Comparative Characterization of XXXX O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques.

@entity1 @entity3081 @entity2756 @entity1838 @entity2177
UNASSIGNED: We used standardized methodologies to characterize @entity1838 O1 isolates from Guinea, Democratic Republic of the Congo (DRC), Togo, C  te d'Ivoire and Mozambique. We investigated 257 @entity1 isolates collected in 2010 to 2013. DRC isolates serotyped O1 Inaba, while isolates from other countries serotyped O1 Ogawa. All isolates were biotype El Tor and positive for @entity3081 toxin. All isolates showed multidrug resistance but lacked @entity2177 resistance. Antimicrobial susceptibility profiles of isolates varied between countries. In particular, the susceptibility profile of isolates from Mozambique (East-Africa) included resistance to @entity2756 and was distinctly different to the susceptibility profiles of isolates from countries located in West- and Central-Africa. Molecular subtyping of isolates using pulsed-field gel electrophoresis (PFGE) analysis showed a complex relationship among isolates. Some PFGE patterns were unique to particular countries and clustered by country; while other PFGE patterns were shared by isolates from multiple countries, indicating that the same genetic lineage is present in multiple countries. Our data add to a better understanding of @entity3081 epidemiology in Africa.
