Oxidative phosphorylation in XXXX . An uncoupled mutant with altered membrane structure.

@entity859 @entity1025 @entity4588 @entity526 @entity2624 @entity413 @entity855
1. A new mutant strain (AN228) of @entity2624 , unable to couple phosphorylation to electron transport, has been isolated. The mutant allele (unc-405), in strain AN228, was found to map near the uncA and uncB genes at about minute 74 on the @entity526 genome. 2. A transductant strain (AN285) carrying the unc-405 allele is similar to the uncA and uncB mutants described previously in that it is unable to grow on @entity4588 , gives a low aerobic yield on limiting concentrations of @entity413 , has a normal rate of electron transport, is unable to couple phosphorylation to electron transport, and lacks @entity855 -dependent transhydrogenase activity. 3. Strain AN285 (unc-405) is similar to an uncA mutant, but different from an uncB mutant, in that it is unable to grow anaerobically in a @entity413 -mineral-salts medium, and membrane preparations do not have @entity1025 -stimulated @entity859 triphosphatase activity. 4. Strain AN285 (unc-405) does not form an aggregate analogous to the membrane-bound @entity1025 -stimulated @entity859 triphosphatase aggregate found in normal cells. In this respect it differs from strain AN249 (uncA(-)), which forms an inactive membrane-bound @entity1025 -stimulated @entity859 triphosphatase aggregate.
