Significance of mutation in the D-loop region in XXXX .

@entity5 @entity1 @entity26 @entity356
AIM: To investigate the mutation in the D-loop region of mitochondrial DNA in @entity5 and its influence on the changes of reactive @entity26 species (ROS) and cell cycle. METHODS: The D-loop region was amplified by PCR and sequenced. Reactive @entity26 species and cell cycle were detected by flow cytometry in 40 specimens from @entity5 and adjacent normal tissues. According to the sequence results, @entity356 tissue was divided into mutation group and control group. Reactive @entity26 species, apoptosis and proliferation in the two groups were compared. RESULTS: Among the 40 @entity5 specimens, 21 mutations were identified in 12 @entity1 , the mutation rate being 30%. There were four microsatellite instabilities in the mutations. No mutation was found in the adjacent tissues. Reactive @entity26 species, apoptosis and proliferation in the mutation group were all significantly higher than those in the control group. CONCLUSION: Mutation in the D-loop region plays a role in the genesis and development of @entity5 .
