Locus-specific changes in cell wall composition characteristic of osmotic mutants of XXXX .

@entity73300 @entity1522 @entity11485 @entity4823 @entity1980 @entity413 @entity3363
The osmotic phenotype of @entity1980 is characterized by inhibition of growth at high osmolalities of growth medium. Mutations at six osmotic loci of linkage group I were examined to assess the biochemical and physiological effects of these mutants. Isolated cell walls from 23 osmotic strains were compared with the wild type with regard to quantitative levels of the following components: percentage of total dry weight, total @entity413 , alkali-soluble @entity413 , nonglucose @entity3363 , @entity1522 , @entity4823 , @entity11485 , and a compound tentatively identified as @entity73300 . The last component has not previously been observed in @entity1980 cell walls. Although the cell wall dry weight content of osmotic mutants was not altered, walls isolated from all of the osmotic strains had less alkali-insoluble @entity413 than those from the wild type. In addition, all of the loci except cut exhibited other consistent differences from the wild type. The os-1, os-3, and os-5 mutants had low levels of alkali-soluble @entity413 . The os-3 and os-5 mutants had high levels of nonglucose @entity3363 , and flm-2 had a low level of nonglucose @entity3363 . The os-4 mutants had low levels of @entity11485 and @entity1522 and high levels alkali-soluble @entity413 . An os-1 mutant, B135, produced less of the whole alkali-soluble fraction of the cell wall.
