Energy metabolism in human erythrocytes. II. Effects of XXXX depletion.

@entity972 @entity6297 @entity6298 @entity16055 @entity1372 @entity38398 @entity920 @entity880 @entity33168 @entity2388 @entity7832 @entity413 @entity855
Normal red cells were incubated in the absence of @entity413 to develop a system in which total @entity855 ( @entity855 ) turnover could be assessed. After 1 hr, the @entity38398 pool had been completely consumed. Thereafter, the metabolism of @entity6297 ( @entity6298 ) to @entity2388 and @entity880 was the sole significant source of @entity855 synthesis.10(-3)M @entity33168 , which did not enter the cells, diminished @entity855 utilization by more than 50%. This could be only partially attributed to the inhibition by @entity920 of residual acylation and cation pumping, which were already reduced by @entity413 depletion. Other membrane enzymes, which presumably function in the maintenance of membrane integrity, must, therefore, use a significant portion of erythrocyte @entity855 . The behavior of @entity413 -depleted red cells with respect to cation transport was complex. The addition of @entity7832 did not decrease @entity855 utilization in these red cells. Ouabain inhibitable @entity972 influx was nearly normal after @entity38398 depletion, but total @entity972 influx was decreased. In contrast, the ouabain inhibitable @entity1372 efflux was markedly reduced after @entity38398 depletion, although the concentration of @entity855 was 70% of normal. The dissociation of monovalent cation pumping suggests that the energy for active @entity1372 transport is derived from a specific source (such as the @entity855 produced by the @entity16055 kinase reaction) distinct from that for @entity972 transport.
