Abnormal methylation status of XXXX and @entity28673 , and associations with clinical characteristics in @entity1358 .

@entity1 @entity28673 @entity5 @entity9736 @entity1358 @entity72657 @entity11508 @entity703
UNASSIGNED: The present study aimed to evaluate the use of the 27K methylation array to investigate abnormal methylation of two genes and their associations with clinical characteristics in @entity1358 ( @entity1358 ). Six differentially-methylated genes identified using the 27K methylation array were screened in the @entity1 @entity1358 786-0 cell line and normal kidney tissues by @entity9736 sequencing polymerase chain reaction (PCR). Differentially-methylated regions (DMRs) that were abnormally hypermethylated in the cell line were further validated in @entity703 and paired normal tissues by pyrosequencing. The correlations between DMRs and differences (methylation rate of @entity5 minus that of paired normal tissue) according to gender, age, @entity5 size, Fuhrman grade and disease stage were assessed. Gene expression prior to and following @entity11508 treatment was examined using reverse transcription quantitative PCR (RT-qPCR). Two DMRs located in the @entity72657 and @entity28673 genes were found to be hypermethylated in the 786-0 cells, but not in the normal kidney tissues. Pyrosequencing results showed that the average methylation rate of @entity72657 in the @entity5 tissues was significantly higher compared to that in the paired normal tissues (48.78 vs. 34.62%; P<0.001). The methylation rate of @entity28673 was also higher in the @entity5 tissues compared with the paired normal tissues (58.98 vs. 38.66%; P<0.001). In stage T1 @entity1358 , the methylation rate of the @entity5 tissue was positively correlated with the Fuhrman grade (P=0.02). The difference in methylation between the @entity5 and normal tissues was significantly higher in the group with high Fuhrman grade for the two genes. Furthermore, the linear correlation between methylation difference and @entity5 size was also confirmed (P=0.01). The RT-qPCR analysis demonstrated that @entity28673 and @entity72657 mRNA expression was significantly upregulated following @entity11508 treatment. The results identified two novel DMRs located in @entity28673 and @entity72657 that were hypermethylated in the @entity1358 tissue samples. The methylation profile in @entity1358 could potentially provide predictive information for clinical decisions.
