An exogenous hydrogen sulphide donor, @entity8835 , inhibits the apoptosis signaling pathway to exert cardio-protective effects in a @entity35 XXXX model.

@entity3958 @entity548 @entity908 @entity23975 @entity4124 @entity12276 @entity8835 @entity880 @entity35 @entity3697 @entity3957
@entity3957 ( @entity3958 ) has been reported to be interwined in multiple systems, specifically in the cardiovascular system. However, the mechanisms underlying remain controversial. In the present study, we assessed the cardio-protective effects of @entity3958 in the @entity35 @entity548 model. @entity548 was induced in adult male @entity35 by drawing blood from the femoral artery to maintain the mean arterial pressure at 35-40 mmHg for 1.5 h. The @entity35 were assigned to four groups and the @entity3958 donor, @entity8835 (28  mol/kg, i.p.), was injected before the resuscitation in certain groups. After resuscitation the animals were observed and then killed to harvest the hearts. The morphological investigation and ultrastructural analyses were done and apoptotic cells were detected. The levels of relevant proteins were examined using Western blotting and immunohistochemical analyses. Resuscitated @entity548 induced @entity3697 and significantly increased the levels of serum myocardial enzymes, @entity908 kinase (CK) and @entity880 dehydrogenase (LDH) levels. Furthermore, it caused marked increase of apoptotic cells in heart tissue. Moreover, the expression of death receptor Fas and Fas-ligand, as well as the expression of apoptosis-relevant proteins active- @entity4124 and active- @entity12276 were markedly increased. Administration of @entity8835 significantly ameliorated @entity548 caused hemodynamic deterioration, decreased myocardial enzymes elevation, protected myocardial ultrastructure, and inhibited the expression of apoptosis-relevant proteins. It suggested that @entity3958 might exert its cardio-protective roles via both the extrinsic Fas/ @entity23975 / @entity12276 / @entity4124 pathway and the intrinsic mitochondria-involved pathways.
