Different biological properties of circulating and bone marrow endothelial progenitor cells in acute myocardial infarction XXXX .

@entity35 @entity296 @entity63
BACKGROUND: Many studies have shown that endothelial progenitor cell (EPC) can enhance the neovascularization of the @entity63 myocardium. Peripheral blood and bone marrow are the most convenient resources for EPC. OBJECTIVE: The aim of the study was to investigate, in vitro and in vivo, the different biological properties between circulating EPC (CEPC) and bone marrow EPC (BM-EPC) of AMI @entity35 . METHODS: The proliferative, migrative, adherent and angiogenic properties were investigated in vitro. 1 x 10 (6) CEPCs, 1 x 10 (6) BM-EPCs and medium (EBM-2) were injected in the myocardium of AMI @entity35 . Echocardiography, @entity296 ( @entity296 ), capillary density and Y chromosome fluorescence in situ hybridization (FISH) were performed at 4 weeks after transplantation. RESULTS: The CEPCs had higher proliferative, migrative, adherent capabilities and lower senescent ratio, could adhere more quickly to fibronectin than BM-EPCs. CEPCs could form capillary-like structures whereas BM-EPCs did not show similar structures on Matrigel. in vivo, transplanted CEPCs and BM-EPCs were found in cardiac tissue by FISH. CEPCs treatment led to a better cardiac function, @entity296 and capillary density than BM-EPCs. CONCLUSION: Different biological properties were observed between CEPCs and BM-EPCs. Autologous CEPCs are more suitable for the AMI @entity35 .
