Angelica polymorpha Maxim Induces Apoptosis of Human SH-SY5Y XXXX Cells by Regulating an Intrinsic Caspase Pathway.

@entity137 @entity627 @entity589 @entity588 @entity522 @entity5 @entity2423 @entity584 @entity8186 @entity490 @entity521 @entity14939 @entity9459 @entity2275
UNASSIGNED: Angelica polymorpha Maxim root extract (APRE) is a popular herbal medicine used for treating stomachache, @entity490 , @entity2275 , and @entity627 ; however the effect of APRE on @entity5 cells has not yet been explored. Here, we examined @entity137 seen on target @entity521 cells (NB) using cell viability assays, @entity14939 visualization of fragmented DNA, and Western blotting analysis of candidate signaling pathways involved in proliferation and apoptosis. We demonstrated that APRE reduced cell viability in NB to a greater extent than in fibroblast cells. In addition, we found that APRE could inhibit the three classes of MAPK proteins and could also down-regulate the PI3K/ @entity584 / @entity9459 activity all being relevant for proliferation and survival. APRE could also up-regulate @entity589 expression and down-regulate @entity588 and @entity2423 . With APRE treatment, depolarization of mitochondria membrane potential and activation of @entity522 was demonstrated in the SH-SY5Y cells. We could not found increased activity of death receptor and @entity8186 as markers of the extrinsic apoptosis pathway for the APRE treated cells. In presence of a @entity522 siRNA and a pan-caspase inhibitor, APRE could not reduce the viability of NB cells to a significant degree. So we predicted that with APRE, the intrinsic pathway was solely responsible for inducing apoptosis as we also showed that the non-caspase autophagy pathway or ER stress-ROS mediated pathways were not involved. These findings demonstrate that an intrinsic mitochondria-mediated apoptosis pathway mediates the apoptotic effects of APRE on SHSY5Y cells, and that APRE shows promise as a novel agent for @entity521 therapy.
