Computer-assisted analysis of biopsy specimen microvessels predicts the outcome of XXXX treated with chemoradiotherapy.

@entity1 @entity5 @entity2279 @entity418 @entity7443
PURPOSE: A computer-assisted microvessel analysis system was developed to evaluate correlations between the architecture of biopsy specimen microvessels and the outcome for @entity1 with @entity418 treated with chemoradiotherapy. EXPERIMENTAL DESIGN: Biopsy specimens from 51 @entity1 with @entity418 (T(2-3), any N, M0) treated with chemoradiotherapy were immunostained with an anti- @entity2279 antibody and quantified using computerized image analysis. We evaluated the association of several microvessel factors with overall survival, including the ratio of total microvessel perimeter to total @entity5 area (TP/TA), the @entity5 hypoxic ratio, and the ratio of total microvessel number to total @entity5 area ( @entity7443 /TA). Results from traditional manual microvessel density (MVD) hotspot count and computerized hotspot count were compared and the relation between hotspot MVD count and survival rate was evaluated. RESULTS: The median follow-up time was 32 months. Both univariate and multivariate analyses revealed that computer-counted hotspot MVD and @entity7443 /TA and TP/TA ratios correlated significantly with the outcome of chemoradiotherapy. Kaplan-Meier survival curves showed that @entity1 with high computer-counted hotspot MVDs and high @entity7443 /TA and TP/TA ratios had better overall survival rate than @entity1 with low MVDs or ratios (P = 0.025, 0.008, and 0.031, respectively). Combining computer-counted MVD or @entity7443 /TA ratio with TP/TA ratio proved more predictive than any single factor. Two researcher-counted hotspot MVDs had no significant relation with outcome. CONCLUSION: Computer-assisted @entity5 microvessel analysis is a powerful tool in predicting the outcome for @entity1 with @entity418 treated with chemoradiotherapy because intraobserver and interobserver variability is minimized.
