Morphological response of injured adult XXXX optic nerve to implants containing media conditioned by growing optic nerves.

@entity558 @entity851 @entity95 @entity18489 @entity1346
Adult @entity95 retina can express regeneration-associated characteristics after @entity18489 , provided it is supplied with appropriate diffusible substances originating from media conditioned by regenerating fish optic nerves or by optic nerves of a newborn @entity95 [Hadani et al., Proc. Natl. Acad. Sci. U.S.A., 81 (1984) 7965; Schwartz et al., Science, 228 (1985) 600]. This was shown by applying the active substances to the injured axons in the form of 'wrap-around' implants, consisting of collagen-coated @entity1346 tubes which had been soaked in the @entity558 ( @entity558 ). The regeneration-associated response was manifested biochemically and by sprouting of nerve fibers in culture. The present work provides morphological evidence that the implantation prolongs survival of ganglion cells and optic nerve fibers and induces @entity851 . Light microscopic analysis (using horseradish peroxidase (HRP) for labeling the fibers) revealed, 1 week following @entity18489 , labeled fibers and ganglion cells in both the implanted and control (injured only or injured and implanted with collagen-coated @entity1346 tubes free of @entity558 ) nerves. However, from the second week after the injury, distinct differences in the appearance of viable ganglion cells and labeled fibers, were seen between experimental and control preparations. In sections taken through the optic nerve, at the region distal to the site of injury, HRP-labeled fibers were seen in the experimental nerves 1 week, 2 weeks and to a significantly lesser extent 1 month after injury.(ABSTRACT TRUNCATED AT 250 WORDS)
