[Exogenous XXXX gene expression induces the expression of cardiac markers during P19 cell differentiation in vitro].

@entity7006 @entity39756 @entity14662 @entity23227
OBJECTIVE: To investigate the role of homeobox gene @entity39756 in cardiac myogenesis. METHODS: Two P19 cell lines, namely cells transfected with exogenous expression of @entity39756 gene and non-transfected cells, were cultured in suspension for 4 days to induce cell aggregation, and the cell aggregates were transferred to the Petri dish for further adherent culture. On days 4, 8, 12 and 16 of adherent culture, the expressions of a-sarcomeric actin (a-SA) and @entity23227 ( @entity23227 ) protein were detected by immunocytochemistry, and the mRNA expressions of @entity7006 , a-myosin heavy chain (a-MHC) and @entity14662 ( @entity14662 ) genes by RT-PCR. RESULTS: In the transfected cells, a-SA and @entity23227 protein expressions were detected on days 8, 12 and 16 of adhere culture, and their expressions increased gradually with time. a-SA and @entity23227 expression was significantly higher on day 16 than on day 8 of culture (P<0.01). RT-PCR analysis of the transfected cell showed the presence of @entity7006 expression on day 4 of adherent culture, and the expression increased on days 8 and 12 but decreased on day 16. @entity14662 and a-MHC expressions were found on days 8, 12, and 16, increasing gradually over time and showing significant differences from those on day 4 (P<0.05 or P<0.01). The expression of a-MHC was significantly higher on days 12 and 16 than on day 8 (P<0.05 or P<0.01), and @entity14662 expression was significantly higher on day 16 than on days 8 and 12 (P<0.01). The non-transfected cells were negative for the expressions of all these genes. CONCLUSION: Exogenous expression of @entity39756 gene can induce P19 cells to express cardiac markers in vitro.
