[Specific anti-tumor immunity and its cross-reaction induced by antigen peptide mixture prepared from different XXXX cell lines].

@entity1778 @entity1619 @entity137 @entity2285
OBJECTIVE: To investigate the specific antitumor immunity induced by antigen peptide mixture prepared from different @entity1619 cells and the cross-reaction among the mixtures of different cell lines. METHODS: Antigen peptide mixtures were prepared from different @entity1778 cell lines and then bound with @entity2285 in vitro. The activation and proliferation of peripheral blood mononuclear cell (PBMC) were observed after the stimulation by different @entity2285 -peptide complexes. The @entity137 of such activated PBMCs to different target cells was assayed. RESULTS: The antigen peptides from different @entity1778 cell lines were mixed ones, which could activate PBMC effectively with @entity2285 and stimulate the activated PBMC to proliferate. The proliferative PBMC had specific @entity137 to the corresponding @entity1778 cells. To Hut-78 cell, Molt-4 cell and Jurkat cell, the @entity137 of PBMC activated by either Hut78-peptides or Molt-4-peptides was significantly stronger than that of PBMC activated by HL-60-peptides (P < 0.05). The @entity137 to Jurkat cell of PBMC activated by Hut78/Molt-4-peptides was significantly stronger than that of PBMC activated by Hut78-peptides or Molt-4-peptides alone (P < 0.05). CONCLUSION: Antigen peptide mixture from @entity1619 cells is able to induce specific antitumor immunity. There is a cross-reactivity among antigen peptide mixtures from different @entity1619 cell lines, with the more crossed antigen peptides obtained from the mixtures of different antigen peptides from different @entity1619 cell lines, which suggests that the antigen peptide mixture with broad antigenic spectrum could possibly be prepared by using multiple @entity1778 cell lines.
