The effects of a new therapeutic XXXX /copolymer/ @entity3163 dentifrice on oral bacteria, including odorigenic species.

@entity1 @entity3163 @entity3884 @entity14789 @entity692 @entity3287 @entity3957 @entity1388
This investigation examined the in vitro and ex vivo antimicrobial effects of a new dentifrice, Colgate Total Advanced Fresh, formulated with @entity14789 /copolymer/ @entity3163 , on oral bacteria, including those odorigenic bacteria implicated in bad breath. The effects of Colgate Total Advanced Fresh were compared to commercially available @entity1388 dentifrices that served as controls. Three experimental approaches were undertaken for these studies. In the first approach, the dentifrice formulations were tested in vitro against 13 species of oral bacteria implicated in bad breath. The second approach examined the antimicrobial activity derived from dentifrice that was adsorbed to and released from @entity692 disks. In this approach, dentifrice-treated @entity692 disks were immersed in a suspension of bacteria, and reduction in bacterial viability from the release of bioactive agents from @entity692 was determined. The third approach examined the effect of treating bacteria immediately after their removal from the oral cavity of 11 adult @entity1 volunteers. This ex vivo study examined the viability of cultivable oral bacteria after dentifrice treatment for 2 minutes. Antimicrobial effects were determined by plating Colgate Total Advanced Fresh and control-dentifrice-treated samples on enriched media (for all cultivable oral bacteria) and indicator media (for @entity3884 - @entity3287 -producing organisms), respectively. Results indicated that the antimicrobial effects of Colgate Total Advanced Fresh were significantly greater than either of the other dentifrices for all 13 oral odorigenic bacterial strains tested in vitro (P < or = 0.05). In the second approach, Colgate Total Advanced Fresh-treated @entity692 disks were significantly more active in reducing bacterial growth than the other dentifrices tested (P < or = 0.05). Finally, ex vivo treatment of oral bacteria with Colgate Total Advanced Fresh demonstrated a 90.9% reduction of all oral cultivable bacteria and a 91.5% reduction of oral bacteria producing @entity3957 compared with the control dentifrice. In conclusion, these results, taken together with the significant reductions in clinical malodor scores by Colgate Total Advanced Fresh demonstrated in organoleptic studies, strongly suggest that this dentifrice kills the bacteria that are implicated in the cause of bad breath.
