Regional myocyte size in two-kidney, one clip renal XXXX .

@entity101 @entity575 @entity1283 @entity3584 @entity3652 @entity4346 @entity460 @entity35
Regional variations in the size and shape of isolated myocytes were studied using the two-kidney, one clip (2K1C) renal model of @entity101 . Weanling male @entity35 (50 to 75 g) were anesthetized by @entity3652 (100 mg/kg) during renal artery clipping (0.2 mm internal diameter @entity575 clip) and were then allowed to grow for 6 to 8 weeks, when the blood pressure had stabilized at 180 mmHg. Hearts were removed, weighed and then were perfused with a @entity460 -free Joklik medium containing collagenase. Isolated myocytes were collected from five regions and fixed in isoosmolar @entity3584 : right ventricular free wall (RVFW), right and left halves of the interventricular septum (RIVS, LIVS), and epicardial and endocardial halves of the left ventricular free wall (LEPI, LENDO). Myocyte volume was measured by Coulter Counter. Myocyte length was measured by sonic digitizer. Cross-sectional area was calculated from myocyte volume and length. Tailcuff systolic pressure and heart weight were significantly increased in 2K1C @entity35 as compared to control. Body weights were not different. Cell volume was significantly increased in RIVS, LIVS, LEPI, and LENDO, but not in RVFW. Cell length was not significantly increased in any region. Thus, the 2K1C model showed a predominant @entity4346 in which the right half of the septum acted in concert with the left ventricle. The shape of the hypertrophied myocytes, having an increase in volume due to an increase in cross-sectional area but not length, was most consistent with a pressure-induced form of @entity1283 .
