Location of motoneurons supplying individual muscles in normal and grafted supernumerary limbs of XXXX .

@entity1238 @entity4494
The purpose of this study was to investigate innervation of transplanted supernumerary hindlimbs in the frog ( @entity1238 ). Motoneurons innervating identified muscles in normal and supernumerary limbs were located by the method of retrograde transport of HRP after intramuscular injection. In the lumbar spinal cord of normal @entity1238 , motoneurons supplying medial hindlimb muscles, which are derived from the ventral muscle mass during development, are located at the medial end of the motor column; those innervating lateral, dorsally-derived muscles, lie at the lateral end of the motor column. In animals with supernumerary limbs, motoneurons supplying the transplant usually occupied the same mediolateral position as those supplying the same muscle in the normal limb. However, the rostrocaudal location of these motor pools exhibited greater flexibility. When the transplant was innervated by a rostral nerve of the lumbar plexus, motoneurons supplying gastrocnemius could be located in a region of the spinal cord whose motoneurons do not normally innervate this muscle. There is thus no rigid requirement that gastrocnemius motoneurons be located at specific segmental levels. Motoneurons supplying gastrocnemius in the normal limb on the experimental side showed normal rostrocaudal distributions, indicating little rearrangement of these motor pools. Dorsal root @entity4494 cells labeled after HRP injection could be concentrated in a @entity4494 which normally supplies little or no innervation to the injected muscle. The location of these cells confirmed the segmental source of sensory innervation of the extra limb; i.e., there was no stray innervation. Animals with supernumerary limbs exhibited little or no increase in the number of motoneurons on the extra limb side. In contrast, dorsal root @entity4494 cell populations exhibited a large increase on the experimental side.
