Amplification and overexpression of the @entity5100 regulator @entity44810 in XXXX .

@entity5 @entity44810 @entity5100 @entity1017 @entity4177
There is evidence that 8q amplification is associated with poor prognosis in @entity4177 . A previous comparative genomic hybridization analysis identified a critical region in chromosomal bands 8q11.2-q13. Using restriction landmark genomic scanning in combination with a virtual genome scan, we showed that this region is delineated by sequences within contig NT_008183 of chromosomal subbands 8q11.22-q11.23. A real-time PCR-based genomic copy number assay of 20 @entity4177 revealed @entity1017 in this critical chromosomal region in eight @entity5 . The expression of four genes and expressed sequence tags (ESTs) within this newly defined region was assayed by real-time reverse transcriptase polymerase chain reaction (RT-PCR) in four @entity5 with and six @entity5 without @entity1017 . The @entity44810 oncogene was found to be highly expressed in all but one @entity5 compared to normal liver tissue. Furthermore, quantitative RT-PCR revealed that the expression level of the developmentally regulated transcription factor @entity44810 was 3-12 times greater in @entity5 and cell lines compared to age-matched normal liver and comparable to the expression in fetal liver tissue. @entity44810 has been shown be a transcriptional activator of @entity5100 in other @entity5 types. Using luciferase reporter assays, we demonstrated that @entity44810 transactivates transcription from the embryonic @entity5100 promoter P3, also in @entity4177 cell lines. Thus, our results provide evidence that @entity44810 overexpression may be responsible for the frequently observed up-regulation of @entity5100 in @entity4177 and therefore may be implicated in the molecular pathogenesis of this childhood @entity5 .
