Is transplantation of a few leukemic cells inside an artificial ovary able to induce XXXX in an experimental model?

@entity1 @entity5 @entity19 @entity40261 @entity262 @entity915 @entity1778 @entity465
PURPOSE: To evaluate the @entity5 -inducing ability of a few leukemic cells xenotransplanted inside an artificial ovary. METHODS: Ten and 100 @entity1 leukemic cells were embedded in a fibrin matrix along with 50,000 @entity1 ovarian stromal cells, and grafted to the peritoneal bursa of 5 and 5 @entity915 @entity19 respectively. Four @entity19 grafted with 3x10(6) leukemic cells in fibrin served as positive controls. At 20 weeks post-transplantation, the grafts, liver, spleen, blood and bone marrow were analyzed for the presence of @entity1778 by anti- @entity40261 IHC, flow cytometry (FC) and PCR. RESULTS: All @entity19 grafted with 3x10(6) cells developed peritoneal masses 4 weeks after xenotransplantation, and @entity262 was confirmed by IHC, PCR and FC. Among @entity19 grafted with 10 or 100 leukemic cells, none showed any sign of @entity1778 after 20 weeks, and IHC, FC and PCR on the different recovered tissues all proved negative. CONCLUSION: This study investigates the @entity5 -inducing potential of a few leukemic cells grafted inside an artificial ovary. Transplantation of 100 leukemic cells appears to be insufficient to induce @entity1778 after 20 weeks. These results in an @entity465 xenografting model are quite reassuring. However, for clinical application, follicle suspensions must be purged of leukemic cells before grafting, as even the slightest risk should be avoided.
