Silver nanoparticles of 70   nm and 20   nm affect differently the biology of XXXX neutrophils.

@entity1 @entity26 @entity358 @entity1495 @entity687
UNASSIGNED: The influence of size of nanoparticles (NP), especially in regard to @entity358 , has been widely investigated. In general, NP with smaller diameters are more pro-inflammatory in vivo, at least in terms of neutrophil influx. Nevertheless, the influence of size of NP on polymorphonuclear neutrophil (PMN) cell biology is poorly documented. In the study here, it was decided to determine if AgNP with a diameter of 70   nm (AgNP70) will alter the biology of @entity1 PMN similarly to AgNP20 previously reported to induce apoptosis and inhibit de novo protein synthesis. The results here indicated that, in contrast to AgNP20, AgNP70 delayed PMN apoptosis. However, both AgNP20 and AgNP70 inhibited de novo protein synthesis. Both forms of AgNP did not significantly increase reactive @entity26 species (ROS) production, but AgNP20 significantly increased the cell production of the @entity687 chemokine ( @entity687 ). In addition, AgNP20, but not AgNP70, induced the release of albumin and matrix metalloproteinase-9 ( @entity1495 /gelatinase B) into culture supernatants. Consistent with this latter observation, gelatinase activity was increased by AgNP20, as assessed by zymography. From these outcomes, it is concluded that two NP with different initial diameters can possess similar - as well as distinct - biological properties in modulating @entity1 PMN functions. These outcomes are testimony to the complexity of the modes of action of NP at the cellular level.
