Neutrophil @entity5689 expression contributes to mechanism of XXXX and injury.

@entity5038 @entity55 @entity19 @entity5037 @entity651 @entity5689 @entity5180 @entity1317 @entity224 @entity3895
@entity5689 present in immune cells may be involved in regulation of the inflammatory response. Here, using @entity5689 -null ( @entity3895 -1(-/-)) @entity19 , we addressed the role of @entity5689 in polymorphonuclear neutrophils (PMNs) in regulating PMN activation-mediated @entity651 . In lungs of wild-type ( @entity3895 -1(+/+)) @entity19 perfused at constant flow with Krebs-Henseleit solution, addition of @entity3895 -1(+/+) PMNs (4 x 10(6) cells) into the perfusate followed by their activation with @entity5037 ( @entity5038 , 1.0 muM) plus platelet-activating factor (1.0 nM) increased pulmonary microvessel filtration coefficient by 150% and wet-to-dry lung weight ratio by 50% as well as PMN accumulation in lungs. These responses were markedly reduced in lungs perfused with @entity3895 -1(-/-) PMNs followed by addition of the same activating agents. @entity5038 -stimulated adhesion of @entity3895 -1(-/-) PMNs to pulmonary microvascular endothelial cells and migration of @entity3895 -1(-/-) PMNs across endothelial monolayers were also impaired compared with @entity3895 -1(+/+) PMNs. @entity3895 -1(-/-) PMNs showed 50-80% reduction in PMA- or @entity5038 -stimulated @entity224 production compared with @entity3895 -1(+/+) PMNs. In addition, @entity3895 -1(-/-) PMNs had decreased migratory activity (50%) and adhesion to fibrinogen (40%) in response to @entity5038 . @entity5180 and Rac2 were activated in @entity3895 -1(+/+) PMNs after stimulation of @entity5038 but not in @entity3895 -1(-/-) PMNs. Exogenous expression of @entity5689 in COS-phox cells augmented the @entity5038 -induced @entity5180 activation and @entity224 production, indicating a direct role of @entity5689 in the mechanism of @entity224 production. Thus @entity5689 expression in PMNs plays a key role in mediating PMN activation, adhesion, and transendothelial migration and in PMN activation-induced @entity55 and @entity1317 .
