LATERAL ORGAN BOUNDARIES DOMAIN( XXXX )10 interacts with SIDECAR POLLEN/ @entity71882 to control pollen development in @entity958 .

@entity958 @entity71881 @entity71882 @entity347
UNASSIGNED: During male gametophyte development in @entity958 , the microspores undergo an asymmetric division to produce a vegetative cell and a generative cell, which undergoes a second division to give rise to two sperm cells. SIDECAR @entity347 ( @entity347 ) 27 plays a key role in the asymmetric division of microspores. Here we provide molecular genetic evidence that a combinatorial role of @entity71881 with @entity71882 is crucial for male gametophyte development in @entity958 . Expression analysis, genetic transmission and pollen viability assays, and pollen development analysis demonstrated that @entity71881 plays a role in the male gametophyte function primarily at germ cell mitosis. In the mature pollen of @entity71881 and @entity71881 expressing a dominant negative version of @entity71881 , @entity71881 :SRDX, aberrant microspores such as bicellular and smaller tricelluar pollen appeared at a ratio of 10 to 15% with a correspondingly decreased ratio of normal tricellular pollen, whereas in @entity71882 mutants, 70% of the pollen was aborted. All pollen in the @entity71881 @entity71882 double mutants was aborted and severely shriveled compared with that of the single mutants, indicating that @entity71881 and @entity71882 are essential for pollen development. Gene expression and subcellular localization analyses of @entity71881 :GFP and @entity71882 :RFP during pollen development indicated that posttranscriptional and/or posttranslational controls are involved in differential accumulation and subcellular localization of @entity71881 and @entity71882 during pollen development, which may contribute in part to combinatorial and distinct roles of @entity71881 with @entity71882 in microspore development. In addition, we showed that @entity71881 and @entity71882 interact to form a heterodimer for nuclear localization. This article is protected by copyright. All rights reserved.
