The density of @entity140 receptors activated by a transmitter quantum at the climbing fibre-Purkinje cell synapse in immature XXXX .

@entity35 @entity139 @entity2227 @entity140
We aimed to estimate the number of @entity140 receptors (AMPARs) bound by the quantal transmitter packet, their single-channel conductance and their density in the postsynaptic membrane at cerebellar Purkinje cell synapses. The synaptic and extrasynaptic AMPARs were examined in Purkinje cells in 2- to 4-day-old @entity35 , when they receive synaptic inputs solely from climbing fibres (CFs). Evoked CF EPSCs and whole-cell @entity140 currents displayed roughly linear current-voltage relationships, consistent with the presence of @entity139 subunits in synaptic and extrasynaptic AMPARs. The mean quantal size, estimated from the miniature EPSCs (MEPSCs), was approximately 300 pS. Peak-scaled non-stationary fluctuation analysis of spontaneous EPSCs and MEPSCs gave a weighted-mean synaptic channel conductance of approximately 5 pS (approximately 7 pS when corrected for filtering). By applying non-stationary fluctuation analysis to extrasynaptic currents activated by brief @entity2227 pulses (5 mM), we also obtained a small single-channel conductance estimate for extrasynaptic AMPARs (approximately 11 pS). This approach allowed us to obtain a maximum open probability (Po,max) value for the extrasynaptic receptors (Po,max = 0.72). Directly resolved extrasynaptic channel openings in the continued presence of @entity2227 exhibited clear multiple-conductance levels. The mean area of the postsynaptic density (PSD) of these synapses was 0.074 microm2, measured by reconstructing electron-microscopic (EM) serial sections. Postembedding immunogold labelling by anti- @entity139 /3 antibody revealed that AMPARs are localised in PSDs. From these data and by simulating error factors, we estimate that at least 66 AMPARs are bound by a quantal transmitter packet at CF-Purkinje cell synapses, and the receptors are packed at a minimum density of approximately 900 microm-2 in the postsynaptic membrane.
