Molecular cloning, expression of a galectin gene in XXXX @entity11320 and the antibacterial activity of its recombinant protein.

@entity10056 @entity12671 @entity375 @entity11320
UNASSIGNED: Galectins play crucial roles in innate immune responses in invertebrate by recognizing and eliminating microinvaders. In this study, a cDNA encoding a galectin in the @entity11320 ( @entity11320 ) was identified and characterized. A recombinant variant of this lectin, rLvgalectin, was expressed in the model organism @entity10056 and its expression was confirmed by Western blot. Biochemical assays indicated that the recombinant protein antibacterial rLvgalectin activity and was expressed in all of the organism's tested tissues Injection of the bacterium @entity12671 into @entity11320 induced hemocytes upregulation of Lvgalectin. The recombinant Lvgalectin protein (rLvgalectin) could bind various microorganism including Gram-positive bacteria, Gram-negative bacteria and @entity375 . And it revealed antimicrobial activity against the test Gram-positive bacteria, Gram-negative bacteria, but did not inhibit the growth of fungus @entity10056 . Moreover, rLvgalectin could significantly enhance the clearance activity of @entity12671 in vivo. In vivo challenge experiments showed that the recombinant rLvgalectin protein can significantly reduce the mortalities of @entity12671 injection. Furthermore, Compared to their wild-type counterparts, Lvgalectin-silenced shrimp exhibited increased mortality and hemocyte apoptosis, decreased bacterial clearance ability and total hemocyte counts, and stronger expression of Lvp53, LvproPO, LvPEN3, and LvCrustin following @entity12671 challenge. Taken together, these results suggest that galectin is important in the innate immune response of shrimp to pathogens infection.
