@entity4447 induces apoptosis equally in @entity1619 MOLT-4 cells and XXXX -expressing @entity5699 -resistant MOLT-4 cells.

@entity1 @entity4447 @entity1619 @entity9188 @entity5699 @entity3514 @entity2667 @entity98 @entity2823 @entity7895 @entity10876
PURPOSE: To investigate the effects of @entity4447 ( @entity9188 ) on @entity1 @entity1619 MOLT-4 cells and @entity7895 -expressing @entity5699 -resistant MOLT-4 (MOLT-4/DNR) cells. METHODS: Cell growth was measured by an @entity98 assay. Cell viability was determined by a dye exclusion test. The level of @entity7895 expression was estimated using @entity10876 -conjugated anti- @entity7895 monoclonal antibody 17F9. The function of @entity7895 was evaluated in terms of @entity3514 ( @entity3514 ) efflux. The percentage of cells undergoing apoptosis was determined by flow cytometry after staining with annexin V- @entity2823 and @entity2667 . RESULTS: @entity9188 inhibited the growth and survival of MOLT-4 and MOLT-4/DNR cells in a time- and dose-dependent manner. The 50% inhibitory concentrations of @entity9188 (IC(50)) against the growth of these cell lines were 5.1 micromol/l and 5.0 micromol/l, respectively, when the cells were treated with @entity9188 for 96 h. @entity9188 induced an apoptotic morphology in both MOLT-4 and MOLT-4/DNR cell lines. These effects of @entity9188 were time- and dose-dependent when the two cell lines were incubated in the presence of 1-8 micromol/l of @entity9188 for 3-144 h. @entity9188 treatment for 3 to 24 h at 5.0 micromol/l did not change the percentage of @entity7895 -expressing cells or the efflux ability of MOLT-4/DNR cells. CONCLUSION: @entity9188 inhibited growth and induced apoptosis equally in MOLT-4 and MOLT-4/DNR cells, and this suppressive effect did not influence @entity7895 expression or function in MOLT-4/DNR cells.
