Rhoptry-associated protein (rap-1) genes in the @entity742 pathogen XXXX sp. Xinjiang: Multiple transcribed copies differing by 3' end repeated sequences.

@entity1522 @entity70570 @entity742 @entity743 @entity4334 @entity70571
UNASSIGNED: @entity742 babesiosis occurs mainly in tropical and subtropical areas. The @entity742 parasite @entity743 sp. Xinjiang is widespread in China, and our goal is to characterize rap-1 (rhoptry-associated protein 1) gene diversity and expression as a first step of a long term goal aiming at developing a recombinant subunit vaccine. Seven different @entity70570 genes were amplified in @entity743 sp. Xinjiang, using degenerate primers designed from conserved motifs. @entity70571 and rap-1c gene types could not be identified. In all seven @entity70570 genes, the 5' regions exhibited identical sequences over 936nt, and the 3' regions differed at 28 positions over 147nt, defining two types of genes designated a and b. The remaining 3' part varied from 72 to 360nt in length, depending on the gene. This region consists of a succession of two to ten 36nt repeats, which explains the size differences. Even if the @entity4334 sequences varied, 6 repeats encoded the same stretch of @entity1522 . Transcription of at least four a and two b genes was demonstrated by standard RT-PCR.
