Primary genetic investigation of a @entity407 model: molecular characteristics and variants of the XXXX gene in Mongolian gerbil.

@entity407 @entity1 @entity1522 @entity70821 @entity1674 @entity70822 @entity12217 @entity70823 @entity12216 @entity1675 @entity35 @entity165
The objective of this work was to establish a novel Mongolian gerbil (Meriones unguiculatus) @entity407 model and to investigate its susceptibility genetic basis. Two rodent (gerbil and @entity35 ) @entity407 models were induced by feeding a high fat/high- @entity165 (HF/HC) diet. There were significant increases of serum total @entity165 , @entity1674 , low-density lipoprotein @entity165 (LDL-C), and high-density lipoprotein @entity165 (HDL-C) in gerbils within a 4-week modeling period. About 10-30% of >8-month-old individuals developed @entity407 spontaneously. The @entity12217 ( @entity12217 ) gene was cloned by merging a sequence of rapid amplification of cDNA ends (RACE) and nested polymerase chain reaction products. The results revealed an open reading frame of 948   bp, encoding a protein of 298 @entity1522 . The gene without a 5'-UTR region in the first intron was highly homologous to @entity1 @entity1675 and @entity35 @entity12216 . The distribution of expression of the @entity12217 gene in liver, brain, heart, lung, kidney, and adrenal gland was detected by an ABC immunohistochemical procedure. Three single nucleotide polymorphisms (SNPs; @entity70821 , @entity70822 , and @entity70823 ) were first found using PCR-single-strand conformation polymorphism (PCR-SSCP) in a closed population containing 444 animals. Correlation analysis confirmed that new SNPs, age, and gender were associated significantly (P < 0.05) with @entity407 .
