@entity56087 affinity chromatography and XXXX colorimetry compared for measuring glycated albumin.

@entity413 @entity6 @entity4466 @entity56087
Two techniques originally developed for measurement of glycated ("glycosylated") hemoglobin but also applicable to determination of glycated albumin are the @entity4466 colorimetric technique (I) and the @entity56087 affinity chromatographic procedure (II). The latter reliably distinguishes @entity6 from nondiabetics, and concentrations of glycated hemoglobin and glycated albumin are linearly correlated. I is nonspecific; it neither correlates with @entity6 status nor with values derived via the affinity technique. Most of the chromogenic material is present in the fraction of albumin that does not bind to @entity56087 . @entity413 interferes significantly with I but only slightly with II. Prolonged incubation of plasma with @entity413 dramatically increases the II-determined glycated albumin. Reactivity with @entity4466 increases much less, and mainly in the II-bound fraction. This fraction contains a high proportion of nonspecifically reactive material. The percentage of glycated albumin determined in crude plasma samples by II differs only slightly from the value determined by purifying the albumin from the plasma. This technique appears more promising than I for eventual clinical applications.
