Simultaneous expression of multiple immune complex receptors on XXXX thymocytes and spleen cells.

@entity821 @entity19 @entity941 @entity6689 @entity3414 @entity6433
Mixed rosette studies were performed to evaluate the coexpression of IgG Fc. IgM Fc, and complement receptors (C3R) by thymocytes obtained from @entity19 7 days after @entity821 injection and by spleen cells. Indicator cells coated with IgM, IgG, or C3 independently were mixed and could be distinguished by morphology or by a @entity941 label. In double-marker studies, 36% of spleen cells formed rosettes with IgG- and/or IgM-sensitized red blood cells. Among this population there was a 24% overlap of cells binding IgM and IgG complexes simultaneously. Of the spleen cells, 78% bound IgM- and/or C3-sensitized cells. Of the spleen cells forming rosettes with IgM and C3 indicator cells, 15% coexpressed these receptors. With IgG and C3 indicator cells, 58% of spleen cells bound to one or both kinds of complexes with an 18% overlap. Of @entity821 -resistant thymocytes, 14% formed rosettes with IgM- and/or IgG-sensitized red blood cells; within this population there was an overlap of 21%. With IgM- or C3-sensitized cells, 19% of @entity821 -resistant thymocytes bound to one or both, among which there was a coexpression of 21%. With IgG- or C3-sensitized cells, there was a 14% overlap of rosette-forming cells binding both. In triple-marker studies 79% of spleen cells formed rosettes with C3-, IgG-, and/or IgM-sensitized indicator cells, out of which 11% coexpressed IgM and IgG @entity6433 , 20% coexpressed IgG and C3R, and 10% coexpressed IgM @entity6433 and C3R. Of rosette-forming cells, 13% coexpressed all three receptors. With @entity821 -resistant thymocytes, 19% bound one or more kinds of immune complexes. Among these, 9% coexpressed IgG @entity6433 and C3R, 14% coexpressed IgM @entity6433 and C3R, and 14% bound IgG and IgM complexes. We could not detect the simultaneous expression of all three receptors on @entity821 -resistant thymocytes. Using Isopaque-Ficoll fractionation of cells binding C3-sensitized cells, @entity821 -resistant thymocytes were enriched and depleted of C3-receptor-bearing cells and their Lyt phenotypes were determined by immunofluorescence microscopy. The C3-receptor-enriched population contained 56% C3R+ cells which were 79% @entity6689 positive and 100% @entity3414 positive. The C3R-depleted population contained 1.3% C3R+ cells with 10% @entity6689 positive and 22% @entity3414 positive among the total. Surface phenotypic expression of normal and @entity821 -resistant thymocytes was also evaluated by direct and indirect fluorescence by fluorescence-activated cell sorter (FACS).(ABSTRACT TRUNCATED AT 400 WORDS)
