Expression of insulin-like growth factors and corresponding binding proteins (IGFBP 1-6) in XXXX spinal cord and peripheral nerve after axonal injuries.

@entity2620 @entity7815 @entity10680 @entity66 @entity10777 @entity1897 @entity2135 @entity10778 @entity35 @entity1930 @entity2619
Insulin-like growth factors (IGFs) exert trophic effects on several different cell types in the nervous system, including spinal motoneurons. After @entity1897 , the increased expression of IGFs in the damaged nerve has been suggested to facilitate axonal regeneration. Here we have examined the expression pattern of mRNAs encoding @entity10680 , IGF binding proteins (IGFBPs) 1-6 in the @entity35 spinal cord and peripheral nerve in three lesion models affecting lumbar motoneurons, i.e., sciatic nerve transection, ventral root avulsion, and a cut lesion in the ventral funiculus of the spinal cord. The expression was also studied in enriched Schwann cell and astrocyte cultures. The injured sciatic nerve expressed @entity2135 and @entity2620 as well as @entity10777 and @entity10778 , whereas central nervous system (CNS) scar tissue expressed @entity2135 , @entity7815 , and @entity10778 . @entity2619 mRNA was strongly upregulated in spinal motoneurons after all three types of lesions. @entity2619 -like immunoreactivity was present in motoneuron cell bodies, @entity1930 . In line with the in vivo findings, cultured Schwann cells expressed @entity2135 , @entity2620 , @entity10777 , and @entity10778 mRNAs, whereas cultured astrocytes expressed @entity2135 , @entity7815 , and @entity10778 mRNAs. These findings show that @entity2135 is available for lesioned motoneurons both after peripheral and central @entity66 , whereas there are clear differences in the expression patterns for @entity2620 and some of the binding proteins in CNS and peripheral nervous system (PNS) scar tissue. The robust upregulation of @entity2619 mRNA in lesioned motoneurons suggests that this binding protein may be of special relevance for the severed cells.
