Mucosal Alpha-Papillomaviruses are not associated with XXXX : Lack of Mechanistic Evidence from South Africa, China and Iran and from a World-Wide Meta-Analysis.

@entity1 @entity986 @entity5 @entity406 @entity1721 @entity72251 @entity1799 @entity285
UNASSIGNED: Epidemiological and mechanistic evidence on the causative role of @entity1 papillomaviruses (HPV) in @entity1721 ( @entity1721 ) is unclear. We retrieved @entity285 - and @entity406 -fixed paraffin-embedded @entity1721 tissues from 133 @entity1 seropositive for antibodies against @entity72251 , from high-incidence @entity1721 regions; South Africa, China, and Iran. With rigorous care to prevent nucleic acid contamination, we analyzed these tissues for the presence of 51 mucosotropic @entity1 alpha-papillomaviruses by two sensitive, broad-spectrum genotyping methods, and for the markers of HPV-transformed phenotype: (i) @entity1799 /18 viral loads by quantitative real-time PCR, (ii) type-specific viral mRNA by E6*I/E6 full-length RT-PCR assays and (iii) expression of cellular protein @entity986 ( @entity986 ) . Of 118 analyzable @entity1721 tissues, 10 (8%) were positive for DNA of HPV types: 16 (four @entity5 ); 33, 35, 45 (one @entity5 each); 11 (two @entity5 ); and 16, 70 double infection (one @entity5 ). Inconsistent HPV DNA+ findings by two genotyping methods and negativity in qPCR indicated very low viral loads. A single @entity1799 DNA+ @entity5 additionally harbored @entity1799 E6*I mRNA but was @entity986 ( @entity986 ) negative ( @entity1799 E1 seropositive @entity1 ). Another @entity1799 DNA+ @entity5 from an @entity1799 E6 seropositive @entity1 showed @entity986 ( @entity986 ) up-regulation but no @entity1799 mRNA. In the @entity5 tissues of these serologically preselected @entity1721 @entity1 , we did not find consistent presence of HPV DNA, HPV mRNA or @entity986 ( @entity986 ) up-regulation. These results were supported by a meta-analysis of 14 other similar studies regarding HPV-transformation of @entity1721 . Our study does not support the etiological role of the 51 analyzed mucosotropic HPV types in the @entity1721 . This article is protected by copyright. All rights reserved.
