Antigens associated with @entity1 XXXX defined by murine monoclonal antibodies.

@entity1 @entity3172 @entity70870 @entity5 @entity957 @entity2 @entity58438 @entity420 @entity9560 @entity70868 @entity70869 @entity70867 @entity9861 @entity694 @entity2220
A panel of 12 monoclonal antibodies that preferentially react with @entity1 @entity2 cells has been produced. All are reactive with fresh frozen sections of @entity957 tissues in immunoperoxidase assays and are unreactive with lymphoblastoid cells, red blood cells, and fibroblasts in enzyme-linked immunosorbent assay. At least eight of these antibodies interact with cell surface components. These reagents can be subdivided into four groups based upon their reactivities. Groups 1 to 3 are unreactive with normal liver, lung, kidney, @entity694 in immunoperoxidase assays. Group 1 antibodies ( @entity58438 , @entity70867 , @entity70868 , @entity70868 , and @entity58438 ) are all of @entity3172 subclass and immunoprecipitate nonsulfated glycoprotein components with molecular weights of 80,000 and 180,000 and a nonglycosylated polypeptide with a molecular weight of 38,000. Group 1 antibodies are also reactive with some @entity2220 and, with the exception of @entity58438 , stain certain differentiated strata within normal adult plantar and fetal epidermis. Group 2 antibodies (PF2/A and @entity9560 ) react also with breast, gastric, and @entity694 and some @entity5 of neuroectodermal origin. Group 2 antibodies, which are both of @entity3172 subclass immunoprecipitate a nonglycosylated Mr 24,000 polypeptide. Group 3 antibodies (PF3/A, an IgG1; @entity9560 , an IgGM; and @entity9861 , an IgG2a) react additionally with certain other @entity5 , as well as with normal adult and fetal epidermis. Group 4 antibodies ( @entity70869 , an IgG2a; and @entity70870 , an IgG1) are less specific than those of the preceding groups, as they react with some normal tissues, including pancreatic islets and pneumocytes, as well as with a variety of @entity420 and @entity5 of neuroectodermal origin. @entity70869 and @entity70870 immunoprecipitate Mr 100,000 and 95,000 glycoproteins, respectively.
