Anti- @entity5 properties of the XXXX /protein kinase G inhibitor @entity54739 in pancreatic adenocarcinoma.

@entity1 @entity6708 @entity1124 @entity6913 @entity39 @entity5 @entity19 @entity15983 @entity54739 @entity1995
UNASSIGNED: @entity1124 ( @entity1124 ) is one of the @entity5 in the world. Therefore, new therapeutic options are urgently needed to improve the survival of @entity1124 @entity1 . Protein kinase G (PKG) conducts the interlude of @entity6708 signaling which is important for healthy as well as for @entity5 cells. @entity54739 is a specific inhibitor of PKG, and it has been shown to possess an anti- @entity5 cytotoxic activity in vitro. The main aim of this work was to investigate anti- @entity5 effects of @entity54739 upon @entity1124 in vivo.Expression of PKG was assessed with real-time PCR analysis in the normal and @entity5 pancreatic cells. In vitro cell viability, proliferation, apoptosis, @entity39 , migration, and invasion of the @entity19 @entity1124 cell line Panc02 were assessed after @entity54739 treatment. In vivo anti- @entity5 effects of @entity54739 were investigated in the @entity19 Panc02 orthotopic model of @entity1124 . Western blot analysis was used to determine the phosphorylation state of the proteins of interest.Functional PKGI is preferentially expressed in @entity1124 cells. @entity54739 was capable to reduce viability, proliferation, and migration of @entity19 @entity1124 cells in vitro. At the same time, @entity54739 treatment did not change the viability of normal epithelial cells of @entity19 liver. In vivo, @entity54739 treatment reduced the @entity5 volume and metastases in @entity1124 -bearing @entity19 , but it was ineffective to prolong the survival of the @entity5 -bearing animals. In addition, @entity54739 treatment decreased phosphorylation of @entity6913 , @entity1995 , and @entity15983 in @entity19 @entity1124 .Inhibition of PKG could be a potential therapeutic strategy for @entity1124 treatment which should be carefully validated in future pre-clinical studies.
