@entity1 kallikrein 14: a new potential biomarker for XXXX .

@entity1 @entity43 @entity16914 @entity5344 @entity5 @entity10056 @entity19 @entity563 @entity6881 @entity63416 @entity1486
@entity1 @entity63416 ( @entity63416 ) is a recently discovered member of the tissue kallikrein family of secreted @entity563 proteases, which includes @entity16914 / @entity1486 , the @entity5 biomarker to date. Given that @entity63416 is hormonally regulated, differentially expressed in endocrine-related @entity5 , and a prognostic marker for @entity5344 at the mRNA level, we hypothesize that its encoded protein, @entity6881 , like @entity16914 / @entity1486 , may constitute a new biomarker for endocrine-related @entity5 . The objective of this study was to generate immunological reagents for @entity6881 , to develop an ELISA and immunohistochemical techniques to study its expression in normal and cancerous tissues and biological fluids. Recombinant @entity6881 was produced in @entity10056 , purified by affinity chromatography, and injected into @entity19 and rabbits for polyclonal antibody generation. Using the @entity19 and rabbit antisera, a sandwich-type immunofluorometric ELISA and immunohistochemical methodologies were developed for @entity6881 . The ELISA was sensitive (detection limit of 0.1 micro g/liter), specific for @entity6881 , linear from 0 to 20 micro g/liter with between-run and within-run coefficients of variation of <10%. @entity6881 was quantified in @entity1 tissue extracts and biological fluids. Highest levels were observed in the breast, skin, prostate, seminal plasma, and amniotic fluid, with almost undetectable levels in normal serum. @entity6881 concentration was higher in 40% of @entity43 tissues compared with normal ovarian tissues. Serum @entity6881 levels were elevated in a proportion of @entity1 with ovarian (65%) and breast (40%) @entity5 . Immunohistochemical analyses indicated strong cytoplasmic staining of @entity6881 by the epithelial cells of normal and malignant skin, ovary, breast, and testis. In conclusion, we report the first ELISA and immunohistochemical assays for @entity6881 and describe its distribution in tissues and biological fluids. Our preliminary data indicate that @entity6881 is a potential biomarker for @entity5344 .
