The response of virally infected insect cells to dissolved XXXX concentration: recombinant protein production and oxidative damage.

@entity26 @entity1800 @entity4466 @entity6738 @entity7928
The effects of dissolved @entity26 (DO) concentration on virally infected insect cells were investigated in 3-L bioreactor culture. Specifically, cultures of Spodoptera frugiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) were infected with Autographa californica multiple nucleopolyhedrovirus expressing secreted alkaline phosphatase (SEAP). Following infection at a DO concentration of 50% air saturation, the DO concentration was adjusted to a final value of either 190%, 50%, or 10% air saturation. Recombinant SEAP production, cell viability, protein @entity6738 content, and @entity4466 reactive substances (TBARS) content were monitored. The increases in protein @entity6738 and TBARS contents are taken to be indicators of protein oxidation and lipid oxidation, respectively. DO concentration was found to have no noticeable effect on SEAP production or cell viability decline in the Sf-9 cell line. In the @entity7928 cell line, cells displayed an increased peak SEAP production rate for 190% air saturation and displayed an increased rate of viability decline at increased DO concentration. Protein @entity6738 content showed no significant increase in the Sf-9 cell line by 72 h postinfection (pi) at any DO concentration but showed a twofold increase at 10% and 50% DO concentration and a threefold increase at 190% DO concentration by 72 h pi in @entity7928 cells. TBARS content was found to increase by approximately 50% in Sf-9 cells and by approximately twofold in @entity7928 cells by 72 h pi with no clear relationship to DO concentration. It is hypothesized that @entity26 uptake changes due to the @entity1800 process may bear a relation to the observed increases in protein and lipid oxidation and that lipid oxidation may play an important role in the death of virally infected insect cells.
