[Influence of mutation of XXXX of COL I A1 gene on the biochemical function of osteoblast].

@entity1 @entity22219 @entity1555 @entity460 @entity731
OBJECTIVE: To study the influence of mutation of @entity22219 of COL I A1 gene on the biochemical function of osteoblast, and the pathomechanism of @entity1555 . METHODS: Spongy bones were obtained to culture osteoblast primarily during total hip or knee replacements. The genotype of osteoblast was identified with PCR-RFLP. The amount of mRNA of COL I A1 and collagen type I were determined by RT-PCR and ELISA. The growth of osteoblast, the activity of bone @entity731 , and the amount of @entity460 in cell matrix and @entity460 nodus were measured. RESULTS: Three genotypes GG, GT and TT in osteoblast were successfully identified. Compared with GG and GT genotypes, lower expression of mRNA of COL I A1 gene, lesser collagen type I, @entity460 in cell matrix, and @entity460 nodus were found in the cells with TT genotype (P < 0.01). No significant differences were found between GG and GT genotype (P > 0.05). There were no significant differences in age, growth of osteoblast, and activity of bone @entity731 among the three genotypes (P > 0.05). CONCLUSION: Cells with TT genotype have low expression of mRNA of COL I A1 gene and less collagen type I , @entity460 in cell matrix and @entity460 nodus. The lower amount of collagen type I synthesized by osteoblast can decrease the matrix outside the bone cells and result in insufficient site for @entity460 deposition. This may be the cause of lower @entity1555 in @entity1 with TT genotype.
