Penile neuronal XXXX synthase and its regulatory proteins are present in hypothalamic and spinal cord regions involved in the control of penile erection.

@entity398 @entity1024 @entity1131 @entity6806 @entity2821 @entity842 @entity546
Control of penile erection requires the coordination of the hypothalamus and the L6-S1 region of the spinal cord. Erection requires the activation of neuronal @entity842 synthase ( @entity2821 ), which is tightly regulated. Because variants of @entity2821 (penile @entity2821 : PnNOS) and the @entity1024 receptor (truncated NMDAR subunit 1: @entity6806 -T) as well as @entity1131 ( @entity1131 ) have all been located in the @entity546 , this work aims to determine whether these proteins are also present in the hypothalamus. It was found that PnNOS, the brain-type @entity2821 , and @entity1131 , were expressed in the hypothalamus. In contrast, @entity6806 -T was expressed only in the penis, whereas the brain-type @entity6806 was present in the brain and sacral spinal cord and not in the penis. PnNOS was found in the media preoptic area, posterior magnocellular, and the parvocellular regions of the paraventricular nucleus, supraoptic nucleus, septohypothalamic nucleus, medial septum, cortex, and in some of the @entity2821 staining neurons throughout the brain. It was absent in the organum vasculosum of the lamina terminalis. @entity1131 staining was present in neurons of the medial preoptic area, paraventricular nucleus, medial septum, and cortex, but not in the supraoptic nucleus, septohypothalamic nucleus, or organum vasculosum of the lamina terminalis. Colocalization between PnNOS and @entity1131 was found in the medial preoptic area, medial septum, and cortex, and less in the paraventricular nucleus. PnNOS and @entity398 were colocalized in the paraventricular nucleus and supraoptic nucleus. In hypothalamic extracts, recombinant @entity1131 -GST protein bound to PnNOS in the extracts and partially inhibited NOS activity. These results indicate that both @entity2821 variants, and their respective regulatory proteins are present and colocalize in the hypothalamic and spinal cord regions involved in penile erection.
