Isolation and characterization of side population cells from the @entity1 XXXX cell line SK-OV-3.

@entity43 @entity1 @entity5 @entity900 @entity1759 @entity855
UNASSIGNED: @entity43 ( @entity43 ) is the most @entity5 due to its high recurrence rate following initial treatment. Previous studies have indicated that @entity5 stem cells (CSCs) may be a potential cause underlying the high proportion of recurrence. Side population (SP) cells isolated from @entity5 cell lines have been shown to exhibit characteristics associated with CSCs, but studies on SP cells in @entity1 ovarian SK-OV-3 cell line are limited. In the present study, the SP cell fraction (4.83% of the total cell population) was isolated using flow cytometry, and analyzed by immunocytochemical analysis and reverse transcription-quantitative polymerase chain reaction. The results showed that SP cells exhibited a high mean fluorescence intensity for @entity1759 , a CSC marker, in addition to elevated expression of the CSCs-associated genes, @entity855 -binding cassette sub-family G member 2 and Nestin. These findings indicated the stem cell-like features of the SP cells. Furthermore, a colony formation test showed that the isolated SP cells possessed a marked capacity for self-regeneration and proliferation. In addition, a cell cycle assay involving @entity900 indicated that the SP cells were strongly resistant to chemotherapy. In conclusion, the present results suggested that SP cells isolated from the SK-OV-3 cell line exhibited properties typically associated with CSCs. Therefore, the isolated SP cells may be used to provide novel insight into potential therapies against @entity43 .
