Subcellular and subsynaptic localization of presynaptic and postsynaptic XXXX receptor subunits in the monkey striatum.

@entity2227 @entity2033 @entity4176 @entity14799 @entity19942 @entity2373
The localization and functions of @entity4176 receptors (KARs) in the CNS are still poorly known. In the striatum, @entity2373 /7 and @entity19942 immunoreactivity is expressed presynaptically in a subpopulation of glutamatergic terminals and postsynaptically in dendrites and spines. The goal of this study was to further characterize the subcellular and subsynaptic localization of @entity4176 receptor subunits in the monkey striatum. Immunoperoxidase data reveal that the relative abundance of @entity2373 /7- and @entity19942 -immunoreactive terminals is homogeneous throughout the striatum irrespective of the differential degree of @entity14799 in @entity2033 . Pre-embedding and post-embedding immunogold data indicate that >70% of the presynaptic or postsynaptic @entity2373 /7 and @entity19942 labeling is expressed intracellularly. In material stained with the post-embedding immunogold method, approximately one-third of plasma membrane-bound gold particles labeling in axon terminals and spines is associated with asymmetric synapses, thereby representing synaptic @entity4176 receptor subunits. On the other hand, >60% of the plasma-membrane bound labeling is extrasynaptic. Both @entity2373 /7 and @entity19942 labeling in glutamatergic terminals often occurs in clusters of gold particles along the membrane of large vesicular organelles located at various distances from the presynaptic grid. Anterograde labeling from the primary motor cortex or the centromedian thalamic nucleus indicate that both corticostriatal and thalamostriatal terminals express presynaptic @entity2373 /7 and @entity19942 immunoreactivity in the postcommissural putamen. In conclusion, these data demonstrate that @entity4176 receptors in the striatum display a pattern of subcellular distribution different from other ionotropic @entity2227 receptor subtypes, but consistent with their metabotropic-like functions recently shown in the hippocampus.
