Recurrent molecular deletion of the 12p13 region, centromeric to XXXX / @entity9604 , in @entity6444 .

@entity1 @entity9604 @entity1778 @entity6444 @entity13525 @entity1129
INTRODUCTION: @entity6444 is a rare form of @entity1778 which occurs in adults and in younger @entity1 @entity1129 . Among others, complex chromosome aberrations of chromosome 12 have been described in this disease. We searched for deletions of the 12p13 region as the result of these chromosome rearrangements. MATERIAL AND METHODS: Paired leukemic and non-leukemic cells were obtained from a series of 21 @entity1 suffering @entity6444 . Loss of heterozygosity was searched for by microsatellite typing using a fluorescent automated laser DNA sequencer to analyze the amplification products. Proteins were analyzed by Western blot. Southern blot analysis of one @entity1 was conducted. RESULTS AND CONCLUSION: Loss of heterozygosity of the 12p13 region, including the @entity9604 and @entity13525 genes, was detected in nine of these 21 cases (43%). Western and Southern blot analyses of one case demonstrated a biallelic deletion which did not include @entity9604 . Taken together, our results defined a minimal region of deletion of less than one Mb flanked by the markers b312C2T7 and D12S320, excluding @entity9604 as a candidate gene. Deletion of the 12p13 region is thus a highly recurrent genetic event in @entity6444 .
