Millettia pachycarpa exhibits anti-inflammatory activity through the suppression of LPS-induced NO/ XXXX expression.

@entity32 @entity137 @entity70817 @entity4 @entity3542 @entity70816 @entity3088 @entity13633 @entity3352 @entity1068
The present study was designed to investigate the in vitro and in vivo anti-inflammatory activity of @entity3088 isolated from Millettia pachycarpa Benth. The seeds of M. pachycarpa Benth were extracted with @entity1068 and subjected to chromatographic separation for the isolation of bioactive compounds. Their structures were elucidated by spectroscopic methods. The anti-inflammatory activity of the compounds was investigated by evaluating the inhibition ability of NO production, @entity13633 activity and @entity13633 protein expression induced by LPS-stimulated RAW264.7 macrophages in vitro and the carrageenan-induced hind paw @entity4 model in vivo. Molecular docking simulation was also employed to obtain the binding parameters in the binding pocket of @entity13633 . Thirteen compounds (1-13) were isolated from Chinese herbal medicine M. pachycarpa Benth. Among them, @entity70816 (6) and @entity70817 (7) exhibited remarkable inhibitory rates of 66.5% and 57.7%, respectively, compared with that of 52.5% of @entity3352 in LPS-induced macrophages cells. @entity70816 (6) with low @entity137 (IC50 > 100  m) exhibited better inhibitory effects to positive control of 1400W on @entity13633 activity at the concentration of 10  m. Western blot assay revealed that @entity70816 (6) inhibited @entity13633 protein expression in RAW264.7 cells and molecular docking simulation showed that @entity70816 (6) fit well into the binding pocket of @entity13633 . In the carrageenan-induced paw @entity4 model, our data revealed that the anti-inflammatory potential of @entity70816 (6) at 10 mg/kg showed comparable inhibitory ability to @entity3352 at 5 h while a higher concentration of @entity70816 (6) at 50 mg/kg showed higher inhibitory activity than @entity3352 , which was further confirmed by plasma levels of @entity3542 . The overall results suggest that @entity70816 (6) might be used as a potential therapeutic agent for @entity32 .
