Immunohistochemical study of Ca2+/calmodulin-dependent protein kinase II in the XXXX brain using a specific monoclonal antibody.

@entity535 @entity18277 @entity460 @entity238 @entity27114
To analyze the distribution of @entity238 @entity460 /calmodulin-dependent protein kinase II ( @entity27114 ) in the adult brain, we generated monoclonal antibodies against the bacterially expressed 490- @entity535 (a.a.) form of @entity27114 . One of those, named #18 antibody, was used for this study. Western blot analysis of the adult head extracts showed that the antibody specifically detects multiple bands between 55 and 60 kDa corresponding to the molecular weights of the splicing isoforms of @entity27114 . Epitope mapping revealed that it was in the region between 199 and 283 a.a. of @entity27114 . Preferential @entity27114 immunoreactivity in the embryonic nervous system, adult thoracic ganglion and gut, and larval neuro-muscular junction (NMJ) was consistent with previous observations by in situ hybridization and immunostaining with a polyclonal antibody at the NMJ, indicating that the antibody is applicable to immunohistochemistry. Although @entity27114 immunoreactive signal was low in the retina, it was found at regular intervals in the outer margin of the compound eye. These signals were most likely to be interommatidial bristle mechanosensory neurons. @entity27114 immunoreactivity in the brain was observed in almost all regions and relatively higher staining was found in the neuropilar region than in the cortex. Higher @entity27114 immunoreactivity in the @entity18277 ( @entity18277 ) was found in the entire gamma lobe including the heel, and dorsal tips of the alpha and alpha' lobes, while cores of alpha and beta lobes were stained light. Finding abundant @entity27114 accumulation in the gamma lobe suggested that this lobe might especially require high levels of @entity27114 expression to function properly among @entity18277 lobes.
