Purging and haemopoietic progenitor cell selection by XXXX + cell separation.

@entity1 @entity1261 @entity0 @entity1885 @entity5 @entity2280
@entity5 cell contamination of autologous peripheral blood stem cell samples (PBSC) and bone marrow (BM) is frequent. Enrichment of @entity2280 + stem cells is a promising approach to purging @entity5 cells from autografts without damaging progenitor cells. @entity0 cells were seeded (10(-3)-10(-7)) into mononuclear cells from @entity1261 -mobilised PBSC and BM harvests from @entity1 without @entity0 . @entity2280 + cells were enriched from mixtures either by immunomagnetic separation (Isolex-50, and MiniMACS) or by @entity1885 -streptavidin immunoaffinity columns (Ceprate-LC). @entity2280 + cell fractions were determined by FACS, @entity5 cells were detected immunocytochemically with an anti-pancytokeratin antibody. The @entity2280 + cells were enriched with a median purity of 92.2% (43.5-96.1) (n = 17) (Isolex-50), 96.5% (66.6-99.2) (n = 17) (MiniMACS) and 77.9% (31.4-93.6) (n = 15) (Ceprate-LC) from PBSC and BM harvests. The percentages of median recovery of @entity2280 + cells were 30.8% (18.6-71.8) (Isolex-50), 69.9% (39.1-100) (MiniMACS) and 42.9% (23.7-100) (Ceprate-LC). The median @entity5 cell reductions in log steps were 3.7 (2.9-4.3) (n = 13) (Isolex-50), 3.5 (2.6-4.3) (n = 13) (MiniMACS) and 1.5 (0.9-2.9) (n = 17) (Ceprate-LC). Results were compared statistically by univariate analysis. Purity was significantly (P < 0.05) better after MiniMACS selection. Recovery rates were significantly different between all devices tested. @entity5 cell purging was superior after immunomagnetic separation (P < 0.001). @entity5 cell purging is a main objective of @entity2280 + selection in the autologous setting. Our in vitro data clearly indicate that immunomagnetic separation is more efficient in the prevention of accidental reinfusion of contaminating @entity5 cells compared to immunoaffinity. However, it is not yet known if the same results can be obtained with fresh contaminating @entity5 cells.
