Glucocorticoid treatment of ovariectomized XXXX affects mineral density, structure, and mechanical properties of cancellous bone.

@entity1667 @entity1 @entity742 @entity170 @entity134 @entity2331 @entity367 @entity6092
Thus far, orthopedic research lacks a suitable animal model of @entity170 . In OVX @entity742 , 6 months of @entity134 exposure reduced bone density and mechanical competence. Bone properties and bone formation did not recover for another 6 months. Therefore, @entity134 -treated OVX @entity742 may serve as a large animal model for @entity6092 bone. INTRODUCTION: The purpose of this study was to explore the effects of glucocorticoid treatment on cancellous bone density, microarchitecture, biomechanics, and formation of new bone. MATERIALS AND METHODS: Sixteen ovariectomized merino @entity742 received either a 6-month glucocorticoid treatment ( @entity367 ; 0.45 mg/kg @entity1667 ) or were left untreated (control). Cancellous bone biopsy specimens from the tibia were harvested 6 months after ovariectomy. After 12 months, the animals were killed, and biopsy specimens were obtained from the contralateral tibia and the lumbar spine. All biopsy specimens were scanned for apparent bone mineral density by peripheral quantitative computed tomography (pQCT) and tested mechanically in uniaxial compression. Three-dimensional bone reconstructions were obtained by microcomputed tomography. Formation of new bone was analyzed using histologies of the femoral condyles. RESULTS: After 6 months, mineral density (-19%) and mechanical competence (-45%) were reduced by glucocorticoid treatment (p < 0.1). BV/TV (-21%; p < 0.01) and trabecular thickness (-20%; p = 0.01) declined, whereas BS/BV increased (24%; p = 0.01). After 12 months, mineral density (-33%) and mechanical properties (-55%) were reduced even more profoundly (p < 0.05). Also, the structural parameters (BS/BV and Tb.Th.) still seemed to be affected by glucocorticoid treatment (p < 0.05). New bone formation, assessed by measurement of osteoid surface, was markedly reduced (-63%, p < 0.1) by glucocorticoid treatment. The differences between groups were generally more pronounced at the tibia and the femur than at the spine. CONCLUSION: The effects of short-term high-dose @entity134 administration on bone mineral in this animal model were comparable with those observed in @entity1 after long-term corticoid treatment. Reduction in bone quality and bone formation rate persisted after the cessation of @entity134 administration. Glucocorticoid treatment of ovariectomized @entity742 may therefore serve as a large animal model for @entity134 -induced @entity2331 .
