Polychromatic flow cytometry analysis of @entity2280 + hematopoietic stem cells in cryopreserved early preterm XXXX cord blood samples.

@entity1 @entity1262 @entity7403 @entity5439 @entity10430 @entity1195 @entity17341 @entity2279 @entity5399 @entity7801 @entity8282 @entity3053 @entity2393 @entity2467 @entity2280
During the last decades, extended characterizations were performed of @entity1 full-term cord blood ( @entity17341 ) cells, but little information is available on @entity1 early preterm cord blood (hEPCB) hematopoietic stem cells (HSCs). In our study, we analyzed by flow cytometry 19 hEPCB and 17 @entity17341 samples. First, we observed that the percentage of @entity2280 (Pos) @entity1195 (Dim) cells was higher in hEPCB compared with @entity17341 and that it decreased during 16th-20th week of pregnancy. Within the @entity2280 (Pos) @entity1195 (Dim) population, we examined the expression of @entity2393 , @entity2279 , @entity3053 , @entity5399 , @entity2467 , @entity5439 , @entity7801 , @entity7403 , @entity1262 , and @entity10430 . We found that @entity7801 intensity and @entity1262 (Pos) cells percentage were lower in hEPCB compared with @entity17341 . As to @entity3053 , we observed that hEPCB samples were richer in undifferentiated @entity2280 (Pos) @entity1195 (Dim) @entity3053 (Neg) HSCs compared with @entity17341 counterparts. We also compared the expression of the above-mentioned molecules in undifferentiated and committed HSCs residing in hEPCB and @entity17341 . In particular, although @entity2280 (Pos) @entity1195 (Dim) @entity3053 (Pos) HSCs from both hEPCB and @entity17341 expressed relatively higher amounts of @entity2393 , @entity8282 , and @entity7801 compared with @entity2280 (Pos) @entity1195 (Dim) @entity3053 (Neg) cells, a higher expression of @entity2279 was restricted to @entity2280 (Pos) @entity1195 (Dim) @entity3053 (Pos) cells from hEPCB samples, and a higher expression of @entity2467 was demonstrated in @entity2280 (Pos) @entity1195 (Dim) @entity3053 (Pos) cells from @entity17341 samples. Moreover, our data showed that @entity2280 (Pos) @entity1195 (Dim) cell population from hEPCB displayed higher percent of undifferentiated @entity3053 (Neg) @entity5439 (Pos) cells compared with @entity17341 samples. Finally, analyzing monocytes and lymphocytes within the two samples, we observed that T-cell percentages were higher in @entity17341 , whereas B-cell percentages were higher in hEPCB. We, therefore, studied the B-cell lineage maturation and found a higher percent of pro-B and pre-B cells in hEPCB compared with @entity17341 samples. Taken together, these results evidence the hematopoietic peculiarity of hEPCB, potentially useful for highlighting early steps of @entity1 hematolymphopoiesis as well as for developing novel strategies of stem cell-based therapy.
