Expression of the @entity517 proto-oncogenes in developing XXXX fetal brain.

@entity1 @entity4180 @entity517 @entity10005
We have analysed @entity517 , @entity4180 and @entity10005 gene expression in developing @entity1 fetal brain by Northern hybridization, RNAase protection and in situ hybridization. The unique zonal organization of the developing fetal brain allows a particularly good assessment of the coupling of @entity517 gene expression to cell proliferation and differentiation in vivo. By Northern and in situ hybridization, @entity10005 as well as @entity517 and @entity4180 transcripts in the brain were found in the post-mitotic cortical and intermediate layers, as well as in the mitotically active layers containing the neuroepithelial precursor cells. Consistent results were also obtained for @entity10005 using RNAase protection analysis. Both the 3.6 and 3.8kb forms of the @entity10005 mRNA, resulting from alternative splicing of intron I, were detected in layers of neuroectodermal origin, but not in the meninges or choroid plexus. We also extended @entity10005 expression and splicing analyses to other developing @entity1 fetal tissues. @entity10005 mRNA was expressed in several other fetal tissues, particularly in fetal skin. Predominantly intron I containing @entity10005 mRNA was observed in fetal striated and cardiac muscle. Thus, @entity10005 is expressed in a wider spectrum of developing tissues than previously known. Our findings also, show that @entity10005 as well as @entity4180 and @entity517 expression is uncoupled from cell division in developing brain.
