Recombinant XXXX Elastase Alters the Compliance of Atherosclerotic Tibial Arteries After Ex Vivo Angioplasty.

@entity1 @entity2362 @entity3657 @entity13403 @entity384 @entity2701 @entity731
PURPOSE: This study was designed to determine whether vonapanitase (formerly PRT-201), a recombinant @entity1 elastase, treatment can fragment the protein @entity2362 in elastic fibers and cause dilation of @entity731 @entity1 peripheral arteries subjected to ex vivo balloon angioplasty. MATERIALS AND METHODS: Seven @entity1 undergoing lower limb amputation for @entity3657 or who died and donated their bodies to science donated 11 tibial arteries (5 anterior, 6 posterior) for this study. All arteries were @entity731 by visual inspection. The arteries underwent ex vivo balloon angioplasty and thereafter were cut into rings and studied on wire myographs where the rings were stretched and @entity2701 was recorded. After treatment with vonapanitase 2 mg/mL or vehicle control, myography was repeated and the rings were then subject to @entity2362 content measurement using a @entity13403 radioimmunoassay and elastic fiber visualization by histology. The wire myography data were used to derive compliance, stress-strain, and incremental elastic modulus curves. RESULTS: Vonapanitase treatment reduced @entity2362 ( @entity13403 ) content by 60% and decreased elastic fiber histologic staining. Vonapanitase-treated rings experienced less @entity2701 at any level of stretch and as a result had shifts in the compliance and stress-strain curves relative to vehicle-treated rings. Vonapanitase treatment did not alter the incremental elastic modulus curve. CONCLUSIONS: Vonapanitase treatment of @entity731 @entity1 peripheral arteries after ex vivo balloon angioplasty fragmented @entity2362 in elastic fibers, decreased @entity2701 in the rings at any level of stretch, and altered the compliance and stress-strain curves in a manner predicting @entity384 in vivo. Based on this result, local treatment of balloon angioplasty sites may increase blood vessel diameter and thereby improve the success of balloon angioplasty in @entity3657 .
