A simple method for XXXX quantification in plasma using solid-phase extraction and reversed-phase high-performance liquid chromatography.

@entity1040 @entity1042 @entity1626 @entity95 @entity3931 @entity3360 @entity10457
A simple and sensitive reversed-phase liquid chromatography method was developed and validated for the determination of @entity3360 (NC) in @entity95 plasma. @entity3360 and @entity3931 , used as internal standard, were initially extracted from plasma by a rapid solid-phase extraction using C(18) cartridges. After extraction, @entity3360 was separated by HPLC on a C(18) column and quantified by ultraviolet detection at 254 nm. A mixture of @entity1626 -0.02 M @entity10457 buffer- @entity1042 (45:40:15) with 0.2% of @entity1040 of pH of 6.1 was used as mobile phase. The mean (+/-SD) extraction efficiency of NC was 77.56 +/- 5.4, 84.23 +/- 4.32 and 83.94 +/- 3.87% for drug concentrations of 5, 25 and 100 ng/mL, respectively. The method proved to be linear in the range of 5-100 ng/mL with a regression coefficient of 0.9993. The relative standard deviations of intra- and inter-day analysis for NC in plasma were 3.26-6.52% (n = 5) and 4.71-9.38% (n = 5), respectively. The differences of the mean value measured from the concentration prepared, expressed in percentages (bias percentage), were only - 5.2, 0.4 and 0.8% at NC 5, 25 and 50 ng/mL, which confirmed the accuracy of the method. The analytical technique was used to determine NC plasma concentration after drug oral administration to @entity95 . The results inferred that NC is rapidly absorbed in @entity95 and has a short half-life (t(1/2) = 1.34 h).
