@entity1799 status in cervical XXXX cell DNA assayed by multiplex PCR.

@entity1 @entity5 @entity72319 @entity189 @entity1799 @entity1082 @entity6860 @entity39174
Integration of @entity1082 ) DNA into host genome occurs early in @entity5 development and is probably an important event in @entity5 . The HPV genome integration usually disrupts E2 gene open reading frames. It results in the lack of E2 gene suppressor of the synthesis of E6 and E7 products which, in turn, leads to the overexpression of E6 and E7 genes. The oncogenic HPV types ( @entity1799 , -18, -45, and -58) can be present as episomes or may integrate into @entity1 chromosomes. Sixty-six cervical @entity5 @entity1 positive for @entity1799 were tested for the presence of @entity72319 genes. Multiplex PCR was carried out in all cases. Using cluster analysis, the calculated ratios of @entity6860 /(E1*E6) gene amplification products were divided into two or three statistically different groups. These were used for statistical analysis of the prevalence of specific gene types in histological types of @entity5 , different levels of clinical staging, and histologically confirmed nodal metastases. The statistical analysis proved a significant correlation in the ratios of @entity39174 only. The @entity39174 were higher in @entity189 in situ than in advanced @entity5 . The E2/E6 ratios were lower in higher clinical stages. The multiplex PCR estimation of the E2/E6 ratio could be a simple method for selecting @entity1 with a high risk of a poor outcome in a standard stage-dependent treatment procedure.
