Effect of XXXX and @entity3403 on IgE responses.

@entity748 @entity1 @entity1214 @entity1075 @entity2930 @entity2936 @entity3403 @entity3646 @entity803
BACKGROUND: We have recently found that the @entity803 @entity1214 suppresses inflammatory responses in serum immunoglobulin (Ig)E-positive @entity748 @entity1 , and that IgE levels can decrease in these @entity1 . The mechanism by which @entity1214 suppresses these responses is unknown. OBJECTIVE: We have now investigated the ability of the @entity2930 , @entity1214 and @entity3403 , to regulate IgE responses of peripheral blood mononuclear cells (PBMC) obtained from serum IgE-positive @entity748 @entity1 . METHODS: The distributions of CD3+, CD4+, CD8+, and @entity1075 + lymphocytes in peripheral blood of serum IgE-positive @entity748 @entity1 and IgE-negative nonasthmatic controls, and cytokine-specific mRNA expression by their PBMC were determined by flow cytometry (reverse transcriptase-polymerase chain reaction). Serum Ig levels also were determined (nephelometry, fluoroenzymeimmunoassay, enzyme-linked immunoadsorbent assay; n = 7/group). PBMC (1.5 x 10(6)/mL) were cultured with anti- @entity2936 monoclonal antibody and recombinant @entity1 @entity3646 in the presence/absence of @entity1214 or @entity3403 (0.1 to 10 microg/mL), and IgE levels in supernatants determined on days 0, 3, and 10 (enzyme-linked immunoadsorbent assay). RESULTS: Asthmatic and nonasthmatic subjects had similar numbers of blood CD4+ T cells (779/mm3 +/- 73 and 766 +/- 115, respectively) and @entity1075 + B-cells (239/mm3 +/- 35 and 379 +/- 95, respectively); however, CD8+ T cell numbers were decreased in @entity748 compared with nonasthmatic subjects (378/mm3 +/- 66 and 568 +/- 53, respectively; P = 0.045). High IgE levels were detected in supernatants of @entity748 PBMC on day 10 (28 ng/mL +/- 12), whereas control IgE levels did not change (<2.5 ng/mL). When either @entity1214 or @entity3403 was included in culture, IgE production by @entity748 PBMC was strongly suppressed in dose-dependent fashion on day 10 (>80% with 10 microg/mL); control IgE did not change (<2.5 ng/mL). CONCLUSIONS: The results are consistent with the idea that the therapeutic benefits obtained by @entity748 @entity1 from @entity1214 may, in part, result from IgE suppression.
