Adverse prognostic effect of methylation in @entity14 is reversed by XXXX .

@entity1 @entity6756 @entity124 @entity986 @entity5 @entity17012 @entity17011 @entity9736 @entity14
PURPOSE: DNA methylation is an important biologic event in @entity14 and in some cases is associated with the development of @entity124 ( @entity124 ). In this study, we sought to determine the prognostic significance of DNA methylation, both in univariate analysis and in concert with other clinicopathologic factors known to influence outcome. @entity1 AND METHODS: Fresh tissue (625 @entity5 ) was obtained from 605 individuals (age range, 29 to 99 years) undergoing curative surgery for @entity14 at one institution during a period of 8 years. Clinicopathologic details were recorded for all @entity5 , including stage, grade, type, vascular space invasion, and clinical follow-up to 5 years. Microsatellite status was assessed using standard markers. Methylation of @entity986 and @entity6756 promoters was determined by methylation-specific polymerase chain reaction (PCR), whereas methylation at methylated-in- @entity5 loci ( @entity17011 , @entity17012 , MINT12, and MINT31 loci were assessed by @entity9736 -PCR. RESULTS: @entity1 with microsatellite unstable @entity5 (12%) had better disease-specific survival than those with microsatellite stable (MSS) @entity5 (univariate analysis: hazard ratio [HR], 0.53; 95% CI, 0.27 to 1.0). Overall survival of individuals with @entity5 was influenced by three independently significant factors: @entity5 stage (HR, 7.3; 95% CI, 5.1 to 10.4), heavy @entity5 methylation (HR, 2.1; 95% CI, 1.1 to 4.0), and vascular space invasion (HR, 1.9; 95% CI, 1.3 to 2.9). In @entity5 , methylation at any single site was not independently predictive of survival. Neither methylation nor microsatellite status predicted a favorable response to chemotherapy. CONCLUSION: DNA methylation is associated with a worse outcome in @entity14 , but this adverse prognostic influence is lost in those @entity5 showing @entity124 . The mechanisms of these events warrant additional investigation.
