Detection of apoptotic cells in intestines from XXXX with and without @entity344 .

@entity32 @entity1641 @entity2806 @entity75 @entity262 @entity344 @entity1977 @entity1321
OBJECTIVE: To identify apoptosis in @entity1641 intestines and determine whether apoptosis is associated with @entity344 or a specific tissue layer of intestine. ANIMALS: 38 @entity1641 that underwent surgery or were euthanatized for small or large @entity1321 and 9 control @entity1641 euthanatized for reasons other than @entity344 or @entity262 . PROCEDURE: Specimens were collected at surgery from intestine involved in the @entity75 and distant to the @entity75 site or at necropsy from several sites including the @entity75 site. Histologic tissue sections were stained with H_E, and apoptosis was detected by use of the terminal deoxynucleotidyl transferase-mediated @entity1977 nick end labeling technique. The number of apoptotic cells per hpf was counted in the mucosa, circular muscle, longitudinal muscle, and serosa. RESULTS: Apoptotic nuclei were seen in all layers of intestine. An increased number of apoptotic cells was found in the circular muscle of the intestine from @entity1641 with simple obstruction, compared with strangulating obstruction or healthy intestine. Intestine distant from a primary strangulating lesion had higher numbers of apoptotic cells than did intestine distant from a @entity75 or intestine taken at the site of a strangulating or @entity75 . CONCLUSIONS AND CLINICAL RELEVANCE: Intestine from @entity1641 with obstructing or strangulating lesions in the small intestine and large colon had high numbers of apoptotic cells possibly because of @entity2806 and subsequent @entity32 . Whether substantial apoptosis affects intestinal function is not yet known.
