T cell antigen receptors in XXXX .

@entity1 @entity1569 @entity2743 @entity1252 @entity405 @entity1723 @entity3039 @entity792 @entity309
Three mAb to variable region determinants of the alpha/beta-chain TCR were used to detect discrete populations of peripheral blood T cells. T cells sharing a TCR determinant defined by such an antibody presumably use the same or similar TCR V or J genes for their alpha- or beta-chains. Thus analysis with these mAb provides a tool to investigate TCR gene usage and expression. Since autoantigen specific T cells may play an important role in initiating @entity792 , TCR were analyzed in different @entity792 and control groups including @entity309 , @entity2743 , @entity3039 , @entity1569 , @entity1252 , @entity1723 , and in nonautoimmune control diseases and normals. Purified T cells were stained by indirect immunofluorescence with three mAb to TCR variable regions: mAb S511 stains 1.8 +/- 0.9% (mean +/- 2 SD), mAb C37 stains 3.4 +/- 1.5% and mAb OT145 stains from 0 to 6% of T cells from normal donors. Several individuals were identified with expanded subsets of positive T cells. One @entity1 with adult ITP followed during a 12-mo period consistently had elevated percentages of T cells staining with the mAb OT145 (15.9 to 24.5%). These cells were found to be exclusively @entity405 +. By Southern blotting DNA prepared from these OT145+, @entity405 + cells, but not DNA from the @entity1 's OT145- T cells, revealed a clonal rearrangement using a beta-chain C region probe. Thus this @entity1 had a monoclonal expansion of @entity405 +, OT145+ cells. Hyperexpression of a TCR variable region, as defined by the available mAb, could not be associated with any of the diseases studied. Examination of T cells at the site of @entity792 , such as T cells from @entity309 synovial fluid, revealed normal percentages of cells staining with these mAb. Immunoperoxidase staining of psoriatic lesional skin showed no striking enrichment of T cells bearing one or the other TCR type.
