Molecular Characterization of the Intestinal Microbiota in XXXX With and Without Abdominal Bloating.

@entity1 @entity23 @entity1744
BACKGROUND: Recent studies have demonstrated differences in the intestinal microbiota between @entity1 with @entity1744 ( @entity1744 ) and @entity23 ( @entity23 ) suggesting a role for the intestinal microbiota in the pathogenesis of @entity1744 . Alterations in the microbiota have also been implicated in the pathogenesis of abdominal bloating, a commonly reported symptom in @entity1744 . We investigated the relationship between the intestinal microbiota, abdominal bloating and altered bowel patterns in a cohort of @entity1 with @entity1744 and @entity23 . METHODS: The 16S rRNA gene from fresh fecal samples were amplified and pyrosequenced using Roche-454 Titanium chemistry. A Core Measurable Microbiome (CMM) was generated for Operational Taxonomic Unit (OTU) detected in >75% of all samples and compositional features of CMM were compared between groups using Linear Discriminant Analysis (LDA). KEY RESULTS: @entity1744 differentiated from @entity23 by LDA using continuous variation in the species/OTUs or the CMM genera. When subcategorized based on bloating symptoms and bowel characteristics, the same subjects were also well-differentiated from one another and from @entity23 . ANOVA analysis showed quantitative species/OTUs differences between the subgroups including @entity1744 with and without bloating, and subtypes based on bowel characteristics. CONCLUSIONS _ INFERENCES: The clear LDA differentiation and the significant microbial taxa differences between the groups imply a significant association of the microbiota with bloating symptoms and bowel characteristics in @entity1744 . These changes in the microbiota may serve as a biomarker for @entity1744 and its clinical subtypes and suggest a role for the intestinal microbiota in the pathogenesis of the main symptoms of the disorder.
