Relaxin enhances the collagenolytic activity and in vitro invasiveness by upregulating matrix metalloproteinases in @entity1 XXXX cells.

@entity1 @entity1552 @entity3334 @entity7175 @entity1476 @entity5 @entity22078 @entity1550 @entity1551 @entity2843 @entity259
In this study, we identified differential expression of immunoreactive @entity1476 ( @entity1476 )/gelatinase A, membrane-anchored @entity1551 / @entity1551 , and @entity1 @entity7175 ( @entity7175 ) in @entity1 benign and malignant thyroid tissues. @entity1476 and @entity1551 were detected in the majority of @entity259 tissues and colocalized with @entity7175 -positive cells. @entity1476 was mostly absent in goiter tissues and, similar to @entity7175 , may serve as a marker for @entity259 . @entity1476 and @entity1551 were identified as novel @entity7175 targets. @entity7175 caused a significant downregulation of @entity1552 ( @entity1552 ) 3 protein levels but did not change the expression levels of @entity1550 , and @entity2843 , @entity1552 , and @entity22078 in @entity1 @entity259 cells. @entity7175 failed to affect the expression of @entity3334 , 3, 8, and 9 in the @entity259 cells investigated. Stable @entity7175 transfectants secreted enhanced levels of bioactive @entity1476 which contributed to the increased collagenolytic activity and in vitro invasiveness into collagen matrix by @entity1 @entity259 cells. Three-dimensional reconstitution of confocal fluorescent microscopy images revealed larger-sized invadopodia, with intense @entity1551 accumulation at the leading migrating edge in @entity7175 transfectants when compared with enhanced green fluorescent protein clones. In @entity7175 transfectants actin stress fibers contributed to pseudopodia formation. In conclusion, enhanced @entity5 cell invasion by @entity7175 involves the formation of @entity1551 -enriched invadopodia that lead to increased collagenolytic cell invasion by @entity1 @entity259 cells.
