PLGA microspheres for XXXX treatment: preliminary "in vitro"/"in vivo" evaluation.

@entity32 @entity604 @entity170 @entity7050 @entity2331 @entity155 @entity35 @entity4260
The aim of this work was to prepare and to evaluate "in vitro"/"in vivo" microspheres based on @entity7050 copolymers containing @entity4260 , for the local treatment of oral @entity604 . The first objective was the preparation and "in vitro" characterization of @entity4260 loaded microspheres, by emulsion/solvent evaporation method. Process parameters such as drug:polymer weight ratio, and molecular weight of copolymers, were also investigated. The second objective was to elaborate a suitable animal model of @entity170 , to evaluate the efficacy of these microparticulate drug delivery systems. "In vivo" experiments were carried out on female @entity35 , in which @entity2331 was induced by gonadectomy and molar @entity155 . Morphometric analysis of mandibular segment were carried out to quantify the development of @entity2331 and the efficacy of drug loaded microspheres. Results showed that @entity4260 loaded PLGA microspheres can be successfully obtained with good "in vitro" characteristics, utilizing the emulsification/solvent evaporation method. "In vivo" experiments revealed that local administration of microspheres produced only mild @entity32 on the injection site. Morphometric analyses showed, at the level of the third molar, a slight increase in spongy and total bone mass on @entity35 jaw treated with microspheres with respect to control. Control animals exhibited a scarce degree of @entity2331 demonstrating that this animal model is not suitable for this purpose.
