Accumulation of neutral lipids in peripheral blood mononuclear cells as a distinctive trait of Alzheimer XXXX and asymptomatic subjects at risk of disease.

@entity1 @entity179 @entity195 @entity165 @entity21279
BACKGROUND: @entity195 is the most common progressive @entity179 . In recent years, numerous progresses in the discovery of novel @entity195 molecular biomarkers in brain as well as in biological fluids have been made. Among them, those involving lipid metabolism are emerging as potential candidates. In particular, an accumulation of neutral lipids was recently found by us in skin fibroblasts from @entity195 @entity1 . Therefore, with the aim to assess whether peripheral alterations in @entity165 homeostasis might be relevant in @entity195 development and progression, in the present study we analyzed lipid metabolism in plasma and peripheral blood mononuclear cells from @entity195 @entity1 and from their first-degree relatives. METHODS: Blood samples were obtained from 93 @entity1 with probable @entity195 and from 91 of their first-degree relatives. As controls we utilized 57, cognitively normal, over-65 year-old volunteers and 113 blood donors aged 21-66 years, respectively. Data are reported as mean +/- standard error. Statistical calculations were performed using the statistical analysis software Origin 8.0 version. Data analysis was done using the Student t-test and the Pearson test. RESULTS: Data reported here show high neutral lipid levels and increased @entity21279 protein in about 85% of peripheral blood mononuclear cells freshly isolated (ex vivo) from @entity1 with probable sporadic @entity195 compared to about 7% of cognitively normal age-matched controls. A significant reduction in high density lipoprotein- @entity165 levels in plasma from @entity195 was also observed. Additionally, correlation analyses reveal a negative correlation between high density lipoprotein- @entity165 and cognitive capacity, as determined by Mini Mental State Examination, as well as between high density lipoprotein- @entity165 and neutral lipid accumulation. We observed great variability in the neutral lipid-peripheral blood mononuclear cells data and in plasma lipid analysis of the subjects enrolled as @entity195 -first-degree relatives. However, about 30% of them tend to display a peripheral metabolic @entity165 pattern similar to that exhibited by @entity195 @entity1 . CONCLUSION: We suggest that neutral lipid-peripheral blood mononuclear cells and plasma high density lipoprotein- @entity165 determinations might be of interest to outline a distinctive metabolic profile applying to both @entity195 @entity1 and asymptomatic subjects at higher risk of disease.
