Assessment of serum biomarkers in XXXX after exposure to pesticides of different chemical classes.

@entity5879 @entity276 @entity844 @entity17284 @entity535 @entity20346 @entity315 @entity35 @entity316 @entity2359 @entity5051
UNASSIGNED: There is increasing emphasis on the use of biomarkers of adverse outcomes in safety assessment and translational research. We evaluated serum biomarkers and targeted metabolite profiles after exposure to pesticides ( @entity315 , @entity316 , @entity5051 , @entity17284 , @entity20346 , @entity276 ) with different @entity2359 . Adult male Long-Evans @entity35 were evaluated after single exposure to vehicle or one of two doses of each pesticide at the time of peak effect. The doses were selected to produce similar magnitude of behavioral effects across chemicals. Serum or plasma was analyzed using commercial cytokine/protein panels and targeted metabolomics. Additional studies of @entity276 used lower doses (lacking behavioral effects), singly or for 14days, and included additional markers of exposure and biological activity. Biomarker profiles varied in the number of altered analytes and patterns of change across pesticide classes, and discriminant analysis could separate treatment groups from control. Low doses of @entity276 produced greater effects when given for 14days compared to a single dose. Changes in thyroid hormones and relative amounts of @entity276 and its @entity5879 metabolite also differed between the dosing regimens. Most cytokine changes reflected alterations in inflammatory responses, hormone levels, and products of phospholipid, @entity844 , and @entity535 metabolism. These findings demonstrate distinct blood-based analyte profiles across pesticide classes, dose levels, and exposure duration. These results show promise for detailed analyses of these biomarkers and their linkages to biological pathways.
