Telomerized XXXX multipotent mesenchymal cells can differentiate into hematopoietic and cobblestone area-supporting cells.

@entity1 @entity2394 @entity14485 @entity12359 @entity1583 @entity2280 @entity2215
OBJECTIVE: To compare the hematopoietic support provided by telomerized @entity1 mesenchymal stem cells (MSCs) and telomerized MSC-derived stromal cells. METHODS: We transfected the @entity1 @entity2215 (hTERT) gene into primary MSCs to establish hTERT-transduced MSCs (hTERT-MSCs). Stromal induction of hTERT-MSCs was performed by replacing the culture medium with Dexter-type culture medium. @entity1583 support was examined by coculture with cord blood @entity2280 (+) cells. RESULTS: The hTERT-MSCs were morphologically identical with the primary MSCs and expressed surface antigens including CD105, @entity12359 , and @entity2394 . hTERT-MSCs showed a similar doubling time as primary MSCs and continued to proliferate to over 80 @entity14485 ( @entity14485 ), although the primary MSCs underwent crisis in vitro at 16 @entity14485 . The osteogenic, chondrogenic, adipogenic, neurogenic, and stromal differentiation potential of hTERT-MSCs were maintained up to at least 40 @entity14485 . The degree of expansion of @entity2280 (+) cells and total number of colony-forming units in culture (CFU-C) upon 12-day coculture with the hTERT-MSC-derived stromal cells were nearly the same as those upon 12-day coculture with hTERT-MSCs ( @entity2280 , 33.0-fold+/-2.8-fold vs 36.1-fold+/-1.7-fold of the initial cell number; CFUs, 344.4-fold+/-62.5-fold vs 239.3-fold+/-87.0-fold; CFU-mix, 368.4-fold+/-113.7-fold vs 341.3-fold+/-234.3-fold). However, on day 18 of coculture, the number of cobblestone areas (CA) observed beneath the stromal cells was 15 times higher than that beneath hTERT-MSCs (CA, 146.9+/-54.6 vs 9.4+/-8.1, p<0.01). CONCLUSION: Stromal induction of hTERT-MSCs exclusively enhanced the support of CA formation provided by hTERT-MSCs. Our @entity1 hTERT-MSCs will be useful for elucidating the mechanism of the formation of CAs.
