Biocompatibility analysis of different biomaterials in XXXX bone marrow cell cultures.

@entity1 @entity702 @entity691 @entity692
A cell culture system for biocompatibility testing of hip implant materials is described. @entity1 bone marrow cells have been chosen because these cells are in direct contact with the biomaterial after implantation in situ. The sensitivity of this method is evaluated for materials which are already being used as implants in @entity1 and animal, e.g., @entity692 (HA) ceramic, pure @entity702 , and ultra-high-molecular-weight @entity691 (UHMWPE). As indicative parameters of biocompatibility primary cell adherence, cell number, cell proliferation, production of extracellular matrix, cell vitality, and cell differentiation are described. After 2 weeks in culture, obvious differences between the biomaterials with respect to the indicative parameters could be observed. Cell numbers were greatest on the HA specimens. In the case of @entity702 alloys, we observed a decreased number of cells. The production of extracellular matrix was high for the HA ceramics but reduced for @entity702 specimens. The polymers allowed only a few adherent cells and showed no signs of extracellular matrix production. The results can be correlated astonishingly well to animal experiments and clinical experiences. Therefore, we suggest that this cell culture system seems to be a useful tool for biocompatibility testing of bone implantation materials. It also helps reduce animal experiments. With the help of flow cytophotometry, we analyzed the influence of biomaterials on large numbers of cells with respect to differentiation. There were similar populations of T cells and monocytes on all specimens tested. Extended B-cell and granulocyte populations, however, were observed with @entity702 and UHMWPE. Most osteocalcin-containing cells adhered to the HA ceramics.
