Labile and stable glycosylated hemoglobin during OGTT in healthy subjects and maturity-onset XXXX .

@entity4051 @entity413 @entity6
@entity4051 levels were determined by a rapid chromatographic column test in 15 healthy subjects (HS) and in 15 maturity-onset @entity6 (MOD), fasting and 2 h after @entity413 ingestion (100 g for HS, 50 g for MOD). Chromatography was carried out both before and after 6 h of red cell incubation in saline at 37 degrees C. @entity4051 in HS at 0 and 120 min of OGTT was not significantly different, either before (6.24 +/- 0.61% and 6.22 +/- 0.62%) or after red cell incubation in saline (5.85 +/- 0.61% and 5.87 +/- 0.55%). Red cell incubation in saline significantly reduced @entity4051 levels both at 0 and 120 min (2p less than 0.001). @entity4051 in MOD before red cell incubation in saline, was slightly but significantly higher at 120 min (8.61 +/- 1.03%) than at 0 min (8.39 +/- 1.01%): 2p less than 0.001. After incubation in saline, this difference was cancelled (7.86 +/- 0.85% at 0 min and 7.97 +/- 0.83% at 120 min: n.s.). Post-incubation levels were lower than pre-incubation ones both at 0 and 120 min (2p less than 0.001). The @entity4051 increment observed in MOD is significantly correlated (p less than 0.01; r=0.64) to the blood @entity413 increment observed at the glycemic peak. We conclude that hemoglobin glycosylation may show rapid changes also in MOD, reflecting blood @entity413 changes, whereas in HS the physiological glycemic excursions are not wide enough to produce rapid @entity4051 changes. Since labile and stable @entity4051 co-elute in the rapid chromatographic methods, red cell incubation in saline for 6 h at 37 degrees C is recommended as a simple procedure which allows the measurement of the stable fraction alone, i.e. the real index of long-term glycemic control, independent of rapid glycemic fluctuations.
