Indirect immunohistochemistry and immunoelectron microscopy distribution of eight epidermal-dermal junction epitopes in the @entity1707 and in isolated perfused skin treated with XXXX .

@entity1 @entity1707 @entity27889 @entity4530 @entity30783 @entity2033 @entity599 @entity1068 @entity12961
@entity599 mustard ( @entity30783 , @entity2033 ) is a potent cutaneous vesicant that causes gross blisters by separation of the epidermal-dermal junction (EDJ). The EDJ of the skin is a highly specialized and complex structure composed of several components and plays a major role in the integrity of the skin. The isolated perfused porcine skin flap (IPPSF) was dosed with 0.2 mg/ml (n = 4), 5.0 mg/ml (n = 4), and 10.0 mg/ml (n = 5) @entity2033 or @entity1068 (n = 4) for 8 hr (dose-response study) and 10.0 mg/ml @entity2033 or @entity1068 for 1, 3, 5, and 8 hr (n = 4/treatment) (time-response study). Successful EDJ mapping was carried out in normal @entity1707 skin (NPS), @entity1068 -treated IPPSFs, and @entity2033 -treated IPPSFs using the following antibodies: laminin, type IV collagen, fibronectin, GB3 ( @entity12961 ), bullous pemphigoid (BP), and @entity27889 ( @entity27889 ). Two mouse anti-human monoclonal antibodies, L3d and 19-DEJ-1 (Uncein), did not cross-react with the EDJ of the pig. Antibody staining in NPS, ranging from very intense for laminin and type IV collagen to weak for fibronectin, was generally more discrete than in the IPPSF. No differences in staining were noted between the @entity1068 and nonblistered areas of the @entity2033 -treated IPPSFs. In @entity2033 -blistered areas, BP stained only the epidermal hemidesmosomes, and laminin, fibronectin, and GB3 stained primarily the dermis with fragments attached to the basal pole of the stratum base cells, while type IV collagen and @entity27889 stained only the dermis. Mapping of these epitopes determined that the precise plane of EDJ separation in the @entity2033 -treated skin occurred beneath the hemidesmosomes within the upper portion of the lamina lucida. The conservation of @entity1 epitopes in the EDJ of the @entity1707 further emphasizes the similarities between @entity1 skin and @entity1707 skin. Therefore, @entity1707 skin and the IPPSF may be used to study @entity2033 -induced vesication and @entity4530 .
