The uptake of XXXX by RAW 264.7 macrophages.

@entity1 @entity3276 @entity270 @entity5629 @entity1881 @entity4793 @entity2076
BACKGROUND: @entity1881 and @entity2076 are the two major forms of @entity270 in @entity1 plasma and the primary lipid soluble antioxidants. The dietary intake of @entity2076 is generally higher than that of @entity1881 . However, @entity1881 plasma levels are about four fold higher than those of @entity2076 . Among other factors, a preferential cellular uptake of @entity2076 over @entity1881 could contribute to the observed higher plasma @entity1881 levels. In this investigation, we studied the uptake and depletion of both @entity1881 and @entity2076 (separately and together) in cultured RAW 264.7 macrophages. Similar studies were performed with @entity5629 and @entity5629 , which are oxidation products of @entity4793 . RESULTS: RAW 264.7 macrophages showed a greater uptake of @entity2076 compared to @entity1881 (with uptake being defined as the net difference between @entity4793 transported into the cells and loss due to catabolism and/or in vitro oxidation). Surprisingly, we also found that the presence of @entity2076 promoted the cellular uptake of @entity1881 . Mass balance considerations suggest that products other than @entity3276 were formed during the incubation of @entity4793 with macrophages. CONCLUSION: Our data suggests that @entity2076 could play a significant role in modulating intracellular antioxidant defence mechanisms. Moreover, we found the presence of @entity2076 dramatically influenced the cellular accumulation of @entity1881 , i.e., @entity2076 promoted the accumulation of @entity1881 . If these results could be extrapolated to in vivo conditions they suggest that @entity2076 is selectively taken up by cells and removed from plasma more rapidly than @entity1881 . This could, in part, contribute to the selective maintenance of @entity1881 in plasma compared to @entity2076 .
