Significant association of Rho/ROCK pathway with invasion and XXXX of @entity150 .

@entity1 @entity33760 @entity5 @entity3547 @entity16505 @entity150 @entity3 @entity563 @entity1889
PURPOSE: The small GTP-binding protein Rho and its best-characterized downstream effector Rho-associated @entity563 - @entity1889 protein kinase, ROCK, participate in actin cytoskeleton organization, and are linked to pathogenesis and progression of several @entity1 @entity5 . We investigated the roles of Rho and ROCK in @entity150 . EXPERIMENTAL DESIGN: Using Western blotting, we quantitated Rho and ROCK protein expression in paired @entity5 and nontumor surgical samples from 107 consecutive Japanese @entity1 with @entity150 . RESULTS: @entity3547 , @entity33760 , and ROCK were more abundant in @entity5 and metastatic lymph nodes than in nontumor bladder and uninvolved lymph nodes (P < 0.0001). Amounts of @entity3547 and @entity33760 protein, and ROCK protein expression correlated positively with one another (P < 0.0001). High @entity3547 , @entity33760 , and ROCK expression were related to poor @entity5 differentiation (P < 0.05, P < 0.01, and P < 0.01, respectively), muscle invasion (P < 0.001), and lymph node @entity3 (P < 0.05). Kaplan-Meier plots linked high @entity3547 , @entity33760 , and ROCK protein expression to shortened disease-free and overall survival (P < 0.0001). By univariate analysis, high @entity3547 , @entity33760 , and ROCK protein expression predicted shortened disease-free and overall survival (P < 0.0001). By multivariate analysis, only @entity33760 was independently influenced in disease-free survival (P < 0.05), and @entity3547 and @entity33760 in overall survival (P < 0.001). In contrast, @entity16505 expression was inversely related to the grade and stage (P < 0.05), and its higher expression is associated with better overall survival (P < 0.05). In superficial @entity5 (Ta or T1; 63 @entity1 ), @entity3547 , @entity33760 , and ROCK were unrelated with recurrence-free survival. Overall survival in @entity5 invading muscle (T2 to T4; 44 @entity1 ) was significantly influenced by @entity3547 , @entity33760 , and ROCK in a Kaplan-Meier analysis (P < 0.0001, P < 0.0001, and P < 0.01, respectively). Whereas @entity3547 , @entity33760 , and ROCK independently predicted shortened overall survival in @entity1 with invasive @entity5 by univariate analysis (P < 0.0001, P < 0.0001, and P < 0.01, respectively), only @entity33760 did so by multivariate analysis (P < 0.05). CONCLUSION: Rho/ROCK pathway apparently involved in occurrence and progression of @entity150 may be valuable prognostic markers.
