XXXX potential of absorbed-retained @entity11540 residues in culture dishes on embryo development in vitro.

@entity852 @entity11540 @entity137 @entity6157 @entity10156 @entity19 @entity515 @entity1391
Four hundred eight-cell @entity19 embryos were cultured in vitro in either @entity852 (plastic) dishes (Exp. 1) or watch glasses (Exp. 2) to analyze the @entity137 potential of absorbed-retained @entity11540 ( @entity6157 ). Culture dishes were gas-sterilized with @entity6157 equipment and allowed to aerate (21 C) for varying durations. Post-sterilization @entity6157 residues, as determined by weight measurement, were eluted from @entity852 dishes at an exponential rate. After 1 wk of aeration, .625 mg @entity6157 was retained/g of @entity852 product. To determine the effect of @entity6157 residue on in vitro culture, embryos were collected from superovulated donor @entity19 (C57BL/6N), pooled in a modified Dulbecco's @entity1391 buffered saline (PBI) medium and then randomly allotted to dishes subjected to various aeration durations. Embryos were cultured in Whitten's medium +3 mg/ml bovine serum albumin (BSA) under a humidified 5% @entity515 in air atmosphere at 37 C. Gross assessments of embryo development were made using standard morphology and quality grading systems and a @entity10156 staining assay. In Exp. 1, embryo development was retarded at the 8- to 16-cell stage when less than or equal to 12 h aeration of @entity852 dishes was permitted. Aeration for 24 and 36 h resulted in suboptimal embryo development with fewer (P less than .01) blastocysts and greater degeneration rates at 48 h of in vitro culture compared with the control (manufacturer-packaged dishes, no @entity6157 ) and 1-wk aeration treatment groups.(ABSTRACT TRUNCATED AT 250 WORDS)
