Comparative proteomics analysis of the root apoplasts of XXXX seedlings in response to @entity273 .

@entity274 @entity1735 @entity273 @entity1234 @entity579 @entity15
BACKGROUND: Plant apoplast is the prime site for signal perception and defense response, and of great importance in responding to environmental stresses. @entity273 ( @entity274 ) plays a pivotal role in determining the responsiveness of cells to stress. However, how the apoplast proteome changes under oxidative condition is largely unknown. In this study, we initiated a comparative proteomic analysis to explore @entity274 -responsive proteins in the apoplast of @entity579 seedling roots. METHODOLOGY/PRINCIPAL FINDINGS: 14-day-old @entity579 seedlings were treated with low concentrations (300 and 600   M) of @entity274 for 6 h and the levels of relative electrolyte leakage, @entity1234 and @entity274 were assayed in roots. The modified vacuum infiltration method was used to extract apoplast proteins of @entity579 seedling roots, and then two-dimensional electrophoresis gel analysis revealed 58 differentially expressed protein spots under low @entity274 conditions. Of these, 54 were successfully identified by PMF or @entity15 / @entity15 as matches to 35 different proteins including known and novel @entity274 -responsive proteins. Almost all of these identities (98%) were indeed apoplast proteins confirmed either by previous experiments or through publicly available prediction programs. These proteins identified are involved in a variety of processes, including redox homeostasis, cell wall modification, signal transduction, cell defense and @entity1735 metabolism, indicating a complex regulative network in the apoplast of seedling roots under @entity274 stress. CONCLUSIONS/SIGNIFICANCE: The present study is the first apoplast proteome investigation of plant seedlings in response to @entity274 and may be of paramount importance for the understanding of the plant network to environmental stresses. Based on the abundant changes in these proteins, together with their putative functions, we proposed a possible protein network that provides new insights into oxidative stress response in the @entity579 root apoplast and clues for the further functional research of target proteins associated with @entity274 response.
