Characterization of an inducible C2 H2 -type zinc finger transcription factor VuSTOP1 in XXXX bean (Vigna umbellata) reveals differential regulation between low pH and @entity3775 tolerance mechanisms.

@entity3775 @entity3736 @entity8108 @entity375 @entity579 @entity18839
UNASSIGNED: The @entity579 bean (Vigna umbellata) root apex specifically secretes @entity3736 through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under @entity3775 ( @entity18839 ) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from @entity579 bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the @entity375 one-hybrid assay. We characterized the function of VuSTOP1 in @entity18839   - and @entity8108 -tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by @entity18839 and @entity8108 stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in @entity8108 tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in @entity8108 tolerance, but only a minor role in @entity18839 tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
