MicroRNA-15a induces cell apoptosis and inhibits metastasis by targeting @entity6577 in XXXX .

@entity5 @entity1149 @entity8918 @entity164 @entity6577
UNASSIGNED: MicroRNAs (miRNAs) play a critical role in @entity5 development and progression. Aberrant expression of @entity8918 has recently been reported in several @entity5 , but its role in @entity1149 ( @entity1149 ) still remains obscure. We investigated the effects of @entity8918 on proliferation, apoptosis, and metastasis in A549 cells. Eighteen paired @entity1149 and adjacent non- @entity5 lung tissues were surgically removed and immediately snap frozen until total RNA was extracted and confirmed by two independent pathologists. The targets of @entity8918 were predicted by bioinformatics tools. RNA isolation and quantitative real-time PCR (qRT-PCR), Western blot analysis, cell proliferation assay, cell cycle analysis, cell apoptosis assay, and migration and invasion assays were done. The @entity164 ( @entity164 ) or mutant type (MT) 3'-untranslated region (UTR) vectors were co-transfected with @entity8918 or negative control into A549 cells, and after 24  h of transfection, luciferase activity was measured using the Dual-Glo luciferase assay kit. Statistical analysis was performed using SPSS 13.0 software (SPSS, Chicago, IL, USA). P values of less than 0.05 were considered statistically significant. @entity8918 was significantly downregulated in @entity1149 than in adjacent non-cancerous tissues. @entity8918 overexpression remarkably inhibited cell viability, invasion, and migration and promoted the apoptosis of @entity1149 cells. Additionally, inhibition of @entity8918 expression had the opposite effects on @entity5 progression, while cell cycle remained unaltered. Furthermore, we identified that @entity6577 was a target of @entity8918 and negatively regulated by @entity8918 at the translational level. @entity8918 acts as a @entity5 suppressor in @entity1149 by directly targeting @entity6577 and may serve as a potential diagnostic biomarker and therapeutic target for @entity1149 .
