Cell surface P- and @entity4105 support shear-dependent rolling of XXXX gamma/delta T cells.

@entity1 @entity42087 @entity3167 @entity556 @entity27217 @entity4105 @entity8729 @entity53170 @entity291 @entity1098
The vascular selectins P- and @entity4105 are inducible adhesion proteins expressed by endothelial cells that have been shown to support shear-dependent rolling of myeloid cells. This interaction is thought to be a prerequisite event for subsequent steps, such as tight adhesion/aggregation and transendothelial cell migration, involved in the accumulation of leukocytes into tissues. Certain lymphocyte subsets have also been shown to bind the vascular selectins, but the importance of this interaction in mediating shear-dependent rolling, as described for myeloid cells, has not been demonstrated. We expand on our earlier observation that @entity291 gamma/delta T cells bind @entity4105 by showing that this interaction leads to a reproducible rolling event in assays done under shear forces that approximate those that occur in vivo. @entity4105 , expressed by L cell transfectants or cytokine-stimulated @entity1 and @entity291 endothelial cells, equally supports the shear-dependent rolling interaction. The lymphocyte adhesion proteins L-selectin, @entity27217 , and @entity42087 do not contribute to this event. @entity53170 treatment of the gamma/delta T cells or addition of @entity1098 to the assay completely blocks the rolling interaction. We further show for the first time that @entity8729 expressed by @entity556 -activated platelets or a soluble @entity8729 / @entity1 Ig chimera specifically binds gamma/delta T cells. The @entity8729 interaction is similar to the rolling event mediated by @entity4105 --it requires divalent cations and @entity3167 on the lymphocyte, it lacks involvement of L-selectin and @entity27217 , and rolling occurs under physiologic shear conditions. These results provide the documentation that the vascular selectins can support shear-dependent rolling of a lymphocyte subset and that @entity8729 mediates the adhesion of gamma/delta T cells.
