Monoclonal antibodies to @entity60 inhibit XXXX -dependent induction of growth and maturation in lectin/antigen-reactive cytolytic T lymphocyte precursors.

@entity1 @entity2047 @entity19 @entity5 @entity60 @entity1199 @entity3414
In this study we tested the effect of monoclonal antibodies (moAb) AN-18 to @entity19 @entity60 on the generation of cytolytic T cells (CTL) from a homogeneous population of precursor cells (CTL-P). As responder cells, highly purified @entity3414 + C57BL/6 lymph node T cells were used that had been positively selected by flow cytofluorometry on a cell sorter. @entity3414 + cells were set up in bulk culture or in limiting dilution (LD) either with Con A or with @entity19 @entity5 cells as antigen and recombinant @entity1 @entity1199 (rec. @entity1199 ) in the presence or absence of moAb AN-18 and tested for growth and development of CTL. The results show that moAb AN-18 but not the unrelated moAb AN-37 diminished or abrogated proliferative and cytolytic responses of @entity3414 + lymphocytes to lectin and rec. @entity1199 in a dose-dependent manner. The inhibitory activity of the antibodies could be abolished by neutralizing moAb AN-18 with recombinant @entity19 @entity60 (rec.mIFN-gamma) before their addition to culture. Kinetic analysis shows that the inhibitory effect of moAb AN-18 is only optimal when added at the beginning of culture or up to 48 hr after initiation. The frequencies of CTL-P responding either to Con A or to @entity19 @entity5 cells and rec. @entity1199 were reduced up to 10-fold in the presence of moAb AN-18. The inhibitory capacity of moAb AN-18 was also operative in cultures containing on the average one antigen-specific CTL-P. Together with the finding that activated CTL-P secrete @entity60 in response to rec. @entity1199 in a dose-dependent manner, the data suggest that endogenous @entity60 collaborates with exogenous @entity2047 in the induction of CTL-P. The generation of CTL may therefore represent a case of autocrine growth regulation of normal lymphocytes, in which the same cell synthesizes and responds to its own factor.
