Diagnostic value of immunoglobulin k light chain gene rearrangement analysis in XXXX .

@entity1 @entity706 @entity784 @entity1892 @entity2975 @entity1016 @entity7639
UNASSIGNED: Analysis of the immunoglobulin k light chain ( @entity1016 ) gene is an alternative method for B-cell clonality assessment in the diagnosis of mature B-cell proliferations in which the detection of clonal @entity7639 ( @entity7639 ) gene rearrangements fails. The aim of the present study was to evaluate the added value of standardized BIOMED-2 assay for the detection of clonal @entity1016 gene rearrangements in the diagnostic setting of suspected @entity1892 . With this purpose, 92 specimens from 80 @entity1 with the final diagnosis of @entity1892 (37 specimens), @entity784 (26 specimens) and reactive lymphoid proliferation (29 specimens) were analyzed for B-cell clonality. B-cell clonality analysis was performed using the BIOMED-2 @entity7639 and @entity1016 gene clonality assays. The determined sensitivity of the @entity1016 assay was 67.6%, while the determined sensitivity of the @entity7639 assay was 75.7%. The sensitivity of combined @entity7639 + @entity1016 assay was 81.1%. The determined specificity of the @entity1016 assay was 96.2% in the group of @entity784 and 96.6% in the group of reactive lesions. The determined specificity of the @entity7639 assay was 84.6% in the group of @entity706 and 86.2% in the group of reactive lesions. The comparison of GeneScan (GS) and heteroduplex pretreatment- @entity2975 gel electrophoresis (HD-PAGE) methods for the analysis of @entity1016 gene rearrangements showed a higher efficacy of GS analysis in a series of 27 @entity1892 analyzed by both methods. In the present study, we demonstrated that by applying the combined @entity7639 + @entity1016 clonality assay the overall detection rate of B-cell clonality was increased by 5.4%. Thus, we confirmed the added value of the standardized BIOMED-2 @entity1016 assay for assessment of B-cell clonality in suspected @entity1892 with inconclusive clinical and cyto/histological diagnosis.
