Determination of XXXX in Cordyceps sinensis and Ganoderma lucidum by high performance liquid chromatography method.

@entity2642 @entity859 @entity1042 @entity2441 @entity5536 @entity47525 @entity1883 @entity1718 @entity68
BACKGROUND: @entity2642 are supportive in the regulation and modulation of various physiological processes in body, they acts as precursors in nucleic acid synthesis, enhance immune response, help in absorption of @entity68 and influence the metabolism of @entity1718 . Cordyceps sinensis and Ganoderma lucidum are well-known for its use in traditional medicine of China, Nepal and India. They are rich in @entity2642 such as @entity5536 , @entity859 , @entity1883 , etc. Hence, a simple, economic and accurate high-performance liquid chromatography (HPLC) analytical method was proposed for determination of @entity5536 and @entity859 for the quality control of plants. MATERIALS AND METHODS: Chromatographic experiments were conducted on YL9100 HPLC system (South Korea). Reversed-phase chromatography was performed on a C18 column with @entity1042 and @entity47525 as the mobile phase in isocratic elution method at a flow rate of 1.0 mL/min. Detection was carried out at 254 nm, which gives a sharp peak of @entity5536 and @entity859 at a retention time of 6.53    0.02 min and 12.41    0.02, respectively. RESULTS AND DISCUSSION: Linear regression analysis data for the calibration plot showed a good linear relationship between response and concentration in the range of 25-200   g/mL for @entity859 and 100-800   g/mL for @entity5536 with regression coefficient of 0.999 and 0.996, respectively. The @entity5536 was found 0.16% and 0.71% w/w in G. lucidum and in @entity2441 , respectively, and @entity859 was found to be 0.14% w/w in G. lucidum whereas absent in @entity2441 . CONCLUSION: The developed HPLC method for the quantification of @entity859 and @entity5536 can be used for the quality control and standardization of crude drug and for the different herbal formulations, in which @entity5536 and @entity859 are present as major constituents. The wide linearity range, sensitivity, accuracy, and simple mobile phase imply the method is suitable for routine quantification of @entity859 and @entity5536 with high precision and accuracy.
