A new insight into XXXX stem cell markers: Could local and circulating @entity5 stem cell markers correlate in @entity14 ?

@entity1 @entity5 @entity15739 @entity15738 @entity14
UNASSIGNED: Cancer stem cell (CSC) markers could serve as potential prognostic procedure. This study is aimed to investigate the local expression of @entity15738 ( @entity15738 ) and @entity15739 in @entity14 ( @entity14 ) at both protein and messenger RNA (mRNA) level, followed by providing a comparison of the local and circulating expression pattern of these markers, based on our present and previous study. The mRNA expression level of @entity15738 and @entity15739 was evaluated using comparative real-time PCR method applying 58 fresh @entity5 tissues and their correspondent normal margins. Immunohistochemistry was applied to analyze the protein expression level of @entity15738 and @entity15739 in paraffin-embedded @entity14 tissues. The correlation of @entity15738 and @entity15739 expression pattern with clinicopathological characteristics was assessed. A higher mRNA expression level of @entity15738 (3.28-fold change, p   <   0.001) and @entity15739 (2.29-fold change, p   <   0.001) was observed in @entity14 fresh tissues compared to the normal adjacent margins, and the expression level was higher in @entity1 with higher grade and stages of disease and @entity1 who underwent neoadjuvant chemoradiotherapy (CRT). The protein expression level of @entity15738 and @entity15739 was also increased significantly in @entity5 tissues compared to normal colon tissues which were positively correlated to @entity5 stage and grade and neoadjuvant CRT. Taken together, the results of protein analysis were in accordance with mRNA assessment. The local expression pattern of @entity15738 and @entity15739 was also in accordance with their expression level in circulation. However, some minor inconsistencies were observed which may be attributed to several factors including the possible effect of CRT on CSC reprogramming.
