Isolation of Mal d 1 and Api g 1 - specific recombinant antibodies from mouse IgG XXXX fragment libraries - Mal d 1-specific antibody exhibits cross-reactivity against Bet v 1.

@entity1 @entity2023 @entity5419 @entity19 @entity1334 @entity56
BACKGROUND: Around 3-5% of the population suffer from IgE-mediated @entity5419 in Western countries and the number of food-allergenic @entity1 is increasing. Individuals with certain @entity2023 may also suffer from a sensitisation to proteins in the food products. As an example a @entity1 sensitised to the major birch pollen allergen, Bet v 1, is often sensitised to its homologues, such as the major allergens of apple, Mal d 1, and celery, Api g 1, as well. Development of tools for the reliable, sensitive and quick detection of allergens present in various food products is essential for @entity56 @entity1 to prevent the consumption of substances causing mild and even life-threatening immune responses. The use of monoclonal antibodies would ensure the specific detection of the harmful food content for a sensitised @entity1 . METHODS: Mouse IgG antibody libraries were constructed from immunised @entity19 and specific recombinant antibodies for Mal d 1 and Api g 1 were isolated from the libraries by phage display. More detailed characterisation of the resulting antibodies was carried out using ELISA, SPR experiments and immunoprecipitation assays. RESULTS: The allergen-specific @entity1334 fragments exhibited high affinity towards the target recombinant allergens. Furthermore, the @entity1334 fragments also recognised native allergens from natural sources. Interestingly, isolated Mal d 1-specific antibody bound also to Bet v 1, the main allergen eliciting the cross-reactivity syndrome between the birch pollen and apple. Despite the similarities in Api g 1 and Bet v 1 tertiary structures, the isolated Api g 1-specific antibodies showed no cross-reactivity to Bet v 1. CONCLUSIONS: Here, high-affinity allergen-specific recombinant antibodies were isolated with interesting binding properties. With further development, these antibodies can be utilised as tools for the specific and reliable detection of allergens from different consumable products. This study gives new preliminary insights to elucidate the mechanism behind the @entity2023 and to study the IgG epitope of the allergens.
