Evaluation of the expressions pattern of XXXX , 21, 200c, 373 and 520c to find the correlation between epithelial-to-mesenchymal transition and @entity320 stem cell potential in @entity5 stem cells.

@entity5 @entity320 @entity17308 @entity3
UNASSIGNED: Small non-coding RNAs named microRNAs (miRNAs) modulate some functions and signaling pathways in skin epithelial cells and melanocytes. They also function as oncogenes or @entity5 suppressors in @entity5 and @entity5 @entity3 . We investigated the expression patterns of miRNAs, including @entity17308 , 21, 200c, 373 and 520c, which regulate epithelial-to-mesenchymal transition (EMT) and @entity3 in @entity5 stem cells (CSCs) and non- CSCs. Six @entity320 cell lines were tested for the expressions of stem cell markers. @entity320 stem cells were enriched via fluorescence-activated cell sorting (FACS) using the CD133 cell surface marker or spheroid culture. They were then characterized based on colony and sphere formation, and the expressions of stemness and EMT regulator genes and their invasion potential were assessed using real-time qRT-PCR and invasion assay. Our results indicate that cells enriched via sphere formation expressed all the stemness-related genes and had an enhanced number of colonies, spheres and invaded cells compared to cells enriched using the CD133 cell surface marker. Moreover, miRNAs controlling @entity3 increased in the melanospheres. This may be related to the involvement of CSCs in the metastatic process. However, this must be further confirmed through the application of knockdown experiments. The results show that sphere formation is a useful method for enriching @entity320 stem cells. Melanospheres were found to upregulate @entity17308 , 21, 200c, 373 and 520c, so we suggest that they may control both @entity3 and stemness potential.
