Suppression of coenzyme Q       levels and the induction of multiple PDSS and COQ genes in @entity1 cells following XXXX treatment.

@entity1 @entity41384 @entity26875 @entity26 @entity70791 @entity898 @entity43054 @entity70792 @entity273 @entity26874 @entity6217 @entity7447 @entity4626 @entity855 @entity2383
Endogenous @entity7447 ( @entity7447 ) is a lipid-soluble antioxidant and essential for the electron transport chain. We previously demonstrated that @entity273 enhanced @entity7447 levels, whereas disruption of mitochondrial membrane potential by a chemical uncoupler suppressed @entity7447 levels, in @entity1 143B cells. In this study, we investigated how @entity7447 levels and expression of two PDSS and eight COQ genes were affected by @entity4626 , which inhibited @entity855 synthesis at Complex V without uncoupling the mitochondria. We confirmed that @entity4626 increased the production of reactive @entity26 species (ROS) and decreased mitochondria-dependent @entity855 production in 143B cells. We also demonstrated that @entity7447 levels were decreased by @entity4626 after 42 or 48 h of treatment, but not at earlier time points. Expression of @entity26874 and @entity26875 - @entity41384 were up-regulated after 18-hour @entity4626 treatment, and the expression of @entity6217 (PGC1-1a) elevated concurrently. Knockdown of @entity6217 down-regulated the basal mRNA levels of @entity26874 and five COQ genes and suppressed the induction of @entity70791 and @entity41384 genes by @entity4626 , but did not affect @entity7447 levels under these conditions. @entity898 suppressed the augmentation of ROS levels and the enhanced expression of @entity26875 , @entity70792 , @entity43054 , and @entity41384 induced by @entity4626 , but did not modulate the changes in @entity7447 levels. These results suggested that the condition of @entity2383 induced by @entity4626 decreased @entity7447 levels independent of oxidative stress. Up-regulation of @entity26874 and several COQ genes by @entity4626 might be regulated by multiple mechanisms, including the signaling pathways mediated by @entity6217 and ROS, but it would not restore @entity7447 levels.
