Neuroprotective effects of @entity143 receptor @entity2228 recognition site antagonism: dependence on XXXX concentration.

@entity1026 @entity2227 @entity137 @entity2806 @entity2228 @entity4176 @entity30598 @entity143 @entity880 @entity35 @entity3390
High-affinity @entity143 receptor @entity2228 recognition site antagonists protect brain tissue from @entity2806 . The neuroprotective effect of @entity30598 ( @entity30598 ), a selective @entity143 receptor antagonist with nanomolar affinity for the @entity2228 binding site, was examined in @entity35 cortical mixed neuronal/glial cultures. @entity30598 alone did not alter spontaneous @entity880 dehydrogenase (LDH) release. Treatment with @entity30598 (0.1-10 microM) before 500 microM @entity2227 , 30 microM @entity143 , or 300 microM @entity4176 exposure was found to reduce LDH release in a concentration-dependent fashion. These effects were altered by adding @entity2228 to the medium. @entity2228 (1 mM) partially reversed the effect of @entity30598 on @entity4176 @entity137 . @entity2228 (100 microM-1 mM) completely blocked the effects of @entity30598 on @entity2227 and @entity143 @entity137 . The @entity2228 concentration that produced a half-maximal potentiation of excitotoxin-induced LDH release in the presence of 1.0 microM @entity30598 was similar for @entity2227 and @entity143 (18 +/- 3 and 29 +/- 9 microM, respectively). @entity30598 also reduced @entity4176 @entity137 in cultures treated with @entity3390 . The effects of @entity2228 and @entity30598 on @entity2227 -induced LDH release were consistent with a model of simple competitive interaction for the @entity1026 -insensitive @entity143 receptor @entity2228 recognition site, although nonspecific effects at the @entity4176 receptor may be of lesser importance.
