@entity3546 and @entity8889 are prognostic markers in XXXX .

@entity1 @entity5 @entity470 @entity149 @entity11 @entity3546 @entity8889 @entity876 @entity463
@entity463 ( @entity463 ), the most common primary @entity149 , are highly aggressive and heterogeneous, and remain a dramatic therapeutic challenge. Markers mirroring the complex molecular profile of @entity463 that are predictive of @entity1 outcomes are needed to define novel multi-targeted treatment strategies. Resistance to current @entity11 therapies is partly due to a subpopulation of stem-like and other self-renewing cells (hereafter called glioma stem-like @entity5 cells, GSCC), which are therefore of key interest as therapeutic entry points. Wnt and Hedgehog are among the main pathways involved in GSCC renewal. @entity3546 and @entity8889 are markers of Wnt and Hedgehog activation respectively and both pathways are known to be altered in @entity11 . To date, there are no investigations of @entity8889 protein expression in @entity11 tissue, and recently a high expression of @entity3546 has been found to have a poor prognostic impact in @entity11 @entity1 in a study. We have therefore quantified the positivity for @entity3546 , @entity8889 , as well as Ki-67, @entity876 , and @entity470 proteins on immunohistochemically-stained @entity11 sections from 106 @entity1 in an investigation for potential predictive biomarkers. Correlation between these markers and survival was evaluated by pair-wise Pearson correlation coefficient and by bi-dimensional hierarchical clustering, followed by survival estimations using linear regression models and classification trees. We demonstrated that both @entity3546 and, for the first time, @entity8889 proteins are highly predictive markers of short survival, being found in 75 and 90% of the highly predictive trees, respectively, whereas Ki-67, @entity876 and @entity470 were under 30% and thus, not considered as predictive. Our results indicate a role of @entity3546 and @entity8889 in @entity11 malignant behaviour, and suggest that inhibiting members of Wnt and Hedgehog pathways could be a valuable therapeutic strategy for @entity11 @entity1 .
