Compartment analysis of T cell receptor gamma--and immunoglobulin V(H) rearrangements by microdissection in patients with malt lymphoma and @entity102 with XXXX .

@entity6595 @entity1074 @entity1373 @entity102 @entity3621 @entity1534
The interpretation of clonality within @entity1534 -associated @entity3621 and low-grade @entity1074 remains controversial. Due to the observation of @entity1074 regression after @entity1534 eradication, new definitions concerning the border between benign reactive lesions and malignant @entity6595 are needed. Gene rearrangements for immunoglobulin heavy-chain in low-grade @entity1074 (N= 12) and @entity1534 -associated @entity102 with @entity1373 (N= 13) were analyzed by microdissection and polymerase chain reaction. Furthermore, T cell receptor-gamma chain rearrangements were analyzed by gene scan analysis. In 11 of 12 cases with initial low-grade @entity1074 , intraepithelial and subepithelial B cell rearrangements showed a restricted usage of the immunoglobulin heavy-chain 3. In @entity1534 -associated @entity102 , the intraepithelial B cell compartment showed an oligoclonal the immunoglobulin heavy-chain rearrangement pattern with a predominance of VH3. The subepithelial compartment did not show any restrictive immunoglobulin heavy-chain gene usage. Additionally a mono- to oligoclonal rearrangement pattern of the T cell receptor-y chain was observed in low-grade @entity1074 , whereas an oligoclonal pattem was observed in @entity102 . Our data provide evidence that low-grade @entity1074 may start within the epithelium and subsequently infiltrate the subepithelial compartment. The observation of a mono- to oligoclonal TCR-gamma rearrangement suggests that an antigen selecting process also takes place within reactive T cells. Combining TCR-gamma gene scan analysis with IgH chain rearrangement analysis might help in discriminating between @entity102 and @entity1074 in questionable cases.
