A new modified animal model of XXXX -induced experimental autoimmune @entity5442 enhanced by defibrase.

@entity1 @entity1707 @entity2002 @entity32 @entity5442 @entity39 @entity908 @entity840 @entity95 @entity425 @entity3999 @entity3795 @entity6558
INTRODUCTION: We investigated the effect of defibrase (a proteolytic enzyme extraction of Agkistrodon halys venom) on experimental autoimmune @entity5442 (EAM) in @entity840 and explored the option of using a modified @entity1707 model of EAM to enhance the study of this disease. MATERIAL AND METHODS: @entity840 were divided into 3 groups: group A (control group) was immunized with complete Freund adjuvant ( @entity3795 ), then received 6 injections of saline weekly; group B (EAM group) was immunized with partially purified @entity95 @entity3999 emulsified with @entity3795 , then received an injection of saline; group C (EAM + defibrase group) was immunized with purified @entity95 @entity3999 emulsified with @entity3795 , then received an injection of defibrase. The animals were observed for their general health condition and the body weight was measured daily. Plasma levels of @entity2002 and @entity908 kinase (CK) were determined. Muscle tissues were examined histologically. RESULTS: After immunizations for 6 weeks, incidence of EAM in groups A, B and C was 0 (0/7), 83.3% (10/12) and 100% (15/15), respectively. @entity840 with EAM presented angeitis symptoms of @entity425 . Histological analysis revealed a significant difference. Muscles with EAM had scattered or diffuse inflammatory manifestations, which are also common pathological features of @entity1 @entity6558 ( @entity6558 ). Defibrase-treated animals displayed extensive @entity32 and fiber @entity39 compared with the EAM group (histological score: 2.80   1.15 vs. 1.88   1.32, p < 0.05). Severity of @entity32 of group B was mainly mild to moderate; 16.7% (2/12) of animals developed severe @entity32 . Incidence of severe @entity32 with a score up to 4 in group C was 40% (6/15). CONCLUSIONS: Defibrase can exacerbate @entity3999 -induced EAM; thus a new modified model was generated.
