[Inosine pranobex - cytotoxic activities and effect of on replication of @entity1 @entity15609 viruses ( XXXX , @entity38545 ), entroviruses (CA16, EV71) and adenoviruses (HAdV-2, HAdV-5) in vitro].

@entity1 @entity778 @entity137 @entity14572 @entity15609 @entity1800 @entity19311 @entity98 @entity38545
INTRODUCTION: There are no specific antivirals designed for many @entity1800 . @entity14572 pranobex (PI) is a @entity19311 that is involved in a wide variety of intracellular biochemical processes. The mechanism of action in @entity1 body is still unclear but numerous studies have demonstrated that this drug inhibits viral replication and exhibit pleiotropic effect. We evaluated in vitro effect of @entity14572 pranobex (PI) on replication of @entity1 viruses: @entity15609 viruses ( @entity38545 , @entity38545 ), entroviruses A (CA16, EV71) and adenoviruses C (HAdV-2, HAdV-5). MATERIALS AND METHODS: In the present study, cytotoxic effect of @entity14572 pranobex was assessed using A549 cell line exposed to different concentrations of compound (PI: 50-800 ig/mL) for 48 hours. @entity137 effect of @entity14572 pranobex was assessed visually using light, inverted microscopy Olympus CK2 under 400x magnification and by the @entity98 colorimetric assay. Antiviral effect was estimated according to the reduction of virus titer. The yield reduction assay (YRA), which evaluates the ability of the PI (50-800   g/mL) to inhibit virus multiplication in cell cultures, was applied. The @entity778 was evaluated 48 h after infection ofA549 cell cultures with viruses by means of light, inverted microscopy. The Reed-Muench statistical method was used to determine the 50% end point (IC50) (yield reduction assay, YRA) in the presence of @entity14572 pranobex with the controlled one. RESULTS: There were no morphological changes, as assessed visually, in cell cultures treated with PI. @entity98 @entity137 assay confirmed microscopic observations. The viability of cells in the presence of the tested compounds was average 98, 36 %. After conducting the experiments and analyzing the results we noticed that higher concentrations of PI strongly inhibited multiplication of all viruses. PI weakly reduced the titer of infectious enteroviruses and @entity38545 as compared with the control. Adenoviruses showed the highest sensitivity to the antiviral activity of PI, however, increasing concentrations of PI up to 800   g /ml slightly enhanced the antiviral activity of 400   g/ml PI. CONCLUSIONS: Our study demonstrated that @entity14572 pranobex shows no cytotoxic activity on the A549 cell line. In conducted study was observed that adenoviruses (HAdV-2 and HAdV-5) and @entity38545 have the highest sensitivity to the antiviral activity of @entity14572 pranobex from all tested viral strains.
