Tumorigenicity of ten karyotypically distinct cell types present in the XXXX @entity320 cell line MeWo-A.

@entity1 @entity7433 @entity19 @entity5 @entity3809 @entity320
The earliest passage of the @entity1 @entity320 cell line, MeWo-A, consists of ten cell types that can be distinguished on the basis of chromosome markers. Two of these cell types have chromosomes with long @entity3809 ( @entity3809 ) containing sequences derived from the short arm of a chromosome #15. In one cell type the @entity3809 is found on a chromosome #15 and in the other it is on a der(15;10)- @entity3809 chromosome. Four other cell types were identifiable by morphologic differences of the short arm of chromosome #13, whereas, the four remaining cell types were identifiable by the presence of prominent satellites on other chromosomes. This study was directed at assessing the relative @entity7433 properties of the different cell types by injecting different numbers of cells intraperitoneally, subcutaneously, or intravenously into Balb/c @entity19 . The @entity5 and nodules that developed in the peritoneal cavity and lungs were explanted into tissue culture. One hundred metaphase chromosome spreads from each established cell line were analyzed cytogenetically to detect changes in proportions of the different cell types. The cell type containing the der(15;10)- @entity3809 chromosome was present in only 20% of the cells injected, but increased in proportion to between 28% and 98% after growth in @entity19 . Although the degree of selection of the der(15;10)- @entity3809 -containing cell type was influenced by the number of cells injected, the consistent selection of these cells strongly suggests that this cell type has a growth advantage. Because the 15- @entity3809 -containing cell type rarely increased in proportion, it is likely that the @entity3809 by itself can not confer the enhanced @entity7433 phenotype but requires the expression of other sequences present on other MeWo chromosomes to provide the selective growth advantage to the cells in which it is found.
