Improved PCR assay for the species-specific identification and quantitation of XXXX in water.

@entity71279 @entity2114 @entity2115
UNASSIGNED: @entity2115 outbreak is a major global health care problem. However, current @entity71279 risk assessments may be compromised by uncertainties in @entity71279 detection methods, infectious dose, and strain infectivity. These limitations may place public health at significant risk, leading to significant monetary losses in health care. However, there are still unmet needs for its rapid identification and monitoring of legionellae in water systems. Therefore, in the present study, a primer set was designed based on a LysR-type transcriptional regulator (LTTR) family protein gene of @entity71279 because it was found that this gene is structurally diverse among species through BLAST searches. The specificity of the primer set was evaluated using genomic DNA from 6 strains of @entity2114 , 5 type strains of other related @entity71279 species, and other 29 reference pathogenic bacteria. The primer set used in the PCR assay amplified a 264-bp product for only targeted six strains of @entity2114 . The assay was also able to detect at least 1.39   *   10(3) copies/ l of cloned amplified target DNA using purified DNA or 7.4   *   10(0) colony-forming unit per reaction when using calibrated cell suspension. In addition, the sensitivity and specificity of this assay were confirmed by successful detection of @entity2114 in environmental water samples.
