Coagulation factor II from rock bream ( XXXX ): First report on the molecular biological function and expression analysis in the teleost.

@entity3534 @entity9855 @entity548 @entity39745 @entity1003 @entity281 @entity16699 @entity563 @entity34938 @entity526
UNASSIGNED: The rapid haemostasis of fish prevents @entity548 that could be caused by physical properties of the aquatic environment. Additionally, the innate immune system is the first line of defence against @entity281 and is responsible for the recognition of pathogen-associated molecular patterns, which are important for the activation of acquired immune responses. Coagulation factor II (CFII) is an important factor in the coagulation system and is involved in recognition and interaction with various bacterial and extracellular proteins. In this study, we identified and characterised the gene encoding CFII in rock bream ( @entity39745 ) (RbCFII) and analysed its expression in various tissues after a pathogen challenge. The full-length RbCFII cDNA (2079  bp) contained an open reading frame of 1854  bp encoding 617 @entity3534 . Alignment analysis revealed that a @entity16699 -rich domain, two kringle domains, and a trypsin-like @entity563 protease domain of the deduced protein were well conserved. RbCFII was ubiquitously expressed in all tissues examined but, predominantly detected in the liver and skin. RbCFII expression was dramatically up-regulated in the kidney, spleen and liver after @entity281 with @entity34938 , Streptococcus iniae, or @entity9855 iridovirus. The recombinant protein RbCFII (rRbCFII) produced using an @entity526 expression system was able to bind all examined bacteria. Interestingly, rRbCFII has agglutination activities towards @entity526 and @entity34938 , while no agglutination was shown toward Vibrio ordalii and S.  iniae. These findings indicate that rRbCFII performs an immunological function in the immune response, and might be involved in innate immunity as well as @entity1003 .
