Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of @entity16509 and XXXX -y in 3T3-L1 adipocytes.

@entity3229 @entity3208 @entity28 @entity16509 @entity412 @entity2975 @entity214 @entity21170 @entity15 @entity413 @entity165
UNASSIGNED: Recently, @entity28 has increased due to a variety of reasons, including the availability of 'fast food' and high-fat diets. Developing anti- @entity28 functional drugs and foods from natural sources may offer solutions to this global concern. Generally, tuna is a high-protein, low-fat and low-calorie food with various bioactive effects. It may improve memory, reduce @entity165 levels and positively affect the development of brain cells. In this study, we screened the anti- @entity28 potential of peptides derived from tuna protein. We then observed protein bands by the Coomassie blue staining of a @entity3208 - @entity2975 gel electrophoresis ( @entity214 -PAGE) gel. The protein mixture was concentrated and desalted using in-gel trypsin digestion and a C18  nano column and Poros  R2 reversed-phase preparation, prior to quadrupole time-of-flight mass spectrometry (Q-TOF @entity15 / @entity15 ). We screened the peptides for their ability to affect adipogenesis in 3T3-L1 adipocytes. We also measured @entity413 uptake, @entity412 levels and lipid droplets using @entity21170 staining. As a result, we confirmed that one peptide inhibited adipocyte differentiation. We also observed the expression of @entity28 -related genes by western blot analysis and reverse transcription-polymerase chain reaction. The peptide from the tuna extract significantly reduced the expression levels of CCAAT/enhancer-binding protein  a ( @entity16509 ) and peroxisome proliferator-activated receptor-y ( @entity3229 -y) adipocyte marker genes. Thus, our data suggest that this peptide from boiled tuna extract reduces lipid components and adipogenesis in 3T3-L1 cells, and these characteristics may be of value in the development of anti- @entity28 foods.
