Expression, subcellular localization and phosphorylation status of annexins 1 and 5 in @entity1 @entity977 and a growth hormone-secreting XXXX .

@entity1 @entity5 @entity4404 @entity189 @entity216 @entity816 @entity977 @entity563 @entity652 @entity669
OBJECTIVE: @entity216 ( @entity216 ), a 37-kDa protein, plays an important role as a mediator of glucocorticoid action in the anterior pituitary gland and has been implicated in the processes of tumorigenesis in a number of other tissues. As a prelude to examining the potential role of @entity216 in the pathophysiology of @entity5 , this study examined the expression, phosphorylation status and distribution of @entity216 and the closely related protein, @entity4404 ( @entity4404 ), in a series of @entity977 . @entity1 AND DESIGN: Forty-two @entity1 @entity977 were examined. Parallel studies were performed on normal pituitary tissue, obtained postmortem, a @entity189 and a grade 4 @entity816 . MEASUREMENTS: The tissue was processed for analysis of @entity216 mRNA and protein expression by reverse transcriptase polymerase chain reaction (RT-PCR), Western blot analysis and immunogold electron-microscopic histochemistry. Parallel measures of @entity4404 mRNA and protein were also made. RESULTS: @entity216 mRNA and protein were detected in all but three @entity652 studied; the protein was localized mainly, but not exclusively, to nonendocrine cells. @entity4404 expression was more variable and was contained within both endocrine and nonendocrine cells of these @entity5 . In comparison with the @entity652 , the @entity189 showed abundant expression of both @entity216 and @entity4404 , with intense @entity216 staining in some but not all @entity5 /endocrine cells. A @entity563 -phosphorylated species of @entity216 was detected in all @entity5 studied; by contrast, @entity669 -phosphorylated @entity216 was detected in only four @entity652 and in the @entity189 . @entity216 and @entity4404 were also expressed in abundance in the @entity816 . CONCLUSIONS: The results demonstrate expression of both @entity216 and @entity4404 in @entity1 @entity5 and thus raise the possibility that these proteins influence the growth and/or functional activity of the @entity5 .
