Isolation and in vitro growth of XXXX -infiltrating lymphocytes, and an analysis of their surface phenotypes.

@entity1 @entity3647 @entity404 @entity5 @entity11 @entity816 @entity4071 @entity1199 @entity405
The present investigation was conducted in order to examine the feasibility of isolating and growing @entity11 -infiltrating lymphocytes in vitro as possible effector cells for use in new adoptive immunotherapy. Eight surgical specimens obtained from @entity1 with malignant @entity816 were treated by enzyme dispersion; the cells were separated on a density gradient and grown in the presence of @entity1 recombinant @entity1199 . The cultured lymphocytes were tested for cell-surface markers by using monoclonal antibodies in a flow cytometric analysis. In all cases the @entity11 -derived lymphocytes were grown in culture for several weeks with substantial increases in cell numbers (at least 5 X 10(8) cells). The mature T cell population (CD3, 89%) was found to have an increased proportion of the cytotoxic/suppressor phenotype @entity405 (55%) as compared to peripheral blood lymphocytes (PBL's). Eighty-six percent of the cultivated lymphocytes expressed HLA-DR. The @entity3647 was predominantly expressed on the helper subset ( @entity404 -positive). Otherwise, anti- @entity4071 , which specifically reacts with natural killer (NK) cells, did not stain significantly more of the cultured gliomaderived lymphocytes compared with lymphocyte-activated PBL's. These results corroborate the observations made with conventional immunohistochemical examination. It has been demonstrated that T lymphocytes isolated from @entity1 @entity5 are enriched for specific reactivity to their autochthonous @entity5 cells. These experiments support the possible use of @entity11 -infiltrating lymphocytes as a new treatment for @entity1 with @entity11 .
