Gamma knife irradiation increases cerebral endothelial expression of @entity2071 and XXXX .

@entity1 @entity522 @entity4725 @entity16 @entity4105 @entity880 @entity2071
OBJECTIVE: Alterations in multiple functions of the microvasculature occur in response to gamma irradiation and are thought to contribute to radiation-induced end @entity16 by inducing inflammatory responses, particularly leukocyte infiltration into the affected area. Endothelial cell adhesion molecules (ECAMs) mediate leukocyte adhesion and migration. Here, we validate a method to study the effect of Leksell gamma knife stereotactic radiosurgery on the expression of ECAMs on @entity1 cerebral endothelium at 0, 24, 48, and 72 hours after irradiation. METHODS: A @entity1 brain endothelial cell line (IHEC) was cultured on 12-mm coverslips and subjected to 50 Gy of collimated gamma irradiation with the Leksell gamma knife (Elekta Instruments, Inc., Atlanta, GA). @entity880 dehydrogenase release was measured at 24, 48, and 72 hours after irradiation and @entity522 at 24, 48, 72, 96, and 120 hours. ECAM expression was measured at postirradiation intervals of 0, 24, 48, and 72 hours by cell enzyme-linked immunoabsorbent assay. We used a cell irradiator composed of two chambers. The upper chamber holds the coverslips firmly in place while they are immersed in media. The lower chamber is connected to a peristaltic pump, which pumps water into the chamber and maintains the media in the upper chamber at 37 degrees C through convection. RESULTS: None of the ECAMs tested was significantly elevated compared with the control basally. Twenty-four hours after irradiation, @entity2071 was significantly elevated on brain endothelial cells but there was no significant elevation of @entity4105 . @entity4725 was increased slightly but not significantly and decreased at 48 hours. At 72 hours, @entity4105 expression was significantly increased; @entity2071 and @entity4725 were not altered relative to sham controls. CONCLUSION: Increased ECAM expression and @entity880 dehydrogenase release support the idea that the cerebral microvasculature undergoes an inflammatory response after Leksell gamma knife stereotactic radiosurgery.
