Decrease in fibrin content of venous thrombi in selectin-deficient XXXX .

@entity24780 @entity19 @entity49 @entity1618 @entity715
The purpose of this study was to quantify the fibrin content of thrombi produced in a @entity19 model of @entity49 and correlate this to @entity715 mass. The role of P-selectin, @entity24780 , and @entity1618 on @entity715 fibrin content was analyzed using knockout (KO) @entity19 . Five groups of @entity19 were evaluated: control (N = 10), P-selectin KO (N = 7), @entity24780 KO (N = 5), combined E-/P-selectin KO (N = 12), and @entity1618 KO (N = 10). @entity49 was induced by ligation of the infrarenal IVC. @entity19 were sacrificed on postoperative days (POD) 2 and 6. @entity715 mass was calculated. Sections of IVC were stained with an antibody that cross reacts with @entity19 fibrin. The distribution of RGB color pixels was generated from digitized micrographs of the @entity715 of each animal. The mean pixel value for each group was compiled and analyzed using 2-way ANOVA. Mean pixel value per group was correlated with the mean @entity715 mass per group. Color analysis demonstrated significant decreases in the analyzed fibrin content on POD-2 between the control vs E-/P-selectin KO (P < 0.05) and control vs @entity1618 KO (P < 0.05) groups. In addition, significantly less fibrin staining was noted on POD-6 between the control vs @entity24780 KO (P = 0.03), control vs P-selectin KO (P = 0.01), and control vs E-/P-selectin KO (P < 0.01). There was a strong overall correlation between the mean pixel value for each group and the @entity715 mass (R = 0.964; P < 0.01). This study demonstrates a difference in fibrin content of thrombi produced in animals deficient in @entity24780 , P-selectin, and @entity1618 , supporting their importance in @entity715 , fibrin formation, and the mass of @entity715 formed.
