[Hypoglycemic effect of polysaccharide-coated insulin liposomes after oral administration in XXXX ].

@entity19 @entity14917 @entity8038 @entity413
AIM: To evaluate the hypoglycemic effect of chitosan-coated and @entity14917 -coated insulin liposomes after oral administration in @entity19 . METHODS: Insulin-liposomes were prepared by reverse-phase evaporation. @entity8038 was carried out by mixing liposomal suspension with chitosan and @entity14917 solutions, followed by incubation. The particle size and morphology of insulin-liposomes were determined using laser light scattering instrument and transmission electron microscopy (TEM). The entrapment efficiency was analyzed using HPLC and ultracentrifuge. The protection of insulin from peptic and tryptic digestion was studied with HPLC. The hypoglycemic effects of polysaccharide-coated insulin liposomes were investigated using the @entity413 oxidase method after oral administration in @entity19 . RESULTS: The particle size of uncoated, @entity8038 was (138 +/- 31) nm, (230 +/- 20) nm and (266 +/- 19) nm, respectively. All insulin-liposomes were of spherical or ellipsoidal shape. The entrapment efficiencies were 81.6%, 73.5% and 68.7%, respectively. Insulin was protected from tryptic digestion by chitosan-coated liposomes and protected from peptic digestion by alginate-coated liposomes. The hypoglycemic effects of insulin-liposomes, coated with 0.1% chitosan and 0.1% @entity14917 , were observed. CONCLUSION: Chitosan-coated and @entity14917 -coated liposomes were shown to reduce peptic or tryptic digestion on insulin, and enhance enteral absorption of insulin.
