Anticancer effects of fraction isolated from fruiting bodies of Chaga medicinal mushroom, Inonotus obliquus (Pers.:Fr.) XXXX  t (Aphyllophoromycetideae): in vitro studies.

@entity1 @entity1603 @entity137 @entity9057 @entity1819 @entity5 @entity19 @entity409 @entity2 @entity98 @entity35 @entity694 @entity291 @entity204
The medicinal mushroom Chaga, Inonotus obliquus (Pers.:Fr.) @entity9057  t (Hymenochaetaceae), has been used in folk medicine in Russia, Poland, and most of the Baltic countries, as a cleansing and disinfecting measure, and as decoctions for @entity1819 , @entity409 , and @entity5 treatment. Many reports have been published concerning the health promoting functions of this mushroom, including antibacterial, hepatoprotective, anti-inflammatory, antitumor, and antioxidant activities. The purpose of the present study was evaluation of in vitro anticancer activity of fraction IO4 isolated from I. obliquus. The effect on cell proliferation, motility and viability was assessed in a range of @entity5 and normal cells. Chaga fraction prepared from dried fruiting bodies was subjected to anticancer evaluation in @entity1 @entity2 (A549), @entity694 (HT-29), and @entity35 glioma (C6) cell cultures. @entity1 skin fibroblasts (HSF), @entity291 aorta endothelial cells (BAEC), models of @entity35 oligodendrocytes (OLN-93), hepatocytes (Fao), @entity35 astroglia, and @entity19 neurons (P19) were applied to test @entity137 in normal cells. The following methods were applied: @entity5 cell proliferation ( @entity98 assay and @entity1603 assay), @entity137 (LDH assay), @entity5 cell motility (wound assay), @entity5 cell morphology (May-Gr  nwald-Giemsa staining), and @entity204 detection (ELISA). Chaga fraction elicited anticancer effects which were attributed to decreased @entity5 cell proliferation, motility and morphological changes induction. Of note is the fact that it produced no or low @entity137 in tested normal cells. The data presented could open interesting paths for further investigations of fraction IO4 as a potential anticancer agent.
