Reparative properties of the traditional Chinese medicine Cordyceps sinensis (Chinese caterpillar mushroom) using HT29 cell culture and @entity35 XXXX .

@entity1 @entity1819 @entity415 @entity35 @entity3352 @entity694 @entity1068 @entity130
Cordyceps sinensis (CS) is a traditional Chinese medicine and health food used to support many organ systems. It is commercially produced by cultivation in a liquid medium or on a solid (grain/ @entity415 ) phase. We tested the effects of hot water extracts of liquid-phase and solid-phase commercially grown CS on its ability to influence proliferation (using Alamar blue, an oxidation/reduction indicator), migration (serial-wounded monolayer photomicroscopy), invasion through collagen gel (fluorometric assay) and @entity3352 -induced apoptosis (active caspase-3 colorimetric assay) of @entity1 @entity694 HT29 cells. An in vivo study used a @entity35 @entity1819 model ( @entity3352 20 mg/kg and 4 h restraint with oral administration). The CS extract stimulated cell proliferation threefold when added at 10  g/ml (P < 0  01). Cell migration increased by 69 % and invasion by 17 % when CS was added at 5 mg/ml (P < 0  01). The results also showed that 93 % of the pro-proliferative activity was soluble in @entity1068 , whereas pro-migratory activity was divided (61:49) into both @entity1068 -soluble and @entity1068 -insoluble sub-fractions. @entity3352 -induced apoptosis was not affected by the presence of CS. CS reduced the amount of @entity1819 by 63 % when administered orally at 20 mg/ml (P < 0  01), the results being similar to using the potent cytoprotective agent epidermal growth factor at 25  g/ml (83 % reduction). We conclude that both methods of cultivated CS possess biological activity when analysed using a variety of gut models of @entity130 . Functional foods, such as CS, could provide a novel approach for the prevention and treatment of @entity130 .
