Lack of galectin-3 improves the functional outcome and tissue sparing by modulating inflammatory response after a compressive XXXX .

@entity19 @entity1735 @entity9675 @entity1566 @entity3804 @entity130
UNASSIGNED: @entity1566 ( @entity1566 ) is a traumatic event that results in motor, sensitive or autonomic function disturbances, which have direct impact on the life quality of the affected individual. Recent studies have shown that attenuation of the inflammatory response after @entity1566 plays a key role in the reestablishment of motor function. Galectin-3 is a pleiotropic molecule belonging to the @entity1735 -ligand lectin family, which is expressed by different cells in different tissues. Studies have shown that galectin-3 induces the recruitment and activation of neutrophils, monocytes/macrophages, lymphocytes and microglia. Thus, the aim of this study was to evaluate the effects of the lack of galectin-3 on the functional outcome, cellular recruitment and morphological changes in tissue, after @entity1566 . C57BL/6wild-type and galectin-3 knockout @entity19 were used in this study. A vascular clip was used for 1min to generate a compressive @entity1566 . By BMS we detected that the @entity9675 (-/-) presented a better functional outcome during the studied period. This finding is related to a decrease in the @entity130 and a higher volume of spared white matter at 7 and 42days post injury (dpi). Moreover, @entity9675 (-/-) @entity19 showed a higher number of spared fibers at 28dpi. Because of the importance of the inflammatory response after @entity1566 and the role that galectin-3 plays in it, we investigated possible differences in the inflammatory response between the analyzed groups. No differences in neutrophils were observed 24h after injury. However, at 3dpi, the @entity9675 (-/-) @entity19 showed more neutrophils infiltrated into the spinal tissue when compared with the WT @entity19 . At this same time point, no differences in the percentage of the @entity3804 /Arginase1 positive cells were observed. Remarkably, @entity9675 (-/-) @entity19 displayed a decrease in @entity3804 staining at 7dpi, compared with the WT @entity19 . At the same time, @entity9675 (-/-) @entity19 presented a more prominent Arginase1 stained area, suggesting an anti-inflammatory cell phenotype. Taken together, these results demonstrated that the lack of galectin-3 plays a key role in the inflammatory process triggered by @entity1566 , leading to better and early recovery of locomotor function.
