Activation of XXXX in @entity1 endothelial cells increases plasminogen activator inhibitor type-1 expression.

@entity1 @entity11116 @entity3106 @entity2289 @entity3952 @entity5601 @entity7461 @entity1718 @entity4722 @entity6864 @entity11589
OBJECTIVE: To investigate the effect of peroxisome proliferator-activated receptors (PPARs) activators on @entity2289 ( @entity2289 ) expression in @entity1 umbilical vein endothelial cells and elucidate a possible mechanism. METHODS: @entity1 umbilical vein endothelial cells (HUVECs) were obtained from normal fetus, and cultured conventionally. Then the HUVEC were exposed to @entity1718 and @entity11116 in varying concentrations with fresh media. RT-PCR and ELISA were used to determine the expression of @entity5601 and @entity2289 in HUVECs. Transient co-transfection of @entity2289 promoter and @entity5601 gene or @entity6864 gene to ECV304 was performed. RESULTS: @entity5601 , @entity11589 and @entity6864 mRNA in HUVECs were detected by RT-PCR. Treatment of HUVECs with @entity5601 and @entity6864 activators- @entity7461 , @entity4722 , @entity3952 and @entity11116 , but not with @entity3106 could augment PAI-I mRNA expression and protein secretion in a concentration-dependent manner. Proportional induction of @entity2289 promoter activity was observed through increasing amounts of @entity5601 DNA in HUVECs through a transient gene transfection assay, although the mRNA expression of the 3 subtypes of @entity5601 with their activators were not changed compared with controls. CONCLUSIONS: HUVECs express PPARs. PPARs activators may increase @entity2289 expression in endothelial cells (EC). Although PPARs expression was not enhanced after being stimulated by their activators in EC, the functionally active @entity5601 is probably involved in regulating @entity2289 expression in EC.
