Comparative study of four interleukin 17 cytokines of XXXX @entity32453 : Genomic structure, expression pattern, and promoter activity.

@entity72201 @entity462 @entity72200 @entity32453 @entity2348 @entity4515
UNASSIGNED: The interleukin (IL)-17 cytokine family participates in the regulation of many cellular functions. In the present study, we analyzed the genomic structure, expression, and promoter activity of four IL-17 members from the teleost fish @entity32453 ( @entity32453 ), i.e. @entity72200 @entity72201 , CsIL-17F, and IL-17F like (IL-17Fl). We found that @entity72200 , @entity72201 , CsIL-17F, and CsIL-17Fl share 21.2%-28.6% overall sequence identities among themselves and 31.5%-71.2% overall sequence identities with their counterparts in other teleost. All four CsIL-17 members possess an IL-17 domain and four conserved @entity462 residues. Phylogenetic analysis classified the four CsIL-17 members into three clusters. Under normal physiological conditions, the four CsIL-17 expressed in multiple tissues, especially non-immune tissues. @entity2348 upregulated the expression of all four CsIL-17, while viral infection upregulated the expression of @entity72201 and CsIL-17Fl but downregulated the expression of @entity72200 and CsIL-17F. The 1.2  kb 5'-flanking regions of the four CsIL-17 exhibited apparent promoter activity and contain a number of putative transcription factor-binding sites. Furthermore, the promoter activities of @entity72200 , @entity72201 , and CsIL-17F, but not CsIL-17Fl, were modulated to significant extents by lipopolysaccharide, PolyI:C, and @entity4515 . This study provides the first evidence that in teleost, different IL-17 members differ in expression pattern and promoter activity.
