Increased @entity5 XXXX factor alpha production in mesenteric lymph nodes of cirrhotic @entity1 with @entity843 .

@entity1 @entity650 @entity82 @entity39 @entity5 @entity310 @entity843 @entity648 @entity2348 @entity1575 @entity35
BACKGROUND: Cytokines produced in mesenteric lymph nodes of cirrhotic @entity35 with bacterial translocation may participate in circulatory alterations of @entity82 . AIM: To investigate whether cirrhotic @entity1 present an increased local generation of cytokines in mesenteric lymph nodes. METHODS: Mesenteric lymph nodes from 26 cirrhotic and 10 control @entity1 were assessed for @entity5 @entity39 factor alpha ( @entity310 ) and @entity650 mRNA and protein expression by competitive reverse transcription-polymerase chain reaction, and by enzyme immunoassay and immunohistochemistry, respectively. RESULTS: @entity650 levels were not different between cirrhotics and controls. Protein and mRNA @entity310 levels in mesenteric lymph nodes from cirrhotics were higher than in controls (p<0.05). Tissue expression of @entity310 by immunohistochemistry was more abundant in cirrhotics. @entity843 @entity1 showed higher @entity310 levels (47 (34-54) pg/mg protein) than @entity1 without @entity843 (18 (17-25) pg/mg protein) (p<0.001). Elevated @entity310 levels (>28 pg/mg protein) in cirrhotics were associated with a higher @entity1 -Pugh score, the antecedent of @entity843 , a lower prothrombin rate, and higher @entity648 and blood @entity310 levels. The strongest association, confirmed by multivariate analysis, was with the presence of @entity843 (p<0.001). @entity2348 after transplantation, mainly by enteric bacteria, were only detected in @entity1 with high @entity310 levels in mesenteric lymph nodes (33% of @entity1 ; p=0.05). CONCLUSION: @entity1 with advanced @entity1575 , and especially with @entity843 , have increased local production of @entity310 in mesenteric lymph nodes that, in common with experimental @entity82 , may also be induced by bacterial translocation.
