Upregulation of microRNA  -138  -5p inhibits XXXX cell migration and increases chemotherapy sensitivity.

@entity1 @entity788 @entity5 @entity189 @entity2277 @entity1487
UNASSIGNED: The present study investigated the role of microRNA (miR)  -138  -5p in regulating @entity189 migration and sensitivity to chemotherapy in @entity788 . Reverse transcription  -quantitative polymerase chain reaction (RT  -qPCR) was used to assess the expression levels of miR  -138  -5p in @entity788 cell lines and @entity189 tissues from @entity1 @entity1 . A lentiviral vector, containing miR  -138  -5p mimics (lv  -miR  -138  -m) or miR  -138  -5p inhibitor (lv  -miR  -138  -i), was used to either upregulate or downregulate the expression levels of miR  -138  -5p in PANC  -1 cells, respectively. The effects of miR  -138  -3p regulation on @entity788 cell migration and sensitivity to chemotherapy were examined. The predicted targeting of miR  -138  -5p on @entity2277 ( @entity2277 ) was assessed by western blotting in PANC  -1 cells. @entity2277 was subsequently downregulated using small interfering (si)RNA to determine its effect on miR  -138  -5p  -modulated @entity788 cell development. The expression levels of miR  -138  -5p were downregulated in @entity788 cell lines and @entity189 tissues. In PANC  -1 cells, lentivirus-mediated upregulation of miR  -138  -5p inhibited @entity5 cell migration and increased cell chemosensitivity to @entity1487 ( @entity1487 ). By contrast, downregulation of miR  -138  -5p promoted @entity5 cell migration and decreased cell chemosensitivity to 5  -FU. A luciferase assay revealed that @entity2277 was a direct target of miR  -138  -5p. Western blotting demonstrated that @entity2277 was downregulated upon the upregulation of miR  -138  -5p in PANC  -1 cells. siRNA  -mediated downregulation of @entity2277 inhibited @entity788 cell migration in the control and miR  -138  -5p downregulated PANC  -1 cells. The present study demonstrated that miR  -138  -5p is important in regulating @entity788 development, possibly through targeting @entity2277 .
