The role of interstitial Cajal-like cells in the formation of cholesterol stones in XXXX gallbladder.

@entity17545 @entity840 @entity1319 @entity5990
OBJECTIVES: To investigate the effect of interstitial Cajal-like cells (ICLCs) on contraction of gallbladder muscle strips; and to analyze the changes of ICLCs during cholesterol @entity5990 formation in @entity840 . METHODS: The cholesterol @entity5990 animal model was made by feeding @entity840 with @entity17545 ( @entity17545 ). In vitro isolated gallbladder muscle strips were prepared. Gallbladder motility was assessed by the contraction frequency and amplitude of slow wave in response to CCK-8. The alteration in ICLC density was estimated by using immunohistochemistry. The expression of c-kit and stem cell factor (SCF) were determined. RESULTS AND CONCLUSIONS: The amplitude and frequency of slow wave was significantly lower in gallbladder muscle strips with the impaired ICLCs. And it is correlated with the decreased contractile response to CCK-8. In @entity17545 @entity840 , the ICLC density and bile flow in response to CCK-8 were remarkably decreased. The results indicated that gallbladder ICLCs can create slow wave potential, and also get involved in the regulation of CCK-8 induced gallbladder smooth muscle motility. In the process of cholesterol @entity5990 formation, ICLC density clearly decreased. This further @entity1319 . The decrease in ICLC density may result from decreased expression of c-kit and SCF during cholesterol @entity5990 formation.
