Adiponectin inhibits osteoclastogenesis and bone resorption via XXXX -mediated suppression of @entity1994 .

@entity5272 @entity35364 @entity851 @entity101 @entity19 @entity72918 @entity5271 @entity1994 @entity25314 @entity25433
Adiponectin is an adipokine playing an important role in regulating energy homeostasis and insulin sensitivity. However, the effect of adiponectin on bone metabolism shows contradictory results according to different research studies. In this study femurs were isolated from genetically double-labeled mBSP9.0Luc/b-ACT-EGFP @entity19 and were transplanted into adiponectin knock-out @entity19 or wild type @entity19 to investigate the effect of temporary exposure to @entity851 and metabolism. We found that the growth of bone explants in adiponectin knock-out @entity19 was significantly retarded. Histological analysis, microcomputed tomography analysis, and @entity5272 -resistant acid phosphatase staining revealed reduced trabecular bone volume, decreased cortical bone, and @entity101 in bone explants in adiponectin knock-out @entity19 . We then found that adiponectin inhibits @entity5271 -induced osteoclastogenesis from RAW264.7 cells and down-regulates @entity5271 -enhanced expressions of osteoclastogenic regulators including @entity25314 , @entity25433 , @entity35364 , and @entity5272 -resistant acid phosphatase. Adiponectin also increases osteoclast apoptosis and decreases survival/proliferation of osteoclast precursor cells. Using siRNA specifically targeting @entity72918 , the first identified adaptor protein of adiponectin signaling, we found that the inhibitory effect of adiponectin on osteoclasts was induced by @entity72918 -mediated down-regulation of @entity1994 activity. In addition, overexpression of @entity1994 successfully reversed adiponectin-induced inhibition in @entity5271 -stimulated osteoclast differentiation. In conclusion, adiponectin is important in maintaining the balance of energy metabolism, inflammatory responses, and bone formation.
