Expression and phosphorylation of XXXX receptors in mitogen-activated peripheral blood lymphocytes.

@entity1 @entity69 @entity865 @entity864 @entity68
Expression of @entity69 receptors of cultured @entity1 lymphocytes has been investigated by using monoclonal antibody (5E9) specific for @entity1 @entity69 receptors. When isolated lymphocytes were cultured in a medium containing fetal calf serum, the biosynthesis of @entity69 receptor was barely detectable. The addition of concanavalin A or human serum to the medium caused a slight stimulation of the biosynthesis. The addition of concanavalin A and human serum in combination caused the highest biosynthetic activity. Appearance of the receptor on the cell surface increased in parallel with the degree of the synthesis. Treatment of concanavalin A- and human serum-treated cells with @entity864 ( @entity865 ) resulted in a marked stimulation of the phosphorylation of the receptor. Enhancement of phosphorylation occurred within 20 min after the addition of @entity865 . The density of the receptor on the cell surface slightly increased upon @entity865 treatment of cells, and the treatment was without effect on @entity68 incorporation from @entity69 into the cells. The density of newly synthesized receptor in @entity865 -treated cells was similar to that in non-treated cells. These results indicated that @entity865 treatment of mitogen-activated @entity1 lymphocytes stimulated the phosphorylation of @entity69 receptors, but @entity865 had no effect on the expression of the receptors thereafter.
