Nasal carriage of coagulase positive staphylococci in XXXX of a Primary-Healthcare-Center: genetic lineages and resistance and virulence genes.

@entity1 @entity799 @entity5176 @entity114 @entity794 @entity43490 @entity7187 @entity23819 @entity803 @entity35995
INTRODUCTION: @entity794 and @entity23819 are highly important due to their capacity for producing diseases in @entity1 and animals, respectively. The aim of the study was to investigate and characterize the coagulase positive @entity794 (CoPS) carriage in a Primary Healthcare Center population. METHODS: Nasal swabs were obtained from 281 non-infectious @entity1 . The CoPS isolates recovered were typed, and their resistance phenotype and genotype, as well as their virulence profiles, were analyzed. RESULTS: CoPS isolates were recovered from 56/281 @entity1 (19.9%). Fifty-five were @entity794 (19.6%), 54 were methicillin susceptible (MSSA) and one was methicillin resistant ( @entity794 ). The remaining isolate was @entity23819 (0.4%). A high diversity of spa-types (n=40) was detected, with 6 of them being new ones. The multi-locus-sequence-typing of 13 MSSA and one @entity794 selected isolates was performed and the STs detected were: ST8, ST15, ST30, ST34, ST121, ST146, ST398, ST554, ST942, ST2499, and ST2500 (the last two STs being new). One MSSA isolate was typed as t1197-ST398-(Clonal complex)CC398. The @entity794 isolate was typed as t002-ST146-CC5-SCCmec-IVc, and exhibited a multiresistance phenotype. The detected resistances were: @entity114 (76%), @entity7187 (7%), @entity803 (7%), @entity799 (7%), @entity5176 (7%), and @entity35995 (5%). Five isolates contained lukF/lukS-PV genes, 17 tst gene, one eta gene, and two @entity43490 gene. The @entity23819 isolate presented a new spa-type (t57) (belonging to a new ST180) and the genes lukS/F-I, siet, se-int, and expB. CONCLUSIONS: A high genetic diversity of @entity794 was detected. Mention must be made of the identification of MSSA CC398 and @entity23819 isolates in two @entity1 , one of them with animal contact. The detection of the genes lukF/lukS-PV and tst should be noted.
