New control elements of XXXX pre-replicative transcription.

@entity526 @entity72569 @entity1519 @entity42715 @entity21224
@entity1519 pre-replicative genes are transcribed, by @entity526 RNA polymerase, in two alternative modes: an early mode and a middle mode. Middle mode transcription is under the control of at least one viral protein, pmotA. We have identified two additional viral genes, @entity72569 and motC, that map in the dispensable region of the @entity1519 genome, between genes 39 and 56. pmotB and pmotC are diffusible factors which provide an alternative to the @entity21224 dependent mode of middle transcription of many @entity1519 genes. Deletions of @entity72569 and motC are in fact lethal only in combination with a @entity21224 mutant. @entity72569 controls one of the alternative modes of transcription of the @entity42715 gene. When @entity21224 or @entity72569 are missing, transcription of @entity42715 is quantitatively unaffected; when both are missing the transcription rate drops by about 75%. Control of transcription of the tRNA gene cluster is more complex. Transcription of subcluster 2 is maximally reduced (70%) only by deletions that, besides @entity72569 , cut out an adjacent region. We guess that this adjacent region codes for an additional control element, which we call motC. The @entity72569 gene is situated in a 750-base region between the left end-points of del(39-56)-1 and -4.
