XXXX over-expression phenotypes and @entity72631 gene molecular misreading during aging in @entity238 .

@entity1 @entity36725 @entity3403 @entity477 @entity72631 @entity179 @entity238
Molecular Misreading (MM) is the inaccurate conversion of genomic information into aberrant proteins. For example, when RNA polymerase II transcribes a GAGAG motif it synthesizes at low frequency RNA with a two-base deletion. If the deletion occurs in a coding region, translation will result in production of misframed proteins. During @entity1 aging, misframed versions of @entity1 @entity477 (hApp) and @entity72631 ( @entity36725 ) accumulate in the aggregates characteristic of @entity179 , suggesting dysfunctional degradation or clearance. Here cDNA clones encoding wild-type @entity36725 and the frame-shifted version @entity36725 (+1) were expressed in transgenic @entity238 using the @entity3403 -regulated system. Misframed proteins were abundantly produced, both from the transgenes and from endogenous @entity238 @entity72631 -encoding genes, and their abundance increased during aging in whole- @entity238 extracts. Over-expression of wild-type @entity36725 , but not @entity36725 (+1), was toxic during @entity238 development. In contrast, when over-expressed specifically in adult flies, @entity36725 (+1) caused small decreases in life span, whereas @entity36725 was associated with small increases, preferentially in males. The data suggest that MM occurs in @entity238 and that the resultant misframed proteins accumulate with age. MM of the @entity72631 gene can produce alternative @entity72631 gene products with different and sometimes opposing phenotypic effects.
