XXXX complicated @entity311 : Modulation and connectivity between exportome and variant surface antigen gene families.

@entity1 @entity8 @entity409 @entity177 @entity3964 @entity6197 @entity311 @entity4210
UNASSIGNED: In temperate and sub-tropical regions of Asia and Latin America, complicated @entity311 manifested as @entity409 or @entity8 is seen in all age groups. There has been a concerted focus on understanding the patho-physiological and molecular basis of complicated @entity311 in @entity1 , much less is known about it in adults. We report here, the analysis of data from a custom, cross strain microarray (Agilent Platform) using material from adult @entity1 samples, showing @entity409 or @entity177 . These are the most common manifestations seen in adults along with @entity6197 . The data has been analyzed with reference to variant surface antigens, encoded by the var, rifin and stevor gene families. The differential regulation profiles of key genes (comparison between @entity3964 complicated and uncomplicated isolates) have been observed. The exportome has been analyzed using similar parameters. Gene ontology term based functional enrichment of differentially regulated genes identified, up-regulated genes statistically enriched (P<0.05) to critical biological processes like generation of precursor metabolite and energy, chromosome organization and electron transport chain. Systems network based functional enrichment of overall differentially regulated genes yielded a similar result. We are reporting here, up-regulation of var group B and C genes whose proteins are predicted to interact with @entity4210 receptor in the host, the up-regulation of domain cassette 13 (DC13) containing var group A, as also the up-regulation of group A rifins and many of the stevors. This is contrary to most other reports from pediatric @entity1 , with @entity6197 where the up-regulation of mostly var A group genes have been seen. A protein-protein interaction based network has been created and analysis performed. This co-expression and text mining based network has shown overall connectivity between the variant surface antigens (VSA) and the exportome. The up-regulation of var group B and C genes encoding PfEMP1 with different domain architecture would be important for deciding strategies for disease prevention.
