Effect of @entity72948 and intervention of @entity141 -regulated factors on CaN, @entity35897 and p- XXXX expression in neurons.

@entity72948 @entity2308 @entity35 @entity63 @entity3931 @entity205 @entity51 @entity460 @entity141 @entity35897 @entity3390 @entity385
CaN induces the apoptosis in neurons, but the influence of @entity72948 and the intervention of pretreament with @entity141 -regulated factors, such as @entity3931 , @entity3390 and @entity205 , on CaN expression is not clear. We also do not know whether @entity35897 takes part in the induction of @entity63 or induces an expression change of @entity35897 in @entity72948 . So we will discuss the effect of @entity72948 , pretreatment with @entity3931 , @entity3390 and @entity205 on the expression of the CaN, @entity35897 and p- @entity2308 in the hippocampus neurons. In our study, we established @entity35 models including sham, @entity63 , @entity72948 , @entity3931 , @entity3390 and @entity205 . The @entity51 scores were processed. The right hippocampus was removed and stained with TTC, and the volume of @entity385 was calculated. The apoptotic neurons were detected by TUNEL staining. The expressions of CaN, @entity35897 and p- @entity2308 at the protein level were examined by Western blotting, and the transcription of @entity35897 by RT-PCR, respectively. The results showed that the @entity51 scores, the volume of the @entity385 , the numbers of the apoptotic neurons, the protein expression of CaN, @entity35897 and the transcription of @entity35897 were the highest in the @entity63 and @entity3390 groups, there were no difference between the two groups(P>0.05); these factors in @entity72948 group were all lower than those in the @entity63 group(P<0.05); and much lower in the @entity3931 and @entity205 group than those in the @entity72948 group (among them, the volume of the @entity385 in the @entity3931 and @entity205 groups P<0.01, the expression of CaN in @entity3931 group P<0.01, others were P<0.05), but no significant difference existed between the @entity3931 and @entity205 groups(P>0.05). The expression of p- @entity2308 was the lowest in the @entity63 and @entity3390 groups, and there was no difference between the two groups (P>0.05), This factor was higher in @entity72948 group than that in the @entity63 group (P<0.05); it was the highest in the @entity3931 and @entity205 groups among these groups (the @entity3931 group P<0.01, the @entity205 group P<0.05), no significant difference existed between the @entity3931 and @entity205 groups (P>0.05. Continuous @entity63 increases the expression of CaN, and the transcription and the protein expression of @entity35897 . @entity35897 reduces the phosphorylated expression of @entity2308 , ultimately activating apoptosis. @entity72948 inhibits above process and reduces the expression of CaN and @entity35897 , and increases the expression of p- @entity2308 , thereby inhibiting apoptosis in neurons. @entity3931 and @entity205 can reduce the expression of CaN and @entity35897 , and increase the expression of p- @entity2308 , via a moderate increase in the concentration of intracellular @entity460 and inhibition of the activity of CaN; @entity3390 counteracts the effect of @entity72948 .
