Persistence of decidual XXXX cells and @entity9371 genotypes in healthy pregnant and preeclamptic @entity1 : a case-control study in the third trimester of gestation.

@entity1 @entity9371 @entity6460 @entity2940 @entity47661 @entity1468 @entity4071
BACKGROUND: Natural Killer ( @entity47661 ) cells are the most abundant lymphocytes in the decidua during early gestation. The interactions of @entity47661 cells with the extravillous cytotrophoblast have been associated with a normal spiral artery remodeling process, an essential event for a successful pregnancy. Recent data indicate that alterations in the amount of decidual @entity47661 ( @entity47661 ) cells contribute to the development of @entity1468 ( @entity1468 ). Moreover, genetic studies suggest that @entity9371 ( @entity9371 ) expressed in @entity47661 cells influence the susceptibility to @entity1468 . Although @entity47661 cells have been well characterized during early pregnancy, they have been scarcely studied in the third trimester of gestation. The aim of this work was to characterize @entity47661 cells at the last trimester of gestation and to analyze the @entity9371 genotype of healthy and @entity1468 @entity1 . METHODS: Decidual samples were obtained during Caesarean section from control (n = 10) and @entity1468 (n = 9) @entity1 . Flow cytometric analysis of CD3, @entity2940 , @entity4071 and @entity6460 was used to characterize and quantify @entity47661 cells in both groups. Cell surface markers from decidual leukocytes were compared with PBMC from healthy donors. @entity9371 genotyping was performed in genomic DNA (control, n = 86; @entity1468 , n = 90) using PCR-SSP. RESULTS: The results indicate that @entity47661 cells persist throughout pregnancy. They represented 20% of total leukocytes in control and @entity1468 groups, and they expressed the same cell surface markers (CD3-, @entity2940 +, @entity4071 - and @entity6460 +) as @entity47661 in the first trimester of gestation. There were no significant differences in the percentage of @entity47661 cells between control and @entity1468 groups. The analysis of @entity9371 gene frequencies and genotypes was not statistically different between control and @entity1468 groups. The ratio of activating to inhibitory genes indicated that the overall inhibitory balance (0.2-0.5) was more frequent in the @entity1468 group (control, 31.3% vs @entity1468 , 45.5%), and the activating balance (0.6-1.1) was more frequent in the control group (control, 68.6% vs @entity1468 , 54.4%). However this difference was not significant. CONCLUSION: We demonstrated the persistence of @entity47661 cells in @entity1468 and control @entity1 at the third trimester of pregnancy; these @entity47661 cells had a similar phenotype to those found during early pregnancy. The predominance of a @entity9371 inhibitory balance in the @entity1468 group could be associated to the physiopathology of @entity1468 .
