DNA methyltransferase of XXXX Marburg: purification, properties and further evidence of specificity.

@entity7682 @entity1025 @entity3208 @entity10043 @entity3281 @entity1133 @entity5538 @entity3881 @entity7391 @entity526 @entity11667
@entity3881 Marburg strain displays DNA methyltransferase activity. This enzyme, M.BsuM, methylates @entity5538 in the sequence 5'-YTCGAR-3' (Y = @entity7391 ; R = @entity10043 ). M.BsuM was purified from the exponentially growing cells of @entity7682 M. This enzyme (45 +/- 1 kDa) is monomeric and recognizes only double-stranded DNA. It is inhibited partially by @entity1025 , @entity3281 ions and @entity11667 and almost totally by @entity3208 , @entity1133 and agarose. This enzyme methylates specifically the three methylatable sites of the plasmid pBM3. Relaxation of specificity ('star' activity) was observed in the presence of organic solvents. A very low amount of M.BsuM was obtained in the standard Marburg strain. To obtain sufficient enzyme attempts are being made to clone the M.BsuM gene in @entity526 by using a constructed plasmid (pBM14) vector. Only one transformant containing a 3-kb insert and showing a low level of expression, was obtained.
