Early transcriptional defense responses in XXXX cell suspension culture under high-light conditions.

@entity2271 @entity30861 @entity958 @entity26 @entity2767 @entity21184 @entity273 @entity224 @entity3917
The early transcriptional defense responses and reactive @entity26 species (ROS) production in @entity958 ( @entity958 ) cell suspension culture (ACSC), containing functional chloroplasts, were examined at @entity3917 ( @entity3917 ). The transcriptional analysis revealed that most of the ROS markers identified among the 449 transcripts with significant differential expression were transcripts specifically up-regulated by singlet @entity26 ( @entity2767 ). On the contrary, minimal correlation was established with transcripts specifically up-regulated by @entity224 radical or @entity273 . The transcriptional analysis was supported by fluorescence microscopy experiments. The incubation of ACSC with the @entity2767 sensor green reagent and @entity21184 showed that the 30-min- @entity3917 -treated cultures emitted fluorescence that corresponded with the production of @entity2767 but not of @entity273 . Furthermore, the in vivo photodamage of the D1 protein of photosystem II indicated that the photogeneration of @entity2767 took place within the photosystem II reaction center. Functional enrichment analyses identified transcripts that are key components of the ROS signaling transduction pathway in plants as well as others encoding transcription factors that regulate both ROS scavenging and water deficit stress. A meta-analysis examining the transcriptional profiles of mutants and hormone treatments in @entity958 showed a high correlation between ACSC at @entity3917 and the fluorescent mutant family of @entity958 , a producer of @entity2767 in plastids. Intriguingly, a high correlation was also observed with ABA deficient1 and more axillary growth4, two mutants with defects in the biosynthesis pathways of two key (apo)carotenoid-derived plant hormones (i.e. @entity2271 and @entity30861 , respectively). ACSC has proven to be a valuable system for studying early transcriptional responses to @entity3917 stress.
