Multiplicity of XXXX -inducible cytochromes P-450 in @entity35 liver microsomes.

@entity1447 @entity1472 @entity11182 @entity3208 @entity1975 @entity134 @entity7570 @entity109 @entity2975 @entity22097 @entity35
Highly purified preparations of @entity134 -inducible cytochromes P-450 have been isolated from liver microsomes of adult male @entity35 treated with @entity22097 ( @entity22097 ), @entity109 (PB), or @entity11182 ( @entity11182 ). The interrelationships among these preparations as well as their relationship to the major forms of @entity1447 of this gene family identified in other laboratories have been evaluated by @entity1472 -terminal sequence analysis, @entity3208 - @entity2975 gel electrophoresis, chromatography on DEAE-Sephacel, and Ouchterlony double-immunodiffusion. The results indicate that a @entity1447 previously isolated in this laboratory and referred to as @entity1975 (P.E. Graves, L.S. Kaminsky, and J. Halpert (1987) Biochemistry 26, 3887-3894) is the major @entity1447 induced in @entity35 by @entity11182 , and corresponds to P-450p (K.A. Hostetler, S.A. Wrighton, P. Kremers, and P.S. Guzelian (1987) Biochem. J. 245, 27-33) and to @entity7570 (F.J. Gonzalez, B.J. Song, and J.P. Hardwick (1986) Mol. Cell. Biol. 6, 2969-2976). A second previously isolated @entity1447 termed PCNb in this laboratory appears to be identical to PB/ @entity22097 -E (F.P. Guengerich, G.A. Dannan, S.T. Wright, M.V. Martin, and L.S. Kaminsky (1982) Biochemistry 21, 6019-6030). PCNb as well as a third @entity1447 termed PCNc isolated from PB-treated @entity35 both correspond in @entity1472 -terminal sequence to the putative protein product of the pP450PCN2/cDNA clone of Gonzalez et al. These results document at the protein level the multiplicity of @entity134 -inducible @entity35 liver cytochromes P-450.
