[Effects of XXXX on the apoptosis of PC-12 cells under the induction of b-amyloid peptide].

@entity1976 @entity30765 @entity19 @entity98 @entity71844
OBJECTIVE: To study the protective effects of @entity71844 ( @entity30765 ) on the Ab(25-35)-induced apoptosis in PC-12 cells. METHODS: The apoptosis of PC-12 cell was analyzed by @entity98 assay, transmission electron microscope and PI method at different concentrations of @entity30765 (0.1, 1.0, 10, 100   mol/L) with the addition of Ab or not. The expressions of @entity1976 and cytochrome C (Cyt-C) were detected by Western blot. RESULTS: As demonstrated by the @entity98 assay, the values of cell viability were 76.5%    1.1%, 84.2%    1.3%, 89.5%    1.3% and 81.9%    1.9% at various concentration (0.1, 1.0, 10, 100   mol/L) of @entity30765 respectively. The model group was 71.7%    1.4%. It was revealed that the former could significantly prevent the cell viability under the induction of Ab(25-35) (P < 0.05). A pretreatment with 10   mol/L @entity30765 could significantly inhibit the decrease of viability under the induction of Ab(25-35) (P < 0.05). PI showed that the apoptosis rate of the 10   mol/L @entity30765 treatment group was significantly lower than that of the model group. Under electron microscope, the characteristics of cell apoptosis were significant in the model group. And the cell morphology of the 10   mol/L @entity30765 treatment group was normal. The expression rate of @entity1976 protein in the 10   mol/L @entity30765 treatment group was obviously higher than that in the model group. @entity19 was weakly expressed in nerve cells of the normal and the 10   mol/L @entity30765 groups. But it had a strong expression in the model group. CONCLUSION: @entity30765 prevents the injury of PC12 cells by Ab. And the mechanism may be related to the elevated level of @entity1976 and the inhibited mitochondrial release of Cyt-C.
