Bone morphogenetic proteins are mediators of luteolysis in the XXXX corpus luteum.

@entity1 @entity3823 @entity1841 @entity624 @entity13062 @entity1465 @entity4819 @entity23385 @entity35859 @entity11673 @entity1842 @entity30713 @entity10548
Bone morphogenetic proteins (BMPs), members of the transforming growth factor b (TGFb) superfamily, play important roles in folliculogenesis in various species; however, little is known about their role in luteal function. In this study, we investigated the expression, regulation, and effects of @entity1841 , @entity13062 , and @entity11673 in carefully dated @entity1 corpora lutea and cultured @entity1 luteinized granulosa cells. The mRNA abundance of BMPs was increased in the regressing corpus luteum in vivo (P < .01-.001). @entity1 @entity1465 down-regulated @entity1841 , @entity13062 , and @entity11673 transcripts both in vivo (P = .05-.001) and in vitro (P < .001), and decreased the mRNA abundance of BMP receptors ( @entity1842 , @entity30713 , @entity3823 ; P < .05-.01) in vitro. Three BMPs were regulated by differential signaling pathways. H89, a protein kinase A inhibitor, increased the expression of both @entity1841 (P < .05) and @entity13062 (P < .05) while decreasing @entity11673 (P < .01). PMA, a protein kinase C activator, decreased both @entity13062 and @entity11673 expression (P < .0001) while enhancing the mRNA abundance of @entity1841 (P < .01). BMPs significantly down-regulated transcripts for @entity10548 ( @entity10548 ; P < .001) and @entity624 ( @entity624 ; P < .001), whereas up-regulating those of @entity23385 ( @entity23385 ; P < .01) and aromatase ( @entity4819 ; P < .05-.01) in vitro, possessing an effect opposite to hCG but similar to Activin A. Like Activin A, @entity13062 and @entity11673 stimulated the expression of Inhibin/Activin subunits with a marked effect on @entity35859 expression (P < .05-.01). These data confirm that BMPs are increased during luteal regression and negatively regulated by hCG via differential mechanisms, suggesting that BMPs are one of the mediators of luteolysis in @entity1 .
