Identical MicroRNAs Regulate Liver Protection during Anaesthetic and Ischemic Preconditioning in XXXX : An animal study.

@entity5721 @entity9096 @entity2036 @entity63 @entity409 @entity5906 @entity12003 @entity3929 @entity2614 @entity35 @entity99
UNASSIGNED: Anaesthetic preconditioning (APC) and @entity63 preconditioning (IPC) ameliorate @entity409 -reperfusion (I/R) injury and are important for regulating @entity5721 . MicroRNAs (miRNAs) are short, noncoding RNA molecules of 21-23 @entity2036 in length, and are currently under intensive investigation regarding their ability to regulate gene expression in a wide range of species. miRNA activity is involved in controlling a wide range of biological functions and processes. We evaluated whether APC and IPC are mediated by the same miRNAs by performing comprehensive miRNA screening experiments in a @entity35 model of @entity5721 . Twenty-one @entity35 were randomly divided into three groups (n = 7/group): control (mock preconditioning), APC, and IPC. Control @entity35 were subjected to 60 min of @entity99 followed by 4 h of reperfusion, whereas the APC and IPC groups were preconditioned with 2% @entity2614 and @entity99 for 10 min prior to @entity63 -reperfusion, respectively. Liver samples were collected to measure miRNA levels after 3 h of reperfusion, and gene networks and canonical pathways were identified using Ingenuity Pathway Analysis (IPA). Blood samples were collected to measure the levels of @entity5906 aminotransferase (AST) and @entity3929 aminotransferase (ALT). Although haemodynamic parameters did not vary among the groups, AST and ALT levels were significantly higher in the control group than in the APC and IPC groups. Comprehensive miRNA screening experiments revealed that most miRNAs altered in the APC group were common to those in the IPC group. IPA identified five miRNAs related to the Akt-glycogen synthase kinase-3b ( @entity9096 )- @entity12003 pathway that were significantly affected by both preconditioning strategies. The application of either APC or IPC to ameliorate @entity5721 results in expression of several common miRNAs that are related to the Akt-GSK- @entity12003 pathway.
