Redistribution of ovarian blood flow after injection of XXXX chorionic gonadotropin and luteinizing hormone in the adult pseudopregnant @entity35 .

@entity1 @entity43 @entity1420 @entity1465 @entity1920 @entity2670 @entity35
It is well known that LH and @entity1 @entity1465 ( @entity1465 ) induce an @entity2670 . The effect of LH/ @entity1465 on luteal blood flow, however, is unknown. This work studies the effect of @entity1465 on both @entity43 at different stages of pseudopregnancy in adult female @entity35 . Pseudopregnancy was induced by mating with sterile male @entity35 . The length of pseudopregnancy was 13 +/- 1 days and, during this time, blood flow was measured by the injection of radioactive microspheres during anesthesia. At autopsy, the corpora lutea were identified and extirpated under a stereomicroscope. These, and the remaining ovary, were then counted for radioactivity and the blood flow was calculated. @entity1420 levels were determined in plasma and ovarian tissues. Furthermore, the responsiveness of @entity1920 cyclase was tested in ovarian tissues at day 6 of pseudopregnancy. An intraarterial injection of @entity1465 (50 IU) or vehicle (saline) was given 20 min before the blood flow determinations in anesthetized @entity35 . The luteal blood flow was not changed by @entity1465 on days 2, 6, and 11 of pseudopregnancy, whereas in the remaining ovary the blood flow increased more than 2-fold, thereby resulting in redistribution of the blood flow. Ten micrograms of NIH-LH-B9, tested at day 6 of pseudopregnancy, mimicked the effect of @entity1465 . At day 6 of pseudopregnancy, @entity1465 (50 IU) was given ip to conscious @entity35 200 min and 24 h before blood flow determinations. At 200 min after @entity1465 there was a more pronounced redistribution of ovarian blood flow with a 45% reduction in luteal blood flow and a 4-fold increase in flow through the remaining ovary. LH as well as @entity1465 doubled the @entity1420 content of the remaining ovary. In the corpora lutea an increased @entity1420 content was seen after 200 min of @entity1465 exposure. At 24 h after @entity1465 injection, all parameters had returned to control levels except that @entity1920 cyclase was nonresponsive. The increase in the total ovarian blood flow coincides with the increased steroidogenesis and these effects are likely due to release of metabolites and/or vasoactive substances. Despite this increase, the blood flow of the corpus luteum was not increased rendering vascular mechanisms unlikely as a part of the acute LH/ @entity1465 effects on corpus luteum of pseudopregnancy.
