Empty pericarp2 encodes a negative regulator of the @entity1457 and is required for XXXX embryogenesis.

@entity1457 @entity851 @entity2829 @entity71056
The @entity1457 ( @entity1457 ) is an evolutionarily conserved molecular/biochemical reaction to thermal stress that is essential to the survival of eukaryotic organisms. Recessive Mutator transposon mutations at the @entity2829 empty pericarp2 ( @entity71056 ) locus led to dramatically increased expression of @entity1457 genes, retarded embryo development, and early-stage abortion of embryogenesis. The developmental timing of @entity71056 mutant embryo lethality was correlated with the initial competence of @entity2829 kernels to invoke the @entity1457 . Cloning and sequence analyses revealed that the @entity71056 gene encoded a predicted protein with high similarity to HEAT SHOCK BINDING PROTEIN1, which was first described in animals as a negative regulator of the @entity1457 . @entity71056 is a loss-of-function mutation of an @entity1457 -negative regulator in plants. Despite the recessive @entity71056 phenotype, steady state levels of @entity71056 transcripts were abundant in mutant kernels, and the predicted coding region was unaffected. These expression data suggest that @entity71056 transcription is feedback regulated, whereas S1 nuclease mapping suggests that @entity71056 mutant transcripts are 5' truncated and nontranslatable. In support of this model, immunoblot assays revealed that @entity71056 protein did not accumulate in mutant kernels. These data support a model whereby an unattenuated @entity1457 results in the early abortion of @entity71056 mutant embryos. Furthermore, the @entity851 of @entity71056 mutant kernels before the @entity1457 suggests an additional role for @entity71056 during embryo development distinct from the @entity1457 .
