Effects of nuclear factor-kB and @entity3963 signaling transduction pathway inhibitors on @entity1 XXXX cell proliferation in vitro.

@entity1 @entity5732 @entity588 @entity3963 @entity2454 @entity36607 @entity320
UNASSIGNED: The present study aimed to investigate the effects of blocking nuclear factor (NF)-kB and/or @entity3963 ( @entity3963 ) signaling pathways on proliferation and apoptosis of @entity320 cells in vitro. A375 @entity1 @entity320 cells were treated with @entity5732 ( @entity3963 signaling pathway inhibitor) and @entity36607 (NF-kB inhibitor), alone or in combination. At 12, 24 and 48 h after treatment, cell viability was assessed using the @entity2454 assay, cell cycle progression and apoptosis were evaluated by flow cytometry, and @entity588 protein content was determined by western blot analysis. @entity36607 and @entity5732 significantly inhibited A375 cell proliferation in a dose- and time-dependent manner (P<0.01). The rate of proliferation inhibition at 24 h was 35.41  1.38% for @entity36607 alone, 30.64  2.86% for @entity5732 alone, and 77.27  2.70% for @entity36607 and @entity5732 in combination. The difference between combination and single treatment was significantly different (P<0.01). The proportion of cells in S phase was 14.20, 18.40 and 22.64% following treatment with @entity36607 , @entity5732 , and @entity36607 and @entity5732 in combination, respectively; these values were all significantly reduced compared with the untreated control group (P<0.01). The apoptosis rate was 24.98  1.03% in the @entity36607 group, 13.96  0.96% in the @entity5732 group and 38.91  1.46% in the combination group; all significantly increased compared with the control group (P<0.01). @entity588 protein content in A375 cells was significantly increased following treatment with @entity36607 and @entity5732 , alone or in combination, when compared with the untreated control group (P<0.01). Therefore, NF-kB and @entity3963 signaling pathway inhibitors may serve as potential therapeutic targets for @entity320 .
