[Simultaneous determination of glucocorticoids in XXXX plasma by high-pressure liquid chromatography].

@entity821 @entity1045 @entity2819 @entity1068 @entity619 @entity701 @entity1172 @entity291
A method is described for the simultaneous determination of major glucocorticoids in @entity291 plasma, that is, @entity701 , @entity2819 and @entity821 , which includes @entity1172 by high-pressure liquid chromatography and measurement using a UV detector (254 nm). Chromatography on the Zorbax CN column is carried out with the eluent system, that is, @entity1045 / @entity1068 (=87/13 v/v). First, @entity619 was selected as the best internal standard under the previously mentioned conditions. The calibration curves for the glucocorticoids were all clearly linear. The recoveries for each 5 ng of added @entity701 , @entity2819 and @entity821 were 96.7 +/- 3.2%, 100.4 +/- 2.3% and 96.1 +/- 2.2%, respectively. The reproducibility of @entity701 was good and its coefficient of variation of intraassay was small (2.7%), whereas such coefficient was not so in @entity2819 (7.7%) and @entity821 (7.3%). This appears to be due to low concentrations of @entity2819 and @entity821 in the plasma. Specificity of this method to each glucocorticoid was ascertained using plasma from adrenalectomized wethers. In addition, the correlation coefficient between measurements of @entity701 by radioimmunoassay and those by this method was 0.978. In conclusion, the method described in this paper appears to be satisfactory for the simultaneous quantification of plasma glucocorticoids.
