In vitro release of XXXX from collagen implants.

@entity1139 @entity1138 @entity1219
PURPOSE: To study @entity1138 Loaded Collagen Implant (CI) pharmacokinetics behaviour in vitro. METHODS: The CI were incubated for 15 minutes in different @entity1139 loading solutions with the following concentrations: 0.03 mg/mL (n = 9), 0.3 mg/mL (n = 10) and 3.0 mg/mL (n = 10). The loaded CI were transferred in 100 micro L of 0.9% @entity1219 . Aqueous flow of 5 micro L/min was simulated. The @entity1139 concentrations of the samples were determined by high performance liquid chromatography (HPLC). Dissolution kinetics were evaluated by a first-order process. The half-life of dissolution and the time of 95% dissolution were determined. RESULTS: The CI absorbed on average a @entity1139 dose of 0.054, 0.530 and 6.090 micro g when incubated in the different @entity1139 loading solutions containing 0.03 mg/mL, 0.3 mg/mL, 3.0 mg/mL of @entity1139 , respectively. In the release experiments, the mean total dose delivered by CI was 0.0493, 0.585 and 5.291 micro g. A linear correlation between loading concentration and the estimated total dose released was demonstrated. The kinetic parameters showed a fast @entity1139 dissolution. The half-life of the 3 series was 8.8, 10.1 and 10.5 min. CONCLUSIONS: Commercially available CI can be loaded with @entity1139 , and could provide relatively slower release than sponge delivery of @entity1139 . Clinical implications of these results warrants further studies.
