Protective effect of XXXX on the immune function of dendritic cells after ultraviolet B irradiation.

@entity2937 @entity7938 @entity2936 @entity3089 @entity3090 @entity98 @entity3646 @entity688 @entity2841 @entity4107
AIM: To investigate the protective effect of @entity3089 ( @entity3090 ) on the immune function of dendritic cells (DCs) after ultraviolet B irradiation (UVB) and its underlying mechanisms. METHODS: The monocytes were isolated from peripheral blood and cultivated into DCs with cytokines, such as @entity2841 ( @entity2841 ) and @entity3646 . DCs were harvested after cultivation for 7 days and subjected to irradiation with different dosages of UVB. Then, 200 microg/mL of @entity3090 was added in certain groups 24 h before irradiation. DCs simply treated with UVB or treated with both UVB and @entity3090 were co-cultured with lymphocytes, and Mono-nuclear cell direct cytotoxicity ( @entity98 ) assay was used to detect the ability of DCs to stimulate proliferation of lymphocytes. Surface markers @entity7938 , @entity2937 , human @entity4107 (HLA-DR), and @entity2936 were detected using flow cytometry, and the levels of @entity688 and IL-12 secreted from DCs 24 h after cultivation were measured using ELISA. RESULTS: UVB irradiation was able to inhibit the ability of DCs to stimulate the proliferation of lymphocytes and surface expressions of @entity7938 , @entity2937 , HLA-DR, and @entity2936 on DCs in a dose-dependent manner. The inhibition rate of DCs was improved to some extent after treatment with 200 microg/mL of @entity3090 . UVB showed no significant influence on the secretion of @entity688 and IL-12 from DCs, while @entity3090 was able to down-regulate the secretion level of IL-12 and up-regulate that of @entity688 . CONCLUSIONS: @entity3090 can antagonize the inhibitory effect on DCs induced by UVB irradiation. This function has some relationship with its protecting effect of the expression of the costimulating molecules on the surface of DCs and the secretion level of @entity688 and IL-12.
