XXXX Umbilical Cord Mesenchymal Stem Cells Inhibit the Function of Allogeneic Activated Vy9V  2 T Lymphocytes In Vitro.

@entity1 @entity137 @entity5279 @entity2205 @entity12 @entity2975 @entity1199
BACKGROUND: @entity1 umbilical cord mesenchymal stem cells (UC-MSCs) can regulate the function of immune cells. However, whether and how UC-MSCs can modulate the function of Vy9V  2 T cells has not been fully understood. METHODS: The PBMCs or Vy9V  2 T cells were activated and expanded with @entity5279 ( @entity2975 ) and @entity1199 ( @entity1199 ) with or without the presence UC-MSCs. The effects of UC-MSCs on the proliferation, cytokine expression, and @entity137 of Vy9V  2 T cells were determined by flow cytometry. The effects of UC-MSCs on @entity12 , @entity2205 -expressing Vy9V  2 T cells, and Vy9V  2 T cell apoptosis were determined by flow cytometry. RESULTS: UC-MSCs inhibited Vy9V  2 T cell proliferation in a dose-dependent but cell-contact independent manner. Coculture with UC-MSCs reduced the frequency of IFNy+ but increased granzyme B+ Vy9V  2 T cells. UC-MSCs inhibited the @entity137 of Vy9V  2 T cells against influenza virus H1N1 infected A549 cells and also reduced the frequency of @entity12 +, @entity2205 + Vy9V  2 T cells but failed to modulate the apoptosis of Vy9V  2 T cells. CONCLUSIONS: These results indicated that UC-MSCs efficiently suppressed the proliferation and @entity137 of Vy9V  2 T cells and modulated their cytokine production. @entity12 and @entity2205 were involved in the regulation. Cell contact and apoptosis of Vy9V  2 T cells were not necessary for the inhibition.
