Effects of Incorporating @entity5070 Chitosan into XXXX Bone Cement Containing @entity1499 .

@entity4625 @entity1 @entity6822 @entity5 @entity840 @entity2 @entity5070 @entity1499 @entity4653
UNASSIGNED: Treatment of bone metastases usually includes surgical resection with local filling of @entity1499 ( @entity6822 ) in @entity4625 ( @entity4625 ) cement. We investigated whether incorporating @entity5070 chitosan (CMCS) in @entity6822 - @entity4625 cement might overcome disadvantages associated with @entity6822 . To determine the optimal CMCS+ @entity6822 concentration to suppress the viability of @entity5 cells, an integrated microfluidic chip culturing highly metastatic @entity2 cells (H460) was employed. The mechanical properties, microstructure, and @entity6822 release of (CMCS+ @entity6822 )- @entity4625 cement were evaluated respectively by universal mechanical testing machine, @entity4653 ( @entity4653 ), and incubation in simulated body fluid with subsequent HPLC-MS. Implants of @entity6822 - @entity4625 and (CMCS+ @entity6822 )- @entity4625 cement were evaluated in vivo in @entity840 femurs over time using spiral computed tomography with three-dimensional image reconstruction, and @entity4653 at 6 months. Viability of @entity1 cells was significantly lowest after treatment with 57  g/mL CMCS + 21  g/mL @entity6822 , which was thus used in subsequent experiments. Incorporation of 1.6% (w/w) CMCS to @entity6822 - @entity4625 significantly increased the bending modulus, bending strength, and compressive strength by 5, 2.8, and 5.2%, respectively, confirmed by improved microstructural homogeneity. Incorporation of CMCS delayed the time-to-plateau of @entity6822 release by 2 days, but increased the fraction released at the plateau from 3.24% ( @entity6822 - @entity4625 ) to 5.34%. Relative to the controls, the (CMCS+ @entity6822 )- @entity4625 implants integrated better with the host bone. @entity4653 revealed pores in the cement of the (CMCS+ @entity6822 )- @entity4625 implants that were not obvious in the controls. In conclusion, incorporation of CMCS in @entity6822 - @entity4625 appears a feasible and effective modification for improving the anti- @entity5 properties of @entity6822 - @entity4625 cement.
