Influence of environmental medium on @entity844 composition of XXXX cells: leukocytes and fibroblasts.

@entity1 @entity778 @entity844 @entity5204 @entity4600 @entity1474 @entity6221 @entity23165 @entity8981 @entity1718 @entity4722 @entity2241
Fibroblasts in culture and leukocytes have been widely used to study @entity844 and lipoprotein cellular metabolism. The present investigations were designed to study the role of nutritional and environmental factors on lipid metabolism in these two types of cells. Leukocytes freshly isolated from @entity1 blood and fibroblasts cultured in media enriched in @entity778 ( @entity778 ) have relatively similar @entity844 distributions. However, more important differences are observed in fibroblasts cultured in media enriched with @entity778 or with @entity2241 ( @entity2241 ). It is obvious that the quantity and quality of @entity1718 are very different in @entity2241 and @entity778 , but intracellular regulation ensures relative homogeneity of saturated (SFA) and @entity6221 ( @entity8981 ) in the cells, particularly in phospholipids. The first modifications induced by different media ( @entity2241 or @entity778 ) are detected on cellular growth; the differences seem to be due more to the @entity844 (FA) quantitative supply than to the FA quality of each culture medium. The major modifications in FA composition induced by different culture media concern the @entity1474 ( @entity5204 ) of phospholipids, especially the n-6 family. The intracellular @entity4722 level depends on the level in the medium, but intracellular n-6 metabolite levels depend both on the level in the medium and on the growth state of the cells. The n-3 family seems to be less affected by the quality of the medium in our experiment, and the cells maintain a stable @entity4600 (22:6n-3) level. A higher content of the n-3 family in the medium induces a higher level of eicosa- or @entity23165 , rather than @entity4600 itself.(ABSTRACT TRUNCATED AT 250 WORDS)
