Intervention of @entity12837 ameliorates @entity1499 -induced oxidative stress and genotoxicity: A study in XXXX intestine.

@entity10740 @entity137 @entity5 @entity294 @entity2041 @entity1977 @entity12837 @entity1234 @entity1500 @entity35 @entity1499 @entity309
@entity1499 ( @entity1500 ) is an anti-metabolite, widely used in the @entity5 chemotherapy and @entity309 . However, its long-term clinical use is restricted on account of its severe intestinal @entity137 . The present study was aimed to investigate the intestinal @entity137 of @entity1500 and the possible protective effect of @entity12837 (LA) on @entity35 . @entity1500 -induced intestinal @entity137 was evaluated at the dose of 2.5mg/kg for short-term (5 days treatment) and 1mg/kg for long-term (5 days in a week for four consecutive weeks treatment) study. The possible protective effect of LA was evaluated in both short- as well as long-term study in a dose-dependent manner. @entity1500 treatment induced @entity294 and mortality in @entity35 , indicating its severe @entity137 in the target organ of investigation, i.e., intestine. Further, the intestinal @entity137 of @entity1500 was assessed by evaluating different parameters of oxidative stress, DNA damage, @entity137 as well as histological changes. Immunostaining for @entity10740 revealed higher genotoxic assault in the intestinal cells due to @entity1500 treatment. Pretreatment of @entity35 with LA led to significant decrease in the oxidative stress, DNA damage, cellular damage, inflammatory changes and apoptosis as determined by @entity1234 level, @entity2041 level, comet assay parameters, histological evaluation, immunostaining and terminal deoxynucleotidyl transferase-mediated @entity1977 nick end labeling (TUNEL) assay. In the present investigation, we report that LA pretreatment ameliorates @entity1500 -induced intestinal @entity137 in @entity35 as evident from the protection against oxidative stress, decrease in DNA damage and protection of cellular morphology as well as improvement in the stool consistency and animal survival rate.
