Rapid detection of @entity470 mutations in XXXX using real-time polymerase chain reaction with TaqMan-MGB probes.

@entity1 @entity470 @entity5 @entity1149 @entity3 @entity669 @entity420
We investigated somatic mutations of the @entity470 gene in @entity1149 tissue and their detection using real-time polymerase chain reaction with TaqMan-MGB probes. METHODS: The DNA was extracted from surgically resected @entity1149 specimens. Genes encoding for @entity470 @entity669 (exons 18, 19, and 21) were amplified by nested polymerase chain reaction, sequenced, and analyzed by chromatograms with manual review. TaqMan-MGB probes were designed to detect the @entity470 gene mutations in the @entity5 tissues using real-time polymerase chain reaction. RESULTS: Somatic point mutations and deletions were identified in the @entity669 kinase domain of the @entity470 gene in 21 of 80 @entity1149 @entity1 , including 13 @entity1 with deletion mutations occurring in exon 19 and 8 @entity1 with point mutations occurring in codon 858 (exon 21). The results from real-time polymerase chain reaction with TaqMan-MGB probes were completely consistent with sequencing outcomes. Both the sensitivity and specificity for detecting the @entity470 gene mutations using real-time polymerase chain reaction with TaqMan-MGB probes were 100%. The mutation incidence was significantly higher in female @entity1 , nonsmokers, and @entity1 with @entity420 than in male @entity1 , smokers, and those with nonadenocarcinomas (P < 0.05). The mutations were not related to @entity1 's age or @entity5 nodal @entity3 staging. CONCLUSIONS: Somatic mutations of the @entity470 gene that develop in @entity1149 @entity1 are more common in female @entity1 , nonsmokers, and @entity1 with @entity420 . Real-time polymerase chain reaction using TaqMan-MGB probes is effective, simple, and fast in the detection of @entity470 gene mutations.
