Alternative complement pathway of XXXX ( @entity4576 ): molecular characterization, mapping and expression analysis of factors Bf/C2 and Df.

@entity10363 @entity2348 @entity4576 @entity70837 @entity13195
The complement system is important in both innate and adaptive host defense against microbial infection in vertebrates. It contains three pathways: the classical, alternative, and lectin pathways. Complement component factors B and D are two crucial proteases in the alternative pathway. In this study, the genes of complement factors Bf/C2 and Df from @entity4576 , @entity4576 were identified and characterized. Two @entity70837 -related genes, Bf/ @entity10363 and Bf/C2B, and factor D gene Df were identified. Phylogenetic analysis suggested that Bf/ @entity10363 and Bf/C2B is likely orthologous to factor B and factor C2, respectively. Southern blot results suggested that these three genes are all single-copy genes in the catfish genome. The catfish Bf/ @entity10363 , Bf/C2B and Df genes were genetically mapped on linkage group 3, 20 and 29, respectively. Bf/ @entity10363 and Bf/C2B are highly expressed in liver and kidney, while Df is highly expressed in gill and spleen. After infection with @entity13195 , the expression of Bf/ @entity10363 , Bf/C2B and Df genes were found to be remarkably induced in the gill, liver, spleen and kidney at some sampling times, indicating that these three complement factors play a pivotal role in immune responses after the @entity2348 in catfish.
