Isolation and culture of airway epithelial cells from chronically infected XXXX lungs.

@entity1 @entity137 @entity3273 @entity2890 @entity2379 @entity2892 @entity2176 @entity3403 @entity2177 @entity2889 @entity3400 @entity207 @entity2755 @entity5505 @entity1577 @entity206
We describe procedures for isolating and culturing airway epithelial cells from chronically infected @entity1 lungs. Experience in our laboratory demonstrated the need to balance pathogen eradication against antibiotic toxicity to epithelial cells. To provide a logical basis for antibiotic selection and dose, we systematically analyzed the @entity137 of antibiotics useful against typical pathogens. Alone, @entity3273 , @entity2177 , @entity3403 , and @entity2890 were moderately toxic at concentrations close to those used in cell culture, whereas @entity2379 , @entity5505 , @entity2755 , @entity2892 , @entity2889 , @entity3400 , @entity207 / @entity206 , and @entity2176 were nontoxic even at concentrations many times the antimicrobial level. Epithelial @entity137 of combined antibiotics was additive, with no evidence of competition or synergism. Antibiotics had little effect on initial cell attachment and did not acutely lyse cells, but inhibited subsequent growth. Interestingly, @entity137 decreased markedly with increasing epithelial cell density. @entity1577 ( @entity1577 ) and non- @entity1577 epithelial cells showed no differences in sensitivity to the antibiotics tested and initial exposure to antibiotics did not affect the electrophysiologic properties of resistance or short circuit current in well-differentiated cells. Tailored combinations of antibiotics at appropriate doses killed even multidrug-resistant bacteria. Thus, epithelial cells can usually be cultured from chronically infected @entity1577 airways.
