[Inhibition of angiogenesis in @entity3123 by XXXX antisense oligodeoxynucleotides].

@entity1 @entity3123 @entity1421 @entity189 @entity3009 @entity1573 @entity2247 @entity3840
OBJECTIVE: To investigate the effect of @entity3840 antisense oligodeoxynucleotides ( @entity2247 AS-ODNs) on the angiogenesis in @entity3123 and to evaluate the intermediary effect of @entity3009 2 in this process. METHODS: Specific targeting @entity2247 AS-ODNs were designed and synthesized, and transfected into the PC3 @entity1 @entity3123 cells cultured in vitro. Fluorescence microscopy was used to observe the PC3 cells 0.12. 24, 40, and 72 hours after the transfection. the second cultured PC3 cells were divided into 5 groups: control group, Lipo group (transfected with Lipofectin only), C1 group (transfected with 1 micro g @entity2247 AS-ODN + Lipo/well), C2 group (transfected with 2 micro g @entity2247 AS-ODN + Lipo/well), and C3 group (transfected with 3 micro g @entity2247 AS-ODN + Lipo/well). RT-PCR was used to observe the expression of @entity2247 mRNA in the PC3 cells. The third batch of PC3 cells were transfected with 3 micro g @entity2247 AS-ODN + Lipo/well, and the expression of @entity2247 mRNA was observed 0, 12, 24, 48, and 72 hours later by RT-PCR. 3 micro g @entity2247 AS-ODN + Lipo/bottle and 9 micro g @entity2247 AS-ODN + Lipo/bottle were added into the cultured PC3 cells and Western blotting was used to observe the expression of @entity2247 protein 24 hours later. 24 @entity1573 eggs were inoculated with PC3 cells into the chorio-allantoic membrane and then divided equally into 5 groups; control group, Lipo group, @entity2247 AS-ODN + Lipo group, and @entity2247 AS-ODN + Lipo + @entity1421 group. Leica microscopy was used to observe the angiogenesis in the transplanted @entity189 . RESULTS: RT-PCR showed that the downregulation of expression of @entity2247 mRNA in the PC3 cells with the increase of the @entity2247 AS-ODN concentration, peaking at the concentration of 0.2 micro mol/L. The effect of @entity2247 AS-ODN was strongest by the 12th hour after transfection and then began to decrease and basically disappeared 48 hours after. Western blotting showed that @entity2247 AS-ODN, especially that of the concentration of the expression of 9 micro g/bottle, inhibited the expression of @entity2247 AS-ODN. The angiogenesis of the transplanted @entity189 in the eggs was significantly inhibited in the Lipo + @entity2247 AS-ODN group, the density of newly generated vessels in the Lipo + @entity2247 AS-ODN + @entity1421 group was between those of the other 2 groups. CONCLUSION: @entity2247 AS-ODN significantly inhibits the angiogenesis in the @entity3123 . Endogenous @entity2247 AS-ODN may play an important role in such a process and @entity1421 may play an intermediate role therein.
