Evaluation of the RIM-N, Gonochek II, and Phadebact systems for the identification of pathogenic Neisseria spp. and XXXX .

@entity6368 @entity1735 @entity11791 @entity6729 @entity3631
Methods for identifying Neisseria spp. include conventional and modified @entity1735 degradation procedures, chromogenic enzyme substrate tests, and immunologic coagglutination tests for @entity6368 . In this study, we evaluated the abilities of the RIM-N @entity1735 degradation system (American MicroScan, Campbell, Calif.), the Gonochek II enzymatic identification system (Du Pont Co., Wilmington, Del.), and the Phadebact Gonococcus coagglutination test (Pharmacia Diagnostics, Piscataway, N.J.) to identify pathogenic Neisseria spp. and @entity6729 . Both stock strains and clinical isolates, including 176 @entity6368 , 173 @entity3631 , 48 @entity11791 , and 12 @entity6729 strains, were tested. The RIM-N identified 98% of the gonococci, 99% of the meningococci, 94% of the @entity11791 strains, and 100% of the @entity6729 strains within 1 h. The Gonochek II system identified 99% of the gonococci, 97% of the meningococci, 100% of the @entity11791 strains, and 100% of the @entity6729 strains within 30 min. Phadebact coagglutination provided clearly positive results for only 77% of the @entity6368 strains, producing negative or equivocal results with 23% of the strains. The RIM-N and Gonocheck II tests generally produced clear-cut reactions. An additional advantage of the Gonocheck II system was the small inoculum required for the performance of the test compared with the other systems, thus allowing the identification of @entity6368 directly from the primary isolation medium.
