Induction of @entity3334 gene transcription by @entity5 factor alpha via the @entity2451 in activated XXXX hepatic stellate cells.

@entity1 @entity3334 @entity2451 @entity5 @entity310 @entity99
BACKGROUND/AIMS: @entity99 results in the activation of hepatic stellate cells (HSCs), which in turn produce matrix metalloproteinase (MMP) in response to pro-inflammatory cytokines for tissue remodelling. This study explored the transcriptional induction of the @entity3334 gene by @entity5 factor-alpha ( @entity310 ) in HSCs. METHODS: The LI90 @entity1 HSC line was used in the present study. Gelatin zymography, enzyme-linked immunosorbent assay, Northern blotting and gene promoter-reporter assays were used to analyse the induction of @entity3334 protein, mRNA expression and gene transcription respectively. Deletional or site-directed mutations were introduced into the promoter region and transiently transfected into LI90 cells to determine the cis-acting elements necessary for @entity310 inducibility. Gel shift mobility assays were used to determine the transcriptional factors involved in the @entity310 responsiveness. RESULTS: @entity310 upregulated @entity3334 protein and mRNA expression in a dose-dependent manner. A time-course experiment revealed a rapid induction of @entity3334 mRNA expression after @entity310 treatment. Mutation in a putative nuclear factor (NF)-kappaB-binding site at -2541 bp almost completely abolished the @entity310 response to @entity3334 gene-promoter activity, suggesting transcriptional regulation of @entity3334 expression by @entity310 via this site. Electrophoretic mobility shift assay and supershift assays indicated that this transcriptional regulation was regulated via the @entity2451 of @entity2451 . CONCLUSIONS: @entity3334 gene expression might be induced by @entity310 via the @entity2451 of @entity2451 in activated @entity1 HSCs.
