Changes in T lymphocyte subsets following injury. Assessment by flow cytometry and relationship to XXXX .

@entity1 @entity295 @entity19 @entity281 @entity9623 @entity204 @entity130
The increased susceptibility of severely injured @entity1 to @entity281 and @entity204 from @entity295 has been attributed to abnormalities in cell-mediated immunity. The authors therefore assessed the relative number of peripheral blood T helper cells and T suppressor/cytotoxic cells and total T lymphocytes identified by the monoclonal antibodies (McA) OKT4, OKT8, and OKT3, respectively, in 25 @entity1 with burns from 5 to 85% total body surface area (TBSA) (mean: 40%) and 21 @entity1 with nonthermal injuries (mean Injury Severity Score (ISS): 21.4). @entity1 were compared to 21 healthy controls. Cells reacting with the @entity9623 were detected by flow cytometry, which enabled the examination of a population of cells the size of T lymphocytes, excluding larger contaminating cells that might bind the McA. @entity1 with burns of 30% TBSA or greater had a significant reduction (p less than or equal to 0.05) in @entity19 + cells up to 50 days post-burn. Both septic and nonseptic burn @entity1 had reduced numbers of @entity19 + cells, as did @entity1 after nonthermal injury, suggesting that this reduction was due to the injury itself. @entity1 with smaller burns (less than 30% TBSA) as a group did not have reduced @entity19 + cells, whereas those with larger burns showed significant reductions in @entity19 + cells (P less than or equal to 0.05) at 0 to 5, 6 to 10, 11 to 20, 21 to 30, and 41 to 50 days post-burn. Seven burn @entity1 who became septic 10 days post-burn or later had significantly lower @entity19 + cells within 10 days of injury (mean: 33.75% +/- 7.4 SEM) than 10 @entity1 who remained free of @entity295 (mean: 42.2% +/- 5.4, p = 0.004). @entity1 with uncomplicated nonthermal injuries failed to show any significant reduction in @entity19 + cells. Following @entity130 , a reduction in @entity19 + cells was observed up to 10 days in @entity1 with burns less than 30% TBSA, and up to 20 days in @entity1 with larger burns. In both groups, at no time were increased @entity19 + cells found to correlate with clinical events. In @entity1 with nonthermal injury, @entity19 + cells generally remained near the normal range.
