[Assessment of sentinel nodes in XXXX by rapid peroperative biopsy and immunohistochemistry in serial sectioning].

@entity1 @entity5 @entity0 @entity3
Results of histological examinations of sentinel lymphatic nodes (SLN) obtained by rapid peroperative biopsy (RPB) are presented. Our first experience with 77 @entity1 undergoing localized excision of axillary sentinel nodes is reported. Negative nodes were subsequently examined by means of immunohistochemistry of serial sectioned blocks. The aim of the study was to verify the percentage of identified @entity3 , and thus define the reliability of the RPB. At the same time we tried to determine the ratio of the negative biopsies of SLN, which were found positive subsequent to immunohistochemistry examination of serial sections, to the total amount of peroperative negative findings or a "false negativity". Our results were compared with those recently published. Particular demands and possibilities of the method used are briefly discussed. In the group of 77 @entity1 with @entity0 , the total number of 193 SLN were examined (average 2.5, in a @entity1 ranging from 1 to 7). Out of all examined SLN, 45 @entity1 (58.4%) were negative. @entity3 were identified in 32 @entity1 (41.6%). By rapid preoperative biopsy alone, @entity3 were found in 24%, which represents 75% sensitivity. The specificity was 100%. The following examination of serial sectioned specimens with or without immunohistochemistry showed 8 more @entity1 with @entity3 , which represents the false negativity of 15.1%. The @entity3 found in all 8 @entity1 were small micrometastases, isolated @entity5 cells or clusters of cells.
