Inhibitory effects of the XXXX extract from bulbs of Scilla scilloides on lipoxygenase and hyaluronidase activities.

@entity32 @entity842 @entity6400 @entity1046
CONTEXT: Scilla scilloides Druce (Liliaceae) is a folk medicine to treat dermal @entity32 ; however, the medicinal properties of this plant have not been completely established. OBJECTIVE: The current study investigates the potent anti-inflammatory effects of S. scilloides bulbs for its traditional usage using lipoxygenase and hyaluronidase as the @entity32 model. To gain insight into the active constituents, nine homoisoflavones (1-9) were subsequently tested. MATERIALS AND METHODS: Lipoxygenase and hyaluronidase inhibition of @entity1046 extract from the bulbs of this plant within 2000     g/mL or homoisoflavones within 1000     M were determined by colorimetric methods. RAW264.7 cells were incubated with 10 or 50     M @entity6400 plus lipopolysaccharide (LPS) for 24   h. The culture media were collected and analyzed for determination of the @entity842 (NO) level by the colorimetric Griess method to measure the extent of @entity32 . RESULTS: The extract exhibited inhibitory effects on lipoxygenase and hyaluronidase activities with IC50 values 31.5 and 169     g/mL, respectively. Among the nine homoisoflavones tested, four (1 and 3-5) resulted in 79.3-97.9% higher lipoxygenase inhibition than 6.7-32.7% of the others at 500     M. Calculated IC50 values indicated 5 as the compound responsible for strong lipoxygenase inhibition with 15.8     M as the IC50 value. In the hyaluronidase assay, all homoisoflavones tested at 1000     M demonstrated 16.2-58.0% inhibition. Incubating the cells in the presence of all nine homoisoflavones tested at 50     M significantly suppressed the NO production, downward to 1.5-66.0%, in the LPS-activated macrophage cells as a model. DISCUSSION AND CONCLUSION: These results may indicate a potential role of S. scilloides for anti-inflammatory purposes.
