XXXX disease ( @entity62 ) in Australia.

@entity15103 @entity39197 @entity75 @entity62 @entity72755
The index case of @entity62 ( @entity62 ) is presented in adult broodstock prawns @entity15103 from the Flinders River in western Queensland, Australia, in mid-2007. Histological examination revealed extensive myonecrosis with massive infiltration of myonuclei and some haemocytes. Juveniles from the same broodstock but not from 3 other families displayed @entity39197 . Low-grade chronic mortalities approaching 100% over 1 yr occurred. Reverse transcriptase polymerase chain reactions (RT-PCR) were attempted for both @entity72755 (MrNV) with 2 sets of primers and for the satellite virus, extrasmall virus (XSV). All 3 PCRs generated amplicons of the expected sizes. Basic local alignment search tool (BLAST) analyses of the 3 consensus sequences identified a 91% match with MrNV viral capsid protein gene, 96% match with MrNV RNA-directed RNA polymerase gene, and a 99% match with @entity15103 XSV capsid protein gene. The clinical signs, @entity75 and RT-PCR amplicons could be reproduced in @entity15103 inoculated with cell-free extracts fulfilling River's postulates. We conclude that this is an endemic strain of MrNV as the sequences are dissimilar to strains of MrNV circulating around Asia and the Americas. This case only poorly meets the Office International des Epizooties (OIE) case definition for @entity62 due to the age of the prawns involved and the nature of the inclusion bodies. Perhaps the OIE case definition needs broadening.
