[Effects of XXXX bone marrow endothelial cell conditioned medium on the growth of yolk sac hematopoietic stem cells and progenitors].

@entity2304 @entity21929 @entity19 @entity8116 @entity24817 @entity1358 @entity4434
OBJECTIVE: To investigate the effects of @entity19 bone marrow endothelial cell conditioned medium (mBMEC-CM) on the growth of yolk sac hematopoietic progenitors. METHODS: The serum-free mBMEC-CM was obtained from subcultures of @entity19 endothelial cell line derived from bone marrow which was established in our laboratory. The @entity19 yolk sacs were harvested on day 8.5 postcoitus (pc) and incubated with 0.1% collagenase in 10% fetal calf serum at 37 degrees C for 40 minutes. Yolk sac cells were incubated in tissue culture dishes at 37 degrees C for 1 hour. Nonadherent cells were collected for semisolid culture assay of granulocyte-macrophage colony forming unit (CFU-GM) and @entity1358 ( @entity1358 ) after being cultured in DMEM with 10% mBMEC-CM and 10% @entity4434 for 24 hours. The number of @entity8116 was counted at day 7 and day 14 respectively. RESULTS: The growth of @entity8116 was supported by mBMEC-CM with @entity2304 . mBMEC-CM could induce the proliferation and differentiation of yolk sac hematopoietic stem cells and progenitors in liquid culture system. The percentages of @entity8116 compared with the 0 hour control were (119.5 +/- 5.7)% and (130.8 +/- 9.8)% respectively after 24 hours liquid culture (P < 0.05). The expansion effects of mBMEC-CM on @entity8116 were enhanced by compounded with @entity24817 ( @entity24817 ) and @entity21929 (TPO). The percentages of @entity8116 compared with the 0 hour control were (132.0 +/- 6.2)% and (176.9 +/- 12.8)% respectively after 24 hours liquid culture (P < 0.01). CONCLUSION: @entity19 bone marrow endothelial cell conditioned medium could support the growth and proliferation of yolk sac hematopoitic stem cells and progenitors, and this promoting effect was further enhanced by addition of @entity24817 and TPO.
