Techniques for detection of XXXX .

@entity1 @entity1778 @entity41
Analysis of @entity1778 and @entity1778 -associated markers following standard or high-dose treatments is crucial in order to evaluate the efficacy of therapeutic strategies. During the last decade, several techniques have been proposed and used for detecting @entity41 ( @entity41 ). Each approach is characterized by advantages and limitations, mainly related to its sensitivity and specificity. The general limitations of such tests originates from the size of the sample which can be analysed and the heterogeneous distribution of @entity1778 after treatment. Clinically useful methods for detecting @entity1778 require not only sensitivity but also speed and reproducibility. The rate of false negative tests is low with polymerase chain reaction as well as flow cytometric analysis. Usually, @entity1 without persistent cells carrying @entity1778 -associated markers have a lower risk of relapse. However, the detection of a persistent marker at one time point in complete remission cannot be considered a reliable indicator of @entity41 , whereas increase of positive signals or reappearance of leukemic markers usually precedes relapse. It is likely that one single approach will not allow the monitoring of the majority of @entity1 and that a combination of techniques will be needed. Definitive results will be obtained only through prospective studies in @entity1 receiving standardised therapy. Studies in which therapeutic strategies are designed according to the results provided by techniques for detecting @entity41 will be necessary to assess the relevance of their contribution to the treatment of @entity1778 .
