Metabotropic @entity3264 receptors: modulators of context-dependent feeding behaviour in XXXX .

@entity1 @entity2199 @entity3264 @entity72165 @entity72163 @entity72164
UNASSIGNED: Glutamatergic neurotransmission is evolutionarily conserved across animal phyla. A major class of @entity3264 receptors are the metabotropic @entity3264 receptors (mGluRs). In @entity2199 three mGluR genes @entity72163 , @entity72164 and @entity72165 are organised into three sub-groups, similar to their @entity1 counterparts. Cellular reporters identified expression of the mgls in the nervous system of @entity2199 and overlapping expression in the pharyngeal microcircuit that controls pharyngeal muscle activity and feeding behaviour. The overlapping expression of mgls within this circuit allowed investigation of receptor signalling per se and in the context of receptor interactions within a neural network that regulates feeding. We utilized the pharmacological manipulation of neuronally regulated pumping of the pharyngeal muscle in wild type and mutants to investigate mgl function. This defined a net @entity72163 dependent inhibition of pharyngeal pumping which is modulated by @entity72165 excitation. Optogenetic activation of the pharyngeal glutamatergic inputs combined with electrophysiological recordings from the isolated pharyngeal preparations provided further evidence for a presynaptic @entity72163 dependent regulation of pharyngeal activity. Analysis of @entity72163 , @entity72164 and @entity72165 mutant feeding behaviour in the intact organism after acute food removal identified a significant role for @entity72163 in the regulation of an adaptive feeding response. Our data describes the molecular and cellular organisation of @entity72163 , @entity72164 and @entity72165 . Pharmacological analysis identified that in these paradigms @entity72163 and @entity72165 , but not @entity72164 , can modulate the pharyngeal microcircuit. Behavioural analysis identified @entity72163 as a significant determinant of the @entity3264 -dependent modulation of feeding, further highlighting the significance of mGluRs in complex @entity2199 behaviour.
