OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of @entity1476 / XXXX genes: a novel mechanism for cardiovascular diseases.

@entity1 @entity1476 @entity1495 @entity15702 @entity731 @entity15894
MicroRNAs (miRNAs), small noncoding RNAs, can control gene expression by binding to their target genes for degradation and/or translational repression. Epigenetic mechanisms are defined as heritable changes in gene expression that do not involve coding sequence modifications. Both mechanisms play an important role in maintaining physiological functions and are also related to disease development. However, few studies report that miRNA-mediated epigenetic regulations are involved in @entity731 . In the present study, oxidized low-density lipoprotein (oxLDL) significantly increased primary @entity1 aortic smooth muscle cell (HASMC) migration through @entity1476 / @entity1495 up-regulation associated with decreased DNA methylation levels. Either mRNA or protein level of @entity15894 ( @entity15894 ) showed a dose-dependent down-regulation in oxLDL-mediated HASMCs. Knockdown @entity15894 expression enhanced oxLDL-induced DNA demethylation levels of @entity1476 / @entity1495 . The expression of miRNA-29b ( @entity15702 ), directly targeting @entity15894 , was up-regulated by oxLDL treatment in a dose-dependent manner. OxLDL-mediated @entity1476 / @entity1495 up-regulation, @entity15894 down-regulation, and DNA demethylation were all attenuated after knockdown @entity15702 expression by antagomiR-29b. We find that oxLDL can up-regulate @entity15702 expression, resulting in @entity15894 down-regulation in HASMCs and epigenetically regulated @entity1476 / @entity1495 genes involved in cell migration. These results show that miRNA-mediated epigenetic regulation may be a novel mechanism in @entity731 .
