De Novo Assembly of the Whole Transcriptome of the Wild Embryo, Preleptocephalus, Leptocephalus, and Glass Eel of XXXX and Deciphering the Digestive and Absorptive Capacities during Early Development.

@entity7787 @entity948 @entity1735 @entity16932 @entity3689 @entity413 @entity165
UNASSIGNED: Natural stocks of @entity16932 ( @entity16932 ) have decreased drastically because of overfishing, habitat destruction, and changes in the ocean environment over the past few decades. However, to date, artificial mass production of glass eels is far from reality because of the lack of appropriate feed for the eel larvae. In this study, wild glass eel, leptocephali, preleptocephali, and embryos were collected to conduct RNA-seq. Approximately 279 million reads were generated and assembled into 224,043 transcripts. The transcript levels of genes coding for digestive enzymes and nutrient transporters were investigated to estimate the capacities for nutrient digestion and absorption during early development. The results showed that the transcript levels of protein digestion enzymes were higher than those of @entity1735 and lipid digestion enzymes in the preleptocephali and leptocephali, and the transcript levels of @entity3689 transporters were also higher than those of @entity413 and @entity948 transporters and the @entity165 transporter. In addition, the transcript levels of @entity413 and @entity948 transporters were significantly raising in the leptocephali. Moreover, the transcript levels of protein, @entity1735 , and lipid digestion enzymes were balanced in the glass eel, but the transcript levels of @entity3689 transporters were higher than those of @entity413 and @entity165 transporters. These findings implied that preleptocephali and leptocephali prefer high-protein food, and the nutritional requirements of @entity7787 and lipids for the eel larvae vary with growth. An online database (http://molas.iis.sinica.edu.tw/jpeel/) that will provide the sequences and the annotated results of assembled transcripts was established for the eel research community.
