[Study on anti- XXXX effect and involved mechanism of Guizhi Fuling capsule and its active complex].

@entity32 @entity558 @entity635 @entity1421 @entity98 @entity8207 @entity637
The aim of this study was to investigate the anti-inflammatory effect of Guizhi Fuling capsule and its active complex (consistent of 15 active compounds) on @entity558 -induced RAW264. 7 cells. The effect of Guizhi Fuling capsule and its active complex on cell viability in RAW264. 7 cells were determined by @entity98 assay. The inhibitory effect of Guizhi Fuling capsule and active complex on the releasing of @entity635 , @entity637 and @entity1421 induced by @entity558 in RAW264. 7 cells was detected by ELISA assay. The expression of @entity635 and @entity8207 in Guizhi Fuling capsule or active complex treated RAW264. 7 cells was examined by Western blot assay. Guizhi Fuling capsule and active complex showed no significant effect on the cell viability in RAW264. 7 cells at doses range from 12.5 to 400 mg x L(-1). Compared with @entity558 treated group, Guizhi Fuling capsule and active complex dose dependently reduced the releasing of @entity635 , @entity637 and @entity1421 induced by @entity558 in RAW264. 7 cells. Moreover, the expression of @entity635 and @entity8207 was decreased after Guizhi Fuling capsule and active complex treatment, which might contribute to the inhibitory effect of Guizhi Fuling capsule in the releasing of @entity635 , @entity637 and @entity1421 . This study provided the evidence that Guizhi Fuling capsule and active complex remarkably inhibited the releasing of @entity635 , @entity637 and PGE2induced by @entity558 in RAW264. 7 cells by reducing the expression @entity635 and @entity8207 . This study provided an experimental basis of Guizhi Fuling capsule for the treatment of @entity32 and a theoretical basis for the development of effective compounds of Guizhi Fuling capsule.
