[Application of BIOMED- 2 standardized Ig gene rearrangement system in XXXX ].

@entity1582 @entity7513 @entity1016
OBJECTIVE: To explore the application of BIOMED- 2 standardized immunoglobulin    Ig   gene rearrangement system in the diagnosis of @entity1582   MM   , and the significance of clonality analysis by multiplex-PCR amplifications. METHODS: A total of 167 cases of MM bone marrow samples from 2009 to 2013, and 20 cases of reactive plasmacytosis used as the controls were included in this study. Multiplex-PCR amplifications were performed and the Ig gene rearrangements were analyzed using BIOMED-2 standardized clonality analysis system. RESULTS:  -  Of 167 MM cases, 107 showed @entity7513 VH-JH rearrangement, 33 showed @entity7513 DH-JH rearrangement, and 30% showed @entity7513 DH-JH rearrangement in 60 @entity7513 VH-JH rearrangement negative MM cases. The difference was statistically significant between @entity7513 VH-JH rearrangement positive and negative cases   14.0% vs 30.0%, P=0.032   . The total positive rate of @entity7513 VH-JH, @entity7513 DH-JH and @entity1016 was 94.6%. The 20 reactive plasmacytosis   RP   cases showed negative Ig gene rearrangement.  - Of 167 MM cases, 9   5.4%   showed clonal @entity7513 rearrangement by agarose electrophoresis were confirmed as polyclonality by capillary electrophoresis.  -  Of 53 MM cases who have been detected by Ig gene rearrangement system and fluorescence in situ hybridization   FISH   for @entity7513 simultaneously, 36 showed @entity7513 rearrangement, 26 showed FISH @entity7513 positive, and the difference was statistically significant    67.9% vs 49.1%, P=0.049   . CONCLUSION: Combined detection of @entity7513 VH- JH, @entity7513 DH- JH and @entity1016 could improve the positive rate of MM clonality dramatically, and measurement of @entity7513 DH-JH rearrangement was more important in the @entity7513 VH- JH negative cases. Ig gene rearrangement system was a faster and more sensitive method than FISH @entity7513 . Application of BIOMED- 2 standardized immunoglobulin    Ig    gene rearrangement system is of significance for MM diagnosis.
