Dendritic cell-based vaccination combined with XXXX increases survival in a @entity19 @entity3123 model.

@entity1 @entity3123 @entity19 @entity5 @entity189 @entity204 @entity136 @entity9384
BACKGROUND: Tumour-specific cytotoxic T lymphocytes (CTLs) can be activated in vivo by vaccination with dendritic cells (DCs). However, clinical responses to DC-based vaccination have only been observed in a minority of @entity1 with solid @entity5 . Combination with other treatment modalities such as chemotherapy may overcome immunoresistance of @entity5 cells. It has been shown previously that @entity136 sensitises @entity1 @entity3123 cells against CTL-mediated lysis. Here, a @entity19 @entity3123 model was used to investigate whether combination with @entity136 increases therapeutic efficacy of DC-based vaccination. METHODS: Bone marrow-derived DCs from C57BL/6 @entity19 were loaded with UV-irradiated, syngeneic Panc02 @entity189 cells and were administered subcutaneously. For prophylactic vaccination, @entity19 were vaccinated three times at weekly intervals prior to @entity5 challenge with Panc02 cells. Therapeutic vaccination was started when @entity5 formed a palpable @entity9384 . @entity136 was administered intraperitoneally twice weekly. RESULTS: Prophylactic DC-based vaccination completely prevented @entity5 development and induced immunological memory as well as @entity5 antigen-specific CTLs. In the subcutaneous @entity5 model, therapeutic DC-based vaccination was equally effective as @entity136 (14% vs 17% survival at day 58 after @entity5 challenge; controls, 0%). Combination of the two strategies significantly increased survival of @entity5 -bearing @entity19 (50% at day 58 after @entity5 challenge). DC-based vaccination also prevented @entity204 from pulmonary metastatisation after intravenous injection of Panc02 cells. CONCLUSION: DC-based immunotherapy may not only be successfully combined with @entity136 for the treatment of advanced @entity3123 , but may also be effective in preventing local recurrence or metastatisation in @entity5 -free @entity1 .
