Energy depletion in the liver and in isolated hepatocytes of XXXX -bearing animals.

@entity1 @entity242 @entity5 @entity2495 @entity880 @entity35 @entity5954 @entity855
@entity5 has a great impact on morbidity and mortality in @entity1 undergoing surgery. Failure to maintain lean tissue against @entity5 -induced @entity2495 results in cumulative weight loss and ultimate failure of the host. Cellular free energy is among the factors that regulates metabolic pathways and may be altered in the @entity5 -bearing state. We studied in-vivo and in-vitro @entity855 levels and metabolic parameters in Fischer @entity35 bearing a @entity5954 -induced @entity242 to elucidate the energy state. Tissue @entity855 was measured in freeze-clamped liver and muscle in 15 @entity5 -bearing @entity35 (TBR) at different @entity5 burdens and their pair-fed controls (CTR) by bioluminescence assay. Plasma metabolites, hormones, and enzymes were determined in the same animals. Liver @entity855 level was lower in TBR with a 5% @entity5 burden: 3.07 +/- 0.56 (SE) nmole/mg tissue vs CTR: 5.33 +/- 0.60 (P < 0.05), 10% TBR: 2.48 +/- 0.54 vs CTR: 4.05 +/- 0.63 (n.s.), and 20% TBR: 1.91 +/- 0.21 vs CTR: 3.86 +/- 0.40 (P < 0.01). Muscle @entity855 was not different between TBR and CTR. This progressive loss of liver @entity855 was associated with decreased plasma insulin level (P < 0.001) and with increased alkaline phosphatase level (P < 0.01) by multiple regression. In vitro, hepatocytes were isolated from 8 TBR and 8 CTR by in-situ liver perfusion with collagenase and the cellular @entity855 was measured before and after 60 min incubation with glucogenic substrates. Hepatocytes from TBR decreased @entity855 by 42% (P < 0.05) in 10 mM @entity880 with higher gluconeogenesis, while control hepatocytes maintained the @entity855 .(ABSTRACT TRUNCATED AT 250 WORDS)
