65-70 kD protein identified by immunoblotting as the presumptive gastric microsomal autoantigen in pernicious XXXX .

@entity1 @entity417 @entity235 @entity2631 @entity356 @entity3619 @entity309
Sera from 20 of 24 @entity1 with pernicious @entity417 reacted by immunoblotting with a 65-70 kD protein in @entity235 and rodent gastric mucosal cells enriched for 80-90% parietal cells, and in microsomal preparations derived from these cells. All 20 reactive sera were positive for parietal cell microsomal antibody demonstrated by immunofluorescence. Eighteen parietal cell microsomal antibody-positive sera from @entity1 with unconfirmed pernicious @entity417 also reacted with the same 65-70 kD protein. Serum reactivity with the same 65-70 kD protein was not seen with @entity235 and rodent liver cells or with microsomal preparations derived from these cells. Sera from 10 @entity1 with @entity3619 , 10 with @entity2631 and 10 with @entity309 and 22 healthy @entity1 did not react with the 65-70 kD protein. These results suggest that the 65-70 kD protein is probably the parietal cell microsomal autoantigen. A second antigen of 85-90 kD mol. wt. present only in @entity235 @entity356 also correlates with the presence of parietal cell microsomal antibody. However, the contribution of this second antigen to parietal cell microsomal antibody reactivity remains uncertain.
