Metal-immobilized magnetic nanoparticles for cytochrome C purification from XXXX liver.

@entity71416 @entity8863 @entity35 @entity71415 @entity214
UNASSIGNED: @entity8863 -immobilized magnetic @entity71415 (mPHEMAH) nanoparticles were prepared by surfactant-free emulsion polymerization for cytochrome C (cyt C) purification from @entity35 liver. Elemental analysis, atomic force microscopy, zeta sizer, and vibrating sample magnetometer were used to characterize mPHEMAH nanoparticles. In addition to these characterization steps, surface area, average particle size, and size distribution of mPHEMAH nanoparticles were determined. Quantity of immobilized @entity8863 was measured using atomic absorption spectrophotometry. @entity71416 and @entity8863 content of mPHEMAH nanoparticles were 0.18 mmol/g polymer and 0.11 mmol/g polymer, respectively. Specific surface area of @entity8863 -immobilized mPHEMAH nanoparticles was 1180 m(2) /g. Effect of initial cyt C concentration, pH, temperature, and ionic strength on cyt C adsorption onto @entity8863 -immobilized mPHEMAH nanoparticles was investigated. Maximum cyt C adsorption capacity of @entity8863 -immobilized mPHEMAH nanoparticles was 311.9 mg/g polymer. Maximum adsorption was obtained at pH 8.0 and 4    C. @entity8863 -immobilized mPHEMAH nanoparticles were used ten times with 4.1% decrease in adsorption capacity. In the last stage, @entity8863 -immobilized mPHEMAH nanoparticles were used to purify cyt C from @entity35 liver tissue, and the purity of desorbed fractions was controlled by @entity214 -PAGE.
