High XXXX dialysis solutions increase synthesis of vascular endothelial growth factors by peritoneal vascular endothelial cells.

@entity2656 @entity1 @entity298 @entity7 @entity880 @entity35 @entity413
OBJECTIVE: Increased peritoneal vasculature has been reported in long-term @entity298 ( @entity298 ), and vascular endothelial growth factors (VEGFs) have been found in dialysate. High concentrations of @entity413 or @entity880 , @entity413 degradation products (GDPs), and low pH of dialysis solutions are all possible factors in increased peritoneal @entity7 synthesis. In this study, we investigated the effects of high @entity413 dialysis solutions on @entity7 synthesis by peritoneal vascular endothelial cells (PVECs). METHODS: The PVECs were isolated from @entity35 omentum and were incubated for 4 hours in three different culture media [M199 media (control), conventional dialysis solutions containing 4.25% @entity413 diluted with an equal volume of M199 media (HGD), and M199 media containing 118 mmol/L @entity2656 as an osmolar control ( @entity2656 )]. Levels of @entity7 protein in the culture supernatant were measured by ELISA, and mRNA expression was determined by Northern blot analysis. Data are presented as percent of control. RESULTS: After incubation for 4 hours, the number of cells did not differ between the 3 groups. Levels of @entity7 in culture supernatant were significantly higher in the HGD group (124% +/- 19%, p = 0.006) as compared with the control and @entity2656 (85% +/- 10%) groups. The mRNA expression of @entity7 appeared to be higher in the HGD group (128% +/- 49%) than in the control and @entity2656 (94% +/- 18%) groups. CONCLUSION: High @entity413 dialysis solutions increased @entity7 synthesis by PVECs. The relationship between @entity7 synthesis by PVECs and neovascularization of the peritoneum observed in long-term @entity298 @entity1 has to be studied further.
