Novel sulI binary vectors enable an inexpensive foliar selection method in XXXX .

@entity1501 @entity9795 @entity286 @entity4766 @entity11682 @entity958 @entity415
BACKGROUND: @entity11682 resistance is conferred by the sulI gene found on many Enterobacteriaceae R plasmids and Tn21 type transposons. The sulI gene encodes a @entity11682 insensitive @entity9795 synthase enzyme required for @entity1501 biosynthesis. Transformation of @entity286 , @entity415 or @entity958 using sulI as a selectable marker generates @entity4766 -resistant plants. Typically sulI-based selection of transgenic plants is performed on tissue culture media under sterile conditions. FINDINGS: A set of novel binary vectors containing a sulI selectable marker expression cassette were constructed and used to generate transgenic @entity958 . We demonstrate that the sulI selectable marker can be utilized for direct selection of plants grown in soil with a simple foliar spray application procedure. A highly effective and inexpensive high throughput screening strategy to identify transgenic @entity958 without use of tissue culture was developed. CONCLUSION: Novel sulI-containing Agrobacterium binary vectors designed to over-express a gene of interest or to characterize a test promoter in transgenic plants have been constructed. These new vector tools combined with the various beneficial attributes of @entity11682 selection and the simple foliar screening strategy provide an advantageous alternative for plant biotechnology researchers. The set of binary vectors is freely available upon request.
