Application of polymerase chain reaction and subsequent phylogenetic analysis to the diagnosis of XXXX in the central nervous system.

@entity1 @entity4767 @entity410 @entity5827 @entity281
BACKGROUND: @entity4767 (CNS) are often difficult to diagnose, even if consistent conventional laboratory methodologies are used. OBJECTIVES: To clarify the efficiency of two polymerase chain reaction (PCR) methods for the sensitive detection of enteroviruses and for the identification of enteroviral genotypes based on phylogenetic analysis of the amplified genome sequences, and to facilitate the diagnosis of @entity281 in CNS. STUDY DESIGN: Cerebrospinal fluid (CSF), throat swab, rectal swab, and/or serum samples were collected from 171 @entity1 with @entity5827 and 67 @entity1 with @entity410 . The samples were tested for the presence of enteroviruses by cell culture and PCR methods for the detection and identification of enteroviruses. RESULTS: In 111 (64.9%) of 171 @entity1 with @entity5827 , enteroviruses were isolated by cell cultures from any site. In 143 (83.6%) @entity1 , including 110 of 111 @entity1 with @entity5827 , the enteroviral genome was detected in CSF by PCR. No enterovirus was isolated from any site for the 67 @entity1 with @entity410 . PCR detected the enteroviral genome in CSF samples from 13 (61.9%) of 21 @entity1 who developed @entity410 in the summer (June-August). Phylogenetic analysis of amplified genome sequences showed that the major pathogens of @entity410 in summer were group A coxsackieviruses, which are usually difficult to isolate by cell culture. CONCLUSION: PCR methods for the detection and identification of enteroviruses were useful for the diagnosis of @entity281 in CNS.
