Primary resistance of XXXX @entity1534 to antimicrobial agents in Polish @entity1 .

@entity1 @entity868 @entity115 @entity4581 @entity26121 @entity1534 @entity803
@entity1534 resistance to antimicrobial agents is an important factor compromising the efficacy of treatment. Therefore the aims of our study were: to determine the prevalence of @entity1534 resistance to @entity4581 , @entity868 , @entity115 and @entity803 in @entity1 prior to eradication therapy, to compare different methods of susceptibility testing and to detect mutations responsible for @entity4581 resistance. During 1996-2000, 259 @entity1534 strains were isolated from antral gastric biopsies. Susceptibility to antimicrobials was determined by the agar dilution method and the Etest. Mutations in the 23S rRNA gene associated with @entity4581 resistance were analysed by PCR-RFLP and direct sequencing. Overall, ninety-six strains (37%) were resistant to @entity868 , 50 strains (19.3%) were resistant to @entity4581 , and 20 strains (7.7%) were simultaneously resistant to both drugs. All cultured isolates were sensitive to @entity115 and only one isolate (0.4%) was resistant to @entity803 . The agar dilution method and the Etest showed a perfect category correlation for @entity4581 and 4% discrepancies for @entity868 . Primary resistance to @entity4581 was mainly associated with an @entity26121 mutation in the 23S rRNA gene of @entity1534 . The study highlights the high prevalence of @entity1534 primary resistance to @entity4581 in Polish @entity1 , which implies a need for pretreatment susceptibility testing.
