Characterization of @entity2184 cell lines that grow as xenografts in XXXX eyes.

@entity1 @entity5 @entity1711 @entity95 @entity406 @entity205 @entity2184 @entity1710 @entity27521 @entity320
Two cell lines, @entity27521 and @entity27521 , were established from biopsied specimens of @entity320 of spindle B and mixed cell type morphologies. Both cell lines were phenotypically malignant. Karyotypic analyses revealed @entity1 chromosomes with a modal number of 95 and 85, respectively. These cell lines have been passaged for over 2 years and are essentially immortal. The cells grew without contact inhibition as monolayers in liquid culture or as clones in soft agar. Electron microscopy revealed @entity320 cells containing premelanosomes and cultures free from contamination by fibroblasts. To categorize the morphologies of these cultured cells better by the Callender classification, they were grown as xenografts in the anterior chambers of @entity95 immunosuppressed with daily i.m. doses of @entity205 (10 mg/kg). @entity5 plaques were detected after 10 days. The eyes were enucleated and fixed in @entity406 and stained with @entity1710 and @entity1711 for histopathological evaluation. The xenograft from @entity27521 was found to consist predominantly of spindle B-type @entity320 cells. In contrast, the xenograft from @entity27521 contained epithelioid, spindle B and clear cell ("balloon") @entity320 cells. The ability of these cell lines to grow as xenografts confirmed their neoplastic origin. In fact, the types of the @entity2184 cells in the xenografts resembled those in the original biopsied @entity5 . This suggests that the morphology of @entity1 @entity2184 cells is an inherited trait and may be genetically fairly stable.
