Preparation, characterisation and antibacterial activity of a XXXX -loaded solid lipid nanoparticle suspension.

@entity137 @entity19 @entity21562 @entity794 @entity281 @entity526
A @entity21562 -loaded solid lipid nanoparticle (FFC-SLN) suspension was prepared by hot homogenisation and ultrasonic technique. The suspension was characterised for its release profile, stability, @entity137 , and the physicochemical properties of the nanoparticles. Antibacterial activity of the suspension was evaluated in vitro and in vivo. The results showed that the mean diameter, polydispersity index and zeta potential of the nanoparticles were 253    3 nm, 0.409    0.022 and 47.5    0.21 mV, respectively. In vitro release profile showed the FFC-SLN suspension had sustained release effect. The minimum inhibition concentration values of the FFC-SLN suspension were 6 and 3   g/mL against @entity794 and @entity526 respectively, compared with 3.5 and 2   g/mL of native @entity21562 . The suspension was relatively stable at 4  C and less stable at room temperature during 9 months storage. Although the nanoparticle carriers exhibited @entity137 in cell cultures, the LD50 of the lyophilised dry power of the suspension was higher than 5 g/kg body weight. Mortality protection against @entity526 lethal @entity281 in @entity19 showed that the nanoparticle suspension had much better efficacy (6/10) than native drug (1/10). These results indicate that FFC-SLN suspension could be a promising formulation in veterinary medicine.
