Inhibitory effects of @entity14216 , a Kv7 channel activator, on mechanosensitive primary bladder afferent activities and @entity146 in XXXX .

@entity1494 @entity146 @entity4774 @entity972 @entity35 @entity14216
AIMS: Kv7 voltage-gated @entity972 channels have been suggested to modulate mechano-afferent transduction and nociception in the bladder. We investigated the effects of @entity14216 , a Kv7 channel activator, on @entity1494 ( @entity1494 ), and single-unit afferent activities (SAAs) of the primary bladder mechanosensitive afferent nerve fibers in @entity4774 -anesthetized @entity35 . In addition, the effects of pretreatment with @entity14216 on the @entity146 provoked by an intravesical instillation of resiniferatoxin (RTX) were evaluated in the conscious condition. METHODS: Female @entity35 were used. Under @entity4774 anesthesia, saline was instilled into the bladder until @entity1494 were induced reproducibly. Then, the effects of intravenous, cumulative administrations of @entity14216 (0.1-3   mg/kg) or vehicle (saline) on @entity1494 were assessed. In separate animals, SAAs of A  - and C-fibers were identified by electrical stimulation of the pelvic nerve and by @entity1494 with saline. After baseline recording, vehicle or @entity14216 (0.01-1   mg/kg) was administered intravenously and further recordings were performed. Under pretreatment with vehicle or @entity14216 (3   mg/kg intraperitoneally), the frequencies of lower abdominal licking and freezing were counted and scored as the bladder @entity146 induced by intravesical RTX instillation (3     M, 0.3   ml). RESULTS: @entity14216 dose-dependently decreased both the frequency and the amplitude of @entity1494 and SAAs of both A  - and C-fibers. The effect on @entity1494 was more potent on the frequency than the amplitude. @entity14216 inhibited the RTX-induced abdominal licking, but not freezing. CONCLUSION: Kv7 channels are likely to be implicated in inhibition of bladder mechano- and nociceptive sensory transduction. Neurourol. Urodynam.    2015 Wiley Periodicals, Inc.
