XXXX inhibits the proliferation of @entity1149 cells via upregulation of microRNA-7 expression.

@entity1 @entity588 @entity5 @entity1149 @entity2 @entity2823 @entity12333 @entity1205
@entity2 is the leading cause of @entity5 worldwide and about 85% of these are @entity1149 ( @entity1149 ). Several new chemotherapeutic agents have recently shown encouraging activity in @entity1149 , especially @entity1205 . MiRNAs (MicroRNAs) are closely related to @entity5 development. We studied miRNAs in @entity1149 cell lines to identify those that can regulate and predict the effectiveness of @entity1205 on @entity1149 . CCK8, Annexin and V- @entity2823 assays were carried out to evaluate the inhibitory effect of @entity1205 on @entity1149 cell linesA549 and H460, and qRT-PCR was used to detect and compare six miRNAs expression levels in the two cells with @entity1205 or not. Knockdown of @entity12333 by RNA interference and overexpression of @entity12333 were taken to evaluatethe effect of @entity12333 on @entity1205 effectiveness. Western blotting was used to evaluate the effect of @entity12333 on @entity588 in A549 and @entity1 cells. @entity1205 induced @entity1149 cell apoptosis and suppressed cell proliferation in vitro. @entity12333 expression levels were increased by @entity1205 in the two cell lines. @entity12333 overexpression improved anti-proliferative and pro-apoptotic effects of @entity1205 on the @entity1149 cells and that @entity12333 down-regulation decreased those effects. Moreover, subsequent experiments showed that @entity588 was downregulated by @entity12333 at both transcriptional and translational levels. This study further extends the biological role of @entity12333 in @entity1149 A549 and @entity1 cells and identifies @entity588 as a novel target possibly involved in @entity12333 -mediated growth suppression and apoptosis induction of @entity1149 cells.
