XXXX and gelatinolytic activity of a new silicon-based endodontic sealer.

@entity98 @entity137 @entity1495 @entity1476
PURPOSE: To compare the @entity137 , gelatinolytic activity, and protein levels ( @entity1476 and @entity1495 ) produced by 3T3 fibroblasts cells after stimulation with GuttaFlow 2 and AH Plus. METHODS: 3T3 fibroblasts were incubated with elutes of GuttaFlow 2 and AH Plus for 24 h. The @entity137 of tested materials was determined using the @entity98 and the LDH assay. Supernatants of cell cultures incubated with sealers were collected to determine the levels of @entity1476 and @entity1495 gelatinolytic activity by gelatin zymography. Cell lysates were used to determine @entity1476 and @entity1495 protein levels by Western Blot. Data were analyzed using ANOVA and Tukey test (P<0.05). RESULTS: AH Plus showed significantly less cell viability (mitochondrial activity of cells) than GuttaFlow 2 (P<0.01). Moreover, GuttaFlow 2 was noncytotoxic, showing no statistically significant difference in LDH leakage levels compared to the control group (P>0.05). Specific characterization of MMPs demonstrated that GuttaFlow 2 seemed not to affect @entity1476 levels compared with the control group, while AH Plus had elevated gelatinolytic activity and protein levels of @entity1476 as confirmed by quantitative measurements. No detectable gelatinolytic activity or protein levels of @entity1495 (92 kDa) was observed in any tested group. CONCLUSIONS: GuttaFlow 2 did not showed cytotoxic effects and did not induce @entity1476 or @entity1495 expression.
