Rapid quantitative analysis of human serum XXXX species using MALDI-TOF mass spectrometry with lipid hydrolase treatment.

@entity844 @entity66 @entity4049 @entity1042 @entity10779 @entity3636 @entity8030 @entity3900 @entity505 @entity4291
BACKGROUND: @entity4291 (SM) is a key component of extracellular membranes and lipoproteins, and plays roles in cell signaling and as a component of lipoproteins. SM species differ in terms of @entity844 (FA) composition. However, no simple, rapid, quantitative assay for identifying different SM species has yet been reported. In this study, lipid hydrolase treatment and @entity66 ( @entity66 ) were used to identify serum SM species. METHODS: Sera were collected from healthy young individuals. To identify SM species, sera were treated with @entity3900 and lipoprotein lipase, and lipids were extracted using the standard @entity3636 / @entity1042 (2/1 v/v) method. RESULTS: We detected 15 peaks from serum using @entity66 , which were assigned to SM species bound with FA components ranging from @entity10779 :0 to C24:2. The most prominent serum SM species was SM [C16:0], which accounted for approximately 26% of serum SM. Some SM species contained an odd- @entity505 FA ( @entity10779 , @entity8030 , and @entity4049 ), and these accounted for approximately 4% of serum SM. The reproducibility of major SM species within and between application positions on MS-sample plate was CV=3.0%-7.9% and CV=3.1%-6.8%, respectively. The concentration and dilution ratio were linearly related. The SM species composition of 10 healthy young subjects showed a similar profile. CONCLUSIONS: We developed a rapid, and quantitative method for identifying serum SM species using lipid hydrolase treatment and @entity66 . This method will be suitable for clinical laboratory studies to examine the associations between SM species and disease states.
