Single-cell mechanics and @entity460 signalling in organotypic slices of XXXX myometrium.

@entity398 @entity1 @entity548 @entity3465 @entity1266 @entity2918 @entity854 @entity460 @entity141
UNASSIGNED: Elucidation of cellular mechanisms regulating myometrial contractility is crucial for improvement in management of many @entity854 , such as @entity3465 , uterine @entity1266 and post-partum @entity548 . Myometrial contractions are triggered by periodic synchronous rises in intracellular @entity460 concentration ([ @entity141 ]i) elicited by spontaneously generated action potentials propagating throughout the entire myometrium. During labour, hormones like @entity398 and @entity2918 potentiate uterine contractions by increasing their duration, strength and frequency. The most informative approach to studying the mechanisms underlying hormonal modulation of uterine contractility is to record [ @entity141 ]i responses to hormones in intact myometrial samples that have not been subjected to enzymatic treatment for cell isolation or cell culture conditions. However, the spatio-temporal resolution of such recording is limited due to the motion artifacts occurring in contracting tissue. Here we describe the application of our newly developed motion correction algorithm to investigate the [ @entity141 ]i dynamics in control and @entity398 stimulated slices of @entity1 myometrium on a cellular level. We present evidence that @entity398 induces asynchronous [ @entity141 ]i oscillations in individual myocytes within intact myometrium which are similar to those observed in cultured cells. The oscillations occur between synchronous action potential-driven [ @entity141 ]i transients but appear to be unrelated to contractions. Furthermore, the @entity398 -triggered [ @entity141 ]i oscillations wane within 30-50min of hormone application, while the action potential induced [ @entity141 ]i transients remain augmented. We conclude that @entity398 -induced [ @entity141 ]i oscillations are not relevant to the acute regulation of myometrial contractility but may play a role in longer-term regulatory processes, for example, by triggering gene expression.
