Specific detection of XXXX in the effluent of a gas-chromatographic column by on-line measurement of fluorescence.

@entity1522 @entity1472 @entity3884 @entity2229 @entity3265 @entity20720 @entity6115 @entity4478
Compounds with primary @entity1472 groups react with @entity20720 in solution to yield highly fluorescent products. This reaction is now in wide use for detecting @entity1522 and @entity4478 in liquid-chromatography effluents. We report here a method for using @entity20720 to detect @entity2229 in the effluent of a gas-liquid-chromatographic column. The effluent gas, nominally 20 mL/min, is delivered to a scrubber consisting of a small-bore, simulated capillary chromatographic column that is simultaneously supplied with 1 mL/min of the reagent solution. The liquid effluent of the scrubber, separated from the gas, is drawn through the flow cell of a fluorometer. Short-chain @entity4478 and @entity3265 were quantitatively scrubbed. The response of the fluorometer was directly proportional to the number of nanomoles injected into the column. Less than a nanomole of @entity6115 was detectable. Comparison of results with those from a @entity3884 flame-ionization detector showed minimal additional peak broadening or compromise of resolution. These results demonstrate the feasibility of using highly specific, as well as sensitive, liquid-chromatographic detection methods for gas-liquid chromatography.
