Metabolic Interaction of the Active Constituents of Coptis chinensis in XXXX Liver Microsomes.

@entity1 @entity66040 @entity7652 @entity34683 @entity12598
Coptis chinensis is commonly used in traditional Chinese medicine. The study investigated metabolic interaction of the active constituents ( @entity7652 , @entity34683 , @entity12598 , and @entity66040 ) of Coptis chinensis in @entity1 liver microsomes. After incubation of the four constituents of Coptis chinensis in HLMs, the metabolism of the four constituents was observed by HPLC. The in vitro inhibition experiment between the active constituents was conducted, and IC50 value was estimated. @entity34683 exhibited inhibitions against the formation of the two metabolites of @entity7652 with IC50 values of 6.5 and 8.3    M, respectively. @entity12598 and @entity66040 showed the weaker inhibitory effect on the formation of the metabolites of @entity7652 . @entity7652 showed a weak inhibitory effect on the production of @entity34683 metabolite with an IC50 value of 115    M, and @entity12598 and @entity66040 had little inhibitory effect on the formation of @entity34683 metabolite. @entity7652 , @entity34683 , and @entity66040 showed no inhibitory effect on the generation of @entity12598 metabolite (IC50 > 200    M). The findings suggested that there are different degrees of metabolic interaction between the four components. @entity34683 showed the strongest inhibition toward @entity7652 metabolism.
