[107-POS] : Cellular and subcellular localization of angiotensin AT1 and AT2 receptors in XXXX utero-placental units along pregnancy.

@entity1 @entity2178 @entity840 @entity7743
OBJECTIVES: In @entity1 , @entity2178 type 1 and 2 receptors ( @entity7743 and AT2R) have been implicated in trophoblast invasion and placental development. Since the utero-placental unit of @entity840 (GP) and @entity1 share a morphologic structure, hormonal, vasodilator patterns and uterine spiral artery remodeling, this species was deemed suitable to study the spatio-temporal expression of the vasoconstrictive ( @entity7743 ) and vasodilator (AT2R) receptors of @entity2178 . METHODS: Utero-placental units were collected in days (D) 15, 20, 40 and 60 of a 64-67days long pregnancy in Pirbright White @entity840 (n=3 per period). The localization and intensity of expression of @entity7743 and AT2R in different structures of utero-placental units was evaluated by immunohistochemistry. Additionally immunogold electron microscopy for both receptors was performed in day 60. RESULTS: @entity7743 and AT2R were detected along pregnancy in the interlobium, the endothelium and syncytiotrophoblast of the placental labyrinth, subplacenta, giant cells, syncytial streamers, decidua, vascular smooth muscle of spiral arteries and myometrium; in day 20 in perivascular and intramural trophoblasts, while in late pregnancy these were observed in endoluminal trophoblasts in lateral and myometrial spiral arteries. The immunoreactivity of @entity7743 and AT2R was prominent in D15 in syncytial streamers and in D40 in intramural and intraluminal trophoblasts. Immunoelectron microscopy in D60 demonstrated both receptors in cell membrane, cytosol (cyt) and nucleus (nu) in the placenta (Figure). CONCLUSIONS: The spatial pattern of @entity7743 and AT2R supports their association with decidualization, trophoblast invasion, vascular remodeling. This similarity with the @entity1 , added to an analogous intracellular localization of @entity7743 , validates the use of @entity840 to understand the local adaptations of pregnancy. Supported by Fondecyt 1121161 DISCLOSURES: S. Acuna: None. G. Valdes: None.
