miRNA-regulated delivery of @entity72489 suppresses b-catenin signaling and tumorigenicity of XXXX stem cells.

@entity5 @entity19 @entity771 @entity72489 @entity5207 @entity14
UNASSIGNED: Cancer stem cells (CSCs) are key cellular targets for effective @entity5 therapy, due to their critical roles in @entity5 progression and chemo/radio-resistance. Emerging evidence demonstrates that long non-coding RNAs (lncRNAs) are important players in the biology of @entity5 . However, it remains unknown whether lncRNAs could be exploited to target CSCs. We report that large intergenic non-coding RNA @entity5207 ( @entity72489 ) is a potent suppressor of stem-like traits of CSCs purified from both primary @entity14 ( @entity14 ) tissues and cell lines. A novel @entity72489 -expressing adenoviral vector, which was armed with miRNA responsive element (MRE) of @entity771 (Ad-lnc- @entity5207 -MRE), was generated to eliminate @entity14 CSCs. Integration of @entity771 MREs into the adenovirus efficiently delivered @entity72489 into CSCs that contained low levels of @entity771 . Moreover, @entity72489 inhibited the activity of b-catenin signaling, thereby attenuating the viability, self-renewal, and glycolysis of CSCs in vitro. By limiting dilution and serial @entity5 formation assay, we demonstrated that Ad-lnc- @entity5207 -MRE significantly suppressed the self-renewal potential and tumorigenicity of CSCs in @entity19 . Importantly, application of @entity771 MREs appeared to protect normal liver cells from off-target expression of @entity72489 in both @entity5 -bearing and na  ve @entity19 . Taken together, these findings suggest that lncRNAs may be promising therapeutic molecules to eradicate CSCs and @entity5 -suppressor miRNAs, such as @entity771 , may be exploited to ensure the specificity of CSC-targeting strategies.
