Early intracellular events induced by in vivo leptin treatment in XXXX skeletal muscle.

@entity16404 @entity3115 @entity6913 @entity13437 @entity19 @entity10393 @entity10433 @entity3241 @entity14881 @entity13436 @entity1994 @entity669 @entity1995 @entity3238
Experimental evidence suggests that leptin may exert direct effects on peripheral tissues. In this study we investigated some transductional molecules in skeletal muscle, after intraperitoneal leptin injection in wild-type and ob/ob @entity19 . By immunoprecipitation and immunoblotting with anti- @entity10393 antibodies, we observed a modified pattern of @entity10393 proteins. We then identified an increase in @entity16404 , @entity3241 and @entity3238 @entity669 -phosphorylation and in their association with @entity14881 , a subunit of @entity3115 . The increase in @entity3115 activity in immunoprecipitated @entity14881 did not reach statistical significance, however, both @entity1994 and @entity6913 resulted significantly hyper-phosphorylated. Bad, an @entity1994 substrate involved in cell survival, appeared modified in its phosphorylation. @entity13437 , @entity13436 and @entity1995 MAP kinase phosphorylation significantly increased, even if the latter only in wild-type animals. Finally, by EMSA experiments, we documented that leptin increased the DNA binding capacity of @entity10433 homodimers and AP-1. Thus, leptin appears to activate, within minutes, some insulin signalling molecules. @entity10433 and AP-1 activation by gene expression remodelling could subsequently trigger more leptin-specific effects. Further, leptin might play a still underestimated role in cell survival.
