Molecular and biological analysis of echovirus 9 strain isolated from a XXXX @entity1 .

@entity1 @entity3918 @entity1473 @entity6
The full-length infectious cDNA clone was constructed and sequenced from the strain @entity3918 of echovirus 9, which was recently isolated from a 6-week-old @entity1 at the clinical onset of type 1 @entity6 . Parallel with the @entity3918 , the full-length infectious cDNA clone of the prototype strain echovirus 9 Barty (Barty-INF), was constructed and sequenced. Genetic relationships of the sequenced echo 9 viruses to the other members of the @entity1 enterovirus type B species were studied by phylogenetic analyses. Comparison of capsid protein sequences showed that the @entity3918 was closely related to both prototype strains: Hill and Barty-INF. The only exception was the inner capsid protein VP4 where serotype specificity was not evident and the @entity3918 clustered with the strain Hill and the strain Barty-INF with echovirus 30 Bastianni. Likewise, the nonstructural protein coding region, P2P3, of @entity3918 was more similar to strain Hill than to strain Barty-INF. However, like echovirus 9 Barty, the @entity3918 contained the RGD-motif in the @entity1473 terminus of capsid protein VP1. By blocking experiments using an RGD-containing peptide and a polyclonal rabbit antiserum to the alpha(v)beta(3)-integrin, it was shown that this molecule works as a cellular receptor for @entity3918 . By using primary @entity1 islets, it was shown that the @entity3918 is capable of infecting insulin-producing beta-cells like the corresponding prototype strains did. However, only @entity3918 was clearly cytolytic for beta-cells. The infectious clones that were made allow further investigations of the molecular features responsible for the diabetogenicity of the @entity3918 .
