Experimental study of @entity1014 kinase gene therapy of XXXX in vitro and in vivo.

@entity1 @entity19 @entity5 @entity14286 @entity74 @entity1014 @entity521 @entity4540
@entity521 arises as a direct result of @entity74 ; therefore, it should be well treated and conquered by gene therapy in future. In this study, @entity521 cell line SH-SY5Y experiments, in vitro and in @entity19 in vivo, were subjected to research @entity1014 kinase suicide gene to treat @entity521 . The plasmid LXpsp-hytk and a plasmid LXSH were transduced separately by lipofectin into @entity1 @entity521 cell line SH-SY5Y. SH-SY5Y-hy and SH-SY5Y-hytk were selected by @entity14286 . Different @entity4540 (GCV) concentration was given to SH-SY5Y-hytk to determine optimal GCV concentration. The cytotoxic effect of GCV on SH-SY5Y-hytk, SH-SY5Y-hy, and SH-SY5Y cells was determined. Scapular subcutaneous @entity5 were established in @entity19 by inoculating 2.5 x 10(6) SH-SY5Y-hytk on their left sides and 2.5 x 10(6) SH-SY5Y-hy cells on their right sides for every @entity19 of treatment group and control group, respectively. After 1 week, mass grew in both sides of all the @entity19 , and from the eighth day on, every @entity19 in treatment group received daily intraperitoneal injection of GCV 50 mg/kg body weight for 14 days; every @entity19 in control group received daily intraperitoneal injection of 1 ml saline for 14 days. On day 22 @entity5 were excised and weighed on the left and right sides, respectively, and apoptosis was detected by TUNEL method. Apoptotic index was calculated on the left and on the right sides, respectively, for every @entity19 in treatment group and control group. The lowest concentration of @entity14286 was 60 microg/ml. The cytotoxic effect of GCV on SH-SY5Y-hytk cells was obvious (IC(50)=0.03 microM), whereas GCV showed almost no cytotoxic effect on SH-SY5Y and SH-SY5Y-hy cells (IC(50)>400 microM). SH-SY5Y-hytk was killed by concentrations of 30 microM GCV effectively and it obviously showed the bystander effect, when SH-SY5Y-hytk remained at least 18% in the mixture culture cells. The @entity5 on the left side was much smaller than that of the right side in control group (p<0.05), and apoptotic index of the left was higher than that of the right in control group (p<0.01). SH-SY5Y-hytk has the bystander effect over 18% SH-SY5Y-hytk of the mixture culture cells at the concentration of 30 microM GCV. The HSV-tk/GCV system was effective in treating SH-SY5Y @entity521 cell line in vivo as well. Our findings suggest that @entity1014 kinase gene therapy could be a potential method for treating @entity521 in the future.
