Immunohistological phenotyping of thyroid infiltrating lymphocytes in @entity2743 and XXXX .

@entity2621 @entity1669 @entity4755 @entity2743
Subsets of lymphocytes in the thyroid were immunophenotyped by their surface antigens in frozen tissues of @entity2621 and @entity2743 . Using triple layer immunoperoxidase staining (IP), monoclonal antibodies (T3, Leu 3, T8, anti-Tac and @entity4755 ) were employed to detect markers of T cell subsets, activated T cells, and a natural killer associated antigen. B cells were identified by 2 step IP with anti-IgD antisera. Excluding those cells forming lymphoid follicles, the density of lymphocytes infiltrating between thyroid epithelial cells was much higher in @entity2621 than in @entity2743 . However, relative proportions of subsets were similar in both diseases. Most of the infiltrating cells were T3 positive T cells (T3+), with more T8+ (suppressor/cytotoxic T) than Leu 3+ (inducer/helper T). Some @entity4755 + were occasionally seen, but surface IgD positive mature B cells (IgD+) were almost absent. In contrast, IgD+ cells were densely aggregated in primary lymphoid follicles and mantle zones of secondary follicles. In these regions, Leu 3+ cells were about twice as frequent as T8+ cells. Some @entity4755 + and scarce Tac+ cells were also found. The present study indicates a major involvement of immunoregulatory T cells in @entity1669 , and also suggested intrathyroidal maturation of B cells.
