Cryoprotective Effect and Optimal Concentration of XXXX on Aortic Valve Homografts.

@entity7970 @entity1568 @entity95 @entity3733 @entity730 @entity413
BACKGROUND AND AIM OF THE STUDY: Cryopreservation allows for the long-term storage of aortic homografts, but a high risk of @entity730 and degeneration is often observed following their transplantation. The study aim was to investigate the cryoprotective effect of @entity7970 on aortic valve homografts preserved in liquid @entity1568 , and to determine the optimal @entity7970 concentration for such purpose. METHODS: Aortic valve homografts obtained from New Zealand White @entity95 were processed using different protectants. Samples were assigned at random to four groups: a control group treated with @entity3733 ( @entity3733 ; 0.1 mol/l), and test groups A, B and C, which were treated with 0.1 mol/l @entity7970 + 0.1 mol/l @entity3733 , 0.2 mol/l @entity7970 + 0.1 mol/l @entity3733 , or 0.3 mol/l @entity7970 + 0.1 mol/l @entity3733 , respectively, as protectant. Samples in each group were allocated randomly to three subgroups and cryopreserved for 12, 15, and 18 months, respectively. After thawing, apoptosis of the cryopreserved homograft samples was evaluated using immunohistochemistry, semi-quantitative RT-PCR and Western blotting methods. The viability of the tissue cells was assessed by monitoring @entity413 utilization capacity. RESULTS: The apoptosis assay showed that, among the four groups and at all time points, the expression of caspase-3 was lowest in test groups A and B and highest in the @entity3733 group. In comparison, the @entity413 metabolic rates of test groups A and B were highest, while rates for test group C and the control ( @entity3733 ) group ranked second and third. CONCLUSION: When aortic homografts are preserved in liquid @entity1568 , the cryoprotective effect of @entity7970 combined with @entity3733 was superior to that of @entity3733 alone. The optimal @entity7970 concentration to cryoprotect @entity95 aortic homografts was between 0.1 and 0.2 mol/l.
