In vivo local expansion of clonal T cell subpopulations in XXXX .

@entity1 @entity1358 @entity2612 @entity5
@entity1358 ( @entity1358 ) is one @entity1 @entity5 to which the immune response may control the growth of @entity5 cells. These @entity5 are infiltrated by a large mononuclear infiltrate mainly composed of T lymphocytes. To characterize the lymphocytes infiltrating @entity1358 , we analyzed the molecular structure of the T cell receptor (TCR) alpha and beta chains in @entity5 and paired peripheral blood lymphocytes from a series of 6 untreated @entity1 . We first determined V alpha and V beta gene segment usage by PCR using a panel of V specific oligonucleotide primers (V alpha 1-w29 and V beta 1-w24). A highly diverse usage of TCR V alpha and V beta gene usage was observed in 5 of 6 @entity5 . In addition, the few @entity5 overexpressed V beta specificities detected by reverse transcription-PCR were shown to contain minor T cell expansions. Strikingly, 1 of the 6 @entity5 studied displayed a skewed TCR repertoire with V beta 4 transcript representing 25% of the TCR signals. @entity5 overexpressed V beta transcripts was analyzed by @entity2612 size distribution analysis. In the particular @entity5 displaying a biased repertoire large expansions of T cell subpopulations were detected (particularly in V beta 4) specifically at the @entity5 site. Such T cells may be expanded locally in response to @entity5 antigens.
