@entity9695 -loaded nanoparticles for the potential coadjuvant treatment of XXXX .

@entity137 @entity263 @entity7050 @entity10124 @entity9695
UNASSIGNED: @entity9695 (NS)-loaded nanoparticles (NPNS) were prepared from @entity10124 ( @entity7050 ) and eventually coated with chitosan (NPNSCS). Nanoparticles (NP) were spherical with sizes 379  59nm for NPNS and 393  66nm for NPNSCS and zeta potentials of -15  3mV for NPNS to 10  4mV for NPNSCS, suggesting an efficient coating. Drug encapsulation rate was high (88  5% and 83  7% of added drug) for NPNS and NPNSCS, respectively. After NP washing and re-suspension, 98  2% and 99  1% of the drug initially entrapped remained associated to NP. NS was dispersed in amorphous state within the polymeric matrix. Two-fold dilution of NP with pH 7.4 PBS provoked no drug release. However, 30-40% NS was released after a 1/10 dilution. NPNSCS and NPNS diluted 1/100 reduced the encapsulated drug to around 30% and 70%, respectively. In contrast, 100% NS was released from NP under sink conditions in less than 2h. The permeability of free-NS (1-1.5*10(-5)cm/s) was compared with NPNS (NPNS=6.4-8.1*10(-6)cm/s and NPNSCS=5.5-7.0*10(-6)cm/s) using the PAMPA assay. The @entity137 of free-NS and NS in NP on model @entity263 cells PC-3 and DU-145 showed the highest cytotoxic effect with NPNSCS on PC-3 cells (IC50=89 M).
