Activation of XXXX -   induces microRNA-100 and decreases the uptake of very low-density lipoprotein in endothelial cells.

@entity26719 @entity29852 @entity5601 @entity209 @entity28427 @entity65
BACKGROUND AND PURPOSE: Increased level of very low-density lipoprotein (VLDL) is a key feature of the @entity209 and is associated with @entity65 . @entity5601 -   agonists play a protective role in lipid metabolism and vascular function. In this study, we aimed to investigate the role of @entity5601 -   in the uptake of VLDL in endothelial cells and its underlying mechanism(s). EXPERIMENTAL APPROACH: Uptake of VLDL in HUVECs was assessed by Dil-fluorescent labelling of VLDL. Levels of @entity28427 mRNA and @entity26719 ) were detected by quantitative PCR. The target genes of @entity26719 were predicted using bioinformatics analysis. 3'-Untranslated region (3'-UTR) luciferase reporter and Argonaute 1 pull-down assays were used to validate the target of @entity26719 . KEY RESULTS: @entity5601 -   agonist @entity29852 decreased uptake of VLDL and expression of @entity28427 at mRNA and protein levels. @entity29852 inhibited the luciferase reporter activity of the 3'-UTR of @entity28427 . @entity28427 was a direct target of @entity26719 . @entity26719 was significantly increased by @entity29852 in HUVECs. Transfection of a @entity26719 mimic decreased the mRNA and protein levels of @entity28427 and uptake of VLDL. Furthermore, a @entity26719 inhibitor abolished the inhibitory effect of @entity5601 -   on @entity28427 expression and VLDL uptake. CONCLUSIONS AND IMPLICATIONS: In endothelial cells, activation of @entity5601 -   decreased @entity28427 expression and VLDL uptake via the induction of @entity26719 . These results provided a novel mechanism for the vascular protective effect of @entity5601 -   agonists.
