Rapid diagnosis of XXXX by means of a dot hybridization assay.

@entity19 @entity3971 @entity281 @entity17839 @entity521 @entity204
Dot hybridization was used to detect specific rabies RNA in brains, either from experimental @entity281 in @entity19 or from brain material to be processed for routine diagnosis. 32P cDNA probes were employed to identify minute amounts of specific viral RNA. Purified RNA was obtained after @entity3971 extraction. The RNA was fixed on nylon membranes and hybridized with a pool of M13 inserts complementary to 200-400 nucleotides of each rabies gene and mRNA. Hybridized, labelled probes were detected by autoradiography. There was strong cross-hybridization between fixed rabies and street @entity17839 RNA, which enable the detection of field strains for diagnosis purpose using a fixed rabies (PV strain) cDNA. A positive response was obtained with as little as 80 ng of brain RNA material from a fixed @entity281 @entity19 . Detection of viral RNA was still specific 1 week after @entity204 , the brain material being left at room temperature. A total correlation was found when the samples were examined in parallel using a fluorescent rabies-specific antibody and by virus isolation on @entity19 @entity521 cells. These data show that the use of rabies-specific cDNA probes in a dot-blot hybridization assay has great potential for the diagnosis of rabies.
