Evaluation of bone regeneration potential of dental follicle stem cells for  treatment of XXXX .

@entity2122 @entity4351 @entity35
BACKGROUND AIMS: Stem cell-based tissue regeneration offers potential for treatment of @entity2122 . The dental follicle, a loose connective tissue surrounding the unerupted tooth, has been shown to contain progenitor/stem cells. Dental follicle stem cells (DFSCs) have strong osteogenesis capability, which makes them suitable for repairing skeletal defects. The objective of this study was to evaluate bone regeneration capability of DFSCs loaded into @entity4351 ( @entity4351 ) scaffold for treatment of @entity2122 . METHODS: DFSCs were isolated from the first mandibular molars of postnatal @entity35 and seeded into the @entity4351 scaffold. Cell attachment and cell viability on the scaffold were examined with the use of scanning electron microscopy and alamar blue reduction assay. For in  vivo transplantation, critical-size defects were created on the skulls of 5-month-old immunocompetent @entity35 , and the cell-scaffold constructs were transplanted into the defects. RESULTS: Skulls were collected at 4 and 8 weeks after transplantation, and bone regeneration in the defects was evaluated with the use of micro-computed tomography and histological analysis. Scanning electron microscopy and Alamar blue assay demonstrated attachment and proliferation of DFSCs in the @entity4351 scaffold. Bone regeneration was observed in the defects treated with DFSC transplantation but not in the controls without DFSC transplant. Transplanting DFSC- @entity4351 with or without osteogenic induction before transplantation achieved approximately 50% bone regeneration at 8 weeks. Formation of woven bone was observed in the DFSC- @entity4351 treatment group. Similar results were seen when osteogenic-induced DFSC- @entity4351 was transplanted to the critical-size defects. CONCLUSIONS: This study demonstrated that transplantation of DFSCs seeded into @entity4351 scaffolds can be used to repair @entity2122 .
