Transmission of an uncultivated Bergeyella strain from the oral cavity to amniotic fluid in a case of XXXX .

@entity1 @entity527 @entity70838 @entity5870 @entity600 @entity281
Intrauterine @entity281 is a recognized cause of @entity600 . The infectious organisms are believed to originate primarily from the vaginal tract and secondarily from other parts of the body. It is plausible that microbes in the oral cavity can be transmitted to the pregnant uterus. However, direct evidence supporting such a transmission is lacking. In this study, amniotic fluids of 34 pregnant @entity1 were examined by PCR using 16S and 23S rRNA universally conserved primers. Bacterial DNA was amplified from the only @entity1 with clinical intrauterine @entity281 and histologic necrotizing acute and chronic @entity5870 . One strain, Bergeyella sp. clone @entity70838 , was detected and was 99.7% identical to a previously reported uncultivated oral Bergeyella strain, clone AK152, at the 16S rRNA level. The same strain was detected in the @entity527 of the @entity1 but not in her vaginal tract. The 16S-23S rRNA sequence of clone @entity70838 matched exactly with the sequences amplified from the @entity1 's @entity527 . These observations suggest that the Bergeyella strain identified in the @entity1 's intrauterine @entity281 originated from the oral cavity. This is the first direct evidence of oral-utero microbial transmission. The @entity1 's periodontal health during pregnancy was unclear. She did not have detectable periodontal disease during postpartum examination. Bergeyella spp. had not been previously associated with @entity600 and were detected in @entity527 of @entity1 without clinical levels of intrauterine @entity281 . Uncultivated species may be overlooked opportunistic pathogens in @entity600 . This study sheds new light on the implication of oral bacteria in @entity600 .
