[Immune response elicited by the recombinant protein and plasmid DNA of complex antigen ROP2-SAG1 from XXXX ].

@entity2047 @entity19 @entity9686 @entity60 @entity9749 @entity3204
OBJECTIVE: To study the immune response elicited by the recombinant protein vaccine and DNA vaccine of the complex antigen ROP2-SAG1 from @entity3204 . METHODS: Sixty female BALB/c @entity19 were randomly divided into 4 groups (15 per group). @entity19 in rROP2-SAGI group were immunized subcutaneously with 2.5 microg rROP2-SAG1 protein formulated in Freund's adjuvant. @entity19 in control group received only adjuvant emulsified with normal saline. @entity19 in recombinant plasmid pcROP2-SAG1 and control plasmid pcDNA3.1 groups were each injected intramuscularly with 100 microg of pcROP2-SAG1 and pcDNA3.1, respectively. All the @entity19 received three immunizations at 2-week intervals. Serum samples were collected at 25, 45, and 70 days after immunization for determining antibody IgG, and at 2 weeks after the last immunization @entity9686 and @entity9749 were detected all by ELISA. Cell counting kit-8 (CCK-8) was used to determine the splenocyte proliferation, and the supernatant of cultured splenocytes was collected for the detection of @entity60 by ELISA. RESULTS: The level of IgG continued to rise in rROP2-SAG1 group after immunization, and similarly in pcROP2-SAG1 group. At 2 weeks after the last immunization, level of @entity9686 (1.538 +/- 0.183) was higher than that of @entity9749 (0.618 +/- 0.122) (P < 0.05) in rROP2-SAG1 group. Whereas no significant difference between @entity9686 (1.107 +/- 0.137) and @entity9749 (0.830 +/- 0.185) was observed in pcROP2-SAG1 group (P > 0.05). Compared with the pcROP2-SAG1 group (A450 = 0.123 +/- 0.018), more significant proliferation response of splenocytes was observed in rROP2-SAG1 group (0.348 +/- 0.042) (P < 0.05). There was no significant difference (P > 0.05) of @entity60 and @entity2047 in the supematant of cultured splenocytes between the groups of rROP2-SAG1 and pcROP2-SAG1. CONCLUSION: The antibody level and splenocyte proliferation have been significantly higher in @entity19 immunized with recombinant protein rROP2-SAG1 than those with recombinant plasmid pcROP2-SAG1.
