In vivo and in vitro destruction of XXXX liver cytochrome P-450 by a monoterpene ketone, @entity44767 .

@entity23919 @entity5954 @entity44765 @entity1110 @entity35 @entity66642 @entity44767 @entity1446
In vivo administration of @entity44767 once daily decreased the levels of liver microsomal cyt. P-450 to the extent of 32 and 76% at the end of 24 and 96 hrs respectively. However, cyt. b5 and @entity1110 -cyt. c reductase activities remained unchanged. In vitro incubation (15 min) of liver microsomes from @entity66642 (PB)-treated @entity35 with @entity44767 (10 mM), aerobically or anaerobically resulted in the loss (approximately 60%) of cyt. P-450 in the presence or absence of @entity1110 . Destruction of cyt. P-450 was more in PB-treated microsomes as compared to @entity5954 (MC)-treated and control microsomes. The loss of cyt. P-450 was accompanied by a concomitant loss of microsomal @entity1446 . In contrast, @entity44765 or @entity23919 upon incubation with PB-induced microsomes resulted in the conversion (25-40%) of cyt. P-450 to cyt. P-420 without any loss of microsomal @entity1446 . The destructive process is irreversible, time dependent, linear upto a substrate concentration of 10 mM and follows first order kinetics.
