Hormonal response of primary hepatocytes of the clawed toad, XXXX .

@entity268 @entity1238 @entity1425 @entity10063 @entity413
Freshly isolated hepatocytes of the clawed toad, @entity1238 , were cultured for at least 3 days. The viability of the cells was characterized using staining and biochemical methods. In particular, the @entity413 and glycogen balance was tested. After culture for 16-20 hrs, the cells were subjected to hormonal treatment. Both @entity1425 and @entity10063 stimulated the release of @entity413 in a dose dependent manner. 10(-6) M concentrations were strongly effective. The determination of the glycogen balance made it clear that the @entity413 release is mainly due to glycogenolysis. Using receptor antagonists and agonists, it has been shown that the effect of @entity1425 is clearly mediated by beta-type receptors. @entity10063 stimulated glycogenolysis via a type of receptor which is similar to the V2-receptor of mammals. This means that @entity268 is involved in the response to both types of hormones which is in contrast to that which is known about the effect of nonapeptides on the liver of mammals.
