Sonoporation enhances chemotherapeutic efficacy in XXXX cells in vitro.

@entity632 @entity4653 @entity5 @entity995
PURPOSE: To study the ability of ultrasound (US) and microbubbles (MB) to enhance chemotherapeutic efficacy against @entity995 Y79 cells in vitro. METHODS: The experiment was performed in three stages. The authors first compared cell viability of Y79 cells exposed to @entity632 versus cells exposed to @entity632 combined with low-intensity, low-frequency US + MB. They then evaluated enhanced cell permeability by studying the intensity of intracellular fluorescence in cells exposed to @entity632 versus those exposed to @entity632 with US + MB. Lastly they evaluated the morphologic characteristics of the cells by @entity4653 ( @entity4653 ) to identify the presence of pores. RESULTS: The Y79 cells exposed to @entity632 with US + MB showed a significant decrease in cell viability at 72 hours compared with those exposed to @entity632 alone (P = 0.02). Cells also showed immediate increased permeability to @entity632 with the addition of US + MB compared with @entity632 alone, which continued to increase over 60 minutes. @entity4653 did not demonstrate physical pores at the lowest US + MB intensity shown to enhance intracellular @entity632 fluorescence. CONCLUSIONS: US + MB facilitates the uptake of chemotherapy in @entity995 Y79 cells in vitro. This occurs in the absence of visible pores, suggesting a possible secondary mechanism for increased drug delivery. This experiment is the first step toward enhancing chemotherapy with sonoporation in the treatment of intraocular @entity5 . This technique may lead to more effective chemotherapy treatments with less collateral damage to ocular tissues and may allow reduced systemic dosage and systemic side effects.
