Plasma-mediated neutrophil activation during XXXX : role of platelet-activating factor.

@entity1 @entity296 @entity26 @entity1196 @entity3766 @entity1454 @entity50549 @entity9536 @entity583 @entity224
1. Polymorphonuclear neutrophils are involved in the development of @entity296 during ischaemia through the release of free @entity26 radicals and by adhesion of activated polymorphonuclear neutrophils to endothelium, resulting in plugging of coronary capillaries. Polymorphonuclear neutrophil activation may be a result of contact with ligands expressed by endothelial cells and/or a response to soluble stimuli released from ischaemic tissue to the plasma. 2. To investigate this we studied plasma-mediated polymorphonuclear neutrophil activation in vitro using plasma samples collected from 14 @entity1 with @entity583 at time of admission and 6 h and 1, 2, 5 and 7 days later. Plasma samples were incubated with washed polymorphonuclear neutrophils isolated from healthy donors. Expression of adhesion molecules @entity1454 / @entity9536 integrin and @entity1196 ( @entity1196 ) were measured by flow cytometry and @entity224 anion production in polymorphonuclear neutrophils was measured by chemiluminescence. 3. Plasma samples obtained 6 h and 1 day after admission were capable of inducing @entity1454 / @entity9536 antigen expression, @entity224 anion production and @entity1196 shedding in the washed polymorphonuclear neutrophils, and this effect was significant when compared with plasma taken at 5 and 7 days after admission. 4. The plasma-mediated polymorphonuclear neutrophil stimulation was prevented when the PMN were pretreated with platelet-activating factor receptor antagonists @entity3766 or @entity50549 . The platelet-activating factor concentrations detected in the plasma samples were not higher than those detected in plasma from healthy subjects.(ABSTRACT TRUNCATED AT 250 WORDS)
