Anti-Citrullinated Protein Antibodies Induce Macrophage Subset Disequilibrium in XXXX @entity1 .

@entity1 @entity571 @entity309 @entity20630 @entity7134 @entity22367
UNASSIGNED: We used samples from @entity309 ( @entity309 ) @entity1 to examine whether Anti-citrullinated protein antibodies (ACPAs) alter macrophage subset distribution and promote @entity309 development. Macrophage subset distributions and @entity20630 ( @entity20630 ) and @entity22367 expressions were analyzed. ACPAs were purified by affinity column. After @entity309 and @entity571 ( @entity571 ) @entity1 ' macrophages were cocultured with ACPAs, macrophage subsets and @entity20630 and @entity22367 expressions were measured. Small interfering RNAs (siRNAs) were transfected into @entity7134 -activated cells to suppress @entity20630 or @entity22367 . Fluorescence-activated cell sorting (FACS), Western blot, and immunohistochemistry were performed. Macrophage subset disequilibrium occurred in @entity309 @entity1 synovial fluids. @entity20630 and @entity22367 were all expressed in the synovial fluid and synovium. ACPAs (40  IU/ml) could induce macrophages to polarize to M1 subsets, and the percentage of increased M1/M2 ratio of @entity309 @entity1 was higher than that of the @entity571 @entity1 . ACPAs also induce @entity20630 and @entity22367 protein expressions. @entity22367 siRNA transfection impaired @entity7134 activity significantly. We demonstrated that macrophage subset disequilibrium occurred in @entity309 @entity1 . ACPAs induced @entity22367 activity and led to M1 macrophage polarization.
