Hyaluronic acid grafted with XXXX as a novel peptide formulation.

@entity1391 @entity970 @entity968 @entity625
Hyaluronic acids (HA) grafted with @entity968 ( @entity970 ) ( @entity970 -g-HA) were synthesized. The materials characterization, enzymatic degradability and peptide (insulin) release from solutions of the copolymers were examined. Distribution of bioactive peptides within the polymer chain is well-known for combinations of @entity970 and polysaccharides as aqueous polymer two-phase systems. Insulin was preferentially partitioned into the @entity970 phase in a @entity970 /HA solution system. Enzymatic degradation of the copolymers was strongly dependent on the @entity970 content. Thermal analysis revealed that @entity970 -g-HA exhibited a variation in phase-separated structures depending on the @entity970 content. The solution of @entity970 -g-HA enabled insulin to remain in the @entity970 moieties dispersed in the HA matrix. Leakage of insulin from the copolymers was dependent upon the @entity970 content. Leakage rate of insulin from copolymer containing between 7 and 39% by weight of @entity970 were similar. A dramatic increase in leakage rate occurred when the @entity970 content was increased to greater than 39% by weight. It is considered that the loaded insulin was partitioned into the @entity970 moieties and became entangled with the @entity970 chains. The conformational change of insulin was effectively prevented in @entity970 -g-HA solutions, although insulin was denatured in storage of both @entity1391 buffered solution and HA solution. Such a heterogeneous-structured polymeric solution may be advantageous as an injectable therapeutic formulation for ophthalmic or @entity625 treatment.
