Glycogen synthase phosphatase interacts with @entity1457 factor to activate XXXX gene transcription in @entity375 .

@entity60331 @entity9787 @entity1457 @entity70851 @entity5999 @entity48352 @entity12091 @entity563 @entity1889 @entity375
Upon @entity1457 , transcription of many stress-inducible genes is rapidly and dramatically stimulated by @entity1457 factor (HSF). A central region of the @entity375 HSF (designated HSFrr for "repression region") was previously identified and proposed to be involved in repressing the activation domain under non-heat-shock conditions. Here, we used the phage display system to isolate proteins that interact with HSFrr. This should identify factors that modulate HSF activity or directly participate in HSF-mediated transcriptional activation. We constructed a randomly sheared @entity375 genomic library to express @entity375 proteins on the surface of @entity5999 . HSFrr binding phages were selected by cycles of affinity chromatography. DNA sequencing identified an HSFrr-interacting phage that contains the @entity70851 gene. The @entity70851 gene encodes the regulatory subunit for a type 1 @entity563 / @entity1889 phosphoprotein phosphatase, @entity9787 . Both @entity70851 and @entity9787 mutations had little effect on HSF activation of gene transcription of two @entity1457 genes, @entity60331 and @entity48352 . In contrast, @entity1457 induction of @entity12091 gene expression was completely abolished in a @entity9787 mutant and reduced in a @entity70851 mutant. The results demonstrate that the @entity9787 phosphatase and its @entity70851 regulatory subunit play positive roles in HSF activation of @entity12091 transcription.
