Ex-vivo fine needle aspiration. A new method of xenografting XXXX .

@entity4153 @entity39 @entity5 @entity19 @entity957 @entity1149 @entity2 @entity420
Ex-vivo needle aspiration (xvFNA) has been rarely used to obtain viable @entity5 cells. It has been occasionally employed for short-term cultures. @entity2 in athymic @entity19 provides a good animal model for the study of this @entity5 . Successful engraftment using conventional methods has been disappointing) low (d 40%). Enzymatic digestion of the @entity5 fragments to obtain cell suspension lowers viability. We postulated that xvFNA might provide readily available @entity5 cell suspensions for xenografting @entity2 and that it would provide a higher success rate of engraftment than the conventional techniques. We aseptically performed xnFNA in 35 cases of freshly resected @entity1149 . These included 15 @entity420 , 17 @entity957 and 3 undifferentiated @entity4153 (UNSCC). @entity5 cell suspensions were injected subcutaneously in athymic @entity19 . @entity5 @entity39 in the aspirates ranged from 20-90% (median 60%). Gross evidence of engraftment was seen in 30 of 35 cases (85.7%) 1-19 weeks postimplantation (median 2 weeks). This was seen in UNSCC (3/3), @entity957 (13/17) and @entity420 (14/15). Xenograft sizes ranged from 5-34 mm (median 19 mm). They showed similar morphology to the @entity5 . Ex-vivo FNA used for harvesting @entity2 cells and their xenografting is an effective method for obtaining viable material for studying this @entity5 .
