[Cellular culture of osteoblasts and fibroblasts on porous XXXX - @entity1391 bone substitutes].

@entity9039 @entity78 @entity692 @entity79 @entity98 @entity1391 @entity460
PURPOSE OF THE STUDY: @entity78 ceramics are synthetic bone substitutes able to fill in @entity79 as a support of the bone growth. This work consisted in an in vitro assessment of osteoblasts and fibroblasts cultures on macroporous @entity460 - @entity1391 bone substitutes to analyze the interaction between cells and bone substitute. MATERIALS AND METHODS: The macroporous ceramic was composed of 70% @entity692 and 30% @entity9039 with known mechanical and physico-chemical properties. Three compounds were processed with different size of macropore and with or without microporosity on their surface. Cells were seeded on discs measuring 10 mm in diameter and 2 mm in thickness. Cellular viability was evaluated by the @entity98 test for every stage of observation. An histological study to observe the invasion in the depth of discs was performed. Scanning electron microscopy was used to analyze the cellular comportment in contact with the surface of substitutes. RESULTS: An exponential cellular growth was effective on each substitute with the two cellular types. Cells spread on the surface of the compounds covering macropores and colonized the depth of the discs. A size of macropore of 300 microm or more seemed to support this invasion. 15 microm sized interconnections appeared to be effective to allow cell migration between macropores. The cell proliferation was similar on substitutes with or without microporosity. CONCLUSION: Biomaterials currently used as bone substitute are more or less osteoconductive but they have no osteoinductive property. A hybrid association of @entity78 ceramic with osteogenic cells should promote the development of a @entity78 compound with osteoinductive capacity.
