Experimental immunogenic XXXX of the orbit: transfer of @entity56 with a subset of T lymphocytes.

@entity738 @entity11264 @entity72374 @entity2694 @entity56 @entity35
An experimental model to investigate @entity2694 formation in inbred @entity35 was established. Animals sensitized to @entity72374 (TNP-OA) and challenged retro-orbitally with TNP-OA covalently linked o Sepharose 4B beads specifically developed a granulomatous response. This granulomatous reactivity was passively transferred into normal animals by lymph node cells, but not by serum antibody from sensitized donors. Lymphocytes which transfer @entity738 formation in normal recipients were characterized by cell fractionation and membrane marker analysis. These experiments show that the effector cells capable of transferring @entity56 are enriched in the lower density fractions on discontinuous Percoll gradients. These cells are lymphoblasts and express the @entity11264 helper T lymphocyte marker. It was also demonstrated that lymphoid cells from sensitized donors in the higher density Percoll fraction appear to be incapable of adoptively transferring granulomatous responsiveness directly to normal recipients. However, incubation of these high density lymphocytes with specific antigen resulted in marked enhancement of their ability to transfer the disease. Antigen-induced activation also resulted in an increase in both lymphoblasts and the @entity11264 marker. The authors conclude, therefore, that a subset of T cells which are lymphoblasts and express the helper-cell marker is responsible for @entity738 formation in sensitized animals and is capable of transferring @entity2694 formation to non-sensitized normal recipients.
