Steady state expression of cell-cycle regulatory genes in XXXX cell lines.

@entity263 @entity2534 @entity5438 @entity9288 @entity8985 @entity876 @entity2533 @entity994
The @entity2533 ( @entity2533 ) signal transduction system has been reported to play a role in prostate tumorigenesis and the regulation of cell cycle-related gene expression including the cyclins, cyclin dependent kinases (Cdks), and Cdk inhibitors. The objective of this investigation was to examine the expression of @entity2534 and five cell cycle-related genes- @entity8985 , @entity9288 , @entity994 (( @entity994 ), @entity5438 , and cyclin E-in three @entity263 cell lines and normal prostate by quantitative reverse transcriptase (RT)/polymerase chain reaction (PCR). The expression of the @entity2534 was reduced by 5.5- and 2.2-fold in the LNCaP and DU145 cells, respectively, compared with normal prostate tissue. A similar decrease was observed for the @entity2533 receptor I transcript in the LNCaP cells. In addition, 20-fold less of the @entity2533 inducible @entity9288 (inkb) transcript was produced by the LNCaP and DU145 cell lines compared with the PC-3 cell line. The @entity994 (( @entity994 ) transcript, although present, was only 6% of normal in the DU145 and PC-3 cell lines. Furthermore, the steady state levels of @entity994 (( @entity994 ) mRNA significantly increased within 15 minutes after the addition of exogenous @entity2533 to PC-3 cells. Likewise, addition of @entity2533 antibodies to the PC-3 cells significantly reduced @entity994 (( @entity994 ) transcript levels to less than 2% of normal. This suggests that @entity994 (( @entity994 ) expression in PC-3 cells is related not only to @entity876 induction but may function through alternative pathways including @entity2533 . We conclude that the expression of specific cell cycle-related genes may be entirely or partially regulated by alterations in @entity2533 pathway and may play a @entity263 development.
