Targeting the delivery of systemically administered haematopoietic stem/progenitor cells to the inflamed colon using XXXX and platelet microparticle pre-treatment strategies.

@entity5568 @entity71194 @entity19 @entity130 @entity29 @entity273 @entity1960 @entity8787 @entity1049
UNASSIGNED: Haematopoietic stem and progenitor cell (HSC) therapy may be promising for the treatment of @entity29 ( @entity29 ). However, clinical success remains poor, partly explained by limited HSC recruitment following systemic delivery. The mechanisms governing HSC adhesion within inflamed colon, and whether this event can be enhanced, are not known. An immortalised HSC-like line ( @entity71194 ) was pre-treated with @entity273 ( @entity5568 ), activated platelet releasate enriched supernatant (PES) or platelet microparticles (PMPs). Subsequent adhesion was monitored using adhesion assays or in vivo ischaemia-reperfusion (IR) and @entity1960 injured @entity19 colon intravitally. Integrin clustering was determined confocally and cell morphology using scanning electron microscopy. Both injuries resulted in increased @entity71194 within @entity1049 . @entity5568 and PES significantly enhanced adhesion in vitro and in the @entity1960 , but not IR injured, colon. PMPs had no effect on adhesion. PES and PMPs induced clustering of integrins on the @entity71194 surface, but did not alter their expression. Adhesion to the colon is modulated by injury but only in @entity130 can this recruitment be enhanced. The enhanced adhesion induced by PES is likely through integrin distribution changes on the @entity71194 surface. Improving local HSC presence in injured colon may result in better therapeutic efficacy for treatment of @entity8787 .
