Cellular pathways for rejection of class-I-MHC--disparate skin and XXXX allografts.

@entity8022 @entity5 @entity19 @entity22140 @entity3414 @entity1349
We have investigated the relative roles of the @entity3414 + and L3T4+ T lymphocyte subsets in rejection of class-I-MHC-antigen-disparate skin and @entity5 allografts. To deplete T cells in vivo, rat anti- @entity3414 or anti-L3T4 monoclonal antibodies (mAb) were administered to adult-thymectomized (ATX) recipient @entity19 prior to transplantation. BALB/c ( @entity8022 ) recipient @entity19 rejected the Ia- Sarcoma I (Sa1) (H-2a) tissue culture-derived @entity5 after depletion of the L3T4+ T cell subset in vivo. In contrast, depletion of the @entity3414 + T cell subset permitted @entity5 growth in all recipient @entity19 . To determine the role of particular T cell subsets in rejection of Ld class-I-MHC-antigen-disparate allografts, BALB/c skin was transplanted to BALB/c-H-2dm2 recipient @entity19 . Skin grafts were rejected by control @entity19 with a @entity1349 ( @entity1349 ) of 14.5 days. The @entity1349 of skin grafts for @entity19 treated with anti-L3T4 mAb was 16.6 days. In contrast, administration of anti- @entity3414 mAb alone ( @entity1349 = greater than 47 days) or together with anti-L3T4 mAb ( @entity1349 = greater than 50 days) caused prolonged or indefinite graft survival in all recipient @entity19 . Depletion of specific T cell subsets was confirmed by flow cytometric analysis and by analysis of T cell function in vitro. These results suggest that @entity3414 + T lymphocytes are essential for rejection of class-I-MHC-disparate allografts; indirect presentation of @entity22140 to L3T4+ T cells may not be necessary for rejection.
