Mining and identification of XXXX synthesis genes active during camelina seed development using 454 pyrosequencing.

@entity5204 @entity5031 @entity844
BACKGROUND: Camelina (Camelina sativa L.) is well known for its high @entity5031 content and great resistance to environmental stress. However, little is known about the molecular mechanisms of @entity5031 biosynthesis in this annual oilseed crop. To gain greater insight into this mechanism, the transcriptome profiles of seeds at different developmental stages were analyzed by 454 pyrosequencing. RESULTS: Sequencing of two normalized 454 libraries produced 831,632 clean reads. A total of 32,759 unigenes with an average length of 642  bp were obtained by de novo assembly, and 12,476 up-regulated and 12,390 down-regulated unigenes were identified in the 20 DAF (days after flowering) library compared with the 10 DAF library. Functional annotations showed that 220 genes annotated as @entity844 biosynthesis genes were up-regulated in 20 DAF sample. Among them, 47 candidate unigenes were characterized as responsible for @entity5204 synthesis. To verify unigene expression levels calculated from the transcriptome analysis results, quantitative real-time PCR was performed on 11 randomly selected genes from the 220 up-regulated genes; 10 showed consistency between qRT-PCR and 454 pyrosequencing results. CONCLUSIONS: Investigation of gene expression levels revealed 32,759 genes involved in seed development, many of which showed significant changes in the 20 DAF sample compared with the 10 DAF sample. Our 454 pyrosequencing data for the camelina transcriptome provide an insight into the molecular mechanisms and regulatory pathways of @entity5204 biosynthesis in camelina. The genes characterized in our research will provide candidate genes for the genetic modification of crops.
