Native XXXX adipose stromal cells: localization, morphology and phenotype.

@entity1 @entity3120 @entity28557 @entity3820 @entity2280 @entity1386
OBJECTIVES: Beside having roles in energy homeostasis and endocrine modulation, @entity1386 ( @entity1386 ) is now considered a promising source of mesenchymal stromal cells (adipose-derived stromal cells or ASCs) for regenerative medicine. Despite numerous studies on cultured ASCs, native @entity1 ASCs are rarely investigated. Indeed, the phenotype of ASCs in their native state, their localization within @entity1386 and comparison with bone marrow-derived mesenchymal stromal cells (BM-MSCs) has been poorly investigated. DESIGN: To address these issues, the stroma vascular fraction (SVF) of @entity1 @entity1386 was extracted and native cell subtypes were isolated by immunoselection to study their clonogenic potential in culture. Immunohistology on samples of @entity1 @entity1386 in combination with reconstruction of confocal sections were performed in order to localize ASCs. RESULTS: Compared with BM-MNCs, all native ASCs were found in the @entity2280 (+) cell fraction of the @entity3120 . Native ASCs expressed classical mesenchymal markers described for BM-MSCs. Interestingly, @entity2280 expression decreased during ASC cell culture and was negatively correlated with cell proliferation rate. Immunohistological analysis revealed that native ASCs exhibited specific morphological features with protrusions. They were found scattered in @entity1386 and did not express in vivo pericytic markers such as @entity28557 , @entity3820 or alpha-smooth muscle actin, which appeared during the culture process. Finally, ASCs spontaneous commitment to adipocytic lineage was enhanced in @entity1386 from obese @entity1 . CONCLUSIONS: The use of complementary methodological approaches to study native @entity1 ASCs revealed their immunophenotype, their specific morphology, their location within @entity1386 and their stemness. Furthermore, our data strongly suggest that @entity1 ASCs participate in adipogenesis during @entity1386 development.
