Enzymes of XXXX metabolism in Frankia sp.

@entity7970 @entity2125 @entity1568 @entity3265 @entity16749 @entity4919 @entity6883 @entity1110 @entity14224 @entity12742 @entity413 @entity2388 @entity3776
Enzymes of @entity413 metabolism were assayed in crude cell extracts of Frankia strains HFPArI3 and HFPCcI2 as well as in isolated vesicle clusters from Alnus rubra root nodules. Activities of the Embden-Meyerhof-Parnas pathway enzymes @entity14224 , phosphofructokinase, and @entity2388 kinase were found in Frankia strain HFPArI3 and @entity14224 and @entity2388 kinase were found in Frankia strain HFPCcI2 and in the vesicle clusters. An @entity1110 +-linked @entity3776 dehydrogenase and an @entity2125 -linked @entity16749 dehydrogenase were found in all of the extracts, although the role of these enzymes is unclear. No @entity1110 +-linked @entity16749 dehydrogenase was found. Both dehydrogenases were inhibited by @entity6883 , and the apparent Km's for @entity3776 and @entity16749 were 6.86 X 10(-4) and 7.0 X 10(-5) M, respectively. In addition to the enzymes mentioned above, an @entity1110 +-linked malic enzyme was detected in the pure cultures but not in the vesicle clusters. In contrast, however, the vesicle clusters had activity of an @entity2125 -linked malic enzyme. The possibility that this enzyme resulted from contamination from plant mitochondria trapped in the vesicle clusters could not be discounted. None of the extracts showed activities of the Entner-Doudoroff enzymes or the @entity12742 metabolism enzymes @entity12742 dehydrogenase or gluconokinase. @entity4919 - versus @entity7970 -grown cultures of strain HFPArI3 showed similar activities of most enzymes except malic enzyme, which was higher in the cultures grown on the organic acid. @entity1568 -fixing cultures of strain HFPArI3 showed higher specific activities of @entity3776 and @entity16749 dehydrogenases and phosphofructokinase than @entity3265 -grown cultures.
