The identification and characterization of XXXX , a gene required for sporulation and for expression of the @entity167 dehydrogenase II isozyme from @entity375 .

@entity27733 @entity167 @entity5910 @entity48268 @entity505 @entity15517 @entity375 @entity6085 @entity413
The @entity167 dehydrogenase II isozyme (enzyme, ADHII; structural gene, @entity27733 ) of the @entity375 , @entity375 , is under stringent @entity505 catabolite control. This cytoplasmic isozyme exhibits negligible activity during growth in media containing fermentable @entity505 sources such as @entity413 and is maximal during growth on nonfermentable @entity505 sources. A recessive mutation, @entity48268 -1, and possibly two other alleles at this locus, were selected for their ability to decrease Ty-activated @entity27733 -6c expression. The @entity48268 -1 mutation led to decreased ADHII activity in both @entity27733 -6c and @entity27733 + strains, and to decreased levels of @entity27733 mRNA. Ty transcription and the expression of two other @entity505 catabolite regulated enzymes, isocitrate lyase and @entity5910 dehydrogenase, were unaffected by the @entity48268 -1 mutation. @entity48268 -1/ @entity48268 -1 strains were defective for sporulation, indicating that @entity48268 mutations may have pleiotropic effects. The sporulation defect was not a consequence of decreased @entity6085 activity. Since the @entity27733 -6c mutation is due to insertion of a 5.6-kb Ty element at the TATAA box, it appears that the @entity48268 +-dependent ADHII activity required @entity27733 sequences 3' to or including the TATAA box. The @entity27733 upstream activating sequence (UAS) was probably not required. The @entity48268 locus was unlinked to the @entity15517 gene which encodes another trans-acting element required for @entity27733 expression.
