Increased ratio of dietary XXXX to protein shifts the focus of metabolic signaling from skeletal muscle to adipose.

@entity2308 @entity3363 @entity2508 @entity1735 @entity35 @entity14446 @entity435 @entity413
BACKGROUND: The Dietary Reference Intakes (DRI) established acceptable macronutrient distribution ranges (AMDR) for @entity3363 and protein, however little is known about differences in glycemic regulations and metabolic signaling across this range. This study examined metabolic outcomes associated with intake of two diets differing in @entity1735 :protein ratios representing the upper and lower ends of the AMDR. METHODS: Adult, male @entity35 were fed either a high @entity1735 ( @entity435 ) diet (60% of energy from @entity3363 , 12% protein, 28% fat; n = 30) or a high protein (PRO) diet (35% @entity1735 , 35% protein, 30% fat; n = 30). @entity35 were meal-fed 3x/d the respective diets for 10 d and then terminated after overnight food deprivation or 30, 60, 90, 120 min post-prandial (PP). Plasma was collected at each of these points to provide a time course for @entity413 , insulin and C-peptide. Skeletal muscle and adipose tissues were collected at 0, 30 and 90 min for measurements of basal, early and delayed activation of @entity2308 , @entity14446 and @entity2508 . Data were analyzed by two-way ANOVA. RESULTS: The @entity435 group produced a consistently elevated response in plasma @entity413 , insulin and C-peptide following the meal through the 120 min time course. In addition, @entity2308 and @entity2508 activation in adipose was much higher than in skeletal muscle. Conversely, the PRO group PP @entity413 response was minimal and insulin maintained a response similar to a biphasic pattern. Tissue responses for the PRO group were greater for @entity2308 and @entity14446 signaling in skeletal muscle compared with adipose. CONCLUSION: Taken together these data suggest that altering @entity435 :PRO ratios within the AMDR produce different glycemic response patterns accompanied by differential metabolic signaling in skeletal muscle and adipose.
