Differential Effects of Cold Atmospheric Plasma in the Treatment of XXXX .

@entity5032 @entity137 @entity5 @entity11 @entity463
OBJECTIVE: @entity5032 ( @entity5032 ) has recently been shown to selectively target @entity5 cells with minimal effects on normal cells. We systematically assessed the effects of @entity5032 in the treatment of @entity463 . METHODS: Three @entity11 cell lines, normal astrocytes, and endothelial cell lines were treated with @entity5032 . The effects of @entity5032 were then characterized for viability, @entity137 /apoptosis, and cell cycle effects. Statistical significance was determined with student's t-test. RESULTS: @entity5032 treatment decreases viability of @entity11 cells in a dose dependent manner, with the ID50 between 90-120 seconds for all @entity11 cell lines. Treatment with @entity5032 for more than 120 seconds resulted in viability less than 35% at 24-hours posttreatment, with a steady decline to less than 20% at 72-hours. In contrast, the effect of @entity5032 on the viability of NHA and HUVEC was minimal, and importantly not significant at 90 to 120 seconds, with up to 85% of the cells remained viable at 72-hours post-treatment. @entity5032 treatment produces both cytotoxic and apoptotic effects with some variability between cell lines. @entity5032 treatment resulted in a G2/M-phase cell cycle pause in all three cell lines. CONCLUSIONS: This preliminary study determined a multi-focal effect of @entity5032 on @entity11 cells in vitro, which was not observed in the @entity5 cell lines. The decreased viability depended on the treatment duration and cell line, but overall was explained by the induction of @entity137 , apoptosis, and G2/M pause. Future studies will aim at further characterization with more complex pre-clinical models.
