XXXX ChIP-sequencing of subcutaneous fat reveals modulation of inflammatory pathways.

@entity1 @entity32 @entity41145 @entity4738 @entity9170 @entity28 @entity619 @entity3470
OBJECTIVE: To identify @entity4738 ( @entity4738 )-associated chromatin sequences and target genes in primary @entity1 abdominal subcutaneous fat. METHODS: @entity4738 chromatin immunoprecipitation (ChIP)-sequencing (seq) methodology in subcutaneous @entity1 adipocytes treated ex vivo with @entity619 ( @entity9170 ) was optimized to identify genome-wide @entity9170 -dependent @entity4738 -binding regions (GBRs). Gene expression analyses were performed in parallel        @entity9170 treatment. RESULTS: Fat was obtained from four female surgical @entity1 without @entity28 with a median age of 50.5 years. ChIP-seq analysis revealed 219 @entity9170 -associated GBRs. Of these, 136 GBRs were located within 100 kb of the transcriptional start site and associated with 123 genes. Combining these data with @entity9170 -induced gene expression, 70 of the 123 putative direct target genes were significantly up- or downregulated following 4 hours of @entity9170 treatment. Gene expression analysis demonstrated that the top 10 pathways reflected regulation of cellular metabolism and @entity32 . @entity41145 , an inhibitor of @entity3470 , was identified as a potential direct @entity4738 target gene. CONCLUSIONS: This is the first report of genome-wide GR ChIP-seq and gene expression analysis in @entity1 fat. The results implicate regulation of key @entity4738 target genes that are involved in dampening @entity32 and promoting cellular metabolism.
