A Validated High-Throughput Fluorometric Method for Determination of XXXX in Quality Control Laboratory via Charge Transfer Sensitized Fluorescence.

@entity30153 @entity72416 @entity4546
UNASSIGNED: A high-throughput 96-microwell plate fluorometric method was developed and validated to determine @entity4546 ( @entity30153 ) in its dosage forms. The method was based on the charge-transfer (CT) sensitized fluorescence reaction of @entity30153 with @entity72416 (DDQ). This fluorescence reaction provided a new approach for simple, sensitive and selective determinations of @entity30153 in pharmaceutical preparations. In the present method, the fluorescence reaction was carried out in 96-microwell plates as reaction vessels in order to increase the automation of the methodology and the efficiency of its use in quality control laboratories. All factors affecting the fluorescence reaction were carefully studied and the conditions were optimized. The stoichiometry of the fluorescence reaction between @entity30153 and DDQ was determined and the reaction mechanism was suggested. Under the optimum conditions, the linear range was 100-6000  ng/ml with the lowest LOD of 33  ng/ml. Analytical performance of the proposed assay, in terms of accuracy and precision, was statistically validated and the results were satisfactory; RSD was <2.6  % and the accuracy was 98.6-101.6  %. The method was successfully applied to the analysis of @entity30153 in its dosage forms; the recovery values were 98.26-99.60      0.95-2.22  %. The developed methodology may provide a safer, automated and economic tool for the analysis of @entity30153 in quality control laboratories.
