Cell cycle checkpoint status in @entity1 XXXX cell lines: response to gamma radiation.

@entity1 @entity5438 @entity5 @entity2615 @entity876 @entity3620 @entity13525 @entity994
Knowledge of the function of the cell cycle checkpoints in @entity5 cells may be important to develop treatment strategies for @entity1 @entity5 . The protein @entity876 is an important factor that regulates cell cycle progression and apoptosis in response to drugs. In @entity1 @entity3620 , @entity876 is generally not mutated, but may be inactivated by SV40 early region T antigen (SV40 Tag). However, the function of @entity876 has not been investigated in @entity2615 cells. Here, we investigated the function of the cell cycle checkpoints in six @entity1 @entity2615 cell lines (HMCLs) by studying the cell distribution in the different phases of the cell cycle by flow cytometry, and expression of cell cycle proteins, @entity876 , @entity994 ( @entity994 / @entity994 ) and @entity5438 ( @entity13525 ). In addition, we studied @entity876 gene mutations and expression of SV40 Tag. After exposure to gamma-radiation, HMCLs were arrested either in one or both phases of the cell cycle, demonstrating a heterogeneity in cell cycle control. G1 arrest was @entity994 ( @entity994 / @entity994 )- and @entity876 -dependent. Lack of arrest in G1 was not related to @entity876 mutation or binding to SV40 Tag, except in one HMCL presenting a missense mutation at codon 248. These results may help us to understand @entity2615 and develop new treatments.
