Insights into the Distinguishing Stress-induced @entity137 of Chiral Gold Nanoclusters and the Relationship with XXXX .

@entity2197 @entity137 @entity4191
Chiral gold nanoclusters (Au NCs) exhibit attracting properties owing to their unique physical and chemical properties. Herein we report for the first time chiral gold nanoclusters' @entity137 and potential molecular mechanism. The L-glutathione (i.e. L-GSH) and D-glutathione (i.e. D-GSH)-capped Au NCs were prepared and characterized by HRTEM, UV-vis, photoluminescence and circular dichroism (CD) spectroscopy. Results showed that the CD spectra of L-glutathione (i.e. L-GSH) and D-glutathione (i.e. D-GSH)-capped Au NCs exhibited multiple bands which were identically mirror-imaged, demonstrating that the chirality of @entity2197 -capped NCs had contributions from both the metal core and the ligand. The effects of AuNCs@L- @entity2197 and AuNCs@D-GSH on cells were similar based on the cell physiology related @entity137 , although the effects became more prominent in AuNCs@D-GSH treated cells, including ROS generation, mitochondrial membrane depolarization, cell cycle arrest and apoptosis. Global gene expression and pathway analysis displayed that both AuNCs@L- @entity2197 and AuNCs@D- @entity2197 caused the up-regulation of genes involved in cellular rescue and stress response, while AuNCs@D- @entity2197 individually induced up-regulation of transcripts involved in some metabolic- and biosynthetic-related response. MGC-803 cells were more sensitive to the oxidative stress damage induced by chiral Au NCs than GES-1 cells, which was associated with @entity4191 hypermethylation. In conclusion, chiral gold nanoclusters exhibit this chirality-associated regulation of @entity137 , different gene expression profiling and epigenetic changes should be responsible for observed phenomena. Our study highlights the importance of the interplays between chiral materials and biological system at sub-nano level.
