Correlation between @entity308 of catabolite control of @entity6122 metabolism and a defect in the @entity7954 : XXXX phosphotransferase system in @entity36965 .

@entity6122 @entity7955 @entity308 @entity7954 @entity11064 @entity10254 @entity36965 @entity3913 @entity46790 @entity413 @entity897 @entity3776
@entity36965 X-160 which lacks catabolite control by @entity413 was isolated from nature (soy moromi mash). Wild-type strains, in @entity6122 - @entity413 medium, utilized @entity413 preferentially over @entity6122 and showed diauxic growth. With wild-type strain I-13, @entity6122 isomerase activity was not induced until @entity413 was consumed from the medium. Strain X-160, however, utilized @entity6122 concurrently with @entity413 and did not show diauxic growth. In this strain, @entity6122 isomerase was induced even in the presence of @entity413 . @entity413 transport activity in intact cells of strain X-160 was less than 10% of that assayed in strain I-13. Determinations of glycolytic enzymes did not show any difference responsible for the unique behavior of strain X-160, but the rate of @entity3776 formation with @entity7954 ( @entity7955 ) as a @entity11064 donor in permeabilized cells was less than 10% of that observed in the wild type. Starved @entity36965 I-13 cells contained the glycolytic intermediates @entity10254 , @entity46790 , and @entity7955 ( @entity7955 pool). These were consumed concomitantly with @entity413 or @entity897 uptake but were not consumed with @entity6122 uptake. The @entity413 transport system in @entity36965 was identified as a @entity7955 : @entity3913 phosphotransferase system on the basis of the substrate specificity of @entity7955 pool-starved cells. It is concluded that, in @entity36965 , this system is functional as a main @entity413 transport system and that defects in this system may be responsible for the @entity308 of @entity413 -mediated catabolite control.
