Expression profile of IGF paralog genes in liver and muscle of a GH-transgenic XXXX .

@entity2086 @entity71553 @entity22820 @entity1184 @entity71552 @entity22822 @entity61096 @entity37650 @entity71551 @entity3392 @entity22819
UNASSIGNED: The objective of this study was to investigate the relationship between IGFs produced in the liver and skeletal muscle with @entity2086 previously observed in a line of GH-transgenic @entity3392 . In this sense, we evaluated the expression of genes related to the IGF system in liver and muscle of transgenics, as well as the main intracellular signaling pathways used by GH/IGF axis. Our results showed an increase in expression of @entity22819 , @entity22820 , and @entity22822 genes in the liver. Moreover, there was a decrease in the expression of @entity37650 and an increase in muscle igf2r of transgenics, indicating a negative response of muscle tissue with respect to excess circulating IGFs. Muscle IGFs expression analyses revealed a significant increase only for @entity22822 , accompanied by a parallel induction of @entity71551 gene. The presence of @entity71551 may potentiate the @entity22820 action in muscle cells differentiation. Regarding JAK/ @entity71552 -related genes, we observed an alteration in the expression profile of both @entity61096 and @entity71553 in transgenic fish liver. No changes were observed in the muscle, suggesting that both tissues respond differently to GH-transgenesis. Western blotting analyses indicated an imbalance between the phosphorylation levels of the proliferative (MEK/ERK) and @entity1184 (PI3K/Akt) pathways, in favor of the latter. In summary, the results of this study suggest that the @entity1184 caused by GH-transgenesis in @entity3392 may be due to circulating IGFs produced by the liver, with an important participation of muscle @entity22822 . This group of IGFs appears to be favoring the @entity1184 intracellular pathway in muscle tissue of transgenic @entity3392 .
