Study on XXXX resistance and prevalence of blaVIM1 and blaVIM2 metallo-beta lactamases among clinical isolates of @entity796 from Mashhad, Northeast of Iran.

@entity1 @entity2892 @entity796 @entity281 @entity2893 @entity580 @entity15598
BACKGROUND AND OBJECTIVES: The main cause of serious @entity580 is a Gram-negative pathogen known as @entity796 ( @entity796 ). Carbapenems are widely used as an appropriate treatment for these @entity281 , however resistance to these agents has been observed and is increasing. Metallo beta-lactamase (MBLs) enzyme is one of the main causes of resistance to @entity2893 . In the current study the frequency and production of VIM1 and @entity15598 by @entity2892 -resistant @entity796 isolates of @entity1 hospitalized in Imam Reza hospital were evaluated. MATERIALS AND METHODS: In this study, 131 clinical samples were collected from @entity1 hospitalized in Imam Reza hospital in Mashhad during a 15-month period from May 2011 to November 2012. After verification of P. aeruginosa isolates, antibiotic resistance patterns of isolates were determined for 14 antibiotics by Kirby-Bauer standard disk diffusion according to the CLSI guidelines. Combined-disk test was used for phenotypic determination of MBLs-producing isolates and after DNA extraction, genotypic determination of VIM1 and @entity15598 metallo beta-lactamase genes was carried out using Multiplex-PCR. RESULTS: Of 63 @entity2892 -resistant isolates (48.5%), 56 (88.8%) were MBL-producing in phenotypic assessments. Also amongst @entity2892 -resistant isolates, the frequency of VIM1 and @entity15598 genes were 58.7 and 3.17%, respectively. CONCLUSION: The results of the current study along with the results of the other conducted studies in Iran in recent years demonstrate that the average resistance to @entity2892 in @entity796 isolates was 51.3% which has increased in comparison with the results in 2006 (32.9%). It was also determined that the frequency of VIM1 gene was more than @entity15598 gene. In phenotypic assessment by using CD method, 49.6% of isolates were determined as MBLs-producing. The sensitivity and specificity of this method were verified in comparison with the results of PCR test.
