Detection of XXXX -Coated Gold Nanoparticles in Orthotopic @entity788 using Hyperspectral Imaging.

@entity788 @entity137 @entity5 @entity19 @entity2434 @entity3736 @entity3440 @entity420
UNASSIGNED: Nanoparticle uptake and distribution to solid @entity5 are limited by reticuloendothelial system systemic filtering and transport limitations induced by irregular intra-tumoral vascularization. Although vascular enhanced permeability and retention can aid targeting, high interstitial fluid pressure and dense extracellular matrix may hinder local penetration. Extravascular diffusivity depends upon nanoparticle size, surface modifications, and tissue vascularization. Gold nanoparticles functionalized with biologically-compatible layers may achieve improved uptake and distribution while enabling @entity137 through synergistic combination of chemotherapy and thermal ablation. Evaluation of nanoparticle uptake in vivo remains difficult, as detection methods are limited. We employ hyperspectral imaging of histology sections to analyze uptake and distribution of @entity2434 -coated @entity3736 gold nanoparticles (CGN) and @entity3440 -gold nanoshells (SGN) after tail-vein injection in @entity19 bearing orthotopic pancreatic @entity420 . For CGN, the liver and @entity5 showed 26.5  8.2 and 23.3  4.1 particles/100 m2 within 10 m from the nearest source and few nanoparticles beyond 50 m, respectively. The spleen had 35.5  9.3 particles/100 m2 within 10 m with penetration also limited to 50 m. For SGN, the liver showed 31.1  4.1 particles/100 m2 within 10 m of the nearest source with penetration hindered beyond 30 m. The spleen and @entity5 showed uptake of 22.1  6.2 and 15.8  6.1 particles/100 m2 within 10 m, respectively, with penetration similarly hindered. CGH average concentration (nanoparticles/ m2) was 1.09  0.14 in the liver, 0.74  0.12 in the spleen, and 0.43  0.07 in the @entity5 . SGN average concentration (nanoparticles/ m2) was 0.43  0.07 in the liver, 0.30  0.06 in the spleen, and 0.20  0.04 in the @entity5 . Hyperspectral imaging of histology sections enables analysis of @entity2434 -coated gold-based nanoparticles in @entity788 with the goal to improve nanotherapeutic efficacy.
