Inflammatory microenvironment and expression of XXXX .

@entity12835 @entity19 @entity5 @entity635 @entity15891 @entity72063 @entity9701 @entity842 @entity35028 @entity157
AIM: To study the inflammatory microenvironment and expression of @entity157 ( @entity157 ) in @entity19 . METHODS: CBRH-7919 @entity157 cells were injected into the subcutaneous region of @entity19 . Beginning two weeks after the challenge, @entity5 growth was measured every week for six weeks. The stromal microenvironment and inflammatory cell infiltration was assessed by immunohistochemistry in paired @entity5 and adjacent peritumoral samples, and macrophage phenotype was assessed using double-stain immunohistochemistry incorporating expression of an intracellular enzyme. A chemokine PCR array, comprised of 98 genes, was used to screen differential gene expressions, which were validated by Western blotting. Additionally, expression of identified chemokines was knocked-down by RNA interference, and the effect on @entity5 growth was assessed. RESULTS: Inflammatory cell infiltrates are a key feature of adjacent peritumoral tissues with increased macrophage, neutrophil, and T cell (specifically helper and activated subsets) infiltration. Macrophages within adjacent peritumoral tissues express inducible @entity842 synthase, suggestive of a proinflammatory phenotype. Fifty-one genes were identified in @entity5 tissues during the progression period, including 50 that were overexpressed (including @entity15891 , @entity9701 and @entity72063 ) and three that were underexpressed ( @entity35028 , Ifg and @entity12835 ). RNA interference of @entity15891 in the CBRH-7919 cells decreased the growth of @entity5 in @entity19 and inhibited expression of @entity9701 , @entity72063 and @entity635 protein. CONCLUSION: These findings suggest that @entity15891 plays a critical role in @entity5 growth and may serve as a potential molecular target for use in @entity157 therapy.
