Isolation and preliminary characterization of proteoglycans dissociatively extracted from XXXX aorta.

@entity1 @entity39697 @entity17540 @entity2097 @entity6928 @entity7597 @entity597
Aortic proteoglycans (PG) were isolated from @entity1 aorta intima-media preparations with 4 M @entity2097 in the presence of protease inhibitors. The extracted PG mixture comprised 67% of the total aortic PG and was composed of 65% chondroitin @entity597 , 22% dermatan @entity597 , 8% heparan @entity597 , and 4% @entity17540 . Attempts at isolation and purification of PG monomers using isopycnic @entity6928 gradient centrifugation under associative and dissociative conditions resulted in appreciable loss of PG through associations with co-extracted aortic proteins. The addition of a gel chromatographic step on Sepharose CL-4B under dissociative conditions resulted in separation of PG from the majority of co-extracted proteins. In addition, the procedure resulted in a separation of the PG into a population ( @entity7597 ) eluting near the column V0 and one ( @entity39697 ) included with a Kav of 0.38. Hyaluronic acid co-eluted with @entity7597 . The major glycosaminoglycan in @entity7597 was chondroitin @entity597 , (85 to 95%). No dermatan @entity597 was detected in @entity7597 , but this glycosaminoglycan was the predominant glycosaminoglycan in @entity39697 (50 to 70%). Heparan @entity597 was present in small amounts in both @entity7597 and @entity39697 . Data presented support the proposal of at least three species of PG monomers in the aortic wall. Chromatographic studies under dissociative and associative conditions indicated that PG comprising @entity7597 but not @entity39697 were capable of associations with hyaluronic acid.
