L-selectin and beta2-integrin expression on circulating XXXX polymorphonuclear leukocytes during endotoxin @entity1271 .

@entity558 @entity701 @entity1271 @entity7637 @entity291 @entity526
The aim of this in vivo study was to examine the effect of intramammarily administered endotoxin (lipopolysaccharide, @entity558 ) on the expression of L-selectin (CD62L) and the beta2-integrin subunits @entity7637 and CD18 on circulating @entity291 PMN. Six early lactating @entity291 were infused with @entity526 @entity558 . The adhesion molecules under study were stained at the cell surface and analyzed flow cytometrically. In addition, some of the clinical parameters associated with adhesion molecule mobilization such as fever, blood @entity701 levels, somatic cell count (SCC), and total and differential blood leukocyte count were measured. In analogy with observations during clinical coliform @entity1271 , a progressive decrease of CD62L expression levels was observed early after @entity558 infusion, concomitantly with a continuous rise of @entity7637 and CD18 density. However, no correlation was found between the kinetics of @entity7637 and CD18 density. The initial changes in adhesion molecule expression paralleled the decrease in blood PMN numbers, together with the increase in rectal temperature, @entity701 levels, SCC, and number of circulating immature PMN. In conclusion, intramammarily administered @entity558 seems to play an important role in modulating adhesion receptor expression on circulating @entity291 PMN. Interestingly, in contrast to coliform @entity1271 , the net CD18 variation is not principally influenced by @entity7637 upregulation during endotoxin administration. The knowledge of adhesion molecule kinetics in relation to the different parameters evaluated in the present study contributes to an improved understanding of the inflammatory reaction.
