Acute Cutaneous Wounds Treated with XXXX Decellularised Dermis Show Enhanced Angiogenesis during Healing.

@entity1 @entity22079 @entity16943 @entity6061 @entity7672
BACKGROUND: The influence of skin substitutes upon angiogenesis during wound healing is unclear. OBJECTIVES: To compare the angiogenic response in acute cutaneous @entity1 wounds treated with autogenic, allogenic and xenogenic skin substitutes to those left to heal by secondary intention. METHODS: On day 0, four 5mm full-thickness punch biopsies were harvested from fifty healthy volunteers (sites 1-4). In all cases, site 1 healed by secondary intention (control), site 2 was treated with collagen-GAG scaffold (CG), cadaveric decellularised dermis (DCD) was applied to site 3, whilst excised tissue was re-inserted into site 4 (autograft). Depending on study group allocation, healing tissue from sites 1-4 was excised on day 7, 14, 21 or 28. All specimens were bisected, with half used in histological and immunohistochemical evaluation whilst extracted RNA from the remainder enabled whole genome microarrays and qRT-PCR of highlighted angiogenesis-related genes. All wounds were serially imaged over 6 weeks using laser-doppler imaging and spectrophotometric intracutaneous analysis. RESULTS: Inherent structural differences between skin substitutes influenced the distribution and organisation of capillary networks within regenerating dermis. Haemoglobin flux (p = 0.0035), @entity7672 concentration (p = 0.0005), and vessel number derived from CD31-based immunohistochemistry (p = 0.046) were significantly greater in DCD wounds at later time points. This correlated with time-matched increases in mRNA expression of @entity22079 ( @entity22079 ) (p = 0.021) and @entity16943 ( @entity16943 ) (p = 0.004). CONCLUSION: Corroborating evidence from invasive and non-invasive modalities demonstrated that treatment with @entity6061 resulted in increased angiogenesis after wounding. Significantly elevated mRNA expression of pro-angiogenic @entity16943 and extracellular matrix protease @entity22079 seen only in the DCD group may contribute to observed responses.
