Adrenergic modulation of sharp wave-ripple activity in XXXX hippocampal slices.

@entity1249 @entity141 @entity3044 @entity2647 @entity1251 @entity35 @entity1063
@entity1063 (NE) has been shown to facilitate learning and memory by modulating synaptic plasticity in the hippocampus in vivo. During memory consolidation, transiently stored information is transferred from the hippocampus into the cortical mantle. This process is believed to depend on the generation of sharp wave-ripple complexes (SPW-Rs), during which previously stored information might be replayed. Here, we used @entity35 hippocampal slices to investigate neuromodulatory effects of NE on SPW-Rs, induced by a standard long-term potentiation (LTP) protocol, in the @entity1251 and @entity1249 . NE (10-50  M) dose-dependently and reversibly suppressed the generation of SPW-Rs via activation of a1 adrenoreceptors, as indicated by the similar effects of @entity2647 (100  M). In contrast, the unspecific b adrenoreceptor agonist @entity3044 (2  M) significantly increased the incidence of SPW-Rs. Furthermore, b adrenoreceptor activation significantly facilitated induction of both LTP and SPW-Rs within the @entity1251 network. Suppression of SPW-Rs by NE was associated with a moderate hyperpolarization in the majority of @entity1251 pyramidal cells and with a reduction of presynaptic @entity141 uptake in the stratum radiatum. This was indicated by activity-dependent changes in [ @entity141 ](o) and @entity141 fluorescence signals, by changes in the paired pulse ratio of evoked EPSPs and by analysis of the coefficient of variance. In the presence of NE, repeated high frequency stimulation (high-frequency stimulation (HFS)) failed to induce SPW-Rs, although SPW-Rs appeared following washout of NE. Together, our data indicate that the NE-mediated suppression of hippocampal SPW-Rs depends on a1 adrenoreceptor activation, while their expression and activity-dependent induction is facilitated via b1-adrenoreceptors.
