XXXX attenuates lipopolysaccharide-induced @entity8 .

@entity1 @entity32 @entity8 @entity19 @entity740 @entity6108 @entity2842 @entity1199 @entity687
@entity8 is the main pathological change in many @entity740 . @entity6108 , a yellow pigment present in the rhizome of turmeric (Curcuma longa L. Zingiberaceae), was found to be a potential anti-inflammatory agent. The present study aimed to investigate the effects of @entity6108 on the @entity32 of @entity19 kidney and cultured renal tubular epithelial cells ( @entity1 cells) induced by lipopolysaccharide (LPS) and to explore the mechanism. @entity6108 was injected intraperitoneally before LPS administration. @entity8 was assessed by evaluating @entity2842 ( @entity2842 ) expression and macrophage infiltration in renal tissue using immunohistochemical methods, and also by measuring renal @entity2842 mRNA level using Real-Time polymerase chain reaction (PCR). @entity1 cells were cultured to investigate the in vitro effect of @entity6108 against @entity8 . The expression of @entity2842 and @entity687 ( @entity687 ) mRNA was measured by Real-Time PCR. The expression of @entity2842 and @entity687 protein in supernatant was detected by enzyme-linked immunosorbent assay (ELISA). The activity of nuclear factor (NF)-kB was detected by electrophoretic mobility shift assay (EMSA). The results demonstrated that @entity6108 could inhibit LPS-induced renal @entity2842 mRNA expression. @entity6108 also significantly inhibited the expression of @entity2842 and @entity1199 mRNA in @entity1 cells, and partially inhibited the secretion of @entity2842 and @entity687 . Furthermore, @entity6108 was found to inhibit the DNA-binding activity of NF-kB. The present study demonstrated that @entity6108 has a protective effect on LPS-induced @entity8 , and this effect might be attributed to its inhibitory effects on @entity2842 mRNA expression and DNA-binding activity of NF-kB. Hence, @entity6108 might be potentially useful in some @entity8 by preventing @entity8 .
