XXXX specific cytotoxic IgG-autoantibodies against @entity694 cells.

@entity1 @entity137 @entity31 @entity3407 @entity694 @entity30
Serum antibodies cytotoxic to the @entity694 cell line RPMI 4788 were studied in 42 @entity1 with @entity31 , 61 @entity1 with @entity30 , 27 @entity1 with other inflammatory diseases (disease-controls) and 22 healthy controls. @entity137 of antibodies towards RPMI 4788 was studied by means of a @entity3407 release assay using peripheral blood mononuclear leucocytes of healthy subjects as effector cells. Using a four hour antibody dependent cell mediated cytotoxicity assay sera from 29% of @entity31 patients contained antibodies cytotoxic for the target, while only 3% of the @entity30 @entity1 and 6% of the disease controls and non of the healthy controls were positive. When an 18 hour assay was applied, however, not only 40% of @entity31 @entity1 , but also 14% of @entity30 @entity1 and 21% of disease controls were found positive. The reactive antibody in the four hour assay was mainly of the IgG class, which points at a classical antibody dependent cell mediated cytotoxicity mechanism. In the 18 hour cytotoxic assay IgG and particularly IgM antibodies were found to be reactive. This suggests that in the latter case other cellular cytotoxic mechanism might be involved. There was a significant inverse correlation between the appearance of the @entity31 restricted IgG-anticolon epithelial cell antibodies (four hour assay) and the disease activity (p less than 0.01). Absorption studies showed that the reactive antigen is not specific for @entity31 colonic tissue, but is similarly found in @entity30 , and to a lower extent in normal bowel, liver and kidney. The reactive antigen, however, could not be detected in brain and lymphoblastoid cells.
