Identification of Pathogen Signatures in XXXX Using RNA-seq.

@entity1 @entity97 @entity263 @entity8999 @entity19 @entity5 @entity2348 @entity5478 @entity1778
UNASSIGNED: Infections of the prostate by bacteria, @entity1 papillomaviruses, polyomaviruses, xenotropic @entity19 @entity1778 virus (MLV)-related gammaretroviruses, @entity1 cytomegaloviruses and other members of the herpesvirus family have been widely researched. However, many studies have yielded conflicting and controversial results. In this study, we systematically investigated the transcriptomes of @entity1 prostate samples for the unique genomic signatures of these pathogens using RNA-seq data from both western and Chinese @entity1 . @entity1 and nonhuman RNA-seq reads were mapped onto @entity1 and pathogen reference genomes respectively using alignment tools Bowtie and BLAT. Pathogen infections and integrations were analyzed in adherence with the standards from published studies. Among the nine pathogens ( @entity5478 , HPV, HCMV, XMRV, @entity8999 , JCV, SV40, EBV, and @entity97 ) we analyzed, @entity5478 genes were detected in all @entity263 samples and all adjacent samples, but not in prostate samples from healthy individuals. SV40, HCMV, EBV and low-risk HPVs transcripts were detected in one @entity5 sample and two adjacent samples from @entity263 @entity1 , but not in any samples of @entity263 @entity1 ; XMRV, @entity8999 and JCV sequences were not identified in our work; @entity97 , as a negative control, was absent from any samples. Moreover, no pathogen integration was identified in our study. While further validation is required, our analysis provides evidence of @entity5478 infections in @entity1 @entity263 . Noted differences in viral infections across ethnicity remain to be confirmed with other @entity263 data sets. The effects of @entity2348 and their contributions to @entity263 pathogenesis will require continuous research on associated pathogens.
