Endoplasmic reticulum stress involved in high-fat diet and @entity7609 -induced vascular damages and XXXX intervention.

@entity3724 @entity19 @entity12099 @entity35 @entity7609
UNASSIGNED: @entity3724 (FF) is widely used to lower blood lipids in clinical practice, but whether its protective effect on endothelium-dependent vasodilatation (EDV) in thoracic aorta is related with endoplasmic reticulum (ER) stress remains unknown. In this study, female Sprauge Dawley @entity35 were divided into standard chow diets (SCD), high-fat diets (HFD) and HFD plus FF treatment group (HFD  +  FF) randomly. The @entity35 of latter two groups were given HFD feeding for 5 months, then HFD  +  FF @entity35 were treated with FF (30  mg/kg, once daily) via gavage for another 2 months. The pathological and tensional changes, protein expression of eNOS, and ER stress related genes in thoracic aorta were measured. Then impacts of @entity7609 (PA) and FF on EDV of thoracic aorta from normal female SD @entity35 were observed. Ultimately the expression of ER stress related genes were assessed in primary @entity19 aortic endothelial cells (MAEC) treated by @entity12099 (FA) and PA. We found that FF treatment improved serum lipid levels and pathological changes in thoracic aorta, accompanied with decreased ER stress and increased phosphorylation of eNOS. FF pretreatment also improved EDV impaired by different concentrations of PA treatment. The dose- and time-dependent inhibition of cell proliferation by PA were inverted by FA pretreatment. Phosphorylation of eNOS and expression of ER stress related genes were all inverted by FA pretreatment in PA-treated MAEC. Our findings shows that @entity3724 recovers damaged EDV by chronic HFD feeding and acute stimulation of PA, this effect is related with decreased ER stress and increased phosphorylation of eNOS.
