Tumor XXXX -initiated complement activation stimulates proliferation of @entity2946 cells.

@entity1 @entity2946 @entity54597 @entity39
OBJECTIVE AND DESIGN: We sought to determine the effect of @entity39 -induced activation of the complement protein C3 in @entity2946 . MATERIALS/METHODS: Twelve @entity2946 surgical specimens were evaluated for complement activation using immunohistochemistry, with H_E stains performed on adjacent tissue sections to determine the relationship of complement activation to @entity39 tissue. Flow cytometry and Western blot were performed on three established @entity2946 lines and one surgically-procured cell culture to determine expression of @entity54597 ( @entity54597 ) in @entity2946 . In vitro proliferation of siRNA @entity54597 knockdown cells was compared to that of control siRNA cells with cell line Daoy. RESULTS: Three surgical specimens were found to have @entity39 on H_E sections. In each case, iC3b staining was identified on adjacent sections, limited to the @entity39 region. In no case did @entity39 occur without iC3b staining on adjacent sections. @entity54597 protein was demonstrated on both the three established cell lines and on the surgical culture. Proliferation assays of @entity1 cells with siRNA knockdown vs. control siRNA revealed significantly reduced proliferation at 72  h (p  =  0.001). CONCLUSIONS: @entity39 is associated with complement activation in @entity2946 . @entity2946 cells express @entity54597 , and siRNA-mediated knockdown of @entity54597 inhibits proliferation of these cells in vitro.
