Nanochelating based nanocomplex, GFc7, improves quality and quantity of XXXX mesenchymal stem cells during in vitro expansion.

@entity1 @entity3308 @entity920 @entity68
INTRODUCTION: @entity1 mesenchymal stem cells (hMSCs) have been approved for therapeutic applications. Despite the advances in this field, in vitro approaches are still required to improve the essential indices that would pave the way to a bright horizon for an efficient transplantation in the future. Nanotechnology could help to improve these approaches. Studies signified the important role of @entity68 in stem cell metabolism and efficiency of @entity920 chelation application for stem cell expansion   METHODS: For the first time, based on novel Nanochelating technology, we design an @entity68 containing @entity920 chelator nano complex, GFc7 and examined on hMSCs during in vitro expansion. In this study, the hMSCs were isolated, characterized and expanded in vitro in two media (with or without GFc7). Then proliferation, cell viability, cell cycle analysis, surface markers, HLADR, pluripotency genes expression, homing and antioxidative defense at genes and protein expression were investigated. Also we analyzed the spontaneous differentiation and examined osteogenic and lipogenic differentiation.   RESULTS: GFc7 affected the expression of key genes, improving both the @entity3308 and fitness of the cells in a precise and balanced manner. We observed significant increases in cell proliferation, enhanced expression of pluripotency genes and homing markers, improved antioxidative defense, repression of genes involved in spontaneous differentiation and exposing the hMSCs to differentiation medium indicated that pretreatment with GFc7 increased the quality and rate of differentiation. CONCLUSIONS: Thus, GFc7 appears to be a potential new supplement for cell culture medium for increasing the efficiency of transplantation.
