Both acute delivery of and storage with magnesium sulfate promote XXXX and coagulation function.

@entity4612 @entity1423 @entity3736 @entity2400 @entity9495 @entity1760
BACKGROUND: The platelet storage lesion causes loss of function and viability over time. A new paradigm for platelet storage is desired to enable safer, more effective transfusions while reducing waste. We hypothesized that repletion of @entity4612 , which is chelated by @entity3736 anticoagulant, could reduce platelet storage lesion severity when given in conjunction with storage at a refrigerated temperature. METHODS: Apheresis platelet units were collected from healthy donors and stored at 22  C or 4  C. On Days 0, 2, 4, and 8, samples were collected for analyses of receptor-mediated aggregation, coagulation, adhesion to collagen under flow, and viability. In the first series, samples were given an acute dose of @entity2400 before testing; in the second series, storage bags were supplemented with 0-, 3-, or 6-mM @entity2400 . RESULTS: Acutely delivered @entity2400 induced a more rapid coagulation time in apheresis platelets, further enhanced by storage at 4  C. Platelet adhesion to a collagen surface while exposed to arterial shear rates (920 s) was enhanced by @entity2400 supplementation-acute @entity2400 had a large effect on adhesion of fresh platelets, which diminished more rapidly in 22  C samples, while storage with @entity2400 showed significant benefits even out to Day 4 at both temperatures. Although 4  C storage improves the longevity of @entity1423 responses to agonists, @entity2400 supplementation did not change those responses. CONCLUSION: Acute @entity2400 reduces clot time likely through the transient increase of free @entity9495 . Limited differences between platelet function in acute delivery of and storage with @entity2400 diminish the possibility that @entity4612 -induced metabolic inhibition of platelets synergizes with 4  C storage. Regardless, @entity1760 supplementation to platelets is an exciting possibility in transfusion because the adhesion response of 22  C-stored platelets on Day 4 is significantly enhanced when stored with 6-mM @entity2400 .
