Ploidy and morphology in XXXX .

@entity8339 @entity75 @entity5 @entity421 @entity3
In a cytometric DNA study of high-grade @entity421 , the relationship between DNA content and morphology was analyzed. The investigation, based on microspectrophotometry of tissue sections and flow cytometry (FCM), included both @entity75 and recurrences. FCM analysis, applied to a consecutive series of 47 primary @entity421 , disclosed that 2 were diploid and 45 were nondiploid, 8 of which were @entity8339 . Multiple aneuploid peaks were detected in 13 @entity5 . Among the @entity5 , there was no clear relationship between the peak DNA value(s) and the histologic subtype (osteoblastic, chondroblastic, fibroblastic) or grade (III-IV). The proliferative activity, as reflected by the percentage of S-phase cells, could be determined in 38 of the 47 @entity5 analyzed by FCM. The percentage was higher for aneuploid than for @entity8339 lesions; however, the distribution of S-phase cells was not related to the histologic subtype or the grade of the @entity5 . To assess the reliability of a single sample for FCM, the DNA content of biopsy and surgical specimens was compared in 20 @entity5 ; there was complete agreement in all cases with respect to the classification of the lesion as diploid, @entity8339 or aneuploid. Analysis by FCM or microspectrophotometry of 12 local recurrences and 16 @entity3 and the corresponding 19 @entity5 showed that an aneuploid characteristic of the @entity75 was retained during progression of the disease. In 12 @entity5 analyzed by microspectrophotometry in tissue sections, comparison of chondroblastic and osteoblastic/fibroblastic areas within the same lesion consistently disclosed hyperploidy in both areas.(ABSTRACT TRUNCATED AT 250 WORDS)
