Monoclonal antibodies to @entity1 XXXX antigens demonstrated by immunofluorescence and immunoprecipitation.

@entity1 @entity4153 @entity5 @entity19 @entity957 @entity2262 @entity2 @entity1582 @entity2263 @entity420
Various studies have demonstrated the usefulness of monoclonal antibodies in recognizing discrete @entity5 antigenic determinants. The present study describes the tissue reactivity of monoclonal antibodies prepared against a @entity957 . Antigens were purified from the @entity5 extract by anti- @entity2262 affinity chromatography. These @entity2263 -associated antigens demonstrated @entity5 specificity by the leukocyte adherence inhibition assay. Antibody-secreting hybridomas were generated by fusion of @entity19 @entity1582 cells with @entity19 spleen cells immunized with purified @entity5 antigens. Hybridomas were selected by a solid-phase @entity5 membrane-binding immunoassay. The target specificity of the secreted monoclonal antibodies was ascertained by radioimmunoprecipitation analysis and indirect immunofluorescence on various @entity1 @entity5 and normal tissue sections. Monoclonal antibody-secreting hybridomas 48.4.8 and 48.4.2 secreted immunoglobulin that selectively immunoprecipitated polypeptide fragments from @entity1 @entity2 membrane antigens. Hybridoma 9.2.2 secreted antibody that was strongly positive by indirect immunofluorescence on all tested lung @entity957 . Adjacent or intervening normal lung tissue did not display significant immunofluorescence. @entity420 of the lung were negative or focally positive when focal squamous cell differentiation was present. @entity4153 were negative. The secreted antibody did not significantly stain three extrapulmonary @entity5 or a variety of normal tissues.
