Localization of XXXX receptor alpha and beta RNA in germinal and nongerminal epithelia of the @entity4576 testis.

@entity172 @entity70719 @entity4576 @entity70718
The germinal epithelium of the anterior region of the @entity4576 testis is the site of spermatogenesis, whereas the nongerminal epithelium of the posterior region (seminal vesicle) may play a role in the regulation of seminal fluid composition. The available information indicates that @entity172 play a physiological role in the regulation of spermatogenesis and seminal fluid composition in vertebrates. However, the cellular distribution of @entity172 receptor (ER) gene expression in the teleost testis is poorly understood. Therefore, the objective of this study was to determine the presence and cellular distribution of @entity70718 and @entity70719 transcripts in germinal and nongerminal epithelia of the mature testis of @entity4576 . RT-PCR of whole-testis RNA extracts showed that @entity70718 and @entity70719 RNAs are present in the mature testis. In situ hybridization of histological sections of the germinal epithelium showed that primary spermatocytes contain little or no @entity70718 or @entity70719 RNA. However, both ER transcripts were present in secondary spermatocytes and spermatids and their levels were relatively high in mature sperm. The columnar epithelium of the seminal vesicle also contained @entity70718 and @entity70719 RNA. The ER RNAs in epithelial cells of the seminal vesicle were not evenly distributed throughout the cytoplasm but seemed to concentrate in their apical region, near the nucleus. In conclusion, @entity70718 and @entity70719 genes are coexpressed in germinal and nongerminal epithelia of the mature testis of @entity4576 and seem to be developmentally regulated in spermatocytes. These observations are consistent with the concept that @entity172 , via interaction with @entity70718 and @entity70719 , participate in the regulation of male gamete development and fertility.
