[Hematopoietic reconstitution in the allogeneic bone marrow chimeric XXXX ].

@entity19 @entity281 @entity2132 @entity7419
Hematopoietic reconstitution in the allogeneic chimera is an important issue because its failure causes fatal @entity281 in the host. Although @entity2132 ( @entity2132 ) often occurs and is a major obstacle for successful bone marrow transplantation (BMT), it does not always play an unfavorable role in the host. It has been suggested that @entity2132 may show some effects on the hematopoietic reconstitution. In order to elucidate mechanism of the influence of @entity2132 on the hematopoiesis, I have studied kinetics of the hematopoietic progenitor cells in the bone marrow chimeric @entity19 and the effects of spleen cells on it using in vitro @entity7419 assay. The number of @entity7419 in the bone marrow obtained from the allogeneic chimeras were reduced in the very early phase after BMT compared to that of syngeneic bone marrow chimeras. After then, the @entity7419 number in the allogeneic chimeras increased rapidly, especially in the chimeras showing a sign of severe @entity2132 . Addition of spleen cells from syngeneic chimeras to the bone marrow cell cultures showed no influence on the @entity7419 number observed. However, addition of spleen cells from allogeneic chimeras at 14 days after BMT to the bone marrow cells increased the @entity7419 number. By contrast, the chimeric spleen cells at 21 days after BMT decreased the number. These biphasic effects of spleen cells from allogeneic chimeras on the @entity7419 were considered to be mediated by some kinds of colony stimulating and inhibiting factors. Intraperitoneal injections of recombinant granulocyte-colony stimulating factors into bone marrow chimeras increased the number of granulocyte.(ABSTRACT TRUNCATED AT 250 WORDS)
