Stimulated expression of XXXX in porcine heart after bypass circulation and @entity1167 .

@entity1707 @entity5032 @entity2918 @entity17853 @entity519 @entity56132 @entity1167 @entity13559 @entity13560
OBJECTIVES: @entity13559 ( @entity56132 ) and @entity13560 ( @entity17853 ) enzymes catalyze the initial step in the formation of @entity2918 , which have a role in the regulation of circulation and in inflammatory reactions. As @entity519 is reported to stimulate the expression of @entity17853 , we have investigated the effects of bypass circulation and @entity1167 on the expression @entity56132 and @entity17853 in the myocardium of porcine hearts. METHODS: Anaesthetized @entity1707 were connected to cardiopulmonary bypass and the hearts were arrested by @entity5032 for 30 min and reperfused thereafter for 90 min. Then the mRNA and protein levels of @entity56132 and @entity17853 were measured from the transmural specimens of the left ventricular myocardium by Northern and Western blot analyses. Reference specimens were from the hearts of unoperated control @entity1707 and from sham-operated @entity1707 , which were connected to cardiopulmonary bypass for 120 min without any aortic clamping. RESULTS: @entity56132 mRNA was expressed in unoperated control porcine hearts, whereas the expression of @entity17853 mRNA was weak in control hearts. The expression of @entity17853 mRNA increased to 170% of the control level in the hearts of sham-operated @entity1707 and to 180% in arrested hearts, while the level of @entity56132 mRNA was not changed. Both @entity56132 and @entity17853 proteins were detected by Western blot analysis in the myocardial specimens of control hearts. After @entity1167 , the level of @entity17853 protein increased to 280% of the control level in arrested hearts, whereas the level of @entity56132 protein remained unchanged. CONCLUSIONS: These results indicate that the expression of the @entity17853 gene is stimulated in the ventricular myocardium of the porcine heart after bypass circulation and @entity1167 .
