Expression profiling of @entity19 @entity1204 microdissected from XXXX -fixed, @entity982 -embedded tissues.

@entity1204 @entity5 @entity75 @entity19 @entity55309 @entity982 @entity105 @entity1068
Expression-profiling studies have helped define genetic changes associated with @entity105 . Determining which alterations in gene expression are causally associated with @entity5 and which result from the general dysregulation in gene expression that is characteristic of @entity5 remains a problem. Transcriptional profiling of @entity75 (small @entity5 or precancers) holds promise for identifying biologically important changes in gene expression. There are, however, technical barriers to the study of small @entity5 . The total number of cells available for analysis is limiting. It is also often difficult to distinguish @entity5 cells from normal proliferating cells in frozen sections that are typically used as a source of RNA. Here we describe an @entity1068 fixation and @entity982 -embedding protocol that preserves tissue architecture and cellular morphology of the @entity19 endometrium, and allows for the recovery of high-quality RNA from microdissected cells. We performed GeneChip expression profiling using RNA from 800 to 4400 cells microdissected from @entity1068 -fixed, @entity982 -embedded uteri. Endometrial adenocarcinomas exhibited changes in the levels of a number of messages known to be abnormally expressed in @entity5 , and differential expression of additional transcripts not previously implicated in @entity105 . We confirmed increased @entity55309 expression in RNAs from @entity19 @entity1204 that were hybridized to GeneChips and validated overexpression of this transcript in additional @entity5 .
