[Effect of electroacupuncture of "Guanyuan" (CV 4) on Wnt-b-catenin signaling in XXXX @entity35 ].

@entity6037 @entity6035 @entity170 @entity9356 @entity35
OBJECTIVE: To observe the effect of electroacupuncture of "Guanyuan" (CV 4) on bone miner density, bone biomechanics, serum @entity9356 ( @entity9356 ) and alkaline phosphatase (ALP) contents, and femoral osteoblastic Wnt-b-catenin signaling in @entity170 ( @entity170 ) @entity35 . METHODS: Forty female SD @entity35 were randomly divided into sham operation (sham), model, EA-CV 4, and EA-non-acupoint (below the costal region) groups (n = 10 in each group). The @entity170 model was established by performing an ovariectomy in the @entity35 of the later 3 groups. EA (2 Hz, 1 mA) was applied to CV 4 for 20 min, once daily for one month, with one day's break between every 10 days. After the treatment, serum @entity9356 and ALP contents were detected using ELISA, the right femoral bone miner density and biomechanics (maximum load, breakage load) were measured using a Dual Energy X-Ray Bone Densitometer and a Universal Material Testing Instrument, respectively. The expression levels of @entity6035 mRNA, b-catenin mRNA and the bone-specific factors runt-related transcription factor 2 ( @entity6037 ) mRNA of the femoral bone tissue were determined by real time RT-PCR. RESULTS: HE staining results suggested EA of "Guanyuan" (CV 4) can improve the morphological changes (trabeculae) of @entity170 in ovariectomized @entity35 . Compared with the control group, femoral maximum load and breakage load, bone density and serum @entity9356 and ALP contents, femoral @entity6035 mRNA, b-catenin and @entity6037 mRNA expression levels, and femoral @entity6035 .and b-catenin immunoactivity were significantly down-regulated in the ovariectomized @entity35 (model group) (P < 0.05). Following EA treatment, all the decreased levels of femoral maximum load and breakage load, bone density and serum @entity9356 and ALP, femoral @entity6035 mRNA and protein, b-catenin mRNA and protein and @entity6037 mRNA expression were obviously reversed in the EA-CV 4 group (P < 0.05) rather than in the non-acupoint group (P > 0.05). CONCLUSION: EA of CV 4 can improve the femoral biomechanics, increase bone density in OVX @entity35 , which are associated to its effects in up-regulating levels of serum @entity9356 and ALP, femoral @entity6035 mRNA and protein, b-catenin mRNA and protein and @entity6037 mRNA expression, suggesting an involment of improved bone metabolism and activation of osteoblastic Wnt-b-catenin signaling.
