Conditioned medium of dental pulp cells stimulated by Chinese propolis show neuroprotection and XXXX extension in vitro.

@entity1 @entity2136 @entity527 @entity274 @entity33311 @entity2425 @entity179 @entity521 @entity35
UNASSIGNED: The purpose of this study was to clarify the effect of Chinese propolis on the expression level of neurotrophic factors in dental pulp cells (DPCs). We also investigated that the effects of the conditioned medium (CM) of DPCs stimulated by the propolis against oxidative and endoplasmic reticulum (ER) stresses in @entity1 @entity521 SH-SY5Y cells, and on @entity527 extensions in @entity35 adrenal pheochromocytoma PC12 cells. To investigate the effect of the propolis on the levels of neurotrophic factors in DPCs, we performed a qRT-PCR experiment. As results, NGF, but not @entity2136 and NT-3, in DPCs was significantly elevated by the propolis in a concentration-dependent manner. @entity274 -induced cell death was significantly inhibited by the treatment with the @entity33311 . In addition, the treatment with the propolis-stimulated @entity33311 had a more protective effect than that with the @entity33311 . We also examine the effect of the propolis-stimulated @entity33311 against a @entity2425 -induced ER stress. The treatment with the propolis-stimulated CM as well as the @entity33311 significantly inhibited @entity2425 -induced cell death. Moreover, the treatment with the propolis-stimulated @entity33311 significantly induced @entity527 outgrowth from PC12 cells than that with the @entity33311 . These results suggest that the @entity33311 as well as DPCs will be an efficient source of new treatments for @entity179 and that the propolis promote the advantage of the @entity33311 via producing neurotrophic factors.
