NMR structural study of fructans produced by XXXX B6, bacterium isolated in cloud water.

@entity44627 @entity8220 @entity42694 @entity7787 @entity948 @entity72708 @entity44628 @entity12315 @entity7016 @entity969 @entity19766 @entity72707 @entity413
@entity8220 B6, bacterium isolated from cloud water, was incubated on @entity969 for @entity19766 production. Dialysis of the obtained mixture (MWCO 500) afforded dialyzate ( @entity12315 ) and retentate (RIM). Both were separated by size exclusion chromatography. RIM afforded eight fractions: levan exopolysaccharide (EPS), fructooligosaccharides (FOSs) of levan and inulin types with different degrees of polymerization (dp 2-7) and monosaccharides @entity948 : @entity413 =9:1. Levan was composed of two components with molecular mass ~3500 and ~100kDa in the ratio 2.3:1. @entity7016 fraction contained difructose anhydride DFA IV. @entity44627 , @entity72707 , and @entity72708 were identified as @entity42694 in the ratio 2:1:3. Fractions with dp 4-7 were mixtures of FOSs of levan (2,6-bFruf) and inulin (1,2-bFruf) type. DIM separation afforded two dominant fractions: @entity7787 with @entity948 : @entity413 ratio 1:3; @entity7016 fraction contained @entity969 only. DIM trisaccharide fraction contained @entity44627 , @entity72707 , and @entity72708 in the ratio1.5:1:2, penta and hexasaccharide fractions contained FOSs of levan type (2,6-bFruf) containing a-glucose. In the pentasaccharide fraction also the presence of a homopentasaccharide composed of 2,6-linked bFruf units only was identified. @entity44628 , inulin (1,2-bFruf) type, was identified as DIM tetrasaccharide. Identification of levan 2,6-bFruf and inulin 1,2-bFruf type oligosaccharides in the incubation medium suggests both levansucrase and inulosucrase enzymes activity in @entity8220 B6.
