Flow cytometric measurement of respiratory burst activity and surface expression of neutrophils for septic XXXX prognosis.

@entity1 @entity295 @entity10685 @entity326 @entity1359 @entity21184 @entity2282 @entity281 @entity4071
BACKGROUND: The hyperinflammation that begins with @entity295 is essential for eradicating @entity281 but also causes hypoperfusion and organ failure. To understand the innate immune status of septic @entity1 , the functional and phenotypic changes of neutrophils during @entity295 and their clinical implication were studied. METHODS: Seventy-four @entity1 who were admitted to intensive care unit due to severe @entity295 or @entity326 were enrolled. Surface antigens of neutrophils ( @entity10685 , @entity2282 , and @entity4071 ) were detected by flow cytometry. Respiratory burst activity (RBA) was measured by flow cytometry using @entity21184 and @entity1359 . The parameters were serially examined at Days 1 and 8 in @entity326 @entity1 . RESULTS: High @entity10685 and low @entity2282 and @entity4071 on Day 1 was associated with @entity295 severity (P   =   0.003, 0.017, and 0.007, respectively). On Day 1, RBA and @entity10685 were higher in survivors than in nonsurvivors of @entity326 @entity1 (P   =   0.012 and 0.027, respectively), and on Day 8, @entity2282 and @entity4071 were higher in survivors than in nonsurvivors (P   =   0.019 and 0.036, respectively). High RBA and high @entity10685 on Day 1 showed low 28-day mortality in univariate analysis (P   =   0.018 and 0.034, respectively). In multivariate analysis, RBA maintained statistical significance (P   =   0.042) but @entity10685 revealed only a tendency (P   =   0.064). CONCLUSIONS: Neutrophil surface antigen ( @entity10685 , @entity2282 , and @entity4071 ) could reflect @entity295 severity. High @entity10685 expression and high RBA at early phase of @entity295 might be associated with better prognosis, whereas high expression of @entity2282 and @entity4071 at late phase of @entity295 might be associated with better prognosis.    2015 International Clinical Cytometry Society.
