Combined moist airtight storage and feed fermentation of XXXX by the @entity375 Wickerhamomyces anomalus and a lactic acid bacteria consortium.

@entity1707 @entity2247 @entity10751 @entity6376 @entity26044 @entity50472 @entity1336 @entity375
This study combined moist airtight storage of moist grain with @entity1707 feed fermentation. Starter cultures with the potential to facilitate both technologies were added to airtight stored moist crimped cereal grain, and the impact on storage microflora and the quality of feed fermentations generated from the grain was investigated. Four treatments were compared: three based on moist @entity2247 , either un-inoculated (M), inoculated with Wickerhamomyces anomalus (W), or inoculated with W. anomalus and @entity50472 starter culture, containing @entity6376 DSM 16243, @entity26044 DSM 12834 and @entity1336 DSM 12837 (WLAB); and one treatment based on dried @entity2247 (D). After 6 weeks of storage, four feed fermentations FM, FW, FWLAB, and @entity10751 , were initiated from M, W, WLAB, and D, respectively, by mixing the grain with water to a dry matter content of 30%. Each treatment was fermented in batch initially for 7 days and then kept in a continuous mode by adding new feed daily with 50% back-slop. During the 6 week storage period, the average water activity decreased in M, W and WLAB from 0.96 to 0.85, and cereal pH decreased from approximately 6.0 at harvest to 4.5. Feed fermentation conferred a further pH decrease to 3.8-4.1. In M, W and WLAB, molds and Enterobacteriaceae were mostly below detection limit, whereas both organism groups were detected in D. In fermented feed, Enterobacteriaceae were below detection limit in almost all conditions. Molds were detected in @entity10751 , for most of the fermentation time in FM and at some sampling points in FW and FWLAB. Starter organisms, especially W. anomalus and @entity1336 comprised a considerable proportion of the @entity375 and LAB populations, respectively, in both stored grain and fermented feed. However, autochthonous Pichia kudriavzevii and Kazachstania exigua partially dominated the @entity375 populations in stored grain and fermented feed, respectively.
