Development of a method for the determination of XXXX in plasma by HPLC with fluorescence detection.

@entity29886 @entity3636 @entity1626 @entity1391 @entity21443 @entity12303
A simple and sensitive HPLC method has been developed for the determination of @entity29886 ( @entity21443 ) in plasma. Sample preparations were carried out by adding @entity1391 buffer (pH 7.4, 0.1 M), followed by extraction with @entity3636 . @entity21443 and the internal standard, @entity12303 (SAR), were separated on a reversed-phase column, and eluted with aqueous solution- @entity1626 (80:20 v/v). The fluorescence of the column effluent was monitored at lambda(ex) = 338 and lambda(em) = 425 nm. The retention times were 2.80 and 4. 40 min for @entity21443 and SAR, respectively. The method was shown to be linear from 1 to 1500 ng/mL (r(2) = 0.999). The detection and quantitation limit were 1 and 5 ng/mL, respectively. Mean recovery was determined as 80% by the analysis of plasma standards containing 150, 750 and 1500 ng/mL. Inter- and intra-assay precisions were 4.0% and 2.4%, respectively.
