Lack of evidence for XXXX @entity16325 -mediated neutrophil accumulation in the @entity35 cutaneous microvasculature by @entity130 .

@entity13631 @entity4 @entity16325 @entity3426 @entity13624 @entity35 @entity62525 @entity4756 @entity130
The effect of the non-peptide selective tachykinin @entity16325 antagonist @entity13631 has been investigated on @entity4 formation and neutrophil accumulation induced by @entity130 (50 degrees C for 5 min), mustard oil, substance P, the tachykinin NK1 agonist @entity62525 , and @entity3426 in the abdominal skin of the anaesthetised @entity35 . @entity13631 significantly inhibited (120 nmol/kg i.v.) or prevented (240 nmol/kg i.v.) the early @entity4 formation (0-10 min) induced by @entity130 . However, a dosing strategy which blocked NK1 receptors for 5 h ( @entity13631 , 240 nmol/kg i.v. + 240 nmol/kg s.c.) failed to influence neutrophil accumulation measured 5 h after @entity130 . Thus, the neurogenic component mediated by NK1 receptors is important to elicit the early @entity4 formation, but does not influence subsequent neutrophil accumulation. Topical application of mustard oil (2%), a @entity13624 stimulant, caused @entity16325 -mediated early neurogenic plasma extravasation, but did not induce cutaneous neutrophil accumulation over 5 h. Substance P and @entity62525 at high doses (1 nmol/site) also failed to elicit neutrophil accumulation. Neutrophil accumulation induced by @entity3426 (0.03-3 pmol i.d.) was not affected by @entity13631 pretreatment. In conclusion, despite an early pronounced tachykinin @entity16325 -dependent @entity4 response after @entity130 , the results suggest that subsequent neutrophil accumulation is not mediated by NK1 receptors. Furthermore, we have not obtained any evidence to suggest that either endogenous or exogenous @entity4756 can directly induce neutrophil accumulation in the @entity35 cutaneous microvasculature.
