Flow cytometric analysis of DNA content in XXXX @entity150 and irrigation fluids.

@entity1 @entity2007 @entity5 @entity189 @entity150
Flow cytometry (FCM) was used to study the DNA distribution of 99 @entity5 biopsy specimens and 41 bladder irrigation samples from @entity1 with transitional cell @entity150 . For @entity5 biopsy and cystectomy specimens, the frequency of aneuploidy increased with advancing @entity5 stage and grade. All T0 @entity5 were diploid. Twenty-seven percent of T1, 71.4% of T2, and 75% of T3 and T4 @entity5 were @entity2007 . All Grade I @entity5 were diploid. Thirty percent of Grade II and 76.9% of @entity5 were @entity2007 . The frequency of @entity5 in the early stages (Ta, T1) is similar to the incidence of subsequent progression by these @entity5 described in the literature. For irrigation fluids, the relationship between grade and stage and the frequency of aneuploidy was similar to the relationship seen with @entity5 specimens. All four @entity1 with @entity189 in situ had @entity2007 cells in their irrigations. The comparison of FCM data of bladder biopsy and bladder irrigation from the same cystoscopic evaluation suggests adequate representation of @entity5 cells in the irrigation fluids for almost all cases. The authors conclude that DNA ploidy analysis by FCM appears useful in a clinically important group of @entity1 with @entity2007 superficial @entity5 of moderate or high grade. Bladder irrigation analysis appears useful in the follow-up of @entity1 with a history of @entity189 in situ and those with @entity5 .
