Surface expression of minichromosome maintenance proteins provides a novel method for detecting XXXX at risk for developing @entity420 in @entity355 .

@entity1 @entity355 @entity9965 @entity420 @entity1141
PURPOSE: The purpose is to determine whether a novel cell cycle marker, @entity9965 ( @entity9965 ), predicted @entity420 ( @entity420 ) risk in @entity355 ( @entity355 ) and whether this could be used in combination with a surface sampling method. EXPERIMENTAL DESIGN: Archival specimens [30 normal squamous esophagus (NE), 20 gastric antrum (GA), 13 duodenum (D2), 62 @entity355 +/- @entity1141 , and 16 ( @entity420 )] were stained for @entity9965 . Sequential biopsies from nine @entity1 who developed @entity420 during surveillance were compared with 18 matched controls who did not progress. Prospective endoscopic cytological brushings (61 NE, 90 @entity355 +/- @entity1141 , and 11 @entity420 ) were scored as @entity9965 positive or negative. RESULTS: @entity9965 was not expressed on the luminal surface of NE, GA, and D2. In @entity355 , the percentage of surface cells expressing @entity9965 correlated highly with the degree of @entity1141 (P < 0.0001). In @entity1 who developed @entity420 , biopsies before @entity1141 had higher @entity9965 expression than the matched control @entity1 (mean, 28.4 and 3.4% positive cells, respectively, P < 0.0001). In the prospective cohort, the histopathological diagnosis of @entity1141 or @entity420 and the @entity9965 -positive brushings were concordant in 91% of the @entity1 (P < 0.005), and the results correlated with the frequency of cases with surface expression of @entity9965 in the retrospective study (P < 0.0001). CONCLUSIONS: Surface expression of @entity9965 can be used to detect @entity1141 and @entity420 , as well as @entity1 with @entity355 at risk for subsequent development of @entity1141 and @entity420 . A brushing technique combined with @entity9965 staining has the potential to be exploited in surveillance and screening protocols.
