Urokinase type plasminogen activator receptor is involved in insulin-like growth factor-induced XXXX of @entity980 cells in vitro.

@entity19518 @entity12067 @entity2289 @entity10431 @entity5 @entity4222 @entity8993 @entity442 @entity5100 @entity534 @entity7788 @entity3 @entity31158 @entity980 @entity4495 @entity54181 @entity2290
@entity2290 ( @entity2290 ) binds to its receptor, @entity10431 , on the surface of @entity5 cells, leading to the formation of plasmin. @entity980 ( @entity980 ) cell lines secrete high levels of @entity5100 ), suggesting autocrine IGFs play a major role in the unregulated growth and @entity3 of @entity980 . In vitro, @entity5100 and @entity442 increased @entity4222 of RD cells to 124+/-9% (P<0.01) and 131+/-8% (P<0.05) of control, respectively. @entity5100 -induced @entity4222 was abolished by @entity19518 ( @entity19518 ) (P<0.01), a relatively specific inhibitor of @entity5100 , and by @entity2289 ( @entity2289 ) (P<0.05). Aprotinin, a plasmin inhibitor, and @entity31158 , which inhibits the synthesis of @entity4495 (GPI), thereby preventing anchorage of GPI-linked proteins such as @entity10431 to the cell membrane, also decreased @entity5100 - (P<0.05 for both) but not @entity442 -induced @entity4222 . [ @entity534 54, @entity534 55] @entity5100 and [Leu27] @entity5100 , which preferentially bind to the @entity442 and @entity5100 / @entity7788 receptors ( @entity5100 / @entity54181 ), respectively, both induced RD cell @entity4222 to 146+/-8% (P<0.01) and 120+/-7% (P<0.05) of control, respectively. An anti- @entity10431 anti-serum reduced @entity5100 - and @entity442 -induced @entity4222 (P<0.05 for both). An anti-low density @entity8993 ( @entity8993 ) anti-serum reduced @entity442 -induced @entity4222 (P<0.05). @entity12067 both increased specific 125I-single chain @entity2290 (scuPA) binding to RD cells in a dose-dependent manner (P<0.01). These results suggest involvement of the PA/plasmin system in IGF-induced @entity4222 and indicate important roles these systems may have in @entity980 @entity3 .
