Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating XXXX .

@entity1 @entity6 @entity19 @entity519 @entity4537 @entity446 @entity466
Stage-specific embryonic antigen-3 (SSEA-3)-positive multipotent mesenchymal cells (multilineage differentiating stress-enduring [Muse] cells) were isolated from cultured @entity1 adipose tissue-derived stem/stromal cells (hASCs) and characterized, and their therapeutic potential for treating @entity4537 was evaluated. Cultured hASCs were separated using magnetic-activated cell sorting into positive and negative fractions, a SSEA-3(+) cell-enriched fraction (Muse-rich) and the remaining fraction (Muse-poor). Muse-rich hASCs showed upregulated and downregulated pluripotency and cell proliferation genes, respectively, compared with Muse-poor hASCs. These cells also released higher amounts of certain growth factors, particularly under @entity519 conditions, compared with Muse-poor cells. Skin ulcers were generated in @entity466 ( @entity466 ) @entity19 with type 1 @entity6 , which showed delayed wound @entity446 compared with nondiabetic @entity466 @entity19 . Treatment with Muse-rich cells significantly accelerated wound @entity446 compared with treatment with Muse-poor cells. Transplanted cells were integrated into the regenerated dermis as vascular endothelial cells and other cells. However, they were not detected in the surrounding intact regions. Thus, the selected population of ASCs has greater therapeutic effects to accelerate impaired wound @entity446 associated with type 1 @entity6 . These cells can be achieved in large amounts with minimal morbidity and could be a practical tool for a variety of stem cell-depleted or ischemic conditions of various organs and tissues.
