Expression of colonic antigens by goblet and columnar epithelial cells in XXXX mucosa: their association with inflammatory change and @entity3179 .

@entity1 @entity32 @entity4628 @entity5042 @entity31 @entity3179 @entity1412
AIMS: To investigate colonic metaplasia of goblet and columnar epithelial cells in @entity4628 mucosa; to correlate this with the degree of morphological and inflammatory change; and to assess whether such changes are related to the presence of @entity3179 . METHODS: Biopsy specimens of @entity4628 mucosa were taken from 31 @entity1 (30 with @entity31 , one with @entity1412 ) either before (eight @entity1 ) or after (23 @entity1 ) ileostomy closure. A simple morphological technique was used to assess changes in villous height. Inflammatory change was estimated using an established scoring system for @entity5042 , and acquisition of colonic antigens was determined by immunohistochemistry using three monoclonal antibodies which recognise components of the two major epithelial cell types in the colorectum. The degree of staining with the monoclonal antibodies was graded and the grades correlated with an index of villous atrophy and with the inflammatory scores. RESULTS: Five of eight (63%) pre-closure and 15 of 23 (65%) post-closure biopsy specimens showed increased staining with an antibody against components of columnar epithelial cells. One of eight (12%) pre-closure and 15 of 23 (65%) post-closure biopsy specimens stained with an antibody for colonic mucin. Although both types of staining showed a positive correlation with the @entity5042 score, they also occurred in the absence of @entity32 . CONCLUSIONS: Both goblet and columnar cells acquire colonic characteristics which are incomplete, but may represent a true adaptive response as they can develop in the absence of @entity32 . As the change in goblet cells occurs after ileostomy closure, @entity3179 is likely to be a major contributory factor. Changes in columnar cells may occur before ileostomy closure in the absence of @entity3179 .
