Distribution of @entity140 -selective glutamate receptor subunits in the XXXX hippocampus and cerebellum.

@entity1 @entity2374 @entity1399 @entity11587 @entity7336 @entity71621 @entity140 @entity7335
The distribution of @entity140 -selective subunits, @entity7335 -4, was determined in the @entity1 hippocampus and cerebellum by in situ hybridization and immunocytochemistry. In the hippocampus, in situ hybridization revealed that @entity7335 and @entity7336 mRNAs were similarly distributed and highly expressed in the dentate gyrus, with lower levels in the CA regions. @entity2374 and @entity11587 mRNAs were expressed at very low levels. Immunocytochemical studies showed that @entity7335 - and @entity7336 /3-immunoreactivity were highest in the dentate molecular and granular layers. In the CA regions, @entity7335 and @entity7336 /3 staining was observed in pyramidal cell bodies and surrounding neuropil and was more intense in @entity71621 compared with @entity1399 . @entity11587 -immunoreactivity was low throughout the hippocampus. In the cerebellum, @entity7335 and @entity11587 transcripts were expressed in the granular and Purkinje cell/Bergmann glia layers. @entity7336 mRNA was highly expressed in the granular layer and individual Purkinje cells, while @entity2374 mRNA was not detectable in the cerebellum. @entity7335 - and @entity11587 -immunoreactivity were localized to Purkinje cells and putative Bergmann glia, as well as their processes extending into the molecular layer. @entity7336 /3 staining was intense in Purkinje cells, with moderate staining in the granular layer. Thus, @entity7335 -4 subunits are differentially distributed in the hippocampus and cerebellum. In addition, the distribution of subunit mRNA and protein correlate well with each other and with the glutamatergic neuroanatomy of the hippocampus and cerebellum.
