Monocyte factors modulate in vitro T-lymphocyte mitogenesis in XXXX .

@entity1949 @entity308 @entity1421 @entity95 @entity665 @entity3147
This study investigated changes in T-lymphocyte mitogenesis and immunoregulatory cytokines during @entity3147 . In vitro T cell response to concanavalin A was compared among @entity1949 ( @entity1949 ), energy restricted pair fed control (PF), and ad libitum control (C) @entity95 . Cell cultures were supplemented with crude monocyte supernatants (CMS) from @entity1949 , PF or C animals at either 1% or 8% final concentration in culture. @entity1421 ( @entity1421 ) concentration of unstimulated or stimulated lymphocyte culture supernatants and CMS was determined. Lymphocyte cultures from @entity1949 , PF and C animals had enhanced @entity665 incorporation when supplemented with C and/or PF derived CMS. Addition of 8% CMS from @entity1949 @entity95 inhibited proliferation below levels observed in mitogen-only stimulated groups in all cultures. At the 1% concentration, inhibition was seen in @entity1949 and C derived cells cultures and modest enhancement was seen in PF cultures. @entity1421 concentration in supernatants from stimulated and unstimulated lymphocyte cultures from @entity1949 @entity95 were higher than in C and PF cell cultures. These results suggest (a) that under appropriate culture conditions lymphocytes from @entity1949 donors are capable of enhanced proliferation and (b) that @entity308 cell mitogenesis observed in @entity3147 may reflect alterations in immunoregulatory signals. The role of interleukin 1 (IL-1) and @entity1421 in the modulation of this response is discussed.
