Elicitation of enteroluminal neutrophils by enterotoxigenic and nonenterotoxigenic strains of @entity526 in XXXX .

@entity1707 @entity17052 @entity526
In intact neonatal piglets, two strains of enterotoxigenic @entity526 ( @entity17052 ), which could adhere to epithelial cells and thus colonize the small intestine, attracted greater numbers of neutrophils into the lumen and wall of the intestine than did a nonenteropathogenic strain of @entity526 . Ligated loops of small intestine in 8-week-old @entity1707 were used in attempts to identify the attributes of @entity17052 involved in stimulating an increased enteroluminal migration of neutrophils. A nonenteropathogenic strain of @entity526 did not attract neutrophils into the intestinal lumen in this model. However, three of the five @entity17052 strains tested did so. The three positive strains all produced heat-stable enterotoxin type b (STb). Neither of the negative @entity17052 strains produced STb. An STb-containing culture supernatant prepared from a strain of @entity526 which contained an STb plasmid did not attract significantly more neutrophils than did a control supernatant prepared from the same strain of @entity526 without the plasmid. The @entity17052 strains which attracted neutrophils in loops did not associate intimately with loop villi more consistently, nor did they grow to higher numbers in loops than strains which did not. It was concluded that there are increased numbers of neutrophils in the intestinal lumen during @entity17052 infection of newborn @entity1707 . However, attempts to identify the attribute(s) of @entity17052 responsible for eliciting enteroluminal neutrophils were not successful.
