Associations between genetic variation in one- XXXX metabolism and LINE-1 DNA methylation in histologically normal breast tissues.

@entity1 @entity1501 @entity0 @entity9769 @entity5 @entity7612 @entity71386 @entity21612 @entity45494 @entity505 @entity4334 @entity105 @entity285
UNASSIGNED: Genome-wide DNA hypomethylation is an early event in the carcinogenic process. Percent methylation of long interspersed @entity4334 element-1 (LINE-1) is a biomarker of genome-wide methylation and is a potential biomarker for @entity0 . Understanding factors associated with percent LINE-1 DNA methylation in histologically normal tissues could provide insight into early stages of @entity105 . In a cross-sectional study of 121 healthy @entity1 with no prior history of @entity5 who underwent reduction mammoplasty, we examined associations between plasma and breast @entity1501 , genetic variation in one- @entity505 metabolism, and percent LINE-1 methylation using multivariable regression models (adjusting for race, oral contraceptive use, and @entity285 use). Results are expressed as the ratio of LINE-1 methylation relative to that of the referent group, with the corresponding 95% confidence intervals (CI). We found no significant associations between plasma or breast @entity1501 and percent LINE-1 methylation. Variation in @entity9769 , MTR, and @entity7612 were significantly associated with percent LINE-1 methylation. Variant allele carriers of @entity9769 @entity71386 had 4% lower LINE-1 methylation (Ratio 0.96, 95% CI 0.93-0.98), while variant allele carriers of MTR @entity45494 (Ratio 1.03, 95% CI 1.01-1.06) and @entity7612 @entity21612 (Ratio 1.03, 95% CI 1.01-1.06) had 3% higher LINE-1 methylation, compared to those carrying the more common genotypes of these SNPs. DNA methylation of LINE-1 elements in histologically normal breast tissues is influenced by polymorphisms in genes in the one- @entity505 metabolism pathway. Future studies are needed to investigate the sociodemographic, environmental and additional genetic determinants of DNA methylation in breast tissues and the impact on @entity0 susceptibility.
