Oogonial Precursor Cell-Derived Autologous Mitochondria Injection to Improve Outcomes in XXXX With Multiple IVF Failures Due to Low Oocyte Quality: A Clinical Translation.

@entity1 @entity52 @entity2383
BACKGROUND: @entity2383 has been suggested as a major cause of age-induced decline in oocyte quality. In the past, donor oocyte cytoplasmic transfer showed some success but was abandoned due to the concerns with heteroplasmy. Recent studies indicated presence of oogonial precursor cells (OPCs) in the @entity1 ovary, which could be an autologous source of "healthy mitochondria." We sought to investigate the clinical efficacy of OPC-derived @entity2383 ( @entity2383 ) to improve oocyte quality in @entity1 with multiple @entity52 ( @entity52 ) failures. METHODS: The OPCs were isolated from laparoscopically obtained ovarian cortical pieces by cell sorting using a monoclonal anti-vasa homolog (anti-DDX) antibody. They were then disrupted and mitochondria were isolated. Reconstituted mitochondria were injected into each oocyte during intracytoplasmic sperm injection. Paired comparisons were made between the first failed cycles and the post- @entity2383 cycles. RESULTS: Of the 15 @entity1 undergoing ovarian stimulation, 2 were canceled and 3 decided to pool oocytes for later @entity2383 . In remaining 10 (mean age 34.7    4.1), @entity2383 significantly improved fertilization rates (49.7    31.3 vs 78.3    18.9; P = .03) with a trend for better embryo grades (2.3    0.3 vs 3.1    0.7; P = .08). Four of 10 @entity1 conceived after single frozen embryo transfer and 3 after confirmation of diploidy via array comparative genomic hybridization (aCGH) (clinical pregnancy/embryo transfer = 4/10). CONCLUSION: These data show encouraging results for @entity2383 in comparison to previous failed @entity52 cycles.
