Epigenetic inactivation of @entity986 /CDK/ @entity995 cell cycle pathway in XXXX .

@entity7818 @entity1 @entity981 @entity986 @entity9288 @entity3052 @entity17342 @entity1778 @entity1349 @entity995
Dysregulation of cell cycle is important in oncogenesis. We analyzed the inactivation of the @entity986 family @entity17342 /CDK/ @entity995 pathway by gene promoter hypermethylation in leukemogenesis. The methylation-specific polymerase chain reaction (MSP) with primers for methylated (M-MSP) and unmethylated (U-MSP) alleles of the @entity9288 , @entity986 , @entity7818 , and @entity995 genes was used to study five leukemic cell lines, 50 @entity3052 ( @entity3052 ) and 25 @entity981 ( @entity981 ) samples. None of the leukemic cell lines showed @entity7818 and @entity995 methylation. @entity9288 was methylated in Raji, while @entity986 was methylated in U937 and Raji. In NB4 and Jurkat, both alleles of @entity9288 and @entity986 appeared to be deleted. At diagnosis, @entity9288 methylation occurred in 29 (58%) @entity3052 @entity1 , and 10 (40.0%) @entity981 @entity1 . @entity986 methylation occurred in two (4%) @entity3052 and two (8%) @entity981 @entity1 . Only one each of @entity3052 and @entity981 @entity1 had concurrent @entity9288 and @entity986 methylation. None of the @entity1 had methylation of @entity7818 or @entity995 . In @entity3052 , @entity9288 methylation was associated with M2 subtype ( p=0.018). @entity1 with and without @entity9288 methylation had similar complete remission (CR) rates and projected 5-year @entity1349 ( @entity1349 ) or disease-free survival (DFS). Therefore, methylation inactivation of the @entity986 /CDK/ @entity995 pathway in @entity1778 involved primarily @entity9288 and occasionally @entity986 , but not @entity7818 or @entity995 . In @entity3052 , @entity9288 gene methylation was associated with the M2 subtype, but was not prognostic for CR, @entity1349 , or DFS.
