XXXX inhibits @entity14489 channels in HEK293 cells.

@entity3091 @entity3096 @entity17054 @entity14489
UNASSIGNED: @entity3091 ( @entity17054 ) is a @entity3096 that exerts anti  -arrhythmic effects. However, its potential effects on ion channels have remained elusive. The aim of the present study was to investigate the effects of @entity17054 on @entity14489  channels in HEK293  cells. The current of @entity14489 channels (Ikur) in HEK293  cells was recorded using the whole  -cell patch  -clamp technique and the expression of the @entity14489 protein was measured using western blot analysis 24  h after treatment with @entity17054 . The results showed that 5    M @entity17054 significantly reduced Ikur from 215.04  40.59 to 77.72  17.94  pA/pF (P<0.05; n=5). @entity17054 increased the current suppression from 0 to 0.31  0.12 and 0.55  0.11 over 5 or 20  min, respectively. In addition, Ikur decreased from 376.23  1.30 to 270.19  4.28  pA/pF when the frequency was increased from 0.5 to 4  Hz in HEK293  cells treated with 10    M @entity17054 for 5  min. Furthermore, @entity17054 reduced @entity14489 protein expression in a dose  -dependent manner. These results demonstrated that @entity17054 inhibited Ikur and the expression of @entity14489 in HEK293  cells in a dose  -, time  - and frequency  -dependent manner. These observations partly explained the mechanisms by which @entity17054 exerts anti  -arrhythmic effect.
