High @entity413 induces the release of XXXX through the inhibition of @entity3957 production in HUVECs.

@entity3958 @entity1 @entity455 @entity6 @entity63 @entity3959 @entity2232 @entity8835 @entity3957 @entity413
UNASSIGNED: @entity3957 ( @entity3958 ) has recently been identified as an endogenous gaseous signaling molecule. In the vascular system, the formation of @entity3958 is catalyzed by @entity3959  y  -lyase (CSE). Previous studies have demonstrated the protective effects of @entity3958 on @entity63 in various types of tissue. However,, little is known about the role of @entity3958 in @entity6 -associated @entity455 . Thus, the aim of the present study was to examine the possible role of @entity3958 in high @entity413 -induced @entity455 , and to explore the underlying mechanisms. @entity1 umbilical vein endothelial cells (HUVECs) were isolated from @entity1 umbilical veins. The levels of @entity3958 following treatment with various levels of @entity413 were determined and the secretion of @entity2232 ( @entity2232 ) was measured by ELISA. The mRNA and protein expression of CSE in the HUVECs was determined by real-time RT-PCR and western blot analysis, respectively. Treatment with high @entity413 (25  mmol/l) for 48  h significantly increased the secretion of @entity2232 by HUVECs, with the concomitant suppression of @entity3958 production and CSE protein expression. The increase in exogenous @entity3958 levels through the administration of @entity8835 ( @entity8835 ) attenuated the high @entity413 -induced downregulation of CSE protein expression, and significantly inhibited the secretion of @entity2232 . These results suggest that the downregulation of CSE protein expression and the subsequent decrease in @entity3958 production play a role in high @entity413 -induced @entity455 possibly by increasing the secretion of @entity2232 by endothelial cells.
