In Vitro Evaluation of Reversible and Time-Dependent Inhibitory Effects of Kalanchoe Crenata on XXXX and @entity5322 Activity.

@entity1 @entity6 @entity4433 @entity235 @entity565 @entity1042 @entity5322 @entity645 @entity102 @entity281 @entity791
UNASSIGNED: Kalanchoe crenata popularly known as " @entity235 's liver" is used in most African countries for the treatment of @entity102 such as @entity6 , @entity565 and @entity645 / @entity791 related @entity281 . The evaluation of K. crenata for herb-drug interactions has not been reported. This study therefore aims to evaluate the risk of K. crenata for herb-drug interaction in vitro. Crude @entity1042 and fractions of K. crenata were incubated and preincubated with recombinant @entity1 @entity4433 and @entity5322 . Comparative studies were conducted in both @entity1 liver microsomes and recombinant @entity1 CYP to ascertain the inhibition profile of the crude extract and the various fractions. The cocktail approach of recombinant @entity1 CYPs was conducted to confirm the inhibition potential of the fractions in the presence of other CYPs. The results showed significant time-dependent inhibition of tested samples on @entity5322 with crude @entity1042 (39KC), fractions 45A, 45B and 45D given IC50 fold decrease of 3.29, 2.26, 1.91 and 1.49 respective. Time dependent kinetic assessment of 39KC and 45D showed KI and kinact values for 39KC as 1.77 g/mL and 0.091 min-1 while that of 45D were 6.45  g/mL and 0.024 min-1 respectively. Determination of kinact based on IC50 calculations yielded 0.015 and 0.04 min-1 for 39KC and 45D respectively. Cocktail approach exhibited fold decreases in IC50 for all test fractions on @entity5322 within the ranges of 2.10 - 4.10. At least one phytoconstituent in the crude @entity1042 extract of Kalanchoe crenata is a reversible and time-dependent inhibitor of @entity5322 .
