Differentiated transplant derived airway epithelial cell cytokine secretion is not regulated by XXXX .

@entity1 @entity32 @entity650 @entity82 @entity1356 @entity310 @entity5962 @entity205 @entity358 @entity2533 @entity4154 @entity687
BACKGROUND: While lung transplantation is an increasingly utilized therapy for advanced @entity358 , chronic rejection in the form of @entity4154 ( @entity4154 ) continues to result in significant allograft dysfunction and @entity1 mortality. Despite correlation of clinical events with eventual development of @entity4154 , the causative pathophysiology remains unknown. Airway epithelial cells within the region of @entity32 and @entity82 associated with @entity4154 may have a participatory role. METHODS: Transplant derived airway epithelial cells differentiated in air liquid interface culture were treated with @entity1356 and/or @entity205 , after which secretion of cytokines and growth factor and gene expression for markers of epithelial to mesenchymal transition were analyzed. RESULTS: Secretion of @entity650 , @entity687 , and @entity310 , but not @entity2533 , was increased by @entity1356 stimulation. In contrast to previous studies using epithelial cells grown in submersion culture, treatment of differentiated cells in ALI culture with @entity205 did not elicit cytokine or growth factor secretion, and did not alter @entity650 , @entity687 , or @entity310 production in response to @entity1356 treatment. Neither @entity1356 nor @entity205 elicited expression of markers of the epithelial to mesenchymal transition @entity5962 , EDN-fibronectin, and a-smooth muscle actin. CONCLUSION: Transplant derived differentiated airway epithelial cell @entity650 , @entity687 , and @entity310 secretion is not regulated by @entity205 in vitro; these cells thus may participate in local inflammatory responses in the setting of immunosuppression. Further, treatment with @entity1356 did not elicit gene expression of markers of epithelial to mesenchymal transition. These data present a model of differentiated airway epithelial cells that may be useful in understanding epithelial participation in @entity32 and allograft rejection in lung transplantation.
