Esophageal and XXXX epithelial cell proliferation in northern Chinese subjects living in a high-incidence area.

@entity1 @entity1603 @entity4726 @entity1364 @entity1885 @entity1721 @entity1491 @entity418 @entity1613 @entity3621 @entity356 @entity1141
Linxian and the nearby county Huixian, in the Henan province in Northern China, have a very high incidence of @entity1721 (SCC). Previous studies from these counties have suggested that increased proliferation of esophageal epithelial cells, morphologically manifested as @entity1491 ( @entity1491 ) and @entity1141 ( @entity1141 ), is an early indicator of abnormality in @entity1 predisposed to SCC. A high incidence of @entity418 (AC) was also found in these areas. To determine proliferation patterns of esophageal and @entity356 epithelia with normal and different severities of @entity1613 , we measured @entity1364 ( @entity1364 ), Ki-67, and @entity1603 ( @entity1603 ) incorporation and compared the results. Esophageal biopsies (175) and @entity356 biopsies (41) were collected from symptom-free subjects in Huixian. Of these, 23 esophageal biopsies were incubated with @entity1603 . The avidin- @entity1885 -peroxidase complex (ABC) method was used to detect @entity1364 , Ki-67, and @entity1603 . The number of immunostain-positive cells was counted manually. Intense immunostaining for @entity1364 , Ki-67, and @entity1603 was observed in the cell nuclei of tissues with normal and different severities of @entity1613 . With esophageal biopsies, both @entity1364 and Ki-67 increased significantly as the epithelia progressed from normal to @entity1491 and to @entity1141 . The number of @entity1364 - and Ki-67-positive cells was three times higher than that of @entity1603 incorporation in the same category of @entity1491 . With @entity418 biopsies, the number of Ki-67 positive cells was lower in normal tissue and increased significantly from chronic superficial @entity3621 to chronic @entity4726 to @entity1141 . Staining patterns for @entity1364 and Ki-67 were correlated with the histopathology of the esophagus. The correlation was not as clear with @entity356 . @entity1603 studies appear to be more complicated. The @entity1364 and Ki-67 methods may be useful for screening high-risk esophageal and @entity356 subjects, and for monitoring chemoprevention effects.
