Two XXXX -binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues.

@entity1 @entity32 @entity7966 @entity535 @entity2522 @entity95 @entity6785 @entity460 @entity2757 @entity309 @entity526
Using a monoclonal antibody to macrophage migration inhibition factor ( @entity6785 ), two proteins were isolated from supernatants of Concanavalin A-stimulated @entity1 peripheral blood mononuclear cells which seem to have complexed to a third component carrying the @entity6785 activity. They are therefore designated @entity6785 -related proteins or @entity2757 and @entity7966 according to their apparent molecular weights. Partial @entity535 sequences have been determined and their cDNA have been cloned and expressed in @entity526 . Both are @entity460 -binding proteins and @entity2757 seems to be largely homologous to the @entity2757 (Dorin et al., 1987). Antisera were raised in the @entity95 against the recombinant proteins and their expression in cells and tissues studied using immunohistological techniques. The proteins are only found in blood granulocytes and monocytes. In culture the number of positive monocytes sharply increased and then declined with time, suggesting that their expression is associated with early stages of monocyte/macrophage differentiation and absent from resident macrophages in all tested tissues. In acute inflammatory reactions, e.g. @entity2522 , @entity2757 is never seen in the tissue, whereas @entity7966 is expressed by intravascular monocytes and perivascular macrophages. In contrast, in @entity32 , e.g. @entity309 , @entity2757 is also expressed by macrophages in the tissue. From this it is concluded that @entity2757 and @entity7966 are expressed sequentially at defined stages of monocyte/macrophage differentiation and that dysregulation of this process in @entity32 is mirrored by the presence of @entity2757 -positive macrophages in the tissue.
