Application of bioluminescence imaging for in vivo monitoring of XXXX .

@entity1 @entity5 @entity5132 @entity281 @entity364 @entity54
Fungi can cause severe invasive @entity281 especially in the immunocompromised host. @entity1 populations at risk are increasing due to ongoing developments in @entity5 treatment and transplantation medicine. Only limited diagnostic tools and few antifungals are available, rendering a significant number of invasive @entity364 life threatening. To reduce mortality rates, a better understanding of the @entity281 processes is urgently required. Bioluminescence imaging (BLI) is a powerful tool for such purposes, since it allows visualisation of temporal and spatial progression of @entity281 in real time. BLI has been successfully used to monitor @entity281 caused by various microorganisms, in particular bacteria. However, first studies have also been performed on the fungi @entity5132 and @entity54 . Although BLI was, in principle, suitable to study the @entity281 process, some limitations remained. Here, different luciferase systems are introduced, and current approaches are summarised. Finally, suggestions for further improvements of @entity364 are provided.
