Function of exudate neutrophils from skin in XXXX .

@entity1 @entity375 @entity1569 @entity5037 @entity4514
@entity1 neutrophils harvested from skin chambers containing autologous serum in psoriatic @entity1 were compared with peripheral blood neutrophils by examining migration, phagocytosis, and oxidative activation. Random migration was reduced in exudate cells, whereas the chemotactic response to yeast-activated serum was evident not only in blood neutrophils, but also in exudate cells. The metabolic activation of exudate neutrophils, when stimulated with @entity5037 and measured as chemiluminescence, was enhanced by 50-400% compared to blood neutrophils. The chemiluminescence response to @entity4514 was on the other hand reduced to 35%. In the phagocytic assay, using C3bi- and IgG-opsonized @entity375 particles, exudate neutrophils from non-psoriatic healthy controls showed enhanced uptake of C3bi-coated @entity375 compared to blood neutrophils. In psoriatics, the blood neutrophils showed enhanced C3bi-mediated phagocytosis compared to non-psoriatic control cells. No further increase in C3bi-mediated phagocytosis was then seen in exudate cells from these patients. IgG-mediated phagocytosis was in contrast to C3bi similar between blood and exudate neutrophils in psoriatics and non-psoriatic controls. These experiments show that during exudation priming of different receptor-mediated processes can occur. However, no significant difference was observed between different functional capacities in exudate neutrophils from @entity1569 @entity1 and non-psoriatic controls.
