Effect of hormones on phospholipid metabolism in XXXX cultured fibroblasts.

@entity1 @entity1169 @entity844 @entity2178 @entity2434 @entity3764 @entity2647 @entity3633 @entity112 @entity3044 @entity7544 @entity4357 @entity7609
The effect of hormones on phospholipid metabolism, pool size, 32P labeling and changes in @entity844 of @entity1 adult fibroblasts was determined. Simultaneously the change in membrane fluidity of single cells was recorded via fluorescence recovery after photobleaching under the influence of hormones. From all substances tested ( @entity3044 , @entity2647 , @entity7544 , @entity112 , @entity2178 , @entity3764 , @entity1169 ) only @entity3044 and @entity7544 slightly decreased total amount of @entity2434 (PC). The amount of the other phospholipids analyzed remained unchanged. The 32P incorporation rate into phospholipids (PC, @entity4357 (PI), @entity3633 (PE)) was affected basicly different analyzing either PC, PI or PE. @entity112 and @entity1169 provoked the highest incorporation of 32P (240% increase in PI labeling). @entity3044 and @entity7544 decreased PC labeling (45% and 18%) whereas @entity3044 decreased 32P incorporation into PI (18%), and @entity7544 led to an increase (37%). PE labeling showed no or a slight increase in 32P incorporation applying the other agonists or antagonists. The @entity844 pattern of the respective phospholipids changed only to a minor extend. A decrease in @entity7609 content of PI was found after administration of either @entity3044 , @entity7544 or @entity112 . Parallel determination of membrane fluidity of single cells by fluorescence recovery after photobleaching showed an increase in the diffusion coefficient of a fluorescent lipid probe sticking in the membrane, following administration of @entity3044 and @entity7544 , other substances tested exerted no effect. A relationship to changes in phospholipid metabolism became obvious. These results are discussed considering known mechanisms of receptor coupling and change in phospholipid metabolism and fluidity.
