Expression of XXXX , and TIMPs 1 and 2 during corneal wound healing.

@entity6930 @entity11018 @entity6929 @entity35 @entity11017 @entity33238
PURPOSE: To determine the expression of @entity33238 and the localization of @entity6930 and @entity11018 during the early stages of corneal wound healing in @entity35 . METHODS. One eye each of 30 adult @entity35 was treated with excimer laser keratectomy; the other eye was untreated. Frozen sections of corneas obtained 6, 18, and 24 hours and 3 and 7 days after wounding were used to perform immunoconfocal microscopy and in situ hybridization. RESULTS: @entity6929 was immunolocalized to the basement membrane zone and superficial stroma, and its mRNA was exclusively localized to basal epithelial cells migrating across the wound at 18 and 24 hours and 3 days after wounding. @entity6930 was also immunolocalized to the basement membrane zone and superficial stroma at the same time points. @entity11017 was immunolocalized to the epithelium and stroma of normal corneas and was predominant in the basal epithelium and superficial stroma at 3 and 7 days after wounding. In situ hybridization confirmed @entity11017 expression by the epithelial cells and the stromal keratocytes. Tissue inhibitor of metalloproteinase-2 was immunolocalized to the epithelium in normal and wounded corneas. Intense @entity11018 labeling of the basement membrane zone was noted 3 days after wounding. CONCLUSIONS: Unlike @entity11017 , @entity6929 is expressed exclusively by migrating basal epithelial cells after wounding. The matrix metalloproteinase-tissue inhibitor of metalloproteinase (MMP)/ @entity6930 systems may play an important role in the early stages of corneal wound healing after excimer laser keratectomy.
