A novel primer set for improved direct gene sequencing of XXXX genotype-1 from clinical samples.

@entity1 @entity3548 @entity11332 @entity281 @entity1729 @entity580
UNASSIGNED: @entity11332 genotype (HBoV-1) is a parvovirus associated with @entity1729 in @entity1 with different degrees of severity. The current study intended to improve the direct gene sequencing of the HBoV-1 using a newly developed primer set. Screening the presence of @entity11332 @entity281 among in- @entity1 @entity1 suffering from @entity1729 was another aim of the current study. Nasopharyngeal swab samples from in- @entity1 @entity1 suffering from @entity1729 were examined. The real-time polymerase chain reaction was used for the initial screening as a highly sensitive method to detect the HBoV. Genotyping of real-time positive samples was attempted by direct sequencing of PCR amplicons using NP, VP1/2 and the newly developed @entity3548 /NC primers. HBoV-1 was present in 56.8% of the examined @entity1 . The newly developed primer set successfully amplified all real-time PCR positive samples, however, the other primer pairs did not reliably detect real-time PCR positive samples. The gene sequences of the detected HBoV-1 showed conserved sequences to each other with a low rate of discrepancies. The high rate of @entity281 and the similarity between the detected strains strongly suggest @entity580 .
