Isolation of a novel polyomavirus, related to XXXX endothelial cell-infecting virus, from marbled eels, @entity3813 (Quoy _ Gaimard).

@entity3813 @entity548 @entity16932 @entity450 @entity281
UNASSIGNED: Marbled eels, @entity3813 (Quoy _ Gaimard), cultured in Taiwan exhibited @entity548 and mortality in January 2012. The severely diseased eels bled from the gills and showed @entity450 filaments and @entity548 throughout the body similar to viral endothelial cell necrosis of eel. In this study, a novel polyomavirus (AmPyV) was isolated from the diseased eels using the AMPF cell line established from the pectoral fin of healthy marbled eels. AmPyV was found to encode a long T-antigen orthologous gene. Phylogenetic analysis showed that AmPyV was closely related to @entity16932 endothelial cell-infecting virus. PCR assays revealed @entity281 throughout the systemic organs. AmPyV proliferated in the AMPF, EK-1 and EO-2 cells at temperatures 25-30    C, and the progeny virus yields were 10(7.0) , 10(7.4) and 10(7.7) TCID50   mL(-1) , respectively. The purified virions were icosahedral particles, 70-80  nm in diameter. No clinical signs or mortality was observed among the eels injected with the virus; however, the virus was reisolated from the brain, eyes, kidneys, fins and gills of infected eels 2  month after injection. Our results suggest that AmPyV exhibits a @entity281 . Pathogen of the disease needs to study further.
