Predictive assays for the therapy of XXXX .

@entity188 @entity1 @entity5
Cytofluorometric DNA measurements showed that about 55% of @entity188 (129 @entity1 ) had @entity5 with an abnormal DNA content (DNA aneuploidy). For @entity1 with such a @entity5 the prognosis was worse than for @entity1 with DNA diploid @entity5 . From the DNA histograms the number of S-phase cells was calculated. In @entity5 with the stage pT3, which disseminated to lymph nodes or metastasized, a higher number of S-phase cells was found than in @entity5 with the staging pT3N0M0. In all untreated @entity5 cells with micronuclei were found. This demonstrated cell loss. In most @entity5 this effect was considerable. The ratio:number of S-phase cells/number of cells with micronuclei may allow a rough estimate for cell turnover. In @entity1 with a bad prognosis and in those @entity1 who had a local recurrence after resection of the @entity5 this ratio was high. In 34 @entity1 the parameters were measured before and after preoperative radiotherapy. In some @entity5 a rapid increase of S-phase cells occurred after irradiation, this effect might express repopulation. In these @entity1 a local recurrence was frequently found. From the data obtained so far a prediction for local recurrences might be possible from the determination of nuclear protein bound SH-groups. The determination of micronuclei indicated that it can be used as a measure for radiation response in @entity5 . All parameters show a high variability between individual @entity5 . A further study is useful whether the measured parameters are suitable as predictors.
