Presensitization to Ascaris antigens promotes induction of mite-specific IgE upon mite antigen inhalation in XXXX .

@entity1 @entity2199 @entity59 @entity19 @entity566 @entity2329 @entity6527 @entity281 @entity56 @entity1312 @entity6711
BACKGROUND: @entity1 with @entity6711 ( @entity6711 ) @entity566 or @entity2329 produce serum IgE specific to the antigens of @entity6711 or @entity2199 Ascaris, respectively. Although @entity1 IgE cross-reactivity has been reported between @entity6711 and Ascaris antigens, it remains unclear whether it contributes to the pathogenesis of @entity56 . We herein investigated the induction of cross-reactive antibodies and T cells in @entity19 and effects of airway exposure to @entity6711 antigens after preimmunization with Ascaris antigens. METHODS: @entity19 were intraperitoneally immunized with @entity6711 or Ascaris antigens with Alum, followed by the intranasal administration of @entity6711 antigens. Serum antigen-specific IgE and IgG were measured by ELISA. Cytokine release in splenocytes from Ascaris-immunized @entity19 upon in  vitro restimulation with @entity6711 antigens were measured by ELISA. RESULTS: Immunization with Ascaris or @entity6711 antigens induced cross-reactive @entity6527 . Splenocytes from Ascaris-immunized @entity19 released @entity59 and @entity1312 in response to the restimulation with @entity6711 antigens. Subsequent airway exposure to @entity6711 antigens promoted the induction of @entity6711 -specific IgE and upregulation of @entity6711 -specific @entity6527 in Ascaris-immunized @entity19 , whereas these responses were not detected or smaller without the Ascaris presensitization. CONCLUSIONS: We demonstrated that the immunization of na  ve @entity19 with Ascaris antigens induced production of antibodies and differentiation of Th2 cells, which were cross-reactive to @entity6711 antigens, and accelerated induction of serum @entity6711 -specific IgE upon subsequent airway exposure to @entity6711 antigens in @entity19 . These results suggest that sensitization to @entity6711 towards IgE-mediated @entity56 is faster in individuals with a previous history of @entity281 than in those without presensitization to Ascaris.
