Quantitative molecular grading of bladder tumours: a tool for objective assessment of the biological potential of XXXX .

@entity1 @entity2558 @entity4929 @entity1494 @entity5 @entity3162 @entity876 @entity1758
The present study aimed to assess whether @entity1 with bladder @entity5 can be more objectively stratified into low- and high-risk groups for recurrence and progression using a 2-tired molecular grading scheme, than by subjective histopathological grading alone. Biopsy material from 45 consecutive @entity1 with @entity1494 (2 @entity4929 of low malignant potentials, 18 pTa, 1 pTis, 19 @entity2558 ) was analysed for immunohistochemical Ki-67 and @entity876 expression. Labelling indices were assessed by automated cellular image analysis. UroVysion FISH test results were evaluated by automated signal counting, and DNA ploidy of single nuclei preparations were measured by image cytometry. Sixty-nine percent of cases showed >10% Ki-67 @entity3162 , 64% had >10% @entity876 @entity3162 , 53% revealed DNA aneuploidy and 56% expressed a high-risk FISH pattern. Based on a combination of single molecular markers, 75% of @entity5 were classified as high molecular grade. @entity5 stage and histopathological grade were significantly associated with FISH pattern, DNA ploidy and @entity1758 @entity3162 . Stage was also related with molecular grade. Clinical outcome showed a significant correlation with @entity1758 @entity3162 and molecular grade. @entity876 had neither diagnostic nor prognostic relevance, nor was there any correlation between histological and molecular grade. Our preliminary data strongly suggest that the combination of quantitative biomarkers provides superior and objective prognostic tools in bladder @entity5 compared to classic clinicopathological features and indices.
