Immunocytochemical localization of immunoglobulins at the corneal surface of the XXXX .

@entity19 @entity40261 @entity9686 @entity9749 @entity72415
Immunoglobulins A, G1, @entity72415 and M were localized at the corneal surface of adult Swiss-Webster @entity19 using Sternberger's peroxidase anti-peroxidase (PAP) technique. Immunoglobulins G2b, G3 and E and lysozyme were not present at the corneal surface of the @entity19 . Light microscopy of whole @entity19 eyes incubated for the detection of @entity40261 ( @entity40261 ) demonstrated a population of positively-stained corneal surface cells. @entity9686 , @entity9749 and IgM could not be similarly detected at the light microscopic level. At the transmission electron microscopic level, a greater amount of staining for @entity40261 was localized over the mature corneal surface cells. A lesser amount of @entity40261 staining occurred over the young and mature corneal surface cells. In contrast to this, @entity9686 , @entity9749 and IgM were randomly distributed over the corneal surface and were not preferentially localized over young or mature surface cells. The staining for @entity9686 , @entity9749 and IgM was less than the staining for @entity40261 at the corneal surface. @entity40261 was also localized at the corneal surface using an indirect immunoferritin technique. These studies suggest that immunoglobulins A, G and M are intimately associated with the glycocalyx and microvilli of the corneal surface cells. At present it is not known whether these immunoglobulins are bound directly to the surface cell apical plasma membrane or indirectly via mucus glycoproteins at the ocular surface.
