Proteins regulated by gonadal steroids in the medial preoptic and ventromedial hypothalamic nucleic of male and female XXXX .

@entity172 @entity230 @entity1420 @entity575 @entity20482 @entity134 @entity35
Protein profiles of brain areas mediating effects of @entity134 hormones on copulation were compared between animals in gonadal @entity134 states predictive of either the presence or absence of copulatory activity. A broad range of proteins present in micropunches of tissue from the medial preoptic area (MPO) and from the ventromedial hypothalamus (VMH) were compared between male and female @entity35 with gonadal @entity230 present or absent. Half of the animals of each gender were gonadectomized 1 month prior to sacrifice. The remaining males were left intact, while the remaining females were gonadectomized, implanted with @entity172 capsules, and injected with @entity1420 prior to sacrifice. These females were screened for sexual receptivity immediately prior to sacrifice. Proteins from the MPO and VMH of each animal were separated by two-dimensional gel electrophoresis, @entity575 stained, and quantified by computerized optical densitometry. Several proteins differed in density between gels of high- @entity134 males and and females and between high- @entity134 and absent- @entity134 animals of one or both genders. Two previously reported sex differences were replicated and found to depend on activational effects of gonadal steroids. Several interesting reversal patterns were noted between MPO and VMH, including three proteins that were affected by gonadectomy in the MPO of males, but not females, and in the VMH of females, but not males, thus correlating with sexual function. These included serum albumin (a possible index of local area blood flow) and @entity20482 , a glycolytic enzyme of anaerobic metabolism. A probable genetic polymorphism was discovered at a locus whose expression appears to be regulated by gonadal steroids.(ABSTRACT TRUNCATED AT 250 WORDS)
