Milk-induced XXXX is associated with the expansion of T cells expressing cutaneous lymphocyte antigen.

@entity1 @entity32 @entity483 @entity6132 @entity5132 @entity1196 @entity56 @entity272 @entity1964 @entity14761 @entity484
The extravasation of T cells at sites of @entity32 is critically dependent on the activity of homing receptors (HR) involved in endothelial cell recognition and binding. Two such HR (the cutaneous lymphocyte antigen [CLA] and @entity1196 ) have been shown to be selectively involved in T cell migration to skin and peripheral lymph nodes, respectively. This study was designed to assess the relationship between the organ specificity of an @entity56 to food and the expression of HR on T cells activated in vitro by the relevant food allergen. Peripheral blood mononuclear cells were isolated from seven milk @entity56 @entity1 with a history of @entity484 when exposed to milk. All @entity1 had a positive prick skin test and double-blind placebo-controlled food challenge to milk. 10 @entity1 with either @entity56 @entity14761 or milk-induced @entity1964 and 8 nonatopic adults served as controls. Five-parameter flow cytometry using monoclonal antibodies was used for detection of the specific HR on freshly isolated T cells versus T cell blasts induced by a 6-d incubation with casein, as compared with @entity5132 . After in vitro stimulation with casein, but not @entity5132 , @entity1 with @entity6132 and @entity483 had a significantly greater percentage of CLA+ T cells (P < 0.01) than controls with milk-induced @entity1964 , @entity56 @entity14761 , or nonatopic healthy controls. In contrast, the percentage of @entity1196 -expressing T cells did not differ significantly between these groups. These data suggest that after casein stimulation @entity56 @entity1 with milk-induced @entity272 have an expanded population of CLA+ T cells, as compared with nonatopics or @entity56 @entity1 without skin involvement. We postulate that heterogeneity in the regulation of HR expression on antigen-specific T cells may play a role in determining sites of involvement in tissue-directed @entity56 responses.
