Insulin-like growth factor I is involved in XXXX linked angiogenic processes after microembolisation in porcine heart.

@entity650 @entity32 @entity296 @entity39 @entity1356 @entity442 @entity5100 @entity1192 @entity432 @entity3646
OBJECTIVE: Angiogenesis in the porcine heart can be induced by @entity296 following @entity432 . This process is characterised by increased numbers of monocytes/macrophages, known to be potent producers of various mitogens such as insulin-like growth factors (IGF) and interleukins (IL). The aim of the study was to examine gene expression of these factors by means of northern blot hybridisation, slot blot analysis, and in situ hybridisation in a porcine model of coronary angiogenesis. METHODS: Experimental ischaemia and subsequent focal @entity39 were induced by selective injection of 25 microns microspheres into the left circumflex artery. The hearts were excised after 3-168 h of microembolisation, and tissue was collected from a non-ischaemic control area and the circumflex region of the same heart for further analysis. RESULTS: @entity442 was constitutively transcribed in normal porcine myocardium mainly by myocytes. Following microembolisation, @entity442 mRNA expression was significantly increased in the experimental region (1.8-fold) after 72 h and to a lesser extent after 168 h. In the ischaemic region, characterised by capillary sprouting, numerous mononuclear cells contained @entity442 mRNA. In contrast, @entity5100 mRNA levels, constitutively produced by porcine myocytes, were not altered by microembolisation. @entity1192 , @entity1356 , and @entity3646 mRNA expression was undetectable in our animal model, whereas @entity650 was constitutively transcribed in normal and ischaemic heart and remained insensitive to microembolisation and focal @entity39 . CONCLUSION: After microembolisation, increased @entity442 mRNA expression occurred by infiltrating monocytes in areas of microsphere induced focal @entity39 , where capillary sprouting can be detected, suggesting that @entity442 is involved in @entity32 linked angiogenic processes.
