Relationship between low XXXX receptor values and other prognostic factors in primary breast tumors.

@entity172 @entity1 @entity1420 @entity7273 @entity5 @entity513 @entity6455
BACKGROUND: The current study compared the immunocytochemical expression of @entity172 (ER) and @entity1420 ( @entity7273 ) receptors by malignant breast cells to the hormone receptor concentrations reported from radioligand assays. These values were examined in relation to DNA ploidy and the fraction of cells in S phase. METHODS: ER and @entity7273 concentrations, DNA ploidy, and S-phase fractions were measured by standard techniques with 124 samples of @entity513 . Suspensions of @entity5 cells were examined by immunocytochemical assay (ICA) for the percentages of ER and @entity7273 positive cells. RESULTS: Twenty-six of the 38 @entity5 from @entity1 50 years of age or younger were classified as high S-phase fraction, and 28 @entity5 had aneuploid levels of DNA. The 20 ER positive @entity5 each contained less than 100 fmol/mg. Thirty-nine of the 86 @entity5 from @entity1 older than 50 years were classified as high S phase, and 41 were aneuploid. Sixty-five samples were considered ER positive by radioligand assay. ICA showed that @entity5 in either age group with less than 40 fmol/mg did not contain ER positive cells. The proportion of samples with @entity7273 levels between 10 and 100 fmol/mg was small, and fewer @entity7273 positive @entity5 were categorized as negative when examined by ICA for receptor containing cells. The reclassification of the hormone receptor status of a @entity5 based on @entity6455 appeared to be independent of S-phase and ploidy values. CONCLUSIONS: Tumors that are classified as ER or @entity7273 positive based on accepted cutoff values for radioligand assays may actually be receptor negative because the @entity5 do not appear to contain receptor positive cells.
