Therapeutic impact of XXXX bone marrow stromal cells expanded by animal serum-free medium for @entity385 in @entity35 .

@entity1 @entity6220 @entity2500 @entity1188 @entity35 @entity385
BACKGROUND: The donor cell culture in animal serum-free medium is important for the clinical application of cell transplantation therapy. Recently, @entity1 -derived platelet lysate (PL) gained interest as a substitute for fetal calf serum (FCS), but there are no studies that evaluate the validity of @entity1 bone marrow stromal cells (hBMSCs) expanded with PL-containing medium for @entity6220 . OBJECTIVE: To test the hypothesis that hBMSCs expanded with FCS-free, PL-containing medium can promote functional recovery after @entity385 . METHODS: hBMSCs were cultured in the FCS- or PL-containing medium. Cell-growth kinetics were analyzed. The vehicle or hBMSCs was stereotactically transplanted into the ipsilateral striatum of the @entity35 subjected to permanent @entity2500 7 days after the insult. Motor function was assessed for 8 weeks, and the fate of transplanted hBMSCs was examined using immunohistochemistry. RESULTS: There was no significant difference in hBMSC expansion between the 2 groups. Transplantation of hBMSCs expanded with the FCS- or PL-containing medium equally promoted functional recovery compared with the vehicle group. Histological analysis revealed that there were no significant differences in their migration, survival, and neural differentiation in the @entity1188 brain between the 2 groups. CONCLUSION: hBMSCs expanded with PL-containing medium retained their capacity of migration, survival, and differentiation and significantly promoted functional recovery when stereotactically transplanted into the @entity1188 brain. The PL may be a clinically valuable and safe substitute for FCS in expanding hBMSCs to regenerate the @entity1188 brain.
