Implantation of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells in Foot Fat Pad in XXXX .

@entity35 @entity1 @entity1386 @entity158
BACKGROUND: The foot fat pad (FFP) bears body weight and may become a source of foot @entity158 during aging. This study investigated the regenerative effects of autologous @entity1386 ( @entity1386 ) in the FFP of @entity35 . METHODS: Fat tissue was harvested from a total of 30 male @entity35 for isolation of @entity1386 . The cells were cultured, adipogenic differentiation was induced for 1 week, and the @entity1386 were labeled with fluorescent dye before injection. @entity1386 (5 * 10(4) in 50   L of saline) were injected into the second infradigital pad in the right hindfoot of the @entity35 of origin. Saline only (50   L) was injected into the corresponding fat pad in the left hind paw of each @entity35 . @entity35 (n = 10) were euthanized at 1, 2, and 3 weeks, and the second infradigital fat pads were dissected for histologic examination. RESULTS: The fluorescence-labeled @entity1386 were present in the foot pads throughout the 3-week experimental period. On histologic testing, the area of fat pad units (FPUs) in the fat pads that received AT-MSC injections was greater than that in the control fat pads. Although the thickness of septae was not changed by AT-MSC injections, the density of elastic fibers in the septae was increased in the fat pads with @entity1386 . CONCLUSION: In this short-term study, the @entity1386 largely survived and might have stimulated the expansion of individual FPUs and increased the density of elastic fibers in the FFP in this @entity35 model. CLINICAL RELEVANCE: These data support the development of stem cell therapies for age-associated degeneration in FFP in @entity1 .
