Implementation and application of a multiplex assay to detect XXXX -specific antibodies: a promising tool for assessing @entity311 transmission in Southeast Asian pre-elimination areas.

@entity311 @entity313 @entity281
BACKGROUND: Epidemiological surveillance is a key activity in @entity311 control and elimination in low-transmission and pre-elimination settings. Hence, sensitive tools for estimating @entity311 force of @entity281 are crucial. Serological markers might provide additional information in estimating force of @entity281 in low-endemic areas along with classical parasite detection methods. Serological markers can be used to estimate recent, past or present @entity311 exposure, depending on the used markers and their half-life. METHODS: An assay based on 14 Plasmodium-specific peptides, one peptide specific for @entity313 saliva protein and five Plasmodium-specific recombinant proteins was developed for the MAGPIX system, assessed for its performance, and applied on blood spots from 2000 individuals collected in the Ratanakiri Province, Cambodia. RESULTS: A significant correlation for the use of 1000 and 2000 beads/antigen/well as well as for the monoplex versus multiplex assay was observed for all antigens (p  <  0.05). For the majority of antigens, antigen-coupled beads were stable for at least 2  months. The assay was very reproducible with limited intercoupling, interplate and intraplate variability (mean RSD <15  %). Estimating seroconversion and seroreversion per antigen using reversible catalytic models and models allowing two seroconversion rates showed higher seroconversion rates in adults. CONCLUSION: The multiplex bead-based immunoassay was successfully implemented and analysis of field blood samples shows that changes detected in force of @entity281 vary according to the serological markers used. Multivariate analysis of the antibody responses and insights into the half-life of antibodies are crucial for improving the interpretation of these results and for identifying the most useful serological markers of past and recent @entity281 .
