Differential cardioprotective effects of @entity73010 and XXXX on acute @entity583 are mediated by unique signaling pathways.

@entity172 @entity583 @entity2697 @entity63 @entity16217 @entity1188 @entity460 @entity73010 @entity35 @entity27376 @entity65
ETHNOPHARMACOLOGICAL RELEVANCE: @entity73010 ( @entity2697 ) and @entity16217 ( @entity27376 ) are major hydrophilic and lipophilic compounds, respectively, from one herbal medicine, Danshen, which has been widely and successfully used for treating @entity65 in Asian countries. Because few studies have reported different molecular mechanisms between the different compounds in same herb, we investigate if separate molecular pathways are involved in cardioprotective effect by different active components of Danshen. MATERIALS AND METHODS: We used an acute @entity583 ( @entity583 ) model to compare the cardioprotective effects of @entity2697 and @entity27376 in @entity35 . Both @entity1188 size and echocardiographic response were evaluated at 3, 7, 14 and 28 days after surgery. Genes involved in @entity63 and in responses to @entity2697 or @entity27376 treatment in @entity63 hearts were identified by microarray analysis and verified by quantitative real-time RT-PCR. RESULTS: Results showed that both @entity2697 and @entity27376 delay the development of @entity63 by decreasing @entity1188 size and improving systolic function post @entity583 . Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated different kinetics and gene expression profiles by @entity2697 and @entity27376 . @entity2697 acts in a later period after @entity63 , and its effect is probably mediated by downregulation of genes involved in oxidative stress, certain G-protein coupled receptor activities and apoptosis. On the other hand, @entity27376 acts relatively early after @entity63 and its effect is at least in part mediated by inhibition of intracellular @entity460 , cell adhesion and alternative complement pathway. Strikingly, we found that @entity27376 , a recently identified member of selective @entity172 receptor modifier (SERM), indeed regulates genes known to be involved in @entity172 metabolism post @entity583 . CONCLUSIONS: Although both @entity2697 and @entity27376 contribute to the cardioprotective effect of Danshen, there are significant mechanistic and temporal differences between the two: @entity27376 acts at an early stage after @entity63 mainly by inhibition of intracellular @entity460 and cell adhesion pathways whereas @entity2697 acts mainly by down-regulating apoptosis.
