Next Generation XXXX Nanoparticles for Efficient Gene Therapy.

@entity137 @entity0 @entity12279 @entity98 @entity8681 @entity505 @entity496
UNASSIGNED: In a pursuit to develop a commercially exploitable and traceable gene delivery vehicle, here, we develop next generation @entity505 nanoparticle-DNA complex (CNPLex). CNPLexes were used to transfect green fluorescent protein (GFP) reporter gene containing plasmid DNA (pDNA) pEGFP-N1 targeting @entity0 cells MCF-7 and MDA-MB231. Prepared CNPs were optimized for particle size, surface potential, polymer surface decoration, absorbance efficiency, fluorescence efficiency, IR spectroscopic signatures, and DNA loading and release efficiencies. Rigorous biophysical methods were employed to determine the variations in physicochemical properties of CNPs after surface decoration with polymers followed by complexation with pDNA. Optimized CNPLexes were used to deliver pEGFP-N1 plasmid and efficiency of GFP was followed by fluorescence microscopy and quantified by flow assisted cell sorting. @entity496 was used as positive control according to manufacturer's protocol and found to be comparative in transfection efficiency with one of our novel formulations. Further evaluation of cell @entity137 and cell viability was performed by LDH activity and @entity98 assay, respectively. It was found that cell @entity137 furnished by polymer decorated @entity505 nanoparticles was significantly low compared to the parent polymer ( @entity12279 , @entity8681 ). Similarly cell viability was found to be much higher with CNPLexes compared to @entity8681 alone. This established the developed particles as better transfecting agents for reporter gene plasmid pEGFP-N1 compared to @entity8681 and showed similar efficacy to one of the best known commercial transfection agents Liofectamine2000 in @entity0 cells MCF-7 and MDA-MB231.
