Total abdominal XXXX uptake reflects intestinal adenoma burden in Apc mutant @entity19 .

@entity19 @entity652 @entity419 @entity14
Apc mutant (Apc(Min)) @entity19 develop multiple @entity652 in their intestines and are widely used to study @entity14 and chemopreventive approaches. Molecular imaging of intestinal @entity652 could potentially provide noninvasive longitudinal evaluation of these lesions in living @entity19 . Therefore, the aim of this study was to investigate the role of @entity419 PET in the Apc(Min) @entity19 model. METHODS: Apc(Min) @entity19 (n = 8) fed a purified diet were imaged serially after injection of @entity419 at age 9 and 12 wk using a small-animal PET scanner. Abdominal uptake of the tracer was quantified. After dissection, intestines were imaged separately, and intestinal tracer uptake was quantified. Tracer distribution was compared with results from microscopic examination regarding adenoma number and size. Thereafter, findings were validated serially in 20 Apc(Min) @entity19 aged 6, 8, 10, and 12 wk that received standard chow to increase @entity652 . In vivo abdominal @entity419 uptake was correlated with microscopy results. RESULTS: Microscopic examination showed that the @entity19 developed 25-35 intestinal @entity652 at age 12 wk. Ex vivo @entity419 PET of the dissected intestines visualized all large @entity652 and most small @entity652 . Ex vivo total intestinal @entity419 uptake correlated with in vivo total abdominal uptake and with the number of large @entity652 at age 9 and 12 wk. At 12 wk, there was a clear correlation between in vivo abdominal tracer uptake and the number of large @entity652 but not the total number of lesions. CONCLUSION: Intestinal @entity652 in Apc(Min) @entity19 are metabolically active lesions that take up @entity419 . Abdominal @entity419 uptake at age 12 wk serves as a readout modality for large intestinal @entity652 .
