Tumor microenvironment in XXXX : possible therapeutic targets.

@entity1137 @entity0 @entity5 @entity442 @entity2279 @entity28079 @entity2280
UNASSIGNED: Invasive @entity1137 (IDC and ILC) are the two most common histological types of @entity0 , and have been considered to develop from terminal duct lobular unit but their molecular, pathological, and clinical features are markedly different between them. These differences could be due to different mechanisms of carcinogenesis and @entity5 microenvironment, especially @entity5 -associated fibroblasts (CAFs) but little has been explored in this aspect. Therefore, in this study, we evaluated the status of angiogenesis, maturation of intratumoral microvessels, and proliferation of CAFs using immunohistochemistry and PCR array analysis to explore the differences of @entity5 microenvironment between ILC and IDC. We studied grade- and age-matched, luminal-like ILC and IDC. We immunolocalized @entity2280 and aSMA for an evaluation of CAFs and @entity2279 , @entity28079 , a specific marker of proliferative endothelial cells and nestin, a marker of pericytes for studying the status of proliferation and maturation of intratumoral microvessel. We also performed PCR array analysis to evaluate angiogenic factors in @entity5 stromal components. The number of CAFs, microvessel density, and @entity28079 / @entity2279 positive ratio were all significantly higher in ILC than IDC but nestin immunoreactivity in intratumoral microvessel was significantly lower in ILC. These results did indicate that proliferation of CAFs and endothelial cells was more pronounced in ILC than IDC but newly formed microvessels were less mature than those in IDC. PCR array analysis also revealed that @entity442 expression was higher in ILC than IDC. This is the first study to demonstrate the differences of @entity5 microenvironment including CAFs and proliferation and maturation of intratumoral vessels between ILC and IDC.
