Inhibition of inducible @entity842 synthase and cyclooxygenase-2 activity by XXXX in murine macrophage cells.

@entity32 @entity10178 @entity16538 @entity22215 @entity5 @entity1421 @entity3089 @entity3090 @entity15039 @entity842 @entity13633 @entity105 @entity70656 @entity11676 @entity22216
Activated macrophages express inducible isoforms of @entity842 synthase ( @entity13633 ) and cyclooxygenase ( @entity10178 ), and produce excessive amounts of @entity842 (NO) and @entity1421 ( @entity1421 ), which play key roles in the processes of @entity32 and @entity105 . The root of Paeonia lactiflora Pall., and the root cortex of Paeonia suffruticosa Andr., are important Chinese crude drugs used in many traditional prescriptions. @entity22215 ( @entity22216 ) is a major bioactive constituent of both crude drugs. @entity22216 has been shown to possess potent anti-oxidant, anti-mutagenic, anti-proliferative and anti-invasive effects. In this study, we examined the inhibitory effects of @entity22215 ( @entity22216 ) isolated from the root of Paeonia lactiflora Pall. on the @entity10178 and @entity13633 activity in LPS-activated Raw 264.7 cells, @entity16538 in HEL cells. To investigate the structure-activity relationships of gallate and @entity11676 for the inhibition of @entity13633 and @entity10178 activity, we also examined @entity3089 ( @entity3090 ), @entity11676 , and @entity70656 . The results of the present study indicated that @entity22216 , @entity3090 , and @entity70656 treatment except @entity11676 significantly inhibited LPS-induced NO production in LPS-activated macrophages. All of the four compounds significantly inhibited @entity10178 activity in LPS-activated macrophages. Among the four compounds examined, @entity22216 revealed the most potent in both @entity13633 (IC50 approximately 18 microg/mL) and @entity10178 inhibitory activity ( @entity1421 : IC50 approximately 8 microg/mL and @entity15039 : IC50 approximately 12 microg/mL), respectively. Although further studies are needed to elucidate the molecular mechanisms and structure-activity relationship by which @entity22216 exerts its inhibitory actions, our results suggest that @entity22216 might be a candidate for developing anti-inflammatory and @entity5 chemopreventive agents.
