Diagnostic potential of XXXX expressing epithelial cells in blood of @entity263 @entity1 .

@entity1 @entity10982 @entity263 @entity1486
PURPOSE: @entity1486 (PSA) test has become a widely used screening test in @entity263 ( @entity10982 ). However, low specificity of serum PSA leads to many false-positive and false-negative results and clinical uncertainty. Development of @entity10982 -specific diagnostic and prognostic markers is needed. Detection of circulating PSA-expressing cells (CPECs) in blood and bone marrow of @entity10982 @entity1 has potential in molecular diagnosis and prognosis. Our novel observations of the frequent presence of CPECs in @entity10982 @entity1 with organ-confined disease by reverse transcription (RT)-PCR-PSA assay in epithelial cells enriched from peripheral blood (ERT-PCR/PSA) have led us to test the hypothesis that CPECs have diagnostic potential for @entity10982 . EXPERIMENTAL DESIGN: Epithelial cells from peripheral blood of radical prostatectomy @entity1 or prostate biopsy @entity1 were isolated using antiepithelial cell antibody, Ber-EP4-coated magnetic beads, and total RNA specimens from these cells were analyzed for PSA expression by RT-PCR. RESULTS: Peripheral blood specimens of 108 of 135 (80.0%) @entity10982 @entity1 were positive in ERT-PCR/PSA assay. Peripheral blood specimens from 45 control @entity1 were virtually negative (97.8%). In the blinded investigation, 84 @entity1 who had biopsy for suspicion of @entity10982 were evaluated by ERT-PCR/PSA assay. Eighteen of 22 (81.8%) @entity1 with biopsy-proven @entity10982 were positive, and 54 of 62 (87.1%) @entity1 with biopsy negative for @entity10982 were negative in this assay (P < 0.001). CONCLUSIONS: Our study provides intriguing novel results showing that the majority of @entity1 with clinically organ-confined @entity10982 contain CPECs. Strong concordance between the biopsy results and ERT-PCR/PSA assay (sensitivity 81.8%; specificity 87.1%) suggests a potentially new diagnostic application of this type of assay in @entity10982 diagnosis.
