Dynein/Dynactin-mediated transport of kinetochore components off kinetochores and onto spindle poles induced by XXXX .

@entity72884 @entity23506 @entity2605 @entity2536 @entity38451 @entity2604 @entity72885 @entity72883 @entity18374 @entity20400 @entity19136
The mitotic checkpoint functions to ensure accurate chromosome segregation by regulating the progression from metaphase to anaphase. Once the checkpoint has been satisfied, it is inactivated in order to allow the cell to proceed into anaphase and complete the cell cycle. The minus end-directed microtubule motor dynein/dynactin has been implicated in the silencing of the mitotic checkpoint by "stripping" checkpoint proteins off kinetochores. A recent study suggested that @entity2604 ( @entity2605 ) stimulates dynein/dynactin-mediated transport of its cargo including @entity18374 ( @entity18374 ). We analyzed the effects of @entity2605 on dynein/dynactin dependent transport of the RZZ ( @entity18374 , Roughdeal, @entity72883 ) complex as well as other kinetochore components from kinetochores to spindle poles. Through this approach we have catalogued several kinetochore and centromere components as dynein/dynactin cargo. These include @entity18374 , @entity72883 , @entity23506 , @entity72884 , @entity20400 , @entity2536 , @entity38451 , @entity19136 , cyclin-B and @entity72885 . Furthermore, we found that treatment with @entity2605 induced a robust accumulation and complete stabilization of @entity18374 at spindle poles. This finding suggests that @entity2605 may not induce dynein/dynactin transport but rather interfere with cargo release. Lastly, we determined that @entity2605 induced accumulation of checkpoint proteins at the poles requires dynein/dynactin-mediated transport, @entity18374 kinetochore localization and kinetochore-microtubule attachments but not tension or Aurora B kinase activity.
