@entity8945 promoter methylation in primary XXXX .

@entity1 @entity5 @entity14 @entity3 @entity8945
@entity8945 gene, a transcription factor that belongs to the family of basic helix-loop-helix proteins, has been related to @entity5 progression and @entity3 in different @entity5 . The aim of our study was to investigate @entity8945 promoter methylation in @entity1 with primary @entity14 and determine its correlation with prognostic factors and disease outcome. Seventy-three @entity1 with primary @entity14 were studied. From each @entity1 two tissue samples were collected: one sample of the @entity5 and one sample of normal colorectal tissue from an area located 15  cm away from the @entity5 . Samples of colorectal mucosa obtained from 30 individuals without @entity5 were also studied as a control group. All tissues were analyzed through methylation-specific PCR. @entity8945 hypermethylation was detected in colorectal specimens of 46 @entity1 with @entity5 , but in none of the tissues from the nonmalignant control group (p   <   0.001). In @entity5 @entity1 , @entity8945 hypermethylation was found in 38 of 73 @entity5 samples as compared with 20 of 73 matched samples of non-cancerous colorectal tissue (P   =   0.001). @entity8945 hypermethylation was not correlated with prognostic predictors for the disease outcome, @entity1 ' overall survival and disease-free survival rates. We concluded that @entity8945 hypermethylation is present in the colon and rectum of most @entity1 with @entity14 , suggesting this molecular alteration may be involved in the process of @entity14 .
