@entity35 brain pericyte cell lines expressing beta2-adrenergic receptor, XXXX receptor type 1A, klotho, and @entity9018 mRNAs despite having endothelial cell markers.

@entity9018 @entity43012 @entity101 @entity26081 @entity25603 @entity1809 @entity1808 @entity12186 @entity35 @entity15
Pericytes are an integral component of blood capillaries, but their involvement in a variety of conditions and diseases, including @entity101 and @entity15 , is poorly understood. In order to analyze the mRNA expression of markers related to @entity101 and @entity15 in @entity35 brain pericytes, we have established brain capillary pericyte cell lines from temperature-sensitive SV40 large T antigen transgenic @entity35 . The newly established clones showed similar biochemical and morphological properties to primary pericytes. The expression of endothelial cell-related markers @entity25603 , @entity12186 , @entity43012 , and @entity26081 was evaluated by RT-PCR analysis. beta2-Adrenergic receptor (beta2-AR), @entity1809 ( @entity1809 ), and klotho were also evaluated as markers related to @entity101 and @entity15 . All of the isolated clones expressed beta2-AR, @entity1809 and klotho genes. They also stably expressed @entity25603 and @entity26081 , while @entity12186 , @entity43012 and @entity9018 were expressed only at low levels in some of the clones. The expressions of @entity1809 in TR-PCT1 were determined by Western blotting. @entity1808 stimulated migration of pericytes. This effect was blocked by an @entity1809 antagonist. The pericyte cell lines established here are pluripotent, and should be useful for analysis of the reactivity and biological roles of pericytes.
