Autologous bone marrow transplantation in XXXX --preclinical immunologic studies.

@entity1 @entity10730 @entity981 @entity44693 @entity11511 @entity95 @entity1778 @entity54182 @entity4587
Immunologic aspects of autologous bone marrow transplantation (ABMT), immunodiagnosis, @entity1 monitoring, and the purging of bone marrow have been studied in individual @entity1 . It was demonstrated that the most sensitive method for detecting lymphoid cells which show the phenotypes of ALLs of B or T lineage was double immunofluorescence staining for nuclear @entity10730 ( @entity10730 ) and B or T lineage antigens. With the help of these sensitive tests in the presence of @entity95 complement (C'), MAbs CD10 (RFAL3 of IgM class), @entity54182 (SB4 of IgM class), and their cocktail were capable of eliminating greater than 3 log blast cells of B lineage @entity981 in 84%, 75.5%, and 90% of cases, respectively. The same reagents lysed 26.8%, 0%, and 45% of blasts in the presence of @entity1 C'. @entity4587 ( @entity44693 , IgG2) eliminated greater than 3 log @entity11511 blast cells in 73% of cases. The proliferative fractions of @entity1778 were also @entity10730 + and sensitive to lysis with MAb and C'. On the basis of these observations MABs were selected for purging in 36 @entity1 undergoing ABMT in first remission (10 @entity1 considered to be at a high risk of relapse), second and third remissions (23 and 2 @entity1 ), and without entering into remission (1 @entity1 ). The efficacy of eliminating the MAb-reactive cells from the bone marrow inoculum was also documented in five @entity1 . By the use of sensitive immunologic assay ( @entity10730 /cytoplasmic CD3 double staining) in @entity1 with @entity11511 , no residual @entity1778 (less than 10(-4] could be detected at the time of transplantation. Following an observation period of 5-34 months, 24 of the 36 @entity1 are alive and well with no procedure-related mortality.
