Rapid quantification of miglustat in XXXX plasma and cerebrospinal fluid by liquid chromatography coupled with tandem mass spectrometry.

@entity1 @entity35855 @entity1042 @entity1051 @entity6190 @entity1626 @entity8706 @entity2841 @entity32174
@entity32174 ( @entity32174 ) is an iminosugar recently introduced in therapeutic as potential alternative therapy in disorders found in several diseases such as @entity1051 , Gaucher or @entity8706 . A highly sensitive liquid-chromatography-electrospray tandem mass spectrometry (LC-MS/MS) assay was developed for the quantification of miglustat in @entity1 plasma and cerebrospinal fluid ( @entity2841 ). The sample preparation consists in a simple protein precipitation with a mixture of @entity1626 / @entity1042 (75/25) which yields 100% recovery. The isocratic separation utilizes an Atlantis Hilic (3 microm, 150 mm x 2.1 mm) column, with a mobile phase of @entity1626 /water/ @entity6190 buffer (75/10/15, v/v/v) delivered at 230 microl/min. Selected reaction monitoring (SRM) mode was used with the transitions m/z 220-->158 for the miglustat and m/z 208-->m/z 146 for the @entity35855 (internal standard). Good linearity was observed in a range from 125 to 2500 ng/ml and from 50 to 1000 ng/ml, for @entity2841 , respectively. The within-run precision of the assay was less than 6%, and the between-run run precision was less than 6.5%, for six replicates at each of three concentrations and evaluated on three separated days for both @entity2841 mediums. Assay accuracy was in the range of 98-106.5%. Stability of miglustat was reported under a variety of storage conditions. The miglustat concentrations in two @entity1 are presented to demonstrate the clinical interest of this new method.
