Isolation and characterization of a protective antigen for adjuvant-free immunization against XXXX .

@entity738 @entity19 @entity383 @entity56 @entity380
A single, 68,000 m.w. glycoprotein antigen from adult @entity380 was purified by immunoaffinity chromatography with the use of a newly developed, protective, anti-schistosome murine monoclonal antibody. Immunization with two doses of 0.5 microgram or 1 microgram of purified antigen, without adjuvants, afforded a mean 28% reduction in parasite recovery in CF1 @entity19 , and 2-% reduction in parasite BALB/c @entity19 . On immunoblotting, the 68,000 m.w. antigen was common to @entity383 adults and schistosomula, whereas parasite eggs contained only cross-reacting low m.w. antigens of 19,100 and 16,000. Immunization resulted in the development of anti-antigen antibody and enhanced immediate cutaneous @entity56 to the 31-3B6 antigen. By contrast, @entity56 and sensitization to circumoval @entity738 formation were not observed in immunized @entity19 . It was concluded that the 68,000 m.w. 31-3B6 antigen represents a candidate vaccine for adjuvant-free immunization against @entity383 .
