A microarray based expression profiling of @entity1311 and XXXX resistant MCF-7 cells.

@entity1262 @entity23844 @entity0 @entity986 @entity5 @entity1311 @entity189 @entity1891 @entity7596 @entity4191 @entity72497
Resistance to the broad spectrum of chemotherapeutic agents in @entity5 cell lines and @entity5 has been called multiple drug resistance (MDR). In this study, the molecular mechanisms of resistance to two anticancer agents ( @entity1311 and @entity1891 ) in @entity189 cell line MCF-7 were investigated. Drug resistant sublines to @entity1311 (MCF-7/Pac) and @entity1891 (MCF-7/ @entity23844 ) that were developed from sensitive MCF-7 cells (MCF-7/S) were used. cDNA microarray analysis was performed for the RNA samples of sensitive and resistant cells in duplicate experiments. GeneSpring GX 7.3.1 Software was used in data analysis. The results indicated that the upregulation of @entity1262 gene is the dominating mechanism of the @entity1311 and @entity1891 drug resistance. Additionally the upregulation of the genes encoding the detoxifying enzymes (i.e. @entity4191 ) was observed. Significant downregulation of apoptotic genes (i.e. @entity72497 ) and upregulation of some cell cycle regulatory genes ( @entity986 , @entity7596 etc.) was seen which may be in close relation to MDR in @entity0 . Drug resistant @entity5 cells exhibit different gene expression patterns depending on drug treatment, and each drug resistance phenotype is probably genetically different. Further functional studies are needed to demonstrate the complete set of genes contributing to the drug resistance phenotype in @entity0 cells.
