New reaction of XXXX with @entity4478 at high local concentrations. Primitive enzyme models.

@entity4315 @entity28740 @entity3265 @entity3878 @entity6115 @entity4478 @entity28196 @entity27148 @entity8776
The fluorogenic @entity6115 reagent @entity28196 (CNBFz) reacts with @entity4478 to give one of two products, or a mixture, depending on the concentration of @entity6115 . At low concentrations (e.g. 5 X 10(-4) M) of unprotonated @entity6115 the normal replacement of the @entity3878 occurs, giving lambdamax approximately 470 nm. At high concentration of unprotonated @entity6115 (e.g. 0.5 M), a new product, lambdamax approximately 380 nm, is produced. At intermediate concentrations, both products are seen. These results were obtained with aqueous @entity3265 , @entity8776 and @entity28740 , and with @entity28740 in dry @entity4315 . The 380 nm product also is obtained with high local concentrations of @entity4478 , such as with [lys]n at pH 8.5-10 and micellar @entity27148 at pH 9.5. These results are suggested to be analogous to those of very primitive proto-enzyme systems, by which clusters of small molecules can produce quantitative changes in "metabolism". Reaction of CNBFz with gelatin gave qualitative evidence for cross-linking.
