The Expression of Markers Related to Ovarian Germline Stem Cells in the XXXX Ovarian Surface Epithelium and the Correlation with Notch Signaling Pathway.

@entity10351 @entity19 @entity5234 @entity68657 @entity2670 @entity7002 @entity29921 @entity18120
BACKGROUND/AIMS: Ovarian germline stem cells (OGSCs) have been shown to mainly exist in the @entity2670 ( @entity2670 ), but the activity changes of germline stem cells during different reproductive stages and the potential regulatory signaling pathway are still unknown. The Notch signaling pathway plays a key role in cell development, primordial follicles and stem cell proliferation. However, whether it plays a role in the proliferation of OGSCs is unknown. Here, we analyzed the activity changes of germline stem cells and the correlation between germline stem cells and the Notch signaling pathway. METHODS: The expression of germline stem cell markers @entity29921 , Ooc4 and the Notch molecules @entity5234 , @entity10351 , and @entity18120 were detected during 3 days (3d), and 2, 12, 20 months (2m, 12m, 20m) @entity19 @entity2670 samples. @entity68657 , a specific inhibitor of the Notch pathway, was used to observe the influence of Notch signaling in the germline stem cells. RESULTS: The results showed that the levels of @entity29921 and @entity7002 decreased substantially with reproductive age in @entity2670 , and the same tendency was detected in the Notch signaling molecules @entity5234 , @entity10351 and @entity18120 . Dual-IF results showed that the germline stem cell markers were co-expressed with Notch molecules in the @entity2670 . While, the expression of @entity29921 and @entity7002 were reduced when the ovaries were treated with @entity68657 and the levels were attenuated with increasing dose of @entity68657 . CONCLUSION: Taken together, our results indicate that the viability of OGSCs decreased with the age of the @entity19 ovaries, and the activity of OGSCs in the @entity2670 may be related to the Notch signaling pathway.
