Field evaluation of a rapid diagnostic test to detect antibodies in XXXX @entity13871 .

@entity1 @entity9157 @entity1170 @entity5603 @entity281 @entity13871
UNASSIGNED: @entity1 @entity13871 which is caused mainly by the larvae of @entity5603 and @entity9157 , is a worldwide zoonotic disease that can be a potentially serious @entity1 @entity281 . The enzyme-linked immunosorbent assay (ELISA) using @entity5603 excretory-secretory (TES) antigens harvested from @entity5603 larvae is currently the serological test for confirming @entity13871 . An alternative to producing large amounts of Toxocara TES and improved diagnosis for @entity13871 is through the development of highly specific recombinant antigens such as the @entity5603 second stage larva excretory-secretory 30kDa protein (recTES-30). The aim of this study was to evaluate the sensitivity and specificity of a @entity1170 ( @entity1170 , named as iToxocara kit) in comparison to recTES-30 ELISA in Serendah Orang Asli village in Selangor, Malaysia. A total of 133 subjects were included in the study. The overall prevalence rates by ELISA and @entity1170 were 29.3% and 33.1%, respectively, with more positive cases detected in males than females. However, no association was found between @entity13871 and gender or age. The percentage sensitivity, specificity, positive predictive value and negative predictive value of @entity1170 were 85.7%, 90.1%, 80% and 93.2%, respectively. The prevalence for @entity13871 in this population using both ELISA and @entity1170 was 27.1% (36/133) and the K-concordance test suggested good agreement of the two tests with a Cohen's kappa of 0.722, P<0.01. In addition, the followed-up Spearman rank correlation showed a moderately high correlation at R=0.704 and P<0.01. In conclusion, the @entity1170 kit was faster and easier to use than an ELISA and is useful for the laboratory diagnosis of hospitalized cases of @entity13871 .
