Primary structure of a C-hordein gene from XXXX .

@entity1522 @entity2247 @entity1160 @entity368 @entity535 @entity377 @entity2733 @entity1853 @entity6778 @entity1521 @entity815 @entity1473
The @entity377 sequence of a 2065 base pair HindIII fragment, containing a gene (lambda hor1-14) belonging to the Hor1 locus in @entity2247 , has been determined. The fragment consists of 1044 bp of coding region interrupted by an amber codon at base 481, a 5' non-coding region of 428 bp and a 3' non-coding region with 593 bp. The deduced @entity535 sequence of the mature protein (327 @entity1522 ) is characterized by an octapeptide motif PQQPFPQQ which is repeated throughout the peptide chain between a unique 12 @entity535 long @entity2733 -terminal and an equally unique 10 @entity535 long @entity1473 -terminal end. The @entity1521 + @entity1160 content is 62% and the next three most abundant @entity1522 are @entity368 (9%), @entity815 (8%) and @entity6778 (3%). In the 5' non-coding region there is a TATA box at -98 bp from the start @entity1853 . The 3' non-coding region has a polyadenylation signal 76 bp downstream from the TAA stop codon. The deduced @entity535 sequences of the @entity2733 - and @entity1473 -terminals of lambda hor1-14 are very similar but not identical to those known from the Edman degradation and carboxypeptidase Y analysis of C-hordein polypeptides. The 3' coding and non-coding region of lambda hor1-14 is closely similar but different in detail to the known C-hordein cDNA clones. One polyadenylation signal is found in lambda hor1-14 whereas two are present in each of the three known C-hordein cDNAs. These differences and the amber codon interrupting the open reading frame indicate that this gene is silent.
