Tissue plasminogen activator coating on surface of implants reduces XXXX biofilm formation.

@entity19 @entity794 @entity5152 @entity2348 @entity35311 @entity281
BACKGROUND: @entity794 ( @entity794 ) biofilm infections of indwelling medical devices are a major medical challenge due to their high prevalence and antibiotic resistance. As fibrin plays an important role in @entity794 biofilm formation, we hypothesize that coating with fibrinolytic agents on the surface of implants can be used as a new anti-biofilm prophylaxis. METHODS: The effect of tissue plasminogen activator ( @entity35311 ) coating on @entity794 biofilm formation was tested with in vitro microplate biofilm assays and an in vivo @entity19 model of @entity281 . RESULTS: @entity35311 coating efficiently inhibited biofilm formation by various @entity794 strains. The effect was dependent on plasminogen activation by @entity35311 , leading to subsequent local fibrin cleavage. @entity35311 coating on implant surfaces prevented both the early adhesion and the later biomass accumulation. Furthermore, @entity35311 coating increased susceptibility of biofilm infections to antibiotics. In vivo, significantly less bacteria were detected on the surface of implants coated with @entity35311 compared to control implants from @entity19 treated with @entity5152 . CONCLUSIONS: Fibrinolytic coatings (e.g. with @entity35311 ) reduce @entity794 biofilm formation both in vitro and in vivo, suggesting a novel concept to prevent @entity2348 on indwelling medical devices.
