[Prevention of XXXX by combination of @entity4447 and perfect capsule].

@entity1 @entity4447 @entity231 @entity9188 @entity9058 @entity593 @entity460 @entity3236
OBJECTIVE: Despite recent improvements in IOL design, @entity3236 ( @entity3236 ) remains a significant clinical problem after @entity231 surgery. The Perfect Capsule device (Milvella, Ltd., Epping, Australia) permits the introduction and subsequent removal of potentially toxic agents into the closed capsular bag. The present purpose was to detect the effectiveness of @entity4447 ( @entity9188 ) to cultured @entity1 lens cells and cells within the @entity1 capsular bag. METHODS: All experiments were carried out with 2 minutes exposure to @entity9188 . Effect of @entity9188 on FHL124 cells growth was tested by MTT. Changes in cell @entity460 levels induced by high concentration of @entity9188 were measured by real-time fluorometric single-cell digital imaging techniques after @entity593 incorporation. In vitro @entity1 capsular bags were also tested after a sham surgery was performed using the Perfect Capsule device to form a closed system. @entity9188 (10, 30, 100, 300, 1000   mol/L) were used with donor match-pairs treated with medium alone.(n = 3 in all cases).On-going observations were by phase-contrast microscopy. Cellular architecture was examined by fluorescence cytochemistry. RESULTS: @entity9188 of 100   mol/L and above inhibited the growth of @entity9058 124 cells in a dose-dependent manner with 2 minutes exposure (t = 5.217, P < 0.01, IC(50) is 130   mol/L) @entity9188 depleted the @entity460 store and consequently lead to a loss of @entity460 signaling.Moreover, 1   10(4)   mol/L @entity9188 had a moderate effect on the @entity460 influx pathway, inhibition on the amplitude and rapidity is (43.24    2.98)% (t = 3.134, P < 0.01) and (46.27    6.01)% (t = 3.521, P < 0.01), respectively. No cell left in the vitro @entity1 capsular bags after being treated with 1   10(4)   mol/L @entity9188 . CONCLUSIONS: @entity9188 can eliminate the @entity1 lens epithelium cells remained in the capsular bag in @entity231 surgery, which may help to prevent @entity3236 . The application of @entity9188 to cells within the capsular bag for a 2 minute window using the Perfect Capsule system predicts this compound will have therapeutic benefit to @entity1 in vivo.
