[Relationship between multidrug resistance in XXXX @entity694 LoVo/Adr cell line and intracellular @entity460 ion concentration].

@entity1 @entity632 @entity1855 @entity3576 @entity98 @entity1891 @entity460 @entity141 @entity694
BACKGROUND _ OBJECTIVE: Based on the findings that @entity460 content increased in multidrug resistant (MDR) cells and that the resistance could be reversed by the @entity460 channel blocker @entity1855 , it has been speculated that @entity141 may have role in drug resistance. This study was designed to investigate the relationship between multidrug resistance in @entity1 @entity694 LoVo/Adr cells and the level of cytoplasmic @entity141 . METHODS: @entity3576 /AM was used to label @entity141 in the cytoplasm and confocal microscope was used to observe the changes of @entity141 concentration. The reverse effect of @entity1855 was determined by @entity98 method. The content of intracellular @entity632 (ADR) was determined by flow cytometry. RESULTS: @entity3576 /AM fluorescent intensities of LoVo and LoVo/Adr cells were 850.45 and 1,495.88, respectively. After treatment with @entity1855 , @entity3576 /AM fluorescent intensities of LoVo and LoVo/Adr cells were 813.25 and 1,284.14, respectively. The level of @entity141 in the cytoplasm of LoVo/Adr cells was significantly higher than that in LoVo cells, and was not significantly changed by @entity1855 . The concentration of ADR was increased by @entity1855 in LoVo/Adr cells. The potentiation folds of @entity632 and @entity1891 by @entity1855 in LoVo/Adr cells were 8.85 and 5.31, respectively. CONCLUSION: The increasement of cytoplasmic @entity460 content might be a characteristic phenotype of MDR LoVo/Adr cells. However, there was no direct relationship between multidrug resistance and cytoplasmic @entity141 concentration.
