[The impact of autophagy on proliferation of HEL cells and hematopoietic cells of polycythemia vera @entity1 with XXXX @entity4017 mutation].

@entity1 @entity8836 @entity4016 @entity3446 @entity4406 @entity21684 @entity4017
OBJECTIVE: To detect the activity of autophagy and explore the impact on survival and proliferation of HEL cells and hematopoietic cells of @entity4406    @entity1 with @entity4016 @entity4017 mutation. METHODS: Flow cytometry, AO staining and Western blot methods were used to detect the autophagy activity and the expression of @entity21684 -    protein of @entity4016 @entity4017 + HEL cells and hematopoietic cells of 12 newly diagnosed @entity4406 @entity1 with @entity4016 @entity4017 mutation. HEL cells and bone marrow cells of 3 @entity4406 @entity1 were treated with @entity3446 or @entity8836 to induce and inhibit autophagy, respectively. CellTiter Glo(R) method was used to detect the proliferation activity of cells. RESULTS: There was higher level of mean @entity21684 -    fluorescence intensity in HEL cells (159 389  29 001) than that in K562 cells (96 047  24 134) (P=0.044). The formation of autophagosome in HEL cells is more than that in K562 cells detected by microscope. What's more, the level of mean @entity21684 -    fluorescence intensity in 12 @entity4406 @entity1 ' myeloid cells (92 842  4 250) was higher than that of 15 healthy volunteers (86 633  2 504) (P=0.001). The expression of @entity21684 -    protein was higher in @entity4406 @entity1 ' peripheral blood cells than that in healthy volunteers detected by Western blot. After treated with @entity3446 12, 24, 48 h, the activity of autophagy in HEL cells and bone marrow cells of 3 @entity4406 @entity1 were increased and the proliferation activity was higher than the control group, the proliferation activity at 48 h were (101 413  3 720), (18 744  1 015), respectively. However, after treated with @entity8836 12, 24, 48 h, the activity of autophagy was decreased and the proliferation activity was lower than the control group, the proliferation activity at 48 h were (5 732  166), (5 371  56), respectively. CONCLUSION: There is high basical activity of autophagy in @entity4016 @entity4017 + HEL cells and hematopoietic cells of @entity4406 @entity1 with @entity4016 @entity4017 mutation. Up-regulated autophagy promotes proliferation of @entity4016 @entity4017    HEL cells and bone marrow cells of @entity4406 @entity1 with @entity4016 @entity4017 mutation. Decreased autophagy inhibits proliferation of @entity4016 @entity4017 + HEL cells and bone marrow cells of @entity4406 @entity1 with @entity4016 @entity4017 mutation.
