Cytoplasmic signalling pathways in alveolar macrophages involved in the production of @entity842 after stimulation with excretory/secretory antigens of XXXX .

@entity13557 @entity6566 @entity1855 @entity5603 @entity37239 @entity1426 @entity3543 @entity842 @entity460 @entity9213
We studied the cytoplasmic signalling pathways involved in the generation of @entity842 (NO) after stimulation with adult excretory/secretory antigens (ESA) of @entity5603 . The pathways of phospholipase A2 (PLA2) and phospholipase C (PLC) were considered as potentially involved in the synthesis of @entity842 . We used inhibitors of these pathways at different levels. Several concentrations of @entity13557 , @entity1855 , @entity37239 and staurosporine were used to inhibit the PLC pathway. Inhibition of the PLA2 pathway was attempted with mepacrine, @entity6566 or @entity9213 . @entity13557 , @entity1855 and @entity37239 reduced the production of NO induced by ESA in a concentration-dependent manner. Regarding the PLA2 pathway, a range of concentrations of @entity3543 greatly reduced the production of NO induced by ESA. Meloxicam inhibition was always higher than 50%. @entity6566 showed a dose-dependent effect on the production of NO induced by the ESA. Our results suggest that both the PLC and the PLA2 pathways play an essential role in activating the production of macrophage NO triggered by the ESA of @entity5603 . This could indicate that NO production in our experimental conditions is due to both an increase of intracellular @entity460 and to the participation of the @entity1426 cascade. The implications of these activations on the host-parasite relationship are discussed and compared with LPS-stimulated macrophages.
