Transforming growth factors beta1, @entity2263 and @entity1093 and their receptors are differentially expressed in XXXX peritoneal fibroblasts in response to @entity519 .

@entity1 @entity1093 @entity519 @entity2533 @entity1934 @entity6474 @entity2263 @entity298
PROBLEM: Little is known about the role of peritoneal fibroblasts in adhesion formation. This study determines the effect of @entity519 and @entity2533 treatment on the expression of @entity2533 -3 and TGF-betaI and betaII receptors in @entity1 peritoneal fibroblasts (HPF). TGF-beta isoforms and their receptors have been implicated as mediators of the healing process and adhesion development. METHODS: HPF were cultured under normal and @entity519 , and treated with and without (1 ng/mL) @entity2533 for 24 hr. Total RNA from each group was subjected to multiplex reverse transcriptase-polymerase chain reaction (RT/PCR) to quantitate @entity2533 -3 and TGF-betaI and betaII receptors messenger RNA (mRNA) levels. RESULTS: @entity519 resulted in a significant increase in @entity2533 (26%; P < 0.05), TGF-betaIR (34%; P < 0.05) and TGF-betaIIR (29%; P < 0.05) mRNA levels, with no effect on @entity6474 or @entity1093 . @entity2533 treatment resulted in a significant increase in @entity2533 (35%; P < 0.05), but a decrease in @entity6474 (22%; P < 0.05) and no effect on @entity1934 , TGF-betaIR or TGF-betaIIR. Combined treatment of @entity519 and @entity2533 caused a significant increase in @entity2533 (37%; P < 0.05), @entity6474 (12%; P < 0.05), TGF-betaIR (32%; P < 0.05) and TGF-betaIIR (34%; P < 0.05). There is no significant change in the mRNA levels of @entity1934 in any of the treatments. CONCLUSION: @entity519 and @entity2533 treatments of cultured HPF modulate the expression of @entity2533 , @entity2263 and @entity1093 increasing the ratio of @entity2533 and @entity2263 to @entity1093 , thus favoring the development of @entity298 .
