Effects of @entity5 necrosis factor alpha and melphalan on the cytokine production of circulating T cells in XXXX with @entity5 .

@entity1 @entity308 @entity5 @entity310 @entity4515 @entity1359 @entity1198 @entity1360 @entity379 @entity1199 @entity3646 @entity1361
BACKGROUND: The objective of the present study was to investigate the effects of isolated limb perfusion (ILP) with @entity5 necrosis factor alpha ( @entity310 ) and melphalan on circulating T cells from @entity5 @entity1 using two different methods. DESIGN: Eight @entity1 undergoing an ILP entered the study. At first, the number of T cells at several time points was determined using FACScan. Subsequently, production of @entity379 ) ( @entity3646 (T-helper 2) was measured at the intracytoplasmic level after stimulation with @entity1359 ( @entity4515 ) and @entity1360 . @entity379 , @entity3646 and @entity1199 (also T-helper 1) production in the whole-blood cell culture system was then determined after stimulation with a combination of anti-CD3/anti- @entity1361 monoclonal antibodies. RESULTS: An enormous decrease in the number of circulating T cells was observed. In the remaining T-cell population cytokine production ( @entity1199 , @entity3646 , @entity379 ) was @entity308 , showing the same pattern in both methods. No difference could be detected between the effect of @entity310 and melphalan on @entity1198 cells and Th2 cells. CONCLUSIONS: The results demonstrate that @entity310 and melphalan reduce the number of circulating T cells and at the single-cell level decrease cytokine production in the remaining circulating T cells. No selective effect of @entity310 on @entity1198 or Th2 cells could be detected. If the impaired T-cell function is representative of all T cells remaining in the systemic circulation, this could help to explain the tolerability of high TNF concentrations after ILP, perhaps by decreasing the synthesis and production of T-cell-derived cytokines.
