Antileishmanial activity of Ferula assa-foetida oleo gum resin against XXXX : An in vitro study.

@entity3617 @entity1 @entity55 @entity5695 @entity19 @entity232 @entity11634 @entity2526 @entity3667 @entity9496
BACKGROUND: In Ayurveda, asafetida is introduced as a valuable remedy for @entity11634 , @entity9496 , @entity2526 , @entity5695 , @entity55 and @entity3617 in @entity1 and also considered as an aphrodisiac agent. Presently, @entity232 is common in most countries of the world and is a serious health problem in the world. Some plant medicines and natural products have a new candidate for treatment of @entity232 . OBJECTIVE: This study was designed to evaluate Ferula assa-foetida oleo gum resin (asafetida) on mortality and morbidity @entity3667 in vitro. MATERIALS AND METHODS: Mostigotes were isolated from @entity19 spleens and then transformed to promastigotes in Novy-Nicolle-Mac Neal (NNN medium supplemented with penicillin (100 U/ml), streptomycin (100  g/ml) and 20% heat-inactivated fetal calf serum (FCS) at 25  C. A fixed initial density of the parasites was transferred to screw-capped vials containing 5 ml of RPMI1640 media to which different concentrations of 2.5, 5, 10 and 20  g asafetida were added and each concentration was done in triplicates. Each run also included control. The mortality of parasitoids was measured by the slide and the enzyme-linked immunosorbent assay (ELISA) methods. RESULTS: After 72 h, asafetida inhibited growth of parasites in all doses in stationary and logarithmic phases. The ELISA measurement suggested that the viability of parasites significantly decreased after 48h (P < 0.05). CONCLUSION: The results show that asafetida could prevent from growth and viability of parasites and this oleo gum resin can be useful for treatment of @entity232 .
