XXXX induced by growth factors: intravital microscopic studies using models.

@entity10245 @entity19 @entity1504 @entity1617 @entity1779 @entity934
Pharmacological induction of angiogenesis is a new treatment of @entity1504 without surgical treatment. It is an urgent task to investigate the dynamic process of angiogenesis and of the microvascular perfusion of the cerebral neoplastic tissue in vivo. The present study is concerned with microcirculatory aspects of @entity1779 in vivo. A novel model of cerebral angiogenesis was developed by inducing @entity1779 in @entity19 using growth factors such as @entity10245 ( @entity10245 ) and platelet-derived growth factor (PDGF). By a direct observation of the neocapillary microcirculation under a fluorescence videomicroscope, the neocapillary density, diameter and red cell velocity were measured to evaluate the development and remodeling of the neocapillaries with the number of days after incubation. The neocapillary response to topically applied @entity934 ( @entity1617 ) was examined by measuring changes in the diameter and red cell velocity. It was shown that PDGF-induced neocapillaries was dilated in response to ACh on day 28 after incubation, while @entity10245 -induced neocapillaries was not. Furthermore, the neocapillary pericytes were observed using confocal laser microscopy, based on the fluorescence immunohistochemical images of the neoplastic tissue. Several pericytes, stained with anti-NG_2, appeared in the neocapillaries. It was suggested that these pericytes might be recruited in the neocapillaries to regulate blood flow without vascular smooth muscle.
