Enhancing the detection of XXXX cysts in foods using inertial microfluidic separation.

@entity461 @entity45 @entity12681
UNASSIGNED: The sensitivity and specificity of current @entity45 detection methods for foods are largely determined by the effectiveness of the elution, separation and concentration methods used. The aim of these methods is to produce a final suspension with an adequate concentration of @entity45 for detection, and a low concentration of interfering food debris. In the present study, a microfluidic device, which makes use of inertial separation, was designed and fabricated for the separation of @entity45 . A cyclical pumping platform and protocol was developed to concentrate 10 ml suspensions down to less than 1 ml. Tests involving @entity461 cysts and 1.90  m microbeads in pure suspensions demonstrated the specificity of the microfluidic chip for cysts over smaller non-specific particles. As the suspension cycled through the chip, a large number of beads were removed (70%) and the majority of the cysts were concentrated (82%). Subsequently, the microfluidic inertial separation chip was integrated into a method for the detection of @entity461 cysts from lettuce samples. The method greatly reduced the concentration of background debris in the final suspensions (10-fold reduction) in comparison to a conventional method. The method also recovered an average of 68.4% of cysts from 25 @entity12681 samples, and had a limit of detection (LOD) of 38 cysts. While the recovery of cysts by inertial separation was slightly lower, and the LOD slightly higher, than the conventional method, the sample analysis time was greatly reduced as there were far fewer background food particles interfering with the detection of cysts by immunofluorescence microscopy.
