Can Platelet rich plasma stimulate XXXX ACL growth in culture? A preliminary experience.

@entity1 @entity1423 @entity4404 @entity2667 @entity98 @entity2241
INTRODUCTION: Platelet Rich Plasma (PRP) contains numerous growth factors; @entity1423 ( @entity1423 ) is plasma proteins without platelets, containing growth factors other than platelet derived. We planned to evaluate the effect of both autologous PRP _ @entity1423 on @entity1 ACL cell growth characteristics in culture conditions to see if one was better than the other. METHODS: ACL remnants were collected from eleven @entity1 during ACL reconstruction surgery; @entity1423 and PRP were prepared from blood of these @entity1 . Cells were isolated, identified and cultured and were then divided into six groups. Groups A-D had @entity2241 ( @entity2241 ) added to them along with different concentrations of PRP and @entity1423 . Groups E and F had 5% and 10% PRP respectively but lacked @entity2241 . Cell viability was assayed by @entity98 and @entity4404 assay, and DNA content was evaluated by @entity2667 staining and flow cytometry. RESULTS: analysis of cultured cells showed that addition of PRP (5 or 10%) increased the viability of ACL cells in 4 out of 11 and promoted cell proliferation in 8 of 11 donor samples; 10% PRP was more effective than 5% PRP. However, the difference in effectiveness of 10% PRP was not significantly better than 5% PRP. 5% @entity1423 had no significant effect on cell viability, but it led to an increase in DNA content in 5 of 11. There was no statistically significant effect of either PRP or @entity1423 in preventing cell death (depicted by apoptosis rate). CONCLUSION: PRP may have an enhancing effect on ACL cell viability and promotion of cell proliferation but the ideal concentration of PRP for these positive effects needs to be determined before it could be used in clinical settings for enhancing primary repair of torn ACL. Also larger, more controlled and better studies are needed to confirm its clinical utility.
