A clinically relevant model of perinatal global XXXX @entity75 in @entity35 .

@entity1 @entity1249 @entity63 @entity168 @entity73 @entity75 @entity10371 @entity519 @entity1763 @entity35 @entity4124 @entity528
We have designed a clinically relevant model of perinatal @entity1763 providing intrapartum @entity519 in @entity35 . On gestation day 22 @entity168 @entity35 were anesthetized and the uterine horns were exteriorized and placed in a water bath at 37  C for up to 20min. After this, pups were delivered from the uterus and manually stimulated to initiate breathing in an incubator at 37  C for 1 h in air. Brains were harvested and stained with @entity10371 , @entity4124 , and TUNEL to detect morphological and apoptotic changes on postnatal days (PND) 1, 3, and 7. Separate cohorts were maintained until PND 50 and tested for learning and memory using Morris water maze (WM). Survival rate was decreased with longer @entity519 time, and 100% mortality was noted when @entity519 time was beyond 18min. Apoptosis was increased with the duration of @entity519 with @entity528 and cell shrinkage in the @entity1249 of hippocampus. The time taken for the juveniles to locate the hidden platform during WM was increased in animals subjected to @entity519 . These data demonstrate that perinatal @entity63 leads to @entity528 in the hippocampus and long-lasting @entity73 . This model mimics @entity519 @entity63 @entity75 in @entity1 and may be appropriate for investigating therapeutic interventions.
