Inhibition of MicroRNA 195 Prevents Apoptosis and Multiple-Organ Injury in XXXX Models of @entity295 .

@entity1 @entity14074 @entity295 @entity19 @entity651 @entity1379 @entity8627 @entity14075 @entity204
BACKGROUND: MicroRNAs (miRs) are a class of short RNA molecules, which negatively regulate gene expression. The levels of circulating miR-15 family members are elevated in septic @entity1 and may be associated with @entity204 . This study investigated whether inhibition of @entity14074 , a member of the miR-15 family, provided beneficial effects in @entity295 . METHODS AND RESULTS: @entity295 was induced by injection of feces into the peritoneum in @entity19 . @entity14074 was upregulated in the lung and liver of septic @entity19 . Silencing of @entity14074 increased the protein levels of @entity1379 , @entity14075 , and @entity8627 ; prevented apoptosis; reduced liver and @entity651 ; and improved the survival in septic @entity19 . Silencing of @entity14074 provided similar protection in lipopolysaccharide-induced endotoxemic @entity19 . In endothelial cells, upregulation of @entity14074 induced apoptosis, and inhibition of @entity14074 prevented lipopolysaccharide-induced apoptosis. @entity14074 repressed expression of its protein targets, @entity1379 , @entity14075 , and @entity8627 . Furthermore, overexpression of @entity8627 prevented apoptosis induced by lipopolysaccharide and @entity14074 mimic. Inhibition of @entity8627 attenuated the protective effects of @entity14074 silencing in septic @entity19 . CONCLUSIONS: Silencing of @entity14074 reduced multiple-organ injury and improved the survival in @entity295 , and the protective effects of @entity14074 inhibition were associated with upregulation of @entity1379 , @entity14075 , and @entity8627 . Thus, inhibition of @entity14074 may represent a new therapeutic approach for @entity295 .
