Independent and synergistic effects of @entity6026 and interleukin-12 in augmenting cytotoxic T lymphocyte responses and XXXX production in aging.

@entity19 @entity137 @entity60 @entity6026
Aged @entity19 exhibit diminished CD8(+) cytotoxic T lymphocyte (CTL) response to influenza virus. Previously, interleukin-12 (IL-12) was shown to partially restore in vitro influenza virus-specific CD8(+) CTL activity and @entity60 ( @entity60 ) production in aged @entity19 . The present study investigated @entity6026 production and its ability to collaborate with IL-12 to enhance these responses to the levels of young @entity19 . @entity6026 protein production and mRNA expression in influenza virus-specific CTL from aged @entity19 were higher than from young @entity19 . In contrast, @entity6026 receptor (IL-18R) mRNA expression was significantly reduced in CD8(+) CTL from aged @entity19 . Generation of CTL in the presence of IL-12 alone caused a significant increase in @entity60 production in both old and young @entity19 . @entity6026 treatment alone significantly increased @entity60 in CTL from young but not old @entity19 . However, a combination of @entity6026 and IL-12 significantly increased @entity60 in both old and young @entity19 . @entity6026 and IL-12, either alone or in combination, stimulated significant influenza virus-specific @entity137 in both old and young @entity19 , but no significant synergistic effect was observed. These results represent an initial demonstration of downregulated IL-18R expression in aging @entity19 and are consistent with age-related @entity137 . Potentially, @entity6026 and IL-12 can augment @entity60 production and reverse CD8(+) @entity137 in aging, independently or synergistically.
