[Effects of microwave radiation on lens hydration and expression of XXXX and transcription factors in lens epithelial cells].

@entity53582 @entity20155 @entity2455 @entity1161 @entity95
OBJECTIVE: To observe the effects of low power microwave radiation on lens hydration and lens epithelial cells in vitro, and detect the expression of @entity53582 , c-fos and @entity20155 in lens epithelial cells. METHODS: @entity95 lens were exposed to microwave radiation with frequency of 2450 MHz and power density of 0.5, 2.0 and 5.0 mW/cm(2) in vitro. The hydration of lens was measured after 8 hours. Morphological changes of lens epithelial cells were observed using a phase-contrast microscope and @entity2455 staining. Expression of @entity53582 , c-fos and @entity20155 were analyzed using gel electrophoresis and western blot analysis. RESULTS: After 2.0 and 5.0 mW/cm(2) microwave radiation, the hydration of lens was increased compared to control groups (P<0.05), the shape of lens epithelial cells showed shrinking and disorder and cells nuclei appeared chromatin condensation. There was no change of lens and lens epithelial cells after 0.5 mW/cm(2) microwave radiation. The expression of @entity53582 was significantly increased in cell membrane, however, decreased in cell cytoplasm after 2.0 mW/cm(2) microwave radiation for 2, 4, 6 and 8 hours. There was significantly increased expression of c-fos and @entity20155 protein compared with control groups (P<0.05, P<0.01). CONCLUSION: Low power microwave radiation higher than 2.0 mW/cm(2) can activate @entity53582 by increasing its expression in cell membrane, then induce high expression of c-fos and @entity20155 , which may relate to cellular signaling pathway of microwave @entity1161 and lens epithelial cells.
