XXXX modulates sympathetic neurotransmission at the prejunctional level.

@entity1110 @entity842 @entity2224 @entity11571 @entity2922 @entity35
In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced @entity842 (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and neuronal NO synthase (NOS) along the vascular tree of the heart at the light and electron microscopic level using @entity1110 -diaphorase ( @entity1110 -d) staining as a marker for NOS. In addition, the functional effects of exogenous NO on coronary vascular resistance and cardiac adrenergic nerves was studied using the isolated perfused @entity35 heart as a model. The intraaxonal @entity2922 content of adrenergic nerve fibers was visualised and morphometrically assessed by applying @entity11571 -induced histofluorescence. The expression of endothelial NOS in the heart was found to depend on the diameter of the blood vessel. Arteries > 100 microns always showed intense staining, whereas staining in smaller arteries and veins was considerably weaker. Smooth-muscle free vessels were essentially devoid of @entity1110 -d activity. In @entity2224 , neuronal NOS localised in autonomic nerve fibers along the entire vascular tree. Ultrastructurally, @entity1110 -d staining revealed adjacent localisation of NOS-positive and -negative axons, suggesting and interaxonal modulation of adjacent autonomic nerve fibers by NO. In isolated perfused @entity35 hearts, the intracoronary application of 10(-8) M NO produced a marked decrease of coronary perfusion pressure, which was accompanied by a distinct increase in intraaxonal @entity2922 levels of intramural adrenergic nerve fibers.(ABSTRACT TRUNCATED AT 250 WORDS)
