Modifying enzymes related @entity5292 : analyses of resistant XXXX isolates.

@entity801 @entity2892 @entity2889 @entity5292 @entity581 @entity3064
Enzymatic modification of @entity581 by nucleotidyltransferases, acetyltransferases and/or phosphotransferases accounts for the majority of @entity5292 -resistant @entity3064 isolates. In this study, we investigated the relationship between @entity5292 resistance and the presence of @entity5292 -modifying enzymes in @entity3064 clinical isolate groups with different resistance profiles. Thirty-two clinical A. baumannii isolates were included in this study. @entity3064 isolates were divided into 4 groups according to results of susceptibility testing. The presence of genes encoding the following @entity5292 -modifying enzymes; aph (3')-V1, aph (3')-Ia, aac (3)-Ia, aac (3) IIa, aac (6')-Ih, aac (6')-Ib and ant (2')-Ia responsible for resistance was investigated by PCR in all strains. The acetyltransferase (aac (6')-Ib, aac (3)-Ia) and phosphotransferase (aph (3')-Ia) gene regions were identified in the first group, which comprised nine @entity2892 , @entity2889 , and @entity801 -resistant isolates. The acetyltransferase (aac (6')-Ib, aac (3)-Ia), phosphotransferase (aph (3')-VI) and nucleotidyltransferase (ant2-Ia) gene regions were identified in the second group, which was composed of nine @entity2892 -resistant, @entity2889 -resistant and @entity801 -sensitive isolates. The acetyltransferase (aac (3)-Ia) and phosphotransferase (aph (3')-Ia) regions were identified in the fourth group, which comprised eight @entity2892 -sensitive, @entity2889 -sensitive and @entity801 -resistant isolates. Modifying enzyme gene regions were not detected in the third group, which was composed of six @entity2892 , @entity2889 and @entity801 -sensitive isolates. Our data are consistent with previous reports, with the exception of four isolates. Both acetyltransferases and phosphotransferases were widespread in A. baumannii clinical isolates in our study. However, the presence of the enzyme alone is insufficient to explain the resistance rates. Therefore, the association between the development of resistance and the presence of the enzyme and other components should be investigated further.
