A new @entity11 model in XXXX : the C6 spheroid implantation technique permeability and vascular characterization.

@entity39 @entity5 @entity11 @entity816 @entity35 @entity463
The C6 spheroid implantation @entity11 model is a simple, easily reproduced model for @entity11 in which C6 @entity816 cells are grown in vitro as spheroids and subsequently implanted into the brains of @entity35 hosts. This report describes the growth, histology, vessel architecture and vascular permeability of the resulting @entity5 . The appearance of the @entity5 was investigated by light and electron microscopy, and by using the alkaline phosphatase technique. The leakage of tracer was measured from vessels in the @entity5 and peritumoral area at various times during @entity5 development. The spheroid implant produces a fully vascularized, rapidly growing @entity5 with many of the characteristics of @entity463 multiforme, from an avascular focus of neoplastic cells. The major advantage of this model is that the @entity5 grow in a spheroidal fashion and the @entity5 -brain interface can be easily located. Many of the important events in the process of vascularization take place at the @entity5 -brain interface. Two distinctive vascular events appear to occur simultaneously: proliferation of blood vessels and their growth into the @entity5 mass so that they develop into typical, permeable @entity5 vessels, and migration of @entity5 cells along normal vessels into the surrounding brain. Tumor vessels were permeable to the tracer Evans Blue (EB) from the earliest days of ingrowth. Leakage of the EB increased as the @entity5 increased in size, but eventually leakage plateaued as @entity5 developed @entity39 centers. It is well known that the structural and permeability characteristics of vessels associated with the @entity5 affect @entity5 growth. This model will be useful for a number of proposed studies including assessment of various clinical therapies on @entity5 growth and development, and more specifically, quantitative analysis of the vascularization process in @entity5 .
