Downregulation of @entity1262 , Ras and p- @entity1366 contributes to the @entity4447 -induced reduction in drug resistance towards @entity632 in XXXX cell lines.

@entity632 @entity4447 @entity1262 @entity1 @entity137 @entity9188 @entity5 @entity3105 @entity1366 @entity356
UNASSIGNED: Multidrug resistance (MDR) to @entity632 ( @entity3105 ) limits its effectiveness against @entity5 cells. @entity4447 ( @entity9188 ) has been reported to reduce MDR in various types of @entity5 , but the mechanisms involving Ras and @entity1262 ( @entity1262 ) remain to be fully elucidated. The objectives of the present study were to evaluate @entity9188 in reversing MDR to @entity3105 , and to identify the association in antitumor activities between the effectiveness of @entity3105 and Ras/phosphorylated (p  -) extracellular signal  -regulated kinase (ERK)1/2  signaling in SGC7901/ADM and SGC7901/S @entity1 @entity356 cell lines. @entity137 and sensitivity towards @entity9188 were assessed using non  -toxic and mildly  -toxic concentrations (0.1  and  0.5    M, respectively). The reversing effect of @entity9188 on MDR was investigated prior to and following treatment with a cytokine activation of the recombinant @entity1 granulocyte colony stimulating factor ERK pathway. The SGC7901/ADM and SGC7901/S cells had the same sensitivity to @entity9188 . The SGC7901/ADM cells were resistant to @entity3105 and @entity9188 treatment reduced the level of resistance to @entity3105 (P<0.01). The expression of @entity1262 ( @entity1262 ) in the SGC7901/ADM cells was higher than in the SGC7901/S cells (P<0.001). @entity9188 treatment decreased the levels of P  -gp in a time  - and dose  -dependent manner (P<0.01). The expression of Ras was higher in the SGC7901/ADM cells than in the SGC7901/S cells, while the expression of p  - @entity1366 remained the same. @entity9188 decreased the levels of Ras and p  - @entity1366 (P<0.01). Following pretreatment with rhG  -CSF, the levels of Ras and p  - @entity1366 were further decreased (P<0.01). Drug  -resistant @entity356 cells had higher expression levels of P  -gp and Ras, but not of p  - @entity1366 . Non  - and mildly  -toxic doses of @entity9188 reduced MDR to @entity3105 through Ras/p- @entity1366 signaling.
