[Effect of Jiawei Foshou San and its compatibility on hepatic P450 enzyme activity and hepatocyte morphology in XXXX ].

@entity137 @entity28233 @entity19316 @entity1711 @entity22934 @entity1710 @entity11920 @entity35 @entity7771 @entity23943
To investigate the effect of Jiawei Foshou San and its various combined administration on hepatic P450 enzyme activity and hepatocyte morphology in @entity35 . @entity35 were orally administered with drugs for four weeks and then sacrificed to prepare liver microsomes. The liver microsomes were incubated with the cocktail method; The metabolites were determined with the rapid liquid chromatography with tandem mass spectrometry (LC-MS/MS) to investigate the hepatocyte P450 enzyme activity. In addition, the hepatic pathological changes were observed by using the @entity1710 and @entity1711 (HE) staining. Compared with the control group, the enzyme activity of @entity7771 and CYP3A4 in the Jiawei Foshou san group showed a significant rise (P < 0.05); the enzyme activity of @entity7771 , CYP2C9, @entity22934 , @entity11920 and CYP3A4 in the @entity28233 + @entity19316 group and the @entity19316 + @entity23943 group showed a significant rise (P < 0.05) ; the enzyme activity of @entity7771 , @entity22934 and @entity11920 in the @entity19316 group showed a significant rise (P < 0.05); the enzyme activity of CYP3A4 in the @entity28233 group showed a significant reduction (P < 0.05). After the administration with various drugs, the hepatocyte morphologies in the @entity28233 group and the @entity19316 group were normal. The pathological changes were observed in the @entity23943 group, such as unclear boundary of hepatic lobules, disordered hepatic cell arrangement, blurred edge, anisokaryosis and infiltration of inflammatory cells. The @entity28233 + @entity23943 group, the @entity19316 + @entity23943 group and the Jiawei Foshou San group also showed inflammatory infiltration, but with less pathological changes, particularly the Jiawei Foshou San group. The study result shows that Jiawei Foshou San can induce the enzyme activity of @entity7771 and CYP3A4, and @entity19316 may be the effective substance for inducing @entity7771 . Its combination with @entity28233 and @entity19316 can significantly reduce the liver @entity137 of @entity23943 .
