@entity3204 : protective immunity against experimental XXXX induced by a DNA vaccine encoding the perforin-like protein 1.

@entity5517 @entity6026 @entity2047 @entity19 @entity27774 @entity3252 @entity3204
@entity3204 is an important zoonotic parasite infecting about one third of the world population, causing @entity5517 . @entity3204 perforin-like protein 1 (TgPLP1) is believed to be involved in the acute virulence of @entity3204 in @entity19 , and is therefore of interest as a vaccine candidate. In this study, we constructed a DNA vaccine expressing TgPLP1, and evaluated the immune response in Kunming @entity19 . The gene sequence encoding TgPLP1 was inserted into the eukaryotic expression vector pVAX I, and Kunming @entity19 were immunized intramuscularly with the plasmid. After immunization, we evaluated the immune response using lymphoproliferative assay, cytokine and antibody measurements, and the survival times of @entity19 challenged lethally with 1 10(3) tachyzoites of the virulent @entity27774 . The results showed that pVAX/TgPLP1 alone or with pVAX/ @entity6026 developed specific anti-TLA (T. gondii lysate antigen) antibodies and specific lymphocyte proliferative responses. Co-injection of pVAX/ @entity6026 significantly increased the production of IFN-y and @entity2047 . Further, challenge experiments showed that co-immunization of pVAX/TgPLP1 with pVAX/ @entity6026 significantly (P<0.05) increased survival time (12.7  1.2days) of immunized @entity19 , compared with pVAX/TgPLP1 alone (11.3  0.9days). These results demonstrate that TgPLP1 is a potential vaccine candidate against @entity3252 , worth further evaluation in other animal hosts. @entity6026 could enhance the immune effect of TgPLP1, prolonging the survival time of immunized @entity19 .
