[Establishment of a cell line from XXXX @entity43 ( @entity189 of FIGO classification) and analysis of its cell-biological characteristics and sensitivity to anticancer drugs].

@entity43 @entity1 @entity632 @entity19 @entity5 @entity75 @entity900 @entity189 @entity2879 @entity1891 @entity1487
A new @entity43 cell line has been established from the dispersed cells taken from a heterotransplanted @entity5 at the 6th passage in @entity19 which had been serially transplanted with a @entity75 of @entity189 (FIGO) of the ovary. Selection of cancerous epithelioid cells was completed by the 4th passage by means of the colonial cloning method. The cell line designated TYK-nu was subcultured serially in Eagle's MEM with 10% FCS more than 100 times over 31 months at March, 1986 and was observed to produce a @entity5 on implantation into @entity19 histologically similar to the @entity5 . These cells form a monolayer in a pavement-like arrangement with polygonal cells including giant cells and reveal a lack of contact inhibition. PAS positive substance can be seen in the cytoplasm. Desmosome structure, well-developed cell organelles and glycogen granules can be found by electron microscopy. The population doubling time was about 37 hours and the plating efficiency was 13-17%. All cells were seen to be of the @entity1 karyotype on chromosomal analysis and the number of chromosome in the majority of cells was hyperdiploidy with a mode of 56 at the 6th and 84th passages. A submetacentric marker chromosome was present. Using in vitro colony assay, TYK-nu was studied for drug sensitivity to @entity900 , @entity632 , @entity1891 , @entity1487 and @entity2879 , and found to be sensitive to @entity900 .
