Rapid intranasal delivery of XXXX acetyltransferase in the active form to different brain regions as a model for enzyme therapy in the CNS.

@entity16 @entity6929 @entity14079 @entity35 @entity2755 @entity2359
BACKGROUND: The blood brain barrier (BBB) is critical for maintaining central nervous system (CNS) homeostasis by restricting entry of potentially toxic substances. However, the BBB is a major obstacle in the treatment of @entity2359 and @entity16 due to the restrictive nature of the barrier to many medications. Intranasal delivery of active enzymes to the brain has therapeutic potential for the treatment of numerous CNS @entity14079 caused by chemical threat agents. NEW METHOD: The aim of this work is to provide a sensitive model system for analyzing the rapid delivery of active enzymes into various regions of the brain with therapeutic bioavailability. RESULTS: We tested intranasal delivery of @entity2755 acetyltransferase (CAT), a relatively large (75kD) enzyme, in its active form into different regions of the brain. CAT was delivered intranasally to anaesthetized @entity35 and enzyme activity was measured in different regions using a highly specific High Performance Thin Layer Chromatography (HP-TLC)-radiometry coupled assay. Active enzyme reached all examined areas of the brain within 15min (the earliest time point tested). In addition, the yield of enzyme activity in the brain was almost doubled in the brains of @entity35 pre-treated with @entity6929 ( @entity6929 ). COMPARISON WITH EXISTING METHOD (S): Intranasal administration of active enzymes in conjunction with @entity6929 to the CNS is both rapid and effective. CONCLUSION: The present results suggest that intranasal enzyme therapy is a promising method for counteracting CNS chemical threat poisoning, as well as for treating @entity14079 .
