Role of XXXX in induction of @entity842 synthase.

@entity2036 @entity19 @entity26187 @entity60 @entity842 @entity13633 @entity7375 @entity141
@entity60 ) interacts synergistically with bacterial lipopolysaccharide (LPS) to induce transcription of @entity13633 , the isoform of @entity842 synthase whose activity is independent of elevated @entity141 and exogenous calmodulin. To define a cis-acting element mediating @entity60 -dependent synergy, we made deletions in @entity13633 promoter constructs fused to reporter genes, transfected RAW 264.7 macrophages, and treated the cells with @entity60 and/or LPS. This analysis implicated the region from positions -951 to -911, a cluster of four enhancer elements known to bind @entity60 -responsive transcription factors, including an interferon regulatory factor binding site (IRF-E) at @entity2036 -913 to -923. Site-specific substitution of two conserved @entity2036 within IRF-E in the context of the full-length @entity13633 promoter ablated @entity60 's contribution to synergistic enhancement of transcription. Electromobility shift assays performed with a probe containing IRF-E revealed the existence of a complex in nuclei of RAW 264.7 macrophages that was present only after treatment with @entity60 , which reacted specifically with anti- @entity26187 immunoglobulin G and which included a species migrating at 40-45 kD, consistent with the apparent molecular weight of @entity19 @entity26187 . Thus, the synergistic contribution of @entity60 to transcription of @entity13633 in RAW 264.7 macrophages requires that @entity26187 bind to IRF-E in the @entity13633 promoter. In conjunction with the work of Kamijo et al. (Kamijo, R., H. @entity7375 , T. Matsuyama, M. Bosland, J. Gerecitano, D. Shapiro, J. Le, K. S. Im, T. Kimura, S. Green et al. 1994. Science [Wash. DC]. 263:1612), these findings identify @entity13633 as the first gene that requires @entity26187 for @entity60 -dependent transcriptional regulation.
