[Contribution of blue-green pigments to hemolytic activity of XXXX cultural fluid].

@entity24206 @entity796 @entity2387 @entity26 @entity1573 @entity224 @entity28907
AIM: To assess the contribution of blue-green pigments of @entity796 to hemolytic activity of its cultural fluid. MATERIALS AND METHODS. Eight hospital strains and reference strain ATCC 15442 were used. Growth dynamics of strains as well as features of accumulation of hemolytic and phospholipase activity were studied. Purified samples of @entity28907 and @entity24206 were extracted by gel-chromatography and chloroform extraction methods. Hemolytic and lecitinase activities of the samples as well as effect of active @entity26 scavengers and chelating agents on these activities were studied. RESULTS: Dynamics of accumulation of hemolytic activity significantly differed from that of phospholipase activity when strains were grown in liquid medium. Chromatographic separation of the pigments from cultural fluid supernatants sharply reduced its hemolytic activity. Purified samples of @entity28907 and @entity24206 were capable to lyse erythrocytes and @entity1573 egg lecitin. These characteristics of the pigments were inhibited by @entity2387 and sensitive to chelating agents. Conclusion. @entity28907 and @entity24206 of pathogenic strains of @entity796 are capable to lyse erythrocytes and suspension of purified @entity1573 egg lecitin, they contribute to total hemolytic activity of pathogenic strains of @entity796 , which is not determined only by phospholipase C produced by microorganism. Lytic activity of the pigments is blocked by @entity2387 and susceptible to some chelating agents. Apparently, this activity is mediated by @entity224 radical and determined by presence of metals with transient valence in pigments' molecules.
