Effects of breed, parity, and @entity1501 supplement on the expression of XXXX metabolism genes in endometrial and embryonic tissues from sows in early pregnancy.

@entity1501 @entity1707 @entity1619 @entity2228 @entity535 @entity563
@entity1501 and @entity2228 are factors of great importance in early gestation. In sows, @entity1501 supplement can increase litter size through a decrease in embryonic mortality, while @entity2228 , the most abundant @entity535 in the sow oviduct, uterine, and allantoic fluids, is reported to act as an organic osmoregulator. In this study, we report the characterization of cytoplasmic @entity563 hydroxymethyltransferase (cSHMT), T-protein, and @entity1619 ( @entity1619 ) mRNA expression levels in endometrial and embryonic tissues in gestating sows on Day 25 of gestation according to the breed, parity, and @entity1501 + @entity2228 supplementation. Expression levels of cSHMT, T-protein, and @entity1619 mRNA in endometrial and embryonic tissues were performed using semiquantitative reverse transcription-polymerase chain reaction. We also report, for the first time, an alternative splicing event in the porcine T-protein gene. Results showed that a T-protein splice variant, @entity1619 , is present in all the tested sow populations. Further characterizations revealed that this T-protein splice variant contains a coding intron that can adopt a secondary structure. Results demonstrated that cSHMT mRNA expression levels were significantly higher in sows receiving the @entity1501 + @entity2228 supplementation, independently of the breed or parity and in both endometrial and embryonic tissues. Upon receiving the same treatment, the @entity1619 and T-protein mRNA expression levels were significantly reduced in the endometrial tissue of Yorkshire-Landrace sows only. These results indicate that modulation of specific gene expression levels in endometrial and embryonic tissues of sows in early gestation could be one of the mechanism involved with the role of @entity1501 on improving @entity1707 reproduction traits.
