Lentivirus mediated silencing of ubiquitin specific peptidase 39 inhibits cell proliferation of @entity1 XXXX cells in vitro.

@entity1 @entity72155 @entity0 @entity4404 @entity33986 @entity98 @entity157
BACKGROUND: Ubiquitin Specific Peptidase 39 ( @entity33986 ) is a 65  kDa @entity72155 involved in RNA splicing. Previous studies showed that @entity33986 is related with tumorigenesis of @entity1 @entity0 cells. RESULTS: In the present study, we investigated the functions of @entity33986 in @entity1 @entity157 ( @entity157 ) cell line SMMC-7721. We knocked down the expression of @entity33986 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after @entity33986 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by @entity98 assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after knock-down of @entity33986 . Furthermore, suppression of @entity33986 arrested cell cycle progression at G2/M phase in SMMC-7721cells. In addition, @entity4404 showed that downregulation of @entity33986 significantly increased the population of apoptotic cells. CONCLUSIONS: All our results suggest that @entity33986 is important for @entity157 cell proliferation and is a potential target for molecular therapy of @entity157 .
