Integrity of acquired chromosomal alterations during the metastatic progression of human XXXX detected by high-density comprehensive allelotype analysis.

@entity5 @entity75 @entity1600 @entity1017 @entity74 @entity3 @entity157
UNASSIGNED: 9551 Background: It is widely accepted that chromosomal instability is one of the essential features of @entity5 cells and that significant intratumoral heterogeneity based on the genetic changes may be found in many type of @entity5 . Therefore, different metastatic lesions in an individual case may well show different genetic changes, reflecting the heterogeneity of the @entity5 , and may serve an important information regarding to the clonal evolution of the @entity5 . METHODS: Tumor tissues of multiple metastatic lesions as well as @entity5 tissues were obtained from two autopsy cases with @entity157 ( @entity157 ) with informed consent. Extracted DNA was subjected to comprehensive allelotype analysis using 400 microsatellite markers that distribute throughout 22 autosomes and X chromosome. Furthermore, the alleles showing imbalance were subjected to comparative multiplex PCR, using a retained allele as an internal control, to determine whether the imbalance was the result of @entity1017 . RESULTS: In the first case, @entity74 detected in the various metastatic lesions, including two macroscopically distinct lesions of main @entity5 , intrahepatic @entity3 , @entity1600 , pulmonary @entity3 and portal vein thrombus (PVT), were almost identical, showing -1p, +1q, -4q, +7, -8p, +8q, +9q, +10, -13q, -17p, +19p, -19q and -X. Additional alterations of +2q and -16q were detected in the PVT and the one portion of the main @entity5 . In the second case, @entity74 detected in three @entity75 and pulmonary @entity3 were completely identical, showing +1, -2q, +4p, -4q, -5, -8p, +8q, +9, -11q, +12, -13q, -14q and -17p. CONCLUSIONS: The rate at which chromosomal aberrations accumulate in @entity157 is significantly slow, retaining the acquired chromosomal alterations during the metastatic progression of the @entity5 . No significant financial relationships to disclose.
