Immunohistochemical evaluation of venous leg ulcers before and after negative pres XXXX y.

@entity1 @entity4 @entity1066 @entity469 @entity3179 @entity2279 @entity5173 @entity2636
Ven @entity3179 present a medical challenge. Whenever possible, therapy should be causal and include compression therapy and surgery. Negative pressure wound therapy (NPWT) has been successfully used in several phases of venous leg ulc @entity1066 eatment. Positive effects of NPWT, such as reduction of ede @entity4 ra @entity5173 wound exudate, and ac- celeration of granulation tissue formation, are reasons for recommending NPWT in order to improve healing rates. AIM: The main goal of this study was to evaluate, using immunohistochemical markers, the efficacy of NPWT in terms of neoangiogenesis and granulation tissue promotion in the treatment of hard-to-heal ven @entity3179 ETHODS: Thirty pat @entity1 th hard-to-heal ven @entity3179 re included. The pat @entity1 re divided into two groups: one group treated with NPWT, pol @entity2636 am, and four-layer bandaging system, and the second group with moist wound dressings and four-layer bandaging system. Pat @entity1 re moni- tored before and after 1 week of treatment with multiple biopsies taken from the wound bed and wound edge. Immunohistochemical evaluation included markers for angiogenesis (CD3 @entity2279 lymphatic vessels (D240), macrophages (CD6 @entity469 and lymphocytes (CD3). RESULTS: All pat @entity1 - cluded in the NPWT group, after 1 week, showed a significant improve- ment in terms of angiogenesis, lymphatic vessels, and macrophage and lymphocyte proliferation, compared to the control group. CONCLUSION: This study objectively demonstrated the efficacy of NPWT in hard-to- heal ven @entity3179 a immunohistochemical findings. In particular, the results showed rapid granulation and neoangiogenesis promotion. In the authors' opinion, NPWT must be included as an adjuvant to standard venous leg ulc @entity1066 erapy.
