisms of brain-gut @entity74 such as @entity1744 , in particular the underlying mechanisms of @entity56 , for which there is great need. Furthermore, this study highlights the importance of genotype and the consequences for future development of transgenic strains in @entity158 research.
