Serological and cellular immune activity of peptidoglucomannan fractions of XXXX cell walls.

@entity1 @entity535 @entity840 @entity7553 @entity5132 @entity3913 @entity1778 @entity56 @entity413 @entity46789
A two-stage extraction of isolated cell walls of @entity5132 resulted in 45% solubilization into antigens of high molecular weight leaving a wall residue which also had antigenic properties. Ice-cold dilute alkali removed 25% of the defatted cell walls. The extract was nondialyzable, had a @entity413 -to- @entity3913 ratio of 2:3 and an @entity535 content of 7.32%, and was designated peptidoglucomannan ( @entity46789 ). An additional 26% of the walls resistant to stage I were solubilized by sonic treatment yielding a fraction having a @entity413 -to- @entity3913 ratio of 6:1, termed soluble mannoglucan (sMG). The residue after extraction and sonic treatment contained 10.9% @entity3913 , which was the insoluble mannoglucan. The gel permeation behavior of @entity46789 and sMG on BioGel A5M was similar; each contained two components, one estimated to exceed 5 x 10(6) molecular weight and a second smaller species. The soluble cell wall fractions were active in immunodiffusion and carried antigenic group specificity. Immunoelectrophoresis of @entity46789 , sMG, and mannan revealed some heterogeneity. The insoluble mannoglucan had agglutinating activity. A distinctive immunodiffusion pattern of cell wall antigens was formed with the serum of a @entity1778 @entity1 with @entity7553 . All three cell wall antigens and mannan elicited @entity56 as measured by skin-test and specific inhibition of macrophage migration. A dose of 25 mug of @entity46789 was sufficient to inhibit 89.9% migration in the peritoneal exudates of @entity840 immunized with cell walls, and 10 mug of @entity46789 inhibited 91.7% migration in @entity840 immunized with insoluble mannoglucan.
