Metabolism of XXXX by different types of cells in the @entity1 ovary.

@entity1 @entity3653 @entity251 @entity12325 @entity1420 @entity3014 @entity53 @entity134 @entity106 @entity10978 @entity21920 @entity8665
1. The metabolism of @entity3653 ( @entity106 ) by primary cultures of @entity1 ovarian cells has been studied to identify the cell type(s) responsible for biotransformation of this carcinogen. The rate of @entity106 metabolism was maximal in granulosa cells prestimulated in vivo with antiestrogen, hMG ( @entity1 menopausal gonadotropin) and hCG ( @entity1 chorionic gonadotropin), i.e., treatments required for maximal oocyte maturation and, thus, granulosa cell proliferation. In cells from unstimulated ovaries, the metabolism was maximal in granulosa- @entity8665 cells isolated from corpus luteum. 2. @entity134 ( @entity1420 and @entity53 ) levels were determined in the spent culture media or in media in parallel with @entity106 metabolism to find out whether elevated @entity134 levels in vivo are required for the rapid metabolism of @entity106 . In granulosa cell cultures from stimulated cycles, the concentrations of both @entity1420 and @entity53 were at least 2 or 3 times higher, respectively, than in any of the other cell types tested. In cell cultures derived from unstimulated ovaries, the @entity1420 and @entity53 concentrations were highest in granulosa- @entity8665 cell cultures. 3. Incubations of granulosa cells with @entity106 in the absence or presence of gonadotropins, @entity251 or anti--hCG were performed to investigate possible hormonal requirements for the @entity12325 system(s) which metabolize @entity106 . No change in the rate of metabolism was obtained with follicle stimulating hormone (FSH), luteinizing hormone (LH), hCG or @entity251 . However, anti-hCG increased this activity about 70%, indicating a negative modulatory role of hCG on @entity106 mono-oxygenase activity in @entity1 granulosa cells. 4. @entity106 mono-oxygenase activity in cell cultures was inhibited about 95% by @entity10978 ( @entity21920 ), an inhibitor of certain @entity12325 -catalysed activities. @entity3014 (BP) was metabolized at the same rate as @entity106 in granulosa cell cultures.
