Immunolabeling of the inhibin-bA and -bB subunit in normal and malignant @entity1 cervical tissue and XXXX cell lines.

@entity1 @entity8703 @entity5 @entity189 @entity105 @entity3146
OBJECTIVES: Inhibins, dimeric peptide hormones composed of an a-subunit and 1 of 2 possible b subunits (bA or bB), exhibit substantial roles in @entity1 reproduction and in @entity8703 . However, it is still unclear whether normal and cancerous cervical tissues as well as @entity5 cell lines express the inhibin-bA and -bB subunits. MATERIALS AND METHODS: Normal @entity1 uterine cervical tissue was obtained from 4 premenopausal nonpregnant @entity1 . In addition, a total of 32 specimens of @entity3146 ( @entity3146 ) of different stages were obtained ( @entity3146 1 = 10, @entity3146 2 = 9, and @entity3146 3 = 13). Moreover, 30 @entity5 samples of well-differentiated (grade 1; n = 10), moderate differentiated (grade 2; n = 10), and poorly differentiated (grade 3; n = 10) grading were analyzed. RESULTS: An immunohistochemical staining reaction for inhibin-bA and -bB subunits could be observed in normal and malignant cervical tissue as well as in @entity5 cell lines. Regarding inhibin-bA significant differences were observed between normal tissue and @entity3146 1 and @entity3146 3. Moreover, the immunohistochemical staining reaction for inhibin-bA was significantly higher in @entity3146 3 compared with that in cervical @entity189 grades 1 and 2. The inhibin-bB expression was higher in @entity3146 and @entity5 compared with that in normal cervical tissue. Inhibin-bB was significantly higher in @entity3146 2 and @entity3146 3 compared with @entity5 tissues of histological grade 1. In addition, a significant increase of the staining intensity was observed between @entity5 grades 1 and 2 as well as grade 3. CONCLUSIONS: Both inhibin-b subunits demonstrated a differential expression in @entity3146 and @entity5 , suggesting important roles in cervical @entity105 . Inhibin-bA might be important during progression of @entity3146 , whereas the inhibin-bB subunit could exert a substantial function during differentiation of cervical @entity189 . Moreover, the synthesis of this subunit in cervical @entity189 cell lines also allows the use of this cell line to elucidates their functions in @entity5 pathogenesis.
