G and D cells in XXXX antral mucosa: an immunoelectron microscopic study.

@entity35 @entity1943 @entity9113
AIM: To investigate the @entity9113 secreting cells (G cells) and the @entity1943 secreting cells (D cells) of antral mucosa in @entity35 at the ultrastructural level. METHODS: Revised immunoelectron microscopic technique was used to detect the G cells and D cells in @entity35 antral mucosa through @entity9113 and @entity1943 antibodies labeled by colloidal gold. Also the relevant quantitative analysis regarding the granular number of colloidal gold in G cells and in D cells was conducted. RESULTS: Immunological granules of colloidal gold were distributed in G cells and D cells. @entity9113 labeled golden granules or @entity1943 labeled ones presented mainly as lobation-like or island-like congeries. Most of the golden congeries were observed dissociated in cytoplasms of G cells or D cells, near the basement membrane. A few golden congeries were located in nuclei. The number of golden granules in one G cell was around 107.04 +/- 19.68 and was 83.36 +/- 17.58 in one D cell. CONCLUSION: @entity9113 secreting granules are located in cytoplasms and nuclei of G cells, and @entity1943 secreting granules both in cytoplasms and in nuclei of D cells. The number of golden granules can be quantitatively analyzed to determine the relative amount of @entity9113 secreting granules or @entity1943 secreting granules.
