Molecular events in the progression of recurrent XXXX to @entity189 .

@entity1 @entity1724 @entity986 @entity996 @entity1185 @entity957 @entity189 @entity876 @entity1544 @entity1723 @entity358 @entity1141 @entity1082 @entity994
CONTEXT: Identification of the type of @entity1082 ) by polymerase chain reaction and sequencing to determine coinfection or superinfection (by more than 1 HPV type) and other molecular events have not been reported in a series of @entity1 exhibiting the morphologic spectrum of recurrent @entity1724 progressing to @entity189 . DESIGN: Four cases of @entity1723 recurrent @entity1724 progressing to @entity189 (no history of smoking or irradiation in 2 cases) were studied. Morphologically distinct foci ( @entity1185 , @entity358 , @entity1544 subjacent to @entity189 , and @entity957 ) were subjected to laser capture microdissection and polymerase chain reaction amplification using general primers in addition to type-specific primers for HPV types 16 and 18. Direct sequencing of polymerase chain reaction products identified the type of HPV. The tissue sections were immunostained using antibodies to @entity876 , @entity996 , @entity994 ( @entity994 ), and @entity986 proteins with a semiquantitative assessment. RESULTS: @entity1082 11 was the only type of HPV identified in all lesions of all cases associated with recurrent @entity1724 . There was a marked increase in @entity876 protein expression in foci of @entity1141 and @entity189 as compared to @entity1185 and @entity358 . An inverse correlation between @entity876 and @entity994 ( @entity994 ) protein expression was noted in all lesions. @entity996 protein expression increased from the benign to the malignant end of the spectrum. @entity986 protein was expressed in all lesions. CONCLUSIONS: Infection by HPV-11 may be an early event associated with progression of recurrent @entity1724 to @entity189 . Increased expression of @entity876 and @entity996 proteins and a reduced expression of @entity994 ( @entity994 ) protein appear to be significant subsequent events.
