[Changes in bronchoalveolar lavage fluid of XXXX exposed to metal grinding dusts].

@entity6032 @entity880 @entity3674 @entity35 @entity3759
OBJECTIVE: To investigate the effects of composite grinding dusts on @entity35 respiratory system. METHODS: @entity35 were administrated with grinding dusts by intratracheal injection. After 2 weeks, the total numbers of cells, the percentage of differential cell, the survival rate of cell, and the activity of @entity880 dehydrogenase(LDH) and alkaline phosphatase( @entity6032 ) in bronchoalveolar lavage fluid were analyzed. RESULTS: Along with increasing concentration of grinding dusts, the total number of cells in lavage also increased, and was more than that in @entity3674 group. Compared with control group, the percentage of neutrophil in lavage of @entity35 treated with grinding dust and @entity3674 significantly increased and meanwhile that of macrophage significantly decreased[PMN: @entity3674 group (33.83 +/- 4.54)%; grinding dusts group (26.50 +/- 3.99)%, (36.00 +/- 3.58)%, (38.00 +/- 2.10)% at 10, 25, 50 mg/ml respectively. Macrophages: @entity3674 group (62.17 +/- 4.54)%; grinding dusts group (70.83 +/- 3.66)%, (60.83 +/- 2.14)%, (58.17 +/- 2.48)%] while those in control group were (2.83 +/- 0.75)%, (95.67 +/- 1.21)% respectively. The cell survival rate in lavage in control group was 80%, but that in grinding dust and @entity3759 group significantly decreased(P < 0.01). The activity of LDH and @entity6032 in all @entity35 treated with dusts obviously increased, and there was significant difference compared with control group(P < 0.01 or P < 0.05). Furthermore, there was significant difference between grinding dust group and @entity3674 group, and between grinding dust group and @entity3759 group respectively. CONCLUSION: Metal grinding dust is very harmful to @entity35 's lung cells and may cause fibrogenesis in the lungs.
