High-throughput pyrosequencing of the chloroplast genome of a highly neutral-lipid-producing marine pennate diatom, XXXX .

@entity73341 @entity73340 @entity34466 @entity563 @entity73342
The chloroplast genome of the highly neutral-lipid-producing marine pennate diatom @entity73340 was fully sequenced using high-throughput pyrosequencing. The general features and gene content were compared with three other complete diatom chloroplast genomes. The chloroplast genome is 134,918 bp with an inverted repeat of 13,330 bp and is slightly larger than the other diatom chloroplast genomes due to several low gene-density regions lacking similarity to the other diatom chloroplast genomes. Protein-coding genes were nearly identical to those from @entity34466 . On the other hand, we found unique sequence variations in genes of photosystem II which differ from the consensus in other diatom chloroplasts. Furthermore, five functional unknown ORFs and a putative @entity563 recombinase gene, @entity73341 , are located in the low gene-density regions. @entity73341 was also identified in the plasmids of another pennate diatom, Cylindrotheca fusiformis, and in the plastid genome of the diatom endosymbiont of @entity73342 . Exogenous plasmids might have been incorporated into the chloroplast genome of @entity73340 sp. by lateral gene transfer. Chloroplast genome sequencing analysis of this novel diatom provides many important insights into diatom evolution.
