Effects of @entity11838 , a marker of oxidative stress, on the @entity3840 of XXXX placenta in @entity5870 .

@entity1 @entity3152 @entity1421 @entity26 @entity5870 @entity1110 @entity13789 @entity3840 @entity11838
@entity5870 ( @entity5870 ) is one of the causes of @entity3152 . A recent study has indicated that @entity1110 oxidase, a reactive @entity26 species (ROS)-producing enzyme, is activated in @entity5870 . @entity5870 is thought to be closely associated with oxidative stress. We have hypothesized that oxidative stress in @entity5870 may induce @entity3152 . The purpose of this study is to examine the effect of @entity11838 ( @entity13789 ), which is a marker of oxidative stress, on @entity1 placenta during @entity3152 . We initially examined the @entity13789 -modified proteins in @entity1 placentas by immunoblotting and immunohistochemistry using anti- @entity13789 antibody. To examine the effect of @entity13789 on @entity1 placenta, we stimulated @entity1 placental tissue with @entity13789 . The expressions of @entity3840 ( @entity3840 ) mRNA and protein were observed by RT-PCR and western blot analysis respectively. Furthermore, we measured the peroxidase activity of @entity3840 by COX activity assay kit. @entity1421 ( @entity1421 ) in the supernatants of placental tissue was also determined by enzyme-linked immunosorbent assay. Immunoblotting and immunohistochemistry showed that the levels of @entity13789 -modified proteins were increased in the placentas with @entity5870 , compared to the normal placenta. @entity13789 induced the expression of @entity3840 mRNA, protein and activity in the placental tissue culture stimulated with @entity13789 . In addition, @entity1421 was also released into the medium in a time-dependent fashion. These findings suggest that @entity13789 -modified proteins, which were increased in the placenta with @entity5870 , play an important role in @entity3152 .
