Transfer of XXXX into @entity466 @entity19 . The pathogenetic implications of T cell populations oligoclonally expanding in the @entity309 .

@entity1 @entity19 @entity1373 @entity5168 @entity309 @entity466
To investigate the pathogenicity of T cells infiltrating in the @entity309 , mononuclear cells (MNC), predominantly T cells, isolated from either synovial fluid or synovial tissues of the @entity1 with @entity309 were transferred into @entity466 ( @entity466 ) @entity19 by intraarticular injections. According to our observations in this experimental system, @entity1 with @entity309 could be classified into at least two groups. In one group of @entity1 , the infiltrating MNC induced @entity1373 in the recipient @entity466 @entity19 (the positive group). Whereas, in the other group no @entity1373 was observed (the negative group). The induction of @entity1373 observed in the positive group was prevented by an anti-human @entity5168 antibody ( @entity19 ), indicating T cell mediation. Analysis of T cell receptor (TCR) V beta usage by reverse transcriptase polymerase chain reaction in the infiltrating MNC transferred into @entity466 @entity19 revealed a marked skew towards the preferential use of certain V beta genes, which was not seen in the peripheral blood MNC, in only the positive group. The patterns of TCR/V beta skew were not uniform among the @entity1 . The analysis of the PCR-amplified genes of such skewed TCR/ V beta by single strand conformational polymorphism showed distinct bands, indicating that the T cell populations expanding in @entity309 of the positive group were oligoclonal. Furthermore, the enrichment of the T cell populations expressing such skewed TCR/V beta by in vitro stimulation of peripheral blood MNC of the @entity1 with the relevant superantigen enabled the induction of @entity1373 in the @entity466 @entity19 . These results suggest that the pathogenic T cells could be activated locally in @entity309 by certain antigens in some, but not in all @entity1 with @entity309 .
