Characterization of a new monoclonal antibody to a cell surface antigen on XXXX and fetal gut tissues.

@entity1 @entity3167 @entity19 @entity5 @entity33650 @entity406 @entity787 @entity14 @entity694
@entity19 hybridoma were raised against the @entity1 @entity694 cell line CL-187. One clone was found to secrete a monoclonal antibody (ND-1) that recognizes a large external antigen ( @entity33650 ) on @entity1 @entity694 cells. With indirect immunofluorescence on @entity406 -fixed cells, more than 90% of the @entity1 @entity14 cell lines tested expressed @entity33650 . Almost all of the 46 @entity1 noncolorectal and nonhuman cell lines tested did not express @entity33650 , including @entity5 cell lines from other endodermally derived tissues. Staining of frozen sections from @entity1 @entity14 , @entity5 , normal adult, and normal fetal tissues showed expression of the antigen on @entity14 tissue, fetal colon, and fetal biliary epithelium. @entity33650 can also be detected in the serum and ascites of @entity14 @entity1 . Double indirect immunofluorescence with rabbit @entity787 ( @entity787 ) antibody and ND-1 monoclonal antibody on a @entity1 @entity14 cell line showed that @entity33650 is distinct from @entity787 . Physicochemical analysis of @entity33650 showed that it has a large molecular weight, is resistant to extraction from the cell surface, and that @entity3167 is an important component of the antigenic site. Because of the specificity for @entity14 tissue along with certain biochemical properties, @entity33650 appears to be unique when compared with other @entity5 -associated antigens. Further research is needed to define the clinical usefulness of @entity33650 in either the diagnosis or treatment of @entity14 .
