@entity59292 expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed XXXX upon Leishmania chagasi promastigote stimulation in vitro.

@entity1 @entity19 @entity235 @entity59292 @entity14121 @entity281 @entity33781
While the response to Leishmania spp. is well characterized in @entity19 and @entity1 , much less is known concerning the @entity235 immune response, particularly soon after exposure to the parasite. Early events are considered to be a determinant of @entity281 outcome. To investigate the @entity235 's early immune response to L. chagasi, an in vitro priming system (PIV) using @entity235 na  ve PBMC was established. Until now, @entity235 PIV immune response to L. chagasi has not been assessed. We co-cultivated PBMC primarily stimulated with L. chagasi in vitro with autologous infected macrophages and found that @entity59292 mRNA is up-regulated in these cells compared to control unstimulated cells. @entity33781 and @entity14121 mRNA expression by L. chagasi-stimulated PBMC was similar to control unstimulated PBMC when incubated with infected macrophages. Surprisingly, correlation studies showed that a lower @entity59292 / @entity33781 expression ratio correlated with a lower percentage of @entity281 . We propose that the direct correlation between @entity59292 / @entity33781 ratio and parasite load is dependent on the higher correlation of both @entity59292 and @entity33781 expression with lower parasite @entity281 . This PIV system was shown to be useful in evaluating the @entity235 immune response to L. chagasi, and results indicate that a balance between @entity59292 and @entity33781 is associated with control of parasite @entity281 in vitro.
