Exogenous XXXX as a mediator in the regulation of @entity12638 growth and silk gland genome.

@entity12638 @entity1426 @entity2348 @entity2918 @entity3009 @entity3774
@entity2918 are locally acting hormones that have remarkable variety of physiological functions. They are rapidly synthesized in several types of vertebrate cells as oxygenated metabolites of @entity1426 in response to various stimuli. In many insect species they are biosynthesized in fat body and hemocytes mainly in response to @entity2348 . In the present study, we administered synthetic analog of @entity3774 , the most prominent of the @entity2918 to the 48 h old fifth instar @entity12638 , @entity12638 L. at a single dose of 4 microg per larva to study its effects on the larval growth pattern and silk synthesis. The possible role of PGF2alpha at altering the quantum of silk synthesis by controlling the silk gene expression was also studied. The genomic DNA was isolated from the posterior silk gland on Days 5 and 7 of the fifth instar from the @entity3009 treated and the control larvae and were random amplified with arbitrary primers. The result presented notable variation in the amplified product suggesting the participation of PGF2alpha in the silk biosynthesis controlling the silk gene expression. The feeding period of treated larvae was unaffected while the cocoon characters exhibited considerable improvement. The filament traits also were improved notably in the treated larvae. The participation of PGF2alpha analog in the silk biosynthetic process with its physiological and molecular implications are discussed.
