An efficient strategy for establishing a model of sensorineural deafness in XXXX .

@entity1 @entity7913 @entity2167 @entity1483 @entity4494 @entity2319 @entity581 @entity35 @entity393
@entity2167 drugs can be used to produce a loss of cochlear hair cells to create animal models of @entity2319 . However, to the best of our knowledge, there is no report on the establishment of a @entity35 @entity2319 model through the combined application of @entity581 and loop diuretics. The aim of this study was to use single or combined administration of @entity1483 and @entity7913 to establish @entity35 models of @entity2319 . The @entity35 received intravenous injections of different doses of @entity1483 and/or intramuscular injections of @entity7913 . The auditory brainstem response was measured to determine the hearing threshold after drug application. Immunocytochemistry and confocal microscopy were performed to evaluate inner ear morphology. In the group receiving combined administration of @entity1483 and @entity7913 , the auditory brainstem response threshold showed significant elevation 3 days after administration, higher than that produced by @entity1483 or @entity7913 alone. The hair cells showed varying degrees of injury, from the apical turn to the basal turn of the cochlea and from the outer hair cells to the inner hair cells. The @entity4494 cells maintained a normal morphology during the first week after the hair cells completely disappeared, and then gradually degenerated. After 2 months, the majority of @entity4494 cells disappeared, but a few remained. These findings demonstrate that the combined administration of @entity1483 and @entity7913 has a synergistic @entity2167 effect, and that these drugs can produce hair cell loss and @entity393 in @entity35 . These findings suggest that even in @entity1 with severe @entity2319 , electronic cochlear implants may partially restore hearing.
