@entity519 Preconditioning Increases Survival and Pro-Angiogenic Capacity of XXXX Cord Blood Mesenchymal Stromal Cells In Vitro.

@entity1 @entity31373 @entity3274 @entity39 @entity63 @entity1836 @entity7 @entity584 @entity519 @entity842 @entity880
UNASSIGNED: @entity519 preconditioning was shown to improve the therapeutic efficacy of bone marrow-derived multipotent mesenchymal stromal cells (MSCs) upon transplantation in @entity63 tissue. Given the interest in clinical applications of umbilical cord blood-derived MSCs, we developed a specific @entity519 preconditioning protocol and investigated its anti-apoptotic and pro-angiogenic effects on cord blood MSCs undergoing simulated @entity63 in vitro by subjecting them to @entity519 and nutrient deprivation with or without preceding @entity519 preconditioning. Cell number, metabolic activity, surface marker expression, chromosomal stability, apoptosis (caspases-3/7 activity) and @entity39 were determined, and phosphorylation, mRNA expression and protein secretion of selected apoptosis and angiogenesis-regulating factors were quantified. Then, @entity1 umbilical vein endothelial cells (HUVEC) were subjected to simulated @entity63 in co-culture with hypoxically preconditioned or na  ve cord blood MSCs, and HUVEC proliferation was measured. Migration, proliferation and @entity842 production of HUVECs were determined in presence of cord blood MSC-conditioned medium. Cord blood MSCs proved least sensitive to simulated @entity63 when they were preconditioned for 24 h, while their basic behavior, immunophenotype and karyotype in culture remained unchanged. Here, "post- @entity63 " cell number and metabolic activity were enhanced and @entity31373 activity and @entity880 dehydrogenase release were reduced as compared to non-preconditioned cells. Phosphorylation of @entity584 and BAD, mRNA expression of @entity1836 , @entity3274 and @entity7 , and @entity7 protein secretion were higher in preconditioned cells. Hypoxically preconditioned cord blood MSCs enhanced HUVEC proliferation and migration, while @entity842 production remained unchanged. We conclude that @entity519 preconditioning protects cord blood MSCs by activation of anti-apoptotic signaling mechanisms and enhances their angiogenic potential. Hence, @entity519 preconditioning might be a translationally relevant strategy to increase the tolerance of cord blood MSCs to @entity63 and improve their therapeutic efficacy in clinical applications.
