Proteomic Profiling of XXXX Small Cell Lung Cancer Cell Line NCI-H211.

@entity1 @entity575 @entity5 @entity215 @entity151 @entity204
PURPOSE: @entity151 is one of the major causes of @entity204 from @entity5 worldwide. To explore the expressions of global protein in @entity151 cells, a proteomic approach, to identify the proteins, was used and the establishment of a protein reference map attempted. MATERIALS AND METHODS: Two-dimensional gel electrophoresis (2-DE), with subsequent analysis by mass spectrometry (MS), was applied to the study of protein identification from a @entity151 cell line, @entity1 . The cells were lysed, and the extracts subjected to isoelectric focusing, with immobilized pH gradients, followed by second dimension SDS-PAGE. The polypeptides were identified by peptide mass fingerprinting, with @entity215 , after in-gel protein digestion. RESULTS: From @entity575 staining of the gel, around two thousands protein spots were separated by the 2-DE. Of these protein spots visualized in the gel, one hundred and ten were identified by peptide mass fingerprinting. Different proteins, such as enzymes, cytoskeletal proteins and proteins common to eukaryotic cells, were identified. CONCLUSION: The protein expressions of the @entity151 cells were analyzed to establish a protein reference map. The reference map presented here may serve as a working tool for the further study of @entity151 .
