In Vitro Evaluation of XXXX Activity Against @entity5125 Biofilm Formation.

@entity5618 @entity5125 @entity1358 @entity20201 @entity880 @entity1098
UNASSIGNED: The present study aimed to assess the activity of @entity1358 ( @entity1358 ) containing bacteriocins on the formation and maintenance of biofilms developed by @entity5125 , and the associated effect of @entity5618 and @entity1098 ( @entity1098 ) on the formed biofilm. @entity1358 from 9 @entity880 bacteria (LAB) strains was tested for inhibitory activity against 85 @entity5125 isolates and 21 LAB strains. Then, 12 @entity5125 strains were selected based on genetic profiles and sensitivity to @entity1358 and were subjected to an in vitro assay to assess biofilm formation in microtiter plates, considering different culture media and incubation conditions. Based on these results, 6 @entity5125 strains were subjected to the same in vitro procedure to assess biofilm formation, being co-inoculated with @entity1358 . In addition, these strains were subjected to the same in vitro procedure, modified by adding the @entity1358 after biofilm formation. Relevant decrease in biofilm formation was observed in the first experiment, but @entity1358 added after biofilm formation did not eliminate them. @entity1358 from @entity20201 ET31 were selected due to its anti-biofilm activity, being associated to @entity1098 at different concentrations and tested for biofilm control of three strains of @entity5125 , using the same in vitro procedure described previously. Concentrated @entity5618 presented poor performance in eliminating formed biofilms, and @entity1098 concentration presented no evident interference on biofilm elimination. Twelve selected @entity5125 strains were positive for investigated virulence makers and negative for luxS gene, recognized as being involved in biofilm formation. Selected @entity5125 strains were able to produce biofilms under different conditions. CFSs have the potential to prevent biofilm formation, but they were not able to destroy already formed biofilms. Nevertheless, low concentrations of @entity1358 combined with @entity1098 caused a relevant reduction in already formed biofilms, but this association was not able to eliminate them. The activity of selected @entity1358 was demonstrated against @entity5125 -formed biofilms, being more effective when associated to @entity1098 at different concentrations.
