XXXX responsiveness of stromal cells of the @entity1 prostate: regulation of cell proliferation and keratinocyte growth factor by @entity342 .

@entity1 @entity586 @entity10471 @entity665 @entity342 @entity587
PURPOSE: Growth and development of the prostate are @entity342 dependent and mainly influenced by stromal-epithelial interaction. It is believed that indirect androgenic activation of paracrine factors like keratinocyte growth factor ( @entity10471 ) in the prostatic stroma influences the growth of epithelial cells. In this study we investigated the role @entity342 plays in stromal cell growth and stimulation of @entity10471 in the @entity1 prostate. MATERIALS AND METHODS: Stromal cells were derived from explant primary culture of @entity1 normal or benign prostatic tissue. The effect of different @entity586 ( @entity587 ) concentrations on cell proliferation was measured using @entity665 incorporation assay. The effect of @entity587 on levels of @entity10471 protein was determined by Western blotting. The effect of @entity587 on levels of @entity10471 gene expression was measured by various cycles of polymerase-chain-reaction (PCR) and multiplex PCR. RESULTS: Characterization of stromal cells showed epithelial cells less than 9.5% in all passages. @entity587 stimulated @entity1 prostate stromal cells in a dose dependent fashion over a concentration range of 0.001-10 nM. Immunocytochemical evaluation of @entity10471 after @entity587 exposure showed a higher staining intensity. Relative quantitation of Western blotting showed a 1.93-fold increase in @entity10471 protein in the @entity342 treated stromal cells. At 1 nM @entity587 conventional and multiplex PCR revealed a significant stimulation of the @entity10471 mRNA expression. CONCLUSIONS: These data show for the first time that @entity342 stimulates cell proliferation as well as @entity10471 protein and gene expression in @entity1 prostate stromal cells. This supports the hypothesis that @entity342 -induced stromal-derived @entity10471 stimulates prostate epithelial cell growth.
