Expression of purinergic P2X7 receptor in XXXX brain during the symptomatic phase of experimental autoimmune encephalomyelitis and after recovery of neurological deficits.

@entity35 @entity16 @entity22324 @entity6503 @entity15
Purinergic ionotropic P2X(7) receptor is widely distributed in brain. Strong evidence suggests that this receptor is related to inflammatory and neurodegenerative changes in many pathological states of central nervous system (CNS), including @entity15 ( @entity15 ). @entity6503 ( @entity6503 ) is the commonly used animal model of @entity15 . In this study we investigate the expression of P2X(7)R protein in @entity35 brain in the symptomatic phase of @entity6503 (day 10 post immunization) and after reversion of @entity16 (day 20 p.i.). We found the increased level of P2X(7)R protein in brain homogenates of @entity6503 @entity35 in both examined time windows. Immunohistochemical study revealed enhanced receptor's immunoreactivity. Immunoblots done with isolated cellular brain fractions indicated that the P2X(7)R overexpression is related to synaptosomal fraction in the symptomatic phase and to the glial ( @entity22324 ) fraction in the recovery phase of @entity6503 . Concomitantly, we noticed overexpression of astroglial marker GFAP in brain homogenates and astroglial fraction ( @entity22324 ), so as its enhanced immunoreactivity in brain sections (10 days p.i.) which did not decline to control values in the recovery phase, similarly to P2X(7)R expression. Results suggest the involvement of P2X(7)R-mediated signaling in the pathomechanisms of @entity6503 with the possible relevance of astrocytic pool of cells.
