Destruction of a translationally controlled mRNA in XXXX oocytes delays @entity1420 -induced maturation.

@entity1238 @entity1420 @entity535 @entity377 @entity73244
The maternal mRNA D7 is a moderately abundant transcript in @entity1238 whose expression is highest in, and perhaps restricted to, oogenesis and early embryogenesis. The @entity377 sequence of cloned D7 cDNA was determined and shown to have the capacity to code for a 31-kD protein. This @entity535 sequence was searched against a protein data base, and no homologous proteins were found. Antibodies directed against D7 recognize in @entity1238 embryos a soluble, cytoplasmic protein with an apparent molecular weight on SDS gels of 36,000. The @entity73244 is absent from oocytes and first begins to accumulate during oocyte maturation. Its levels are highest during the first day of embryonic development and then decrease; @entity73244 was not detected in adult tissues. D7 mRNA was selectively destroyed by injection into oocytes of antisense oligodeoxynucleotides. Analysis of injected oocytes by Northern and Western blotting showed site-specific cleavage and subsequent degradation of the D7 mRNA and the failure of the @entity73244 to accumulate during @entity1420 -induced maturation. The loss of @entity73244 affects the maturation process itself, significantly delaying the time course of germinal vesicle breakdown. Thus, D7 is a newly described protein involved in oocyte maturation.
