Gene expression profiling of a pressure-tolerant XXXX Scott A ctsR deletion mutant.

@entity5125 @entity101 @entity2048
@entity5125 is a food-borne pathogen of significant threat to public health. @entity101 ( @entity101 ) treatment can be used to control @entity5125 in food. The CtsR (class three stress gene repressor) protein negatively regulates the expression of class III heat shock genes. A spontaneous pressure-tolerant ctsR mutant 2-1 that was able to survive under @entity101 treatment has been identified previously. So far, there is only limited information about the mechanisms of survival and adaptation of this mutant to high pressure. Microarray technology was used to monitor the gene expression profiles of the ctsR mutant 2-1 under @entity101 treatment. Compared to pressure-treated @entity5125 Scott A wild type, 17 genes were up-regulated (>2-fold increase) in the ctsR mutant 2-1, whereas 58 genes were down-regulated (<-2-fold decrease). The entire clpC operon was up-regulated in the ctsR mutant 2-1, indicating that the mutant CtsR protein was not a functional repressor. The increased levels of expression of stress-related genes in ctsR mutant 2-1 may contribute to its survival under high pressure. The reduced expression levels of the genes related to virulence, flagella synthesis, and cell division in the ctsR mutant 2-1 correlate with its characteristics (elongated cells, reduced virulence, and @entity2048 ). The gene expression changes determined by microarray assays were confirmed by real-time reverse transcriptase PCR analyses. This study enhances our understanding of how @entity5125 survives under @entity101 and may contribute to the design of effective and economically feasible @entity101 treatment in food processing.
