Analysis of microRNA expression profiling identifies microRNA-503 regulates metastatic function in hepatocellular XXXX cell.

@entity5 @entity1 @entity157 @entity26607
BACKGROUND AND OBJECTIVES: Metastasis of @entity5 is a complex process that involves multiple alterations. Recent evidence indicates that small non-protein coding RNA molecules (miRNAs) might be involved in @entity5 -related processes in @entity1 . This study was to systematically investigate the differentially expressed miRNAs during metastasis in @entity157 ( @entity157 ) using microarray technology. METHODS: The differentially expressed miRNAs between HCCLM3 and MHCC97-L, two @entity157 cell lines with differently metastatic potentials were displayed using microarray technology. The expression of @entity26607 was verified by the real-time quantitative polymerase chain reaction. In addition, the lentivirus-delivered system for expressing @entity26607 in HCCLM3 cells was employed to investigate whether @entity26607 was involved in invasive phenotype of @entity157 cell. RESULTS: Our study built a metastasis-related miRNAs expression profiling, which includes 327 miRNAs expressed differentially between HCCLM3 and MHCC97-L cell lines. Furthermore, expression of @entity26607 by lentivirus-delivered system in HCCLM3 cell was established successfully. Our results showed that @entity26607 induces a G1 arrest and decreased proliferation for HCCLM3 cell (P < 0.05). In addition, @entity26607 inhibits migration and invasion of HCCLM3 cell in vitro (P < 0.05). CONCLUSIONS: This study described a metastasis-related miRNAs expression profiling and revealed @entity26607 regulating metastatic function in @entity157 cell.
