XXXX lipopolysaccharide alters atherosclerotic-related gene expression in oxidized low-density-lipoprotein-induced macrophages and foam cells.

@entity1 @entity1357 @entity281 @entity731 @entity65 @entity2523
BACKGROUND AND OBJECTIVE: The molecular mechanism linking @entity731 formation and periodontal pathogens is not clear, although a positive correlation between periodontal @entity281 and @entity65 has been reported. The aim of this study was to determine whether stimulation with @entity2523 lipopolysaccharide (LPS) affected the expression of @entity731 -related genes, during and after the formation of foam cells. MATERIAL AND METHODS: Macrophages from @entity1 @entity1357 monocytes were treated with oxidized low-density lipoprotein (oxLDL) to induce the formation of foam cells. @entity2523 LPS was added to cultures of either oxLDL-induced macrophages or foam cells. The expression of @entity731 -related genes was assayed by quantitative real-time PCR, and the production of granulocyte-macrophage colony-stimulating factor, monocyte chemotactic protein-1, interleukin (IL)-1b, IL-10 and IL-12 proteins was determined using ELISA. Nuclear translocation of nuclear factor-kappaB (NF-kB) P(65) was detected by immunocytochemistry, and western blotting was used to evaluate inhibitory kappa B-a (Ik  -a) degradation to confirm activation of the NF-kB pathway. RESULTS: @entity2523 LPS stimulated @entity731 -related gene expression in foam cells and increased the oxLDL-induced expression of chemokines, adhesion molecules, growth factors, apoptotic genes and nuclear receptors in macrophages. Transcription of the proinflammatory cytokines IL1b and IL12 was elevated in response to LPS in both macrophages and foam cells, whereas transcription of the anti-inflammatory cytokine, IL10, was not affected. Increased activation of the NF-kB pathway was also observed in macrophages costimulated with LPS + oxLDL. CONCLUSION: @entity2523 LPS appears to be an important factor in the development of @entity731 by stimulation of @entity731 -related gene expression in both macrophages and foam cells via activation of the NF-kB pathway.
