Zerumbone protects XXXX @entity35 pancreatic beta cells from high glucose-induced apoptosis through generation of reactive oxygen species.

@entity2833 @entity137 @entity6 @entity2311 @entity6336 @entity13909 @entity35 @entity12220 @entity4854
The aim of this study is to explore the effect of zerumbone, a natural sesquiterpene isolated from Zingiber zerumbet Smith, on high glucose-induced @entity137 in pancreatic b cells. @entity2833 @entity35 pancreatic b cells were treated with 33 mM glucose with or without different concentrations of zerumbone and cell viability and apoptosis were assessed. The involvement of reactive oxygen species (ROS) and mitogen-activated protein kinase (MAPK) signaling in the action of zerumbone was examined. Notably, zerumbone significantly (P < 0.05) prevented the reduction of cell viability induced by high glucose. Such protection was in a concentration-dependent fashion up to 60  M of zerumbone. @entity13909 /propidium iodide staining analysis showed that zerumbone impaired the apoptotic response of high glucose-treated @entity2833 cells, which was coupled with a significant decline in cleaved caspase-3 and @entity2311 . Pretreatment with the ROS inhibitor N-acetylcysteine abrogated the phosphorylation of @entity12220 and @entity6336 induced by high glucose. Zerumbone significantly (P < 0.05) decreased the generation of ROS and the phosphorylation of @entity12220 and @entity6336 MAPKs in high glucose-treated @entity2833 cells. Pharmacological activation of @entity12220 and @entity6336 with anisomycin reversed the anti-apoptotic effect of zerumbone. Additionally, simultaneous inhibition of @entity12220 and @entity6336 significantly (P < 0.05) reduced the apoptotic response in high glucose-treated @entity2833 cells. In conclusion, zerumbone confers protection against high glucose-induced apoptosis of @entity2833 pancreatic b cells, largely through interfering with ROS production and @entity12220 and @entity6336 activation. Zerumbone may have potential therapeutic effects against @entity4854 -induced b cell damage in @entity6 .
