Altered expression profile of @entity224 dismutase isoforms in nasal polyps from nonallergic XXXX .

@entity1 @entity4723 @entity26 @entity39051 @entity842 @entity1412 @entity224 @entity1367
OBJECTIVE/HYPOTHESIS: @entity1412 ( @entity1412 ) is a chronic inflammatory disease of the upper respiratory tract. The pathophysiology is unknown but has been shown to be multifactorial. Free radical-mediated damage has been implicated in the pathogenesis of @entity1412 . @entity224 dismutases (SODs) are the first and the most important line of antioxidant enzyme defense against reactive @entity26 species. Moreover, isozymes of the SOD family are critical for modulating the activity of @entity842 , a gaseous free radical that is believed to play roles in the physiology and pathology of respiratory tracts. However, the expression profile of SOD isoforms in @entity1412 remains unclear. We aimed to investigate the expression profile of the SOD isoforms in nasal polyps from nonallergic @entity1 . STUDY DESIGN: Prospective study. METHODS: Nasal polyp tissues were obtained from eight nonallergic @entity1 who underwent elective polypectomy; mucosal specimens from the middle turbinates were acquired from eight subjects without @entity1412 as control tissues. The expression profile of SOD isoenzymes, @entity1367 , @entity4723 , and @entity39051 , in the nasal tissues was determined by reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and Western blotting (WB). RESULTS: @entity1412 in all eight of the @entity1412 @entity1 manifested as severe or recurrent sinonasal @entity1412 clinically. The expression pattern of SOD isoenzymes evaluated by RT-PCR analysis indicated that the mean levels of @entity1367 mRNA and, to a greater extent, @entity39051 mRNA were higher in polyp tissues than in control tissues. There was no significant difference in the expression levels of @entity4723 mRNA between the two groups. The data from ELISA and WB analysis showed that there were increased expressions of @entity1367 and @entity39051 protein in polyp tissues compared with the control tissues, but there was no difference in the expression of @entity4723 protein between the two groups. The results from RT-PCR, ELISA, and WB were paralleled and revealed that the expressions of @entity1367 and, to a greater extent, @entity39051 were higher in polyp tissues than in the control group. CONCLUSIONS: The expressions of @entity39051 and @entity1367 were higher in polyp tissues. These results are consistent with previously reported data and support the hypothesis that there is increased oxidative stress in @entity1412 . Our data also suggest that the SODs might be important in the pathogenesis of @entity1412 ; however, the roles these SOD isoforms, especially @entity39051 , play in both normal nasal mucosa and @entity1412 require further clarification.
