Superparamagnetic @entity1933 promotes osteogenic differentiation of XXXX adipose-derived stem cells.

@entity6037 @entity6032 @entity1933 @entity622 @entity3460 @entity35
Adult adipose tissue-derived stem cells (ADSCs) were found to hold great promise for use in bone tissue repair and regeneration. The present study aims to improve the osteogenesis of ADSCs by Superparamagnetic @entity1933 (SPIO), which is widely used in tissue imaging. In this study, adipose-derived stem cells were harvested from 4-week-old male Sprague-Dawley (SD) @entity35 . The proliferation rates of ADSCs labeling with or without SPIO were assessed by using @entity3460 assay. The osteogenic capability was examined by employing the @entity6032 activity detection kit. The mineralization of cells was determined by staining with Alizarin red S. Flow cytometry analysis was used to examine the cell apoptosis treated with or without SPIO. Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis was utilized to detect the @entity6037 , Opn, Ocn and @entity6032 genes in the cells. The results indicated that SPIO could promote @entity35 ADSCs proliferation and reduce @entity35 ADSCs apoptosis. Also, SPIO could significantly enhance the @entity6032 and @entity622 staining of ADSCs in -SPIO group and +SPIO group (P < 0.01). Furthermore, we also found that the expression of @entity6037 , Opn, Ocn and @entity6032 was significantly increased after SPIO treatment compared to the un-treated cells (P < 0.01). In conclusion, SPIO could promote the osteogenic differentiation of @entity35 adipose-derived stem cells, which would also become a great potential therapeutic tool in bone tissue engineering.
