Progression of @entity19 XXXX is associated with the orchestrated deregulation of miR-200 family members, @entity67897 and their common targets.

@entity146 @entity5 @entity1489 @entity67897 @entity19 @entity55867 @entity105 @entity24090
UNASSIGNED: MicroRNAs are small, non-coding RNAs which regulate post-transcriptionally hundreds of target mRNAs. Given that their expression is deregulated in several @entity5 types, they represent potential diagnostic, prognostic, and predictive biomarkers, as well as next-generation therapeutic targets. Nevertheless, the involvement of miRNAs in @entity1489 , a @entity5 type with increasing prevalence, is not extensively studied, and their comprehensive characterization as regard to the initiation, promotion, and progression stages is missing. To this end, we exploited a well-established multistage @entity19 @entity105 model in order to identify miRNAs consistently implicated in different stages of @entity105 . The cell lines comprising this model were subjected to miRNA expression profiling using microarrays, followed by bioinformatics analysis and validation with Q-PCR, as well as treatment with miRNA modulators. We showed that among all deregulated miRNAs in our system, only a functionally coherent group consisting of the miR-200 family members and @entity67897 -5p displays a pattern of progressive co-downregulation from the early toward the most @entity146 stages of @entity105 . Their overlapping, co-regulated putative targets are potentially inter-associated and, of these, the @entity55867 -related @entity24090 is overexpressed toward @entity146 stages. Ectopic expression of @entity67897 -5p in spindle @entity5 cells reduces @entity24090 , mitigates cell invasiveness, decreases proliferation, and delays @entity5 onset. We conclude that deregulation of this miRNA group is primarily associated with @entity146 phenotypes of @entity1489 cells. Restoration of the @entity67897 -5p member of this group in spindle cells reduces the expression of critical, co-regulated targets that favor @entity5 progression, thus reversing the @entity55867 characteristics.    2015 Wiley Periodicals, Inc.
