Biofilm production by isolates of Candida species recovered from nonneutropenic XXXX : comparison of bloodstream isolates with isolates from other sources.

@entity1 @entity9841 @entity7408 @entity4963 @entity7409 @entity5132 @entity12045 @entity413
Biofilm production has been implicated as a potential virulence factor of some Candida species responsible for catheter-related @entity12045 in @entity1 receiving parenteral nutrition. We therefore compared clinical bloodstream isolates representing seven different Candida species to each other and to those from other anatomical sites for the capacity to form biofilms in @entity413 -containing medium. Potential associations between the capacity to form biofilms and the clinical characteristics of @entity12045 were also analyzed. Isolates included the following from nonneutropenic @entity1 : 101 bloodstream isolates (35 @entity7409 , 30 @entity5132 , 18 @entity9841 , 8 @entity7408 , and 10 other Candida species isolates) and 259 clinical isolates from other body sites (116 @entity5132 , 53 @entity7408 , 43 @entity9841 , 17 @entity7409 , and 30 other Candida species isolates). Organisms were grown in Sabouraud dextrose broth (SDB) containing a final concentration of 8% @entity413 to induce biofilm formation, as published previously. Biofilm production was determined by both visual and spectrophotometric methods. In this medium, biofilm production by @entity5132 isolates was significantly less frequent (8%) than that by non- @entity5132 Candida species (61%; P < 0.0001). The overall proportion of non- @entity5132 Candida species isolates from the blood that produced biofilms was significantly higher than that of non- @entity5132 Candida isolates obtained from other sites (79% versus 52%; P = 0.0001). Bloodstream isolates of @entity7409 alone were significantly more likely to be biofilm positive than were @entity7409 isolates from other sites (86% versus 47%; P = 0.0032). Non- @entity5132 Candida species, including @entity7409 , were more likely to be biofilm positive if isolates were derived from @entity1 whose @entity4963 was central venous catheter (CVC) related (95%; P < 0.0001) and was associated with the use of total parenteral nutrition (TPN) (94%; P < 0.005). These data suggest that the capacity of Candida species isolates to produce biofilms in vitro in @entity413 -containing SDB may be a reflection of the pathogenic potential of these isolates to cause CVC-related @entity12045 in @entity1 receiving TPN.
