XXXX GDP-mannose pyrophosphorylase OsVTC1-1 and OsVTC1-3 play different roles in @entity620 synthesis.

@entity620 @entity958 @entity70803 @entity27965 @entity579 @entity7943
UNASSIGNED: @entity70803 pyrophosphorylase (GMPase) catalyzes the synthesis of @entity70803 , which is a precursor for @entity620 ( @entity7943 ) synthesis in plants. The @entity579 genome encodes three GMPase homologs OsVTC1-1, OsVTC1-3 and OsVTC1-8, but their roles in @entity7943 synthesis are unclear. The overexpression of OsVTC1-1 or OsVTC1-3 restored the @entity7943 synthesis of @entity27965 -1 in @entity958 , while that of OsVTC1-8 did not, indicating that only OsVTC1-1 and OsVTC1-3 are involved in @entity7943 synthesis in @entity579 . Similar to @entity958 @entity27965 , the expression of OsVTC1-1 was high in leaves, induced by light, and inhibited by dark. Unlike OsVTC1-1, the expression level of OsVTC1-3 was high in roots and quickly induced by the dark, while the transcription level of OsVTC1-8 did not show obvious changes under constant light or dark treatments. In OsVTC1-1 RNAi plants, the @entity7943 content of @entity579 leaves decreased, and the @entity7943 production induced by light was limited. In contrast, OsVTC1-3 RNAi lines altered @entity7943 synthesis levels in @entity579 roots, but not in the leaves or under the light/dark treatment. The enzyme activity showed that OsVTC1-1 and OsVTC1-3 had higher GMPase activities than OsVTC1-8 in vitro. Our data showed that, unlike in @entity958 , the @entity579 GPMase homologous proteins illustrated a new model in @entity7943 synthesis: OsVTC1-1 may be involved in the @entity7943 synthesis, which takes place in leaves, while OsVTC1-3 may be responsible for @entity7943 synthesis in roots. The different roles of @entity579 GMPase homologous proteins in @entity7943 synthesis may be due to their differences in transcript levels and enzyme activities.
