Localization of XXXX subtype mRNA in @entity35 kidney.

@entity35 @entity1809 @entity1808 @entity11154
The physiological effects of @entity1808 ( @entity1808 ) on the kidney are mediated primarily by the @entity1808 type 1 ( @entity1809 ) receptor. Two highly similar @entity1809 receptor subtypes have been identified in the @entity35 by molecular cloning techniques, namely @entity1809 and @entity11154 . The intrarenal localization of the @entity1809 and @entity11154 receptor subtypes has not been studied by hybridization methods with subtype-specific receptor probes. Using radiolabeled probes from the 3' noncoding region of the @entity1809 and @entity11154 cDNAs, we localized @entity1809 mRNA in @entity35 kidney by in situ hybridization. Specificity of the 3' noncoding region probes was tested by Northern blot and solution hybridization methods. @entity1809 mRNA levels were highest in the liver, kidney, and adrenal. In contrast, @entity11154 mRNA levels were highest in the adrenal and pituitary and low in kidney. Autoradiographic localization of 125I-[Sar1,Ile8] @entity1808 binding indicated that the highest levels of @entity1809 receptors were found in glomeruli and vascular elements. In situ hybridization with a nonselective @entity1809 receptor riboprobe indicated that the highest levels of @entity1809 mRNA were in the outer medullary vasa recta and cortical glomeruli with additional diffuse labeling of the cortex and outer medulla, consistent with labeling of tubular elements. In contrast, in situ hybridization with the @entity1809 subtype selective probes revealed that @entity1809 receptor mRNA was primarily localized to the vasa recta and diffusely to the outer stripe of the outer medulla and the renal cortex.(ABSTRACT TRUNCATED AT 250 WORDS)
