Repetitive XXXX in @entity1707 is associated with the increased expression of inducible and constitutive @entity842 synthases.

@entity1707 @entity33368 @entity842 @entity1875 @entity1562 @entity432
OBJECTIVES: @entity842 (NO) has complex effects on myocardial function particularly following ischaemia-reperfusion. The goal of this study was to examine the result of repetitive @entity1562 on myocardial NO release and expression of inducible ( @entity33368 ) and constitutive (eNOS) NO synthases. METHODS AND RESULTS: @entity1875 anaesthetised @entity1707 underwent ten, 2-min episodes of circumflex @entity432 (n = 6) or acted as sham operated controls (n = 4). Measurements of segment shortening demonstrated a fall in function in the ischaemic territory to 52.5 +/- 7.3% (mean +/- S.E.M.) of baseline shortening 30 min after the stunning stimulus, recovering to 92 +/- 8.7% 5.5 h later. Function remained stable in sham controls. The change in venous-arterial [NO] between baseline and 6 h reperfusion was found to be significantly different between the two groups (0.2 +/- 0.7 in stunned vs. -4.3 +/- 1.6 microM in shams; P < 0.02). Western blotting and band optical density used to compare tissue from stunned territory (S), non-stunned territory (IC) and sham control animals (SC) demonstrated this was associated with an increase in the expression of both @entity33368 (S: 93 +/- 13.4, IC: 37 +/- 2.4 and SC: 25 +/- 4 [arbitrary units], P < 0.01 and P = 0.031) and eNOS (S: 104 +/- 7.4, IC; 62.5 +/- 7.4 and SC; 75.7 +/- 0.6, P < 0.03 and P < 0.01) in stunned @entity1562 . Immunocytochemistry localised @entity33368 reactivity to vascular smooth muscle cells and cardiomyocytes in stunned tissue and eNOS reactivity to endothelial cells. CONCLUSION: Recovery from repetitive @entity1562 is associated with the increased expression of both @entity33368 and eNOS and would be compatible with a protective role for both these enzymes. This finding has possible relevance for both the late window of ischaemic preconditioning and @entity1562 .
