Expression of proapoptotic XXXX and @entity876 genes and antiapoptotic @entity588 gene in MCF-7 and T-47D @entity5 cell cultures of the mammary gland after a photodynamic therapy with photolon.

@entity1 @entity589 @entity588 @entity0 @entity5 @entity876
BACKGROUND: @entity0 is the most common @entity5 in @entity1 in the whole world. Despite significant developments in the early diagnosis of @entity0 , there is no effective method which would assure total recovery of the @entity1 . Currently available clinical data and laboratory tests indicate a possibility to introduce photodynamic therapy (PDT) to the supplementary treatment of @entity0 . OBJECTIVES: The aim of this study was to assess the influence of PDT with Photolon as a photosensibilizator on the expression of apoptosis associated genes ( @entity588 , @entity589 , @entity876 ) in @entity1 @entity0 cell lines, preceded by assessment of survivorship and proliferative activity in the tested cells after PDT. MATERIAL AND METHODS: In the present study @entity1 @entity0 cell lines MCF-7 and T-47D were used. Photolon (chlorin e6 complex: PVP 1:1) was used as a photosensitizer. Assessments of survivorship and proliferative activity of cells under the influence of PDT (WST-1 test) were conducted along with the expression of selected genes involved in the process of apoptosis: @entity588 , @entity589 , @entity876 (RT-QPCR). RESULTS: PDT limited both survivorship and proliferative activity of @entity0 cells in the two tested lines. In case of T-47D cell line was found increase of @entity589 and @entity588 genes expression after PDT and sustained activity of @entity876 gene. Conversely, in MCF-7 cell line a decrease in expression was found for both @entity589 and @entity876 genes, but also an increase of @entity588 gene expression. CONCLUSIONS: A progressing decrease (24, 48 and 72 h after PDT) in the count of culture cells, which suggests the occurrence of apoptosis initiated by a photodynamic reaction with simultaneous increase of @entity588 / @entity589 index, indicates activation of a different endogenous apoptosis pathway than the one examined, namely pointing to suicidal death of cells after PDT.
