The effect of XXXX on isolated rodent islets transplanted to the kidney capsule site.

@entity6 @entity3839 @entity4461 @entity35 @entity4854
The effect of @entity4854 on transplanted @entity35 pancreatic islets was studied using a new technique for transplanting a defined number of islets in a @entity4461 . Normal or @entity3839 - @entity6 DA @entity35 were given 400 DA islets under the left kidney capsule (a number shown to be insufficient to reverse @entity6 ). After 2 weeks the @entity6 @entity35 were given a further 1000 islets under the right kidney capsule to reverse @entity6 . Kidneys from both groups were examined at 2 weeks after the initial islet implant and at 3 months for both gross and histological appearance and for insulin content. After 2 weeks left kidneys from nondiabetic @entity35 showed abundant islet tissue, with an insulin content of 116 (+/- 14 SEM) milliunits, compared to 1 +/- 0.5 milliunits in the right kidney. Kidneys from @entity6 @entity35 showed no islets recognizable grossly. Histological examination showed vacuolated tissue scarcely recognizable as islet tissue, and the insulin content of the left kidney was reduced to 18 +/- 5 milliunits. However, 3 months after reversal of @entity6 by transplantation of 1000 islets to the right kidney, histologically "normal" islet tissue was again visible on the left kidney, and the insulin content was 160 +/- 36 milliunits. Islets left in normal animals for 3 months contained 195 +/- 50 milliunits. These experiments show that islets implanted beneath the kidney capsule in @entity6 @entity35 are not destroyed by two weeks @entity4854 . This suggests that protection of islets after implantation by insulin treatment is unnecessary, even in the presence of a persistently raised blood sugar.
