Genes expressed in coronary thrombi are associated with ischemic time in XXXX with @entity583 .

@entity1 @entity32 @entity2289 @entity101 @entity6 @entity1476 @entity310 @entity687 @entity2842 @entity2843 @entity8729 @entity8923 @entity164 @entity373 @entity583 @entity3479 @entity40590 @entity2290
INTRODUCTION: Reports on the content of aspirated coronary thrombi have until now mainly focused on cellular components. We investigated the genetic expression of selected mediators and proteases actively involved in the pathophysiological process of @entity583 in aspirated coronary thrombi. MATERIALS AND METHODS: In this cross-sectional study, RNA from coronary thrombi in 67 subjects with @entity583 was isolated. Gene expression arrays of selected markers were performed by RT-PCR with relative quantification. RESULTS: Twenty of 22 markers were expressed in>50% of the samples. The relative quantification of @entity8729 correlated negatively to total ischemic time (p=0.01), while genes related to fibrinolysis ( @entity3479 , @entity2290 , @entity2289 ), @entity32 ( @entity40590 , @entity373 , @entity2842 , @entity8923 , @entity310 ) and plaque instability ( @entity1476 and @entity2843 ) correlated positively to total ischemic time (all<0.05). Long ischemic time (>4.0hours) associated with a relative reduction in the expression of @entity8729 and a relative increase in the expression of @entity3479 , @entity2290 , @entity2289 , @entity40590 , @entity373 , @entity2842 , @entity8923 , @entity310 , @entity1476 and @entity2843 . The presence of @entity164 2 @entity6 associated with 3.2-fold increased @entity2289 expression (adjusted p=0.033), while the presence of @entity101 associated with about 50% reduction of @entity687 and @entity2843 . Smoking and overweight did not affect any markers. CONCLUSIONS: The gene expression profile from coronary thrombi differed according to ischemic time, shown by reduced content of platelet markers and increased content of fibrinolytic, inflammatory and plaque instability mediators over time. @entity1 with @entity164 2 @entity6 showed increased expression of @entity2289 , indicative of reduced fibrinolysis.
