The role of LGL/NK cells in surgery-induced promotion of XXXX and its attenuation by @entity1286 .

@entity5 @entity1286 @entity3 @entity35 @entity158 @entity420
Painful stress such as surgery has been shown both to suppress immune function and to promote @entity3 , although the degree to which alterations in immunity underlies the @entity5 -enhancing effects of surgery remains unclear. We recently reported that an experimental laparotomy results in a twofold increase in the number of lung metastases following iv injection of MADB106 @entity5 cells, a natural killer (NK)-sensitive mammary @entity420 cell line, syngeneic to the Fischer 344 @entity35 we studied. Further, the administration of an analgesic dose of @entity1286 prevented these metastatic-enhancing effects of surgery. The aim of the present study was to investigate the role of NK cells in both the metastatic-enhancing effects of surgery and the attenuation of these effects by @entity1286 . Using a simple 2 x 2 experimental design (surgery with anesthesia vs anesthesia only, and @entity1286 vs vehicle), we found that surgery resulted in a decrease in both whole blood NK cytotoxic activity and number of circulating LGL/NK cells assessed 4 h postoperatively. In a second experiment involving an 18-h lung clearance assay, we used the mAb 3.2.3 to deplete @entity35 of LGL/NK cells with the following rationale: if LGL/NK cells are necessary to mediate an event, then in their absence, that event should not occur. Normal and LGL/NK-depleted animals were assigned to the same four experimental groups, and radiolabeled MADB106 @entity5 cells were injected iv 4 h after surgery. In normal animals, there was a significant interaction between surgery and @entity1286 such that @entity1286 attenuated the surgery-induced increase in @entity5 cell retention without affecting @entity5 cell retention in the anesthesia groups. In the LGL/NK-depleted animals, however, although the @entity5 -enhancing effects of surgery remained evident, @entity1286 did not mitigate this outcome. These results suggest that: (a) both LGL/NK cell activity and other factors independent of LGL/NK cells play a role in the surgery-induced increase in @entity5 cell retention; and (b) LGL/NK cells play a critical role in @entity1286 's attenuating effects on this outcome. Finally, these results reinforce concern about the pathogenic consequences of unrelieved @entity158 .
