Induction of XXXX interleukin 1: stimuli and responsive primary cells.

@entity634 @entity19 @entity3019 @entity1359 @entity5003 @entity637 @entity3457
An @entity634 ( @entity634 ) cDNA probe and an @entity5003 responsive T-cell clone (D10.G4; half-maximal stimulation, 0.1-1 pM) have been used to study the production of @entity5003 by primary @entity19 cell populations, particularly macrophages and dendritic cells. Spleen and peritoneal macrophages produced @entity5003 mRNA and released biologically active @entity5003 when challenged with lipopolysaccharide (LPS). Induction of @entity5003 was evident over a dose range of 0.01-10 micrograms of LPS per ml, and maximal mRNA levels were maintained from 4 to 20 hr. Several other stimuli did not induce @entity5003 in cultured macrophages, including @entity1359 , @entity3019 , @entity637 , and activated T cells. @entity3457 cells could markedly reduce the response of peritoneal macrophages to LPS. When other cell types were compared with macrophages, keratinocytes had high levels of @entity5003 mRNA, apparently in response to endogenous LPS. However B and T lymphocytes did not yield detectable @entity5003 during proliferative responses to LPS and Con A, respectively, while dendritic cells produced little or no @entity5003 when challenged with a battery of stimuli. Therefore, @entity5003 may not be required for the potent accessory function of dendritic cells in lymphocyte mitogenesis. The results indicate that macrophages and dendritic cells have different secretory capacities. The macrophage is the principal leukocyte that synthesizes @entity5003 , and select stimuli increase and decrease the levels of macrophage @entity5003 mRNA.
