Growth effects of protein hormones on cultured XXXX ovarian surface epithelial cells.

@entity1 @entity95 @entity665 @entity1014 @entity2670 @entity1098
Throughout life, the @entity2670 ( @entity2670 ) undergoes morphogenetic changes that may be hormonally regulated. To investigate this possibility, a population of cells morphologically identical to native @entity2670 cells was isolated from estrous @entity95 with collagenase, unit gravity sedimentation, and trypsin- @entity1098 . Cells were incubated with various concentrations of protein hormones in serum-rich medium or in a chemically defined medium containing fibronectin. @entity665 was added 24 h before interruption of cultures. Growth-promoting effects of tested hormones were more pronounced and consistent in serum-free cultures. Under these conditions, @entity1 chorionic gonadotropin (10,000 mIU/ml) caused a 2.8-fold increase in cell number and a 3.4-fold stimulation of @entity1014 incorporation. Luteinizing hormone (NIAMDD-oLH-24, 1.0 micrograms/ml) and follicle-stimulating hormone (NIADDK-oFSH-16, 1.0 micrograms/ml) produced, respectively, a 1.7-fold and 1.5-fold increase in cell proliferation, and over 1.4-fold and 1.3-fold stimulations of @entity1014 uptake. When used together, no growth stimulation by these gonadotropins was seen. Slight but significant increases in cell number (1.4-fold) and in radiolabel incorporation (1.3-fold) were observed with prolactin (NIADDK-oPrl-16, 10 ng/ml). These data indicate that some protein hormones promote the growth of @entity2670 cells. This property may be important in regulating these cells during normal and pathologic states.
