Recombinant lentivirus with enhanced expression of caudal  -related homeobox protein  2 inhibits XXXX @entity14 cell proliferation in  vitro.

@entity1 @entity6628 @entity2803 @entity5 @entity5303 @entity519 @entity98 @entity14 @entity2215
UNASSIGNED: Caudal  -related homeobox protein 2 ( @entity6628 ), a @entity5 suppressor in the adult colon, is overexpressed under a non  - @entity5 specific @entity2803 promoter in certain @entity5 cells; furthermore, non  -specific expression of @entity6628 may result in aberrant side effects in normal cells. The @entity1 @entity2215 ( @entity2215 ) promoter is active in the majority of @entity5 cells but not in normal cells. @entity519 is a key feature of solid @entity5 , and targeted genes may be significantly upregulated by five copies of @entity519  -response elements (HREs) under @entity519 conditions. However, the effect of @entity6628 overexpression, as controlled by five copies of HREs and the @entity2215 promoter, on @entity1 @entity14 ( @entity14 ) cell proliferation in  vitro remains to be fully elucidated. In the current study, a recombinant lentivirus containing the @entity6628 gene under the control of five HREs and the @entity2215 promoter was generated. An immunofluorescence assay was used to detect @entity6628 expression by the 5HhC lentivirus, whereas an @entity98 assay was used to detect the effects of @entity5303 on the viability of LoVo cells. Western blot analysis was conducted in order to determine the relative ratios of recombinant @entity6628 protein to the internal control b-actin, following 5HhC/LoVo  cell culture under normoxic and @entity519 conditions (100, 200, 300, 400  or 500    mol/l  @entity5303 ) for 24  h, then for 12, 24  or 36  h with the optimal concentration (300    mol/l) of @entity5303 . Reverse transcription polymerase chain reaction analysis was used to determine the transcription of recombinant @entity6628 mRNA following culture of 5HhC/LoVo cells under normoxic or @entity519 conditions. Finally, a cloning assay was used to detect the proliferative ability of 5HhC/LoVo and 5Hh cells. High @entity6628 expression was observed in @entity2215  -positive LoVo cells under @entity519 conditions, an effect which was mimicked by treatment with @entity5303 to inhibit LoVo  cell proliferation in  vitro. High expression of @entity6628 therefore provides a promising strategy for the development of novel targeted treatments and gene therapy for @entity14 .
