In vitro XXXX and oxidative stress caused by amorphous @entity3440 nanoparticles.

@entity137 @entity1423 @entity19 @entity1234 @entity880 @entity460 @entity3440
Amorphous @entity3440 nanoparticles (SiNP) are being investigated for their potential use in various industrial and medical fields. Therefore, the assessment of their possible pathophysiological effect on circulating cells such as platelets is essential. We recently showed that intraperitoneal administration of SiNP causes proinflammatory and prothrombotic responses in vivo. However, little is known about the interaction of amorphous SiNP with platelets in vitro. Presently, we investigated the in vitro effects of SiNP (1, 5 and 25  g/ml) on @entity1423 , oxidative stress and intracellular @entity460 in @entity19 platelets. Incubation of platelets with SiNP caused a significant and dose-dependent @entity1423 . Similarly, the activity of @entity880 dehydrogenase (as a marker of cell membrane integrity) was significantly increased by SiNP. Total antioxidant activity and lipid platelets vulnerability to in vitro peroxidation (measured by @entity1234 production) were significantly increased after SiNP exposure. Additionally, SiNP exposure significantly increased the cytosolic @entity460 concentration. In conclusion, our in vitro data show that incubation of platelets with SiNP caused @entity1423 , oxidative stress and increased intracellular @entity460 . This finding provides evidence on the @entity137 of SiNP on platelet physiology.
