Induction of glial fibrillary acidic protein immunoreactivity in the XXXX dentate gyrus after adrenalectomy: comparison with neurodegenerative changes using @entity575 impregnation.

@entity575 @entity2819 @entity179 @entity40 @entity35 @entity528
In the present study we performed a light microscopic anatomical comparison of adrenalectomy (ADX)-induced @entity179 using @entity575 impregnation and reaction of astroglial cells using @entity40 immunocytochemistry in the hippocampus of the @entity35 . Three survival times following ADX were studied: 24 hours, 3 days, and 3 weeks. Twenty-four hours following ADX we found no degenerative changes or altered @entity40 immunostaining. Three days after adrenalectomy, argyrophilic somata appeared in the granular cell layer of the dentate gyrus. Argyrophilic dendrites were seen in the molecular layer of the dentate gyrus and neuritic argyrophilia were seen in the mossy fiber layer. Induction of @entity40 immunoreactivity occurred simultaneously with degeneration. Increased @entity40 immunoreactivity could be observed 3 days after adrenalectomy in the molecular layer of the dentate gyrus, granular cell layer, sub and supragranular cell layer, and mossy fiber layer. Size and shape of astroglial cells were changed, and their processes in the molecular layer changed from unidirectional to randomly organized. Degeneration and astroglial reaction were more pronounced 3 weeks after adrenalectomy and both were prevented by adding @entity2819 to the drinking solution. Animals that did not show degenerative changes showed no increased @entity40 immunoreactivity, while both effects were confined to the dentate gyrus and mossy fiber zone. These results show that there is a close relationship between the induction of @entity40 immunoreactivity in the hippocampus of the @entity35 and @entity528 in the dentate gyrus following ADX, both in time and space.
