Plant protein 2- @entity2569 peroxiredoxin TaBAS1 alleviates oxidative and nitrosative stresses incurred during cryopreservation of XXXX cells.

@entity3505 @entity1 @entity2569 @entity3733
UNASSIGNED: There is increasing demand for cryopreserved cells such as liver and pancreatic cells for clinical applications. Cryopreservation at ultra-low temperatures requires use of cryoprotectants (e.g. @entity3733 ( @entity3733 )) to maintain cell integrity during freezing and thawing processes. Standard cryoprotectants are cytotoxic and more effective cryopreservation technologies are urgently needed for long-term storage of cells. As an alternative, soluble protein extracts (WPE) from winter @entity3505 successfully replaced @entity3733 as a cryoprotectant for several @entity1 cell types. To identify novel cryoactive proteins, the WPE was separated by chromatography and cryoactive fractions were analysed by mass spectrometry. The @entity3505 protein 2- @entity2569 peroxiredoxin BAS1 (renamed TaBAS1) was identified as a potential cryoactive candidate. Recombinant proteins were prepared and found to possess dual functions as a peroxidase antioxidant and molecular chaperone, and display cryoprotective properties for hepatocytes and insulin-secreting INS832/13 cells. Following cryopreservation with TaBAS1, cells were plateable and showed high post-thaw viability, good adhesion properties and well-maintained cell-specific metabolic functions. The overall quality of these cell types was equivalent or improved compared to cells that were cryopreserved with @entity3733 . The antioxidant and chaperone functions of TaBAS1 likely explain its efficacy in reducing oxidative/nitrosative stresses in cryopreserved cells. The plant protein TaBAS1 could be a promising molecule to include in cryostorage protocols. This article is protected by copyright. All rights reserved.
