Increased expression of B cell activation factor supports the abnormal expansion of transitional B cells in XXXX .

@entity1 @entity22167 @entity8410 @entity7501 @entity9509 @entity1252 @entity29459 @entity13434 @entity4020 @entity73218
OBJECTIVE: To examine the relationship between @entity4020 ( @entity4020 ) and dysregulation of B cell activation factor ( @entity8410 ) and specific B cell phenotypes in @entity1252 ( @entity1252 ). METHODS: Four-color flow cytometry was used to examine the peripheral B cell populations in @entity1 with @entity1252 . RNA was isolated from the peripheral blood of 87 @entity1 and @entity8410 expression was determined by quantitative polymerase chain reaction (PCR) and normalized to @entity7501 . The expression levels of 5 IFN-responsive genes ( @entity73218 , @entity22167 , @entity9509 , @entity29459 , and @entity13434 ) were determined by quantitative PCR and totaled to generate a global IFN score. The correlations were examined between peripheral B cell populations (including transitional, pregerminal, plasmablasts, and memory) and the expression of @entity8410 and the global IFN score. RESULTS: Examination of the peripheral B cell populations in @entity1252 demonstrated a relative expansion of the transitional B cell and plasmablast compartment and a reduction in the memory B cell population. Expressions of @entity8410 and global IFN score were elevated in @entity1 with @entity1252 compared to healthy controls. A strong positive correlation was noted between @entity8410 expression and the relative proportion of late transitional (T2) B cells. The proportions of more mature B cell phenotypes did not correlate with @entity8410 expression. The global IFN score was strongly associated with the level of @entity8410 expression and moderately correlated with the proportion of late transitional B cells. CONCLUSION: The findings suggest that elevated @entity8410 expression supports expansion of the T2 B cell compartment and contributes to a breach in tolerance in @entity1 with @entity1252 .
