Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of XXXX and fructo-oligosaccharides.

@entity948 @entity3456
UNASSIGNED: Owing to applications in the food and nutraceutical industries, inulinases, fructosyltransferases and sucrases have gained considerable attention in recent times. Twenty-five fungal strains were screened for production of these enzymes on three different media formulated using inulin-rich plant extracts prepared from asparagus root, dahlia tuber and dandelion root extract. Culture filtrates of the fungi were examined for hydrolytic activities. Fungi belonging to genus Aspergillus, A. @entity3456 GNCC 2655 (11.3  U/ml), A. awamori MTCC 2879 (8.2  U/ml), A. @entity3456 ATCC 26011 (7.9  U/ml) secreted high titers of inulinase followed by Penicillium sp. NFCCI 2768 (2.6  U/ml) and Penicillium citrinum MTCC 1256 (1.1  U/ml). High sucrase activity was noticed in A. @entity3456 GNCC 2613 (113  U/ml) and A. awamori MTCC 2879 (107.8  U/ml). Analysis of end products of inulinase action by HPLC revealed that most of the enzymes were exo-inulinases liberating @entity948 exclusively from inulin. Five fungi, P. citrinum MTCC 1256, Penicillium rugulosum MTCC 3487, Penicillium sp. NFCCI 2768, A. fumigatus GNCC 1351 and A. @entity3456 ATCC 26011 however, produced a mixture of endo- and exo-inulinases liberating oligosaccharides (GF3 and GF2) along with @entity948 . High inulinase/sucrase yielding strains were evaluated for extracellular and intracellular hydrolytic and transfructosylating activities and intracellular enzyme profiles were found to be considerably different in terms of titers and end products.
