A multivariate analysis using physiology and behavior to characterize robustness in two isogenic lines of XXXX exposed to a confinement stress.

@entity5660 @entity26 @entity701 @entity146
UNASSIGNED: Robustness is a complex trait difficult to characterize and phenotype. In the present study, two features of robustness in @entity5660 were investigated: sensitivity and resilience to an acute stressor. For that purpose, @entity26 consumption, @entity701 release, group dispersion and group activity of two isogenic lines of juvenile @entity5660 were followed before and after an environmental challenge. The effect of a 4h confinement protocol (~140kg/m(3)), which is generally considered as a highly stressful challenge, was investigated. Temporal patterns produced by this experiment were analyzed using multivariate statistics on curve characteristics to describe physiological and behavioral adaptive systems for each isogenic line. The two isogenic lines were found to be highly divergent in their corticosteroid reactivity. However, no correlation between physiological and behavioral sensitivity or resilience was observed. Furthermore, the multivariate analysis results indicated two separate and independent fish group coping strategies, i.e. by favoring either behavioral or physiological responses. In addition, considerable intra-line variabilities were observed, suggesting the importance of micro-environment effects on perturbation sensitivities. In this context, @entity701 release rate variability was found to be related to the pre-stress social environment, with a strong correlation between pre-stress @entity146 and @entity701 release rate amplitude. Overall, this approach allowed us to extract important characteristics from dynamic data in physiology and behavior to describe components of robustness in two isogenic lines of @entity5660 .
