Insulin Signaling in XXXX -induced @entity1370 : Sensitization during Recovery and Potentiation by Lipid Emulsion.

@entity1 @entity137 @entity10175 @entity1920 @entity584 @entity2306 @entity3446 @entity1341 @entity1370 @entity2916 @entity10412 @entity35 @entity33725 @entity6625 @entity9459 @entity413
BACKGROUND: The impact of local anesthetics on the regulation of @entity413 homeostasis by protein kinase B ( @entity584 ) and @entity1920 -activated protein kinase ( @entity33725 ) is unclear but important because of the implications for both @entity137 and its reversal by IV lipid emulsion (ILE). METHODS: @entity35 received 10 mg/kg @entity1341 over 20 s followed by nothing or 10 ml/kg ILE (or ILE without @entity1341 ). At key time points, heart and kidney were excised. Glycogen content and phosphorylation levels of @entity584 , p70 s6 kinase, s6, insulin receptor substrate-1, @entity9459 , @entity33725 , @entity6625 carboxylase, and @entity2916 2 were quantified. Three animals received Wortmannin to irreversibly inhibit @entity10412 ( @entity10412 ) signaling. Isolated heart studies were conducted with @entity1341 and @entity2306 -a reversible @entity10412 inhibitor. RESULTS: @entity1341 @entity1370 rapidly dephosphorylated @entity584 at S473 to 63    5% of baseline and phosphorylated @entity33725 to 151    19%. @entity33725 activation inhibited targets downstream of @entity1 target of @entity3446 complex 1 via @entity2916 2. Feedback dephosphorylation of @entity10175 to 31    8% of baseline sensitized @entity584 signaling in hearts resulting in hyperphosphorylation of @entity584 at @entity9459 to 390    64% and 293    50% of baseline, respectively. Glycogen accumulated to 142    7% of baseline. Irreversible inhibition of @entity10412 upstream of @entity584 exacerbated @entity1341 @entity1370 , whereas pretreating with a reversible inhibitor delayed the onset of @entity137 . ILE rapidly phosphorylated @entity584 at S473 and T308 to 150    23% and 167    10% of baseline, respectively, but did not interfere with @entity33725 or targets of @entity1 target of @entity3446 complex 1. CONCLUSION: @entity413 handling by @entity584 and @entity33725 is integral to recovery from @entity1341 @entity1370 and modulation of these pathways by ILE contributes to lipid resuscitation.
