Altered actin cytoskeletal patterns in two premalignant stages in @entity1 XXXX development.

@entity1 @entity652 @entity5 @entity189 @entity1412 @entity694 @entity47691
Primary culture of @entity1 colonic biopsies converts the single cell thick epithelial layer from a highly indented sheet in vivo into a flat patch on the surface of a Petri dish. Migration of cells from biopsies in a continuous sheet to form the patch cultures allows the cultured cells in large part to retain the junctional complexes and membrane interdigitations which connect adjacent cells in vivo and therefore to maintain their spatial relationships to neighboring cells. Migration of the cells onto a flat surface also allows visualization of their actin cables (E. Friedman, M. Verderame, S. Winawer, and R. @entity47691 , @entity5 Res., 44: 3040-3050, 1984). Actin organization patterns have been studied in primary patch cultures of colonic epithelial cells from four stages in the development of @entity694 : normal tissue, normal-appearing but preneoplastic cells characteristic of @entity1412 @entity1 , @entity5 or @entity652 from @entity1412 @entity1 , and benign and @entity5 from @entity1 in the general population. @entity189 exhibited the least number of actin cables, while @entity652 contained the greatest concentration. Similar actin patterns were seen in both @entity1412 and nonpolyposis @entity652 . The preneoplastic @entity5 stage characteristic of @entity1412 @entity1 had less actin cables than either normal cells or @entity5 cells. Thus actin organization loss characterized the transition from the normal colonic epithelial cell to the preneoplastic nontumor cell. The ability to form actin cables was then regained with the transition from the preneoplastic pretumor cell to the @entity5 cell and lost again with the @entity5 to @entity5 . The complexity of these changes in actin organization during the step-wise transformation of colonic epithelial cells was not predicted from the simple model of actin cable loss accompanying fibroblast transformation.
