DNA flow cytometry as a prognostic indicator in XXXX .

@entity1 @entity135 @entity8339 @entity5 @entity957 @entity189 @entity1544 @entity982
The prognostic significance of deoxyribonucleic acid (DNA) flow cytometry has been investigated for many solid @entity5 , but few data have been accumulated for @entity957 of the head and neck. To our knowledge, we report the largest number of @entity1 (69) with @entity135 to be studied by DNA flow cytometry. In the first part of this study, we reviewed 109 consecutive @entity1 with laryngeal or hypopharyngeal primary @entity189 which were treated at North Carolina Memorial Hospital during the period of 1981 to 1984. The final analysis comprised 139 DNA histograms (mean coefficient of variation: 8.02) on @entity982 -embedded specimens from 48 @entity1 . Of the 48 @entity1 with primary @entity189 , 24 had glottic, 18 had supraglottic, and 6 had @entity189 from the piriform sinus. @entity1 had follow-up for a minimum of 12 months, with a mean follow-up period of 23 months. Twenty-three of the 48 primary @entity189 (48%) were clearly aneuploid, and the remaining 52% were tetraploid (22%) or diploid (30%). We have concluded that @entity1 with clearly aneuploid primary @entity189 had significantly better prognoses than those with @entity5 (p = 0.008). High DNA amounts (greater than 40% of cells beyond the diploid peak, DNA G1G0) also correlated with a favorable prognosis when compared with low DNA amounts (p less than 0.01), and this remained significant when the clinical outcome was adjusted for staging of the primary site (T), nodal status, and stage of disease. Ploidy was the most significant prognostic variable for the laryngeal group of @entity1 . In the second part of the study, twenty-one @entity1 with oral @entity1544 were studied in a similar fashion as the group with @entity189 . In this group, a low DNA amount, with 40% as the cutoff point, was associated with a favorable prognosis (p = 0.024), and this remained significant while controlling for T, nodal status, and stage of disease. Numbers were too small to permit evaluation of the impact of ploidy in this group, but there was a slight trend toward aneuploidy and @entity8339 , correlating with a poor treatment outcome (p = 0.228). DNA amount was the most significant prognostic variable for the group of @entity1 with oral @entity5 .(ABSTRACT TRUNCATED AT 400 WORDS)
