A sensitive and efficient method for determination of XXXX and @entity3167 in breast milk and milk-based products by high-performance liquid chromatography via UV detection and mass spectrometry identification.

@entity72584 @entity3167 @entity33944 @entity33149 @entity11749 @entity9928 @entity14111 @entity48777 @entity4826 @entity7787 @entity4825
UNASSIGNED: A sensitive and efficient method of high performance liquid chromatography using @entity72584 ( @entity14111 ) as pre-column derivatization reagent coupled with UV detection (HPLC-UV) and online mass spectrometry identification was established for determination of the most common @entity48777 ( @entity4825 ( @entity4826 ) and @entity11749 (GalNAc)) and @entity3167 ( @entity33944 ). In order to obtain the highest liberation level of the three @entity7787 without destruction of @entity33944 or conversion of @entity4826 /GalNAc to GlcN/ @entity33149 in the hydrolysis procedure, the pivotal parameters affecting the liberation of @entity48777 / @entity33944 from sample were investigated with response surface methodology (RSM). Under the optimized condition, maximum yield was obtained. The effects of key parameters on derivatization, separation and detection were also investigated. At optimized conditions, three @entity7787 were labeled fast and entirely, and all derivatives exhibited a good baseline resolution and high detection sensitivity. The developed method was linear over the calibration range 0.25-12 M, with R(2)>0.9991. The detection limits of the method were between 0.48 and 2.01pmol. Intra- and inter-day precisions for the three @entity7787 ( @entity4826 , GalNAc and @entity33944 ) were found to be in the range of 3.07-4.02% and 3.69-4.67%, respectively. Individual @entity9928 recovery from spiked milk was in the range of 81%-97%. The sensitivity of the method, the facility of the derivatization procedure and the reliability of the hydrolysis conditions suggest the proposed method has a high potential for utilization in routine trace @entity48777 and @entity33944 analysis in biological samples.
