Heterogeneity of XXXX gene expression in normal and neoplastic tissues.

@entity263 @entity0 @entity5091 @entity2 @entity73287 @entity11092 @entity3558 @entity5087 @entity2355 @entity694 @entity420 @entity356
To determine the relative expression of distinct @entity3558 genes in normal and neoplastic tissue, antibodies and cDNA probes that recognize the core tandem repeat sequences of membrane-bound ( @entity2355 ) and secreted ( @entity5091 and @entity5087 ) mucins were used for immunohistochemical and RNA Northern and slot-blot analysis. @entity2355 mRNA was detected in all epithelial tissues tested. @entity2355 core peptide, recognized by monoclonal antibodies 139H2 and DF3, was highly expressed on apical membranes of bronchus, breast, salivary gland, pancreas, prostate, and uterus, and was sparsely expressed in gastric surface cells, gallbladder, small intestine, and colonic epithelium. In contrast, @entity5091 and @entity5087 gene expression was primarily restricted to the intestinal tract. @entity5091 mRNA was highly expressed in normal jejunum, ileum, and colon, compared with very low levels in normal bronchus and gallbladder. @entity5087 mRNA was highly expressed in normal jejunum, ileum, colon, and gallbladder. Immunohistochemical studies using antibodies against synthetic @entity5091 (anti- @entity11092 ) and @entity5087 (anti-M3P) peptides indicate that @entity5091 - and @entity5087 -producing cells in the gastrointestinal tract are distinct. Goblet cells of the small intestine and colon reacted strongly with anti- @entity11092 , whereas M3P reactivity was restricted to columnar cells of small intestinal villi, surface colonic epithelium, and gallbladder. @entity3558 protein epitopes and mRNA levels were frequently altered in @entity420 compared to corresponding normal tissues. Alterations included increased expression, aberrant expression, and, less frequently, loss of expression. Increased @entity2355 immunoreactivity was observed in most @entity420 of the breast, lung, stomach, pancreas, prostate, and ovary. In addition, with the exception of @entity263 , focal aberrant expression of @entity5091 and @entity5087 epitopes was frequently observed. Increased @entity2355 , @entity5091 , and @entity5087 epitopes were present in @entity694 of all histological subtypes, with the greatest increase of @entity5091 epitopes observed in colloid (mucinous) @entity694 . @entity5091 or @entity5087 mRNA levels were increased in colloid @entity694 compared with normal colon, however in well- and moderately well-differentiated @entity694 @entity73287 mRNA levels were decreased. Compared with corresponding normal tissue, @entity2355 mRNA levels were increased in @entity0 and well-differentiated @entity2 , and @entity5087 mRNA was increased in @entity356 . Normal stomach lacked both @entity5091 and @entity5087 immunoreactivity and mRNA, however, @entity5091 and @entity5087 proteins and mRNA were highly expressed in @entity356 . In conclusion, @entity3558 genes are independently regulated and their expression is organ- and cell type-specific. Furthermore, neoplastic transformation is associated with dys-regulated expression of both membrane-bound and secreted @entity3558 core protein epitopes and may be due to altered @entity3558 mRNA levels and/or altered @entity3558 glycosylation.
