MicroRNA-425-5p regulates chemoresistance in XXXX cells via regulation of @entity32085 .

@entity38485 @entity5 @entity19 @entity14 @entity32085
UNASSIGNED: Acquired chemoresistance represents a major obstacle in @entity5 treatment, the underlying mechanism of which is complex and not well understood. @entity38485 has been reported to be implicated tumorigenesis in a few @entity5 types. However, its role in regulating chemoresistance has not been investigated in @entity14 ( @entity14 ) cells. Microarray analysis was performed in isogenic chemosensitive and chemoresistant HCT116 cell lines to identify differentially expressed miRNAs. miRNA quantitative real-time PCR was used to detect @entity38485 expression levels between drug resistant and parental @entity5 cells. @entity38485 mimic and inhibitor were transfected, followed by CellTiter-Glo(  ) assay to examine drug sensitivity in these two cell lines. Western Blot and luciferase assay were performed to investigate the direct target of @entity38485 . Xenograft @entity19 models were used to examine in vivo function of @entity38485 . Our data showed that expression of @entity38485 was significantly up-regulated in HCT116-R compared with parental HCT116 cells. Inhibition of @entity38485 reversed chemoresistance in HCT116-R cells. @entity32085 ( @entity32085 ) is the direct target of @entity38485 which is required for the regulatory role of @entity38485 in chemoresistance. @entity38485 inhibitor sensitized HCT116-R xenografts to chemo drugs in vivo. Our study demonstrated that @entity38485 regulates chemoresistance of @entity14 cells by modulating @entity32085 expression level both in vitro and in vivo. @entity38485 may represent a new therapeutic target for the intervention of @entity14 .
