The effect of chronic XXXX on gingival connective tissue proteoglycans and hyaluronic acid.

@entity1 @entity32 @entity13275 @entity12006 @entity8851 @entity12953 @entity597
Proteoglycans have been isolated and analysed from extracts of normal and chronically inflamed @entity1 gingiva in order to determine the effects of chronic @entity32 on these important soft connective tissue extracellular macromolecules. The @entity13275 content of glycosaminoglycans isolated by papain digestion of normal and inflamed gingiva did not differ significantly. Likewise, electrophoretic analysis revealed that the content of hyaluronic acid, heparan @entity597 , @entity12953 and @entity12006 was similar. The sulfated glycosaminoglycans from both sources eluted from a Sepharose C1-6B column with a Kav of 0.45 (approximate Mr 25,000). However, hyaluronic acid from normal gingiva was predominantly of a large size eluting in the void volume of a Sepharose. CL-6B column, while that isolated form inflamed tissue was mostly a small molecular weight species which eluted in the included volume of a Sepharose CL-6B column. Using dissociative conditions, intact proteoglycans could be more readily extracted from inflamed tissues (90% of the total tissue @entity13275 ) than from normal tissues where only 80% of the total tissue @entity13275 was extractable. Even though @entity8851 -Sephacel ion-exchange chromatography revealed no differences in charge between normal and inflamed gingival proteoglycans, Sepharose CL-4B chromatography revealed more molecular size polydispersity in samples from inflamed tissue than from normal tissue. Taken together, these results indicate that while hyaluronic acid is depolymerized in inflamed tissue, no evidence of sulfated glycosaminoglycan degradation was found. Therefore, the most likely cause for disruption to the molecular integrity of the proteoglycans is via proteolytic alteration to the proteoglycan core protein.
