Analysis of XXXX sensu lato isolated from cerebrospinal fluid.

@entity1 @entity3001 @entity16 @entity2069 @entity21786 @entity8831 @entity281 @entity272 @entity25974
Involvement of the nervous system in @entity3001 may occur with or without @entity8831 and it may present with a variety of @entity16 . In this study we analysed phenotypic and genotypic characteristics of 40 Borrelia strains isolated from cerebrospinal fluid (CSF) of 38 Slovenian @entity1 with different clinical manifestations of @entity3001 . In seven of the @entity1 , Borreliae were also isolated from @entity272 . Species identification and plasmid profiles were determined by pulsed-field gel electrophoresis and protein profiles by SDS-PAGE. MluI digestion profiles of @entity2069 sensu lato DNA showed that 25 (62.5%) isolates were @entity25974 , 14 (35%) @entity21786 , and one (2.5%) @entity2069 . All strains, except one, possessed a large plasmid and a varying number of smaller plasmids. Three (7.5%) isolates exhibited an unusual plasmid profile, with a large plasmid dimer or three copies of the large plasmid. In protein analyses, all strains expressed OspA protein. OspB was present significantly more often in @entity21786 than @entity25974 strains (p=0.0000), while OspC was more often present in @entity25974 than @entity21786 strains (p=0.0052). In the seven @entity1 with Borreliae isolated also from the skin, the CSF and skin isolates were identical, either @entity25974 (six @entity1 ) or @entity21786 (one @entity1 ). Species and plasmid heterogeneity as well as antigen diversity could play a role in the pathogenesis of the @entity281 . When combined with our own earlier data, the results suggest species-related organotropism.
