Hydrogen Suppresses XXXX /Reoxygenation-Induced Cell Death in Hippocampal Neurons Through Reducing Oxidative Stress.

@entity2308 @entity1976 @entity2310 @entity519 @entity1167 @entity35 @entity438
BACKGROUND _ AIMS: @entity1167 ( @entity1167 ) is a cerebral protection technique that has been used in the operations involving the aortic arch and @entity438 for decades. We previous showed that @entity1167 treated @entity35 developed a significant oxidative stress and apoptosis in neurons. We here intend to investigate the protective the effect of hydrogen against oxidative stress-induced cell injury and the involved mechanisms using an in vitro experimental model of @entity519 /reoxygenation (H/R) on HT-22 cells. METHODS: The model of H/R was established using an airtight culture container and the anaeropack. Measurement of mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) production was used H2DCFDA and JC-1 staining. Western blot was used for the quantification of @entity2308 , p- @entity2308 , @entity1976 , @entity2310 and cleaved caspase-3 proteins. The microRNA (miRNA) profile in hippocampal neurons from @entity35 model of @entity1167 was determined by miRNA deep sequencing. RESULTS: The elevation of ROS and reduction of MMP were significantly induced by the treatment with @entity519 for 18 h followed by reoxygenation for 6 h. Hydrogen treatment significantly reduced H/R-caused cell death. The levels of p- @entity2308 (Ser 473) and @entity1976 were significantly increased while @entity2310 and cleaved caspase-3 were decreased by hydrogen treatment on the model of H/R. The expression of miR-200 family was significantly elevated in model of @entity1167 and H/R. Hydrogen administration inhibited the H/R-induced expression of miR-200 family in HT-22 cells. In addition, inhibition of miR-200 family suppressed H/R-caused cell death through reducing ROS production. CONCLUSIONS: These results suggest that H/R causes oxidative stress-induced cell death and that the hydrogen protects against H/R-induced cell death in HT22 cells, in part, due to reducing expression of miR-200 family.    2015 S. Karger AG, Basel.
