Effects of in utero and in vitro incubation on the lipid-bound @entity1718 and XXXX of porcine spermatozoa.

@entity844 @entity19980 @entity3150 @entity5068 @entity1718 @entity165
Sperm-rich semen and washed porcine spermatozoa were incubated for up to 2 hr either in utero in the presence of oviduct fluid or in vitro at 37 degrees C. Sperm lipids were extracted and separated into phospholipid and neutral lipid fractions. Eleven phospholipid and five neutral lipid @entity1718 were identified and quantified using GC and GC-MS. The percentage of 22:5n6, the major phospholipid @entity844 , decreased slightly but significantly during 1.5 hr of in utero incubation (41.2-38.0%), but after 2.0 hr of in utero incubation no significant difference was observed (40.0%). None of the phospholipid @entity1718 changed in concentration during in vitro incubation. The mole ratio of phospholipid to phospholipid @entity844 (1.00:1.27) did not change during incubation. The levels of neutral lipid-bound 14:0 decreased (43.5% to 31.8%) and that of 18:0 increased (11.1% to 18.2%) during in utero incubation. Similar but less pronounced changes were observed during in vitro incubation. (43.5% to 36.0%; and 11.1% to 15.8%, respectively). Two major @entity3150 , @entity165 (73%) and @entity19980 (27%) were identified by gas chromatography-mass spectrometry. The mole ratio of phospholipid to @entity5068 (2.47:1:00) did not change during incubation.
