Angiogenesis as a prognostic marker in XXXX with conventional adjuvant chemotherapy: a multiparametric and immunohistochemical analysis.

@entity1 @entity0 @entity588 @entity146 @entity5 @entity2931 @entity1420 @entity2279 @entity876 @entity1722
It has now been clearly established that quantitative immunohistochemical methods applied to @entity5 angiogenesis under suitable quality control conditions are a powerful prognostic tool for use in the initial assessment of @entity0 . Appropriate parameters for predicting the @entity146 of @entity5 and their sensitivity to treatment are, however, still required. To determine whether the microvessel count (MVC) may serve to predict the chemotherapeutic response, a retrospective study was carried out on a series of 162 @entity1 with @entity0 , who were all treated with the same standard adjuvant chemotherapy. Angiogenesis was assessed by performing @entity2279 immunostaining and MVC per mm2. Several other factors such as @entity876 , @entity1722 , @entity588 , and Ki67 were also measured, and their prognostic value was compared with that of the MVC. The MVC was not found to be correlated with any of the other prognostic parameters, but turned out to be of great prognostic value whatever the threshold value chosen, which suggests that it is continuously valid at all levels. The median value of the MVC (43.5 per mm2) divided this series into two significantly different prognostic categories, in terms of both disease-free survival (P = 0.0002) and overall survival (P = 0.037). Univariate analysis showed that most of the parameters analysed were of prognostic value regarding the disease-free survival, namely grade (P = 0.029), mitotic index (P = 0.049), size (P = 0.015), @entity2931 receptors (P = 0.022), @entity1420 receptors (P = 0.018), @entity876 (P = 0.0045), @entity1722 (P = 0.046), and Ki67 (P = 0.0008). As regards overall survival, grade and @entity1722 showed a loss of prognostic value. In multivariate analysis on disease-free survival, the MVC was the most accurate prognostic factor (RR = 2.64), followed by Ki67 (RR = 2.06) and @entity876 (RR = 1.69). With respect to overall survival, the MVC ranked third among the prognostic parameters analysed. Standard chemotherapy did not reduce the high prognostic value of the MVC performed on @entity5 angiogenesis. This suggests that the MVC may predict the degree of resistance to chemotherapy. @entity1 with high levels of angiogenesis, particularly node-negative @entity1 , might therefore be able to benefit from adjuvant therapy of another kind.
