Lymphocytes infiltrating the CNS during @entity32 display a distinctive phenotype and bind to XXXX but not to @entity37196 .

@entity32 @entity198 @entity19 @entity784 @entity4459 @entity6580 @entity1651 @entity37196 @entity8024 @entity3804 @entity3844 @entity6232
The nature of inflammatory lymphocytes recruited to the CNS has been studied in a model of @entity32 . Injection of killed Corynebacterium parvum into the cortex of the @entity19 brain produces a circumscribed inflammatory cellular infiltrate around the injection site, and recruited mononuclear inflammatory cells (IC) can be isolated for flow cytometric analysis. The majority of IC were T cells. In comparison with the predominant naive population of mesenteric lymph node T cells, @entity784 cells express much higher levels of @entity3844 , @entity4459 and @entity8024 , and lower levels of CD45RB, features commonly associated with memory (previously activated) cells. In addition, in contrast to the @entity6232 + alpha 6-integrinlow phenotype of naive lymph node T cells, @entity784 cells lacked @entity6232 and were alpha 6-integrin-. @entity3804 , recently proposed as another marker of memory T cell differentiation, was not displayed by @entity784 cells, suggesting that @entity3804 expression may be heterogeneous among memory T cell subsets. A subset of mesenteric lymph node (MLN) T cells, probably representing activated T cells undergoing the naive to memory transition, but not of @entity784 cells, expressed high levels of alpha 6-, beta 7- and alpha E-integrin. IC and MLN naive T cells expressed comparable levels of alpha 4-integrin, but @entity784 cells stain poorly with anti-beta 7 mAbs and with mAb DATK 32, specific for the alpha 4 beta 7 heterodimeric lymphocyte homing receptor for the mucosal addressin @entity37196 , suggesting that these inflammatory cells express more alpha 4 beta 1 than alpha 4 beta 7. Consistent with this, in in vitro adhesion assays, @entity198 bound better than MLN cells to the alpha 4 beta 1 integrin ligand @entity6580 and the @entity4459 ligand @entity8024 but adhered very poorly to the alpha 4 beta 7 ligand @entity37196 . These findings are consistent with and extend previous immunohistological studies of T cells in @entity19 experimental autoimmune @entity1651 , and demonstrate a distinctive phenotype for lymphocytes being present in the chronically inflamed brain.
