The effect of recombinant XXXX @entity1841 on a novel @entity2 spine metastasis model in rodents.

@entity1 @entity1841 @entity5 @entity604 @entity2 @entity35 @entity204 @entity420
UNASSIGNED: @entity2 is the second most prevalent @entity5 . Spinal metastases are found in 30-90% of @entity1 with @entity204 attributed to @entity5 . Due to bony destruction caused by metastases, surgical intervention is often required to restore spinal alignment and stability. While some research suggests that @entity1841 may possess tumorigenic effects, other studies show possible inhibition of @entity5 growth. Thirty-six athymic @entity35 underwent intraosseous injection of lung @entity420 cells into the L5 vertebral body. Cells were pre-treated with vehicle control (Group A) or rhBMP-2 (Group B) prior to implantation. At 4 weeks post-implantation, in vivo bioluminescent imaging (BLI) was performed to confirm presence of @entity5 and quantify signal. Plain radiographs and microComputed Tomography (microCT) were employed to establish and quantitate osteolysis. Histological analysis characterized pathologic changes in the vertebral body. At 4 weeks post-implantation, BLI showed focal signal in the L5 vertebral body in 93% of Group A animals and 89% of Group B animals. Average @entity5 burden by BLI radiance was 7.43   *   10(3) p/s/cm(2) /sr (Group A) and 1.11   *   10(4) p/s/cm(2) /sr (Group B). Radiographs and microCT demonstrated osteolysis in 100% of animals showing focal BLI signal. MicroCT demonstrated significant @entity604 in both groups compared to age-matched controls but no difference between study groups. Histological analysis confirmed @entity5 invasion in the L5 vertebral body. These findings provide a reliable in vivo model to study isolated spinal metastases from @entity2 . Statement of Clinical Significance: The data support the notion that exposure to rhBMP-2 does not promote the growth of A549 @entity2 spine lesions. This article is protected by copyright. All rights reserved.
