Prognostic significance of flow XXXX . A 5-year study.

@entity1 @entity4153 @entity894 @entity5 @entity2 @entity189 @entity2615 @entity152 @entity420
Flow cytometrically determined cellular DNA content has been measured on specimens from 101 @entity1 affected by @entity2 (40 epidermoid cell @entity189 , 22 @entity420 , 21 @entity152 , 11 @entity4153 , and seven @entity189 ), and one by @entity2615 . Ninety-eight of 102 (96%) @entity1 with @entity5 evidenced the occurrence of at least one cytometrically aneuploid cell subpopulation. Fifty-five of 102 (54%) cases evidenced the occurrence of multiclonality, that is, the presence of more than one aneuploid stem cell line. However, the incidence of @entity2 was statistically different in surgical cases (where multiple site sampling from the primary and lymph nodes was possible) in comparison to the nonsurgical ones (e.g., bronchial washing): 48/77 (62%) and six of 24 (25%), respectively. Therefore, only the 77 surgical @entity1 were used for further analysis. The cases were classified according to the @entity894 ( @entity894 ) in the following way: Group A ( @entity5 with one or more stem lines with @entity894 ranging from 1 to 2) and Group B ( @entity5 with at least one stem line with @entity894 less than 1 or greater than 2). A significant correlation has been found between the cytometric ploidy condition so defined (Groups A and B) and the @entity5 mass doubling time (DT), Group B being associated with fast growing @entity5 (DT lower than 90 days). A statistically better 12-month survival rate (5-year maximum follow-up) was observed in Group A (88%) in respect to Group B (47%) and is evident in the @entity1 survival time course. A better prognostic indication can be achieved by stratifying the @entity1 according to both the cytometric ploidy condition and the @entity5 DT. Flow cytometric data can usefully contribute to the prognostic @entity2 provided that representative cellular material is collected by multiple site sampling.
