[Are centrosomal abnormalities correlated to DNA ploidy in XXXX ?].

@entity16739 @entity0 @entity5 @entity189 @entity513 @entity2823 @entity1558
@entity16739 ploidy was shown to play a role in genomic instability of @entity5 cells and prognosis. The implication of the centrosome in the cell cycle was also described. Therefore, new prognostic factors could be suggested for a better-tailored therapy. The purpose of this study is to search for correlation between centrosomal abnormality and @entity16739 ploidy in @entity0 . Cell prints were prepared from cell culture of mesothelial ascitis, fibroblast cell line MRC5 and @entity0 cell lines MCF7 and T47D. Fresh cell prints were also obtained from cases with @entity189 . The centrosome was labelled by an indirect immunofluorescence assay using anti-y-tubulin antibody and F(ab')(2) @entity2823 before quantification with fluorescence microscopy. @entity16739 ploidy was scored with DNA index obtained by means of flux cytometry. The normal mesothelial cells (94% of cells with only one centrosome) and the diploid cell line MRC5 (68% of cells with two centrosomes) were used as controls. DNA ploidy was found to be correlated with centrosomal abnormality in MCF7 cell line (64% of cells had more than three centrosomes) but not in the 10 cases of @entity513 analysed in this study. The absence of correlation between DNA ploidy and centrosomal abnormality in @entity0 samples may be due to the small numbers of cases, the cell prints or tumorigenesis. Correlation analysis of a larger number of cases and types of @entity1558 of the centrosome are ongoing.
