In vitro growth rate of fibroblast-like synovial cells is reduced by XXXX treatment.

@entity1 @entity571 @entity625 @entity66 @entity1500 @entity5145 @entity1499 @entity309
BACKGROUND: Fibroblast-like synovial cells (FLS) can be cultured and expanded in vitro in monolayer. Little is known about the growth characteristics of FLS derived from different @entity1 . OBJECTIVE: To study FLS cultures, with particular attention to differences in growth rate of FLS from @entity1 with @entity309 ( @entity309 ) and from other arthritic @entity1 . Additionally, to analyse the influence of @entity1499 ( @entity1500 ) treatment, @entity1 age, and disease duration on FLS growth characteristics. MATERIALS AND METHODS: FLS were isolated from needle arthroscopy biopsy specimens. Twenty four @entity1 (11 @entity309 , 8 @entity5145 , 1 @entity571 , and 4 undifferentiated @entity625 ) were studied. FLS population doubling time was determined between passage 2 and passage 5. Differences in population doubling time between @entity309 and non- @entity309 FLS and between FLS from @entity1 receiving @entity1500 and those not receiving this drug were analysed. In addition, possible correlations between FLS population doubling time and @entity1 age or disease duration were examined. RESULTS: In vitro monolayer FLS cultures from needle arthroscopy biopsy specimens showed linear growth characteristics. Cell growth rate was not correlated with @entity66 . Cells from @entity1 undergoing treatment with @entity1500 showed a longer population doubling time than FLS from @entity1 not receiving this drug (Mann-Whitney test, p<0.05). No correlation was found with @entity1 age or disease duration. CONCLUSION: The results suggest that FLS growth in monolayer is not dependent on the disease affecting the joint. @entity1500 treatment, however, was more relevant in determining FLS growth rate.
