[Effect of shenmai injection and @entity3416 on small airway smooth muscle cell apoptosis and related gene expression in @entity35 with XXXX ].

@entity35 @entity23975 @entity3416 @entity2415
OBJECTIVE: To investigate the effect of Shenmai Injection (SMI) and @entity3416 on small airway smooth muscle cell (SASMC) apoptosis and the Fas/ @entity23975 expression in the papain induced @entity2415 model @entity35 . METHODS: @entity2415 model in @entity35 was established by a single intratracheal instillation of papain. Apoptosis and Fas/ @entity23975 expression of SASMC were examined by immunohistochemical SABC and TUNEL assay at 1, 3, 5, 7, 15 and 30 days after modelling, and the effect of SMI and @entity3416 on them were observed. RESULTS: Fas, @entity23975 expressions in normal SASMC were very low with a positive rate of (2.31 +/- 0.05)% and (1.28 +/- 0.47)% respectively. After papain instillation, the positive rates increased along with the prolonging of instillation time. SMI showed an inhibition on SASMC Fas and @entity23975 expression but @entity3416 didn't show. SASMC apoptosis was very low in normal @entity35 with a rate of (0.87 +/- 0.32)%, it also raised after papain instillation and increased progressively along with the instillation time. SMI treatment could lower the apoptosis rate but @entity3416 couldn't. CONCLUSION: Fas and @entity23975 participated the SASMC apoptosis modulation in @entity2415 formation. SMI shows a definite treatment effect on @entity2415 by influencing the Fas and @entity23975 protein expression and reducing SASMC apoptosis through inhibiting the release of inflammatory mediator.
