MicroRNA-196a overexpression promotes cell proliferation and inhibits cell apoptosis through XXXX / @entity584 /FOXO1 pathway.

@entity1 @entity522 @entity5438 @entity2423 @entity3544 @entity4910 @entity584 @entity98 @entity421 @entity983 @entity10241 @entity6577 @entity496
MicroRNAs (miRNAs) are endogenous, non-coding, small RNAs, which play a critical role in regulating varieties of the biological and pathologic processes. MiR-196a has been reported to take part in tumorigenic progression of @entity421 ( @entity421 ). However, the effects of miR-196a on @entity421 are still unclear. The objective of this study is to investigate the molecular mechanism of miR-196a in @entity421 cells. In the present study, the expression of miR-196a in @entity421 cell lines was detected by real-time PCR. We found that the expression level of miR-196a was markedly up-regulated in @entity421 cell lines compared with normal osteoblastic cells. Then, the miR-196a mimic was transiently transfected into MG63 and @entity1 cells using @entity496 reagent. Subsequently, the @entity98 and Brdu-ELISA results showed that up-regulation of miR-196a promoted the cell viability and proliferation. Our results also showed that miR-196a mimic accelerated cell cycle progression of MG63 and @entity1 cells by down regulation of @entity3544 and @entity5438 , and upregulation of @entity4910 . In addition, overexpression of miR-196a suppressed apoptosis of MG63 and @entity1 cells due to increasing @entity6577 and @entity2423 expressions, and then inactivating @entity522 . Eventually, the effect of miR-196a mimic on the @entity10241 ( @entity10241 )/ @entity584 signaling pathway was explored by Western blot. From our results, transfection of miR-196a decreased the expression of @entity983 and increased the phosphorylation of @entity10241 and @entity584 . Taken together, miR-196a should be an oncogene in @entity421 . The possible mechanism was that overexpression of miR-196a promoted proliferation of MG63 and @entity1 cells by modulating the @entity983 / @entity10241 / @entity584 signaling pathway.
