@entity14660 and XXXX are required for @entity8 .

@entity8273 @entity8 @entity14660 @entity19 @entity14661
@entity14660 ( @entity14660 ) and @entity14661 ( @entity14661 ) are the major hyaluronidases acting synergistically to degrade @entity8273 ( @entity8273 ). In the kidney, @entity8273 is distributed heterogeneously. Our goal was to determine the consequences of a lack of either @entity14660 or @entity14661 (using specific knockout @entity19 ) on renal function and on @entity8 . Experiments were performed in @entity14660 (-/-) and @entity14661 (-/-) @entity19 and in their wild-type controls. @entity8273 concentration was measured in the plasma and kidney tissue and its distribution through the different kidney zones was examined by immunohistochemistry. Relative mRNA expressions of @entity14660 , @entity14661 and the 3 main @entity8273 synthases were evaluated by quantitative RT-PCR. RESULTS: Kidney function was not impaired in the knockout @entity19 but they displayed elevated @entity8273 concentrations in the plasma and in the kidney. @entity14660 (-/-) @entity19 presented an accumulation of @entity8273 inside the proximal tubular cells whereas @entity14661 (-/-) @entity19 showed @entity8273 accumulation in the interstitial space. In the cortex and in the outer medulla, @entity14660 mRNA expression was up-regulated in @entity14661 (-/-) @entity19 . From our study we conclude that somatic hyaluronidases are not required for renal function. However, @entity14660 is necessary for the breakdown of intracellular @entity8273 in the cortex, whereas @entity14661 is essential for the degradation of extracellular @entity8273 in all kidney regions.
