Microscopic quantification of hypercin fluorescence in an orthotopic XXXX @entity150 model after intravesical instillation.

@entity1 @entity1377 @entity121 @entity2735 @entity5 @entity150 @entity35 @entity19486
We have previously investigated the possibility of using @entity19486 as a diagnostic tool for the fluorescence detection of flat @entity150 . In these clinical studies, it was shown that following intravesical application in @entity1 , @entity19486 becomes selectively localized in transitional @entity1377 and @entity2735 ( @entity2735 ). In the present study, we characterized the biodistribution of @entity19486 in @entity35 @entity150 and normal bladder layers after intravesical administration. The biodistribution was evaluated using fluorescence microscopy with computerized image analysis to image and quantify the fluorescence of @entity19486 across the @entity5 and normal bladder wall. The results show that the photosensitizer intravesical administration route provides selective labelling of both the @entity5 and normal urothelium. A @entity19486 dose and instillation time-dependent increase in the @entity19486 fluorescence intensity in both the @entity5 and urothelium was observed, without significant @entity19486 fluorescence in the submucosa and muscle layers. The highest fluorescence ratios in @entity19486 accumulation in the @entity5 and normal urothelium to the @entity121 were achieved at 4 h with 30 micro M @entity19486 (20:1 for the urothelium to @entity121 and 30:1 for the @entity5 to @entity121 ). The difference in fluorescence intensity in @entity5 tissue to the @entity121 following instillation of 8 micro M @entity19486 was 11:1, 25:1 and 28:1 at 1, 2 and 4 h, respectively. The difference in fluorescence intensity in @entity5 tissue to the @entity121 using 30 micro M @entity19486 was 17:1, 27:1 and 31:1 at 1, 2 and 4 h, respectively. The highest absolute fluorescence levels were observed in the @entity5 at 4 h with 30 micro M @entity19486 instillation. The results suggest that under these conditions, PDT with @entity19486 is likely to produce uniform @entity5 eradication, without causing damage to the underlying muscle layers.
