High-performance liquid chromatographic analysis of new XXXX antifungal agent @entity72743 and its derivatives in plasma.

@entity35 @entity72743 @entity10998 @entity1626
A simple reversed-phase high-performance liquid chromatography (HPLC) method with UV detection was developed and validated for the quantitation of @entity72743 , a novel @entity10998 antifungal agent and its analogs in @entity35 plasma. The method involved a simple precipitation of plasma protein with @entity1626 (1:10 ratio). The reconstituted sample after evaporation to dryness was injected onto a HPLC column. @entity72743 and its analogs were separated from the matrix components on a symmetry C18 column using an aqueous mobile phase of @entity1626 and water with a flow rate of 1 ml min(-1). A step gradient of 40-80% @entity1626 eluted all four compounds. The run time was 30 min. The linear range was 0.5 10 microg ml(-1)(r2 > 0.999). The limit of quantitation was 0.5 microg ml(-1). The inter-day precision and accuracy for @entity72743 standard concentration were from 1.9 to 8.5% and from 1.4 to +/- 4.40%, respectively. The precision and accuracy of intra-day quality control samples were from 4.6 to 5.2% and from 4.6 to 12%, respectively.
