Remote Ischemic Preconditioning Improves the Viability of Donor Lipoaspirate during XXXX Fat Transfer.

@entity82 @entity63 @entity19 @entity10051 @entity130
BACKGROUND: Variable results associated with fat grafting have been attributed to local @entity130 , inconsistencies in transfer, and @entity63 before the development of recipient circulation. Remote @entity63 preconditioning is an inexpensive noninvasive technique that has been used in animal models and multicenter clinical trials to protect organ systems. In this work, the authors describe a novel animal model for analyzing the efficacy of fat grafting, and investigate the effect of remote @entity63 preconditioning on volume retention. METHODS: Subcutaneous adipose tissue samples from green fluorescent protein/luciferase-expressing FVB @entity19 were obtained with or without pretreatment with a temporary hind-limb tourniquet. The samples were injected into the dorsal skin folds of wild-type FVB @entity19 . The viability of the transferred tissue was examined over a 28-day period with quantitative bioluminescence after @entity10051 injection. Transferred tissue was also explanted for histologic analysis. RESULTS: The remote @entity63 preconditioning group had significantly increased bioluminescence at days 0, 1, and 28. Histologic analysis at day 28 confirmed the presence of vascularized adipose in both groups. However, significant amounts of @entity82 were found in the control group, whereas substantially less was found in the remote @entity63 preconditioning group. The remote @entity63 preconditioning group retained a substantially greater amount of green fluorescent protein, suggesting increased survival of donor adipocytes. CONCLUSIONS: In this work, the authors describe a novel animal model for quantitative evaluation of fat grafting using in vivo bioluminescence of adipocytes from luciferase-expressing @entity19 . The authors also demonstrate that remote @entity63 preconditioning increases the viability of fat transfer and decreases @entity82 .
