Transfection of MS-36 XXXX cells with gef gene inhibits proliferation and induces modulation of the cell cycle.

@entity1522 @entity5 @entity10713 @entity619 @entity2823 @entity320 @entity526
The gef gene, found in @entity526 DNA, encodes a small (50 @entity1522 ) protein which is related to cell-killing functions. We used the MS-36 @entity320 cell line as an experimental model to examine the usefulness of the gef gene as a new strategy for @entity5 therapy. We transfected MS-36 cells using the pMAMneo vector, and induced gef gene expression with @entity619 . This decreased the proliferation rate of MS-36TG by as much as 85% in comparison with MS-36 parental cells. The decrease in cell growth was accompanied with significant modifications of the cell cycle and morphology. The G1-phase gradually disappeared, with accumulation in the S-phase. However, studies with annexin V- @entity2823 and @entity10713 failed to demonstrate induction of apoptosis. Morphological changes were an increase in cell size and the number of filopodia, and especially the appearance of pore-like alterations in the cell membrane which were not seen in parental cells. Our results demonstrate that the gef gene, a system independent of the administration of a prodrug, significantly reduces the proliferation of MS-36 cells. This gene may therefore be considered a new candidate for @entity5 gene therapy.
