Immunological study of the heat-labile enterotoxins of XXXX and Vibrio cholerae.

@entity1 @entity1329 @entity53539 @entity2624 @entity526
Immunodiffusion experiments were conducted to associate a precipitin line with @entity526 heat-labile enterotoxin (LT). Wild strains of porcine and of @entity1 enteropathogenic @entity526 as well as laboratory-derived enterotoxigenic variants of @entity2624 were used for LT antigen preparations. These were produced mainly by ultrafiltration and @entity1329 precipitation of broth culture supernatants. When antisera with anti-LT activity were reacted with antigen preparations from Ent(+) and Ent(-) variants of @entity2624 , a line "a" was given by Ent(+) but not by Ent(-) preparations. Line "a" was removed by absorption of anti-LT serum with antigen preparation from an Ent(+) @entity2624 , but was unaffected when the antigen preparation used to absorb the serum was from an Ent(-) @entity2624 . A line identical to "a" was given by antigen preparations from wild strains of porcine enteropathogenic @entity526 reacted with homologous or heterologous anti-LT sera. One @entity1 of enteropathogenic @entity526 was shown to possess an antigen identical to that which gave rise to line "a." To demonstrate this line it was necessary to use high concentrations of gammaglobulin and high concentrations of the crude antigen preparations. LT preparations reacted with anticholera toxin to give a line "c," which showed a reaction of partial identity with line "b" produced by reaction of pure @entity53539 and anticholera toxin. Lines "a" and "c" gave reactions of identity.
