Nonspecific cytotoxic cells in fish ( XXXX ). V. Metabolic requirements of lysis.

@entity1 @entity9310 @entity6193 @entity49030 @entity137 @entity706 @entity4527 @entity3213 @entity4576 @entity2389 @entity3812 @entity141 @entity4627 @entity1098 @entity5713
The mechanisms of lysis of nonspecific cytotoxic cells (NCC) from the @entity4576 ( @entity4576 ) were studied by determining the effects of various inhibitors of cellular metabolism on cytolysis of NC-37 @entity1 @entity706 target cells. Inhibition of NCC-mediated lysis by @entity9310 ( @entity3213 ) and @entity4627 ( @entity4627 ) indicated a requirement for cellular energy metabolism. @entity2389 , an inhibitor of microfilaments, and @entity6193 , an inhibitor of cellular secretion, both prevented lysis by NCC. Three microtubule inhibitors, @entity5713 , @entity3812 , and @entity49030 , all inhibited target cell lysis. Two divalent cation chelators, @entity1098 and @entity4527 , blocked NCC activity. Elimination of both @entity141 and Mg2+ by @entity1098 prevented target cell binding and killing. Selective removal of @entity141 by @entity4527 prevented killing but did not block target cell binding. These results indicated that nonspecific @entity137 in fish is an active process which requires cell movement and an intact secretory apparatus. The mechanisms of cytolysis by NCC were found (except for the requirement of microtubules) to be analogous to those of @entity1 NK cells. Combined with morphological studies, these data strongly suggest that a phylogenetic relationship exists between these effector cells.
