Coordinated expression of the XXXX receptor and the @entity2290 receptor in metastatic @entity320 cells.

@entity1 @entity2290 @entity10431 @entity1093 @entity3 @entity4732 @entity320 @entity9615
@entity9615 is a marker of progression in @entity320 . Previously we reported that @entity1 @entity320 cells derived from regional lymph node @entity3 had increased alpha v @entity1093 -mediated adhesion to lymph node @entity4732 . In the present study, the expression and function of @entity1093 were further investigated with emphasis on the functional relationship between @entity1093 and the @entity2290 system of proteolysis. We found that @entity3 -derived @entity320 MeWo LNI 6I (6I) and MIM/8 LNI cells had a markedly increased expression of alpha v mRNA transcripts relative to the parent lines which was reflected in significantly elevated levels of the alpha v @entity1093 heterodimers on the cell surface. These cells also expressed elevated levels of @entity10431 ( @entity10431 ) mRNA and had higher levels of surface bound urokinase plasminogen activator as detected by immunolabeling. To determine whether the expression of @entity10431 and alpha v were linked, alpha v synthesis in the metastatic @entity320 cells was suppressed using alpha v antisense phosphorothioate oligonucleotides. This resulted in a marked decrease in detectable alpha v mRNA and protein and a corresponding substratum-specific reduction in cell adhesion to @entity4732 . When @entity10431 expression in these cells was subsequently analyzed, we found a reduction of approximately 50% in @entity10431 mRNA levels. On the other hand, ligation of the alpha v @entity1093 receptor on the @entity320 cells by immobilized antibody resulted in a twofold increase in @entity10431 mRNA. The results suggest that the expression of @entity10431 in metastatic @entity320 cells is linked to the expression and function of the @entity4732 receptor.
