A mutation affecting the @entity880 dehydrogenase locus XXXX in the @entity19 -I. Genetical and electrophoretical characterization.

@entity4174 @entity19 @entity73028 @entity2975 @entity880 @entity36904
(101/El x C3H/el)F1 male @entity19 were infected intraperitoneally with the mutagen @entity4174 and immediately caged with untreated test-stock females. Crude liver extracts from the offspring were subjected to @entity2975 gel isoelectric focusing, and the gels were stained for six enzymes. In the experimental group (mutagen treated spermatogonial germ-cell stage), a dominant inherited banding alteration of the @entity880 dehydrogenase (LDH) pattern was detected. By crossing the heterozygous mutants, homozygotes were obtained that showed much less gel staining intensity. The mutation is codominantly expressed with 100% penetrance. The banding alteration was also observed in muscle, kidney, heart, blood, brain, testis, spleen, and lung. @entity2975 gel electrophoresis was performed with all the tissues examined. The mutation causes the intensity of the band corresponding to @entity73028 (primary molecular form in muscle) to decrease from that of the wild type, while the intensity of the bands corresponding to @entity36904 (primary molecular form in heart) remains constant. It is concluded that the mutation affects the locus coding for LDH of the muscle type. Ldh-1c is proposed as the allele symbol.
