Transcriptional profiles of laccase genes in the brown rot fungus XXXX MAD-R-698.

@entity1568 @entity28233 @entity3787 @entity920 @entity71445 @entity71446 @entity1068 @entity413
One of the laccase isoforms in the brown rot fungus @entity71445 is thought to contribute to the production of @entity3787 radicals, which play an important role in lignocellulose degradation. However, the presence of at least two laccase isoforms in this fungus makes it difficult to understand the details of this mechanism. In this study, we systematically investigated the transcriptional patterns of two laccase genes, Pplcc1 and Pplcc2, by quantitative PCR (qPCR) to better understand the mechanism. The qPCR results showed that neither of the two genes was expressed constitutively throughout growth in liquid culture or during the degradation of a woody substrate. Transcription of Pplcc1 was upregulated under @entity1568 depletion and in response to a high concentration of @entity920 in liquid culture, and during the initial colonization of intact aspen wafer. However, it was subject to catabolite repression by a high concentration of @entity413 . Transcription of Pplcc2 was upregulated by stresses caused by @entity28233 , @entity71446 , and @entity1068 , and under osmotic stress in liquid culture. However, the transcription of Pplcc2 was downregulated upon contact with the woody substrate in solid culture. These results indicate that Pplcc1 and Pplcc2 are differentially regulated in liquid and solid cultures. Pplcc1 seems to play the major role in producing @entity3787 radicals and Pplcc2 in the stress response during the degradation of a woody substrate.
