Antigen-driven T cell clones can proliferate in vivo, eradicate disseminated XXXX , and provide specific immunologic memory.

@entity2047 @entity5 @entity19 @entity16671 @entity786 @entity3783 @entity1778 @entity3512
The aim of the current study was to determine the ability of antigen-driven cloned helper cell independent cytotoxic T lymphocytes (HITc) to proliferate and to survive in vivo and to mediate @entity5 therapy. The HITc clone utilized (denoted 1.B6) was specifically cytolytic to @entity16671 , a syngeneic Friend virus-induced @entity19 @entity1778 . Activation in vitro (48 hr) with @entity16671 induced secretion of @entity2047 ( @entity2047 ), expression of @entity3783 ( @entity3783 ), and in vitro proliferation. These cells could be "rested" for several weeks without stimulation, which resulted in reduced expression of @entity3783 ; however, restimulation with antigen resulted in reinduction of @entity3783 and proliferation. The ability of cloned HITc to proliferate and to survive in vivo was examined in @entity786 (CY) pretreated donor @entity19 congenic for the @entity3512 gene. Adoptively transferred cloned HITc could be found in large numbers, and were widely distributed in vivo 1 wk after transfer. In @entity5 therapy, 1.B6 cells when injected into a site of @entity5 (i.p.) and used as an adjunct to CY were effective against disseminated @entity16671 . In this circumstance, cloned 1.B6 cells could be recovered from cured @entity19 125 days after transfer and were shown to specifically @entity5 and proliferate in vitro in response to @entity16671 . Thus as an adjunct to CY, @entity5 -specific cloned HITc are capable of eradicating disseminated @entity1778 , persisting long-term in vivo, and providing specific immunologic memory.
