Identification of XXXX Homeostasis Genes Dysregulation Potentially Involved in @entity989 Pathogenicity by Microarray Analysis.

@entity1 @entity5303 @entity26 @entity519 @entity989 @entity68
@entity989 ( @entity989 ) is a serious disease of preterm neonates and there are limited systematic studies of the molecular mechanisms underlying @entity989 . Therefore, here we performed global gene expression profiling in @entity1 fetal retinal microvascular endothelial cells (RMECs) under @entity519 conditions in vitro. Aborted fetuses were enrolled and primary RMECs were isolated from eyeballs. Cultivated cells were treated with @entity5303 to induce @entity519 . The dual-color microarray approach was adopted to compare gene expression profiling between treated RMECs and the paired untreated control. The one-class algorithm in significance analysis of microarray (SAM) software was used to screen the differentially expressed genes (DEGs) and quantitative RT-PCR (qRT-PCR) was conducted to validate the results. Gene Ontology was employed for functional enrichment analysis. There were 326 DEGs between the @entity519 -induced group and untreated group. Of these genes, 198 were upregulated in @entity519 RMECs, while the other 128 hits were downregulated. In particular, genes in the @entity68 ion homeostasis pathway were highly enriched under @entity519 conditions. Our study indicates that dysregulation of genes involved in @entity68 homeostasis mediating oxidative damage may be responsible for the mechanisms underlying @entity989 . The " @entity26 plus @entity68 hypothesis may improve our understanding of @entity989 pathogenesis.
