Periodontal disease level- XXXX amounts locally administered in the @entity35 @entity7138 induce ER stress in the systemic blood.

@entity1 @entity32 @entity21206 @entity2520 @entity141 @entity14559 @entity262 @entity4877 @entity22780 @entity35 @entity4026 @entity7138
UNASSIGNED: Periodontal diseases have long been postulated to contribute to @entity262 and, likewise, it has been proposed that @entity2520 treatment may ameliorate certain @entity262 . @entity22780 ( @entity14559 ) are major secondary metabolites produced by oral anaerobic bacteria and, among the SCFAs, @entity4877 (BA) in high amounts contribute to @entity2520 development. Periodontal disease level- @entity4877 (PDL-BA) is found among @entity1 suffering from @entity2520 and has previously shown to induce oxidative stress, whereas, oxidative stress is correlated to endoplasmic reticulum (ER) stress. This would imply that PDL-BA may likewise stimulate ER stress, however, this was never elucidated. A better understanding of the correlation between PDL-BA and systemic ER stress stimulation could shed light on the possible systemic effects of PDL-BA-related @entity2520 . Here, PDL-BA was injected into the @entity7138 and the systemic blood obtained from the @entity35 jugular was collected at 0, 15, 60, and 180  min post-injection. Collected blood samples were purified and only the blood cytosol was used throughout this study. Subsequently, we measured blood cytosolic @entity21206 , @entity141 , representative apoptotic and inflammatory caspases, and @entity4026 amounts. We found that PDL-BA presence increased blood cytosolic @entity21206 and @entity141 amounts. Moreover, we observed that blood cytosolic caspases and @entity4026 were activated only at 60 and 180  min post-injection in the @entity35 @entity7138 . This suggests that PDL-BA administered through the @entity7138 may influence the systemic blood via ER stress stimulation and, moreover, prolonged PDL-BA retention in the @entity7138 may play a significant role in ER stress-related caspase and @entity4026 activation. In a @entity2520 scenario, we propose that PDL-BA-related ER stress stimulation leading to the simultaneous activation of apoptosis and @entity32 may contribute to @entity2520 pathogenesis.
