Morphological and functional recovery of XXXX small intestine following localized @entity221 .

@entity4 @entity16 @entity3209 @entity35 @entity221 @entity413
Structural and functional changes in the @entity35 small intestine following localized @entity221 were examined. In anaesthetized male @entity35 a 10 cm segment of mid-small intestine was temporarily exteriorized, suspended in a cup containing Krebs-Ringer solution, and either heated at 43.5 degrees C or sham-heated at 38 degrees C for 45 min. The intestinal segments were studied 1, 4, 7, 21 and 42 days later by histopathological examination, determination of wet weight, dry weight and gross segment area, and by measuring absorption of 15 mM @entity413 containing @entity3209 -labelled @entity413 as a tracer. Intestinal @entity413 transport was assessed by two different techniques: the everted sac method (in vitro) and luminal perfusion-recirculation (in vivo). After 1 day, heated intestinal segments exhibited marked @entity16 , consisting of loss of epithelial cells and destruction of villi. Re-epithelialization had occurred by day 4, but mucosal architecture remained abnormal throughout the observation period. @entity221 caused significant thickening of the intestinal wall: at 4 days the thickening was due to @entity4 , whereas at 42 days tissue mass per cm2 in heated segments had increased by approximately 53 per cent compared with sham-heated control segments. At 1 day, net @entity413 transport in vitro in heated segments was reduced to 20 per cent and the serosal/mucosal concentration ratio to 57 per cent of that of control segments. In vivo, @entity413 transport in heated intestine at 4 days was 45 per cent of that of controls. From 4 days on, @entity413 transport improved gradually, and at 42 days there was no significant difference between heated and sham-heated animals.(ABSTRACT TRUNCATED AT 250 WORDS)
