XXXX -induced drought stress on calli of Trigonellafoenum-graecum L. Var. RMt-303.

@entity2656 @entity274 @entity30972 @entity40404 @entity12682 @entity1234 @entity3015 @entity1521 @entity38 @entity9050
Different explants of fenugreek, T. foenum-graecum L. (Var. RMt-303), were compared for their callus induction and subsequent shoot regeneration capabilities on Murashige and Skoog media supplemented with different phytohormones in varying concentration. The highest percentage of callus induction frequency was observed in 1 ppm @entity40404 ( @entity3015 ). Maximum shoots were induced on media supplemented with 0.5 ppm BAP using leaf and stem tissues as explants. However, root tissues showed only callusing with no subsequent shooting. Cotyledonary node responded better than hypocotyls in terms of shoot induction on media supplemented with @entity30972 (0.1 ppm). The callus was subjected to drought stress as simulated by reduced water potential of growth media due to addition of @entity2656 . Calli could withstand -2 MPa water potential till 30 days indicating that the drought stress tolerance mechanisms are functional in this variety. Chlorophyll a and b and total @entity38 , @entity1521 and total phenolic contents, total peroxidase and catalase activities increased under stress conditions suggesting the tolerance of callus to drought stress. However, @entity9050 peroxidase, @entity12682 peroxidase activities were found to decrease slightly. @entity1234 and @entity274 contents were found to decrease while only a slight disturbance was found in membrane stability index. These results underline the mechanisms that are crucial for drought stress tolerance in fenugreek.
