Cytokine, infiltrating macrophage and T cell-mediated response to development of primary and secondary XXXX liver cancer.

@entity1 @entity39 @entity5 @entity1356 @entity469 @entity409 @entity3 @entity157
BACKGROUND: Kupffer cells, monocytes and infiltrating T cells have been considered the major source of @entity1356 and @entity5 @entity39 factor-alpha in the liver. AIMS; To explore the expression of @entity1356 and @entity5 @entity39 factor-alpha and to evaluate the density and the distribution of T lymphocytes and monocytes/macrophages in the liver of @entity1 with primary and secondary @entity5 . METHODS: Tumoural and peritumoural liver samples were examined from 21 @entity1 with @entity157 , 10 with hepatic @entity3 , 5 with benign focal liver lesions and 4 healthy adult livers. @entity1356 and @entity5 @entity39 factor-alpha mRNAs were detected by a semiquantitative comparative reverse transcriptase polymerase chain reaction. T lymphocytes and monocytes/macrophages were detected by immunohistochemistry. RESULTS: Higher levels of @entity1356 , @entity5 @entity39 factor-alpha, CD3+ and @entity469 + cells were found in the tissue surrounding @entity157 and @entity3 than in the @entity5 itself. A strong expression of @entity469 + and CD3+ cells was found mainly along the @entity5 -host interface but the highest expression of CD3+ cells was found at the metastasis interfaces. @entity1356 expression, CD3+ and @entity469 + cell densities were higher in peritumoural samples than in so-called "normal" liver tissue. CONCLUSIONS: An increased production of @entity1356 and, to a lesser extent, of @entity5 @entity39 factor-alpha mRNA coincides with the presence of @entity5 be it primary or secondary, both in healthy and cirrhotic livers. The presence of @entity5 , irrespective of the presence of underlying @entity409 , appears to play the most important role.
