Detection and quantification of phytotoxic metabolites of Sarocladium oryzae in sheath rot-infected grains of XXXX .

@entity137 @entity38755 @entity24194 @entity41546 @entity5132 @entity579
AIMS: The present study describes the detection and quantification of the Sarocladium oryzae metabolites, @entity41546 and @entity38755 in extracts of @entity579 grains collected from plants infected with sheath rot. It also describes the phytotoxicity of these metabolites on @entity579 seedlings. METHODS AND RESULTS: @entity41546 and @entity38755 in sheath rot-infected @entity579 grains were detected using thin layer chromatography (TLC) and nuclear magnetic resonance (NMR) analyses. On the TLC plates @entity41546 and @entity38755 moved as brownish yellow spots and showed R(F) values of 0.61 and 0.49, respectively. A standard assay curve was developed on the basis of selective @entity137 of @entity41546 towards Calvibacter michiganensis ATCC 2140 and @entity38755 towards @entity5132 1150. The amounts of @entity41546 and @entity38755 on the basis of standard assay curve were 2.2 and 1.75 microg g(-1) of infected seeds. Treatment of IR 36 @entity579 seedlings with metabolites induced @entity24194 and reduced shoot length by 20%, root length by 30% and root number by 7% relative to control. CONCLUSIONS: @entity41546 and @entity38755 were detected in infected @entity579 grains and these metabolites induced @entity24194 and reduced the seed viability and seedling health of @entity579 . SIGNIFICANCE AND IMPACT OF THE STUDY: Antimicrobial and phytotoxic metabolites, @entity41546 and @entity38755 are present in infected grains and reduce the seed viability and seedling health. These metabolites may increase the pathogenic potential and survival of S. oryzae in @entity579 seed by competing with other seed-borne fungi.
