Effect of Frozen XXXX Epidermis Storage Duration and Cryoprotectant on Barrier Function Using Two Model Compounds.

@entity1 @entity5834 @entity653 @entity26459 @entity13202
UNASSIGNED: Skin is commonly stored frozen and then thawed prior to use for in vitro permeation experiments. Does frozen storage of skin alter its barrier property? Numerous studies have found contradictory answers to this question. In this study, the steady-state flux and lag time of @entity26459 ( @entity13202 ) were measured for fresh @entity1 skin and skin frozen at -85  C for 1, 2, 3, 6, 9, 12, and 18 months with 10% @entity653 as a cryoprotective agent. No significant differences in steady-state flux were found between fresh and previously frozen samples (p = 0.6). For lag time, a significant (p = 0.002) difference was found among all groups, but comparisons with fresh skin were not significant. Does @entity653 have a cryoprotective effect? The steady-state flux and lag time of @entity13202 and @entity5834 were measured through @entity1 skin stored at -85  C for up to 12 months with and without 10% @entity653 . No significant differences in steady-state flux or lag time were found between samples stored with or without @entity653 for either @entity13202 or @entity5834 (p >=  0.17). These findings support the use of frozen skin to measure the passive permeation of chemicals in studies unconcerned with viability and metabolism. Published by S. Karger AG, Basel.
