Trafficking of Estrella lausannensis in XXXX Macrophages.

@entity1 @entity3220 @entity55 @entity21216 @entity15668 @entity1047
UNASSIGNED: Estrella lausannensis is a new member of the Chlamydiales order. Like other @entity1047 it is able to replicate in amoebae and in fish cell lines. A preliminary study investigating the pathogenic potential of @entity1047 found a correlation between antibody response to E. lausannensis and @entity55 in @entity1 . To further investigate the pathogenic potential of E. lausannensis, we determined its ability to grow in @entity1 macrophages and its intracellular trafficking. The replication in macrophages resulted in viable E. lausannensis; however, it caused a significant cytopathic effect. The intracellular trafficking of E. lausannensis was analyzed by determining the interaction of the Estrella-containing inclusions with various endocytic markers as well as host organelles. The E. lausannensis inclusion escaped the endocytic pathway rapidly avoiding maturation into phagolysosomes by preventing both @entity21216 and @entity3220 accumulation. Compared to @entity15668 , another @entity1047 , the recruitment of mitochondria and endoplasmic reticulum was minimal for E. lausannensis inclusions. E. lausannensis appears to use a distinct source of nutrients and energy compared to other members of the Chlamydiales order. In conclusion, we hypothesize that E. lausannensis has a restricted growth in @entity1 macrophages, due to its reduced capacity to control programmed cell death.
