Effects of @entity1246 on action of gastrin-releasing peptidergic neurons in exocrine secretion of isolated, perfused XXXX pancreas.

@entity35 @entity1027 @entity1246 @entity17633
INTRODUCTION: @entity1246 ( @entity1246 ) has been reported to enhance exocrine secretion evoked by intrinsic neuronal excitation in the pancreas. AIM: To see the effect of @entity1246 on the action of @entity17633 ( @entity17633 )ergic neurons in exocrine secretion of the pancreas. METHODOLOGY: Pancreatic neurons were excited by electrical field stimulation (EFS) in the isolated, perfused @entity35 pancreas. @entity17633 in the pancreatic circulation was neutralized by an anti- @entity17633 antiserum to block GRPergic neuronal action on pancreatic exocrine secretion. RESULTS: @entity1246 (3, 10, 30 microM), given intra-arterially, elevated the EFS-evoked pancreatic secretions of fluid and amylase dose-dependently. An anti- @entity17633 antiserum (10 microL/mL: titer of 1:66,000) reduced the @entity1246 (10 microM)-enhanced EFS-evoked pancreatic secretions. Synthetic porcine @entity17633 -27 (30, 100, 300 p ) increased the pancreatic secretions dose-dependently, and these were further elevated by @entity1246 (10 microM). The anti- @entity17633 antiserum also reduced the @entity1246 -enhanced @entity17633 (100 p )-induced pancreatic secretions. @entity1027 (10 microM) reduced the enhancing effect of @entity1246 on pancreatic secretions evoked by EFS as well as @entity17633 . CONCLUSION: @entity1246 enhances pancreatic secretions evoked by EFS as well as @entity17633 , which is reduced by the anti- @entity17633 antiserum. The enhancing effects of @entity1246 on the EFS- and @entity17633 -induced pancreatic secretions are diminished by @entity1027 . The results indicate that @entity1246 enhances intrinsic GRPergic neuronal action on exocrine secretion via the @entity1246 (A) receptors in the @entity35 pancreas.
