Role of ROS-mediated autophagy in radiation-induced bystander effect of XXXX cells.

@entity1 @entity4523 @entity26 @entity3733 @entity8783 @entity157 @entity21684
UNASSIGNED: Purpose: Autophagy plays a crucial role in cellular response to ionizing radiation, but it is unclear whether autophagy can modulate radiation-induced bystander effect (RIBE). Here, we investigated the relationship between bystander damage and autophagy in @entity1 @entity157 cells of HepG2. Materials and methods: HepG2 cells were treated with conditioned medium (CM) collected from 3 Gy y-rays irradiated @entity157 HepG2 cells for 4, 12, or 24 h, followed by the measurement of @entity4523 ( @entity4523 ), intracellular reactive @entity26 species (ROS), mitochondrial membrane potential (MMP), and protein expressions of @entity21684 and @entity8783 in the bystander HepG2 cells. In some experiments, the bystander HepG2 cells were respectively transfected with @entity21684 small interfering RNA (siRNA), @entity8783 siRNA or treated with 1% @entity3733 ( @entity3733 ). Results: Additional @entity4523 and mitochondrial dysfunction coupled with ROS were induced in the bystander cells. The expressions of protein markers of autophagy, @entity21684 -II/ @entity21684 -I and @entity8783 , increased in the bystander cells. The inductions of bystander @entity4523 and overexpressions of @entity21684 and @entity8783 were significantly diminished by @entity3733 . However, when the bystander cells were transfected with @entity21684 siRNA or @entity8783 siRNA, the yield of bystander @entity4523 was significantly enhanced. Conclusion: The elevated ROS have bi-functions in balancing the bystander effects. One is to cause @entity4523 and the other is to induce protective autophagy.
