The XXXX symporter is unlikely to be a thyroid/breast shared antigen.

@entity1 @entity22598 @entity5787 @entity49507 @entity0 @entity1669 @entity21112 @entity38415 @entity435 @entity1374
PURPOSE: @entity5787 ( @entity5787 ) autoantibodies (TPOAb) seem to be protective for @entity1 with @entity0 ( @entity0 ). Thyroid and breast tissues both express the @entity22598 symporter ( @entity49507 ), similarly both have a peroxidase activity, @entity5787 and lactoperoxidase ( @entity38415 ) respectively. We hypothesize a common immune response to a thyroid/breast shared antigen suggesting three putative mechanisms: (1) TPOAb react to both @entity5787 and @entity38415 , (2) @entity5787 could be expressed in @entity0 and (3) @entity1 with TPOAb could have autoantibodies to @entity49507 (NISAb). Previous studies excluded NISAb that block @entity49507 activity in sera of @entity1 with @entity1669 ( @entity1669 ) and/or @entity0 . This study investigates neutral NISAb (binding without affecting function). METHODS: Clones of @entity435 cells stably expressing @entity1 @entity49507 ( @entity49507 ; @entity435 - @entity49507 ) were isolated following transfection of @entity49507 in pcDNA3 vector. Expression of @entity49507 mRNA and surface protein was confirmed by PCR and flow cytometry respectively using a @entity49507 -mouse-monoclonal-antibody. @entity435 - @entity49507 and controls transfected with the empty pcDNA3 vector ( @entity435 -Empty) were incubated with 42 heat-inactivated @entity1 sera followed by an anti-human-IgG- @entity21112 -conjugate: 12 with @entity0 , 11 with @entity1669 , 10 with both @entity0 and @entity1669 and 9 with non-autoimmune @entity1374 . The Kolmogorov-Smirnov Test was used to compare the fluorescence intensity obtained with @entity435 - @entity49507 and @entity435 -Empty, using sera from six young males as a negative control population. RESULTS: None of the 42 sera were positive for NISAb. CONCLUSIONS: NISAb are rare and @entity49507 is unlikely to be a common thyroid/ @entity0 shared antigen. We have recently demonstrated @entity5787 expression in @entity0 tissue and are currently investigating TPOAb cross-reactivity with @entity5787 / @entity38415 .
