Unique cytokine production profile of anergic XXXX T cells in @entity915 -hu @entity19 after staphylococcal enterotoxin B administration.

@entity1 @entity19 @entity310 @entity8282 @entity915 @entity379 @entity2902 @entity1199 @entity688 @entity1361 @entity4386
Severe combined immunodeficient @entity19 transplanted with @entity1 organs ( @entity915 -hu @entity19 ), provide a unique in vivo model for studying @entity1 intrathymic T cell selection and development of tolerance. In vivo administration of staphylococcal enterotoxin B (SEB) to @entity915 -hu @entity19 causes intrathymic clonal deletion of SEB-specific V beta+ T cells that occurs already at the immature CD4+8+ double positive stage. The expression of activation markers such as @entity2902 , @entity8282 , and HLA-DR was specifically increased on V beta+ T cells responding to SEB. The remaining SEB-specific @entity1 T cells that had not been deleted in vivo failed to proliferate when rechallenged with SEB in vitro. These SEB-specific T cells that were rendered anergic in vivo had a unique cytokine production profile. They failed to produce @entity1199 , which correlated with the lack of proliferation of these cells. In addition, they failed to produce @entity310 . However, the anergized T cells synthesized considerable amounts of @entity379 , granulocyte-macrophage CSF and @entity688 after SEB stimulation. This clonal anergy can be completely reversed in vitro by stimulating the SEB-specific cells in the presence of exogenous @entity1199 or by triggering of the @entity1361 / @entity4386 activation pathway. Under these stimulation conditions, anergic T cells produced levels of @entity1199 and @entity310 that were comparable to their non-anergized counterparts, whereas the levels of granulocyte-macrophage CSF, @entity688 and @entity379 production were even higher. Collectively, these data demonstrate that in vivo administration of SEB to @entity915 -hu @entity19 leads to activation, deletion, and anergy of SEB-specific @entity1 thymocytes and that the production of @entity1199 and @entity310 is selectively switched off in these anergic T cells.
