Detection of PHLPP1a/b in XXXX and Mouse Brain by Different Anti-PHLPP1 Antibodies.

@entity1 @entity563 @entity368 @entity71322 @entity1889
Pleckstrin homology domain and @entity368 rich repeat protein phosphatase 1 ( @entity71322 ) is a member of the @entity563 / @entity1889 family of phosphatases. It has been studied in organs including brain, heart, pancreas, adipose, breast, and prostate. @entity1 @entity71322 encodes two splice variants - PHLPP1a (~140-150   kDa) and PHLPP1b (~180-190   kDa). Commercial antibodies are widely used to characterize @entity71322 proteins in cells/tissues. Here we validate five different antibodies to detect PHLPP1a/b by Western blot using @entity71322 WT/KO mice. All antibodies recognize PHLPP1b in brain. Only a single antibody (Cosmo Bio Co) detects PHLPP1a (~145-150   kDa). The other four antibodies detect a non-specific signal at ~150   kDa as evidenced by its abundance in @entity71322 KO tissues. Results suggest Cosmo antibody is a better reagent to detect @entity71322 a by Western blot. In contrast, we found it unsuitable for immunofluorescence applications in brain. Our findings caution interpretation of the ~150   kDa band detected by some @entity71322 antibodies in rodent and @entity1 tissues. Results also recapitulate the importance of including molecular weight standards in Western blot data to simplify retrospective analysis.
