The effect of XXXX in the thawing solution on the morphology and mobility of frozen equine embryos.

@entity14939 @entity969 @entity12468 @entity653
Seventy-five embryos were collected 6 days after ovulation. Sixty embryos were frozen in straws using @entity653 as the cryoprotectant in an automatic freezer. In Experiment 1 the freezing and thawing media were supplemented with 1.3 g/l @entity12468 ; in Experiment 2 the supplement was 5% FCS. The embryos were thawed for 30 s at +37 degrees C in a waterbath. In Experiment 1 @entity653 was removed from 10 embryos in 6 steps. In 10 other embryos, @entity653 and @entity969 were both removed from the medium in 6 steps. After @entity653 and @entity969 removal, the embryos were stained with @entity14939 ( @entity14939 ) to count the percentage of dead cells. Fluorescent rate (FR) was defined as a ratio of fluorescent area versus total area. Mean (+/- s.d.) FR in this experiment was significantly lower (P<0.01) in embryos thawed with @entity969 (0.28 +/- 0.13) than in embryos thawed with @entity653 alone (0.53 +/- 0.25). In Experiment 2, 40 embryos were frozen and @entity653 , with or without @entity969 , was removed after thawing as for Experiment 1. Ten embryos in both groups were stained with @entity14939 . All the frozen-thawed embryos were transferred nonsurgically to recipient mares. Fourteen fresh embryos were transferred as controls, 7 of which were stained with @entity14939 before transfer. There was no difference in pregnancy rates between @entity14939 -stained versus nonstained embryos, indicating that the staining process had no negative effects on embryonic survival. Insufficient embryos were transferred to be able to demonstrate any difference in pregnancy rates between embryos thawed with or without @entity969 in the medium.
