Periprostatic adipose tissue as a modulator of @entity263 XXXX .

@entity1 @entity650 @entity263 @entity146 @entity5 @entity4104
PURPOSE: Adipose tissue has been suggested to contribute to the pathogenesis of various disease states, including @entity263 . We investigated the association of cytokines and growth factors secreted by periprostatic adipose tissue with pathological features of @entity263 . MATERIALS AND METHODS: Periprostatic adipose tissue was harvested from @entity1 undergoing radical prostatectomy and cultured for 24 hours to generate conditioned medium or snap frozen immediately for functional signaling profiling. Multiplex analysis of the periprostatic adipose tissue conditioned medium was used to detect cytokine levels and compared to patient matched serum from 7 @entity1 . @entity650 in serum and periprostatic adipose tissue conditioned medium was further analyzed by enzyme-linked immunosorbent assay and correlated with clinical variables, such as age, body mass index and Gleason score, in 45 @entity1 . @entity650 expression in periprostatic adipose tissue was determined by immunohistochemistry. Reverse phase protein microarray technology was used to analyze cell signaling networks in periprostatic adipose tissue. RESULTS: @entity650 in periprostatic adipose tissue conditioned medium was approximately 375 times greater than that in patient matched serum and levels correlated with pathological grade. This finding was further extended by cell signaling analysis of periprostatic adipose tissue, which showed greater phosphorylation on @entity4104 with high grade @entity5 (any component of Gleason score 4 or 5). CONCLUSIONS: Higher Gleason score correlated with high levels of conditioned medium derived @entity650 . Moreover, cell signaling analysis of periprostatic adipose tissue identified activated signaling molecules, including @entity4104 , that correlated with Gleason score. Since @entity4104 is @entity650 regulated, these findings suggest that periprostatic adipose tissue may have a role in modulating @entity263 @entity146 by serving as a source of @entity650 . Also, we found low numbers of inflammatory cells in the fat, suggesting that adipocytes are the major secretors of @entity650 .
