Functional excitatory GABAA receptors precede ionotropic XXXX receptors in radial glia-like neural stem cells.

@entity11588 @entity2227 @entity1246 @entity2374 @entity11587 @entity1093 @entity50760 @entity143 @entity140
The involvement of neurotransmission in neuronal development is a generally accepted concept. Nevertheless, the precise regulation of neurotransmitter receptor expression is still unclear. To investigate the expression profiles of the most important ionotropic neurotransmitter receptors, namely @entity1246 (A) receptors ( @entity1246 (A)Rs), @entity143 receptors (NMDARs), and @entity140 receptors (AMPARs), quantitative RT-PCR, immunoblot analysis and patch clamp studies were performed in in vitro-generated neural stem cells (NSCs). This clearly defined cell line is closely related to radial glia cells, the stem cells in the neonate brain. We found functional @entity1246 (A)Rs of the subunit composition alpha2, @entity1093 , and gamma1 to be expressed. Unexpectedly, functional ionotropic @entity2227 receptors were absent. However, NSCs expressed the NMDAR subunits @entity11588 and @entity50760 , and the AMPAR subunit @entity11587 at the protein level, and @entity2374 at the mRNA level. The overexpression of functional NMDARs in NSCs led to an increased mRNA level of AMPAR subunits, indicating a role in synaptogenesis. Early neuronal markers remained unchanged. These data extend our knowledge about ionotropic neurotransmitter receptor expression during neuronal development and will aid further investigations on activity-dependent neurogenesis.
