Does Thermosensitive Liposomal XXXX Improve End-Point Survival after Percutaneous Radiofrequency Ablation of @entity96 in a @entity19 Model?

@entity41 @entity96 @entity19 @entity5 @entity1206 @entity420
UNASSIGNED: Purpose To investigate the role of thermosensitive liposome-encapsulated @entity1206 (Thermo-Vin) in combined radiofrequency (RF) ablation of @entity96 . Materials and Methods Approval from the institutional animal care and use committee was obtained before this study. First, the anticancer efficacy of Thermo-Vin was assessed in vitro (H22 cells) for 72 hours at 37  C or 42  C. Next, 203 H22 liver @entity420 were implanted in 191 @entity19 for in vivo study. @entity5 were randomized into seven groups: (a) no treatment, (b) treatment with RF ablation alone, (c) treatment with RF ablation followed by free @entity1206 (Free-Vin) at 30 minutes, (d) treatment with RF ablation followed by empty liposomes (Empty-Lip+RF), (e) treatment with RF ablation followed by Thermo-Vin (5 mg/kg), (f) treatment with RF ablation followed by Thermo-Vin (10 mg/kg), and (g) treatment with RF ablation followed by Thermo-Vin (20 mg/kg). @entity5 destruction areas and pathologic changes were compared for different groups at 24 and 72 hours after treatment. Kaplan-Meier analysis was used to compare end-point survival ( @entity5 < 30 mm in diameter). Additionally, the effect of initial @entity5 size on long-term outcome was analyzed. Results In vitro, both Free-Vin and Thermo-Vin dramatically inhibited H22 cell viability at 24 hours. Likewise, in vivo, 10 mg/kg Thermo-Vin+RF ablation increased @entity5 destruction compared with RF ablation (P = .001). Intratumoral @entity1206 accumulation with Thermo-Vin+RF increased 15-fold compared with Free-Vin alone. Thermo-Vin substantially increased apoptosis at the coagulation margin and suppressed cellular proliferation in the @entity41 (P < .001). The Thermo-Vin+RF study arm also had better survival than the arm treated with RF ablation alone (mean, 37.6 days    20.1 vs 23.4 days    5.0; P = .001), the arm treated with Free-Vin+RF (23.3 days    1.2, P = .002), or the arm treated with Empty-Lip+RF (20.8 days    0.4, P < .001) in animals with medium-sized (10-12-mm) @entity5 . No significant difference in end-point survival was noted in the treatment arms with large or small @entity5 . Conclusion Thermo-Vin can effectively increase @entity5 destruction and improve animal survival. End-point survival is most affected in animals with medium-sized @entity5 , suggesting that combination therapy should be tailored to @entity5 size and the expected volume of ablation of the device used. (  ) RSNA, 2016 Online supplemental material is available for this article.
