Inactivation of model viruses suspended in fresh frozen plasma using novel XXXX based device.

@entity1 @entity8310 @entity6693 @entity4376 @entity3307 @entity291
BACKGROUND AND OBJECTIVE: There is a concern on safety of @entity1 Fresh Frozen Plasma (FFP) as it is a source of some medicinal products. The possibility of transmission of blood-borne are reported often due to emerging viruses. There are some Pathogen Reduction Technologies (PRT) to inactivate viruses. @entity6693 (MB) based method is one of them. The aim of this study was to examine new designated device to inactivate model viruses. MATERIALS AND METHODS: Four model viruses were used in this study: @entity8310 virus (VSV), Herpes Simplex Virus I ( @entity3307 ), @entity291 Viral DiarrheaVirus( @entity4376 ) and Polio Virus.50% Tissue Culture Infective Dose (TCID 50) and Reed-Muench Methods were used to titer the viruses. MB in two final concentration of 0.1  M and 1  M and illumination in about 627nm with red LED (Lamp Emitting Diode) for 15, 30, 45 and 60 minutes were used. Three replicates employed for each experiments. RESULTS: 1 Mconcentration of MB showed more effective than 0.1 Min all designed illumination period for inactivation of HSV, VSV and @entity4376 . This method also demonstrated best results for enveloped model viruses. The most Log reduction for HSV, VSV and @entity4376 were6.28, 5.54 and 6.22, respectively. For HSV and @entity4376 inactivation, the best illumination period was 45 minutes. CONCLUSION: Model viruses showed sensitivity combination of MB and illumination using red LEDs. As results show this device could inactivate model viruses and reduce their titer very close to approved commercial devices, in compare.
