Comparative proteome analysis of alkaliphilic Bacillus sp. N16-5 grown on different XXXX sources.

@entity6122 @entity3363 @entity535 @entity4583 @entity1735 @entity27753 @entity3913 @entity3649 @entity505 @entity413
To determine the impact of @entity3363 on the metabolic pathway in alkaliphiles, proteomes were obtained from cultures containing different @entity3363 and were resolved on two-dimensional gel electrophoresis (2-DE). The proteomes were compared to determine differentially expressed proteins. A novel alkaliphilic bacterium (alkaliphilic Bacillus sp. N16-5 isolated from Wudunur Soda Lake, China) was isolated in media with five different @entity505 sources ( @entity413 , @entity3913 , @entity3649 , @entity4583 , and @entity6122 ). Comparative proteome analysis identified 61 differentially expressed proteins, which were mainly involved in @entity1735 metabolism, @entity535 transport, and metabolism, as well as energy production and conversion. The comparison was based on the draft genome sequence of strain N16-5. The abundance of enzymes involved in central metabolism was significantly changed when exposed to various @entity3363 . Notably, catabolite control protein A (CcpA) was up-regulated under all @entity505 sources compared with @entity413 . In addition, @entity27753 exhibited a stronger effect than hexose in CcpA-mediated @entity505 catabolite repression. These results provided a fundamental understanding of @entity1735 metabolism in alkaliphiles.
