Antimicrobial effect of photodynamic therapy in XXXX in vivo, using culture and real time PCR methods.

@entity6586 @entity1547 @entity6693
BACKGROUND: The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) in @entity1547 in vivo by culture and real-time PCR methods. METHODS: Ten teeth with deep active @entity1547 were selected and five portions of carious dentin were removed for each tooth. Two increments were used as control, to represent the superficial and deep dentin, respectively. @entity6693 at 100mg/L was placed in contact with the cavity for five minutes, before being irradiated with a halogen light source for one minute. Then, after PDT, other three portions were removed. The samples were processed in laboratory and the number of viable cfu was obtained. The real time PCR analyses were performed in two increments of carious dentin, removed before and after PDT. The @entity6586 DNA was isolated from carious dentin samples and amplification and detection of DNA were performed with Real-Time PCR. The cavities were then restored with glass ionomer cement. RESULTS: Using conventional culture methods, the results demonstrated that viable bacteria were significantly reduced in all of the agar plates following photosensitization. No difference was found between both groups regarding @entity6586 DNA quantification by real-time PCR. CONCLUSION: Although PDT may not affect the number of @entity6586 DNA copies immediately after the treatment, clear reduction of the number of cfu was found. Despite its promising use for eliminating bacteria in dental caries treatment, further studies are necessary to establish an effective clinical protocol for the PDT.
