XXXX Adipose Tissue-Derived Mesenchymal Stem Cells Target @entity1313 .

@entity1 @entity650 @entity5737 @entity1313 @entity5 @entity1570 @entity2856 @entity5606 @entity5075 @entity442 @entity10497 @entity1129 @entity687 @entity463 @entity2946 @entity436
UNASSIGNED: In neuro-oncology, the biology of neural stem cells (NSCs) has been pursued in two ways: as @entity5 -initiating cells (TICs) and as a potential cell-based vehicle for gene therapy. NSCs as well as mesenchymal stem cells (MSCs) have been reported to possess @entity5 tropism capacities. However, there is little data on the migratory capacity of MSCs toward @entity1313 -initiating cells (BTICs). This study focuses on the ability of @entity1 adipose tissue derived MSCs (hAT-MSCs) to target BTICs and their crosstalk in the microenvironment. BTICs were isolated from three different types of @entity1313 . The migration capacities of hAT-MSCs toward BTICs were examined using an in vitro migration assay and in vivo bioluminescence imaging analysis. To investigate the crosstalk between hAT-MSCs and BTICs, we analyzed the mRNA expression patterns of cyto-chemokine receptors by RT-qPCR and the protein level of their ligands in co-cultured medium. The candidate cyto-chemokine receptors were selectively inhibited using siRNAs. Both in vitro and in vivo experiments showed that hAT-MSCs possess migratory abilities to target BTICs isolated from @entity2946 , atypical @entity5606 ( @entity1129 /RT) and @entity463 . Different types of cyto-chemokines are involved in the crosstalk between hAT-MSCs and BTICs ( @entity2946 and @entity1129 /RT: @entity436 / @entity1570 , @entity5075 / @entity5737 , @entity10497 / @entity650 and IL8R/ @entity687 ; @entity463 : @entity436 / @entity1570 , @entity10497 / @entity650 , IL8R/ @entity687 and @entity2856 / @entity442 ). Our findings demonstrated the migratory ability of hAT-MSCs for BTICs, implying the potential use of MSCs as a delivery vehicle for gene therapy. This study also confirmed the expression of hAT-MSCs cytokine receptors and the BTIC ligands that play roles in their crosstalk.
