Involvement of XXXX in mitochondrial apoptosis by @entity7227 under ambient UV radiation.

@entity1 @entity7227 @entity4674 @entity467 @entity21184 @entity57479 @entity5353 @entity98 @entity10155
@entity7227 ( @entity10155 ), a derivative of @entity57479 has been most commonly used as an ingredient of oxidative hair dye and permanent tattoos. We have studied the phototoxic potential of @entity10155 under ambient ultraviolet radiation. @entity10155 is photodegraded and form a novel photoproduct under UV A exposure. @entity10155 shows a concentration dependent decrease in cell viability of @entity1 Keratinocyte cells (HaCaT) through @entity98 and NRU test. Significant intracellular ROS generation was measured by @entity21184 assay. It caused an oxidative DNA damage via single stranded DNA breaks, micronuclei and CPD formation. Both lysosome and mitochondria is main target for @entity10155 induced apoptosis which was proved through lysosomal destabilization and release of @entity467 by immunofluorescence, real time PCR and western blot analysis. @entity467 process @entity5353 to active tBID which induces the release of @entity4674 from mitochondria. Mitochondrial depolarization was reported through transmission electron microscopy. The cathepsin inhibitor reduced the release of @entity4674 in @entity10155 treated cells. Thus study suggests that @entity10155 leads to apoptosis via the involvement of lysosome and mitochondria both under ambient UV radiation. Therefore, photosensitizing nature of hair dye ingredients should be tested before coming to market as a cosmetic product for the safety of @entity1 beings.
