Cloning, expression and regulation of XXXX receptors.

@entity1 @entity3007 @entity2574 @entity22179 @entity1911 @entity1809 @entity1808 @entity10393 @entity534 @entity11082 @entity35 @entity291
Complementary DNAs for @entity1808 type 1 receptor isoforms AT1A and AT1B were cloned by expression cloning from @entity291 adrenal and @entity35 vascular smooth muscles. @entity1 @entity1809 receptor was also cloned. Seven transmembrane structures emerged. The @entity1809 type receptor interacted with more than one type of G-proteins. The ligand binding site of @entity1809 involving @entity534 167, Lys199, and @entity2574 263 has been identified by site directed mutagenesis. The regulation of the receptors occur at many stages. The isoform, @entity11082 , was also expression cloned from @entity35 @entity1911 cells. Although its ligand binding is not affected by stable @entity3007 analogs, it is a seven transmembrane domain receptor. It mediates the modulations of @entity10393 phosphatase by @entity1808 and @entity11082 specific @entity22179 . The modulation was abolished by pertussis toxin. Thus, @entity11082 belongs to a new class of angiotensin receptors with unique signalling and regulatory mechanisms. @entity1809 mediates cellular growth. Interestingly, @entity11082 expression is inversely related to the mitogenic activity of cells.
