Immunocytochemical markers in XXXX diagnosis.

@entity1 @entity981 @entity3052 @entity1582 @entity1778 @entity10706 @entity11511
The study included 1742 @entity1 with @entity3052 ( @entity3052 ) and @entity981 ( @entity981 ), Kyiv city residents and @entity1 from 20 regions of Ukraine. Bone marrow and blood smears were sent at diagnosis to Reference Center. The analysis was based on May-Gr  nvald-Giemza (MGG) stain and cytochemical reactions (MPO, acNSE, CAE, AP, PAS). Immunocytochemical techniques (APAAP, LSAB) and broad panel of monoclonal antibodies (MoAbs) against lineage specific and differentiation antigens of leukocytes were employed for immunophenotyping of leukemic blast cells directly in blood and bone marrow smears. Different types of @entity3052 were defined by the expression of the cell surface and cytoplasmic antigens. Immunocytochemical study was required especially in diagnosing of @entity3052 with minimal differentiation, @entity10706 , @entity1778 and @entity1778 of ambiguous lineage. @entity981 was broadly classified into B-lineage and @entity11511 . According to the degree of B-lymphoid differentiation of the blast cells four subtypes of B-lineage @entity981 were established. @entity11511 observed in @entity1 were also divided into four subtypes. Immunocytochemical examination was required to diagnose @entity1582 of ambiguous lineage with no clear evidence of lineage differentiation ( @entity1778 ) or those with blasts that express markers of more than one lineage (mixed phenotype @entity1778 ).
