Subcellular distribution of dietary XXXX in @entity1573 liver.

@entity844 @entity2066 @entity904 @entity1474 @entity1881 @entity969 @entity1573 @entity12218
Studies were conducted examining the subcellular distribution of @entity2066 (BC), @entity1881 (E) and @entity904 (A) in livers of control and BC-fed male White Leghorn chicks. Chicks were fed Cornell B @entity1573 starter diet with or without the addition of 0.5 g BC/kg diet. A first study involved liver fractionation by differential centrifugation in 0.25 M @entity969 followed by high performance liquid chromatographic (HPLC) analyses of all fractions for quantitation of BC, E and A. A second study employed both intravenous injection of Triton @entity12218 four days prior to sacrifice and centrifugation in 1.0 M @entity969 to separate mitochondria from lysosomes more efficiently. Fraction purity was assessed by marker enzyme analyses. Results showed that @entity1573 liver accumulated BC; BC-fed chicks had higher concentrations of BC in all fractions relative to controls, and the mitochondrial fraction contained the highest concentration of BC, followed by lysosomes, microsomes and nuclei, respectively. Plasma BC increased more than fivefold in BC-fed chicks. Dietary BC increased A and E levels in liver and in the mitochondrial and lysosomal fractions while the plasma E level was decreased. Plasma A changed little with BC feeding. While dietary BC had no effect on @entity844 composition of subcellular fractions, the increase in E resulted in a large increase in the molar ratio of E to @entity1474 . The incorporation of BC and increased amounts of E into cellular membranes presumably would result in increased resistance to peroxidative damage.
