Spinal NF-kB and Chemokine Ligand 5 Expression during Spinal Glial Cell Activation in a XXXX Model.

@entity17037 @entity5737 @entity1931 @entity1214 @entity13373 @entity128 @entity158 @entity14568 @entity8638 @entity9
BACKGROUND: The NF-kB pathway and @entity5737 ( @entity5737 ) are involved in @entity158 modulation; however, the precise mechanisms of their interactions in chronic @entity9 have yet to be established. METHODS: The present study examined the roles of spinal NF-kB and @entity5737 in a @entity9 model after @entity8638 ( @entity8638 ) surgery. @entity8638 -induced @entity158 facilitation was evaluated using the Plantar and von Frey tests. The changes in NF-kB and @entity5737 expression were analyzed by immunohistochemistry and Western blot analyses. RESULTS: Spinal NF-kB and @entity5737 expression increased after @entity8638 surgery. Repeated intrathecal infusions of @entity13373 ( @entity14568 , a NF-kB inhibitor) decreased @entity5737 expression, inhibited the activation of microglia and astrocytes, and attenuated @entity8638 -induced @entity1931 and @entity1931 . Intrathecal injection of a @entity5737 -neutralizing antibody attenuated @entity8638 -induced @entity158 facilitation and also suppressed spinal glial cell activation after @entity8638 surgery. However, the @entity5737 -neutralizing antibody did not affect NF-kB expression. Furthermore, selective glial inhibitors, @entity1214 and @entity17037 , attenuated the @entity1931 induced by intrathecal @entity5737 . CONCLUSIONS: The inhibition of spinal @entity5737 expression may provide a new method to prevent and treat @entity128 -induced @entity9 .
