Deletion of 13q14 remains an independent adverse prognostic variable in XXXX despite its frequent detection by interphase fluorescence in situ hybridization.

@entity1 @entity1603 @entity5 @entity2262 @entity1582 @entity995
Interphase fluorescence in situ hybridization (FISH) studies of chromosomal region 13q14 were performed to investigate the incidence and clinical importance of deletions in @entity1582 ( @entity1582 ). Monoallelic deletions of the @entity995 -1 (rb-1) gene and the D13S319 locus were observed in 48 of 104 @entity1 (46.2%) and in 28 of 72 (38.9%) @entity1 , respectively, with newly diagnosed @entity1582 . FISH studies found that 13q14 was deleted in all 17 @entity1 with karyotypic evidence of monosomy 13 or deletion of 13q but also in 9 of 19 @entity1 with apparently normal karyotypes. @entity1 with a 13q14 deletion were more likely to have @entity5 (P =.022), higher serum levels of @entity2262 (P =.059), and a higher percentage of bone marrow plasma cells (P =.085) than @entity1 with a normal 13q14 status on FISH analysis. In @entity1 with a deletion of 13q14, @entity1582 cell proliferation (Ki-67) was markedly increased (22.0% +/- 6.9% compared with 15.6% +/- 8.2% in @entity1 without the deletion; P =.0008). Evaluation of @entity1603 incorporation in 5 @entity1 revealed that both rb-1-deleted and rb-1-normal @entity1582 subpopulations were proliferative. The presence of a 13q14 deletion on FISH analysis was associated with a significantly lower rate of response to conventional-dose chemotherapy (40.8% compared with 78. 6%; P =.009) and a shorter overall survival (24.2 months compared with > 60 months; P <.005) than in @entity1 without the deletion. Multivariate analysis of prognostic factors confirmed the independent predictive value of 13q14 deletions for shortened survival. In conclusion, deletions of 13q14 are frequently detected by interphase FISH in @entity1 with newly diagnosed @entity1582 , correlate with increased proliferative activity, and represent an independent adverse prognostic feature in @entity1582 . (Blood. 2000;95:1925-1930)
