Analysis of a salinity induced BjSOS3 protein from Brassica indicate it to be structurally and functionally related to its ortholog from XXXX .

@entity30431 @entity958 @entity26911 @entity460 @entity141 @entity13344
@entity958 has been a favorite model system for plant biologist. It is anticipated that comparative analysis of this plant with other members of Brassicaceae may aid in identification of orthologs playing role as key genetic determinants for salinity response. In this endeavor, we have recently identified SOS family members from @entity13344 in our laboratory and reported their salinity responsive transcriptional induction in seedlings of various diploid and amphidiploids species. In the present study, we have carried out detailed time kinetics for BjSOS3 expression in a salinity tolerant @entity13344 . CS52. Transcript analysis at the sensitive growth stages of plants viz. seedling and reproductive stage indicated clear differential transcriptional regulation of BjSOS3 under non-induced as well as salinity induced conditions in a time and organ specific manner, mirroring their respective tolerance physiology. Similar to its ortholog from @entity958 , the modeled BjSOS3 protein show typical features of a @entity141 binding protein with four conserved EF-hands. We have also attempted to study the binding of @entity30431 protein with the modeled @entity26911 protein. It has been established that @entity30431 protein senses @entity141 though the binding is very weak; we show the down regulation of BjSOS3 mRNA in presence of @entity460 chelator - EGTA under the various stress conditions including ABA. In situ localization of BjSOS3-GFP fusion protein in onion peel has shown its presence strongly in plasma membrane as well as cytosol. The leads presented in the paper will assist in understanding and establishing the SOS signaling machinery in @entity13344 .
