Peripheral injected @entity8586 -8S modulates the concentration of @entity750 in nerve fibers of the XXXX brainstem.

@entity3974 @entity6140 @entity5019 @entity8586 @entity750 @entity35 @entity18296 @entity1019
@entity750 and @entity8586 ( @entity6140 ) play a role in the short-term inhibition of food intake. It is known that peripheral injection of @entity6140 increases @entity5019 -immunoreactivity (Fos-IR) in the nucleus of the solitary tract (NTS) in @entity35 , and injection of the @entity750 antagonist @entity3974 decreases the number of @entity5019 -IR cells in the NTS. This supports the idea of @entity750 contributing to the effects of @entity6140 . The aim of the present study was to elucidate whether peripherally injected @entity6140 -8S modulates the concentration of @entity750 in brain feeding-regulatory nuclei. Ad libitum fed male @entity35 received 5.2 and 8.7 nmol/kg @entity6140 -8S (n=3/group) or 0.15M NaCl (n=3-5/group) injected intraperitoneally (ip). The number of @entity5019 -IR neurons, and the fluorescence intensity of @entity750 in nerve fibers were assessed in the paraventricular nucleus (PVN), @entity1019 ( @entity1019 ), NTS and dorsal motor nucleus of the vagus ( @entity18296 ). @entity6140 -8S increased the number of @entity5019 -ir neurons in the NTS (mean  SEM: 72  4, and 112  5 neurons/section, respectively) compared to vehicle-treated @entity35 (7  2 neurons/section, P<0.05), but did not modulate @entity5019 expression in the @entity18296 or @entity1019 . Additionally, @entity6140 -8S dose-dependently increased the number of @entity5019 -positive neurons in the PVN (218  15 and 128  14, respectively vs. 19  5, P<0.05). In the NTS and @entity18296 we observed a decrease of @entity750 -immunoreactivity 90 min after injection of @entity6140 -8S (46  2 and 49  8 pixel/section, respectively) compared to vehicle (81  8 pixel/section, P<0.05). No changes of @entity750 -immunoreactivity were observed in the PVN and @entity1019 . Our results suggest that @entity750 is involved in the mediation of @entity6140 -8's effects in the brainstem.
