Interactions of biapenem with active-site XXXX and metallo-beta-lactamases.

@entity1678 @entity2893 @entity563 @entity5259 @entity23381 @entity526
Biapenem, formerly LJC 10,627 or @entity23381 , a carbapenem antibiotic, was studied in its interactions with 12 beta-lactamases belonging to the four molecular classes proposed by R. P. Ambler (Philos. Trans. R. Soc. Lond. Biol. Sci. 289:321-331, 1980). Kinetic parameters were determined. Biapenem was readily inactivated by metallo-beta-lactamases but behaved as a transient inhibitor of the active-site @entity563 enzymes tested, although with different acylation efficiency values. Class A and class D beta-lactamases were unable to confer in vitro resistance toward this @entity2893 antibiotic. Surprisingly, the same situation was found in the case of class B enzymes from @entity1678 AE036 and @entity5259 5/B/6 when expressed in @entity526 strains.
