Fibronectin promotes survival and migration of primary neural stem cells transplanted into the traumatically injured XXXX brain.

@entity131 @entity10245 @entity146 @entity19 @entity130
Multipotential stem cells are an attractive choice for cell therapy after @entity131 ( @entity131 ), as replacement of multiple cell types may be required for functional recovery. In the present study, neural stem cells (NSCs) derived from the germinal zone of E14.5 GFP-expressing @entity19 brains were cultured as neurospheres in @entity10245 -enhanced medium. When @entity10245 was removed in vitro, NSCs expressed phenotypic markers for neurons. astrocytes, and oligodendrocytes and exhibited @entity146 in the presence of adsorbed fibronectin (FN). NSCs (10(5) cells) were transplanted into @entity19 brains 1 week after a unilateral, controlled, @entity131 (depth = 1 mm, velocity = 6 m/s, duration = 150 ms) (n = 19). NSCs were injected either directly into the @entity130 with or without an injectable FN-based scaffold [collagen I (CnI)/FN gel; n = 14] or into the striatum below the @entity130 (n = 5). At all time points examined (1 week to 3 months posttransplant), GFP+ cells were confined to the ipsilateral host brain tissue. At 1 week, cells injected into the @entity130 lined the injury penumbra while cells inserted directly into the striatum remained in or around the needle track. Striatal transplants had a lower number of surviving GFP+ cells relative to cavity injections at the 1 week time point (p < 0.01). At the longer survival times (3 weeks-3 months), 63-76% of transplanted cells migrated into the fimbria hippocampus regardless of injection site, perhaps due to cues from the degenerating hippocampus. Furthermore, cells injected into the cavity within a FN-containing matrix showed increased survival and migration at 3 weeks (p < 0.05 for both) relative to injections of cells alone. These results suggest that @entity10245 -responsive NSCs present a promising approach for cellular therapy following trauma and that the transplant location and environment may play an important role in graft survival and integration.
