@entity4514 -induced injury of isolated perfused XXXX lungs: neutrophil dependence.

@entity4515 @entity2507 @entity35 @entity4514 @entity647
The effect of leukocyte depletion on @entity647 produced by intravenous or intratracheal @entity4514 ( @entity4515 ) administration was studied in isolated perfused @entity35 lungs. Vascular endothelial permeability was assessed by use of the capillary filtration coefficient (Kf,c). A predicted pulmonary capillary pressure (Ppc,p) was calculated from measurements of postcapillary resistances. These parameters were measured before and 90 min after the administration of @entity4515 , either intratracheally or intravascularly. When blood elements were present both intratracheal and intravascular @entity4515 caused an increased Kf,c [0.27 +/- 0.02 vs. 0.99 +/- 0.22 and 0.25 +/- 0.05 vs. 0.64 +/- 0.15 (SE) ml.min-1.cmH2O-1.100 g-1, respectively; P less than 0.05] and an increased Ppc,p (8.3 +/- 0.4 vs. 74.7 +/- 18.3 and 8.7 +/- 0.8 vs. 74.2 +/- 25.1 cm @entity2507 , respectively; P less than 0.05). Removal of circulating leukocytes abolished the increased Kf,c when @entity4515 was given intratracheally (0.35 +/- 0.06 vs. 0.23 +/- 0.07 ml.min-1.cmH2O-1.100 g-1) or intravascularly (0.39 +/- 0.07 vs. 0.33 +/- 0.07 ml.min-1.cmH2O-1.100 g-1). In the absence of neutrophils, Ppc,p slightly increased with intratracheal @entity4515 , from 6.9 +/- 0.5 to 10.5 +/- 1.1 cm @entity2507 (P less than 0.05), but was unchanged at 90 min with intravascular @entity4515 . Depletion of circulating neutrophils with an antineutrophil serum failed to block the Kf,c change with intratracheal @entity4515 (from 0.24 +/- 0.03 to 0.42 +/- 0.09 ml.min-1.cmH2O-1.100 g-1; P less than 0.05). Ppc,p also increased from 6.9 +/- 0.6 to 19.8 +/- 6.7 cm @entity2507 (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
