Time-related XXXX in stored shed mediastinal blood after cardiopulmonary bypass.

@entity2142 @entity1 @entity2100 @entity20844
Reinfusion of mediastinal shed blood during cardiopulmonary bypass reportedly reduces the need for homologous blood transfusion. Although the @entity2100 is thought to be amplified by shear stress during cardiopulmonary bypass and processing, the time-related deterioration of red blood cells (RBCs) in stored shed blood has not been studied extensively. In this study, we examined time-related @entity2142 in shed blood stored at different temperatures. We examined processed shed blood collected from 15 @entity1 (11 @entity1 and 4 @entity1 ; mean age      standard deviation, 71      9  years) during cardiopulmonary bypass. The shed blood was collected and stored at 20  C (group A) or 4  @entity20844 ). Stored blood collected by venipuncture at the end of the surgery was used as a control. Damage was assessed by measuring its free hemoglobin (Hb) levels, using a photometric assay. The free Hb levels in blood samples from each group were tested at 0, 3, 6, 12, 24, 36, and 48  h after surgery. The free Hb levels (g/dl) at 0, 12, and 24  h were 0.03      0.01, 0.05      0.02*, and 0.06      0.02* in group A; 0.03      0.02, 0.04      0.03, and 0.05      0.02* in group B; and 0.01      0.01, 0.01      0.01, and 0.01      0.01 in the control group (*p  <  0.05 vs. 0  h after surgery). The free Hb levels in stored shed blood significantly increased after 12  h in group A (20  C) and increased after 24  h in group B (4  C), whereas in drawn blood, they did not significantly increase over the first 24  h. Compared to storage at 20  C, storage at 4  C suppresses the increase in the free Hb levels.
