Balnearium lithotrophicum gen. nov., sp. nov., a novel thermophilic, strictly anaerobic, XXXX -oxidizing chemolithoautotroph isolated from a black smoker chimney in the Suiyo Seamount hydrothermal system.

@entity3884 @entity70597 @entity6734 @entity56261 @entity544 @entity505 @entity599
A novel, extremely thermophilic bacterium, designated strain 17S(T), was isolated from a deep-sea hydrothermal vent chimney at the Suiyo Seamount in the Izu-Bonin Arc, Japan. The cells were rods with no apparent motility, most of which were narrow in the middle in the exponential-growth phase and had @entity6734 at both ends. Growth was observed between 45 and 80 degrees C (optimum temperature, 70-75 degrees C; doubling time, 80 min) and between pH 5.0 and 7.0 (optimum pH, 5.4). The isolate was a strictly anaerobic chemolithoautotroph that was capable of using molecular @entity3884 as its sole energy source and @entity544 as its sole @entity505 source. Elemental @entity599 (S(0)) was required for growth as an electron acceptor. The G+C content of the genomic DNA was 34.6 mol%. Phylogenetic analysis based on 16S rDNA sequences indicated that the isolate was related to Thermovibrio ruber ED11/3LLK(T) and @entity70597 (T), whilst it appeared to be a novel lineage prior to the divergence of these genera. This isolate could also be differentiated from both @entity56261 ED11/3LLK(T) and @entity70597 (T) on the basis of physiological properties. The name Balnearium lithotrophicum gen. nov., sp. nov. is proposed for this isolate (type strain, 17S(T)=JCM 11970(T)=ATCC BAA-736(T)).
