MicroRNA-211, a direct negative regulator of @entity8188 expression, inhibits XXXX cells' growth and migration.

@entity1 @entity22410 @entity0 @entity5 @entity8189 @entity8188 @entity4009
UNASSIGNED: The non-coding microRNAs (miRNAs) have tissue- and disease-specific expression patterns. Dysregulation of miRNAs has been associated with initiation and progression of oncogenesis in @entity1 . The abnormal expression of @entity8188 phosphatases detected in a number of @entity5 implies that their dysregulation is involved in malignant transformation. Using miRNA target prediction software, we found that @entity22410 could target the 3'UTR sequence of @entity8188 . To shed light on their roles of @entity22410 in @entity0 , the expression of @entity22410 was examined by real-time RT-PCR in @entity0 and normal tissues. @entity22410 is significantly downregulated in @entity0 . @entity22410 re-expression suppressed cell growth, cell cycle, migration, and invasion in @entity0 ( @entity0 ) cell line MDA-MB231. Luciferase expression from a reporter vector containing the @entity8188 -3'UTR was decreased when this construct was transfected with @entity22410 . The over-expression of @entity22410 suppressed the endogenous @entity8188 protein level in @entity0 cells. For the first time, we demonstrate that miRNA-211 is a direct negative regulator of @entity8188 expression in @entity0 cells, alters other related target proteins @entity8189 and @entity4009 , and then inhibits @entity0 cells growth, migration, and invasion and lead G2/M arrest. The transcriptional loss of @entity22410 and the resultant increase in @entity8188 expression facilitate increased genomic instability at an early stage of @entity5 development.
