Regulation of intrinsic apoptosis in @entity1880 -treated macrophages by the Sichuan XXXX of Chinese Leishmania isolates.

@entity1 @entity1357 @entity588 @entity1880 @entity19 @entity232 @entity281
UNASSIGNED: Leishmania spp. are able to survive and proliferate inside mammals' mononuclear phagocytes, causing @entity232 . Previous studies have noted that the regulation of apoptosis in host cells by these parasites may contribute to their ability to evade the immune system. However, current results remain unclear about whether the parasites can promote or delay the apoptotic process in host cells, because the regulatory effect of Leishmania was assumed to be strain-, species- and even @entity281 time-dependent. The aim of this study was to investigate whether the Sichuan isolates of Chinese Leishmania (SC10H2) can alter the process of intrinsic apoptosis induced by @entity1880 in different types of macrophage cell lines and to determine in which steps of the signaling pathway the parasites were involved. @entity1 @entity1357 and @entity19 RAW264.7 macrophages were infected by SC10H2 promastigotes followed by @entity1880 stimulation to assess the alteration of intrinsic apoptosis in these cells. The results indicated that SC10H2 @entity281 of @entity1 @entity1357 macrophages could promote the initiation of intrinsic apoptosis, but completely opposite results were found in @entity19 RAW264.7 macrophages. Nevertheless, the expression of @entity588 and the DNA fragmentation rates were not altered by SC10H2 @entity281 in the cell lines used in the experiments. This study suggests that SC10H2 promastigote @entity281 is able to promote and delay the transduction of early apoptotic signals induced by @entity1880 in @entity1357 and RAW264.7 macrophages, revealing that the regulation of intrinsic apoptosis in host cells by SC10H2 in vitro occurs in a host cell-dependent manner. The data from this study might play a significant role in further understanding the relationship between Leishmania and different host cells.
