Differentiation of healthy and malignant tissue in colon cancer XXXX using optical spectroscopy: A tool for image-guided surgery.

@entity5 @entity1 @entity14
BACKGROUND: Surgery for @entity14 aims for complete @entity5 resection. Optical-based techniques can identify @entity5 and surrounding tissue through the tissue specific optical properties, absorption and scattering, which are both influenced by the biochemical and morphological composition of the tissue. OBJECTIVE: To evaluate the feasibility of dual-modality Diffuse Reflectance Spectroscopy-Fluorescence Spectroscopy (DRS-FS) for discrimination between healthy and malignant tissue in colorectal surgery. METHODS: Surgical specimens from @entity14 @entity1 were measured immediately after resection using a fiber-optic needle capable of dual-modality DRS-FS. Model-based analyses were used to derive scattering and absorption coefficients and intrinsic fluorescence. Volume fractions of chromophores were estimated. Furthermore, optical data were recorded along a trajectory from healthy tissue towards @entity5 . RESULTS: Spectral characteristics were identified in 1,273 measured spectra from 21 specimens. Combined DRS and FS discriminated @entity5 from surrounding tissue with a sensitivity of 95% and a specificity of 88%. Significant spectral changes were seen along the trajectory from healthy tissue to @entity5 . CONCLUSION: This study demonstrates that dual-modality DRS-FS can identify @entity14 from surrounding healthy tissue. The quantification of comprehensible parameters allows robust classification and facilitates extrapolation towards the clinical setting. The technique, here demonstrated in a needle like probe, can be incorporated into surgical tools for optically guided surgery in the near future. Lasers Surg. Med. 9999:XX-XX, 2015.    2015 Wiley Periodicals, Inc.
