A comprehensive transcriptome analysis of silique development and dehiscence in XXXX and Brassica integrating genotypic, interspecies and developmental comparisons.

@entity71798 @entity7214 @entity71796 @entity4335 @entity1786 @entity958 @entity29399 @entity39121 @entity13344 @entity71797
Asynchronous flowering of @entity4335 (canola) leads to seeds and siliques at varying stages of maturity as harvest approaches. This range of maturation can result in premature silique dehiscence (pod shattering), resulting in yield losses, which may be worsened by environmental stresses. Therefore, a goal for canola crop improvement is to reduce shattering in order to maximize yield. We performed a comprehensive transcriptome analysis on the dehiscence zone (DZ) and valve of @entity958 and Brassica siliques in shatter resistant and sensitive genotypes at several developmental stages. Among known @entity958 dehiscence genes, we confirmed that homologs of @entity71796 , @entity29399 , @entity71797 , @entity71798 and IND were associated with shattering in @entity13344 and @entity4335 . We noted a correlation between reduced pectin degradation genes and shatter-resistance. Tension between lignified and non-lignified cells in the silique DZ plays a major role in dehiscence. Light microscopy revealed a smaller non-lignified separation layer in relatively shatter-resistant @entity13344 relative to @entity4335 and this corresponded to increased expression of peroxidases involved in @entity39121 polymerization. Sustained repression of auxin biosynthesis, transport and signaling in @entity13344 relative to @entity4335 may cause differences in dehiscence zone structure and cell wall constituents. Tension on the dehiscence zone is a consequence of shrinkage and loss of flexibility in the valves, which is caused by senescence and desiccation. Reduced shattering was generally associated with upregulation of ABA signaling and down-regulation of @entity1786 and @entity7214 signaling, corresponding to more pronounced stress responses and reduced senescence and photosynthesis. Overall, we identified 124 cell wall related genes and 103 transcription factors potentially involved in silique dehiscence.
