The effects of short-interval postconditioning in preventing @entity63 -reperfusion injury in XXXX .

@entity35 @entity63 @entity1234 @entity5250
AIM: Testicular torsion can lead to testicular damage. During reperfusion, tissue damage is more severe. The aim of this study was to investigate the protective effect of short-interval postconditioning and determine the optimal time of reperfusion for postconditioning. MATERIALS AND METHODS: Thirty-five adult male @entity35 were divided into 5 subgroups: Sh (sham operated), @entity5250 ( @entity5250 ), PC5 (torsion + postconditioning 5 seconds), PC10 (torsion + postconditioning-10 seconds), PC20 (torsion + postconditioning 20 seconds). Torsion was created by rotating the left testis counterclockwise 1080   and the testis fixed to the scrotum with 3 sutures. Torsion was maintained for 4 hours. The testicular artery was visualized, and before detorsion of the testis, an atraumatic vessel clamp was applied to prevent reperfusion in all study groups. Then, detorsion of the testis was performed. In the @entity5250 group, the clamp was released just after detorsion; in the PC5 group, the clamp was released for 5 seconds and closed for 10 seconds (10 times); in the PC10 group, the clamp was released for 10 seconds and closed for 10 seconds (10 times); and in the PC20 group, the clamp was released for 20 seconds and closed for 10 seconds (10 times). Then, all testes were reperfused for a 1-hour period in all study groups. After this period, the @entity35 were sacrificed, and the left testes were removed and evaluated histopathologically and biochemically. The Mann-Whitney U test was used for statistical analyses. RESULTS: Tissue @entity1234 levels were 79.3    10.6, 231.7    102.3, 71.3    12.6, 73.8    13.7, and 124.3    48.0 nmol/g tissue in the Sh, @entity5250 , PC5, PC10, and PC20 groups, respectively. Tissue @entity1234 levels were significantly lower in the PC5 and PC10 groups (P < .05) compared to the other groups. However, mean histopathologic grade was lower in all postconditioning groups compared to the control group, but the difference was significant only in the PC5 group (P < .05). CONCLUSION: We conclude that short-interval postconditioning can reduce reperfusion injury in @entity63 tissue and the optimal mode of short-interval postconditioning is 5 seconds   10 times. This technique seems easily applicable, and a similar technique may be used during testicular surgery.
