[Effects of @entity3413 on bone formation relative factors of trabecular bone and osteogenic differentiation of bone marrow mesenchymal stem cells in young XXXX ].

@entity16835 @entity6037 @entity60738 @entity12480 @entity3413 @entity21408 @entity72655 @entity2095 @entity35 @entity731 @entity6667
OBJECTIVE: @entity3413 has been reported to be effective on stimulation of bone formation. To investigate the effects of @entity3413 on bone formation relative factors of proximal tibia trabecular bone and on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). METHODS: Fourty 1-week-old male @entity35 were divided randomly into 2 groups, 20 @entity35 per group. @entity35 in experimental group received subcutaneous injection of @entity3413 [(5 mg/(kg x d)], and the @entity35 in control group received injection of normal saline at the same dose. The expressions of @entity2095 ( @entity2095 ), @entity12480 ( @entity12480 ), and @entity6667 ( @entity6667 ) of trabecular bone were analyzed in the tibia by immunohistochemical staining at 1 and 3 weeks after injection. BMSCs from the @entity35 femur at 1 and 3 weeks after injection were cultured under condition of osteogenic induction. @entity731 staining was performed on the 14th day after culture; real-time fluorescent quantitative PCR was used to detect the mRNA expressions of @entity2095 , @entity6037 , @entity16835 , @entity21408 , @entity72655 , and @entity60738 on the 21st day after culture; and von Kossa staining was performed on the 28th day after culture. RESULTS: There was no significant difference in the expressions of @entity2095 , @entity12480 , and @entity6667 between the experimental group and control group at 1 and 3 weeks after injection (P > 0.05). There was no significant difference in the percentages of @entity731 positively-stained cells between the experimental group and the control group on the 14th day after culture (P > 0.05). The mRNA expressions of @entity2095 , @entity6037 , @entity16835 , @entity21408 , D1x3, and @entity60738 in osteogenic differentiation-induced BMSCs had also no significant difference between the experimental group and the control group at 1 and 3 weeks after culture (P > 0.05). No significant difference in biomineralization was found between the experimental group and control group at 1 and 3 weeks after culture (P > 0.05). CONCLUSION: Subcutaneous injection of @entity3413 [(5 mg/(kg x d)] for 1 or 3 weeks can affect neither the expressions of bone formation relative factors of proximal tibia trabecular bone nor the osteogenic differentiation of the BMSCs.
