Incidence of phenotypic aberrations in a series of 467 @entity1 with B XXXX : basis for the design of specific four-color stainings to be used for minimal @entity66 investigation.

@entity1 @entity19002 @entity2902 @entity2214 @entity1071 @entity66 @entity706 @entity1075 @entity5 @entity5734 @entity1074 @entity1072 @entity6491 @entity3052 @entity5893 @entity2282 @entity1073 @entity7527 @entity2935 @entity10317
Multiparameter immunophenotypic analysis of neoplastic cells has proven to be of great help for the investigation of minimal @entity66 in @entity3052 ; however, its utility has not been systematically explored in B cell @entity1071 . The aim of the present study was to investigate the incidence of phenotypic aberrations in a series of 467 consecutive @entity1072 through the comparison of the phenotypic characteristics of @entity5 vs normal peripheral blood (n = 10) and bone marrow (n = 10) B cells, in order to explore the applicability of this strategy for minimal @entity66 monitoring. An additional goal of our study was to evaluate the sensitivity of multiparameter flow cytometry for the detection of minimal @entity66 in @entity1072 through dilutional experiments (n = 19). From the @entity1 analyzed 382 corresponded to @entity1072 /small @entity1072 (353 typical and 29 atypical); five to @entity6491 ; 13 to @entity5734 ; 12 to @entity706 ; 14 to @entity1074 ; 22 were @entity706 ; and 19 @entity1073 . The following triple stainings were systematically applied to both normal and leukemic samples: FMC7/ @entity5893 / @entity1075 , @entity10317 / @entity7527 / @entity1075 , @entity19002 / @entity2902 / @entity1075 , @entity2282 / @entity2935 / @entity1075 and sIg/sIg(lambda)/ @entity1075 . Overall, 98% of the @entity1072 cases displayed aberrant phenotypes at diagnosis with no significant differences being found between cases analyzed in peripheral blood vs bone marrow samples. The most common types of aberrant criteria detected included asynchronous antigen expression (92%) and antigen over-expression (54%); abnormally light scatter characteristics were found in 17% of the cases. Most of the cases studied (90%) displayed four or more phenotypic aberrations. Once @entity1 were divided according to the different diagnostic subgroups, the overall incidence of aberrant phenotypes ranged from 79 to 80% among atypical @entity1072 /small @entity1072 and @entity6491 to 97% of @entity706 and 100% of typical @entity1072 /small @entity1072 , @entity5734 , @entity706 , @entity1074 and @entity1073 . Based on the aberrant phenotypes detected unique four-color stainings could be built for the specific identification of aberrant phenotypes. These include @entity10317 / @entity7527 / @entity1075 / @entity5893 and sIg(kappa)/sIg(lambda)/ @entity1075 / @entity5893 for @entity2214 /small @entity1072 and @entity6491 , @entity19002 / @entity2902 or @entity10317 / @entity1075 / @entity2935 for @entity5734 , FMC7/ @entity10317 / @entity1075 / @entity19002 and sIg(kappa)/sIg(lambda)/ @entity10317 / @entity1075 for @entity1074 , @entity10317 / @entity7527 / @entity1075 / @entity2282 for @entity706 and @entity2282 / @entity10317 / @entity1075 / @entity5893 for @entity1073 . Serial dilutional experiments showed that the sensitivity level of immunophenotyping ranges between 10(-4) and 10(-5). In summary, the present study shows that immunophenotypic analysis allows the identification of aberrant phenotypes in 98% of @entity1072 and these phenotypes can be used for minimal @entity66 monitoring with a sensitivity limit of 10(-4)-10(-5).
