Cloning and Functional Analysis of @entity2655 Family Genes from XXXX .

@entity5132 @entity375 @entity2655 @entity5999
With a 0.5 kb probe of CX2, distribution of CX2 tandem repeats was studied in different @entity5132 strains. Results suggest that all the @entity5132 strains tested contained the tandem repeat. In order to verify if the expression of CX2 was hyphal specific, its expression was analyzed under various inductive and non-inductive conditions. With CX2 0.5 kb probe, Northern hybridization analysis confirmed that it was specifically in hyphae. The result of chromosomal localizationtion and genomic Southern blot analysis suggested that there were other genes containing the tandem repeat besides of ALS1. A @entity5132 s genomic DNA library was screened with the CX2 0.5 kb probe and several positive recombinant lambda phages were obtained. After endonuclease identification, subcloning, and sequence analysis, several @entity2655 family genes were cloned. No.1 @entity5999 DNA contained ALS4, No.4 @entity5999 DNA contained ALS1, No.6 @entity5999 DNA contained ALS3. To study the role of @entity2655 family genes in @entity375 -hyphal transition, als1/ALS1 mutant was constructed by homologous recombination. The ability to form hyphae was tested in different inductive culturing conditions. Defective hyphal growth were observed in some solid media.
