Assessment of an Orofacial Operant XXXX Assay as a Preclinical Tool for Evaluating Analgesic Efficacy in Rodents.

@entity2951 @entity19 @entity58260 @entity4783 @entity158 @entity954 @entity35 @entity130
A model system capable of providing clinically relevant analgesic doses with minimal @entity130 has been elusive in laboratory animal medicine. Our laboratory has developed an @entity158 system that effectively discriminates between nonnoxious and noxious thermal stimuli in @entity35 and @entity19 . Male and female @entity35 (Crl:SD) and @entity19 (Crl: @entity58260 -HR(hr)) were trained to perform a task (placing their face through an opening and having their cheeks stay in contact with thermodes) to receive a reward (a solution of sweetened condensed milk). Currently accepted doses of @entity4783 were tested by using a crossover design. @entity158 was induced in both species by sensitizing the depilated skin over both cheeks with @entity2951 cream or by creating a @entity954 ( @entity35 only) and then allowing the animals to contact a temperature-regulated thermode while obtaining a reward. Optimal antinociceptive doses included 0.05 and 0.1 mg/kg in male @entity19 but only 0.05 mg/kg in female @entity19 . In rats, optimal antinociceptive doses included 0.03 and 0.05 mg/kg for male @entity35 but only 0.03 mg/kg for female @entity35 . The 2 pain-induction models in @entity35 ( @entity2951 cream and @entity954 ) did not differ. Our @entity158 assay can determine clinically relevant analgesic doses for rodents in a preclinical assay. The automated, investigator-independent nature of the assay, in conjunction with its high sensitivity, makes this method an improvement over traditional noninvasive methods, providing better data for developing optimal analgesic recommendations for @entity35 and @entity19 .
