Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from XXXX with @entity583 .

@entity1 @entity7802 @entity14600 @entity2279 @entity2280 @entity583
INTRODUCTION: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from @entity1 with @entity583 ( @entity583 ) proved as good angiogenesis model, we investigated the expression of @entity2280 and @entity2279 as BECs markers, and D2-40, @entity14600 and @entity7802 as LEC markers in this material. MATERIALS AND METHODS: Aspirated thrombi were stained immunohistochemically for @entity2280 , @entity2279 , D2-40, @entity14600 and @entity7802 . Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for @entity2279 , D2-40, @entity14600 and VEGRF3 was done. Thrombi were also graded as fresh (<1day old), lytic (1-5days old) and organized (>5days old). RESULTS: Serial sections of aspirated thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of @entity2279 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. CONCLUSION: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that @entity2280 and @entity2279 on one hand, and D2-40, @entity14600 and @entity7802 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not.
