XXXX has biochemical, physiological, and structural effects on the green microalga Ankistrodesmus falcatus: An integrative study.

@entity3406 @entity137 @entity2041 @entity3411 @entity224 @entity3363
UNASSIGNED: In recent years, the release of chemical pollutants to water bodies has increased due to anthropogenic activities. @entity3411 is an essential metal that causes damage to aquatic biota at high concentrations. Phytoplankton are photosynthesizing microscopic organisms that constitute a fundamental community in aquatic environments because they are primary producers that sustain the aquatic food web. @entity3406 @entity137 has not been characterized in all of the affected levels of biological organization. For this reason, the present study evaluated the toxic effects of @entity3406 on the growth of a primary producer, the green microalga Ankistrodesmus falcatus, and on its biochemical, enzymatic, and structural levels. The IC50 (96h) was determined for @entity3411 . Based on this result, five concentrations were determined for additional tests, in which cell density was evaluated daily. At the end of the assay, pigments and six biomarkers, including antioxidant enzymes (catalase [CAT], @entity2041 peroxidase [GPx], @entity224 dismutase [SOD]), and macromolecules (proteins, @entity3363 and lipids), were quantified; the integrated biomarker response (IBR) was determined also. The microalgae were observed by SEM and TEM. Population growth was affected starting at 7.5 gL(-1) (0.028 M), and at 120 gL(-1) (0.450 M), growth was inhibited completely; the determined IC50 was 17 gL(-1). Exposure to @entity3406 reduced the concentration of pigments, decreased the content of all of the macromolecules, inhibited of SOD activity, and increased CAT and GPx activities. The IBR revealed that @entity3411 increased the antioxidant response and diminished the macromolecules concentration. A. falcatus was affected by @entity3406 at very low concentrations; negative effects were observed at the macromolecular, enzymatic, cytoplasmic, and morphological levels, as well as in population growth. @entity3411 @entity137 could result in environmental impacts with consequences on the entire aquatic community. Current regulations should be revised to protect primary producers.
