The induction of lipid peroxidation during the acute oxidative stress response induced by intratracheal instillation of fine crystalline @entity3440 particles in XXXX .

@entity137 @entity19413 @entity22456 @entity3758 @entity358 @entity880 @entity3440 @entity72015 @entity35
UNASSIGNED: Crystalline @entity3440 ( @entity3758 ) is an important material for industry but is considered potentially carcinogenic. Inhalation of a crystalline @entity3758 aerosol may contribute to serious @entity358 . Crystalline @entity3758 particles are commonly used as a positive control in @entity137 assays of particulate materials (e.g. nanoparticles). Crystalline @entity3758 induces oxidative stress resulting in lipid peroxidation, but the acute oxidative stress response in the lung is not well understood. Lipid peroxidation during the acute stage of oxidative stress after instillation of crystalline @entity3758 into @entity35 was examined by bronchoalveolar lavage fluid (BALF) analysis. The levels of 8-iso-prostaglandin F2a and @entity19413 ( @entity72015 ) in the BALF were measured using liquid chromatography coupled to quadrupole mass spectrometry. The concentration of the antioxidant protein @entity22456 ( @entity22456 ) in the BALF was determined using enzyme-linked immunosorbent assay. Intratracheal instillation of crystalline @entity3758 increased the level of HODE and @entity22456 in BALF at 24 h after administration. The levels of HODE and @entity22456 returned to baseline at 72 h after instillation. @entity880 dehydrogenase leakage was observed only after 1 h instillation. These results suggest that the contribution of oxidative stress to the @entity358 of crystalline @entity3758 is minimal in the early acute stage after exposure.
