Video time-lapse microscopy of XXXX @entity189 in vitro.

@entity1 @entity5 @entity189 @entity10086
Twelve @entity1 @entity189 and 14 normal vocal cord epithelia were studied in vitro by the method of tissue culture incorporating video time-lapse microscopy. Different events were photographed by taking 'stills' from the television monitor during play-back. Outgrowths from both @entity5 and normal explants formed only as monolayers. Mitoses were mainly found to be localized approximately mid-way between explant and the edge of the outgrowth. Some cells were seen to become suddenly active producing oscillating blebs and sometimes they detached themselves from the monolayer culture and changed appearance. This was more prevalent in the @entity5 cultures. In both @entity5 and normal cell cultures lymphocytes were observed and their normal chemokinetic movement was changed into a rapid chemotactic attraction by the presence of a mitotic epithelial cell. These lymphocytes appear to represent T-suppressor cells. The present study has shown that primary tissue culture can be a valuable tool in the study of @entity189 . The interaction between mitotic @entity5 cells and lymphocytes may represent a transformation of T-lymphocytes into @entity10086 cells.
