@entity1481 Protects Umbilical Cord Mesenchymal Stem Cells Against XXXX and Serum Deprivation Induced Apoptosis by Activation of @entity584 .

@entity1 @entity588 @entity4404 @entity584 @entity2306 @entity519 @entity1481 @entity2904 @entity583
UNASSIGNED: Background : Mesenchymal stem cell (MSC) transplantation is a promising therapy for cardiac repair. However, the efficacy is limited by the poor viability of MSCs in the infarcted heart. Recent findings have implicated that @entity1481 ( @entity2904 ) enhanced the survival of the stem cells under various conditions. However, as the stem cells in these studies were animal-derived, little information is available about the effects of @entity2904 on @entity1 MSCs. Herein, we propose that @entity2904 may protect @entity1 MSCs against apoptosis induced by @entity519 /Serum deprivation (H/SD). Methods: @entity1 umbilical cord MSCs (UC-MSCs) from Wharton's jelly were pretreated with 10  M @entity2904 of H/SD with or without the @entity584 inhibitor @entity2306 . The morphological changes were assessed using Hoechst 33342. Apoptosis was evaluated via @entity4404 /PI staining; and apoptosis-related proteins were detected using Western-blot. Protein chip technology was used to screen for differences between the cell supernatants. Results : @entity2904 had a significant protective effect against H/SD-induced apoptosis, accompanied by an increase in @entity588 and p- @entity584 . The @entity2904 -mediated anti-apoptotic effect on MSCs could be attenuated by treatment with @entity2306 . Moreover, protein chip assays showed that @entity2904 treatment increased the paracrine functions of MSCs. Conclusion : @entity1481 protects @entity1 UC-MSCs from H/SD-induced apoptosis via the @entity584 pathway and may therefore be a potentially useful therapeutic adjunct for transplanting MSCs into damaged heart after @entity583 .    2015 S. Karger AG, Basel.
