Regulation of natriuretic peptide receptor A and B expression by XXXX in cultured aortic smooth muscle cells.

@entity101 @entity8262 @entity28353 @entity12579 @entity31246 @entity1810 @entity35
Two types of natriuretic peptide receptors ( @entity28353 and @entity31246 ) are membrane guanylate cyclases whose relative expression varies in different tissues. Because natriuretic peptides have been shown to inhibit aortic smooth muscle proliferation, we investigated the regulation of @entity28353 and @entity31246 in these cells under different proliferative conditions. NPR subtype mRNA levels were measured by our newly developed quantitative reverse transcription-polymerase chain reaction assay using mutated @entity28353 and @entity31246 cRNA as internal standards. The functional impact of their expression was determined by @entity12579 (ANP)- and C-type natriuretic peptide (CNP)-induced stimulation of @entity8262 production. In the intact aorta, @entity31246 mRNA levels were found to be 10-fold higher than those of @entity28353 . This dominance was further amplified (1000-fold) in long-term cultures (10 to 15 passages) of aortic smooth muscle cells (ASMC). Higher @entity8262 production with CNP than with ANP was observed in cultured ASMC from @entity35 -Kyoto (WKY) @entity35 . Similar stimulation by the two agonists was noted in spontaneously @entity101 @entity35 (SHR) cells, paralleled by a 10-fold increase in @entity28353 mRNA levels and ANP stimulation of @entity8262 in @entity101 cells. The present study also evaluated @entity28353 and @entity31246 mRNA control by @entity1810 ( @entity1810 ), an important regulator of cell proliferation that is overexpressed in SHR ASMC. @entity1810 decreased both @entity28353 and @entity31246 mRNA levels with a predominant effect in SHR cells at high cell density.(ABSTRACT TRUNCATED AT 250 WORDS)
