Molecular characterization and transcriptional regulation by GH and XXXX of insulin-like growth factors I and II in white seabream (Diplodus sargus).

@entity12067 @entity442 @entity5100 @entity1176 @entity5448 @entity10354 @entity6863
UNASSIGNED: @entity12067 are key regulators of development, growth and reproduction in fish. In the present study we have cloned and characterized the cDNA and genomic sequences of @entity442 and @entity5100 in the white seabream (Diplodus sargus). The @entity442 and @entity5100 genes were encoded putatively by five and four exons, respectively. Moreover, the 5'-flanking upstream region of the @entity442 gene contained highly conserved regulatory elements including @entity5448 , @entity10354 , @entity6863 ( @entity6863 ) and the TATA box. The full-length cDNAs were 1225 and 1666 nucleotides long for @entity442 and @entity5100 , respectively. Sequence analysis identified the A-E domains as well as three spliced forms involving the E domain in exons 3-5. ORF identities were higher than 83% with respect to other fish orthologs. Expression analysis demonstrated that @entity442 and its spliced forms were mostly expressed in liver, whereas the @entity5100 was expressed ubiquitously not detecting significant differences among the ten tissues analyzed. Hormonal treatments using the porcine GH demonstrated a sharply increase of both @entity442 and @entity5100 mRNA levels in liver and gills at 30min and 1h after injection. In the gonads, @entity442 mRNA levels increased steadily with testis and ovary maturation. In contrast, @entity5100 transcript amounts were higher in immature stages (S1-S2). Hormonal treatments using GH and @entity1176 demonstrated that @entity442 and @entity5100 expression were upregulated in the gonads. Overall, these data demonstrate that @entity442 and @entity5100 are locally expressed in several tissues and regulated by key hormones of the somatotropic and gonadotropic axes.
