Orientation of gingival fibroblasts in simulated periodontal spaces in vitro. XXXX observations.

@entity1 @entity11968 @entity291 @entity1098 @entity4653
The present study examined the orientation of gingival fibroblasts in simulated periodontal spaces in vitro. Extracted @entity1 teeth were root planed followed by root resection and root canal instrumentation. The middle and cervical thirds of each root were cut transversely to create 600-micron thick sections. Cortical @entity291 bone was cut, sectioned and contoured to create bone rings 600 micron thick with an internal diameter large enough to accommodate a root slice leaving a circumferential space varying from approximately 0.1 to 1.0 mm. Root slices and bone rings were incubated in a solution of collagenase and hyaluronidase to remove all remaining soft tissue and partially demineralized in @entity1098 (18%) for 30 minutes. @entity1 gingival fibroblasts ( @entity11968 ) were plated to confluency in tissue culture dishes. The dentin slices were then gently placed over the @entity11968 monolayer along with bone rings around them to create simulated periodontal spaces. Control root slices were placed without bone rings around them. Cultures were maintained under standard tissue culture conditions. Representative specimens were obtained after 2, 3 and 4 weeks of culture and processed for @entity4653 ( @entity4653 ). At 2 weeks, the @entity11968 had formed sheets of cells attached to the periphery of the root slices at one end and to the inner surface of bone rings at the other end. The orientation of cell sheets varied from being perpendicular to the periphery of the slice to oblique. At 3 and 4 weeks, the density and size of cell sheets increased and the orientation was maintained.(ABSTRACT TRUNCATED AT 250 WORDS)
