Fluorescent magnetic XXXX nanoparticles for cardiac precursor cell selection from stromal vascular fraction and optimization for magnetic resonance imaging.

@entity137 @entity28224 @entity68 @entity1933 @entity319 @entity970 @entity968
Fluorescent magnetic @entity1933 nanoparticles have been used to label cells for imaging as well as for therapeutic purposes. The purpose of this study was to modify the approach to develop a nanoprobe for cell selection and imaging with a direct therapeutic translational focus. The approach involves physical coincubation and adsorption of superparamagnetic @entity1933 nanoparticle- @entity968 (SPION- @entity970 ) complexes with a monoclonal antibody (mAb) or a set of antibodies. Flow cytometry, confocal laser scanning microscopy, transmission electron microscopy, @entity68 staining, and magnetic resonance imaging were used to assess cell viability, function, and labeling efficiency. This process has been validated by selecting adipose tissue-derived cardiac progenitor cells from the stromal vascular fraction using @entity28224 ( @entity28224 )/kinase domain receptor ( @entity319 ) mAbs. These markers were chosen because of their sustained expression during cardiomyocyte differentiation. Sorting of cells positive for @entity28224 and @entity319 allowed the enrichment of cardiac progenitors with 90% troponin-I positivity in differentiation cultures. SPION labeled cardiac progenitor cells (1*10(5) cells) was mixed with gel and used for 3T magnetic resonance imaging at a concentration, as low as 12.5  g of @entity68 . The @entity137 assays, at cellular and molecular levels, did not show any detrimental effects of SPION. Our study has the potential to achieve moderate to high specific cell selection for the dual purpose of imaging and therapy.
