Insulin-like growth factor binding proteins in the XXXX adrenal gland.

@entity1 @entity12561 @entity2301 @entity10116 @entity10513 @entity442 @entity49001 @entity865 @entity21419 @entity864
Insulin-like growth factors (IGFs) are thought to be important regulators of adrenocortical growth and steroidogenesis. IGFs are usually complexed with a family of specific IGF-binding proteins (IGFBPs) in serum, other body fluids, and in conditioned media of a variety of cell types. IGFBPs may either inhibit or potentiate the effects of IGFs. In the present study we have investigated the gene expression of the IGFBPs and IGF receptors in @entity1 fetal ( @entity21419 ) and adult (HAA) adrenals. Northern blotting and/or reverse transcription polymerase chain reaction (RT-PCR) methods were used. IGFBP secretion into the cell culture medium was studied in primary cell cultures by Western ligand blotting and by radioimmunoassays. @entity10116 mRNA expression was low in adrenals: Northern blots were negative, but RT-PCR revealed @entity10116 mRNA in @entity21419 . @entity10513 mRNA was equally expressed in both @entity21419 and HAA with no differences in signal intensities by Northern blotting. @entity2301 mRNA was detected in @entity21419 but not in HAA by Northern blotting. @entity12561 mRNA was expressed equally in both @entity21419 and HAA. @entity49001 mRNA expression was more abundant in HAA than in @entity21419 . @entity442 and type I and type II IGF receptor mRNAs were equally expressed in both @entity21419 and HAA. @entity864 ( @entity865 ), a protein kinase regulator, upregulated @entity10116 in @entity21419 cultures as determined by RIA, but ACTH was without effect. @entity10513 was not regulated by @entity865 or ACTH neither at protein nor at mRNA level. @entity2301 was downregulated by @entity865 both at protein and mRNA levels, but it was not affected by ACTH.(ABSTRACT TRUNCATED AT 250 WORDS)
