b-Catenin inactivation is a pre-requisite for XXXX retina regeneration.

@entity11444 @entity9950 @entity1573 @entity3067 @entity989 @entity9949
In the present study we explored the role of b-catenin in mediating @entity1573 retina regeneration. The @entity1573 can regenerate its retina by activating stem/progenitor cells present in the @entity3067 ( @entity3067 ) of the eye or via transdifferentiation of the @entity989 ( @entity989 ). Both modes require @entity9950 ( @entity9950 ). We observed, by immunohistochemistry, dynamic changes of nuclear b-catenin in the @entity3067 and @entity989 after injury (retinectomy). b-Catenin nuclear accumulation was transiently lost in cells of the @entity3067 in response to injury alone, while the loss of nuclear b-catenin was maintained as long as @entity9950 was present. However, nuclear b-catenin positive cells remained in the @entity989 in response to injury and were BrdU-/p27+, suggesting that nuclear b-catenin prevents those cells from entering the cell cycle. If @entity9950 is present, the @entity989 undergoes dedifferentiation and proliferation concomitant with loss of nuclear b-catenin. Moreover, retinectomy followed by disruption of active b-catenin by using a signaling inhibitor ( @entity9949 ) or over-expressing a dominant negative form of @entity11444 induces regeneration from both the @entity3067 and @entity989 in the absence of @entity9950 . Our results imply that b-catenin protects cells of the @entity3067 and @entity989 from entering the cell cycle in the developing eye, and specifically for the @entity989 during injury. Thus inactivation of b-catenin is a pre-requisite for @entity1573 retina regeneration.
