Frequent induction of XXXX in in vivo skin fibroblasts after allogeneic @entity8 : hints to chromosomal instability after irradiation.

@entity1 @entity8 @entity5 @entity348 @entity2008
BACKGROUND: @entity348 ( @entity348 ) has been part of standard conditioning regimens before allogeneic @entity8 for many years. Its effect on normal tissue in these @entity1 has not been studied extensively. METHOD: We studied the in vivo cytogenetic effects of @entity348 and high-dose chemotherapy on skin fibroblasts from 35 allogeneic @entity8 ( @entity8 ) @entity1 . Biopsies were obtained prospectively (n   =   18 @entity1 ) before, 3 and 12  months after allogeneic @entity8 and retrospectively (n   =   17 @entity1 ) 23-65 months after @entity8 for G-banded chromosome analysis. RESULTS: Chromosomal aberrations were detected in 2/18 @entity1 (11  %) before allogeneic @entity8 , in 12/13 @entity1 (92  %) after 3  months, in all @entity1 after 12  months and in all @entity1 in the retrospective group after allogeneic @entity8 . The percentage of aberrant cells was significantly higher at all times after allogeneic @entity8 compared to baseline analysis. Reciprocal translocations were the most common aberrations, but all other types of stable, structural @entity2008 were also observed. Clonal aberrations were observed, but only in three cases they were detected in independently cultured flasks. A tendency to non-random clustering throughout the genome was observed. The percentage of aberrant cells was not different between @entity1 with and without secondary @entity5 in this study group. CONCLUSION: High-dose chemotherapy and @entity348 leads to severe chromosomal damage in skin fibroblasts of @entity1 after @entity8 . Our long-term data suggest that this damage increases with time, possibly due to in vivo radiation-induced chromosomal instability.
