Neoplastic modulation of extracellular matrix: proteoglycan changes in the @entity95 mesentery induced by V2 XXXX cells.

@entity13275 @entity5 @entity13326 @entity597 @entity95 @entity189 @entity12006 @entity4023
Previous studies have shown that the invasion of V2 @entity189 cells in the @entity95 mesentery is associated with marked extracellular matrix synthesis leading eventually to an overall increase in mesenteric mass. The purpose of the present study was to investigate the structural and biochemical composition of the extracellular matrix in @entity5 -free parts of @entity95 mesenteries at various stages after intraperitoneal implantation of V2 @entity189 cells. The overall thickness of the @entity5 -implanted mesenteries increased progressively and peaked at about Day 14, when it was about 8 times greater than the untreated or liver-implanted controls. This was mainly the result of an accumulation of extracellular matrix components. In particular, there was a marked increase in both collagen fibers and proteoglycan granules, as well as filaments, probably @entity4023 , as visualized by @entity13326 . Stereological analysis showed a 6-fold increase in collagen fibers and a significant increase in the density and average diameter of proteoglycan granules. Biochemical analysis revealed a marked elevation in @entity13275 content in the @entity5 -implanted mesenteries. Specifically, they contained 2.6 and 8.6 times the amount of @entity4023 and @entity12006 , respectively, than did controls. Furthermore, the relative percentage of @entity12006 was elevated markedly (26 versus 6% in controls). However, the content of heparan or dermatan @entity597 did not vary significantly. Stereological analysis of the fibroblasts showed that their absolute number had doubled and that the cell volume of the individual fibroblast had increased markedly. This suggests that the fibroblasts were responsible for the excessive production of the extracellular matrix. These results support the concept that @entity189 cells can modulate their surrounding extracellular environment by stimulating the synthesis of connective tissue in the host mesenchymal cells.
