Determination of XXXX in @entity1 plasma by a sensitive HPLC-ESI-MS/MS method: application for a pharmacokinetic study using nasal spray formulation.

@entity1 @entity2995 @entity5935 @entity6190 @entity1626 @entity2713
A liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantitation of @entity2713 in @entity1 plasma after nasal spray application was developed and validated. @entity5935 was used as internal standard (IS). The analytes were extracted by a liquid-liquid procedure and separated on a Zorbax Eclipse XDB C(18) column with a mobile phase composed of 2 mM aqueous @entity6190 pH 3.2 and @entity1626 (55:45). Selected reaction monitoring was performed using the transitions m/z 435  >  415 and m/z 393  >  373 to quantify @entity2995 and @entity5935 , respectively. Calibration curve was constructed over the range of 20-2000 pg/ml for @entity2995 . The lower limit of quantitation was 20 pg/ml. The mean RSD values were 4.6% and 5.7% for the intra-run and inter-run precision, respectively. The mean accuracy value was 98.5% and a recovery rate corresponding to 97.5% was achieved. No matrix effect was detected in the samples. The validated method was successfully applied to determine the plasma concentrations of @entity2995 in healthy volunteers, in a pharmacokinetic study with nasal spray formulation.
