Behavioral and immunohistochemical changes in an experimental XXXX model in @entity35 .

@entity32 @entity2227 @entity1931 @entity625 @entity10133 @entity4563 @entity367 @entity35 @entity4655
An experimental @entity625 induced by injection of @entity4563 and carrageenan into the knee joint resulted in a temporal relationship between @entity2227 dorsal horn content and paw withdrawal latency (PWL) which was positively correlated. Limping, guarding, increased response to heat stimuli ( @entity1931 ) and altered staining patterns for @entity2227 ( @entity367 ), substance @entity10133 ), and calcitonin gene-related peptide ( @entity4655 ) were monitored in the awake behaving arthritic @entity35 over a 1 week time course. A decrease in PWL occurred on the side ipsilateral to the inflamed knee as early as 4 h after the induction of @entity625 indicating the animals are @entity1931 . The PWL remained decreased through the first 24 h. Computer-assisted quantification of the density of immunohistochemical staining indicated the content of @entity367 , SP and @entity4655 was altered differentially throughout the time course of the @entity625 . The changes observed for all three substances occurred across the entire superficial dorsal horn. There was an initial depletion of SP followed by an increase in both SP and @entity4655 content which was maintained through 1 week. The @entity367 content was increased during the @entity1931 period. The @entity367 changes followed the same time course and were positively correlated with the changes in PWL. In a small group of animals injected with @entity4563 and carrageenan, @entity1931 did not develop. In this group of animals, no change in dorsal horn @entity367 or SP content occurred. Rather, there was an increase in @entity4655 content in the middle portion of the superficial dorsal horn which is the termination site of knee joint afferents. These data indicate that the development of @entity1931 is dependent on @entity367 and possibly SP. Since @entity32 of the knee joint does not involve the foot pad, the @entity1931 observed during the first 24 h following induction of @entity625 represents a central neuronal sensitization.
