Effect of Nigella sativa (N. sativa L.) and oxidative stress on the survival pattern of MCF-7 XXXX cells.

@entity137 @entity274 @entity0 @entity101 @entity6 @entity5 @entity167 @entity1068
N. Sativa L., an oriental spice, has long been used as a natural medicine for treatment of many acute as well as chronic conditions. It has been used in the treatment of @entity6 , @entity101 , and dermatological conditions. There has been very few studies on the effects of N. Sativa as @entity5 prevention/therapy. Our objective therefore, was to expose MCF-7 @entity0 cells to aqueous and @entity167 extracts and in combination with @entity274 as an oxidative stressor. Measurement of cell survival under various concentrations and combinations was conducted using standard cell culture techniques, exposure protocols in 96 well plates and Fluoro-spectrosphotometry. Following cellular growth to 90% confluency, exposure to water (WE) and @entity1068 (AE) extracts of N. sativa and @entity274 was performed. @entity137 index (LC50) was calculated from percent survival using regression analysis. Results showed that the @entity167 extract and its combinations were able to completely inactivate the MCF-7 cells (LC50 ranged from 377.16-573.79 in descending potency for @entity274 + AE, AE and Mix of WE and AE). @entity274 alone effectively inactivated MCF-7 cells (LC50 = 460.94). The least effective combinations in descending potency were WE + @entity274 , WE + AE + @entity274 , and WE (LC50 were 725.79, 765.94, and 940.5 respectively. Combinations other than AE + @entity274 showed possible interactions, which lead to reduction in their potency. In conclusion, N. Sativa alone or in combination with oxidative stress were found to be effective in vitro in inactivating MCF-7 @entity0 cells, unveiling opportunities for promising results in the field of prevention and treatment of @entity5 .
