Pathological changes in acute experimental toxoplasmosis with @entity3204 strains obtained from XXXX cases of congenital disease.

@entity1 @entity19 @entity281 @entity13570 @entity3251 @entity3204
UNASSIGNED: There is a lack of studies using @entity3204 strains isolated from @entity1 @entity1 . Here, we present a pathological study of three strains obtained from @entity1 cases of @entity3251 in Brazil using inbred @entity19 after @entity281 with 10 tissue cysts. Multiplex-nested PCR-RFLP of eleven loci revealed atypical genotypes commonly found in Brazil: toxodb #8 for TgCTBr5 and TgCTBr16 strains and toxodb #11 for the TgCTBr9 strain. BALB/c and C57BL/6 @entity19 were evaluated for survival and histological changes during the acute phase of the disease. All @entity19 inoculated with the non-virulent TgCTBR5 strain survived after 30 days, although irreversible tissue damage was found. In contrast, no @entity19 were resistant to infection with the highly virulent TgCTBR9 strain. The TgCTBr16 strain resulted in 80% survival in @entity19 . However, this strain presented @entity281 , especially by the oral route of infection. Despite being identified with the same genotype, TgCTBr5 and TgCTBr16 strains showed biological differences. Histopathologic analysis revealed liver and lungs to be the most affected organs, and the pattern of tissue injury was similar to that found in @entity19 inoculated perorally with strains belonging to clonal genotypes. However, there was a variation in the intensity of ileum lesions according to @entity3204 and @entity19 lineage. C57BL/6 @entity19 showed higher susceptibility than BALB/c for histological lesions. Taken together, these results revealed that the pathogenesis of @entity3204 strains belonging to atypical genotypes can induce similar tissue damage to those from clonal genotypes, although intrinsic aspects of the strains seem critical to the induction of @entity13570 in the infected host.
