[Effect of combined use of PDTC and XXXX on proliferation and invasion of @entity1 @entity0 cell line MCF-7].

@entity1 @entity9135 @entity2451 @entity0 @entity1311 @entity29276 @entity2843 @entity98 @entity1495 @entity14568
This investigation was made with special reference to the effect of the combined use of @entity2451 ( @entity2451 ) inhibitor @entity29276 dithiocarbamate( @entity14568 ) and @entity1311 on the expression of Matrix metalloproteinases ( @entity1495 ) and its inhibitor @entity2843 , and on the proliferation and invasion of @entity1 @entity0 cell line MCF-7. The MCF-7 cells were treated with PDTC and @entity1311 . The effect on proliferation was evaluated by @entity98 assay. The cell cycle was analyzed by flow cytometry. Western blot was used to determine the change of @entity9135 , @entity1495 and @entity2843 expression in MCF-7 cells after treatment. RT-PCR was used to detect @entity2451 @entity9135 mRNA expression. The invasion ability of MCF-7 cells was tested by the invasion, migration and cell adhesion assay. The cell growth was significantly slowed down and the cell cycle was arrested at G0/G1 phase after the combined treatment. The expression of @entity9135 and @entity1495 was down-regulated and the invasion ability of MCF-7 cells was decreased after the combined treatment. In conclusion,PDTC combined with @entity1311 effectively inhibited cell proliferation, induced cell cycle arrest, and decreased cell invasion ability of @entity0 MCF-7 cells. The mechanism may be associated with the inhibiting effect of PDTC on the @entity2451 -related gene expression.
