Skewing of cytotoxic activity and chemokine production, but not of XXXX expression, in @entity1 type-1/-2 gamma delta T lymphocytes.

@entity1 @entity23427 @entity32 @entity366 @entity23159 @entity5075 @entity12868 @entity14420 @entity5823 @entity16952 @entity379 @entity34486 @entity3646 @entity687 @entity688 @entity436
@entity1 Vgamma9/Vdelta2(+) T lymphocytes participate in the immune response against intracellular pathogens through the secretion of type-1 cytokines and chemokines and by killing of infected cells. Little is known of the effects by type-2 differentiation of gamma delta cells on these functions. Here, we report that bona fide naive cord blood-derived gamma delta lymphocytes expanded in vitro with the mycobacterial antigen @entity23159 ( @entity34486 ) can be differentiated as either type-1 or type-2 cells, in the presence of an appropriate cytokine milieu. Instead, peripheral gamma delta cells from PPD-negative healthy adults displayed a type-1 cytokine profile, i.e. IPP-stimulated secretion of @entity379 , but not of @entity3646 and @entity688 . Moreover, they released the @entity12868 , but not @entity687 nor the Th2 chemoattractants I-309 and @entity14420 ( @entity14420 ). This cytokine profile was not significantly affected by in vitro culture in Th2 polarizing conditions. Only in one case out of seven were peripheral gamma delta cells fully differentiated to type-2 lymphocytes, characterized by sustained @entity3646 and @entity688 production, along with secretion of substantial amounts of @entity687 , I-309 and @entity14420 . Type-2 gamma delta T lymphocytes preferentially expressed the co-stimulatory molecule @entity5823 ; conversely, no skewing in @entity436 expression was observed. Both polarized populations displayed high levels of @entity366 in the absence of @entity23427 , @entity16952 and @entity5075 . Finally, type-1, but not type-2, gamma delta T lymphocytes killed IPP-pulsed U937 cells and displayed elevated perforin content. Overall, our data suggest that type-2 differentiation of gamma delta T lymphocytes profoundly affects both their effector functions and their potential to recruit the appropriate leukocyte subsets to the sites of @entity32 .
