Modulation of glucocorticoid hormone receptor levels in XXXX lymphoid tissue following treatment with @entity3800 in vivo.

@entity2995 @entity586 @entity1573 @entity71294 @entity342 @entity3800
Lymphatic tissues are highly sensitive to @entity3800 and @entity3800 are thought to contribute to sex differences in the immune response. In this study we have examined the effects of @entity3800 on cytosolic glucocorticoid receptor levels in lymphoid tissues. The immature @entity1573 was chosen for our experimental model because it allows the separate evaluation of the bursa of Fabricius (primarily B-cells) compared to the thymus (primary T-cells). Treatment with @entity586 (a potent @entity342 in chicks) for 3-12 days in vivo reduced the cytosolic glucocorticoid ( @entity2995 -[3H]) receptors in bursa tissue to approximately 42% of control levels after 5 days and less than or equal to 5% of control levels after 7 days of treatment. The @entity1573 thymus tissues were still approximately 92% of control @entity2995 receptor levels after 5 days of @entity342 treatments. However the thymus levels had dropped to less than or equal to 5% of control values after 12 treatment days. Thus a difference in the rate of decrease in the bursa of Fabricius compared to the thymus was indicated. The blastogenesis index (BI), a measurement of the percentage of cells progressing through the cell cycle, was figured using fluorescent DNA staining with diamidino @entity71294 followed by flow cytometry analysis. After 3, 5, or 7 days of @entity342 treatment, the bursa of Fabricius from @entity586 treated chicks (2 mg/day/ @entity1573 ) had a mean BI = 11.17 (+/- 3.07 SD) which was significantly lower than the bursa of Fabricius from control chicks which showed a mean BI = 27.33 (+/- 3.42 SD). The thymus from @entity586 treated chicks had a mean BI = 19.57 (+/- 2.19 SD) which was slightly but not significantly higher than the control thymus CI = 17.38 (+/- 0.89 SD). In summary, @entity342 treatment in vivo induced a decrease in the cytosolic glucocorticoid hormone receptor levels in both the @entity1573 thymus and bursa of Fabricius tissues while decreasing the blastogenesis index in the bursa cells but not in the thymus cells.
