[The study on the proliferation and the apoptosis factors in vitro of K  lliker organ supporting cells in the cochlea of newborn XXXX ].

@entity3264 @entity1976 @entity35 @entity12276 @entity2311 @entity98 @entity13909 @entity460 @entity972 @entity141 @entity4124
OBJECTIVE: To study the apoptosis/proliferation of K  lliker organ supporting cells and to understand the prompting apoptosis factors in vivo in the supporting cells in the K  lliker organ by changing the environment of the cultured supporting cells in the Kliker organ in vitro, via the separation, culture and purification of the supporting cells in the K6lliker organ. METHOD: A combinatorial approach of enzymatic digestion and mechanical separation was employed to isolate and culture in vitro pure K  lliker organ supporting cells. The purity was tested by flow cytometry assay. And K6lliker organ supporting cells were harvested to detect the rate and cycle of apoptosis by flow cytometry after @entity13909 /PI staining, to test the cell growth curve by @entity98 assay, and to observe the differential expressions of the @entity1976 , @entity4124 , @entity12276 and @entity2311 through the Realtime PCR and Western blot. The @entity460 , @entity972 and @entity3264 concentrations in the culture medium of these cells in vitro were changed to detect the survival rate of cells by @entity98 assay. RESULT: The purity of K6lliker organ supporting cells by flow cytometry assay was 96. 56%. And these cells showed no significant difference in apoptosis, but an evident linear growth. The results of Realtime PCR and Western blot showed that the expression of @entity1976 , @entity4124 , @entity12276 and @entity2311 mRNA and protein in all different time points kept stable. Furthermore, the elevation of extracellular @entity141 might contribute to decrease the cell viability of supporting cells. And K+ participated regulation of cell viability in a concentration-depending way. However, @entity3264 appeared to be a protective factor in high concentration. CONCLUSION: There is no significant apoptosis in vitro of the supporting cells in the K  lliker organ of @entity35 , showing a linear growth. The @entity141 in high concentration might contribute to the apoptosis factor of these cells. However, the K+ and @entity3264 appear to be protective factors in high concentration.
