Evaluating the inhibitory potential of XXXX and inflammation enzymes: An in vitro and in silico study.

@entity1 @entity32 @entity2574 @entity12900 @entity42430 @entity1423 @entity1426 @entity534 @entity32766 @entity1234 @entity815 @entity1068 @entity1865
UNASSIGNED: Context Withania somnifera (L.) Dunal is traditionally used for treating various ailments, but lacks scientific evaluation. Objective This study evaluates Withania somnifera (WS) for its effect on platelet activity and inflammatory enzymes. Materials and methods Aqueous and ethanolic (1:1) leaf extracts were subjected to in vitro indirect haemolytic activity using @entity32766 venom, @entity1 @entity1423 was quantified for lipid peroxidation using @entity1426 (AA) as agonist and 5-lipoxygenase (5-LOX) levels were determined using standard spectrometric assays. Further, molecular docking was performed by the ligand fit method using molegro software package (Molegro ApS, Aarhus, Denmark). Results The study found that aqueous and @entity1068 extracts have very negligible effect (15%) with an IC50 value of 13.8   mg/mL on PLA2 from @entity32766 venom. Further, extracts of WS also had very little effect (18%) with an IC50 value of 16.6   mg/mL on @entity1234 ( @entity1865 ) formation. However, a 65% inhibition of 5-LOX with an IC50 value of 0.92   mg/mL was observed in 1:1 @entity1068 extracts. The same was evident from SAR model with the active ingredient @entity12900 binding predominantly on @entity815 77, Tyr 98, @entity534 99, @entity2574 164, Leu 168, Ser 382, @entity534 395, Tyr 396 and Tyr 614 with an atomic contact energy value of   -128.96 compared to standard @entity42430 (-103.61). Thus, the current study validates the application of WS for @entity32 . Conclusion This study reveals the inhibitory potential of W. somnifera on inflammatory enzymes and @entity1423 . Thus, WS can serve as a newer, safer and affordable medicine for @entity32 .
