[Activation of transcription factors and induction of cytokines from macrophages in XXXX ].

@entity1 @entity3617 @entity650 @entity2451 @entity1356 @entity310 @entity11346 @entity1030 @entity687 @entity18720
OBJECTIVE: To investigate the activation of transcription factors and induction of cytokines from alveolar macrophages in @entity1030 ( @entity1030 ). METHODS: Alveolar macrophages were collected by fibrobronchoscopy from 8 @entity1 with chronic @entity3617 , 8 @entity1 with @entity1030 , and 8 healthy volunteers. All @entity1 were at stable stage. The macrophages thus collected were cultured and stimulated with lipopolysaccharide (LPS, 10 micrograms/ml). The @entity687 , @entity1356 , @entity310 and @entity650 thus produced were measured by ELISA in the supernatant. Nuclear factor-kappa B ( @entity2451 ), @entity11346 ( @entity11346 ), @entity18720 were detected by electrophoretic mobility shift assay. RESULTS: The concentration of @entity687 released from macrophages of @entity1 with @entity1030 at stable stage before LPS stimulation was about 3 times higher than that in the healthy control (F = 4.34, P < 0.05). The concentration of @entity687 released from macrophages in @entity1 with @entity1030 was increased further after LPS stimulation in comparison to that in healthy controls(F = 3.56, P < 0.05). The concentration of @entity1356 and that of @entity310 released from macrophages of @entity1030 @entity1 were further increased after LPS stimulation (P < 0.05) in the @entity1030 @entity1 , but there was no difference in the concentration of @entity1356 and between the control and @entity1030 @entity1 before LPS stimulation. The constitutive activity of @entity11346 and the activity of @entity2451 induced by LPS were higher in the @entity1 with @entity1030 than in the controls. CONCLUSION: The alverlar macrophages of @entity1 with @entity1030 at stable stage may release higher concentration of @entity687 and @entity1356 . LPS stimulation increases the release of @entity1356 and @entity310 of alveolar macrophages. Enhancement of activity of @entity2451 and @entity11346 may positively regulate the production of @entity687 and @entity1356 in the airflow obstruction.
