Fast transmission of alterations in plasma @entity2434 / @entity4291 ratio and lyso XXXX levels into changes of red blood cell membrane phospholipid composition after low density lipoprotein apheresis.

@entity1 @entity2434 @entity165 @entity15002 @entity4291
In order to evaluate whether changes in plasma phospholipid composition are rapidly transmitted to the red blood cell membrane (RBCM) under in vivo conditions, the levels of major phospholipids in plasma, low density and high density lipoproteins (LDL and HDL) as well as in RBCM were determined before (pre), directly after (post) and 2 days after (48 h post) LDL apheresis in six @entity1 with severe hypercholesterolaemia. LDL apheresis induced a 30-70% decrease in plasma and LDL @entity165 and total phospholipid levels within 2-3 h. Concomitantly, the percentages of plasma @entity2434 (PC) and the PC/ @entity4291 (SM) ratio were increased compared to initial values. The percentage of plasma lyso PC (LPC) determined before apheresis in the @entity1 was 30% lower with respect to the mean level of LPC in a normolipidaemic control. For LPC of LDL no differences were observed between normolipidaemia and hypercholesterolaemia. LDL apheresis induced a rise by about one third in the percentage of plasma LPC. At 48 h post, plasma @entity15002 levels reapproached pre-apheresis levels, while the percentages of PC and the PC/SM ratio remained elevated. The pattern of changes induced by apheresis in plasma PC, SM and LPC levels was mimicked by changes in RBCM phospholipids. Strong positive relationships were noted for PC, SM and PC/SM as determined at pre, post and 48 h post between plasma and RBCM. In summary, changes in plasma PC, LPC, and PC/SM ratios as induced by LDL apheresis are rapidly transmitted to the RBCM under in vivo conditions, most probably as a result of phospholipid transfer between both compartments.(ABSTRACT TRUNCATED AT 250 WORDS)
