XXXX protects against apoptosis in neuronal cell line caused by @entity2426 -induced depletion of intracellular calcium stores.

@entity11329 @entity137 @entity2426 @entity21808 @entity19 @entity1855 @entity4527 @entity1880 @entity460 @entity18650 @entity141 @entity1379 @entity4271
The @entity137 of @entity2426 , a selective inhibitor of endoplasmic reticular @entity141 -ATPase, was investigated in GT1-7 cells, a @entity19 hypothalamic cell line. Treatment of these cells with 50 or 100 nM @entity2426 greatly reduced cell viability at 24 and 48 h. These doses of @entity2426 induced a rapid rise in free cytosolic @entity141 ([ @entity141 ]i), followed by a sustained increase. Addition of EGTA to chelate extracellular @entity141 diminished somewhat the size of the initial increase of [ @entity141 ]i caused by @entity2426 , and abolished the sustained increase. The sustained increase could also be abolished by addition of @entity11329 and by @entity21808 , a drug selective for receptor-mediated @entity460 entry, but not by @entity1855 or @entity4271 . Pretreatment with 50 microM BAPTA/AM, a cytosolic @entity141 chelator, inhibited the peak [ @entity141 ]i caused by @entity2426 but did not inhibit the sustained elevation of [ @entity141 ]i. Neither @entity4527 nor @entity18650 /AM inhibited the cell death induced by @entity2426 . The cell death was characterized by DNA fragmentation ("laddering"), nuclear condensation and fragmentation, and was inhibited by protein synthesis inhibitor @entity1880 , all characteristic of apoptotic cell death. Overexpression of the protooncogene @entity1379 in GT1-7 cells inhibited significantly DNA fragmentation, nuclear condensation and fragmentation, and cell death induced by @entity2426 . However, @entity1379 did not alter either basal [ @entity141 ]i or the elevation of [ @entity141 ]i induced by @entity2426 . Our results suggest that abnormal @entity141 release from endoplasmic reticulum caused by @entity2426 induces GT1-7 death by apoptosis and that this effect does not depend on @entity141 influx from the extracellular space. @entity1379 inhibited apoptosis induced by @entity2426 , but the mechanism is unlikely to be inhibition of endoplasmic reticular @entity141 release in GT1-7 neuronal cells.
