Prospective analysis of minimal bone marrow infiltration in pediatric XXXX by long-distance polymerase chain reaction for t(8;14)(q24;q32).

@entity1 @entity66 @entity41 @entity5 @entity2109 @entity7639
The chromosomal translocation t(8;14)(q24;q32) represents a characteristic marker for @entity2109 ( @entity2109 ). This translocation involves the MYC oncogene on chromosome 8 and the @entity7639 ( @entity7639 ) locus on chromosome 14. Since the translocation does not produce a fusion gene, we established a long-distance polymerase chain reaction (LD-PCR) assay that can detect the t(8;14) at the genomic level. The sensitivity of the LD-PCR was 10(-4). We used the LD-PCR assay to prospectively study 78 @entity2109 @entity1 and found a specific PCR product in 52 of them. Among the 52 positive @entity1 , we could test both the @entity5 and the bone marrow (BM) at diagnosis in 33 and determined the prevalence of @entity66 ( @entity66 ) at diagnosis. In 12/33 @entity1 , BM was positive by LD-PCR and in 10 of them we conducted a study of @entity41 ( @entity41 ). Eight out of 10 @entity1 showed a clearance of @entity41 after one cycle of chemotherapy. The only two @entity1 who did not achieve a negative @entity41 status died of disease progression. The comparative analysis of sensitivity of BM aspirate, BM biopsy and LD-PCR in t(8;14)-positive @entity1 demonstrated a superiority of the molecular method in the assessment of @entity66 . The LD-PCR for t(8;14) is an important tool to study minimal BM infiltration at diagnosis and to determine its response kinetics in @entity2109 .
