Local adenoviral-mediated inducible XXXX synthase gene transfer inhibits neointimal formation in the porcine coronary stented model.

@entity1 @entity1373 @entity842 @entity3623
In this study the effect of local adenoviral-mediated delivery of inducible @entity842 synthase on restenosis was evaluated in a porcine coronary stented model. Local gene transfer of recombinant adenoviral vectors that encode @entity1 inducible @entity842 synthase (AdiNOS) was tested. Control vector (AdNull) lacked a recombinant transgene. Endoluminal delivery of 1.0 x 10(11) adenoviral particles was accomplished in 45 s using the Infiltrator catheter (Interventional Technologies, San Diego, CA). Coronary stents were deployed, oversized by a ratio of 1.2:1, in the treated segments immediately after gene transfer. Fourteen animals were sacrificed at day 28 to evaluate the effects of @entity3623 gene transfer on morphometric indices, and 4 animals were sacrificed at day 4 for detection of @entity1 @entity3623 expression by RT-PCR. @entity3623 mRNA was detected in six of eight @entity3623 -transferred arteries, whereas no expression of @entity1 @entity3623 was detected in the nontarget arteries. Morphometric analysis showed that @entity3623 transfer significantly reduced neointimal formation (3.41 +/- 1.12 mm(2) vs 2.14 +/- 0.68 mm(2), P < 0.05). We concluded that efficient intramural adenovirus-mediated @entity3623 transfer can be achieved by using Infiltrator catheters. @entity3623 gene transfer significantly reduces neointimal @entity1373 following stent injury.
