In situ hybridization analysis of the expression of XXXX telomerase RNA in normal and pathologic conditions of the skin.

@entity1 @entity1569 @entity5 @entity1489 @entity957 @entity5526 @entity28373 @entity320 @entity1491
@entity1 telomerase RNA ( @entity28373 ) expression in skin was examined by in situ hybridization analysis. All newborn foreskins examined (n = 5) expressed @entity28373 in epidermal basal cells at moderate levels. Telomerase RNA was not detectable in most adult specimens from sun protected areas (six of seven), whereas all samples obtained from sun exposed areas (n = 8) showed moderate @entity28373 signals in epidermal basal cells. Telomerase RNA was also detected at moderate to strong levels in basal cells of @entity1569 , @entity5526 , and the proliferative cells of the anagen hair bulb. @entity1491 samples (14 of 15) had moderate to high @entity28373 expression throughout the entire @entity5 , whereas @entity957 (seven of eight) showed variable intensities of @entity28373 expression but only in the cells at the periphery of @entity5 nests. All @entity320 examined (n = 5) had moderate @entity28373 expression in all @entity5 cells. The @entity28373 signal intensities in @entity1489 did not correlate with the age or sex of the donors, the clinical history of the lesions, or the histologic subtypes. To address whether @entity28373 expression correlated with the proliferative state, sequential sections were stained with anti-Ki-67 antibody, a proliferation marker. In newborn foreskins, @entity957 , and @entity1491 , the distributions of @entity28373 and Ki-67 were similar but not always identical. Telomerase RNA was more abundant than Ki-67 in the basal and suprabasal layer of newborn foreskins, suggesting that @entity28373 expression is present both in actively cycling and in resting cells.
