[Identification of @entity72904 in the XXXX brain and its relevancy to ischemic cerebral edema].

@entity2657 @entity63 @entity75 @entity4 @entity2500 @entity72904 @entity11117 @entity35 @entity1813
@entity1813 are thought to be important in the pathogenetic mechanism of @entity63 @entity75 . But little is known about lipoxygenase products and their roles in brain. In the present study, lipoxygenase metabolism in the brain and its relevancy to @entity63 @entity2657 were studied using high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The @entity35 middle cerebral artery (MCA) occlusion model was used because the time course of @entity63 @entity4 formation has been well known. The @entity35 brain was fixed by in situ freezing 24 and 72 hours after @entity2500 , and removed. Identification and quantitative analysis of @entity11117 (HETEs) in normal and @entity63 brain homogenates was performed by HPLC and GC-MS. The @entity35 brain was divided into the microvessel (MV) fraction and the rest. Then the same analysis was carried out in the both fractions obtained from the normal @entity35 brain. Only @entity72904 was detected in the normal and @entity63 brain. Quantitatively, normal brains contained 1288 +/- 66 (mean +/- SE) of @entity72904 ng/g wet weight, while the hemispheres rendered @entity63 for 24 and 72 hours after @entity2500 contained 1101 +/- 48, and 1663 +/- 147, respectively. The @entity72904 content was significantly increased 72 hours after @entity2500 (p less than 0.05). The MV fraction of @entity35 brain contained @entity72904 (653 ng/mg protein) ten times as much as the other fraction. The identification of @entity72904 in the @entity35 brain is a new finding.(ABSTRACT TRUNCATED AT 250 WORDS)
