Association of @entity251 with @entity172 abolishes the beneficial effects of XXXX treatment by increasing ROS generation in aorta endothelial cells.

@entity1 @entity172 @entity251 @entity1641 @entity101 @entity12824 @entity26 @entity1808 @entity409 @entity11934 @entity9886 @entity1110 @entity35 @entity250
UNASSIGNED: @entity251 has been added to hormone replacement therapy to treat @entity250 in postmenopausal @entity1 . Whereas @entity172 has been associated with vascular protection, the vascular effects of @entity251 are contradictory and the effects of its association with @entity172 are largely unknown. In this study we determined the effects of @entity251 associated with conjugated @entity1641 @entity172 (CEE) on vascular function using a model of @entity101 postmenopausal female: ovariectomized spontaneously @entity101 @entity35 . Female spontaneously @entity101 @entity35 were divided into sham-operated, ovariectomized (OVX), and OVX treated for 15 days with either CEE alone (OVX+CEE) or associated with @entity251 (OVX+CEE+T). @entity1808 ( @entity1808 )-induced contraction was markedly increased in aortic rings from OVX compared with sham-operated @entity35 . CEE treatment restored @entity1808 responses, a beneficial effect abrogated with CEE+T. CEE treatment also increased endothelium-dependent relaxation, which was impaired in OVX @entity35 . This effect was lost by CEE+T. Treatment of aortas with @entity9886 ( @entity1808 type-1 receptor antagonist) or @entity12824 ( @entity1110 -oxidase inhibitor) restored the endothelium-dependent relaxation in OVX and CEE+T, establishing an interplay between @entity1808 and @entity409 in OVX and CEE+T. The benefits by CEE were associated with downregulation of @entity1110 -oxidase subunits mRNA expression and decreased reactive @entity26 species generation. The association of @entity251 with CEE impairs the benefits of @entity172 on OVX-associated @entity409 and reactive @entity26 species generation in @entity35 aorta by a mechanism that involves phosphorylation of the cytosolic @entity1110 -oxidase subunit @entity11934 (phox).
