@entity4571 inhibits tissue factor expression in isolated XXXX pancreatic islets: implications for clinical islet transplantation.

@entity1 @entity4688 @entity534 @entity2842 @entity205 @entity4571
BACKGROUND: Islet-produced tissue factor (TF) triggers an adverse clotting reaction, the instant blood-mediated inflammatory reaction (IBMIR), providing a likely explanation for the need of tissue from multiple donors in clinical islet transplantation. In this study, the authors investigated whether compounds previously shown to affect TF and @entity2842 expression in monocytes and endothelial cells have the same effect in @entity1 islet cells. METHODS: Islets were cultured in the presence of @entity534 , @entity205 , @entity4688 , or @entity4571 for 48 hr, after which the TF content and @entity2842 expression were assessed. The effect of @entity4571 on IBMIR was evaluated by exposing the treated islets to fresh @entity1 ABO-compatible blood in an in vitro loop model. RESULTS: @entity4571 was the only compound that significantly reduced both TF and @entity2842 . This reduction was dose-dependent. The level of @entity2842 was strongly correlated with TF expression (r2=0.98). In addition, the level of TF was also correlated with the ability of the islets to initiate IBMIR (r2=0.94). CONCLUSIONS: TF and @entity2842 expression in @entity1 islets can be decreased by adding @entity4571 to the culture medium. These observations indicate that the adverse effects of IBMIR in clinical islet transplantation could be reduced without endangering the recipient using antithrombotic drugs.
