Growth, viral production and metabolism of a XXXX zea cell line in serum-free culture.

@entity1160 @entity3265 @entity13708 @entity2734 @entity880 @entity258 @entity413
Insect cell cultures have been extensively utilised for means of production for heterologous proteins and biopesticides. Spodoptera frugiperda (Sf9) and Trichoplusia ni (High Fivetrade mark) cell lines have been widely used for the production of recombinant proteins, thus metabolism of these cell lines have been investigated thoroughly over recent years. The @entity13708 zea cell line has potential use for the production of a biopesticide, specifically the @entity13708 single-nucleocapsid nucleopolyhedrovirus (HaSNPV). The growth, virus production, nutrient consumption and waste production of this cell line was investigated under serum-free culture conditions, using SF900II and a @entity258 ( @entity258 ). The cell growth (growth rates and population doubling time) was comparable in SF900II and @entity258 , however, lower biomass and cell specific virus yields were obtained in @entity258 . H. zea cells showed a preference for @entity2734 over @entity1160 , similar to the High Fivetrade mark cells. @entity3265 was accumulated to significantly high levels (16 mM) in SF900II, which is an @entity2734 and @entity1160 rich medium. However, given the absence of @entity2734 and @entity1160 in the medium ( @entity258 ), H. zea cells adapted and grew well in the absence of these substrates and no accumulation of @entity3265 was observed. The adverse effect of @entity3265 on H. zea cells is unknown since good production of biologically active HaSNPV was achieved in the presence of high @entity3265 levels. H. zea cells showed a preference for maltose even given an abundance supply of free @entity413 . Accumulation of @entity880 was observed in H. zea cell cultures.
