Prognostic significance of DNA content and S-phase fraction in XXXX analyzed by image cytometry.

@entity43 @entity1 @entity41 @entity10359 @entity3
The objective of this study was to evaluate the prognostic significance of DNA ploidy and S-phase fraction ( @entity10359 ) in @entity43 analyzed by image cytometry. Frozen tissue of 103 @entity43 was analyzed for DNA ploidy and @entity10359 with a Cell Analysis System Image Analyser (CAS 200, Becton-Dickinson) and correlations with clinical and histomorphologic factors and time to progression and overall survival were evaluated by univariate and multivariable analysis. Fifty-four percent of the @entity43 were found to be diploid, 38% aneuploid, and 8% tetraploid. The S-phase fraction was low (<5%) in 27%, intermediate (5-14.5%) in 47%, and high (>/=14.5%) in 26% of the @entity1 . By univariate analysis overall survival and time to progression were significantly correlated with the S-phase fraction (P = 0.003 and P = 0.003), but not with DNA ploidy (P = 0. 31 and P = 0.51). A DNA index > 1.4 was correlated with poor outcome but the result did not achieve formal statistical significance (P = 0.08 and P = 0.12). A high @entity10359 was a strong predictor of early recurrence, while a low @entity10359 identified @entity1 with a favorable long-term outcome. Other significant predictors of survival were FIGO stage, grade of differentiation, presence of distant @entity3 , @entity41 , lymph node @entity3 , and @entity1 age. In multivariable statistical analysis only FIGO stage, histologic grade, and @entity41 after surgery were independent predictors of overall survival and time to progression.
