Role of monocyte chemotactic protein-1/CC chemokine ligand 2 on gamma delta T lymphocyte trafficking during @entity32 induced by lipopolysaccharide or XXXX bacille Calmette-Gu  rin.

@entity1143 @entity32 @entity558 @entity1750 @entity19 @entity13766 @entity619 @entity1990 @entity2497 @entity6621
Gammadelta T lymphocytes are involved in a great variety of inflammatory and infectious responses. However, the mechanisms by which gammadelta T lymphocytes migrate to inflamed sites are poorly understood. In this study we investigate the role of monocyte chemotactic protein (MCP)-1 in regulating gammadelta T cell migration after @entity558 or @entity2497 bacille Calmette-Gu  rin (BCG) challenge. @entity558 -induced gammadelta T cell influx was significantly inhibited by either pretreatment with @entity619 or @entity6621 Lister 35-kDa chemokine binding protein, vCKBP, a CC chemokine neutralizing protein, suggesting a role for CC chemokines in this phenomenon. @entity558 stimulation increased the expression of @entity1750 mRNA and protein at the @entity32 site within 6 h. It is noteworthy that @entity558 was unable to increase @entity1750 production or gammadelta T cell recruitment in C3H/HeJ, indicative of the involvement of @entity1990 . Gammadelta T cells express @entity13766 @entity13766 . Pretreatment with anti- @entity1750 mAb drastically inhibited @entity558 -induced in vivo gammadelta T cell mobilization. Indeed, @entity1750 knockout @entity19 were unable to recruit gammadelta T cells to the @entity1143 after @entity558 stimulation, effect that could be restored by coadministration of @entity1750 . In addition, BCG-induced gammadelta lymphocyte accumulation was significantly reduced in @entity1750 knockout @entity19 when compared with wild-type @entity19 . In conclusion, our results indicate that @entity558 -induced gammadelta T lymphocyte migration is dependent on @entity1990 and sensitive to both @entity619 and CC chemokine-binding protein inhibition. Moreover, by using @entity1750 neutralizing Abs and genetically deficient @entity19 we show that @entity558 - and BCG-induced gammadelta T lymphocyte influx to the @entity1143 of @entity19 is mainly orchestrated by the CC chemokine @entity1750 .
