Attachment Ability of XXXX Apical Papilla Cells to Root Dentin Surfaces Treated with Either 3Mix or @entity6086 .

@entity1 @entity15383 @entity1358 @entity6086 @entity1412 @entity1098
INTRODUCTION: Tissue regeneration and root continuation are the ultimate goals in regenerative endodontics. @entity1358 ( @entity1358 ) have been hypothesized to play roles in those processes. Therefore, preservation of cell vitality and promoting initial cell attachment seem to be crucial steps. The purposes of this study were to investigate the attachment ability and morphology of viable @entity1 @entity1358 by using fibronectin immunofluoresence, alamarBlue assay, and scanning electron microscopy, when @entity1358 were grown on @entity1 root dentin surfaces treated with 3Mix or @entity6086 ( @entity15383 ) at different concentrations. METHODS: @entity1 root dentin slices were divided into 6 groups: (1) control, (2) 3Mix 0.39   g/mL, (3) 3Mix 100   g/mL, (4) 3Mix paste, (5) @entity15383 1  mg/mL, and (6) @entity15383 1000  mg/mL. All samples were separately treated for 7 days and final rinsed with 17% @entity1098 . Then primary @entity1 @entity1358 were seeded. Fibronectin immunofluorescence was used to evaluate the attachment ability of @entity1358 on treated dentin. A vitality assay by using alamarBlue was monitored at specific time intervals. The morphology of the cells on the dentin was observed under scanning electron microscopy. RESULTS: The lowest number of fibronectin-positive cells was observed on root dentin treated with 3Mix paste (P  <  .05). The 3Mix at 0.39 and 100   g/mL did not affect the amount of @entity1412 attachment, whereas the viability of @entity1358 on the dentin surface was significantly lower in the 100- g/mL 3Mix-treated group than in the negative control group (P  <  .05). Both concentrations of @entity15383 induced @entity1412 attachment (P  <  .05). Moreover, only cells grown on the surfaces treated with @entity15383 exhibited cytoplasmic processes. CONCLUSIONS: @entity1 root dentin treated with 3Mix paste had significantly lower @entity1412 attachment. The 3Mix at 0.39   g/mL and 100   g/mL had no significant negative effect on @entity1412 attachment on dentin. Higher numbers of cells attaching on dentin were observed in @entity6086 -treated groups.
