Activation of serotonergic neurons during XXXX -induced @entity392 .

@entity750 @entity392 @entity1639 @entity2697
OBJECTIVES/HYPOTHESIS: In gerbils in which @entity392 was induced by @entity2697 , neurons in the dorsal raphe nucleus were activated. Since about half the neurons in this region are serotonergic, this indicates that @entity750 ( @entity750 ) might play a role in the mechanisms of central @entity392 . The goal of this study was to determine if serotonergic neurons are activated during @entity2697 -induced @entity392 . Further, to determine if the same neurons might modulate the cochlea during @entity392 , neuroanatomical tract-tracing with @entity750 immunohistochemistry was used to determine if serotonergic neurons project to the gerbil cochlea. STUDY DESIGN: Randomized, prospective, gerbils. METHODS: Six gerbils were injected with @entity2697 (saline for controls). Four hours later, the gerbils were euthanized, perfused, and their brains collected for immunohistochemical labeling of @entity750 and @entity1639 . For the tract-tracing, FluoroGold was injected into the cochleae of 3 gerbils. The gerbils were euthanized and perfused 4-11 days later and the brains immunohistochemically processed for @entity750 . RESULTS: More serotonergic neurons expressed @entity1639 in the @entity2697 -injected animals compared to controls. The increase was significant for 3 of the 8 major serotonergic cell groups including B7, B9, and the caudal linear nucleus. Despite robust labeling of olivocochlear and vestibular efferents with FluoroGold, @entity750 -labeled neurons containing FluoroGold were lacking. CONCLUSIONS: @entity2697 -induced @entity392 activates serotonergic neurons in rostral cell groups. Activation of these neurons is not likely to influence cochlear function directly, but is likely to influence a number of auditory and non-auditory regions known to be involved with @entity392 .
