@entity2942 enhances hippocampal Schaffer collateral- XXXX synaptic transmission through modulating intracellular @entity460 .

@entity1249 @entity2942 @entity10219 @entity460 @entity35 @entity11008
BACKGROUND: @entity2942 ( @entity10219 ), a natural product from the Chinese herb Polygala tenuifolia root, has been reported to improve cognitive function and exhibits neuroprotective effects in pharmacological studies of the central nervous system. Synaptic transmission is the essential process of brain physiological functions such as learning and memory formation, and @entity10219 has been shown to facilitate the basic synaptic transmission. HYPOTHESIS/PURPOSE: Although our previous work has demonstrated that @entity10219 is able to potentiate the basic synaptic transmission, the potential mechanism remains unclear. Here we investigated the effect of @entity10219 on the synaptic transmission and analysed the potential mechanism. We hope that these findings will contribute to explain the role of @entity10219 as a nootropic product or neuroprotective drug in the future. METHODS: Field excitatory postsynaptic potentials (fEPSPs), spontaneous excitatory postsynaptic currents (sEPSCs) and miniature spontaneous excitatory postsynaptic currents (mEPSCs) were recorded, by using in vitro field potential electrophysiology and whole-cell patch clamp techniques in acute hippocampal slices from @entity35 . RESULTS: @entity10219 perfusion significantly enhanced the slope of fEPSPs and reduced the ratio of paired-pulse facilitation. Moreover, @entity10219 increased the frequency and amplitude of sEPSCs but only improved the frequency of mEPSCs rather than amplitude in hippocampal @entity1249 pyramidal neurons. With removal of extracellular @entity460 , @entity10219 treatment also enhanced the mEPSCs frequency without affecting amplitude. Interestingly, the increase of mEPSCs frequency caused by @entity10219 was blocked by chelation of intracellular @entity460 with @entity11008 . CONCLUSION: These results indicate that @entity10219 enhances the basic synaptic transmission via stimulating presynaptic intracellular @entity460 .
