Distribution of three antituberculous drugs and their metabolites in different parts of pathological vertebrae with XXXX .

@entity1 @entity5585 @entity3083 @entity3808 @entity3109 @entity1215 @entity378
STUDY DESIGN: To detect drug concentration levels and metabolite using high-performance liquid chromatography. OBJECTIVE: To map concentration levels of three antituberculous drugs and two metabolites in the abnormal osseous tissues around the foci of @entity1 with @entity3083 . SUMMARY OF BACKGROUND DATA: Concentration levels of antituberculous drugs in the focus of @entity3083 has been reported. However, the mapping of drugs distribution in different regions surrounding the foci of @entity378 vertebrae remains unexplored, as well as the metabolite of the drugs. METHODS: Thirty-eight @entity1 with @entity3083 were assigned into @entity3808 group (n = 13) and nonsclerotic group (n = 25) based on computed tomographic (CT) images. All @entity1 received a chemotherapy 10 months with 2HRZE/8H(2)R(2)E(2). All @entity1 received surgery after 4 weeks of chemotherapy. Samples of serum, ilium, and pathologic vertebral tissues, including the foci, @entity3808 wall (if applicable), region I of abnormal osseous tissues (within 4 mm), and region II of abnormal osseous tissues (more than 4 mm) from the foci were collected during operation. Concentration levels and metabolite of three drugs were measured using high-performance liquid method for all samples. Differences of means within groups were evaluated by ANOVA and Dunnett post hoc. A significant levels was set at P < 0.05. RESULTS: Concentration levels of drugs varied greatly in different regions of @entity3083 vertebrae. Concentration levels of @entity3109 and @entity1215 were higher than the effective bactericidal concentration (EBC) level and that of @entity5585 was five times of the minimal inhibitory concentration in the region II of abnormal osseous tissues and the ilium of nonsclerotic group. Three drugs achieved EBC in the region I of abnormal osseous tissues in the nonsclerotic group but not in the @entity3808 group. Except @entity5585 no drugs and their metabolite were identified in the foci of the @entity3808 group, whereas there is trace of drugs and their metabolite in the foci of the nonsclerotic group. CONCLUSION: Three drugs resulted in an effective bactericidal concentration level in osseous tissues around the foci of @entity3083 except the osseous tissues 4 mm surrounding the @entity3808 wall. The results suggested that osseous tissues within 4 mm surrounding the @entity3808 wall should be excised during the surgery.
