Immunosuppressive properties of cultured XXXX @entity267 and ciliary body in normal and pathological conditions.

@entity1 @entity267 @entity7499 @entity2372 @entity19 @entity1014 @entity3352 @entity320
Corneal transplantations may survive when transplanted orthotopically even in the presence of massive @entity7499 , while such grafts would be rejected in heterotopic locations. Experiments with @entity19 tissues have shown that cultured corneal tissue as well as iris/ciliary body (I/CB) tissue produce immunosuppressive factors. This was not the case when traumatized corneas were tested. We therefore wondered whether the same difference could be observed when explants of normal or pathological @entity1 corneas or I/CB tissues were tested. Supernatant of cultured @entity267 and I/CB tissue was added to a mixed lymphocyte reaction (BALB/c responder and C57B1/6 stimulator cells) and @entity1014 incorporation was measured. Culture supernatants from normal corneas and corneas with @entity2372 achieved similar levels of MLR inhibition, which could not be blocked by addition of anti-transforming-growth factor-beta 2 antibody or @entity3352 . The supernatant from cultured I/CB cells from normal eyes and from eyes containing a posterior segment @entity320 did not differ in their immunosuppressive effect. The MLR inhibitory effect of supernatant from I/CB cells was partly blocked by addition of @entity3352 . We conclude that MLR inhibiting factors are produced by normal and pathological corneas and by I/CB cells, but the supernatants from these tissues achieve their effect by different means. It is likely that the ability of @entity267 and I/CB tissues to produce immunosuppressive factors contributes to the success of orthotopic corneal transplants in @entity1 .
