The anti- XXXX effects of a tuna peptide on 3T3-L1  adipocytes are mediated by the inhibition of the expression of lipogenic and adipogenic genes and by the activation of the Wnt/b-catenin signaling pathway.

@entity70613 @entity6913 @entity3208 @entity28 @entity16509 @entity412 @entity2975 @entity413 @entity214
UNASSIGNED: The differentiation of 3T3-L1 cells into adipocytes involves the activation of an organized system of @entity28 -related genes, of which those encoding CCAAT/enhancer  -binding proteins  (C/EBPs) and the @entity70613 protein may play integral roles. In a previous study of ours, we found that a specific peptide found in tuna (sequence D  -I  -V  -D  -K  -I  -E  -I; termed TP  -D) inhibited 3T3  -L1 cell differentiation. In the present study, we observed that the expression of expression of C/EBPs and @entity70613 was associated with @entity28 . The initial step of 3T3  -L1 cell differentiation involved the upregulation of @entity16509  -a expression, which in turn activated various subfactors. An upstream effector of @entity6913  ( @entity6913 ) inhibited @entity70613 expression in 3T3  -L1 adipocytes. In a previous study of ours, we sequenced the tuna peptide via @entity3208 - @entity2975 gel electrophoresis  ( @entity214  -PAGE) and quadrupole time-of-flight mass spectrometry  (Q-TOF MS/MS) and confirmed the anti  - @entity28 effects thereof in 3T3  -L1 adipocytes. In the present study, we demonstrate that TP  -D inhibits @entity16509 and promotes @entity70613 mRNA expression, thus activating the Wnt pathway. The inhibition of lipid accumulation was measured using a @entity413 and @entity412  (TG) assay. Our results confirmed that TP  -D altered the expression levels of @entity16509  -related genes in a dose  -dependent manner and activated the Wnt signaling pathway. In addition, we confirmed that total adiponectin and high  -molecular weight  (HMW) adiponectin levels were reduced by treatment with TP  -D. These data indicate that TP  -D inhibits adipocyte differentiation through the inhibition of @entity16509 genes and the subsequent activation of the Wnt/b-catenin signaling pathway.
