Optimal time for post-mortem heparinization in XXXX lung transplantation with non-heart-beating donors.

@entity63 @entity235 @entity6950 @entity972 @entity413 @entity1167 @entity4337
BACKGROUND: We previously reported that post-mortem heparinization by closed-chest cardiac massage is beneficial in lung transplantation from non-heart-beating donors by preventing formation of microthrombi. In this study, we evaluated the optimal time for post-mortem heparinization in @entity235 lung transplantation from non-heart-beating donors. METHODS: Left lung transplantation was performed in 25 weight-matched pairs of mongrel @entity235 . Donors were killed with an intravenous injection of @entity4337 and left at room temperature for 2 hours. The cadaver donors were assigned randomly to one of five study groups. In Group H0, @entity6950 (1,000 U/kg) was given intravenously before @entity1167 . In Groups H10, H30, H45 and H60, @entity6950 (1,000 U/kg) was given intravenously 10, 30, 45 and 60 minutes after @entity1167 , respectively, followed by closed-chest cardiac massage for 2 minutes. After 2 hours of @entity1167 , donor lungs were flushed with low- @entity972 dextran @entity413 solution and preserved for 60 minutes. After left lung allotransplantation, the right pulmonary artery was ligated, and recipient animals were followed up for 3 hours. Uni- and multivariate repeat analyses were utilized for statistical assessment. RESULTS: After transplantation, gas exchange was significantly worse in Groups H45 and H60 than in Groups H0, H10 and H30. Thrombin/anti-thrombin III complex concentration during warm @entity63 was significantly higher in Groups H30, H45 and H60 than in Groups H0 and H10. CONCLUSIONS: The optimal time for post-mortem heparinization in lung transplantation from non-heart-beating donors is approximately 30 minutes after @entity1167 .
