Expression of the gangliosides GD3 and GD2 on lymphocytes in tissue sections of XXXX .

@entity14327 @entity14328 @entity1573 @entity1358 @entity3 @entity320
Previous studies in vitro have shown that monoclonal antibodies (MAbs) against gangliosides GD3 and GD2 potentiate lymphocyte responses to a variety of stimuli. The purpose of the present study was to determine by immunohistological techniques whether GD3 and GD2 was expressed on lymphoid cells in vivo around @entity320 cells. Studies on @entity3 in lymph nodes indicated that the lymphoid infiltrate around the margins of the @entity3 was predominantly @entity14327 + T cells, which were shown by dual labelling techniques to express mainly GD2 and to a lesser extent GD3. @entity14327 + @entity1573 cells were detected more frequently in cortical regions of the lymph nodes. @entity14328 + T cells were less numerous than @entity14327 + T cells and expressed both GD3 and GD2. Expression of GD2 was also prominent on @entity14327 + T cells, B lymphocytes and @entity1358 in germinal centres, whereas GD3 was mainly expressed on T cells in the margins of the follicles. In contrast to the predominance of @entity14327 + T cells in lymph nodes, @entity14327 + and @entity14328 + T cells were in approximately equal proportions about primary @entity320 and @entity3 in skin. GD2 was largely undetectable on lymphocytes at these sites. In contrast, GD3 was detected on both @entity14328 + and @entity14327 + lymphocytes but not on B lymphocytes. The absence of GD2 on @entity14327 + T cells in skin suggested the latter were a different subpopulation to those in lymph nodes. There appeared to be no clear correlation, however, with subsets of @entity14327 T cells defined by the 2H4 and Leu 8 MAbs.(ABSTRACT TRUNCATED AT 250 WORDS)
