Separation and characterization of the neoplastic and stromal elements of the R3230AC mammary XXXX .

@entity5 @entity1324 @entity420 @entity35 @entity1068
The purification of the various types of cells in solid @entity5 is necessary for study of their biochemical and immunological functions. The R3230AC @entity420 contains both a variety of stromal cells and well-differentiated neoplastic cells that possess many of the biosynthetic capabilities of normal @entity35 mammary cells. Suspensions of cells from @entity5 weighing less than 1.2 g consisted of 70.4 +/- 7.2% (S.D.) malignant cells by morphology, 6.8 +/- 3.0% lymphocytes, 6.3 +/- 3.2% macrophages, 15.2 +/- 5.2% red blood cells, 0.8 +/- 0.5% granulocytes, and 0.5 +/- 0.6% unidentified cells. Sedimentation of the cells from the R3230AC @entity5 in a previously described isokinetic gradient resulted in a 5- to 6-fold purification of lymphocytes and macrophages. The modal fraction of malignant cells contained 95.3 +/- 2.9% malignant cells. Detection of alpha-lactalbumin by the direct peroxidase conjugate technique gave vacuolar staining of malignant cells, in contrast to the indirect and peroxidase-antiperoxidase techniques which stained ducts from normal lactating mammary gland and a wide variety of cells without vacuoles in the @entity5 . The best fixative for frozen sections, paraffin-embedded tissue, and cell suspensions was 50% @entity1068 -50% @entity1324 . The suspensions of @entity5 cells contained 14.4 +/- 9.4% cells with histochemically demonstrable alpha-lactalbumin. Squamous metaplasia was commonly observed in @entity5 than lactating @entity35 .
