@entity1760 uptake of @entity958 transporters, @entity71350 and @entity71351 , expressed in @entity526 mutants: Complementation and growth inhibition by XXXX .

@entity71351 @entity3775 @entity1025 @entity958 @entity526 @entity15484 @entity71350 @entity1760
UNASSIGNED: @entity1760 ( @entity1025 ) plays a critical role in many physiological processes. @entity1025 transport systems in Salmonella have been well documented, but those in @entity526 have not been fully elucidated. We examined the effects of corA, mgtA, yhiD and corC gene deletion on @entity1025 transport in @entity526 . We obtained every combination of double, triple and quadruple mutants. The corA and mgtA double mutant required addition of 10mMMg(2+) to Luria-Bertani (LB) medium for growth, and the corA, mgtA and yhiD triple mutant TM2 required a higher @entity1025 concentration. The @entity1025 requirement of the quadruple mutant was similar to that of TM2. The results demonstrated that either CorA or MgtA is necessary for normal @entity526 growth in LB medium and that YhiD plays a role in @entity1025 transport under high @entity1025 growth conditions in @entity526 . The @entity958 @entity1025 transporters, @entity71350 and @entity71351 , were heterologously expressed in TM2 cells. TM2 cells expressing @entity71350 and @entity71351 could grow in LB medium that had been supplemented with 1mMMg(2+) and without @entity1025 supplementation, respectively, and cell growth was inhibited by 2mM @entity15484 . The results indicated that the growth of TM2 expressing @entity71350 and @entity71351 reflected these AtMRS2 function for @entity1025 and @entity3775 . The @entity526 TM2 cells are useful for functional analysis of @entity958 MRS2 proteins.
