Characterization of lymphocytic infiltrates in normal, preneoplastic, and neoplastic XXXX mammary tissues.

@entity1 @entity0 @entity19 @entity5 @entity133
Lymphocytic infiltrates were isolated from normal, preneoplastic, and neoplastic @entity19 mammary tissues. The surface markers on the infiltrating lymphocytes were characterized by immunofluorescent staining and flow cytometry. Preneoplastic and neoplastic tissues contained 10- to 20-fold more in situ lymphocytes than did the normal pregnant gland. Most of these lympocytes were T-cells. Relative to the T-cells in normal gland, the T-cells in C4 preneoplastic @entity133 and their spontaneous @entity5 have shifted in favor of the killer-suppressor subpopulation. This shift of T-cell subpopulations was a localized phenomenon and was not seen in the lymph nodes of @entity133 and @entity5 bearing @entity19 . C4 lesion infiltrating cells also contained a subpopulation of lymphocytes that expressed 5- to 6-fold more LFA-1 antigen (lymphocyte function associated antigen-1) than did normal lymph node cells. The infiltrating lymphocytes of @entity5 from cloned cell lines, on the contrary, had the same staining profile as did the lymphocytes from normal gland. Since most studies with @entity1 @entity0 have demonstrated increased killer/suppressor T-cells and the presence of activated lymphocytes (J. Hurlimann and P. Saraga, Int. J. @entity5 , 35: 753-762, 1985; H.L. Whitwell, H.P.A. Hughes, M. Moore, and A. Ahmed, Br. J. @entity5 , 49: 161-172, 1984; and J.A. Ledbetter, R.V. Rouse, H. Spedding Micklem, and L. Herzenberg, J. Exp. Med., 152: 280-295, 1980) the C4 @entity133 and spontaneous @entity5 system may be a more relevant model for studying @entity0 .
