[Resistance of multicellular spheroids to taxol in @entity1 XXXX and its mechanism].

@entity1 @entity43 @entity1262 @entity5 @entity3460
BACKGROUND _ OBJECTIVE: Cytotoxic anticancer drugs are less effective in killing @entity5 cells grown as multicellular spheroids than monolayer cell cultures. The aim of this study was to investigate the molecular mechanism of chemoresistance. METHODS: @entity43 A2780, CAOV3 multicellular spheroids were obtained from three-dimensional culture. Expression of @entity1262 ( @entity1262 ) was determined using Western blot analysis and flow cytometry (FCM). The subcellular distribution of @entity1262 was also determined using laser confocal microscope. Reverse transcription-polymerase chain reaction (RT-PCR) was employed to detect the @entity1262 mRNA of both monolayer cells and multicellular spheroids. Cell cycle profiles and apoptosis were also analyzed using FACS. The resistance was detected with @entity3460 exclusion testing. RESULTS: Compared with control cells, no expression of @entity1262 was detected in monolayer cells, but expression of @entity1262 in aggregate cells was significantly elevated(P< 0.05). The @entity1262 mRNA positive nodes were confirmed by RT-PCR in the aggregate cells. The percentage of cells increased in G(0)-G(1) phase and decreased in S and G(2)-M phase significantly in spheroids cells. Spheroids cells showed higher cell viability than monolayer cells (P(A2780)=0.003, P( @entity1 )=0.015). More apoptotic cells were induced by Taxol in MCS cells than in monolayer cells. CONCLUSION: @entity43 A2780, CAOV3 multicellular spheroids cultures induced cell resistance to Taxol. High expression of @entity1262 was induced in ovarian multicellular spheroids and the cells were arrested in G(0)-G(1) phase.
