Maturation-dependent expression of @entity6300 -binding proteins on the cell surface of XXXX monocyte-derived dendritic cells.

@entity1 @entity558 @entity6300 @entity619 @entity21345 @entity6301 @entity4015 @entity688 @entity3027
The expression and cell surface levels of many important receptors are dependent on the maturation stage of dendritic cells (DCs), and related to the unique function of immature and mature DCs. In this report, we show for the first time that @entity1 monocyte-derived DCs express two types of @entity6300 -receptors, @entity21345 and @entity4015 . Furthermore, immature DCs secrete detectable amount of @entity6300 into the culture supernatant. Immature DCs express higher cell surface levels of both C1qRs than mature ones, while the total @entity6301 protein and mRNA levels remain the same. The following experimental evidence supports this conclusion. (1) Inflammatory cytokines and @entity558 , which induce maturation of DCs, downregulate surface expression of both @entity6301 molecules. (2) Cytokines and drugs ( @entity688 , @entity3027 , @entity619 ) that keep DCs phenotypically and functionally immature significantly upregulate the cell surface expression of both C1qRs. (3) Neither of these treatments changed the intracellular @entity21345 level nor the @entity21345 mRNA levels measured by real-time RT-PCR. The elevated surface expression of C1qRs on DCs has been found not to be due to increased apoptosis or cell death as the result of DC treatment. Taken together, these data show that @entity1 monocyte-derived DCs express @entity21345 and @entity4015 , their expression on the cell surface is maturation dependent and imature DCs secrete @entity6300 . These data strongly suggest the role of C1qRs in immature DC function and in the regulation of immune processes.
