XXXX -induced apoptosis in primordial germ cells of the @entity35 fetus.

@entity10740 @entity330 @entity19 @entity600 @entity331 @entity35
@entity330 ( @entity331 ) is a simple alkylating agent. It induces gene mutations in fetal primordial germ cells (PGCs), and a high incidence of @entity600 is also found in the offspring of male @entity19 treated with @entity331 at the embryonic stage. It is also reported that decreases in the fertility rate and weights of the testis and ovary were found in the offspring from dams treated with @entity331 . In this study, we analyzed the occurrence of apoptotic cell death and the expression of @entity10740 protein which is thought to play an important role in the DNA damage-induced apoptosis after administration of @entity331 to pregnant @entity35 on day 13 of gestation to obtain a clue for clarifying the toxic effect of @entity331 on PGCs. Apoptotic cells increased in PGCs in fetal gonads from 3 h after treatment. The number of apoptotic PGCs peaked at 6 h and gradually decreased towards 24 h after treatment. On the other hand, @entity10740 -positive PGCs increased from I h after treatment, prior to the induction of apoptosis. The number of @entity10740 -positive PGCs peaked at 3 h and returned to the control level at 24 h after treatment. These results suggest that @entity331 induces apoptosis in @entity35 fetal PGCs immediately after its administration to dams and excess cell death by apoptosis may have a close relation to the later occurrence of decreases in the fertility rate and gonadal weight. Moreover, a possible involvement of @entity10740 is suggested in the @entity331 -induced apoptosis in PGCs.
