Irradiation-induced localization of IL-12-expressing mesenchymal stem cells to enhance the curative effect in @entity19 metastatic XXXX .

@entity137 @entity1750 @entity5 @entity19 @entity3 @entity157
UNASSIGNED: Irradiation in conjunction with gene therapy is considered for efficient @entity5 treatment. Mesenchymal stem cells (MSCs), due to their irradiation-promotable @entity5 tropism, are ideal delivery vehicles for gene therapy. In this study, we investigated whether treatment with radiation and interleukin (IL)-12-expressing MSCs (MSCs/IL-12) exerts improved antitumor effects on @entity19 metastatic @entity157 . HCa-I and Hepa 1-6 cells were utilized to generate heterotopic @entity19 @entity157 models. Tumor-bearing @entity19 were treated with irradiation or MSCs/IL-12 alone, or a combination. @entity1750 ( @entity1750 / @entity1750 ) expression was assessed in irradiated @entity157 tissues to confirm a chemotactic effect. Combination treatment strategies were established and their therapeutic efficacies were evaluated by monitoring @entity5 growth, @entity3 and survival rate. IL-12 expression was assessed and the apoptotic activity and immunological alterations in the @entity5 microenvironment were examined. @entity1750 / @entity1750 expression and localization of MSCs/IL-12 increased in the irradiated @entity19 @entity157 cells. The antitumor effects, including suppression of pulmonary @entity3 and survival rate improvements, were increased by the combination treatment with irradiation and MSCs/IL-12. IL-12 expression was increased in @entity5 cells, causing proliferation of cluster of differentiation 8(+) T-lymphocytes and natural killer cells. The apoptotic activity increased, indicating that the @entity137 of immune cells was involved in the antitumor effect of the combined treatment. Treatment with irradiation and MSCs/IL-12 showed effectiveness in treating @entity19 metastatic @entity157 . IL-12-induced proliferation of immune cells played an important role in apoptosis of @entity5 cells. Our results suggest that treatment with irradiation and MSCs/IL-12 may be a useful strategy for enhancing antitumor activity in metastatic @entity157 .
