Role of @entity9094 and @entity14570 in a model of chronic allergen exposure: a XXXX comparison study.

@entity32 @entity641 @entity19 @entity565 @entity409 @entity9094 @entity14570 @entity58 @entity1312 @entity1224
BACKGROUND: Asthma is a disease characterized by variable and reversible @entity1224 and is associated with airway @entity32 , airway remodelling (including goblet cell hyperplasia, increased collagen deposition and increased smooth muscle mass) and increased airway responsiveness. It is believed that airway @entity32 plays a critical role in the development of airway remodelling, with @entity1312 and @entity641 pathways being strongly associated with the disease progression. @entity19 models of @entity565 are capable of recapitulating some components of @entity565 and have been used to look at both @entity1312 and @entity641 pathways, which use @entity9094 and @entity14570 signalling molecules, respectively. OBJECTIVES: Using brief and chronic models of allergen exposure, we utilized BALB/c and C57Bl/6 to explore the hypothesis that observed differences in responses to allergen between these @entity19 strains will involve fundamental differences in @entity1312 and @entity641 responses. METHODS: The following outcome measurements were performed: airway physiology, bronchoalveolar lavage cell counts/cytokine analysis, histology, immunoblots and gene expression assays. RESULTS: We demonstrate in BALB/c @entity19 an @entity1312 -dependent phosphorylation of @entity9094 , nuclear localized in inflammatory cells, which is associated with indices of airway remodelling and development of @entity409 . In BALB/c @entity19 , phosphorylation of @entity14570 is delayed relative to @entity9094 activation and also involves an @entity1312 -dependent mechanism. In contrast, despite an allergen-induced increase in @entity58 , @entity1312 and eosinophils, C57Bl/6 demonstrates a reduced and distinct pattern of phosphorylated @entity9094 , no @entity14570 phosphorylation changes and fail to develop indices of remodelling or changes in airway function. CONCLUSION: The activation of signalling pathways and nuclear translocation of signalling molecules downstream of @entity1312 and @entity641 further support the central role of these molecules in the pathology and dysfunction in animal models of @entity565 . Activation of signalling pathways downstream from @entity1312 and @entity641 may be more relevant in disease progression than elevations in airway @entity32 alone.
