Implementation of an in vitro model system for investigation of XXXX after renal ischemia.

@entity32 @entity4647 @entity63 @entity39 @entity1506 @entity1421 @entity16 @entity520 @entity519 @entity810 @entity35
Ischemic @entity810 (iAKI) is a common event in organ transplantation and may occur during severe surgery. To gain mechanistic insights into @entity63 -induced alterations at the level of proximal tubule cells we set up an in vitro model of @entity63 and reperfusion using the @entity35 proximal tubule cell line NRK-52E. In this particular model we simultaneously applied @entity520 , @entity519 and aglycemia together for 2h, using low volume buffer systems and a @entity519 chamber. Thereafter reperfusion was mimicked by subsequently culturing the cells for up to 48h under standard conditions. In order to validate the system we investigated whether effects that take place in existing in vivo models of @entity63 and reperfusion can be observed. Namely, induction of @entity39 , apoptosis and of @entity63 reperfusion induced protein (IRIP), dedifferentiation (alphaSMA), @entity32 ( @entity4647 ), @entity1506 ( @entity1506 ), release of @entity1421 and basolateral uptake of organic anions. In fact, all parameters developed as described for the in vivo situation during reperfusion after @entity63 . Taken altogether we have established an in vitro model of proximal tubule cell @entity16 after @entity63 , showing typical changes described in vivo. Additionally, our model system is suitable for isolated application of the typical insults associated with @entity63 (e.g. @entity520 alone, @entity519 alone, aglycemia alone), in order to obtain more insight into the mechanistic events that lead to @entity16 in the kidney on the cellular level.
