In vitro antimicrobial activity of crude XXXX extracts and fractions of Terminalia catappa and Vitex doniana.

@entity1045 @entity1046 @entity3881 @entity794 @entity869 @entity6251 @entity10439 @entity1068 @entity8701
BACKGROUND: The spread of microorganisms resistant to some antimicrobial agents necessitates the need to search for novel and effective antimicrobial agents. In this study, the antimicrobial activity of Terminalid catappa Linn. (Combretaceae) and Vitex doniana Sweet. (Verbenaceae), two Nigerian medicinal plants used in folk medicines for the management of various ailments related to microbial infections were evaluated. OBJECTIVES: To evaluate the antimicrobial activity of the crude @entity1068 extracts and fractions of the leaves and stem bark of T. catappa and V. doniana. METHODOLOGY: Four crude @entity1068 extracts and 16 ( @entity1045 , @entity1046 , @entity10439 and aqueous) fractions of leaves and stem bark of T. catappa and V doniana were evaluated for in vitro antimicrobial activity against fifteen (15) strains of bacteria and fungi. The antimicrobial activity was determined in a 96-well plate using a resazurin based broth microdilution method. Two standard antimicrobial drugs @entity869 and @entity8701 were included as positive control. RESULTS: The butanoL fraction of stem bark of T. catappa and @entity1068 crude extract of leaf of V don iana displayed the highest antibacterial activity with similar @entity6251 ( @entity6251 ) value of 93.75 microg/mL against @entity794 and @entity3881 . Furthermore, the @entity1046 fraction of stem bark of T. catappa showed the highest antifungal activity with @entity6251 of 187.5 microg/mL against A. sydowi. Amp icillin had @entity6251 of 15.6 and 31.3 microg/mL against @entity794 and B. subtili, respectively while @entity8701 produced @entity6251 of 3.9 microg/mL against A. sydowi. CONCLUSION: Termninalia catappa and Vitex doniana may serve as useful sources of plant derived antimicrobial agents.
