Specific IgG and immune complex responses to parthenogenetic females and eggs of nematode Strongyloides venezuelensis for the diagnosis of immunosuppression in infected XXXX .

@entity35 @entity281 @entity13739 @entity24202
UNASSIGNED: In the present study, antigens from parthenogenetic females and eggs of Strongyloides venezuelensis, or anti-parthenogenetic-female and anti-egg antigens were used to detect specific IgG and immune complex responses, respectively. Serum samples from experimentally infected immunocompetent and immunosuppressed @entity35 were analysed on days 5, 8, 13 and 21 post- @entity281 (dpi). An enzyme-linked immunosorbent assay (ELISA) was performed using alkaline parasite extract for specific IgG detection, and anti-parthenogenetic-female or anti-egg antigens for immune complex detection. The data were analysed using analysis of variance (ANOVA), followed by a Bonferroni test. When parthenogenetic female or egg extracts were used as antigens, specific IgGs were not detected in either immunocompetent or immunosuppressed rats. When anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used, immune complexes were detected for the duration of the @entity281 in immunosuppressed animals and were only detected between 5 and 13 dpi in immunocompetent animals. The duration of @entity281 was not significantly different between the immunocompetent and immunosuppressed groups when anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used. Parthenogenetic female extracts yielded significant differences between antibody and immune complex responses in immunocompetent @entity35 from 5 to 13 dpi, but only on day 5 dpi in immunosuppressed @entity35 . Exposure to @entity24202 egg extract yielded significant differences in both antibody and immune complex detection between immunocompetent and immunosuppressed @entity35 for the duration of the @entity281 . In conclusion, ELISA using alternative antigens may be a successful strategy for identifying immune complexes in serum samples and diagnosing active @entity13739 , particularly under conditions of immunosuppression.
