Assessment of probiotic potential and anticancer activity of newly isolated vaginal bacterium XXXX 5BL.

@entity1 @entity6741 @entity5 @entity1336
Numerous bacteria in and on its external parts protect the @entity1 body from harmful threats. This study aimed to investigate the potential beneficial effects of the vaginal ecosystem microbiota. A type of bacteria was isolated from vaginal secretions of adolescent and young adult @entity1 , cultured on an appropriate specific culture medium, and then molecularly identified through 16S rDNA gene sequencing. Results of 16S rDNA sequencing revealed that the isolate belongs to the @entity1336 species. The isolated strain exhibited probiotic properties such as low pH and high @entity6741 concentration tolerance, antibiotic susceptibility and antimicrobial activity against some pathogenic bacteria. The anticancer effects of the strain on @entity1 @entity5 cell lines (cervical, HeLa; gastric, AGS; colon, HT-29; breast, MCF-7) and on a @entity1 normal cell line ( @entity1 umbilical vein endothelial cells [HUVEC]) were investigated. Toxic side effects were assessed by studying apoptosis in the treated cells. The strain exhibited desirable probiotic properties and remarkable anticancer activity against the tested @entity1 @entity5 cell lines (P    <=   0.05) with no significant cytotoxic effects on HUVEC normal cells (P    <=   0.05). Overall, the isolated strain showed favorable potential as a bioactive therapeutic agent. Therefore, this strain should be subjected to the other required tests to prove its suitability for clinical therapeutic application.
