Keratinocyte induced @entity6722 in the pathogenesis of XXXX .

@entity1 @entity6722 @entity0 @entity4098 @entity52120
In @entity4098 , the epidermis contains large clear neoplastic cells. To explain the pathogenesis of this disease, the immunohistochemical characteristics of these cells were investigated in 25 @entity1 . The cytoplasmic presence of low molecular weight cytokeratin and the absence of high molecular weight cytokeratin in all cases confirmed the glandular origin of the Paget cells. Membrane over-expression of the @entity52120 -protein was established in 96% of cases. It was hypothesized that epidermal keratinocytes release a chemotactic factor which attracts @entity52120 -over-expressing @entity0 cells by @entity6722 into the epidermis. The biological assays showed that normal keratinocytes release one or more chemotactic factor(s) into their conditioned medium, which induced spreading and motility of @entity52120 -over-expressing SK-BR-3 @entity1 @entity0 cells. The conditioned medium of keratinocytes also attracted the SK-BR-3 cells by @entity6722 in a modified Boyden chamber. Furthermore, MCF-7 @entity1 @entity0 cells, which do not over-express the @entity52120 -protein, were not attracted by @entity6722 of conditioned medium of @entity1 keratinocytes. The involvement of the @entity52120 -protein in spreading, motility and @entity6722 is further indicated by the inhibition of these processes by monoclonal antibodies against the extracellular domain of the @entity52120 -protein. We conclude, therefore, that the Paget cells spread through the epidermis due to the motility induced by a chemotactic factor, which is released by epidermal keratinocytes and whose influence is mediated by the @entity52120 -protein.
