MicroRNAs involved in XXXX stem cells.

@entity108 @entity5956 @entity96 @entity5 @entity73071 @entity107 @entity34323 @entity73072 @entity35 @entity157
BACKGROUND: The existence of @entity5 stem cells in @entity157 ( @entity157 ) has been verified by characterizing side population (SP) cells based on efflux of @entity5956 dye from stem cells. Recent advances in microRNA (miRNA) biology have revealed that miRNAs play an important role in embryonic development and tumorigenesis. However, it is still unclear which miRNAs participate in the @entity96 stem cells (LCSCs) during hepatocarcinogenesis. METHODS: To identify the unique set of miRNAs differentially regulated in LCSCs, we applied SP sorting to primary cultures of F344 @entity35 @entity157 @entity5 cells treated with @entity107 ( @entity108 ) and normal syngenic fetal liver cells, and the stem-like characteristics of SP cells were verified through detecting expression of CD90.1, AFP and CK-7. Global miRNA expression profiles of two groups of SP cells were screened through microarray platform. RESULTS: A total of 68 miRNAs, including @entity73071 , @entity34323 , miR-470*, miR-34c-3p, and let-7i*, were identified as overexpressed in SP of @entity157 cells compared to fetal liver cells. Ten miRNAs were underexpressed, including @entity73072 * and miR-148b*. These miRNAs were validated using stem-loop real-time reverse transcriptase polymerase chain reaction (RT-PCR). CONCLUSIONS: Our results suggest that LCSCs may have a distinct miRNA expression fingerprint during hepatocarcinogenesis. Dissecting these relationships will provide a new understanding of the function of miRNA in the process of neoplastic transformation of LCSCs.
