A novel transactivating factor that regulates XXXX -dependent gene expression.

@entity1 @entity7263 @entity19 @entity60 @entity38919 @entity238
We have previously identified a novel interferon (IFN)-stimulated cis-acting enhancer element, gamma-IFN-activated transcriptional element (GATE). GATE differs from the known IFN-stimulated elements in its primary sequence. Preliminary analysis has indicated that the GATE-dependent transcriptional response requires the binding of novel transacting factors. A cDNA expression library derived from an @entity60 -stimulated @entity19 macrophage cell line was screened with a @entity7263 -labeled GATE probe to identify the potential GATE-binding factors. A cDNA coding for a novel transcription-activating factor was identified. Based on its discovery, we named it as @entity38919 ( @entity38919 ). @entity38919 homologs are present in @entity19 , @entity1 , monkey, and @entity238 . It activates transcription from reporter genes carrying GATE. It possesses a strong transactivating activity but has a weak DNA binding property. @entity38919 is expressed in most tissues with relatively higher steady-state levels in heart, liver, kidney, and brain. Its expression is induced by @entity60 treatment. @entity38919 is present in both cytosolic and nuclear compartments. These studies thus identify a novel transactivating factor in IFN signaling pathways.
