Modulation of inhibitory @entity2228 receptors in cultured embryonic @entity19 hippocampal neurons by @entity219 , @entity1432 containing redox agents and XXXX .

@entity8862 @entity1 @entity2226 @entity2228 @entity19 @entity3793 @entity8865 @entity219 @entity2041 @entity3902 @entity11057 @entity1432 @entity9880 @entity13201 @entity48076
Modulation of inhibitory @entity2228 receptors by @entity219 ( @entity8862 ) and endogenous redox agents such as @entity2041 may alter inhibition in the @entity1 brain. Despite the abundance of @entity8862 in the hippocampus and its ability to modulate @entity2228 receptors, few studies have examined @entity8862 modulation of hippocampal @entity2228 receptors. Whether redox agents modulate hippocampal @entity2228 receptors also remains unknown. This study examined @entity8862 and redox modulation of @entity2228 receptor-mediated currents in cultured embryonic @entity19 hippocampal neurons using whole-cell recordings. @entity8862 concentrations below 10 microM potentiated currents elicited by low @entity2228 , @entity11057 , and @entity2226 concentrations by 300-400%. @entity8862 concentrations above 300 microM produced nearly complete inhibition. Potentiating @entity8862 concentrations shifted the dose-response curves for the three agonists to the left and decreased the Hill coefficient for @entity2228 and @entity11057 but not @entity2226 . Inhibiting @entity8862 concentrations shifted the dose-response curves for @entity2228 and @entity11057 to the right but reduced the maximum @entity2226 response. @entity3793 residues may participate in potentiation because @entity13201 and pH 5.4 diminished @entity8862 enhancement of @entity2228 currents. pH 5.4 diminished @entity8862 block of @entity2228 currents, but @entity13201 did not. These findings indicate that separate sites mediate @entity8862 potentiation and inhibition. The redox agents @entity2041 , @entity3902 , @entity48076 , and @entity8865 did not alter @entity2228 currents by a redox mechanism. However, @entity2041 and @entity3902 interfered with the effects of @entity8862 on @entity2228 currents by chelating it. @entity9880 had similar effects. Thus, @entity8862 and @entity1432 containing redox agents that chelate @entity8862 modulate hippocampal @entity2228 receptors with the mechanism of @entity8862 modulation being agonist dependent.
