[Effects of XXXX promotion on proliferation and osteogenic differentiation of @entity1 periodontal ligament stem cells].

@entity1 @entity19 @entity622 @entity692 @entity98 @entity2454 @entity6455 @entity460 @entity623 @entity24527
OBJECTIVE: To evaluate the effects of @entity24527 ( @entity6455 ) on the proliferation and osteogenic differentiation of @entity1 periodontal ligament stem cells (hPDLSCs) in vitro and in vivo. METHODS: An enzymatic digestion block was used in vitro to culture hPDLSCs, which were separated and purified by limited dilution cloning. The hPDLSCs were identified using cell-surface markers and cocultured with 1 x 10(-7) mol.L-1 @entity6455 solution. The proliferation ability of these cells was determined by @entity2454 ( @entity98 ) assay. After staining with @entity623 ( @entity623 ), osteogenesis was detected by enzyme-linked immunosorbent assay. Osteoblast-related genes were analyzed by reverse transcription-polymerase chain reaction. @entity622 staining was performed to measure the level of @entity460 deposition. The hPDLSCs were cocultured with 1 x 10(-7) mol.L-1 @entity6455 and nano- @entity692 scaffolds in vivo before transplantation into subcutaneous tissues of @entity19 . Osteogenic abilities were histochemically analyzed after 30 days of induction. RESULTS: The hPDLSCs were affected by 1 x 10(-7) mol.L-1 @entity6455 , and @entity98 assay showed that the proliferation of the groups treated with @entity6455 in vitro was better than that of the control groups on the second day. The @entity623 activity of the treated hPDLSCs was significantly enhanced after cell culture for 3, 5, and 7 days. The gene expression of osteoblastic markers was also significantly enhanced after 7 days. The deposition of mineralization after incubation with 1 x 10(-7) mol.L-1 @entity6455 increased compared with the control after cell culture for 14, 21, and 28 days. Furthermore, the bone expression of the treatment groups in vivo was significantly enhanced compared with that of the control groups. CONCLUSION: Treatment with 1 x 10(-7) mol.L-1 @entity6455 can significantly promote proliferation and differentiation of hPDLSCs in vitro and in vivo. @entity6455 can effectively function as a bioactive growth factor in periodontal tissue engineering to replace traditional growth factors.
