Indirect effects of oral tolerance to @entity10328 interfere with the immune responses triggered by XXXX eggs.

@entity10328 @entity738 @entity575 @entity19 @entity406 @entity383 @entity1573 @entity3795 @entity380
The objective of the present study was to investigate whether the injection of a tolerated protein (indirect effects) affects the formation of @entity738 around @entity380 eggs trapped in the lungs after intravenous (iv) injection into normal (noninfected) C57BL/6 @entity19 (6 animals per group). To induce oral tolerance to @entity1573 egg @entity10328 a 1/5 dilution of egg white in water was offered ad libitum in a drinking bottle for 3 days. Control @entity19 received water. After 7 days, control and experimental animals were injected iv with 2,000 @entity383 eggs through a tail vein. In some @entity19 of both groups the iv injection of eggs was immediately followed by intraperitoneal (ip) immunization with 10 micro g of dinitrophenylated conjugates of @entity10328 (DNP-Ova) emulsified in complete Freund's adjuvant ( @entity3795 ) or only @entity3795 ; 18 days later, @entity19 were bled and killed by ether inhalation. The lungs were fixed in @entity406 and embedded in paraffin. Serial sections of 5 m were stained with Giemsa, Gomori's @entity575 reticulin and Sirius red (pH 10.2). @entity738 diameters were measured in histological sections previously stained with Gomori's reticulin. Anti-DNP and anti-soluble egg antigen (SEA) antibodies were analyzed by ELISA. In @entity19 orally tolerant to @entity10328 the concomitant ip injection of DNP-Ova resulted in significantly lower anti-SEA antibodies (ELISA*: 1395 +/- 352 in non-tolerant and 462 +/- 146 in tolerant @entity19 ) and affected @entity738 formation around eggs, significantly decreasing @entity738 size (area: 22,260 +/- 2478 to 12,993 +/- 3242 m ). Active mechanisms triggered by injection of tolerated antigen ( @entity10328 ) reduce @entity738 formation.
