Effects of XXXX released from @entity7674 on proliferation of vascular endothelial cells in vitro.

@entity1 @entity7674 @entity7978 @entity7
Allogeneic @entity7674 ( @entity7978 ) was prepared by culturing fibroblasts on a two-layered spongy matrix of hyaluronic acid and atelocollagen. @entity7978 can be cryopreserved and transported to other hospitals in a frozen state. The present study was designed to analyze the amounts of @entity7 ( @entity7 ) released from fibroblasts in fresh and cryopreserved @entity7978 and to investigate the effects of this @entity7 on proliferation of vascular endothelial cells in vitro. The culture medium used in preparing @entity7978 (fresh @entity7978 @entity7978 ) was collected and stored at -30 degrees C for the quantitative analysis of @entity7 . After thawing cryopreserved @entity7978 , it was recultured in a culture medium for 1 week. The culture medium used was collected and stored at -30 degrees C for quantitative analysis of @entity7 . The amounts of @entity7 released from the fresh and cryopreserved @entity7978 into the culture medium were about 610 pg/ml and 640 pg/ml, respectively. This finding suggests that the cryopreserved @entity7978 retains its ability to release @entity7 . Immunohistological analysis indicated that some of the @entity7 adhered to the matrix. @entity1 vascular endothelial cells were cultured in medium mixed with the fresh or cryopreserved @entity7978 @entity7978 . Proliferation of vascular endothelial cells was enhanced by increasing the concentration of both @entity7978 culture medium samples. When antihuman @entity7 antibody was added to the culture medium, the proliferative activity of vascular endothelial cells was reduced. These findings confirm that @entity7 released from @entity7978 promotes proliferation of vascular endothelial cells.
