Effects of intracolonic opioid receptor agonists on polymodal pelvic nerve afferent fibers in the XXXX .

@entity35 @entity13847 @entity3575 @entity14
We studied the effects of intracolonic administration of opioid receptor agonists (ORAs) on responses of pelvic nerve afferent fibers to @entity14 ( @entity14 ) and heat. Single-fiber recordings were made from the decentralized S1 dorsal rootlet in the @entity35 . An approximately 7-cm length of descending colon was isolated in situ to permit intracolonic perfusion with Krebs solution, which, when the outflow was clamped, was used to distend the colon. Responses to noxious @entity14 (40 mmHg, 30 s) were tested after intracolonic instillation of mu-, delta- or kappa-ORAs. Intracolonic administration of the kappa-ORAs EMD 61,753 (n = 5/12) and U62,066 (n = 8/11), but not either the mu-ORA @entity3575 or the delta-ORA @entity13847 , concentration-dependently inhibited responses of afferent fibers. For fibers unaffected by intracolonic administration of EMD 61,753 or U62,066, intra-arterial administration of kappa-ORAs was effective. Forty-one of 54 mechanosensitive fibers also responded to intracolonic instillation of heated Krebs solution (50 degrees C). Intra-arterial injection of @entity3575 or @entity13847 did not attenuate responses to heat. Either intracolonic or intra-arterial administration of EMD 61,753 or U62, 066, however, inhibited afferent fiber responses to heat. These results document that mechanical and thermal sensitivity of polymodal pelvic nerve afferent fibers innervating the @entity35 colon can be inhibited peripherally by intracolonic instillation of kappa-ORAs.
