MicroRNAs are differentially deregulated in mammary XXXX .

@entity11216 @entity5 @entity10015 @entity16105 @entity10017 @entity983
AIMS: MicroRNAs (miRs) have been shown to play important roles in @entity5 progression. Their expression pattern can be useful for @entity5 classification. However, little is known about miRs in mammary phyllodes tumours (PT). METHODS AND RESULTS: In this study, polymerase chain reaction (PCR)-based @entity11216 profiling was performed in a small PT cohort to identify deregulated miRs in malignant PT. The purported roles and targets of these miRs were further validated. Unsupervised clustering of @entity11216 expression profiling segregated PT into different grades, implicating the @entity11216 profile in PT classification. Among the deregulated miRs, @entity10017 , @entity16105 and @entity10015 were validated to be higher in malignant than in lower-grade PT in the independent cohort by quantitative PCR (qPCR) (P   <=  0.032). Their expression correlated with some of the malignant histological features, including high stromal cellularity, nuclear pleomorphism and mitosis. Subsequent analysis of their downstream proteins, namely @entity983 for @entity10017 / @entity10015 and Rb for @entity16105 , also showed an independent significant negative association between @entity11216 and protein expression. CONCLUSIONS: Differential expression of miRs in PT could be useful in diagnosis and grading of PT. Their deregulated expression, together with the altered downstream targets, implicated their active involvement in PT malignant transformation.
