Role of microRNAs 221/222 on @entity6817 Induced XXXX Release in @entity1 Endothelial Cells.

@entity1 @entity3413 @entity9554 @entity6817 @entity2993 @entity3709 @entity842 @entity494 @entity10074 @entity1787 @entity165
BACKGROUND: @entity842 (NO) has been largely associated with cardiovascular protection through improvement of endothelial function. Recently, new evidence about modulation of NO release by microRNAs (miRs) has been reported, which could be involved with statin-dependent pleiotropic effects, including anti-inflammatory properties related to vascular endothelium function. OBJECTIVE: To evaluate the effects of @entity165 -lowering drugs including the inhibitors of @entity165 synthesis, @entity2993 and @entity3413 , and the inhibitor of @entity165 absorption @entity3709 on NO release, @entity9554 mRNA expression and miRs potentially involved in NO bioavailability. METHODS: @entity1 umbilical vein endothelial cells (HUVEC) were exposed to @entity2993 , @entity3413 or @entity3709 (0 to 5.0  M). Cells were submitted to total RNA extraction and relative quantification of @entity9554 mRNA and miRs -221, -222 and -1303 by qPCR. NO release was measured in supernatants by @entity1787 -chemiluminescence. RESULTS: Both @entity6817 increased NO levels and @entity9554 mRNA expression but no influence was observed for @entity3709 treatment. @entity2993 , @entity3413 and @entity3709 down-regulated the expression of @entity494 , whereas @entity10074 was reduced only after the @entity2993 treatment. The magnitude of the reduction of @entity494 and @entity10074 after treatment with @entity6817 correlated with the increment in @entity9554 mRNA levels. No influence was observed on the miR-1303 expression after treatments. CONCLUSION: NO release in endothelial cells is increased by @entity6817 but not by the inhibitor of @entity165 absorption, @entity3709 . Our results provide new evidence about the participation of regulatory miRs 221/222 on NO release induction mediated by @entity6817 . Although @entity3709 did not modulate NO levels, the down-regulation of @entity494 could involve potential effects on endothelial function.
