Effect of diuretics on allergen-induced contractions of passively sensitized XXXX bronchi in vitro.

@entity1 @entity7130 @entity565 @entity1421 @entity1483 @entity30207 @entity1372 @entity6478 @entity18850 @entity9691 @entity11623 @entity3352 @entity26510
Inhaled @entity1483 has been shown to protect subjects with @entity565 from bronchoconstriction induced by a wide variety of stimuli, including allergen, but the mechanism of action is controversial. We have used an in vitro model of allergen-induced bronchoconstriction to examine the effects of @entity1483 and other ion transport inhibitors. @entity1 bronchial rings were passively sensitized by incubation with serum from an atopic donor and were challenged with @entity7130 . Allergen-challenged bronchial rings developed bronchoconstriction which was effectively inhibited by the @entity26510 antagonist @entity30207 (10(-7) M) and to a lesser extent by @entity9691 (10(-5) M). Assessed over 60 min @entity1483 10(-6), 10(-5), and 10(-4) M inhibited contractions by a mean (95% confidence interval [CI]) 7.9% (-23.5, 39.3%, p > 0.05), 44.2% (12.9, 75.2%, p < 0.01), and 86.9% (55.5, 118.3%, p < 0.001) respectively (n = 5). The same concentrations of @entity11623 inhibited contractions by 21.5% (-8.4, 51.4%, p > 0.05), 13.6% (-16.3, 43.4%, p > 0.05) and 51.6% (21.7, 81.4%, p < 0.01) respectively (n = 5). The @entity1372 transport inhibitor @entity6478 and the anion transport inhibitor @entity18850 ( @entity18850 ) were without effect (both 10(-4) M; n = 4). @entity1483 increased @entity1421 production by the bronchial rings by 134% (95% CI 53, 259%). @entity3352 (3 x 10(-6) M) blocked the @entity1483 -induced increase in @entity1421 production and reduced the protection afforded by 10(-4) M @entity1483 against allergen-induced contractions from 67.9% to 34.7% (mean difference 33.2%; 95% CI 9.7, 56.6%; p < 0.01; n = 8).(ABSTRACT TRUNCATED AT 250 WORDS)
