Migration and differentiation of adult XXXX subventricular zone progenitor cells transplanted into the adult @entity35 striatum.

@entity35 @entity1603 @entity19744
Adult brain subventricular zone progenitor cells undergo neurogenesis in the olfactory bulb. We tested the hypothesis that cultured adult subventricular zone progenitor cells migrate and differentiate into neurons when transplanted into the adult striatum. Cells in the adult @entity35 subventricular zone were isolated and cultured for 8 days in medium containing basic fibroblast growth factor. These cells proliferated as assayed by @entity1603 immunostaining, and the majority of them were neuron-specific class III beta-tubulin (TuJ1) immunoreactive at 8 days of culture. These cultured cells were labeled in vitro with @entity1603 or with lipophilic dye-coated particles and were transplanted into the adult @entity35 striatum. Twenty-eight days after transplantation, the cells migrated 0.5-1.5 mm from the midline of the graft to the surrounding host striatum. Migration of grafted cells in the host striatum was also detected on magnetic resonance imaging in living @entity35 . Morphological analysis revealed that many of these migrated cells exhibited multibranched processes from the cell soma resembling host medium-size striatal projection neurons. Only a few astrocyte-like cells were detected. Double immunostaining showed that many @entity1603 immunoreactive cells were @entity19744 or immunoreactive with a mouse monoclonal antibody against neuronal nuclear protein, whereas only a few @entity1603 immunoreactive cells had glial fibrillary acidic protein immunoreactivity. Morphology of @entity1603 and @entity19744 immunoreactive cells was similar to those of host @entity19744 immunoreactive cells. These results suggest that transplanted cultured adult subventricular zone progenitor cells can migrate and differentiate in response to guidance cues within the adult striatum.
