DNA aberrations in the epithelial cell component of XXXX .

@entity1 @entity7197 @entity242 @entity5 @entity52362 @entity876 @entity74 @entity9051
@entity52362 is a rare @entity5 composed of cells with epithelial characteristics in various differentiation patterns surrounded by fibrous cells. Evidence as to whether this @entity5 should be designated as an epithelial bone @entity5 or a @entity242 with both epithelial and mesenchymal features is lacking. In this study the nature of the mesenchymal and epithelial components of @entity7197 was investigated by DNA flow cytometry, DNA image cytometry, @entity876 immunohistochemistry, and polymerase chain reaction-based loss of heterozygosity detection at the @entity876 locus. Specimens from 6 of 15 @entity1 (40%) analyzed by flow cytometry had an aneuploid DNA index. Image cytometry analysis of Feulgen-stained paraffin sections of 6 aneuploid and 2 diploid @entity5 revealed that aneuploid nuclei were detected in cells with an epithelial phenotype only, whereas all fibrous cells were diploid. Immunohistochemistry for @entity876 on specimens from 25 @entity1 revealed moderate or strong immunoreactivity in 12 @entity5 (48%) restricted to the epithelial cells. Loss of heterozygosity at the @entity876 locus could be confirmed in the epithelial component of an immunohistochemically @entity876 -positive @entity5 . Additionally, sections of 7 lung metastases were studied histologically. Only keratin-positive epithelial cells, predominantly in the spindle cell pattern, were present in these metastases, whereas the osteofibrous tissue present in the @entity5 was not detected. These results suggest that either @entity7197 consists of a malignant epithelial part with a reactive @entity9051 or that the malignant epithelial cells develop next to a proliferating benign fibrous component. Additional analysis of common @entity74 in the fibrous and epithelial cells of @entity7197 is therefore indicated.
