Proteomics in XXXX research.

@entity1 @entity263 @entity5 @entity1486
The incidence of @entity263 ( @entity263 ) among middle-aged @entity1 has increased rapidly. For many of these @entity1 , curatively intended treatment does more harm than good. Established prognostic factors are @entity5 stage and grade. As a result of earlier detection a majority of @entity1 now have nonpalpable @entity5 (T1c) of intermediate grade (Gleason score 6). @entity1486 in serum in such cases is generally at a low level and not a reliable predictor of prognosis. Altogether there is an urgent need for adjunctive prognostic indicators. In the search for relevant @entity5 markers for improved @entity1 selection an exploration of the proteome (the @entity1 proteins) could be fruitful. This paper critically reviews the use of 2-dimensional gel electrophoresis (2-DE) for proteome research. Additional steps such as image analysis and mass spectrometry are described. Techniques based on non-2-DE platforms: surface-enhanced laser desorption/ionization (SELDI), isotope coded affinity tags (ICAT) and array-based technologies are also summarized. Although labor-intensive and time-consuming, 2-DE is presently the most powerful method for analysis of cellular protein phenotype and may potentially reveal gene regulations that cannot be detected on a genetic level.
