Circulating hematopoietic stem cells and putative intestinal stem cells in XXXX .

@entity1 @entity1593 @entity66 @entity16 @entity33 @entity2280
BACKGROUND: The intestinal stem cells (ISC) modulation and the role of circulating hematopoietic stem cells (HSC) in @entity66 ( @entity66 ) are poorly understood. Our aim was to investigate the longitudinal modifications in peripheral blood HSC traffic and putative ISC density induced by @entity1593 ( @entity1593 ) in @entity66 . METHODS: Thirty-one @entity66 @entity1 and 7 controls were enrolled. Circulating CD133(+) and @entity2280 (+) HSC were measured by flow cytometry, at enrolment and after 7  days and 1, 3, 6, 12, and 24  months of @entity1593 . Endoscopy was performed at diagnosis and repeated at 6, 12, and 24  months following @entity1593 . We used the Marsh-Oberhuber score to evaluate the histological severity of @entity16 ; immunohistochemistry was employed to measure the intraepithelial lymphoid infiltrate (IEL, CD3(+) lymphoid cells) and the putative ISC compartment (CD133(+) and Lgr5(+) epithelial cells). RESULTS: At enrolment, circulating HSCs were significantly increased in @entity66 @entity1 and they further augmented during the first week of @entity1593 , but progressively decreased afterwards. @entity66 @entity1 presented with villous atrophy, abundant IEL and rare ISC residing at the crypt base. Upon @entity1593 , IEL progressively decreased, while ISC density increased, peaking at 12  months. After 24  months of @entity1593 , all @entity1 were asymptomatic and their duodenal mucosa was macroscopically and histologically normal. CONCLUSIONS: In active @entity66 @entity1 , the ISC niche is depleted and there is an increased traffic of circulating HSC versus non-coeliac subjects. @entity1593 induces a precocious mobilization of circulating HSC, which is followed by the expansion of the local ISC compartment, leading to @entity33 and clinical remission.
