[Activation of microglia and astrocytes in different spinal segments after @entity1897 in XXXX ].

@entity1931 @entity9327 @entity19 @entity21846 @entity9271 @entity1897 @entity164 @entity9 @entity130
Spinal microglia and astrocytes play an important role in mediating behavioral hypersensitive state following @entity1897 . However, little is known about the expression patterns of activated microglia and astrocytes in the spinal dorsal horn. The aim of the present study was to investigate the spatial distribution of microglial and astrocytic activation in cervical, thoracic, lumbar and sacral segments of spinal dorsal horn following chronic @entity130 . The hind paw withdrawal threshold (PWT) of @entity164 ( @entity164 ), @entity21846 (YFP) and @entity9327 (YFP) @entity19 to mechanical stimulation was determined by von Frey test. Immunofluorescence staining was used to examine the spatial distribution of microglial and astrocytic activation in the spinal dorsal horn. Following CCI, all the @entity164 , @entity21846 (YFP) and @entity9327 (YFP) @entity19 developed robust @entity1931 in the ipsilateral paw on day 3 after CCI, and the @entity1931 was observed to last for 14 days. In comparison with sham groups, the PWTs of CCI group animals were significantly decreased (P < 0.01, n = 6). On day 14 after CCI, @entity21846 (YFP)-GFP immunofluorescence intensity was significantly increased in the ipsilateral lumbar spinal dorsal horn of the @entity21846 (YFP) @entity19 (P < 0.01, n = 6), but no detectable changes were observed in other spinal segments. Increased @entity9327 (YFP)-GFP immunofluorescence intensity was observed in the ipsilateral thoracic, lumbar and sacral spinal segments of the @entity9327 (YFP) @entity19 on day 14 after CCI. @entity9271 and @entity9327 immunofluorescence staining in @entity164 @entity19 showed the same result of microglia and astrocyte activation on day 14 after CCI. @entity21846 (YFP)-GFP and @entity9327 (YFP)-GFP immunofluorescence signal was colocalized with microglial marker @entity9271 and astrocytic marker @entity9327 , respectively. Interestingly, on day 3 after CCI, @entity9271 -immunoreactivity was significantly increased in the ipsilateral thoracic, lumbar and sacral spinal segments of @entity164 @entity19 , whereas the significant upregulation of @entity9327 -immunoreactivity restrictedly occurred in the ipsilateral lumbar spinal segment. These results suggest that microglial and astrocytic activation may be involved in the development and maintenance of secondary @entity1931 in @entity19 with @entity9 .
