XXXX lung telocytes could promote the proliferation and angiogenesis of @entity1 pulmonary microvascular endothelial cells in vitro.

@entity1 @entity7 @entity2277 @entity2467 @entity2280
BACKGROUND: In the previous studies, telocytes were found near the capillaries in many tissues, especially on the extracellular matrix of blood vessels and positive to @entity2280 and @entity2467 . Therefore, the present study aimed to explore if telocytes could produce angiogenesis associated cytokines, promote the proliferation and the angiogenesis of vascular endothelial cells in vitro. METHODS: @entity1 lung telocytes were isolated and cultured, and were identified by immunofluorescence cytochemistry with @entity2280 , @entity2467 and @entity2277 . Telocytes conditional media (TCM) was prepared, and the expressions of angiogenesis associated cytokines in TCM were detected by ELISA. @entity1 pulmonary microvascular endothelial cells (HPMECs) were cultured with DMEM media or TCM for 72  hours. The proliferation of HPMECs was continuously detected with CCK-8 kit at an interval of 12  hours. HPMECs were also injured by lipopolysaccharide, and cultured with TCM and DMEM respectively, and the tube formation capacity was detected. RESULTS: Telocytes were positive for @entity2280 , @entity2467 and @entity2277 . The expressions of @entity7 and EGF in TCM were significantly higher, the proliferation of HPMECs cultured with TCM significantly increased, and the tube formation of HPMECs injured by endotoxin was improved with the culture of TCM, as compared with the culture of DMEM. CONCLUSION: The present study provides the evidence that @entity1 lung telocytes could produce the growth factors, such as @entity7 and EGF. Telocytes conditional media induced the proliferation of pulmonary endothelial cells and prevented from endotoxin-induced compromise of pulmonary endothelial angiogenesis.
