Engraftment of T-cell-depleted XXXX bone marrow.

@entity1667 @entity95 @entity205 @entity19574 @entity2132
T cell depletion of donor bone marrow prevents @entity2132 ( @entity2132 ) but increases the risk of rejection. @entity95 bone marrow was T-cell-depleted using the properties of @entity95 T cells to form spontaneous rosettes in the cold and by incubation in @entity1667 (1 mg/ml, 10(6) cells/ml). New Zealand white @entity95 were transplanted with Red Burgundy @entity95 bone marrow from the opposite sex after conditioning with 12 Gy total body irradiation. Untreated animals die of @entity2132 at day 23. Three groups were studied: T cell depletion plus @entity205 (A), T-cell-depleted bone marrow plus irradiated autologous bone marrow plus irradiated donor buffy coat (B), and T-cell-depleted bone marrow plus irradiated autologous bone marrow plus irradiated donor buffy coat plus @entity205 (C). Buffy coat cells irradiated with 15 Gy were given for 5 days. Such cells do not form colonies in culture; however, they are able to release @entity19574 . In group A, 9 of 15 animals rejected, 6 survived for 45 days (median, range 16-249 days). In group B, all 5 of 5 animals died of rejection (median survival time 14 days, range 13-20 days). In group C, 8 of 10 animals did engraft, 4 died of @entity2132 (median survival 48 days, range 26-58 days), and 4 are long-term survivors (greater than 6 months to greater than 1 year), 3 as complete chimeras. We conclude that the combination of irradiated donor buffy coat cells, irradiated autologous bone marrow and @entity205 restores engraftment of T-cell-depleted mismatched bone marrow without losing the benefit of reduced @entity2132 .
