Amelioration of Chemotherapy-Induced Intestinal XXXX by Orally Administered Probiotics in a @entity19 Model.

@entity1 @entity19 @entity635 @entity294 @entity33 @entity16 @entity777 @entity9206 @entity1335 @entity637 @entity1487
BACKGROUND AND AIMS: Intestinal @entity33 is a frequently encountered side effect in oncology @entity1 undergoing chemotherapy. No well-established or up to date therapeutic strategies are available. To study a novel way to alleviate @entity33 , we investigate the effects and safety of probiotic supplementation in ameliorating @entity1487 -induced @entity33 in a @entity19 model. METHODS: Seventy-two @entity19 were injected saline or @entity1487 ( @entity1487 ) intraperitoneally daily. @entity19 were either orally administrated daily saline, probiotic suspension of @entity9206 casei variety rhamnosus (Lcr35) or @entity9206 and @entity1335 (LaBi). @entity294 score, pro-inflammatory cytokines serum levels, intestinal villus height and crypt depth and total RNA from tissue were assessed. Samples of blood, liver and spleen tissues were assessed for translocation. RESULTS: Marked diarrhea developed in the @entity1487 groups but was attenuated after oral Lcr35 and LaBi administrations. @entity294 scores decreased significantly from 2.64 to 1.45 and 0.80, respectively (P<0.001). Those @entity19 in @entity1487 groups had significantly higher proinflammatory cytokine levels ( @entity637 : 234.80 vs. 29.10, P<0.001, @entity777 : 25.13 vs. 7.43, P<0.001, IFN-y: 22.07 vs. 17.06, P = 0.137). A repairing of @entity16 was observed following probiotics administration. We also found @entity637 , @entity635 and @entity777 mRNA expressions were up-regulated in @entity33 tissues following @entity1487 treatment ( @entity637 : 4.35 vs. 1.18, @entity635 : 2.29 vs. 1.07, @entity777 : 1.49 vs. 1.02) and that probiotics treatment suppressed this up-regulation (P<0.05). No bacterial translocation was found in this study. CONCLUSIONS: In conclusion, our results show that oral administration of probiotics Lcr35 and LaBi can ameliorate chemotherapy-induced @entity33 in a @entity19 model. This suggests probiotics may serve as an alternative therapeutic strategy for the prevention or management of chemotherapy-induced @entity33 in the future.
