Multilevel evaluations of potential @entity99 of XXXX .

@entity137 @entity19 @entity409 @entity2041 @entity312 @entity99 @entity9687
The widespread use of pesticides, such as @entity312 , increases health risks to non-target organisms. The potential @entity137 of @entity312 to the liver remains unclear and could be easily overlooked if only the common clinical indicators of @entity409 are examined. In the present study, BALB/c @entity19 were given intraperitoneal injections of 0, 2, 4, or 8   mg/kg @entity9687 (BF) for 7 days. The potential @entity99 of BF and its underlying mechanism were then investigated through multilevel evaluations. Histological analyses and serum enzyme activities showed no obvious clinical evidence of @entity409 . Oxidative stress was induced and caspases were activated in response to increased BF concentrations. Exposure to BF also significantly altered the expression levels of mitochondrial apoptosis-related genes in dose-dependent relationships. The microarray results showed that BF could disturb the metabolic profile and extensively induce genes related to oxidative stress, including the cytochrome P450 family, @entity2041 peroxidases, @entity2041 s-transferases and kinases. In the in vivo model, BF induced @entity99 through caspase-mediated mitochondrial-dependent cell death, a process that is closely related to oxidative stress, even in the absence of classical clinical biomarkers of @entity409 . The results of this study suggest that classical evaluations are not adequate for liver @entity137 of @entity312 , and highlight the need for more comprehensive assessment of health risks of these widely used pesticides.
