Adoptive transfer of experimental autoimmune @entity4643 in XXXX . Immunopathogenic mechanisms and histologic features.

@entity3145 @entity18295 @entity1600 @entity4643 @entity35
In order to learn about the immunopathogenic mechanisms of experimental autoimmune @entity4643 (EAU), the capacity of lymphocytes to transfer the disease was studied. EAU was transferred to naive syngeneic @entity35 by intraperitoneal injection of spleen or @entity1600 ( @entity1600 ) cells from @entity18295 immunized @entity35 , following their incubation in culture with either @entity18295 or concanavalin A (Con A). In contrast, the same cells did not cause inflammatory changes in the recipient eyes when injected intravitreally. The identity of the lymphocytes that transfer EAU was determined by using monoclonal antibody enriched subsets of lymphocytes. EAU was transferred by the subset of helper/inducer T-cells, but not by T-cells of the suppressor/cytotoxic subset. Recipient @entity35 of spleen or @entity1600 cells cultured with @entity18295 exhibited both humoral and cellular immune responses to @entity18295 . On the other hand, recipients of spleen cells cultured with Con A developed only cellular immune response to @entity18295 . Yet, both groups of recipients fully developed EAU. The clinical and histologic changes in recipient @entity35 closely resembled those in @entity35 in which EAU was induced by active immunization. Severe @entity3145 occurred at the photoreceptor cell layer, but inflammatory infiltration also was found in other ocular tissues. Involvement of polymorphonuclear leukocytes (PMNs) was noted throughout ocular tissues even in the eyes of recipients with no detectable antibodies to @entity18295 , suggesting that the ocular PMNs infiltration in @entity35 is not necessarily the result of an Arthus-like inflammatory process.
