@entity1138 modulates the circadian oscillation of XXXX gene @entity17989 expression through attenuating the glucocorticoid signaling in @entity19 fibroblasts.

@entity17989 @entity14733 @entity14731 @entity9170 @entity19 @entity1138 @entity726 @entity17067 @entity1139 @entity619 @entity74 @entity18339 @entity14734 @entity14735
UNASSIGNED: @entity14731 gene regulates the circadian rhythm of various physiological functions. The expression of @entity14731 gene has been shown to be attenuated by certain drugs, resulting in a @entity74 . @entity1138 ( @entity1139 ) is often used in combination with ophthalmic surgery, especially in trabeculectomy, a @entity726 surgical procedure. The purpose of this study was to investigate the influence of @entity1139 on @entity14731 gene expression in fibroblasts, the target cells of @entity1139 . Following @entity1139 treatment, @entity14734 mRNA levels was significantly decreased, whereas @entity14735 , @entity14733 , and @entity18339 -erba mRNA levels were significantly increased in the @entity19 fibroblast cell line NIH3T3 cells. Microarray analysis was performed to explore of the gene(s) responsible for @entity1139 -induced alteration of @entity14731 gene expression, and identified @entity17067 gene encoding glucocorticoid receptor (GR) as a candidate. @entity1139 suppressed the induction of @entity14733 mRNA by @entity619 ( @entity9170 ), ligand of GR, in NIH3T3 cells. @entity1139 also modulated the @entity9170 -driven circadian oscillations of @entity17989 ::Luciferase bioluminescence in @entity19 -derived ocular fibroblasts. Our results demonstrate a previously unknown effect of @entity1139 in GR signaling and the circadian @entity14731 system. The present findings suggest that @entity1139 combined with trabeculectomy could increase the risk for a local circadian @entity74 at the ocular surface.
