Effect of prevascularization on in  vivo vascularization of XXXX /fibrin scaffolds.

@entity1 @entity49474 @entity19 @entity2279 @entity2848 @entity466
UNASSIGNED: The importance of vascularization in the field of bone tissue engineering has been established by previous studies. The present work proposes a novel @entity49474 ( @entity2848 )/fibrin composite scaffold for the development of vascularized neobone tissue. The effect of prevascularization (i.e., in  vitro pre-culture prior to implantation) with @entity1 mesenchymal stem cells (hMSCs) and @entity1 umbilical vein endothelial cells (HUVECs) on in  vivo vascularization of scaffolds was determined. Five conditions were studied: no pre-culture (NP), 1 week pre-culture (1P), 2 week pre-culture (2P), 3 week pre-culture (3P), and scaffolds without cells (control, C). Scaffolds were implanted subcutaneously in a @entity466 ( @entity466 ) @entity19 model for 9 days. During in  vitro studies, @entity2279 staining showed a significant increase in vascular network area over 3 weeks of culture. Vascular density was significantly higher in  vivo when comparing the NP and 3P groups. Immunohistochemical staining of @entity1 @entity2279 expression indicated spreading of vascular networks with increasing pre-culture time. These vascular networks were perfused with @entity19 blood indicated by perfused lectin staining in @entity1 @entity2279 positive vessels. Our results demonstrate that in  vitro prevascularization supports in  vivo vascularization in @entity2848 /fibrin scaffolds.
