Nuclear factor-kappaB activation in peripheral blood mononuclear cells in XXXX with @entity295 .

@entity1 @entity650 @entity295 @entity404 @entity5750 @entity5 @entity39 @entity1199 @entity3646 @entity688 @entity405
OBJECTIVE: To determine the activation of nuclear factor-[kappa]B in peripheral blood @entity5750 + monocyte/macrophages and CD3+, @entity404 +, and @entity405 + T cells in @entity1 with @entity295 . DESIGN: Observational study. SETTING: University hospital. @entity1 : Twenty-six @entity1 with @entity295 (nine females and 17 males, aged between 10 days and 15 yrs; median, 4.3 yrs) on admission to our hospital between August 1999 and November 2005. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: The percentages of peripheral blood @entity5750 + monocyte/macrophages and CD3+, @entity404 +, and @entity405 + T cells exhibiting nuclear factor-[kappa]B activity were determined by flow cytometry. In addition, relationships among the degree to which nuclear factor-[kappa]B was activated, serum levels of cytokines (interferon-[gamma], @entity5 @entity39 factor-[alpha], @entity1199 , @entity3646 , @entity650 , and @entity688 ), and clinical variables were analyzed. The percentage of cells exhibiting nuclear factor-[kappa]B activity was increased among @entity5750 +, CD3+, @entity404 +, and @entity405 + cells in the @entity295 group and was significantly higher among @entity5750 +, CD3+, and @entity404 + cells of the @entity1 with severe @entity295 (n = 9) than those of @entity1 with nonsevere @entity295 (n = 17). The percentage of cells exhibiting nuclear factor-[kappa]B activity was significantly higher among @entity5750 + cells than CD3+ cells in the @entity1 with severe @entity295 . In addition, this percentage was significantly higher among @entity5750 + cells than CD3+, @entity404 +, and @entity405 + cells in septic @entity1 who had positive blood cultures (n = 16). Serum @entity650 levels were correlated with the percentages of @entity5750 +, CD3+, @entity404 +, and @entity405 + cells exhibiting nuclear factor-[kappa]B activity, and serum @entity688 levels were correlated with the percentages of @entity5750 +, CD3+, and @entity404 + cells exhibiting nuclear factor-[kappa]B activity. CONCLUSIONS: Nuclear factor-[kappa]B in peripheral blood mononuclear cells was activated in @entity1 with @entity295 and was related the severity of @entity295 .
