[The expression and effects of isoforms of macrophage colony stimulating factor in XXXX leukemic cell lines].

@entity6824 @entity1 @entity8990 @entity2198
OBJECTIVE: To explore the expression and effects of isoforms of @entity6824 ( @entity6824 ) in @entity1 leukemic cell lines. METHODS: Three normal @entity1 peripheral blood mononuclear cells (PBMCs) and 4 @entity1 myelomonocytic leukemic cell lines including J6-1, J6-2, K562 and HL-60 were studied using ABC immunoperoxidase assay, indirect immunofluorescence staining, flow cytometry, Western blot and reverse enzyme-linked DNA-protein interaction assay (reverse ELDIA). RESULTS: @entity6824 was noticed to be localized in the cytoplasm, nucleus and at the cell membrane in 4 @entity1 leukemic cell lines; expression of @entity6824 was not detected in normal @entity1 PBMCs without @entity8990 stimulation. @entity1 PBMCs stimulated by @entity8990 expressed a low level of @entity6824 . Frequencies of membrane bound @entity6824 expression in J6-1, J6-2, K562 and HL-60 were 71.6%, 69.7%, 42.7% and 57.4% respectively. Frequencies of cytoplasm and nucleus associated @entity6824 were 65.7%, 45.4%, 36.5% and 72.5% respectively. The cytosolic bound @entity6824 was expressed in J6-1 cell as four isoforms with a molecular @entity2198 . While nucleus associated @entity6824 expressed as two isoforms with a molecular weight of 16,000 and 20,000. Anti- @entity6824 monoclonal antibody could dramatically inhibit proliferation of leukemic cells and its inhibitory effect was related to the levels of membrane bound @entity6824 expression in leukemic cells. Reverse ELDIA showed that @entity6824 could bind with DNA in vitro. CONCLUSIONS: Expression of @entity6824 isoforms is heterogeneous and polymorphous in leukemic cells. Membrane bound @entity6824 is crucial for the proliferation of leukemic cells, which might be a DNA-bound protein and could be involved in the transformation and tumorigenesis of hematopoietic cells.
