Secretory phospholipases A2 activate selective functions in XXXX eosinophils.

@entity1 @entity32 @entity1712 @entity650 @entity225 @entity6579 @entity7986 @entity4990 @entity565 @entity3349 @entity1426 @entity1759 @entity3962 @entity223 @entity18471 @entity687 @entity224 @entity7098
Secretory phospholipases A(2) ( @entity18471 ) are released in large amounts in the blood of @entity1 with systemic inflammatory diseases and accumulate at sites of chronic @entity32 , such as the airways of @entity1 with @entity565 . Blood eosinophils or eosinophils recruited in inflammatory areas therefore can be exposed in vivo to high concentrations of @entity7098 . We have examined the effects of two structurally different @entity18471 (group IA and group IIA) on several functions of eosinophils isolated from normal donors and @entity1 with @entity1712 . Both group IA and IIA @entity7098 ) induced a concentration-dependent release of beta-glucuronidase, @entity650 , and @entity687 . Release of the two cytokines was associated with the accumulation of their specific mRNA. In addition, @entity18471 induced the surface expression of @entity1759 and @entity7986 , two major activation markers of eosinophils. In contrast, none of the @entity18471 examined induced the production of @entity225 , the de novo synthesis of @entity223 and platelet-activating factor, or the generation of @entity224 anion from @entity1 eosinophils. Incubation of eosinophils with the major enzymatic products of the @entity18471 ( @entity1426 , @entity6579 , or @entity4990 ) did not reproduce any of the enzymes' effects. In addition, inactivation of @entity7098 ) enzymatic activity by @entity3349 did not influence the release of beta-glucuronidase or of cytokines. Stimulation of eosinophils by @entity18471 was associated with activation of @entity3962 . These results indicate that @entity18471 selectively activate certain proinflammatory and immunoregulatory functions of @entity1 eosinophils through mechanism(s) independent from enzymatic activity and from the generation of @entity1426 .
