Liver cell reactive components in XXXX fluids.

@entity1 @entity1603 @entity137 @entity535 @entity69 @entity3157 @entity880 @entity413 @entity298
Metabolic changes in @entity298 ( @entity298 ) @entity1 are an important aspect concerning long-term outcome. Liver plays the main role in regulating metabolism. The effects of @entity298 fluids (PDF) on liver cell function are scarcely investigated. Therefore, we investigated the effects of PDF, different in some components, on liver cell metabolism in vitro. Metabolic activity (MTT), cell integrity (LDH release), proliferation ( @entity1603 incorporation) and synthesis of albumin and @entity69 are measured by incubating HepG2 cells for 3 h and 24 h with six different PDFs: (a) @entity880 -buffered, pH5.5: PDF I (1.5% gluc.); PDF II (4.5% gluc. ); (b) @entity3157 -buffered, pH7.4: PDF III (1.5% gluc.), PDF IV (4. 5% gluc.); (c) @entity535 -based solutions, pH 7.4: PDF V (low AA level) and PDF VI (high AA level). Metabolic activity of @entity3157 -treated cells is greatly enhanced in comparison to @entity880 -buffered PDFs. These findings are confirmed by proliferation data. Synthesis of albumin and @entity69 is significantly enhanced by @entity535 -based solutions. Our data demonstrate, that @entity880 -buffered PDF impair liver cells much stronger than @entity3157 -buffered PDF. pH is the parameter which contributes to @entity137 and impaired metabolism to a major extent. In contrast to @entity413 -containing solutions, @entity535 -based PDF stimulate protein synthesis in liver cells.
