[Study on so called "cellular effect" of methotrexate on XXXX and its mode of manifestation (author's transl)].

@entity1420 @entity1466 @entity6740 @entity53 @entity1500 @entity1815 @entity230
The "cellular effect" i.e. temporary elevation of @entity6740 titer may not be infrequently experienced during course of chemotherapy against @entity1466 . Present paper is to clarify real view of the effect and mechanism of its manifestation by using a cell-line (GCH-nu). 1) Cellular growth was suppressed moderately by 10(-7), 10(-6) and 10(-5)M @entity1500 . 2) During 2-4 days of administration at these concentrations of @entity1500 @entity6740 per ml medium was elevated 5 times. @entity6740 in cells per mg protein were also higher 5-7 times. 3) @entity6740 -beta in media was enhanced 5-7 times in @entity1500 group. 4) @entity1815 , @entity53 and @entity1420 were not altered. 5) @entity1466 cells developed marked enlargement of nuclei and cells, multinucleation and cytoplasmic vacuolization. 6) Cytochemistry of heat stable ALP revealed increased activity especially in cells with morphological changes. ALP examined by biochemical method was enhanced 5 times. 7) Amount of protein per cell increased 10 times after giving @entity1500 . Real view of so-called "cellular effect" includes elevations of @entity6740 -beta and ALP activity as well as @entity6740 , whereas sex @entity230 are not apparently related. The data in addition to morphological and cytochemical changes reveal that @entity1500 may induce differentiation of @entity1466 cells into those of syncytial type, which then sustain the "cellular effect".
