Attempted isolation of a heparin proteoglycan from XXXX liver capsule.

@entity1522 @entity3363 @entity13488 @entity3649 @entity563 @entity4410 @entity291 @entity4337 @entity33291 @entity597
(1) @entity33291 were isolated from @entity291 liver capsule by extraction with 2m- @entity4337 followed by precipitation from 0.8m- @entity4337 with @entity4410 . @entity13488 was eliminated by digestion with hyaluronidase. The yield of heparin was approx. 40% of that obtained after extraction of the papain-digested tissue. (2) The macromolecular properties of the hyaluronidase-digested polysaccharide were studied by gel chromatography on Sephadex G-200 of the intact, as well as of the alkali-degraded, material. The results suggested the presence of single heparin chains in addition to a dermatan @entity597 proteoglycan. (3) A purified heparin preparation was analysed for @entity1522 and neutral @entity3363 . Xylose, @entity3649 and @entity563 were found in amounts corresponding to 0.1, 0.2, and 0.4 residue/polysaccharide chain (mol.wt. 7400), respectively. It is suggested that the isolated material had been degraded by a polysaccharidase with endo-enzyme properties.
