[Expression of gap junction protein connexin 26 in human XXXX and its significance].

@entity82 @entity455 @entity3492 @entity5 @entity409 @entity3 @entity157 @entity99
UNASSIGNED: Objective:To investigate the expression of gap junction protein connexin 26( @entity3492 ) in @entity157 ( @entity157 ) and its significance. Methods:The expression of @entity3492 in liver tissue was examined by immunohistochemistry staining in 159 paraffin-embeded liver sections, including 20 samples of normal liver tissue, 30 samples of chronic @entity99 , 33 samples of liver @entity82 , and 76 samples of @entity157 . Normal hepatic cell line LO2 and @entity157 cell line SMMC-7721 were used in vitro to verify the characteristics of gap junction and @entity3492 expression pattern. The expression and localization of @entity3492 were measured by Western blotting and immunofluorescence assay, respectively. The function of gap junction between adjacent cells was detected by dye transfer assay. Results:Compared to normal liver samples, the positive rate of @entity3492 was markedly decreased in @entity99 , @entity82 and @entity157 tissues(all P<0.05). The intensity of @entity3492 staining was significantly increased in @entity157 tissues compared with those in @entity409 (NCL) tissues(all P<0.05). In NCL tissues, there was a mild to moderated staining of @entity3492 which located mainly on the membranes of hepatocytes at intercellular contacts. The positive staining of @entity3492 in @entity157 tissues was observed mainly in cytoplasm. Positive @entity3492 expression was positively associated with @entity5 size(P=0.036), but not with age, gender, histologic grade, clinical stage, underlying @entity99 and cirhosis, lymph node @entity3 and @entity455 (all P>0.05). Compared with LO2 cells, an aberrant expression and distribution of @entity3492 in SMMC-7721 cells was confirmed, which may lead to a decreased function of gap junctions. CONCLUSIONS: The aberrant expression and distribution of @entity3492 protein may be associated with hepatocarcinogenesis, and the residual gap junction in @entity157 may provide a new target for treatment of @entity157 .
