Identification of XXXX / @entity39937 gene expression as a predictive biomarker of adipose tissue expansion sensitive to dietary anti- @entity28 interventions.

@entity5204 @entity28 @entity19 @entity36719 @entity71277 @entity3089 @entity3237 @entity39937 @entity4025 @entity1386
UNASSIGNED: Food components with anti- @entity28 properties are commonly evaluated using @entity19 models of diet-induced @entity28 . The ability of these components to reduce or prevent @entity1386 ( @entity1386 ) accumulation is usually tested in feeding trials of several weeks duration in order to detect significant effects on fat mass expansion. Here, we aimed to identify early, predictive biomarkers for @entity1386 expansion. We performed a 5-day high-fat diet (HFD) feeding trial with C57BL/6J @entity19 using different established anti- @entity28 interventions: @entity3089 , replacing dietary lipids by n-3 @entity5204 , and increasing dietary protein. @entity1386 gene expression was analyzed of genes known to be similarly affected by short- and long-term HFD. Gene expression of @entity71277 (mesoderm-specific transcript) was increased by HFD and normalized by all anti- @entity28 interventions. In a second experiment, translatability to whole blood-based expression data was assessed. @entity19 were challenged for 21  days with a HFD without or with simultaneous treatment with anti- @entity28 bioactives, @entity36719 or @entity4025 , and compared for parameters including Leptin and @entity39937 expression in whole blood at day 5. While @entity3237 mRNA could not be detected in @entity19 whole blood, there was an induction of @entity39937 mRNA by HFD which was suppressed by @entity36719 . Moreover, @entity39937 expression in whole blood at day 5 positively correlated with @entity1386 and negatively with lean body mass and the subcutaneous/visceral fat ratio at day 21. We conclude that gene expression of @entity3237 and @entity39937 in @entity1386 and of @entity39937 in whole blood represent early, predictive markers of adipose tissue expansion of potential usefulness in nutritional studies and trials.
