[Time-related expression of adhesive proteins and other markers of age of XXXX ].

@entity1 @entity32 @entity4725 @entity19 @entity4105 @entity1196 @entity8729 @entity272 @entity2071 @entity130
In addition to evidence of the vital reaction it is important from the forensic aspect to assess the age of wounds, in particular in wounds with a short time of survival. To both can contribute detection of adhesive molecules, identified in recent years, possibly in combination with other markers of early stages of reparative @entity32 . The submitted paper comprises the results of investigation of 465 skin wounds. The investigated samples were obtained from necroptic material, excision from wounds of treated @entity1 and from experimental @entity130 in @entity19 . Assessment of the age of @entity130 by means of endothelial adhesive molecules was made in paraffin sections after transfer into Varioclave and using the ABC techniqueref.. In @entity1 material a strong positive reaction of @entity2071 was observed first after 1 and a half hours and latest after 3 and a half days, in @entity8729 first after 3 mins., latest after 7 hours, in @entity4105 first after 1 hour and latest after 17 days, in @entity4725 first after 3 hours and latest 3 and a half days after the development of the injury. Expression of @entity1196 was not typical for the injury. In @entity272 of @entity19 positive immunohistochemical reactions were found as a rule sooner than in @entity272 of @entity1 . Fibronectin was detected in paraffin sections from 70 skin wounds of dissected subjects immunohistochemically by the indirect immunoperoxidase reaction and by the use of the ABC technique labelled with alkaline phosphatase. Positive evidence was observed first after 5 minutes and latest after 8 hours. CONCLUSION: Detection of a rise of expression of @entity2071 , @entity4725 and P- and E. selectin and the formation of basic netlike fibronectin structures improves the assessment of the age of wounds with a short survival period.
