Flow cytometric analysis of XXXX bone marrow. II. Normal B lymphocyte development.

@entity1 @entity10730 @entity10911 @entity1075 @entity2225 @entity2282 @entity2280 @entity10317
A panel of B lymphoid-reactive monoclonal antibodies was used to analyze the phenotypic changes that accompany B lymphocyte development in normal @entity1 bone marrow. The B lymphoid cells were identified using light scattering and the expression of @entity1075 on a flow cytometer. Quantitative three-color immunofluorescence was then used to correlate other cell surface antigens on these cells identified as B lymphoid in normal marrow. @entity2282 and @entity2225 identified almost exclusive populations and provided a convenient means of discriminating between the less and more mature B lineage cells. The @entity2282 + cells could be further subdivided using @entity2280 . The population of @entity1075 +, @entity2282 +, @entity2280 + cells comprised only 0.6% of marrow cells, but these contained the majority of @entity10730 ( @entity10730 +) cells. In the assessment of class II antigens, HLA-DR was expressed on all B lineage cells whereas HLA-DP preceded HLA-DQ in appearance during the developmental process. Among the later antigens expressed on B lineage cells, cell surface IgM, @entity2225 , and HLA-DQ were expressed at essentially the same time. Cell surface @entity2282 was lost at the time when @entity10911 and @entity10317 were acquired on the cell surface. These data illustrate that multiparameter flow cytometry can be used to define a continuous progression of stages of B lymphocyte development based on cell surface antigen expression even though these cells represent a minor fraction of normal marrow cells.
