Monoclonal antibody-defined phenotypes of regional lymph node and peripheral blood lymphocyte subpopulations in early XXXX .

@entity1 @entity0 @entity5 @entity189 @entity4755 @entity1196 @entity405
Lymphocyte subset phenotypes in peripheral blood and axillary lymph node cell isolated from 28 @entity1 undergoing surgery for @entity0 were determined by two-color immunofluorescence with monoclonal antibodies and flow cytometric analysis. Lymphocyte subpopulation proportions were determined with combinations of monoclonal antibodies directed against the @entity405 , Leu 3, @entity4755 , @entity1196 , Leu 11, Leu 12, Leu 15, Leu M3, and HLA-DR surface markers. @entity1 were staged according to the postsurgical-pathological modification of the Tumor-Node-Metastases staging system, for analysis of tissue source (lymph node versus peripheral blood) and stage of disease as factors influencing lymphocyte subset size. Activated @entity405 +DR+ and Leu 3+DR+T-cells were elevated in stage 2 @entity189 compared to Stage 1. Elevation of @entity405 +8+ circulating T-cells and a reciprocal depression of @entity405 +8- T-cells were also seen in Stage 2 @entity1 when compared to Stage 1. Total T-cells, B-cells, @entity405 +, and Leu 3+ T-cell subsets and natural killer phenotypes defined by @entity4755 and Leu 11 were unchanged in the peripheral blood of Stages 1 and 2 @entity0 . Regional lymph nodes from Stage 1 were found to contain a high frequency of Leu 3+ cells which dropped significantly in Stage 2 @entity1 ; this was found to be numerically due to a sharp decrease in the Leu 3+8- subpopulation in Stage 2 @entity1 . Elevated B-cells (Leu 12+), activated T-cells ( @entity405 +DR+ and Leu 3+DR+), total @entity405 + cells, and @entity4755 -11+ natural killer cells were demonstrated in Stage 2 lymph nodes when compared to Stage 1. Generally, no differences in subpopulations were seen when level 1 (low axillary) lymph node cells were compared to level 3 (high axillary) lymph node cells at each stage of the disease. These findings demonstrate substantial differences in the profile of lymphocyte phenotypes between Stage 1 and Stage 2 @entity0 , especially in the ipsilateral regional nodes. The findings presented in this study suggest that changes in local-regional immunocompetent cell subsets may be related to metastasis of @entity5 to the regional nodes and progression of disease without being fully reflected in the systemic circulation.
