Characterization of integrons in multiple antimicrobial resistant XXXX isolates from @entity291 @entity7288 .

@entity4766 @entity15183 @entity7048 @entity14116 @entity7288 @entity59499 @entity58268 @entity2177 @entity5385 @entity51126 @entity73349 @entity2755 @entity43105 @entity206 @entity581 @entity51127 @entity803 @entity291 @entity526
To assess the prevalence of antimicrobial resistance and three classes of integrons in @entity526 (E. coli) strains (n=57) isolated from @entity291 @entity7288 in Inner Mongolia of China, antimicrobial susceptibility and the presence of three types of integrons were characterized. Most isolates were susceptible to ceftiofur, @entity15183 , @entity2177 and @entity14116 , while 57 isolates were all resistant to @entity73349 and trimethoprim. High resistant incidence rates were exhibited to @entity4766 , @entity803 , @entity5385 , @entity7048 , @entity2755 . Forty-six of 57 @entity526 strains were resistant to above 10 antibiotics (80.70%). The integrase gene and gene cassettes of integrons were amplified by PCR. DNA sequencing and analysis were used to identify the genetic content of the integron-variable regions. Neither class II nor class III integron was detected, while 36.8% (n=21) of the isolates were positive for the presence of @entity59499 gene. Analysis of gene cassettes revealed that six gene cassettes were found, which encoded resistance to @entity206 (dhfr, dhfrI, @entity58268 ) and @entity581 ( @entity51126 , @entity51127 , @entity43105 ). Among them, the gene cassette array @entity58268 - @entity43105 was found most prevalent (66.7%). The resistance profile of positive-integron isolates was relatively broad and they were resistant to more than eight antimicrobials (n >=  8). The correlation analysis revealed the incidence of integrons among the isolates were related to the multiple antibiotic resistance profile, indicating integrons play an important role in the dissemination and spread of the antimicrobial resistant strains.
