Type 2 Diabetes Monocyte MicroRNA and mRNA Expression: XXXX Associates with Increased Differentiation-Related Genes but Not Inflammatory Activation.

@entity2175 @entity32 @entity1 @entity6460 @entity38963 @entity23022 @entity2299 @entity4639 @entity21478 @entity23638 @entity3840
OBJECTIVE: To study the expression pattern of microRNAs and mRNAs related to @entity32 in @entity2175 monocytes. DESIGN: A microRNA finding study on monocytes of @entity2175 @entity1 and controls using array profiling was followed by a quantitative Real Time PCR (qPCR) study on monocytes of an Ecuadorian validation cohort testing the top over/under-expressed microRNAs. In addition, monocytes of the validation cohort were tested for 24 @entity32 -related mRNAs and 2 microRNAs previously found deregulated in (auto)-inflammatory monocytes. RESULTS: In the finding study, 142 significantly differentially expressed microRNAs were identified, 15 having the strongest power to discriminate @entity2175 @entity1 from controls (sensitivity 66%, specificity 90%). However, differences in expression of these microRNAs between @entity1 and controls were small. On the basis of >1.4 or <0.6-fold change expression 5 microRNAs were selected for further validation. One microRNA (miR-34c-5p) was validated as significantly over-expressed in @entity2175 monocytes. In addition, we found over expression of 3 mRNAs ( @entity6460 , @entity21478 and @entity38963 ) in the validation cohort. These mRNAs are important for cell morphology, adhesion, shape change, and cell differentiation. Classical inflammatory genes (e.g. @entity4639 ) were only over-expressed in monocytes of @entity1 with normal serum lipids. Remarkably, in @entity2299 , there was a reduction in the expression of inflammatory genes (e.g. @entity23638 , @entity23022 and @entity3840 ). CONCLUSIONS: The expression profile of microRNAs/mRNAs in monocytes of @entity2175 @entity1 indicates an altered adhesion, differentiation, and shape change potential. Monocyte inflammatory activation was only found in @entity1 with normal serum lipids. Abnormal lipid values coincided with a reduced monocyte inflammatory state.
