Natural killer (NK) cell immunodeficiency in @entity1 with XXXX . II. Successful cloning and amplification of natural killer cells.

@entity1 @entity2212 @entity9638 @entity4755 @entity1778 @entity1199 @entity1860
Defective natural killer (NK) cell populations from @entity1 with @entity1860 ( @entity1860 ), that reacted with both @entity4755 + and B73.1+ antibodies, were obtained by a fluorescence-activated cell sorter (FACS). These fractions, along with NK fractions from normal donors which reacted with both antibodies, were expanded as bulk cultures or clones by limiting dilution, for 4 weeks in the presence of 10% @entity1199 ( @entity1199 ), @entity1 type AB plasma, and irradiated @entity1 allogeneic mononuclear cells. Successfully established clones from @entity1 with @entity1860 , with lytic activity against autologous and more differentiated neoplastic granulocytes, were generated more efficiently from B73.1+ than from @entity4755 + subsets. However, there were no significant differences among the generations of B73.1+ and @entity4755 + clones for both @entity1 and normal donors with lytic activity against NK susceptible K-562 targets. Fresh myeloblast preparations from a @entity9638 were found to be more susceptible to lysis by @entity1199 -proliferative B73.1+ and @entity4755 + clones than were fresh myelocyte preparations from a @entity2212 @entity1 , which were lytically susceptible to only B73.1+ clones. B73.1+ and @entity4755 + subsets from @entity1860 @entity1 demonstrated major histocompatibility complex nonrestricted killing, and showed the following predominant phenotypes: B73.1+T3+T8+ or B73.1+T3+T8- from B73.1+ subsets; and @entity4755 -T3+T8+ (initially @entity4755 +) from @entity4755 + subsets. In contrast, B73.1+ and @entity4755 + clones from normal donors showed the following predominant phenotypes: B73.1+T3-T8-; and @entity4755 -T3-T8- or @entity4755 -T3-T8+ (initially all @entity4755 +). Short-term in vitro @entity1199 or interferon treatment of fresh NK defective subsets from @entity1860 @entity1 resulted in minimal cytotoxic augmentation. In contrast, defective NK cells from @entity1860 @entity1 , whether @entity4755 + or B73.1+ subsets, proliferated with complete regeneration of cytolytic activity after a 3-4 week exposure to @entity1199 , but differed in phenotypic profiles as compared to those of normal donors. These observations imply that not only fresh defective NK cells but also the cytotoxically restored clones from @entity1860 @entity1 are derived from different NK subsets and may represent undifferentiated forms of NK cells that may be arrested at an early stage of development by yet unknown mechanism(s). In vitro substantiation of @entity1778 cell killing by @entity1199 -proliferative NK cell clones is encouraging and may allow for new in vivo immunotherapeutic modalities in @entity1860 @entity1 .
