Immunohistochemical evaluation of sentinel lymph nodes in XXXX @entity1 .

@entity1 @entity72412 @entity0 @entity1711 @entity1600 @entity189 @entity1325 @entity1710 @entity3
@entity1600 sampling has become an alternative to axillary lymph node dissection to provide prognostic and treatment information in @entity0 @entity1 . The role of immunohistochemistry has yet to be established. A total of 241 sentinel lymph nodes (in 270 slides) from 91 @entity1 with @entity189 (73 ductal, 9 lobular, 8 mixed lobular/ductal, 1 NOS) were studied for presence of macrometastases (> 0.2 cm), identified in @entity1710 and @entity1711 sections, and occult metastases (micrometastases [< or = 0.2 cm], clusters of cells, @entity189 cells), identified only by immunohistochemistry. Intraoperative touch preparations, frozen sections, seven @entity1710 and @entity1711 levels (L1-L7), and two @entity1325 -3 cytokeratin immunohistochemistries (L1, L4-5) of the entire bisected or trisected sentinel lymph node were examined. Thirty-one (34%) @entity1 had 50 positive sentinel lymph nodes. Twenty-six (33%) sentinel lymph nodes had @entity189 (11 macrometastases, 11 micrometastases, 3 clusters of cells, 1 @entity189 cells) by touch preparations, frozen sections, and one @entity1710 and @entity1711 (L1). Thirty-eight (43%) were positive by @entity1325 -3 immunohistochemistry (L1) (11 macrometastases, 8 micrometastases, 13 clusters of cells, 6 @entity189 cells), significantly more than by touch preparations, frozen sections, @entity1710 and @entity1711 L1, or @entity1710 and @entity1711 @entity72412 . Cytokeratin immunostain on L4-5 demonstrated 31 (34%) positive sentinel lymph nodes, a similar frequency to cytokeratin immunostain on L1. Size of sentinel lymph node @entity3 did not correlate with size, histologic grade, or type of primary @entity0 . @entity1325 -3 (L1) immunohistochemistry is highly sensitive in delineating sentinel lymph node @entity3 , especially clusters of cells and @entity189 cells. The prognostic significance of clusters of cells and @entity189 cells and the value of @entity1325 -3 immunohistochemistry on frozen sections need to be determined.
