XXXX internal mammary artery organ culture model of coronary stenting: a novel investigation of smooth muscle cell response to @entity734 .

@entity1 @entity1373 @entity1311 @entity2945 @entity734
Local drug delivery by coronary stents is of current research interest. Organ culture of @entity1 vascular tissue is a model of intimal @entity1373 . We report an ex vivo organ culture model of stented vessels. This allows stent-artery interactions to be studied in living tissue. The recognized anti-restenosis agent @entity1311 was chosen to test the organ culture model. Mammary artery specimens were cultured 'closed' (i.e. without opening them flat) for 72 h. @entity2945 -coated stents, half of them loaded with the anti-restenosis drug @entity1311 , were implanted. The absorption and elution characteristics of @entity1311 were established. Artery tissue remained viable at 72 h when cultured closed, despite stent implantation. Specimens developed smooth muscle cell proliferation. The stents absorbed up to 127+/-29 microg of @entity1311 , with a biphasic elution curve. A mean of 13% of the absorbed @entity1311 remained after a 24 h perfusion. In mammary artery, these @entity1311 stents reduced or abolished smooth muscle cell proliferation compared with controls. This model allows the effects of stenting on @entity1 arterial tissue to be studied for at least 72 h, long enough to demonstrate effects on smooth muscle cell proliferation. @entity2945 -coated stents absorb adequate doses of @entity1311 , which is eluted gradually, inhibiting muscle cell proliferation. Such an organ culture model of stented mammary artery will provide useful data in addition to that from animal or cell culture models of @entity734 .
