Procollagen 1 expression in XXXX .

@entity1 @entity320 @entity13704 @entity5 @entity1489 @entity957 @entity189 @entity1491 @entity272 @entity2281 @entity1389 @entity43852
BACKGROUND: Procollagen (PC) is secreted by fibroblasts into the extracellular matrix, where it is cleaved to form collagen. The rat anti-human @entity13704 monoclonal antibody has been reported to react with @entity1389 ( @entity1389 ), a poorly differentiated but usually benign @entity272 common in elderly @entity1 . We have studied @entity13704 staining in 50 @entity5 with @entity1389 histological features (four of which were subsequently reclassified as non- @entity1389 @entity5 ) to confirm this prior observation. In addition, we have investigated @entity13704 in other @entity1489 , particularly those with spindled cell or sclerosing/desmoplastic morphologies. METHOD: Archival material was retrieved and sections were prepared and immunostained with @entity13704 as well as a panel of antibodies, including @entity2281 and MNF-116 (cytokeratins 5, 6, 7, 8, 17, and 19). RESULTS: @entity13704 staining was strongly positive in 40 of 46 (87%) AFXs. Three AFXs displayed weak staining with @entity13704 even after repeat staining of 10 @entity5 that were initially weak. Three additional @entity5 stained with both @entity13704 and were classified as @entity1389 -like @entity957 ( @entity957 ). One @entity5 with @entity1389 -like histology was @entity13704 negative and @entity2281 positive and was classified as an @entity1389 -like @entity320 . Positive staining in @entity5 cells was observed in three of nine (33%) @entity320 , three of eight (38%) desmoplastic SCCs, zero of 10 (0%) @entity43852 , zero of 10 (0%) morpheic @entity1491 , and zero of 10 (0%) sclerosing sweat duct @entity189 . CONCLUSION: @entity13704 is a useful antibody in a diagnostic immunohistochemical panel when investigating @entity1389 and @entity1389 -like @entity5 ; however, good technical quality and careful interpretation are necessary when using a panel of antibodies, particularly to keratin and @entity2281 protein, for optimal accuracy.
