Gene expression profile of @entity22321 transporters and cytotoxic effect of XXXX and @entity1443 in an @entity43 cell line in vitro.

@entity43 @entity1 @entity22149 @entity5018 @entity36620 @entity21552 @entity21123 @entity43423 @entity22321 @entity1443 @entity57640
PURPOSES: To determine the basic expression of @entity22321 transporters in an @entity43 cell line, and to investigate whether low concentrations of @entity1443 and @entity5018 inhibited the growth of this cell line in vitro. METHODS: @entity1 cells were exposed to different concentrations of @entity1443 (1.5 to 15  g/mL) and @entity5018 (2.0 to 20  g/mL) for 24 to 48 hours. The cellular growth was assessed using a cell viability assay. Cellular morphology was determined by fluorescence microscopy. The gene expression profile of @entity22321 transporters was determined by assessing a panel including 42 genes of the @entity22321 transporter superfamily. RESULTS: We observed a significant decrease in @entity1 cell growth after exposure to 15  g/mL of @entity1443 for 24 (p=0.02) and 48 hours (p=0.01), or to 20  g/mL of @entity5018 for 48 hours (p=0.04). Assessing the morphology of @entity1 cells did not reveal evidence of extensive apoptosis. @entity1 cells had a reduced expression of the genes @entity21123 , @entity22149 , @entity36620 , @entity21552 , @entity57640 and @entity43423 within the @entity22321 transporter superfamily. CONCLUSIONS: This study provides in vitro evidence of inhibitory effects of growth in therapeutic concentrations of @entity1443 and @entity5018 on @entity1 cells. Additionally, @entity1 cells presented a reduced expression of the @entity21123 , @entity22149 , @entity36620 , @entity21552 , @entity57640 and @entity43423 transporters.
