Quantitative functional MRI in a clinical orthotopic model of @entity788 in immunocompetent Lewis XXXX .

@entity788 @entity6098 @entity82 @entity5 @entity3 @entity35 @entity420
OBJECTIVE: To demonstrate feasibility of performing quantitative MRI measurements in an immuno-competent @entity35 model of @entity788 by comparing in vivo anatomic and quantitative imaging measurements to @entity5 dissemination observations and histologic assays at necropsy. Meterials and methods: @entity35 ductal pancreatic @entity420 @entity35 cell line and Lewis @entity35 were used for these studies. 10(8) @entity35 cells were injected subcutaneously into the right flank of donor @entity35 . Donor @entity5 reaching 10 mm were excised, and 1 mm(3) @entity5 fragments were implanted within recipient @entity35 pancreas during mini-laparotomy. T1-weighted, T2-weighted, diffusion-weighted, and dynamic contrast-enhanced (DCE) MRI were performed using a Bruker 7.0T ClinScan. After MRI, all animals underwent autopsy. @entity5 size was measured, and dissemination score was used to assess local invasion and distant @entity3 . @entity5 and all sites of @entity3 were harvested and fixed for H_E, Masson's trichrome, and rat anti- @entity6098 staining. Trichrome slides were scanned and digitized for measurement of fibrotic tissue areas. Anti- @entity6098 slides were used for microvessel density (MVD) measurements. RESULTS: @entity5 , local invasion, and distant @entity3 were confirmed for all @entity35 . No significant differences were found between in vivo MRI measurements (48.7    5.3 mm) and ex vivo caliper measurements (43.6    3.6 mm) of @entity5 sizes (p > .05). Spleen, liver, diaphragm, peritoneum, and abdominal wall @entity3 were observed on MRI but smaller lung, mediastinum, omen, and mesentery @entity3 were only observed at necropsy. Contrast uptake observed during DCE measurements was significantly greater in both primary and metastatic @entity5 tissues compared to skeletal muscle and normal liver tissues. Both primary and @entity3 were hyper-intense in T2-weighted images and hypo-intense in T1-weighted images, but no differences were found between quantitative T2 measurements in @entity5 and that in @entity3 . Similarly, quantitative ADC measurements were similar for both @entity5 and liver @entity3 (1.13    0.3 * 10(-3) and 1.24    0.4 * 10(-3) mm(2)/s, respectively). Histologic @entity82 and MVD measurements were similar in @entity5 and @entity3 . CONCLUSIONS: Anatomic and quantitative functional MRI measurements are feasible in orthotropic DSL @entity35 model and will permit non-invasive monitoring of @entity5 responses during longitudinal studies intended to develop new interventional therapies for primary and metastatic disease.
