Activity-induced XXXX -dependent MRI (AIM-MRI) and functional MRI in awake @entity95 during somatosensory stimulation.

@entity921 @entity3281 @entity13718 @entity95 @entity460
UNASSIGNED: Activity-induced @entity921 -dependent MRI (AIM-MRI) is a powerful tool to track system-wide neural activity using high resolution, quantitative T1-weighted MRI in animal models and has significant advantages for investigating neural activity over other modalities including BOLD fMRI. With AIM-MRI, @entity3281 ions enter neurons via voltage-gated @entity460 channels preferentially active during the time of experimental exposure. A broad range of AIM-MRI studies using different species studying different phenomena have been performed, but few of these studies provide a systematic evaluation of the factors influencing the detection of @entity3281 such as dosage and the temporal characteristics of @entity3281 uptake. We identified an optimal dose of @entity3281 (25mg/kg, s.c.) in order to characterize the time-course of @entity3281 accumulation in active neural regions in the @entity95 . T1-weighted MRI and functional MRI were collected 0-3, 6-9, and 24-27h post- @entity3281 injection while the vibrissae on the right side were vibrated. Significant BOLD activation in the left somatosensory (SS) cortex and left ventral posteromedial (VPM) thalamic nucleus was detected during whisker vibration. T1-weighted signal intensities were extracted from these regions, their corresponding contralateral regions and the visual cortex (to serve as controls). A significant elevation in T1-weighted signal intensity in the left SS cortex (relative to right) was evident 6-9 and 24-27h post- @entity3281 injection while the left VPM thalamus showed a significant enhancement (relative to the right) only during the 24-27h session. Visual cortex showed no hemispheric difference at any timepoint. Our results suggest that studies employing AIM-MRI would benefit by conducting experimental manipulations 6-24h after subcutaneous @entity13718 injections to optimize the concentration of contrast agent in the regions active during the exposure.
