Histone deacetylation of memory T lymphocytes by You-Gui-Wan alleviates allergen-induced @entity32 in XXXX .

@entity32 @entity59 @entity19986 @entity19 @entity565 @entity12231 @entity61 @entity71131 @entity701 @entity58 @entity1312 @entity19985 @entity71132 @entity45091 @entity630 @entity68775
BACKGROUND: You-Gui pills (You-Gui-Wan; YGW) can promote T lymphocyte proliferation and differentiation, and restore @entity630 / @entity61 balance in the treatment of @entity565 , but their mechanism of action is not fully known. This study aims to explore whether YGW can induce histone deacetylation or acetylation in memory T lymphocytes (Tm) for improvement of airway @entity32 in @entity565 . METHODS: CD4(+)CD45RB(low) cells, as Tm, were obtained by magnetic-activated cell sorting and flow cytometry from the spleens of BALB/c @entity19 with ovalbumin (OVA)-induced @entity565 . Tm were cocultured with @entity701 (CORT; 1000 nM), serum containing low (0.225  g/kg), moderate (0.9  g/kg), or high (3.6  g/kg) doses of YGW, or medium only, and then adoptively transferred into na  ve @entity19 (n   =   5 per group). Recipient @entity19 were challenged with aerosolized OVA. The levels of @entity58 , @entity59 , @entity1312 , and IFN-y in culture supernatants and bronchoalveolar lavage fluid (BALF) from the OVA-challenged @entity19 were measured by ELISA. Histone deacetylase (HDAC) and @entity45091 ( @entity45091 ) activities and protein expressions of @entity19985 , @entity19986 , and @entity12231 -11 in lung tissue were measured by western blotting analyses. The alveolar eosinophilic @entity32 index (AEII) was evaluated in the lungs of adoptive transfer recipient @entity19 . RESULTS: YGW reduced @entity32 and eosinophil infiltration into the lung tissues as evidenced by histology, with similar effects to those of CORT. High-, moderate-, and low-YGW increased HDAC (P   <   0.0001, P   =   0.0009 and P   =   0.0253 respectively) and decreased @entity45091 (P   =   0.0001, P   =   0.0000 and P   =   0.0039, respectively) activities in dose-dependent manners in the lung tissues of adoptive transfer recipient @entity19 . Increased histone deacetylation of Tm by YGW reduced the AEII by reducing @entity19986 (P   =   0.014), @entity58 (P   =   0.0004), @entity59 (P   =   0.0067), and @entity1312 (P   =   0.0002), and inducing IFN-y release (P   =   0.0375). Moreover, YGW reduced inflammatory cytokines such as @entity58 , @entity59 , and @entity1312 by upregulating the activities of @entity71131 (P   =   0.003)/10 (P   =   0.003), @entity68775 (P   <   0.0001), and @entity71132 -11 (P   <   0.0001, P   <   0.0001 and P   <   0.0001, respectively), respectively, and increased IFN-y release by increasing @entity71132 (P   <   0.0001). CONCLUSIONS: Histone deacetylation of Tm was observed during alleviation of allergen-induced @entity32 in @entity565 by YGW.
