Mycophenolate mofetil increases adhesion capacity of XXXX cells in vitro.

@entity2940 @entity5 @entity2098 @entity618 @entity3 @entity521 @entity4725
BACKGROUND: The immunosuppressive drug @entity618 ( @entity2098 ) reduces expression of the @entity4725 and thereby prevents attachment of alloactivated leukocytes to donor endothelium. The authors speculated that @entity2098 might further diminish receptors of the immunoglobulin superfamily which, however, act as homophilic binding elements. Because decrease of homophilic adhesion receptors correlates with @entity5 dissemination and @entity3 , @entity2098 could trigger development or recurrence of @entity5 . METHODS: The authors analyzed the influence of @entity2098 on homotypic adhesion receptors and its consequence for @entity5 cell attachment to an endothelial cell monolayer. @entity521 ( @entity521 ) cells, which self-aggregate by means of the homophilic-binding element @entity2940 ( @entity2940 ), were used. Effects of @entity2098 on the 140- and 180-kDa @entity2940 isoforms were investigated quantitatively by flow cytometry, Western blot, and reverse-transcriptase (RT) polymerase chain reaction (PCR). The relevance of @entity2940 for @entity5 cell binding was proven by treating @entity521 with @entity2940 antisense oligonucleotides. RESULTS: @entity2098 profoundly increased the number of adherent @entity521 cells, with a maximum effect at 0.1 microM, compared with controls. Decrease of @entity2940 on the cell surface was detected by flow cytometry. Western blot and RT-PCR demonstrated reduced protein and RNA levels of the 140- and 180-kDa isoforms. Treatment of @entity521 cells with @entity2940 antisense oligonucleotides showed that reduced @entity2940 expression leads to enhanced @entity5 cell adhesion. CONCLUSIONS: @entity2098 decreases @entity2940 receptors, which is associated with enhanced @entity5 cell invasiveness. The authors conclude that an @entity2098 -based immunosuppressive regimen might increase the risk of @entity5 @entity3 if this process is predominantly conveyed by means of homophilic adhesion proteins.
