Alterations in the expression of selected MHC antigens in XXXX and @entity957 of the uterine cervix.

@entity1 @entity7722 @entity75 @entity5 @entity6349 @entity957 @entity13620 @entity189 @entity1141 @entity29152 @entity3146 @entity4107
BACKGROUND: The objective of the study was to determine whether there is a correlation between expression of selected major histocompatibility complex (MHC) antigens and the presence of @entity189 and @entity75 of the cervix. We also attempted to determine whether there is a correlation between expression of the selected MHC antigens in each grade of @entity3146 ( @entity3146 ) and invasive @entity189 . METHODS: Quantitative comparative analysis of MHC class I and class II expression between specimens of the uterine cervix was carried out. For detecting human @entity4107 molecules, two primary antibodies were used, recognizing either epitopes of all free heavy chain class I molecules or epitopes of the HLA- @entity13620 antigen. HLA class II antigens were detected by antibodies reacting with the @entity7722 of the HLA-DR molecule. An indirect immunoperoxidase technique was carried out using paraffin-embedded sections. The following groups of sections were formed: squamous metaplasia, @entity3146 of different grades, and @entity189 . The specimens were taken from @entity1 in whom routine histopathologic diagnosis of @entity75 had been performed. RESULTS: A significant decrease in the mean number of stained cells showing expression of MHC class I antigens ( @entity29152 and @entity13620 ) was found in the invasive @entity5 group of specimens. A decrease in @entity13620 expression was also found in the @entity3146 group. The highest expression of the HLA-DR antigens was detected in the metaplastic epithelium compared to the other groups. In the @entity6349 epithelium the expression of the HLA-DR antigens was increased in the highest grade of @entity1141 ( @entity3146 ). Upregulation of HLA-DR expression was detected in invasive @entity5 specimens. CONCLUSIONS: Alterations in MHC expression are found in @entity75 and @entity957 of the uterine cervix. Quantitative assessment with the use of an image analysis system is valuable in helping to discriminate staining patterns of HLA expression. Further studies are indicated to evaluate the prognostic value of the expression of selected MHC antigens.
