Apocynum Venetum Leaf Attenuates XXXX /Reperfusion Injury by Inhibiting Oxidative Stress.

@entity2508 @entity63 @entity4286 @entity1188 @entity741 @entity35
UNASSIGNED: Apocynum venetum, a Chinese medicinal herb, is reported to be neuroprotective. However, whether apocynum venetum leaf extract (AVLE) protects against @entity63 remains elusive. Our present study was aimed to observe the effects of AVLE preconditioning on @entity741 /reperfusion (MI/R) injury and to investigate the possible mechanisms. @entity35 were treated with AVLE (500 mg/kg/d, o.g.) or distilled water once daily for one week. Afterward, all the animals were subjected to 30 min of @entity741 followed by 4 h of reperfusion. AVLE preconditioning for one week significantly improved cardiac function following MI/R. Meanwhile, AVLE reduced @entity1188 size, plasma creatine kinase (CK)/lactate dehydrogenase (LDH) activities and myocardial apoptosis at the end of reperfusion in @entity35 hearts. Moreover, AVLE preconditioning significantly inhibited superoxide generation, gp91(phox) expression, malonaldialdehyde formation and enhanced superoxide dismutase (SOD) activity in I/R hearts. Furthermore, AVLE treatment increased Akt and extracellular regulated protein kinases 1/2 ( @entity2508 ) phosphorylations in I/R @entity35 heart. Either the Phosphatidylinositide 3-kinase (PI3K) inhibitor wortmannin or the @entity2508 inhibitor @entity4286 blocked AVLE-stimulated anti-oxidative effects and cardioprotection. Our study demonstrated for the first time that AVLE reduces oxidative stress and exerts cardioprotection against MI/R injury in @entity35 .
