Expression of transforming growth factor beta ligand and receptor messenger RNAs in XXXX cell lines.

@entity11579 @entity73323 @entity641 @entity19 @entity1149 @entity2 @entity15136 @entity151 @entity11580
Specific cDNA probes for transforming growth factor beta s ( @entity73323 and @entity641 types I, II, and III receptors were used to study expression of the mRNAs of the different @entity641 ligand and @entity641 receptor isoforms in cultured @entity1149 ( @entity1149 ) cells and @entity151 ( @entity151 ) cells. Expression of @entity641 mRNA was detected in both cell types using Northern blot hybridization, with the level of expression of this mRNA being higher in several @entity1149 cell lines. In addition, expression of @entity11579 and @entity11580 mRNAs was also detected in @entity1149 and @entity151 cells but at levels that were lower than that of @entity641 mRNA. Besides expression of a 3.4-kilobase (kb) @entity11580 transcript, a smaller 2.8-kb @entity11580 transcript was detected in some @entity1149 and @entity151 cells. @entity641 and @entity11579 proteins were detected in the conditioned media of @entity1149 and @entity151 cells, with the levels being higher in several @entity1149 cells than in @entity151 cells. Expression of @entity15136 mRNAs was also detected in most @entity1149 and @entity151 cells, with expression of a 5.5-kb type I receptor mRNA being higher than that of @entity19 -kb type II receptor mRNA in both cell types. In contrast, a 6-kb @entity641 type III receptor mRNA was detected in only some @entity1149 cells and could not be detected in the @entity151 cells examined. Also, there was an inverse relationship between the level of expression of the 5.5-kb @entity641 type I receptor mRNA and that of the 6-kb @entity641 type III receptor mRNA. Addition of @entity641 and @entity11579 proteins resulted in an increase in the mRNAs for @entity641 s 1 and 2 and an increase in the amount of @entity641 protein in some @entity1149 cells, indicating that these cells are responsive to @entity641 and its effects. At the same time, a differential change in expression of the 2.8- and 3.4-kb @entity11580 transcripts was detected in some @entity2 cells following the addition of @entity641 and @entity11579 . Also, addition of @entity641 to @entity1149 cells inhibited colony formation of some of these cells in soft agarose in a dose-dependent manner.(ABSTRACT TRUNCATED AT 400 WORDS)
