[Effect of bevacizumab on proliferation and invasion of XXXX @entity2 A549 cells].

@entity1 @entity1476 @entity2 @entity1916 @entity1495
OBJECTIVE: To study the effect and mechanism of bevacizumab on proliferation and invasion of @entity1 @entity2 A549 cells. METHODS: A549 cells were treated with bevacizumab. Proliferation and invasion of the bevacizumab-treated A549 cells were detected using cell counting kit CCK-8 and Transwell assay, respectively. The expression of the mRNA and protein of @entity1476 , @entity1495 and @entity1916 were detected by real-time PCR and Western blotting, respectively. RESULTS: Proliferation activity was inhibited at the concentration of 10   g/ml and promoted at the concentration of 100   g/ml bevacizumab. Bevacizumab in the concentration of 50   g/ml had a stronger inhibitory effect on the invasion of A549 cells (16 406.19  5 674.23 penetrated cells) than that of control group (36 108.68  6 263.83, P<0.05). The real-time PCR showed that bevacizumab had a stronger inhibitory effect on the expression of @entity1476 and @entity1495 mRNA at the concentration of 50   g/ml and on the expression @entity1916 mRNA at the concentration of 10   g/ml bevacizumabin the A549 cells. However bevacizumab at the concentration of 100   g/ml showed a promoting effect on the expression of @entity1476 , @entity1495 and @entity1916 mRNA (1.82  0.31, 1.60  0.25, 2.63  0.48), significantly higher than that of the control group (1.00  0.19, 1.00  0.23, 1.00  0.22, P<0.05). The expression of @entity1476 , @entity1495 and @entity1916 mRNA and protein was inhibited by 10   g/ml bevacizumab in a time-dependent manner. The Western blot assay showed that bevacizumab had a bi-directional effect on the expression of @entity1476 and @entity1916 proteins in the A549 cells: a promoting effect at 100   g/ml and inhibitory effect on the expression of @entity1476 at 50   g/ml bevacizumab, and inhibitory effect on the expression of @entity1916 protein at 10   g/ml bevacizumab. CONCLUSIONS: Our findings indicate that in a certain range of concentrations, bevacizumab has prominent inhibitory effect on the proliferation and invasion of A549 cells. However,over the concentration of 100   g/ml, bevacizumab shows a weakening anti-invasion effect, even has a promoting effect on cell proliferation. This phenomenon may be related to the inhibiting effect on the expression of @entity1476 and @entity1916 proteins in a non-concentration-dependent manner by bevacizumab.
