Differential @entity35 T cell recognition of @entity12827 -released fragments of mycobacterial 65 kDa XXXX after immunization with either the recombinant protein or whole mycobacteria.

@entity12827 @entity625 @entity28379 @entity1753 @entity19514 @entity3795 @entity35
T cells specific for the mycobacterial 65 kDa @entity19514 ( @entity28379 ) play a pivotal role in the development of @entity1753 ( @entity1753 ) in Lewis @entity35 . Upon adoptive transfer, CD4+ T cells recognizing a particular @entity28379 epitope trigger the onset of disease. Activation of @entity28379 -reactive T cells can be achieved by immunization with heat-killed mycobacteria in mineral oil--complete Freund's adjuvant ( @entity3795 )--or with purified recombinant @entity28379 . @entity625 , however, will only develop after immunization with @entity3795 . In fact, preimmunization with @entity28379 protects against any subsequent attempt to induce @entity1753 . In this study, we examined polyclonal lymph node cell responses in Lewis @entity35 , immunized with either @entity3795 or purified recombinant @entity28379 in incomplete Freund's adjuvant, to a set of @entity28379 fragments generated by a mild digestion with @entity12827 . Proliferative responses to several @entity28379 fragments varied with the type of antigen used for immunization. A @entity12827 -released fragment, identified as residues 376-408, preferentially triggered proliferation of @entity35 T cells after @entity28379 immunization. Preimmunization of Lewis @entity35 with this peptide delayed the onset and reduced the severity of @entity1753 . Preimmunization with another fragment which was preferentially recognized after @entity3795 immunization, representing residues 40-60, did not have such a protective effect. Our findings suggest the presence of mycobacterial @entity28379 determinants that selectively trigger @entity1753 -regulating T cells and illustrate that @entity12827 may be used as an experimental tool to generate such determinants.
