Effect of @entity842 gas on the generation of XXXX by isolated blood vessels: implications for inhalation therapy.

@entity1 @entity141 @entity649 @entity2647 @entity842 @entity35 @entity3623
1. We have investigated, using @entity35 aortic rings, whether exogenous @entity842 (NO) gas affects the activity or expression of the inducible, @entity141 -independent NO synthase. 2. Incubation of rings with lipopolysaccharide (LPS, S. typhosa) for 6 h resulted in a gradual loss of tissue tone, a time-dependent reduction in constrictor response to @entity2647 and significant expression and activity of @entity141 -independent NO synthase. 3. Following incubation of LPS-treated rings with NO gas, the expression of @entity3623 mRNA was still observed, although the enzyme activity was significantly reduced and there was no reduction in the response to @entity2647 . 4. Therefore, NO gas can inhibit the action but not the induction of an NO synthase likely to play a role in inflammatory states such as @entity649 ( @entity649 ). 5. These observations may explain the rebound phenomenon observed in some @entity649 @entity1 following inhalation therapy with NO gas.
