Establishment of a dual-color fluorescence tracing orthotopic transplantation model of XXXX .

@entity1 @entity5 @entity19 @entity3 @entity157 @entity7351
@entity157 ( @entity157 ) have been used to investigate the biological mechanisms of hepatocarcinogenesis and its progression. However, previous studies have highlighted the difficulty of distinguishing between the @entity5 cells and stroma in experimental models of @entity157 . Therefore the aim of the present study was to establish a red  -green dual  -color fluorescence tracing orthotopic transplantation model of @entity157 , and investigate its practical values. Stable high @entity7351 ( @entity7351 )  -expressing HepG2 @entity1 @entity157 cells and Hepa1  -6 @entity19 @entity157 cells were injected into the right liver lobe of green fluorescent protein  -expressing @entity19 . The growth and @entity3 of the @entity5 were visualized using a whole  -body in vivo fluorescence imaging system in real time. @entity157 tissues were extracted from @entity5  -bearing @entity19 , and cut into 5  -  m serial frozen slices. The organizational structure of the transplanted @entity5 was observed under a microscope. A dual  -color fluorescence tracing orthotopic transplantation @entity5 model of @entity157 was successfully established with a success rate of 100%. The growth and @entity3 of the @entity5 were visualized at each stage of development in the @entity5  -bearing @entity19 . @entity5 cells with red fluorescence and host cells with green fluorescence were identified to merge in the reconstruction region of @entity5 tissue. The invasion, migration, and cell fusion between @entity5 and host cells was observed clearly. The dual  -color fluorescence tracing orthotopic transplantation model of @entity157 was determined to be a stable and reliable method for tracking @entity5 progression. Mutual interactions between @entity157 cells and host tissues may be observed directly using this model, further elucidating the development of the @entity5 microenvironment.
