Anticalmodulin autoantibody in XXXX : a new antibody against a cytoskeleton-related protein.

@entity1 @entity2835 @entity409 @entity1252 @entity1575 @entity3619 @entity99 @entity291
An ELISA has been developed for detection of auto-antibodies against calmodulin. There was a significantly increased frequency (63.1%) of autoantibodies against calmodulin in 103 @entity1 with chronic @entity409 as compared to that (30%) of @entity1 with @entity1252 and to that (6.9%) of normal subjects (p less than 0.01). IgG autoantibodies against calmodulin were detected in the @entity1 with acute @entity99 (37.9%), @entity409 (45.6%) and also in the @entity1 with @entity1252 (30%). IgM autoantibodies against calmodulin were frequently found in @entity1 with @entity1575 (52.2%), @entity2835 (50%) and @entity3619 (38.7%), but rarely in @entity1 with acute @entity99 (13.8%), @entity3619 (9.5%) and @entity1252 (0%). IgA autoantibodies against calmodulin were frequently found in @entity1575 (33.3%), @entity2835 (42.9%) and @entity3619 (53.6%), but rarely in @entity3619 (15.8%), @entity3619 (14.3%) and @entity1252 (0%). The occurrences of autoantibodies against calmodulin correlated neither with those of antismooth muscle antibody, antinuclear antibody and antimitochondrial antibody, nor with serum IgG concentrations. Autoantibodies against calmodulin did not cross-react with troponin, myosin light chain, @entity291 thymus DNA and actin. The titer of autoantibodies against calmodulin was decreased by absorption of serum with calmodulin and the liver plasma membrane fraction. The immunoblotting experiment revealed the binding of autoantibodies against calmodulin to calmodulin. IgG fraction from a @entity1 with @entity3619 inhibited the activation of phosphodiesterase by calmodulin.(ABSTRACT TRUNCATED AT 250 WORDS)
