Immunochemical cross-reactivity of XXXX phospholipase A2 enzymes from @entity32766 ( @entity32766 ) venom using polyclonal antibodies.

@entity137 @entity4 @entity1701 @entity32766 @entity10575 @entity2359
Rabbit antibodies were prepared against purified phospholipase A2 ( @entity10575 ) from @entity32766 ( @entity32766 ) venom. The PLA2 has haemolytic, @entity2359 , myotoxic, cytotoxic and @entity4 -inducing activities apart from the catalytic activity. The immunological cross-reactivity of structurally similar @entity2359 PLA2s was investigated using enzyme-linked immunosorbent assay (ELISA) and immunodiffusion. @entity1701 IgG cross-reacted with other purified @entity2359 PLA2s from the same venom. Immunochemical cross-reactions of anti- @entity10575 IgG with @entity10575 , NN-XIb-PLA2, NN-XIII-PLA2, NN-IVb1-PLA2 and NN-Vb-PLA2 were shown by a very high ELISA titre and a single precipitin band on double immunodiffusion agarose plates. The catalytic activity of these PLA2s was inhibited dose-dependently by anti- @entity10575 IgG but was unable to neutralize lethality and @entity2359 in experimental animals injected with @entity2359 PLA2. @entity1701 IgG fails to neutralize myotoxicity and @entity4 -inducing activities of @entity10575 and NN-XIII-PLA2. @entity1701 IgG inhibited cytotoxic effects of @entity10575 dose-dependently, but failed to inhibit NN-XIII-PLA2-induced @entity137 . Direct haemolytic activity of @entity10575 and NN-XIII-PLA2 was inhibited dose-dependently by these antibodies. The results indicate the presence of separate catalytic and pharmacologic site(s).
