Selection of lineage-restricted cell lines immortalized at different stages of hematopoietic differentiation from the XXXX cell line 32D.

@entity2304 @entity14190 @entity19 @entity24778 @entity5 @entity3019 @entity18446 @entity4778 @entity16748
@entity14190 ( @entity14190 ), @entity2304 ( @entity2304 ) and granulocyte colony-stimulating factor- ( @entity24778 ) dependent cell lines have been derived from the @entity19 hematopoietic cell line 32D with a selection strategy involving the culture of the cells in FBS-deprived medium supplemented only with pure recombinant @entity14190 , @entity2304 , or @entity24778 . The cells retain the diploid karyotype of the original 32D clone, do not grow in the absence of exogenous growth factor, and do not induce @entity5 when injected into syngeneic recipients. The morphology of the @entity14190 -dependent cell lines (32D Epo1, -2, and -3) was heterogeneous and evolved with passage. The percent of differentiated cells also was a function of the cell line investigated. @entity16748 -positive cells ranged from 1-2% (32D Epo3) to 50-60% (32D Epo1). These erythroid cells expressed @entity4778 and/or globin mRNA but not @entity18446 . The @entity2304 - and @entity24778 -dependent cell lines had predominantly the morphology of undifferentiated myeloblasts or metamyelocytes, respectively. The @entity2304 -dependent cell lines were sensitive to either @entity2304 or @entity3019 ( @entity3019 ) but did not respond to @entity24778 . The @entity24778 -dependent cell lines grew to a limited extent in @entity3019 but did not respond to @entity2304 . These results indicate that the cell line 32D, originally described as predominantly a basophil/mast cell line, has retained the capacity to give rise to cells which proliferate and differentiate in response to @entity14190 , @entity2304 , and/or @entity24778 . These cells represent the first nontransformed cell lines which can be maintained in growth factors other than @entity3019 and which differentiate in the presence of physiologic signals. As such, they may represent a model to study the molecular mechanisms underlying the process of hematopoietic differentiation, as well as sensitive targets for bioassays of specific growth factors.
