Comparison of structural genetics of non-schistosoma-associated XXXX .

@entity71244 @entity986 @entity5 @entity957 @entity189 @entity150
Little is known about genetic changes in squamous differentiation of non-schistosomiasis-associated @entity150 . Therefore, we investigated pure @entity957 ( @entity957 ), squamous parts of mixed @entity189 with squamous differentiation (MIX) and mere @entity5 ( @entity5 ) for structural genetic differences. Tissue microarray slides (n = 29 @entity957 , n = 35 MIX and n = 23 @entity5 ) were analyzed by ZytoLight SPEC @entity986 / @entity71244 /7/17 Quadruple Color Probe fluorescence-in-situ-hybridization (FISH) and DNA was investigated by comparative genomic hybridization (CGH) (n = 35 SqCCs, n = 40 MIX and n = 36 @entity5 ). By FISH the mean number of polysomic cells was lowest in @entity957 ( @entity71244 P = 0.0498, CEN17 P = 0.0009). A slight tendency of lower copy numbers of chromosomes 3, 7 and 17 and higher numbers of the @entity986 -locus in @entity957 (P = 0.45) indicated less @entity5 cells in @entity957 compared to MIX and @entity5 . In CGH @entity957 showed the lowest mean number of aberrations per @entity5 ( @entity957 5.37 changes, MIX 6.75 and @entity5 7.64; P = 0.1754). Significant differences between the three groups were found for loss of chromosome 3p (P = 0.004), 6q (P = 0.028), 11p (P = 0.024) and gains of 5p (P = 0.020). Loss of 3p was more frequent in @entity957 (51.4%) than in MIX (37.5%) or @entity5 (13.9%). To conclude, SqCCs show less polysomy and genetic alterations than MIX and @entity5 . Loss of 3p is more frequent in @entity957 but there are no absolute specific alterations for each @entity5 group. Squamous parts of @entity5 show similar alterations than @entity5 and should be considered as further development of @entity5 , while pure @entity957 seem to be a separate @entity5 group.
