Evaluation of in vitro growth factor treatments on fibrochondrogenesis by synovial membrane cells from osteoarthritic and nonosteoarthritic joints of XXXX .

@entity19159 @entity571 @entity72686 @entity235 @entity72685 @entity26906 @entity58917 @entity720 @entity2720 @entity21180
OBJECTIVE: To determine the in vitro effects of selected growth factors on fibrochondrogenesis by synovial membrane cells from nonosteoarthritic (normal) and @entity2720 of @entity235 . ANIMALS: 5 @entity235 with secondary @entity571 of @entity26906 or stifle joints and 6 @entity235 with normal joints. PROCEDURES: Synovial membrane cells were harvested from normal and @entity2720 and cultured in monolayer with or without (control) basic fibroblast growth factor, transforming growth factor-b1, and insulin-like growth factor-1. In the cultured cells, fibrochondrogenesis was measured by use of a real-time reverse transcriptase PCR assay to determine relative expressions of collagen I, collagen II, and aggrecan genes and of 3 genes involved in embryonic chondrogenesis: @entity58917 ( @entity58917 ), frizzled-motif associated with bone development ( @entity72685 ), and @entity72686 ( @entity72686 ). Tissue collagen content was measured via a @entity720 assay, and @entity19159 content was measured via a @entity21180 assay. Cellularity was determined via a double-stranded DNA assay. Immunohistochemical analysis for collagens I and II was also performed. RESULTS: In vitro collagen synthesis was enhanced by growth factor stimulation. Although @entity2720 could undergo a fibrocartilage-like phenotypic shift, their production of collagenous extracellular matrix was less than that of normal-joint synoviocytes. Gene expressions of @entity58917 and @entity72686 were highest in the @entity2720 ; @entity72685 expression was highest in growth factor treated cells. CONCLUSIONS AND CLINICAL RELEVANCE: Autogenous synovium may be a viable cell source for meniscal tissue engineering. Gene expressions of @entity58917 and @entity72686 may be potential future targets for in vitro enhancement of chondrogenesis.
