Early diagnosis of XXXX by detection of dengue viral antigen in peripheral blood mononuclear cell.

@entity1 @entity778 @entity2822 @entity281 @entity7643 @entity779
BACKGROUND: An essential requirement related to treatment of @entity281 @entity1 is a rapid and accurate detection of dengue virus during febrile stage of the disease. OBJECTIVE: The study examined using direct immunofluorescence staining the presence of dengue viral antigen in peripheral blood mononuclear cells (PBMCs). METHODS: Four hundred forty-five blood samples from 164 @entity1 with @entity779 , @entity7643 , and other febrile illnesses were collected daily from the day of admission until discharge and also at convalescent stage. Blood smear was stained with @entity2822 -conjugated polyvalent dengue antiserum and examined under a fluorescent microscope. @entity281 was confirmed by virus isolation and/or dengue-specific IgM and IgG enzyme linked immunosorbent assay test. RESULTS: Dengue viral antigens were found in most PBMC samples of @entity281 @entity1 collected on the day before defervescence and continued until 2 to 3 days afterward. The number of dengue viral antigen positive PBMCs was highest in @entity1 with @entity7643 . Sensitivity and specificity of this method during the febrile stage was 93.8% (95% confidence interval: 88.8%-98.9%) and 100%, respectively. The positive and negative predictive values were 100% and 69.0%, respectively. CONCLUSION: Detection of dengue viral antigen in direct PBMC smear provides a useful and rapid technique for early diagnosis of @entity778 .
