Melting curve analysis of a groEL PCR fragment for the rapid genotyping of strains belonging to the @entity10263 XXXX group of species.

@entity10263 @entity2101 @entity6329 @entity6983
@entity10263 @entity6329 group (Lcs) consists of three phylogenetically closely related species ( @entity6329 , @entity6983 , and @entity10263 ), which are widely used in the dairy and probiotic industrial sectors. Strategies to easily and rapidly characterize Lcs are therefore of interest. To this aim, we developed a method according to a technique known as @entity2101 ( @entity2101 ), which was applied to a 150bp groEL gene fragment. The analysis was performed on 53 Lcs strains and 29 strains representatives of species that are commonly present in dairy and probiotic products and can be most probably co-isolated with Lcs strains. DNA amplification was obtained only from Lcs strains, demonstrating the specificity of the groEL primers designed in this study. The @entity2101 clustered Lcs strains in three groups that exactly corresponded to the species of the @entity6329 group. A following @entity2101 separated the 39 @entity6983 strains in two well distinct intraspecific groups, indicating the possible existence of at least two distinct genotypes inside the species. Nonetheless, the phenotypic characterization demonstrated that the genotypes do not correspond to the two @entity6983 , namely paracasei and tolerans. In conclusion, the melting curve analysis developed in this study is demonstrably a simple, labor-saving, and rapid strategy obtain the genotyping of a bacterial isolate and simultaneously potentially confirm its affiliation to the @entity6329 group of species. The application of this method to a larger collection of strains may validate the possibility to use the proposed @entity2101 protocol for the taxonomic discrimination of @entity6329 group of species. In general, this study suggests that @entity2101 can be a suitable technique for the genetic typization of @entity10263 strains.
