Characterization of @entity24585 fragments produced by XXXX .

@entity291 @entity535 @entity2733 @entity2340 @entity24585 @entity59907 @entity1473
Cleavage of @entity24585 by @entity59907 was studied. Four primary products were detected and separated by high performance liquid chromatography. Two of the fragments are fluorescent and therefore contain residue 23 ( @entity2340 ). These fragments are @entity2733 -terminal in origin. The other two cross-react with antisera directed against @entity1473 -terminal portions of the hormone; they are the complementary @entity1473 -terminal fragments. Microsequencing and @entity535 analysis showed that the two @entity1473 -terminal fragments are 35-84 and 38-84 @entity291 @entity24585 . By CNBr cleavage and @entity535 analysis, the two @entity2733 -terminal fragments were shown to be the complementary 1-37 and 1-34 fragments. The 1-37 fragment is transitory and is rapidly hydrolyzed to 1-34, so that only relatively small amounts are detected at any one time. However, 34-84 was not converted to 38-84, although cleavage at other sites in the @entity1473 -terminal fragments was observed with more exhaustive digestion. The 1-34 fragment appears to be the final product of the action of @entity59907 on @entity24585 . Both enzymatically produced @entity2733 -terminal fragments were fully active in the renal membrane adenylyl cyclase assay system.
