Downregulation of miR-95-3p inhibits proliferation, and invasion promoting apoptosis of @entity11 cells by targeting XXXX .

@entity1 @entity29726 @entity5 @entity67396 @entity11 @entity98 @entity71403 @entity36119
@entity11 are the most common and aggressive types of @entity5 in @entity1 brain, of which the prognosis remains dismal because of their biological behavior. The involvement of miRNAs in tumorigenesis of various kinds of @entity5 drives us to explore new miRNAs related to @entity11 . We measured expression level of @entity36119  -3p by qRT-PCR in @entity1 @entity11 and @entity5 brain tissues and found that higher level of @entity36119  -3p in @entity11 tissues of higher grade. Biological functions of @entity36119  -3p on @entity11 cells were investigated by @entity98 assay, flow cytometry and transwell assay. We discovered the cell lines transfected with @entity36119  -3p ASO (antisense oligonucleotide) had retarded proliferation and invasion but enhanced apoptosis ability. We searched on-line tool Targetscan and selected @entity29726 ( @entity71403 ) as a putative target. Luciferase reporter was employed to confirm the binding sites in 3'UTR region of @entity67396 for @entity36119  -3p. The correlation between expression of @entity67396 and @entity36119  -3p was determined by western blotting and qRT-PCR both in cell lines and @entity1 samples. Results showed @entity67396 was a direct target of @entity36119  -3p and expression levels of @entity67396 and @entity36119  -3p were negatively correlated. Finally, @entity67396 largely abrogated the effects of @entity36119  -3p on proliferation, invasion and apoptosis of @entity11 cells in rescue experiments, which verified the role of @entity67396 in @entity36119  -3p regulating @entity11 biological behavior. In conclusion, our data suggest the expression level of @entity36119  -3p is positively related to @entity11 grade and downregulation of @entity36119  -3p affects proliferation, invasion and apoptosis of @entity11 cells by targeting @entity67396 . We identified @entity36119  -3p as a putative therapeutic target and @entity67396 as a potential @entity5 suppressor.
