Tubular complexes in XXXX - and @entity3952 -induced @entity788 in @entity35 : glycoconjugate pattern, immunocytochemical, and ultrastructural findings.

@entity9112 @entity788 @entity8689 @entity3952 @entity660 @entity3210 @entity35 @entity8629 @entity10811
Ductlike tubular complexes in @entity8629 -induced @entity788 and @entity3952 -induced @entity3210 were studied to analyze further their origin and development. Immunocytochemistry for pancreatic enzymes, lectin-binding studies, and ultrastructural investigations were combined with autoradiographic quantitation of labeling indices of ductlike cells in tubular complexes. In one group of @entity35 , @entity788 was induced by infusion of @entity8629 (10 micrograms kg-1 h-1). In a second group, @entity3210 was induced by intraductal injection of @entity3952 (50 microliters). The investigations were carried out at distinct intervals following induction of @entity660 . In both groups of animals, after 3 days, a significant widening of acinar lumina was paralleled by a decreasing height of acinar cells, which showed pronounced retrogressive changes. At this time, acinar cells bound all of the lectins used and retained their immunoreactivity for @entity9112 . At further intervals, acinar structures formed typical ductlike complexes, with a progressive loss of immunoreactivity for pancreatic enzymes and a reduced lectin-binding for @entity8689 and @entity10811 . Autoradiographic quantitation demonstrated no significant labeling of acinar cells undergoing tubular dedifferentiation. In both models, tubular complexes were removed by macrophages. It is concluded that lining cells in tubular complexes represent degenerating acinar cells that have no regenerative potency and have lost their secretory and membrane characteristics.
