MULTINDELS-BOV: XXXX traceback method based on DNA insertion-deletion polymorphisms.

@entity2226 @entity291 @entity7040
Brazil is a major producer and exporter of beef, with a herd of approximately 210 million animals. For the meat industry, a reliable animal traceback from its origin to the consumer market is paramount. Of all available identification systems, DNA is the only one that survives the slaughterhouse and reaches the dish of the consumer. DNA polymorphisms are already used for @entity291 traceback, but primarily for the subspecies @entity291 taurus. However, in Brazil, another subspecies, @entity7040 predominates. We describe here the development of a DNA traceback method designed primarily for @entity7040 ( @entity7040 ), without leaving @entity291 taurus aside. We used insertion/deletion (indel) polymorphisms, which have the advantage of being simple and easily automatable, since in most cases, the variable loci are biallelic. We studied 94 indels, with a difference of two or more base pairs, in DNA pools of 60 @entity7040 and 60 @entity2226 animals. A set of 22 indels with heterozygosity greater than 0.3 were selected and used to construct two multiplex PCRs. On the basis of the allelic frequency of these indels, the probability of random match was calculated to be 1.12 x 10(-8) for @entity7040 and 1.60 x 10(-6) for @entity291 taurus. Moreover, we estimated that an analysis would cost less than US 15.00 per animal. Thus, this system (MULTINDELS-BOV) is perfectly suited for building large genetic databases and offering viable prospects of a national system for @entity291 traceback DNA in Brazil.
