Rapid determination of the antimycotic drug XXXX in human serum by micellar electrokinetic capillary chromatography with direct sample injection.

@entity1 @entity19131 @entity3208 @entity2379 @entity10498 @entity4895 @entity1391 @entity3440
The rapid determination of @entity4895 ( @entity10498 ) in human serum by micellar electrokinetic capillary chromatography (MECC) with direct sample injection is discussed. Minute (nanoliter) quantities of @entity1 sera are applied to the beginning of a fused @entity3440 capillary filled with a @entity1391 / @entity19131 buffer (pH 9.2) containing 75 mM @entity3208 . Upon application of an electric field along the capillary, endogenous and drug substances are transported toward the cathode and separated into distinct zones that are detected by on-column ultraviolet absorption. Depending on the capillary and instrument used, 5-FC is shown to elute within 2-3.5 min of current application and free of endogenous interferences. 5-FC becomes also well separated from another often coadministered antimycotic drug, @entity2379 . Thus, quantitation of 5-FC is accomplished without any sample pretreatment. MECC data of 60 @entity1 sera produced on two different automated instruments and 5-FC serum levels obtained by an agar-based bioassay are shown to agree well. For MECC, intraday and interday reproducibility data (80 micrograms/ml level) are shown to be < or = 4.5 and < 7%, respectively. Due to short run times and short capillary equilibration time intervals between runs, a sample throughput of 15/h is feasible. Thus, this technology is suitable for rapid determination of 5-FC serum levels, the time requirement for running a complete calibration, two control sera, and several @entity1 samples being approximately 1 h only.
