[The influence of lentivirus-miRNA-184 on epithelial-mesenchcymal transition of XXXX lens epithelial cells in vitro].

@entity1 @entity5962 @entity1970 @entity2277 @entity6474
OBJECTIVE: To analyze the influence of miRNA-184 on epithelial-mesenchymal transition (EMT) of @entity1 lens epithelial cells (HLEC) induced by @entity6474 in vitro. METHODS: Experimental study. Recombinant plasmid of pL/IRES/GFP-miR-184 was constructed and used to produce the lentivirus. The lentivirus was used to transduce the HLEC which was in the process of EMT induced by @entity6474 ( @entity6474 ). The real-time fluorescence quantitative PCR (QRT-PCR) was used to analyze @entity5962 ( @entity5962 ), @entity1970 ( @entity1970 ), @entity2277 ( @entity2277 ) expression at RNA levels during interval of 0h, 6h and 24h after transduction, in comparison with that of control group. Statistical analysis method was single factor variance analysis. RESULTS: The expression level of epithelial marker gene @entity5962 in the miRNA-184 transduced group maintains relatively stable during 24h interval, while it goes down in the control group. The expression level of mesenchymal cell marker gene @entity2277 , @entity1970 in the miRNA-184 transduced group maintain relatively stable, while it goes up in the control group. The results of statistical analysis showed a statistically significant difference between miRNA-184 transduced group and control group (P < 0.05). CONCLUSION: miRNA-184 lentivirus-mediated HLEC can inhibit the occurrence of EMT.
