Expression of melanoma antigen gene by cells from inflamed joints in XXXX .

@entity1 @entity404 @entity5750 @entity5 @entity6472 @entity792 @entity309
OBJECTIVE: To determine if synovial fluid (SF) cells from inflamed joints of @entity1 with @entity309 ( @entity309 ) express melanoma antigen gene (MAGE) RNA and protein. METHODS: The pattern of @entity6472 expression was analyzed in inflammatory synovial tissue and peripheral blood mononuclear cells (PBMC) from @entity1 with @entity309 by immunocytochemistry, reverse transcription-polymerase chain reaction (RT-PCR), and flow cytometry. RESULTS: @entity6472 , previously detected only in @entity5 cells, is strongly expressed in SF cells from @entity1 with @entity309 . Immunocytochemistry revealed strong staining of SF cells in all of 22 specimens tested. PBMC from @entity1 (7 of 7) also expressed @entity6472 , but not as strongly as SF cells. Two-color immunofluorescence showed colocalization with @entity404 and @entity5750 . Flow cytometry on 3 samples of SF cells confirmed the presence of @entity6472 on the cell surface and intracellularly. Five of 5 SF cell samples were positive for @entity6472 by RT-PCR. CONCLUSION: Mononuclear cells from inflamed joints and blood from @entity1 with @entity309 express @entity6472 . MAGE family proteins were previously thought to be expressed only by certain @entity5 and presented by HLA Class I, resulting in @entity5 cell lysis by cytotoxic T cells. The observation of @entity6472 expression in @entity309 suggests an association with @entity792 and a possible causal role for MAGE antigen in the chronic inflammation of @entity309 .
