Effect of dibutyltin on XXXX levels in human natural killer cells.

@entity5 @entity20207 @entity855
This study investigates the role that decreased @entity855 levels may play in dibutyltin ( @entity20207 )-induced @entity5 (lytic) function of natural killer (NK) cells. NK cells are a subset of lymphocytes capable of killing @entity5 cells, virally infected cells, and antibody coated cells. @entity20207 is used as stabilizer in PVC plastics and has also been used as a deworming product in poultry. NK cells were exposed to various concentrations of @entity20207 for 1 h, 24 h, 48 h, and 6 days before determining @entity855 levels and lytic function. @entity855 levels and lytic function were also determined in NK cells that were exposed to @entity20207 for 1 h followed by 24 h, 48 h, and 6 days in @entity20207 -free media. The results indicated that exposure of NK cells to 10 muM @entity20207 for 1 h did not cause any significant decrease in NK cell @entity855 levels but did cause a very significant loss in lytic function. NK cells exposed to 500 nM @entity20207 for 24 h showed significant loss of lytic function but showed no decrease in @entity855 levels. However, 48 h and 6 days exposures to those concentrations of @entity20207 that caused decreases in @entity5 lysing function also caused significant decreases in @entity855 levels. Exposures of NK cells to varying @entity20207 concentrations for 1 h followed by 24 h, 48 h, and 6 days in @entity20207 free media produced effects on lytic function and @entity855 levels that were similar to those seen with continuous @entity20207 exposures. The results indicate that @entity20207 exposures decrease @entity855 levels in NK cells but that @entity5 lysing function can be reduced independent of any decreases in @entity855 levels. Additionally the results show that the effects of a range of @entity20207 concentrations on @entity855 levels and @entity5 lysing function are irreversible.
