Functionalization of XXXX surface with Chitosan via silanation: 3D CLSM imaging of cell biocompatibility behaviour.

@entity702 @entity1 @entity137
Introduction Biocompatibility ranks as one of the most important properties of dental materials. One of the criteria for biocompatibility is the absence of material @entity137 to cells, according to the ISO 7405 and 10993 recommendations. Among numerous available methods for @entity137 assessment; 3-dimensional Confocal Laser Scanning Microscopy (3D CLSM) imaging was chosen because it provides an accurate and sensitive index of living cell behavior in contact with chitosan coated tested implants. OBJECTIVES: The purpose of this study was to investigate the in vitro biocompatibility of functionalized @entity702 with chitosan via a silanation using sensitive and innovative 3D CLSM imaging as an investigation method for @entity137 assessment. Methods The biocompatibility of four samples (controls cells, TA6V, TA6V-TESBA and TA6V-TESBAChitosan) was compared in vitro after 24h of exposure. Confocal imaging was performed on cultured @entity1 gingival fibroblast ( @entity1 ) like cells using Live/Dead   staining. Image series were obtained with a FV10i confocal biological inverted system and analyzed with FV10-ASW 3.1 Software (Olympus France). Results Image analysis showed no @entity137 in the presence of the three tested substrates after 24 h of contact. A slight decrease of cell viability was found in contact with TA6V-TESBA with and without chitosan compared to negative control cells. Conclusion Our findings highlighted the use of 3D CLSM confocal imaging as a sensitive method to evaluate qualitatively and quantitatively the biocompatibility behavior of functionalized @entity702 with chitosan via a silanation. The biocompatibility of the new functionalized coating to @entity1 cells is as good as the reference in biomedical device implantation TA6V.
