Monitoring @entity1014 kinase and @entity4540 -induced changes in XXXX malignant @entity11 in vivo by nuclear magnetic resonance imaging.

@entity5 @entity11 @entity1014 @entity35925 @entity35 @entity4540 @entity70586
We have used high resolution magnetic resonance imaging to monitor malignant @entity35 BT4C @entity11 in vivo following herpes simplex virus @entity1014 kinase gene and @entity4540 (GCV) treatment. Twenty-six female BDIX @entity35 were used for the study including four controls. Serial magnetic resonance imaging was performed every 72 hours to quantify @entity5 volume, transverse relaxation time (T2) ,and apparent diffusion constant ( @entity35925 ) of water in the @entity5 and in the contralateral brain. GCV treatment was given twice a day, intraperitoneally, for 21 days. The @entity11 exhibited low @entity70586 values (before treatment), compared to normal brain, indicating the presence of high cell density @entity5 . Following GCV treatment, a regional increase in @entity70586 was observed as early as day 4 of the treatment, even though the @entity5 volume was still increasing. These observations suggested evolution of local necroses which were confirmed by histology. In a group of five @entity5 bearing @entity35 , retrovirus-producing packaging cell injections were given intratumorally to mimic clinically relevant gene therapy. In these cases, only small and short-lasting @entity70586 elevations were found following GCV treatment without an effect on the overall @entity5 growth and outcome. Our results show that quantitative magnetic resonance imaging including @entity70586 , is superior to robust volume measurements in predicting an early response to retrovirus-mediated gene therapy in vivo.
