XXXX cell proliferation is inhibited by specific modulation frequencies.

@entity1 @entity0 @entity5 @entity65986 @entity71512 @entity157
BACKGROUND: There is clinical evidence that very low and safe levels of amplitude-modulated electromagnetic fields administered via an intrabuccal spoon-shaped probe may elicit therapeutic responses in @entity1 with @entity5 . However, there is no known mechanism explaining the anti-proliferative effect of very low intensity electromagnetic fields. METHODS: To understand the mechanism of this novel approach, @entity157 ( @entity157 ) cells were exposed to 27.12 MHz radiofrequency electromagnetic fields using in vitro exposure systems designed to replicate in vivo conditions. @entity5 cells were exposed to @entity5 -specific modulation frequencies, previously identified by biofeedback methods in @entity1 with a diagnosis of @entity5 . Control modulation frequencies consisted of randomly chosen modulation frequencies within the same 100 Hz-21 kHz range as @entity5 -specific frequencies. RESULTS: The growth of @entity157 and @entity0 cells was significantly decreased by @entity157 -specific and @entity0 -specific modulation frequencies, respectively. However, the same frequencies did not affect proliferation of nonmalignant hepatocytes or breast epithelial cells. Inhibition of @entity157 cell proliferation was associated with downregulation of @entity65986 and @entity71512 . Furthermore, @entity157 -specific modulation frequencies disrupted the mitotic spindle. CONCLUSION: These findings uncover a novel mechanism controlling the growth of @entity5 cells at specific modulation frequencies without affecting normal tissues, which may have broad implications in oncology.
