XXXX bryantii sp. nov., a rumen spirochete that interacts with cellulolytic bacteria.

@entity1501 @entity2765 @entity3363 @entity1885 @entity4588 @entity598 @entity3157 @entity9138 @entity515 @entity5993 @entity1735 @entity5189 @entity16642 @entity22780 @entity4571 @entity291 @entity413 @entity15898
A saccharolytic spirochete that associated and interacted with cellulolytic bacteria was isolated from @entity291 rumen fluid. Isolation was accomplished by means of a procedure involving serial dilution of a sample of rumen fluid into a cellulose-containing agar medium. Clear zones appeared within the medium as a result of cellulose hydrolysis by rumen bacteria. The saccharolytic spirochete and a cellulolytic bacterium later identified as a strain of Bacteroides succinogenes were isolated from the clear zones. The spirochete did not utilize cellulose, but grew in coculture with the cellulotytic bacterium in cellulose-containing media. When cocultured in these media the spirochete used, as fermentable substrates, soluble @entity3363 released from cellulose by the cellulolytic bacterium. In cellulose-containing agar medium the spirochete enhanced cellulose breakdown by the @entity15898 . Electron microscopy showed that the helical spirochete cells possessed an outer sheath, a protoplasmic cylinder, and two periplasmic fibrils. Under a @entity515 atmosphere, in a reduced medium containing inorganic salts, rumen fluid, @entity413 , and @entity5993 , the spirochete grew to a final density of 1.9 X 10(9) cells/ml. @entity4588 , @entity598 , and @entity5189 were products of the fermentation of @entity413 by growing cells. @entity515 ( @entity3157 ), branched @entity22780 , @entity1501 , @entity1885 , @entity4571 , @entity9138 , @entity2765 , and a @entity1735 were required for growth of the spirochete. The results of this study indicated that the rumen spirochete represents a new species of @entity16642 . It is proposed that the new species be named @entity16642 bryantii.
