Adhesion molecules on normotensive and XXXX @entity35 brain endothelial cells.

@entity64 @entity3692 @entity101 @entity39 @entity5 @entity35 @entity731 @entity1590
The intercellular adhesion of circulating leukocytes to vascular endothelium is a prerequisite for leukocyte emigration from the blood to extravascular tissues. This process is facilitated by adhesion molecules on the surfaces of both the vascular endothelial cells and the leukocytes. The experiments presented here demonstrate for the first time that the leukocyte adhesion receptor, @entity1590 , is constitutively expressed on cultured cerebromicrovascular endothelial cell lines derived from both spontaneously @entity101 (SHR) @entity35 and normotensive @entity35 -Kyoto (WKY) @entity35 . Both cultures contained similar numbers of cells constitutively expressing this adhesion molecule (31.4% and 29.6%, respectively). Adhesion molecule expression was up-regulated by interleukin-1 beta, @entity5 @entity39 factor-alpha, @entity3692 and lipopolysaccharide in a dose- and time-dependent manner. Both cultures exhibited similar maximum levels of adhesion molecule up-regulation to optimal concentrations of all three cytokines. However, SHR endothelial cells were more sensitive to all three cytokines; significantly higher levels of @entity1590 expression were seen on SHR as opposed to WKY endothelial cells cultured with sub-optimal cytokine concentrations. It was also observed that lipopolysaccharide up-regulated @entity1590 expression on SHR endothelial cells to a greater extent than on WKY endothelial cells. The findings that @entity1590 can be up-regulated to a greater degree on SHR endothelial cells may have important implications for in vivo perivascular leukocyte accumulation under @entity101 conditions. These observations indicate a possible mechanism by which @entity101 may predispose to the development of disorders such as @entity731 and @entity64 .
