Developmental changes of XXXX in ovine fetal fluids.

@entity7792 @entity1522 @entity2227 @entity1160 @entity2228 @entity535 @entity563 @entity493 @entity369
We recently reported an unusual abundance of @entity369 (4-6 mM) in porcine allantoic fluid during early gestation. However, it is not known whether such high concentrations of @entity369 are unique for porcine allantoic fluid or whether they represent an important physiological phenomenon for mammals. The present study was conducted to test the hypothesis that @entity369 is also the most abundant @entity535 in ovine allantoic fluid. Allantoic and amniotic fluids, as well as fetal and maternal plasma samples, were obtained from ewes between Days 30 and 140 of gestation. @entity2228 was the most abundant @entity535 in maternal uterine arterial plasma, representing approximately 25% of total @entity535 . @entity493 , @entity1160 , @entity2228 , plus @entity563 contributed approximately 50% of total @entity535 in fetal plasma. Fetal:maternal plasma ratios for @entity1522 varied greatly, being less than 1 for @entity2227 during late gestation, 1.5-3 for most @entity1522 throughout gestation, and greater than 10 for @entity563 during late gestation. Marked changes were observed in @entity535 concentrations in amniotic and allantoic fluids associated with conceptus development. Concentrations of @entity493 , @entity7792 , and @entity1160 in allantoic fluid increased by 20-, 34-, and 18-fold, respectively, between Days 30 and 60 of gestation and were 24.7, 9.7, and 23.5 mM, respectively, on Day 60 of gestation (compared with 0.8 mM @entity369 ). Remarkably, @entity493 , @entity7792 , plus @entity1160 accounted for approximately 80% of total @entity535 in allantoic fluid during early gestation. @entity563 (16.5 mM) contributed approximately 60% of total @entity535 in allantoic fluid on Day 140 of gestation. These novel findings of the unusual abundance of traditionally classified nonessential @entity1522 in allantoic fluid raise important questions regarding their roles in ovine conceptus development.
