Nasal messenger RNA expression of interleukins 2, 4, and 5 in XXXX with @entity2023 .

@entity1 @entity225 @entity2023 @entity1759 @entity73143 @entity56 @entity1199 @entity3646 @entity288
In nasal biopsies from 17 adult @entity1 with @entity2023 and from 10 healthy controls, cytokines were analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR). The time-course study during winter included repeated local allergen provocation with subsequent nasal biopsies as well as biopsies taken during pollen season. The RT-PCR for @entity1759 yielded positive bands in 65 of 71 cases, in which cases mRNA for @entity73143 ( @entity1199 , @entity3646 , and @entity225 ) were thus investigated by means of seminested PCR. @entity3646 mRNA was found almost exclusively in the @entity56 @entity1 . During provocation a significant increase in @entity3646 was noticed compared with controls (p = 0.043). Equally, during the natural pollen season, @entity3646 mRNA expression was significantly higher in @entity1 not using nasal corticosteroids compared with those who did (p = 0.011). No differences in @entity1199 or @entity225 were observed between the groups. These findings also indicate, together with earlier observations of T-cell activation, a phenotype switch toward T-helper 2 (Th2) cells, and the accumulation (homing) of these T cells in the nasal mucosa, that T cells constitute the main source for @entity3646 in the nasal mucosa. Therefore, @entity56 @entity1 have an increased synthesis of @entity3646 when provoked with the allergen, and during natural pollen season this synthesis can be downregulated by @entity288 . Furthermore, this study exemplifies the versatility of molecular biology in surgical pathology and that even low-copy-number cytokine mRNA can be examined in routinely snap-frozen surgical specimens.
