Initiation of phospholipomannan b-1,2 mannosylation involves Bmts with redundant activity, influences its cell wall location and regulates b-glucans homeostasis but is dispensable for @entity5132 XXXX .

@entity1 @entity71567 @entity71569 @entity71566 @entity71568 @entity5132 @entity281 @entity1200 @entity364 @entity375
UNASSIGNED: Pathogenic and non-pathogenic fungi synthesize glycosphingolipids, which have a crucial role in growth and viability. Glycosphingolipids also contribute to fungal-associated pathogenesis. The opportunistic @entity375 pathogen @entity5132 synthesizes phospholipomannan (PLM), which is a glycosphingolipid of the @entity71566 family. Through its lipid and glycan moieties, PLM contributes to the initial recognition of the @entity375 , causing @entity1200 and persistent @entity364 through activation of host signaling pathways. The lipid moiety of PLM activates the deregulation signaling pathway involved in @entity375 phagocytosis whereas its glycan moiety, composed of b-1,2 @entity71567 (b- @entity1 ), participates to inflammatory processes through a mechanism involving Galectin-3. Biosynthesis of PLM b- @entity1 involves two b-1,2 mannosyltransferases (Bmts) that initiate ( @entity71568 ) and elongate (Bmt6) the glycan chains. After generation of double bmts   mutants, we show that @entity71568 has redundant activity with @entity71569 , which can replace @entity71568 in @entity71568   mutant. We also report that PLM is located in the inner layer of the @entity375 cell wall. PLM seems to be not essential for @entity281 of the @entity375 . However, defect of PLM b-mannosylation increases resistance of @entity5132 to inhibitors of b-glucans and chitin synthesis, highlighting a role of PLM in cell wall homeostasis.
