Regulation of interacting populations during endocytosis: models of growth factor- XXXX promoter dynamics.

@entity558 @entity5 @entity4621 @entity4179 @entity4996
A model of growth factor-cell receptor interactions, including internalization, sorting, recycling, and degradation and their modulation by @entity5 promoters, is developed, analyzed, and tested. In keeping with data and concepts based on a large number of systems, the main assumption is that after receptor-ligand binding the complex associates with a second membrane protein, localized in @entity558 , and that this event is a necessary condition for receptor-mediated endocytosis and subsequent intracellular processes. As a consequence of the model, ligands having distinct receptors interfere at the cell surface through competition between their receptor complexes for a limited pool of coated pit proteins. The utility of the model is illustrated by a detailed analysis of binding, endocytosis, and degradation of @entity4179 ( @entity4179 ) and their modulation by @entity4996 . The analysis permits quantitative characterization of the dynamics of the endocytic processes and leads to the following conclusions. The Scatchard plot changes from linear to nonlinear as the ratio of the number of coated pit proteins to the number of receptors decreases. Competition between @entity4621 and @entity4179 -bound receptors for coated pit proteins predicts, in agreement with observation, conversion of nonlinear @entity4179 Scatchard plots to linear plots subsequent to reincubation with @entity4996 . The postulated competition suggests a local homology between the @entity4621 receptor and the @entity4179 receptor. Homologous and heterologous downregulations observed in numerous systems are natural consequences of the model. Preincubation with the heterologous ligand increases the time lag between ligand binding and lysosomal degradation and alters intracellular sorting.
