Immunolocalization of ABC drug transporters in XXXX placenta from normal and gestational @entity6 .

@entity1 @entity2907 @entity28671 @entity6 @entity1885 @entity645 @entity3970 @entity11092 @entity7895
OBJECTIVES: ABC transporters, @entity7895 , @entity3970 , @entity28671 and @entity11092 , can bind both endo- and exogenous ligands. The latter include immunosuppressive, anticancer sedative, anticonvulsant, antiparasitic and cardiovascular drugs, as well as @entity645 protease inhibitors and antibiotics. Protein transporters are also involved in tissue distribution of orally administered medicines in combination therapy for @entity6 ( @entity6 ) and could be used during @entity6 treatment. The distribution depends on transporter specificity its expression and subcellular localization. THE AIM: The aim of the study was to compare @entity7895 , @entity3970 , @entity28671 and @entity11092 localization in placental tissues from normal and @entity6 . MATERIAL AND METHODS: Tissue samples were taken from 10 normal and 10 @entity6 placentas. Immunohistochemical reactions were performed with the use of adequate monoclonal antibodies. Avidin- @entity1885 -peroxidase complex method was used for the visualization of antigen-antibody complexes. RESULTS: @entity7895 , @entity3970 and @entity28671 were found in all parts of normal @entity1 placenta i.e. the @entity2907 epithelium, cytotrophoblast, syncytiotrophoblast and decidual cells. @entity7895 and @entity28671 , but not @entity3970 and @entity11092 , were also localized on the endothelial cells of fetal blood vessels in the chorionic plate, as well as stem and tertiary villi. @entity11092 expression was observed in the cytotrophoblast and the syncytiotrophoblast. Its expression was very low or undetectable in the @entity2907 epithelium and the majority of decidual cells. Immunohistochemical reactions within the syncytiotrophoblast showed apical ( @entity7895 , @entity28671 ), apical and basal ( @entity11092 ) or diffuse ( @entity3970 ) distribution. The main changes observed in @entity6 placentas included weaker @entity11092 and @entity3970 positive reactions in the syncytiotrophoblast, slightly lower expression of @entity7895 in the decidual and @entity2907 epithelial cells, and @entity3970 in the @entity2907 epithelium. CONCLUSIONS: Our results indicate that @entity6 -related changes in the environment of placental cells do not substantially influence tissue and subcellular location of ABC transporters. Nevertheless, the expression of @entity7895 , @entity3970 and @entity11092 may be lower in comparison to normal placentas. Basal syncytiotrophoblast transporters, @entity11092 and @entity3970 , seem to be more sensitive to the influence of @entity6 than apical proteins, what may result in altered biodisposition of endogenous substrates and drugs.
