CD8+ T cells produce XXXX during acute rejection of @entity19 allogeneic skin grafts.

@entity32 @entity19 @entity638 @entity1961 @entity9298
BACKGROUND: Based on their chemoattractant properties, it is likely that chemokines play a role in recruiting alloantigen-primed T cells to allografts and in amplifying @entity32 within the graft. The graft-infiltrating leukocytes producing specific chemokines remain largely unknown. METHODS: We tested the intragraft RNA expression of the chemokine @entity638 (regulated on activation normal T expressed and secreted) and @entity9298 during rejection of full thickness, allogeneic skin grafts by C57BL/6 @entity19 . Grafts with different immunogenetic disparities were chosen to test expression when rejection was mediated by @entity1961 +, CD8+, or both @entity1961 + and CD8+ T cells. RNA expression was also tested in purified @entity1961 + and CD8+ T cell populations from skin graft recipients. Immunohistology was performed on graft sections to test colocalization of @entity638 protein and graft-infiltrating @entity1961 + and CD8+ T cells. RESULTS: Intra-allograft @entity638 RNA expression was not observed during @entity1961 + T cell-mediated rejection. Expression of @entity638 and @entity9298 RNA was observed at low levels in purified populations of CD8+, but not @entity1961 +, T cells from the spleen and lymph nodes of graft recipients beginning at day 7 after transplantation and increased thereafter. Intra-allograft @entity638 protein was associated with a small number of graft-infiltrating CD8+ T cells but was also associated with endothelial cells and with many graft-infiltrating @entity1961 + T cells. CONCLUSIONS: CD8+ T cells produce @entity638 during allogeneic skin graft rejection. In the allograft, the chemokine also colocalizes with @entity1961 + T cells that do not produce @entity638 .
