Down-regulation of microRNA-223 promotes degranulation via the XXXX / @entity584 pathway by targeting @entity2856 in mast cells.

@entity4284 @entity584 @entity2306 @entity10019 @entity10412 @entity56 @entity2856
BACKGROUND: Mast cells play a central role in @entity56 and inflammatory disorders by inducing degranulation and inflammatory mediator release. Recent reports have shown that miRNAs play an important role in inflammatory response regulation. Therefore, the role of @entity10019 in mast cells was investigated. METHODS: The expression of @entity10019 was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) in immunoglobulin E (IgE)-mediated mast cells. After successful @entity10019 inhibition by transfection, degranulation was detected in IgE-mediated mast cells. The phosphorylation of @entity4284 and @entity584 were examined using western blotting. NF-kB was tested using electrophoretic mobility shift assay. @entity10412 -inhibitor ( @entity2306 ) was used to investigate whether the @entity10412 / @entity584 pathway was essential for mast cell activation. The TargetScan database and a luciferase reporter system were used to identify whether @entity2856 ( @entity2856 ) is a direct target of @entity10019 . RESULTS: @entity10019 expression was up-regulated in IgE-mediated mast cells, whereas its down-regulation promoted mast cell degranulation. Levels of @entity4284 and @entity584 phosphorylation as well as NF-kB were increased in @entity10019 inhibitor cells. @entity2306 could block the @entity10412 / @entity584 signaling pathway and rescue the promotion caused by suppressing @entity10019 in mast cells. @entity2856 was identified as a direct target of @entity10019 . CONCLUSIONS: These findings suggest that down-regulation of @entity10019 promotes degranulation via the @entity10412 / @entity584 pathway by targeting @entity2856 in mast cells.
