Induction of PG G/H synthase-2 in @entity291 myometrial cells by interferon-tau requires the activation of the XXXX MAPK pathway.

@entity1441 @entity32589 @entity39979 @entity2924 @entity291 @entity28071 @entity2925
PGs are regulators of a plethora of uterine functions during reproductive processes, including uterine contractility. In @entity291 uterus, the rate-limiting step in PG synthesis is catalyzed by the PG endoperoxide G/H synthase (PGHS) enzymes. It has previously been established that @entity39979 isoform expression is affected by the ruminant-specific interferon (IFN)-tau in @entity291 endometrial cells. Here, we show that @entity39979 mRNA and protein levels are induced by @entity32589 in primary cell cultures from @entity291 myometrium. Treatment with recombinant @entity291 @entity32589 induces the activation of the JAK-STAT and @entity28071 MAPK pathways in @entity291 myometrial cells. Inhibition of the @entity28071 pathway by the specific inhibitor @entity1441 strongly decreases @entity39979 mRNA and protein expression without affecting the phosphorylation and DNA-binding of transcription factors @entity2924 and @entity2925 . The @entity28071 pathway regulates @entity39979 expression at the posttranscriptional level, because the presence of @entity1441 results in the destabilization of @entity32589 -induced @entity39979 mRNA. Taken together, these data demonstrate the ability of @entity32589 to induce the activation of the JAK-STAT pathway in a manner similar to other types of IFN (i.e. alpha, beta, and gamma) and to regulate @entity39979 mRNA stability through the activation of the @entity28071 pathway. These findings provide new insights into the physiological function of @entity32589 , in regard to regulation of specific genes associated with myometrial contractility.
