Chemotherapy of visceral leishmaniasis ( XXXX ) in the squirrel monkey (Saimiri sciureus).

@entity3911 @entity281 @entity1559 @entity17004
The relationship of the numbers of amastigotes in the liver to the duration of @entity281 with two lines of a Khartoum strain of @entity3911 [designated the parent (P) line and the @entity17004 ( @entity17004 ) resistant (MAR) line] and the effect of @entity1559 antimoniate on these two lines of @entity3911 were studied in the squirrel monkey. All experimental monkeys were inoculated via the saphenous vein with 32.5 X 10(6) amastigotes (per kg body weight), obtained from heavily-infected hamster spleens. Subsequently in Experiment I, liver biopsy samples were taken chronologically from all monkeys. Imprints of liver were made on glass slides and stained with Giemsa's staining solution, and parasite density per gram of liver tissue was determined. The parasites reached a maximum density of 6.2 X 10(6) amastigotes per gram between two to four weeks and 9.4 X 10(7) amastigotes per gram between four to six weeks in the monkeys receiving the P line and the MAR line, respectively. Parasite numbers then decreased, and all the livers and spleens of all monkeys became microscopically negative for @entity3911 eight to 13 weeks post- @entity281 . Comparison of the multiplication of the two lines of @entity3911 indicated that the MAR line persisted longer in the livers than did the P line. A slight decrease in body weight was observed at eight weeks post- @entity281 . Packed cell volume and haemoglobin were low at four to eight weeks post- @entity281 , but were within the normal range.(ABSTRACT TRUNCATED AT 250 WORDS)
