[Distribution and efficiency of recombinant adenovirus mediated XXXX @entity8624 gene transfer in @entity19 with interstitial cells of Cajal loss].

@entity1 @entity19 @entity8624 @entity8302 @entity39004
OBJECTIVE: To investigate the construction of the green fluorescent protein (GFP) labeled recombinant adenovirus containing @entity1 @entity8624 ( @entity39004 ) and its distribution and efficiency in @entity19 with interstitial cells of Cajal (ICC) loss. METHODS: The recombinant adenovirus Ad-GFP/ @entity39004 was constructed by Ad-Easy system based on the homologous recombination in bacteria, then 1.6 x 10(9) PFU of recombinant adenoviruses were injected into Balb/c @entity19 with @entity8302 via the tail vein. In vivo distribution and efficiency of recombinant adenoviruses mediated @entity39004 were observed by GFP under the fluorescent microscope at different phases. The expression of @entity39004 gene was measured by RT-PCR method. The damages were observed in different organs by HE staining. RESULTS: The recombinant adenovirus containing @entity39004 was quickly constructed by homologous recombination in bacteria using Ad-Easy system. Under the fluorescent microscope, GFP was revealed in heart, lung, liver, spleen, kidney, small intestine and large intestine of @entity19 with @entity8302 at different phases. No obvious damages were observed in various visceral organs by HE staining. RT-PCR showed @entity39004 mRNA expression in various visceral organs at different levels. CONCLUSIONS: Construction of adenovirus vector by the homologous recombination in bacteria is an efficient and time saving method, and a high titer of adenovirus is able to mediate the safe and stable expression of @entity39004 gene in @entity19 with @entity8302 . This finding will make further gene therapy in @entity19 with STC possible.
