DNA ploidy in XXXX . An image analysis study of 43 cases.

@entity706 @entity5 @entity1074 @entity344 @entity1892 @entity982
OBJECTIVE: To analyze the prognostic importance of DNA ploidy pattern on gastrointestinal (GI) @entity1892 using image cytometry (ICM) and to compare the results with previously published flow cytometry (FCM) data. STUDY DESIGN: Forty-three cases of surgically resected primary @entity1892 were examined. Thirty-eight @entity5 were located in the stomach, 2 in the small intestine, 1 in the large bowel and 2 in both the stomach and small intestine. Six cases were at stage E I 1, 15 at stage E I 2, 20 at stage E II 1 and 1 each at stages III and IV. Histologically, the @entity706 were classified as GI low grade marginal @entity706 of @entity1074 ( @entity1074 ) type (low grade, 12 cases), low grade @entity1074 with a high grade component (mixed type, 10 cases) and GI diffuse large @entity1892 (DLBC) (high grade @entity1074 , 21 cases). After gross removal of nonneoplastic tissue, single cell suspensions were prepared from @entity982 blocks and stained according to Feulgen. Ploidy analysis was done using a custom-made DNA cytometer and Optimas image analysis software (Optimas Corp., Seattle, Washington, U.S.A.). RESULTS: Aneuploidy was found in 42% (5/12 cases) of low grade @entity1074 , 90% (9/10 cases) of mixed @entity706 and 100% (21/21 cases) of @entity344 . DNA ploidy had no significant impact on overall survival time (P = .73). CONCLUSION: ICM analysis showed a higher proportion of @entity344 as compared to that in prior studies using FCM for ploidy determination. Whether DNA ploidy is an independent prognostic factor remains to be determined.
