Application of XXXX Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis.

@entity742 @entity26 @entity273 @entity1234 @entity4466 @entity71859 @entity1865
During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive @entity26 species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the @entity1234 ( @entity1865 ). A common method for measuring the concentration of @entity1865 is a @entity4466 reactive substances (TBARS) method. Despite the formation of @entity1865 in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance ( @entity71859 ) spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of @entity273 added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and @entity71859 methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in @entity742 . A measurement using the @entity71859 showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.
