Myocardial tissue troponins T and I. An immunohistochemical study in experimental models of XXXX .

@entity1 @entity1187 @entity1707 @entity296 @entity1885 @entity63 @entity235 @entity39 @entity1563 @entity6949 @entity406 @entity10020 @entity982 @entity1188 @entity741 @entity35 @entity583
BACKGROUND: Cardiac troponins T ( @entity10020 ) and I ( @entity6949 ) are proven diagnostic and risk stratification biomarkers in @entity1 with @entity1187 . To date, no immunohistochemical studies have been performed which allow visualization of the time course and pattern of myocardial troponin egress from the myocardium during the early evolution of @entity63 in experimental systems. METHODS: We studied archival @entity406 -fixed, @entity982 -embedded myocardium from 50 experimental animals ( @entity235 , @entity1707 and @entity35 ) that had undergone permanent @entity1563 (n = 34) for 0.5-6 h or occlusion of 0.75-6 h followed by reperfusion (n = 16). Histologic sections that included @entity63 and nonischemic myocardium were studied by immunohistochemistry with three different antibodies to @entity1 @entity6949 and one to @entity10020 , using a standard avidin- @entity1885 -peroxidase system. RESULTS: All antibodies detected @entity10020 or @entity6949 in normal myocardium and its loss from @entity39 , in some cases as early as 30 min after @entity1563 , before histologic evidence of @entity39 was present. Loss was nonuniform, being greater at the periphery of the @entity1188 then at their central regions. Usually, loss of @entity10020 appeared greater than loss of @entity6949 . With reperfusion, findings were similar to those after permanent occlusion, except that there was a greater contrast between loss at the periphery compared to the loss in the central region. Considerable residual staining persisted for hours after occlusion, indicating delayed release over time, concordant with sustained serum elevations in @entity1 with @entity583 . No loss of staining was observed in nonnecrotic myocardium. CONCLUSIONS: Immunohistochemical staining using antibodies to @entity1 @entity10020 and @entity6949 can be used to visualize cardiac troponins and document their loss in histologic sections of myocardium in different animal species. Loss of @entity10020 and @entity6949 occurs very early following @entity63 and may precede histologic evidence of @entity39 , but does not occur in myocardium that is not necrotic. Immunohistochemical staining of hearts for @entity10020 and @entity6949 can assist in the often difficult recognition of @entity296 at autopsy, in @entity1 suspected of dying from acute @entity741 .
