The role of @entity876 and Ki67 in XXXX and the breast.

@entity0 @entity1885 @entity5 @entity4098 @entity406 @entity4261 @entity189 @entity513 @entity876 @entity15989 @entity420
OBJECTIVE: @entity4098 ( @entity4098 ) are uncommon diseases, accounting for approximately 1% of all @entity5 and 0.5-4% of all @entity0 , respectively. In 10-30% of vulval cases an invasive @entity420 is present. In such cases the disease is often aggressive and recurrence rate is high. This is in contrast to @entity4098 where the general consensus is that almost all cases are associated with an in situ or @entity513 . Our aim was to examine the presence of the @entity5 suppressor protein @entity876 and the proliferation marker Ki67 in @entity4261 and @entity4098 and correlate any differences in the expression of these two proteins with the presence of an underlying @entity189 . METHODS: Immunohistochemistry was performed on 52 archival cases of @entity4261 , which included 10 with associated invasive @entity420 , and on 37 archival cases of @entity4098 , including 26 with available associated @entity513 in situ (DCIS) or invasive @entity0 . All cases were @entity406 -fixed and paraffin wax-embedded. Monoclonal antibodies were used with microwave antigen retrieval. Streptavidin- @entity1885 -horseradish peroxidase and @entity15989 detection methods were employed to visualize antibody binding and staining. A section was scored positive for @entity876 if more than 10% of cell nuclei were stained brown and Ki67 was expressed as a percentage of positive cells to the nearest 5% of cells showing nuclear positivity (Ki67 staining index). RESULTS: @entity876 was expressed in 15 of 52 (29%) @entity4261 cases and 5 of 37 (13%) cases of @entity4098 . Four of the ten cases (40%) of @entity4261 associated with invasive disease expressed @entity876 compared with 11 of 42 (26%) cases without invasive disease. The mean Ki67 staining index for @entity4261 associated with invasion was 19%, and for that without invasion, 16%. In the breast cases, the mean staining index was 11%. CONCLUSION: Our data suggest that @entity876 may have a role to play in @entity4261 progression, and may be a late event in some cases, especially those associated with invasive disease. Ki67 has no apparent prognostic role in @entity4261 as there was no significant difference between those cases associated with and those without invasive disease. Neither @entity876 nor Ki67 appears to have a prognostic role to play in @entity4098 .
