Validating polymerase chain reaction for detecting HPV in XXXX .

@entity1 @entity7063 @entity8286 @entity281 @entity3146
OBJECTIVE: To validate polymerase chain reaction (PCR) analysis for detecting HPV in Mexican @entity1 with @entity3146 , grade 2 and 3 (CIN 2/3) versus histologic evidence. STUDY DESIGN: A diagnostic test study was carried out. A sample of 25 selected @entity1 who were diagnosed by histology as having CIN 2/3 was analyzed. Biopsies were examined for @entity281 using light microscopy. The histologic criteria used for @entity281 included koilocytosis, @entity7063 cells, bi/multinucleation, and @entity8286 . PCR was performed on each sample using commercial probes (MY09 and MY11), and then HPV typing was carried out by restriction fragment length polymorphism analyses. RESULTS: PCR revealed that 88% (22/25) of the @entity1 were HPV positive (19 high risk and 3 low risk). In contrast, histology revealed that 28% (7/25) of the @entity1 were HPV positive. The number of @entity1 infected with HPV was 3.14 times (88/28) more frequently detected with PCR procedure than with the histology. Using PCR as the gold standard, 4 values (true positive, false positive, false negative and true negative) were obtained (7, 0, 15 and 3), and histology had a sensitivity, specificity, and positive and negative predictive values of .32, 1.00, 1.00 and .17, respectively. There was a correlation between low-risk and high-risk for PCR (chi 2 with Yates correction = 6.32, P = .012). CONCLUSION: PCR is a powerful tool for the early detection of @entity281 and is independent of histologic criteria.
