Sarcocystis spp. Infection in two @entity70566 Cubs ( XXXX ).

@entity37241 @entity2878 @entity1579 @entity70566 @entity3204
UNASSIGNED: Two neonatal male @entity70566 ( @entity70566 ) littermates were submitted for necropsy examination. One animal was found dead with no prior signs of illness; the other had a brief history of laboured breathing. Post-mortem examination revealed disseminated @entity2878 . To further characterize the causative agent, @entity1579 ( @entity1579 ), immunohistochemistry (IHC), polymerase chain reaction (PCR) and amplification and nucleic acid sequencing were performed. IHC was negative for @entity3204 and Neospora caninum, but was positive for a Sarcocystis spp. @entity1579 of cardiac muscle and lung revealed numerous intracellular apicomplexan protozoa within parasitophorous vacuoles. PCR and nucleic acid sequencing of partial 18S rRNA and the internal transcribed spacer (ITS)-1 region confirmed a Sarcocystis spp. that shared 99% sequence homology to Sarcocystis neurona and Sarcocystis dasypi. This represents the first report of @entity37241 in @entity70566 . The histopathological, immunohistochemical, molecular and ultrastructural findings are supportive of vertical transmission resulting in fatal disseminated disease.
