Fusion of XXXX stem cells and mesenchymal stem cells contributes to @entity11 .

@entity8236 @entity5 @entity19 @entity10062 @entity31973 @entity11 @entity11432 @entity3804
UNASSIGNED: The ability of @entity5 cells to autonomously generate @entity5 vessels has received considerable attention in recent years. However, the degree of autonomy is relative. Meanwhile, the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on @entity5 neovascularization has not been fully elucidated. The present study aimed to illuminate whether cell fusion between @entity11 stem cells and BMSC is involved in @entity11 . BMSCs were isolated from transgenic @entity19 , of which all nucleated cells express green fluorescent protein (GFP). The immunophenotype and multilineage differentiation potential of BMSC were confirmed. SU3 @entity11 stem/progenitor cells were transfected with red fluorescent protein (SU3- @entity11432 cells). In a co-culture system of BMSC-GFP and SU3- @entity11432 , @entity11432 +/GFP+ cells were detected and isolated by dual colors using @entity10062 . The angiogenic effect of @entity11432 +/GFP+ cells was determined in vivo and in vitro. Flow cytometry analysis showed that BMSC expressed high levels of @entity31973 , C44, and very low levels of @entity8236 and @entity3804 . When co-cultured with SU3- @entity11432 , 73.8% of cells co-expressing @entity11432 and GFP were identified as fused cells in the 5th generation. The fused cells exhibited tube formation ability in vitro and could give rise to a solid @entity5 and form @entity5 blood vessels in vivo. In the dual-color orthotopic model of transplantable xenograft @entity11 that expressed @entity31973 , @entity11432 and GFP were identified as de novo-formed vessels derived from the fused cells. The yellow vessels observed in the @entity5 -bearing @entity19 directly arose from the fusion of BMSCs and SU3- @entity11432 cells. Thus, cell fusion is one of the driving factors for @entity5 neovascularization.
