Use of XXXX as an indicator of the origin of fecal water pollution.

@entity1 @entity14320 @entity72538 @entity57301 @entity33532 @entity27833 @entity291 @entity1237
A new, simple, and specific protocol to discriminate between @entity1 and animal fecal pollution is described. The procedure is based on the detection of certain @entity33532 species in the samples. Two 16S rRNA gene-targeted probes are described. One of these probes ( @entity27833 ) has as its target a region of the 16S rRNA gene of @entity57301 , a @entity33532 species of exclusively @entity1 origin. The other probe (BAN) is based on the sequence of a region of 16S rRNA gene for several @entity33532 species related with animal origins. The specificity of both probes was evaluated by using 24 @entity33532 species, and their threshold detection limit was established by DNA-DNA hybridization. DNA-DNA hybridization with the @entity27833 probe showed it to be specific for @entity57301 , whereas that with the BAN probe showed it to be specific for @entity14320 , @entity72538 , B. coryneforme, B. cuniculi, @entity33532 , B. magnum, B. minimum, and B. subtile. A simple and specific protocol was also developed for the detection of their target species in environmental samples (sewage and feces). DNA-DNA hybridization with the BAN probe was only positive for samples from @entity291 and @entity1237 . Thus, this probe is not suitable for the identification of any animal fecal pollution. Whereas all samples with @entity1 fecal pollution showed a positive DNA-DNA hybridization result with the @entity27833 probe, none of those with animal fecal pollution did. Therefore, this finding supports the potential use of this probe in detecting fecal pollution of @entity1 origin.
