Bone formation by @entity35 bone marrow cells cultured on XXXX fiber mesh: effect of in vitro culture time.

@entity78 @entity460 @entity35 @entity702 @entity4653
The objective of this study was to examine the effect of cell culture time on bone formation by @entity35 bone marrow cells seeded in @entity702 fiber mesh. As a seeding technique, a high cell suspension was used (3 x 10(6) cells/mL). Therefore, 30 meshes were repeatedly rotated in @entity35 mL tube (containing 30 x 10(6) cells) on a rotation plate (2 rpm) for 3 h. Osteogenic cells were cultured for 1, 4, and 8 days on @entity702 fiber mesh and finally implanted subcutaneously in @entity35 . Meshes without cells were also implanted subcutaneously in @entity35 . DNA and @entity4653 ( @entity4653 ) analyses and @entity460 measurements determined cellular proliferation and differentiation during the in vitro incubation period of the mesh implants. Four weeks after implant insertion, the animals were sacrificed. The implants, with their surrounding tissue, were retrieved and prepared for histologic evaluation and @entity460 measurements. DNA analysis of the in vitro experiment showed a lag phase from day 1 through day 4, but a 42% increase in DNA between days 4 and 8. @entity4653 and @entity460 measurements indicated an increase in @entity460 from day 1 to day 4, yet only a small but significant increase from days 4 to 8. Histologic analysis demonstrated that bone was formed in all day 1 and day 4 implants, and that the bone-like tissue was present uniformly through the meshes. The bony tissue was morphologically characterized by osteocytes embedded in a mineralized matrix, with a layer of osteoid and osteoblasts at the surface. The day 8 implants showed only @entity78 deposition in the @entity702 fiber mesh. @entity460 measurements of the implants revealed that calcification in day 1 implants was significantly higher (p < 0.05) compared to day 4 and day 8 implants. No significant difference in @entity460 content existed between day 4 and day 8 implants. On the basis of our results, we conclude that 1) bone formation was generated more effectively in osteogenic cells by a short culture time after seeding in @entity702 fiber mesh; 2) dynamic cell seeding is probably more effective than static cell seeding; and 3) selection of the right cells from the heterogenous bone marrow population remains a problem.
