Reactivity in ELISA with DNA-loaded nucleosomes in @entity1 with proliferative XXXX .

@entity1 @entity1988 @entity1252 @entity43635
Autoantibodies against nucleosomes are considered a hallmark of @entity1252 ( @entity1252 ). We compared in @entity1 with proliferative @entity1988 the diagnostic usefulness of a dsDNA-loaded nucleosome ELISA (anti-dsDNA- @entity43635 ) with ELISAs in which dsDNA or nucleosomes alone were coated. First, we analysed whether DNA loading on nucleosomes led to masking of epitopes by using defined monoclonal anti-DNA, anti-histone and nucleosome-specific autoantibodies to evaluate the accessibility of nucleosomal epitopes in the anti-dsDNA- @entity43635 ELISA. Second, autoantibody levels were measured in these 3 ELISAs in 100 @entity1 with proliferative @entity1988 ( @entity1988 ) before immunosuppressive treatment and in 128 non- @entity1252 disease controls. In @entity1 with @entity1988 inter-assay comparisons and associations with clinical and serological parameters were analysed. The panel of monoclonal antibodies revealed that all epitopes were equally accessible in the anti-dsDNA- @entity43635 ELISA as in the two other ELISAs. @entity1 with proliferative @entity1988 were positive with dsDNA-loaded nucleosomes in 86%, with DNA in 66% and with nucleosomes in 85%. In the @entity1252 control group these frequencies were 1.6% (2 out of 128) for both the anti-dsDNA- @entity43635 and the anti-dsDNA ELISA and 0% in the anti-nucleosome ELISA. The levels in the anti-dsDNA- @entity43635 ELISA were high in a group of @entity1 with @entity1988 that showed absent reactivity in the anti-DNA or low levels in the anti-nucleosome ELISA. Anti-dsDNA- @entity43635 positivity was associated with higher SLEDAI scores within this group. Within nucleosome-based ELISAs, we propose the anti-dsDNA- @entity43635 ELISA as the preferred test system.
