Depletion of CD8+ T Cells Exacerbates XXXX + T Cell-Induced Monocyte-to-Fibroblast Transition in @entity8 .

@entity8 @entity82 @entity19986 @entity19 @entity1961 @entity58
UNASSIGNED: Bone marrow-derived monocyte-to-fibroblast transition is a key step in @entity8 pathogenesis, which is regulated by the inflammatory microenvironment. However, the mechanism by which the inflammatory microenvironment regulates this transition is not fully understood. In this study, we examined how the CD8(+) T cell/IFN-y microenvironment regulates the monocyte-to-fibroblast transition in @entity8 . Genetic ablation of CD8 promoted a monocyte-to-fibroblast transition and increased @entity8 , whereas reconstitution of CD8 knockout (KO) @entity19 with CD8(+) T cells decreased @entity82 . However, depletion of @entity1961 (+) T cells in CD8 KO @entity19 also reduced @entity82 . To elucidate the role of @entity1961 (+) T cells in mediating CD8-regulated monocyte-to-fibroblast transition, @entity1961 (+) T cells were isolated from obstructed kidneys of CD8 KO or wild-type @entity19 . @entity1961 (+) T cells isolated from CD8 KO obstructed kidney expressed more @entity58 and @entity19986 and less IFN-y and T-bet and showed increased monocyte-to-fibroblast transition in vitro compared with those isolated from wild-type obstructed kidney. To examine the role of IFN-y-expressing CD8(+) T cells, we reconstituted CD8 KO @entity19 with CD8(+) T cells isolated from IFN-y KO @entity19 . The IFN-y KO CD8(+) cells had no effect on @entity58 , @entity19986 , IFN-y, and T-bet mRNA expression in obstructed kidneys or @entity8 . Taken together, our findings identify the axis of CD8(+) T cells and IFN-y- @entity1961 (+) T cells as an important microenvironment for the monocyte-to-fibroblast transition, which negatively regulates @entity8 .
