Acute uteroplacental ischemic embryo: @entity880 accumulation and prostaglandin production in the fetal XXXX brain.

@entity1438 @entity63 @entity1421 @entity2604 @entity880 @entity35 @entity3352
A new experimental model for studying the effects of acute @entity63 on brain development in the near-term fetal @entity35 has been devised. Ischemic conditions are achieved by complete clamping of blood vessels branching from the uterine vasculature into each individual fetus for designated times followed by removal of the clamps to permit reperfusion. Accumulation of @entity880 in the fetal brain depends on the length of the restriction period, reaching a plateau level of 29 mumol/g tissue at about 30 min. It also depends on the reperfusion time. Thus after a period of 15 min of restriction @entity880 levels show an increase over the next 30-min reperfusion to a value of 25.5 mumol/g followed by a rapid decrease to normal values by 3 h of reperfusion. Restriction of 15 min followed by reperfusion of 45 min causes an elevation of @entity1421 ( @entity1421 ) level from 12.4 +/- 0.86 ng/g to 21.1 +/- 0.6 ng/g (p less than 0.001). This elevation in @entity1421 level is less apparent after 20 min of restriction. No effects are seen on the level of PGF2 alpha. Both @entity1421 and PGF2 alpha accumulate in vitro in a time-dependent manner by brain particulate fraction. In vitro synthesis of both @entity1421 and PGF2 alpha is inhibited by @entity3352 (100% and 68%, respectively) and @entity1438 (94% and 76%, respectively). BW755c and @entity2604 do not affect @entity1421 formation but enhance PGF2 alpha production by 112% and 152%, respectively. Particulate fractions from restricted brain produce less PGF2 alpha than control brains (6.38 +/- 1.62 versus 11.43 +/- 2.2, p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
