Serum Proteome Signature of Radiation Response: Upregulation of Inflammation-Related Factors and Downregulation of Apolipoproteins and Coagulation Factors in @entity5 XXXX Treated With Radiation Therapy-A Pilot Study.

@entity1 @entity135 @entity137 @entity263 @entity1161 @entity5 @entity1003
PURPOSE: Ionizing radiation affects the proteome of irradiated cells and tissue, yet data concerning changes induced during @entity1161 ( @entity1161 ) in @entity1 blood are fragmentary and inconclusive. We aimed to identify features of serum proteome and associated processes involved in response to partial body irradiation during @entity5 treatment. METHODS AND MATERIALS: Twenty @entity1 with @entity135 ( @entity135 ) and 20 @entity1 with @entity263 received definitive intensity modulated @entity1161 . Blood samples were collected before @entity1161 , just after @entity1161 , and 1  month after the end of @entity1161 . Complete serum proteome was analyzed in individual samples, using a shotgun liquid chromatography-tandem mass spectrometry approach which allowed identification of approximately 450 proteins. Approximately 100 unique proteins were quantified in all samples after exclusion of immunoglobulins, and statistical significance of differences among consecutive samples was assessed. Processes associated with quantified proteins and their functional interactions were predicted using gene ontology tools. RESULTS: @entity1161 -induced changes were marked in the @entity135 @entity1 group: 22 upregulated and 33 downregulated proteins were detected in post- @entity1161 sera. Most of the changes reversed during follow-up, yet levels of some proteins remained affected 1  month after the end of @entity1161 . @entity1161 -upregulated proteins were associated with acute phase, inflammatory response, and complement activation. @entity1161 -downregulated proteins were associated with transport and metabolism of lipids (plasma apolipoproteins) and @entity1003 . @entity1161 -induced changes were much weaker in @entity263 @entity1 , which corresponded to differences in acute radiation @entity137 observed in both groups. Nevertheless, general patterns of @entity1161 -induced sera proteome changes were similar in both of the groups of @entity5 @entity1 . CONCLUSIONS: In this pilot study, we proposed to identify a molecular signature of radiation response, based on specific features of serum proteome. The signature included upregulation of factors involved in acute or inflammatory response but also downregulation of plasma apolipoproteins and factors involved in @entity1003 .
