[Relationship between histone acetylation modification in spleen @entity1961 (+)T cells and XXXX of nonobese @entity6 @entity19 ].

@entity8 @entity6 @entity2316 @entity19 @entity71852 @entity1961 @entity35594 @entity1011 @entity413
OBJECTIVE: To explore the relationship between histone acetylation modification in spleen @entity1961 (+)T cells and @entity2316 . METHODS: Non-obese @entity6 (NOD) @entity19 were randomly divided into 4 groups and random blood @entity413 at 12, 18, 24 and 30 weeks were detected. Urinary albumin and @entity1011 were detected by enzyme-linked immunosorbent assay (ELISA). The global acetylation levels of H3 and H4 in @entity1961 (+)T cells were measured by H3/H4 acetylation kit. The relevant mRNA expression level of histone acetylation modification enzymes was detected by real-time quantitative polymerase chain reaction (PCR) and the altered expression genes verified by Western blot. RESULTS: Compared with @entity19 from 12 weeks, there were significant increases in blood @entity413 levels and urinary albumin/ @entity1011 ratio (ACR) from 24 and 30 weeks ( @entity413 : (18.1    6.3) and (20.7    7.5) vs (7.2    3.1)mmol/L, ACR: (4.04    1.54) and (8.11    1.77) vs (2.12    0.56)mg/g). Conversely, the global acetylation levels of H3 (0.068    0.023 and 0.043    0.017 vs 0.127    0.036) and H4 (0.058    0.022 and 0.041    0.019 vs 0.082    0.032) in spleen @entity1961 (+)T cells from 24 and 30 weeks were obviously lower. The mRNA levels of such histone acetylases as P300 (15.53    6.31 and 13.76    3.62 vs 22.94    7.40) and @entity71852 ( @entity71852 ) (3.21    0.81 and 2.74    0.36 vs 5.31    0.73) declined while the protein and mRNA of @entity35594 ( @entity35594 ) significantly increased from 24 and 30 weeks. All the above differences were statistically significant (P < 0.05). The levels of H3 (r = -0.590, P = 0.043) and H4 (r = -0.702, P = 0.011) were negatively correlated with urinary ACR while @entity35594 level was positively correlated with urinary ACR from 24 and 30 weeks (r = 0.673, P = 0.016). CONCLUSIONS: With aging and progression of @entity8 in NOD @entity19 , the global acetylation levels of H3 and H4 decrease while the expression level of @entity35594 increases in spleen @entity1961 (+)T cell. And altered @entity35594 expression in spleen @entity1961 (+)T cells is closely correlated with @entity8 in NOD @entity19 .
