[Expression of inflammatory cytokines in a XXXX model of keratomycosis].

@entity19 @entity635 @entity10437 @entity5132 @entity281 @entity364 @entity9701 @entity777
OBJECTIVE: To investigate the expression, contribution and regulation of predominant inflammatory cytokines in the inflammation process of in a @entity19 model of keratomycosis. METHODS: It was an experimental study. Two hundred forty inbred BALB/c @entity19 were randomly divided into four groups (injury control group, @entity10437 infected group, A. fumigatus infected group and @entity5132 infected group) and establishment @entity364 was performed. At 1, 3, 5, and 7 days after @entity281 , the photographs of their corneas were taken using the slit lamp and the digital camera system. The presence of inflammatory cells and fungal hyphae were observed by HE and PAS staining. Four inflammatory cytokines in infectious corneas, including @entity9701 , KC, @entity635 , and @entity777 , were detected respectively for expression of mRNA and protein levels, using RT-PCR and ELISA. One-way ANOVA was used to test the comparisons of the clinical scoring, RT-PCR, and ELISA, and LSD was used to test multiple comparisons. RESULTS: The course and inflammatory reaction of corneal tissue in all infected group increased at day 3 and 5 and gradually decreased after day 7 with time. The RT-PCR results showed, the mRNA expression levels of @entity9701 and @entity635 were the highest in A. fumigatus infected group, and the expression levels of KC was the highest in @entity5132 infected group. Additionally, the expression levels of @entity777 were found to be the highest in @entity10437 infected group. The mRNA expression levels of @entity635 and KC in each experimental group were higher than those of @entity777 and @entity9701 . The peak expression levels of @entity9701 , KC, and @entity635 occurred at day 5, day 1, and day 3 in @entity10437 infected group, A. fumigatus infected group and @entity5132 infected group, respectively. However, the peak expression level of @entity777 occurred at day 1 and 7, day 1 and 7, and day 1 and 5 in the @entity10437 , A. fumigatus, and @entity5132 groups, respectively. The difference of mRNA expression of each cytokine had statistically significant between each groups ( @entity9701 : F = 1675.339, P < 0.01; KC: F = 730.267, P < 0.01; @entity635 : F = 297.106, P < 0.01; @entity777 : F = 174.513, P < 0.01). The protein expression levels of the four cytokines in the infected groups from high to low in turn were @entity635 , @entity9701 , KC and @entity777 . The trend of protein expression of the four cytokines at each time point was the same as the cytokine mRNA expression in different experimental groups as well as within the same group. The difference of protein expression of each cytokine had statistically significant between each groups ( @entity9701 : F = 2871.736, P < 0.01; KC:F = 886.598, P < 0.01; @entity635 : F = 3595.488, P < 0.01; @entity777 : F = 89.225, P < 0.01). CONCLUSIONS: The expression levels of @entity9701 , KC and @entity635 are closely related to the performance of the disease severity and inflammatory reaction of FK. The expression of @entity9701 and @entity635 may play important role in the pathogenesis of FK.
