Loss of heterozygosity in primary XXXX using laser capture microdissection and WAVE DNA fragment analysis techniques.

@entity1 @entity5 @entity2 @entity420 @entity982 @entity2220
BACKGROUND: A number of molecular changes observed by varied conventional methods, including loss of heterozygosity (LOH) on chromosome 3, have been associated with primary @entity2 . To further define the locus of chromosome 3p allele loss in @entity2 , we performed LOH study by using innovative laser capture microdissection and WAVE DNA Fragment Analysis. MATERIAL/METHODS: Thirty-eight paired specimens from @entity1 with @entity2220 were used for this study. Formalin-fixed, @entity982 -embedded tissue from normal stromal cells or lymphocytes and @entity420 were collected using laser capture microdissection. DNA was extracted and amplified by PCR using six polymorphic DNA markers for chromosome 3. PCR products were analyzed by both gel electrophoresis and WAVE DNA Fragment Analysis. RESULTS: LOH at 3p22-24 was found in @entity5 cells from twelve out of thirty-eight @entity1 (32%) when analyzed by WAVE DNA Fragment Analysis and LOH was found in @entity5 cells from nine out of thirty-eight @entity1 (23%) when analyzed by gel electrophoresis. LOH was found in normal control from one out of thirty-eight @entity1 . CONCLUSIONS: 1. Our results suggest putative @entity5 suppressor gene(s) is present in a region at 3p22-24, which may play a role in @entity2 . 2. Laser capture microdissection is essential tool for defined LOH studies. 3. WAVE DNA Fragment Analysis is an accurate, sensitive and automated tool for analysis of DNA fragments.
