XXXX transporter splice variant expression in an enriched pyramidal cell population in @entity161 .

@entity3264 @entity20539 @entity9320 @entity161 @entity3689 @entity6485
UNASSIGNED: Dysregulation of the @entity3264 transporters @entity6485 and @entity9320 and their isoforms have been implicated in @entity161 . @entity6485 and @entity9320 expression has been studied in different brain regions but the prevalence of astrocytic @entity3264 transporter expression masks the more subtle changes in excitatory @entity3689 transporters (EAATs) isoforms in neurons in the cortex. Using laser capture microdissection, pyramidal neurons were cut from the anterior cingulate cortex of postmortem @entity161 (n=20) and control (n=20) subjects. The messenger RNA (mRNA) levels of @entity6485 , @entity9320 and the splice variants @entity6485 exon9skipping, @entity9320 exon9skipping and EAAT2b were analyzed by real time PCR (RT-PCR) in an enriched population of neurons. Region-level expression of these transcripts was measured in postmortem @entity161 (n=25) and controls (n=25). The relationship between selected EAAT polymorphisms and EAAT splice variant expression was also explored. Anterior cingulate cortex pyramidal cell expression of EAAT2b mRNA was increased (P<0.001; 67%) in @entity161 subjects compared with controls. There was no significant change in other EAAT variants. @entity9320 exon9skipping mRNA was increased (P<0.05; 38%) at region level in the anterior cingulate cortex with no significant change in other EAAT variants at region level. @entity9320 single-nucleotide polymorphisms were significantly associated with changes in @entity9320 isoform expression. @entity20539 -treated animals, acting as controls for possible antipsychotic effects, did not have significantly altered neuronal EAAT2b mRNA levels. The novel finding that EAAT2b levels are increased in populations of anterior cingulate cortex pyramidal cells further demonstrates a role for neuronal @entity3264 transporter splice variant expression in @entity161 .
