Effects of selected xenobiotics on hepatic and plasmatic biomarkers in juveniles of XXXX .

@entity251 @entity5444 @entity3102 @entity16988 @entity2082 @entity7869 @entity858 @entity134 @entity1301 @entity8445
In recent years, @entity16988 has increasingly been used in pollution monitoring studies. In order to assess its response to some particular widespread pollutants, juveniles of @entity16988 were administered an intraperitoneal injection of the model aryl hydrocarbon receptor agonist b-naphtoflavone (bNF) and chemicals of environmental concern, such as the fungicide @entity3102 (KETO), the lipid regulator @entity8445 (GEM), the surfactant @entity1301 (NP) and the synthetic hormone @entity2082 ( @entity7869 ). Two days after injection, the effect of these chemicals was followed up as alterations of hepatic microsomal activities of the cytochrome P450 (CYPs) and associated reductases, carboxylesterases (CbEs) and the conjugation enzyme @entity858 glucuronyltransferase (UDPGT). In the cytosolic fraction of the liver, the effect on CbEs, glutathione S-transferase (GST) and antioxidant activities was also considered. Alterations on the endocrine reproductive system were evaluated by plasma levels of vitellogenin (VTG) and the sex steroids estradiol (E2), @entity251 (T), @entity5444 (11KT) and the progestin 17a,20b-dihydroxy-4-pregnen-3-one (17,20b-P). Injection with the model compound bNF induced the hydrolysis rate of the seven CYP substrates assayed. The xenobiotic GEM induced three CYP-related activities (e.g. ECOD) and UDPGT, but depressed antioxidant defenses. @entity7869 induced four CYPs, more significantly ECOD and BFCOD activities. The xenoestrogens NP and @entity7869 altered the activities of CbE in microsomes and catalase, and were the only treatments that induced de novo VTG synthesis. In addition, the progestin 17,20b-P, was induced in NP-injected fish. None of the treatments caused statistically significant effects on @entity134 plasma levels. In conclusion, the CYP substrates assayed responded specifically to treatments and juveniles of @entity16988 appear good candidates for assessing xenobiotics exposure.
