Characterisation of lymphocyte subpopulations in XXXX .

@entity11529 @entity7571 @entity2225 @entity1195 @entity5399 @entity15989 @entity16997 @entity2280
AIMS: Interstitial @entity1195 + cells and T lymphocytes have previously been demonstrated within @entity11529 ( @entity11529 ). This study investigated the expression of B and T lymphocyte markers by the @entity1195 + population, and the expression of @entity5399 , a marker of thymocyte progenitors, which have the ability to give rise to both B and T cells. METHODS: Immunohistochemical (IHC) staining was performed on proliferating and involuted IHs for the expression of @entity1195 , CD3, @entity2225 , @entity7571 , @entity5399 and @entity2280 . The presence of mRNA corresponding to @entity1195 , @entity16997 , @entity2225 and @entity5399 was confirmed by reverse transcriptase-polymerase chain reaction in snap-frozen @entity11529 tissues. Cell counting of @entity15989 IHC-stained slides was performed on @entity1195 + only cells and dually stained @entity1195 +/CD3+ cells or @entity1195 +/ @entity2225 + cells and analysed statistically. In situ hybridisation and mass spectrometry were also performed to confirm the presence and abundance of @entity5399 , respectively. RESULTS: IHC staining showed a subpopulation of @entity1195 + interstitial cells that expressed the T lymphocyte marker, CD3, and another subpopulation that expressed the B lymphocyte marker, @entity2225 , in proliferating and diminished in involuted IHs. The abundant expression of @entity5399 on the endothelium of proliferating, but not involuted @entity11529 , was demonstrated by IHC staining and confirmed by in situ hybridisation and mass spectrometry. CONCLUSIONS: Both B and T lymphocytes are present within the interstitium of proliferating and involuted @entity11529 . The expression of @entity5399 by the endothelium suggests that B and T cells in @entity11529 may have originated from within the lesion, rather than migrating from the peripheral circulation.
