Quantitative proteomic analysis of XXXX cells infected with Dengue virus by label-free LC-MS.

@entity1 @entity1792 @entity5959 @entity281 @entity2388
Dengue is an important and growing public health problem worldwide with an estimated 100million new clinical cases annually. Currently, no licensed drug or vaccine is available. During @entity281 in @entity1 , liver cells constitute one of the main targets of dengue virus (DENV) replication. However, a clear understanding of dengue pathogenesis remains elusive. In order to gain a better reading of the cross talk between virus and host cell proteins, we used a proteomics approach to analyze the host response to DENV infection in a hepatic cell line @entity5959 . Differences in proteome expression were assayed 24h post-infection using label-free LC-MS. Quantitative analysis revealed 155 differentially expressed proteins, 64 of which were up-regulated and 91 down-regulated. These results reveal an important decrease in the expression of enzymes involved in the glycolytic pathway, @entity1792 cycle, and @entity2388 metabolism. This study provides large-scale quantitative information regarding protein expression in the early stages of infection that should be useful for better compression of the pathogenesis of dengue. BIOLOGICAL SIGNIFICANCE: @entity281 involves alterations in the homeostasis of the host cell. Defining the interactions between virus and cell proteins should provide a better understanding of how viruses propagate and cause disease. Here, we present for the first time the proteomic analysis of hepatocytes ( @entity5959 cells) infected with DENV-2 by label-free LC-MS. This article is part of a Special Issue entitled: Proteomics, mass spectrometry and peptidomics, Cancun 2013.
