Recombinant XXXX @entity1261 treatment improves macrophage suppression of granulocyte and macrophage growth after burn and burn wound infection.

@entity1 @entity295 @entity796 @entity1261 @entity19 @entity1583 @entity236 @entity413 @entity130
Granulocyte and macrophage production after burn @entity130 is significantly reduced and further compromised by endotoxin (ET). Moreover, the macrophage seems to be the major source of this @entity236 . We sought to determine if recombinant @entity1 @entity1261 (rhG-CSF), a @entity1583 growth factor that is capable of improving survival after experimental burn wound @entity295 , altered postburn macrophage-mediated marrow suppression. Groups of male BDF1 @entity19 (n = 6 to 10) receiving a 15% total body surface area burn +/- infection (B or B + I) with @entity796 were injected with 100 ng rhG-CSF twice daily. On day 3, peritoneal-elicited macrophages (5 x 10(6) cells/mL) from either rhG-CSF-treated or control (5% @entity413 in water) @entity19 were incubated +/- ET (300 ng/mL). The resultant macrophage supernatant was added to cultures of target marrow granulocyte-macrophage progenitor cells (GM-CFC) at a volume of 1:10. The GM-CFC growth as a percentage of cultures not containing macrophage supernatant were compared and reductions in the number of GM-CFC taken as an index of marrow suppression. Macrophages obtained from B and B + I animals reduced target GM-CFC growth, compared with macrophages from normal animals (B vs. normal animals p < 0.05). In addition, ET-stimulated macrophages induced further @entity236 for all three groups (p < 0.01). Macrophages from @entity1261 -treated animals caused significantly less @entity236 , compared with untreated animals for all groups (p < 0.05 to 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
