Comparison of the antibody response in adult @entity291 against different epitopes of XXXX lipopolysaccharide.

@entity6469 @entity558 @entity291 @entity3377
The comparison of serological responses in a sample of adult, vaccinated and held-infected @entity291 with @entity6469 is reported. Indirect enzyme immunoassav (EIA) titration curves and Western blotting tests for smooth-type lipopolysaccharide (S- @entity558 ), rough-type @entity558 (R- @entity558 ) and lipid A were performed. In the initial screening of sera, an overall prevalence of 20.5% was found, which corresponds to a country with a high incidence of @entity3377 . End-point EIA titres against @entity558 antigens from vaccinated and field-infected @entity291 were not significantly different. However, the absorbance values in the titration curves were significantly higher for S- @entity558 as compared with the other antigens. A high correlation coefficient (r = 0.933) was obtained when the titres to R- @entity558 versus lipid A were compared. Western blotting reactions of vaccinated and held-infected animals were indistinguishable. S- @entity558 , R- @entity558 and lipid A epitopes were recognized in a heterogeneous manner. In general, the number of @entity291 that reacted against @entity558 was higher in the field-infected group, with a stronger binding to S- @entity558 . Based on our observations, the vaccinated and field-infected @entity291 are capable of producing similar antibody responses to the Brucella main outer surface antigen, @entity558 . It should be emphasized that the humoral response of @entity291 to Brucella @entity558 contains significant amounts of antibodies to other antigenic moieties of this important surface molecule, which may contribute to the immunity to @entity3377 .
