Uncoupling of XXXX Complex Receptor during In Vitro Aging Is Related to Modifications in Its Intrinsic Sialidase Activity and the Subsequent @entity18530 Production.

@entity18530 @entity689 @entity1 @entity2362 @entity5132 @entity18531 @entity1366
UNASSIGNED: Degradation of @entity2362 leads to the production of @entity2362 -derived peptides (EDP), which exhibit several biological effects, such as cell proliferation or protease secretion. Binding of EDP on the @entity2362 receptor complex (ERC) triggers @entity18530 ( @entity18531 ) production and @entity1366 activation following ERC @entity689 subunit activation. The ability for ERC to transduce signals is lost during aging, but the mechanism involved is still unknown. In this study, we characterized an in vitro model of aging by subculturing @entity1 dermal fibroblasts. This model was used to understand the loss of EDP biological activities during aging. Our results show that ERC uncoupling does not rely on @entity689 or @entity5132 mRNA or protein level changes. Furthermore, we observe that the membrane targeting of these subunits is not affected with aging. However, we evidence that @entity689 activity and @entity18531 production are altered. Basal @entity689 catalytic activity is strongly increased in aged cells. Consequently, EDP fail to promote @entity689 catalytic activity and @entity18531 production in these cells. In conclusion, we propose, for the first time, an explanation for ERC uncoupling based on the age-related alterations of @entity689 activity and @entity18531 production that may explain the loss of EDP-mediated effects occurring during aging.
