[Microbiological evaluation of initial preparation for XXXX @entity1 ].

@entity1 @entity2520 @entity7278 @entity3288 @entity3841 @entity2523
The microbial flora from 46 @entity2520 of 23 @entity1 and 18 sites in 9 healthy @entity1 were examined and levels of serum IgG antibody to gram negative periodontal disease-associated bacteria were measured by enzyme-linked immunosorbent assay. Plaque samples and serum samples were taken 40-50 days after initial preparation consisting of scaling and root planing. To evaluate the effects of the therapy on 10 @entity1 with @entity7278 , changes in clinical parameters were compared with alterations of the microbial flora and serum IgG antibody levels. Black-pigmented Bacteroides species, mainly @entity2523 , were found to be predominant in @entity2520 . A significant relationship was found between the prevalence of @entity2523 and elevated titers of serum IgG antibody against the microorganism. No relationships between @entity3288 , @entity3841 and elevated titers of serum IgG antibody to them were detected. The fact that there was no marked reduction of serum IgG antibody to @entity2523 after initial preparation suggests that a more extended, longitudinal study is required. Although brushing resulted in a significant reduction of the number of total cultivable organisms in samples from periodontal pockets, no significant proportional changes in gram-negative bacteria in the lesional flora were found. Initial preparation was not effective in eliminating gram-negative bacteria from deep periodontal pockets. However, the microbiological shifts, especially the reduction in the proportion and frequency of detection of @entity2523 in periodontal pockets, was paralleled by significant improvement in the clinical parameters.
