Kefir induces cell-cycle arrest and apoptosis in XXXX -negative malignant T-lymphocytes.

@entity1 @entity981 @entity137 @entity1476 @entity5 @entity310 @entity7790 @entity2533 @entity1495 @entity2662
BACKGROUND: @entity981 ( @entity981 ) is a malignancy that occurs in white blood cells. The overall cure rate in @entity1 is 85%, whereas it is only 40% in adults. Kefir is an important probiotic that contains many bioactive ingredients, which give it unique health benefits. It has been shown to control several cellular types of @entity5 . PURPOSE: The present study investigates the effect of a cell-free fraction of kefir on CEM and Jurkat cells, which are @entity1 T-lymphotropic virus type I (HTLV- @entity2662 )-negative malignant T-lymphocytes. METHODS: Cells were incubated with different kefir concentrations. The @entity137 of the compound was evaluated by determining the percentage viability of cells. The effect of all the noncytotoxic concentrations of kefir on the proliferation of CEM and Jurkat cells was then assessed. The levels of @entity310 ( @entity7790 ), @entity2533 ( @entity2533 ), @entity1476 ( @entity1476 ), and @entity1495 mRNA upon kefir treatment were then analyzed using reverse transcriptase polymerase chain reaction (RT-PCR). Finally, the growth inhibitory effects of kefir on cell-cycle progression/apoptosis were assessed by Cell Death Detection (ELISA) and flow cytometry. RESULTS: The maximum @entity137 recorded after 48-hours treatment with 80  g/ L kefir was only 42% and 39% in CEM and Jurkat cells, respectively. The percent reduction in proliferation was very significant, and was dose-, and time-dependent. In both cell lines, kefir exhibited its antiproliferative effect by downregulating @entity7790 and upregulating @entity2533 mRNA expression. Upon kefir treatment, a marked increase in cell-cycle distribution was noted in the preG(1) phase of CEM and Jurkat cells, indicating the proapoptotic effect of kefir, which was further confirmed by Cell Death Detection ELISA. However, kefir did not affect the mRNA expression of metalloproteinases needed for the invasion of leukemic cell lines. CONCLUSION: In conclusion, kefir is effective in inhibiting proliferation and inducing apoptosis of @entity2662 -negative malignant T-lymphocytes. Therefore, further in vivo investigation is highly recommended.
