Immunohistochemical, biochemical and immunoelectron microscopic analysis of antigenic proteins on XXXX using monoclonal antibody HISL-19.

@entity1 @entity788 @entity4294 @entity8799 @entity1911 @entity19 @entity5 @entity189 @entity259 @entity7581
The monoclonal antibody HISL-19 was originally generated after immunizing BALB/c @entity19 with @entity1 islet cells. We used this antibody to study a wide variety of neuroendocrine (NE) and non-NE @entity5 by immunohistochemical, immunoelectron microscopic, and biochemical (Western blotting) techniques. Of the @entity259 , HISL-19 specifically immunoreacted with @entity189 of the thyroid (MCT); of the @entity788 , it reacted with @entity4294 and a @entity7581 ; of the @entity5 , it reacted with @entity1911 . HISL-19 showed particularly strong immunoreactivity to a gross granular material at the perinuclear area in the MCT and @entity5 but not in the benign @entity1911 , although the latter cells showed a faint and homogenous positive reactivity in the cytoplasm. The strongly HISL-19-positive material was found to consist of newly synthesized antigenic proteins with a molecular weight between 60 and 65 kilodaltons (kDa) by Western blotting. Immunoelectron microscopic analysis revealed that this antigenic protein was located in the secretory granules that appear markedly in @entity5 , usually located close to the nucleus. Thus, HISL-19 is a useful and specific marker for the immunohistochemical diagnosis of NE cell @entity5 . The specific antigenic proteins of HISL-19 were defined in MCT and @entity5 . These proteins are speculated to be actively synthesized and more highly produced in the secretory granules of @entity5 than benign ones. Thus, a preoperative immunohistochemical study using HISL-19 might be useful for predicting the grade of @entity5 and thus help determine an appropriate operative procedure, in addition to being a useful marker of @entity8799 .
