Analysis of macrophage presence in XXXX placenta: influence of age and parity status.

@entity19 @entity777 @entity11496
PROBLEM: Beneficial effects of multiparity status have been previously reported by different authors. However, this fact has not been fully explained. Taking into consideration the influence of the parity status on the in vitro asymmetric/protective antibodies and the fact that @entity777 ( @entity777 ) is involved in the production and immune-regulatory functions of placental macrophages, the aim of this work was to compare the placental @entity777 production and tissue macrophage presence in @entity19 with different age and parity status. METHOD OF STUDY: Three groups of @entity19 (CBA/J x CBA/J) were analyzed: primiparous young (PY: 3.0 +/- 0.5 months old), primiparous old (PO: 8.5 +/- 0.5 months old), and multiparous old (MO: 8.5 +/- 0.5 months old, with three to four previous pregnancies). Macrophage and @entity777 were identified in placental tissue by immunohistochemistry employing anti- @entity11496 or anti- @entity777 antibodies. @entity777 secretion was analyzed in the placental culture supernatants by enzyme-linked immunosorbent assay. RESULTS: The results obtained indicate that, despite the level of macrophages observed in the PO placentae was higher than in PY ones, their expression in MO placentae was very much increased, appearing like a thick layer between decidua and trophoblast. However, no significant difference was found among the groups in the tissue expression of @entity777 and in @entity777 secreted in vitro. CONCLUSIONS: Our data indicate that parity status influences the number of local macrophages and might provide evidence that could explain the known beneficial effect of multipaternity. We suggest that the number of previous pregnancies favor the production of a 'protective' population of macrophages.
