Functional marker development of XXXX -InDel and allelic diversity within the genus Glycine.

@entity286 @entity73138 @entity2036 @entity1460 @entity579
BACKGROUND: Single-stranded non-protein coding small RNAs, 18-25 @entity2036 in length, are ubiquitous throughout plants genomes and are involved in post-transcriptional gene regulation. Several types of DNA markers have been reported for the detection of genetic diversity or sequence variation in @entity1460 , one of the most important legume crops in worldwide for seed protein and oil content. Recently, with the available of public genomic databases, there has been a shift from the labor-intensive development of PCR-based markers to sequence-based genotyping and the development of functional markers within genes, often coupled with the use of RNA information. But thus far miRNA-based markers have been only developed in @entity579 and @entity286 . Here we report the first functional molecular miRNA marker, @entity73138 -InDel, in @entity1460 for a specific single copy locus used to assess genetic variation in domesticated @entity1460 ( @entity1460 ) and its wild progenitor (Glycine soja Sieb. _ Zucc.). RESULTS: We genotyped a total of 1,669 accessions of domesticated @entity1460 (G. max) and its wild progenitor G. soja which are native throughout the China and parts of Korea, Japan and Russia. The results indicate that the @entity73138 locus is distributed in cultivated @entity1460 and has three alleles in annual wild @entity1460 . Based on this result, we proposed that miR-InDel marker technology can be used to assess genetic variation. The inclusion of geo-reference data with @entity73138 -InDel marker data corroborated that accessions from the Yellow River basin (Huanghuai) exhibited high genetic diversity which provides more molecular evidence for gene diversity in annual wild @entity1460 and domestication of @entity1460 . CONCLUSIONS: These results provide evidence for the use of RNA marker, miRNA1511-InDel, as a @entity1460 -specific functional maker for the study of genetic diversity, genotyping of germplasm and evolution studies. This is also the first report of functional marker developed from @entity1460 miRNA located within the functional region of pre-miRNA1511.
