Modified fields' stain: ideal to differentiate Dientamoeba fragilis and XXXX sp.

@entity1 @entity2194 @entity17056 @entity344 @entity6599 @entity325 @entity8184
UNASSIGNED: Dientamoeba fragilis, a trichomonad parasite is usually found in the gastrointestinal tract of @entity1 , and it is known to be the cause for @entity344 . The parasite is globally distributed and mostly found in rural and urban areas. The parasite is found in @entity1 and nonhuman primates such as the macaques, @entity6599 , and @entity17056 . Often, the parasite is confused with another largely found organism in stools called @entity8184 sp. especially when seen directly under light microscopy on culture samples containing both parasites. Both sometimes are seen with two nuclei with sizes tending to be similar which complicates identification. Stools were collected fresh from nine previously diagnosed @entity1 infected with D. fragilis who also were found to be positive for @entity8184 sp. Samples were then cultured in Loeffler's medium and were stained with Giemsa, iron hematoxylin, and modified Fields' ( @entity2194 ) stain, respectively. D. fragilis was differentiated from @entity8184 sp. when stained with @entity2194 stain by the presence of a thinner outer membrane with clearly demarcated nuclei in the center of the cell whilst @entity8184 sp. had a darker and thicker stained outer membrane with the presence of two nuclei. The staining contrast was more evident with modified Fields' stain when compared with the other two. The simplicity in preparing the stain as well as the speed of the staining procedure make @entity2194 stain an ideal alternate. The modified Fields' stain is faster and easier to prepare when compared to the other two stains. @entity2194 stain provides a better contrast differentiating the two organisms and therefore provides a more reliable diagnostic method to precisely identify one from the other especially when cultures show @entity325 .
