Functional analysis of the role of @entity2041 peroxidase (GPx) in the ROS signaling pathway, hyphal branching and the regulation of XXXX biosynthesis in Ganoderma lucidum.

@entity141 @entity5568 @entity64656 @entity2041
UNASSIGNED: Ganoderma lucidum, a hallmark of traditional Chinese medicine, has been widely used as a pharmacologically active compound. Although numerous research studies have focused on the pharmacological mechanism, fewer studies have explored the basic biological features of this species, restricting the further development and application of this important mushroom. Because of the ability of this mushroom to reduce and detoxify the compounds produced by various metabolic pathways, @entity2041 peroxidase (GPx) is one of the most important antioxidant enzymes with respect to ROS. Although studies in both animals and plants have suggested many important physiological functions of GPx, there are few systematic research studies concerning the role of this enzyme in fungi, particularly in large basidiomycetes. In the present study, we cloned the GPx gene and created GPx-silenced strains by the down-regulation of GPx gene expression using RNA interference. The results indicated an essential role for GPx in controlling the intracellular @entity5568 content, hyphal branching, antioxidant stress tolerance, cytosolic @entity141 content and @entity64656 biosynthesis. Further mechanistic investigation revealed that GPx is regulated by intracellular @entity5568 levels and suggested that crosstalk occurs between GPx and intracellular @entity5568 . Moreover, evidence was obtained indicating that GPx regulation of hyphal branching via ROS might occur independently of the cytosolic @entity141 content. Further mechanistic investigation also revealed that the effects of GPx on @entity64656 synthesis via ROS are regulated by the cytosolic @entity141 content. Taken together, these findings indicate that ROS have a complex influence on growth, development and secondary metabolism in fungi and that GPx serves an important function. The present study provides an excellent framework to identify GPx functions and highlights a role for this enzyme in ROS regulation.
