Characterization of a @entity35 model of XXXX @entity158 .

@entity263 @entity146 @entity5 @entity3478 @entity1711 @entity79 @entity1391 @entity1710 @entity158 @entity35 @entity9213 @entity4645
PURPOSE: The objectives of this study were to establish and characterize a novel animal model of @entity263 -induced @entity158 . METHODS: Copenhagen @entity35 were injected with 10(6) MATLyLu (MLL) @entity263 cells or @entity1391 -buffered saline by per cutaneous intra femoral injections into the right hind leg distal epiphysis. Over 13 days, @entity35 progressively developed a @entity5 within the distal femoral epiphysis. On days 3, 7, 10, and 13 post injection, @entity35 were subjected to the incapacitance and Randall-Selitto behavioral tests as they are believed to be indirect reflections of @entity5 induced @entity158 . Ipsilateral hind limbs were subjected to X-ray and computed tomography (CT) scans and histological sections were stained with @entity1710 and @entity1711 (H_E). RESULTS: Intra femoral injections of MLL cells resulted in the progressive development of a @entity5 leading to @entity79 and @entity146 . @entity5 development resulted in the redistribution of weight to the contralateral hind leg and significantly reduced the paw withdrawal threshold of the ipsilateral hind paw as observed via the incapacitance and Randall-Selitto tests, respectively. X-ray and computed tomography scans along with H_E stains indicated @entity5 -associated structural damage to the distal femur. This model was challenged with administration of @entity9213 . Compared with vehicle-injected controls, the @entity9213 -treated @entity35 displayed smaller nociceptive responses as observed with the incapacitance and Randall-Selitto tests, suggesting that @entity9213 was effective in reducing the @entity158 -related symptoms displayed by model animals and that the model behaved in a predictable way to @entity4645 treatment. CONCLUSIONS: This model is unique from other @entity3478 models in that it is a comprehensive model utilizing a competent immune system with a @entity5 . The model establishes a tool that will be useful to investigate mechanisms of @entity5 that are induced by @entity5 cells.
