Establishment of C6 @entity13 models through stereotactic technique for XXXX research.

@entity1 @entity13 @entity5 @entity1711 @entity11 @entity1710 @entity1986 @entity35
OBJECTIVE: To establish C6 @entity13 models using stereotactic technique, and to study effects of @entity1986 ( @entity1986 ) in @entity35 models of @entity11 . METHODS: C6 @entity11 cells were cultured in dulbecco's minimum essential medium (DMEM) cell culture medium. The in vitro C6 cell cultures were stereotaxically implanted into the right caudate nucleus of @entity35 brain. Presence of @entity5 was confirmed with Factor VIII R, @entity1710 - @entity1711 stain, staining of glial fibrillary acid protein, and S-100 immunohistochemistry. After magnetic resonance (MR) scanning and correction of @entity5 location, the models were divided into groups according to the treating time and laser power (2-10 W). Semiconductor laser optical fibers were inserted in @entity5 for @entity1986 . Cortex's temperature conducted from the center target was measured using infrared thermograph, and deep-tissue temperature around the target was measured using a thermocouple. RESULTS: @entity35 C6 @entity11 were inoculated with optimized stereotactic technique. These @entity11 resembled @entity1 @entity11 in terms of histopathological features. Such models are more reliable and reproducible, with 100% yield of @entity5 and no extracranial growth extension. The difference between cortex temperature conducted from center target and deep-tissue temperature around target was not statistically significant. CONCLUSION: The @entity35 C6 @entity13 model established in the study was a perfect model to study @entity1986 of @entity11 . Infrared thermograph technique measured temperature conveniently and effectively. The technique is noninvasive, and the obtained data could be further processed using software used in @entity1986 research. To measure deep-tissue temperature, combining thermocouple with infrared thermograph technique would present better results.
