Protein A immobilized polyhydroxyethylmethacrylate beads for affinity sorption of XXXX immunoglobulin G.

@entity1 @entity26337
Protein A immobilized @entity26337 ( @entity26337 ) microbeads were investigated for the specific removal of HIgG from aqueous solutions and from @entity1 plasma. @entity26337 microbeads were prepared by a suspension polymerization technique and activated by CNBr in an alkaline medium (pH 11.5). Protein A was then immobilized by covalent binding onto these microbeads. The amount of immobilized protein A was controlled by changing pH and the initial concentrations of CNBr and protein A. The maximum protein A immobilization was observed at pH 9.5. Up to 3.5 mg protein A/g @entity26337 was immobilized on the CNBr activated @entity26337 microbeads. The maximum HIgG adsorption on the protein A immobilized @entity26337 microbeads was observed at pH 8.0. The non-specific HIgG adsorption onto the plain @entity26337 microbeads was low (about 0.167 mg of HIgG/g @entity26337 ). Higher adsorption values (up to 6.0 mg of HIgG/g @entity26337 ) were obtained in which the protein A immobilized @entity26337 microbeads were used. Much higher amounts of HIgG (up to 24.0 mg of HIgG/g @entity26337 ) were adsorbed from @entity1 plasma.
