[Research of the influence and mechanism about @entity2449 on the proliferation of differentiated XXXX cells].

@entity1204 @entity1 @entity470 @entity5 @entity3962 @entity20830 @entity1366 @entity2449
OBJECTIVE: To investigate the effects of @entity2449 on cell proliferation in differentiation degree of @entity1204 cells and related mechanisms. METHODS: The @entity1204 cell lines Ishikawa and @entity1 were used. Cell proliferation was assessed after exposure to @entity2449 with or without @entity470 ( @entity470 ) inhibitor @entity20830 by cell counting kit-8 (CCK-8) method. @entity470 mRNA was determined by reverse transcription (RT)-PCR. The expression of phosphorylation @entity470 (p- @entity470 ) and total @entity470 (t- @entity470 ) and phosphorylation @entity3962 (p- @entity1366 ) and total @entity1366 (t- @entity1366 ) were examined by western blot. RESULTS: (1) CCK-8 experiment showed that @entity2449 could inhibit the proliferation of @entity1204 cells in a time-dependent manner and a dose-dependent manner (P < 0.05), but the inhibition of well differentiated cell line Ishikawa was lower than that in poorly differentiated cells AN3CA (P < 0.05). @entity20830 also could inhibit the proliferation of @entity1204 cells in a time-dependent manner and in a dose-dependent manner (P < 0.05), but the inhibition rate of well differentiated cell line Ishikawa was higher than that in poorly differentiated cells AN3CA (P < 0.05). @entity2449 + @entity20830 also could inhibit the proliferation of @entity1204 cells in a time-dependent manner and in a dose-dependent manner (P < 0.05), and the inhibition of combined with @entity2449 and @entity20830 was stronger than that with a single application of drugs, but the inhibition rate of Ishikawa was higher than that in AN3CA (P < 0.05). (2) RT-PCR method showed that different concentrations of @entity2449 (0.01, 0.1, 1, 5, 10 mmol/L, respectively) for 24 hours, the expression level of @entity470 mRNA in Ishikawa cells were respectively 0.74    0.03, 0.61    0.04, 0.46    0.03, 0.31    0.03 and 0.23    0.03, the expression level of @entity470 mRNA in @entity1 cells were respectively 0.79    0.20, 0.61    0.03, 0.50    0.05, 0.32    0.03 and 0.26    0.04, the inhibition effect showed a significant concentration-dependent manner (all P < 0.01). (3) Western blot method displayed that the effect of @entity2449 treated respectively 2, 4, 6 or 8 hours, there were not significant difference in the expression levels of t- @entity470 protein and t- @entity1366 between Ishikawa and @entity1 cells (all P > 0.05). But the expression levels of p- @entity470 and p- @entity1366 protein were significantly lower between two groups (P < 0.01), which showed a time-dependent manner (P < 0.01). CONCLUSION: @entity2449 could inhibit the proliferation of @entity1204 cells, the inhibition is associated with the differentiation degree of @entity5 cells. @entity2449 could enhance the @entity470 signaling pathway inhibitor @entity20830 inhibition of @entity1204 cells, which may inhibit @entity470 expression of phosphorylated proteins to inhibit the phosphorylation of @entity1366 proteins and then inhibit proliferation of @entity1204 cells.
