Effects of Portulaca oleracea L. Polysaccharides on Phenotypic and Functional Maturation of XXXX Bone Marrow Derived Dendritic Cells.

@entity39 @entity5 @entity8271 @entity19 @entity1618 @entity11787 @entity19711 @entity1990
Portulaca oleracea L. is an annual plant widely distributed from the temperate to the tropical zones. POL-P3b, a polysaccharide fraction purified from Portulaca oleracea L., is able to enhance immunity and inhibit @entity5 formation. Induction of antitumor immunity by @entity5 fusion cells can be modulated by their activation status. Mature dendritic cells are significantly better than immature dendritic cells at cytotoxic T-lymphocyte induction. In this study, we analyzed the effects of POL-P3b on the maturation and function of @entity19 bone-marrow-derived dendritic cells (DCs) and relevant mechanisms. The phenotypic maturation of DCs was confirmed by flow cytometry. We found that POL-P3b upregulated the expression of @entity8271 , @entity11787 , @entity19711 , and major histocompatibility complex class II molecules on DCs, stimulated production of more interleukin (IL)-12, @entity5 @entity39 factor-a, and less @entity1618 . Also, DCs pulsed POL-P3b and freeze-thaw antigen increased DCs-driven T cells' proliferation and promoted U14 cells' apoptosis. Furthermore, the expression of @entity1990 was significantly increased on DCs treated by POL-P3b. These results suggested that POL-P3b may induce DCs maturation through @entity1990 . Taken together, our results may have important implications for the molecular mechanisms of immunopotentiation of POL-P3b, and provide direct evidence to suggest that POL-P3b should be considered as a potent adjuvant nutrient supplement for DC-based vaccines.
