[Role of hepatic stellate cells in the angiogenesis of XXXX ].

@entity1 @entity7 @entity5 @entity665 @entity540 @entity157 @entity687 @entity99
BACKGROUND/AIMS: Hepatic stellate cells (HSCs) in the @entity157 are responsible for @entity5 encapsulation as a host defense mechanism. Recently, it was suggested that HSCs might play an important role in hepatic angiogenesis. Thus, HSCs in the HCC may be involved in @entity5 angiogenesis and pathogenesis of @entity99 . The purpose of this study was to examine the involvement of activated HSCs in the angiogenesis of @entity157 . METHODS: We investigated the effect of @entity1 HSC conditioned medium (CM) on the endothelial cell proliferation with or without stimulation of HepG2 CM, using @entity665 incorporation assay. Additionally, we investigated the effect of HepG2 CM on HSCs proliferation and messenger RNA (mRNA) expression of various pro-angiogenic factors such as @entity687 ( @entity687 ), @entity540 ( @entity540 ), and @entity7 ( @entity7 ) in HSCs. RESULTS: HSC CM caused a significant increase in DNA synthesis in @entity1 umbilical vein endothelial cells (HUVEC). The endothelial proliferation effect of HSCs was augmented by HepG2 CM. HepG2 CM significantly increased HSCs proliferation and stimulated @entity687 and @entity540 mRNA expression in HSCs. CONCLUSIONS: HSCs promote endothelial proliferation through various soluble factors. The soluble factors secreted in HepG2 stimulate HSC proliferation and up-regulate mRNA expression of proangiogenic factors. This result suggests that HSCs may play an important role in the angiogenesis of @entity157 .
