Flow cytometric analysis of the response of the R3327-G XXXX prostatic @entity420 .

@entity263 @entity5 @entity35 @entity4742 @entity420
The technique of flow cytometric DNA histogram analysis (FCM) shows there to be two distinct cell populations (diploid vs aneuploid) in the poorly differentiated R3327-G @entity35 prostatic @entity420 . The following study compares @entity5 weight measurements with several FCM computer-based methods designed to determine rapidly the proliferative status of @entity5 . Hypophysectomy, bilateral adrenalectomy, orchiectomy, sham operations, or @entity4742 treatments were initiated when the @entity5 were palpable (day 21) and continued until the @entity5 were excised (day 52). Hypophysectomy, orchiectomy, adrenalectomy, and @entity4742 treatments all resulted in significant inhibition by @entity5 weight. Quantitation of the percentage of mid-S phase aneuploid cells by summation gave the best correlation with @entity5 weight. @entity5 grown in hypophysectomized, orchiectomized, adrenalectomized, or @entity4742 -treated animals showed a significant reduction in the proportion of mid-S phase cells as compared with controls. The calculation of the percentage of all aneuploid cells was significantly reduced in hypophysectomy, orchiectomy, and @entity4742 -treated animals. However, @entity5 grown in adrenalectomized animals were not significantly different from controls by this method. Adrenalectomy was found to be the least effective form of therapy, and this was reflected in all of the parameters measured. These data show that FCM analysis may be useful in the quantitation of @entity263 response to therapy.
