Calcium Signaling in a Genetically Engineered XXXX Pancreatic b-Cell Line.

@entity1 @entity8561 @entity9608 @entity2690 @entity4337 @entity593 @entity11243 @entity413 @entity5402 @entity9495 @entity5445
OBJECTIVES: The use of primary @entity1 b-cells for studying @entity9495 signaling is limited by the scarcity of @entity1 pancreatic islets. @entity9608 cell lines are widely used, but it is difficult to extrapolate results obtained from rodent cells to @entity1 . Recently, a genetically engineered @entity1 b-cell line EndoC-BH1 has been developed. We have examined whether the EndoC-BH1 cells could be used as a model for studying @entity9495 signaling in the b-cells. METHODS: We used microscope-based fluorometry to measure cytoplasmic-free @entity9495 concentration from @entity593 -loaded single EndoC-BH1 cells cultured on glass cover slips. @entity9495 responses to different agonists of @entity2690 secretion were studied. @entity2690 secretion was measured by radioimmunoassay. RESULTS: EndoC-BH1 cells secreted @entity2690 in response to @entity413 in a dose-dependent manner, and the secretion was enhanced by @entity5445 ( @entity5445 ). @entity413 , @entity4337 , @entity5402 , @entity8561 , and @entity11243 increased cytoplasmic-free @entity9495 concentration in the EndoC-BH1 cells. We found that @entity5445 was essential for @entity9495 response to @entity413 and @entity11243 . CONCLUSIONS: We concluded that the EndoC-BH1 cells can be used as model cells to study @entity9495 signaling and stimulus-secretion coupling in the @entity1 b-cells.
