XXXX Level of Human Pancreatic Donors is a Critical Factor for Determination of Islet Efficacy and Survival.

@entity19 @entity1372 @entity3489
UNASSIGNED: Organs from @entity3489 (elevated Na(+)) donors when used for transplantation had dismal outcome. However, islet isolation from hypernatremic donors for both transplantation and research applications has not been investigated yet. A retrospective analysis of in vivo and in vitro islet function studies was performed on islets isolated from hypernatremic (serum @entity1372 levels >=  160 mEq/L) and normal control (serum @entity1372 levels  <= 155 mEq/L) donors. Twelve isolations from 32 hypernatremic and 53 isolations from 222 normal donors were randomly transplanted into diabetic NOD Scid @entity19 . @entity1372 levels upon pancreas procurement were significantly elevated in the @entity3489 group (163.5    0.6 mEq/L) as compared to normal control group (145.9    0.4 mEq/L) (P<0.001). The post-culture islet recovery rate was significantly lower in the @entity3489 (59.1    3.8%) compared to the normal (73.6    1.8%) group (P=0.005). The duration of @entity3489 was inversely correlated with the recovery rate (R2=0.370, P<0.001). Furthermore, the percentage of successful graft function when transplanted into diabetic NOD Scid @entity19 was significantly lower in the @entity3489 (42%) in comparison to the normal control (85%) group (P<0.001). The ability to predict islet graft function post transplantation using donor @entity1372 levels, islet recovery rate, and duration of @entity3489 was significant (P=0.022, 0.036, and 0.042, respectively). In conclusion, duration of donor @entity3489 is associated with reduced islet recovery post culture. The efficacy of islets from @entity3489 donors diminished when transplanted into diabetic NOD Scid @entity19 .
