[The incidence of the pituitary autoantibodies in XXXX ].

@entity1 @entity2743 @entity2975 @entity5010 @entity792
INTRODUCTION: It is known that in the sera of @entity1 with Graves, @entity792 we can detect autoantibodies against pituitary antigens. The aim of the study was evaluation of pituitary autoantibodies in @entity2743 @entity1 using immunoblotting methods. MATERIAL AND METHODS: Studies were performed in 32 @entity2743 @entity1 , 25 @entity1 (age range: 31-67 yrs, median: 49.9 +/- 9.4) and 7 @entity1 (age range: 41-58 yrs, median: 51.0 +/- 7.1). All @entity1 presented signs and symptoms typical of @entity2743 . The diagnosis was confirmed by laboratory tests (TSH, fT(3), fT(4), @entity5010 antibodies). Sera of control subjects were obtained from 10 healthy blood donors, 7 @entity1 , 3 @entity1 (age range 21-45 yrs, median: 30.6 +/- 7.1). Incidence of pituitary autoantibodies was assessed by @entity2975 electrophoresis gel and western-blotting. Pituitary microsomes were obtained from @entity1 pituitary tissues by ultracentrifugation and solubilisation in 1% desoxycholic acid. RESULTS: In 23 sera from 32 we detected autoantibodies against pituitary microsomal antigens. 16 sera were reacting with 55 kDa antigen, 10 sera with 67 kDa, 6 sera with 60 kDa, 5 sera with 52 kDa and 4 sera with 105 kDa. It is important to note that 6 sera were reacting with 57 and 55 kDa, and 5 sera with 55, 60 and 67 kDa. CONCLUSIONS: In sera of @entity2743 @entity1 autoantibodies against pituitary microsomal antigens can be frequently detected. The most frequent are antibodies against 55, 60 and 67 kDa antigens.
