Analysis of heavy metal immunotoxicity by multiparameter flow cytometry: correlation of flow cytometry and immune function data in B6CF1 XXXX .

@entity13101 @entity558 @entity8990 @entity19 @entity10222 @entity3409 @entity56778 @entity6689 @entity3804 @entity3414
Bone marrow and spleen cells obtained from female B6C3F1 @entity19 given a single i.p. exposure to @entity56778 (0.9 mg/kg), @entity10222 (12 mg/kg), or @entity13101 (12 mg/kg), were studied using flow cytometry, immunologic, and hematologic assays. Significant changes were detected in subpopulations of bone marrow cells using multiparameter flow cytometry within 1 day following treatment with @entity3409 or lead. Bone marrow cells obtained from B6C3F1 @entity19 5 days after treatment with @entity3409 or lead were found to have a decreased number of cells expressing @entity3804 , 55-7.2, 14.8, and @entity6689 antigens, suggesting a shift to immature cell types. An increase in the number of progenitor cells (CFU-C) obtained from the bone marrow of @entity19 treated with heavy metals was also noted 5 days after exposure to @entity3409 or lead. A time-dependent suppression of the in vitro primary humoral immune response of spleen cells to SRBCs, TNP-Ficoll and TNP- @entity558 was produced by @entity3409 or lead treatment. Suppression of the mitogenic response of spleen cells to Con A, @entity8990 , and @entity558 was also found to be time-dependent. Spleen cell surface marker expression ( @entity3804 , @entity6689 , @entity3414 and 14.8) was altered in response to @entity3409 or lead treatments, but these changes did not appear to correlate with the humoral immunity or mitogen-induced proliferation data. These studies demonstrate that changes in cell surface markers on discrete subpopulations of lymphoid cells present in the spleens of heavy metal exposed @entity19 may not correlate with alterations in the functional activity of these cells. However, changes in @entity19 bone marrow surface markers in response to @entity3409 or lead treatment predicts a shift to immature cell types, which appeared to correlate with the increase in CFU-C activity.
