Identification of endonuclease domain-containing 1 gene in @entity2386 XXXX .

@entity3534 @entity2386 @entity406 @entity29334 @entity3689 @entity64166 @entity526
UNASSIGNED: The mRNA level of the endonuclease domain-containing 1 gene (Jf_ENDOD1) in @entity2386 @entity2386 kidney was significantly increased after injection of @entity406 -killed bacteria cells (FKC) in the previous microarray study. ENDOD1 is a member of the DNA/RNA non-specific nucleases family, and its role in fish immunity has not been reported. The open reading frame of Jf_ENDOD1 cDNA was 912  bp, encoding 303 @entity3534 . The first 27 @entity3534 were predicted to be a signal peptide and the mature Jf_ENDOD1 was calculated as 32  kDa. The @entity3689 sequence of Jf_ENDOD1 showed 76% identity to that of @entity64166 @entity64166 . Transcripts of Jf_ENDOD1 were marginally detected in all sampled tissues from healthy fish, while they were significantly detected in brain, kidney, spleen and intestine at 6  h post FKC injection. Jf_ENDOD1 recombinant protein produced in @entity526 showed @entity29334 activity. Furthermore, to evaluate the @entity29334 activities in  vivo, total proteins from @entity2386 kidney and spleen were extracted at 12, 24 and 72  h post Edwardsiellatarda FKC injection. The @entity29334 activity of extracted protein was higher in treated fish than in untreated fish. Since the mRNA levels were significantly up-regulated after the FKC treatment, Jf_ENDOD1 might be responsible for the activities.
