Origin of the Hodgkin/Reed-Sternberg cells in XXXX with "Hodgkin's transformation".

@entity1 @entity706 @entity5 @entity377 @entity1072 @entity6492 @entity978 @entity7513
@entity706 with the characteristic features of @entity978 ( @entity978 ) occasionally develops in @entity1 with @entity1072 ( @entity1072 ), and has been called @entity6492 with @entity978 features. In such cases, large @entity5 cells have the morphological and immunophenotypic features of classical Hodgkin and Reed-Sternberg (H-RS) cells. However, it is not known whether the H-RS cells arise from transformation of the underlying @entity1072 cells or from a different pathological process. We report herein a study of the clonal relationship between the @entity1072 cells and the H-RS cells in three cases of @entity6492 with @entity978 features by using a single cell assay. We isolated single @entity1072 cells and H-RS cells from immunostained tissue sections by micromanipulation. The @entity7513 ( @entity7513 ) complementarity determining region (CDR) III of each cell was amplified by the polymerase chain reaction (PCR). The products were then compared by gel electrophoresis and @entity377 sequencing. The @entity7513 CDRIII sequences from the H-RS cells were identical to those from the @entity1072 cells in two cases. In one case, the clonal relationship between the two types of cells could not be determined because PCR products could not be obtained from any of the H-RS cells. This study shows that the H-RS cells and the @entity1072 cells belong to the same clonal population in some cases of @entity6492 with @entity978 features. Furthermore, our findings indicate that mature B cells can undergo transformation to cells with the features of H-RS cells, in association with a cellular background typical of @entity978 . This study also supports recent findings suggesting that the H-RS cells in classical @entity978 are derived from transformed B cells.
