Retinal antigen specific lymphocytes, TCR-gamma delta T cells and XXXX + B cells cultured from the vitreous in @entity697 .

@entity3647 @entity697 @entity404 @entity1075 @entity75 @entity5893 @entity3138 @entity405 @entity205 @entity2071 @entity2902 @entity1199 @entity792
@entity5893 + B lymphocytes and TCR gamma-delta T lymphocytes, phenotypes implicated in the pathogenesis of @entity792 , were isolated from the vitreous in a case of @entity697 . These cells were obtained using a method which allows the selective maintainance in vitro of in vivo activated T lymphocytes. Dual colour flow cytometry showed that after 3 days culture in @entity1199 containing medium 61% of cells were @entity5893 / @entity1075 + ve and 41% CD3/TCR gamma delta + ve. Of the total CD3 + ve population, 15% were gamma/delta negative. These cells formed a population which also responded in a proliferation assay to retinal antigens. Histologically the eye showed a marked mononuclear cell infiltration of the retina, ciliary body and choroid. @entity75 within the choroid contained lymphocytes, plasma cells and multinucleate giant cells. Immunocytochemistry showed lymphocyte populations to be predominantly @entity3138 + ve CD3 + ve T lymphocytes of the @entity404 sub-set. Distribution of monocytes/macrophages throughout the lesions and restriction of B-lymphocytes to granulomata were all consistent with a DTH type reaction. Despite immunosuppressive therapy, the expression of activation antigens HLA-DR and @entity2071 on infiltrating and resident ocular tissue cells was high, although @entity3647 ( @entity2902 ) expression was virtually absent. Flow cytometric analysis of peripheral blood cells prior to treatment with @entity205 showed systemic activation of lymphocytes, with high levels of HLA-DR and @entity2902 expression and a raised @entity404 / @entity405 ratio.
