Induced pluripotent stem cells alleviate @entity651 from XXXX -reperfusion.

@entity1 @entity14231 @entity19 @entity409 @entity4613 @entity651 @entity2005 @entity7002 @entity204 @entity5327 @entity11838 @entity647
BACKGROUND: @entity5327 -reperfusion (I/R) injury is a serious pathophysiologic process that can trigger the development of @entity409 . @entity647 is a major cause of @entity204 among mesenteric I/R @entity1 , as current treatments remain inadequate. Stem cell-based therapies are considered novel strategies for treating several devastating and incurable diseases. This study examined whether induced pluripotent stem cells (iPSCs) lacking c-myc (i.e., induced using only the three genes @entity7002 , @entity14231 , and @entity2005 ) can protect against @entity647 in a mesenteric I/R @entity19 model. METHODS: C57BL/6 @entity19 were randomly divided into the following groups: sham/no treatment, vehicle treatment with @entity4613 -buffered saline, treatment with iPSCs, and treatment with iPSC-conditioned medium. The @entity19 were subjected to @entity5327 for 45 minutes followed by reperfusion for 24 hours. After I/R, the lungs and the ileum of the @entity19 were harvested. @entity651 was evaluated by histology, immunohistochemistry, and analyses of the levels of inflammatory cytokines, cleaved caspase 3, and @entity11838 . RESULTS: The intravenously delivered iPSCs engrafted to the lungs and the ileum in response to mesenteric I/R injury. Compared with the @entity4613 -buffered saline-treated group, the iPSC-treated group displayed a decreased intensity of @entity647 24 hours after mesenteric I/R. iPSC transplantation significantly reduced the expression of proinflammatory cytokines, oxidative stress markers, and apoptotic factors in injured lung tissue and remarkably enhanced endogenous alveolar cell proliferation. iPSC-conditioned medium treatment exerted a partial effect compared with iPSC treatment. CONCLUSION: When considering the anti-inflammatory, antioxidant, and antiapoptotic properties of iPSCs, the transplantation of iPSCs may represent an effective treatment option for mesenteric I/R-induced @entity647 .
