A clinical instrument for combined raman spectroscopy-optical coherence tomography of XXXX .

@entity1 @entity227 @entity5 @entity1489 @entity272 @entity26673
BACKGROUND AND OBJECTIVE: The current standard for diagnosis of @entity1489 is @entity227 followed by biopsy and histopathology. This process can be invasive, subjective, time consuming, and costly. Optical techniques, including Optical Coherence Tomography (OCT) and Raman Spectroscopy (RS), have been developed to perform non-invasive characterization of @entity272 based on either morphological or biochemical features of disease. The objective of this work is to report a clinical instrument capable of both morphological and biochemical characterization of @entity1489 with RS-OCT. MATERIALS AND METHODS: The portable instrument utilizes independent 785   nm RS and 1,310   nm OCT system backbones. The two modalities are integrated in @entity26673   (L) clinical probe. The probe enables sequential acquisition of co-registered OCT and RS data sets. The axial response of the RS collection in the skin was estimated using scattering phantoms. In addition, RS-OCT data from @entity1 with @entity5 are reported. RESULTS: The RS-OCT instrument is capable of screening areas as large as 15   mm (transverse) by 2.4   mm (in depth) at up to 8   frames/second with OCT, and identifying locations to perform RS. RS signal is collected from a 44     m transverse spot through a depth of approximately 530     m. RS-OCT data sets from a superficial scar and a nodular BCC are reported to demonstrate the clinical potential of the instrument. CONCLUSION: The RS-OCT instrument reported here is capable of morphological and biochemical characterization of cancerous @entity272 in a clinical setting. OCT can visualize microstructural irregularities and perform an initial morphological analysis of the lesion. The images can be used to guide acquisition of biochemically specific Raman spectra. The two data sets can then be evaluated with respect to one another to take advantage of the mutually complimentary nature of RS and OCT.
