Quantitative Determination of @entity68346 and XXXX in Different Extracts of Ligustrum Vulgare L. Leaves by a Validated HPTLC-Photodensitometry Method.

@entity32 @entity101 @entity6 @entity1044 @entity1042 @entity13918 @entity19185 @entity71538 @entity71539 @entity35839 @entity68346 @entity5189
INTRODUCTION: The genus Ligustrum (Oleaceae) is distributed in Europe and Asia (south China and Korea), where it is used to prevent @entity101 , sore throats, @entity32 and @entity6 . The main groups of compounds in extracts of Ligustrum vulgare are biologically active @entity68346 and @entity35839 . OBJECTIVES: The aim of the study was primarily the development and validation of a HPTLC-photodensitometry method for separation and determination of @entity68346 ( @entity71538 , @entity19185 ) and @entity35839 ( @entity71539 ) in different extracts prepared from leaves of L. vulgare. A secondary issue was the quantitative screening of @entity71538 , @entity19185 and @entity71539 in extracts from leaves collected at different stages of plant growth (from May to September). METHODS: A HPTLC-photodensitometry method was developed and validated for quantification of @entity19185 , @entity71538 and @entity71539 in plant extracts (aqueous and ethanolic extract, decoction, infusion). @entity13918 was used as the stationary phase and @entity1044 : @entity1042 : @entity5189 :water (80:25:1.5:4, v/v/v/v) as the mobile phase. RESULTS: The HPTLC-photodensitometry method developed for quantification of @entity71538 , @entity19185 and @entity71539 was specific, accurate and precise. The presence of @entity71538 was detected in aqueous extracts, whereas @entity19185 was present, in particular, in ethanolic extracts, decoctions and infusions. @entity71539 was detected in all the extracts prepared. The content of @entity68346 was variable from May to September, whereas the amount of @entity71539 increased in this term. CONCLUSION: The developed and validated HPTLC-photodensitometry method allowed performing fast screening of quantitative profiles of @entity71538 , @entity19185 and @entity71539 in preparations of privet leaves. Copyright    2015 John Wiley _ Sons, Ltd.
