Application of immunogold labelling for light and electron microscopic localization of @entity4105 ( XXXX ) on cultured @entity1 endothelial cells.

@entity1 @entity39 @entity310 @entity5 @entity575 @entity990 @entity4515 @entity1579 @entity4621 @entity4105
This study describes the expression characteristics of @entity4105 molecules using immunogold histochemical techniques on cultured @entity1 umbilical vein endothelial cells (HUVEC). The expression of @entity4105 was induced by @entity5 @entity39 factor-alpha ( @entity310 , 300 U/ml), @entity4621 ( @entity4515 , 10 ng/ml) and bacterial lipopolysaccharide (LPS, 4 micrograms/ml). No expression was demonstrated on control cells. Using the @entity575 -enhanced colloidal gold-labelling technique, at the light microscopical level, HUVEC could be distinctively subdivided into three staining types. The cell labelling index, expressed as the number of 'positively' stained cells as a proportion of all viewed cells was the highest in the LPS group. For @entity1579 ( @entity1579 ) the preembedding immunocytochemical staining method and embedding in @entity990 resin (Agar 100) according to standard procedures was used. In @entity1579 gold particles were localized in close association with the apical plasma membrane, as well as on the surface of microvillus-like projections (the latter by @entity310 group). For high resolution scanning electron microscopy (HR-SEM) the secondary (SEI) and the backscattered electron imaging (BEI) modes were used. Gold particles were randomly distributed over the whole cell surface, although they appeared to be denser in the perinuclear zone. The quantitative evaluation on SE and BE viewing (the number of gold particles per cell area in microns 2) demonstrated the highest density of labelling in the LPS-treated group, but there was only a significant difference between LPS and @entity310 groups (P < 0.01, t-test). Furthermore, the ultrastructural studies indicated that treatment with substances which up-regulate @entity4105 expression was not related to toxic cell damage or significant alterations of cellular ultrastructure.
