Attenuation of enoyl coenzyme  A hydratase  1 expression in XXXX cells using small interfering  RNA inhibits cell proliferation and migration.

@entity1 @entity844 @entity0 @entity69356 @entity5 @entity584 @entity14 @entity9459
UNASSIGNED: @entity14 is one of the most commonly diagnosed types of @entity5 and is a leading cause of @entity5  -associated mortality worldwide. Short chain enoyl coenzyme  A hydratase  1  ( @entity69356 ) is an important gene involved in the mitochondrial @entity844 b  -oxidation pathway. In addition, @entity69356 has been implicated in a variety of @entity5 , including @entity0 . The aim of the present study was to examine the expression of @entity69356 in the @entity1 HCT  -8 @entity14 cell line. The results showed that @entity69356 expression was significantly increased in poorly  -differentiated cells compared with that in well  -differentiated cells. In order to further investigate the functions of @entity69356 in @entity14 cells, a stably transfected HCT  -8 cell line expressing small interfering  (si)RNA targeting the @entity69356 gene was established. The expression of the @entity69356 siRNA was found to reduce @entity69356 protein levels in @entity69356  -silenced cells by >40%. Cell proliferation and cell migration of the siECHS1 cells were characterized using Cell Counting Kit  -8 and Transwell assays, respectively, the results of which showed that the constitutive knockdown of the ECSH1 gene in HCT  -8 cells significantly inhibited cell proliferation and migration. Furthermore, decreased levels of @entity584 and glycogen synthase kinase (GSK)3b phosphorylation were observed in @entity69356  -silenced HCT  -8 cells compared with that of parental or pU6 empty vector  -transfected cells. In conclusion, the results of the present study suggested that @entity69356 may have an important role in @entity14 cell proliferation and migration via activation of @entity584  - and @entity9459  -associated signaling pathways.
