Detection of the MicroRNA expression profile in skeletal muscles of burn XXXX at the early stage in @entity35 .

@entity1568 @entity2495 @entity71266 @entity35 @entity2057 @entity130
BACKGROUND: Severe burn @entity130 are associated with a persistent @entity2495 response, which causes long-term loss of muscle mass that results in a clinical negative balance of @entity1568 and @entity2057 . MicroRNAs (miRNAs) play a critical role in post-transcriptional regulation of gene expression, which negatively regulates gene expression by promoting degradation of target mRNAs or inhibiting their translation. However, the mechanisms of skeletal @entity2057 after severe burn involved in miRNAs still remain unclear. METHODS: In this study, the alterations of miRNAs expression profile in skeletal muscles of thermal @entity35 were detected at an early stage by microarray. All data were presented as mean  SD. Statistical analysis was determined by independent Student's t-test and one-way ANOVA. The significance was all set at p<0.05, and fold change cut-off was 2.0 for microarray. Significant differentially expressed miRNAs were identified through Volcano Plot filtering. Hierarchical clustering was performed using MEV software (v4.6, @entity71266 ). RESULTS: Thousands of miRNAs could be examined in normal and injured tissues, but only 69 of these were significantly upregulated or down-regulated, which could be used to discriminate skeletal muscles of thermal @entity35 from matched tissues. CONCLUSION: The deregulated miRNAs probably play a potential role in the pathogenesis of skeletal @entity2057 in burn @entity130 .
