Synergistic Effect of Simple Sugars and XXXX Cellulose on the Production of a Cellulolytic Cocktail from Bacillus sp. AR03 and Enzyme Activity Characterization.

@entity7967 @entity3281 @entity1098 @entity3158 @entity969 @entity5070 @entity9266 @entity3363 @entity4202
UNASSIGNED: A cellulase-producing bacterium isolated from pulp and paper feedstock, Bacillus sp. AR03, was evaluated by means of a factorial design showing that peptone and @entity3363 were the main variables affecting enzyme production. Simple @entity3363 , individually and combined with @entity5070 cellulose (CMC), were further examined for their influence on cellulase production by strain AR03. Most of the mono and @entity7967 assayed presented a synergistic effect with CMC. As a result, a peptone-based broth supplemented with 10  g/L @entity969 and 10  g/L CMC maximized enzyme production after 96  h of cultivation. This medium was used to produce endoglucanases in a 1-L stirred tank reactor in batch mode at 30    C, which reduced the fermentation period to 48  h and reaching 3.12        0.02  IU/mL of enzyme activity. Bacillus sp. AR03 endoglucanases showed an optimum temperature of 60    C and a pH of 6.0 with a wide range of pH stability. Furthermore, presence of 10  mM @entity3281 and 5  mM @entity3158 produced an increase of enzyme activity (246.7 and 183.7  %, respectively), and remarkable tolerance to @entity4202 , @entity9266 , and @entity1098 was also observed. According to our results, the properties of the cellulolytic cocktail from Bacillus sp. AR03 offer promising features in view of potential biorefinery applications.
