Extracellular vesicles derived from XXXX stem cells induce a pro-tumorigenic phenotype in mesenchymal stromal cells.

@entity3335 @entity5 @entity703 @entity4112 @entity19787 @entity1096 @entity3 @entity687 @entity436
@entity703 have been shown to contain a population of @entity5 stem cells (CSCs) that present self-renewing capacity and support @entity5 growth and @entity3 . CSCs were shown to secrete large amount of extracellular vesicles (EVs) that can transfer several molecules (proteins, lipids and nucleic acids) and induce epigenetic changes in target cells. Mesenchymal Stromal Cells (MSCs) are susceptible to @entity5 signalling and can be recruited to @entity5 regions. The precise role of MSCs in @entity5 development is still under debate since both pro- and anti-tumorigenic effects have been reported. In this study we analysed the participation of renal CSC-derived EVs in the interaction between @entity5 and MSCs. We found that CSC-derived EVs promoted persistent phenotypical changes in MSCs characterized by an increased expression of genes associated with cell migration ( @entity436 , @entity4112 ), matrix remodeling ( @entity19787 ), angiogenesis and @entity5 growth ( @entity687 , @entity1096 and @entity3335 ). EV-stimulated MSCs exhibited in vitro an enhancement of migration toward the @entity5 conditioned medium. Moreover, EV-stimulated MSCs enhanced migration of @entity703 cells and induced vessel-like formation. In vivo, EV-stimulated MSCs supported @entity5 development and vascularization, when co-injected with @entity703 cells. In conclusion, CSC-derived EVs induced phenotypical changes in MSCs that are associated with @entity5 growth.
