Novel proteins linking the actin cytoskeleton to the endocytic machinery in XXXX .

@entity71011 @entity375 @entity20008 @entity71010
The importance of coupling the process of endocytosis to factors regulating actin dynamics has been clearly demonstrated in @entity375 , and many proteins involved in these mechanisms have been identified and characterized. Here we demonstrate the importance of two additional cortical components, @entity71010 and @entity71011 , which together are essential for the organization of the actin cytoskeleton and for fluid phase endocytosis. Both @entity71010 and @entity71011 were identified through two-hybrid screens with different domains of the adaptor protein @entity20008 . @entity71010 colocalizes with cortical actin and requires the presence of an intact actin cytoskeleton for its cortical localization. Ycl034w/ @entity71011 localizes to the cell cortex but does not colocalize with actin. The @entity71011 protein contains putative VHS and GAT domains as well as an NPF motif, which are all domains characteristic of proteins involved in membrane trafficking. Deletion of either gene alone does not confer any dramatic phenotype on cells. However, deletion of both genes is lethal at elevated temperatures. Furthermore, at all temperatures this double mutant has depolarized actin and an almost undetectable level of fluid phase endocytosis. Our data demonstrate that @entity71010 and @entity71011 are important components of complexes involved in overlapping pathways coupling endocytosis with the actin cytoskeleton in @entity375 .
