Intravenous transfusion of endothelial progenitor cells reduces XXXX formation after @entity1317 .

@entity455 @entity19 @entity256 @entity1317 @entity731 @entity8233
Endothelial cell damage is one important pathophysiological step of @entity731 and @entity256 after angioplasty. Accelerated reendothelialization impairs @entity8233 formation. We evaluated the role of intravenously transfused endothelial progenitor cells (EPCs) on reendothelialization and @entity8233 formation in a @entity19 model of @entity1317 . Spleen-derived @entity19 mononuclear cells (MNCs) were cultured in endothelial basal medium. A total of 91.8+/-3.2% of adherent cells showed uptake of acetylated low-density lipoprotein (Dil-Ac-LDL) and lectin binding after 4 days. Immunostaining and long-term cultures confirmed the endothelial progenitor phenotype. To determine the effect of stem cell transfusion on reendothelialization, @entity19 received either fluorescent-labeled spleen-derived MNCs or in vitro differentiated EPCs intravenously after endothelial injury of the carotid artery. Transfused cells were strictly restricted to the injury site, and lectin binding confirmed the endothelial phenotype. Homing of transfused cells to the site of injury was only detectable in splenectomized @entity19 . Cell transfusion caused enhanced reendothelialization associated with a reduction of @entity8233 formation. Systemically applied spleen-derived MNCs and EPCs home to the site of @entity1317 , resulting in an enhanced reendothelialization associated with decreased @entity8233 formation. These results allow novel insights in stem cell biology and provide additional information for the treatment of @entity455 and prevention of @entity256 after angioplasty. The full text of this article is available online at http://www.circresaha.org.
