[Significance of methylation status of @entity11178 gene in differential diagnosis for XXXX ].

@entity1 @entity11178 @entity4289 @entity2150 @entity2590 @entity1503 @entity309
It is hard to discriminate @entity2590 ( @entity4289 ) from many benign @entity1503 . To identify the methylation status of @entity11178 gene in @entity4289 , the methylation specific PCR (MS-PCR) and reverse transcription-PCR (RT-PCR) were applied to detect the @entity4289 cell line MUTZ-1, bone marrow of a healthy donor and an @entity2150 @entity1 . MS-PCR was also employed to detect the bone marrow of 72 @entity1 with benign @entity1503 , 35 @entity309 @entity1 , and 20 @entity4289 -like @entity1 . The results showed that @entity4289 cell line MUTZ-1 displayed complete methylation of @entity11178 promoter without mRNA expression. Inversely, a @entity1 with benign @entity1503 and a donor with normal bone marrow showed complete unmethylation of this gene with unaffected mRNA expression. No @entity11178 promoter methylation was detected in @entity1 with benign @entity1503 , while aberrant hypermethylation of @entity11178 gene promoter were found in 48.6% (18/37) of @entity309 @entity1 . The positive rate of @entity11178 methylation in @entity309 @entity1 was higher than that in @entity1 with benign @entity1503 (p < 0.05). Seven suspected @entity4289 @entity1 manifested hypermethylation status of @entity11178 gene (7/20), 2 were followed up for 1 year and transformed into @entity4289 . It is concluded that relatively high hypermethylation rate of @entity11178 promoter is observed in @entity309 , and no methylation in @entity1 with benign @entity1503 . Therefore, @entity11178 gene hypermethylation may be served as a useful epigenetic marker in the differential diagnosis for @entity4289 .
