Reemergence of XXXX in southern Japan in 2013.

@entity291 @entity66 @entity548 @entity1282 @entity221
In Japan in 2013, two @entity291 in the northwestern part of Kagoshima Prefecture developed @entity221 and @entity1282 and were suspected of having @entity66 . The @entity548 (EHDV) genome was detected from diseased and asymptomatic @entity291 by reverse transcription-polymerase chain reaction (RT-PCR). High neutralization antibody titers to Ibaraki virus (IBAV) ranging from 1:128 to 1:1,024 were observed in the RT-PCR-positive @entity291 , and the virus was isolated in one of the IBAV-positive farms. A pairwise alignment and phylogenetic analysis based on the major outer coat protein VP2 encoded in segment 2 revealed a close relationship between the isolated viruses and previous IBAV isolates. The phylogeny of VP2 also suggested that an IBAV variant isolated in 1997 was distinct from IBAV and sorted into a heterogeneous serotype, EHDV serotype 7. The findings revealed the reemergence of @entity66 in Japan after a 26-year absence. Interestingly, the co-circulation of EHDV serotype 1 with IBAV was observed in the affected region, suggesting the potential reassortment between two heterogeneous serotypes in the field. Sentinel surveillance in Kagoshima Prefecture indicated that the incursion of IBAV occurred in October 2013 and that its spread was limited within the small area. Inadequate environmental temperatures for vector transmission in late autumn might have limited the virus spread to a wider region. The reemergence of @entity66 showed us the importance of continuous vaccination to prevent economic losses.
