Histochemical analyses of steroid hormone receptors in XXXX .

@entity172 @entity1 @entity19097 @entity263 @entity0 @entity1420 @entity7273 @entity5 @entity969 @entity342
Histochemical analyses @entity172 (ER) and @entity1420 ( @entity7273 ) receptors in @entity0 were statistically correlated with results of dextran-coated charcoal (DDC) and @entity969 gradient assays. Correlated for ER was 91% of 363 cases, and for @entity7273 88% of 255 specimens. @entity0 ER/ @entity7273 positivity by histochemistry correlated with a favorable clinical response to endocrine therapies in 72% of 25 cases, while ER/ @entity7273 negativity correlated with a lack of response in 96% of 22 cases with @entity5 . Nuclear ER/ @entity7273 correlated with a poor response to therapy in 8 of 12 @entity1 . An in vitro technique to detect nuclear translocation of ER revealed two groups of ER positive cases, with 11 of 17 exhibiting translocation and 6 not displaying translocation. In @entity263 , 72% of 65 @entity1 were positive for ER and/or @entity342 receptor. Comparison of specimens obtained without and with electrocautery revealed a preponderance of nuclear binding in the latter, suggesting heat-induced nuclear translocation of receptor. @entity19097 , a naturally fluorescent, entirely unaltered @entity172 was also used for histochemical detection of ER. Results correlated with ER by DCC in 87% of 61 @entity0 . @entity19097 was additionally used to visually observe receptor and nuclear translocation of ER in intact whole cells in culture.
