The role of the orphan nuclear receptor, liver receptor homologue-1, in the regulation of XXXX corpus luteum @entity1816 dehydrogenase type II.

@entity1 @entity2538 @entity1420 @entity1816 @entity2539 @entity8279 @entity39255 @entity25626
After ovulation, ovarian @entity1816 dehydrogenase type II ( @entity39255 ) expression increases to enhance the shift of steroidogenesis toward @entity1420 biosynthesis. @entity25626 ) is a transcription factor for several genes encoding steroidogenic enzymes. However, the level of @entity25626 expression decreases in the @entity1 corpus luteum (CL) after ovulation. @entity2538 ( @entity2538 ) is another member of the orphan nuclear receptor family. We hypothesize that @entity2538 , rather than @entity25626 , plays an essential role in the regulation of corpus luteum steroidogenesis. Semiquantitative RT-PCR and real-time PCR were performed to quantify the level of @entity2538 expression and correlate with @entity39255 level. Cell transfection, mutation analysis, and EMSA were performed to examine the role of @entity2538 in the regulation of @entity39255 . @entity2538 expression was higher in CL, compared with mature ovarian follicles. Cotransfection of granulosa cells with @entity39255 and @entity2538 resulted in a 10-fold increase of transcription. @entity2539 inhibited @entity2538 -stimulated @entity39255 , which was maintained in the presence of @entity8279 . Mutation of the either of the two putative @entity2538 binding sites, which were confirmed by EMSA, in the @entity39255 promoter decreased @entity2538 stimulation. Our findings suggest that @entity2538 is highly expressed in CL, and it plays an essential role in the regulation of @entity39255 .
