The regulatory influence of prostaglandins on protein synthesis in the XXXX non pregnant cervix.

@entity1 @entity1522 @entity535 @entity1421 @entity3157 @entity134 @entity720 @entity3209 @entity1521
Cervical connective tissue was obtained from non pregnant fertile @entity1 undergoing hysterectomy. Tissue specimens were mechanically chopped into 1 mm thick slices which were preincubated in Krebs-Ringer @entity3157 buffer containing @entity1421 or PGF2 alpha (300 ng/ml). After 60 min the slices were transferred into fresh buffer with the corresponding PG-concentration and 3H-labelled @entity1521 or @entity720 . Following incubation (60 min) the protein bound radioactivity was determined and related either to the dry weight or to the protein content of each slice. The two methods used did not show any qualitative differences. The experiments showed that PG:s had a marked effect on protein synthesis in the cervical tissue. During the follicular phase incubation with both types of PG:s was followed by a decreased incorporation indicating decreased net synthesis of collagen while there was an increased incorporation and hence increased synthesis in the luteal phase. There was no significant influence on the distribution of the model @entity535 @entity3209 -AIB in the presence of PG:s neither in the follicular nor in the luteal phase. The present data point to an acute effect of @entity1421 and PGF2 alpha on cervical collagen metabolism and indicate furthermore that the process is @entity134 hormone dependent. It is concluded that these substances may exert their effect by modulating the incorporation of @entity1522 into protein.
