@entity71623 , a Honey Medicinal Mushroom, Armillaria mellea (Higher Basidiomycetes) Component, Inhibits Differentiation and Activation of XXXX Macrophages.

@entity1 @entity5750 @entity5 @entity26 @entity3460 @entity4071 @entity71623 @entity375 @entity71624 @entity4210
@entity71623 (AM) is an @entity71624 compound isolated from honey medicinal mushroom, Armillaria mellea, which has anti- @entity5 potential. This study was designed to examine the effects of AM on differentiation and activation macrophages, the major ontogeny of innate immunity. Macrophages were derived from @entity5750 + monocytes which were sorted from @entity1 peripheral blood mononuclear cells. Cell viability was assessed by @entity3460 exclusion test. Cells were stained with Liu's dye for observation of morphology. Expression of surface antigens was examined by flow cytometric analysis. Phagocytosis and generation of reactive @entity26 species (ROS), as functional assays, were evaluated by counting engulfed @entity375 and DCFH-DA reaction. The viability of macrophages was not significantly reduced by AM. AM at nontoxic concentrations markedly increased cytoplasmic vacuoles. The expression of surface @entity5750 , @entity4071 , @entity4210 , and HLA-DR was suppressed. The phagocytosis function, but not ROS production, of macrophages was inhibited by AM. @entity71623 could inhibit the differentiation and activation of @entity1 macrophages. It may have potential to be developed as a biological response modifier for inflammatory diseases.
