[Effect of triptolide on the expression of @entity16620 in the renal tissue of diabetic nephropathy XXXX ].

@entity35 @entity8 @entity16620 @entity6
OBJECTIVE: To investigate the effect of triptolide (TPL) on the renal tissue of @entity6 @entity35 and its possible mechanisms. METHODS: SD @entity35 were randomly divided into the normal control group (as the normal group), the @entity6 model group (the model group), the low dose TPL treatment group (the low dose TPL group, TPL 0.2 mg/kg by gastrogavage), the high dose TPL treatment group (the high dose TPL group, TPL 0.4 mg/kg by gastrogavage). Equal volume of normal saline was given to @entity35 in the normal group and the model group. Five @entity35 were randomly selected from each group at week 4, 8, and 12 of the experiment to detect body weight, kidney weight, 24 h urinary albumin (24 h UAL), plasma glucose (FBG), total cholesterol (TC), total triglyeride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), white blood cell (WBC), and hemoglobin A1c (HbA1c). The mRNA and protein expression of @entity16620 ( @entity16620 ) in the renal tissue was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA). The renal tissue was pathologically stained by HE, PAS, and Masson staining. The glomerular and renal tubular interstitial lesions were observed at each time point. The @entity8 index (GSI) was observed by PAS staining, and the @entity8 ( @entity8 ) was calcutated. RESULTS: Compared with the same group at week 4, the expression of 24 h UAL, @entity16620 , GSI, and @entity8 at week 12 significantly decreased in two TPL groups (P <0.01). Compared with the same group at week 8, the expression of 24 h UAL, @entity16620 , GSI, and @entity8 at week 12 also significantly decreased in the two TPL groups (P <0. 05, P <0.01). Compared with the normal group, body weight and the kidney weight obviously decreased at week 4, 8, and 12 in the model group (P <0. 01); 24 h UAL, FBG, TG, TC, HbA1c, @entity16620 , GSI, and @entity8 were obviously elevated (P <0.01). Compared with the model group, 24 h UAL, @entity16620 , GSI, and @entity8 also decreased in the two TPL treatment groups (P <0.01). Compared with the low dose TPL group, they were attenuated in the high dose TPL group (P <0. 05, P <0. 01). CONCLUSION: TPL could not only inhibit the over-expression of @entity16620 , but also improve the @entity8 and @entity8 in the renal tissue of @entity6 @entity35 .
