Mesenchymal stem cells ameliorate LPS-induced @entity647 through XXXX promoting alveolar fluid clearance of alveolar type II cells.

@entity32 @entity3529 @entity4024 @entity48414 @entity11943 @entity133 @entity35 @entity647
OBJECTIVE: It has been shown that Mesenchymal stem cells (MSCs) could prevent or alleviate @entity647 ( @entity647 ) when transplanted into lung; however, the mechanisms involved remains elusive. The study aimed to investigate the effect of MSCs in repairing alveolar fluid clearance (AFC) of @entity133 ( @entity133 ) cells through paracrine factors. MATERIALS AND METHODS: @entity11943 ( @entity11943 ) concentration in the supernatant of MSC culture medium was measured, and MSCs in lung after intravenous administration was traced. Next, MSCs transfected with or without @entity11943 SiRNA were injected intravenously into LPS-induced @entity647 @entity35 . Histological change and wet/dry ratio were determined. @entity133 cells were co-cultured with MSCs under different experimental situations to analyze the variation of @entity48414 subunits of @entity4024 - @entity3529 -ATPase in @entity133 cells. RESULTS: LPS-induced @entity647 was characterized by the typical inflammatory infiltrates, interalveolar septal thickening and increased wet/dry ratio. MSC administration significantly reduced the injury, while MSCs with @entity11943 knockdown did no show the same effect. In vitro study also confirmed that expressions of @entity48414 subunit were up-regulated as impaired @entity133 cells co-cultured with MSCs. Furthermore, expression of a1 subunit was down-regulated, while b1 subunit showed no apparent change as @entity133 cells were co-cultured with MSCs that were transfected with @entity11943 siRNA. CONCLUSIONS: AFC was @entity32 insult. MSCs-derived @entity11943 reduced the impaired AFC through up-regulated a1 subunit but not b1 subunit, which might provide a novel therapeutic strategy for @entity647 .
