Mechanical stretch regulates cell survival in XXXX bladder smooth muscle cells in vitro.

@entity1 @entity2667 @entity1184 @entity535 @entity1457 @entity442 @entity1839 @entity150 @entity2533 @entity2182 @entity1014
Our understanding of the pathophysiology of the @entity2182 is poor. It has been proposed that localized contractions result in the abnormal stretching of bladder smooth muscle. We hypothesize that stretch regulates the cellular processes that determine tissue size. The purpose of this study was to investigate the effect of stretch on apoptosis, proliferation, cell @entity1184 , and growth factor production in @entity1 bladder smooth muscle cells in vitro. Normal @entity1 detrusor muscle was obtained from @entity1 undergoing radical cystectomy for invasive @entity150 , and primary cultures were established. Cells were mechanically stretched on flexible plates at a range of pressures and times. Apoptosis was assessed by @entity2667 incorporation and flow cytometry. Radiolabeled @entity1014 and @entity535 incorporation were used to assess proliferation and cell @entity1184 . ELISA and RT-PCR were used to assess growth factor production. Mechanical stretch inhibits apoptosis in a time- and dose-dependent manner and was associated with increases in the antiapoptotic proteins @entity1457 protein-70 and @entity1839 . Stretch also increases smooth muscle cell proliferation and @entity1184 , but @entity1184 is the more dominant response. These changes were associated with increases in @entity442 and basic FGF and a decrease in @entity2533 . Mechanical stretch regulates apoptosis, proliferation, and cell @entity1184 in @entity1 bladder smooth muscle cells.
