Targeting @entity16262 and @entity517 expression by microRNA-17-5p represses interferon-stimulated gene XXXX in peripheral blood mononuclear cells of pediatric systemic lupus erythematosus @entity1 .

@entity1 @entity16262 @entity10014 @entity12425 @entity517 @entity13434 @entity3027
OBJECTIVES: Elevated @entity3027 is believed to be one of the crucial factors involved in the pathogenesis of systemic lupus erythematosus (SLE). Its expression was recently found to be governed by the transcription factor @entity16262 which is involved in an autoregulatory triad along with @entity517 and the microRNA polycistron @entity10014 . However, this intricate triad has seldom been investigated in SLE @entity1 . Therefore, the current study was undertaken to investigate the expression pattern of the @entity16262 / @entity517 / @entity12425 triad in peripheral blood of SLE @entity1 as well as to examine the impact of manipulating this triad using @entity12425 mimics and inhibitors on @entity3027 signature in SLE @entity1 . METHODS: Expression of the @entity16262 / @entity517 / @entity12425 triad and the @entity3027 -stimulated gene @entity13434 was analyzed using real time qPCR. Peripheral blood mononuclear cells from SLE @entity1 and controls were transfected with @entity12425 mimics and antagomirs using the HiPerfect transfection reagent. RESULTS: @entity16262 transcripts and @entity12425 were significantly downregulated while @entity517 and @entity13434 transcripts were significantly upregulated in SLE. Also, transfection of SLE PBMCs with @entity12425 mimics led to a substantial repression of @entity16262 and @entity517 expression. The overall change in this triad upon @entity12425 mimicking resulted in lowering the transcript levels of the @entity3027 -inducible gene @entity13434 in SLE. CONCLUSION: This may advocate the manipulation/use of the @entity16262 / @entity517 / @entity12425 trinity to effectively control the aberrantly high levels of type I @entity3027 activity in lupus @entity1 .
