[Anti-mesangial cell autoantibodies and their target antigens in XXXX ].

@entity1 @entity8 @entity1988 @entity5417 @entity811
OBJECTIVE: Anti-mesangial cell autoantibodies (anti-MC) have been reported in @entity1 with @entity8 and @entity5417 . The aim of the current study was to identify anti-MC autoantibodies in sera from @entity1 with @entity1988 ( @entity1988 ). METHODS: 96 serum samples were collected from 96 @entity1 with renal biopsy proven @entity1988 . Anti-MC autoantibodies were examined by cellular ELISA and Western blot analysis. In vitro cultured @entity1 mesangial cells were used as cell substrate in ELISA and the their soluble proteins in non-reducing conditions were used as antigens in Western-blot analysis. RESULTS: 37.5% and 97.9% of the serum samples from @entity1 with @entity1988 had anti-MC autoantibodies in cellular ELISA and Western blot analysis respectively, and 12 protein bands could be blotted by the @entity1988 sera in Western blot analysis. The percentage of anti-63 000 autoantibody positive samples was significant higher in female @entity1 than that in the male (59.8% vs 21.4%, P < 0.01). The prevalence of anti-74 000, 46 000, and 36 000 autoantibodies were significantly higher in @entity1 with @entity811 than that in @entity1 without @entity811 (36.8% vs 5.0%, P < 0.01; 34.2% vs 10.0%, P < 0.05; 31.6% vs 5.0%, P < 0.05; respectively). The prevalence of anti-63 000 autoantibodies was also significantly higher in @entity1 with ANA than that in @entity1 without (67.6% vs 16.7%, P < 0.001). The prevalence of anti-18 000 autoantibodies was significantly higher in patients with anti-dsDNA antibodies than that in @entity1 without (61.5% vs 34.4%, P < 0.05). CONCLUSIONS: There are heterogeneous anti-MC autoantibodies in sera from @entity1 with @entity1988 . Anti-MC autoantibodies may have a pathogenic role in @entity1988 and some anti-MC autoantibodies may be associated with different clinical manifestations.
