Elevated telomerase activity, @entity517 -, and @entity2215 mRNA expression: association with @entity5 progression in XXXX .

@entity1 @entity242 @entity5 @entity517 @entity1758 @entity2215 @entity1821
The role of telomerase activity in @entity5 progression of @entity1821 is not well understood. Therefore, we investigated 72 @entity1821 of different histological subtypes for an association between telomeric lengths and telomerase activity, and assessed the association between the catalytic subunit @entity1 telomerase reverse transcriptase ( @entity2215 ) and its activator @entity517 . Telomeric repeat fragment lengths were determined using radioactive DNA-fingerprint analysis with the telomere-specific probe (TTAGGG)(3), whereas telomerase activity was ascertained using the non-radioactive TRAP-assay. To evaluate the expression of @entity2215 and @entity517 , we applied real-time RT-PCR using a LightCycler. Eight @entity5 were investigated by microdissection. The @entity1758 -proliferation index and @entity2215 and @entity517 protein expression were determined immunohistochemically. Genetic alterations showed a high degree of @entity5 specificity. Highly malignant myxoid/round cell @entity1821 showed the longest telomeres, the strongest telomerase activity, and the highest @entity2215 and @entity517 expression levels compared with the pure myxoid variants (p < 0.001), which are of low malignancy. @entity1821 was characterized by zero or low @entity2215 and @entity517 expression and telomerase activity, but long telomeres, underlining their different pathogenetic pathway. Elevated gene expression was accompanied by protein immunopositivity. @entity1758 -proliferation index did not correlate with other molecular markers. We conclude that @entity2215 and @entity517 expression are associated with telomerase activity in @entity1821 . Elevated @entity2215 and @entity517 expression as well as high telomerase activity play a role in the @entity5 progression of this @entity242 type. Nevertheless, each histological subtype of @entity1821 is defined by a specific molecular pattern. Telomerase activation is the most common pathway in @entity1821 maintaining telomeric length.
