Simultaneous measurement of multiple Th1 and Th2 serum cytokines in XXXX and correlation with disease severity.

@entity1 @entity1569 @entity6977 @entity379 @entity687 @entity688
BACKGROUND: Psoriatic plaques have been shown to contain increased levels of proinflammatory cytokines. Serum levels of interleukin (IL)-6, @entity6977 , @entity687 , and @entity379 have been reported elevated in psoriatic @entity1 . AIM: To evaluate serum cytokine profiles in @entity1569 @entity1 by improved enzyme-linked immunosorbent assay (ELISA) technique and to correlate these levels with disease severity. METHODS: We analyzed single serum samples from 10 @entity1 with active untreated @entity1569 , two @entity1 with active treated @entity1569 , and five healthy volunteers for major T helper type 1 and T helper type 2 cytokines using the LINCOplex ELISA multi-analyte detection system that permits simultaneous detection of multiple cytokines from a single sample. The disease severity, including erythema, induration, scale, and surface area, was assessed. RESULTS: @entity379 was markedly elevated in all sera from @entity1569 @entity1 , 33.8 +/- 1.3 pg/ml (mean +/- standard error) versus 8 +/- 1.5 pg/ml for normal controls (p < 0.01), and positively correlated with all indices of disease severity (Spearman r > 0.6). @entity687 was also increased in @entity1569 @entity1 (24.4 +/- 1.8 pg/ml) versus normal controls (3.6 +/- 1.2 pg/ml) (p < 0.05) and positively correlated with the degree of erythema (Spearman r > 0.6). Mean IL-12 levels were decreased in sera from @entity1569 @entity1 (8.5 +/- 1.2 pg/ml) compared with normal controls (42.2 +/- 5.3 pg/ml) (p < 0.01). Also, serum @entity688 levels were below detection levels in psoriatics compared with controls (6.4 +/- 1.3 pg/ml). CONCLUSIONS: This new ELISA system allowed rapid and reliable detection of numerous cytokines in single serum samples from @entity1 with @entity1569 . We observed that @entity379 and @entity687 cytokines were elevated in psoriatics and correlated with parameters of disease severity while @entity688 and IL-12 were decreased.
