Glial NF-kB inhibition alters neuropeptide expression after sciatic nerve injury in XXXX .

@entity32 @entity1931 @entity9327 @entity19 @entity17234 @entity1897 @entity158 @entity3804 @entity12819 @entity8638 @entity9
We utilized a transgenic @entity19 model where nuclear factor kappa B (NF-kB) is selectively inhibited in @entity9327 ( @entity9327 ) expressing cells. The transgene, @entity9327 -IkBa-dn, overexpresses a dominant negative form of the inhibitor of NF-kB (IkBa) under the control of the @entity9327 promoter. In the present work, we sought to understand the impact of glial NF-kB inhibition on the expression of @entity158 mediating sensory neuropeptides @entity17234 and @entity12819 ( @entity12819 ) in a model of @entity9 in @entity19 . @entity8638 ( @entity8638 ) of the left sciatic nerve was performed on wild type (WT) and @entity9327 -IkBa-dn @entity19 . RT-PCR and immunohistological staining were performed in sciatic nerve and/or L4-L5 DRG tissue for @entity17234 , @entity12819 and macrophage marker @entity3804 . @entity9327 -IkBa-dn @entity19 had less mechanical and thermal @entity1931 compared to WT @entity19 post- @entity8638 . After @entity8638 , we observed @entity17234 upregulation in DRG and sciatic nerve, which was less in @entity9327 -IkBa-dn @entity19 . @entity12819 gene expression in the DRG increased transiently on day 1 post- @entity8638 in WT but not in @entity9327 -IkBa-dn @entity19 , and no evidence of @entity12819 upregulation in sciatic nerve post- @entity8638 was found. After @entity8638 , upregulation of @entity3804 in sciatic nerve was less in @entity9327 -IkBa-dn @entity19 compared to WT @entity19 , indicative of less macrophage infiltration. Our results showed that glial NF-kB inhibition reduces @entity17234 and @entity12819 expression, which are neuropeptides that correlate with @entity158 behavior and @entity32 after @entity1897 .
