Autoantibodies against bromelainized XXXX erythrocyte: strain distribution of serum idiotype expression and relative peritoneal cell activity.

@entity19 @entity742 @entity2723 @entity6872
Previously, we demonstrated that the naturally occurring @entity19 autoantibodies directed against bromelainized @entity19 red blood cells (BrMRBC) comprised a family of structurally related molecules bearing a common idiotypic determinant ( @entity2723 ) based on structural and idiotypic analysis of a series of anti-BrMRBC monoclonal autoantibodies derived from a fusion of peritoneal cells (PerC) with @entity6872 . In the present studies, we have evaluated the quantitative expression of circulating @entity2723 idiotype related to autoantibodies against BrMRBC in relation to specific PerC anti-BrMRBC plaque-forming activity in an individual @entity19 of different strains. The data presented here show no direct relationship between serum @entity2723 idiotype expression and PerC anti-BrMRBC plaque-forming activity in an individual @entity19 of all strains tested. However, the circulating @entity2723 idiotype content is higher in strains, viz., CBA/J, NZB, C3H, BXSB, and Biozzi high responder (H) @entity19 which exhibit a high perC autoantibody secretory activity against BrMRBC. The strains such as BALB/c, DBA2, SJL/J, CBA/N, and Biozzi low responder (L) express little or no circulating @entity2723 idiotype with a corresponding small or no PerC anti-BrMRBC activity. Furthermore, the PerC "auto"-immune phenomenon is markedly expressed in the normal CBA/J strain since these @entity19 show a higher percentage ratio of @entity2723 idiotype over serum IgM (2.68%) as well as highest PerC anti-BrMRBC plaque-forming activity (11,319 +/- 18,029 plaques per million viable cells) compared to other normal and autoimmune strains tested. Nevertheless, the highest circulating serum @entity2723 idiotype (49.4 micrograms/ml) is observed in the autoimmune NZB @entity19 . The immunodeficient CBA/ @entity19 fail to express detectable levels of @entity2723 idiotype in their serum. The experiments conducted in genetically selected outbred Biozzi (H and L) strain have revealed remarkable differences in serum @entity2723 idiotype expression as well as PerC anti-BrMRBC plaque-forming activity in these two lines. The expression of @entity19 PerC "auto"-immune phenomenon and quantitative circulation of @entity2723 idiotype in the serum seem to be related to regulatory mechanisms as for @entity742 erythrocytes and other natural antigens earlier demonstrated to be under polygenic regulation in Biozzi (H and L) @entity19 .
