XXXX dependency of massive postmortem bronchoconstriction in @entity840 lungs.

@entity840 @entity37239 @entity2507 @entity460 @entity594 @entity5143
@entity460 antagonists were employed to examine whether or not @entity840 's postmortem bronchoconstriction is @entity460 dependent. First, a group of 17 young (3-4-week-old) animals were divided in three subgroups: control, @entity594 , and @entity37239 ( @entity37239 , an intracellular @entity460 antagonist). A second group of 12 mature (5-7-week-old) animals were divided into two subgroups: control and @entity594 . Baseline pressure-volume (P-V) curves were performed prior to cannulation of the pulmonary artery in the anesthetized, opened chested animal. The lungs of the animal were then perfused and P-V curves were obtained again at intervals for up to 60 min. @entity594 (10(-6) M) or TMB-8 (5 X 10(-4) M) was added to the perfusate in experimental groups. A temporal decrease in inflation volume (IV, the lung volume between transpulmonary pressure of 0 and 30 cm @entity2507 during inflation) was used as an indicator of bronchoconstriction. In control subgroups, the decrease in IV was significantly greater (P less than 0.05) in the young (29.5 +/- 12.9% baseline at 20 min) than in the mature animals (75.0 +/- 7.0% baseline at 20 min). @entity594 significantly alleviated (P less than 0.05) the decrease in IV in young animals at 15-30 min (58.6 +/- 8.4% baseline at 20 min) while TMB-8 (90.6 +/- 2.1% baseline at 20 min) prevented the decrease even further. @entity594 did not have a significant effect on IV in mature animals. These data suggest that the postmortem @entity5143 is much more severe in young animals and that this severe constriction is @entity460 dependent.
