Cloning and expression of a cDNA coding for a XXXX monocyte-derived plasminogen activator inhibitor.

@entity1 @entity1522 @entity1472 @entity2289 @entity535 @entity377 @entity10663 @entity10933 @entity563 @entity526
@entity1 monocyte-derived plasminogen activator inhibitor ( @entity10663 ) was purified to homogeneity from the U937 cell line and partially sequenced. Oligonucleotide probes derived from this sequence were used to screen a cDNA library prepared from U937 cells. One positive clone was sequenced and contained most of the coding sequence as well as a long incomplete 3' untranslated region (1112 base pairs). This cDNA sequence was shown to encode @entity10663 by hybrid-select translation. A cDNA clone encoding the remainder of the @entity10663 mRNA was obtained by primer extension of U937 poly(A)+ RNA using a probe complementary to the @entity10663 coding region. The coding sequence for @entity10663 was placed under the control of the lambda PL promoter, and the protein expressed in @entity526 formed a complex with urokinase that could be detected immunologically. By @entity377 sequence analysis, @entity10663 cDNA encodes a protein containing 415 @entity1522 with a predicted unglycosylated Mr of 46,543. The predicted amino acid sequence of @entity10663 is very similar to placental @entity10933 (3 @entity535 differences) and shows extensive homology with members of the @entity563 protease inhibitor (serpin) superfamily. @entity10663 was found to be more homologous to ovalbumin (37%) than the endothelial plasminogen activator inhibitor, @entity2289 (26%). Like ovalbumin, @entity10663 appears to have no typical @entity1472 -terminal signal sequence. The 3' untranslated region of the @entity10663 cDNA contains a putative regulatory sequence that has been associated with the inflammatory mediators.
