Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in XXXX .

@entity1 @entity742 @entity5 @entity19 @entity2822 @entity1655 @entity465 @entity2823 @entity5231 @entity2670 @entity1611
BACKGROUND: Aggressive anti- @entity5 treatments can result in @entity2670 . Ovarian cryopreservation has been developed to preserve the fertility of young @entity1 , but early graft revascularisation still requires improvement. METHODS: Frozen/thawed @entity742 ovarian cortical biopsies were embedded in collagen matrix with or without isoform 165 of vascular endothelial growth factor ( @entity1655 ) and transplanted into ovaries of @entity465 @entity19 . Ovaries were chosen as transplantation sites to more closely resemble clinical conditions in which orthotopic transplantation has previously allowed several spontaneous pregnancies. RESULTS: We found that @entity1655 significantly increased the number of Dextran- @entity2823 positive functional vessels 3  days after grafting. Dextran- @entity2822 ( @entity2823 ) positive vessels were detectable in 53% and 29% of the @entity19 in the @entity1611 -treated and control groups, respectively. Among these positive fragments, 50% in the treated group displayed mature smooth-muscle-actin-alpha (alpha-SMA) positive functional vessels compared with 0% in the control group. @entity5231 positive @entity19 blood vessels were observed in 40% of the @entity1655 transplants compared with 21% of the controls. After 3  weeks, the density of @entity19 vessels was significantly higher in the @entity1655 group. CONCLUSION: The encapsulation of ovarian tissue in collagen matrix in the presence of @entity1655 before grafting has a positive effect on functional blood vessel recruitment. It can be considered as a useful technique to be improved and further developed before @entity1 clinical applications in female @entity5 @entity1 in the context of fertility preservation.
