Expression of inducible XXXX synthase by stimulated macrophages correlates with their antihistoplasma activity.

@entity19 @entity534 @entity842 @entity13633 @entity3218
The antihistoplasma activity of recombinant @entity19 gamma interferon (rMuIFN-gamma)-treated macrophages of the RAW 264.7 cell line depends on the generation of @entity842 (NO.) from @entity534 . Macrophages of the @entity19 cell line treated with rMuIFN-gamma do not produce NO. or inhibit the intracellular growth of @entity3218 . NO. is generated by the inducible enzyme @entity842 synthase ( @entity13633 ) formed by stimulated macrophages. Northern (RNA) blot analysis of RAW 264.7 cells revealed the expression of @entity13633 mRNA after exposure to rMuIFN-gamma. In contrast, rMuIFN-gamma-treated @entity19 cells did not produce detectable levels of @entity13633 . These data suggest that the failure of @entity19 cells to generate NO. and to restrict the intracellular growth of @entity3218 is due to a lack of expression of @entity13633 following treatment with rMuIFN-gamma.
