[Immunohistochemical evaluation of the percentage of proliferating neoplastic cells of @entity3969 in XXXX by means of the @entity1758 monoclonal antibody].

@entity1 @entity5 @entity982 @entity1758 @entity3969
Quantifying the proliferation of neoplastic cells is an important and objective element in estimating the degree indicating the @entity5 and their reaction to applied treatment. The basic problem in the procedure dealing with the @entity3969 in @entity1 is the classification of @entity1 into appropriate risk groups. The goal of this study was to explore the possibility of determining the proliferation index in @entity3969 with the aid of monoclonal antibody @entity1758 . The material comprised segments of the @entity5 taken from 35 @entity1 treated at the @entity1 and Youth Oncology Clinic of the Mother and @entity1 Institute in Warsaw as well as at the Pediatric Surgery Clinic of the Pomeranian Medical Academy in Szczecin in the years from 1982 to 1994. Specimens for immunohistochemical investigations were prepared from the tissues of @entity5 , fixed in @entity982 and embedded in paraffin blocs. The positive response of the reaction for the Ki-67 antigen was visible in the form of red-stained granules (Figs. 1, 2, 3, 4). The index for proliferation of the cell nuclei was determined in the individual components of the @entity5 : blastema, epithelial tissue and stroma. Distinct difference was disclosed to exist between low values for stromal cells (3.3%) and high ones for blastemic cells (22.3%) and epithelial cells (23.7%). The post chemotherapy group revealed higher values of the index within the epithelial cells and lower among blastemic cells as compared to the group without chemotherapy. The prognostic significance of the proliferation index when using monoclonal antibody @entity1758 was studied by dividing the @entity5 into two groups, namely: below and above 20% of the index value. It was noticed that there was a concurrency of higher proliferation values among blastemic and epithelial cells with worse prognosticating cases. The cases, in which after chemotherapy the index of proliferation within blastema persisted being high, were recorded to predict poorer prognosis.
