The mare as a model for luteinized XXXX : intrafollicular endocrine milieu.

@entity1 @entity12567 @entity1641 @entity1420 @entity7 @entity1421 @entity3206 @entity409 @entity22667 @entity2918 @entity35120 @entity46 @entity701 @entity230 @entity33422 @entity3258
UNASSIGNED: Luteinized unruptured follicle (LUF) syndrome is a recurrent @entity409 that affects up to 23% of @entity1 with normal menstrual cycles and up to 73% with @entity46 . Mechanisms underlying the development of @entity33422 in mares were studied to provide a potential model for @entity1 @entity3206 . The effect of extended increase in circulating @entity35120 achieved by administration of recombinant @entity1641 @entity35120 (reLH) or a short surge of @entity35120 and decrease in @entity1420 induced by @entity22667 ( @entity22667 ) on LUF formation (Experiment 1), identification of an optimal dose of @entity12567 inhibitor ( @entity3258 , FM; to block the effect of @entity2918 ) for inducing LUFs (Experiment 2), and evaluation of intrafollicular endocrine milieu in LUFs (Experiment 3) were investigated. In Experiment 1, mares were treated with reLH from Days 7-15 (Day 0 = ovulation), @entity22667 on Day 7, or in combination. In Experiment 2, FM at doses of 2.0 or 3.0 mg/kg every 12 h and hCG (1500 IU) were administered after a follicle >= 32 mm was detected. In Experiment 3, FM at a dose of 2.0 mg/kg every 12 h plus hCG was used to induce LUFs and investigate the intrafollicular endocrine milieu. No LUFs were induced by reLH or @entity22667 treatment; however, LUFs were induced in 100% of mares using FM. Intrafollicular @entity22667 metabolite (PGFM), @entity22667 , and @entity1421 were lower and the ratio of @entity1421 : @entity22667 was higher in the Induced LUF group. Higher levels of intrafollicular E2 and total primary sex @entity230 were observed in the Induced LUF group along with a tendency for higher levels of GH, @entity701 , and T; however, @entity35120 , PRL, @entity7 , and NO did not differ between groups. In conclusion, this study reveals part of the intrafollicular endocrine milieu and the association of @entity2918 in LUF formation, and indicates that the mare might be an appropriate model for studying the poorly understood @entity33422 .
