ALDH activity indicates increased tumorigenic cells, but not @entity5 stem cells, in XXXX cell lines.

@entity263 @entity5 @entity19 @entity1759 @entity915 @entity3 @entity264
BACKGROUND: Cancer stem cells (CSCs) have been shown to be a small stem cell-like cell population which appears to drive tumorigenesis, @entity5 recurrence and @entity3 . Thus, identification and characterization of CSCs may be critical to defining effective anticancer therapies. In @entity263 ( @entity263 ), the @entity1759 (+) cell population appears to have stem cell-like properties including being tumorigenic. The enzyme @entity264 dehydrogenase (ALDH) has been found to identify hematopoietic stem cells and our aim was to determine the utility of ALDH activity and @entity1759 in identifying @entity263 stem cell-like cells in @entity263 cell lines. MATERIALS AND METHODS: LNCaP cells and PC-3 cells were sorted based on their expression of @entity1759 and ALDH activity. The cell populations were investigated using colony-forming assays, invasion assays, sphere formation experiments in a non-adherent environment and 3-D Matrigel matrix culture to observe the in vitro stem-cell like properties. Different sorted cell populations were injected subcutaneously into NOD/ @entity915 @entity19 to determine the corresponding tumorigenic capacities. RESULTS: ALDH(hi) @entity1759 (+) cells exhibit a higher proliferative, clonogenic and metastatic capacity in vitro and demonstrate higher tumorigenicity capacity in vivo than did ALDH(lo) @entity1759 (-) cells. The @entity5 recapitulated the population of the original cell line. However, ALDHlo @entity1759 (-) cells were able to develop @entity5 , albeit with longer latency periods. CONCLUSION: ALDH activity and @entity1759 do not appear to identify @entity263 stem cells; however, they do indicate increased tumorigenic and metastatic potential, indicating their potential importance for further exploration.
