Differences in long-term effects of XXXX and @entity413 on insulin release from @entity35 pancreatic islets.

@entity1160 @entity368 @entity9640 @entity35 @entity413
We have compared the effects of long-term exposure to @entity9640 or @entity413 on nutrient-induced insulin release from pancreatic islets of the @entity35 . After 3 days of culture islets were finally tested in 1 h incubations for insulin responses to 16.7 mM of @entity413 , @entity1160 , @entity368 or a combination of @entity368 and @entity1160 . After culture at 11 mM @entity413 + 2 mM @entity1160 (A), @entity413 , @entity368 and @entity1160 stimulated release to a similar extent from islets. After culture at 1.7 mM @entity413 + 10 mM @entity1160 (B), only @entity368 stimulated insulin release. After culture at 11 mM @entity413 + 10 mM @entity1160 (C), both @entity368 and @entity1160 increased the insulin response. After culture at 1.7 mM @entity413 and 2 mM @entity1160 (D), only @entity1160 slightly stimulated release. After culture in high @entity1160 (B or C), a combination of @entity368 and @entity1160 significantly inhibited release as compared to @entity368 alone. A switch in culture media from B to A for 1 h prior to final incubations revived insulin release in response to @entity413 but not to @entity1160 . The reverse switch (A to B) abolished both subsequent @entity413 -and @entity1160 -induced insulin release. A switch from D to B revived an insulin response to @entity368 . Exposure of B-cells to 11 mM @entity413 during 30 min in another experimental system (perfused pancreas) induced a significant insulin response to subsequent stimulation with @entity1160 ; this response was, however, only 17% of that to @entity413 per se observed in the same experiments.(ABSTRACT TRUNCATED AT 250 WORDS)
