Simultaneous determination of ten antiepileptic drugs in XXXX plasma by liquid chromatography and tandem mass spectrometry with positive/negative ion-switching electrospray ionization and its application in therapeutic drug monitoring.

@entity1 @entity3933 @entity2812 @entity4677 @entity2051 @entity1092 @entity1626 @entity15059 @entity1771 @entity3174 @entity109 @entity15058 @entity302 @entity2049
UNASSIGNED: A simple, rapid and high-throughput liquid chromatography with tandem mass spectrometry method for the simultaneous quantitation of ten antiepileptic drugs in @entity1 plasma has been developed and validated. The method required only 10  L of plasma. After simple protein precipitation using @entity1626 , the analytes and internal standard @entity3933 were separated on a Zorbax SB-C18 column (50 * 4.6 mm, 2.7  m) using @entity1626 /water as the mobile phase at a flow rate of 0.9 mL/min. The total run time was 6 min for each sample. The validation results of specificity, matrix effects, recovery, linearity, precision and accuracy were satisfactory. The lower limit of quantification was 0.04  g/mL for @entity2049 , 0.02  g/mL for @entity2812 , 0.01  g/mL for @entity2051 , 0.4  g/mL for @entity15059 , 0.1  g/mL for @entity15058 , 0.15  g/mL for @entity4677 , 0.06  g/mL for @entity3174 , 0.3  g/mL for @entity1092 , 0.03  g/mL for @entity1771 , and 0.15  g/mL for @entity109 . The intraday precision and interday precision were less than 7.6%, with the accuracy ranging between -8.1 and 7.9%. The method was successfully applied to therapeutic drug monitoring of 1237 @entity1 with @entity302 after administration of standard antiepileptic drugs. The method has been proved to meet the high-throughput requirements in therapeutic drug monitoring. This article is protected by copyright. All rights reserved.
