In vivo osteogenic activity of bone marrow stromal stem cells transfected with Ad-GFP- XXXX .

@entity154 @entity1841 @entity19 @entity95 @entity291 @entity9040
The aim of this study was to investigate the expression of @entity9040 ( @entity9040 ) in bone marrow stromal stem cells (BMSCs) and the in vivo and in vitro osteogenic activity of BMSCs transfected with the adenovirus plasmid, Ad-GFP- @entity1841 . The Ad-GFP- @entity1841 plasmid was packaged and transfected into @entity95 BMSCs to determine the transfection rate. The alkaline phosphatase (ALP) activities of Ad-GFP- @entity1841 -transfected BMSCs (experimental group) and untransfected BMSCs (control group) were detected. In situ hybridization of type I collagen and Western blot were used to determine the @entity9040 gene and protein expressions. The transfected and untransfected BMSCs were respectively inoculated into @entity19 to observe in vivo osteogenesis. The decalcified @entity291 cancellous bone scaffold was respectively combined with transfected and untransfected BMSCs and implanted into @entity154 in @entity95 to repair the bone. The adenovirus titer was 1.2x10(10) pfu/mL. Green fluorescent protein expression appeared 48 h after transfection with the adenovirus plasmid, and the transfection rate was 71.1%. The ALP activity was higher in the experimental group than the control group at each time point after transfection. The gene and protein expressions of @entity9040 were higher in the experimental group than the control group. The positive rates of in vivo osteogenesis in the experimental and control groups were 90% and 40%, respectively. The bone defect repair effects differed markedly between the two groups. The @entity9040 gene can be highly expressed in BMSCs to successfully induce osteogenic differentiation. BMSCs can be used as seed cells for bone tissue engineering.
