Proteome, phosphoproteome, and hydroxyproteome of liver mitochondria in XXXX @entity35 at early pathogenic stages.

@entity2175 @entity308 @entity6 @entity26 @entity6626 @entity35 @entity413 @entity2383
It has been proposed that @entity2383 is involved in the pathogenesis of @entity2175 ( @entity2175 ). To dissect the underlying mechanisms, we performed a multiplexed proteomics study on liver mitochondria isolated from a spontaneous @entity6 @entity35 model before/after they were rendered @entity6 . Altogether, we identified 1091 mitochondrial proteins, 228 phosphoproteins, and 355 hydroxyproteins. Mitochondrial proteins were found to undergo expression changes in a highly correlated fashion during @entity2175 development. For example, proteins involved in beta-oxidation, the @entity6626 cycle, oxidative phosphorylation, and other bioenergetic processes were coordinately up-regulated, indicating that liver cells confronted @entity2175 by increasing energy expenditure and activating pathways that rid themselves of the constitutively increased flux of @entity413 and lipid. Notably, activation of oxidative phosphorylation was immediately related to the overproduction of reactive @entity26 species, which caused oxidative stress within the cells. Increased oxidative stress was also evidenced by our post-translational modification profiles such that mitochondrial proteins were more heavily hydroxylated during @entity2175 development. Moreover, we observed a distinct @entity308 of antiapoptosis and antioxidative stress proteins that might reflect a higher apoptotic index under the @entity6 stage. We suggest that such changes in systematic metabolism were causally linked to the development of @entity2175 . Comparing proteomics data against microarray data, we demonstrated that many @entity2175 -related alterations were unidentifiable by either proteomics or genomics approaches alone, underscoring the importance of integrating different approaches. Our compendium could help to unveil pathogenic events in mitochondria leading to @entity2175 and be useful for the discovery of diagnosis biomarker and therapeutic targets of @entity2175 .
