@entity5091 @entity3558 production in @entity1963 : possible association with XXXX development.

@entity1 @entity294 @entity5091 @entity1963 @entity3558 @entity1964
BACKGROUND/PURPOSE: The etiology of @entity1964 ( @entity1964 ) is unknown. Previous investigations have suggested that abnormal production of mucins may have an etiologic role. Recently, a series of @entity3558 genes have been identified. @entity5091 is the predominant @entity3558 expressed in @entity1 . The authors have shown previously in vitro that use of @entity5091 can prevent bacterial translocation. Based on this, it was hypothesized that those @entity1 with @entity1963 ( @entity1963 ) would have an abnormal production of @entity5091 compared with normal @entity1 . METHODS: Fresh stool specimens were collected from @entity1 with a diagnosis of @entity1963 (with or without @entity1964 ) and from age-matched control @entity1 . Protein was extracted, and @entity5091 was detected with Western blot analysis. @entity5091 protein expression was quantified by densitometry measurements. Results are expressed as mean density +/- SD. Statistical comparison was done with unpaired t tests, with P less than.05 being considered significant. RESULTS: @entity5091 expression was detected in all control @entity1 (mean density, 121 +/- 47). @entity5091 level was lowest in one @entity1 with a viral-induced @entity294 (density = 71). In those @entity1 with @entity1963 , levels of @entity5091 protein expression were significantly lower (P <.05) than controls (12 +/- 15 for all @entity1963 @entity1 ). Levels of @entity5091 were lowest (nondetectable) in 2 @entity1963 @entity1 who had clinical evidence of @entity1964 . CONCLUSIONS: @entity5091 production is markedly depressed in @entity1 with @entity1963 and is absent with @entity1964 . This decline in protein expression may result in a decrease in epithelial barrier function and be a predisposing factor in the development of @entity1964 .
