Multilocus sequence typing of XXXX isolates from Burn Intensive Care Unit (BICU) in Iran.

@entity1 @entity281 @entity5132 @entity580
UNASSIGNED: Burn intensive care unit @entity1 are specifically exposed to @entity580 caused by @entity5132 . Superficial carriage of @entity5132 is a potential source of @entity281 and dissemination, and typing methods could be useful to trace the different isolates. Multilocus sequence typing is a powerful genotyping method for pathogenic microorganisms, including @entity5132 . Thirty clinical isolates of @entity5132 obtained from 22 @entity1 that admitted to the Burn Intensive Care Unit (BICU) from a burn hospital at Sari, Mazandaran state, Iran were epidemiologically studied by multilocus sequence typing (MLST). Seventy five variable nucleotide sites were found. Sixty two alleles were identified among the seven loci of the @entity5132 isolates and one new allele was obtained. Eighteen Diploid Sequence Types (DSTs) were identified and among those 10 were new. These isolates belonged to nine clonal clusters (CCs) while two isolates occurred as singletons. Eleven (36.7%) isolates belonged to CC 124 after eBURST analysis and 13 isolates (43.3%) were assigned to clade 4. Approximately 17% of the 30 isolates belonged to clade 1 (CC 69 and CC766). Isolates from several @entity1 with burns were found to be genetically related. Some @entity1 yielded multiple isolates with identical DSTs suggest colonization or @entity281 caused by cross contamination between @entity1 . Therefore, isolates that show identical or similar allelic pro   les are presumed to be identical or closely related and may use to evaluate the genetic relationships between isolates from a speci   c environment such as BICU.
