Enhancement of @entity5 initiation and expression of @entity4030 , @entity25785 and XXXX genes in the adenocarcinoma of lung xenograft after vorinostat treatment.

@entity25785 @entity7245 @entity22985 @entity5 @entity19 @entity2 @entity4030 @entity2834
UNASSIGNED: Cancer stem cells (CSCs) are usually tolerant to chemotherapy and radiotherapy and associated with @entity5 relapse. @entity7245 ( @entity7245 ), a histone deacetylase inhibitor (HDACI), is currently being used in clinical trials of @entity2 . However, @entity7245 facilitates the formation of induced pluripotent stem cells from somatic cells. We hypothesized that @entity7245 would mediate the CSCs properties and subsequently confer a more malignant phenotype in @entity2 . Transfected @entity2 cells, which stably expresses a triple fused reporter gene (DsRedm-Fluc-tTKsr39) under the control of @entity2834 promoter was used to establish a xenograft @entity19 model. After the treatment of @entity7245 , H1299 cell line and @entity5 xenografts were sorted by fluorescence-activated cell sorting (FACS) based on aldehyde dehydrogenase (ALDH) activity. We found that @entity7245 could suppress the growth of xenografted @entity5 with decreased proportion of @entity2 cells indicating that @entity7245 may target CSCs. However, @entity7245 significantly enhanced the @entity5 initiating capacity and the expression of malignant genes such as @entity4030 , @entity25785 and @entity22985 in the remaining living ALDH(br) cells. These findings suggested that @entity7245 treatment created a more drug-resistant state in residual ALDH(br) cells. The in vivo imaging technique may facilitate searching and characterization of CSCs.
