High resolution proton nuclear magnetic resonance studies of XXXX cerebrospinal fluid.

@entity1 @entity5693 @entity1160 @entity10488 @entity1792 @entity4008 @entity2228 @entity6 @entity598 @entity3793 @entity1853 @entity2774 @entity1578 @entity5189 @entity880 @entity4857 @entity815 @entity669 @entity493 @entity413
One- and two-dimensional (correlated shift spectroscopy) high resolution proton n.m.r. spectra of @entity1 cerebrospinal fluid (CSF) are reported. The merits of water suppression by freeze drying or irradiation, and spectral simplification by spin-echo methods, are discussed. Well-resolved resonances for a range of low molecular weight metabolites such as @entity880 , @entity10488 , @entity493 , @entity598 , @entity1792 , @entity413 , @entity4857 and @entity5189 were observed. Resonances for @entity1160 were observed only from freeze dried samples. Concentrations determined by n.m.r. were in reasonable agreement with those from conventional methods. The n.m.r. spectra of CSF were related to the clinical conditions of the subjects. No resonances for @entity1792 were present in spectra of CSF from subjects (three @entity1 ) with @entity4008 ; high @entity880 and lowered @entity413 levels were observed. Strong resonances for @entity413 and @entity2228 were observed for mildly @entity6 subjects. Both the aromatic and the aliphatic regions of the CSF spectra from subjects suffering from @entity1578 contained distinctive features characteristic for @entity5693 : Intense resonances for @entity880 , @entity493 , @entity4857 , @entity1853 , @entity669 , @entity815 and @entity3793 . In some cases @entity2774 was also present, which does not appear to have been reported previously. The two-dimensional spectrum suggested the presence of abnormally high levels of a number of endogenous metabolites. Such assignments were not possible using one-dimensional spectra alone because of signal overlap.
