XXXX in dialysis @entity1 : a preliminary study indicating a possible role of apoptosis.

@entity1 @entity2264 @entity2905 @entity598 @entity281 @entity12
@entity2905 is a common finding in dialysis @entity1 . Since @entity281 rate and mortality associated with @entity281 are high in dialysis @entity1 , @entity2905 may be one of the contributing factors. In the present study, we evaluated the mechanism responsible for @entity2905 in these @entity1 . Lymphocytes isolated from dialysis @entity1 showed increased apoptosis (p < 0.001) when compared to lymphocytes isolated from healthy subjects (healthy subjects, 0.5 +/- 0.2% vs. dialysis @entity1 , 8.8 +/- 0.7% apoptotic cells/field). Sera from dialysis @entity1 promoted lymphocyte apoptosis in a time- and dose-dependent manner. These sera also enhanced lymphocyte DNA fragmentation into multiple integers of 180 base pairs in the form of a ladder pattern. Cellulose @entity598 membranes promoted T cell apoptosis when compared to @entity2264 membranes and to control. Cellulose @entity598 dialysis membranes also appear to promote lymphocyte @entity12 expression. Similarly, dialysis sera enhanced T cell Fas as well as @entity12 expression. Neither the cellulose @entity598 nor @entity2264 membranes could induce @entity12 expression on B cells. Similarly, dialysis sera failed to induce @entity12 expression on B cells. On the other hand, anti- @entity12 antibodies attenuated dialysis sera-induced apoptosis in T as well as B cells. Interestingly, dialysis serum showed a 5-fold increase in @entity12 content when compared with control serum. These results suggest that dialysis-associated factors can induce autocrine death in T cells but the help of activated T cells is required to induce death in B cells.
