A microRNA survival signature (MiSS) for advanced XXXX .

@entity43 @entity1 @entity5 @entity62965 @entity72540 @entity72541
OBJECTIVES: MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate gene expression primarily through post-transcriptional modification. We tested the hypothesis that miRNA expression is associated with overall survival in advanced @entity43 . METHODS: Cases included newly diagnosed @entity1 with @entity43 . RNA from a training set of 62 cases was hybridized to an miRNA microarray containing 470 mature @entity1 transcripts. Cox Regression was performed to identify miRNAs associated with overall survival. External validation was performed using quantitative RT-PCR miRNA assays in an independent test set of 123 samples. MiRNA targets and associated biologic pathways were predicted in silico. RESULTS: Of all @entity1 , 80% had high-grade, stage IIIC @entity5 and 64% underwent optimal cytoreduction. The median survival for the entire cohort was 49  4 months. The training set identified 3 miRNAs associated with survival-- @entity72540 , @entity62965 , and @entity72541 . An miRNA signature containing @entity62965 and @entity72541 was most strongly associated with overall survival in the training set (HR=2.96, 95% CI: 1.51-5.78). This miRNA survival signature (MiSS) was validated in the test set (HR=1.71, 95% CI: 1.05-2.78). The MiSS was independent of FIGO stage and surgical debulking. CONCLUSIONS: The data suggest that an MiSS that contains @entity62965 and @entity72541 is negatively associated with overall survival in advanced serous @entity43 . This signature, when further validated, may be useful in individualizing care for the @entity43 @entity1 . Pathway analyses identify biologically plausible mechanisms.
