@entity3978 oxidase activity in subcellular fractions of XXXX contaminated by @entity2555 .

@entity3978 @entity2555 @entity18959 @entity2556 @entity11966
Understanding enzyme responses to contamination with persistent organic pollutants (POPs) is a key step in the elucidation of POP metabolic mechanisms in plants. However, there is little information available on enzyme activity in subcellular fractions of POP-contaminated plants. To our knowledge, this is the first study to investigate the activities of @entity3978 oxidase (PPO) in cell fractions of plants under contamination stress from @entity2555 ( @entity2556 ) using a greenhouse batch technique. Three parameters, E(cell), E(cell-n), and P(cell), denoting the amount of PPO activity, cell fraction content-normalized PPO activity, and proportion of PPO activity in each cell fraction, respectively, were used in this study. Contamination with @entity11966 , as a representative PAH, at a relatively high level (>0.23 mg L     ) in culture solution generally stimulated PPO activity in @entity18959 ( @entity18959 .) roots and shoots and their cellular fractions. The amount and distribution proportion of PPO activity in each cell fraction of @entity11966 -contaminated roots and shoots were (in descending order): cell solution > > cell wall > cell organelles. Cell solution was the dominant storage domain of PPO activity and contributed 84.0 and 82.8% of PPO activity in roots and shoots, respectively. The cell wall had the highest density of PPO activity in roots and shoots, based on the highest cell fraction content normalized PPO activity in this cell fraction. Our results provide new information on enzyme responses in plant intracellular fractions to xenobiotic POPs and fundamental information on within-plant POP metabolic mechanisms.
