XXXX gene expression in @entity1 fetal lung airways.

@entity1 @entity11028 @entity3231 @entity825
In the present study, we characterized @entity3231 messenger RNA (mRNA) in mid-trimester @entity1 fetal @entity825 and bronchi. @entity3231 protein was localized by immunocytochemistry to scattered epithelial cells in the airway surface epithelium and in submucosal glands of the fetal @entity825 and bronchi. @entity3231 mRNA (2.2 kb) was detected by Northern blot analysis in @entity1 fetal @entity825 , as well as in primary and more distal bronchi. The levels of detectable @entity3231 mRNA were highest in the upper airways and were decreased in smaller bronchi in comparison. @entity3231 mRNA was barely detectable in the distal fetal lung tissue. In contrast, @entity3231 mRNA was abundant in cultured explants of distal @entity1 fetal lung tissue. @entity3231 and @entity11028 mRNA were detected by primer extension analysis in adult @entity1 lung tissue and in cultured @entity1 fetal lung explants. Only @entity11028 mRNA was detected in RNA isolated from @entity1 fetal @entity825 and bronchi. @entity3231 mRNA was localized by in situ hybridization in the fetal @entity825 and bronchi in scattered cells in the surface epithelium and, most prominently, in submucosal glands. Our results suggest that @entity11028 , and not @entity3231 , is produced in the @entity1 fetal tracheal and bronchial epithelium and in submucosal glands.
