Detection of @entity526 in beach water within 1 hour using immunomagnetic separation and XXXX bioluminescence.

@entity855 @entity852 @entity1172 @entity526
The contamination of beach waters occurs from the discharge of storm water and sanitary sewer overflows containing faecal material. Additional faecal material derives from discharge of animals and waterfowl. In order to protect public from exposure to faecal-contaminated water, it is required to test enteric indicators in beach water. The problem is that the traditional culture-based methods cannot meet this goal because it takes too long (>24 h), so the results are not available until a day later. A rapid method for testing beach water for @entity526 within 1 h has been developed. @entity1172 ( @entity1172 ) and @entity855 bioluminescence were used for selective capture and quantification, respectively. This rapid method was compared to the current method (m-TEC) using beach water samples. The beach samples were prefiltered with a 20 microm pore size filter in order to remove algae, plant debris and large particles. The results showed that the prefiltration step did not trap the bacteria which were present in the original water samples. The prefiltered water was then passed through a 0.45 microm pore size filter for concentration. The deposited bacteria were resuspended and then mixed with superparamagnetic @entity852 beads (diameter of 0.6 microm) that were coated with E. coli antibodies. After @entity1172 , the quantification of the @entity526 was done by @entity855 bioluminescence. The results obtained with @entity1172 - @entity855 bioluminescence correlated well with the plate count method (Rsq = 0.93). The detection limit of the assay was about 20 CFU/100 mL, which is well below the US EPA limits for recreational water. The entire procedure can be completed in less than 1 hour. The necessary equipment is portable and was tested on-site.
