[Experimental study of the effect of liposome-mediated vascular endothelial growth factor gene on the flap survival in XXXX ].

@entity35 @entity6667
OBJECTIVE: To investigate the effect of @entity6667 gene on the random flap survival after pedicle division at different time in @entity35 . METHODS: The random-pattern flaps were formed on the back of the 120 SD @entity35 . PcDNAVEGF165 (gene) wrapped with liposome was injected into the flaps in experimental group (n = 40). The flaps in the two control groups were injected with PcDNA (n = 40) or saline (n = 40). 1, 3, 5, 7 days after injection, 10 @entity35 in each group were randomly selected to performed pedicle division. 7 days after pedicle division, the @entity35 were killed to measure the flap survival rate. The microvessels was studied by histologic examination. The expression of @entity6667 was assessed by immunohistochemical staining. The flaps were also examined under the electron ultrastructure microscopy. RESULTS: 1) Flap survival rate after pedicle division in experimental group at 1 day, 3 days, 5 days, 7 days after injection, were (45.45 +/- 12.24)%, (82.95 +/- 3.81)%, (85.00 +/- 3.38)%, (85.96 +/- 3.25)%, respectively. The flap survival rates were significant different between experimental group and the control groups at 3, 5, 7 days after injection (P < 0.05), but not at 1 days after injection (P > 0.05). 2) The average microvascular diameter and number in experimental group were significantly higher than those in control groups (P < 0.05). 3) The expression of @entity6667 in experimental group was significantly higher than that in the two control groups (P < 0.05). 4) Ultrastructure study showed more angiogenesis in experimental group. CONCLUSIONS: Subcutaneous injection of liposome-mediated @entity6667 gene can increase the survival rate of flap with early pedicle division. It is a simple, efficient, economic, and the relatively safe gene therapy.
