Autologous cardiomyotissue implantation promotes myocardial regeneration, decreases XXXX size, and improves left ventricular function.

@entity5045 @entity1707 @entity11017 @entity1188 @entity11018 @entity35 @entity583
BACKGROUND: Cell therapy for @entity583 ( @entity583 ) may be limited by poor cell survival and lack of transdifferentiation. We report a novel technique of implanting whole autologous myocardial tissue from preserved myocardial regions into infarcted regions. METHODS AND RESULTS: Fourteen @entity35 were used to optimize cardiomyotissue size with peritoneal wall implantation (300  m identified as optimal size). Thirty-nine @entity1707 were used to investigate cardiomyotissue implantation in @entity583 induced by left anterior descending balloon occlusion (10 animals died; male-to-female transplantation for tracking with in situ hybridization for Y chromosome, n=4 [2 donors and 2 @entity583 animals]; @entity583 at 1 hour, n=13; and healed @entity583 implantation at 2 weeks, n=12). Assessment included echocardiography, magnetic resonance imaging, hemodynamics, @entity5045 staining, and histological and molecular analyses. Tracking studies demonstrated viable implants with donor cells interspersed in the adjacent myocardium with gap junctions and desmosomes. In the @entity583 , treated animals compared with controls had improved perfusion by magnetic resonance imaging (1.2  0.01 versus 0.86  0.05; P<0.01), decreased @entity583 size (magnetic resonance imaging: left ventricle, 2.2  0.5% versus 5.4  1.5%, P=0.04; @entity5045 : anterior wall, 10.3  4.6% versus 28.9  5.8%, P<0.03), and improved contractility (dP/dt, 1235  215 versus 817  817; P<0.05). In the healed @entity583 cohort, treated animals had less decline in ejection fraction between 2 and 4 week assessment (-3  4% versus -13  -4%; P<0.05), less decline in   dP/dt, and smaller @entity583 ( @entity5045 , 21  11% versus 3  8%; P=0.006) than control animals. @entity1188 in the treated animals contained more mdr-1(+) cells and fewer c-kit(+) cells with a trend for decreased expression of @entity11017 and increased expression of @entity11018 . CONCLUSION: Autologous cardiomyotissue implanted in an @entity583 area remains viable, exhibits electromechanical coupling, decreases @entity1188 size, and improves left ventricular function.
