[Establishment of XXXX @entity157 multidrug-resistance cell line (HepG2/Adm) and study apoptosis induced by low-frequency pulse ultrasound exposure].

@entity1 @entity632 @entity5 @entity8993 @entity11092 @entity7895 @entity157
OBJECTIVE: To establish @entity1 @entity157 multidrug-resistance cell line (HepG2/ADM) and to determine the effect of low-frequency pulse ultrasound (US) on MDR cells and investigate its mechanism. METHODS: Using gradual increase of @entity632 (ADM) concentrations in culture, an @entity632 -resistant @entity1 @entity157 cell sub line (HepG2/ADM) was established in vitro. HepG2/ADM cells were cultured in vitro and randomly divided into 4 groups: the control group (HepG2/ADM only), the group ADM by 1.0 mug/ml @entity632 for 1 h, the group US by low-frequency pulse ultrasound for 10 min, and the group US by low-frequency pulse ultrasound and treated with @entity632 simultaneously at same time. A sonication at a frequency of 0.8 MHz, was delivered with an intensity level of 0.5W/cm2, with continuous exposure of 10 min was applied. The ability of US to induce the apoptosis of MDR was evaluated by analyses of fluorescence microscopy, DNA fragmentation assay and flow cytometry assay. RESULTS: HepG2/Adm was resistant to many anti- @entity5 agents, and its IC50 of ADM was 26 times higher than that of parent cell line HepG2. Significant over expressions of @entity7895 , @entity11092 , @entity8993 and GSTs were detected. HepG2/ADM cells radiated by US had the typical characteristics of apoptosis. Compared with the control group (HepG2/ADM, 3.47%); the apoptosis rates were higher in US (12.23%). The therapeutic alliance of US with ADM for MDR cells, have a significant change in the ratio of apoptosis (18.81%, t=1.46 to 5.36, P<0.01). CONCLUSION: HepG2/ADM could have the biological characteristics of @entity1 multidrug-resistance cell line. The US sonication of 0.8 MHz could induce apoptosis of HepG2/ADM cell in vitro, and could act synergistically with @entity632 .
