Expression, stability, and replacement of glucan-remodeling enzymes during developmental transitions in XXXX .

@entity72639 @entity72640 @entity72641 @entity21755 @entity4495 @entity375 @entity62623 @entity72638 @entity72637
Sporulation is a developmental variation of the @entity375 life cycle whereby four spores are produced within a diploid cell, with proliferation resuming after germination. The GAS family of @entity4495 -anchored glucan-remodeling enzymes exemplifies functional interplay between paralogous genes during the @entity375 life cycle. @entity62623 and @entity72637 are expressed in vegetative cells and repressed during sporulation while @entity72638 and @entity72639 exhibit a reciprocal pattern. @entity72640 is weakly expressed in all the conditions and encodes an inactive protein. Although @entity62623 functions in cell wall formation, we show that it persists during sporulation but is relocalized from the plasma membrane to the epiplasm in a process requiring @entity21755 -mediated endocytosis and the @entity72641 protein kinase of the p21-activated kinase family. Some @entity62623 is also newly synthesized and localized to the spore membrane, but this fraction is dispensable for spore formation. By way of contrast, the @entity72638 - @entity72639 proteins, which are essential for spore wall assembly, are rapidly degraded after spore formation. On germination, @entity62623 is actively synthesized and concentrated in the growing part of the spore, which is essential for its elongation. Thus @entity62623 is the primary glucan-remodeling enzyme required in vegetative growth and during reentry into the proliferative state. The dynamic interplay among Gas proteins is crucial to couple glucan remodeling with morphogenesis in developmental transitions.
