165 effect of injection of seminal plasma on ovulation rate, corpus luteum development, and sensitivity to @entity3009 in XXXX ( @entity7858 ).

@entity7458 @entity7858 @entity598 @entity3009
The seminal plasma (SP) of camelids contains a protein identified as b Nerve Growth Factor with capacity of induced ovulation and develop a corpus luteum. A study was designed to evaluate the effect of application of SP on the interval of time from injection of stimulus to the ovulation and corpus luteum (CL) size (Experiment 1, n=24) and on the sensitivity of CL to the injection of @entity3009 (196  g of tiaprost) (PG) at different periods from ovulation (Experiment 2, n=86). Exp. 1: Adult female @entity7858 with presence of a follicle >= 7mm were assigned to the application of 1mL of SP via IM (T: n=12) or application of 50  g of @entity598 of busereline IM (T2: n=12). Exp. 2: @entity7858 with presence of a follicle >= 7mm were induced to ovulation with 50  g of @entity598 of busereline or 1mL IM of SP. Animals were evaluated by ultrasound to confirm the ovulation and were assigned to the following treatment: T1 (n=8): SP+PG Day 4; T2 (n=8): @entity7458 +PG Day 4; T3 (n=8): SP+PG Day 5; T4 (n=8): @entity7458 +PG Day 5; T5 (n=8): SP+PG Day 6; T6 (n=8): @entity7458 +PG Day 6; T7 (n=8): SP+PG Day 7; T8 (n=8): @entity7458 +PG Day 7; T9 (n=8): SP+PG Day 8; T10 (n=8): @entity7458 +PG Day 8 and T11 (n=6) (Control): Application of 1mL of saline solution. The animals were evaluated by ultrasound with an Aloka SSD500 (Aloka, Tokyo, Japan) and 7.5-MHz linear transducer each 2h (Exp. 1) and each 12h (Exp. 2) after of application of PG. In Exp. 1, the ovulation rate was 95.7% to T1 and T2 and an interval of time between injection of stimulus and ovulation was 27.4  2.5h and 26.8  1.8 to T1 and T2, respectively. In Exp. 2, luteolysis was 0.0%, 0.0%, 25.0%, 0.0%, 100.0%, 100.0%, 100.0%, 100.0%, 100.0%, 100.0%, and 0.0% for the treatments T1, T2, T3, T4, T5, T6, T7, T8, T9, T10, and T11 respectively. The results suggest that no differences exist between in the ovulation rate and interval to the ovulation between the application of @entity7458 or SP and that the CL was sensible at Day 5 to the @entity3009 respect SP and with similar response to the sensibility of CL from Day 6 to Day 8.
