A combination of isolated phytochemicals and botanical extracts lowers diastolic blood pressure in a randomized controlled trial of XXXX subjects.

@entity1 @entity101 @entity3362 @entity3542 @entity1507 @entity2648 @entity6398 @entity842 @entity4044 @entity209 @entity4025 @entity3092
BACKGROUND/OBJECTIVES: Isolated phytochemicals have been shown to reduce blood pressure; however, combinations of phytochemicals have rarely been tested in @entity1 . We hypothesized that a combination of extracts from grape seed and skin (330   mg), green tea (100   mg), @entity4025 (60   mg) and a blend of @entity3092 , @entity4044 and bilberry (60   mg) would reduce blood pressure (BP) in @entity101 subjects. SUBJECTS/METHODS: Eighteen individuals meeting BP requirements (   130   mm   Hg systolic or    85   mm   Hg diastolic) and criteria for @entity209 were enrolled in a double-blinded, placebo-controlled, crossover trial (ClinicalTrials.gov, NCT01106170). The 28-day placebo and supplement arms were separated by a 2-week washout period, and 14   -h daytime ambulatory BP was assessed at baseline and at the end point of each arm. RESULTS: BP was not altered after placebo. After supplement treatment, diastolic pressure was reduced by 4.4   mm   Hg (P=0.024, 95% CI, 0.6-8.1), systolic pressure was unchanged and mean arterial pressure trended (P=0.052) toward reduction. Serum angiotensin-converting enzyme activity was similar between placebo and supplement arms, but urinary @entity1507 and @entity3542 concentrations were significantly increased (P=0.022) after supplementation. @entity1 aortic endothelial cells treated with metabolites of the @entity6398 used in the @entity1 supplement trial had a significant increase (P=0.005) in insulin-stimulated eNOS phosphorylation and greater (P<0.001) accumulation of @entity3362 / @entity2648 . CONCLUSIONS: Our clinical and in vitro data support the theory that this combination of @entity6398 reduced diastolic pressure by potentiating eNOS activation and @entity842 production. Such supplements may have clinical relevance as stand-alone or adjunct therapy to help reduce BP.
