Extracorporeal shock wave therapy ameliorates cyclophosphamide-induced @entity35 @entity2335 though inhibiting XXXX and oxidative stress-in vitro and in vivo experiment studies.

@entity4665 @entity32 @entity16620 @entity1506 @entity568 @entity19984 @entity26832 @entity1505 @entity12613 @entity8562 @entity4026 @entity6929 @entity22519 @entity35 @entity811 @entity2335 @entity23346
BACKGROUND: We investigated whether extracorporeal shock wave (ECSW) therapy can attenuate cyclophosphamide (CYP)-induced @entity2335 ( @entity2335 ) in @entity35 . METHODS AND RESULTS: Eighteen male-adult @entity35 were equally divided into group 1 (sham control), group 2 ( @entity2335 induced by 150 mg/kg CYP by intra-peritoneal injection) and group 3 ( @entity2335 + ECSW 200 impulses at 0.11 mJ/mm(2) to the urinary bladder at 3 and 24 h after CYP treatment). Smooth-muscle cells co-culture with menadione (25   M) with and without ECSW treatment was performed. Western-blot results demonstrated that ECSW significant attenuated oxidative stress and inflammatory reactions in this in-vitro studies (all p < 0.001). 24-hour urine amount and microscopic findings of red-blood-cell count (i.e., @entity811 ) were higher in group 2 than in groups 1 and 3, and significantly higher in group 3 than in group 1 (all p < 0.001). The urine levels of albumin and @entity568 showed an identical pattern of @entity811 among all three groups (all p < 0.001). The cellular and mRNA expressions of @entity23346 ( @entity19984 )+, @entity26832 +, @entity22519 +, substance p+, and @entity4665 + cells in the bladder tissue exhibited an identical pattern of @entity811 among all groups (all p < 0.0001). The integrity of epithelial layer and collagen-deposition area as stained by Sirius red displayed an opposite pattern of @entity811 among the three groups (p < 0.0001). The protein expression of IL-12, @entity1506 , @entity1505 , @entity4026 , @entity6929 , @entity12613 , @entity8562 , @entity16620 , and Oxyblot displayed an identical pattern of @entity811 among all groups (all p < 0.01). CONCLUSION: ECSW therapy markedly attenuated CYP-induced @entity2335 through inhibitions of the @entity32 and oxidative stress.
