The nude XXXX for the study of @entity1 @entity14 .

@entity1 @entity5 @entity19 @entity3 @entity14
The tumorigenicity and metastatic behaviour of @entity1 @entity14 have been assessed in athymic @entity19 . Surgical specimens were obtained from @entity14 or from @entity3 of different @entity1 . Primary @entity14 and their synchronous liver @entity3 were obtained from three @entity1 . After intramuscular (i.m.) injection into @entity19 , @entity5 cells originating from @entity3 showed a higher take than those from @entity5 . In general, metastasis-derived @entity5 had a shorter doubling time than primary-derived @entity5 . This growth pattern was not associated with the production of spontaneous @entity3 in @entity19 . To investigate the malignant potential of @entity14 in the nude @entity19 , @entity5 cells were injected intravenously (i.v.) (artificial lung @entity3 ) and in the spleen (artificial liver @entity3 ). Colorectal-derived @entity5 cells produced lung colonies after i.v. injection, but the most dramatic expression of @entity5 was the @entity5 cells' ability to grow in the liver after intrasplenic (i.s.) injection. The ability to grow in the liver of @entity19 appeared to be a distinct characteristic of each @entity5 cell population. @entity1 @entity14 were maintained as continuous lines subcutaneously (s.c.) in @entity19 . Phenotypic differences, including malignant potential, were generally maintained at different passages. @entity1 @entity14 with a definite pattern of malignant behaviour (i.e. to the liver) in the nude @entity19 provide a unique experimental system for the study of the biology and the therapy of cancer @entity3 .
