Protective Effects of Antiplacental Growth Factor Antibody Against Light-Induced XXXX in @entity19 .

@entity2000 @entity19 @entity13785 @entity36605 @entity989 @entity528
Purpose: @entity36605 ( @entity36605 ) is part of the VEGF family and is known to be involved in angiogenesis, vasopermeability, and neuroprotection. Recently, @entity36605 has been reported as a novel therapeutic target for wet @entity13785 . However, there are few reports about the effect of @entity36605 against dry @entity13785 . Previously, we reported that @entity36605 has protective effects against retinal neuronal cell damage in vitro. Therefore, we investigated the effects of @entity36605 against @entity528 . Methods: In this study, @entity19 were exposed to white light at 8000 lx for 3 hours to induce @entity989 , which was evaluated by recording the electroretinogram amplitude and measuring the outer nuclear layer (ONL) thickness. The @entity19 were injected intravitreally with @entity36605 before light exposure, @entity36605 after light exposure, or anti- @entity36605 antibody before light exposure. RPE-choroid-sclera flat mounts were immunostained with anti-ZO-1 antibody to evaluate the disruption of retinal pigmented epithelium (RPE) cell-cell junctional integrity after light exposure. Furthermore, the expression of VEGF receptor in the retina and RPE-choroid complex after light exposure was measured using Western blot analysis. Results: Contrary to the expected outcome, @entity36605 treatment exacerbated the light-induced @entity989 and ONL thinning. In contrast, anti- @entity36605 treatment significantly improved the light-induced @entity2000 . The disruption of RPE cell-cell junctional integrity after light exposure was suppressed by anti- @entity36605 treatment. Moreover, the VEGF receptor, which is involved in blood-retinal barrier breakdown, was up-regulated after light exposure. Conclusions: These findings suggest that anti- @entity36605 antibody has protective effects against light-induced @entity2000 in the @entity19 retina through inhibition of RPE breakdown after light exposure. Thus, anti- @entity36605 antibody may be useful therapeutic agents in dry @entity13785 .
