Intervertebral Disc Cells Produce Interleukins Found in @entity1 with Back XXXX .

@entity1 @entity1356 @entity6977 @entity1635 @entity615 @entity158 @entity687 @entity688
OBJECTIVE: To examine the link between cytokines in intervertebral disc (IVD) tissues and @entity615 . DESIGN: In vitro study with @entity1 IVD cells cultured from cadaveric donors and annulus fibrosus (AF) tissues from @entity1 . RESULTS: Cultured nucleus pulposus (NP) and AF cells were stimulated with @entity1356 . @entity687 and @entity6977 gene expression was analyzed using real-time polymerase chain reaction. @entity687 protein was quantified by enzyme-linked immunosorbent assay. After @entity1356 stimulation, @entity687 gene expression increased 26,541 fold in NP cells and 22,429 fold in AF cells, whereas protein released by the NP and AF cells increased 2,389- and 1,784-fold, respectively. @entity6977 gene expression increased 3.3-fold in NP cells (P < 0.05).Cytokine profiles in AF tissues collected from @entity1 undergoing surgery for @entity615 ( @entity158 group) or @entity1635 (controls) were compared by cytokine array. @entity687 protein in the AF tissues from @entity1 with @entity615 was 1.81-fold of that in controls. @entity6977 and @entity688 in AF tissues from the @entity158 group were 6.87 and 4.63 times greater than the corresponding values in controls, respectively (P < 0.05). CONCLUSION: Inflammatory mediators found in AF tissues from @entity1 with @entity615 are likely produced by IVD cells and may play a key @entity615 .
