Apoptosis in XXXX cells treated with PDT.

@entity1 @entity522 @entity5 @entity515 @entity11 @entity3460 @entity463
BACKGROUND: Despite significant advances in neurosurgical techniques, the median survival time of @entity1 with @entity463 has improved little over the past 50 years and remains less than one year. Photodynamic therapy (PDT) is presently established as a widely accepted modality for the treatment of a variety of solid @entity5 . OBJECTIVES: This study evaluated the effect of PDT-Photogem(  ) on five @entity11 cell lines (U87, U138, U251, U343, and T98G). METHODS: The experiments were carried out in 25-cm(3) flasks with different groups of cells seeded at a density of 1   *   10(5) cells per flask. After 3   h, the medium was removed, and the cells were incubated for 4   h with Photogem (5    g/mL). After the incubation time, the photosensitizer-containing medium was removed and the cells were irradiated with LED (630   nm, 25   mW/cm(2), 25   J/cm(2)) devices for 17   min. For the final steps of the PDT, the cells were returned to the incubator and kept at 37  C with 5% @entity515 for 24   h, the cell viability assay was assessed using the trypan blue method, and the expression of @entity522 mRNA levels was assessed by real-time quantitative PCR. RESULTS: Upon PDT-Photogem(  ) treatment, viable cells, as evaluated by the @entity3460 dye-exclusion method, decreased in two cell lines (U87 and U138) but not in the other three. Apoptosis, as assessed by the expression of @entity522 mRNA levels, was at least partly involved in the death mechanism of the cell lines. CONCLUSIONS: Collectively, our results indicated that PDT-Photogem(  ) can act in @entity11 cells, thus encouraging new experiments in this field.
