[Study on the morphological damage and cell apoptosis of nerve tissue in @entity19 exposed to XXXX ].

@entity1 @entity4485 @entity23929 @entity39 @entity19 @entity3014 @entity1977 @entity3015 @entity1604 @entity2359
OBJECTIVE: To study the toxicological effects of @entity3014 ( @entity3015 ) on @entity1 animal's nerve tissue. METHODS: 50 Kunming @entity19 were divided into 5 groups at random, the exposed groups(3 dose level groups), the vehicle control group and standard control group. Every group got 10 @entity19 . The exposed groups were treated by intraperitoneal injection with @entity3015 dissolved in vegetable oil at 7.8, 3.2 and 1.3 mg/kg respectively, 4 times/week, for 10 weeks, the vehicle control group were given vegetable oil and the standard control group were not given any treatment. All the @entity19 were anesthetized with 0.02 mol/L @entity4485 and infused with 1.33 mol/L @entity1604 dissolved in PBS through heart after 10 weeks. Then the brain, spinal cord and sciatic nerve were removed. Slices of these tissues were made and morphological changes were observed by optical microscope and electron-microscope. Cell appoptosis was examined by TUNEL( @entity23929 -mediated x- @entity1977 nick end labeling) method. RESULTS: Morphological observations showed tissue injury in @entity3015 exposed groups. There were focal @entity39 areas found in the high-dose group. The cell apoptosis rates in 3.2 and 1.3 mg/kg groups were 90.02%-94.22% and 62.45%-77.54% respectively, significantly higher than those of vehicle control group and standard control group(4.60%-5.57%). CONCLUSION: @entity3015 is @entity2359 . It could damage the nerve tissue as well as induce DNA breaks and cell apoptosis.
