The amount of citrullinated proteins in synovial tissue is related to serum anti-cyclic citrullinated peptide (anti- XXXX ) antibody levels.

@entity1 @entity30445 @entity571 @entity72051 @entity22403 @entity5810 @entity309
UNASSIGNED: The objective of this study was to determine the relationship between citrullinated proteins in synovial tissue with peripheral anti-citrullinated peptides autoantibodies (ACPA) and peptidylarginine deiminase (PADI) @entity30445 , @entity72051 , and @entity22403 messenger RNA (mRNA) expressions in synovial tissue and fibroblast-like synoviocytes in @entity309 ( @entity309 ) @entity1 . Eleven @entity309 and 12 @entity571 ( @entity571 ) @entity1 who underwent knee replacement surgery were studied. We detected citrullinated proteins in synovial tissue homogenates by western blot and serum ACPA by ELISA to anti-cyclic citrullinated peptide (anti- @entity5810 ) antibodies, and @entity30445 , @entity72051 , and @entity22403 mRNA expressions in synovial tissue and in fibroblast-like synoviocytes. @entity1 with high amount of citrullinated proteins in synovial tissue (3 out of 7) have high levels of anti- @entity5810 in serum. However, in the remaining 4 @entity1 , the amount of synovial citrullinated proteins was minimal and their sera showed low levels of anti- @entity5810 antibodies. Furthermore, we observed an increase in @entity30445 mRNA expression in @entity309 synovial tissue compared with @entity571 @entity1 (p   =   0.02). We detected @entity72051 mRNA in the synovial tissue of @entity309 @entity1 , but not in the tissue of @entity571 @entity1 . Even though fibroblast-type synoviocytes in @entity309 are not the main source of PADs in the synovial tissue, they express @entity30445 mRNA moderately, @entity22403 mRNA weakly, while there is no detectable expression of @entity72051 mRNA. In conclusion, we found a variety of citrullinated proteins in the synovial tissue of @entity309 @entity1 and the amount of such proteins is related to serum concentration of anti- @entity5810 antibodies. We identified the presence of  @entity72051 mRNA expression in synovial tissue and @entity30445 and @entity22403 mRNA expressions in fibroblast-like synoviocytes from @entity1 with @entity309 .
