Cholinergic cells in the tegmentum send branching projections to the inferior colliculus and the XXXX .

@entity717 @entity1877 @entity6324
The pontomesencephalic tegmentum (PMT) provides cholinergic input to the inferior colliculus (IC) and the @entity6324 ( @entity6324 ). PMT cells are often characterized as projecting to more than one target. The purpose of this study was to determine whether individual PMT cholinergic cells, (1) innervate the auditory pathways bilaterally via collateral projections to left and right auditory thalamus; or, (2) innervate multiple levels of the auditory pathways via collateral projections to the auditory thalamus and inferior colliculus. We used multiple retrograde tracers to identify individual PMT cells that project to more than one target. We combined the retrograde tracer studies with immunohistochemistry for @entity1877 acetyltransferase to determine whether the projecting cells were cholinergic. We found that individual PMT cells send branching axonal projections to two or more auditory targets in the midbrain and thalamus. The collateral projection pattern that we observed most frequently was to the @entity717 . Cells projecting to both MGs were somewhat less common, followed by cells projecting to the contralateral IC and @entity717 . Both cholinergic and non-cholinergic cells contribute to each of these projection patterns. Less often, we found cells that project to one IC and both MGs; there was no evidence for non-cholinergic cells in this projection pattern. It is likely that collateral projections from PMT cells could have coordinated effects bilaterally and at multiple levels of the ascending auditory pathways.
