Influence of adrenoreceptor agonists and antagonists on ovulation in the XXXX ovary perfused in vitro.

@entity1 @entity1465 @entity5521 @entity95 @entity5333 @entity397 @entity1338 @entity3352 @entity1063 @entity4183
Ovaries from sexually mature virgin @entity95 were dissected free on both sides and cannulated in situ via that part of the aortic segment supplying the ovarian arteries. The ovaries were mounted in a closed, recirculating perfusion system for oxygenation in vitro. Ovulation was induced by @entity1 chorionic gonadotropin ( @entity1465 ) given either i.v. (100 IU) or directly into the perfusion medium (20 IU). @entity397 occurred in 42 out of a total of 54 ovaries. The in vivo route of @entity1465 administration was found to be most efficient, with ovulations occurring after a mean of 11.5 h, which corresponds well with the incidence of ovulations in situ. The prostaglandin synthesis inhibitor, @entity3352 , totally abolished the @entity1465 -induced ovulations. beta-Adrenoreceptor stimulation in vitro with @entity4183 or with @entity1063 in the presence of alpha-receptor blockade with @entity5333 caused a significantly increased number of gonadotropin-induced ovulations. @entity5521 or @entity5333 given alone reduced the number of ruptured follicles. @entity1063 (in the absence of @entity1465 ) and @entity1338 augmented the incidence of ovulation. The results indicate that local adrenoreceptor mechanisms participate in the process of ovulation, both via alpha-adrenoreceptors (probably affecting the follicular smooth musculature) and beta-adrenoreceptors (possibly through some trophic or endocrine mechanism other than a mechanical factor).
