Hydrogen Sulfide Treatment Mitigates XXXX during Cold Storage and Improves Early Transplant Kidney Function and Survival Following Allogeneic Renal Transplantation.

@entity3958 @entity8 @entity63 @entity39 @entity26493 @entity8835 @entity35
PURPOSE: @entity63 -reperfusion injury is unavoidable during organ transplantation. Prolonged @entity63 -reperfusion injury is detrimental to short-term and long-term graft function and survival. @entity3958 is a recently characterized, endogenously produced gaseous molecule with important physiological roles that has been shown to be cytoprotective during tissue @entity63 -reperfusion injury. The current study aimed to determine whether @entity3958 could mitigate cold @entity8 in the clinically relevant context of allogeneic renal transplantation. MATERIALS AND METHODS: Following bilateral native nephrectomy Lewis @entity35 underwent renal transplantation with kidneys from Brown Norway donor @entity35 that were flushed with cold (4C) standard University of Wisconsin preservation solution (University of Wisconsin preservation solution group) or cold University of Wisconsin preservation solution plus 150  M @entity8835 ( @entity3958 group) solution. Kidneys were stored for 6 hours at 4C in the same solution. Recipient animals were monitored for 14 days or until sacrifice using metabolic cages to assess various parameters of renal graft function. RESULTS: @entity3958 treatment improved early allograft survival and function, and decreased early levels of @entity39 , apoptosis and @entity26493 compared to University of Wisconsin preservation solution alone. @entity3958 treatment did not affect allograft rejection. Rather, it modulated the early allograft transcriptome to decrease the expression of @entity8 , coagulation and cellular stress response genes, and increase the expression of cellular proliferation and Ifn-y induced genes compared to University of Wisconsin preservation solution alone. CONCLUSIONS: To our knowledge our findings are the first to show that @entity3958 protects donor kidneys against @entity63 -reperfusion injury in the context of allogeneic renal transplantation. This potentially represents a novel cost-effective therapeutic solution to mitigate @entity63 -reperfusion injury and improve the clinical outcomes of renal transplantation.
