Application of the XXXX to the assessment of genetic alterations of @entity14 .

@entity124 @entity5 @entity876 @entity18432 @entity74 @entity14 @entity1412 @entity694
The @entity18432 ( @entity18432 ) allows for the isolation of pure @entity5 crypts from @entity694 tissue. In a previous study we reported on the genetic alterations found in @entity14 crypts using the @entity18432 ; however, a direct comparison of the genetic alterations found in @entity14 using either conventional methods (CM) or the @entity18432 has not previously been performed. Here, we analyzed the impact of this method on the genetic analysis of @entity694 cells by comparing the observed frequency of genetic alterations in @entity694 isolated using CM or the @entity18432 . We used a combination of the @entity18432 and the fluorescent polymerase chain reaction assay to accurately assess the incidence of @entity74 ( @entity74 ) at a number of chromosomal loci (17p, 5q, 18q, 1p, 8p, 22q), @entity124 ( @entity124 ), and mutations of @entity5 -related genes ( @entity876 and @entity1412 genes) in 48 sporadic @entity14 . In addition, genetic alterations seen in @entity5 (defined as @entity5 with both diploid and aneuploid cell populations) identified by the @entity18432 were also examined. The incidence of @entity74 at the chromosomal loci tested was more frequently detected in samples isolated from @entity5 using the @entity18432 than in those isolated from the same @entity5 using CM. In contrast, we observed no differences in the frequency of @entity124 or @entity5 -related gene mutation between the two groups. Although there was no difference in the frequency of genetic alterations between @entity5 with evidence of multiploidy, sorting of diploid and aneuploid populations allowed detection of distinct genetic changes. The crypt isolation method thus appears to be useful in that it allows purification of @entity5 cells and the accurate assessment of their genetic alterations. In addition, it may also be of benefit in clarifying the genetic profile of multiploid @entity5 cell populations.
