Basis for defective responses of XXXX synovial fluid lymphocytes to anti-CD3 (T3) antibodies.

@entity1 @entity3647 @entity308 @entity1192 @entity1199 @entity309
Synovial fluid mononuclear cells (SFMC) from @entity1 with @entity309 characteristically respond poorly to mitogens. In this study, mitogenic antibodies reactive with the CD3(T3) antigen on @entity1 T lymphocytes were used to analyze the basis for the deficiency. OKT3-induced proliferation and release of interleukin 1 ( @entity1192 ) and interleukin 2 ( @entity1199 ) from SFMC were @entity308 in all @entity1 . Purified @entity1192 or recombinant @entity1199 restored proliferative responses in SFMC and increased @entity3647 density. Exogenous @entity1192 also enhanced @entity1199 release. Fractionation of SFMC supernatants on phosphocellulose columns revealed the presence of @entity1192 and a potent @entity1192 inhibitor. The monocyte-derived @entity1192 inhibitor blocked @entity1192 -dependent responses of normal peripheral blood lymphocytes to OKT3, but had no effect on @entity1199 -dependent events. These results suggest that @entity1192 inhibitor(s) in SFMC impair(s) OKT3-induced mitogenesis by interfering with the effects of @entity1192 on T lymphocytes. The net result is deficient @entity1199 secretion, @entity3647 expression, and impaired cellular proliferation. This novel inhibitory circuit provides a rational explanation for the diminished function of synovial fluid T lymphocytes in @entity309 @entity1 .
