Influence of antigen distribution on the mediation of immunological XXXX .

@entity1 @entity644 @entity1310 @entity8 @entity11 @entity3626 @entity35
To determine if the site of immune reaction could influence the mediation and morphological expression of @entity8 in experimental anti-glomerular basement membrane (anti- @entity11 ) @entity1310 and @entity8 , we studied the events that followed the in situ reaction of rat antibody with antigen planted in either the @entity11 (especially the lamina rara interna) or in the subepithelial @entity3626 ( @entity3626 ). Non-nephritogenic amounts of noncomplement-fixing sheep anti- @entity11 or anti-tubular brushborder antibody were injected into separate groups of @entity35 to plant sheep IgG in the @entity11 and @entity3626 , respectively. Kidneys containing sheep IgG were then transplanted into naive recipients that were passively immunized with rat anti-sheep IgG. There was marked @entity644 after 2 days (antigen in @entity11 : 226 +/- 50.7; antigen in @entity3626 : 69 +/- 50.7 mg/24 hr) that was abrogated by prior depletion of complement in both groups (antigen in @entity11 : 10.2 +/- 1.7; antigen in @entity3626 : 14.3 +/- 8.7 mg/24 hr). When antigen was planted in @entity3626 , inflammatory-cell depletion with either anti-neutrophil (PMN) serum or lethal irradiation had no effect on @entity644 . In contrast, anti-PMN abolished @entity644 (12.0 +/- 5.6 mg/24 hr) and irradiation reduced it by 60% when antigen was in @entity11 . Glomeruli of kidneys with antigen in @entity11 were significantly larger and more hypercellular than those with antigen in @entity3626 after transplantation into immunized recipients. Endothelial cell injury and adherence of inflammatory cells to denuded @entity11 were prominent in the former (antigen in @entity11 ), while glomeruli with antigen in @entity3626 showed only subepithelial deposits, adjacent slit-diaphragm displacement, and epithelial cell foot-process effacement. Thus, the reaction of antigen and antibody in glomeruli produced complement-mediated injury which was cell-independent when complex formation occurred on the outer aspect of the @entity11 but was cell-dependent when the same reagents reacted more proximally to the circulation. We therefore conclude that antigen distribution can critically influence the mediation and morphologic expression of immune @entity8 and may, in part, account for variations in the clinical and histological manifestations of antibody-induced @entity8 in @entity1 .
