Monoclonal antibodies reactive with XXXX peritoneal macrophages: identification of macrophage heterogeneity.

@entity742 @entity1669 @entity1735 @entity1573 @entity465
Mouse @entity465 ( @entity465 ) were prepared against @entity1573 inflammatory macrophages for the purpose of analyzing macrophage heterogeneity. Macrophage-rich adherent peritoneal exudate cells harvested from Cornell K-strain @entity1573 42 hr after stimulation with Sephadex were used as immunogens in the production of the @entity465 . Eight hybridoma clones producing antibodies reactive with @entity1573 peritoneal macrophages were subjected to characterization. While six of the @entity465 cross-reacted with various hematopoietic cell types, two @entity465 (CMTD-1 and -2) were restricted in reactivity to macrophages. CMTD-1 was found to react with activated peritoneal macrophages generated by several irritants. In contrast, CMTD-2 identified a subpopulation of macrophages elicited by specific @entity1735 -based stimulants. This presumably was based on the cross-reaction of this MCA with specific @entity1735 linkages. Analysis using flow cytometry revealed the time-dependent appearance of CMTD-2-positive peritoneal macrophages between 24 and 52 hr after ip Sephadex injection. This subpopulation of peritoneal macrophages was found to be heterogeneous for the ability to undergo in vitro phagocytosis of @entity742 erythrocytes. CMTD-2-positive cells were also detected in the thyroids of 2-week-old Obese strain @entity1573 with spontaneous @entity1669 and at a low incidence in the spleens of normal @entity1573 .
