XXXX -responsive osteoblast nuclear matrix architectural transcription factor binds to the @entity35 type I collagen promoter.

@entity70598 @entity25256 @entity421 @entity35 @entity4727
In connective tissue, cell structure contributes to type I collagen expression. Differences in osteoblast microarchitecture may account for the two distinct cis elements regulating basal expression, in vivo and in vitro, of the @entity35 type I collagen alpha1(I) polypeptide chain ( @entity25256 ). The @entity25256 promoter conformation may be the penultimate culmination of osteoblast structure. Architectural transcription factors bind to the minor groove of AT-rich DNA and bend it, altering interactions between other trans-acting proteins. Similarly, nuclear matrix (NM) proteins bind to the minor groove of AT-rich matrix-attachment regions, regulating transcription by altering DNA structure. We propose that osteoblast NM architectural transcription factors link cell structure to promoter geometry and @entity25256 transcription. Our objective was to identify potential osteoblast NM architectural transcription factors near the in vitro and in vivo regulatory regions of the @entity35 @entity25256 promoter. Nuclear protein-promoter interactions were analyzed by gel shift analysis and related techniques. NM extracts were derived from @entity35 @entity421 cells and from @entity35 bone. The NM protein, @entity70598 , and a soluble nuclear protein, NP, both bound to two homologous poly(dT) elements within the @entity25256 in vitro regulatory region and proximal to the in vivo regulatory element. These proteins bound within the minor groove and bent the DNA. @entity4727 increased NP/ @entity70598 binding to both poly(dT) elements and decreased @entity25256 mRNA in the @entity421 cells. NP/ @entity70598 - @entity25256 promoter interactions may represent a molecular pathway by which osteoblast structure is coupled to @entity25256 expression.
