Effects of dietary fats and proteins on @entity35 testicular steroidogenic enzymes and serum XXXX levels.

@entity251 @entity4600 @entity27581 @entity4601 @entity1460 @entity35
It is known that certain dietary fats can modulate @entity35 @entity251 metabolism. In the current study we have investigated testicular steroidogenic enzyme activities and serum @entity251 levels in @entity35 fed diets containing either different protein sources (casein, fishmeal, whey) or different lipid sources ( @entity1460 oil, @entity4600 ( @entity4601 ), seal oil, fish oil, lard). The diets examined reflect different marine oils and proteins which are significant components of Northern Canadian diets. Male @entity35 (42-45 days old, 6 per group), were assigned to specific diets for 42 days. On the 43rd day of the study, @entity35 were sacrificed and blood plasma and testes frozen (-80 degrees C) until analysis. Microsomal steroidogenic enzyme activities (3beta-HSD, 17-OHase, C-17,20-lyase, @entity27581 ) were measured radiometrically. There were no differences in enzyme activities between the three dietary protein sources. In contrast, compared with the standard casein diet, all lipid sources caused reductions in C-17,20-lyase activity (>50%); seal oil and fish oil reduced 17-OHase activity (approximately 30%) and @entity1460 oil, @entity4601 fish oil and lard reduced @entity27581 activity (approximately 30%). No effect on 3beta-HSD activity was evident. Serum @entity251 levels were determined using ELISA kits and were not affected by any diet with the exception of the @entity1460 oil diet which was significantly elevated compared with the casein protein diet. Body and testis weights were not affected by diet. In conclusion, these data demonstrate that some dietary lipid sources caused reductions in testicular 17-OHase and C-17,20-lyase activities but not to the extent that serum T levels were affected, while @entity1460 oil caused elevated serum @entity251 in the absence of elevated steroidogenic enzyme activities.
