Development of RAPD- XXXX markers for Lonicera japonica (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning.

@entity8470 @entity30
Genetic diversity within a species is a common feature, which plays a vital role in its survival and adaptability, and is important for the identification and authentication of a species. Lonicera japonica is a traditionally used medicinal plant, which have been recently genetically characterized by an improved ran- dom amplified polymorphic DNA (RAPD) analysis. In this study, the molecular markers on the basis of these RAPD fragments have been developed to identify specific @entity8470 variety. The DNAs were extracted from fresh young leaves of different samples of @entity8470 collected from Shenzhen, Yichang, Leshan, Emei and Loudi, China. The DNA materials were amplified using improved RAPD PCR. Different RAPD bands were excised, cloned and developed for stable @entity30 ( @entity30 ) markers with differ- ent species. Two @entity30 markers, JYH3-3 and JYH4-3, have been successfully cloned from improved.RAPD fragments. The @entity30 marker JYH3-3 was found specific for all of the @entity8470 samples collected from the different regions, and another marker JYH 4-3 was strictly specific to the Shenzhen sample from Guangdong province, which is geographically distant from Hubei, Sichuan and Hunan Provinces (source of other @entity8470 samples). The marker JYH3-3 was found as specific molecular marker for the identification of @entity8470 , while JYH4-3 was found as molecular marker strictly specific for the Shenzhen sample. The developed @entity30 markers might serve as more specific molecular markers for @entity8470 variety authentication. The combination of improved RAPD analysis and @entity30 marker development have resulted useful tools to study the genetic variety of any organism, which we have successfully applied here in @entity8470 .
