Sex differences in platelet XXXX and their association with cardiovascular risk factors.

@entity6071 @entity1 @entity650 @entity6800 @entity101 @entity39 @entity5 @entity20040 @entity2938 @entity6438 @entity2842 @entity8729 @entity165 @entity11709 @entity432 @entity715 @entity13976 @entity22879 @entity34617 @entity1851
OBJECTIVE: Platelets contribute to @entity715 , and platelet @entity6071 ( @entity6071 ) are central in pathogen detection, potentially mediating infection-induced @entity432 . Using a large community-based cohort study, we sought to examine if platelets express all known TLR transcripts and analyze their association with cardiovascular risk factors. APPROACH AND RESULTS: mRNA levels for @entity6071 were measured in isolated platelets by high-throughput quantitative reverse transcriptase polymerase chain reaction in 1625 @entity1 (mean age, 66.6  9; 54% @entity1 ) of the Framingham Heart Study. We measured circulating inflammatory and thrombotic markers ( @entity1851 , @entity650 , @entity2842 , intracellular cell adhesion molecule 1, soluble @entity5 @entity39 factor-a receptor 1, and soluble @entity8729 ) and analyzed @entity6071 and their association with sex and cardiovascular risk factors by multivariable logit regression model adjusted for confounding factors. Platelets expressed all 10 TLR transcripts, and all @entity6071 were coexpressed. @entity1 had higher platelet TLR expression, which associated with different cardiovascular risk factors, compared with @entity1 . In @entity1 , @entity11709 , @entity6800 , @entity13976 , and @entity6438 were associated with body mass index and @entity20040 , @entity6438 , and @entity34617 were associated with total @entity165 to high-density lipoprotein ratio. In @entity1 , @entity11709 , @entity2938 , and @entity6800 were associated with lipid and @entity22879 with @entity101 treatment. Similarly, TLR expression in @entity1 was more commonly associated with circulating inflammatory markers (soluble @entity5 @entity39 factor-a receptor 1 and intracellular cell adhesion molecule 1), whereas in @entity1 , TLR expression was associated with soluble @entity8729 levels. CONCLUSIONS: We report the first study to demonstrate that platelets express all TLR transcripts using a large community-based observational cohort. These transcripts are more abundant in @entity1 and have distinct associations with cardiovascular risk and inflammatory biomarkers that vary by sex.
