XXXX gene plays a role during @entity3392 pronephros development.

@entity154 @entity26 @entity600 @entity177 @entity3202 @entity43331 @entity71537 @entity280 @entity3392
@entity280 (pVHL)-mediated ubiquitination of @entity43331 plays a central role in the cellular responses to changes in @entity26 availability. In the present study, using @entity3392 as a model, we showed that specific knockdown of endogenous @entity71537 leads to @entity600 and @entity177 . Knockdown of @entity71537 resulted in abnormal kidney development, including curved and cystic pronephric tubule or/and cystic and @entity3202 . Co-injecting capped @entity71537 messenger RNA (mRNA) partially rescued pronephros morphant phenotype, confirming the specificity of the morpholino oligonucleotide (MO)-induced @entity154 . In keeping with the pronephros phenotype, renal function was affected as well in @entity71537 morphants. Dextran clearance abilities of @entity71537 morphants were significantly reduced as compared with those of control embryos. Further analysis indicated that glomerular integrity is impaired in @entity71537 morphants, while the organization of pronephric duct was minimally affected. @entity71537 morphants display global increased vegf signaling and angiogenesis. In addition, we found that @entity71537 morphants displayed elevated expression of vegfa in podocytes and increased angiogenesis at pronephric glomerulus and the nearby vessels. Treatment of vegf inducer to embryos also caused pronephros phenotype resembling @entity71537 morphants, further supporting that increased vegfa signaling contribute to the pronephros morphant phenotype. Our study establishes the @entity3392 as an alternative vertebrate model system for studying @entity71537 function during kidney development.
