Observations on the energy and redox state and protein synthetic rate in animal and XXXX placentas.

@entity1 @entity859 @entity742 @entity514 @entity63 @entity840 @entity377 @entity519 @entity880 @entity860 @entity855
The energy and redox states of placentas in newly anesthetized or sacrificed @entity840 and @entity742 were compared with that of @entity1 placentas immediately after delivery. Subsequently the effect of @entity63 was observed in both @entity1 and animal placentas. The influence of perfusion, using a fluid containing oxygenated red cells (adult @entity1 ) was studied in the @entity1 placenta. Adenine @entity377 precursors were added to the perfusion fluid in an attempt to improve the energy and redox states and the mixed protein synthetic rate of the tissue. The @entity855 and @entity880 concentrations in @entity840 placental tissue taken immediately after nembutal anesthesia were 1.16 and 3.59 mmol kg-1 wet weight respectively. One minute's @entity63 caused a fall in @entity855 to 0.7 and a rise in @entity880 to 6.5 mmol kg-1. The concentration of @entity855 in @entity1 placental tissue, 30 secs to 3 minutes after delivery of the organ, was 0.52 and of @entity880 4.8 mmol kg-1 wet weight, suggesting that some biochemical deterioration had already occurred. A further fall in @entity855 to 0.21 and rise in @entity880 to 6.4 mmol kg-1 wet weight took place during 20 mins of @entity63 ; thereafter the @entity855 level remained constant while the @entity880 continued to rise to 11 mmol kg-1 wet weight by 1 1/2 hours. The initial energy charge was similar in both animal and @entity1 placentas, 0.8-0.9, and was only reduced during prolonged @entity519 at 37 degrees C. Since the @entity860 did not rise to the same extent as in other tissues, it is suggested that the Atkinson equation used in the calculation of energy charge may not be applicable to the placenta, and that the most sensitive indication of deterioration in energy status is the @entity514 fall in @entity855 concentration. Rapid establishment of maternal intervillous perfusion of an isolated lobule of the @entity1 placenta using ixygenated RBCS in the perfusion fluid, enabled the @entity855 and @entity880 levels to be maintained near to 'in vivo' values during one hour, with a protein turnover rate of 40% day-1, two thirds of that observed 'in vivo' in the @entity742 . The addition of 1.0-50 microM @entity859 to the perfusion fluid improved the energy state of the tissue, but not its redox state nor protein synthetic rate. 1.5 microM inosine in the perfusion medium did not alter the energy state but caused a marked reduction in @entity880 production.
