Effect of Nourishing "Yin" Removing "Fire" Chinese Herbal Mixture on Hypothalamic Mammalian Target of Rapamycin Expression during Onset of Puberty in Female XXXX .

@entity53 @entity3470 @entity1058 @entity35 @entity4361
Aim. The present study aims to investigate the effects of nourishing "Yin" removing "Fire" (NYRF) @entity1058 on puberty onset and hypothalamic @entity3470 expression in female @entity35 . Materials and Methods. Forty female 20-day-old @entity35 were randomly divided into @entity1058 ( @entity1058 ) and @entity4361 ( @entity4361 ) groups. @entity35 in @entity1058 and @entity4361 were treated with NYRF mixture and @entity4361 , respectively, from d22. @entity35 in each group were sacrificed on d28, d31, and d34. Serum luteinizing hormone (LH), follicle stimulating hormone (FSH), and @entity53 (E2) levels were analyzed by ELISA. Hypothalamic @entity3470 mRNA expression levels were determined by RT-PCR and hypothalamic p- @entity3470 protein levels were assayed by western blot. Results. The vaginal opening time in @entity1058 group was significantly delayed (P < 0.05). On d31, in comparison with @entity4361 group, the coefficients of uteri and ovaries, levels of serum LH and E2, and the expression levels of hypothalamic @entity3470 mRNA and p- @entity3470 protein were significantly lower in @entity1058 group (P < 0.05). Conclusion. The mechanism by which the nourishing "Yin" removing "Fire" @entity1058 delays puberty onset may be associated with the inhibition of the hypothalamic @entity3470 signaling.
