Cigarette smoke inhibits the growth of lung fibroblasts from patients with XXXX .

@entity1149 @entity2 @entity1981 @entity2542
OBJECTIVE: To investigate the proliferative characteristics of fibroblasts cultured from emphysematous lungs. METHODS: Fibroblasts were isolated and cultured from emphysematous lungs surgically resected as a consequence of lung volume reduction surgery and from non-cancerous areas of lungs obtained following resection for @entity2 as a non-emphysematous control. Their proliferative activities were compared, as was the effect of @entity1981 ( @entity1981 ) on fibroblast proliferation. After incubation with various concentrations of @entity1981 , cells were harvested and cell numbers were counted. Growth curves were constructed and doubling time was calculated to compare the proliferative activity of fibroblasts from emphysematous and @entity1149 . RESULTS: The proliferation of fibroblasts from emphysematous lungs cultured with normal medium appeared suppressed compared with @entity1149 . The addition of @entity1981 to the medium induced more obvious growth inhibition in emphysematous lung fibroblasts. The growth of both fibroblast cultures was inhibited by @entity1981 and this appeared to be concentration dependent. CONCLUSIONS: Fibroblasts from emphysematous lungs had lower proliferative activity and were more susceptible to cigarette smoke than those from @entity1149 . Cigarette smoke may play a critical role in the development and deterioration of @entity2542 by suppressing the growth of lung fibroblasts.
