[Characterization of a XXXX sinensis antigen, calmodulin, and its relationship with @entity1575 ].

@entity3534 @entity32 @entity82 @entity1575 @entity19 @entity14007 @entity409 @entity2441 @entity2440 @entity35 @entity526
OBJECTIVE: To characterize the biological function of calmodulin (CaM) from @entity2440 ( @entity2441 , Cs) and investigate its role in @entity14007 -associated hepatic @entity82 . METHODS: The full-length sequence of CsCaM gene was isolated from Cs cDNA library and its homologues were searched using BLASTx for comparison. Bioinformatics analysis was performed to compare the homologues and predict the physiochemical characteristics and functional domains. The gene was cloned in a prokaryotic plasmid and expressed in @entity526 , and the recombinant protein was purified by affinity chromatography for immunizing @entity35 to produce polyclonal antibodies, whose titer was determined using ELISA analysis. Immunoblotting analysis was carried out to determine of the purity and antibody recognition of CsCaM. Immunofluorescence assay was employed to analyze the tissue location of the protein. A @entity35 model of @entity1575 was established by introperitoneal injection of the recombinant protein. RESULTS: The recombinant CsCaM protein obtained contained 150 @entity3534 with a theoretical molecular mass of 23.4 kD. CsCaM homologue had EF hand motifs. The recombinant pET-30a-CsCaM plasmid expressed in BL21 @entity526 was about 23.4 kD. The total IgG antibody titer in the immunized @entity19 reached the peak level (over 1: 51200) 2 to 4 weeks after the first injection. Immunohistochemistry showed that CsCaM located in the testis of adult @entity2441 . The @entity35 receiving intraperitoneal injection of CsCaM showed severe @entity409 with mild to moderate @entity1575 . CONCLUSION: The pro- @entity32 and pro- @entity82 effects of CsCaM in @entity35 liver suggest its involvement in @entity14007 - associated hepatic @entity82 .
