XXXX , Small Cell Variant, Possibly at the Stage of Intracytoplasmic Expression of CD3 in T-Cell Ontogenesis.

@entity1 @entity3329 @entity10911 @entity2902 @entity404 @entity417 @entity706 @entity882 @entity3138 @entity5893 @entity11112 @entity828 @entity6444 @entity3053 @entity272 @entity4587 @entity405 @entity4896
@entity6444 , small cell variant (T-PLL-s), is a rare @entity706 associated with a poor prognosis. We encountered a case of T-PLL-s with a characteristic phenotype. A 67-year-old female was referred to our hospital because of lymphocytosis in August 2013. @entity828 , @entity3329 , and @entity272 were absent. Hematologic examination revealed a white blood cell count of 17.9 * 10(9)/L with 81.2% mature lymphocytes, which were small with a high nuclear/cytoplasmic ratio, lacking a nucleolus and cytoplasmic granules. @entity417 and @entity882 were not observed. Flow cytometric analysis showed that these lymphocytes were positive for @entity3138 , cyCD3, @entity404 , @entity5893 , @entity4587 , @entity10911 , and @entity3053 (partially), but negative for smCD3, smTCR-ab and -y  , cyTCR-b, @entity4896 , @entity405 , @entity2902 , HLA-DR, and terminal deoxynucleotidyl transferase. Polymerase-chain reaction analysis of cells from both the peripheral blood and the bone marrow demonstrated monoclonal rearrangement of TCR-y. A possible rearranged band of the @entity11112 gene was observed by Southern blot analysis. The karyotype of the marrow cells was 46, XX. A diagnosis of T-PLL-s, possibly at the stage of cytoplasmic CD3 expression in the ontogenesis of T-cells, was made. The @entity1 has been asymptomatic, and the white blood cell count has gradually increased during one-year observation, being 69.0 * 10(9)/L with 89.7% lymphocytes in August 2014.
