@entity136 impacts differentially on XXXX cells: distinct modulations in the expression patterns of apoptosis-related microRNAs and @entity588 family genes.

@entity1 @entity21175 @entity589 @entity588 @entity3373 @entity5 @entity34487 @entity12982 @entity15743 @entity98 @entity150 @entity136 @entity26365 @entity703
UNASSIGNED: Bladder and @entity703 are two representative cases of @entity5 that respond differentially to @entity136 . Previous studies have shown that @entity136 can trigger apoptosis in various @entity5 cells. Herein, we sought to investigate the impact of @entity136 on the expression levels of the @entity588 family members @entity588 , @entity589 , and @entity34487 and the apoptosis-related microRNAs @entity21175 , @entity26365 , @entity3373 , and @entity15743 in the @entity1 @entity150 cell lines @entity1 and @entity1 , respectively. @entity5 cells' viability as well as the IC50 doses of @entity136 were estimated by the @entity98 assay, while the detection of cleaved @entity12982 via Western blotting was used as an indicator of apoptosis. Furthermore, @entity1 and @entity1 cells' ability to recover from treatment was also monitored. Two different highly sensitive quantitative real-time RT-PCR methodologies were developed in order to assess the expression levels of @entity588 family genes and microRNAs. Exposure of @entity5 cells to @entity136 produced the IC50 values of 30 and 3 nM for @entity1 and @entity1 cells, correspondingly, while cleaved @entity12982 was detected only in @entity1 cells. @entity1 cells demonstrated the ability to recover from @entity136 treatment, whereas @entity1 cells' recovery capability was dependent on the initial time of exposure. @entity588 and @entity589 were significantly modulated in treated @entity1 cells. Instead, @entity1 cells exhibited alterations only in the latter, as well as in all studied microRNAs. Therefore, according to our data, bladder and @entity703 cells' response to @entity136 is accompanied by distinct alterations in the expression levels of their apoptosis-related genes and microRNAs.
