Cytomorphological, cytogenetic, and molecular biological characterization of four new XXXX renal carcinoma cell lines of the clear cell type.

@entity1 @entity2214 @entity5 @entity19 @entity984 @entity517 @entity10007 @entity876 @entity1358 @entity1639 @entity2277 @entity10242
Four new permanent cell lines (RCC-A, -B, -C, and -D) derived from different @entity1 @entity1358 of the clear cell type were established in tissue culture. The cell lines displayed characteristic differences in cell size and shape, which allowed individual identification by phase contrast microscopy. Ultrastructurally, the cell lines exhibited varying amounts of cytoplasmatic glycogen and lipid. Immunohistochemistry revealed co-expression of @entity2277 and cytokeratin in all cell lines. The mean population doubling time ranged from 27 h (RCC-A) to 104 h (RCC-D). RCC-B and -C cells produced slowly growing @entity5 after heterotransplantation into @entity19 , whereas RCC-A and RCC-D cells were non-tumorigenic. The modal chromosome number was either near-diploid (RCC-A, -B, and -C) or near triploid (RCC-D). @entity2214 affecting the short arm of chromosome 3 were seen in all cell lines. Northern blot analysis revealed no expression of the proto-oncogenes @entity1639 , c-ros, and @entity10007 , whereas @entity984 expression was observed in all cell lines. Expression of @entity517 was observed in RCC-A, RCC-B, and RCC-D cells, whereas @entity10242 expression could be detected in RCC-B and RCC-D. @entity5 suppressor gene @entity876 mRNA was observed in the cell line RCC-D.
