XXXX expressing lipoprotein lipase in adipose tissue. Absence of the proximal 3'-untranslated region causes translational upregulation.

@entity2298 @entity14694 @entity844 @entity19 @entity5523 @entity731 @entity2661 @entity413
Lipoprotein lipase ( @entity14694 ) is a key enzyme in lipoprotein and adipocyte metabolism. Defects in @entity14694 can lead to @entity2298 and the subsequent development of @entity731 . The mechanisms of regulation of this enzyme are complex and may occur at multiple levels of gene expression. Because the 3'-untranslated region (UTR) is involved in @entity14694 translational regulation, @entity19 were generated with adipose tissue expression of an @entity14694 construct either with or without the proximal 3'-UTR and driven by the aP2 promoter. Both transgenic @entity19 colonies were viable and expressed the transgene, resulting in a 2-fold increase in @entity14694 activity in white adipose tissue. Neither @entity19 colony exhibited any obvious phenotype in terms of body weight, plasma lipids, @entity413 , and non-esterified @entity844 levels. In the @entity19 expressing @entity5523 with an intact 3'-UTR, @entity5523 mRNA expression approximately paralleled @entity5523 activity. However in the @entity19 without the proximal 3'-UTR, @entity5523 mRNA was low in the setting of large amounts of @entity5523 protein and @entity14694 activity. In previous studies, the 3'-UTR of @entity14694 was critical for the inhibitory effects of constitutively expressed hormones, such as thyroid hormone and @entity2661 . Therefore, these data suggest that the absence of the 3'-UTR results in a translationally unrepressed @entity14694 , resulting in a moderate overexpression of adipose @entity14694 activity.
