The effects of electromagnetic pulse on the protein levels of tight junction associated-proteins in the cerebral cortex, hippocampus, heart, lung, and testis of XXXX .

@entity35 @entity10261 @entity659
OBJECTIVE: To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex, hippocampus, heart, lung, and testes of @entity35 after exposure to electromagnetic pulse (EMP). METHODS: Eighteen adult male @entity35 were divided into sham and exposure groups. The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz. The expression of TJ proteins ( @entity10261 , @entity659 , actin) in the several organs was examined by western blotting. RESULTS: @entity10261 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P<0.05). No significant difference was observed for @entity659 and actin. @entity10261 levels in the hippocampus increased 1 h and 3 h post-exposure (P<0.05), and @entity659 decreased after 3 h (P<0.05); however, actin was unaffected. @entity10261 levels in the heart increased 3 h post-exposure (P<0.05), @entity659 decreased 3 h post-exposure (P<0.05), and actin increased 1 h and 3 h post-exposure (P<0.05). @entity10261 , @entity659 and actin levels in the lung decreased compared with those in the sham group (P<0.05). @entity10261 and @entity659 levels in the testes decreased 1 h and 3 h post-exposure (P<0.05), but actin showed no significant change. CONCLUSION: Exposure to EMP altered the expression levels of TJ proteins, particularly @entity10261 , in the organs of adult male @entity35 , which may induce changes in barrier structure and function.
