Prestorage white cell reduction in saline-adenine- XXXX -mannitol red cells by use of an integral filter: evaluation of storage values and invivo recovery.

@entity1372 @entity880 @entity972 @entity413 @entity855
BACKGROUND: Prestorage white cell (WBC) reduction in blood components may decrease the incidence of adverse reactions and improve component quality. A bottom-and-top system with an integral third-generation WBC-reduction filter has been studied. STUDY DESIGN AND METHODS: Whole blood was collected from 30 healthy donors: from 20 by using a blood container system with an integral filter and from 10 controls by using a standard blood container system. Ten test units were buffy coat-depleted, stored for 72 hours at 4 degrees C, and then filtered, while an additional 10 test units were buffy coat-depleted and filtered at room temperature within 8 hours of collection. All units were stored at 4 degrees C for 42 days and sampled weekly. RESULTS: The mean WBC content of the 72-hour, 4 degrees C units was 0.33 x 10(6), that of the room-temperature units was 2.6 x 10(6), and that of the buffy coat-depleted controls was 460 x 10(6) (p < 0.0005). No significant differences were found among @entity880 , @entity413 , @entity1372 , @entity972 , and plasma hemoglobin levels in the three groups. @entity855 and 2,3 DPG levels were significantly better preserved in control units than in 72-hour, 4 degrees C units (p = 0.016 and p = 0.032, respectively), but not better than in the room-temperature units. Significant differences were observed between pH values in filtered units and both groups of test units (p = 0.016). In biologic terms however, these differences were small. Red cells from an additional eight healthy volunteer donors were processed by an 8-hour room-temperature method and stored for 35 days. Studies in vivo 24-hour recovery of autologous red cells were performed by transfusing a radiolabeled (51Cr plus 131I-albumin) aliquot after 35 days' storage. Good recovery (mean > 80%) was found by both the single- and double-isotope-label methods. Recovery was significantly greater when calculated by the single-isotope method (p = 0.02). CONCLUSION: The combination of buffy coat removal and filtration in the blood container system with an integral filter achieved effective WBC reduction (> or = 3 log10 reduction from whole blood) without biologically significant detriment to in vitro or in vivo storage values.
