High-resolution analysis of genomic copy number alterations in XXXX by microarray-based comparative genomic hybridization.

@entity24328 @entity150 @entity8625 @entity5
We have screened 22 @entity150 -derived cell lines and one normal urothelium-derived cell line for genome-wide copy number changes using array comparative genomic hybridization (CGH). Comparison of @entity8625 with existing multiplex-fluorescence in situ hybridization ( @entity24328 ) results revealed excellent concordance. Regions of gain and loss were defined more accurately by @entity8625 , and several small regions of deletion were detected that were not identified by @entity24328 . Numerous genetic changes were identified, many of which were compatible with previous results from conventional CGH and loss of heterozygosity analyses on @entity150 . The most frequent changes involved complete or partial loss of 4q (83%) and gain of 20q (78%). Other frequent losses were of 18q (65%), 8p (65%), 2q (61%), 6q (61%), 3p (56%), 13q (56%), 4p (52%), 6p (52%), 10p (52%), 10q (52%) and 5p (43%). We have refined the localization of a region of deletion at 8p21.2-p21.3 to an interval of approximately 1 Mb. Five homozygous deletions of @entity5 suppressor genes were confirmed, and several potentially novel homozygous deletions were identified. In all, 15 high-level amplifications were detected, with a previously reported amplification at 6p22.3 being the most frequent. Real-time PCR analysis revealed a novel candidate gene with consistent overexpression in all cell lines with the 6p22.3 amplicon.
