@entity817 mutations, XXXX and @entity876 protein expression in sporadic basal cell carcinomas.

@entity124 @entity72401 @entity5 @entity1489 @entity72399 @entity535 @entity377 @entity22191 @entity876 @entity272 @entity817 @entity694 @entity72400 @entity320 @entity1491
@entity1491 ( @entity1491 ) is the most common @entity272 . Genes related to the Ras/Raf signalling pathway have been implicated in the pathogenesis of @entity1489 . The objective of this study was to investigate the presence of @entity817 mutations in sporadic BCCs as well as its correlation with the phenotype of @entity124 ( @entity124 ), the clinicopathological parameters of the @entity5 and @entity876 protein expression.  83 @entity1491 specimens were screened for @entity817 mutations, applying polymerase chain reaction, single-stranded conformation polymorphism (PCR-SSCP) and DNA sequencing. @entity124 status was examined using mononucleotide microsatellite markers and @entity876 protein expression was demonstrated by immunohistochemical staining. A @entity72399 @entity377 leading to a silent mutation @entity72400 ; and a T to A transversion causing an @entity535 change ( @entity72401 ) have been found. @entity124 was detected in 5% of the cases and @entity876 accumulation was present in 37/83 samples studied. Although rare @entity817 alterations have been observed in @entity1491 , none of them harboured the hot-spot mutation @entity22191 commonly present in @entity320 and @entity694 . Consequently, no correlation could be determined between @entity817 alterations, @entity124 status, the clinicopathological features and @entity876 protein expression. Our results are in favour of a secondary importance for Ras signalling cascade genes in @entity1491 pathogenesis.
