Involvement of a putative response regulator Brrg-1 in the regulation of sporulation, sensitivity to fungicides, and osmotic stress in XXXX .

@entity274 @entity19621 @entity18389 @entity1219 @entity20346 @entity1980 @entity14261 @entity36096 @entity4338
The response regulator protein is a core element of two-component signaling pathway. In this study, we investigated functions of BRRG-1 of @entity14261 , a gene that encodes a putative response regulator protein, which is homologous to Rrg-1 in @entity1980 . The BRRG-1 gene deletion mutant /\Brrg1-62 was unable to produce conidia. The mutant showed increased sensitivity to osmotic stress mediated by @entity1219 and @entity4338 , and to oxidative stress generated by @entity274 . Additionally, the mutant was more sensitive to the fungicides @entity36096 , @entity18389 , and @entity20346 than the parental strain. Western-blot analysis showed that the Bos-2 protein, the putative downstream component of Brrg-1, was not phosphorylated in the /\Brrg1-62. Real-time polymerase chain reaction assays showed that expression of @entity19621 also decreased significantly in the mutant. All of the defects were restored by genetic complementation of the /\Brrg1-62 with the wild-type BRRG-1 gene. Plant inoculation tests showed that the mutant did not show changes in pathogenicity on rapeseed leaves. These results indicated that Brrg-1 is involved in the regulation of asexual development, sensitivity to @entity36096 , @entity18389 , and @entity20346 fungicides, and adaptation to osmotic and oxidative stresses in @entity14261 .
