The liposome-incorporating cell wall skeleton of Mycobacterium bovis bacillus Calmette-Gu  in can directly enhance the susceptibility of XXXX cells to lymphokine-activated killer cells through up-regulation of @entity3223 ligands.

@entity1 @entity3223 @entity43782 @entity5 @entity50372 @entity1532 @entity150 @entity1199
OBJECTIVE:     To conduct a preclinical evaluation of the ability of natural killer cells to cytolyze @entity150 cells that were modified to show enhanced expression of @entity3223 ( @entity3223 ) ligands by R8-liposome-bacillus Calmette-Gu  in (BCG)-cell wall skeleton (CWS) treatment. MATERIALS AND METHODS:     The @entity1 cells and @entity1 cells were co-cultured with R8-liposome-BCG-CWS and BCG for 2, 4, or 6 h, and then the surface expression of @entity3223 ligands was analyzed using TaqMan real-time quantitative RT-PCR.     Peripheral blood mononuclear cells were obtained with a conventional preparation kit, and then lymphokine-activated killer (LAK) cells were generated from these purified peripheral blood mononuclear cells via @entity1199 stimulation.     The anti- @entity5 effect of LAK cells against untreated and R8-liposome-BCG-CWS co-cultured with cells of the @entity1 @entity150 cell lines T24 and RT-112 was analyzed using the cytotoxic WST-8 assay method at 4 h of culture at various effector/target (E : T) ratios. RESULTS:     Major histocompatibility complex class I-related chain B ( @entity1532 ) expression was increased    1.5-fold on @entity1 cells and @entity1 cells with BCG.     @entity50372 expression was also increased    1.5-fold on @entity1 cells and @entity1 cells with BCG. R8-liposome-BCG-CWS increased the surface expression of @entity1532 2.2-fold on @entity1 cells but did not increase it significantly on @entity1 cells.     @entity50372 expression was increased    2.2-fold on @entity1 cells, although no differences were observed between the expression of @entity43782 and 3 with R8-liposome-BCG-CWS.     @entity1 cells that were co-cultured with R8-liposome-BCG-CWS showed an    1.3-fold increase in sensitivity to cytolysis by LAK cells at an E : T ratio of 4 and @entity1 cells showed an    1.4-fold increase at an E : T ratio of 2. CONCLUSIONS:     In the present study, the induction of surface @entity3223 ligands by R8-liposome-BCG-CWS rendered @entity5 cells more susceptible to cytolysis by LAK cells.     @entity1 cells and @entity1 cells, even when cultured singly in the absence of immune cells, can directly respond to R8-liposome-BCG-CWS.     The results obtained in the present study may therefore indicate a novel adoptive immunotherapy against @entity150 .
