Cell-Autonomous Brown-Like Adipogenesis of Preadipocytes from @entity995 Haploinsufficient XXXX .

@entity12097 @entity39065 @entity844 @entity17776 @entity19 @entity72621 @entity10350 @entity55986 @entity72620 @entity26385 @entity17736 @entity7111 @entity4063 @entity413 @entity23918 @entity34616 @entity1386 @entity995
UNASSIGNED: Mechanisms behind the emergence of brown adipocyte-like (brite or beige) adipocytes within @entity1386 ( @entity1386 ) are of interest. @entity995 protein gene (Rb) haploinsufficiency associates in @entity19 with improved metabolic regulation linked to a greater capacity for @entity844 oxidation and thermogenesis in @entity1386 . We aimed to explain a feasible mechanism of @entity1386 -to-BAT remodeling in this model. Differentiated primary adipocytes and @entity17776 -positive preadipocytes derived from adipose depots of Rb(+/-) @entity19 and wild-type siblings were compared. Primary white Rb(+/-) adipocytes displayed under basal conditions increased @entity413 uptake and an enhanced expression of brown adipocyte-related genes ( @entity12097 , @entity7111 , @entity34616 , @entity10350 , @entity4063 ), but not of purported beige/brite transcriptional markers ( @entity23918 , @entity72620 , @entity26385 , @entity55986 , @entity72621 , @entity39065 ). Lack of induction of beige markers phenocopied results in @entity1386 of adult Rb(+/-) @entity19 . Flow cytometry analysis evidenced an increased number of preadipocytes in @entity1386 depots of Rb(+/-) @entity19 . @entity17776 (+) preadipocytes from @entity1386 of Rb(+/-) @entity19 displayed increased gene expression of several transcription factors common to the brown and beige adipogenic programs ( @entity10350 , @entity12097 , @entity7111 ) and of receptors of bone morphogenetic proteins (BMPs); however, amongst the recently proposed beige markers, only @entity26385 was upregulated. Adult Rb(+/-) @entity19 had increased circulating levels of @entity17736 . These results indicate that preadipose cells resident in @entity1386 depots of Rb(+/-) @entity19 retain an increased capacity for brown-like adipogenesis that appears to be different from beige adipogenesis, and suggest that the contribution of these precursors to the Rb(+/-) adipose phenotype is driven, at least in part, by interaction with @entity17736 pathways. This article is protected by copyright. All rights reserved.
