Anti-inflammatory properties of Ajuga bracteosa in vivo and in vitro study and their effects on XXXX model of @entity1575 .

@entity32 @entity1992 @entity1991 @entity14605 @entity19 @entity3636 @entity5751 @entity1575 @entity99 @entity637
ETHNOPHARMACOLOGICAL RELEVANCE: The entire plant of Ajuga bracteosa Wall has been used to treat various @entity32 , including @entity99 , in Taiwan. AIM: This study evaluated the hepatoprotective ability of Ajuga bracteosa extract (ABE). MATERIALS AND METHODS: We investigated the inhibitory action of a @entity3636 fraction of ABE (ABCE) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells and Kupffer cells. @entity1575 was induced in @entity19 through the administration of @entity14605 twice a week for 8 weeks. @entity19 in three @entity14605 groups were treated daily with water and ABE throughout the duration of the experiment. RESULTS: In LPS-stimulated RAW264.7 cells and Kupffer cells, ABCE inhibited the production of NO and/or @entity637 and also blocked the LPS-induced expression of NO synthase. ABCE inhibited the activation of NF-kB induced by LPS, associated with the abrogation of IkBa degradation, with a subsequent decrease in nuclear @entity1992 and @entity1991 protein levels. The phosphorylation of MAPKs in LPS-stimulated RAW264.7 cells was also suppressed using ABCE. In the in vivo study, ABE protected the liver from injury by reducing the activity of plasma aminotransferase, and by improving the histological architecture of the liver. RT-PCR analysis showed that ABE inhibited the hepatic mRNA expression of LPS binding protein, @entity5751 , @entity637 , collagen(a1)(I), and a-smooth actin. CONCLUSION: These results indicate that ABE alleviated @entity14605 -induced @entity1575 , and that this protection is probably due to the suppression of macrophage activation.
