XXXX deficiency does not prevent local immune responses to adenovirus vector in islet transplant recipients.

@entity32 @entity13945 @entity6 @entity19 @entity13766 @entity1961 @entity164 @entity413
BACKGROUND: @entity13945 ( @entity13945 ) plays an important role in mediating @entity32 . We examined the effect of @entity13945 on the immune response to adenovirus vectors and graft function in islet transplant model. MATERIALS AND METHODS: Syngeneic @entity164 ( @entity164 ) or @entity13945 -deficient (KO) @entity19 islets transduced with adenovirus encoding b-gal were transplanted under the renal capsule. After transplant, blood @entity413 , @entity413 tolerance, graft cellular infiltration, transgene and chemokine/receptor expression, and systemic anti-adenoviral/-b-gal immune response were evaluated. RESULTS: @entity6 was reversed in 1 d in both @entity164 and KO untransduced recipients, while islets transduced with adenovirus failed to reverse @entity6 until 10  d post-transplant in @entity164 recipients or even longer (>15  d) in KO recipients (P < 0.05). A profound infiltration of @entity1961 (+), CD8(+) cells and macrophages was observed in both @entity164 and KO transduced grafts at 25 d. Though transgene expression was significantly reduced, insulin and b-gal expression persisted over 3  mo. @entity413 tolerance was impaired in all grafts in KO recipients compared with untransduced grafts in @entity164 recipients at 25 d post-transplant, but was equivalent at 3 mo. Early expression of @entity13766 mRNA was increased in transduced grafts in both @entity164 and KO recipients. No systemic antivector immunity was demonstrated in any recipient group. CONCLUSIONS: Transduction of islets with adenovirus causes significant @entity32 in islet grafts and impairs early graft function in @entity13945 -deficient recipients, but long-term graft function is preserved. Thus, @entity13945 absence does not prevent the local immune response to adenovirus transduction, and vector-associated graft dysfunction is not mediated by @entity13945 .
