Effect of exercise training intensity on XXXX T-regulatory cells and vaccination response.

@entity97 @entity19 @entity5 @entity10062 @entity281 @entity1961 @entity2101 @entity1729 @entity166
To understand the underlying mechanism(s) for the effect of exercise at different intensities on T cell and DNA vaccination responses, we treated @entity19 in a training protocol with regular moderate-intensity exercise (MIE) or prolonged, exhaustive @entity2101 ( @entity2101 ). After 6 weeks of training, splenocytes were isolated to evaluate cytokine expression and T-regulatory (Treg) cell proportion by RT-PCR and @entity10062 , respectively. Another set of @entity19 that completed the same training protocol were used to determine DNA vaccination responses. These @entity19 were immunized three times with @entity97 DNA vaccine at 2-week intervals and euthanized on day 14 after the last immunization. Serum and splenocytes were isolated to determine humoral and cell-mediated immunity (CMI). Results showed that @entity2101 increased anti-inflammatory cytokine expression and @entity1961 (+) CD25(+) Treg cell proportion. Further, @entity2101 decreased IFN-y expression, T-lymphocyte proliferation, and antigen-specic cytotoxic response in @entity97 DNA vaccine-immunized @entity19 . MIE did not change anti-inflammatory cytokine expression or @entity1961 (+) CD25(+) Treg cell proportion but increased pro-inflammatory cytokine expression and augmented antigen-specific CMI. Thus, MIE lower the risk of @entity5 and @entity166 through enhancing the pro-inflammatory responses. By contrast, @entity2101 might increase the risk of common @entity281 , such as @entity1729 , due to an up-regulation of @entity1961 (+) CD25(+) Treg cells and anti-inflammatory responses.
