[The establishment of a XXXX pancreatic mixed @entity513 cell line].

@entity1 @entity788 @entity19 @entity5 @entity984 @entity513
OBJECTIVE: Establishment of a @entity1 pancreatic mixed @entity513 cell line. METHODS: A specimen of this type of @entity5 was obtained from a male @entity1 at the head of pancreas. The @entity5 tissue was minced into pieces and inoculated subcutaneously in @entity19 . With two successive subcutaneous implants, a piece of @entity5 tissue taken from the 2(nd) implanted @entity5 of a nude @entity19 was minced into minute tissue masses and inoculated in a media containing 10% fetal bovine serum. RESULTS: Currently, it has been passed to the 55(th) passage. Cells of this cell line (PC-EN) kept simultaneously the morphologic patterns of a @entity788 which were verified by histochemistry and immunohistochemistry assays. Some of the PC-EN cells showed presence of neuroendocrine granules by electron microscopy. The growth rate of this cell line was relatively low. Kakryotype analysis showed two cell populations with its major mode of chromosomes numbers as 48 in one, and 56 in the other. Study on @entity984 gene demonstrated that PC-EN harbored a mutated codon 12. Several gastrointestinal hormones and CEA were detected in PC-EN cells and in the supra-cultured medium using radioimmunoassay. CONCLUSIONS: The cellular and molecular biologic characteristics of PC-EN cells illustrated that this cell line preserved the morphology of a @entity788 and possessed a special growth property, chromosome karyotype, and mutation of @entity984 gene, but also showed a biologic exocrine and endocrine functions of the pancreas. The establishment of PC-EN cell line will provide a useful model in vitro for further investigating the cellular and molecular biology of exocrine and endocrine @entity5 of the pancreas.
