Experience of more than 100 preimplantation genetic diagnosis cycles for XXXX using whole genome amplification and linkage analysis in a single centre.

@entity1 @entity249 @entity66 @entity5 @entity179 @entity74
OBJECTIVE: To report the outcomes of more than 100 cycles of preimplantation genetic diagnosis for @entity66 . DESIGN:                 <AbstractText Label="CASE SERIES" NlmCategory="METHODS"/>                <AbstractText Label="SETTING" NlmCategory="METHODS">Tertiary assisted reproductive centre in Hong Kong, where @entity1 needed to pay for the cost of preimplantation genetic diagnosis on the top of standard in-vitro fertilisation charges. @entity1 : @entity1 undergoing preimplantation genetic diagnosis for @entity66 at the Centre of Assisted Reproduction and Embryology, Queen Mary Hospital-The University of Hong Kong between 1 August 2007 and 30 April 2014 were included. INTERVENTIONS: In-vitro fertilisation, intracytoplasmic sperm injection, embryo biopsy, and preimplantation genetic diagnosis. MAIN OUTCOME MEASURES: Ongoing pregnancy rate and implantation rate. RESULTS: Overall, 124 cycles of preimplantation genetic diagnosis were initiated in 76 @entity1 , 101 cycles proceeded to preimplantation genetic diagnosis, and 92 cycles had embryo transfer. The ongoing pregnancy rate was 28.2% per initiated cycle and 38.0% per embryo transfer, giving an implantation rate of 35.2%. There were 16 frozen-thawed embryo transfer cycles in which, following preimplantation genetic diagnosis, cryopreserved embryos were replaced resulting in an ongoing pregnancy rate of 37.5% and implantation rate of 30.0%. The cumulative ongoing pregnancy rate was 33.1%. The most frequent indication for preimplantation genetic diagnosis was thalassaemia, followed by @entity179 and @entity5 predisposition. There was no misdiagnosis. CONCLUSIONS: Preimplantation genetic diagnosis is a reliable method to prevent couples conceiving fetuses severely affected by known @entity74 , with ongoing pregnancy and implantation rates similar to those for in-vitro fertilisation for routine @entity249 treatment.
