Moderate Exercise Increases Affinity of Large Very Low-Density Lipoproteins for Hydrolysis by XXXX .

@entity1 @entity26 @entity412 @entity5523 @entity65
CONTEXT: Postprandial @entity412 (TG) concentration is independently associated with @entity65 risk. Exercise reduces postprandial TG concentrations, but the mechanisms responsible are unclear. OBJECTIVE: The objective was to determine the effects of exercise on affinity of chylomicrons, large very low-density lipoproteins (VLDL1), and smaller VLDL (VLDL2) for @entity5523 ( @entity5523 )-mediated TG hydrolysis. DESIGN: This was designed as a within- @entity1 crossover study. SETTING: The setting was a university metabolic investigation unit. @entity1 : @entity1 were 10 overweight/obese @entity1 . INTERVENTIONS: @entity1 undertook two oral fat tolerance tests, separated by 7-14 days, in which they had blood taken while fasting and for 4 hours after a high-fat mixed meal. On the afternoon before one test, they performed a 90-minute treadmill walk at 50% maximal @entity26 uptake (exercise trial [EX]); no exercise was performed before the control trial (CON). MAIN OUTCOME MEASURES: We measured circulating TG-rich lipoprotein concentrations and affinity of chylomicrons, VLDL1, and VLDL2 for @entity5523 -mediated TG hydrolysis. RESULTS: Exercise significantly reduced fasting VLDL1-TG concentration (CON, 0.49 [0.33-0.72] mmol.L(-1); EX, 0.36 [0.22-0.59] mmol.L(-1); geometric means [95% confidence interval]; P = .04). Time-averaged postprandial chylomicron-TG (CON, 0.55    0.10 mmol.L(-1); EX, 0.39    0.08 mmol.L(-1); mean    SEM; P = .03) and VLDL1-TG (CON, 0.85    0.13 mmol.L(-1); EX, 0.66    0.10 mmol.L(-1); P = .01) concentrations were both lower in EX than CON. Affinity of VLDL1 for @entity5523 -mediated TG hydrolysis increased by 2.2 (1.3-3.7)-fold [geometric mean (95% confidence interval)] (P = .02) in the fasted state and 2.6 (1.8-2.6)-fold (P = .001) postprandially. Affinity of chylomicrons and VLDL2 was not significantly different between trials. CONCLUSIONS: Exercise increases affinity of VLDL1 for @entity5523 -mediated TG hydrolysis both fasting and postprandially. This mechanism is likely to contribute to the TG-lowering effect of exercise.
