Phosphorylation of CaMKII in the XXXX dorsal raphe nucleus plays an important role in sleep-wake regulation.

@entity35 @entity14374 @entity11755 @entity15130 @entity141 @entity25
UNASSIGNED: @entity141 modulation in the dorsal raphe nucleus (DRN) plays an important role in sleep-wake regulation. Calmodulin-dependent kinase II (CaMKII) is an important signal-transducing molecule that is activated by @entity141 . The present study investigated the effects of intracellular @entity141 /CaMKII signaling in the DRN on sleep-wake states in @entity35 . Maximum and minimum CaMKII phosphorylation was detected at Zeitgeber time 21 (ZT 21; wakefulness state) and ZT 3 (sleep state), respectively, across the light-dark rhythm in the DRN in @entity35 . Six-hour sleep deprivation significantly reduced CaMKII phosphorylation in the DRN. Microinjection of the CAMKII activation inhibitor @entity14374 (5 or 10 nmol) into the DRN suppressed wakefulness and enhanced @entity11755 ( @entity11755 ) and non-REM sleep (NREMS). Application of a high dose of @entity14374 (10 nmol) increased @entity25 ( @entity25 ) time, @entity25 bouts, the mean duration of @entity25 , the percentage of @entity25 relative to total sleep, and delta power density during NREMS. Microinjection of @entity15130 (50 nmol) in the DRN increased CaMKII phosphorylation and decreased NREMS, @entity25 , and @entity11755 . @entity14374 abolished the inhibitory effects of @entity15130 on NREMS, @entity25 , and @entity11755 . These data indicate a novel wake-promoting and sleep-suppressing role for the @entity141 /CaMKII signaling pathway in DRN neurons. This article is protected by copyright. All rights reserved.
