Production and activation of @entity1476 in XXXX .

@entity1 @entity1552 @entity5379 @entity3334 @entity6 @entity1476 @entity1551 @entity1495 @entity989
PURPOSE: To investigate the matrix metalloproteinase (MMP) species and their activation associated with the pathogenesis of @entity6 ( @entity6 ). METHODS: Sandwich enzyme immunoassays were used to measure concentrations of @entity3334 in vitreous samples from @entity1 with @entity6 and nondiabetic @entity989 . To evaluate activation ratios of the zymogen of @entity1476 (proMMP-2) and -9 (proMMP-9) in the vitreous samples and fibrovascular tissues, gelatin zymography was performed. Production and tissue localization of @entity1476 , @entity1551 ( @entity1551 ), @entity1552 , and @entity1495 in the fibrovascular tissues were examined by immunohistochemistry. mRNA expression of @entity1551 in the tissues was determined by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Among the seven different MMPs examined in the vitreous samples, only the levels of @entity5379 were significantly higher in the @entity6 samples than in the control. However, activation ratios of proMMP-2 (10.6% +/- 11.8%) and proMMP-9 (2.5% +/- 5.1%) in @entity6 vitreous samples were low and not significantly different from those of the control. In contrast, high activation ratios of proMMP-2 (54.3% +/- 13.6%) and notable activation of proMMP-9 (19.5% +/- 7.8%) were observed in the fibrovascular tissues. Immunohistochemical study demonstrated the localization of @entity5379 in the endothelial cells and glial cells of the fibrovascular tissues. @entity1476 was colocalized with @entity1551 and @entity1552 , which are an activator and an activation-enhancing factor, respectively, for proMMP-2. RT-PCR analysis indicated the gene expression of @entity1551 in the tissues. CONCLUSIONS: These data demonstrate that proMMP-2 is efficiently activated in the fibrovascular tissues of @entity6 , probably through interaction with @entity1551 and @entity1552 , and suggest the possibility that the activity of @entity1476 and @entity1551 is involved in the formation of the fibrovascular tissues.
