Isolation of low density lipoprotein from atherosclerotic vascular tissue of XXXX @entity95 .

@entity19 @entity4291 @entity95 @entity8577 @entity2434 @entity59386 @entity2975 @entity1043 @entity214 @entity8557 @entity165 @entity597
Atherogenic properties of low density lipoproteins (LDL) in vivo may reflect modification of lipoproteins associated with endothelial translocation and exposure to extracellular matrix and interstitial fluid. To examine whether modifications of LDL occur in vivo, lipoproteins were isolated from plasma and vascular tissue of @entity8577 ( @entity8577 ) @entity95 . LDL was extracted from vascular tissue (LDL-V) by homogenization in the presence of a @entity1043 buffer. Control experiments demonstrated that modifications did not occur under the preparative conditions used to release LDL from tissue. LDL-V contained less esterified @entity165 , but more @entity8557 , than did LDL from plasma (LDL-P). The diameters of both LDL-V and LDL-P followed gaussian distributions, but LDL-V particles were smaller (20.3 +/- 0.1 and 26.3 +/- 0.1 nm). Mild lipid peroxidation was evident in LDL-V. The @entity4291 content was increased in LDL-V, with less @entity2434 than in LDL-P. Sodium dodecyl @entity597 - @entity2975 gel electrophoresis ( @entity214 -PAGE) indicated that @entity59386 was depleted in LDL-V, but Western blot analyses identified lower molecular weight proteins antigenically related to @entity59386 . LDL-V markedly stimulated @entity165 esterification in @entity19 peritoneal macrophages and also in @entity95 alveolar macrophages, a cell type that did not respond to acetylated LDL. LDL-V was not recognized by cultured @entity95 skin fibroblasts. Thus, LDL isolated from atherosclerotic vascular tissue in vivo was modified in a fashion that could confer atherogenic properties reflected by augmentation of @entity165 esterification in macrophages in vitro.
