Effects of exogenous @entity2493 prior to the breeding season on testis weight and epididymal XXXX receptors in rams.

@entity586 @entity17506 @entity2493 @entity342 @entity587
Rams were randomly assigned to an experiment that evaluated effects of treatment (2.5 mg @entity2493 /d for 45 d vs control; @entity17506 vs CON) in mid-May through June on testis weight and concentration of epididymal @entity342 receptor in July or September (Sept). Mean testis weight of @entity17506 and CON rams was not different in July (332 vs 283 g), but in Sept was less (P less than .05) for @entity17506 rams than CON rams (268 vs 382 g). Testis weight of @entity17506 rams was less (P less than .05) in Sept than in July. Caput, corpus and cauda epididymal tissues were used to prepare extracts which were analyzed for concentration of @entity586 ( @entity587 ) receptor using a new standard curve method. A standard extract was characterized using four independent 8-point Scatchard analyses and found to contain 6.0 fmol @entity587 receptor/mg wet tissue (Ka = 3.5 X 10(8).M-1); this extract was used to establish standard curves for assays of unknown samples. Data on concentration of @entity587 receptors measured by Scatchard analysis and the standard curve method were highly correlated (r = 0.99; P less than .01; n = 8). Concentrations of @entity587 receptor were not affected by treatment, month of castration, or their interaction. However, for data pooled across treatment and month, concentration (fmol/mg protein) of @entity587 receptor was greater (P less than .05) in caput or corpus (125 or 122) than in cauda (92) epididymidis. The regional distribution of epididymal @entity587 receptors in this study confirmed our previous findings.(ABSTRACT TRUNCATED AT 250 WORDS)
