Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and XXXX as major components of matrix remodeling.

@entity650 @entity2289 @entity5735 @entity28 @entity7 @entity3334 @entity43500 @entity1254 @entity2533 @entity71318 @entity5523 @entity1941 @entity687 @entity1386
Mature adipocytes can reverse their phenotype to become fibroblast-like cells. This is achieved by ceiling culture and the resulting cells, called @entity1386 ( @entity1386 ) cells, are multipotent. Beyond the potential value of these cells for regenerative medicine, the dedifferentiation process itself raises many questions about cellular plasticity and the pathways implicated in cell behavior. This work has been performed with the objective of obtaining new information on adipocyte dedifferentiation, especially pertaining to new targets that may be involved in cellular fate changes. To do so, omental and subcutaneous mature adipocytes sampled from severely @entity28 subjects have been dedifferentiated by ceiling culture. An experimental design with various time points along the dedifferentiation process has been utilized to better understand this process. Cell size, gene and protein expression as well as cytokine secretion were investigated. @entity650 , @entity687 , @entity2289 and @entity7 secretion were increased during dedifferentiation, whereas @entity43500 secretion was transiently increased. A marked decrease in expression of mature adipocyte transcripts ( @entity71318 , @entity5523 and @entity1254 ) was detected early in the process. In addition, some matrix remodeling transcripts ( @entity1941 , @entity5735 , @entity3334 and @entity2533 ) were rapidly and strongly up-regulated. @entity1941 and @entity5735 proteins were simultaneously induced in dedifferentiating mature adipocytes supporting a potential role for these enzymes in adipose tissue remodeling and cell plasticity.
