Independent overexpression of the subunits of translation elongation factor complex eEF1H in XXXX @entity2 .

@entity1 @entity5 @entity1149 @entity2 @entity1093 @entity14624
BACKGROUND: The constituents of stable multiprotein complexes are known to dissociate from the complex to play independent regulatory roles. The components of translation elongation complex eEF1H ( @entity14624 , eEF1Ba, eEF1Bb, eEF1By) were found overexpressed in different @entity5 . To gain the knowledge about novel @entity5 -related translational mechanisms we intended to reveal whether eEF1H exists as a single unit or independent subunits in different @entity1 @entity5 . METHODS: The changes in the expression level of every subunit of eEF1H in the @entity1 @entity1149 tissues were examined. The localization of eEF1H subunits was assessed by immunohistochemistry methods, subcellular fractionation and confocal microscopy. The possibility of the interaction between the subunits was estimated by co-immunoprecipitation. RESULTS: The level of eEF1Bb expression was increased more than two-fold in 36%, @entity14624 By in 28%, @entity14624 in 20% and @entity14624 Ba in 8% of @entity5 specimens. The @entity5 -induced alterations in the subunits level were found to be uncoordinated, therefore the increase in the level of at least one subunit of eEF1H was observed in 52% of samples. Nuclear localization of eEF1Bb in the @entity5 rather than distal normal looking tissues was found. In @entity5 tissue, @entity14624 and eEF1Ba were not found in nuclei while all four subunits of @entity14624 H demonstrated both cytoplasmic and nuclear appearance in the @entity2 cell line A549. Unexpectedly, in the A549 nuclear fraction @entity14624 lost the ability to interact with the @entity1093 complex. CONCLUSIONS: The results suggest independent functioning of some fraction of the eEF1H subunits in @entity1 @entity5 . The absence of @entity14624 and @entity1093 interplay in nuclei of A549 cells is a first evidence for non-translational role of nuclear-localized subunits of @entity1093 . We conclude the appearance of the individual @entity1093 subunits in @entity5 is a more general phenomenon than appreciated before and thus is a novel signal of @entity5 -related changes in translation apparatus.
