Efficacy of autologous serum in XXXX adipose-derived stem cells; cell markers, growth factors and differentiation.

@entity1 @entity10793 @entity11968 @entity7 @entity2279 @entity1759 @entity2252 @entity2393 @entity2280 @entity4434 @entity2241 @entity8020
@entity1 adipose-derived stem cells (hASCs) are a feasible source of stem cells for use in clinical applications. hASCs are typically cultured in medium containing @entity2241 ( @entity4434 ); however, use of @entity4434 is not recommended due to issues of clinical safety with regard to infections or immune response. Replacement of @entity4434 with autologous human serum (autoHS) can eliminate these problems; however, their maintainability as potent ASCs in autoHS needs to be confirmed. Thus, we conducted an investigation of characterizations and functions of hASCs grown in medium containing autoHS compared to @entity4434 . Cell counting and the WST-8 assay were used in assessment of the proliferation rate. In hASC cultured with culture medium plus autoHS or @entity4434 , cell phenotypes were characterized by flow cytometry ( @entity10793 , @entity2393 , @entity2279 , @entity2280 , and @entity1759 ) and expression of @entity2252 , @entity11968 , IGF, @entity8020 , NGF, and @entity7 was determined by RT   PCR. Adipogenic differentiation was confirmed by oil red O stain. hASC showed greater expansion in AutoHS than in @entity4434 . Cell surface markers of hASCs grown in autoHS (autoHS-hASCs) were similar to markers of those grown in @entity4434 ( @entity4434 ). AutoHS-hASCs also expressed multiple growth factors as well as @entity4434 . In addition, autoHS was effective in growth of hASCs as well as @entity4434 and autoHS-hASCs retained their ability for adipogenic differentiation. In summary, autoHS-hASCs have multiple growth factor expressions with the same cell surface markers as @entity4434   hASCs in vitro. Our results suggest that autoHS can provide sufficient ex vivo expansion of hASCs.
