Characterization of the antigenicity of the XXXX in @entity775 .

@entity1 @entity775 @entity35 @entity70835
@entity1 @entity70835 ( @entity70835 ) is the autoantigen recognized by anti-liver cytosol type 1 (LC1) autoantibodies in @entity775 ( @entity775 ) @entity1 . In @entity35 , this octameric protein is localized on the Golgi apparatus and binds brain microtubules (MTs) and vimentin. Subcellular localization of @entity1 @entity70835 and its implication in the pathogenesis of @entity775 are unknown. Localization of the @entity1 @entity70835 in @entity1 hepatocytes was done using indirect immunofluorescence and subcellular fractionations followed by in vitro binding techniques. The @entity70835 antigen at two distinct locations in hepatocytes, free in the cytosol and associated with the Golgi membranes are recognized by anti-liver cytosol type 1 autoantibodies. The @entity1 @entity70835 binds reversibly to the Golgi membranes and this complex formation is increased by anti-liver cytosol type 1 autoantibodies. Finally, @entity1 @entity70835 does not interact with liver-specific cytoskeleton proteins. Anti-liver cytosol type 1 autoantibodies are directed against the mature high molecular form of @entity1 @entity70835 . Therefore, the subcellular location of the protein may influence the production of autoantibodies and their role in the pathogenesis of @entity775 . This antigen-driven response does not appear to be facilitated or enhanced by a possible interaction between @entity1 @entity70835 and hepatocyte cytoskeleton proteins.
