Renal in situ hybridization studies of extracellular matrix related molecules in XXXX .

@entity1 @entity217 @entity8 @entity6 @entity1476 @entity2316 @entity1859 @entity1549 @entity2843
BACKGROUND/AIM: Progressive expansion of mesangial matrix and @entity8 membrane thickening represent alterations in the balance between synthesis and degradation of glomerular extracellular matrix (ECM) protein and are hallmarks of @entity2316 . In order to elucidate the basis for this imbalance between the synthesis and the degradation of ECM in renal tissues from @entity1 of @entity6 ( @entity6 ) with @entity2316 ( @entity2316 ), we examined the expression of alpha1 chain of type IV collagen (IV-C), matrix metalloproteinase-2 and -3 ( @entity1476 , @entity1549 ), @entity2843 ( @entity2843 ) and @entity217 mRNA using a high-resolution in situ hybridization with @entity1859 -labeled oligonucleotide. METHODS: @entity1 were divided into two groups based on both of degree of mesangial expansion using electron microscopic point counting morphometric methods and duration of @entity6 : 7 'fast-track' @entity1 were selected for their very rapid development of @entity2316 structural changes and 8 'slow-track' @entity1 for their very slow development of @entity2316 structural changes. Seven normal @entity1 kidney (NHK) tissues were used as controls. RESULTS: Positive cells for each mRNA were observed in glomerular resident cells, including glomerular mesangial, epithelial and endothelial cells and cells of Bowman's capsule. The percentage of glomerular cells positive for IV-C, @entity1476 and @entity1549 mRNA was significantly greater in the 'slow-track' vs. 'fast-track' @entity1 . No significant differences in percentage positive cells was seen for @entity217 mRNA. Furthermore, to elucidate the total number of positive cells per glomerulus for each mRNA, we estimated total cell number of glomerulus using morphometric techniques on light microscopy tissues. The total cell number per glomerulus was significantly greater in 'fast-track' than that in 'slow-track' @entity1 and NHK. The total number of positive cells per glomerulus for @entity1476 in NHK was significantly greater than that in 'slow-track' and 'fast-track' @entity1 . CONCLUSIONS: Thus, IV-C, @entity1476 , @entity1549 and @entity2843 mRNA are expressed in resident glomerular cells in renal tissues from NHK and @entity6 . Glomerular alterations in these in situ mRNA expressions sufficient to explain ECM accumulation and @entity2316 risk were not uncovered. These largely negative results could be due to methodologic quantitative imprecision or could indicate that post-translational differences account for ECM imbalance in @entity2316 . However, these studies make it clear that unraveling the nature of the ECM production/removal imbalance in @entity2316 will require careful consideration of alterations in glomerular cell number.
