Association of autophagy related gene polymorphisms with @entity32 in adult XXXX .

@entity1 @entity32 @entity70828 @entity70826 @entity565 @entity70827 @entity10614 @entity10615 @entity70830 @entity70829 @entity687
Background/Aims: Role of autophagy in neutrophil function and the association of autophagy and autophagy related (ATG) gene polymorphisms with @entity565 susceptibility were suggested. In this study, we investigated the genetic association of @entity10614 and @entity10615 polymorphisms with @entity565 risk, severity and @entity32 . Methods: We recruited 408 @entity565 @entity1 and 201 healthy controls. Sputum neutrophil counts were determined by H_E staining. Serum @entity687 ( @entity687 ) levels were measured by enzyme-linked immunosorbent assay (ELISA). Genetic polymorphisms of @entity10614 ( @entity70826 , @entity70827 , and @entity70828 ) and @entity10615 ( @entity70829 and @entity70830 ) were genotyped. The functional activities of @entity10614 @entity70826 and @entity70827 variants were investigated by luciferase reporter assays. Results: No associations of @entity10614 and @entity10615 polymorphisms with @entity565 susceptibility and severity were found. @entity10614 @entity70826 and @entity70827 were in complete linkage disequilibrium. In the @entity565 group, GA/AA genotypes at @entity10614 @entity70827 were associated with higher neutrophil counts in sputum (p < 0.05); CC/TT genotype at @entity10614 @entity70828 associated with lower FEV1% predicted value (p < 0.05). DNA fragments containing @entity10614 -769T and -335G alleles had higher promoter activities compared to those with -769C and -335A in both @entity1 airway epithelial cells (A549, p < 0.01) and @entity1 mast cell (HMC-1, p < 0.001). GG and CC genotype at @entity10615 @entity70829 and @entity70830 were significantly associated with high serum levels of @entity687 (p < 0.05 for both variants). Conclusions: Genetic polymorphisms of @entity10614 and @entity10615 could contribute to @entity32 in the pathogenesis of adult @entity565 .
