Aminoglycoside-induced alterations of XXXX metabolism.

@entity801 @entity7425 @entity1078 @entity95 @entity4509 @entity18685 @entity581 @entity5154 @entity1076 @entity141 @entity4357 @entity855
There exists a strong interaction between @entity581 and @entity4357 , and these membrane lipids are even considered as the drug receptors. To shed some light on the role of such an interaction in the drug nephrotoxicity, we have investigated the influence of @entity581 on @entity7425 metabolism in kidney proximal tubules where these compounds accumulate. Experiments were carried out by measuring 32P labelling of @entity1076 ( @entity18685 ) and of @entity1078 ( @entity1078 ) after incubation of homogenates of isolated proximal tubules with [gamma-32P] @entity855 . The treatment of @entity95 with @entity5154 , @entity801 and @entity4509 (50, 50 and 300 mg/kg/day, respectively for seven days) promoted a decrease in 32P-PI-P2 and an increase in 32P-PI-P, when compared to the respective values observed in tubules from untreated @entity95 . Under these conditions, the extent of modifications in lipid labelling was similar with the three drugs tested. In in vitro experiments, the exogenous addition of the above @entity581 to the incubation medium containing tubule homogenates from untreated @entity95 also produced, in a dose dependent manner, a decrease in 32P-PI-P2 and an increase in 32P-PI-P. In the in vitro experiments, however, @entity4509 and @entity801 appeared to be less potent than @entity5154 . The results indicated moreover that @entity7425 metabolism was more sensitive to the in vivo (vs. in vitro) action of the drugs. @entity4357 are involved in @entity141 transport and/or mobilization processes, and @entity581 are known to interfere with the @entity141 binding to membranes.(ABSTRACT TRUNCATED AT 250 WORDS)
