Antinuclear antibodies in XXXX with @entity1252 : a comparison of counterimmunoelectrophoresis and immunoblotting.

@entity1 @entity1310 @entity8 @entity6016 @entity95 @entity1252 @entity1503
Traditionally, the method used mostly to identify antinuclear antibody (ANA) specificities is the counterimmunoelectrophoresis technique (CIE), in which a salt extract of @entity95 thymus powder (so-called extractable nuclear antigen or ENA) serves as the source of antigen. Recently, the immunoblotting technique (IBT) has been introduced in the serology of antinuclear antibodies. A nuclear extract of HeLa cells is generally used as antigen in this method. In this paper, we compared both methods using sera of @entity1 with active @entity1252 ( @entity1252 ). Only anti-Sm, anti-RNP, and Anti- @entity6016 were taken into consideration, as the former technique only allowed the identification of these specificities. Within these restrictions, we found that, of 77 @entity1 with @entity1252 , 21 had CIE-detectable antibodies in their circulation and 29 IBT-detectable antibodies. Anti-RNP and anti- @entity6016 were detected more frequently with the CIE than with the IBT; anti-Sm, on the other hand, was detected more frequently with the IBT than with the CIE. Several significant correlations were found between incidences of measured antibody specificities and disease features. The presence of anti-RNP (both if measured with the IBT or with the CIE) was found to be negatively correlated with @entity1310 . If measured with the IBT, the presence of anti-Sm correlated negatively with @entity1503 , and the presence of anti- @entity6016 correlated positively with @entity8 .(ABSTRACT TRUNCATED AT 250 WORDS)
