Partial XXXX sequence of a cellulase-like component with IgE-binding properties from Stachybotrys chartarum.

@entity535 @entity1
BACKGROUND: The aim of this study was to characterize the @entity535 sequence of a selected Stachybotrys chartarum component and to investigate @entity1 IgE reactivity against components of S. chartarum and nine other fungal species. METHODS: @entity1 IgE reactivity against S. chartarum and nine other fungal extracts was investigated by the immunoblotting method. For automated @entity535 sequencing analyses, the S. chartarum extract was purified by ion exchange chromatography prior to in-gel alkylation and digestion with modified trypsin. RESULTS: @entity1 IgE reactivity was detected against eight components in the S. chartarum extract. Over 80% of the sera from the exposed subjects and less than 50% of the control sera recognized the 33-, 48- and 50-kD S. chartarum components. The @entity1 sera detected a 48- to 50-kD component from the extracts of eight fungal species. Nineteen peptide sequences were identified from the 48-kD component of S. chartarum. An analysis of the peptide sequences revealed homology with known fungal glycoside hydrolase enzymes (cellulases). CONCLUSIONS: The data showed @entity1 IgE reactivity against several S. chartarum components, including one at 48 kD. On the other hand, the @entity1 sera recognized 48- to 50-kD components from seven other fungal species, suggesting shared antigenic components (e.g. enolase) between the fungi. Thus, to our knowledge, this is the first antigen identified from S. chartarum.
