Molecular fingerprint of neuropeptide S-producing neurons in the XXXX brain.

@entity4889 @entity19 @entity9830 @entity148 @entity24068 @entity9829
@entity4889 ( @entity4889 ) has been associated with a number of complex brain functions, including @entity148 -like behaviors, arousal, sleep-wakefulness regulation, drug-seeking behaviors, and learning and memory. In order to better understand how @entity4889 influences these functions in a neuronal network context, it is critical to identify transmitter systems that control @entity4889 release and transmitters that are co-released with @entity4889 . For this purpose, we generated several lines of @entity19 that express enhanced green-fluorescent protein (EGFP) under control of the endogenous @entity4889 precursor promoter. @entity4889 /EGFP- @entity19 show anatomically correct and overlapping expression of both @entity4889 and EGFP. A total number of    500 @entity4889 /EGFP-positive neurons are present in the @entity19 brain, located in the pericoerulear region and the K  lliker-Fuse nucleus. @entity4889 and transgene expression is first detectable around E14, indicating a potential role for @entity4889 in brain development. EGFP-positive cells were harvested by laser-capture microdissection, and mRNA was extracted for expression profiling by using microarray analysis. @entity4889 was found co-localized with galanin in the K  lliker-Fuse nucleus of the lateral parabrachial area. A dense network of orexin/ @entity24068 neuronal projections contacting pericoerulear @entity4889 -producing neurons was observed by immunostaining. Expression of a distinct repertoire of metabotropic and ionotropic receptor genes was identified in both @entity4889 neuronal clusters that will allow for detailed investigations of incoming neurotransmission, controlling neuronal activity of @entity4889 -producing neurons. Stress-induced functional activation of @entity4889 -producing neurons was detected by staining for the @entity9829 gene @entity9830 , thus supporting earlier findings that @entity4889 might be part of the brain stress response network.
