Gene expression profile in thyroid of XXXX overexpressing the @entity859 receptor 2a.

@entity268 @entity712 @entity859 @entity10246 @entity19 @entity5 @entity1378 @entity5651 @entity7424
Mutations of the TSH receptor leading to constitutive activation of the @entity268 cascade are responsible for the development of hot nodules, if arising in a somatic cell, and nonautoimmune @entity1378 , when occurring in a germinal cell. An animal model of constitutive activation of the thyroid @entity268 cascade has been obtained by generating @entity19 expressing the @entity859 receptor (Tg-A2aR) under the control of the thyroglobulin promoter. These @entity19 develop huge @entity5651 and die prematurely due to @entity1378 induced @entity712 . To identify new genes involved in the tumorigenic pathway of the thyroid, we designed a protocol using microarray technology to study the differential expression, between normal and transgenic thyroid, of +/-13,000 genes. A total of 360 genes or expressed sequence tags showed a strong modulation with background corrected values of fluorescence superior to 2-fold change. The modulated genes were classified according to their proposed gene ontology functions. Approximately half of them were up-regulated. The function of the majority of these genes in thyroid physiology is still to be determined. Some of them, like @entity7424 or @entity10246 or 5, may play an important role in the development of thyroid nodules through paracrine mechanisms. This study demonstrates the feasibility of sequentially following the cascade of events leading to the formation of @entity5 such as hot thyroid nodule or hyperfunctional @entity5651 .
