In vivo bioengineered XXXX based on collagen, matrigel, alginate and agarose hydrogels: a comparative study.

@entity1 @entity43 @entity7738 @entity39 @entity5 @entity19 @entity7
Scaffold-based @entity5 engineering is rapidly evolving the study of @entity5 progression. However, the effects of scaffolds and environment on @entity5 formation have seldom been investigated. In this study, four types of injectable hydrogels, namely, collagen type I, Matrigel, alginate and agarose gels, were loaded with @entity1 @entity43 SKOV3 cells and then injected into @entity19 subcutaneously. The growth of the @entity5 in vitro was also investigated. After four weeks, the specimens were harvested and analyzed. We found that @entity5 formation by SKOV3 cells was best supported by collagen, followed by Matrigel, alginate, control (without scaffold) and agarose in  vivo. The collagen I group exhibited a larger @entity5 volume with increased neovascularization and increased @entity39 compared with the other materials. Further, increased MMP activity, upregulated expression of laminin and fibronectin and higher levels of @entity7738 and @entity7 in the collagen group revealed that the engineered @entity5 is closer to @entity1 @entity43 . In order, collagen, Matrigel, alginate, control (without scaffold) and agarose exhibited decreases in @entity5 formation. All evidence indicated that the in vivo engineered @entity5 is scaffold-dependent. Bioactive hydrogels are superior to inert hydrogels at promoting @entity5 regeneration. In particular, biomimetic hydrogels are advantageous because they provide a microenvironment that mimics the ECM of natural @entity5 . On the other hand, typical features of @entity5 cells and the expression of genes related to @entity5 were far less similar to the @entity5 in vitro, which indicated the importance of culture environment in vivo. Superior to the in vitro culture, @entity19 can be considered satisfactory in vivo 'bioreactors' for the screening of favorable cell vehicles for @entity5 engineering in vitro.
