Protection from XXXX induced by combined @entity26 and @entity413 deprivation in organotypic hippocampal cultures by @entity2227 receptor antagonists.

@entity1400 @entity2227 @entity137 @entity1399 @entity63 @entity2667 @entity26 @entity18713 @entity4176 @entity143 @entity140 @entity528 @entity3390 @entity413
Organotypic hippocampal cultures were exposed to defined periods (30 and 60 min) of combined @entity26 and @entity413 deprivation, mimicking transient @entity63 conditions. The involvement of different @entity2227 receptors in individual hippocampal subfields ( @entity1399 , @entity1400 and dentate gyrus) was studied using antagonists of @entity143 ( @entity3390 ) and @entity140 / @entity4176 receptors (CNQX and @entity18713 ). Staining with the fluorescent dye @entity2667 (PI) allowed detection of damaged cells. For quantitative determination of @entity528 , fluorescence intensity was measured after a 22 h recovery period and was related to maximal fluorescence intensity measured after fixation and PI restaining of the cultures at the end of the experiment. @entity3390 (10 microM), CNQX (100 microM) and @entity18713 (100 microM) provided complete protection in @entity1399 , @entity1400 and dentate gyrus following the moderate @entity63 insult, when the antagonists were present permanently. This indicates that none of the ionotropic @entity2227 receptor subtypes dominated @entity137 in the most sensitive subpopulation of neurons. When applied only during the recovery period protection with @entity3390 (10 microM) or CNQX (100 microM) was drastically reduced by about 60% in the most sensitive area ( @entity1399 ), but only slightly by 15% in @entity1400 . Therefore the onset of irreversible damage seems to occur earlier in @entity1399 than in @entity1400 . Blockade of @entity140 / @entity4176 receptors by @entity18713 (100 microM) offered no neuroprotection if the compound was applied only during the recovery period.(ABSTRACT TRUNCATED AT 250 WORDS)
