Functional characterization of cytokine autoantibodies in XXXX @entity1 .

@entity1 @entity650 @entity177 @entity4105 @entity98 @entity1192
We have recently reported on an increase of @entity1192 and @entity650 autoantibodies in @entity1 maintained on chronic hemodialysis treatment. The aim of the present study was to evaluate functional properties of these autoantibodies. Serum samples of more then 500 @entity177 @entity1 with and without replacement therapy were screened for the presence of @entity1192 and @entity650 autoantibodies by second antibody precipitation. The neutralizing capacity of @entity1192 autoantibody serum was studied by immunofluorescence flow cytometry analysis of @entity1192 induced expression of @entity4105 ( @entity4105 , @entity4105 ) on HUVEC ( @entity1 umbilical vein endothelial cells). Results of these inhibition studies were confirmed with IgG preparations from antibody positive sera, purified by affinity chromatography. Functional studies on @entity650 -dependent B9 cell proliferation were performed with @entity650 autoantibody positive sera, and quantitated with the colorimetric @entity98 assay. @entity1192 induced expression of @entity4105 on HUVEC (considered 100% positive cells) was inhibited by each @entity1192 autoantibody positive serum sample (N = 13; anti- @entity1192 activity: 7.62 to 57.52% binding). Inhibition of @entity4105 expression by @entity1192 autoantibodies ranged from 0.11 to 80.22% positive cells (0.15 to 92.31% mean fluorescence intensity). A strong correlation of @entity4105 expression with @entity1192 autoantibody concentration was observed (P < 0.005). Furthermore, IgG eluates from autoantibody positive @entity1 inhibited @entity4105 expression to 41.0 +/- 23.1% positive cells if compared with 83.7 +/- 5.7% positive cells of the IgG depleted serum samples.(ABSTRACT TRUNCATED AT 250 WORDS)
