De novo smoldering XXXX : a flow cytometric diagnosis.

@entity1 @entity17443 @entity417 @entity882 @entity1009 @entity8763 @entity2150 @entity7965 @entity2280 @entity9819 @entity3043
An asymptomatic 26-year-old @entity1 with mild @entity17443 (11.6 g Hb/dl) was studied. All biochemical parameters, bone marrow histology and cytogenetics were normal. The Ham's and sucrose tests were negative. A flow cytometric analysis revealed that @entity9819 and @entity1009 staining was absent in 20% and 21% of the granulocytes, but erythrocytes and @entity2280 -positive bone marrow cells were @entity9819 and @entity1009 positive. Seven months after the initial study, the @entity1 suffered an episode of @entity8763 , with mild @entity417 , moderate @entity882 and a weak positive sucrose lysis test. A new flow cytometric analysis disclosed an increased percentage of @entity9819 and @entity1009 negative granulocytes and a 25% of erythrocytes with an intermediate pattern of fluorescence after @entity1009 labelling. At fourteen months, a population of @entity9819 -deficient erythrocytes was detected and the Ham's test became positive. The present report is, to our knowledge, the first case of smoldering @entity7965 in a @entity1 with no previous @entity2150 , or evident @entity3043 . The diagnosis was established by flow cytometry of peripheral blood granulocytes, with apparently phenotipically normal progenitor cells in an early stage of the disease. Flow cytometry appears to be a useful tool in our knowledge of @entity7965 evolution.
