XXXX activates @entity16091 in @entity1 airway epithelial cells via a G protein-coupled receptor.

@entity1 @entity268 @entity844 @entity4990 @entity3952 @entity2366 @entity8435 @entity4993 @entity8558 @entity15266 @entity6975 @entity460 @entity16091 @entity32361 @entity15267 @entity62126 @entity1928 @entity5595
@entity5595 (SPP) acts as a first messenger in immortalized @entity1 airway epithelial cells (CFNPE9o(-)), possibly interacting with an Edg family receptor. Expression of the SPP receptors @entity6975 and @entity15267 , as well as a low level of @entity15266 /H218, was detected in these cells, in agreement with their ability to specifically bind SPP. The related lipids, @entity4990 and @entity4993 , were unable to displace SPP from its high affinity binding sites, suggesting that the biological responses to these different lysolipids are mediated by distinct receptors. SPP markedly inhibited @entity1928 -stimulated @entity268 accumulation in a dose-dependent manner and caused a remarkable elevation of intracellular @entity460 , both effects being sensitive to pertussis toxin treatment. Most importantly, SPP stimulated @entity8558 formation, which was maximal after 2 min and decreased within 8-10 min. In the presence of @entity32361 , suppression of SPP-induced @entity8558 formation and production of @entity62126 were found, clearly indicating activation of @entity16091 ( @entity16091 ). This finding was also confirmed by analysis of the @entity844 composition of @entity8558 , showing an increase in the monounsaturated @entity3952 only. The decrease of @entity8558 level after 8-10 min incubation with SPP was accompanied by a parallel increase of @entity2366 production, which was abolished in the presence of @entity32361 . This result indicates that activation of @entity16091 is followed by stimulation of @entity8435 phosphohydrolase activity. @entity8558 formation was insensitive to protein kinase C inhibitors and almost completely inhibited by pertussis toxin treatment, suggesting that SPP activates @entity16091 via a G(i/o) protein-coupled receptor.
