Prognostic value of DNA content in XXXX . A flow cytometric study with some methodologic aspects.

@entity1 @entity2007 @entity5 @entity74 @entity14
The DNA content of 37 @entity14 was studied prospectively by flow cytometry. The DNA content from the same @entity5 was also studied in disintegrated paraffin sections. The methods gave similar information on the DNA content and the correlation was 0.94. In eight @entity1 @entity5 imprints were prepared and the DNA content was analyzed with static cytometry. A correlation coefficient of 0.83 between flow cytometry on fresh @entity5 tissue and static cytometry was found. Sixty-two percent of the @entity5 were @entity2007 and had significantly higher S-phase values than @entity5 . Histologic grade was not related to ploidy level, whereas @entity5 often were @entity2007 . When the clinical course of @entity1 was analyzed by log rank test (mean follow-up, 30.4 months), the @entity1 with @entity5 showed a clear advantage in terms of survival; only two @entity1 in this group developed @entity74 . It is concluded that the DNA content is an independent prognostic predictor of @entity14 and that DNA can be analyzed by several methods. Of these, the method using disintegrated paraffin sections appears most attractive.
