Photoprotective effects of two natural products on ultraviolet B-induced oxidative stress and apoptosis in SKH-1 XXXX skin.

@entity1 @entity5 @entity1489 @entity19 @entity13788 @entity3089 @entity3090 @entity2041 @entity36020 @entity2197 @entity1234 @entity1391 @entity842 @entity185 @entity6248
Solar ultraviolet radiation (UV) is the major cause of @entity1489 in @entity1 . Photochemoprevention with natural products represents a simple but very effective strategy for the management of cutaneous @entity5 . We studied the photoprotective activity of @entity6248 and red grape seed ( @entity13788 , Burgund Mare variety [BM]) extracts in vivo in an SKH-1 hairless @entity19 skin model. Fifty 8-week-old female SKH-1 hairless @entity19 were randomly divided into 5 groups (n   =   10 each): controls, UVB-irradiated, @entity36020 plus UVB-irradiated, BM plus UVB-irradiated, and @entity3089 ( @entity3090 ) plus UVB-irradiated. A dose of 4 mg/ @entity19 per cm   of skin area for both extracts was topically applied to the @entity19 30 minutes before a single-dose (240 mJ/cm  ) UVB exposure. @entity3090 dissolved in @entity1391 -buffered saline (pH 6.6; 0.067 M) was administered at 2 mg/ @entity19 per cm  . @entity2041 peroxidase and catalase activities, @entity2041 ( @entity2197 ), @entity1234 , @entity842 , and @entity185 activity were determined in skin homogenates 24 hours after irradiation. A single dose of UVB increased @entity2197 levels and @entity2041 peroxidase activity in the exposed skin. @entity36020 and BM pretreatment significantly decreased @entity2197 formation and @entity2041 peroxidase activity (P   <   .001) and inhibited UVB-induced lipid peroxidation (P   <   .0001) and @entity842 production ( @entity36020 : P   <   .06). Topical treatments with @entity36020 and particularly BM extracts (P   <   .002) significantly reduced @entity185 activity, indicating that the cells were protected against apoptosis. These results suggest that @entity36020 and BM extracts might be chemopreventive candidates for reducing UV-induced risk for @entity1489 .
