XXXX : comparison of fluorescence in situ hybridization and reverse transcription polymerase chain reaction as adjunct diagnostic modalities.

@entity1 @entity12569 @entity5 @entity28218 @entity10366 @entity19957 @entity28219 @entity12726
UNASSIGNED: @entity28219 ( @entity28219 ) is a rare soft tissue @entity5 of intermediate biologic potential and uncertain differentiation, most often arising in the extremities of @entity1 and young adults. Although it has characteristic histologic features of a lymphoid cuff surrounding nodules of ovoid cells with blood-filled cystic cavities, diagnosis is often difficult due to its morphologic heterogeneity and lack of specific immunoprofile. @entity28219 is associated with recurrent chromosomal translocations, leading to characteristic @entity12726 - @entity10366 , @entity12726 - @entity12569 , and, rarely, @entity28218 gene fusions; fluorescence in situ hybridization (FISH), detecting @entity12726 or @entity19957 rearrangements, and reverse transcription-polymerase chain reaction (RT-PCR) for @entity12726 - @entity10366 and @entity12726 - @entity12569 fusion transcripts have become routine ancillary tools. We present a large comparative series of FISH and RT-PCR for @entity28219 . Seventeen @entity5 (from 16 @entity1 ) histologically diagnosed as @entity28219 were assessed for @entity12726 rearrangements or @entity12726 - @entity10366 and @entity12726 - @entity12569 fusion transcripts. All 17 were positive for either FISH or RT-PCR or both. Of 16, 14 (87.5%) had detectable @entity12726 - @entity10366 or @entity12726 - @entity12569 fusion transcripts by RT-PCR, whereas 13 (76.5%) of 17 had positive @entity12726 rearrangement with FISH. All 13 of 13 non- @entity28219 control @entity5 failed to show @entity12726 - @entity10366 or @entity12726 - @entity12569 fusion transcripts, whereas @entity12726 rearrangement was present in 2 of these 13 cases (which were @entity5 ). This study shows that @entity12726 - @entity10366 or @entity12726 - @entity12569 fusions predominate in @entity28219 (supporting previous reports that @entity19957 rearrangement is rare in @entity28219 ) and that RT-PCR has a comparable detection rate to FISH for @entity28219 . Importantly, cases of @entity28219 can be missed if RT-PCR is not performed in conjunction with FISH, and RT-PCR has the added advantage of specificity, which is crucial, as @entity12726 rearrangements are present in a variety of @entity5 in the histologic differential diagnosis of @entity28219 , that differ in behavior and treatment.
