Factor from @entity35 liver with antiinvasive potential on XXXX ascites @entity157 cells.

@entity5260 @entity5 @entity3 @entity35 @entity157 @entity1068
@entity5260 / @entity1068 extract of normal @entity35 liver, both in vitro and in vivo, inhibited the invasion by a highly invasive subpopulation of @entity35 ascites @entity157 cells, AH 130 (LC-AH cells). The addition of 10-80 micrograms/ml extract inhibited the formation of penetrated colonies of LC-AH cells underneath the cultured mesothelial cell (M-cell) monolayer. The @entity5 cells pretreated with the extract showed the diminished colony formation. Preincubation of the extract with plasma membranes prepared from LC-AH cells abolished the effect of the extract, suggesting a binding of the inhibitory entity [tentatively termed as "invasion-inhibiting factor" (IIF)] to the @entity5 cell surface. The extract did not inhibit the growth of LC-AH cells, but suppressed their directed migration underneath the M-cell monolayer. A concomitant i.p. injection of the extract with LC-AH cells into @entity35 prevented the invasion by @entity5 cells of the peritoneum and formation of @entity5 nodules in the peritoneum and mediastinum, indicating that IIF inhibited the @entity5 cell invasion and @entity3 in vivo, as well. Upon ultrafiltration and gel fractionation, about 60% of IIF activity was recovered in the fraction corresponding to the molecular weight in the range of Mr 3000-4000. This activity was heat-stable at 100 degrees C at neutral pH but labile at acidic pH and was inactivated by the treatment with pronase. The rest of the activity of IIF was found in the fraction of more than Mr 25,000.
