The XXXX @entity2124 gene locus: structure, evolution, and allelic variations.

@entity1 @entity9091 @entity535 @entity5999 @entity377 @entity2124 @entity5804 @entity10718 @entity7510 @entity10716
Genomic clones containing the closely related genes for @entity1 @entity2124 (hGH) and chorionic somatomammotropin ( @entity10716 ) were obtained from genomic @entity5999 and cosmid libraries. The entire GH/ @entity10716 chromosomal locus was reconstructed utilizing overlapping restriction fragments characterized from the isolated clones. The hGH/ @entity10716 locus contains two GH genes and three @entity10716 genes spanning 48 kb of DNA in the order: 5'-( @entity2124 / @entity10718 / @entity7510 / @entity9091 / @entity5804 )-3', confirming analysis of cosmid clones obtained from a different @entity1 library (Barsh et al., 1983). To complete the characterization of the @entity10716 genes, the @entity377 sequence of the @entity10718 gene was determined. Sequence analysis revealed a mutation of the 5' splice site at the exon II-intron B boundary, suggesting that the @entity10718 gene is a pseudogene. The @entity377 sequence of an allelic variant of the @entity5804 gene was determined and found to contain a single @entity535 substitution and the deletion of a single codon. The hGH/ @entity10716 gene locus was further characterized by the localization of at least 27 Alu-type repetitive sequences and identification of three unique sequences in the vicinity of several hGH and @entity10716 genes which define the probable breakpoints of the evolutionary duplication units. These data, combined with the @entity377 sequences of all five GH and @entity10716 genes, indicate that the hGH/ @entity10716 gene locus has evolved by duplication mechanisms. Evidence for the occurrence of at least one gene conversion event involving the @entity7510 gene precursor and the @entity5804 gene was found, indicating that the hGH/ @entity10716 gene locus has evolved by concerted mechanisms. The structure of the @entity10716 genes is discussed in light of recent studies of @entity10716 genes from other @entity1 species.
