Curcumin attenuates quinocetone-induced oxidative stress and genotoxicity in XXXX hepatocyte L02 cells.

@entity1 @entity137 @entity1367
UNASSIGNED: Quinocetone (QCT), a new quinoxaline 1,4-dioxides, has been used as antimicrobial feed additive in China. Potential genotoxicity of QCT was concerned as a public health problem. This study aimed to investigate the protective effect of curcumin on QCT-induced oxidative stress and genotoxicity in @entity1 hepatocyte L02 cells. Cell viability and intracellular reactive oxygen species (ROS), biomarkers of oxidative stress including @entity1367 ( @entity1367 ) activity and glutathione (GSH) level were measured. Meanwhile, comet assay and micronucleus assay were carried out to evaluate genotoxicity. The results showed that, compared to the control group, QCT at the concentration ranges of 2-16    g/mL significantly decreased L02 cell viability, which was significantly attenuated with curcumin pretreatment (2.5 and 5    M). In addition, QCT significantly increased cell oxidative stress, characterized by increases of intracellular ROS level, while decreased endogenous antioxidant biomarkers GSH level and @entity1367 activity (all p   <   0.05 or 0.01). Curcumin pretreatment significantly attenuated ROS formation, inhibited the decreases of @entity1367 activity and GSH level. Furthermore, curcumin significantly reduced QCT-induced DNA fragments and micronuclei formation. These data suggest that curcumin could attenuate QCT-induced @entity137 and genotoxicity in L02 cells, which may be attributed to ROS scavenging and anti-oxidative ability of curcumin. Importantly, consumption of curcumin may be a plausible way to prevent quinoxaline 1,4-dioxides-mediated oxidative stress and genotoxicity in @entity1 or animals.
