XXXX ovary cells depend on exogenous lipids to survive phospholipase C treatment.

@entity137 @entity1718 @entity6579 @entity93 @entity2434 @entity1877 @entity13667 @entity2945 @entity3569 @entity165
@entity3569 ovary cells were maintained in culture medium supplemented with delipidated serum to make them dependent on nonlipid components for lipid synthesis. Growth in lipid-free medium resulted in an increased flux through the @entity13667 - @entity1877 pathway for @entity2434 synthesis. The increased flux appeared to be mediated by the @entity93 : @entity2945 cytidylyltransferase because cellular @entity2945 levels decreased in cells grown in lipid-free medium, and both cell-free cytidylyltransferase activity and membrane-associated cytidylyltransferase activity increased in cells grown in lipid-free medium. @entity3569 ovary cells maintained in culture medium supplemented with complete serum can grow at nearly normal rates in the presence of phospholipase C for many generations, even though the treatment enhances turnover of cellular @entity2434 (R. Sleight and C. Kent (1983) J. Biol. Chem. 258, 824-830). The phospholipase C treatment, however, was toxic to cells maintained in medium supplemented with delipidated serum. @entity6579 protected cells from phospholipase @entity137 , but did not support growth. The rate of utilization of @entity6579 for @entity2434 synthesis was about 8% of the turnover rate for @entity2434 , and was not increased by phospholipase C treatment. Reconstitution of the medium with fractionated serum lipids showed that the cells required specific neutral lipids, namely, @entity1718 plus @entity165 , in order to grow in the presence of phospholipase C. Either oleate or @entity165 (up to 80 microM) alone did not allow growth in phospholipase C, but a combination of these two lipids supported growth effectively.
