[Expression of cyclooxygenase-2 in XXXX transitional cell @entity150 ].

@entity1 @entity5 @entity150 @entity9859 @entity2247 @entity105 @entity2335
BACKGROUND _ OBJECTIVES: Cyclooxygenase(COX) is a rate-limiting enzyme in prostaglandin synthesis. Recent study suggested that @entity2247 was associated with @entity105 . This study was designed to investigate the role of COX in the development and progression of @entity150 through examining the expression of @entity9859 and @entity2247 in @entity1 @entity150 tissue, normal bladder mucosa, and @entity2335 tissue. METHODS: Reverse transcriptase-polymerase chain reaction(RT-PCR) and immunohistochemistry(Envision method) were applied to detect the mRNA and protein expression of @entity9859 and @entity2247 in @entity150 (BTCC), tissue adjacent to @entity150 , and chronic @entity2335 tissue. Furthermore, the relationship between COX expression level and @entity5 pathologic parameters was analyzed. RESULTS: Using RT-PCR, all 15 frozen specimens from @entity150 were shown to express @entity2247 mRNA, while only 1 of 5 specimens from grossly normal mucosa adjacent to @entity5 expressed @entity2247 mRNA, with significant difference. But @entity9859 mRNA was expressed constitutionally in all of the fresh @entity5 tissue samples. The immunohistochemical result was analogous to RT-PCR result. @entity2247 protein was mainly localized at cytoplasm of malignant cell (positive rate was 50%) but was not expressed in normal bladder mucosa (n = 4) and chronic @entity2335 tissues (n = 5). In contrast, @entity9859 protein was mainly localized at smooth muscle cell of normal tissue and @entity2335 tissue(positive rate was 100%) but was not expressed in @entity5 tissue. In 40 paraffin embedded specimens of BTCC, the intensity of @entity2247 expression was associated with @entity5 grade and stage. The level of @entity2247 expression was higher in grade III @entity5 , than in grade I, II @entity5 , and was higher in invasive @entity5 (T2-T4) than in superficial @entity5 (Ta-T1). CONCLUSIONS: The expression of @entity2247 mRNA and protein was enhanced in @entity1 BTCC, and was associated with the @entity5 , indicating that @entity2247 may play a key role in the development and progression of BTCC.
