Surface modification of XXXX with biomolecules to promote endothelialization.

@entity1 @entity15274 @entity3678 @entity7049 @entity715 @entity1473
Rapid endothelialization is important for biodegradable blood-contacting devices not only to prevent @entity715 but also to prevent degradation debris from entering the bloodstream and causing further complications. Here the authors report a three-step surface modification method, by which biomolecules, such as gelatin and chitosan, are covalently immobilized on the surface of plasma-treated @entity7049 ( @entity15274 ) via - @entity1473 groups introduced by @entity3678 grafting polymerization. Surface characterization techniques, including x-ray photoelectron spectroscopy, contact angle measurement, and colorimetric methods for surface density of functional groups, proved the feasibility and stability of this surface modification method. Surface wettability was increased by biomolecules immobilization. The - @entity1473 surface density was measured to be 4.17  0.15    mol/cm(2), the and amount of gelatin immobilized was 4.8    g/cm(2). @entity1 umbilical vein endothelial cell was used during in vitro study at seeding density of 10(4)   cells/cm(2). @entity15274 -gAA-gelatin surface was found to enhance cell adhesion, spreading, focal adhesion formation, and proliferation significantly. Chitosan-modified @entity15274 shows marginally improvement in cell adhesion and proliferation. Endothelialization was achieved within 7 days on both modified @entity15274 surfaces. In conclusion, this work demonstrates the feasibility of the surface modification method, and its ability to promote complete endothelialization for cardiovascular applications.
