Loss of fragile @entity3793 triad gene expression in advanced XXXX is consequent to allelic loss at 3p14 locus and promoter methylation.

@entity3793 @entity2 @entity21900
The fragile @entity3793 triad ( @entity21900 ) gene located at the 3p14.2 locus plays an important role in the pathogenesis of @entity2 . The objective of this study was to analyze loss of heterozygosity and @entity21900 gene methylation status and correlate them to @entity21900 expression. Bronchoscopically obtained lung biopsies from 30 cases of histologically proven @entity2 in stage III were assessed for the alterations in the @entity21900 gene. @entity21900 protein expression was determined by immunohistochemistry, and transcript levels were determined by reverse transcription-PCR. Microsattelite alterations and methylation status of the @entity21900 gene promoter was determined by PCR. Loss of heterozygosity at the 3p14 locus was observed in all the 30 cases at least by one of the three microsatellite polymorphic markers. The @entity21900 gene promoter showed complete methylation in 37% cases and partial methylation in 47% cases, and 16% cases showed no promoter methylation. @entity21900 full-length coding region (exons 5-9) transcripts were present in eight cases (26.6%), and aberrant transcripts were additionally seen in four cases. Loss of @entity21900 mRNA expression correlated to @entity21900 promoter methylation but not to loss of heterozygosity at the 3p14 locus. There was a strong correlation between the expression of @entity21900 at the transcript and protein level. The apoptotic index estimated by the terminal deoxynucleotidyl transferase-mediated nick end labeling assay was significantly correlated to the @entity21900 protein expression. The results of this study indicate that in locally advanced @entity2 , there is frequent loss of @entity21900 expression, and methylation of the @entity21900 gene promoter is an important mechanism of its inactivation.
