A composite tissue-engineered @entity825 using XXXX nasal chondrocyte and epithelial cells.

@entity825 @entity742 @entity19 @entity705 @entity72499 @entity6886 @entity720 @entity6619 @entity2383
This study evaluates the feasibility of producing a composite engineered tracheal equivalent composed of cylindrical @entity2383 with lumens lined with nasal epithelial cells. Chondrocytes and epithelial cells isolated from @entity742 nasal septum were cultured in Ham's F12 media. After 2 wk, chondrocyte suspensions were seeded onto a matrix of @entity6886 . Cell-polymer constructs were wrapped around @entity6619 tubes and cultured in vitro for 1 wk, followed by implanting into subcutaneous pockets on the backs of @entity19 . After 6 wk, epithelial cells were suspended in a hydrogel and injected into the embedded @entity705 following removal of the @entity6619 tube. Implants were harvested 4 wk later and analyzed. The morphology of implants resembles that of native @entity742 @entity825 . H_E staining shows the presence of mature cartilage and formation of a pseudo-stratified columnar epithelium, with a distinct interface between tissue-engineered cartilage and epithelium. Safranin-O staining shows that tissue-engineered cartilage is organized into lobules with round, angular lacunae, each containing a single chondrocyte. @entity72499 and @entity720 contents are similar to native cartilage. This study demonstrates the feasibility of recreating the cartilage and epithelial portion of the @entity825 using tissue harvested in a single procedure. This has the potential to facilitate an autologous repair of segmental tracheal defects.
