Involvement of Opioid Receptors in the Lipid Rescue of XXXX -Induced @entity1370 .

@entity4613 @entity1510 @entity1341 @entity1370 @entity35 @entity19244 @entity1445
BACKGROUND: Lipid emulsion (LE) has been successfully used for resuscitation of local anesthetic @entity1370 caused by @entity1341 @entity1445 . Opioid receptors have been shown to play a key role in cardio protection. We explored whether this rescue action of LE is mediated through opioid receptors. METHODS: Asystole was induced by @entity1341 (10 mg/kg over 20 seconds, IV) in young male @entity35 , and resuscitation with LE (intralipid 20%; 5 mL/kg bolus and 0.5 mL/kg/min maintenance) was started immediately. The @entity35 were pretreated 2 minutes before inducing asystole with nonselective opioid receptor antagonists such as @entity1510 and @entity19244 , as well as highly selective opioid receptor antagonists for subtype k,   , and    or @entity4613 buffer solution as a control. Heart rates and ejection fractions were measured using echocardiography. RESULTS: LE rescue of @entity1341 @entity1370 was prevented by high-dose (1 mg/kg) @entity1510 but not by lower doses of @entity1510 (1, 5, and 10   g/kg), by @entity19244 (which does not cross the blood-brain barrier), and by a selective   - and k-opioid receptor antagonists at a higher (10 mg/kg) dose. Successful LE rescue was not affected by highly selective   -opioid receptor antagonists.   -Opioid receptor antagonist (10 mg/kg) pretreatment also resulted in reduced phosphorylation level of cardiac glycogen synthase kinase-3b in @entity35 that were not resuscitated by LE compared with control. CONCLUSIONS: Our data highlight the involvement of peripheral   - and k-opioid receptors in the rescue action of LE.
