Molecular cloning of a XXXX cDNA encoding a novel protein, p @entity19 G4, which varies with the cell cycle.

@entity19 @entity6739 @entity8270 @entity535
Proliferating cells express genes active in cell cycle control. The modulation of control genes and factors are required to maintain critical cell cycle activities. We used a set of monoclonal antibodies prepared against DNA-binding proteins from @entity8270 cells in immunofluorescent microscopy to screen for proteins showing cell cycle-specific staining patterns. Here, we report cloning and characterizing of a novel mitogen-inducible gene from @entity19 macrophages that predicts a cell cycle-specifically modulated nuclear protein of 38 kDa, designated p38-2G4. p38-2G4 displayed a speckled pattern of varying fluorescence intensity confined to the nucleus, but sparing the nucleoli. Strongly stained granules were observed between G1 and mid S phase, followed by a less abundant punctated arrangement toward the end of S phase, and negative fluorescence at the S/G2 transition. Thereafter, the nuclear staining reappeared. Additionally, p38-2G4 expression vanished in G0-arrested cells and was restored after release from growth arrest. p38-2G4 conserved in vertebrates by means of immunofluorescence data contains a number of putative phosphorylation sites, a cryptic nuclear localization signal, and an amphipathic helical domain. Our cDNA and its deduced @entity535 sequence is related to a @entity6739 gene encoding a 42-kDa protein that associates with curved DNA, suggesting that we have cloned the @entity19 homologue of the @entity6739 gene which defines a novel cell cycle-specifically modified and proliferation-associated nuclear protein in mammals.
