Localization of lead in the kidney and liver of XXXX treated in vivo with @entity10222 : ultrastructural studies on unstained sections.

@entity13101 @entity10222 @entity3584 @entity409 @entity35 @entity10781
The subcellular distribution and ultrastructural effects of lead have been studied in the kidneys and liver of @entity35 given @entity10222 (600 ppm @entity10781 ) in their drinking water for 6 months. Control @entity35 were given @entity13101 . Tissue samples were fixed in @entity3584 and examined by electron microscopy with and without staining. Unstained sections of both kidney and liver from lead-treated animals showed small particles (2-5 nm diameter) of very high electron density which appear to represent a deposited form of @entity10781 . Ultrastructural changes in the kidneys were largely confined to glomeruli (swelling of endothelial cells, fusion of foot processes, thickening of basement membrane) and proximal tubules (ranging from minimal sub-lethal changes to necrotic disorganization). The electron-dense particles of @entity10781 occurred in large clusters in basement membranes. As individual particles, or small groups, they were numerous in nuclei of proximal epithelium but usually only a few, largely confined to vesicles or inclusion bodies, were present in the cytoplasm. Only when cells were markedly damaged morphologically were particles more generally distributed in the cytoplasm. @entity409 by @entity10781 was largely confined to centrilobular regions. Endothelial and Kupffer cells were the most affected; they often sequestered large numbers of the particles. In parenchymal cells, particles were few and mainly in vesicles, but they were more widely distributed in the cytoplasm when morphological injury was apparent. The free distribution of @entity10781 in liver and kidney seems to be limited by its deposition in basement membranes and sequestration in reticulo-endothelial cells; intracellular distribution in healthy cells is also limited, by deposition in nuclei (in kidney only) or cytoplasmic vesicles.
