Induction of anti-thymocyte/T lymphocyte antibodies in XXXX injected with lipopolysaccharides from periodontopathic bacteria.

@entity11281 @entity8209 @entity137 @entity19 @entity95 @entity3841 @entity526 @entity2523
We examined the levels of anti-thymocyte/T lymphocyte autoantibody (ATA) in the serum of @entity19 injected intraperitoneally with lipopolysaccharides (LPS) from periodontopathic bacteria; @entity2523 , @entity3841 , @entity8209 , @entity11281 , and non-oral @entity526 . All of the LPS induced IgM-ATA. Among these, LPS from @entity11281 induced the highest level of IgM-ATA, whereas that of @entity2523 induced the lowest. The peritoneal T lymphocytes of @entity19 injected with LPS were bound by IgM-ATA. Peritoneal B-1 (CD5+B) cells stimulated by each LPS produced much more IgM-ATA than splenic B-2 (CD5-B) cells, suggesting that B-1 cells might be responsible for the production of these antibodies. Serum of @entity19 injected with @entity11281 and @entity8209 LPS showed @entity137 against thymocytes in the presence of @entity95 complements. Binding and @entity137 were confirmed by IgM purified from serum of the @entity19 injected with @entity11281 LPS. Furthermore, serum of @entity19 treated with @entity11281 , @entity8209 or A. actinomycetemcomitans LPS inhibited the proliferation of thymocytes. However, purified IgM from the serum of @entity19 treated with @entity11281 LPS failed to produce the same inhibition. Our results suggest that LPS from certain species of periodontopathic bacteria can induce IgM-ATA in the serum and these antibodies may modulate the local immune network in periodontal tissues.
