Time course differential gene expression in response to @entity18723 subclinical XXXX .

@entity1707 @entity18723 @entity5653 @entity281
This study was aimed at characterizing the potential differences in gene expression in piglets inoculated with @entity18723 ( @entity18723 ), the essential causative agent of postweaning @entity5653 . Seven-day-old caesarean-derived, colostrum-deprived piglets were distributed into two groups: control (n = 8) and @entity1707 inoculated with 10(5.2) TCID(50) of the Burgos @entity18723 isolate (n = 16). One control and three inoculated @entity1707 were necropsied on days 1, 2, 5, and 8 post- @entity281 (p.i.). The remaining @entity1707 (four of each group) were sequentially bled on days 0, 7, 14, 21, and 29 p.i. (necropsy). Total RNA from the mediastinal lymph node (MLN) and lysed whole blood (LWB) samples were hybridized to Affymetrix Porcine GeneChip. Forty-three probes were differentially expressed (DE) in MLN samples (FDR < 0.1, fold change > 2) and were distributed into three clusters: globally down-regulated genes, and up-regulated genes at early (first week p.i.) and late (day 29 p.i.) stages of @entity281 . In LWB samples,maximal differences were observed at day 7 p.i., with 54 probes DE between control and inoculated @entity1707 . Main Gene Ontology biological processes assigned to upregulated genes were related to the immune response. Six common genes were found in both types of samples, all of which belonged to the interferon signaling antiviral effector pathway. Down-regulated genes were mainly related to cell adhesion and migration in MLN, and cellular organization and biogenesis in LWB. Microarray results were validated by quantitative real-time PCR. This study provides, for the first time, the characterization of the early and late molecular events taking place in response to a subclinical @entity18723 @entity281 .
