Engineering mediator-based electroactivity in the obligate aerobic bacterium XXXX .

@entity796 @entity24206 @entity11097 @entity26 @entity15250 @entity26731 @entity72046
@entity15250 strains are being developed as microbial production hosts for production of a range of amphiphilic and hydrophobic biochemicals. @entity15250 's obligate aerobic growth thereby can be an economical and technical challenge because it requires constant rigorous aeration and often causes reactor foaming. Here, we engineered a strain of @entity26731 that can produce @entity72046 redox-mediators from @entity796 to allow partial redox balancing with an electrode under @entity26 -limited conditions. @entity796 is known to employ its @entity72046 -type redox mediators for electron exchange with an anode in bioelectrochemical systems (BES). We transferred the seven core @entity72046 biosynthesis genes phzA-G and the two specific genes phzM and phzS required for @entity24206 synthesis from @entity796 on two inducible plasmids into @entity26731 . The best clone, @entity15250 pPhz, produced 45 mg/L @entity24206 over 25 h of growth, which was visible as blue color formation and is comparable to the @entity24206 production of @entity796 . This new strain was then characterized under different @entity26 -limited conditions with electrochemical redox control and changes in central energy metabolism were evaluated in comparison to the unmodified @entity26731 . In the new strain, @entity72046 synthesis with supernatant concentrations up to 33  g/mL correlated linearly with the ability to discharge electrons to an anode, whereby @entity11097 served as the dominating redox mediator. @entity15250 pPhz sustained strongly @entity26 -limited metabolism for up to 2 weeks at up to 12  A/cm(2) anodic current density. Together, this work lays a foundation for future @entity26 -limited biocatalysis with @entity15250 strains.
