Comparison of different types of stationary phases for the analysis of soy XXXX by HPLC.

@entity1042 @entity1068 @entity6400 @entity1626
Nowadays, there are new technologies in high-performance liquid chromatography columns available enabling faster and more efficient separations. In this work, we compared three different types of columns for the analysis of main soy @entity6400 . The evaluated columns were a conventional reverse phase particle column, a fused-core particle column, and a monolithic column. The comparison was in terms of chromatographic parameters such as resolution, asymmetry, number of theoretical plates, variability of retention time, and peak width. The lower column pressure was provided by the monolithic column, although lower chromatographic performance was achieved. Conventional and fused-core particle columns presented similar pressure. Results also indicate that direct transfer between particle and monolithic columns is not possible requiring adjustment of conditions and a different method optimization strategy. The best chromatographic performance and separation speed were observed for the fused-core particle column. Also, the effect of sample solvent on the separation and peak shape was evaluated and indicated that monolithic column is the most affected especially when using higher concentrations of @entity1626 or @entity1068 . Sample solvent that showed the lowest effect on the chromatographic performance of the columns was @entity1042 . Overall evaluation of @entity1042 and @entity1626 as mobile phase for the separation of @entity6400 indicated higher chromatographic performance of @entity1626 , although @entity1042 may be an attractive alternative. Using @entity1626 as mobile phase resulted in faster, higher resolution, narrower, and more symmetric peaks than @entity1042 with all columns. It also generated the lower column pressure and flatter pressure profile due to mobile phase changes, and therefore, it presents a higher potential to be explored for the development of faster separation methods.
