Contribution of the @entity33507 receptor-mediated pathway to XXXX in @entity1007 .

@entity1748 @entity2871 @entity753 @entity1007 @entity1423 @entity33507 @entity19 @entity37502 @entity13436 @entity21784 @entity3352 @entity857 @entity65 @entity165
BACKGROUND: In @entity1007 , platelets demonstrate increased reactivity and promote the development of @entity65 . OBJECTIVE: This study was carried out to investigate the contribution of the @entity857 receptor @entity33507 -mediated pathway to @entity1423 due to @entity1007 . METHODS: @entity1748 -deficient @entity19 and C57Bl/6 wild-type @entity19 were fed on normal chow and high-fat (Western or Paigen) diets for 8 weeks to generate differently elevated @entity165 levels. @entity33507 receptor-induced functional responses via G(i) signaling were studied ex vivo when washed @entity19 platelets were activated by 2MeSADP and @entity21784 agonist AYPGKF in the presence and absence of @entity3352 . Platelet aggregation and secretion, a(IIb)b(3) receptor activation and the phosphorylation of @entity13436 ( @entity13436 ) and Akt were analyzed. RESULTS: Plasma @entity165 levels ranged from 69    10 to 1011    185 mg dL(-1) depending on diet in @entity19 with different genotypes. Agonist-dependent aggregation, dense and a-granule secretion and JON/A binding were gradually and significantly (P < 0.05) augmented at low agonist concentration in correlation with the increasing plasma @entity165 levels, even if elevated @entity2871 generation was blocked. These functional responses were induced via increased levels of G(i) -mediated @entity13436 and Akt phosphorylation in @entity753 @entity19 vs. normocholesterolemic animals. In addition, blocking of the @entity33507 receptor by @entity37502 (Cangrelor) resulted in strongly reduced @entity1423 in @entity19 with elevated @entity165 levels compared with normocholesterolemic controls. CONCLUSIONS: These data revealed that the @entity33507 receptor pathway was substantially involved in @entity1423 associated with mild and severe @entity1007 .
