XXXX Cell Lines: Where do they come from? (An American Ophthalmological Society Thesis).

@entity1 @entity10162 @entity5 @entity10159 @entity2184 @entity13687
PURPOSE: To determine whether some of the most often used @entity2184 cell lines resemble their original @entity5 . METHODS: Analysis of the literature, @entity1 charts, histopathology, mutations, chromosome status, HLA type, and expression of melanocyte markers on cell lines and their @entity5 . We examined five cell lines and the @entity5 from which they were derived. RESULTS: Four of the five examined @entity5 were unusual: one occupied the orbit, two were recurrences after prior irradiation, and one developed in an eye with a @entity13687 . One cell line did not contain the @entity10159 mutation, but it was present in the @entity5 . Three of the @entity5 had monosomy 3 (two of these lacked @entity10162 expression); however, all five cell lines showed disomy 3 and @entity10162 expression. All of the cell lines had gain of 8q. Two cell lines lacked expression of melanocyte markers, although these were present in the corresponding @entity5 . CONCLUSIONS: All cell lines could be traced back to their original @entity2184 . Four of the five @entity5 were unusual. Cell lines often differed from their @entity5 in chromosome status and melanocyte markers. However, their specific chromosome aberrations and capacity to continue proliferation characterize them as @entity2184 cell lines.
