Identification of genes expressed in response to light stress in leaves of XXXX using RNA differential display.

@entity1 @entity958 @entity1457 @entity70601 @entity7692 @entity42642 @entity12234 @entity70600
The plant cell responds to light stress by the expression of genes encoding specific stress proteins with possible protective functions. Five genes, the mRNA levels of which increased drastically in response to light stress in mature green leaves of @entity958 were identified and isolated by the differential display technique. These genes were designated @entity7692 - @entity70600 (light stress-regulated). Northern blot analysis demonstrated that the transcript level of @entity7692 - @entity70600 increased 4- to 17-fold under light stress conditions as compared with leaves incubated at low intensity light. Further analysis of the @entity7692 - @entity70600 transcript level under cold stress, @entity1457 , wounding, desiccation, salt stress, oxidative stress and UV-A irradiation showed that the expression of all five genes was triggered by more than one stress factor. Thus, it was expected that isolated genes encode proteins involved in general stress responses. Homology searches revealed that all of the isolated cDNAs were represented in the GenBank in genomic DNAs and expressed sequence tag (EST) cDNA clones. The @entity7692 - @entity12234 genes encoded cytoplasmic proteins with assigned identities, such as @entity7692 (early responsive to dehydration), @entity42642 ( @entity42642 ), @entity70601 (late embryogenesis abundant) and @entity12234 (metallothionein class 1a), respectively. Light stress had not yet been reported to induce or enhance the expression of these genes. The @entity70600 clone encoded a novel protein with high similarity to beta-1,3-galactosyltransferases from @entity1 and primates predicted to be located in the Golgi body. Three ORFs homologous to the @entity70600 gene were found on chromosome I and IV of @entity958 indicating that a multigene family of these proteins exists in plants. The possible role of Lsr gene products in light stress defences is discussed.
