Depletion of the "gamma-type carbonic anhydrase-like" subunits of complex I affects central mitochondrial metabolism in XXXX .

@entity958 @entity1792 @entity45994 @entity3158 @entity245 @entity71169 @entity71170 @entity505 @entity45995 @entity3689
UNASSIGNED: "Gamma-type carbonic anhydrase-like" (CAL) proteins form part of complex I in plants. Together with " @entity71169 (CA) proteins they form an extra domain which is attached to the membrane arm of complex I on its matrix exposed side. In @entity958 two CAL and three CA proteins are present, termed @entity71170 , CAL2, @entity45994 , @entity45995 and CA3. It has been proposed that the carbonic anhydrase domain of complex I is involved in a process mediating efficient recycling of mitochondrial @entity3158 for photosynthetic @entity505 fixation which is especially important during growth conditions causing increased photorespiration. Depletion of CAL proteins has been shown to significantly affect plant development and photomorphogenesis. To better understand CAL function in plants we here investigated effects of CAL depletion on the mitochondrial compartment. In mutant lines and cell cultures complex I amount was reduced by 90-95% but levels of complexes III and V were unchanged. At the same time, some of the CA transcripts were less abundant. Proteome analysis of CAL depleted cells revealed significant reduction of complex I subunits as well as proteins associated with photorespiration, but increased amounts of proteins participating in @entity3689 catabolism and stress response reactions. @entity245 of the mutants was slightly alleviated if plants were cultivated at high @entity3158 . Profiling of selected metabolites revealed defined changes in intermediates of the @entity1792 cycle and @entity3689 catabolism. It is concluded that CAL proteins are essential for complex I assembly and that CAL depletion specifically affects central mitochondrial metabolism.
