Triple therapy with @entity2390 , galanin and @entity750 induces XXXX and increases apoptosis of a @entity35 @entity694 .

@entity39 @entity19 @entity5 @entity16424 @entity750 @entity2390 @entity35 @entity694 @entity420
A @entity35 colonic @entity420 was implanted subcutaneously (s.c.) in @entity19 . After 7 days, the animals were divided into different groups. Two groups received subcutaneous injections twice daily with 3 or 6 micro g/kg body weight @entity2390 , @entity16424 and @entity750 . Three groups were respectively treated with 20, 30, and 40 micro g/kg body weight of the previously mentioned bioactive substances. Control group received only saline solution in the same fashion as treated animals. The treatment lasted for 5 days. The @entity5 volume and weight, the relative density of blood vessels, of @entity5 @entity39 tissue, of apoptotic nuclei and of proliferating nuclei were measured. Apoptosis was detected by in situ labelling of nuclear DNA fragmentation according to TUNEL method, and proliferation by immunocytochemistry. Morphometry was done with the classical stereological point-counting method. Food consumption, animal weight, faeces weight and its water content were measured for 3 days before and after treatment. Triple therapy with 3 and 6 micro g/kg body weight had no effect on any of the parameters measured, except in reducing the relative volume density of @entity5 blood vessels. Treatment with 20, 30 and 40 micro g/kg body weight of the previously mentioned bioactive substances reduced the @entity5 volume, the relative volume density of blood vessels and increased the relative volume density of @entity39 tissue and of apoptotic nuclei (in the 20 micro g group). However, there was no difference between treated @entity19 and controls regarding the relative volume density of proliferating nuclei. There was no statistical difference between treated animals regarding food consumption, body weight, faeces weight and its water content before and during treatment. The present study confirms that triple therapy with @entity2390 , galanin and @entity750 causes regression of a @entity35 @entity694 . It further showed that optimum treatment dose is 20 micro g/kg body weight of each bioactive substance. Moreover, this therapy regime does not show apparent side effects in the experiments carried out on @entity19 .
