The Inhibitory Effect of XXXX on Melanoma Cancer @entity3 and Invasiveness In vitro and In vivo.

@entity1 @entity19 @entity5780 @entity1311 @entity15174 @entity3 @entity204 @entity320
UNASSIGNED: @entity320 cell @entity3 is the primary cause of @entity1 @entity204 . Thus, various treatment strategies have been developed to prevent @entity3 . @entity15174 (AA) is an organic compound commonly found in trees. This study is aimed to investigate the antimetastatic activity of AA in B16F10-xenografted C57BL/6 @entity19 and assess the anticancer activity of AA in combination with @entity1311 in @entity320 cells. AA effectively reduced the formation of lung metastases by approximately 92.8%. AA treatment inhibited migratory potential ([Formula: see text]), invasion ([Formula: see text]), and motility ([Formula: see text]) of highly metastatic @entity19 @entity320 cells in vitro. Zymography revealed that AA reduced the proteinase activities of @entity5780 and urokinase-type plasminogen activator. Molecular analyses showed that AA reduced Akt phosphorylation and activating protein-1 DNA-binding activity by Western blot and electrophoretic mobility shift assay (EMSA), respectively. In summary, AA effectively inhibited B16F10 lung @entity3 , and 50[Formula: see text][Formula: see text]M AA did not affect the viability of @entity19 cells. AA improved the efficacy of @entity1311 and demonstrated strong anticancer activity on @entity320 cells. These results suggested that AA could be used as an antimetastatic agent or as an adjuvant for anticancer therapy.
