The effect of @entity270 on the response of XXXX bladder smooth muscle to @entity273 .

@entity274 @entity270 @entity1494 @entity63 @entity4338 @entity409 @entity95 @entity273 @entity519 @entity1881 @entity1234 @entity5402 @entity1865
There is increasing evidence that @entity63 , reperfusion, and the generation of free radicals are major etiological factors in the progression of @entity1494 after partial outlet obstruction. In vitro studies demonstrated that the magnitude of @entity409 following exposure of bladder smooth muscle to @entity519 followed by re-oxygenation was related to the level of lipid peroxidation indicating that membrane lipid peroxidation participated in the contractile failure induced. Recent studies demonstrated that incubation of isolated strips of bladder smooth muscle with @entity273 ( @entity274 ) result in progressive @entity409 and is associated with progressive increases in @entity1865 (peroxidation product). The current study investigates if feeding @entity95 a diet high in @entity270 protects the bladder from the effects of in vitro @entity274 . Sixty-four male New Zealand White @entity95 were separated into two groups: The @entity95 in group 1 were fed a normal diet (28 @entity95 ) whereas the @entity95 in group 2 were placed on a diet enriched with @entity1881 (36 @entity95 ). After 3 weeks on the normal or high E diet, each @entity95 was anesthetized and the bladder excised and cut into 6 isolated strips of bladder detrusor. Each strip was mounted in individual 15 ml baths containing oxygenated Tyrode's solution. The contractile responses to field stimulation (FS), @entity5402 . and @entity4338 were determined. The strips were washed and exposed to one of the following concentrations of @entity273 ( @entity274 ): 0% (control), 0.0625, 0.125, 0.25, 0.5, 1.0 and 3.0% for a period of 1 h. At the end of the hour each strip was washed free of @entity274 and a second set of contractile responses were performed and compared to the first set. At the end of the experiment, each strip was frozen and stored at -70 degrees C for analysis of @entity1234 ( @entity1865 ) as a measure of peroxidation. In both groups, @entity274 produced similar dose dependent decreases in the contractile responses to all forms of stimulation. In the normal-diet group @entity274 produced a dose dependent increase in @entity1865 formation, whereas in the high E group there were no increases in @entity1865 at any concentration of @entity274 . Feeding @entity95 a diet high in @entity270 protected the bladder smooth muscle from peroxidation, but had no significant effect on the @entity409 mediated by direct incubation with @entity274 .
