Comparison of automated cellular imaging system and manual microscopy for immunohistochemically stained cryostat sections of XXXX specimens applying @entity876 , ki-67 and @entity8780 .

@entity1 @entity1149 @entity8780 @entity2 @entity876
Seventy-one frozen lung tissue specimens from 52 @entity1 suffering from @entity1149 ( @entity1149 ) were analysed in this study. Cryostat sections were stained with monoclonal antibodies against @entity876 , ki-67 and @entity8780 , all of which are of prognostic value in @entity1149 . Slides were evaluated by standard manual microscopy (MM) and using a new Automated Cellular Imaging System (ACIS). The results obtained with ACIS correlated significantly with MM examination (p<0.001). However, ACIS showed a higher sensitivity, especially for specimens with a low infiltration volume. In 15 (6.8%) MM negative cases singular positive cells were identified with ACIS. In cases with a high infiltration volume subjective (MM) quantitation tended to overestimate the number of infiltrating cells. ACIS guaranteed a high reproducibility of data. We conclude that ACIS-assisted analysis is a valid means of investigating the @entity876 , ki-67 and @entity8780 antibody expression in cryostat sections of @entity2 specimens. ACIS can complement conventional manual microscopy due to its higher accuracy, sensitivity and better reproducibility of data.
