Glycosaminoglycan production by @entity19 XXXX variants in vivo and in vitro.

@entity17540 @entity19 @entity5 @entity4023 @entity3 @entity48401 @entity320 @entity597
Glycosaminoglycan (GAG) synthesis of B-16 @entity320 metastatic variants was examined in vivo and in vitro to begin to assess the relationship between the presence of these polymers and the process of primary invasion and @entity3 . The variants that were examined for GAG production included the F-1 line that exhibits low metastatic potential, the @entity48401 line selected for high metastatic potential, and the BL6 line selected for high invasiveness. The F-1 cell line was routinely less invasive than the @entity48401 and BL6 lines when injected s.c. into the legs of irradiated Swiss Webster @entity19 . All cell lines formed palpable @entity5 after s.c. injection, but histological sections revealed early and extensive invasion in only @entity48401 and BL6 @entity5 . The F-1 @entity5 were surrounded by a connective tissue capsule and did not begin to invade into host tissue until this structure disappeared approximately 16 days after injection of @entity5 cells. Some consistent alterations in GAG synthesis, particularly the release of hyaluronic acid and heparan @entity597 , were observed among the cell lines in vivo and in vitro, although differences observed in vitro were small and variable. In vivo all @entity5 were surrounded by a hyaluronic acid-rich zone that was concentrated at the @entity5 -stromal interface and was transitory. @entity17540 occurred as a diffuse band around BL6 and @entity48401 @entity5 but was confined to a capsule surrounding the less aggressive F-1 @entity5 . In vitro the BL6 and @entity48401 cell lines released larger amounts of heparan @entity597 and @entity4023 than did the F-1 cell line. Differences in release of chondroitin @entity597 by the cell lines were not observed. Differences in trypsin-releasable GAG, presumably associated with the glycocalyx, were also not apparent. These results link the release in vitro and organization in vivo of hyaluronic acid and heparan @entity597 to invasion and @entity3 .
