Dynamics of @entity18736 and XXXX expression in the airways of @entity742 following allergen challenge.

@entity1 @entity32 @entity742 @entity565 @entity18730 @entity72209 @entity56 @entity71864 @entity18734 @entity18736 @entity6711
BACKGROUND: @entity18736 and @entity72209 play a critical yet poorly understood role in orchestrating the recruitment and activation of effector cells of the asthmatic response and driving the pathophysiology of @entity56 . The @entity6711 ( @entity6711 ) @entity742 @entity565 model displays many features of the @entity1 condition and is an ideal model to further elucidate the involvement of these critical Th2 cytokines. We hypothesized that airway exposure to @entity6711 allergen would induce or elevate the expression profile of @entity18736 and @entity72209 during the allergic airway response in this large animal model of @entity565 . METHODS: Bronchoalveolar lavage (BAL) samples were collected from saline-  and @entity6711 ( @entity6711 )-  challenged lung lobes of sensitized @entity742 from 0 to 48  h post-challenge. BAL cytokines ( @entity18736 , @entity72209 , @entity18730 , @entity71864 , @entity18734 ) were each measured by ELISA. @entity18736 and @entity72209 expression was assessed in BAL leukocytes by flow cytometry and in airway tissue sections by immunohistology. RESULTS: @entity18736 and @entity72209 were increased in BAL samples following airway allergen challenge. @entity6711 challenge resulted in a significant increase in BAL @entity18736 levels at 4  h compared to saline-challenged airways, while BAL @entity72209 levels were elevated at all time-points after allergen challenge. @entity18730 levels were maintained following @entity6711 challenge but declined after saline challenge, while @entity6711 administration resulted in an acute elevation in @entity71864 at 4  h but no change in @entity18734 levels over time. Lymphocytes were the main early source of @entity18736 , with @entity18736 release by alveolar macrophages (AMs) prominent from 24  h post-allergen challenge. @entity72209 producing AMs were increased at 4 and 24  h following @entity6711 compared to saline challenge, and tissue staining provided evidence of @entity72209 expression in airway epithelium as well as immune cells in airway tissue. CONCLUSION: In a @entity742 model of @entity56 , airway @entity32 is accompanied by the temporal release of key cytokines following allergen exposure that primarily reflects the Th2-driven nature of the immune response in @entity565 . The present study demonstrates for the first time the involvement of @entity18736 and @entity72209 in a relevant large animal model of @entity56 .
