XXXX nuclear magnetic resonance spectral changes in obstructed or dehydrated kidney.

@entity2700 @entity3791 @entity1391 @entity35 @entity1876 @entity45031
@entity35 kidneys subjected to @entity1876 in vivo both develop marked increases in the area under a peak resonating in the phosphodiester region on their @entity45031 nuclear magnetic resonance spectra. The chemical species responsible for these changes were assessed using physiologic manipulations which altered the concentration of @entity1391 in the urine or increased the urine pH. Obstructed kidneys of @entity35 fed a normal diet had a 140 +/- 50% increase in a peak resonating at 3.31 +/- 0.05 ppm after three hours of obstruction (P less than 0.01). Low @entity1391 diet which decreased urine @entity1391 concentration by 98% virtually eliminated this increase in peak area, where saline diuresis which decreased urine @entity1391 concentration by 50% markedly attenuated it. Acute @entity1391 loading which doubled urine @entity1391 concentration markedly accentuated the increase in peak area. Alkalinizing the urine with @entity3791 (changing urine pH from 6.2 +/- 0.2 to 8.0 +/- 0.1) shifted the resonance frequency of this increasing peak from 3.31 +/- 0.06 to 5.45 +/- 0.11 ppm (P less than 0.01). @entity35 fed a normal diet developed increases (57 +/- 15%, P less than 0.05) in a peak resonating at 2.84 +/- 0.03 ppm following 48 hours of @entity2700 . @entity35 fed a low @entity1391 diet had a comparable increase in the relative area of this peak (46 +/- 16%, P less than 0.05). Alkalinization of the urine did not affect the position or intensity of this peak under conditions of @entity2700 .(ABSTRACT TRUNCATED AT 250 WORDS)
