Effects of locally applied enzyme inhibitors of the arachidonic acid cascade on follicle growth and intra-ovarian oocyte release in hyperstimulated XXXX .

@entity1 @entity95 @entity3009 @entity3352 @entity715
This study was conducted to examine follicle growth and intra-ovarian oocyte release in @entity95 ovaries influenced by a local application of @entity3009 formation inhibitors. While five @entity95 remained as unstimulated controls, twenty-seven mature @entity95 were hyperstimulated by pure follicle stimulating hormone (FSH) followed by @entity1 chorionic gonadotropin (hCG) at time 0. At the same time, 1 mg lonazolac (lonazolac group), @entity3352 ( @entity3352 group) or saline (so-called gonadotrophic stimulated controls) was applied into the vagina of each group. Both ovaries of each animal were evaluated morphometrically at 15, 20, 28 and 48 hr after hCG. The absolute numbers of smaller-sized preantral follicles were always higher in the gonadotrophic stimulated controls than in the unstimulated controls, the lonazolac, or the @entity3352 group. The numbers of larger-sized preantral and antral follicles showed no differences. Unlike the gonadotrophic stimulated controls and the lonazolac group, the @entity3352 group displayed an increase in its mature structures (large-sized antral and preovulatory follicles, follicle and luteinized cysts) from 20 to 28 hr after hCG. Call-Exner bodies underwent pseudomucification in preovulatory follicles. The absolute numbers of Call-Exner bodies were augmented by @entity3352 . Maturation division of oocytes began to decrease in all three treated groups between 15 and 20 hr post hCG, but the height of intra-ovarian oocyte release (IOR) appeared at 28 hr. This coincided with a striking @entity715 formation. It is assumed that: 1) lonazolac and @entity3352 inhibit early development of proliferating follicles; 2) lonazolac and @entity3352 have no effects on the maturation division of the oocyte and of IOR; 3) IOR extends over a longer period after ovulation induction in contrast to the maturation division of the oocyte; and 4) @entity715 formation is related to IOR.
