Effect of XXXX on the morphology and growth of C3H/10T1/2 @entity19 embryo cells.

@entity5 @entity19 @entity10757 @entity4621 @entity865 @entity864
The effects of the @entity5 -promoting @entity4621 @entity864 ( @entity865 ) on the morphology and growth properties of @entity19 cells were examined. The morphology of these cells was changed within 30 min following treatment with 0.1 microgram of @entity865 per ml; they became smaller and refractile with long beady processes. Such changes were observed in both logarithmic and confluent cultures and lasted about 72 hr. Subsequent treatments were much less effective in inducing these changes. Scanning electron microscopy showed cell retraction and rounding as the most significant immediate effects of @entity865 treatment; many cells remained partially rounded 48 hr afterwards. Long-term surface modifications ascribable to @entity865 treatment were not detected. @entity865 had only minor effects on the growth of cultured C3H/10T1/2 cells in the presence of 10% fetal calf serum. Slight increases in plating efficiencies and saturation densities were generally observed in the presence of @entity865 but not with the related non- @entity5 -promoting compound @entity10757 . The cells grew slowly in 1% fetal calf serum and demonstrated serum batch-dependent alterations in their growth properties when exposed to @entity865 . Under conditions that produce doubling times of 70 hr or greater, @entity865 , but not @entity10757 , reduced the doubling time to about 50 hr. Saturation densities were also increased by @entity865 in 1% fetal calf serum. The effects of @entity865 on the growth of an oncogenically transformed variant of C3H/10T1/2 were quite different. While minimal effects of @entity865 were observed quite different. While minimal effects of @entity865 were observed when transformed cells were treated in the presence of 10% total calf serum, @entity865 treatment in 1% fetal calf serum significantly inhibited cell growth.
