Immunohistochemical studies on the proliferation of reactive astrocytes and the expression of cytoskeletal proteins following XXXX in @entity35 .

@entity1545 @entity1603 @entity665 @entity40 @entity35 @entity7229
The appearance of reactive astrocytes following @entity1545 was investigated in 4-week-old @entity35 with special reference to their proliferation and chronological changes in the cytoskeletal proteins. Two days after the injury, @entity40 ( @entity40 )-positive cells had increased in number around the lesion and spread to the entire ipsilateral cortex by 3 days after the injury. To investigate the distribution of mitotic cells and its chronological change, immunohistochemical staining with monoclonal antibody to @entity1603 ( @entity1603 ) was performed. @entity1603 -positive cells began to appear around the lesion and spread to the entire ipsilateral cortex by 3 days and their distribution was the same as that of @entity40 -positive cells. To investigate the association of @entity40 -positive cells with cell division, double labeling experiments using @entity665 autoradiography and immunohistochemical staining with antiserum to @entity40 were performed. Cells doubly labeled with @entity40 and @entity665 were localized in the area adjacent to the lesion, in the molecular layer of the cortex and in the white matter. By contrast, none of the cells were doubly labeled in the IInd to VIth layers of the cortex. Furthermore, only astrocytes in the former areas expressed @entity7229 transiently from 2 to 10 days after the injury. In the @entity35 administered vincristine, cells arrested during mitosis were found in the regions which express @entity7229 . From these results, it was suggested that astrocytes in the molecular layer of the cortex and the white matter adjacent to the lesion proliferated in response to the injury and expressed @entity7229 transiently, then acquired @entity40 , and that astrocytes in the IInd to VIth layers of the cortex became reactive astrocytes without mitosis.
