Interplay among Gcn5, @entity28555 and Mitochondria during Chronological Aging of Wine XXXX Is Dependent on Growth Conditions.

@entity7027 @entity12632 @entity1568 @entity71906 @entity28555 @entity3147 @entity505 @entity375 @entity49008
@entity375 @entity7027 ( @entity7027 ) is determined by a wide variety of environmental and genetic factors. Nutrient limitation without @entity3147 , i.e. dietary restriction, expands @entity7027 through the control of nutrient signaling pathways, of which TOR/ @entity28555 has proven to be the most relevant, particularly under @entity1568 deprivation. The use of prototrophic wine @entity375 allows a better understanding of the role of @entity1568 in longevity in natural and more demanding environments, such as grape juice fermentation. We previously showed that acetyltransferase Gcn5, a member of the SAGA complex, has opposite effects on @entity7027 under laboratory and winemaking conditions, and is detrimental under the latter. Here we demonstrate that integrity of the SAGA complex is necessary for prolonged longevity, as its dismantling by @entity49008 deletion causes a drop in @entity7027 under both laboratory and winemaking conditions. The @entity28555   mutant is long-lived in synthetic SC medium, as expected, and the combined deletion of GCN5 partially suppresses this phenotype. However it is short-lived in grape juice, likely due to its low @entity1568 / @entity505 ratio. Therefore, unbalance of nutrients can be more relevant for life span than total amounts of them. Deletion of @entity12632 , which codes for a protein associated with Gcn5 and is a component of the mitochondrial retrograde signal, and which communicates mitochondrial dysfunction to the nucleus, is detrimental under laboratory, but not under winemaking conditions, where respiration seems not so relevant for longevity. Transcription factor @entity71906 was found to be a novel @entity7027 regulator @entity28555 -dependently.
