Effect of long generation times on growth of @entity3404 in XXXX -induced continuous culture.

@entity1 @entity3404 @entity3363 @entity3954 @entity7969 @entity4588 @entity4919 @entity4823 @entity598 @entity1735 @entity8686 @entity505 @entity413
We investigated the ability of @entity3404 , an obligate anaerobe from @entity1 colonic microflora, to grow in a @entity1735 -limited continuous culture at generation times ranging from 3.5 to 28 h per division. Four @entity3363 were tested: @entity413 , @entity3954 , @entity8686 , and @entity4823 . At a generation time of 3.5 h per division, the growth yields for bacteria growing on @entity413 , @entity3954 , and @entity8686 were 76, 68, and 50 g of cells (dry weight) per mol of substrate, respectively. Growth yields at 28 h per division were 61, 52, and 37 g/mol of substrate, respectively. When @entity4823 was the @entity1735 source, a stable population of bacteria was attainable only at generation times longer than 12 h per division. Growth yields at 15 and 32 h per division were 11 and 33 g/mol of substrate, respectively. There was no significant variation with increasing generation times in the specific activities of selected glycolytic enzymes, of disaccharidases such as alpha- and beta-glucosidases and alpha- and beta-galactosidases, or of the polysaccharidase chondroitin sulfate lyase. By contrast, the pattern of fermentation products varied with both the generation time and the @entity505 source. At a generation time of 3.5 h per division, the main products from the fermentation of @entity413 were @entity598 and @entity4588 , with a trace of @entity4919 . At 28 h per division, @entity4919 concentrations were higher and @entity4588 concentrations were lower than at 3.5 h per division. The products from the fermentation of @entity4823 were the same as those from @entity413 fermentation. However, when @entity3954 was fermented, the concentration of @entity598 was much higher at all generation times than when @entity413 was the @entity505 source. When @entity8686 was the @entity505 source, @entity598 was the main fermentation product, and only traces of @entity4919 and @entity4588 were detected. Another characteristic that varied with the growth rate was the ability of @entity3404 to produce the inducible enzyme alpha-glucosidase when exposed to @entity7969 . The ability of the organism to produce this enzyme declined with increasing generation times.
