Changes in matrix metalloproteinase network in a spontaneous XXXX model.

@entity1641 @entity73067 @entity37289 @entity1596 @entity1200 @entity44265 @entity73068 @entity37288 @entity792
PURPOSE: @entity792 is a sight-threatening disease in which autoreactive T cells cross the blood-retinal barrier. Molecular mechanisms contributing to the @entity1200 in this @entity792 are not well understood. In this study, the authors investigated the changes in the matrix metalloproteinase network in spontaneous @entity1596 . METHODS: Matrix metalloproteinase (MMP) @entity37288 , @entity37289 , and @entity73067 expression and @entity44265 and @entity73068 ( @entity73068 ) expression were analyzed using Western blot quantification. Enzyme activities were examined with zymography. Expression patterns of network candidates were revealed with immunohistochemistry, comparing physiological appearance and changes in a spontaneous recurrent @entity1596 model. RESULTS: @entity44265 protein expression was found to be decreased in both the vitreous and the retina of a spontaneous model for @entity792 ( @entity1641 recurrent @entity1596 [ERU]), and @entity44265 activity was significantly reduced in ERU vitreous. Functionally associated MMPs such as @entity37288 , @entity73067 , and @entity37289 were found to show altered or shifted expression and activity. Although @entity37288 decreased in ERU vitreous, @entity37289 expression and activity were found to be increased. These changes were reflected by profound changes within uveitic target tissue, where @entity44265 , @entity37289 , and @entity73067 decreased in expression, whereas @entity37288 displayed a shifted expression pattern. @entity73068 , a potential stabilizer of @entity37289 , was found prominently expressed in @entity1641 healthy retina and displayed notable changes in expression patterns accompanied by significant upregulation in autoimmune conditions. Invading cells expressed @entity37289 and @entity73068 . CONCLUSIONS: This study implicates a dysregulation or a change in functional protein-protein interactions in this @entity44265 -associated protein network, together with altered expression of functionally related MMPs.
