Loss of insulin response to XXXX but not @entity369 during the development of autoimmune diabetes in BB/ @entity35 : relationships to islet volume and @entity413 transport rate.

@entity35 @entity369 @entity413 @entity6
The insulin and glucagon responses to 10 mM @entity413 and 10 mM @entity369 were studied in pancreata isolated from nondiabetic @entity6 -resistant BB/ @entity35 at 60, 80, and 140 days of age and in @entity6 BB/ @entity35 on the 1st and 14th days of their @entity6 . In the former group the insulin response to @entity413 declined progressively with age (r = -0.575; P less than 0.01) and at 140 days was significantly below age-matched @entity6 -resistant controls (P less than 0.05). The insulin response to @entity369 did not decline with age in either group. For @entity6 @entity35 , on the first day of the @entity6 , the insulin response to @entity413 was absent but the response to @entity369 did not differ from nondiabetic controls. On day 14 responses to @entity413 and @entity369 were both absent. The glucagon response to @entity369 showed no trend despite a decline in baseline glucagon secretion. Endocrine tissue in nondiabetic @entity6 -prone @entity35 made up 0.8 +/- 0.2% of the pancreas at 60 days of age and 0.52 +/- 0.22% at 140 days of age; the latter was significantly less than in 140-day-old @entity6 -resistant controls (P less than 0.05). In @entity6 @entity35 on the 1st and 14th days of @entity6 endocrine tissue was 0.2 +/- 0.1% and 0.07 +/- 0.02%, respectively. The @entity413 transport rate in islets isolated on the first day of @entity6 was profoundly reduced compared to age-matched nondiabetic @entity6 -prone controls. Thus, a population of @entity369 -responsive, @entity413 -unresponsive islets with low @entity413 transport rates is present at the onset of overt @entity6 in BB/ @entity35 .
