[Nuclear factor-kappa B activity at the early stage of @entity2806 and reperfusion in XXXX ].

@entity1991 @entity2806 @entity548 @entity19 @entity63 @entity3002 @entity1993
OBJECTIVE: To observe the activity of nuclear factor-kappa B( @entity1991 ) during reperfusion after temporary @entity2806 and to evaluate its effect. METHODS: The @entity2806 -reperfusion(I/R) model of @entity19 was established with ligating bilateral common carotid arteries and @entity548 for 0.3 ml in the tail. @entity3002 ( @entity3002 ) were recorded. Immunohistochemical technique was used to detect the activity of @entity1991 subunit P65 and the inhibitory factor-kappa B alpha( @entity1993 ) in cerebral cortex during different periods of @entity63 and reperfusion. Their mRNA expressions were also measured by RT-PCR. RESULTS: @entity1991 P65 activity significantly increased 30 minutes after the reperfusion, peaking at the 2nd hour, and remaining high within 24 hours (all P < 0.05). But the mRNA expression didn't change much; @entity1993 and its mRNA expression began to decrease at 30 minutes after the reperfusion, peaking at the 2nd hour after the reperfusion (P < 0.05) and then gradually restored. Positive correlation was found between @entity1991 P65 activity and @entity3002 (P < 0.05), while negative correlation was shown among @entity1993 , its mRNA, and @entity3002 (P < 0.05, respectively). CONCLUSION: Both activated @entity1991 and decreased @entity1993 exist in the neural tissues of @entity19 at the early stage of @entity2806 -reperfusion. The activated @entity1991 may be involved in the @entity63 -reperfusion injury.
