Removal of LDL from plasma by adsorption reduces adhesion molecules on mononuclear cells in XXXX with arteriosclerosis obliterans.

@entity1 @entity650 @entity1356 @entity310 @entity1294 @entity3138 @entity1759 @entity1453 @entity731 @entity165 @entity16445
BACKGROUND: There is increasing evidence that immune processes are important in the development of @entity731 . We investigated whether low density lipoprotein (LDL) adsorption therapy affected serum cytokine levels and the expression of adhesion molecules on peripheral blood mononuclear cells (lymphocytes and monocytes) in @entity1 with @entity1294 ( @entity1294 ). METHODS AND RESULTS: LDL adsorption therapy was repeated ten times over a period of three months in ten @entity1294 @entity1 . The total serum @entity165 and LDL @entity165 levels were significantly reduced at the end of therapy. This was associated with a significant improvement in Fontaine's classification and ankle pressure index. We also measured serum levels of inflammatory cytokines ( @entity1356 ( @entity1356 ), @entity650 and tissue necrosis factor alpha ( @entity310 )) and expression of adhesion molecules (lymphocyte function-associated antigen 1 alpha ( @entity1453 ), LFA-1 beta, @entity3138 , very late antigen (VLA)-4, @entity16445 and @entity1759 ) on mononuclear cells in the same @entity1 and a group of healthy subjects. Serum levels of all inflammatory cytokines were markedly higher in @entity1294 @entity1 compared with healthy subjects, but there was no significant difference in the level before and after LDL adsorption. VLA-4 expression on CD3+ cells, but not of other adhesion molecules, was markedly higher in @entity1294 @entity1 compared with healthy subjects. LDL adsorption caused a significant reduction in @entity3138 , VLA4 and @entity16445 expression on CD3+ cells. Furthermore, VLA-4 and @entity16445 expression on monocytes diminished significantly after LDL adsorption. CONCLUSIONS: Our results indicate that LDL adsorption-induced immunoregulation is mediated by an indirect stimulatory effect on the immune system. The results suggests that improved peripheral circulation produced by LDL adsorption may reflect improved immune dysfunctions of @entity731 in @entity1294 @entity1 .
