Immunosuppressive properties of @entity1138 -incubated XXXX myeloid blood cells (MIC) in vitro.

@entity1 @entity1139 @entity1138
UNASSIGNED: Previous animal studies showed that donor-derived blood cells treated with @entity1138 ( @entity1139 ) prolong allograft survival when injected into recipients. This model was effective with whole blood, peripheral blood mononuclear cells (PBMC) (monocytes being the active cell subpopulation) or dendritic cells. In view of a potential clinical application, we study now the immunosuppressive properties of @entity1 myeloid cells in vitro. Mature dendritic cells (generated from na  ve monocytes) or monocytes treated with @entity1138 do not or only weakly inhibit allogeneic T cells in vitro, whereas cells in an early differentiation state between monocytes and DC exert suppressive activity when treated with @entity1139 . In contrast, DC generated from @entity1139 -treated monocytes show the morphology and phenotype of early immature DC (iDC) and suppress T-cell responses. It is known that untreated monocytes injected into a recipient encounter a cytokine milieu which differentiates them to stimulatory DC. In our in vitro experiment @entity1139 -treated monocytes cultured in a DC-maturing milieu transform themselves into suppressive early iDC. This reproduces a process which takes place when administering @entity1139 -monocytes to a recipient. In conclusion, @entity1 @entity1139 -DC or @entity1139 -monocytes are not or only weakly suppressive in vitro. When @entity1139 -monocytes are differentiated to DC the resulting cells become suppressive.
