Inhibitory effects of Schefflera leucantha extract on production of XXXX mediators by Langerhans cells and mast cells.

@entity483 @entity19 @entity565 @entity794 @entity638 @entity56 @entity15064 @entity112 @entity1068 @entity970
BACKGROUND: Schefflera leucantha Viguier is used as a traditional medicine in Thailand and China to relieve chronic cough and @entity565 . However, little is known about its anti- @entity56 effects. OBJECTIVE: This study was designed to investigate the effects of S leucantha @entity1068 extract (SLEE) on chemokine production by epidermal Langerhans cells (LCs) stimulated with peptidoglycan ( @entity970 ) from @entity794 and @entity112 release from mast cells. METHODS: LCs were purified from @entity19 epidermal cells using the panning method with anti-IA(d) monoclonal antibody. Chemokine production by LCs was investigated by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA). Mast cells for @entity112 release assay were induced by long-term culture of @entity19 spleen cells. @entity112 release from these mast cells was measured by a competitive ELISA. RESULTS: Production of the eosinophil chemoattractant @entity638 and the type 2 T helper (TH2)-associated chemokine @entity15064 from @entity970 -stimulated LCs was significantly inhibited by SLEE. Furthermore, SLEE significantly decreased the release of @entity112 from mast cells by IgE-mediated degranulation. CONCLUSION: These results suggest that S leucantha may offer a new therapeutic approach for the control of @entity483 associated with @entity794 colonization through inhibition of the production of @entity56 mediators.
