Altered expression of neuroplasticity-related genes in the brain of XXXX suicides.

@entity1 @entity16167 @entity308 @entity41234 @entity40154 @entity50329 @entity460 @entity28916
BACKGROUND: Expression of the neuronal membrane glycoprotein M6a ( @entity41234 ), the proteolipid protein ( @entity16167 /DM20) family member, is downregulated in the hippocampus of chronically stressed animals. Its neuroplastic function involves a role in neurite formation, filopodium outgrowth and synaptogenesis through an unknown mechanism. Disruptions in neuroplasticity mechanisms have been shown to play a significant part in the etiology of @entity308 . Thus, the current investigation examined whether @entity41234 expression is also altered in @entity1 @entity308 brain. METHODS: Expression levels and coexpression patterns of @entity41234 , @entity28916 , and @entity16167 (two other members of @entity16167 /DM20 family) as well as of the neuroplasticity-related genes identified to associate with @entity41234 were determined using quantitative polymerase chain reaction (qPCR) in postmortem samples from the hippocampus (n=18) and the prefrontal cortex (PFC) (n=25) of @entity308 suicide victims and compared with control subjects (hippocampus n=18; PFC n=25). Neuroplasticity-related proteins that form complexes with @entity41234 were identified by coimmunoprecipitation technique followed by mass spectrometry. RESULTS: Results indicated transcriptional downregulation of @entity41234 and @entity28916 in the hippocampus of @entity308 suicides. The expression level of @entity460 /calmodulin-dependent protein kinase II alpha ( @entity40154 ) and @entity50329 ( @entity50329 ) was also significantly decreased. Subsequent analysis of coexpression patterns demonstrated coordinated gene expression in the hippocampus and in the PFC indicating that the function of these genes might be coregulated in the @entity1 brain. However, in the brain of @entity308 suicides this coordinated response was disrupted. CONCLUSIONS: Disruption of coordinated gene expression as well as abnormalities in @entity41234 and @entity28916 expression and expression of the components of @entity41234 complexes were detected in the brain of @entity308 suicides.
