A bow-tie genetic architecture for morphogenesis suggested by a genome-wide RNAi screen in XXXX .

@entity72595 @entity2199 @entity61447 @entity72592 @entity72590 @entity72596 @entity72594 @entity43474 @entity72597 @entity30794 @entity72591 @entity72593 @entity5672
During animal development, cellular morphogenesis plays a fundamental role in determining the shape and function of tissues and organs. Identifying the components that regulate and drive morphogenesis is thus a major goal of developmental biology. The four-celled tip of the @entity2199 male tail is a simple but powerful model for studying the mechanism of morphogenesis and its spatiotemporal regulation. Here, through a genome-wide post-embryonic RNAi-feeding screen, we identified 212 components that regulate or participate in male tail tip morphogenesis. We constructed a working hypothesis for a gene regulatory network of tail tip morphogenesis. We found regulatory roles for the posterior Hox genes @entity72590 , the TGF-b pathway, nuclear hormone receptors (e.g. @entity61447 ), the heterochronic gene @entity72591 , and the GATA transcription factors @entity72592 and @entity72593 . The majority of the pathways converge at @entity72594 and @entity72595 . In addition, @entity61447 and @entity72594 / @entity72595 regulate each others' expression, thus placing these three genes at the center of a complex regulatory network. We also show that @entity72594 and @entity72595 negatively regulate other signaling pathways and affect downstream cellular processes such as vesicular trafficking (e.g. @entity72596 , @entity72597 ) and rearrangement of the cytoskeleton (e.g. @entity43474 , @entity30794 , and @entity5672 ). Based on these data, we suggest that male tail tip morphogenesis is governed by a gene regulatory network with a bow-tie architecture.
