Stimulatory effects of insulin-like growth factors on DNA synthesis in the XXXX embryonic cornea.

@entity1 @entity267 @entity442 @entity5100 @entity735 @entity1014
10- to 12-week-old @entity1 @entity735 were microdissected so that a passage was opened between the outer environment and the anterior chamber which rendered free access of tissue culture medium to the endothelial cell monolayer. The dissected eye globes were maintained in organ culture for 24 h in the continuous presence of tritiated @entity1014 . Cross sections were cut through the whole eye globes and subjected to autoradiographic analysis in order to estimate the mitogenic response of @entity1 embryonic corneal endothelial cells to externally supplied growth factors and hormones. It was found that the corneal endothelial cells could be stimulated to initiate DNA synthesis by exposure to @entity442 ( @entity442 ). The @entity1014 -labelling index doubled after @entity442 supplementation. Northern blot analysis revealed the abundant presence of @entity5100 transcripts in the posterior eye. In contrast, the anterior portion of the eye, including the @entity267 , contains barely detectable levels of @entity5100 transcripts. @entity442 transcripts were detected in both parts of the eye at much lower concentrations than those for @entity5100 . No insulin transcripts were found. These results demonstrate that mRNA for both @entity442 and @entity5100 is present in the late first trimester eye. The observed stimulatory effects of @entity442 in organ culture suggest that local production of @entity442 and @entity5100 may stimulate cell proliferation in vivo.
