Detection of IgG-bound lipoprotein(a) immune complexes in XXXX with @entity301 .

@entity1 @entity844 @entity301 @entity75 @entity3452 @entity755 @entity1334 @entity11429 @entity731 @entity10058
BACKGROUND: LDL-immune complexes (IC) have a powerful pathogenic role for inducing foam cell formation in vitro more efficiently than any other known mechanism. Studies have also shown that plasma LDL-IC concentration is a powerful marker for the development of @entity731 . The structure, @entity844 composition and antioxidant concentrations of @entity3452 and LDL are quite similar. The same oxidation pattern has also been described for both lipoproteins. Modified forms of @entity3452 , some resembling oxidized @entity3452 , have been identified in @entity1 @entity75 . The existence of autoantibodies against MDA- @entity3452 in vivo is also presented. Therefore, we suppose that @entity3452 might trigger an immune response leading to the production of autoantibodies and subsequently to the formation of immune complexes. This study examined the existence of IgG-bound @entity3452 -IC and investigated its value as a risk factor for the development of @entity731 . METHODS: We developed two "sandwich" ELISAs for measuring plasma @entity3452 -IC and LDL-IC concentrations, using anti-human IgG( @entity1334 ) as the capture antibody, and quantitating with monoclonal anti-apo(a) or anti- @entity755 enzyme conjugate. Their concentrations were studied in 160 @entity1 with @entity301 ( @entity301 ) and 290 control subjects. RESULTS: Plasma TC, @entity11429 , TG and @entity755 concentrations in @entity301 @entity1 were all significantly increased, whereas HDL-C and apoAI concentrations were decreased. The @entity3452 concentrations in the @entity1 with @entity301 were also significantly different from those of control (262.4+/-220.0 vs. 211.3+/-199.4 mg/l, P<0.005). Plasma @entity3452 -IC (2.24+/-1.71 vs. 1.62+/-1.50 @entity10058 , P<0.0001) and LDL-IC (2.77+/-1.29 vs. 1.40+/-0.92 @entity10058 , P<0.0001) concentrations in @entity1 with @entity301 were both significantly higher than those of control. The relationships between @entity3452 -IC, LDL-IC concentrations and other lipid traits in all the studied subjects (n=450) were carried out. LDL-IC concentrations were positively correlated with @entity11429 , @entity755 , TC, TG and @entity3452 concentrations, while negatively correlated with HDL-C and apoAI concentrations, respectively. Similarly, @entity3452 -IC concentrations were positively correlated with @entity3452 , @entity11429 , @entity755 and TC concentrations, while negatively correlated with HDL-C and apoAI concentrations, respectively. Furthermore, a significantly positive relation between LDL-IC and @entity3452 -IC concentrations was also found (r=0.313, P<0.0001). CONCLUSIONS: We report the existence of @entity3452 -IC in both the plasma of @entity1 with @entity301 and control subjects. @entity3452 -IC concentration increases in the @entity301 @entity1 .
