Immunohistological study of mononuclear cell infiltrate in XXXX .

@entity39 @entity5 @entity11 @entity816 @entity463
Sixty-five @entity11 ( @entity816 grade 3 and 4 and @entity463 ) were examined by means of immunoperoxidase staining on frozen tissue using various monoclonal antibodies directed against macrophages, lymphocytes and natural killer cells. Depending on the antibody used, the presence of macrophages in @entity5 ranged from 85%-100%. Many of the @entity5 contained substantial numbers of macrophages not only, as expected, in @entity39 areas but also in intact @entity5 tissue. Eighty-nine percent of 39 @entity5 tested contained Fc receptor-bearing mononuclear cells in viable @entity5 . In 100% of 44 @entity5 tested for HLADR class 2 major histocompatibility complex antigen this antigen was detected in the macrophages. In 40% of these 44 cases, HLADR antigen was also present on the @entity5 cells. Eighty-eight percent of 53 @entity5 tested contained T cells in viable @entity5 and the majority of these cells were T cytotoxic/suppressor (T8). Twenty-four percent of 33 @entity5 contained no T helper/inducer (T4) lymphocytes and in the other 76% there were few positive cells. Only 9% of 21 @entity5 contained natural killer cells (NK). B cells were absent from 88% of 61 @entity5 and almost all of the remainder contained only a small number of B cells. The findings are discussed with reference to a possible host immune response to @entity11 and relevant literature is reviewed.
