JAK-2 @entity4017 Mutational Analysis in Primary Idiopathic XXXX : Experience from Southern Pakistan.

@entity1 @entity82 @entity146 @entity5 @entity4016 @entity4018 @entity381 @entity4017
BACKGROUND: @entity4018 ( @entity4018 ) is a clonal Ph-chromosome negative @entity5 characterized by dysregulated kinase signaling and release of abnormal cytokines. In the recent past, following @entity4016 @entity4017 mutation invention, important revolution has been made in the molecular diagnostic biology of this disease. The rational of this study was to determine the mutational status of @entity4016 @entity4017 in Pakistan @entity1 with @entity4018 . MATERIALS AND METHODS: In this cross sectional study, 20 @entity1 with @entity4018 were enrolled from January 2011 to December 2014. Diagnosis was based on WHO criteria for @entity4018 . All @entity1 were screened for G-T point mutation ( @entity4017 ) in the @entity4016 gene on chromosome 9 by allele specific PCR. RESULTS: The mean age was 57.9    16.5years. The male to female ratio was 3:1. The frequency of @entity4016 @entity4017 positivity in our @entity4018 @entity1 was found to be 55%. Positive correlations of @entity4016 @entity4017 mutation were established with high TLC count, raised LDH and marked @entity381 (P<0.05). No correlation of @entity4016 @entity4017 could be established with age and gender (P>0.05). CONCLUSIONS: The @entity4016 @entity4017 mutation frequency in our @entity4018 @entity1 was similar to those reported previously. In our hands @entity4016 @entity4017 mutated @entity1 expressed an @entity146 phenotype. Screening for the mutation in all suspected @entity4018 cases could be beneficial in differentiating @entity1 with reactive and clonal marrow @entity82 .
