Molecular mechanisms of apoptosis induced by XXXX .

@entity5 @entity29654 @entity14165 @entity4675 @entity30615
@entity29654 ( @entity29654 ; ECyd), a @entity14165 analog, has a potent cytotoxic activity against @entity5 cells. We have investigated the @entity5 induced by ECyd, focusing on its molecular mechanisms. In ECyd-treated cells, @entity30615 is activated and involved in @entity4675 ( @entity4675 ) phosphorylation, followed by induction of mitochondria-dependent apoptosis. The mechanism of @entity4675 phophorylation by @entity30615 was unknown. To investigate the mechanism, we performed the identification of @entity30615 -binding partners by proteomic approach using co-immunoprecipitation and mass spectrometry. We found that @entity30615 was associated with a protein (we named it Protein-190). At the same time, we observed that Protein-190 was amply phosphorylated. Furthermore, the participation of Protein-190 in the ECyd-induced apoptosis was supported by a knockdown experiment using small interfering RNA (siRNA). Thus, the number of ECyd-induced apoptotic cells was drastically decreased when Protein-190 was knocked-down. These results indicated Protein-190 as a regulator in apoptosis, and provide the possibility for a new clinical target in @entity5 chemotherapy.
