Side Population Cell Level in XXXX Breast Cancer and Factors Related to Disease-free Survival.

@entity1 @entity5956 @entity0 @entity5 @entity3
Side population (SP) cells have stem cell-like properties with a capacity for self-renewal and are resistant to chemotherapy and radiotherapy. Therefore the presence of SP cells in @entity1 @entity0 probably has prognostic value. OBJECTIVE: To investigate the characteristics of SP cells and identify the relationship between the SP cells levels and clinico-pathological parameters of the @entity0 and disease-free survival (DFS) in @entity0 @entity1 . MATERIALS AND METHODS: A total of 122 eligible @entity0 @entity1 were consecutively recruited from January 1, 2006 to December 31, 2007 at Yunnan Tumor Hospital. All eligible subjects received conventional treatment and were followed up for seven years. Predictors of recurrence and/or @entity3 and DFS were analyzed using Cox regression analysis. @entity1 @entity0 cells were also obtained from fresh @entity1 @entity0 tissue and cultured by the nucleic acid dye @entity5956 with Verapami. Flow cytometry (FCM) was employed to isolate the cells of SP and non-SP types. RESULTS: In this study, SP cells were identified using flow cytometric analysis with @entity5956 dye efflux. Adjusted for age, @entity5 size, lymph nodal status, histological grade, the Cox model showed a higher risk of recurrence and/or @entity3 positively associated with the SP cell level (1.75, 1.02-2.98), as well as with axillary lymph node @entity3 (2.99, 1.76-5.09), pathology invasiveness type (1.7, 1.14-2.55), and @entity5 volume doubling time (TVDT) (1.54, 1.01-2.36). CONCLUSIONS: The SP cell level is independently associated with @entity5 progression and clinical outcome after controlling for other pathological factors. The axillary lymph node status, TVDT and the status of non-invasive or invasive @entity5 independently predict the prognosis of @entity0 .
