Apoptotic cells in the XXXX endometrium and placental villi: pitfalls in applying the TUNEL method.

@entity10730 @entity1 @entity5852 @entity548 @entity39 @entity63 @entity406 @entity1977 @entity5617 @entity982 @entity1188 @entity420 @entity528
Apoptotic cells were histochemically demonstrated by the @entity10730 -mediated biotinylated @entity1977 nick end-labeling (TUNEL) method in @entity406 -fixed and @entity982 -embedded sections of the @entity1 endometrium and placental villi. In 53 endometrial biopsy specimens, labeled nuclei were identified in 16 samples showing a desquamating change, associated with menstruation, functional @entity548 or @entity420 . Cells in the normal proliferative and secretory phases were unlabeled. The labeled nuclei in the gland and stroma corresponded well to the so-called apoptotic bodies. Placental tissues at various stages of gestation were obtained by spontaneous @entity5852 , @entity5617 or normal delivery. Syncytiotrophoblastic cells in an early gestational stage (7-12 weeks) and in the term placenta were focally labeled, and the labeled cells possessed pyknotic nuclei and densely eosinophilic cytoplasm. In the early gestational chorionic villi with marked @entity528 or in hydatidiform mole, the stromal cells were frequently labeled. Villous cells in coagulation @entity39 ( @entity1188 ) also revealed strong signals. The apoptotic bodies were not recognizable histologically in these labeled villi. The placenta at the 20th to 33rd week of gestation lacked labeling. From a technical point of view, it should be noted that cells in the foci showing @entity63 or coagulation @entity39 were labeled positively.
