[Activation and XXXX of Bone Marrow Immunocytes by Using Various Cytokines]

@entity137 @entity1192 @entity379 @entity1583 @entity1199
The study aimed to explore the changes of the immunocyte quantities and @entity137 in bone marrow, and how many @entity1583 progenitor cells retained after the bone marrow cells were activated by different combinations of various cytokines. Bone marrow cells were divided into four groups and were cultured in vitro: (1) Control: no cytokines were added. (2) @entity1199 group: bone marrow cells were activated by @entity1199 . (3) CD3-AK group: bone marrow cells were activated by @entity1199 and CD3-McAb. (4) CIK group: @entity379 , @entity1192 , @entity1199 and CD3-McAb were added. CFU-GM assay and CD3 phenotype detection were performed before and after activating culture in all groups. The changes of cell quantities during culture and @entity137 of cultured cells were tested. CD3 positive cells markedly increased in both CD3-AK and CIK groups. The cell numbers and @entity137 of CD3-AK and CIK groups were higher than those of control or @entity1199 group obviously after culture (P < 0.05). CFU-GM were decreased in all groups after culture and there had no significant difference among four groups. The combination of @entity1199 and CD3-McAb not only stimulates the proliferation of marrow immunocytes and increases their @entity137 but retains enough @entity1583 progenitor cells as well. This combination of cytokines can be used to purge autologous bone marrow in vitro.
