Involvement of the XXXX necrosis factor receptor @entity10733 in mediating @entity137 and gene regulating activities.

@entity1 @entity137 @entity2451 @entity5 @entity10733 @entity2834 @entity980 @entity10736
Using agonistic antibodies (Ab) we have examined whether the 75-kDa chain of the @entity5 necrosis factor receptor @entity10733 ) is capable of mediating cytotoxic response and gene regulation alone or in cooperation with @entity10736 @entity10736 . Addition of an anti- @entity10733 polyclonal antiserum or anti- @entity10733 monoclonal antibody (mAb) plus anti-immunoglobulin (Ig) led to cytotoxic response of @entity1 KYM-1 @entity980 cells. Anti- @entity10733 mAb alone had no effect pointing out the importance of strong receptor stimulation for signal transduction into the cell. Simultaneous triggering of both the @entity10736 and @entity10733 @entity10736 by agonistic Ab resulted in additive cytotoxic action on KYM-1 cells. The anti- @entity10733 mAb 3H5, directed to a non-TNF binding site on the @entity1 @entity10733 , was used to confirm further the ability of the @entity10733 to potentiate @entity10736 @entity10736 -mediated cell death. While exhibiting no @entity137 by its own, 3H5 significantly augmented the cytotoxic effect of the anti- @entity10736 mAb htr9 towards KYM-1 cells. Neither the anti- @entity10733 @entity10736 antiserum nor anti- @entity10733 mAb were cytotoxic for @entity1 U937 cells suggesting that the cytolysis resulting from @entity10733 cross-linking is cell specific. Noteworthy, stimulation of the @entity10733 @entity10736 with mAb plus anti-Ig or polyclonal antiserum led to a marked enhancement of the @entity10736 @entity10736 -induced U937 cell death, indicating collaboration between the two @entity10736 in induction of @entity137 also in this cell line. However, 3H5 mAb did not affect the ability of anti- @entity10736 mAb to lyse U937 cells. Altogether, these data demonstrate the difference between KYM-1 and U937 cell lines with respect to the role for the @entity10733 in mediating @entity137 . Both @entity10736 were found to mediate cytomegalovirus ( @entity2834 ) promoter activation in @entity1 SW480T-beta Gal cells and @entity2451 ( @entity2451 ) induction in this cell line as well as in KYM-1 cells. It was demonstrated for the first time that independent stimulation of both @entity10736 resulted in an additive effect on the @entity2834 promoter activation and induction of the @entity2451 . Taken together, these results indicate that the @entity10733 induces @entity137 in a cell-specific manner and potentiates @entity10736 @entity10736 -mediated cytotoxic response and gene regulation.
