Selection of housekeeping genes for gene expression studies in a @entity35 model of @entity2600 -induced XXXX .

@entity26651 @entity2600 @entity64299 @entity64298 @entity1711 @entity33 @entity6175 @entity35 @entity11017
BACKGROUND/AIMS: @entity33 is the term used to describe damage caused by chemotherapy to mucous membranes of the alimentary tract. RT-PCR has recently been utilised to determine the molecular events that occur in @entity33 . As this method relies on the use of a validated endogenous control, this study aims to validate commonly used housekeeping genes in an @entity2600 -induced @entity33 model. METHODS: @entity35 were administered @entity2600 and sacrificed at different time points, in particular 1, 24, 72 and 144 h following treatment. Histopathological damage was assessed by @entity6175 and @entity1711 staining. RT-PCR was used to evaluate the expression of 11 housekeeping genes. Expression stability was determined by the Normfinder program. @entity11017 was used as a target gene to validate the appropriateness of the top-ranking housekeeping gene. RESULTS: For normalisation to multiple housekeeping genes, the most stable combination across all time points in the jejunum was @entity64298 / @entity26651 and in the colon @entity26651 /b-actin. @entity64299 and GAPDH were the most variable genes in the jejunum and colon where they were 4.4 and 3.2 fold upregulated following @entity2600 , respectively. CONCLUSIONS: For normalisation of @entity2600 -induced @entity33 gene expression studies, a combination of @entity64298 / @entity26651 and @entity26651 /b-actin should be used in the jejunum and colon, respectively. @entity26651 is the most favourable if restricted to a single housekeeping gene across all time points.
