Induced Cortical Neurogenesis after Focal XXXX - Three Active Components from Huang-Lian-Jie-Du Decoction.

@entity1603 @entity64 @entity9096 @entity2806 @entity2500 @entity18174 @entity3088 @entity35 @entity68346 @entity6667 @entity10340
ETHNOPHARMACOLOGICAL RELEVANCE: Huang-Lian-Jie-Du-Decoction (HLJDD) is a Traditional Chinese Medicine (TCM) clinical prescription noted for its neuroprotective effects. The total alkaloids, @entity3088 , and @entity68346 are the main active components of HLJDD. In the present study we explored the possible effects of the total alkaloids, @entity3088 , and @entity68346 from HLJDD on behavioral recovery and cortical neurogenesis after @entity64 . METHODS: The @entity64 model was induced by permanent @entity2500 (pMACO). The total alkaloids (44mg/kg), @entity3088 (50mg/kg), and @entity68346 (80mg/kg) from HLJDD were orally administered for 2 hours after @entity64 and daily thereafter. Neurological function was assessed and then @entity35 were sacrificed 7 days after pMACO. Following repeated intraperitoneal injections of the cell proliferation-specific marker @entity1603 ( @entity1603 ) after @entity64 induction, precursor cell proliferation and differentiation was monitored by immunofluorescent staining. The levels of relevant proteins were determined by western blotting and the mRNA expressions were assessed by quantitative real time-polymerase chain reaction (qRT-PCR). RESULTS: Total alkaloids, @entity3088 and @entity68346 from HLJDD showed improved functional outcome after @entity2806 . The total alkaloids and @entity68346 increased number of @entity1603 -positive cells and enhanced neuronal differentiation in the cortex. Alkaloids-enhanced neurogenesis might be associated with increased @entity6667 , @entity10340 , and @entity18174 protein expression. And the neuroproliferative effect of alkaloids was partially correlated with increased phosphorylation of AKT, and @entity9096 . @entity3088 treatment was found to promote differentiation of cortical precursor cells into neuronal but not glial cells, which may be at least attributable to the regulation of AKT, @entity9096 mRNA and @entity10340 protein levels. CONCLUSIONS: Total alkaloids, @entity68346 and @entity3088 from HLJDD promoted functional recovery likely via enhancing cortical neurogenesis and thus have potential as a treatment for @entity2806 .
