[Heterologous expression of attacin gene from Musca domestica in XXXX ].

@entity11185 @entity10101 @entity10056 @entity1042 @entity2624 @entity214
OBJECTIVE: To construct recombinant expression plasmid with an antibacterial peptide (attacin) mature peptide gene from Musca domestica and express an antibacterial peptide attacin in @entity10056 . METHODS: The coding region of attacin mature peptide was cloned into the expression vector pPIC9K. The recombinant plasmid was linearized by digestion with Sac I and transformed into @entity10101 by electroporation. The insert clones containing multiple copies were selected with @entity11185 , pha, enotype and PCR. The expression of @entity10101 /pPIC9K-attacin were induced with @entity1042 . The supernatant of the expressed protein was analyzed by @entity214 -PAGE and Western blotting. The anti-bacterial activity of expression product was analyzed by agarose diffusion assay. RESULTS: The expression plasmid was constructed and transformed into @entity10101 . @entity214 -PAGE and Western blotting analysis indicated that the recombinant containing recombinant plasmid pPIC9K-attacin expressed a Mr 22000 protein. The expression product inhibited the growth of @entity2624 D31. CONCLUSION: The attacin gene from Musca domestica has been expressed in @entity10056 .
