A sensitive sandwich enzyme immunoassay for @entity1 @entity5632 using Fab'-horseradish peroxidase conjugate: methods and results in normal subjects and @entity1 with XXXX .

@entity1 @entity852 @entity66 @entity5632
A sensitive sandwich enzyme immunoassay (EIA) for @entity1 @entity5632 (Mb) was developed. Serum Mb was assayed by incubation with an anti-Mb rabbit IgG-coated @entity852 ball and then with affinity-purified anti-Mb Fab'-horseradish peroxidase (HRP) conjugate. The HRP activity bound to the @entity852 ball was assayed fluorimetrically. The sensitivity was 3.1 pg/tube and serum Mb levels of 0.4-1000 micrograms/l could be determined. The recoveries of Mb added to @entity1 sera at 3 concentrations were 92.8-99.8%. The within- and between-assay coefficients of variations (CV values) were both below 10%. The regression equation of values determined by EIA and radioimmunoassay (RIA) was y(EIA) = 0.964x (RIA)--2.30 and the correlation coefficient was 0.984. The mean normal serum concentration of Mb was 21.5 +/- 6.0 micrograms/l (mean +/- SD) in males and 16.9 +/- 5.8 micrograms/l in females. The significance of serum Mb determination by the EIA in @entity66 was confirmed. The present EIA for Mb is more sensitive and economical than RIA and should be useful for determining Mb in biological materials.
