@entity19 XXXX RL male 1 and @entity242 Meth A antigens recognized by cytotoxic T lymphocytes (CTL).

@entity137 @entity242 @entity5 @entity70611 @entity19 @entity60 @entity3783 @entity1778 @entity1961 @entity1968
Peptide elution and expression cloning methods have been used to identify T cell-recognized antigens for which no molecular information is available. We identified a unique @entity5 antigen peptide pRL1a, IPGLPLSL that is recognized by CTL on BALB/c RL male 1 @entity1778 by peptide elution. The sequence of the peptide corresponded to the normally untranslated 5' region of akt. @entity137 was generated in BALB/c spleen cells by in vivo and in vitro sensitization with pRL1a peptide in the form of multiple antigen peptide (MAP), but not the original form. @entity70611 a MAP immunization had a significant growth-inhibitory effect. @entity70611 a MAP was mostly internalized into the endosomal compartment of antigen-presenting cells, leaked to the cytosol, and degraded, and the pRL1a peptide produced was presented through the MHC class I pathway. In vivo depletion of @entity1961 T cells from @entity5 -inoculated BALB/c @entity19 caused RL male 1 regression. Overexpression of the RLakt molecule seemed to induce @entity1961 immunoregulatory cells, which resulted in progressive RL male 1 growth in BALB/c @entity19 . In vivo administration of anti- @entity3783 mAb (PC61) caused regression of RL male 1, suggesting that @entity1961 (+) @entity3783 (+) immunoregulatory cells were involved in the @entity5 growth. Recently, we improved the sensitivity and the efficacy of T cell antigen cloning from cDNA expression libraries by using large- and small-scale ELISPOT assays. Using the @entity60 ELISPOT method, we obtained a cDNA clone S35 of 937 bp recognized by AT-1 CTL on BALB/c Meth @entity242 . S35 was a part of the @entity1968 -regulated nuclear matrix-associated protein (ramp). AT-1 CTL recognized the peptide LGAEAIFRL, which was derived from a newly created open reading frame due to the exon 14 extension.
