The effect of urocortin 1 on motility in isolated, vascularly perfused XXXX colon.

@entity4033 @entity1169 @entity1022 @entity5521 @entity3897 @entity35 @entity694
BACKGROUND/AIMS: Urocortin 1, a corticotropin-releasing factor related peptide, @entity694 under stressful conditions. We investigated the effect of urocortin 1 on @entity694 using an experimental model with isolated @entity35 colon in which the blood flow and intestinal nerves were preserved. Furthermore, we assessed whether this effect was mediated by adrenergic or cholinergic nerves. METHODS: Colonic motility was measured in the proximal and distal parts of resected @entity35 colon. The colon resected from the peritoneum was stabilized, and then urocortin 1 (13.8, 138, 277, and 1,388 pM) was administered via a blood vessel. Motility index was measured in the last 5 min of the 15 min administration of urocortin 1 and expressed as percentage change from baseline. Subsequently, the change in motility was measured by perfusing urocortin 1 in colons pretreated with @entity5521 , @entity1169 , @entity4033 , @entity3897 , or @entity1022 . RESULTS: At concentrations of 13.8, 138, 277, and 1,388 pM, urocortin 1 increased the motility of proximal colon (20.4    7.2%,48.4    20.9%, 67.0    25.8%, and 64.2    20.9%, respectively) and the motility of distal colon (3.3    3.3%, 7.8    7.8%, 71.1    28.6%,and 87.4    32.5%, respectively). The motility induced by urocortin 1 was significantly decreased by @entity3897 to 2.4    2.4% in proximal colon and 3.4    3.4% in distal colon (p <   0.05). However, @entity1022 , @entity1169 , @entity5521 , and @entity4033 did not inhibit motility. CONCLUSIONS: Urocortin 1 increased @entity694 and it is considered that this effect was directly mediated by local muscarinic cholinergic receptors.
