Interaction of XXXX promoters with epithelial cells in culture. An immunofluorescence study.

@entity235 @entity5 @entity72289 @entity2823 @entity2389 @entity865 @entity4514
@entity4514 ( @entity865 ) has a profound and rapid influence on the cytoskeleton of Madin-Darby @entity235 Kidney (MDCK) cells. Within 10 min, @entity865 induces a rapid change in morphology, from a flat, cuboidal state to a rounded or elongated morphology in which the cell membranes become convoluted. Concomitant with this morphological change is a rapid dissolution of stress fibres and a redistribution of F-actin from microfilament bundles to a membrane or sub-membranous location. The rearrangement of actin is paralleled by a rearrangement of alpha-actinin and a reduction in the number of @entity72289 -containing adhesion plaques. Unusual F-actin configurations are often found emanating from a perinuclear location, usually containing alpha-actinin and terminating in a @entity72289 -containing adhesion plaque. The cytoskeletal rearrangements occur in the presence of inhibitors of protein synthesis or oxidative phosphorylation, but do not occur if glycolysis is also inhibited. The rearrangements are partly abrogated by the presence of @entity2389 (CB). Despite these dramatic changes in microfilaments the polymerization state of actin remained unaltered after @entity865 treatment. Furthermore, although changes in the movement of membrane lipids have been reported, no obvious differences in the ability of glycoproteins to redistribute in the plane of the membrane were found as judged by @entity2823 -concanavalin A (conA) induced patching. The rapidity of the morphological response of MDCK cells to @entity865 indicates that the cytoskeleton is one of the primary targets of @entity865 , but that @entity5 promoters differ from RNA @entity5 viruses in their effect on the state of actin polymerization.
