XXXX telomeric proteins occupy selective interstitial sites.

@entity1 @entity7072 @entity35762 @entity7070 @entity7067 @entity7071 @entity7073
@entity1 telomeres are bound and protected by protein complexes assembled around the six core telomeric proteins @entity7073 , @entity7071 , @entity7072 , @entity7067 , @entity35762 , and @entity7070 . The function of these proteins on telomeres has been studied extensively. Recently, increasing evidence has suggested possible roles for these proteins outside of telomeres. However, the non-canonical (extra-telomeric) function of @entity1 telomeric proteins remains poorly understood. To this end, we systematically investigated the binding sites of telomeric proteins along @entity1 chromosomes, by performing whole-genome chromatin immunoprecipitation (ChIP) for @entity7073 and @entity7072 . ChIP sequencing (ChIP-seq) revealed that @entity7073 and @entity7072 could be found on a small number of interstitial sites, including regions that are proximal to genes. Some of these binding sites contain short telomere repeats, suggesting that telomeric proteins could directly bind to interstitial sites. Interestingly, only a small fraction of the available interstitial telomere repeat-containing regions were occupied by @entity7073 and @entity7072 . Ectopically expressed @entity7072 was able to occupy additional interstitial telomere repeat sites, suggesting that protein concentration may dictate the selective targeting of telomeric proteins to interstitial sites. Reducing @entity7073 and @entity7072 expression by RNA interference led to altered transcription of @entity7073 - and @entity7072 -targeted genes. Our results indicate that @entity1 telomeric proteins could occupy a limited number of interstitial sites and regulate gene transcription.
