Effect of Small Interference RNA on the XXXX -Sensitive @entity972 Channel in H9c2 Cells.

@entity2643 @entity66969 @entity1617 @entity955 @entity972 @entity35 @entity934
BACKGROUND: The @entity934 -sensitive @entity972 channel (IK( @entity1617 )) expresses only in the atrium, not in the ventricle. The main component of the @entity35 IK( @entity1617 ) channel is the Kir3.4 protein, and its encoding gene is the @entity66969 gene. IK( @entity1617 ) inhibitors provide a promising treatment for @entity955 without side effects on the ventricle. At present, however, there is no specific IK( @entity1617 ) inhibitor. Small interference RNA (siRNA) has the advantages of high specificity and high efficiency. Our aim is to use siRNA to inhibit the IK( @entity1617 ) channel on H9c2 cells derived from @entity35 cardiomyocytes. METHODS: Three pairs of siRNAs targeting the @entity35 IK( @entity1617 ) channel were synthesized. H9c2 cells were divided into six groups: control, negative siRNA control, siRNA sequence 1, siRNA sequence 2, siRNA sequence 3, and @entity2643 . The @entity66969 gene and Kir3.4 protein were measured, and IK( @entity1617 ) currents were recorded by patch-clamp. RESULTS: The negative siRNA control and siRNA sequence 1 had no inhibitory effects on the IK( @entity1617 ) channel. siRNA sequences 2 and 3 could cause a significant decrease in @entity66969 gene expression, Kir3.4 protein, and IK( @entity1617 ) currents (p<0.05). @entity2643 could inhibit the IK( @entity1617 ) currents but had no effect on @entity66969 gene expression and Kir3.4 protein. CONCLUSION: siRNA could effectively inhibit the IK( @entity1617 ) channel and is a potential therapy for @entity955 .
