Immunohistochemical localization of two angiotensin I-converting isoenzymes in the reproductive tract of the male XXXX .

@entity32256 @entity95
The male reproductive tract contains two different isoenzymes of @entity32256 ( @entity32256 ), i.e., pulmonary and testicular @entity32256 . The present study shows selectively the cellular distribution of the @entity32256 isoenzymes in the reproductive tract of male @entity95 , using indirect immunofluorescence or immunoperoxidase methods. Testicular @entity32256 was found in the seminiferous tubules of the testes in spermatocytes containing mature spermatids, and in spermatids within the epididymal tubular lumen in sexually mature, but not in immature, @entity95 . Epididymal tubular cells contained pulmonary @entity32256 . In the young @entity95 , epididymal tissue contained more @entity32256 than that in adult @entity95 , since @entity32256 was observed in principal cells in addition to basal cells. In mature @entity95 , @entity32256 was observed in basal cells only. Strong staining for pulmonary @entity32256 was observed in cells of the vas deferens in both young and adult @entity95 . Therefore, synthesis of epididymal @entity32256 , unlike the testicular isoenzyme, was not stimulated by sexual maturation. Enzymatically active @entity32256 in seminal fluid corresponds to the pulmonary isoenzyme. The present study indicates that this seminal fluid @entity32256 may originate from cells of the epididymal tubules, particularly those of the vas deferens. Endothelial cells of blood vessels lying in the interstitium of both testicular and epididymal tissue contained the pulmonary isoenzyme.
