A multiresistant clone of Shiga toxin-producing @entity526 O118:[H16] is spread in @entity291 and XXXX over different European countries.

@entity1 @entity801 @entity18585 @entity7913 @entity42545 @entity11682 @entity2177 @entity377 @entity3639 @entity2755 @entity802 @entity206 @entity64596 @entity869 @entity803 @entity291 @entity526
Multiresistant Shiga toxin-producing @entity526 (STEC) O118:H16 and O118 nonmotile strains (designated O118:[H16]) were detected by examination of 171 STEC isolates for their antimicrobial sensitivity. Of 48 STEC O118:[H16] strains, 98% were resistant to @entity11682 , 96% were resistant to @entity3639 , 79% were resistant to @entity7913 , 75% were resistant to @entity803 , 67% were resistant to @entity869 , 60% were resistant to @entity2755 , 48% were resistant to @entity206 , and 10% each were resistant to @entity801 and @entity802 . @entity802 resistance and reduced susceptibility to @entity2177 were associated with the mutation gyrA(LEU-83). The STEC O118:[H16] strains were found to belong to a single genetic clone as investigated by multilocus enzyme electrophoresis and by multilocus sequence analysis of @entity526 housekeeping genes. The STEC O118:[H16] strains originated from @entity1 and @entity291 and were isolated in seven different countries of Europe between 1986 and 1999. Strains showing multiresistance to up to eight different antimicrobials predominated among the more recent STEC O118:[H16] strains. The genes in parentheses were associated with resistance to @entity7913 (aphA1-Ia), @entity2755 ( @entity64596 ), @entity803 [tet(A)], and @entity869 (bla( @entity18585 )). Class 1 integrons containing sulI ( @entity11682 resistance), aadA1a ( @entity3639 resistance), or @entity42545 ( @entity206 resistance)-aadA1a gene cassettes were detected in 28 strains. The bla(TEM-1b) gene was present in 18 of 21 strains that were examined by @entity377 sequencing. Class 1 integrons and bla(TEM) genes were localized on plasmids and/or on the chromosome in different STEC O118:[H16] strains. Hybridization of XbaI-digested chromosomal DNA separated by pulsed-field gel electrophoresis revealed that bla(TEM) genes were integrated at different positions in the chromosome of STEC O118:[H16] strains that could have occurred by Tn2 insertion. Our data suggest that strains belonging to the STEC O118:[H16] clonal group have a characteristic propensity for acquisition and maintenance of resistance determinants, thus contrasting to STEC belonging to other serotypes.
