The Therapeutic Potential of @entity1 Umbilical Mesenchymal Stem Cells From Wharton's Jelly in the Treatment of @entity35 Peritoneal Dialysis-Induced XXXX .

@entity1 @entity32 @entity14625 @entity82 @entity11999 @entity1970 @entity1463 @entity35 @entity298 @entity10879 @entity454
UNASSIGNED: : A major complication in continuous, ambulatory @entity298 in @entity1 with @entity454 who are undergoing long-term @entity298 ( @entity298 ) is peritoneal @entity82 , which can result in peritoneal structural changes and functional ultrafiltration failure. @entity1 umbilical mesenchymal stem cells (HUMSCs) in Wharton's jelly possess stem cell properties and are easily obtained and processed. This study focuses on the effects of HUMSCs on peritoneal @entity82 in in vitro and in vivo experiments. After 24-hour treatment with mixture of Dulbecco's modified Eagle's medium and @entity298 solution at a 1:3 ratio, primary @entity1 peritoneal mesothelial cells became susceptible to @entity298 -induced cell death. Such cytotoxic effects were prevented by coculturing with primary HUMSCs. In a @entity35 model, intraperitoneal injections of 20 mM @entity14625 ( @entity11999 ) in @entity298 solution for 3 weeks (the @entity298 / @entity11999 3W group) markedly induced @entity10879 formation, peritoneal thickening, and collagen accumulation. Immunohistochemical analyses indicated neoangiogenesis and significant increase in the numbers of ED-1- and a-smooth muscle actin ( @entity1970 )-positive cells in the thickened peritoneum in the @entity298 / @entity11999 3W group, suggesting that @entity298 / @entity11999 induced an inflammatory response. Furthermore, @entity298 / @entity11999 treatment for 3 weeks caused functional impairments in the peritoneal membrane. However, in comparison with the @entity298 / @entity11999 group, intraperitoneal administration of HUMSCs into the @entity35 significantly ameliorated the @entity298 / @entity11999 -induced @entity10879 formation, peritoneal @entity82 , @entity32 , neoangiogenesis, and ultrafiltration failure. After 3 weeks of transplantation, surviving HUMSCs were found in the peritoneum in the HUMSC-grafted @entity35 . Thus, xenografts of HUMSCs might provide a potential therapeutic strategy in the prevention of peritoneal @entity82 . SIGNIFICANCE: This study demonstrated that direct intraperitoneal transplantation of @entity1 umbilical mesenchymal stem cells into the @entity35 effectively prevented @entity298 / @entity14625 -induced @entity10879 formation, ultrafiltration failure, and peritoneal membrane alterations such as peritoneal thickening, @entity82 , and @entity32 . These findings provide a basis for a novel approach for therapeutic benefits in the treatment of encapsulating peritoneal @entity1463 .
