Apoptosis of cultured granulosa-lutein cells is reduced by XXXX and may correlate with @entity1949 and pregnancy rate.

@entity1 @entity1949 @entity2690 @entity442
OBJECTIVE: To correlate apoptosis of cultured @entity1 granulosa-lutein cells (GL cells) with the outcome of IVF ( @entity1949 and pregnancy rate) and to study the effect of @entity2690 ( @entity442 ) on apoptosis. DESIGN: In vitro assays. SETTING: University laboratory and private IVF center. @entity1 (S): Eighty-one @entity1 undergoing IVF. INTERVENTION(S): Purified @entity1 GL cells from pooled follicles were cultured for 48 hours in serum-free media with or without @entity2690 and @entity442 . Cumulus cells and mural GL cells were evaluated separately. MAIN OUTCOME MEASURE(S): Detection of apoptosis by using caspACE FITC-VAD-FMK, a fluorescent in situ marker for activated caspases; @entity1949 ; and pregnancy. RESULT(S): Age younger than 38 years and successful pregnancy were associated with less apoptosis (33.0% +/- 17.2% vs. 43.2% +/- 18.0% and 30.2% +/- 14.0% vs. 40.4% +/- 19.5%, respectively). There was a linear correlation between @entity1949 and GL cell apoptosis. @entity442 decreased apoptosis in a dose-dependent fashion. A statistically significant effect (17% decrease) was reached at a dose of 10 nM. @entity2690 (10 nM) caused a small (8%) decrease in apoptosis, but this effect did not reach statistical significance. Cumulus cells consistently had <3% apoptosis. CONCLUSION(S): [1] Apoptosis of cultured GL cells may be associated with IVF outcome and ovarian reserve and [2] @entity442 decreases apoptosis of cultured GL cells.
