Standardized analysis of @entity7190 in a XXXX model.

@entity1 @entity154 @entity2194 @entity82 @entity604 @entity7190 @entity35 @entity393
OBJECTIVE: To develop a @entity35 model of @entity7190 (ORN) that uses novel micro-computed tomography bone volume analysis and detailed histology to provide a more effective, quantifiable, and standardized way to study ORN in vivo. STUDY DESIGN: Animal model. SETTING: Academic medical center. SUBJECTS AND METHODS: Modifications to our previously published @entity35 model of mandibular ORN were done to develop an ideal protocol consisting of 10 @entity35 (6 experimental and 4 controls) with their left middle mandibular molar removed 7 days after either 20 Gy high dose rate brachytherapy or sham irradiation. @entity35 were sacrificed 21 days after extraction for landmark defined bone volume and histologic analysis. RESULTS: A standardized method of quantification was achieved in all samples. The radiated group (XRT) had a mean bone volume/total volume (BV/TV) of 13.8% compared to 65.9% for controls (P < .001). There were increases in osteoclasts and @entity82 , @entity393 ,and less bone in radiated samples with a mean (SD)of 5.91 (3.77) @entity2194 ( @entity2194 ) and 4.00(1.83) osteoblasts/ @entity2194 in XRT samples compared to 1.08(1.08) osteoclasts/ @entity2194 and 22.49 (6.00) osteoblasts/ @entity2194 for controls (P <.001). CONCLUSION: Our updated model continues to be clinically analogous to @entity1 mandibular ORN and improves the radiologic and histologic analysis of @entity154 , providing a method for quantification of @entity604 . Further cell-specific staining, including immunohistochemistry, can be used with this model to study potential cellular mechanisms of mandibular ORN and test any future therapeutic options.
