Functional Effects of a Tissue-Engineered Cardiac Patch From XXXX Induced Pluripotent Stem Cell-Derived Cardiomyocytes in a @entity35 Infarct Model.

@entity1 @entity583 @entity1188 @entity35
UNASSIGNED: : A tissue-engineered cardiac patch provides a method to deliver cardiomyoctes to the injured myocardium with high cell retention and large, controlled @entity1188 coverage, enhancing the ability of cells to limit remodeling after @entity1188 . The patch environment can also yield increased survival. In the present study, we sought to assess the efficacy of a cardiac patch made from @entity1 induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to engraft and limit left ventricular (LV) remodeling acutely after @entity1188 . Cardiac patches were created from hiPSC-CMs and @entity1 pericytes (PCs) entrapped in a fibrin gel and implanted acutely onto athymic @entity35 hearts. hiPSC-CMs not only remained viable after in vivo culture, but also increased in number by as much as twofold, consistent with colocalization of human nuclear antigen, cardiac troponin T, and Ki-67 staining. CM+PC patches led to reduced @entity1188 sizes compared with @entity583 -only controls at week 4, and CM+PC patch recipient hearts exhibited greater fractional shortening over all groups at both 1 and 4 weeks after transplantation. However, a decline occurred in fractional shortening for all groups over 4 weeks, and LV thinning was not mitigated. CM+PC patches became vascularized in vivo, and microvessels were more abundant in the host myocardium border zone, suggesting a paracrine mechanism for the improved cardiac function. PCs in a PC-only control patch did not survive 4 weeks in vivo. Our results indicate that cardiac patches containing hiPSC-CMs engraft onto acute @entity1188 , and the hiPSC-CMs survive, proliferate, and contribute to a reduction in @entity1188 size and improvements in cardiac function. SIGNIFICANCE: In the present study, a cardiac patch was created from @entity1 induced pluripotent stem cell-derived cardiomyocytes and @entity1 pericytes entrapped in a fibrin gel, and it was transplanted onto infarcted @entity35 myocardium. It was found that a patch that contained both cardiomyocytes and pericytes survived transplantation and resulted in improved cardiac function and a reduced @entity1188 size compared with controls.
