Mir-181b Functions as Anti-Apoptotic Gene in Post-Status Epilepticus via Modulation of XXXX and Notch Signaling Pathway.

@entity1976 @entity72268 @entity49547 @entity2134 @entity35 @entity1091 @entity159
BACKGROUND: The expression pattern and function of miRs in the @entity159 ( @entity159 ) @entity35 are still unclear. This study aimed to investigate the role and mechanism of miR-181b in the post- @entity159 @entity35 . METHODS: The @entity1091 - @entity2134 -induced @entity159 model was established in @entity35 . The expression of miR-181b in @entity35 blood and hippocampus was confirmed by RT-PCR at 24 hous and 7 days post- @entity159 . The expression of @entity72268 , @entity49547 , and @entity1976 was detected by RT-PCR and western blot. After the @entity35 model was treated with LV-rno-mir181b and LV-anti-181b-5p, the expression change of miR-181b, @entity72268 , @entity49547 , and @entity1976 was examined again. The effects of altering the expression of miR-181b on neurone cell apoptosis post- @entity159 were assessed. RESULTS: The expression of miR-181b significantly decreased both in post- @entity159 @entity35 hippocampus and peripheral blood at 24 hours and 7 days (p<0.05). @entity72268 is up-regulated, however, @entity49547 and @entity1976 are down-regulated (p<0.05). LV-miR-181b treatment induced down-regulation of @entity72268 and up-regulation of @entity49547 and @entity1976 (p<0.05). Neurone cell apoptosis decreased in miR-181b group and post- @entity159 @entity35 hippocampal (p<0.05). CONCLUSIONS: Our study showed the low-expression of miR-181b in the hippocampus in a post- @entity159 @entity35 model. MiR-181b negatively regulated @entity72268 as an anti-apoptotic gene via Notch signaling pathway.
