@entity5 suppressor role of microRNA-1296 in triple-negative XXXX .

@entity0 @entity59102 @entity146 @entity5 @entity900 @entity4910
UNASSIGNED: @entity0 ( @entity0 ) is an aggressive subtype of @entity0 with a poor prognosis, which lacks effective targeted therapies. There is an urgent need to better understand the underlying molecular mechanisms of @entity0 @entity146 and identify novel, efficient targets for therapeutic intervention. METHODS: miRNA qRT-PCR was used to determine the expression of @entity59102 in cell lines. The @entity59102 overexpression effects in @entity0 cell lines were investigated using assays of colony formation, cell cycle and apoptosis. Immunoblotting was performed to determine the expression of the @entity59102 target protein, and luciferase assays were performed to confirm the target of @entity59102 action. RESULTS: @entity59102 expression was significantly suppressed in @entity0 cell lines and tissues samples. Overexpression of @entity59102 significantly suppressed cell proliferation of two @entity0 cell lines when compared to control miRNA-expressing cells. A significant decrease in the S-phase of the cell cycle was observed following @entity59102 overexpression, accompanied by induction of apoptosis in @entity0 cells. @entity4910 ( @entity4910 ) was identified as a target of @entity59102 action. @entity59102 overexpression significantly suppressed the luciferase activity of reporter plasmid containing the 3'UTR of @entity4910 and protein expression levels of @entity4910 in @entity0 cells. The effects of @entity59102 overexpression on @entity0 cell growth were reversed by @entity4910 overexpression. @entity59102 expression sensitized @entity0 cells to @entity900 treatment. CONCLUSION: Our results demonstrate a novel @entity5 suppressor role for @entity59102 in triple-negative @entity0 cell lines, identify @entity4910 as its target of action, and demonstrate a potential role for @entity59102 in sensitizing @entity0 cells to @entity900 .
