Regulated expression of collagenases XXXX and stromelysins @entity12792 by @entity1 corneal epithelial cells.

@entity1 @entity12792 @entity72896 @entity3334 @entity1356 @entity310 @entity3403 @entity1193 @entity72894 @entity2843 @entity72893 @entity72895
PURPOSE: This study investigated the regulated expression of collagenases ( @entity72893 ) and stromelysins ( @entity12792 ) by @entity1 corneal epithelial cells treated with @entity1356 , @entity310 , and @entity3403 , a medication used to treat ocular surface diseases. METHODS: Primary @entity1 corneal epithelial cell cultures were treated with @entity1356 or @entity310 , with or without their corresponding inhibitors. Total RNA extracted from cells treated for 4 to 24 hours was subjected to semiquantitative RT-PCR and Northern hybridization. Conditioned media from 24-hour-treated cultures were evaluated for MMP production by ELISA and activity assays. RESULTS: Semiquantitative RT-PCR and Northern hybridization revealed that the mRNAs of @entity3334 were dose dependently upregulated by @entity1356 and @entity310 , whereas @entity72894 and @entity2843 were not altered, in corneal epithelial cells. MMP ELISA and activity assays confirmed this dose-dependent increase in @entity3334 protein production in conditioned media by @entity1356 and @entity310 . This stimulated production was inhibited by their neutralizing antibodies and by @entity1193 . @entity3403 suppressed stimulated @entity72895 production at both the mRNA and protein levels. CONCLUSIONS: This study demonstrated that @entity1356 and @entity310 upregulate collagenases ( @entity3334 ) and stromelysins ( @entity12792 ) in @entity1 corneal epithelial cells. @entity3403 suppresses stimulated @entity72896 at the mRNA and protein levels, which suggests that collagenases and stromelysins may play a role in the pathogenesis of sterile corneal ulceration and other ocular surface diseases.
