Bexarotene Reduces Blood-Brain Barrier Permeability in @entity2806 -Reperfusion Injured XXXX .

@entity1 @entity22677 @entity2806 @entity63 @entity75 @entity12217 @entity659 @entity6929 @entity35
BACKGROUND: @entity6929 ( @entity6929 ) over-expression disrupts the blood-brain barrier (BBB) in the @entity63 brain. The retinoid X receptor agonist bexarotene suppresses @entity6929 expression in endothelial cells and displays neuroprotective effects. Therefore, we hypothesized that bexarotene may have a beneficial effect on I/R-induced @entity75 . METHODS: A total of 180 @entity35 were randomized into three groups (n = 60 each): (i) a sham-operation group, (ii) a @entity2806 -reperfusion (I/R) group, and (iii) an I/R+bexarotene group. Brain water content was measured by the dry wet weight method. BBB permeability was analyzed by Evans Blue staining and the magnetic resonance imaging contrast agent Omniscan. @entity6929 mRNA expression, protein expression, and activity were assessed by reverse transcription polymerase chain reaction, Western blotting, and gelatin zymography, respectively. @entity12217 ( @entity12217 ), @entity22677 , and @entity659 expression were analyzed by Western blotting. RESULTS: After 24 h, 48 h, and 72 h post-I/R, several effects were observed with bexarotene administration: (i) brain water content and BBB permeability were significantly reduced; (ii) @entity6929 mRNA and protein expression as well as activity were significantly decreased; (iii) @entity22677 and @entity659 expression were significantly increased; and (iv) @entity12217 expression was significantly increased. CONCLUSIONS: Bexarotene decreases BBB permeability in @entity35 with cerebral I/R injury. This effect may be due in part to bexarotene's upregulation of @entity12217 expression, which has been previously shown to reduce BBB permeability through suppressing @entity6929 -mediated degradation of the tight junction proteins @entity22677 and @entity659 . This work offers insight to aid future development of therapeutic agents for cerebral I/R injury in @entity1 @entity1 .
