Neuropeptide Y family receptors Y1 and Y2 from XXXX , @entity26962 .

@entity1 @entity7766 @entity1942 @entity72050 @entity26962 @entity42384 @entity19260
UNASSIGNED: The vertebrate gene family for neuropeptide Y ( @entity7766 ) receptors expanded by duplication of the chromosome carrying the ancestral Y1-Y2-Y5 gene triplet. After loss of some duplicates, the ancestral jawed vertebrate had seven receptor subtypes forming the Y1 (including Y1, Y4, Y6, Y8), Y2 (including Y2, Y7) and Y5 (only Y5) subfamilies. Lampreys are considered to have experienced the same chromosome duplications as gnathostomes and should also be expected to have multiple receptor genes. However, previously only a Y4-like and a Y5 receptor have been cloned and characterized. Here we report the cloning and characterization of two additional receptors from the @entity26962 @entity26962 . Sequence phylogeny alone could not with certainty assign their identity, but based on synteny comparisons of @entity42384 and the Arctic lamprey, Lethenteron camtschaticum, with jawed vertebrates, the two receptors most likely are Y1 and Y2. Both receptors were expressed in @entity1 HEK293 cells and @entity19260 assays were performed to determine the response to the three native lamprey peptides @entity7766 , @entity1942 and PMY. The three peptides have similar potencies in the nanomolar range for Y1. No obvious response to the three peptides was detected for Y2. Synteny analysis supports identification of the previously cloned receptor as Y4. No additional @entity7766 receptor genes could be identified in the presently available lamprey genome assemblies. Thus, four @entity7766 -family receptors have been identified in lampreys, orthologs of the same subtypes as in @entity1 ( @entity72050 ), whereas many other vertebrate lineages have retained additional ancestral subtypes.
