@entity6579 regulates XXXX microvascular endothelial cell expression of chemokines.

@entity1 @entity32 @entity5737 @entity6579 @entity4286 @entity310 @entity3963 @entity584 @entity2306 @entity2842 @entity3900 @entity4285 @entity3766 @entity15002 @entity687 @entity1366
The role of @entity6579 ( @entity15002 ) in the induction of @entity2842 , @entity687 and @entity5737 , which are chemotactic factors to monocytes, neutrophils and lymphocytes, respectively, by @entity1 vascular endothelial cells (EC), was examined. @entity15002 induced the expression of @entity2842 and @entity687 in a concentration- and time-dependent manner in microvascular EC (MVEC) and in large vessel EC from aorta, pulmonary artery and umbilical vein. @entity15002 also induced @entity5737 in MVEC but not in large vessel EC. Signaling pathways responsible for @entity15002 induction of chemokines were examined in MVEC. @entity15002 and @entity310 , a cytokine secreted in sites of @entity32 , additively stimulated @entity5737 expression. @entity15002 did not augment @entity310 induction of @entity2842 or @entity687 . A platelet-activating factor receptor antagonist ( @entity3766 ) failed to block @entity15002 induction of MVEC chemokines, but the G(i)-protein inhibitor pertussis toxin partially blocked @entity15002 induction of @entity5737 and @entity687 . @entity15002 activated multiple kinases in MVEC; it increased the phosphorylation of @entity1366 , @entity584 and @entity3963 MAP kinase in a time-dependent manner. An inhibitor of the MAPK/ERK pathway, @entity4286 , blocked the phosphorylation of @entity1366 and @entity5737 induction by @entity15002 , but augmented @entity687 induction. @entity2306 , a specific inhibitor of phosphoinositide 3 kinase (PI3 kinase), blunted the phosphorylation of @entity584 and inhibited @entity15002 induction of @entity5737 more strongly than @entity687 . Inhibition of @entity3963 MAP kinase pathway by @entity4285 also blocked @entity15002 -induced expression of @entity687 and @entity5737 . Our results suggest that @entity15002 induction of chemokines in MVEC is distinct from that in large vessel EC, and required the activities of MAP kinases and PI3 kinase for the induction of @entity5737 and @entity687 . We speculate that the presence of @entity15002 , a bioactive lipid product of @entity3900 ( @entity3900 ) and a constituent of oxidized low-density lipoprotein, can differentially influence the chemotaxis of particular leukocyte subpopulations during @entity32 .
