Gamma- @entity271 induced apoptosis is associated with unfolded protein response in human XXXX cells.

@entity39448 @entity270 @entity0 @entity5 @entity14544 @entity7952 @entity13791 @entity2533 @entity9190 @entity271 @entity23638 @entity165
@entity39448 (y-T3) is a member of the @entity270 family. @entity271 (T3s) are powerful antioxidants and possess anticancer, neuroprotective and @entity165 -lowering properties. @entity271 inhibit the growth of various @entity5 cell lines without affecting normal cells. Less is known about the exact mechanisms of action of T3s on cell death and other growth inhibitory pathways. In the present study, we demonstrate that y-T3 induces apoptosis in MDA-MB 231 and MCF-7 @entity0 cells as evident by @entity9190 cleavage and @entity14544 activation. Gene expression analysis of MCF-7 cells treated with y-T3 revealed alterations in the expression of multiple genes involved in cell growth and proliferation, cell death, cell cycle, cellular development, cellular movement and gene expression. Further analysis of differentially modulated genes using Ingenuity Pathway Analysis software suggested modulation of canonical signal transduction or metabolic pathways such as @entity13791 -mediated oxidative stress response, @entity2533 signaling and endoplasmic reticulum (ER) stress response. Analysis of ER-stress-related proteins in MCF-7 and MDA-MB 231 cells treated with y-T3 demonstrated activation of @entity7952 and pIRE1a pathway to induce ER stress. @entity23638 ( @entity23638 ) was identified as the most up-regulated gene (16.8-fold) in response to y-T3. @entity23638 knockdown using siRNA suggested an essential role of @entity23638 in y-T3-induced apoptosis. In summary, we demonstrate that y-T3 modulates ER stress signaling and have identified @entity23638 as a molecular target for y-T3 in @entity0 cells.
