XXXX stimulates secretion of mucin-like glycoproteins by corneal epithelium in vitro and protects corneal epithelium from desiccation in vivo.

@entity13736 @entity5887 @entity70691 @entity1372 @entity95 @entity2397 @entity6693 @entity3621 @entity936 @entity35 @entity7840 @entity2275
The effect of drugs for @entity3621 and @entity2275 (ecabet @entity1372 , @entity5887 , @entity13736 , and @entity70691 ) on the secretion of mucin-like glycoproteins from @entity35 cornea were investigated in vitro and on a short-term, @entity95 @entity936 model in vivo. For the studies in vitro, cultured @entity35 cornea sections (3 mm diameter) were incubated with radiolabeled @entity2397 , rinsed, and then incubated for 30 min in the presence of one of the drugs. The culture media were reacted with Dolichos biflorus agglutinate (DBA)-lectin, and the radioactivity of @entity7840 -bound mucin-like glycoproteins was measured. A cytotoxicity assay confirmed that mucin-like glycoproteins had not leaked from damaged cells. For studies in vivo, eye drop vehicle or drops containing gefarnate were instilled in the eyes of nine anesthetized @entity95 , and then the eyes were kept open with specula for two hours. These @entity95 and two control @entity95 not subjected to ocular drying were killed, and their eyes were enucleated and stained with @entity6693 . Corneal epithelial damage from desiccation was evaluated based on the extent of @entity6693 staining. Among the four kinds of drugs for @entity3621 and @entity2275 , only gefarnate significantly increased the mucin-like glycoprotein secretion from cultured @entity35 corneas in vitro; this stimulatory effect of gefarnate was dose-dependent. In vivo, the instillation of gefarnate reduced corneal epithelial damage from desiccation in a dose-dependent fashion. These results suggest that gefarnate reduces desiccation of corneal epithelium, perhaps by stimulating secretion of mucin-like glycoproteins from corneal epithelium.
