Stimulated neutrophils evoke signal transduction to increase vascular permeability in XXXX lungs.

@entity358 @entity11009 @entity35 @entity11265 @entity1589
The mechanisms by which stimulated neutrophils (PMNs) @entity358 were investigated using twenty-four perfused lung preparations isolated from @entity35 . We tested the ability of unstimulated and mechanically stimulated PMNs to adhere to pulmonary endothelial cells and, thereby, alter pulmonary vascular permeability (measured as the pulmonary filtration coefficient) and hemodynamics. To stimulate PMNs, they were gently agitated in a glass vial for 10 seconds. Perfusing lungs with the stimulated PMNs (stimulated group) elicited a 3-fold increase in the filtration coefficient as compared to lungs perfused with unstimulated cells (unstimulated group). This increase in filtration was completely blocked by preincubation of stimulated PMNs with @entity11265 monoclonal antibody (MoAb group). This increase in filtration coefficient was also completely blocked by @entity11009 , a protein kinase C inhibitor (GF group). Pulmonary vascular resistance increased when the stimulated PMNs were injected to the isolated lungs. Although, preincubation of stimulated PMNs with @entity11265 MoAb successfully blocked and @entity11009 partly blocked this increase in pulmonary vascular resistance. The accumulation of stimulated PMNs within the lungs, as assessed by @entity1589 ( @entity1589 ) levels, was blocked by preincubation of stimulated PMNs with @entity11265 MoAb. However, @entity11009 did not decrease @entity1589 levels. These findings suggest that stimulated PMN-induced increases in pulmonary vascular filtration, resulted from endothelial cell injury caused by adhesion to the endothelial cells, evoke intracellular signaling within the endothelial cells.
