Evaluation of the contribution of @entity21740 and @entity39979 to the production of @entity1421 and PGF2 alpha in epithelial cells from XXXX endometrium.

@entity398 @entity62805 @entity1421 @entity2918 @entity32589 @entity3009 @entity39979 @entity1426 @entity21740 @entity291 @entity8726 @entity8228
In ruminants, the production of @entity2918 by the endometrium is critical for recognition of pregnancy. In the absence of an embryonic signal, luteolytic pulses of PGF(2 alpha) are released by the uterus. In contrast, the presence of a viable conceptus reduces the production of PGF(2 alpha) relative to @entity1421 and prevents luteolysis through the release of trophoblastic interferon ( @entity32589 ). Initially, it was thought that epithelial and stromal endometrial cells were specialized in the production of a single type of @entity3009 . However, purified cell populations of both types of cell can produce PGF(2 alpha) and @entity1421 ; therefore, selective production of PGF(2 alpha) and @entity1421 must be regulated within each type of cell. Two distinct @entity3009 synthases, @entity21740 and @entity39979 , are involved in @entity3009 production and each may catalyse the production of a different @entity3009 . This possibility was investigated in cultured epithelial cells from @entity291 endometrium. Cells were treated with @entity398 or @entity1426 , and expression of @entity21740 and @entity39979 proteins was monitored over time and correlated with @entity3009 accumulation. Cells were also treated with increasing doses of inhibitors of @entity21740 or @entity39979 (non-steroidal anti-inflammatory drugs; NSAIDs) with or without @entity1426 or @entity398 : @entity8726 (0-50 micromol l(-1)) was used as a non-selective inhibitor; @entity62805 (0-500 micromol l(-1)) was used as a @entity21740 inhibitor and @entity8228 (0-1 micromol l(-1)) was used as a @entity39979 inhibitor. After stimulation with @entity1426 or @entity398 , @entity3009 production and expression of @entity39979 protein were increased. All inhibitors were able to block basal and stimulated @entity3009 production. These results indicate that in endometrium most, if not all, @entity3009 production is probably processed through @entity39979 .
