Increased @entity934 release in XXXX from allergen-exposed IgE-immune @entity19 .

@entity825 @entity19 @entity1617 @entity1391 @entity2710 @entity934
Increased release of @entity934 ( @entity1617 ) from airway parasympathetic nerve endings is one mechanism that may contribute to increases in airway responsiveness in immunoglobulin E (IgE)-immune allergen-exposed animals. We measured @entity1617 released from @entity19 @entity825 following electrical field stimulation in vitro. BALB/c @entity19 were immunized by exposure to an aerosol of 1% ovalbumin in sterile @entity1391 -buffered saline for 20 min/day for 10 days. At this time, levels of ovalbumin-specific IgE were proportionately higher than ovalbumin-specific IgG. As a control, nonimmune @entity19 were similarly exposed to @entity1391 -buffered saline alone. Forty-eight hours after the last aerosol, @entity825 were removed for assessment of either the contractile responses to electrical field stimulation and a cholinergic agonist ( @entity2710 or @entity1617 ) or release of @entity1617 produced by electrical field stimulation. @entity1617 in the bath was measured using high-performance liquid chromatography with electrochemical detection. The stimulation frequencies causing one-half the maximal contractile response to electrical field stimulation were 4.1 +/- 0.2 and 2.8 +/- 0.2 Hz (P = 0.0001) for nonimmune and immune @entity19 , respectively, whereas the molar concentrations of @entity2710 causing one-half of the maximal contractile response did not significantly differ. In addition, the dose-response curves of immune and nonimmune @entity825 to @entity1617 were superimposable. A significant increase in @entity1617 release was demonstrated at both 10 and 20 Hz in @entity825 from immune @entity19 . @entity1617 release (pmol.g tissue-1.min-1) from nonimmune and immune @entity19 @entity825 , respectively, were 140 +/- 8 and 205 +/- 22 (P = 0.013) at 10 Hz and 147 +/- 13 and 227 +/- 14 (P = 0.008) at 20 Hz.(ABSTRACT TRUNCATED AT 250 WORDS)
