[Long-term maintenance of XXXX @entity1085 as xenografts following explant organ culture].

@entity1 @entity1085 @entity9975 @entity19
The purpose of present study is to establish a method that should enable us to keep a @entity1 gall-bladder epithelial tissue alive for a period long enough to observe morphological change after an exposure to a certain carcinogenic agents. @entity1 @entity1085 obtained from surgically resected specimen were kept as an explant organ culture in vitro for the first 2 weeks. They were, then, xenotransplanted to the subcutaneous (S.C.) space of the athymic @entity19 . The gall-bladder tissues obtained from 7 cases of @entity9975 and 1 case of cholecysto-choledocholithiasis were used as materials in the present study. And the longest survivor among them in the @entity19 was extensively studied in the present paper. After an interval of 4 to 27 weeks, the xenografts were recovered from the recipient @entity19 and studied by light microscopy and histochemistry. The morphological changes of the explant epithelia during the organ culture were also studied. Parts of the explants were implanted in 6 @entity19 . Each of the @entity19 received 3 pieces of explants on the s.c. space in both of their flanks. All xenografts showed subcutaneous cyst formation. The cyst obtained 4 weeks after the implantation was consisted of a monolayer of cuboidal epithelial cells and those recovered from the recipient @entity19 24 and 27 weeks after the implantation were consisted of well growing columnar and cuboidal epithelial cells. In addition, sinus-like structures were observed beneath the cystic epithelia. The viability of the cells was excellent.(ABSTRACT TRUNCATED AT 250 WORDS)
