Transplacental effects of @entity7043 on @entity730 and ossification of long bones in XXXX .

@entity19 @entity1184 @entity7043 @entity692 @entity730
The transplacental effects of @entity7043 on the skeleton of neonatal @entity19 has been investigated. Pregnant @entity19 were injected intramuscularly with 0.75 mg @entity7043 daily on days 11-19 of gestation. The @entity19 were allowed to deliver and pups were weighed on days 1, 3, 5, 10, 15, 20 and 30 and compared to controls. Light microscopy of non-decalcified longitudinal sections of bones revealed mineral precipitates throughout the zone of @entity1184 cartilage most prominent on days 1, 3 and 5 and gradually disappearing on days 10-20. Transmission electron microscopy revealed intracellular @entity730 of maturing and @entity1184 chondrocytes in addition to abnormal @entity730 of the interstitial substance. @entity692 crystals were not necessarily associated with matrix vesicles. Scanning electron microscopy of long bones revealed a wide zone of calcified intercartilagenous matrix representing the calcified proliferating and @entity1184 zones. In this area, the calcospherites varied in size (0.5-1.4 mu in diameter) and were found in both longitudinal and transverse septa. No significant differences in the distribution and shape of cartilage matrix vesicles between experimental and control animals was observed. However, the fact that mineral crystals in experimental animals was not necessarily associated with matrix vesicles points to the possibility that this "pathological @entity730 " may utilize a different mechanism than the normal process through matrix vesicles.
