Expression of cellular oncogenes in primary cells from XXXX @entity3052 .

@entity1 @entity1861 @entity981 @entity7242 @entity13327 @entity984 @entity5 @entity517 @entity3052 @entity1778 @entity1639 @entity8497 @entity11511
The structure and the expression of 11 cellular oncogenes (protooncogenes) were analyzed in primary cells from 20 acute lymphocytic ( @entity981 ) and 31 @entity3052 ( @entity3052 ) @entity1778 @entity1 . Neoplastic cells, obtained prior to initiation of therapy, were purified and classified, on the basis of both surface antigen pattern and morphology, into pre-B, B, and @entity3052 . RNA was extracted and analyzed for expression of cellular oncogenes coding for nuclear proteins ( @entity517 , @entity13327 , @entity1639 ), the beta-chain of platelet-derived growth factor (c-sis), growth factor receptors or related proteins ( @entity7242 , @entity1861 , c-fes, c-erbB), or putative intermediate transducers of mitogenic signals (c-Ha-ras, @entity984 , c- @entity8497 ). Quantitative analysis of total RNA was carried out by dot blot hybridization to specific cDNA or genomic probes. Number and size of transcripts were evaluated by blot hybridization of electrophoretically fractionated poly(A)+ RNA. Expression of @entity517 and @entity13327 was detected in all @entity1778 cells at variable levels and was characterized by well-defined patterns within @entity981 subtypes. Conversely, significant levels of @entity1639 transcripts were detected only in myelomonocytic (M4) and @entity1778 . Among the "src-family," c-fes was expressed more in @entity3052 than @entity981 , and @entity1861 was expressed at variable but not elevated levels in all @entity1778 types. c-Ha-ras was uniformly expressed at low levels, as in non-neoplastic cells. @entity984 transcription was detected only in @entity11511 ; @entity8497 expression was barely demonstrable. The structure of these protooncogenes was not grossly modified, as evaluated by Southern analysis, except for @entity517 rearrangement in B @entity981 . These studies indicate that cellular oncogene expression in specific subtypes of @entity1778 cells may relate to either the proliferative activity ( @entity517 , @entity13327 ) or the differentiation state ( @entity1639 ) of the cells, or possibly to expression of receptors for putative hemopoiesis-related growth factors (c-fes, @entity1861 ). Our data provide a basis for in-depth analysis of protooncogene expression in normal and @entity5 .
