[A cell kinetic study of @entity1 central nervous system tumors with XXXX ( @entity1603 )].

@entity1 @entity1603 @entity5513 @entity424 @entity7432 @entity5 @entity3162 @entity11 @entity1653 @entity982 @entity816 @entity463 @entity3 @entity977 @entity2014 @entity1068 @entity2946
Sixty-six @entity1 with various types of central nervous system (CNS) @entity5 were given 150-200 mg/m2 of @entity1603 ( @entity1603 ) intravenously at the start of surgery, in order to label DNA synthesizing cells in the @entity5 with @entity1603 . The excised @entity5 specimens were fixed in chilled 70% @entity1068 for at least 12 hours, embedded in @entity982 , and cut into 6-8 mu thick sections. The tissue sections were deparaffinized, denatured with @entity7432 , and stained by indirect peroxidase method using anti- @entity1603 monoclonal antibody as the first antibody. The percentage of labeled cells among total cells ( @entity3162 : labeling index) was calculated in each @entity5 specimen. The average of LIs was over 10% in @entity463 multiforme, @entity2946 , and @entity3 . On the other hand, the LIs were very low, i.e. less than 1% in most cases of @entity424 , @entity11 , and moderately @entity816 . In highly @entity816 , the average of LIs resulted in 7.4%. The LIs of slow-growing @entity5 , such as @entity977 , @entity1653 , @entity5513 , were less than 1%, except in @entity2014 and a few cases of @entity1653 . The LIs obtained thus seem to correlate with the clinical behavior of each type of @entity5 . However, there was still a magnitude of differences in LIs between the @entity5 which were histopathologically alike. These results indicate that the @entity3162 obtained by the immunohistochemical method using anti- @entity1603 monoclonal antibody may supplement the current histopathological diagnosis in characterizing as well as estimating the biological malignancy of individual CNS @entity5 .
