Neuroprotection by XXXX against @entity1341 anesthesia induced @entity137 involves modulation of @entity13959 / @entity584 / @entity983 signalling in N2a and SH-SY5Y cells.

@entity137 @entity589 @entity588 @entity13959 @entity4404 @entity1836 @entity3089 @entity3090 @entity584 @entity1341 @entity98 @entity15418 @entity9459 @entity158 @entity521 @entity983 @entity6346 @entity1835 @entity2359
UNASSIGNED: @entity1341 , an @entity6346 type long-acting local anaesthetic is commonly employed for epidural anesthesia and as well for nerve blockades. However, studies have shown @entity2359 following local administration of @entity1341 raising concerns over the use of the drug. Compounds that could minimize or inhibit toxic effects of @entity1341 are of high value in operative settings and in @entity158 management. The present study aims to investigate if @entity3089 ( @entity3090 ) could inhibit or prevent @entity1341 @entity137 in @entity521 cells (N2a and SH-SY5Y). The viability of N2a and SH-SY5Y cells following exposure to @entity3090 (10-50   M) were assessed by @entity98 assay and @entity4404 /PI staining. The influence of @entity3090 on ROS generation was determined. The expression of apoptotic cascade proteins ( @entity15418 , @entity1836 , @entity1835 , @entity589 , @entity588 ) and @entity584 ( @entity584 , p- @entity584 , @entity9459 , p- @entity9459 , @entity983 ) were analyzed by western blotting. @entity3090 improved the viability of the cells and inhibited apoptosis by potentially decreasing the expression of caspases and pro-apoptotic proteins. @entity1341 induced ROS generations were reduced on @entity3090 exposure. @entity3090 significantly promoted the phosphorylation of @entity584 and @entity9459 and down-regulated @entity983 , thus activating @entity13959 / @entity584 signalling. @entity3090 effectively improved the cell viability and inhibited apoptosis of N2a and SH-SY5Y cells via suppression of ROS generation and modulation of PI3K/ @entity584 signalling cascade.
