Oleate, not ligands of the receptor for advanced glycation end-products, promotes proliferation of XXXX arterial smooth muscle cells.

@entity1 @entity2366 @entity14625 @entity3952 @entity6 @entity11461 @entity1674 @entity11396 @entity14822 @entity4483 @entity731 @entity1718 @entity8557 @entity65
AIMS/HYPOTHESIS: @entity6 accelerates @entity65 caused by @entity731 . Accordingly, @entity6 accelerates @entity731 progression and increases arterial smooth muscle cell proliferation. We hypothesized that @entity6 can exert growth-promoting effects on smooth muscle cells via increased advanced glycation end-products or by dyslipidaemia. METHODS: Primary @entity1 arterial smooth muscle cells were stimulated with advanced glycation end-products, other ligands of the receptor for advanced glycation end-products or @entity1718 common in @entity1674 . Cell proliferation was measured as DNA synthesis, cell cycle distribution and cell number. Effects of oleate on cellular phospholipids, @entity2366 , @entity1674 and @entity8557 were analyzed by thin-layer chromatography, and @entity3952 accumulation into @entity2366 was confirmed by gas chromatography. RESULTS: @entity1 arterial smooth muscle cells express the receptor for advanced glycation end-products, but its ligands @entity11461 -modified proteins, @entity14625 -modified proteins, @entity4483 polypeptide and amyloid-beta (1-40) peptide, exert no mitogenic action. Instead, oleate, one of the most common @entity1718 in @entity1674 , enhances platelet-derived growth factor-BB-mediated proliferation and oleate-containing @entity11396 formation in smooth muscle cells. This mitogenic effect of oleate depends on phospholipase D activity and is associated with an increased formation of oleate-enriched @entity11396 . CONCLUSION/INTERPRETATION: Oleate, not ligands of the receptor for advanced glycation end-products, acts as an enhancer of @entity1 smooth muscle cell proliferation. Thus, @entity14822 , rather than hyperglycaemia, could be a major factor promoting proliferation of smooth muscle cells in @entity731 .
