Ultrastructure of XXXX .

@entity1 @entity778 @entity9504 @entity32 @entity267 @entity19 @entity281 @entity2687
PURPOSE: We determined the ultrastructure of @entity19 @entity9504 , a model for @entity1 @entity9504 . METHODS: @entity9504 was induced in C57Bl/6j @entity19 by intrastromal injection of @entity1 adenovirus species D type 37 (HAdV-D37) with a heat-pulled, glass, micropipette needle under compressed air. At select time points after @entity281 , @entity19 were euthanized and their corneas removed, fixed, and sectioned at 70-nm thickness for electron microscopy. RESULTS: Injection of HAdV-D37 into the @entity19 @entity2687 placed virus predominantly in the pericellular corneal stromal matrix. @entity778 was seen bound to and entering stromal cells at 1 and 2 hours after @entity281 , respectively. Cell membrane transit by virus was seen to involve two distinct structures resembling caveolae and macropinosomes. However, later during @entity281 intracellular virus was not seen within membrane-bound organelles. By 8 hours after @entity281 , intracellular virus had accumulated into densely packed, perinuclear arrays. @entity778 disassembly was not obvious at any time point after @entity281 . Infiltrating neutrophils seen by one day after @entity281 had engulfed degraded stromal cells by 4 days after @entity281 . CONCLUSIONS: By transmission electron microscopy, injected HAdV-D37 readily enters stromal cells in the C57Bl/6j @entity19 @entity267 and induces @entity32 , as was shown previously by light microscopy. However, electron microscopy also revealed dense, static arrays of intracytoplasmic virus, suggesting a block in viral capsid disassembly and viral DNA nuclear entry. These findings may explain why @entity1 adenoviruses do not replicate in the @entity19 @entity2687 .
