A chloroplast @entity71873 is required for wound-induced XXXX accumulation in @entity958 .

@entity2271 @entity7214 @entity71873 @entity958 @entity33951
Plant lipoxygenases are thought to be involved in the biosynthesis of lipid-derived signaling molecules. The potential involvement of a specific @entity958 @entity71873 isozyme, @entity33951 , in the biosynthesis of the plant growth regulators @entity7214 (JA) and @entity2271 was investigated. Our characterization of @entity33951 indicates that the protein is targeted to chloroplasts. The physiological role of this chloroplast @entity71873 was analyzed in transgenic plants where cosuppression reduced @entity33951 accumulation. The reduction in @entity33951 levels caused no obvious changes in plant growth or in the accumulation of @entity2271 . However, the wound-induced accumulation of JA observed in control plants was absent in leaves of transgenic plants that lacked @entity33951 . Thus, @entity33951 is required for the wound-induced synthesis of the plant growth regulator JA in leaves. We also examined the expression of a wound- and JA-inducible @entity958 gene, vsp, in transgenic and control plants. Leaves of transgenic plants lacking @entity33951 accumulated less vsp mRNA than did control leaves in response to wounding. This result suggests that wound-induced JA (or some other @entity33951 -requiring component of the wound response pathway) is involved in the wound-induced regulation of this gene.
