[Adenovirus-mediated @entity1655 gene transfer has neuroprotective effects in neonatal @entity35 following XXXX ].

@entity3406 @entity6098 @entity39 @entity75 @entity26 @entity1711 @entity1655 @entity1710 @entity14322 @entity35 @entity6667 @entity528
OBJECTIVE: To investigate the protective effects of adenovirus-mediated vascular endothelial growth factor (Ad- @entity6667 )165 gene transfer against @entity75 ( @entity75 ) in neonatal @entity35 . METHODS: Ad- @entity6667 recombinant adenovirus was constructed by bacterial homologous recombination technology. Seven-day-old @entity35 were randomly assigned to 4 groups: sham-operated (n=20), @entity75 (n=25), buffer-treated (n=20), and Ad- @entity6667 -treated (n=25). The @entity75 model was prepared by permanent occlusion of left common carotid artery, followed by exposure to 8% @entity26 for 2 hrs. In the Ad- @entity6667 -treated and the Buffer-treated groups, 2 microL recombinant adenovirus suspension or buffer was injected into the left sensorimotor cortex of the @entity35 brain 3 days after @entity75 . Seven days after transplantation, @entity1655 mRNA expression was detected using RT-PCR. Neuronal apoptosis was detected by the terminal deoxynucleotidyl transferase-mediated biotinylated @entity14322 triphosphate @entity3406 end labeling (TUNEL). @entity6098 and @entity6667 protein were detected using immunohistochemistry. Microvascular density in the cerebral cortex was measured based on @entity6098 positive cells. A radial arm maze test was performed from 30 postnatal days to evaluate long-term learning and memory functions. At 35 postnatal days, the @entity35 were sacrificed for cerebral histological examinations by @entity1710 and @entity1711 . RESULTS: The expression of @entity1655 mRNA increased in the Ad- @entity6667 -treated group more than in the untreated @entity75 and the buffer-treated groups (p<0.05). The number of apoptotic neurons was less in the Ad- @entity6667 -treated group compared with that in the untreated @entity75 and the buffer-treated groups (p<0.05). Microvascular density and @entity6667 positive cells increased in the Ad- @entity6667 -treated group compared with that in the untreated @entity75 and the buffer-treated groups (p<0.05). In the radial arm maze test, the Ad- @entity6667 -treated group had more improved achievements than the @entity75 and the buffer groups (p<0.05). @entity528 and @entity39 were lessened in the Ad- @entity6667 -treated group compared with the @entity75 and the buffer groups. CONCLUSIONS: Ad- @entity6667 gene transfer can increase the expression of @entity6667 mRNA and @entity6667 protein, decrease neuronal apoptosis, and increase angiopoiesis in the brain. This attenuates @entity75 and improves long-term learning and memory functions in neonatal @entity35 after @entity75 .
