Patterns of DNA amplification at band q13 of chromosome 11 in human XXXX .

@entity0 @entity5 @entity4191 @entity10008 @entity10006
In an attempt to verify the nature of amplification events at band q13 on chromosome 11 we surveyed the amplification status of ten molecular markers specific for this region ( @entity4191 , SEA, D11S97, D11S146, BCLI, PRADI/CCNDI, @entity10008 / @entity10008 , @entity10006 / @entity10006 , EMSI, and DIIS833E) in a panel of 389 primary @entity0 DNA samples. Eighty-eight @entity5 (23%) showed at least one of these markers amplified, but in a majority of the cases amplification encompassed more than one of the tested loci. Our data confirm that amplicons at 11q13 can cover large portions of DNA and are consistent with the existence of several cores of amplification. One important core seems to be, as previously described, centered around PRADI/CCNDI; 57 @entity5 (14.7%) showed amplification at PRADI/CCNDI either alone (one @entity5 ) or along with amplification of BCLI or @entity10006 / @entity10006 . The level of amplification of PRADI/CCNDI sometimes exceeded that of surrounding markers. Three additional amplification events occurring independently of amplification of PRADI/CCNDI were also detected. Centromeric to BCLI, probes to DIIS97, and DIIS146 detected amplification in 60 @entity5 (15.4%) and were often the only amplified markers. Telomeric to @entity10006 / @entity10006 , DIIS833E was found amplified alone in ten @entity5 , and it was the most amplified marker in another six cases. At a shorter distance of @entity10006 / @entity10006 , EMSI was the only amplified marker in two @entity5 , with a level of amplification that could exceed that of PRADI/CCNDI and DIIS833E. Our data thus suggest the existence of four independent amplified regions within band 11q13 in @entity0 .
