@entity65661 is a phosphatidylinositol (3,4,5)-trisphosphate sensor that can bind the cytoskeletal adaptor, XXXX .

@entity8359 @entity2307 @entity2306 @entity19065 @entity65661 @entity17178
We identified a potential @entity19065 ( @entity19065 ) binding pleckstrin homology domain in the data bases and have cloned and expressed its full coding sequence ( @entity65661 ). The protein bound @entity19065 selectively in vitro. Strikingly, a substantial proportion of @entity65661 became associated with an unidentified intracellular vesicle population in the context of low @entity19065 levels produced by the addition of @entity2307 or @entity2306 . In addition, expression of platelet-derived growth factor-receptor mutants unable to activate type 1A @entity8359 ( @entity8359 ) or serum starvation in porcine aortic endothelial cells lead to redistribution of @entity65661 to this vesicle population. Importantly, pleckstrin homology domain mutants of @entity65661 that could not bind @entity19065 were constitutively localized to this vesicle population. At increased @entity19065 levels, @entity65661 was redirected to a predominantly cytoplasmic distribution, presumably through a @entity8359 -dependent block on its targeting to the vesicular compartment. Furthermore, at high, hormone-stimulated @entity19065 levels, it became significantly plasma-membrane localized. The distribution of @entity65661 is thus dramatically and uniquely sensitive to low levels of @entity19065 indicating it can act as a sensor of both low and hormone-stimulated levels of @entity19065 . In addition, @entity65661 bound to the cytoskeletal adaptor, @entity17178 , tightly and in a @entity8359 -independent fashion, both in vitro and in vivo. This interaction could co-localize heterologously expressed @entity17178 with GFP- @entity65661 in the unidentified vesicles.
