Value of PCR detection of XXXX gamma gene rearrangement in the diagnosis of @entity2617 .

@entity4888 @entity32 @entity2617 @entity784 @entity20956 @entity6057 @entity14041 @entity8548
In this study, we analyzed the reliability and usefulness of the polymerase chain reaction (PCR) for the detection of T-cell receptor ( @entity6057 )gamma gene monoclonal rearrangement. We first tested for the specificity and sensitivity of this strategy, against the classical criteria of Southern blot analysis (SBA). Of the 27 samples tested, results agreed in all but two. Broader analysis of these cases demonstrated the high specificity (absence of false positives) of the PCR strategy, together with its limited sensitivity (10% of false negatives). The usefulness of this PCR approach was then tested on a panel of 28 biopsy specimens of @entity2617 . Monoclonal @entity6057 gamma rearrangement was detected in seven of eight cases of early stage @entity4888 ( @entity4888 ), one of two @entity14041 ( @entity14041 ) cases, two of two non- @entity4888 @entity784 , and two of three @entity8548 . Monoclonality was not detected in any of the 11 benign cases ( @entity20956 and @entity32 ). Results obtained with this new molecular strategy provide additional support for the hypothesis of a monoclonal origin for most early stage T-cell @entity4888 . They also suggest the heterogeneous nature of some @entity8548 . Therefore, due to the difficulty in detecting T-cell monoclonality by immunohistochemical techniques, PCR can be a useful alternative strategy to SBA. It could also be used as a complementary technique in the routine diagnosis of T-cell cutaneous infiltrates.
