Reconstitution of XXXX repertoire following HLA-matched hematopoietic cell transplantation.

@entity1 @entity9371 @entity9707 @entity5 @entity281 @entity1778 @entity9706 @entity4107
Interactions between killer immunoglobulin-like receptors (KIRs) and human @entity4107 ligands influence development of natural killer (NK) cell repertoire and response to @entity281 , @entity5 , and allogeneic tissue. As KIRs and HLA class I molecules are highly polymorphic, clinical allogeneic hematopoietic cell transplantation is predicted to frequently involve @entity9371 mismatch, and thus to provide a unique system for study of @entity1 @entity9706 repertoire development. Eighteen @entity1778 @entity1 undergoing HLA-matched transplantation and their donors were analyzed for @entity9371 genotype. Ten of 13 HLA-identical donor- @entity1 pairs were @entity9371 mismatched and 3 were matched; all HLA-matched unrelated pairs were @entity9371 mismatched. Reconstitution of recipient NK cell repertoire following transplantation was examined using flow cytometry and monoclonal antibodies specific for @entity9371 and @entity9706 : @entity9707 . These data form 3 groups. Six to 9 months after transplantation, 8 @entity1 (group 1) reconstituted an NK cell repertoire resembling that of their donor, and for @entity9371 -mismatched transplants, distinct from the recipient before transplantation. In the first year after transplantation, 5 @entity1 (group 2) exhibited a generally depressed frequency of @entity9371 -expressing NK cells and concomitant high frequency of @entity9706 : @entity9707 expression. By 3 years after transplantation, the frequency of @entity9371 -expressing NK cells had increased to donor values, in the 3 @entity1 from group 2 analyzed for this period. The remaining 5 @entity1 experienced severe clinical complications following transplantation and displayed unique features in their @entity9706 reconstitution. These results demonstrate that a majority of HLA-matched hematopoietic cell transplantations involve @entity9371 mismatch and reveal differences in NK cell repertoire having potential impact for immune responsiveness and transplantation outcome.
