Do major histocompatibility complex tag single nucleotide polymorphisms accurately identify XXXX in the Turkish population?

@entity1 @entity73249 @entity73251 @entity1530 @entity1691 @entity73250 @entity73248
OBJECTIVE: To evaluate the performance of human @entity1530 tag single nucleotide polymorphisms (SNPs) by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). METHODS: We genotyped three SNPs ( @entity73248 , @entity73249 and @entity73250 ). The primers were designed using Primer3 algorithm via primer-BLAST interface. PCR products were digested by using NlaIII, BmrI and TaqaI enzymes. Quality control was performed by DNA sequence analysis. RESULTS: In total, 207 @entity1 with @entity1691 and 32 healthy controls were included in the study. The sensitivity and specificity of SNPs @entity73248 and @entity73250 in identifying @entity1530 were identical and adequate at 0.946 and 1.000, respectively. On the other hand, the sensitivity and specificity for @entity73249 was 0.878 and 0.934, respectively. The presence of another SNP ( @entity73251 ) in close proximity to @entity73248 complicated the analysis for RFLP and required that we sequence all the T allele carrying samples. CONCLUSION: The SNPs @entity73248 and @entity73250 may have a place in the identification of @entity1530 in the Turkish population; however, methods other than PCR-RFLP should be considered.
