Flow cytometric analysis of nuclear DNA from @entity1636 . A retrospective study using XXXX -embedded tissue.

@entity2007 @entity5 @entity189 @entity10163 @entity982 @entity1636 @entity3 @entity204
Nuclear DNA content of @entity982 -embedded tissue from 48 @entity1636 , 18 histologically normal control adrenal glands, and five @entity10163 was analyzed retrospectively using flow cytometry. Aneuploidy was compared with morphologic criteria as a predictor of recurrence. All 18 controls, five hyperplastic glands, and 39 @entity5 were diploid. Nine @entity5 were aneuploid. Compared with their diploid counterparts, @entity5 were more likely to weigh more than 50 g (P less than 0.0001) and to have three or more histologic features of @entity189 (P less than 0.0001). Thirty-six @entity5 were followed clinically for at least 2 years (range 24 to 120 months, mean = 64.6 months) or until local recurrence, @entity3 , or @entity204 . Five were clinically malignant. @entity5 which recurred or metastasized were more apt to be aneuploid (P less than 0.005) than those showing no evidence of further disease during the follow-up period. They were also more likely to weigh more than 50 g (P less than 0.005) and to have three or more histologic features of @entity189 (P less than 0.0025). However, neither @entity2007 , large size, nor unfavorable histology result was a consistent feature in every malignant @entity5 . Flow cytometric DNA content analysis appears to be as effective a predictor of clinical outcome as size and histology and may be of particular value when the morphologic features are ambiguous.
