XXXX growth using magnetic resonance and bioluminescence imaging.

@entity1 @entity788 @entity19 @entity5 @entity4897
OBJECT: @entity788 is one of the most lethal @entity1 @entity5 and appropriate experimental @entity5 models are needed for the development of innovative therapeutic approaches. This paper describes an experimental model of @entity1 @entity788 and a related non invasive imaging technique suitable for monitoring @entity5 growth and metastatization. The aim of the work was the implementation of an experimental platform suitable for assessing the efficacy of new therapeutic agents. MATERIALS AND METHODS: @entity1 @entity788 cells (PANC-1-Luc+) were injected into the pancreas of female athymic @entity4897 @entity19 . Magnetic Resonance Imaging (MRI) at 4.7T and Bioluminescence Imaging (BLI) were performed in each @entity19 at three time points after cell inoculation (1, 2 and 3months). Two groups of @entity19 were studied: the first group of n=13 @entity19 in which 5*10(6) cells were injected and the second group of n=10 @entity19 in which 2*10(6) cells were injected. MRI examination included T2w acquisitions and (at the last time point) Dynamic-contrast-enhanced-MRI (DCE-MRI). RESULTS: Each @entity19 underwent three longitudinal MRI and BLI examinations. BLI was more sensitive than MRI producing higher detection rate at early time points. Moreover in one case of abdominal dissemination of @entity788 cells, small tumoral masses were detected by BLI and not detected by MRI. However BLI appears more prone to experimental error most likely due to photon attenuation. In 4 @entity19 BLI produced false negative results. DCE-MRI experiments providing information on @entity5 perfusion were conducted successfully in this anatomical district and demonstrated that the @entity5 tissues from the second experimental group are more vascularized compared to the first group. CONCLUSION: The present study performed on the experimental model of @entity788 here described shows that MRI and BLI are complementary techniques and that synergistic application of both can overcome the intrinsic limitations of each.
