Evaluation of a @entity1 ovarian follicle isolation technique to obtain disease-free follicle suspensions before safely grafting to XXXX @entity1 .

@entity5 @entity1 @entity1778 @entity1861
OBJECTIVE: To evaluate the safety of our follicle isolation procedure in a model of ovarian tissue artificially contaminated with @entity5 cells, then to improve the procedure to effectively eliminate malignant cells from follicle suspensions without altering viability. DESIGN: Prospective experimental study. SETTING: Gynecology research unit in a university hospital. @entity1 (S): Ten @entity1 undergoing laparoscopy for benign gynecologic disease. INTERVENTION(S): Follicle isolation from ovarian tissue artificially contaminated with marked fluorescent @entity1778 cells, either by the usual pickup technique without further treatment (group 1) or by washing three times after pickup (group 2). MAIN OUTCOME MEASURE(S): Evidence of @entity1778 cells in follicle suspensions using fluorescence microscopy and quantitative real-time polymerase chain reaction, and analysis of follicle viability. RESULT(S): In group 1, 196 @entity1778 cells were detected by fluorescence microscopy out of 499 follicles retrieved, while just one @entity1778 cell was found among 772 follicles after three washes. The @entity1861 fusion transcript was detected when at least 19 cells were present in follicle suspensions; four samples were positive in group 1, and all were negative in group 2. Follicle viability was similar in both groups (95.6% vs. 96.4%). CONCLUSION(S): @entity5 cells could inadvertently be picked up with isolated follicles in case of malignant contamination of ovarian tissue. A simple purging procedure consisting of three washes proved effective for eliminating @entity1778 cells while maintaining good follicle viability.
