A limited role for regulatory T cells in post- XXXX neovascularization.

@entity1619 @entity63 @entity19 @entity2047 @entity3783 @entity1961 @entity3744
Recently, it was demonstrated that arteriogenesis is enhanced in @entity19 deficient in regulatory T cells ( @entity1961 (+) @entity3783 (+) @entity3744 (+) T cell), which can suppress effector T cell responses. The present study investigates the effects of these regulatory T cells on arteriogenesis in more detail by either specific expanding or depleting regulatory T cells. Hind limb @entity63 was induced by electro-coagulation of the femoral artery in @entity19 . @entity1619 cells were either expanded by injecting @entity19 with a complex of @entity2047 with the @entity2047 monoclonal antibody JES6-1, or depleted by anti- @entity3783 antibody or diphtheria toxin injections in DEREG @entity19 (depletion of regulatory T cells). Blood flow restoration was monitored using laser Doppler perfusion imaging. Collateral arteries were visualized by immunohistochemistry. @entity1619 cell expansion led to a moderate though significant suppression of blood flow restoration after @entity63 induction. Surprisingly, depletion of regulatory T cells resulted in minor increase on blood flow recovery. However, collateral and capillary densities in the post- @entity63 skeletal muscle were significantly increased in DEREG @entity19 depleted for regulatory T cells. The presence of regulatory T cells after @entity63 induction when analysed in non-depleted DEREG @entity19 could be demonstrated by green fluorescent protein staining only in lymph nodes in the @entity63 area, and not in the @entity63 muscle tissue. The current study demonstrates that, even under conditions of major changes in regulatory T cell content, the contribution of regulatory T cells to the regulation of the arteriogenic response is only moderate.
