@entity855 binding cassette transporter gene expression in XXXX liver progenitor cells.

@entity24072 @entity7359 @entity12311 @entity30167 @entity409 @entity32224 @entity21998 @entity855 @entity35 @entity24071 @entity18715 @entity5997 @entity72274
BACKGROUND AND AIM: @entity409 after severe @entity409 depends in part on proliferation and differentiation of hepatic progenitor cells (HPCs). Under these conditions they must be able to withstand the toxic milieu of the damaged liver. @entity855 binding cassette (ABC) transporters are cytoprotective efflux pumps that may contribute to the preservation of these cells. The aim of this study was to determine the ABC transporter phenotype of HPCs. METHODS: HPC activation was studied in @entity35 treated with @entity5997 ( @entity5997 ) followed by partial hepatectomy (PHx). ABC transporter gene expression was determined by real time detection reverse transcription-polymerase chain reaction in isolated HPCs, hepatocytes, cholangiocytes, and cultured progenitor cell-like RLF phi 13 cells and by immunohistochemistry of total liver samples. ABC transporter efflux activity was studied in RLF phi 13 cells by flow cytometry. RESULTS: 2-AAF/PHx treated animals showed increased hepatic mRNA levels of the genes encoding multidrug resistance proteins Mdr1b, @entity24071 , and @entity24072 . Immunohistochemistry demonstrated expression of @entity24071 and @entity24072 proteins in periportal progenitor cells and of the Mdr1b protein in periportal hepatocytes. Freshly isolated @entity18715 positive cells and cultured RLF phi 13 progenitor cells highly expressed @entity24071 and @entity24072 mRNA while the hepatocyte specific transporters @entity32224 , @entity30167 , @entity21998 , and @entity72274 were only minimally expressed. Blocking @entity24071 activity by @entity7359 resulted in accumulation of the @entity24071 specific substrate @entity12311 in RLF phi 13 cells. CONCLUSION: HPCs express high levels of active @entity24071 and @entity24072 . These may have a cytoprotective role in conditions of severe hepatotoxicity.
