Phenotypic variations and dynamic topography of transformed cells in an experimental model of @entity4742 -induced XXXX in male @entity329 .

@entity172 @entity5 @entity4743 @entity47850 @entity329 @entity4742 @entity703
This work explores the phenotypic changes affecting transformed cells in an experimental model of @entity4742 ( @entity4743 )-induced @entity703 in male @entity329 . This @entity172 -induced @entity5 presents an important cytological pleomorphism and its origin remains largely controversial. In order to characterize phenotypic variations during @entity5 progression, the occurrence of seven lineage markers was analysed by a morphometric approach in kidney sections of @entity4743 -exposed hamsters (6-11 months). S100 protein, neuron-specific enolase (NSE) and @entity47850 are expressed by a large percentage of malignant cells during @entity5 development. Glial fibrillary acidic protein (GFAP), protein gene product 9.5 (PGP 9.5) and desmin are mostly evidenced in advanced @entity5 whereas Leu 7 always presents a focal expression. As evidenced by double-label immunofluorescence, the coexpression of three important neuroectodermal lineage markers (S100, NSE and PGP 9.5) in earliest @entity5 buds points to a peripheral nerve sheath origin for this @entity5 thus confirming previously published data. For each marker, the fluctuations of expression levels during @entity5 progression as well as the spatial heterogeneity of distribution suggest variable phenotypic differentiation of transformed cell populations. This observation is largely corroborated by double-label immunofluorescence showing coexpression modification of several markers during @entity5 progression. This points to a complex dynamic and spatial self-organization of different phenotypes within @entity5 . Altogether, these results support the concept that @entity4743 -induced @entity703 are not made of unstructured cell populations but represent adaptive complex dynamic biosystems.
