Delta-24-RGD Induces XXXX Spheroids in Three Dimensional PEG Microwells.

@entity5 @entity137 @entity463 @entity1313
UNASSIGNED: @entity463 ( @entity463 ) is the most aggressive @entity1313 , with 12-15 months median survival time despite current treatment efforts. Among the alternative treatment approaches that have gained acceptance over the last decade is the use of replication-competent oncolytic adenoviruses, which are promising due to their relatively low @entity137 and @entity5 -specific targeting. Three-dimensional (3D) @entity5 models can mimic the physiological microenvironment of @entity463 @entity5 and provide valuable information about the interaction between @entity5 cells and adenoviruses. Therefore, robust in vitro 3D @entity5 models are critical to investigate the mechanisms underlying @entity5 progression and explore the @entity137 effect of the adenovirus on @entity5 cells. In this study, we used a hydrogel microwell platform to generate in vitro 3D @entity463 spheroids and studied their interactions with the Delta-24-RGD adenovirus. The results showed that the cultured 3D spheroids were successfully infected by the Delta-24-RGD. A significant cell lysis was observed. Cell viability was decreased approximately 37%, 54% and 65% with 10, 50, and 100 MOIs, respectively. The infection of the Delta-24-RGD was found more effective on 3D spheroids when compared to 2D monolayer cell culture. These results implicate that our hydrogel microwell platform could provide a promising 3D model to investigate the oncolytic potential of the viruses in vitro.
