Maitake (D fraction) mushroom extract induces apoptosis in XXXX cells by @entity2428 gene activation.

@entity1 @entity2428 @entity0 @entity15781 @entity5 @entity4674 @entity9342 @entity2383
For many years mushrooms have been used empirically in traditional medicine to treat several diseases. Study of the maitake mushroom, with its immunomodulatory and antitumoral properties, has led to the isolation of several bioactive compounds. One of these, D fraction, is known to reduce @entity5 cell viability. This study examined the effect of isolated D fraction on viability and apoptosis of @entity1 @entity0 cells (MCF7). These cells were treated with maitake (D fraction) extract at 18    g/mL, 36    g/mL, 91    g/mL, 183    g/mL, or 367    g/mL or were left untreated (control) for 24 hours. MCF7 incubation with the maitake extract resulted in decreased cell viability [ @entity15781 assay] in a dose-dependent manner. Apoptosis was statistically significantly increased in a dose-dependent manner at every concentration tested (terminal deoxynucleotidyl transferase-mediated @entity9342 nick-end labeling assay). Upon incubation with D fraction, a microarray assay revealed upregulation of @entity2428 and @entity4674 transcripts, 2 proteins directly involved in the apoptotic pathway. Reverse transcriptase polymerase chain reaction studies confirmed these findings; @entity2428 was one of most overexpressed gene, as observed by microarray assay. These findings confirm the apoptotic effect of maitake D fraction in @entity0 cells and further highlight the involvement of @entity4674 release to the cytoplasm. Cytoplasmic release of @entity4674 , another player in the apoptotic pathway, was also increased after incubation with D fraction in a dose-dependent manner. This finding indicates that the effect of this compound involves @entity2383 . The identification of the molecular mechanisms by which D fraction exerts its effects is crucial for the development of preventive and therapeutic strategies for @entity5 .
