Affect of antidepressants on the in vitro differentiation of XXXX bone marrow mesenchymal stem cells into neuronal cells.

@entity905 @entity13707 @entity5829 @entity14919 @entity72166 @entity2343 @entity2645 @entity35 @entity38224
BACKGROUND: Bone marrow is a valuable source of mesenchymal stem cells (MSCs) that can be used in regenerative medicine. MSCs are able to differentiate into cells from all three germ layers under specific conditions. The aim of the current work was to study the differentiation of @entity35 MSCs (rMSCs) into neuron-like cells. NEW METHOD: We investigated how the antidepressants @entity2645 , @entity5829 , @entity905 and @entity13707 affect the differentiation of rMSCs. Furthermore, we present differentiation cocktails using a cortex astrocyte-conditioned medium (CACM) separately or in conjunction with each of the antidepressants and investigated their additive effect on the efficiency of differentiation. We also observed how various differentiation conditions affect the number of primary @entity2343 per cell. RESULTS: Gene expression for an early neuronal marker (b-III-tubulin) and markers that are typical for adult neurons such as Th, @entity14919 and @entity38224 were observed. The @entity72166 and @entity14919 gene expression were up-regulated, Th decreased slightly and @entity38224 was down-regulated after differentiation We observed a two-fold higher percentage of b-III-tubulin positive cells after treatment with antidepressants and two-fold increase of neuron-like cells after using CACM with @entity2645 or @entity905 simultaneously. Differentiation using @entity2645 or in conjunction with CACM and @entity5829 or @entity905 simultaneously increased the number of cell dendrites. COMPARISON WITH EXISTING METHODS: The results that were obtained are completely new and need further investigations in the nearest future. CONCLUSIONS: These results suggest that antidepressants improve differentiation efficiency of rMSCs and may be useful in the preparation of rMSCs for transplantation. Differentiation efficiency is higher after long-term exposure to antidepressants, than after a 24-h exposure. Nearly additive effect of CACM and @entity2645 or @entity905 suggests a beneficial role of antidepressants after transplantation.
