Expression profiling of XXXX basophils: modulation by cytokines and secretagogues.

@entity1 @entity225 @entity5038 @entity56 @entity2772 @entity1199 @entity6889
@entity1 basophils are an accessible @entity1 of the @entity1 @entity56 . There is natural variation in various functional endpoints and in signaling molecule expression but there has been only a limited effort to place this information in the context of mRNA expression profiles. This study examined the hypothesis that unique mRNA signatures could be identified during the response of @entity1 basophils to several known forms of stimulation. Highly purified @entity1 basophils were cultured in vitro and exposed to @entity2772 , @entity225 , NGF, @entity6889 , @entity1199 , anti-IgE Ab, or @entity5038 and the mRNA profiles examined by microarrays. The response to @entity2772 and anti-IgE Ab were examined on 2-3 time frames and the response to @entity2772 examined at several concentrations. In addition, the mRNA signatures of 3 different potential phenotypes were examined. These included basophils with the so-called non-releaser phenotype, and basophils from atopic and non-atopic subjects. Given the role of @entity2772 in basophil maturation and the known profound effects on mature basophil function, it was not surprising that @entity2772 showed the greatest influence on the basophil transcriptome. However, it also became apparent that the act of isolating and culturing basophils was sufficient to induce a large number of changes in the transcriptome, despite high viability and recovery. These "culture-effect" changes dominated the changes in mRNA profiles induced by other stimuli. Unique signatures for anti-IgE antibody and @entity6889 could be identified although the number of gene transcripts (6-30) that were unique to these two stimuli was very limited. There were no apparent unique profiles for @entity225 , NGF, @entity1199 or @entity5038 . Therefore, a potential tool for screening basophil phenotypes was limited to changes that could be induced by @entity2772 ), @entity6889 and anti-IgE Ab.
