XXXX Dental Pulp Stem Cells via the NF-kB Pathway.

@entity1 @entity4284 @entity623 @entity27048
BACKGROUND/AIMS: Odontogenic differentiation of @entity1 dental pulp stem cells (HDPSCs) is regulated by multiple factors and signaling molecules. However, their regulatory mechanisms are not completely understood. In this study, we investigated the role of Zinc finger and BTB domain-containing 20 ( @entity27048 ) in odontoblastic differentiation of HDPSCs. METHODS: HDPSCs were obtained from @entity1 third molars and @entity27048 expression was examined by qRT-PCR and western blot. Their osteo/odontogenic differentiation and the involvement of NF-kB pathway were subsequently investigated. RESULTS: The expression of @entity27048 is upregulated in a time-dependent manner during odontogenic differentiation of hDPSCs. Inhibition of @entity27048 reduced osteogenic medium (OM)-induced odontogenic differentiation, reflected in decreased @entity623 ( @entity623 ) activity, mineralized nodule formation and mRNA expression of odonto/osteogenic marker genes. In contrast, overexpression of @entity27048 enhanced @entity623 activity, mineralization and the expression of differentiation marker genes. Furthermore, the expression of @entity4284 was increased by @entity27048 silencing in HDPSCs, whereas @entity27048 overexpression decreased @entity4284 and enhanced nuclear NF-kB p65. Inhibition of the NF-kB pathway significantly suppressed the odontogenic differentiation of HDPSCs induced by @entity27048 . CONCLUSION: This study shows for the first time that @entity27048 plays an important role during odontoblastic differentiation of HDPSCs and may have clinical implications for regenerative endodontics.    2015 S. Karger AG, Basel.
