Influence of synthetic superparamagnetic XXXX on dendritic cells.

@entity19 @entity8271 @entity1933 @entity11787 @entity68
BACKGROUND: This study investigated the influence of synthetic superparamagnetic @entity1933 (SPIO) on dendritic cells and provides a possible method for labeling these cells. METHODS: SPIO nanoparticles were prepared, and their morphology and magnetic properties were characterized. The particles were endocytosed by dendritic cells generated from @entity19 bone marrow. Labeling efficiency and cellular uptake were analyzed by Prussian blue staining and quantitative spectrophotometric assay. Meanwhile, the surface molecules, cellular apoptosis, and functional properties of the SPIO-labeled dendritic cells were explored by flow cytometry and the mixed lymphocyte reaction assay. RESULTS: The synthetic nanoparticles possessed a spherical shape and good superparamagnetic behavior. The mean concentration of @entity68 in immature and mature dendritic cells was 31.8    0.7  g and 35.6    1.0  g per 1   10(6) cells, respectively. After 12 hours of incubation with SPIO at a concentration of 25  g/mL, nearly all cells were shown to contain @entity68 . Interestingly, cellular apoptosis and surface expression of @entity8271 , @entity11787 , major histocompatibility II, and chemokine receptor 7 in mature dendritic cells were not affected to any significant extent by SPIO labeling. T cell activation was maintained at a low ratio of dendritic cells to T cells. CONCLUSION: SPIO nanoparticles have good superparamagnetic behavior, highly biocompatible characteristics, and are suitable for use in further study of the migratory behavior and biodistribution of dendritic cells in vivo.
