Overlapping roles of endothelial selectins and XXXX in immune complex-induced leukocyte recruitment in the cremasteric microvasculature.

@entity19 @entity32 @entity24780 @entity6580
Many adhesion molecule pathways have been invoked as mediating leukocyte recruitment during immune complex-induced @entity32 . However the individual roles of these molecules have not been identified via direct visualization of an affected microvasculature. Therefore, to identify the specific adhesion molecules responsible for leukocyte rolling and adhesion in immune complex-dependent @entity32 we used intravital microscopy to examine postcapillary venules in the @entity19 cremaster muscle. Wild-type @entity19 underwent an intrascrotal reverse-passive Arthus model of immune complex-dependent @entity32 and subsequently, leukocyte-endothelial cell interactions and P- and @entity24780 expression were assessed in cremasteric postcapillary venules. At 4 hours, the reverse-passive Arthus response induced a significant reduction in leukocyte rolling velocity and significant increases in adhesion and emigration. P-selectin expression was increased above constitutive levels whereas @entity24780 showed a transient induction of expression peaking between 2.5 to 4 hours and declining thereafter. While @entity24780 was expressed, rolling could only be eliminated by combined blockade of P- and @entity24780 . However, by 8 hours, all rolling was P-selectin-dependent. In contrast, inhibition of @entity6580 had a minimal effect on leukocyte rolling, but significantly reduced both adhesion and emigration. These observations demonstrate that immune complex-mediated leukocyte recruitment in the cremaster muscle involves overlapping roles for the endothelial selectins and @entity6580 .
