Albumin resuscitation increases cardiomyocyte contractility and decreases XXXX synthase II expression in @entity35 endotoxemia.

@entity296 @entity326 @entity568 @entity3207 @entity519 @entity842 @entity35 @entity551
OBJECTIVE: @entity551 and hypoperfusion during @entity326 may contribute to tissue @entity519 and the intramyocardial inflammatory response that results in @entity296 . Therefore, we hypothesized that crystalloid or colloid resuscitation may alter @entity296 . DESIGN: Randomized, controlled, prospective animal study. SETTING: University animal laboratory. SUBJECTS: @entity35 (250-300 g, n = 6/group). INTERVENTIONS: @entity35 received an intraperitoneal injection of 10 mg/kg lipopolysaccharide or control. One hour later, @entity35 were randomized to intravenous resuscitation and received either 30 mL/kg normal saline, 10 mL/kg 10% pentastarch, 10 mL/kg 5% @entity35 albumin, or no volume. MEASUREMENTS AND MAIN RESULTS: We measured fractional @entity3207 of cardiomyocytes isolated 5 hrs after lipopolysaccharide or control injection. In separate identical experiments, we measured myocardial @entity568 , macrophage inhibitory protein-2, and @entity842 synthase II protein and messenger RNA expression. Control fractional @entity3207 of 24.1 +/- 2.2% was decreased by lipopolysaccharide to 18.8 +/- 1.2% (p <.001). Volume resuscitation after lipopolysaccharide significantly improved fractional @entity3207 (p <.001). In particular, albumin resuscitation increased fractional @entity3207 to 23.5 +/- 0.9%, which was more than either saline (fractional @entity3207 20.1 +/- 1.7%,p <.01) or pentastarch (fractional @entity3207 21.4 +/- 0.9%,p <.01). Myocardial macrophage inhibitory protein-2 protein and @entity568 and macrophage inhibitory protein-2 messenger RNA expression and neutrophil content were elevated following lipopolysaccharide (p <.05) but were not altered by volume resuscitation. Myocardial @entity842 synthase II protein and messenger RNA expression increased following lipopolysaccharide (p <.01) and decreased with albumin resuscitation. CONCLUSIONS: We conclude that following lipopolysaccharide injection, volume resuscitation improves cardiomyocyte fractional @entity3207 . Albumin resuscitation is particularly beneficial in preventing reduced cardiomyocyte contractility, and this benefit may be related to an albumin-induced reduction in @entity842 synthase II protein and messenger RNA expression following endotoxin injection.
