[Stimulation of adhesion molecule expression in @entity1 vascular endothelium by @entity2868 toxins--influence of XXXX ].

@entity1 @entity2868 @entity4725 @entity4859 @entity6414 @entity3971 @entity4105 @entity2071
The aim of this study was to examine the influence of @entity6414 on the level of expression of adhesion molecules @entity4105 , @entity2071 , and @entity4725 on @entity1 vascular endothelium activated with @entity2868 endotoxins or enterotoxin. Lipopolysaccharides were extracted by @entity3971 -water method from one nonenterotoxigenic (NTBF) and three enterotoxigenic (ETBF) @entity2868 strains. LPS preparations were purified with nucleolytic enzymes and ultracentrifugation. Enteotoxin (BFT) was prepared from the supernatant of reference @entity2868 ATCC 43858 culture by precipitation with @entity4859 . BFT preparations were purified with the application of ion-exchange chromatography and hydrophobic chromatography. Adhesion molecule expression on the surface of @entity1 vascular endothelial cells (HMEC-1 cell line) was determined after simultaneous stimulation with bacterial compounds at the concentration of 10 micrograms/ml and @entity6414 at the concentration of 20 micrograms/ml. Endothelial cells were activated for 4 hours ( @entity4105 expression) or for 24 hours ( @entity2071 and @entity4725 expression). Adhesion molecules were detected in immunoenzymatic test (ELISA) with the use of mouse, monoclonal antibodies against @entity1 @entity2071 , @entity4725 , and @entity4105 . The results of performed experiments suggest, that @entity6414 changes the level of adhesion molecule expression on @entity1 vascular endothelium. This antibiotic causes changes in the expression of endothelial @entity2071 , @entity4725 , and @entity4105 during simultaneous stimulation of endothelium with @entity2868 endotoxins or enterotoxin. In the majority of cases the addition of @entity6414 leads to the up-regulation of examined adhesion molecules.
