Intracellular signalling molecules as immunohistochemical markers of normal and neoplastic XXXX leucocytes in routine biopsy samples.

@entity1 @entity18226 @entity27420 @entity2060 @entity706 @entity784 @entity2109 @entity10255 @entity7985 @entity13788 @entity1892 @entity7984 @entity30930
We have investigated whether intracellular signal transduction molecules can be used as immunohistological markers of normal and neoplastic @entity1 leucocytes in routine tissue sections. We obtained selective labelling of white cells for eight such molecules (the 'linker' molecules @entity30930 and @entity13788 , the Src family kinases @entity18226 , @entity7984 , @entity10255 and @entity2060 , and the phospholipases @entity7985 and @entity27420 ). Antibodies to @entity30930 and @entity7985 selectively labelled T cells, and antibodies to @entity13788 , @entity18226 , @entity7984 , @entity10255 and @entity27420 labelled B cells (although @entity7984 immunostaining was restricted to mantle zone B cells). Antibodies to the @entity10255 and @entity2060 kinases labelled probable thymocyte precursors at the periphery of the thymic cortex. In addition to lymphoid cells, several other leucocyte types were immunostained (e.g. @entity30930 , @entity18226 , @entity10255 and @entity2060 were found in megakaryocytes, myeloid cells and/or macrophages, and @entity27420 was detected in arterial endothelium). @entity30930 and @entity7985 were found in most @entity784 studied, and some @entity1892 were also positive for @entity7985 (e.g. diffuse large cell and @entity2109 ). The five B cell-associated markers were found in most B-cell non-Hodgkin's @entity706 , although some diffuse large @entity1892 were negative (e.g. for @entity18226 ) and anti- @entity7984 tended not to stain small B-cell neoplasms. The observation that a range of leucocyte signalling molecules can be detected in routine biopsies offers new possibilities for studying normal and neoplastic @entity1 white cells in diagnostic tissue samples.
