Neuronal-binding antibodies from patients with XXXX induce cognitive deficits following intrathecal passive transfer.

@entity1 @entity19 @entity1701 @entity146
Antiphospholipid antibodies (aPL) have been suggested to play a role in causing cognitive and @entity146 . In the present study we investigated the pathogenic potential of aPL by intracerebro-ventricular (ICV) administration of immunoglobulins (IgG) from patients with @entity1701 ( @entity1701 ). IgG, purified from the sera of four @entity1701 @entity1 , was tested for binding to normal @entity19 brain by immunohistological staining. These IgG (7.5 microg) were injected ICV unilaterally to male C3H @entity19 . @entity19 injected with IgG purified from pooled sera derived from healthy subjects served as controls. The @entity19 were examined neurologically for motor function and coordination, and cognitively in a Morris water maze. The cognitive tests were performed with the experimenter blinded to the treatment. The performance of the @entity19 in four separate experiments was compared by analysis of variance with repeated measures. IgG from one @entity1701 @entity1 was found to bind best to neuronal structures in the hippocampus and cerebral cortex. @entity19 (n = 43) injected with this IgG performed worse in the water maze compared to the controls (n = 45) with significant effects of the aPL IgG on the overall performance of the @entity19 (treatment, P < 0.03), on learning throughout the experiment (treatment x day, P < 0.02) and on short term memory (treatment x day xtrial, P < 0.002). IgG injected from two of the three other @entity1 also bound specifically to @entity19 brain neurons and produced an impairment in performance of the water maze. These results support the hypothesis that aPL that gain access to the central nervous system may play a direct role in the pathogenesis of neurological manifestations of @entity1701 .
