Study of Collagen Birefringence in Different Grades of XXXX Using Picrosirius Red and Polarized Light Microscopy.

@entity5 @entity957
UNASSIGNED: Objectives. The present study was done to evaluate birefringence pattern of collagen fibres in different grades of @entity957 using Picrosirius red stain and polarization microscopy and to determine if there is a change in collagen fibres between different grades of @entity957 . Materials and Methods. Picrosirius red stained 5    m thick sections of previously diagnosed different grades of @entity957 and normal oral mucosa were studied under polarization microscopy for arrangement as well as birefringence of collagen fibres around @entity5 islands. Results. It was found that thin collagen fibres increased and thick collagen fibres decreased with dedifferentiation of OSCC (P < 0.0001). It was observed that there was change in polarization colours of thick fibres from yellowish orange to greenish yellow with dedifferentiation of OSCC indicating loosely packed fibres (P < 0.0001). Conclusion. There was a gradual change of birefringence of collagen from yellowish orange to greenish yellow from well to poorly differentiated @entity957 , indicating that there is a change from mature form of collagen to immature form as @entity5 progresses. Studying collagen fibres with Picrosirius red for stromal changes around @entity5 islands along with routine staining may help in predicting the prognosis of @entity5 .
