Expression and activity of ovarian tissue inhibitors of metalloproteinases during pseudopregnancy in the XXXX .

@entity6930 @entity69992 @entity11018 @entity35 @entity8776 @entity11019
The present study examined the role of tissue inhibitors of metalloproteinases (TIMPs) in tissue remodeling that occurs during luteal development and regression throughout pseudopregnancy in the @entity35 . Pseudopregnancy was induced in immature female @entity35 by eCG/hCG priming. Animals (n = 4 per time point) were killed on Days 1, 2, 4, 8, 12, 14, and 16 of pseudopregnancy (post hCG administration), and ovaries were removed and analyzed for metalloproteinase inhibitor activity or @entity6930 , @entity11018 , and @entity11019 mRNA expression. Inhibitory activity was highest in Day-1 samples (41.35 +/- 6.50 inhibitory units), and inhibitor activity significantly decreased (p < 0.05) thereafter to minimal values at Day 12 (8.14 +/- 2.71 inhibitory units). @entity8776 treatment, which inactivates macroglobulin-type inhibitors, revealed that the majority of the inhibitor activity in the Day-1 samples (82.6%) and the Day-16 samples (77.3%) could be attributed to TIMPs. To further distinguish the contribution of each @entity6930 to this activity, Northern analysis for @entity69992 was performed. Analysis of @entity6930 mRNA expression revealed that @entity6930 transcript expression was highest (p = 0.00009) at Day 1, decreased approximately 3- to 20-fold from Days 2 to 12, respectively, and again increased at Days 14-16. However, @entity11018 expression did not change (p > 0.05) over any of the time points studied. In contrast to @entity6930 and @entity11018 expression, @entity11019 mRNA expression was lowest during Days 1 and 2 of pseudopregnancy, increased approximately 4-fold at Day 4, peaked at Day 8, and remained elevated throughout the remainder of pseudopregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)
