Macrophages induce @entity3840 expression in XXXX cells: role of @entity1356 autoamplification.

@entity1 @entity7242 @entity0 @entity1356 @entity5 @entity1421 @entity26 @entity1193 @entity1110 @entity11346 @entity20994 @entity3840
@entity5 -associated macrophages and high levels of @entity3840 ( @entity3840 ) are associated with poor prognosis in @entity0 @entity1 , but their potential interdependence has not been evaluated. The objective of this study was to determine whether macrophages regulate @entity3840 expression in @entity0 cells. For this purpose, THP-1 cells were cocultured with @entity1 @entity0 cells. Coculture led to increased @entity3840 expression in the @entity1 cells and elevated @entity1421 levels in conditioned media. Similar results were observed when THP-1 cells were incubated with @entity1 @entity0 cells or when @entity1 monocyte-derived macrophages were cocultured with @entity1 cells. Coculture triggered production of reactive @entity26 species (ROS) in @entity1 cells. @entity3840 induction was blocked in cells preincubated with an reduced @entity1110 ( @entity1110 ) oxidase inhibitor or by silencing @entity20994 , a subunit of @entity1110 oxidase. ROS production triggered activation of @entity7242 and mitogen-activated protein kinases (MAPKs). Blocking @entity7242 or MAPK activities or antagonizing the @entity11346 ( @entity11346 ) transcription factor attenuated @entity3840 induction in @entity1 cells. Coculture caused rapid induction of @entity1356 ( @entity1356 ) in both @entity0 cells and macrophages. Increased @entity1356 expression was blocked by an @entity1193 ( @entity1193 ), suggesting autocrine and paracrine effects. Importantly, macrophage-induced @entity3840 expression was blocked in @entity1 cells preincubated with @entity1193 or anti- @entity1356 IgG. Together, these results indicate that macrophage-mediated induction of @entity3840 in @entity0 cells is a consequence of @entity1356 -mediated stimulation of ROS > @entity7242 > MAPK > @entity11346 signaling. @entity1356 -dependent induction of @entity3840 in @entity0 cells provides a mechanism whereby macrophages contribute to @entity5 progression and potential therapeutic targets in @entity0 .
