@entity1068 -induced depletion of lymphocytes from the mesenteric lymph nodes of C57B1/6 XXXX is associated with RNA but not DNA degradation.

@entity167 @entity19 @entity1068
The effects of @entity1068 ( @entity167 ) consumption by adult female C57B1/6 @entity19 on lymphocyte populations of the mesenteric lymph nodes (MLNs) were determined by feeding @entity19 with the Lieber-DeCarli liquid diet by a pair-feeding paradigm. Histological analysis of the MLNs of @entity167 -fed @entity19 showed a progressive loss of lymphocytes from the medullary regions at 3, 5, and 7 days after initiation of the @entity167 diet. The stromal cells in the medullary region also demonstrated a progressive alteration in stellate morphological features at times corresponding to those of loss of lymphocytes from this region. Microscopic evaluation of the follicle regions of MLNs obtained from @entity19 fed an @entity167 -containing diet showed no appreciable alterations in morphological characteristics. The number of tingible body macrophages in the germinal centers of the follicles, however, was increased after 3 days of @entity167 diet feeding and declined progressively after this time. Flow cytometric analysis of isolated lymphocytes showed a depletion of both T and B cell populations from the MLNs. In contrast to B cells, however, T cells were depleted through 7 days of @entity167 diet feeding. Total RNA isolated from the MLNs of @entity19 consuming the @entity167 -containing diet was progressively degraded. No degradation of DNA was observed. These study results establish that continuous consumption of dietary @entity167 adversely affects the cellularity of MLN, resulting in a progressive loss of lymphocytes that is associated with degradation of total RNA.
