Intranuclear inclusion bodies in liver of chronic @entity1956 with XXXX .

@entity1 @entity1956 @entity4620 @entity2033 @entity99
Seventy-two @entity1 with HBsAg-positive chronic @entity99 were studied for serum antibody to delta antigen (anti- @entity2033 ) by radioimmunoassay. Delta antigen (HDAg) in liver of the 72 @entity1 was also studied immunohistochemically using peroxidase-labeled anti- @entity2033 . In one of the 72 @entity1 , high titers of anti- @entity2033 and intrahepatic HDAg were detected. In this @entity1 , HDAg was localized mainly in nuclei and occasionally in the cytoplasm of hepatocytes. By immunoelectron microscopy, HDAg was observed diffusely in some hepatocytic nuclei, but their nucleoli, perichromatin and heterochromatin were devoid of HDAg. Occasionally, inclusion body-like ring-shaped or irregularly shaped aggregates of HDAg were observed in nuclei. At higher magnification, these aggregates consisted of an HDAg-positive amorphous substance and microtubular structures. In their vicinity, scattered HDAg-positive granules were also present. By conventional electron microscopy, similar inclusion bodies composed of a clustered amorphous substance and microtubular structures 10-20 nm in diameter, together with 20-30 nm irregular granules were observed in hepatocyte nuclei, but no core of Dane particles were found. These novel intranuclear structures were not observed in other @entity1 with chronic @entity1956 . It is suggested that they may represent the characteristic ultrastructure associated with @entity4620 .
