A novel repressor-type homeobox gene, ved, is involved in @entity58295 / XXXX -mediated dorsal organizer formation in @entity3392 .

@entity72217 @entity58295 @entity2879 @entity47771 @entity3392
@entity58295 (Dha/ @entity58295 ) is a homeodomain protein containing an Engrailed homology (Eh) 1 repressor motif. It is important in @entity3392 dorsal organizer formation. Dha/ @entity58295 interacted with a co-repressor Groucho through the Eh1 motif. Expression of a Dha/ @entity58295 fused to the transcriptional activator @entity2879 repressed dorsal axis formation and the expression of organizer genes but led to the dorsal expansion of expression of the homeobox gene vox/ @entity72217 , indicating that Dha/ @entity58295 functions as a transcriptional repressor for dorsal axis formation. We also isolated a novel homeobox gene, ved, whose expression was negatively regulated by dha/ @entity58295 . ved's sequence and expression profile were similar to those of vox/ @entity72217 and @entity47771 / @entity47771 . Like Vox/ @entity72217 and @entity47771 / @entity47771 , Ved acted as a transcriptional repressor. The combined inhibition of ved, vox/ @entity72217 , and @entity47771 / @entity47771 , by antisense morpholino injection, strongly dorsalized the embryos and elicited ventral expansion of organizer gene expression, compared with the effect of inhibiting each of these genes alone. These results suggest that ved is a target for the repressor Dha/ @entity58295 . Ved functions redundantly with vox/ @entity72217 and @entity47771 / @entity47771 to restrict the organizer domain.
