Cell volume regulation in XXXX intestinal mucosa.

@entity2656 @entity519 @entity2865 @entity7832 @entity2083 @entity597
1. Ion and water content of @entity2083 intestinal mucosa, stripped free from muscular layers were measured under various incubation conditions. 2. Ouabain induces an increase in cation content that is electrically compensated for by @entity2865 . The increase in solute content is accompanied by an increase in water content. 3. When extracellular @entity2865 is partially replaced by @entity597 , @entity7832 does induce cell shrinkage. 4. @entity519 induces a rapid increase in cell volume that is restored by oxygenation of the incubation solution. Ouabain prevents the restoration of volume. 5. It is concluded that the classical ouabain-sensitive Na/K pump participates in the maintenance of cellular volume. We suggest that the constancy in volume after ouabain poisoning as is reported for many tissues might be due to a low @entity2865 conductance of its membranes. 6. Anisotonic media (range: 0.6-1.2 isotonicity), made by variation on @entity2656 concentration, induce changes in cell water content that deviates from the simplified van't Hoff equation by about 10%. No change in water content after the initial increase was found. 7. We conclude that @entity2083 enterocytes do not possess a mechanism for rapid volume readjustment.
