Immunoenzymometric assay of XXXX kinase with monoclonal antibodies to the MB isoenzyme compared with electrophoresis.

@entity1 @entity3129 @entity908 @entity1188 @entity299
We compare a "second-generation" immunoenzymometric assay (Tandem-E CKMB II) for @entity908 kinase (EC 2.7.3.2) MB with its electrophoretic (Beckman Paragon system) determination. In the former, two monoclonal antibodies are directed against the B and M subunits. We evaluated 502 samples from 253 @entity1 . Precision, linearity, and analytical recovery for both assays were excellent. The two methods correlated well (r = 0.936). The reference interval for individuals with no suspected @entity299 was 0-6.0 micrograms/L; that for non- @entity1188 @entity1 was 0-18.0 micrograms/L. Peak CK-MB values determined by the two assays agreed for 95% of the @entity1 , in terms of exceeding the normal reference interval or not. @entity3129 were 86% (Tandem) and 88% (electrophoresis). The immunoenzymometric assay showed no cross reaction with other CK isoenzymes. Both assay methods performed well in detecting CK-MB, although there were some false positives by both methods, as judged from electrocardiographic results. When total CK for the Tandem assay exceeds 2000 U/L, we recommend calculation of a ratio (CK-MB, micrograms/L:total CK, U/L).
