Metabolic flux analysis of Escherichia coli MG1655 under XXXX (C8) stress.

@entity6212 @entity7574 @entity7352 @entity2974 @entity3158 @entity598 @entity6626 @entity505 @entity2388 @entity413 @entity526
UNASSIGNED: Systems metabolic engineering has made the renewable production of industrial chemicals a feasible alternative to modern operations. One major example of a renewable process is the production of @entity7574 , such as @entity6212 (C8), from @entity526 , engineered to express thioesterase enzymes. C8, however, is toxic to @entity526 above a certain concentration, which limits the final titer. @entity7352 metabolic flux analysis of @entity526 was performed for both C8 stress and control conditions using NMR2Flux with isotopomer balancing. A mixture of labeled and unlabeled @entity413 was used as the sole @entity505 source for bacterial growth for @entity7352 flux analysis. By comparing the metabolic flux maps of the control condition and C8 stress condition, pathways that were altered under the stress condition were identified. C8 stress was found to reduce @entity505 flux in several pathways: the @entity6626 ( @entity2974 ) cycle, the @entity3158 production, and the @entity2388 dehydrogenase pathway. Meanwhile, a few pathways became more active: the @entity2388 oxidative pathway, and the extracellular @entity598 production. These results were statistically significant for three biological replicates between the control condition and C8 stress. As a working hypothesis, the following causes are proposed to be the main causes for growth inhibition and flux alteration for a cell under stress: membrane disruption, low activity of electron transport chain, and the activation of the @entity2388 dehydrogenase regulator (PdhR).
