Effect of estrogen on the expression of a cell-surface antigen associated with XXXX anterior pituitary somatotrophs.

@entity5 @entity4743 @entity977 @entity35 @entity4742
The long-term in vivo effect of @entity4742 ( @entity4743 ) on the expression of a cell-surface antigen associated with the anterior pituitary somatotroph was studied in two strains of female @entity35 using double immunofluorescence techniques. Mab WHC-1, a recently generated and characterized monoclonal antibody, was used to detect the antigen associated with somatotrophs, whereas rabbit anti-rat prolactin (rPRL) and anti-human growth hormone (hGH) antisera were used to identify mammotrophs and somatotrophs, respectively. In F344 @entity35 , Mab WHC-1-positive cells increased from 13.8 +/- 0.5% of total pituitary cells in normal anterior pituitaries to 34.2 +/- 4.0% in @entity4743 -induced @entity977 . The number of mammotrophs also increased significantly from 58.0 +/- 3.2% in controls to 75.9 +/- 2.2% in @entity5 . On the other hand, somatotrophs decreased significantly in number following ovariectomy (OVX) and @entity4743 implantation (19.7 +/- 0.5% vs. 6.1 +/- 1.2%). Based on double immunofluorescence, the percentage of Mab WHC-1-positive cells, which were somatotrophs, decreased from 85.5 +/- 2.7% in normal controls to 6.7 +/- 1.5% in @entity4743 -induced @entity5 . On the other hand, the percentage of Mab WHC-1-positive cells which were mammotrophs increased significantly from 14.0 +/- 1.4% to 86.1 +/- 1.8% following OVX and @entity4743 implantation. A similar change was found in the number of somatotrophs and mammotrophs following the same treatment in Sprague-Dawley (SD) @entity35 which did not develop @entity977 . In contrast to F344 @entity35 , the number of Mab WHC-1-positive cells in SD @entity35 decreased significantly from 32.4 +/- 2.8% in sham-operated controls to 19.3 +/- 2.9% in OVX + @entity4743 -implanted @entity35 .(ABSTRACT TRUNCATED AT 250 WORDS)
