Strong Correlation Between Molecular Changes in XXXX and Concomitant @entity1373 .

@entity1204 @entity124 @entity5 @entity984 @entity1373 @entity5072 @entity74 @entity105
OBJECTIVE: @entity1204 ( @entity1204 ) results from the accumulation of @entity74 contributing to the progression from @entity1373 to @entity1204 . Information on these various genetic changes has been primarily derived from studying groups of either @entity1373 or @entity5 .We evaluated both @entity5072 and @entity1204 tissue obtained from the same surgical specimens for @entity984 mutations, @entity124 ( @entity124 ), and mismatch repair gene methylation, and results were correlated between the paired @entity5072 tissue and @entity1204 . The aim was to determine if molecular alterations appearing in ECs might also be present in the premalignant ( @entity5072 ) region of the @entity5 . METHODS: One hundred ninety-seven cases of @entity1204 with associated @entity1373 were evaluated. DNA samples were studied using primer sets for @entity984 gene codons 12/13 and for @entity124 utilizing the Bethesda panel. Methylation testing was performed on specimens that were microsatellite unstable using the MRC Holland SALSA MS-MLPA methylation-specific DNA detection kit. RESULTS: Forty-one (20.8%) of 197 @entity5 demonstrated a @entity984 mutation, with 35 (85.4%) of 41 accompanying @entity1373 also containing a @entity984 mutation. Forty-five @entity5 (22.8%) were microsatellite unstable, with 38 (84.4%) of 45 accompanying @entity1373 also demonstrating instability. Of the 45 microsatellite unstable @entity5 , 28 (62.2%) demonstrated methylation in both the @entity5 and the accompanying @entity1373 , whereas 9 pairs (20%) showed no methylation for either the @entity5 or @entity1373 . CONCLUSIONS: Approximately 95% of endometrial specimens demonstrated identical molecular findings regarding @entity984 mutation and microsatellite stability in the paired @entity5 and @entity5072 tissue. The same methylation pattern was found in 82.2% of the studied paired samples. Our findings strongly suggest that the molecular changes of @entity984 mutation, @entity124 , and methylation occur early in the @entity5 . We propose that endometrial biopsies revealing @entity1373 should be studied for these molecular alterations as an indicator of possible early @entity105 .
