Identification of XXXX genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic.

@entity1 @entity18276 @entity21786 @entity2069 @entity12732 @entity69881 @entity25974
INTRODUCTION: During 2008-2012, a total of 466 ticks Ixodes ricinus removed from @entity1 were collected and tested for the presence of @entity2069 sensu lato (Bbsl). Ticks were collected in all districts of the South Moravian region of the Czech Republic (CZ). OBJECTIVE: The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. MATERIAL AND METHODS: DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). Detection of spirochetes was carried out by RealTime PCR kit EliGene @entity12732 (Elisabeth Pharmacon). Finally, all the positive samples were sequenced on an ABI 3130 Genetic Analyzer (Life Technologies) and identified in the BLAST (NCBI) database. RESULTS: A total positivity of the samples was 26%. For the first time in the Czech Republic, 5 of the isolated strains were genotyped as @entity69881 (7.1%). Other representatives of Bbsl were also observed: @entity21786 (70.0%), @entity25974 (10.0%), @entity18276 (8.6%), and @entity2069 s. s. (4.3%). CONCLUSION: A general view of the spreading of Bbsl in the South Moravian region was demonstrated. The most interesting result of the study is the finding of @entity69881 for the first time in this region.
