[The primary study on a novel protein binding to the death domain of the XXXX ].

@entity1 @entity51758 @entity3048 @entity2205
OBJECTIVE: To clone and identify novel proteins binding to the death domain of the @entity3048 ( @entity3048 ). METHODS: The yeast two-hybrid system was used for this study. Automatic sequencing was carried out for DNA sequencing. The sequence homology and the functional domains were analyzed by BLAST and the ScanProsite Tool softwares, respectively. Co-immunoprecipitate method was used to confirm @entity1 @entity51758 ( @entity51758 ) binding specifically with DR4CD (the cytoplasmic domain of @entity3048 ) in HEK293T cells. RESULTS: Two positive clones, named as pADB1 and pADB2, were obtained. BLAST searching showed that the homology of the insert sequence of pADB1 with the mRNA of @entity51758 was 97%. The insert of pADB2 shared no homology with any known peptides in GeneBank. Co-immunoprecipitate analysis further confirmed that @entity51758 could bind to DR4CD in vivo specifically. CONCLUSIONS: @entity51758 may associate with DR4CD in vivo specifically. The functional studies of @entity51758 in signaling pathway mediated by @entity2205 ( @entity2205 ) are in active progress in our laboratory.
