Transfer of spleen cells expanded by XXXX suppresses arthritis induced in @entity35 .

@entity35 @entity1573 @entity5041 @entity1753
The effects of transfer of @entity5041 ( @entity5041 )-expanded spleen cells after concanavalin A (Con A) stimulation into syngeneic Lewis @entity35 were studied. The recipient @entity35 were immunized with complete Freund's adjuvant for induction of @entity1753 ( @entity1753 ) or @entity1573 type II collagen in incomplete Freund's adjuvant for induction of @entity1753 ( @entity1753 ) on day 0. Each of 5 X 10(7) cultured cells without mitogenic stimulation, 2 X 10(7) Con A-stimulated cells, or 1 X 10(7) @entity5041 -expanded cells cultured for 8 days (4 days X 2 culture cycles) after Con A stimulation was given on days 0 and 7. Both transfers of the cultured cells without stimulation and @entity5041 -expanded cells markedly diminished the severity of @entity1753 and @entity1753 . On the contrary, transfer of Con A-stimulated cells led to no suppressive activity. In addition, transfer to @entity5041 -expanded cells significantly lowered the titre of anti-type II collagen antibody compared to that of control @entity35 . The transfer of 1 X 10(7) @entity5041 -expanded cells was optimal for suppressing @entity1753 , in terms of cell number. This observation suggests that these cells were much more effective than were the unstimulated cultured cells, for which more than five times the number was required for the same suppressive activity. As far as the phenotypic proportion of helper (W3/13) and suppressor (OX-8) cells is concerned, we found no significant differences between the cultured cell groups and the freshly separated spleen cell group. The precise mechanism of these suppressive effects is the subject of further study. The transfer of @entity5041 -expanded cells appears to have a potent immunomodulatory effect.
