Small RNA Sequencing Uncovers New miRNAs and moRNAs Differentially Expressed in Normal and Primary Myelofibrosis XXXX + Cells.

@entity1 @entity6582 @entity9656 @entity5 @entity4407 @entity3052 @entity4018 @entity1583 @entity2280 @entity32313
@entity5 ( @entity5 ) are chronic @entity5 thought to arise at the level of @entity2280 + hematopoietic stem/progenitor cells. They include @entity4407 ( @entity4407 ), @entity6582 vera (PV) and @entity4018 ( @entity4018 ). All can progress to @entity3052 , but @entity4018 carries the worst prognosis. Increasing evidences indicate that deregulation of microRNAs (miRNAs) might plays an important role in @entity1583 , including @entity5 . To attain deeper knowledge of short RNAs (sRNAs) expression pattern in @entity2280 + cells and of their possible role in mediating post-transcriptional regulation in @entity4018 , we sequenced with Illumina HiSeq2000 technology @entity2280 + cells from healthy subjects and @entity4018 @entity1 . We detected the expression of 784 known miRNAs, with a prevalence of miRNA up-regulation in @entity4018 samples, and discovered 34 new miRNAs and 99 new miRNA-offset RNAs (moRNAs), in @entity2280 + cells. Thirty-seven small RNAs were differentially expressed in @entity4018 @entity1 compared with healthy subjects, according to microRNA sequencing data. Five miRNAs (miR-10b-5p, miR-19b-3p, miR-29a-3p, miR-379-5p, and @entity32313 ) were deregulated also in @entity4018 granulocytes. Moreover, 3'-moR-128-2 resulted consistently downregulated in @entity4018 according to RNA-seq and qRT-PCR data both in @entity2280 + cells and granulocytes. Target predictions of these validated small RNAs de-regulated in @entity4018 and functional enrichment analyses highlighted many interesting pathways involved in @entity5 development and progression, such as signaling by FGFR and @entity9656 and Oncogene Induced Senescence. As a whole, data obtained in this study deepened the knowledge of miRNAs and moRNAs altered expression in @entity4018 @entity2280 + cells and allowed to identify and validate a specific small RNA profile that distinguishes @entity4018 granulocytes from those of normal subjects. We thus provided new information regarding the possible role of miRNAs and, specifically, of new moRNAs in this disease.
