[Localization and quantitative detection of matrix metalloproteinase in XXXX coronal dentine].

@entity1 @entity17944 @entity3334 @entity28405 @entity1476 @entity2822 @entity71872 @entity1549 @entity12662 @entity15989 @entity1495 @entity5952
OBJECTIVE: To compare the distribution and concentrations of @entity71872 in @entity1 coronal dentin. METHODS: The localization of five types of MMP was performed using immunohistochemistry. Molars were demineralized and sectioned into 5   m thick specimens. All specimens were randomly divided into five groups according to the antibodies. Each group contained two subgroups (n = 6). Immunoreactivity of each subgroup was visualized with @entity15989 solution or @entity2822 and observed under microscopy respectively. Molars were sectioned into slices. The slices were divided into two groups according to superficial or deep dentin and pulverized to fine powder. After dentin protein was extracted, the concentrations of @entity17944 , 8, 9 were detected by using fluorescent microsphere immunoassay. RESULTS: Immunohistochemical staining revealed that @entity28405 were highly concentrated in the deep dentin. However, intense immunoreactivities of @entity1476 , 8, 9 were identified in a 6-10   m wide zone adjacent to the dentino-enamel junction. The content of @entity3334 in superficial layer and deep layer of dentin were (0.037  0.025) and (0.433  0.089) ng/mg. The content of @entity1476 in superficial layer and deep layer of dentin were (0.445  0.115) and (2.730  0.712) ng/mg. The content of @entity1549 in superficial layer and deep layer of dentin were (0.071  0.069) and (0.460  0.108) ng/mg. The content of @entity5952 in superficial layer and deep layer of dentin were (0.586  0.246) and (6.159  0.948) ng/mg. The content of @entity1495 in superficial layer and deep layer of dentin were (0.384  0.185) and (1.460  0.251) ng/mg. The concentrations of all tested @entity12662 were significantly higher in deep dentin than those in superficial dentin (P < 0.05). CONCLUSIONS: There are five types of MMP contained in @entity1 coronal dentin, and the distribution of MMP shows a decreasing trend from the deep dentin to the superficial dentin.
