Sequential changes in autophagy in XXXX .

@entity82 @entity6 @entity3839 @entity28190 @entity299 @entity35
Autophagy is considered to be associated with @entity299 . However, whether autophagy accelerates or ameliorates @entity82 remains to be elucidated. In the present study, 36 @entity35 were divided into two groups: Control @entity35 and @entity6 @entity35 . The @entity6 @entity35 were established by feeding the animals a high fat diet combined with @entity3839 . From the two groups, six @entity35 were sacrificed after 1, 6 and 7 months. Cardiac systolic functions were measured. The collagen volume fraction was calculated using Masson's trichome staining and the mRNA expression levels of type  -I and type  -III collagen were measured using reverse transcription  -quantitative polymerase chain reaction (RT  -qPCR) to assess the levels of @entity299 . The protein contents of microtubule  -associated protein 1 light chain 3 (LC3) and sequestosome 1 ( @entity28190 ) were evaluated using western blotting, and the mRNA expression of Beclin 1 was measured using RT  -qPCR, in order to assess autophagy. The results revealed that, in the @entity6 @entity35 , @entity299 developed and cardiac systolic function was reduced. In the hearts of the @entity6 @entity35 , the mRNA expression levels of collagen type I and III, and Beclin1 were upregulated; the ratio of the protein level of LC3  -II/LC3  -I was increased and the content of @entity28190 was decreased. All the changes were aggravated as time increased. The changes in autophagy were correlated with those of @entity299 , suggesting that autophagy may have a synergistic role in @entity299 .
