Effect of XXXX on smooth muscle cell functions.

@entity1 @entity64 @entity2426 @entity101 @entity4600 @entity1184 @entity141 @entity3281 @entity1808 @entity4601 @entity842 @entity3426 @entity35 @entity65
There is increasing evidence that fish oil-enriched diets attenuate the progression of several types of @entity1 and experimental renal, intestinal and @entity65 , including @entity101 . @entity4600 ( @entity4601 ), may be one of the active biological component. We previously reported that dietary @entity4601 suppressed the progression of @entity101 in @entity64 -prone spontaneously @entity101 @entity35 (SHRSP). The purpose of this study is to clarify the in vitro effect of @entity4601 on cultured smooth muscle cell functions such as cell growth, @entity1184 , NO release, and intracellular @entity141 metabolism, which are involved in the regulatory mechanisms of vascular tone. Addition of @entity4601 to the culture medium of aortic smooth muscle cells isolated from SHRSP and normotensive @entity35 Kyoto @entity35 (WKY) had no significant effects on cell growth or on cell @entity1184 induced by @entity1808 as measured by flow cytometry. @entity4601 had no stimulatory effect on @entity3426 (10 ng/ml)-induced @entity842 release from smooth muscle cells of SHRSP, but rather slightly inhibited it. However, the treatment of smooth muscle cells with @entity4601 (30 microM) for 2 days significantly suppressed the increase in intracellular @entity141 concentration induced by @entity1808 , but not by @entity2426 . This was due to the suppression of @entity141 influx, as determined by @entity3281 influx experiment. These results indicate that @entity4601 specifically suppresses @entity141 mobilization into smooth muscle cells. This may be one of the mechanisms by which dietary @entity4601 prevents the development of @entity101 in SHRSP.
