Establishment and characterization of an immortalized XXXX hepatic stellate cell line.

@entity10740 @entity82 @entity101 @entity5 @entity19 @entity842 @entity1827 @entity1810 @entity35 @entity672
Hepatic stellate cells (HSCs) play an important role in liver @entity82 and @entity101 . This study established a new @entity35 HSC cell line LSC-1. Liver ex vivo perfusion with collagenase IV and density gradient centrifugation were used to isolate @entity35 HSC. Cells have been maintained in culture for multiple passages. LSC-1 cell biological characteristics were studied. LSC-1 cell have been maintained in culture over 100 passages. This new HSC cell line express telomerase reverse transcriptase (TRT) and @entity10740 , suggesting that it is immortalized spontaneously. LSC-1 cells have a doubling time of 46 hours and their growth is serum-dependent. Karyotypic analysis revealed that LSC-1 cells possess normal chromosome phenotype. Moreover, LSC-1 cells do not grow in soft agar or induce @entity5 in @entity19 , suggesting that they are not transformed. LSC-1 cells express desmin, glial fibrillary acidic proteins (GFAP), collagen type I and III, a-smooth muscle actin (a-SMA), @entity1810 ( @entity1810 ), @entity672 ( @entity672 ) and inducible @entity842 synthase (iNOS). @entity1810 stimulation increased collagen type I and III expression in LSC-1 cells. Additionally, LSC-1 cells proliferate in response to PDGF-BB, and contract in response to @entity1827 (ET-1). In summary, LSC-1 cells exhibit activated HSC phenotype characteristics, and therefore are useful tool to study the pathogenesis of liver @entity82 and @entity101 .
