XXXX receptor expression on @entity19 and @entity1 adipose tissue macrophages.

@entity1501 @entity1 @entity28 @entity14452 @entity19 @entity3913 @entity9536 @entity2935
OBJECTIVE AND DESIGN: Adipose tissue macrophages (ATMs) have been implicated in a number of @entity28 . Because the activated macrophages associated with many types of autoimmune and inflammatory diseases express a @entity1501 receptor (FR) that can be exploited for FR-targeted drug delivery, we examined the visceral adipose tissue of @entity28 @entity19 and @entity1 to determine whether ATMs also express FR that are accessible by @entity1501 conjugates. MATERIAL OR SUBJECTS: C57BL/6 or FATSO @entity19 fed on either a low- or high-fat diet were used in @entity19 studies. @entity1 adipose tissue were obtained from healthy volunteers during adipose reduction surgery. METHODS: Visceral adipose tissue was collected from both @entity28 @entity19 and @entity1 , collagenase digested, and stained with @entity1501 -Oregon Green and antibodies for macrophage markers including F4/80, @entity3913 receptor ( @entity14452 ), @entity9536 , and @entity2935 . Cells were then examined for expression of the above markers by flow cytometry. Furthermore, the ability of @entity1501 conjugates to target the FR-expressing ATMs in @entity28 @entity19 was evaluated in vivo. RESULTS: A subset of the ATMs harvested from @entity28 @entity19 were found to express FR. Subpopulations of ATMs also simultaneously express both pro- and anti-inflammatory markers, and FR is expressed on both subsets. We then demonstrate that FR-expressing ATMs can be targeted with @entity1501 -linked fluorescent dyes in vivo. CONCLUSIONS: FR are expressed on multiple subsets of ATMs and these subsets can be targeted with @entity1501 -linked drugs, allowing for the possible development of FR-targeted therapies for @entity28 -related inflammatory diseases.
