Lens epithelial cell death after XXXX surgery.

@entity1 @entity11579 @entity231 @entity19 @entity856
PURPOSE: To determine whether lens epithelial cells (LECs) undergo apoptosis during healing after @entity231 surgery to further characterize the healing process of the postoperative lens capsule. SETTING: Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan. METHODS: Apoptotic cells were detected in @entity1 postoperative lens capsules using the terminal deoxynucleotidyl transferase deoxy- @entity856 nick-end labeling (TUNEL) method. The effect of exogenous @entity11579 ( @entity11579 ) on @entity19 LEC apoptosis was also examined in an organ-culture system. RESULTS: Three of 17 postoperative specimens contained TUNEL-positive cells. In 2 lens capsules obtained earlier than 10 days, many TUNEL-positive cells, presumably apoptotic LECs, were observed beneath the residual anterior capsule. In cell multilayers in capsule opacification extracted later than 10 days, a few TUNEL-positive cells were seen in 1 specimen; most cells remained negative. In @entity19 lenses organ-cultured with 1.0 ng/mL @entity11579 for 48 hours, TUNEL-positive cells were detected beneath the lens capsule. CONCLUSIONS: Lens epithelial cells undergo apoptosis during healing after @entity231 surgery, especially in the early phase. @entity11579 may be a factor inducing apoptosis in in vivo LECs.
