The study of influencing factors on the growth characters of XXXX retinal neurons in vitro.

@entity5731 @entity2479 @entity2592 @entity30383 @entity35
PURPOSE: To investigate the influencing factors in culturing Sprague-Dawley (S-D) @entity35 retinal neurons in order to lay foundation for further experimental research. MATERIALS AND METHODS: Retinal cells were plated on plastic plates and coverslips coated with @entity5731 or @entity30383 polymer for primary culture. The cultured cells were divided into following groups: 1. Culture medium changed every 2 to 3 days vs changed only once; 2. @entity2592 ( @entity2592 ) added to the culture medium vs not added. The cells were observed and pictured under inverted phase contrast microscope. The cells were identified through immunocytochemistry. RESULTS: The immunofluorescence showed that most of the cultured cells were neurons, among them were a few @entity2479 cells. In the cultured group of which substrata coated with @entity5731 and culture medium added with @entity2592 , the neurons intended to aggregate into clusters with relatively straight neurites. In the group of which substrata coated with @entity30383 polymer and medium added with @entity2592 , the neurons grew dispersively with bent neurites. Both of them survived for 2 to 3 weeks. The cells which plated in the medium not added with @entity2592 did not aggregate into clusters and survived longer than 4 weeks. In the group of which medium changed several times, the survival time of neurons was shorter than that in the medium changed only once. CONCLUSIONS: The retinal neurons plated on the substrata coated with @entity30383 polymer are easy to observe because of its dispersive growth. It is not favorable for the growth of the neurons by changing culture medium many times. @entity2592 may possibly have side effect on the growth of retinal neurons.
