Subtotal but not unilateral nephrectomy induces XXXX and protooncogene expression.

@entity4569 @entity1184 @entity1373 @entity13719 @entity5019 @entity35 @entity3803 @entity672
It is generally accepted that renal compensatory growth after unilateral nephrectomy (Uni) is due to prominent @entity1184 with no involvement of protooncogenes. Neither the balance between @entity1184 and @entity1373 nor the expression of the early-growth-related genes has been studied after subtotal nephrectomy (Nx). The occurrence of cystic tubular dilatations after Nx may suggest an excessive cell proliferation in this model. We measured DNA, RNA, and protein content, number of nuclei per tubular section, as well as @entity5019 , c-jun, @entity4569 , @entity3803 , @entity672 , and @entity13719 protooncogene expression in kidneys taken at time of surgery and 2, 7, and 14 days after sham operation (control @entity35 ), Uni, or Nx. After Uni, @entity1373 was greater than expected (+79% for DNA at day 14) and was associated with moderate @entity1184 (+11% for protein/DNA ratio). After Nx, compensatory growth was due only to @entity1373 (+117% for DNA at day 14), with unchanged protein/DNA ratio (vs. Uni, P < 0.02). The greater @entity1373 after Nx was confirmed by nuclei counting. The protooncogene mRNA expression was constantly absent in control and Uni @entity35 , whereas that of @entity5019 and c-jun genes was detected in Nx @entity35 at day 14 with a 2- to 12-fold increment. The @entity5019 and c-jun protein levels were also increased at that time in Nx @entity35 . This suggests the following: 1) the cellular events following Uni and Nx are not the same, and 2) the late protooncogene expression in Nx exclusively could favor a particular type of cell proliferation possibly more related with cystic formation than with actual compensatory growth.
