Fluorometric determination of @entity72288 and other @entity67848 by precolumn derivatization with XXXX and column liquid chromatography.

@entity67848 @entity28945 @entity32738 @entity95 @entity72288 @entity20662 @entity10009
The increasing therapeutic use of @entity32738 metal binding agents, such as @entity72288 ( @entity20662 ), has stimulated the need for a sensitive and selective method for their determination in biological fluids. A method has now been developed in which @entity20662 was converted to a highly fluorescent and stable derivative by reaction with @entity28945 in aqueous solution at pH 8.3. The reaction was complete within 5 min. The derivative was separated by ion-pair reversed-phase column liquid chromatography. The mass spectrum of the derivative showed that two @entity28945 molecules reacted with one @entity20662 molecule. This method is also applicable to the determination of other @entity67848 . The detection limit for @entity20662 in urine is 10 pmol per 20-microliters injection and the precision is 7.4% at the 100-pmol level. The fluorescence response was linear from 1 to 400 micron. This method was used to determine @entity20662 in the urine of @entity95 treated with this metal binding agent. In addition, total @entity20662 was determined by adding @entity10009 to the same urine to reduce biotransformed and oxidized @entity20662 .
