Impact of an autologous oxygenating matrix culture system on XXXX islet transplantation outcome.

@entity32 @entity2308 @entity20382 @entity26 @entity16705 @entity35 @entity4124 @entity2305
Disruption of the pancreatic islet environment combined with the decrease in @entity26 supply that occurs during isolation leads to poor islet survival. The aim of this study was to validate the benefit of using a plasma-based scaffold supplemented with @entity20382 to improve islet transplantation outcome. @entity35 islets were cultured in three conditions: i) control group, ii) plasma based-matrix (P-matrix), and iii) P-matrix supplemented with emulsified perfluorodecalin. After 24  h culture, matrix/cell contacts (Integrinb1, p- @entity2305 / @entity2305 , p- @entity2308 / @entity2308 ), survival ( @entity4124 , TUNEL, FDA/PI), function, and @entity16705 translocation were assessed. Afterwards, P-matrices were dissolved and the islets were intraportally transplanted. Graft function was monitored for 31 days with glycaemia and C-peptide follow up. @entity32 was assessed by histology (macrophage and granulocyte staining) and thrombin/anti-thrombin complex measurement. Islet survival correlated with an increase in integrin, @entity2305 , and @entity2308 activation in P-matrices and function was maintained. @entity20382 supplementation decreased translocation of @entity16705 in the nucleus and post-transplantation islet structure was better preserved in P-matrices, but a quicker activation of IBMIR resulted in early loss of graft function. "Oxygenating" P-matrices provided a real benefit to islet survival and resistance in  vivo. However, intraportal transplantation is not suitable for this kind of culture due to IBMIR; thus, alternative sites must be explored.
