Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related XXXX .

@entity14231 @entity12830 @entity19 @entity30266 @entity18501 @entity1939 @entity2005 @entity1886 @entity7002 @entity5231 @entity8024 @entity1020 @entity52434 @entity7112 @entity8387 @entity22416
Purpose: @entity1020 ( @entity1020 ), the most common cause of incurable @entity1939 in the western world, is characterized by the dysfunction and eventual death of choroidal endothelial (CECs), RPE, and photoreceptor cells. Stem cell-based treatment strategies designed to replace photoreceptor and RPE cells currently are a major scientific focus. However, the success of these approaches likely also will require replacement of the underlying, supportive choroidal vasculature. The purpose of this study was to generate stem cell-derived CECs to develop efficient differentiation and transplantation protocols. Methods: Dermal fibroblasts from the @entity1886 -GFP @entity19 were isolated and reprogrammed into two independent induced pluripotent stem cell (iPSC) lines via viral transduction of the transcription factors @entity7002 , @entity14231 , @entity2005 , and c-Myc. @entity1886 -GFP iPSCs were differentiated into CECs using a coculture method with either the RF6A CEC line or primary @entity19 CECs. Induced pluripotent stem cell-derived CECs were characterized via RT-PCR and immunocytochemistry for EC- and CEC-specific markers. Results: Induced pluripotent stem cells generated from @entity19 expressing green fluorescent protein (GFP) under control of the endothelial @entity1886 promoter display classic pluripotency markers and stem cell morphology. Induced pluripotent stem cell-derived CECs express @entity30266 , eNOS, @entity18501 , @entity12830 , @entity5231 , @entity22416 , @entity8024 , @entity1886 , @entity52434 , @entity7112 , and @entity8387 . Conclusions: Induced pluripotent stem cell-derived CECs will be a valuable tool for modeling of choriocapillaris-specific insults in @entity1020 and for use in future choroidal endothelial cell replacement approaches.
