[A two-step protocol for induction of XXXX adipose tissue-derived stem cells into neuron-like cells].

@entity8160 @entity2136 @entity664 @entity1008 @entity10157 @entity2141 @entity1968 @entity35 @entity19744 @entity18715 @entity19465
OBJECTIVE: To investigate the differentiation potential of @entity35 adipose tissue-derived cells (ADSCs) into neuron-like cells in vitro using a two-step induction protocol. METHODS: ADSCs isolated from the epididymal fat pads in male SD @entity35 by means of differential attachment were cultured in vitro and subjected to adipogenic induction. After flow cytometric identification of the cell surface antigens CD106, @entity19465 , @entity1008 , CD49d, @entity18715 and CD29, the third-passage ADSCs were induced to transdifferentiate into neural stem cell (NSC)-like cells in DMEM/F12 medium containing 10 ng/ml @entity664 ( @entity664 ), 20 ng/ml @entity8160 . The resultant NSC-like cells were then induced to differentiate into neuron-like cells in the neurobasal medium containing 10 ng/ml @entity2136 ( @entity2136 ), 10 ng/ml @entity10157 ( @entity10157 ) and 1   mol/L @entity1968 (RA). Immunocytochemistry was employed to identify the expression of the cell surface markers nestin, @entity19744 and @entity2141 . RESULTS: The isolated ADSCs were positive for @entity18715 and CD29, and oil red O staining of the induced adipose-like cells yielded positive results. The third-passage ADSCs induced for 7 days aggregated as floating cell spheres positive for NSC surface antigen nestin. Further induction in neurobasal medium for 4 h resulted in adhesion of the cell spheres and the formation of cell processes extending from some peripheral cells, suggesting a morphological resemblance to neurons. Most of the cells showed positivity for @entity19744 and @entity2141 . CONCLUSION: ADSCs can be induced to differentiate into neuron-like cells in vitro under appropriate conditions.
