Possibility of false-positive detection for sporozoites in mosquitos (Diptera: Culicidae) by nested polymerase chain reaction using XXXX genomic DNA.

@entity1 @entity19 @entity1503 @entity197 @entity501 @entity311
Anopheles stephensi Liston and An. saperoi Bohart and Ingram infected with the rodent @entity311 parasite @entity501 nigeriense. They were examined 12 and 19 days after blood feeding for sporozoites in head with anterior thorax (HT) and oocysts in abdomen with posterior thorax (AB) by light microscopy and by the nested polymerase chain reaction (nested PCR-based on the amplification of the sequences of the small subunit ribosomal RNA gene). The detection rate of parasite DNA by nested PCR in HT samples 12 days after blood feeding was similar to that by microscopic method. However, in HT samples 19 days after blood feeding, the rate by the PCR method was higher than that by the microscopic method. The incidence of sporozoites in salivary glands of infected mosquitos for 12 days after blood sucking was examined by the PCR method. Parasite DNA in HT of @entity197 Skuse (a non vector for the rodent @entity311 ) as well as An. stephensi and An. saperoi was detected for up to 4 days after feeding on @entity19 with the rodent @entity311 parasites. The results indicate that when the PCR method is used for detection of sporozoites of @entity1 @entity311 in mosquitos collected in the field, there are possibilities of including false-positive data for mosquitos that have just or recently fed on @entity1 @entity1503 with @entity311 (erythrocytic form).
