[Analysis of @entity4015 , @entity4016 and @entity20096 gene mutations in XXXX negative @entity5 ].

@entity1 @entity1861 @entity20096 @entity5 @entity32197 @entity4016 @entity4018 @entity1031 @entity4015 @entity4406 @entity4407 @entity4017
OBJECTIVE: To explore the mutational status of @entity4015 , @entity4016 and @entity20096 genes in @entity1861 negative @entity5 ( @entity5 ) @entity1 and the clinical features of @entity5 @entity1 with these mutations. METHODS: A total of 246 @entity1 with a definite diagnosis of @entity1861 negative @entity5 were enrolled from January 2009 to January 2014 into this study. Among them, there were 48 cases of @entity4406 ( @entity4406 ) @entity1 , 171 cases of @entity4407 ( @entity4407 ) @entity1 and 27 cases of @entity4018 ( @entity4018 ) @entity1 . And @entity4015 , @entity4016 @entity4017 , 12 exons of @entity4016 and @entity20096 @entity32197 /K genes were amplified by PCR and sequenced directly. Clinical features were also analyzed in @entity1 . RESULTS: Among 246 cases of @entity1861 -negative @entity5 @entity1 , 52 cases (21.1%) had @entity4015 mutation, 121 cases (49.2%) @entity4016 @entity4017 mutation, 0 case (0) 12 exons of @entity4016 mutation, and 2 cases (0.8%) @entity20096 @entity32197 /K mutation, respectively. These mutations were found existing exclusively. In @entity4406 @entity1 , the white blood cell and platelet counts in @entity4016 @entity4017 mutated group were higher than those in wild-type @entity4016 @entity4017 group, while the level of hemoglobin was higher in wild-type @entity4016 @entity4017 group (all P < 0.05). In @entity4407 @entity1 , the white blood cell count, the level of hemoglobin, the frequency of @entity1031 events and risk stratification in @entity4016 @entity4017 mutated group were higher than those in @entity4015 mutated group (all P < 0.05). In @entity4018 @entity1 , the level of hemoglobin in @entity4016 @entity4017 mutated group were significantly higher than those in @entity4015 mutated group (P < 0.05). CONCLUSIONS: The proliferative level of bone marrow, risk of @entity1031 events and stratification are lower in @entity4015 mutated @entity1 than those in @entity4016 @entity4017 mutated @entity1 . The pathogenic mechanism of mutated gene should be further investigated in future.
