Primary structure of the trpC gene from XXXX .

@entity2036 @entity535 @entity377 @entity14940 @entity1980
We have determined the structure and complete @entity377 sequence of the trifunctional trpC gene from the Ascomycetous fungus @entity14940 . Results from RNA gel blot analyses showed that this gene encodes two size classes of polyribosomal, poly (A)+RNAs with approximate lengths of 2,400 and 2,600 @entity2036 . S1 nuclease protection studies demonstrated that the distribution into the two size classes is due to selection of alternative sites for polyadenylation. The transcription units contain a single open translation reading frame of 2,304 @entity2036 . The sequence of this reading frame is approximately 40% divergent from the sequence of the functionally analogous trp-1 gene from @entity1980 (Schechtman, M.G. and Yanofsky, C., J. Mol. Appl. Gen. 2:83-99). The predicted amino acid sequence of the A. nidulans trpC polypeptide is also 40% divergent from the predicted @entity535 sequence of the @entity1980 trp-1 polypeptide. The A. nidulans gene has considerably less bias in codon selection than observed for the @entity1980 gene. Discrete regions of DNA homology were also found in similar positions in the 5' and 3' flanking sequences of the Aspergillus and @entity1980 genes. Similar regions of homology were not observed in other Aspergillus or @entity1980 genes that have been sequenced. Thus, if these evolutionarily conserved sequences act as signals for transcription initiation or polyadenylation, or are involved in gene regulation, their functions are restricted to a subset of protein coding genes in these two closely related fungi.
