A Novel XXXX Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress.

@entity2272 @entity2271 @entity11896 @entity1460 @entity375 @entity968
Water is essential for plant growth and development. @entity11896 leads to loss of yield and decreased crop quality. To understand water transport mechanisms in plants, we cloned and characterized a novel tonoplast intrinsic protein (TIP) gene from @entity1460 with the highest similarity to TIP2-type from other plants, and thus designated GmTIP2;3. The protein sequence contains two conserved NPA motifs and six transmembrane domains. The expression analysis indicated that this gene was constitutively expressed in all detected tissues, with higher levels in the root, stem and pod, and the accumulation of GmTIP2;3 transcript showed a significant response to osmotic stresses, including 20% @entity968 ( @entity968 ) and 100  M @entity2272 ( @entity2271 ) treatments. The promoter-GUS (glucuronidase) activity analysis suggested that GmTIP2;3 was also expressed in the root, stem, and leaf, and preferentially expressed in the stele of root and stem, and the core promoter region was 1000 bp in length, located upstream of the ATG start codon. The GUS tissue and induced expression observations were consistent with the findings in @entity1460 . In addition, subcellular localization showed that GmTIP2;3 was a plasma membrane-localized protein. @entity375 heterologous expression revealed that GmTIP2;3 could improve tolerance to osmotic stress in @entity375 cells. Integrating these results, GmTIP2;3 might play an important role in response to osmotic stress in plants.
