[Determination of XXXX in @entity1 plasma by microemulsion liquid chromatography].

@entity1 @entity3208 @entity5494 @entity32045 @entity6173 @entity1875 @entity10439 @entity1543
A microemulsion liquid chromatographic (MELC) method was developed for the determination of @entity1875 in @entity1 plasma. The assay was performed on a Hypersil BDS @entity6173 column, and the effects of each component in the microemulsion mobile phase on the elution were investigated. The optimized microemulsion mobile phase consisted of 0.5% @entity1543 containing 3.0% @entity3208 , 0.8% @entity32045 , 6.0% @entity10439 . The flow rate was 1.0 mL/min, and the separation was operated at ambient temperature. The fluorescence excitation and emission wavelengths were 274 nm and 312 nm, respectively. The plasma samples were diluted with the mobile phase and centrifuged before injection. The calibration curve of @entity1875 was linear within the range of 0.25 - 10 microg/mL. The method recoveries were (98.2 +/- 1.9)% - (104.6 +/- 2.2)%. The intra-day @entity5494 ( @entity5494 ) of peak area were 1.42% -2.43%, and the inter-day @entity5494 were 2.75% - 4.79%. The method is simple and reproducible. It can be used for the determination of @entity1875 in @entity1 plasma.
