XXXX Content and Enzyme Metabolism in Developing Canola Siliques.

@entity4335 @entity12015 @entity1735 @entity969 @entity1391 @entity505
Little biochemical information is available on @entity1735 metabolism in developing canola ( @entity4335 .) silique (pod) wall and seed tissues. This research examines the @entity1735 contents and @entity969 (Suc) metabolic enzyme activities in different aged silique wall and seed tissues during oil filling. The silique wall partitioned photosynthate into Suc over starch and predominantly accumulated @entity12015 . The silique wall @entity12015 content and soluble acid invertase activity rapidly fell as embryos progressed from the early- to late-cotyledon developmental stages. A similar trend was not evident for alkaline invertase, Suc synthase (SuSy), and Suc- @entity1391 synthase. Silique wall SuSy activities were much higher than source leaves at all times and may serve to supply the substrate for secondary cell wall thickening. In young seeds starch was the predominant accumulated @entity1735 over the sampled developmental range. Seed @entity12015 levels dropped as embryos developed from the early- to midcotyledon stage. @entity12015 and starch were localized to the testa or liquid endosperm, whereas Suc was evenly distributed among seed components. With the switch to oil accumulation, seed SuSy activity increased by 3.6-fold and soluble acid invertase activity decreased by 76%. These data provide valuable baseline knowledge for the genetic manipulation of canola seed @entity505 partitioning.
