Airway epithelial cytokine responses in childhood wheeze are independent of XXXX .

@entity1 @entity650 @entity5737 @entity12131 @entity1261 @entity7 @entity1356 @entity310 @entity2842 @entity2843 @entity1495 @entity687 @entity159
BACKGROUND: Airway epithelial cells (AEC) are key contributors to immune function in the lungs but little is known about their role and function in @entity1 . OBJECTIVES: Having previously established that nasal AEC mediator release correlates with that of bronchial AEC, we assessed AEC responses in @entity1 with and without a history of wheeze. METHODS: Nasal AEC cultures were established from @entity1 (0.6-14.9 years) undergoing elective surgical procedures under general anaesthetic categorised as atopic asthmatic (n  =  12), virus-induced wheeze (n  =  8) or @entity1 without wheeze (n  =  32). Mediator release by AEC monolayers at passage 2 was determined by cytometric bead array assay or ELISA. RESULTS: Unstimulated AEC from @entity1 with a history of wheeze produced significantly less @entity687 , @entity650 , @entity2842 and @entity1261 than AEC from healthy controls. There were no group differences in AEC release of @entity7 , @entity5737 , @entity1495 or @entity2843 . After stimulation with the pro-inflammatory cytokines @entity1356 and @entity310 , AEC from @entity1 with current wheeze produced significantly less @entity687 , @entity650 and @entity2842 than @entity1 without wheeze. Release of @entity1261 , @entity7 , @entity1495 and @entity2843 did not differ between the wheeze and control group. There were no differences in mediator release between subjects with @entity12131 and those with virus-induced wheeze or between atopic and non-atopic controls. On multivariate analysis, wheeze was the only significant predictor of AEC mediator release. CONCLUSION _ CLINICAL RELEVANCE: Intrinsic differences in AEC from @entity1 with a history of wheeze may reflect a defect in cytokine production in  vivo or an altered state of differentiation in  vitro, independent of @entity159 .
