Expression of the inducible form of XXXX synthase by reactive astrocytes after transient global @entity63 .

@entity2806 @entity63 @entity1506 @entity1249 @entity5219 @entity1110 @entity842 @entity40 @entity35 @entity528
We recently demonstrated that reactive astrocytes express @entity1110 diaphorase activity, a marker for @entity842 Synthase, following transient global @entity63 (Neuroscience Letters 154: 125-128). There has been little evidence that astrocytes express @entity842 Synthase or produce NO ( @entity842 ) in vivo; although in vitro experiments have shown that cultured astrocytes can produce NO. To determine whether reactive astrocytes express inducible form of NOS ( @entity1506 ) in vivo, we studied the pathological changes of @entity35 hippocampus by immunohistochemistry after 10 minutes of transient global @entity63 , which results in the selective delayed death of @entity1249 pyramidal cells and marked @entity5219 in the @entity1249 subfield. In the normal hippocampus, astrocytes express neither @entity1110 diaphorase activity nor @entity1506 . After @entity63 , the temporal and spatial pattern of @entity1506 , @entity1110 diaphorase, and @entity40 are very similar, indicating that reactive astrocytes express @entity1506 . Double staining for @entity1110 diaphorase and @entity40 , or @entity1506 and @entity40 confirmed that reactive astrocytes express both @entity1110 diaphorase activity and @entity1506 immunoreactivity. These changes were observed three day after @entity63 and increased in prominence from one week to one month. The staining pattern of OX42, an antibody that recognizes both microglia and macrophages, is spatially and temporally distinct from the pattern of @entity1110 diaphorase and @entity1506 staining. Thus, we conclude that transient global @entity63 induces @entity1506 primarily in reactive astrocytes. This increase in NOS expression and, presumably, NO production by reactive astrocytes may play a role in the process of delayed @entity528 or in the remodeling responses that occur after @entity2806 .
