[Clinicopathological correlation of detection of regional lymph nodes micrometastasis in XXXX with @entity2 by reverse transcriptase-polymerase chain reaction].

@entity1 @entity4166 @entity5 @entity2 @entity3
BACKGROUND: To analyze clinicopathological correlation of detection of @entity2 micrometastasis in regional lymph nodes (LNs) by reverse transcriptase-polymerase chain reaction (RT-PCR). METHODS: Regional LNs (n=261) were obtained from 40 @entity1 with @entity2 who underwent lobectomy. Each LN was bisected. Half of each LN was subjected to histological examination (HE) and the other half was subjected to RT-PCR amplification of @entity4166 mRNA. RESULTS: In 18 of 40 @entity1 , the @entity3 in regional LNs was found by both HE and RT-PCR. Of 22 N0 diseases diagnosed by HE, 6 (27%) were found to express @entity4166 mRNA in regional LNs. According to the results of regional LNs @entity3 in 40 @entity1 by @entity4166 RT-PCR, the presence of the @entity4166 product in LNs was related to @entity5 size (Chi-square=5.76, P < 0.025), the presence of @entity5 vascular invasion (Chi-square=3.88, P < 0.05), cell differentiation of the @entity5 (Chi-square=7.08, P < 0.01) and P-TNM stages (Chi-square=7.42, P < 0.01). In the histologically node-negative @entity1 , lymph node micrometastasis was significantly related to @entity5 size (P=0.038, exact test) and P-TNM stage (P=0.01, exact test). However, the results of routine histological examination did not show the above clinicopathological correlation (P > 0.05). CONCLUSIONS: The RT-PCR method is superior to routine histological examination in detecting @entity3 in LNs. The micrometastasis in LNs can be detected accurately by RT-PCR. It is helpful for screening the @entity1 in whom the early subclinical @entity3 exists and disclosing the intrinsic rule of malignancy @entity3 .
