Hypermethylation of @entity5 -related genes in Tunisian XXXX with @entity356 : clinical and biological significance.

@entity2529 @entity1 @entity986 @entity5 @entity5962 @entity356 @entity15893 @entity2247 @entity1968
BACKGROUND: Promoter hypermethylation is an alternative mechanism of gene silencing in @entity5 including @entity356 ( @entity356 ). Its affects genes with crucial functions as @entity5 suppressor. METHODS: DNA methylation in the promoter of @entity986 , @entity2529 , @entity1968 receptor b (RARb2), @entity15893 , and @entity5962 genes was investigated in 79 Tunisian @entity1 with @entity356 using methylation-specific PCR. RESULTS: The methylation frequencies vary from 31.6% for @entity986 to 65.8% for RARb2. Hypermethylation of @entity2529 and @entity5962 was associated with @entity5 grade and age (P = 0.04 and 0.034) respectively, while hypermethylation of @entity15893 correlated with TNM stage (P = 0.027). The distribution of the methylated DNA at @entity986 , @entity2529 , and @entity5962 promoters were different in the intestinal and diffuse histotypes of @entity356 according to TNM. Moreover, the survival rate of @entity1 with @entity986 methylated status was shorter than that of @entity1 with the unmethylated status (P log rank = 0.009). On the other hand, the hypermethylation of RARb2 correlated with @entity2247 expression (P = 0.001). CONCLUSION: We showed that methylation of @entity986 is predictive of poor prognosis and could be a useful marker. Moreover, the association between RARb2 methylation and @entity2247 expression suggests a functional link between these two proteins in @entity356 .
