Humoral defense of the XXXX Ascaris suum: antibacterial, bacteriolytic and agglutinating activities in the body fluid.

@entity1 @entity2199 @entity4059 @entity3584 @entity794 @entity10130 @entity7178 @entity3881 @entity526
Three humoral defense activities (antibacterial, bacteriolytic and agglutinating) were detected in the body fluid of the @entity2199 @entity10130 . Gram-positive bacteria ( @entity794 and @entity3881 ) were more sensitive to the antibacterial activity than the Gram-negative bacteria ( @entity526 coli). The antibacterial activity was heat stable and was lost by trypsin digestion. The molecular mass of the factor responsible for antibacterial activity was estimated as 6 kDa. The bacteriolytic activity against dried @entity7178 was also detected. The bacteriolytic factor was 6-9 kDa in molecular mass, heat sensitive and trypsin sensitive. Both @entity526 and @entity3584 -fixed trypsin-treated @entity1 A-type red blood cells were agglutinated in the body fluid. An analytical gel permeation HPLC revealed the agglutinating activity consists of at least two @entity4059 . Activities of both agglutinating @entity4059 were lost by heat treatment or trypsin digestion. The molecular masses estimated for the two agglutinating @entity4059 were 500 kDa and 25 kDa. Under experimental conditions, microbe-injection was not a prerequisite for the appearance of these defense activities.
