Involvement of autophagy inhibition in Brucea javanica oil emulsion-induced XXXX cell @entity204 .

@entity1 @entity7970 @entity2667 @entity5 @entity4404 @entity694 @entity2822 @entity21684 @entity204
UNASSIGNED: Brucea javanica oil emulsion (BJOE), the petroleum ether extract of B. javanica emulsified by phospholipid, is widely used in China as an anticancer agent. The extracts from B. javanica induce @entity5 cell @entity204 by various mechanisms; however, it is not known whether these mechanisms involve autophagy, which is an important process in @entity5 development and treatment. Thus, the current study aimed to investigate whether BJOE modulates autophagy in HCT116 @entity1 @entity694 cells and whether modulation of autophagy is an anticancer mechanism of BJOE. Immunoblotting was employed to analyze the protein expression levels of microtubule-associated protein light-chain 3 ( @entity21684 ), a specific protein marker of autophagy, in HCT116 @entity5 cells following exposure to BJOE. The apoptosis rate of the HCT116 @entity5 cells was detected by performing an @entity4404 - @entity2822 / @entity2667 assay. According to the effect of BJOE administration on autophagy in the HCT116 @entity5 cells (induction or suppression), a functionally opposite agent (autophagy suppressor or inducer) was applied to counteract this effect, and the apoptosis rate of the @entity5 cells was detected again. The role of autophagy (pro-survival or pro- @entity204 ) was demonstrated by comparing the rates of apoptotic @entity5 cells prior to and following the counteraction. The results revealed that BJOE suppressed the protein expression levels of @entity21684 , including the @entity21684 -I and @entity21684 -II forms, and induced apoptosis in the HCT116 @entity5 cells with a high level of basal @entity21684 . The apoptosis-inducing activity of BJOE was significantly attenuated when autophagy was induced by the administration of @entity7970 , an autophagy inducer. The data indicates that autophagy inhibition is involved in BJOE-induced @entity5 cell @entity204 , and that this inhibition may be a potential anticancer mechanism of BJOE.
