sLeX/ XXXX mediates adhesion in vitro implantation model.

@entity1 @entity1196 @entity8729 @entity23521
The complex implantation process is initiated by the recognition and adhesion between the embryo and uterine endometrial epithelium. The expression and interactions between the adhesive molecules from both fetal and maternal sides are crucial for the successful implantation. In this study, we aimed to investigate the expression and adhesive function of sLeX on the trophoblasts and @entity1196 on uterine epithelial cells mediated the adhesion at the fetal-maternal interface, and to further explore whether this adhesion system could induce endometrial apoptosis, using in vitro implantation model consisting of the @entity1 trophoblast cell line (JAR) and @entity1 uterine epithelial cell line (RL95-2). The results showed that sLeX was expressed on JAR cells by indirect immunofluorescence staining. After transfection of JAR cells with @entity23521 ( @entity23521 ) which is the key enzyme for sLeX synthesis, the expression of @entity23521 and sLeX synthesis were increased, and the percent adhesion of trophoblast cells to @entity1 cell monolayer was significantly increased (P  <  0.01). @entity1196 was strongly expressed but not E- and @entity8729 on epithelial @entity1 cells by RT-PCR, Western blot. Blocking @entity1196 with specific antibody or heparin pretreatment in @entity1 cells inhibited the adhesion of JAR cells to @entity1 cell monolayer. Furthermore, regulating the expression of sLeX on JAR cells or blocking @entity1196 on @entity1 cells could activate the apoptosis of uterine epithelial cells. These results suggest the sLeX/ @entity1196 adhesion system at fetal-maternal interface not only mediates the adhesion of embryo to uterine epithelium, but also effectively induces the apoptosis in uterine epithelium. The study supplies a molecular basis for the elucidation of the initial recognition and adhesion during embryo implantation.
