Dual RNA-seq reveals Meloidogyne graminicola transcriptome and candidate effectors during the interaction with XXXX plants.

@entity2199 @entity462 @entity30130 @entity37918 @entity71729 @entity4801 @entity579
UNASSIGNED: Root-knot @entity2199 secrete proteinaceous effectors into plant tissues to facilitate infection by preventing host defenses and reprogramming the host metabolism to their benefit. Meloidogyne graminicola is a major pest of @entity579 ( @entity579 ) in Asia and in Latin America, causing important crop losses. The goal of this study was to identify @entity37918 pathogenicity genes expressed during the plant- @entity2199 interaction. Using the dual RNA-seq strategy, we generated transcriptomic data of @entity37918 samples covering the pre-parasitic J2 stage and 5 parasitic stages in @entity579 plants, from the parasitic J2 to the adult female. In the absence of a reference genome, a de novo @entity37918 transcriptome of 66 396 contigs was obtained from those reads that were not mapped on the @entity579 genome. Gene expression profiling across the @entity37918 life cycle revealed key genes involved in the @entity2199 development and provided insights into genes putatively associated with @entity4801 . Development of a "secreted protein prediction" pipeline revealed a typical set of proteins secreted by @entity2199 , as well as a high number of @entity462 -rich proteins and putative nuclear proteins. Combined to expression data, this pipeline enabled identification of 15 putative effector genes, including two homologs of well-characterized effectors from cyst @entity2199 ( @entity30130 -like and @entity71729 ), and a metallothionein. The localization of gene expression was assessed by in situ hybridization for a subset of candidates. All these data represent important molecular resources to elucidate @entity37918 biology and to select for potential targets to develop novel control strategies for this @entity2199 species. This article is protected by copyright. All rights reserved.
