An experimental model of coronary XXXX without suturing.

@entity548 @entity301 @entity235 @entity254 @entity583
OBJECTIVE: The aim of the study is to explore the feasibility and mid-term patency of an easier anastomotic technique for @entity301 ( @entity301 ). METHODS: Eight mongrel @entity235 (+/-15 kg) underwent direct @entity254 between the left internal thoracic artery (LITA) and the left anterior descending coronary artery (LAD) via inferior sternotomy on the beating heart. After positioning the graft, the distal part of the LAD was opened to allow retrograde filling of the LITA-graft. The @entity254 was secured by the use of biological glue (BioGlue, Cryolife, Marietta, GA, USA). No intravascular suture material was used. Ischemic time averaged 6 min. The proximal LAD was occluded upstream the arteriotomy. All survivors were angiographically controlled for patency after 6-8 weeks. Consequently, four @entity235 were sacrificed after 6 weeks and the remaining after 3 months for anatomo-pathological and histological examination by light and electron microscopy of the anastomotic site. RESULTS: All procedures were successful except for one animal that died of uncontrollable @entity548 at the anastomotic site. Another sustained post-operative transmural anterior @entity583 due to a late graft occlusion. All angiographically controlled grafts were patent with two vascular strings near the anastomotic site. Histology showed early macrophage infiltration into the glue. At post-mortem examination, new endothelialization was noticed in 80% of the cases. However, ultrastructural examination detected marked differences in endothelial fibroblastic lining compared to normal histology. CONCLUSIONS: Good mid-term permeability of the LITA grafts was observed in this new anastomotic technique for @entity301 in the @entity235 model. Although neo-endothelialization was present in most cases, ultrastructural differences were noticed after 3 months in the neo-intima compared to normal.
