Hepatic metabolism during acute @entity1068 administration: a @entity9715 nuclear magnetic resonance study on the perfused @entity35 liver under normoxic or XXXX conditions.

@entity6546 @entity9715 @entity26 @entity53200 @entity519 @entity35 @entity857 @entity1068 @entity855
The effect of @entity1068 metabolism on the energetic parameters and intracellular pH of the isolated perfused @entity35 liver from fed @entity35 was studied by @entity9715 nuclear magnetic resonance spectroscopy. This technique allowed us to analyze nondestructively and in real time the role of low @entity26 tension on the possible injurious effect of @entity1068 on the liver cells. A quantitative analysis of nuclear magnetic resonance data recorded on a perfused @entity35 liver within a 30 mm diameter probe has been performed at 80.9 MHz. Under normoxic and normothermic conditions, the levels of phosphorylated metabolites detected by nuclear magnetic resonance were 2.8, 0.3 and 2 mumoles per gm liver wet weight for @entity855 , @entity857 and inorganic @entity6546 , respectively. The cytosolic pH was 7.25 +/- 0.05. During a period of 4 min of @entity519 induced by reducing the perfusion flow rate to 25% of its initial value (i.e., from 12 ml to 3 ml per min per 100 gm body weight), the level of @entity855 dropped to 2.2 mumoles per gm liver wet weight. Concomitantly, @entity857 and inorganic @entity6546 increased to 0.6 and 3.3 mumoles per gm liver wet weight. Cytosolic pH fell to 7.02 +/- 0.05. Perfusion of the liver with a Krebs medium containing 70 mM (0.4%) @entity1068 induced a sharp decrease in intracellular inorganic @entity6546 to reach 1.3 mumole per gm liver wet weight and after a lag time of 4 to 6 min, a decrease in @entity855 level (2.15 mumoles per gm liver wet weight). A large increase in phosphomonoesters (mainly @entity53200 ) up to 6 mumoles per gm liver wet weight was also observed.(ABSTRACT TRUNCATED AT 250 WORDS)
