Biotechnology applications of XXXX in protein purification and formulations.

@entity969 @entity1522 @entity369 @entity653
@entity1522 are widely used in biotechnology applications. Since @entity1522 are natural compounds, they can be safely used in pharmaceutical applications, e.g., as a solvent additive for protein purification and as an excipient for protein formulations. At high concentrations, certain @entity1522 are found to raise intra-cellular osmotic pressure and adjust to the high salt concentrations of the surrounding medium. They are called "compatible solutes", since they do not affect macromolecular function. Not only are they needed to increase the osmotic pressure, they are known to increase the stability of the proteins. @entity969 , @entity653 and certain @entity1522 were used to enhance the stability of unstable proteins after isolation from natural environments. The mechanism of the action of these protein-stabilizing @entity1522 is relatively well understood. On the contrary, @entity369 was accidentally discovered as a useful reagent for assisting in the refolding of recombinant proteins. This effect of @entity369 was ascribed to its ability to suppress aggregation of the proteins during refolding, thereby increasing refolding efficiency. By the same mechanism, @entity369 now finds much wider applications than previously anticipated in the research and development of proteins, in particular in pharmaceutical applications. For example, @entity369 solubilizes proteins from loose inclusion bodies, resulting in efficient production of active proteins. @entity369 suppresses protein-protein interactions in solution and also non-specific adsorption to gel permeation chromatography columns. @entity369 facilitates elution of bound proteins from various column resins, including Protein-A or dye affinity columns and hydrophobic interaction columns. This review covers various biotechnology applications of @entity1522 , in particular @entity369 .
