Light scatter and cell surface properties of XXXX megakaryocyte progenitor cells.

@entity3167 @entity19 @entity3844 @entity2822 @entity2823 @entity3505 @entity6409
The characteristics with respect to light scatter and cell surface properties of megakaryocyte colony-forming cells (CFU-Meg), growing in serum-free agar cultures, have been determined. @entity19 bone marrow cells were fractionated using a light-activated cell sorter (FACS) either on the basis of light scatter or after staining with a @entity2822 ( @entity2823 )-labeled lectin or monoclonal antibody. After staining and sorting, the recovery of CFU-Meg was about 50%. In the unsorted controls 29 +/- 2 CFU-Meg/10(5) cells were observed. The forward (FLS) and perpendicular ( @entity6409 ) light scatter intensities of CFU-Meg were very similar to those of spleen colony-forming cells (CFU-S) and early progenitor cells. The average diameter of the CFU-Meg as determined by the FLS intensity profile was about 7.5 microns. @entity3505 germ agglutinin binding of CFU-Meg was high, indicating a high @entity3167 content of the cell surface. Expression of the differentiation antigen @entity3844 on the CFU-Meg as determined by indirect immunofluorescence with the rat monoclonal antibody I42/5.1 was moderate. @entity3844 expression was lower than the @entity3844 expression of granulocyte/macrophage progenitors (CFU-C) or granulocytes. It is concluded that the light scatter and cell surface properties of CFU-Meg are similar to those of CFU-S and early committed progenitors.
