XXXX supplementation to semen extender improves the quality of frozen-thawed @entity235 spermatozoa for transcervical insemination.

@entity1568 @entity235 @entity1792 @entity2041 @entity1132 @entity6172 @entity653 @entity9592
The present study was conducted to evaluate whether supplementation of semen extender with @entity2041 ( @entity6172 ) can maintain the quality of frozen-thawed @entity235 spermatozoa. Eighteen ejaculates were obtained from 5 @entity235 and placed in extender (20% egg yolk, Tris, @entity1792 , @entity1132 , @entity9592 , antibiotics and 6.5% @entity653 ) containing 0 (control), 2.5, 5, 7.5 or 10 mM @entity6172 . The samples were cooled to 4 C and then frozen in liquid @entity1568 vapor. Motility parameters of the sperm were evaluated at 0, 1, 2, 3, 4, 12 and 24 h after thawing. Sperm motility was higher in the 5 mM @entity6172 group than in the control or 2.5 and 10 mM @entity6172 groups; this effect was observed at 1 to 24 h after thawing (P < 0.05). The 5 mM @entity6172 group had a higher sperm viability index at 12 and 24 h after thawing compared with the other groups (P < 0.05). Acrosome integrity, evaluated at 4 h after thawing, was greater in two of the @entity6172 -treated groups (5 and 10 mM) compared with the control. Lipid peroxidation (LP) levels immediately after thawing were lower in the 5 and 10 mM @entity6172 groups compared with the control, while those at 12 h after thawing did not differ significantly. Frozen-thawed semen in the 5 mM @entity6172 group was used for transcervical insemination of 4 bitches, resulting in delivery of 5 puppies from 2 bitches. These results indicate that supplementation of semen extender with 5 mM @entity6172 was effective in improving motility, longevity and acrosomal integrity and inhibiting LP levels in post-thaw @entity235 spermatozoa, without any adverse impacts on full-term development after transcervical insemination.
