microRNA-375 inhibits osteogenic differentiation by targeting XXXX .

@entity8087 @entity26261 @entity5 @entity13239 @entity623
UNASSIGNED: microRNAs (miRNAs) are involved in the regulation of almost all physiological and pathological processes, including osteoblast proliferation and differentiation. @entity26261 has been reported to be aberrantly expressed in various @entity5 types and to inhibit cell proliferation and invasion by targeting a number of key genes. However, the effects of @entity26261 on osteogenic differentiation have not been investigated. Thus, the present study aimed to elucidate the function served by @entity26261 in osteogenic differentiation. The expression levels of @entity26261 were observed to decrease in a C2C12 cell model of osteogenic differentiation. Overexpression of @entity26261 inhibited the activity of key osteoblast markers, including osteocalcin (OC), @entity623 ( @entity623 ) and collagen, type I, a 1 ( @entity13239 ). By contrast, inhibition of @entity26261 expression resulted in an increase in the osteogenic potential, as indicated by the enhanced expression levels of OC, @entity623 and @entity13239 . In addition, a dual-luciferase reporter assay indicated that @entity8087 ( @entity8087 ) was a target of @entity26261 . Western blot analysis revealed that the inhibition of @entity26261 led to a significant increase in the protein expression levels of @entity8087 . In addition, overexpression of @entity8087 was observed to attenuate the @entity26261 -mediated suppression of osteogenic differentiation. Therefore, the results demonstrated that @entity26261 was able to inhibit osteogenic differentiation via the regulation of @entity8087 expression.
