Spatial-temporal modulation of CCN proteins during wound healing in XXXX skin in vivo.

@entity1 @entity31200 @entity59414 @entity4186 @entity7596 @entity44747 @entity12020
CCN proteins are important modulators of development and function of adult organs. In this study, we examined the localization and expression of the six CCN family members in normal adult @entity1 skin and during wound healing in vivo. Transcript and protein expression were studied by laser-capture microdissection-coupled real-time PCR and immunohistochemistry, respectively. Our results demonstrate that @entity7596 , @entity59414 , and @entity44747 are expressed at relatively low levels in normal @entity1 skin. @entity12020 , @entity31200 , and @entity4186 are the most highly expressed transcripts in the epidermis. @entity31200 and @entity4186 proteins are prominent in epidermal keratinocytes, whereas @entity12020 is primarily expressed in melanocytes. Differential expression within epidermal layers suggests that @entity31200 and @entity4186 are linked with keratinocyte differentiation. @entity12020 , @entity31200 and @entity4186 , are the three most highly expressed transcripts in the dermis. Their respective proteins are produced to various extents by dermal fibroblasts, blood vessels, eccrine sweat glands and hair follicles. We find that most CCN family members are temporally and specifically regulated during different phases (inflammation, proliferation, and remodeling) of partial thickness wound repair. By highlighting spatial-temporal regulations of CCN family member expression in relation to cell proliferation and differentiation, our results suggest a diverse range of functions for CCN proteins in both epidermal and dermal cells, and provides a solid reference for interpretation of future studies aimed at understanding the role of CCN proteins in @entity1 skin physiology and diseases.
