Heparin treatment increases thioredoxin interacting protein expression in XXXX cells.

@entity6 @entity1735 @entity6950 @entity35 @entity157 @entity413
UNASSIGNED: Heparins play an important role in cell growth, differentiation, migration and invasion. However, the molecular mechanisms of heparin mediated cellular behaviors are not well defined. To determine the effect of heparin on gene expression, we performed a cDNA microarray in a @entity157 cell line and found that heparin regulates transcription of genes involved in @entity413 metabolism. In this study, we showed a new role of heparin in the regulation of thioredoxin interacting protein, which is a major regulator of @entity413 metabolism, in @entity157 cell lines. We determined the importance of a unique @entity1735 response element located on its promoter for the @entity6950 -induced activation of thioredoxin-interacting protein and the modulatory role of heparin on nuclear accumulation of @entity1735 response element associated proteins. We showed the importance of heparin mediated histone modifications and down-regulation of Enhancer of zeste 2 polycomb repressive complex 2 expression for heparin mediated overexpression of thioredoxin-interacting protein. When we tested biological significance of these data; we observed that cells overexpressing thioredoxin-interacting protein are less adhesive and proliferative, however they have a higher migration and invasion ability. Interestingly, heparin treatment increased thioredoxin-interacting protein expression in liver of @entity6 @entity35 . In conclusion, our results show that heparin activates thioredoxin-interacting protein expression in liver and @entity157 cells and provide the first evidences of regulatory roles of heparin on @entity1735 response element associated factors. This study will contribute future understanding of the effect of heparin on @entity413 metabolism and @entity413 independent overexpression of thioredoxin-interacting protein during hepatocarcinogenesis.
