Cellular localization of tissue inhibitors of metalloproteinases in the XXXX ovary throughout pseudopregnancy.

@entity6930 @entity42 @entity11018 @entity35 @entity11019 @entity8665
The present study determined the ovarian cellular localization of the mRNA for the tissue inhibitors of metalloproteinases (TIMPs) during pseudopregnancy in the @entity35 . Pseudopregnancy was induced by eCG/hCG stimulation. At Day 1 of pseudopregnancy, intense reaction product for @entity6930 mRNA was observed surrounding the developing @entity42 ( @entity42 ), with less intense expression present in granulosa-lutein cells. With continued luteal development, the @entity6930 mRNA encircling the @entity42 was lost, although low levels of expression were found within the @entity42 . For @entity11018 mRNA, intense reaction product was observed surrounding the developing @entity42 but, unlike @entity6930 , was present in granulosa- @entity8665 cells, with high levels near the center of the @entity42 . The localization pattern of @entity11018 mRNA was unchanged through the latter stages of pseudopregnancy. @entity11019 mRNA expression was strikingly different from the other TIMPs. At Day 1 of pseudopregnancy, intense reaction product for @entity11019 mRNA was observed in granulosa-lutein cells of certain developing @entity42 , whereas adjacent follicles did not express @entity11019 mRNA. With continued luteal development, there was a homogenous, intense localization of @entity11019 mRNA throughout the @entity42 , which was unchanged during pseudopregnancy. To understand the induction of @entity11019 mRNA in the developing @entity42 , a series of experiments was performed to compare markers of follicular maturity with the presence of @entity11019 mRNA. @entity11019 mRNA appears to be switched on in granulosa cells of follicles destined to ovulate. The distinct pattern of expression of the three TIMPs suggests that each inhibitor may regulate either the site and extent of proteolytic action or specific matrix metalloproteinases at different periods of the luteal life span.
