Significant effects of sex, strain, and anesthesia in the intrahippocampal XXXX @entity19 model of mesial @entity1315 .

@entity1545 @entity410 @entity131 @entity19 @entity4176 @entity1315 @entity302 @entity5161 @entity22292 @entity159
UNASSIGNED: The intrahippocampal @entity4176 @entity19 model of mesial @entity1315 is increasingly being used for studies on epileptogenesis and antiepileptogenesis. Almost all previous studies used male @entity19 for this purpose, and no study is available in this or other models of acquired @entity302 that directly compared epileptogenesis in female and male rodents. Epidemiological studies suggest that gender may affect susceptibility to @entity302 and its prognosis; therefore, one goal of this study was to investigate whether sex has an influence on latent period and epileptogenesis in the intrahippocampal @entity4176 model in @entity19 . Another aspect that was examined in the present study was whether @entity19 differences in epileptogenesis exist. Finally, we examined the effects of different types of anesthesia ( @entity22292 , @entity5161 ) on @entity4176 -induced @entity159 ( @entity159 ) and epileptogenesis. Continuous (24/7) video-EEG monitoring was used during @entity159 and the 2weeks following @entity159 as well as 4-6weeks after @entity159 . In male NMRI @entity19 with @entity22292 anesthesia during @entity4176 injection, @entity159 was followed by a @entity410 -free latent period of 10-14days if @entity131 ( @entity131 ) recorded from the @entity4176 focus were considered the onset of @entity302 . Anesthesia with @entity5161 led to a more rapid onset and higher severity of @entity159 , and not all male NMRI @entity19 exhibited a @entity410 -free latent period. Female NMRI @entity19 differed from male animals in the lack of any clear latent period, independently of anesthesia type. Furthermore, @entity131 were only rarely observed. These problems were not resolved by decreasing the dose of @entity4176 or using other strains (C57BL/6, FVB/N) of female @entity19 . The present data are the first to demonstrate marked sex-related differences in the latent period following @entity1545 in a rodent model of acquired @entity302 . Furthermore, our data demonstrate that the choice of anesthestic agent during @entity4176 administration affects @entity159 severity and as a consequence, the latent period, which may explain some of the differences reported for this model in the literature.
