Diffusion-weighted imaging and dynamic contrast-enhanced MRI of experimental XXXX - A correlation study with histology.

@entity5 @entity3 @entity0 @entity7241
PURPOSE: To validate imaging parameters from diffusion-weighted imaging and dynamic contrast-enhanced MRI with immunohistology and to non-invasively assess microstructure of experimental @entity0 . MATERIALS AND METHODS: Animals bearing @entity0 were imaged in a clinical 1.5T MRI scanner. HASTE sequences were performed to calculate apparent diffusion coefficients. Saturation recovery turbo FLASH sequences were conducted while infusing 0.1mmol/l @entity7241 for dynamic contrast-enhanced MRI to quantify parameters amplitude A and exchange rate constant kep. After imaging, bone @entity3 were analyzed immunohistologically. RESULTS: We found correlations of the apparent diffusion coefficients from diffusion-weighted imaging with @entity5 cellularity as assessed with cell nuclei staining. Histological vessel maturity was correlated negatively with parameters A and kep from dynamic contrast-enhanced MRI. @entity5 size correlated inversely with cell density and vessel permeability as well as positively with mean vessel calibers. Parameters from the rim of bone @entity3 differed significantly from values of the center. CONCLUSION: In vivo diffusion-weighted imaging and dynamic contrast-enhanced MRI in experimental bone @entity3 provide information about @entity5 cellularity and vascularity and correlate well with immunohistology.
