Stability and activity of specific antibodies against @entity6586 and @entity6586 sobrinus in XXXX milk fermented with @entity1905 or treated at ultra-high temperature.

@entity1 @entity6586 @entity692 @entity1547 @entity1200 @entity1905 @entity291
Passive local immunization against @entity1547 is a promising approach to its prevention, as clinical evidence of active oral or nasal immunization is still limited and controversial. By means of systemic immunization of pregnant @entity291 with a multivalent vaccine, high titres of IgG antibodies against @entity1 cariogenic bacteria, @entity6586 and @entity6586 sobrinus, were produced in @entity291 colostrum. The purified @entity1200 ( @entity1200 ) of this preparation has a number of anticariogenic properties, such as inhibition of streptococcal adherence to saliva-coated @entity692 and inhibition of glucosyltransferase enzymes. This study investigated whether @entity1200 antibodies remained active and functional when added to ultra-high temperature (UHT)-treated milk or to @entity1905 (LGG)-fermented milk stored for an extended time. LGG was chosen because of its widely known health benefits in @entity1 and animals. A commercial UHT toddler's milk was supplemented with @entity1200 and stored for 2 months at 5, 21 and 30 degrees C. The antistreptococcal titres in UHT milk did not decline at any temperature during storage, and UHT- @entity1200 inhibited the adherence of @entity6586 for up to 2 months. This was not the case with UHT toddler's milk without IgG antibodies. Milk was fermented with live LGG cells in the presence or absence of 5% @entity1200 . The antistreptococcal titres declined to about 30% of the original titres after storage. Fresh milk alone slightly enhanced streptococcal adhesion but fresh milk with @entity1200 inhibited the adherence of @entity6586 by over 50%. LGG-positive fermented milk without antibodies also inhibited (P < 0.05) the adhesion by about 40%. In both LGG-fermented and UHT immune milk, the activity of antibodies against cariogenic streptococci was maintained during the expected shelf-life of these products. From the anticariogenic point of view it may be beneficial to add bovine-specific antibodies against mutans streptococci to probiotic LGG-containing milk products.
