Comparison of two techniques for quantitation of encysted cyathostome larvae in the XXXX .

@entity98 @entity1641 @entity5828
Haustral portions of intestine of 6 @entity1641 were isolated by excising the taeniae coli from the cecum and the ventral colon. Uniform 5-cm X 5-cm sections were cut from the haustra and were illuminated from the serosal side with a strong light source (mural transillumination). Cyathostome larvae encysted in the mucosa and submucosa were observed at 15 X magnification and counted. Two separate counts of the larvae in 80 replicates of tissue by the mural transillumination technique ( @entity98 ) revealed no significant (P less than 0.05) difference between sample means. Larvae in tissue sections were counted in situ by @entity98 , and the mucosal scrapings of the tissue sections were digested in pepsin and @entity5828 to determine larval yields for comparison with the @entity98 counts. Numbers of larvae recovered by pepsin and @entity5828 digestion for 3 and 6 hours were significantly (P less than 0.01) lower than were numbers originally determined by @entity98 . Larvae recovered by tissue digestion for 3 or 6 hours were examined individually and given objective scores for morphologic damage. Distribution of scores was time-dependent; increased damage to larvae was associated with a longer time of digestion. Individual 4th-stage cyathostome larvae were dissected from cysts in the large intestinal wall and were incubated in water, 0.9% saline solution, 1.1% @entity5828 , or pepsin (7,000 U of activity/ml). Significantly fewer (P less than 0.05) larvae were recovered from all solutions after 3 and 6 hours. The proportion of dissected larvae that were given high scores after exposure to pepsin was significantly (P less than 0.01) greater than were those held in @entity5828 , saline solution, or water for both periods.
