Targeting of gliadin peptides, XXXX /beta-TCR, and gamma/delta-TCR to Golgi complexes and vacuoles within celiac disease enterocytes.

@entity1 @entity404 @entity1593 @entity8116 @entity784 @entity3505 @entity405
@entity1593 ( @entity1593 ) is characterized by autodestruction of enterocytes after exposure of genetically susceptible individuals to dietary gluten. To define the transport pathways of proteins involved in the celiac immune response, we wished to determine the subcellular compartments of the intestinal mucosa where @entity3505 gliadin peptides colocalize with receptors of T lymphocytes, including alpha/beta-TCR, gamma/delta-TCR, and @entity405 . Semithin and ultrathin frozen section of jejunal biopsies from @entity1593 @entity1 and controls were used to perform immunofluorescence and immunogold labeling as well as in situ hybridization experiments. In @entity1 with active @entity1593 , we detected gliadin peptides in vacuoles and Golgi complexes of enterocytes. @entity405 , alpha/beta-TCR, and gamma/delta-TCR were found in vacuoles and Golgi complexes within these gliadin-containing enterocytes in addition to the surface of intraepithelial and mucosal T lymphocytes. In contrast, we observed that the localization of @entity404 , CD3, @entity784 ( @entity784 ), and @entity8116 ( @entity8116 ) was restricted to lymphocytes in @entity1593 @entity1 . We further detected labeling signals for gliadin peptides, @entity405 /beta-TCR, and gamma/delta-TCR at the basal membrane of enterocytes that were interdigitated by extensions of lymphocytes. In situ hybridization experiments revealed that @entity405 and gamma/delta-TCR were not expressed by @entity1593 enterocytes. We conclude that @entity405 /beta-TCR, and gamma/delta-TCR are targeted to Golgi complexes and vacuoles of small intestinal enterocytes in active @entity1593 . The observed process may be involved in the pathogenesis of @entity1593 enterocytes. We propose a mechanism for the uptake of @entity405 /beta-TCR, and gamma/delta-TCR by the basolateral membrane of small intestinal enterocytes.
