An @entity70660 is required for @entity70658 regulatory subunit localization and morphology of actin structures during oogenesis in XXXX .

@entity70658 @entity51701 @entity43183 @entity70659 @entity669 @entity238 @entity70660
@entity70658 ( @entity70658 ) holoenzyme is anchored to specific subcellular regions by interactions between regulatory subunits ( @entity70658 -R) and A-kinase anchoring proteins (AKAPs). We examine the functional importance of @entity70658 anchoring during @entity238 oogenesis by analyzing membrane integrity and actin structures in mutants with disruptions in @entity70659 , an @entity70660 . In wild-type ovaries, @entity70658 and @entity70659 localized to membranes and to the outer rim of ring canals, actin-rich structures that connect germline cells. In @entity70659 mutant ovaries, @entity70658 membrane localization decreased, leading to a destabilization of membrane structures and the formation of binucleate nurse cells. Defects in membrane integrity could be mimicked by expressing a constitutively active @entity43183 ( @entity43183 ) throughout germline cells. Unexpectedly, nurse cells in @entity70659 mutant ovaries also had enlarged, thin ring canals. In contrast, overexpressing @entity70659 in the germline resulted in thicker, smaller ring canals. To investigate the role of @entity70659 in regulating ring canal growth, we examined genetic interactions with other genes that are known to regulate ring canal morphology. @entity70659 mutations suppressed the small ring canal phenotype produced by @entity51701 mutants, linking @entity70659 with the non-receptor @entity669 kinase pathway. Together, these results provide the first evidence that @entity70658 localization is required for morphogenesis of actin structures in an intact organism.
