Monoclonal antibodies with various reactivity to XXXX killer inhibitory receptors.

@entity1 @entity9371 @entity16301 @entity2940 @entity2936 @entity28383 @entity20212 @entity32883
@entity1 natural killer (NK) cells have receptors that bind to HLA class I molecules. Among these receptors, @entity28383 and @entity2936 bind to HLA-C. @entity28383 belongs to the @entity9371 ( @entity9371 ) and @entity2936 belongs to the killer activatory receptor (KAR). Previously, we obtained three recombinant @entity28383 @entity9371 or @entity2936 KAR proteins, KAR-K1 ( @entity32883 ), @entity9371 -K6 ( @entity16301 ), and @entity9371 -K7 ( @entity28383 ). In this study, we produced and characterized seven monoclonal antibodies (MAbs) to the @entity28383 @entity9371 and @entity2936 KAR proteins. The MAbs were classified in three groups according to their antigen-binding specificity: 5IB103 and 26ID707 were KAR-K1-specific; A809, 190IIC311, and 197IIC611 were @entity9371 -K7-specific; while A210 and A803g bound to all three recombinant proteins. The MAbs reactive to @entity9371 -K7 bound to the gamma3 domain among two immunoglobulin (Ig) domains of @entity9371 -K7. Immunofluorescence staining and flow cytometric analysis with A803g showed reactivity to about 10% of peripheral blood mononuclear cells and 35% of purified natural killer cells. Double immunofluorescence staining with @entity20212 and anti- @entity2940 Ab showed that @entity2940 and @entity28383 or @entity2936 were expressed on NK cells in a mutually exclusive way. We also investigated T-cell markers in A803g+ cells. A803g+ T cells were almost CD8+ cells. MAbs produced in this study can be utilized practically in the investigation of biological characteristics of @entity28383 @entity9371 and @entity2936 KAR.
