Enhanced Humoral Responses Induced by Targeting of Antigen to XXXX Dendritic Cells.

@entity68823 @entity65396 @entity19 @entity4302 @entity72212 @entity13384 @entity39379 @entity11805
UNASSIGNED: Dendritic cells (DCs) are superior in their ability to induce and control adaptive immune responses. These qualities have motivated the hypothesis that targeted delivery of antigen to DCs in  vivo may be an effective way of enhancing immunization. Recent results show that antigen targeted to certain DC surface molecules may indeed induce robust immune responses. Targeting of antigen to DCs can be accomplished by the means of monoclonal antibodies. This study compared the humoral responses induced in @entity19 by in  vivo targeting of DCs using monoclonal antibodies specific for @entity11805 , @entity4302 , @entity13384 , @entity65396 , @entity39379 , @entity68823 , @entity72212 and PDC-TREM. The results demonstrate that antigen delivery to different targets on DCs in  vivo gives rise to humoral responses that differ in strength. Targeting of antigen to @entity11805 , @entity4302 , @entity13384 , @entity65396 , @entity39379 and PDC-TREM induced significantly stronger antibody responses compared to non-targeted isotype-matched controls. Targeting of @entity68823 and @entity72212 did not lead to efficient antibody responses, which may be due to unfavourable properties of the targeting antibody, in which case, other antibodies with the same specificity might elicit a different outcome. Anti- @entity11805 was additionally used for elucidating the impact of the route of vaccination, and the results showed only minor differences between the antibody responses induced after immunization either s.c., i.v. or i.p. Altogether, these data show that targeting of different surface molecules on DCs result in very different antibody responses and that, even in the absence of adjuvants, strong humoral responses was induced.
