@entity4047 Augments Compressive Stress-Induced Tissue Factor Expression in XXXX Airway Epithelial Cells.

@entity1 @entity565 @entity19 @entity4047
Tissue factor (TF) is best known as a cellular initiator of coagulation, but it is also a multifunctional protein that has been implicated in multiple pathophysiologic conditions, including @entity565 . In the lung, airway epithelial cells express TF, but it is unknown how TF expression is regulated by @entity565 -associated mediators. We investigated the role of @entity4047 , a type 2 cytokine, alone and in combination with compressive stress, which mimics asthmatic bronchoconstriction, on TF expression and release of TF-positive extracellular vesicles from primary normal @entity1 bronchial epithelial cells. Well-differentiated normal @entity1 bronchial epithelial cells were treated with @entity4047 and compressive stress, alone and in combination. TF mRNA, protein and activity were measured in the cells and conditioned media. TF was also measured in the bronchoalveolar lavage (BAL) fluid of allergen-challenged @entity19 and @entity1 with @entity565 . @entity4047 and compressive stress increased TF expression, but only compressive stress induced TF-positive extracellular vesicle release. Pretreatment with @entity4047 augmented compressive stress-induced TF expression and release. TF protein and activity in BAL fluid were increased in allergen-sensitized and -challenged @entity19 . TF was elevated in the BAL fluid of @entity1 with mild @entity565 after an allergen challenge. Our in vitro and in vivo data indicate close cooperation between mechanical and inflammatory stimuli on TF expression and release of TF-positive extracellular vesicles in the lungs, which may contribute to pathophysiology of @entity565 .
