MoSET1 (Histone H3K4 Methyltransferase in XXXX ) Regulates Global Gene Expression during Infection-Related Morphogenesis.

@entity268 @entity71444 @entity2247 @entity281 @entity376 @entity8812 @entity3505
Here we report the genetic analyses of histone @entity376 methyltransferase (KMT) genes in the phytopathogenic fungus @entity8812 . Eight putative @entity8812 KMT genes were targeted for gene disruption by homologous recombination. Phenotypic assays revealed that the eight KMTs were involved in various @entity281 processes at varying degrees. Moset1 disruptants (  moset1) impaired in histone H3 @entity376 4 methylation (H3K4me) showed the most severe defects in @entity281 -related morphogenesis, including conidiation and appressorium formation. Consequently,   moset1 lost pathogenicity on @entity3505 host plants, thus indicating that H3K4me is an important epigenetic mark for @entity281 -related gene expression in @entity8812 . Interestingly, appressorium formation was greatly restored in the   moset1 mutants by exogenous addition of @entity268 or of the cutin monomer, @entity71444 . The   moset1 mutants were still infectious on the super-susceptible @entity2247 cultivar Nigrate. These results suggested that MoSET1 plays roles in various aspects of @entity281 , including signal perception and overcoming host-specific resistance. However, since   moset1 was also impaired in vegetative growth, the impact of MoSET1 on gene regulation was not @entity281 specific. ChIP-seq analysis of H3K4 di- and tri-methylation (H3K4me2/me3) and MoSET1 protein during @entity281 -related morphogenesis, together with RNA-seq analysis of the   moset1 mutant, led to the following conclusions: 1) Approximately 5% of @entity8812 genes showed significant changes in H3K4-me2 or -me3 abundance during @entity281 -related morphogenesis. 2) In general, H3K4-me2 and -me3 abundance was positively associated with active transcription. 3) Lack of MoSET1 methyltransferase, however, resulted in up-regulation of a significant portion of the @entity8812 genes in the vegetative mycelia (1,491 genes), and during @entity281 -related morphogenesis (1,385 genes), indicating that MoSET1 has a role in gene repression either directly or more likely indirectly. 4) Among the 4,077 differentially expressed genes (DEGs) between mycelia and germination tubes, 1,201 and 882 genes were up- and down-regulated, respectively, in a Moset1-dependent manner. 5) The Moset1-dependent DEGs were enriched in several gene categories such as signal transduction, transport, RNA processing, and translation.
