Transplantation of autologous adipose-derived stem cells ameliorates cardiac function in XXXX with myocardial infarction.

@entity2591 @entity296 @entity7499 @entity95 @entity22524 @entity1391 @entity1188 @entity299
BACKGROUND: Adipose-derived stem cells (ADSCs) are capable of differentiating into cardiomyogenic and endothelial cells in vitro. We tested the hypothesis that transplantation of ADSCs into @entity296 may regenerate infracted myocardium and restore cardiac function. METHODS: ADSCs were isolated from the fatty tissue of New Zealand white @entity95 and cultured in Iscoves modified dulbeccos medium. Three weeks after ligation of left anterior descending coronary artery of @entity95 , either a graft of untreated ADSCs (UASCs, n = 14), @entity2591 -pretreated ADSCs (AASCs, n = 13), or @entity1391 buffer saline (n = 13) were injected into the @entity1188 region. Transmural scar size, cardiac function, and immunohistochemistry were performed 5 weeks after cell transplantation. RESULTS: ADSCs in culture demonstrated a fibroblast-like appearance and expressed CD29, @entity22524 and CD105. Five weeks after cell transplantation, transmural scar size in AASC-implanted hearts was smaller than that of the other hearts. Many ADSCs were differentiated into cardiomyocytes. The AASCs in the prescar appeared more myotube-like. AASCs in the middle of the scar and UASCs, in contrast, were poorly differentiated. Some ADSCs were differentiated into endothelial cells and participate in vessel-like structures formation. All the ADSC-implanted hearts had a greater capillary density in the @entity1188 region than did the control hearts. Statistical analyses revealed significant improvement in left ventricular ejection fraction, myocardial performance index, end-diastolic pressure, and peak +dP/dt, in two groups of ADSC-implanted hearts relative to the control hearts. AASC-implanted hearts had higher peak -dP/dt values than did control, higher ejection fraction and peak +dP/dt values than did UASC-implanted hearts. CONCLUSIONS: ADSCs transplanted into the @entity296 tissue formed @entity299 , induced @entity7499 and improved cardiac function. @entity2591 pretreatment before implantation is desirable for augmenting myogenesis. Transplantation of @entity2591 -treated ADSCs into the @entity296 was more efficient than that of untreated ADSCs in preservation of cardiac function.
