Analysis of genetic alterations, classified according to their DNA ploidy pattern, in the progression of XXXX and early @entity14 .

@entity124 @entity2007 @entity894 @entity5 @entity189 @entity876 @entity14 @entity652 @entity2513
DNA aneuploidy is a biological marker of the oncogenic potential of @entity2513 . The accumulation of genetic alterations of @entity5 -related genes is also essential for @entity14 . However, it is unclear whether there is any relationship between these genetic alterations and the DNA ploidy of @entity5 cells in the progression of @entity2513 and early @entity14 . Here we have studied the DNA ploidy state and genetic alterations occurring in @entity14 using the crypt isolation technique. Crypts isolated from a total of 106 @entity14 ( @entity652 , 93; early @entity189 , 13) were examined using a combination of flow cytometric analysis of DNA content, polymerase chain reaction-microsatellite assay, and single-strand conformation polymorphism assay for evidence of chromosomal allelic imbalance (AI; 17p; 5q; 18q) or @entity876 gene mutation. In addition, we examined @entity124 ( @entity124 ) with BAT 26 primer sets. DNA multiploidy was infrequently detected in @entity2513 (15.1%), in contrast to early @entity189 (46.2%). There was a significant difference in the incidence of AI of chromosome 18q between diploid @entity652 and @entity2007 populations of multiploid @entity652 (18.1% vs 57.1%, p = 0.0043). Mutation of @entity876 was also found more frequently in @entity2007 populations of early multiploid @entity14 than in early diploid @entity14 (66.7% vs 0%, p = 0.021). @entity124 was found in only 2 of 93 @entity652 , with no @entity124 detected in early @entity14 . The two @entity124 -positive @entity652 were diploid. We subdivided multiploid @entity652 into two groups: those with a low or a high @entity894 ( @entity894 ). The incidence of genetic alterations of high- @entity894 @entity652 did not differ from those of low- @entity894 @entity652 . Allelic imbalance involving loci on chromosome 18q and mutations of @entity876 seems to be associated with the progression of diploidy to multiploidy in @entity14 . On the other hand, @entity124 may be associated with the development of some @entity5 . In addition, the incidence of genetic alterations in the @entity2513 that we examined appears to be independent of the @entity5 's @entity894 .
