Cellular thermotolerance is associated with XXXX protein 70.1 genetic polymorphisms in Holstein lactating @entity291 .

@entity1457 @entity377 @entity72363 @entity72361 @entity515 @entity13285 @entity72364 @entity1422 @entity291 @entity72362
@entity1457 proteins (Hsp) are known to protect cells from several stressors. Nucleotide changes in the flanking regions [5'- and 3'-untranslated region (UTR)] of Hsp gene might affect inducibility, degree of expression, or stability of Hsp70 mRNA. The present study aimed to investigate the association between inducible @entity72361 single nucleotide polymorphisms (SNPs) and @entity1457 ( @entity1457 ) response of peripheral blood mononuclear cells (PBMC) in dairy @entity291 . Four hundred forty-six Italian Holstein @entity291 were genotyped for four @entity72361 SNPs: g895 C/- and g @entity72362 in 5'-UTR, and g @entity72363 and @entity72364 in 3'-UTR. Genetic polymorphisms in 3'-UTR of @entity291 @entity72361 gene resulted monomorphic. Distribution of alleles of the @entity377 sequence polymorphism within the 5'-UTR of the @entity291 @entity72361 gene were 81.2% and 18.8% for C and -, respectively, and 77.8% and 22.2% for G and T, respectively. Among the 446 genotyped animals, a group of @entity291 balanced for days in milk and parity was selected to be representative of the following genotypes: CC (n   =   8), C- (n   =   7), and -- (n   =   7) and GG (n   =   8), @entity1422 (n   =   11), and TT (n   =   3) in 5'-UTR. PBMC were isolated from blood samples and heated at 43  C in thermal bath for 1  h and then incubated at 39  C in atmosphere of 5% @entity515 for 1, 2, 4, 8, 16, and 24  h (recovery times). Cell viability was determined by XTT assay. Gene and protein expression of @entity72361 was determined by real-time reverse transcription-polymerase chain reaction and by ELISA assay, respectively. For the two SNPs detected, one allele was the most frequent (C, 66.8% and G, 56.8%). Genotypes -- and TG showed higher (P   <   0.05) viability compared with CC and GG, respectively. Genotypes C- and TT had intermediate viability. Gene expression of @entity72361 showed higher (P   <   0.001) levels in -- and TG genotype compared with their counterparts. Genotypes -- and TG showed the higher level of inducible @entity72361 protein in respect to C-, TT and CC, GG. In conclusion, exposure to @entity1457 differently affected cell viability and gene and protein expression of @entity72361 in the selected genotypes. These results indicate that the presence of SNPs (C/- and @entity13285 ) in the 5'-UTR region of inducible @entity72361 ameliorates @entity1457 response and tolerance to heat of @entity291 PBMC. These mutation sites may be useful as molecular genetic markers to assist selection for heat tolerance.
