Effect of low intensity pulsed ultrasound on expression of @entity20688 in serum and expression of @entity28477 in articular cartilage of XXXX with @entity626 .

@entity20688 @entity626 @entity571 @entity28477 @entity95
OBJECTIVE: To study the effect of low intensity pulsed ultrasound (LIPUS) on the expression of @entity20688 ( @entity20688 ) in the serum and expression of @entity28477 ( @entity28477 ) in the articular cartilage cells of @entity95 with @entity626 ( @entity571 ). METHODS: Inner patellar ligament defect method was used to establish the model of knee @entity571 . Four weeks after the modeling, the arterial blood was drawn from the ear of each @entity95 , while ELISA was employed to detect the expression of @entity20688 in the serum. The chondrocytes were separated from animals in each group and then cultured in  vitro. All @entity95 were divided into control group, @entity571 model group and @entity571  +  LIPUS group. Cells in the control and @entity571 groups were not treated, while cells in the @entity571  +  LIPUS group were treated with LIPUS (40  mW/cm(2), 1 time/day). Cells were collected 7  d later and the RNA and total protein were extracted respectively. Real-time PCR and Western blotting were employed to analyze the expression of @entity28477 in chondrocytes at the mRNA and protein level, respectively. RESULTS: The success rate of establishment of @entity571 model was 83%. The results of ELISA showed that the content of @entity20688 in the serum of animals with @entity571 was 22.3%, lower than the one in the control group (P  <  0.05). Compared with the normal control group, the expression of @entity20688 in the @entity571 model group was significantly increased, while the expression of @entity28477 was significantly increased (P  <  0.05). After the stimulation of LIPUS, the expression of @entity20688 and @entity28477 was close to the one in the normal control group. CONCLUSIONS: The inner patellar ligament defect method is a mature method to establish the @entity95 @entity571 model, with high success rate. The expression of serum @entity20688 in the @entity571 model group is significantly decreased. LIPUS can up-regulate @entity20688 and down-regulate @entity28477 .
