A new method to measure intestinal secretion using @entity2822 -inulin in small bowel of XXXX .

@entity63 @entity4358 @entity2822 @entity2823 @entity35 @entity7482 @entity1928
BACKGROUND: Small intestine @entity63 can be seen in various conditions such as intestinal transplantation. To further understand the pathologic disruption in @entity63 -reperfusion injury, we have developed a method to measure fluid changes in the intestinal lumen of  @entity35 . METHODS: Two 10-cm @entity35 intestine segments were procured, connected to the terminal apertures of a perfusion device, and continuously infused with 3  mL of @entity7482 solution (control solution) containing 50   M of @entity2822 ( @entity2823 )-inulin. The perfusion device consists of concentric chambers that contain the perfused bowel segments, which are maintained at 37  C via @entity4358 bath. The individual chamber has four apertures as follows: two fill and/or drain the surrounding @entity7482 solution on the blood side of the tissue. The others provide flow of @entity7482 solution containing @entity2823 -inulin through the lumens. The experimental intestine was infused with the same solution with 100   M of @entity1928 . A pump continuously circulated solutions at 6  mL/min. Samples  were collected at 15-min intervals until 150  min and were measured by the nanoflourospectrometer. RESULTS: A mean of 6- M decrease in the @entity2823 -inulin concentration in the @entity1928 -treated experimental intestine was observed in comparison with that in the control intestine. The @entity2823 -inulin count dilution in the experimental intestine is a result of an increase of fluid secretion produced by the effect of @entity1928 , with P values <0.0001. CONCLUSIONS: We demonstrate that it is possible to measure luminal fluid changes over time using our new modified perfusion system along with @entity2823 -inulin to allow real-time  determinations of fluid and/or electrolyte movement along the small intestine.
