Dominant expression of porcine XXXX in the uterus during a luteal phase.

@entity1 @entity1707 @entity172 @entity1420 @entity36931 @entity134 @entity460 @entity35 @entity4855
@entity36931 ( @entity4855 ) is a member of intracellular @entity460 binding proteins, which have a high affinity to @entity460 . @entity4855 is mainly expressed in the @entity1 intestine, uterus and placenta, and is regulated in tissue- and species-specific manners. Previous studies have shown that @entity4855 expression is mainly controlled by @entity134 hormones and their receptors. Thus, we further investigated the expression and regulation of @entity4855 during an estrus cycle in the @entity1707 uterus by Northern blot and immunoblot analysis in this study. In addition, serum levels of @entity172 (E2) and @entity1420 (P4) were measured using ELISA. The @entity4855 mRNA is highly expressed in the porcine uterus during a luteal phase compared to a follicular phase, and its mRNA level in a luteal phase is increased up to 10-fold compared to a follicular phase. In parallel to the level of @entity4855 mRNA, the @entity4855 protein is also dominantly expressed in the porcine uterus, and strongly expressed in the epithelium and glands of the porcine uterus during a luteal phase. Although, the localization of the @entity4855 protein is scarcely detected at follicular phase, it is dominantly expressed in the porcine uterus during a luteal phase. In addition, the serum P4 level was significantly increased during a luteal phase compared to a follicular phase, whereas no difference was observed in E2 levels between follicular and luteal phases, indicating that the ratio of P4/E2 is remarkably increased in porcine uterus during a luteal phase compared to a follicular phase. In conclusion, these results suggest that P4 may play an important role in the up-regulation of @entity4855 gene in the porcine uterus in a luteal phase, which is unlike the condition in the @entity35 uterus. In addition, the porcine @entity4855 may be dominantly expressed in the epithelium and glandular structure of @entity1707 uterus during a luteal phase. It may also be differentially regulated during this cycle presumably by @entity134 hormones, especially up-regulated P4 levels in this tissue.
