An in vivo molecular response analysis of XXXX treated with Astragalus membranaceus extract.

@entity5 @entity1 @entity19 @entity14
UNASSIGNED: The fact that many chemotherapeutic drugs cause chemoresistance and side effects during the course of @entity14 treatment necessitates development of novel cytotoxic agents aiming to attenuate new molecular targets. Here, we show that Astragalus membranaceus (Fischer) Bge. var. mongolicus (Bge.) Hsiao (AM), a traditional Chinese medicine, can inhibit @entity5 growth in vivo and elucidate the underlying molecular mechanisms. The antitumor effect of AM was assessed on the subcutaneous @entity5 of @entity1 @entity14 cell line HCT116 grafted into @entity19 . The @entity19 were treated with either water or 500 mg/kg AM once per day, before being sacrificed for extraction of @entity5 , which were then subjected to microarray expression profiling. The gene expression of the extraction was then profiled using microarray analysis. The identified genes differentially expressed between treated @entity19 and controls reveal that administration of AM suppresses chromosome organization, histone modification, and regulation of macromolecule metabolic process. A separate analysis focused on differentially expressed microRNAs revealing involvement of macromolecule metabolism, and intracellular transport, as well as several @entity5 signaling pathways. For validation, the input of the identified genes to The Library of Integrated Network-based Cellular Signatures led to many chemopreventive agents of natural origin that produce similar gene expression profiles to that of AM. The demonstrated effectiveness of AM suggests a potential therapeutic drug for @entity14 .
