Evidence that cold preservation-induced @entity130 in liver allografts is not mediated by @entity26 -free radicals or cell swelling in the XXXX .

@entity2656 @entity1792 @entity1219 @entity26 @entity3282 @entity969 @entity1877 @entity2421 @entity1017 @entity9275 @entity35 @entity208 @entity130
The critical injury of cold preservation is to the hepatic microcirculation. @entity26 free @entity130 and cell swelling have been proposed to be causes of allograft failure, and new solutions such as Marshall's isotonic @entity1792 and University of Wisconsin (UW) solutions were designed to prevent cell swelling and free @entity130 . Experiments were done to determine whether Marshall's solution and UW solution protect the microcirculation, and whether they do so by preventing cell swelling or free radical-induced injury. To determine if the new solutions reduce sinusoidal lining cell injury, @entity35 livers were examined after preservation at 4 degrees C in @entity1219 0.9% and @entity3282 2 mM for 4 hr and 8 hr, in Collins' solution for 8 hr, and in both UW and isotonic @entity1792 solutions for 8 hr and 16 hr. Next, the role of cell swelling in @entity130 was studied by storing livers in @entity2421 solutions that accelerate liver @entity1017 , and in a @entity1877 -based preservation solution. Finally, to evaluate the role of active @entity26 species, SOD, catalase, and @entity208 were added to preservation solutions. The effect of @entity208 alone was also studied. In a related study, @entity969 was substituted for the free radical scavenger, @entity2656 , in isotonic @entity1792 solution. All livers were studied by light microscopy after perfusion-fixation. Storage in UW and isotonic @entity1792 solutions resulted in clear improvement in the morphology of the sinusoidal lining. Increasing the rate of liver @entity1017 by the use of @entity2421 solutions did not accelerate the endothelial injury. @entity1877 -based solution prevented @entity1017 during preservation, but unlike UW or @entity9275 solutions it did not retard the @entity130 . After preservation in the presence of SOD and catalase, or @entity208 , no improvement in the defined morphological features of the endothelial injury was noted when compared with respective controls; nor was the benefit of isotonic @entity1792 solution lessened by the removal of the free radical scavenger @entity2656 . We conclude that @entity130 produced by @entity130 is due neither to free radical-mediated injury nor to cell swelling. As both UW and isotonic @entity1792 solutions provide significant protection to the microcirculation, they must do so by a yet-undetermined mechanism.
