( @entity15533 )-PCR fingerprinting of lactobacilli isolated from cervix of healthy XXXX .

@entity1 @entity15533 @entity10688 @entity24046 @entity880 @entity9206 @entity10265
A group of lactobacilli isolated from the cervix of 31 healthy @entity1 was characterized by ( @entity15533 )-polymerase chain reaction (PCR) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. Obtained fingerprints were compared with profiles available in an in-house database of the CCM bacteria collection covering type and reference strains of multiple @entity880 bacteria including lactobacilli. Selected strains representing individual clusters were further identified by pheS gene sequencing. In total, six @entity10265 species were found among lactobacilli isolated from the cervix of healthy @entity1 . The ( @entity15533 )-PCR method identified @entity24046 (11 strains), @entity10688 (one), and some of the @entity10265 jensenii strains (eight out of 11), but failed to identify the remaining strains, including the @entity10265 (18), @entity10265 mucosae (one), and @entity10265 vaginalis (one) species. L. jensenii strains were distributed over two fingerprint clusters. The majority of samples was dominated by one ( @entity15533 )-PCR type. The rep-PCR fingerprinting using the ( @entity15533 ) primer allowed straightforward identification of many, but not all, isolates. This method has been shown to be a useful tool for fast screening and grouping of vaginal lactobacilli, but its combination with another identification method is needed to obtain reliable identification results. In addition, @entity9206 was not shown to be the most common inhabitant of the female genital tract as generally assumed.
