Scanning electron microscopic observations on the interaction between normal neuronal and XXXX cells in monolayer culture.

@entity5 @entity1573 @entity816 @entity521
Interaction of embryonic @entity1573 cerebral cells with the @entity816 (C6) and @entity521 (N2a) cells were examined by SEM. When @entity816 cells were added to the glial monolayer cell substratum the glial substratum was loosened and @entity816 cells grew into the gaps of the loosened glial substratum. It is believed that the penetrating property of the @entity816 cells may be related to the invasiveness of the @entity5 cells. When @entity521 (N2a) cells were added to normal glial substratum, no invasion of the glial substratum was observed. Instead, for the first 2 days the @entity5 cells rested on the glial substratum and sent out numerous fine filopodia (0.1-0.2 micrometers) closely adhered to the surface of the glial cells. On the third day, the groups of glial cells underneath the @entity521 cells, which have been covered by filopodia of the @entity5 cells were rounded and became retracted from the glial substratum. These morphologically altered cells showed features of the @entity5 cells. It is therefore speculated that @entity521 cells have the ability of changing (transforming?) the glial cells probably through their filopodia. When @entity521 cells were added to the @entity816 substratum, there was an initial reaction on both cell types in which lamellipodia were extremely developed. This initial reaction subsided about 24 hours later and the @entity521 cells became more flattened, and grew on the @entity816 substratum. In this case no rounding up of @entity816 cells was observed. Normal neuronal cells when added to @entity816 substratum, showed well differentiated neuronal characteristics. This indicates that neuronal differentiation can be maintained by a @entity5 substratum.
