Real Time Metastatic Route Tracking of Orthotopic XXXX -GFP @entity1 Prostate Cancer Using Intravital Imaging.

@entity1 @entity263 @entity5 @entity19 @entity3 @entity5049
UNASSIGNED: The cellular basis of @entity3 is poorly understood. An important step to understanding this process is to be able to visualize the routes by which @entity5 cells migrate from the @entity5 to various distant sites to eventually form @entity3 . Our laboratory previously developed single-cell in vivo imaging using fluorescent proteins to label @entity5 cells. In the present study, using @entity5049 @entity1 @entity263 cells labeled with green fluorescent protein (GFP) and @entity5 transplantation, we have imaged in live @entity19 various highly diverse routes by which @entity5049 cells metastasize superiorly and inferiorly to distant sites, including in the portal area, stomach area, and urogenital system. Imaging began at day 9, at which time distant @entity3 had already occurred, and increased at each imaging point at days 10, 13, 14, and 16. Metastatic cells were observed migrating superiorly and inferiorly from the @entity5 as well as in lymphatic channels and trafficking in various organ systems demonstrating that @entity5049 has multiple metastatic routes similar to hormone-independent @entity263 in the clinic. J. Cell. Biochem. 117: 1027-1032, 2016.    2015 Wiley Periodicals, Inc.
