@entity1199 activation of @entity137 T-lymphocytes infiltrating into XXXX metastatic @entity320 .

@entity1 @entity137 @entity5 @entity3460 @entity5041 @entity1199 @entity320
Tumor infiltrating lymphocytes (TIL) were isolated from 22 @entity5 obtained from 15 @entity1 with @entity320 . In 18 of the 22 @entity5 , a substantial number of lymphocytes was isolated with an average lymphoid cell: @entity5 cell ratio of 1.26. The TIL were predominantly @entity137 /suppressor T-lymphocytes with an average of 87% Leu4+, 61% Leu2a+, and 18% Leu3a+ cells. There were less than 2% natural killer cells, B-cells, or macrophages. An average of 3.8% (range, less than 0.1 to 8.6%) of freshly isolated TIL bound to @entity5 cells. Prior to culture, none of the @entity5 ( @entity5 ) was @entity137 as judged by @entity3460 exclusion. The frequency of @entity5 increased to 11.6% after 2 days of culture, and 10% of these @entity5 developed @entity137 activity. When @entity1199 was added to cultures, the frequency of @entity5 increased, and the frequency of @entity137 @entity5 was 2-fold higher compared to control cultures. After 10 days of culture with @entity1199 , TIL increased in number with a concomitant disappearance of @entity5 cells, whereas there were severe decreases of lymphocytes and no decrease of @entity5 cells in control cultures. TIL were cultured for 8 to 10 days with recombinant @entity1199 and tested for @entity137 against @entity5 cells and K562 targets in a 4-h 51Cr release assay. @entity5041 -cultured TIL from all nine @entity1 tested exhibited the highest levels of lysis against @entity5 cells. Of the nine TIL samples, five exhibited an apparent specificity for autologous @entity320 , while four specimens killed both allogenic and autologous @entity320 . The ability of TIL to kill K562 targets appeared to parallel the ability to kill allogenic targets. For comparison, recombinant @entity1199 -cultured peripheral blood mononuclear cells from the same @entity1 were assayed for @entity137 activity against @entity320 . Unlike some TIL, none of the peripheral blood mononuclear cells exhibited specificity for @entity5 cells. In summary, TIL isolated from @entity320 @entity1 were predominantly @entity137 T-lymphocytes with the ability to recognize and kill @entity5 cells after in vitro culture; @entity1199 induced proliferation of TIL and augmented their @entity137 activity such that they eliminated @entity5 cells.
