Changes in expression and immunolocalization of protein associated with toxic @entity3645 -induced apoptosis in XXXX hepatocytes.

@entity10740 @entity1976 @entity4569 @entity2310 @entity1448 @entity35 @entity3645 @entity99
@entity99 results from the accumulation of toxic @entity3645 within the liver. The aim of the present study was to examine the temporal changes in expression and immunolocalization of protein associated with apoptosis in cholestatic @entity35 liver. @entity35 were anesthetized and @entity1448 was induced by double ligation of the common bile duct and sectioning between the ligatures. The animals were euthanized at day 3 and at weeks 1, 2, 4, and 6 after bile duct ligation (BDL). Apoptotic cell death was increased fivefold after 3 days of BDL, decreased over 2 weeks, and remained constant thereafter as has been demonstrated by TUNEL staining. Western blot analysis for @entity2310 , @entity1976 , cytochrome c, and @entity10740 were performed. Results show that total cellular @entity2310 protein was increased 3 days after BDL and decreased over time thereafter. We observed the translocation of @entity2310 to mitochondria and subsequent release of cytochrome c. According to our immunohistochemical data, nuclear @entity10740 increased 3 days after BDL, but cytoplasmic sequestration of @entity10740 was observed after 1 week. The expression of @entity4569 was inhibited by 3 days, but increased at later stages following BDL. @entity1976 was increased over time in BDL @entity35 . Our data suggest toxic @entity3645 -induced hepatocellular apoptosis is related to differential expression of @entity1976 family member protein and release of cytochrome c. Cellular localization of @entity10740 plays an important role in apoptotic death of hepatocytes in BDL @entity35 .
