Migration of XXXX lymphocytes in vitro. I. Normal lymph node lymphocytes.

@entity19 @entity1507 @entity10843 @entity2389 @entity73007 @entity15
Normal unstimulated @entity19 lymph node lymphocytes (LNLs) migrated into filters in a gradient of @entity73007 ( @entity73007 ), heat-inactivated mouse serum (HI- @entity15 ), or zymosan-activated mouse serum (ZAS). Blind well chemotaxis chambers with 5-micrometer pore size cellulose @entity1507 membranes were used. Migration was assessed both by the leading front technique and the mean aggregate number. A concentration of 2.5 x 10(6) LNLs/ml or greater was needed to detect migration. Migration of LNLs to 1% @entity73007 was time dependent and was inhibited by @entity2389 . Comparison of the migration patterns of LNLs, neutrophils, and macrophages revealed that all cell types were responsive to @entity73007 . LNLs responded as well to HI- @entity15 as they did to @entity73007 , neutrophils responded less well to HI- @entity15 than to @entity10843 , and macrophages did not respond to HI- @entity15 . The LNL response to ZAS was significantly greater than the response to @entity73007 to HI- @entity15 and neutrophils and macrophages also responded strongly to ZAS. The migration of LNLs from various @entity19 strains to @entity73007 revealed that the LNLs from different @entity19 strains possess varying degree of motility. The factor in mouse serum which induced migration was not strain specific. The LNLs from peripheral (inguinal, axillary, and brachial) nodes demonstrated greater motility in response to @entity73007 than mesenteric LNLs. Using the checkerboard assay to discriminate chemotaxis from chemokinesis, mouse serum appeared to be solely chemokinetic when the leading front technique was used. However, using the mean aggregate number technique, mouse serum was determined to be both chemokinetic acid chemotactic for LNLs. The results indicate that the method can be reliably used to study those factors which influence the motility of normal or altered populations of lymphocytes.
