In vitro and in vivo analysis of antimicrobial agents alone and in combination against multi-drug resistant XXXX .

@entity32 @entity1214 @entity548 @entity19 @entity6414 @entity4509 @entity358 @entity1215 @entity3064 @entity9001
OBJECTIVE: To investigate the in vitro and in vivo antibacterial activities of @entity9001 and other 13 common antimicrobial agents, alone or in combination, against multi-drug resistant @entity3064 . METHODS: An in vitro susceptibility test of 101 A. baumannii was used to detect minimal inhibitory concentrations (MICs). A @entity19 @entity358 model of multi-drug resistant A. baumannii, established by the ultrasonic atomization method, was used to define in vivo antimicrobial activities. RESULTS: Multi-drug resistant A. baumannii showed high sensitivity to @entity9001 (98% inhibition), @entity6414 (78.2% inhibition), and @entity1214 (74.2% inhibition). However, the use of these antimicrobial agents in combination with other antimicrobial agents produced synergistic or additive effects. In vivo data showed that white blood cell (WBC) counts in drug combination groups C ( @entity1214 + @entity4509 ) and D ( @entity1214 + @entity1215 ) were significantly higher than in groups A ( @entity9001 ) and B ( @entity6414 ) (P < 0.05), after administration of the drugs 24 h post-infection. Lung tissue @entity32 gradually increased in the model group during the first 24 h after ultrasonic atomization infection; vasodilation, congestion with @entity548 were observed 48 h post infection. After 3 days of anti-infective therapy in groups A, B, C, and D, lung tissue @entity32 in each group gradually recovered with clear structures. The mortality rates in drug combination groups(groups C and D) were much lower than in groups A and B. CONCLUSION: The combination of @entity1214 with either @entity1215 or @entity4509 is more effective against multi-drug resistant A. baumannii than single-agent @entity9001 or @entity6414 . In addition, the @entity19 @entity358 by ultrasonic atomization is a suitable model for drug screening and analysis of infection mechanism.
