XXXX levels in the supernatant of conditioned media of embryos grown in autologous endometrial coculture: correlation with outcome after @entity52 .

@entity1 @entity1356 @entity7288 @entity1193 @entity1192 @entity52
PROBLEM: To determine if @entity1192 produced by autologous endometrial coculture (AECC) was associated with outcome in @entity1 with a history of multiple @entity52 ( @entity52 ) failures. METHOD OF STUDY: The conditioned media (CM) from AECC cells exposed or non-exposed to @entity1 embryos was analyzed for IL-1. RESULTS: Embryos grown on AECC demonstrated a significant improvement in number of blastomeres and fragmentation (frag) when compared to embryos grown in conventional media without ECC (6.4 +/- 1.3 vs. 5.5 +/- 1.2 blastomeres and 14.6 +/- 9.3%, vs. 18.4 +/- 9.8% frag; P< 0.008 and 0.003, respectively). When @entity1192 and @entity1356 were undetectable in the CM, the embryos grown in ECC were of improved quality as compared to the embryos grown only in conventional media. Conversely, @entity1193 levels in the CM were positively associated with embryo quality. Exposure or non-exposure to an embryo did not result in differing levels of @entity1192 , @entity1356 , or @entity1193 in the CM. @entity1356 levels were negatively associated with clinical pregnancy outcome (3.3 pg/mL (pregnant, n = 12) vs. 27.1 pg/mL (not pregnant, n = 17); P = 0.008, Mann Whitney U-test). @entity1192 and @entity1193 levels were not associated with outcome. CONCLUSIONS: We have demonstrated a significant improvement in blastomere number and frag with ECC. The presence of @entity1356 in the CM was negatively associated with embryonic development and clinical pregnancy. The presence of @entity1192 in the CM was negatively associated with embryonic development and the presence of @entity1193 in the CM was positively associated with embryonic development. Whether @entity1356 itself interferes with successful outcome after embryo transfer or if it is a marker for undetected @entity7288 in the biopsy specimens remains to be determined.
