Metastatic behavior of @entity5 cells isolated from primary and metastatic @entity1 XXXX implanted into different sites in @entity19 .

@entity1 @entity19 @entity5 @entity2 @entity3 @entity14
The purpose of these studies was to examine the growth characteristics and metastatic behavior of freshly isolated @entity1 @entity14 implanted into athymic @entity19 . Four @entity5 lines were derived from primary @entity14 , three lines from hepatic @entity3 , and one line from a @entity3 to a mesenteric lymph node. Subsequent to implantation into the subcutis or the muscularis, @entity5 lines derived from @entity3 grew faster in the @entity19 than did cells isolated from the primary @entity5 . Regardless of the source of the cells, however, little or no visceral organ @entity3 was found. Subsequent to i.v. injection, experimental lung colonies could be produced by some of the cells, but there was no correlation between @entity2 colony formation and the origin of the @entity1 colorectal cells. The intrasplenic injection of @entity14 cells provided a useful procedure to identify @entity1 @entity14 cells with metastatic potential to liver. Extensive @entity5 burdens in the liver were observed as early as 30 days after injection with two of the three liver @entity3 -derived @entity5 lines. No liver @entity3 were found after the intrasplenic injection of cells isolated from the lymph node-derived @entity5 line. Ninety days after the intrasplenic injection of cells from the four primary @entity14 , limited liver @entity3 were observed. We conclude that @entity3 of @entity1 @entity14 can be studied in @entity19 , and its outcome depends upon both the intrinsic metastatic capacity of the @entity1 @entity5 cells and the organ environment of implantation.
