Experimental model of @entity9660 designed for rotational thromboelastometry in XXXX with congenital heart disease.

@entity1 @entity928 @entity9660 @entity927 @entity3479
UNASSIGNED: We assessed an in-vitro model of @entity9660 using rotational thromboelastometry (ROTEM) by the addition of increasing concentrations of @entity3479 ( @entity3479 ) on whole blood obtained from @entity1 undergoing cardiac surgery. We assessed the relevance of this model by repeating the tests in the same population after @entity927 ( @entity928 ) infusion. In addition, we determined the sensitivity and specificity of ROTEM parameters to detect the different degrees of fibrinolysis. Blood samples obtained from 20 @entity1 were analyzed at two predefined timepoints: after induction of anesthesia, before @entity928 (baseline), and at the end of surgery during @entity928 infusion (end surgery). At baseline, an extrinsic activation with tissue factor (EXTEM) test was performed without and with increasing concentration of @entity3479 (102, 255, 512, 1024, 1535, and 2539unitst-PA/ml). At the end of surgery, a second EXTEM test was performed without and with two different @entity3479 concentrations (1535 and 2539unitst-PA/ml). At baseline, increasing @entity3479 concentrations in the EXTEM test induced a gradual increase of @entity9660 characterized by a reduction in clot firmness and stability parameters. In the presence of @entity928 , @entity3479 -induced @entity9660 was completely abolished. Lysis-onset time (LOT) and degree of fibrinolysis measured at 30min (LI30) best assessed the degree of fibrinolysis. This in-vitro model of @entity3479 -induced @entity9660 using the EXTEM test of ROTEM may represent a promising tool to assess @entity9660 in the pediatric population. In addition, we observed that LOT and LI30 should be considered as the best parameters to detect different degrees of fibrinolysis.
