Dendritic cells in synovial fluid of chronic XXXX lack @entity7938 surface expression.

@entity1 @entity32 @entity625 @entity8730 @entity2936 @entity7938 @entity20394 @entity7937 @entity10985 @entity2720 @entity2071 @entity4386
Dendritic cells (DC) act as potent primary antigen-presenting cells in many immune responses and therefore may have a role in the initiation and perpetuation of the @entity32 in chronic @entity625 . To examine their function, it is important to isolate fresh DC from @entity2720 without aberrant activation. We have developed a technique using minimal cell manipulation to isolate DC from the synovial fluid of chronic arthritic @entity1 . Using this method, DC were shown to be potent allostimulatory cells, with 63-90% of cells lacking lineage-specific markers (lin-), but positive for MHC class II molecules. Two morphologically distinct populations of these cells were identified in 10 out of 13 DC preparations. Both populations expressed @entity2936 , @entity2071 ( @entity2071 ), @entity10985 , @entity20394 and @entity7937 ( @entity7937 ), but the predominant population, which was larger and more typical of cultured blood DC, had a higher density of these antigens compared with the minor population, which were smaller and morphologically similar to lymphocytes. Two new MoAbs which label activated @entity1 blood DC, @entity8730 ( @entity8730 ) and CMRF-44, were tested. @entity8730 labelled very weakly or not at all, whereas CMRF-44 was positive on the larger cells only. Likewise, the costimulator molecule, @entity7938 ( @entity7938 ), was not detected on the surface of either synovial lin- cell population, reverse transcriptase polymerase chain reaction (RT-PCR) showed little or no @entity7938 mRNA, and no binding of the @entity4386 Ig fusion protein was found. These results suggest that synovial DC are not, despite the inflammatory environment, in a fully activated state.
