Isolation and characteristics of XXXX  -/A2B5+ and @entity16533  -/A2B5  - cells from the SHG139s cell line.

@entity11 @entity16533 @entity19 @entity5
UNASSIGNED: In @entity11 tissues, there are small cell populations with the capability of sustaining @entity5 formation. These cells are referred to as @entity11 stem cells (GSCs). However, the presence of subpopulations of GSCs, and the differences between each subpopulation remain to be fully elucidated. In the present study, @entity16533  -/A2B5  - and @entity16533  -/A2B5+ cells from the SHG139 GSC cell line (SHG139s) were isolated using magnetic  -activated cell sorting. Following xenografting into @entity19 , the two isolated subpopulations generated @entity5 . The characteristics of the two subpopulations were investigated extensively, and it was found that the two exhibited @entity5 stem cell characteristics. These cells expressed stem cell markers, exhibited a neurosphere  -like appearance, and were found to exhibit self  -renewal and multipotency capabilities. Subsequently, the self  -renewal and proliferation abilities of the two subpopulations were compared. It was found that the A2B5  - cells had a higher proliferative index and a higher self  -renewal ability, compared with the A2B5+ cells. In addition, the A2B5  - cells exhibited increased angiogenic ability. However, the invasion ability of the A2B5+ cells was higher than that of the A2B5  - cells. Taken together, the results of the present study suggested that there are different cell subpopulations in GSCs, and each subpopulation has its own properties.
