Expression and localization of inducible XXXX synthase in anti- @entity5399 @entity631 .

@entity9554 @entity66 @entity631 @entity5399 @entity842 @entity3623 @entity7622
To elucidate a possible involvement of @entity842 in the development of a mesangial proliferative @entity631 induced by anti- @entity5399 antibody administration, glomerular expression of three isoforms of @entity3623 ( @entity3623 ), @entity3623 ( @entity3623 ), brain @entity3623 , and endothelial @entity3623 , was examined at both mRNA and protein levels by ribonuclease protection assay and immunofluorescence microscopy. Light microscopy showed an accumulation of polymorphonuclear leukocytes at 1 hour, lysis of mesangial cells at 1 day, a @entity7622 at 4 to 10 days, and minimal residual glomerular lesions by 28 days. Ribonuclease protection assay showed that the glomerular expression of @entity3623 mRNA peaked at 1 hour and decreased thereafter. No substantial expression of @entity3623 mRNA was observed in normal glomeruli or in the nephritic glomeruli obtained at different time points (1, 4, 10, or 28 days). By immunofluorescence microscopy with a specific monoclonal antibody, an intense reaction for @entity3623 was demonstrated in a few cells in the glomeruli at 1 hour. Most of the @entity3623 -positive cells were identified as polymorphonuclear leukocytes. @entity3623 -positive cells were found less frequently in the glomeruli on days 1 and 4. @entity9554 mRNA was constitutively expressed in normal glomeruli and increased biphasically with two peaks at 1 hour and at 4 days or later; however, the peak expression was much less than that of @entity3623 mRNA at 1 hour. Expression of brain @entity3623 mRNA was not detectable in either normal or nephritic glomeruli. These results show that @entity3623 is predominantly expressed in polymorphonuclear leukocytes accumulating at 1 hour in the glomeruli of anti- @entity5399 @entity631 and suggest an involvement of NO in the @entity66 .
