Contribution of interleukin 17 to @entity1 cartilage degradation and synovial inflammation in XXXX .

@entity1 @entity5737 @entity571 @entity1356 @entity310 @entity29716 @entity2851 @entity2842 @entity4155 @entity687
OBJECTIVE: To compare the effect of @entity4155 , @entity1356 and @entity310 on chemokine production by @entity1 chondrocytes and synovial fibroblasts isolated from @entity1 with @entity571 ( @entity571 ). The expression of @entity1356 mRNA by @entity571 chondrocytes was also assessed, as well as the presence and expression of @entity29716 ( @entity29716 ) in @entity571 chondrocytes and synovial fibroblasts after stimulation with @entity4155 , @entity1356 and @entity310 . DESIGN: Synovial fibroblasts and chondrocytes isolated from @entity1 with @entity571 were stimulated in vitro with @entity4155 , @entity1356 or @entity310 . Supernatants were collected and immunoassayed for the presence of @entity687 , @entity2851 (CXC chemokines) and @entity2842 , @entity5737 (CC chemokines). The cells were used to detect the presence of @entity29716 and the expression of @entity29716 mRNA. Stimulated chondrocytes were also used to detect @entity1356 production and mRNA expression. RESULTS: @entity4155 upregulated the release of @entity687 and @entity2851 both by synovial fibroblasts and chondrocytes, and the release of @entity2842 only by chondrocytes. @entity4155 was a weaker stimulator than @entity1356 and @entity310 , except for @entity2851 release which was maximally upregulated by @entity1356 , less by @entity4155 and minimally by @entity310 . When compared to @entity1356 , @entity4155 was more active on chondrocytes than on fibroblasts. In chondrocytes the expression of @entity1356 mRNA was enhanced by @entity4155 and @entity310 , with a maximum level reached by @entity1356 . @entity4155 and @entity310 stimulated @entity1356 release in few subjects. Neither @entity4155 , @entity1356 nor @entity310 modulated the presence of @entity29716 and the expression of @entity29716 mRNA. CONCLUSIONS: These data suggest that @entity4155 could contribute to cartilage breakdown and synovial infiltration in @entity571 by inducing both the release of chemokines by chondrocytes and synovial fibroblasts and, in a less extent, the synthesis of @entity1356 by chondrocytes.
