Hyaluronic acid content of effusions as a diagnostic aid for XXXX .

@entity1143 @entity1 @entity7245 @entity5 @entity3620 @entity1391
A high-performance liquid chromatographic technique, using a size exclusion column (TSK-5000PW), has been developed for the quantification of @entity7245 ( @entity7245 ) in @entity1143 . Sample preparation requires only a 100-fold dilution of the exudate with @entity1391 buffer prior to analysis. Chromatographic conditions are: 0.05 M @entity1391 buffer (pH, 5.0) mobile phase at a flow rate of 1.0 ml/min, ultraviolet absorbance detection at 200 nm. The method resolves @entity7245 from all other glycosaminoglycans. The presence of @entity7245 is confirmed by the removal of the @entity7245 peak (retention time, approx. 5.3 min) by incubation of a second sample aliquot with hyaluronidase. Effusions of 13 of 14 @entity1 with confirmed @entity3620 contained @entity7245 in the 0.3 to 11.1 mg/ml range. In only one case was no @entity7245 detected. None of the effusions from 56 control @entity1 with various other @entity5 contained detectable @entity7245 , i.e., there were no false positives. An unidentified peak, not susceptible to hyaluronidase appeared in 11% (6 of 56) of the controls. A single mesothelioma case was correctly identified in a group of 10 coded samples. It is suggested that an effusion with an @entity7245 concentration greater than 0.25 mg/ml, confirmed by hyaluronidase susceptibility, is an indication of the presence of @entity3620 . The test is simple and rapid, and it is recommended that any effusion of uncertain etiology be screened for the presence of @entity7245 .
