Regulation of apoptosis and autophagy by XXXX in vascular endothelial cells.

@entity4993 @entity2434 @entity876 @entity540 @entity4291
@entity4993 (SPC), an important cardiovascular mediator derived from @entity4291 that has atheroprotective effects via actions on vascular endothelial cells (VECs) at normal levels in vivo. However, the underlying mechanism is not well known. To clarify this question, we first investigated the effect of SPC on VEC apoptosis and autophagy induced by deprivation of serum and @entity540 ( @entity540 ). SPC at 5-20     M inhibited apoptosis and induced autophagy in vitro. To understand the underlying mechanism, we investigated the role of integrin b4 in SPC-induced autophagy in VECs. SPC significantly decreased the level of integrin b4, whereas overexpression of integrin b4 inhibited SPC-induced autophagy. Moreover, knockdown of integrin b4 promoted VEC autophagy. To understand the downstream factors of integrin b4 in this process, we observed the effects of SPC on @entity2434 -specific phospholipase C (PC-PLC) activity and level of @entity876 . PC-PLC activity and @entity876 level in cytoplasm was decreased during autophagy induced by SPC, and knockdown of integrin b4 inhibited the activity of PC-PLC and the cytoplasmic level of @entity876 . SPC may promote autophagy via integrin b4. Moreover, PC-PLC and @entity876 may be the downstream factors of integrin b4 in autophagy of VECs deprived of serum and @entity540 .
