Establishment, growth properties, and morphological characteristics of permanent XXXX small cell lung cancer cell lines.

@entity1 @entity2 @entity4153
Cell lines from @entity4153 were established with a success rate of 43% from different metastatic sites of treated and untreated @entity1 . All 6 @entity4153 cell lines grew as floating cell aggregates without substrate adherence. The degree of aggregation ranged from very tight spheroids to very loose sheets and chains. This gross morphological property showed a striking correlation to the PDT, with short PDTs in loose growing cell lines and long PDTs in tight growing cell lines. Cell size and nuclear features, i.e., chromatin pattern and nucleolar prominence, also seemed to correlate with the PDT and gross morphology. All @entity4153 cell lines had dense core granules by electron microscopical examination. Several different serum-free and serum-supplemented growth media were tested for their feasibility in establishing and permanently growing @entity4153 . Serum-free SIT medium and SIT2.5 medium provided the best results in liquid culture. For semisolid @entity4153 cultivation, R 10 medium was superior to all other media tested. These cell lines are currently under intensive biochemical, molecular biological, and cytogenetical investigation in different laboratories and thus provide a tool for studying the biology of @entity2 .
