Characterization of XXXX Genotypes in China--Identification of a New Polymorphism @entity71532 in the 16S rRNA Gene.

@entity1 @entity6475 @entity71532 @entity4430 @entity1201
@entity1201 continues to be prevalent in some mountainous regions of China, and genotypes of @entity6475 endemic to the country are not known. @entity4430 lepromatosis is a new species that was discovered in Mexico in 2008, and it remains unclear whether this species exists in China. Here, we conducted PCR- restriction fragment length polymorphism (RFLP) analysis to classify genotypes of 85 DNA samples collected from @entity1 from 18 different provinces. All 171 DNA samples from skin biopsies of @entity1201 @entity1 were tested for the presence of @entity4430 and @entity4430 lepromatosis by amplifying the 16S rRNA gene using nested PCR, followed by DNA sequencing. The new species M. lepromatosis was not found among the 171 specimens from @entity1201 @entity1 in 22 provinces in China. However, we found three SNP genotypes among 85 @entity1201 @entity1 . A mutation at @entity71532 in the 16S rRNA gene was found in 76% of the strains. We also found that the strains that showed the 16S rRNA @entity71532 mutation belonged to SNP type 3, whereas strains without the point mutation belonged to SNP type 1. The SNP type 3 @entity6475 were observed in @entity1 from both the inner and coastal regions of China, but the SNP type 1 strains were focused only in the coastal region. This indicated that the SNP type 3 @entity6475 were more prevalent than the SNP type 1 strains in China. In addition, the 16S rRNA gene sequence mutation at @entity71532 also indicated a difference in the geographical distribution of the strains. To our knowledge, this is the first report of a new polymorphism in 16S rRNA gene in @entity4430 in China. Our findings shed light on the prevalent genotypes and provide insight about @entity1201 transmission that are important for @entity1201 control in China.
