Cryopreserved XXXX amniotic membrane and a bioinspired underwater adhesive to seal and promote healing of iatrogenic fetal membrane defect sites.

@entity1 @entity1707 @entity130 @entity2241
INTRODUCTION: We investigated the ability of cryopreserved @entity1 amniotic membrane (hAM) scaffold sealed with an underwater adhesive, bio-inspired by marine sandcastle worms to promote healing of @entity2241 in a pregnant @entity1707 model. METHODS: Twelve Yucatan miniature @entity1707 underwent laparotomy under general anesthesia at 70 days gestation (term  =  114 days). The gestational sacs were assigned to uninstrumented (n  =  24) and instrumented with 12 Fr trocar, which was further randomized into four different arms-no hAM patch, (n  =  22), hAM patch secured with suture (n  =  16), hAM patch with no suture (n  =  14), and hAM patch secured with adhesive (n  =  9). The animals were euthanized 20 days after the procedure. Gross and histological examination of the entry site was performed for fetal membrane healing. RESULTS: There were no differences in fetal survival, amniotic fluid levels, or dye-leakage from the amniotic cavity between the groups. The fetal membranes spontaneously healed in instrumented sacs without hAM patches. In sacs with hAM patches secured with sutures, the patch was incorporated into the @entity1707 fetal membranes. In sacs with hAM patches without sutures, 100% of the patches were displaced from the defect site, whereas in sacs with hAM patches secured with adhesive 55% of the patches remained in place and showed complete healing (p  =  0.04). DISCUSSION: In contrast to @entity1 , @entity1707 fetal membranes heal spontaneously after an @entity130 and thus not an adequate model. hAM patches became incorporated into the defect site by cellular ingrowth from the fetal membranes. The bioinspired adhesive adhered the hAM patches within the defect site.
