@entity2533 promotes the migration and invasion of sphere-forming stem-like cell subpopulations in XXXX .

@entity5 @entity4730 @entity4731 @entity2533 @entity418 @entity3 @entity22346 @entity14215 @entity14959
UNASSIGNED: @entity418 is one of the most lethal solid @entity5 . Mounting evidence demonstrates that @entity5 stem cells (CSCs) are able to cause @entity5 initiation, @entity3 and responsible for chemotherapy and radiotherapy failures. As CSCs are thought to be the main reason of therapeutic failure, these cells must be effectively targeted to elicit long-lasting therapeutic responses. We aimed to enrich and identify the @entity418 cell subpopulation with stem-like properties and help to develop new target therapy strategies for CSCs. Here, we found @entity418 cells KYSE70 and TE1 could form spheres in ultra low attachment surface culture and be serially passaged. Sphere-forming cells could redifferentiate and acquire morphology comparable to parental cells, when return to adherent culture. The sphere-forming cells possessed the key criteria that define CSCs: persistent self-renewal, overexpression of stemness genes ( @entity4731 , @entity14215 and @entity4730 ), reduced expression of differentiation marker @entity14959 , chemoresistance, strong invasion and enhanced tumorigenic potential. @entity22346 , @entity2533 ( @entity2533 ) inhibitor, significantly inhibited migration and invasion of sphere-forming stem-like cells and had no effect on sphere-forming ability. In conclusion, @entity418 sphere-forming cells from KYSE70 and TE1 cultured in ultra low attachment surface @entity5 stem cell properties, providing a model for CSCs targeted therapy. @entity2533 promotes the migration and invasion of sphere-forming stem-like cells, which may guide future studies on therapeutic strategies targeting these cells.
