Comparison of growth factor adsorbed scaffold and conventional scaffold with growth factor supplemented media for primary XXXX articular chondrocyte 3D culture.

@entity1 @entity13275 @entity8273 @entity1936 @entity794 @entity1934 @entity1935
BACKGROUND: Cartilage tissue engineering offers new strategies in repairing damaged cartilage. Scaffolds have been used for the in vitro and in vivo procedures for this application, which demonstrates the compatible biological and physical properties that mimic natural tissues. Several types of scaffolds were used and had different effects on cell functions. The study was designed to develop a functional gelatin scaffold by adsorption of @entity8273 ( @entity8273 ) and the @entity1934 ( @entity1934 ) in a commercially available gelatin scaffold. RESULTS: The biological properties of @entity1 articular chondrocytes were investigated during a 21-day cultivation embedded in either @entity8273   +   @entity1934 adsorbed scaffolds or the conventional supplemented method. The rising of proliferation of chondrocytes embedded in adsorbed scaffolds was observed at day 17 and 21 of cultivation (1.27 and 1.28 fold, respectively). The chondrogenic gene expression of the chondrocytes embedded in @entity8273   +   @entity1934 adsorbed scaffolds significantly increased: @entity1935 (1.65 fold), @entity1936 (7.65 fold) and @entity794 (1.83 fold). Remarkably, over the 21  days of cultivation, @entity8273   +   @entity1934 adsorbed scaffolds promoted the extracellular matrix molecules production with higher accumulation of @entity8273 (1.2 fold), collagen (1.42 fold) and @entity13275 (1.41 fold). Moreover, the cell population and extracellular matrix production, which were examined by a histological analysis and a scanning electron microscope, were correlated with the biochemical analysis. CONCLUSION: A small amount of @entity8273 and @entity1934 initially adsorbed in the scaffolds (70   g and 10  ng, respectively) was consumed over the 21-day cultivation. The @entity8273   +   @entity1934 adsorbed gelatin scaffold is effective and more suitable than the conventional supplemented method for the in vitro assessment of @entity1 chondrocyte 3D culture.
