Repairing @entity95 radial defects by combining bone XXXX stem cells with bone scaffold material comprising a core-cladding structure.

@entity95 @entity28687 @entity7050 @entity79 @entity2454 @entity236
UNASSIGNED: We prepared a bone scaffold material comprising a @entity7050 / @entity28687 core and a Type I collagen cladding, and recombined it with bone @entity236 stem cells (BMSCs) to evaluate its potential for use in bone tissue engineering by in vivo and in vitro experiments. @entity7050 / @entity28687 without a cladding was used for comparison. The adherence rate of the BMSCs to the scaffold was determined by cell counting. Cell proliferation rate was determined by the @entity2454 method. The osteogenic capability was evaluated by alkaline phosphatase activity. The scaffold materials were recombined with the BMSCs and implanted into a large segmental @entity95 radial defect model to evaluate defect repair. Osteogenesis was assessed in the scaffold materials by histological and double immunofluorescence labeling, etc. The adherence number, proliferation number, and alkaline phosphatase expression of the cells on the bone scaffold material with core-cladding structure were significantly higher than the corresponding values in the @entity7050 / @entity28687 composite scaffold material (P < 0.05). An in vivo test indicated that the bone scaffold material with core-cladding structure completely degraded at the bone defect site and bone formation was completed. The @entity95 large sentimental radial defect was successfully repaired. The degradation and osteogenesis rates matched well. The bone scaffold with core-cladding structure exhibited better osteogenic activity and capacity to repair a @entity79 compared to the @entity7050 / @entity28687 composite scaffold. The bone scaffold with core-cladding structure has excellent physical properties and biocompatibility. It is an ideal scaffold material for bone tissue engineering.
