Transduction and oncolytic profile of a potent replication-competent adenovirus 11p vector (RCAd11pGFP) in XXXX cells.

@entity137 @entity11650 @entity5 @entity19 @entity189 @entity806 @entity694
Replication-competent adenovirus type 5 ( @entity11650 ) vectors promise to be more efficient gene delivery vehicles than their replication-deficient counterparts, and chimeric @entity11650 vectors that are capable of targeting @entity806 are more effective than @entity11650 vectors with native fibers. Although several strategies have been used to improve gene transduction and oncolysis, either by modifying their tropism or enhancing their replication capacity, some @entity5 cells are still relatively refractory to infection by chimeric @entity11650 . The oncolytic effects of the vectors are apparent in certain @entity5 but not in others. Here, we report the biological and oncolytic profiles of a replication-competent adenovirus 11p vector (RCAd11pGFP) in @entity694 cells. @entity806 was abundantly expressed in all cells studied; however, the transduction efficiency of RCAd11pGFP varied. RCAd11pGFP efficiently transduced HT-29, HCT-8, and LS174T cells, but it transduced T84 cells, derived from a @entity694 metastasis in the lung, less efficiently. Interestingly, RCAd11p replicated more rapidly in the T84 cells than in HCT-8 and LS174T cells and as rapidly as in HT-29 cells. Cell @entity137 and proliferation assays indicated that RCAd11pGFP had the highest cell-killing activities in HT29 and T84 cells, the latter of which also expressed the highest levels of glycoproteins of the @entity189 embryonic antigen (CEA) family. In vivo experiments showed significant growth inhibition of T84 and HT-29 @entity5 in xenograft @entity19 treated with either RCAd11pGFP or Ad11pwt compared to untreated controls. Thus, RCAd11pGFP has a potent cytotoxic effect on @entity694 cells.
