Dentifrice pH but not consistency may affect XXXX uptake in plaque.

@entity6586 @entity460 @entity3282 @entity1388 @entity1098 @entity7034
OBJECTIVE: Test the ability of acidic fluoridated solutions to enhance @entity1388 (F) bound on bacteria (1) and the effect of dentifrice consistency on plaque fluid F uptake (2). METHODS: (1) @entity6586 isolates were grown in BHI medium (37  C/18h). Bacteria were washed either with @entity1098 or @entity3282 both at 1mM to remove or add @entity460 , respectively. Pellets were incubated with 12mM @entity7034 at pH 4.5 or 7 for 1min and F was quantified in the lysates and supernatants with the electrode, after HMDS-facilitated diffusion. (2) A randomized, double-blind, crossover clinical trial was performed in three phases with nineteen adults (20-35 years) that used one of the dentifrices: commercial toothpaste (1100ppm F, pH7.0 and conventional viscosity (Sorriso Fresh(  ))); experimental liquid dentifrice (ELD) (1100ppm F, pH7.0 and low viscosity [1.1% carboxymethylcellulose (CMC)]) and ELD (1100ppm F and high viscosity pH7.0 (2.2% CMC)). F concentration in plaque fluid was analyzed using an inverted F electrode. RESULTS: (1) Significantly higher F amounts were detected in the lysates of bacteria incubated with @entity7034 solution at pH4.5 compared to the supernatant, which was not seen at pH7.0, being this effect @entity460 -dependent. (2) Significantly higher F concentrations in plaque fluid were found 1h after toothbrushing compared to 12h, but no significant differences were seen among the toothpastes. CONCLUSIONS: F at low pH binds more efficiently to @entity6586 than at neutral pH and dentifrice viscosity does not interfere in plaque fluid @entity1388 incorporation. CLINICAL SIGNIFICANCE: pH of the dentifrice but not consistency may be important to F uptake in plaque.
