Progressive genetic alterations of XXXX with high-grade transformation.

@entity5 @entity2347 @entity517 @entity406 @entity876 @entity3 @entity1722
CONTEXT: Although genome-wide imbalances have been characterized in conventional @entity2347 , other than @entity876 mutational status, the molecular profile of @entity2347 with high-grade transformation has not been explored. OBJECTIVE: To evaluate progressive genetic alterations in @entity2347 with high-grade transformation using array comparative genomic hybridization. DESIGN: Five @entity2347 with high-grade transformation (4 primary @entity5 and 1 paired @entity3 ) were selected and characterized at the DNA level by array comparative genomic hybridization on @entity406 -fixed paraffin-embedded tissue. Select alterations were validated by fluorescence in situ hybridization. RESULTS: Chromosomal gains were mostly confined to the areas of high-grade transformation while losses were seen only in the conventional areas. Chromosomal regions with significant gains included 8q24, 17q11.2-q12, 17q23, and 15q11-13. Regions that showed the significant losses included 9q34, 4p16, 1p36.1, and 11q22. Fluorescence in situ hybridization analysis demonstrated increases in @entity517 (8q24.12-q24.13) and a low level increases in @entity1722 ( formerly @entity1722 ) (17q11.2-q12) in cases showing gains by array comparative genomic hybridization in these regions. However, no @entity5 showed @entity1722 / @entity1722 immunopositivity. CONCLUSIONS: High-grade transformation in @entity2347 is a complex process that is reflected by several chromosomal alterations. Our findings implicate @entity517 amplification in this progression, although the role of @entity1722 /neu is still unclear. Other candidate oncogenes, particularly on chromosome 17q23, warrant investigation in this rare @entity5 .
