XXXX is required for timely cell-cycle entry and progression in hepatocytes after acute @entity4314 exposure in @entity19 .

@entity1603 @entity4314 @entity14605 @entity3145 @entity19 @entity4698 @entity14745 @entity30516 @entity99 @entity637 @entity995
Cell-cycle induction in hepatocytes protects from prolonged @entity3145 after @entity99 . @entity14745 (-/-) @entity19 exhibit @entity99 after @entity4314 ( @entity14605 ) exposure and reduced @entity637 production. Because @entity637 is required for prompt cell-cycle induction after @entity99 , here, we tested the hypothesis that @entity14745 is required for timely hepatocyte entry into the cell cycle after @entity14605 -induced @entity99 . @entity99 was induced by a single injection of @entity14605 . Assays were employed to assess indices of the cell cycle in liver after @entity14605 exposure. @entity1603 incorporation peaked in wild-type @entity19 at 48 h after @entity14605 but was reduced by 80% in @entity14745 (-/-) @entity19 . Proliferating-cell nuclear-antigen immunohistochemistry revealed blocks in cell-cycle entry and progression to DNA synthesis in @entity14745 -deficient @entity19 48 h after @entity14605 . @entity4698 D, important for G0/G1 progression, was reduced at baseline and 36 h after @entity14605 . @entity30516 , required for G1/S-phase transition, was reduced in @entity14745 (-/-) @entity19 24 and 48 h after @entity14605 exposure and was associated with reduced phosphorylation of the @entity995 protein. Proliferation in @entity14745 (-/-) @entity19 was delayed, rather than blocked, because indices of cell-cycle progression were restored 72 h after @entity14605 exposure. We concluded that @entity14745 was required for prompt cell-cycle entry (G0- to G1-phase) and G1/S-phase transition after @entity99 . These data support the hypothesis that @entity14745 provides hepatoprotection in the @entity14605 -injured liver, attributable, in part, to timely cell-cycle induction and progression.
