In vivo sensitization to purified XXXX proteins in @entity283 sensitized to natural rubber latex.

@entity1 @entity283 @entity566 @entity1097 @entity15543 @entity4973
BACKGROUND: Thirteen proteins of natural rubber latex ( @entity15543 ) known to bind @entity1 IgE have been isolated and characterized as @entity4973 b allergens. However, the in vivo importance of native @entity4973 b allergens has not been defined in @entity283 ( @entity283 ) with natural rubber latex (NRL) @entity566 . OBJECTIVES: The principal aim of this study was to identify the major in vivo @entity4973 b allergens in @entity283 with NRL @entity566 confirmed by percutaneous sensitivity to nonammoniated latex (NAL). METHODS: Skin prick testing was performed with 7 (native) proteins purified from NAL ( @entity4973 b 1, 2, 3, 4, 6.01, 7.01, and a newly described @entity4973 b 13) and recombinant @entity4973 b 5 in 62 @entity283 with histories of NRL @entity566 (group 1) confirmed by percutaneous reactivity to NAL and in 49 @entity1097 without NRL @entity566 (group 2). Serial 10-fold concentrations of @entity4973 b proteins (5 x 10(-5) microg/mL to 50 microg/mL) were tested; serum samples of subjects were assayed for serum specific IgE by immunoassays. RESULTS: @entity4973 b 2, @entity4973 b 5, @entity4973 b 6.01, and @entity4973 b 13 produced skin reactions in more than 60% of group 1 subjects, with @entity4973 b 1, 3, 4, and 7.01 eliciting reactions in less than 50%. Only 1 of 49 group 2 workers reacted to a single Hev b antigen ( @entity4973 b 13). Specificity of 7 @entity4973 b allergens was 100% and 98% for @entity4973 b 13 in identifying workers with confirmed NRL @entity566 . Specific IgE by AlaSTAT and CAP immunoassays was elevated in 40 of 60 (67%) and 33 of 62 (53%) of NAL-reactive workers and produced false-positive test results in 4 of 49 (8%) and 3 of 48 (6%) group 2 subjects, respectively. CONCLUSION: @entity4973 b 2, 5, and 6.01 are major in vivo allergens and @entity4973 b 13 is a new major in vivo allergen among @entity283 with @entity566 to NRL.
