Skeletal @entity121 is associated with derangements in mitochondrial bioenergetics (but not XXXX ) in a rodent model of @entity295 .

@entity295 @entity19 @entity26 @entity1295 @entity177 @entity855 @entity4308 @entity121 @entity65 @entity2332 @entity2383
@entity121 is a common feature of severe @entity295 and @entity177 . Recent evidence implicates bioenergetic dysfunction and oxidative damage as important underlying pathophysiological mechanisms. Increased abundance of @entity4308 ( @entity4308 ) in @entity295 suggests @entity2383 , which may reduce mitochondrial coupling efficiency but limit reactive @entity26 species (ROS) production. Using a @entity19 model, we examined metabolic, cardiovascular, and skeletal muscle contractile changes following induction of peritoneal @entity295 in wild-type and @entity4308 (-/-) @entity19 . Mitochondrial membrane potential (    m) was measured using two-photon microscopy in living diaphragm, and contractile function was measured in diaphragm muscle strips. The kinetic relationship between membrane potential and @entity26 consumption was determined using a modular kinetic approach in isolated mitochondria. @entity295 was associated with significant whole body metabolic suppression, @entity2332 , and @entity65 . Maximal force generation was reduced and @entity1295 accelerated in ex vivo diaphragm muscle strips from septic @entity19 .     m was lower in the isolated diaphragm from septic @entity19 despite normal substrate oxidation kinetics and @entity121 . Even though wild-type @entity19 exhibited an absolute 26    6% higher @entity4308 protein abundance at 24 h, no differences were seen in whole animal or diaphragm physiology, nor in survival rates, between wild-type and @entity4308 (-/-) @entity19 . In conclusion, this @entity19 @entity295 model shows a hypometabolic phenotype with evidence of significant @entity65 . This was associated with lower     m and alterations in mitochondrial @entity855 turnover and the phosphorylation pathway. However, @entity4308 does not play an important functional role, despite its upregulation.
