Different antigens trigger different @entity1198 /Th2 reactions in neonatal mononuclear cells (MNCs) relating to XXXX / @entity9388 expression.

@entity1 @entity7794 @entity7130 @entity9388 @entity1198 @entity10071 @entity379 @entity3646
Neonates are known to have poor cellular immunity, especially poor @entity1198 response. We investigated how neonatal mononuclear cells raised different @entity1198 /Th2 reactions in response to different antigens. Employing @entity7130 (Der p) extract and varicella zoster virus (VZV) as antigens, we assessed @entity1198 /Th2 reactions as demonstrated by @entity3646 / @entity379 production and mRNA expression, and transcriptional factors @entity10071 / @entity9388 mRNA expression in mononuclear cells from @entity1 umbilical cord blood (CBMC). Results showed that @entity7794 induced a dramatic increase of @entity379 production by adult peripheral blood mononuclear cells (PBMC), whereas @entity7794 did not drive CBMC to release significant @entity379 production (1614.7+/-362.0 vs. 49.0+/-29.3,p<0.005). However, Der p induced higher @entity379 production by CBMC than @entity7794 (298.1+/-171.8 vs. 49.0+/-29.3, P=0.047). In contrast, @entity7794 did not induce significant @entity3646 production either by CBMC or by PBMC. Der p induced a comparative @entity3646 production by CBMC and PBMC (2.58+/-0.84 vs. 2.04+/-0.37, p>0.05). A real-time RT-PCR analysis of @entity3646 and @entity379 mRNA expression showed that @entity7794 induced a significantly higher @entity379 , but not @entity3646 , mRNA expression in PBMC than CBMC. Der p did not induce significant difference of @entity379 or @entity3646 mRNA expression in PBMC and CBMC. @entity7794 enhanced @entity1198 -related transcription factor @entity10071 mRNA expression, in association with later down-regulation of Th2-related @entity9388 mRNA expression in PBMC. However, @entity7794 did not up-regulate @entity10071 or down-regulate @entity9388 expression significantly in CBMC. In contrast, Der p induced an early @entity9388 expression and later @entity10071 expression in CBMC. These results suggest that different antigens trigger various @entity1198 /Th2 reactions in PBMC and CBMC resulting from kinetic changes of @entity10071 / @entity9388 expression.
