Detection of @entity519 in XXXX brain tumor xenografts using a modified comet assay.

@entity1 @entity5 @entity19 @entity519 @entity28087 @entity35
We used the standard comet assay successfully to generate in vitro dose-response curves under oxic and @entity519 conditions. We then made mixtures of cells that had been irradiated with 3 and 9 Gy of X-rays to simulate two subpopulations in a @entity5 , but efforts to accurately detect and quantify the subpopulations using the standard comet assay were unsuccessful. Therefore, we investigated a modified comet assay to determine whether it could be used for measuring @entity519 in our model systems. @entity1 cells were grown as @entity5 in athymic @entity19 ; @entity1 and U87 MG cells were grown as intracerebral (i.c.) @entity5 in athymic @entity35 . Animals were injected with @entity28087 , irradiated, and euthanized. @entity5 and normal brains were removed, and the cells were analyzed using a modified comet assay. Differences in comet tail moment distributions between @entity5 and contralateral normal brain, using tail moments at either the 25th or 50th percentile in each distribution, were taken as measures of the degree of @entity5 . For U251 @entity5 , there was a positive relationship between @entity5 size and the degree of @entity519 , whereas preliminary data from U87 MG i.c. @entity5 showed less @entity519 and no apparent relationship between @entity5 size and @entity519 .
