Circulating malignant cells in XXXX : correlation with binding by peanut agglutinin.

@entity1 @entity706 @entity8624 @entity1892 @entity1893
A significant number of @entity1 with @entity1893 have peripheral blood involvement during the course of their disease. Because the expression of receptor for the lectin peanut agglutinin PNA by normal lymphocytes is associated with noncirculating (stationary phase) cells, we studied the relationship between PNA binding by @entity706 cells and the presence of clonal B cells in the blood of 38 @entity1 with @entity1892 . The binding of PNA by cells in tissues was determined by the immunoperoxidase method and by two-color flow cytometry. @entity706 cells (clonal B cells) were identified by a sensitive flow-cytometric technique (kappa-lambda analysis) and were also studied for PNA binding in some cases. In all, 16 of 38 (42%) of @entity706 were PNA+, including a spectrum of histologic types. @entity706 cells were demonstrated in 17 of 22 @entity706 , whereas only 3 of 16 of PNA+ @entity706 had such circulating cells. Thus, there is a significant association between PNA binding and peripheral blood involvement by @entity706 (P less than .005 by chi-square analysis). In 12 cases, the circulating and tissue @entity706 cells had similar expression of PNA receptor (2 PNA+ and 10 PNA- cases), indicating that modulation of the PNA binding sites did not occur. In three @entity1 who presented with @entity8624 , the circulating malignant cells were PNA-. These findings suggest that for both normal and malignant lymphocytes the absence of binding sites for PNA is associated with the capacity of these cells to circulate freely.
