XXXX -responsive @entity3078 -related factor, @entity73050 , promotes osteoblast differentiation and interacts with the bone morphogenetic protein- @entity2447 pathway.

@entity10173 @entity14901 @entity2447 @entity19 @entity641 @entity2450 @entity21602 @entity73050 @entity8385 @entity3078 @entity4286 @entity8837
The mechanisms whereby the @entity10173 ( @entity10173 ) exerts its anabolic action on bone are incompletely understood. We previously showed that inhibition of @entity8837 enhanced @entity3078 -induced bone anabolic action in osteoblasts. These findings suggested the hypothesis that changes in gene expression associated with the altered @entity3078 -induced signaling brought about by an @entity8837 inhibitor would identify novel bone anabolic factors in osteoblasts. We therefore performed a comparative DNA microarray analysis between empty vector-transfected @entity19 osteoblastic @entity19 cells and @entity4286 -treated stable @entity3078 -overexpressing @entity19 cells. Among the novel factors, @entity73050 was selected on the basis of its rapid induction by @entity10173 independent of later increases in endogenous @entity641 . The levels of @entity73050 increased with time in cultures of @entity19 cells and @entity19 mesenchymal @entity19 cells committed to the osteoblast lineage by @entity8385 . @entity10173 stimulated @entity73050 levels within 1 h as determined by Western blot analysis and immunocytochemistry in @entity19 cells. @entity19 cells stably overexpressing @entity73050 exhibited elevated levels of @entity2447 , @entity2450 , alkaline phosphatase, and b-catenin, whereas @entity73050 augmented @entity8385 -induced @entity2447 levels in mesenchymal cells. @entity73050 interacted with @entity2447 , @entity14901 , and @entity21602 in C2C12 cells. In conclusion, we identified a @entity3078 -related factor, @entity73050 , that is induced by @entity10173 and promotes differentiation in @entity19 osteoblastic cells. @entity73050 is an important molecule in the pathway downstream of @entity10173 and @entity3078 signaling in osteoblasts.
