Conjunctival structural and functional reconstruction using acellular XXXX pericardium graft (Normal GEN  ) in @entity95 .

@entity154 @entity1711 @entity95 @entity1710 @entity291
PURPOSE: To evaluate the effectiveness of acellular @entity291 pericardium grafts (Normal GEN  ) used as scaffolds for conjunctival reconstruction. METHOD: The acellular @entity291 pericardium graft and the amnion graft were implanted into the bulbar conjunctival defects of adult @entity95 . Conjunctival samples of implanted materials and @entity154 controls were observed at day 3, 7, 14, 21, 28, and 56 postoperatively. Histological examination was observed at day 14, 28, and 56 of surgery, including @entity1710 - @entity1711 staining, periodic acid-Schiff staining, and Masson's trichrome staining, while immunofluorescent microscopy was observed at 14  days and 28  days after surgery. Results were compared among the Normal GEN  , amnion, and @entity154 controls. RESULTS: All three groups showed complete conjunctival reconstruction. Wounds that were not grafted closed by formation of conjunctival scar characterized by a linear array of densely packed collagen fibers in Tenon's capsule. Subepithelial tissue in the grafted groups comprised a loosely organized network of randomly oriented collagen that resembled that of the normal bulbar conjunctiva. However, there was a dense layer of aligned collagen between the conjunctival Tenon's capsule and the sclera in the NormalGEN   group, about 250   m in thickness. CONCLUSIONS: Implantation of the NormalGEN   graft promoted the formation of conjunctiva as a kind of scaffold both in structure and in function. It had more advantageous mechanical properties than the amnion, strong and elastic, during the period of conjunctival reconstruction.
