microRNAs expression in XXXX and their relation to angiogenic factors.

@entity43 @entity1 @entity3499 @entity27437 @entity66 @entity7 @entity75 @entity6557 @entity12425 @entity377 @entity6378 @entity46 @entity10074
BACKGROUND: Endometriosis is a common, @entity66 in which angiogenesis may be involved in the growth of endometrium outside the uterus. microRNAs (miRNAs) are 21-22 @entity377 non-coding RNAs that regulate gene expression and play fundamental roles in biological processes. The objective of this study was to analyze several miRNAs related to angiogenesis and the angiogenic factors, @entity7 ( @entity7 ) and @entity3499 ( @entity3499 ), in @entity75 ( @entity43 , @entity6378 and rectovaginal nodule) and eutopic endometrium from @entity1 with @entity46 . METHODS: TaqMan real-time PCR was used to assess the expression of the miRNAs ( @entity27437 , -16, -17-5p, -20a, -21, -125a, -221 and -222), while @entity7 and @entity3499 mRNA were assessed by real-time PCR, with SYBR Green I and @entity7 and @entity3499 protein levels were quantified by ELISA. Included in the study were 58 @entity1 with @entity46 and 38 control @entity1 . RESULTS: In paired samples, @entity43 showed significantly lower @entity7 mRNA (P = 0.02) and protein (P = 0.002) expression than eutopic endometrium and higher expression of @entity6557 (P = 0.003) and @entity10074 (P <0.001). However, @entity43 had significantly higher expression of the angiogenic inhibitor @entity3499 and lower expression of @entity12425 than eutopic endometrium (P < 0.001). Moreover, a significant inverse correlations between @entity10074 and @entity7 protein levels (-0.267, P = 0.018) and between @entity12425 and @entity3499 protein levels (-0.260, P=0.022) were observed. Peritoneal lesions showed a significant increase in @entity7 in comparison with @entity43 (P < 0.01). CONCLUSIONS: Expression levels of miRNAs related to angiogenesis were different in eutopic endometrium from that observed in @entity43 . This could influence the expression of angiogenic factors and play a role in the pathogenesis of @entity46 .
