Use of a novel XXXX fibre composite material for the femoral stem component of a THR system: in vitro biological assessment.

@entity1 @entity13843 @entity273 @entity12316 @entity920 @entity505
A novel, low elastic modulus femoral component for THR has been developed using a composite of @entity12316 and @entity505 fibre. The objectives of this study were to investigate @entity1 osteoblast-like cell and macrophage responses to this material in vitro. Cells were grown on composite discs and controls. Osteoblast attachment and proliferation was not significantly different to that on @entity13843 . The levels of alkaline phosphatase activity, Type I collagen production and osteocalcin production were not significantly different to that on @entity13843 by the end of the experimental period. @entity273 production by macrophages was significantly less than that detected for cells cultured on @entity920 , but was still greater than that detected for cells cultured on tissue culture plastic and @entity13843 . Beta-glucoronidase activity was not significantly different to that detected for cells cultured on tissue culture plastic. The in vitro biocompatibility assessment of this composite undertaken in this study showed initial osteoblast attachment at least comparable to that of the tissue culture plastic and @entity13843 controls, with proliferation similar to the controls at all time points up to 11 days. Alkaline phosphatase activity was similar to that of @entity13843 but reduced compared to tissue culture plastic controls. Whilst @entity273 production by macrophages was raised on composite surfaces compared to controls, beta-glucoronidase activity and osteoblastic production of Type I collagen and osteocalcin were similar to levels detected on @entity13843 .
