The induction of XXXX synthase and intestinal vascular permeability by endotoxin in the @entity35 .

@entity7792 @entity66 @entity1317 @entity534 @entity619 @entity7293 @entity842 @entity460 @entity35 @entity526
1. The effect of endotoxin ( @entity526 lipopolysaccharide) on the induction of @entity842 synthase (NOS) and the changes in vascular permeability in the colon and jejunum over a 5 h period have been investigated in the @entity35 . 2. Under resting conditions, a @entity460 -dependent @entity66 , determined by the conversion of radiolabelled @entity534 to @entity7792 , was detected in homogenates of both colonic and jejunal tissue. 3. Administration of endotoxin (3 mg kg-1, i.v.) led, after a 2 h lag period, to the appearance of @entity460 -independent NOS activity in the colon and jejunum ex vivo, characteristic of the inducible NOS enzyme. 4. Administration of endotoxin led to an increase in colonic and jejunal vascular permeability after a lag period of 3 h, determined by the leakage of radiolabelled albumin. 5. Pretreatment with @entity619 (1 mg kg-1 s.c., 2 h prior to challenge) inhibited both the induction of NOS and the vascular leakage induced by endotoxin. 6. Administration of the NO synthase inhibitor @entity7293 (12.5-50 mg kg-1, s.c.) 3 h after endotoxin injection, dose-dependently reduced the subsequent increase in vascular permeability in jejunum and colon, an effect reversed by @entity534 (300 mg kg-1, s.c.). 7. These findings suggest that induction of NOS is associated with the @entity1317 induced by endotoxin in the @entity35 colon and jejunum.
