[Flowcytometric-immunophenotyping for XXXX needs to be standardized].

@entity706 @entity1195 @entity1778 @entity8625 @entity1583
Flowcytometer has been flourishing since the passed decade in our nation. Many general hospitals use it for routine tests. One of the main usage is immunophenotyping for @entity1583 . Flowcytometric immunophenotyping (FCM-IM) facilitates accurate diagnosis in both @entity1778 and @entity706 ( @entity706 ). It becomes an indispensable criteria in the diagnosis of @entity1583 . It may also offer additional therapeutically relevant information. Typical patterns of immunophenotype in @entity1778 and certain types of @entity706 are illustrated in figure 1 and figure 2. But variation or overlapping appears often. In order to reach a definitive diagnosis and to have a common interpretation between laboratory to laboratory the following items need not to be ignored: 1. Direct staining and multi-color or multi-parameter flow cytometric analysis is necessary. 2. The phenotype of abnormal cells should be determined on as pure a population as possible therefore @entity1195 /SSC gating is critical for isolating the blast population (abnormal cells). @entity1195 /SSC gating is much better than FSC/SSC gating especially when percentage of blast is low in the sample. Else, each @entity1778 has its special @entity1195 /SSC pattern. 3. The phenotype of this certain population should be described clearly but it is not necessary to count the percentage of each marker. 4. > 20% for positive threshold used in indirect staining method may be confused in multi-color direct staining method. 5. @entity8625 diagnosed by FCM only should be in caution since the divergence of antigen/antibody, the lineage infidelity of antigen expression and the biological characteristics of the leukemic cells. Flowcytometer is not a cell counter. "It provides a pattern of information that must be interpreted by a knowledgeable professional in the context of the clinical situation".
