Apoptotic Mechanisms of Peroxisome Proliferator-Activated Receptor-y Activation in Acinar Cells During Acute XXXX .

@entity788 @entity1976 @entity2310 @entity12098 @entity1977 @entity2454 @entity35 @entity8629
OBJECTIVE: The objective of this study was to determine the mechanism by which activation of peroxisome proliferator-activated receptor-y promotes apoptosis of acinar cells in @entity788 . METHODS: AR42j cells pretreated with the peroxisome proliferator-activated receptor-y agonist @entity12098 were activated by @entity8629 as an in vitro model of acute @entity788 . Inflammatory cytokines and amylase were detected by enzyme-linked immunosorbent assay. Cell viability was determined by @entity2454 assay. Cell apoptosis was measured by flow cytometry and terminal deoxynucleotidyl transferase-mediated @entity1977 nick end labeling staining. Activity of caspases was determined. @entity2310 and @entity1976 levels were assayed by Western blot. RESULTS: Cytokines, amylase, and cellular proliferation decreased in @entity12098 -pretreated cells. @entity12098 increased the activity of caspases 3, 8, and 9 in @entity8629 -activated AR42j cells as well as in the pancreas of @entity35 3 hours after induction of severe acute @entity788 . Acinar cell apoptosis was induced by reducing the mitochondrial membrane potential in the @entity12098 group. @entity12098 increased expression of proapoptotic @entity2310 proteins and decreased antiapoptotic @entity1976 in @entity8629 -induced AR42j cells and decreased @entity1976 levels in pancreatic tissue of severe acute @entity788 @entity35 1 and 3 hours after induction. CONCLUSION: @entity12098 may promote apoptosis of acinar cells through both intrinsic and extrinsic apoptotic pathways in acute @entity788 .
