Antigen-Specific XXXX ameliorates allergic airway inflammation via Fcy receptor IIB on dendritic cells.

@entity10328 @entity19 @entity565 @entity7097 @entity9686 @entity1358 @entity13446 @entity941 @entity11805 @entity942
BACKGROUND: There have been few reports on the role of Fc receptors (FcRs) and @entity13446 ( @entity13446 ) in @entity565 . The purpose of this study is to clarify the role of inhibitory FcRs and antigen presenting cells (APCs) in pathogenesis of @entity565 and to evaluate antigen-transporting and presenting capacity by @entity1358 . METHODS: In @entity942 (KO) and C57BL/6 ( @entity7097 ) @entity19 , the effects of intratracheal instillation of antigen-specific @entity13446 were analysed using the model with sensitization and airborne challenge with @entity10328 (OVA). Thoracic lymph nodes instilled with @entity941 -conjugated OVA were analysed by fluorescence microscopy. Moreover, we analysed the @entity11805 + MHC class II+ cells which intaken @entity941 -conjugated OVA in thoracic lymph nodes by flow cytometry. Also, lung-derived @entity11805 + APCs were analysed by flow cytometry. Effects of anti-OVA @entity9686 on bone marrow dendritic cells (BMDCs) in vitro were also analysed. Moreover, in FcyRIIB KO @entity19 intravenously transplanted dendritic cells (DCs) differentiated from BMDCs of @entity7097 @entity19 , the effects of intratracheal instillation of anti-OVA @entity13446 were evaluated by bronchoalveolar lavage (BAL). RESULTS: In @entity7097 @entity19 , total cells and eosinophils in BAL fluid reduced after instillation with anti-OVA @entity9686 . Anti-OVA @entity9686 suppressed airway inflammation in hyperresponsiveness and histology. In addition, the number of the @entity941 -conjugated OVA in @entity11805 + MHC class II+ cells of thoracic lymph nodes with anti-OVA @entity9686 instillation decreased compared with PBS. Also, MHC class II expression on lung-derived @entity11805 + APCs with anti-OVA @entity9686 instillation reduced. Moreover, in vitro, we showed that BMDCs with anti-OVA @entity9686 significantly decreased the T cell proliferation. Finally, we demonstrated that the lacking effects of anti-OVA @entity9686 on airway inflammation on FcyRIIB KO @entity19 were restored with @entity7097 -derived BMDCs transplanted intravenously. CONCLUSION: Antigen-specific @entity13446 ameliorates allergic airway inflammation via FcyRIIB on DCs.
