Modification of neutrophil function by plasma of @entity309 XXXX treated with infliximab.

@entity1 @entity650 @entity310 @entity224 @entity309
OBJECTIVE: To examine whether the release of @entity224 anions from neutrophils of healthy donors was affected when incubated with plasma from infliximab-treated @entity309 ( @entity309 ) @entity1 . METHODS: Fifteen consecutive @entity309 @entity1 were treated with 3mg/kg infliximab on weeks 0, 2, 6, and 14. Disease activity was assessed by DAS28 score and by @entity650 level. Neutrophils from healthy donors were incubated with plasma drawn before each infliximab treatment. PMA-stimulated @entity224 release was measured by the ferricytochrome C reduction method. RESULTS: 53% of the @entity1 had a favorable clinical response. @entity650 levels showed a significant decline at week two, with a gradual increase thereafter. Treatment with infliximab did not change the @entity224 production. However, when the group was divided retrospectively to responders (DeltaDAS28 > -1.2) and non-responders (DeltaDAS28 < -1.2), two different patterns were seen, although the pre-treatment levels were similar: Among the responders @entity650 remained low at its 2 weeks level till week 14, while in the non responders @entity650 increased 3 times (P < 0.03) from week 2 to 14. The responders showed mild, but continuous, reduction of @entity224 release, while in the non-responders it increased significantly from week 2 on. CONCLUSION: The reduction in @entity650 in @entity309 sera following anti- @entity310 therapy has little influence on the capacity of these sera to stimulate healthy neutrophils to produce @entity224 , suggesting the existence of non- @entity310 non- @entity650 dependent neutrophil-stimulating mediators in @entity309 sera. The increasing level of @entity650 among the non-responders after initial dramatic decline might represent an escape phenomenon, possibly caused by alternative mediator(s). Clinically, this @entity650 "escape" might be used as a tool for early identification of responders from non-responders.
