@entity9367 suppresses proliferation, XXXX -inducible  factor-1a and c-Myc expression in @entity788 cells.

@entity788 @entity5 @entity19 @entity32246 @entity430 @entity9367 @entity519 @entity1965 @entity1611
UNASSIGNED: @entity9367 (TL) is known to suppress the proliferation of a number of @entity788 cell lines in  vitro. Marked antitumor effects were also observed in a xenograft model of @entity788 . @entity519  -inducible factor  -1a ( @entity1965 ) is highly expressed in @entity788 cells lines. The present study therefore tested the hypothesis that suppression of @entity1965 is associated with the antitumor activity of TL. Quantitative polymerase chain reaction and western blot analysis were used to determine the level of gene expression. A xenograft @entity5 model of @entity788 was established in athymic @entity19 and the @entity5 size was measured to evaluate the outcome of TL treatment. Immunohistochemistry was used to detect the expression of @entity1965 and @entity1611 ( @entity1611 ), and to assess microvessel density. Microarray was used to investigate gene expression in @entity788 cells following TL treatment. The expression of @entity1965 was shown to be reduced in pancreatic cell lines following treatment with TL, and this effect occurred in a dose  -dependent manner. In a xenograft model of @entity788 , reduced levels of @entity1965 were also observed in @entity19 that were treated with TL. Furthermore, the expression of @entity1611 , which is a direct target of @entity1965 , was also suppressed, and the microvessel density of @entity5 tissues was consequently reduced. A microarray analysis of gene expression was performed in order to investigate the potential mechanisms underlying the antitumor activity of TL. The results showed that 11 genes, including c  -Myc, @entity430 and @entity32246 , were downregulated at an early stage following treatment with TL. A recent study indicated that overexpression of c  -Myc in colon @entity5 cells promotes increased expression of @entity1965 and @entity1611 . Therefore, TL may suppress @entity1965 through a c  -Myc  -dependent mechanism, which is involved in antitumor effects in @entity19 models of @entity788 .
