Urmylation: a @entity8290 -like pathway that functions during @entity851 and budding in XXXX .

@entity46279 @entity851 @entity70918 @entity3446 @entity70920 @entity70919 @entity23264 @entity70922 @entity375 @entity8290 @entity70921
@entity8290 is a small modifier protein that is conjugated to substrates to target them for degradation. Recently, a surprising number of @entity8290 -like proteins have been identified that also can be attached to proteins. Herein, we identify two molecular functions for the posttranslational protein modifier from @entity375 , @entity70918 . Simultaneous loss of @entity70918 and @entity46279 , a p21-activated kinase that functions in budding, is lethal. This result suggests a role for the urmylation pathway in budding. Furthermore, loss of the urmylation pathway causes defects in @entity851 and confers sensitivity to @entity3446 . Our results indicate that the sensitivity to @entity3446 is due to a genetic interaction with the TOR pathway, which is important for regulation of cell growth in response to nutrients. We have found that @entity70918 can be attached to a number of proteins. Loss of five genes that are also essential in a cla4Delta strain, @entity70919 , @entity70920 , @entity70921 , @entity70922 , and @entity23264 , affect the level of at least one @entity70918 conjugate. Moreover, these five genes have a role in @entity851 and display genetic interactions with the TOR pathway. In summary, our results suggest the urmylation pathway is involved in nutrient sensing and budding.
