A @entity19 model of XXXX induced with @entity8629 and lipopolysaccharide.

@entity1 @entity32 @entity788 @entity656 @entity39 @entity19 @entity4 @entity2617 @entity548 @entity7031 @entity1234 @entity842 @entity8629 @entity224 @entity1865
AIM: To establish a non-traumatic, easy to induce and reproducible @entity19 model of @entity656 ( @entity656 ) induced with @entity8629 and lipopolyasccharide (LPS). METHODS: Thirty-two healthy mature NIH female @entity19 were selected and divided at random into four groups (each of 8 @entity19 ), i.e., the control group ( @entity7031 group), the @entity8629 group (Cn group), the lipopolysaccharide group (LPS group), and the @entity8629 +LPS group (Cn+LPS group). @entity19 were injected intraperitoneally with @entity8629 only, or LPS only, and @entity8629 and LPS in combination. All the animals were then killed by neck dislocation three hours after the last intraperitoneal injection. The pancreas and exo-pancreatic organs were then carefully removed for microscopic examination. And the pancreatic acinus was further observed under transmission electron microscope (TEM). Pancreatic weight, serum amylase, serum @entity842 (NO) concentration, @entity224 dismutase (SOD) and @entity1234 ( @entity1865 ) concentration of the pancreas were assayed respectively. RESULTS: (1) @entity7031 animals displayed normal pancreatic structure both in the exocrine and endocrine. In the LPS group, the pancreas was slightly @entity4 , with the @entity2617 of a few inflammatory cells and the @entity39 of the adjacent fat tissues. All the animals of the Cn group showed distinct signs of a mild @entity788 characterized by @entity4 , @entity2617 of neutrophil and mononuclear cells, but without obvious parenchyma @entity39 and @entity548 . In contrast, the Cn+LPS group showed more diffuse focal areas of nonviable pancreatic and @entity548 as well as systemic organ dysfunction. According to Schmidt's criteria, the pancreatic histologic score showed that there existed significant difference in the Cn+LPS group in the @entity4 , @entity32 , parenchyma @entity39 and parenchyma homorrhage in comparison with those of the Cn group, LPS group and @entity7031 group (P<0.01 or P<0.05). (2) The ultrasturcture of acinar cells was seriously damaged in the Cn+LPS group. Chromatin margination of nuclei was present, the number and volume of vacuoles greatly increased. Zymogen granules (ZGs) were greatly decreased in number and endoplasmic reticulum exhibited whorls. The swollen mitochondria appeared, the crista of which was decreased in number or disappeared. (3) Pancreatic weight and serum amylase levels in the Cn +LPS was significantly higher than those of the @entity7031 group and the LPS group respectively (P<0.01 or P<0.05). However, the pancreatic wet weight and serum amylase concentration showed no significant difference between the Cn+LPS group and the Cn group. (4) NO concentration in the Cn+LPS group was significantly higher than that of @entity7031 group, LPS group and Cn group(P<0.05 or P<0.01). (5) The SOD and @entity1865 concentration of the pancreas in the Cn+LPS group were significantly higher than those of @entity7031 , LPS and Cn groups (P<0.05 or P<0.01). CONCLUSION: The @entity19 model of @entity656 could be induced with @entity8629 and LPS, which could be non-traumatic and easy to induce, reproducible with the same pathological characteristics as those of @entity656 in @entity1 , and could be used in the research on the mechanism of @entity1 @entity656 .
