Targeted delivery of @entity24509 -modified DNA-loaded nanoparticles to XXXX via intravenous administration.

@entity3525 @entity13 @entity970 @entity5 @entity21454 @entity11 @entity2903 @entity21455 @entity24509 @entity968
Gene therapy offers great potential for @entity13 . However, therapeutic genes could not reach @entity11 spontaneously. @entity11 -targeting gene delivery system is highly desired to transfer exogenous genes throughout the @entity5 focus. In this study, the nanoscopic high-branching dendrimer, @entity21454 ( @entity21455 ), was selected as the main vector. @entity24509 ( @entity3525 ), which has been demonstrated to bind specifically to receptor expressed in @entity11 , was exploited as the targeting ligand to conjugate @entity21455 via bifunctional @entity968 ( @entity970 ), yielding @entity21455 - @entity970 - @entity3525 . The cellular uptake of @entity3525 itself was observed apparently in C6 @entity11 cells, almost not in 293 cells. The modification of @entity3525 could significantly increase the cellular uptake of vectors and the DNA-loaded nanoparticles (NPs) in C6 cells. The in vivo distribution of @entity21455 - @entity970 - @entity3525 /DNA NPs in the brain was higher than that of @entity21455 /DNA NPs and @entity21455 - @entity970 /DNA NPs. Furthermore, the gene expression of @entity21455 - @entity970 - @entity3525 /DNA NPs was higher and  broader in @entity11 than that of unmodified and @entity970 -modified counterparts. The TUNEL analysis showed a more wide-extended apoptosis in the @entity3525 -modified group, compared to other groups including commercial @entity2903 group. The median survival time of @entity3525 -modified group and @entity2903 group was 59.5 and 49 days, respectively, significantly longer than that of other groups. The results suggested that @entity3525 could be exploited as a special @entity11 -targeting ligand, and @entity21455 - @entity970 - @entity3525 /DNA NPs is a potential non-viral delivery system for gene therapy of @entity11 via intravenous administration.
