Aldose reductase inhibitor counteracts the attenuated adhesion of XXXX corneal epithelial cells induced by high @entity413 through modulation of @entity12791 expression.

@entity1 @entity12791 @entity3650 @entity10916 @entity413 @entity8371
AIMS: We investigated the preventive effect of @entity3650 reductase inhibitor ( @entity10916 ) on the adhesion of SV40-transformed @entity1 corneal epithelial cells (HCECs) exposed to high @entity413 , and the underlying mechanism focusing on the role of @entity12791 . METHODS: HCECs were cultured in medium containing a normal (5.5 mM) or high (31.2 mM) concentration of @entity413 in the presence or absence of @entity10916 , fidarestat. Cell attachment ability was evaluated by short-term adhesion assay. The levels of intracellular @entity8371 were measured by liquid-gas chromatography. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR), and Western blotting were used to determine the expression levels. RESULTS: Decreased attachment activity and increased accumulation of @entity8371 induced by exposure to high @entity413 were abrogated by @entity10916 . Supply of recombinant @entity12791 decreased integrin alpha3beta1-expression and cell adhesion. The expression level of @entity12791 was enhanced at both protein and mRNA levels by exposure to high @entity413 , while that of integrin alpha3beta1 was decreased at the protein level, but remained unchanged at the mRNA level. These alterations in the expression levels of @entity12791 and integrin alpha3beta1 were normalized by @entity10916 . CONCLUSIONS: @entity10916 counteracts the decreased adhesion of HCECs induced by high @entity413 exposure, through the modulation of the expression of @entity12791 .
