Measurement of XXXX -induced @entity1931 and @entity1286 -induced @entity1337 in @entity35 : dorsal vs plantar mechanical stimulation of the hindpaw.

@entity1931 @entity1337 @entity3795 @entity158 @entity1286 @entity35
OBJECTIVE: To compare the sensitivity of stimulating the plantar and dorsal hindpaw surfaces in the detection of mechanical @entity1931 and @entity1286 @entity1337 . BACKGROUND: Several approaches are used to assess nociceptive reactivity to mechanical stimulation in animal models of @entity158 . Although certain techniques seem to be favored for studying specific nociceptive conditions, the differences between techniques have not been directly compared and characterized. We chose to compare methods employing stimulation applied to the dorsum of the paw with stimulation of the plantar surface to demonstrate the utility of each approach in determining baseline nociceptive thresholds, changes in those thresholds after injury, and analgesic efficacy. METHODS: Withdrawal thresholds from mechanical stimulation applied to the dorsal and plantar surface of the hindpaw were measured in @entity35 treated with @entity1286 after receiving subcutaneous injections of complete Freund's adjuvant ( @entity3795 ) using Semmes-Weinstein (S-W) monofilaments and electro von Frey (EVF) stimulation. RESULTS: In contrast to stimulation of the dorsal surface, plantar hindpaw stimulation seldom elicited an aversive withdrawal response. Differences in withdrawal response from baseline were only detectable within the first 5 hours post- @entity3795 and only with EVF stimulation. No significant differences in stimulation techniques were observed after the initial 5-hour window. Effective dose 50 (ED(50)) for analgesic efficacy was consistently lower using dorsal stimulation. CONCLUSIONS: Stimulation of the plantar surface of the paw is superior for detecting small changes in paw sensitivity at very low stimulus intensities, whereas stimulation of the dorsal surface is superior for delineating baseline @entity158 thresholds and for detecting robust @entity1337 . CLINICAL RELEVANCE: Reliable and sensitive assessment of animal @entity158 behaviors is critical to translational @entity158 research. This study demonstrates the importance of using proper test protocols in animal studies and its implication in preclinical screening of potential analgesics.
