The Antitumor Efficacy of XXXX / @entity15787 -Cultured Polyfunctional Neu-Specific T Cells Is @entity310 / @entity4155 Dependent.

@entity1 @entity0 @entity5 @entity19 @entity310 @entity1198 @entity15787 @entity4155 @entity1199 @entity405 @entity1722
PURPOSE: Infusion of @entity1722 -specific T cells, derived from vaccine-primed @entity1 and expanded with @entity1199 /IL12, has induced @entity5 regression in a minority of @entity1 with metastatic treatment-refractory @entity1722 (+) @entity0 . We questioned whether alteration of cytokine growth factors used to culture vaccine-primed T cells could improve antitumor activity. EXPERIMENTAL DESIGN: Using the TgMMTV-neu @entity19 @entity5 model, we cultured T cells derived from @entity19 immunized with a previously defined neu class II peptide, p98-114 (neu p98), and evaluated different cytokine combinations for expansion. RESULTS: Infusion of neu p98-specific T-cell lines derived from all cytokine conditions evaluated resulted in significant antitumor activity compared with infused na  ve splenocytes (P < 0.05). T cells cultured with @entity1199 / @entity15787 could uniquely mediate complete regression of spontaneous @entity5 . @entity1199 / @entity15787 cultured neu-specific T cells demonstrated a different cytokine secretion pattern as compared with other cultured T cells; secreting high levels of @entity310 and @entity4155 (P < 0.05). Moreover, @entity5 -infiltrating @entity405 (+) cells were significantly increased after the infusion of @entity1199 / @entity15787 cultured T cells as compared with @entity5 treated with T cells expanded under other cytokine conditions (P < 0.001). The antitumor effect of the infusion of @entity1199 / @entity15787 cultured cells was mediated by @entity405 T cells. Depletion of @entity310 or @entity4155 , but not IFNy, abrogated the @entity5 growth inhibition induced by the @entity1199 / @entity15787 T cells and markedly decreased the influx of @entity405 into @entity5 . Finally, @entity1199 / @entity15787 -cultured human antigen specific T cells also displayed a similar polyfunctional @entity1198 /Th17 phenotype. CONCLUSIONS: Expansion of @entity1722 vaccine-primed T cells with @entity1199 / @entity15787 may have the potential to effectively mediate @entity5 regression when used in adoptive transfer. Clin Cancer Res; 22(9); 2207-16.   2015 AACR.
