Histopathologic and ultrastructural studies on experimental caprine XXXX .

@entity7843 @entity149 @entity1237 @entity528
The distribution pattern and associated tissue reactions with progressive changes in Besnoitia caprae cysts were investigated in 6 experimentally infected 16- to 20-month-old male @entity1237 . Each @entity1237 was subcutaneously inoculated with approximately 13   10(8) B caprae bradyzoites. The animals were examined daily for development of clinical @entity7843 , and skin biopsies from distal parts of the limbs were taken at weekly intervals. At 15, 30, 60, 120, 180, and 365 days postinfection (DPI), 1 @entity1237 was euthanized. Samples were collected at autopsy from various organs for histologic and ultrastructural studies. No cysts were seen in tissue sections on 15, 30, and 365 DPI, but large numbers were present at 60, 120, and 180 DPI in the skin of the distal limbs, scrotum, and ears, with fewer in the tongue, palate, sclera, testicles, and spermatic cord. No cysts were seen in the lungs, liver, kidneys, @entity149 , or lymph nodes. Cyst numbers peaked at 60 DPI, then declined from 120 to 180 DPI. @entity528 were relatively rare at 60 DPI but more numerous at 180 compared with 120. A granulomatous reaction--predominantly characterized by macrophages, lymphocytes, and plasma cells--surrounded each degenerated cyst. All @entity1237 showed testicular tubular degeneration with little or no spermatogenic activity. The sizes of cysts and their wall thickness, with the size of bradyzoites and some of their organelles, exhibited progressive chronologic changes.
