[Expression of orexin receptor 1 in XXXX pancreatic tissue with @entity28 ].

@entity28 @entity412 @entity35 @entity24210 @entity413 @entity165
OBJECTIVE: To identify the presence of orexin receptor 1 (OXR1) in @entity28 @entity35 pancreas and clarify its effect and mechanism on body weight and metabolic index in @entity28 @entity35 . METHODS: The @entity28 @entity35 model was established a high-fat diet. And the serum levels of @entity413 , @entity412 and insulin were measured by Mini-Blood @entity413 meter, biochemical enzymatic method and chemiluminescent immunoassay. The pancreatic expression of @entity24210 protein was identified by immunohistochemistry. After an intracerebroventricular infusion of orexin receptor 1 antisense oligodeoxynucleotide ( @entity24210 -PS-ODNs), the effect on @entity24210 protein was detected in @entity35 pancreatic tissue with @entity28 . RESULTS: After feeding for 8 weeks, high-fat diet @entity35 appear @entity28 body weight, serum @entity413 , insulin, @entity412 and @entity165 of high-diet @entity35 were higher than those in the control group (P < 0.05). The expression of @entity24210 protein was located in the cytoplasm of pancreas Islet b-cells. The expression of @entity24210 protein in @entity28 @entity35 pancreas decreased around 16% versus the normal group (P < 0.05). After the @entity28 @entity35 were treated by @entity24210 -PS-ODNs for 72 hours, the expression of @entity24210 protein in pancreas declined from 82    6 to 65    4, reduced about 21% compare with control group (P < 0.01). CONCLUSION: The expression of @entity24210 protein is found in pancreas Islet b-cells. Hypothalamic orexin system can intervene the expression of @entity24210 protein in @entity35 pancreatic tissue with @entity28 . And it may participate in the occurrence of @entity28 and the metabolic regulation of serum @entity413 and insulin.
