The in vitro synthesis of estrogen-dependent proteins by the XXXX ( @entity3474 ) oviduct.

@entity1 @entity2198 @entity1420 @entity3474 @entity4823 @entity1853 @entity53 @entity2975
This study was undertaken to determine the effect of @entity53 and @entity1420 on the synthesis of oviduct-specific proteins and to determine if there were changes in the synthesis of these proteins in different regions of the oviduct. Ovariectomized females were either untreated, treated with @entity53 for 7 or 14 days, or primed with @entity53 for 14 days and then treated with @entity53 plus @entity1420 or @entity1420 alone for either 7 or 14 days. Oviducts were incubated in the presence of labeled @entity1853 or @entity4823 for 24 h at 37 C, and the culture medium was then analyzed by one- and two-dimensional (2-D) @entity2975 gel electrophoresis, followed by fluorography. A family of macromolecules [mol wt (Mr), 100,000-130,000] was only present in the animals treated with @entity53 for 7 or 14 days. 2-D analysis demonstrated that two proteins, one basic and one acidic, were the major @entity53 -responsive proteins in the 100,000-130,000 Mr region. In addition, an acidic protein in this region increased in intensity with @entity53 treatment. All three proteins incorporated @entity1853 and @entity4823 . Since a steroid-responsive gradient is known to exist in the oviduct, oviducts were divided into fimbria, ampulla, and isthmic regions and cultured separately. 2-D analysis of the medium indicated that the ampulla synthesized both the @entity2198 , whereas the acidic protein was dominant in the fimbria and the basic protein was dominant in the isthmus. Another acidic protein (Mr, 110,000) was only present in the fimbria and ampulla. This study clearly demonstrates that the @entity53 -treated @entity3474 oviduct synthesizes several proteins that were not detectable in the ovariectomized or @entity1420 -treated animal. Also, the different regions of the oviduct have different synthetic capabilities for these proteins. The electrophoretic characteristics of these proteins are similar to those we have observed at midcycle in the intact @entity3474 and @entity1 oviduct.
