Extracorporeal Hypothermic Perfusion Device for Intestinal Graft Preservation to XXXX During Transportation.

@entity1 @entity1188 @entity63
INTRODUCTION: The small intestine is one of the most @entity63 -sensitive organs used in transplantation. To better preserve the intestinal graft viability and decrease @entity63 -reperfusion injury, a device for extracorporeal perfusion was developed. We present the results for the first series of perfused @entity1 intestine with an intestinal perfusion unit (IPU). METHODS: Five @entity1 intestines were procured for the protocol. (1) An experimental segment was perfused by the IPU delivering cold preservation solution to the vascular and luminal side continually at 4     C for 8 h. (2) Control (jejunum and ileum) segments were preserved in static cold preservation. Tissue samples were obtained for histopathologic grading according to the Park/Chiu scoring system (0   =   normal, 8   =   transmural @entity1188 ). RESULTS: Jejunal experimental segments scored 2.2 with the Park/Chiu system compared to the control segments, which averaged 3.2. Overall scoring for ileum experimental and control segments was equal with 1.6. CONCLUSION: This data presents proof of concept that extracorporeal intestinal perfusion is feasible. The evidence shows that the IPU can preserve the viability of @entity1 intestine, and histopathologic evaluation of perfused intestine is favorable. Our early results can eventually lead to expanding the possibilities of intestinal preservation.
