Scanning immuno-electron microscopy of @entity1 XXXX and @entity706 cells: a comparative study of techniques using immunolatex spheres as marker.

@entity1 @entity852 @entity981 @entity2228 @entity706 @entity3584 @entity5399 @entity6494 @entity1778
In this study scanning immuno-electron microscopic (SIEM) techniques were used to identify @entity1 @entity6494 cells. Monodispersed @entity852 (latex) beads were conjugated to specific antisera using @entity3584 , in an attempt to detect surface antigenic components on a variety of cells of known origin. Antisera, mostly immunoglobulin fractions, against @entity1 thymus (T) derived cells, common type @entity981 cells (C/ALL) and surface immunoglobulin (sIg) bearing cells were used to coat latex spheres, while rabbit anti-mouse @entity5399 antiserum or whole human-IgG (gamma-globulin) bound to latex were used as controls in some experiments. The use of SIEM techniques in the direct mode as a simple and sensitive method for labelling surface antigens is described. The disadvantages of the SIEM methodology are also summarized while the requirements for optimal cell preparation using this technique are stressed. The experiments were designed to ascertain whether prolonged fixation of cells could be used prior to incubation of the cells with the marker. In this respect, repeated neutralization of the @entity3584 with @entity2228 is essential. SIEM labelling of cells is random and unreliable without adequate quenching with @entity2228 . The heteroantisera used in this study proved to be adequate and insignificant non-specific attachment and cross reactivity were seen. SIEM adds a further dimension to ultrastructural aspects of immunology and is a potentially useful tool in the study and identification of @entity1778 and @entity706 cells.
