Multiple chromosomal aberrations and 11p allelotyping in XXXX cell lines.

@entity1 @entity5 @entity2 @entity72882 @entity8587 @entity461 @entity39649
Cytogenetic and molecular genetic studies have implicated many chromosomal aberrations in the pathogenesis of @entity2 . Deletions on 3p and 9p are presently the primary target for positional cloning of putative @entity5 suppressor genes. We have recently reported frequent loss of heterozygosity in three separate regions (HRAS, D11S12, D11S16) on 11p in freshly resected @entity2 specimens. Here we report cytogenetic and molecular genetic analyses of 26 permanently growing @entity1 @entity2 cell lines. Deletions indicating regions which may harbor potential @entity5 suppressor genes were found in 5/9 cell lines on 2p, 5/9 on 2q, 6/9 on 3p, 7/9 on 3q, 5/9 on 6q, 3/9 on 9p, 5/9 on 11p, and 6/9 on 13q. Reduction to hemizygosity or a statistically significant increase in the frequency of homozygosity on 11p was found for all markers investigated except for @entity39649 ( @entity72882 ). Eight of twenty-six (31%) cell lines were hemizygous for D11S12 and 9/26 (35%) for D11S16. Seventeen of eighteen (94%) cell lines were homozygous for @entity461 (expected homozygosity, 53%), 15/15 (100%) for @entity8587 (expected homozygosity, 55%), and 16/18 (89%) for CAT (expected homozygosity, 50%). These results confirm the notion that 11p harbors several putative @entity5 suppressor genes which may become inactivated at different stages of @entity5 development and progression. They also provide a basis for selecting cell lines for genetic complementation specifically targeted at the regions described.
