Correlations between hematopoietic progenitor cell counts as measured by Sysmex and XXXX + cell harvest yields following mobilization with different regimens.

@entity1 @entity1261 @entity706 @entity5 @entity1582 @entity1583 @entity2280
PURPOSE: The Sysmex SE-9000 cell counter provides an estimate of immature cells referred to as @entity1583 ( @entity1583 ). @entity1583 counts should correlate with @entity2280 + counts in mobilized peripheral blood and apheresis to allow optimization of apheresis timing. METHODS: We correlated the @entity1583 counts as measured in the immature information channel with @entity2280 + cell levels as determined by FACS (HPCA-2 antibody, Becton Dickinson) from mobilized peripheral blood in 40 samples (27 @entity1 and three healthy donors) and in aphereses ( n=113, 41 @entity1 and 20 healthy donors). RESULTS: In mobilized blood, @entity1583 counts were correlated with @entity2280 + cells ( r=0.78, P<0.0001, n=40). The @entity1583 counts were about 1.5-fold higher than @entity2280 + cell counts with a median (range) of 84 (1-747)/microl and 57 (1-370)/microl, respectively. In @entity2280 + selected cell preparations ( n=8), @entity1583 counts were about fourfold lower than @entity2280 + cell counts with a median (range) of 179 (67-693)/microl and 760 (191-4309)/microl, respectively. In apheresis preparations, linear regression analyses were performed for the group of stem cell donors ( n=44), the group of @entity706 @entity1 ( n=23), the multiple @entity1582 group ( n=21), and the group of solid @entity5 ( n=25). Interestingly, no correlation between @entity1583 counts and @entity2280 + cell counts was found in the @entity1261 -mobilized healthy donor group ( r=0.23, P=0.13). Pairing of @entity1583 counts and @entity2280 + counts was effective in the group of @entity1 receiving chemotherapy + @entity1261 for stem cell mobilization: @entity706 group ( r=0.67, P=0.0005), multiple @entity1582 group ( r=0.56, P=0.008), and the group of solid @entity5 ( r=0.52, P=0.007). CONCLUSIONS: Lymphoma and multiple @entity1582 @entity1 who were moderately pretreated and mobilized with chemotherapy and @entity1261 showed the best results in correlation analyses even at low @entity1583 counts. Therefore, @entity1583 measurement can be used for timing of apheresis in these @entity1 .
