Quantification of G250 mRNA expression in XXXX by real-time reverse transcription-PCR of dissected tissue from paraffin sections.

@entity1 @entity1637 @entity5 @entity1358 @entity11567 @entity703
AIM: @entity1358 ( @entity1358 ) represents the most common @entity703 and is resistant to conventional therapies. G250 is a @entity5 -associated antigen and is expressed mainly in clear cell @entity1358 . It is a potential therapeutic target as well as a diagnostic marker for @entity1358 . Previous studies relating to G250 protein or G250 mRNA expression in @entity1358 using @entity1 tissue have largely been limited to immunohistochemical or conventional RT-PCR approaches that lack reproducibility, are subject to observer bias and are at most semi-quantitative. In this study, we evaluated the feasibility of using real-time RT-PCR in quantifying G250 mRNA expression using tissue dissected from paraffin sections of @entity703 . Using this approach, we performed a preliminary study investigating the relationship between G250 expression and @entity5 behavior in clear cell @entity1358 . METHODS: A total of 53 radical nephrectomy specimens with @entity703 (nine @entity1637 , seven chromophobe @entity1358 , four papillary @entity1358 and 33 clear cell @entity1358 ) were included in this study. SYBR Green real-time RT-PCR was performed using the ABI PRISM 7700 Sequence Detection System. A @entity11567 ( @entity11567 ) was used as an endogenous reference gene. RESULTS: Our study showed that real-time RT-PCR is a reliable approach in quantifying G250 mRNA expression using paraffin sections. Using this methodology, G250 mRNA was detected in all of the clear cell RCCs, but none of the other types of @entity5 studied or in normal kidney tissue. Our study also suggested that the expression of G250 mRNA might correlate with @entity5 stage and overall survival. CONCLUSION: Among common @entity703 , G250 is a specific marker for clear cell @entity1358 . Quantification of G250 mRNA expression by real-time RT-PCR using paraffin sections is a feasible approach for future clinical studies in evaluating the prognostic and predictive value of G250 in clear cell @entity1358 .
