Evidence for a protective response by the gill proteome of XXXX exposed to X-ray induced bystander signals.

@entity5660 @entity4059 @entity5 @entity26 @entity3006 @entity2421 @entity880 @entity2388
The bystander effect occurs when cells which are not directly exposed to radiation, but which receive signals from irradiated cells, respond as though they were irradiated. An X-ray induced bystander effect has been demonstrated in @entity5660 gills. Therefore, a proteomic comparison was made of gills from X-ray treated trout and trout exposed to X-ray induced bystander signals. 2-D gel analysis revealed X-ray exposure increased the expression of the @entity5 related protein annexin II. The proteomic changes associated with the bystander effect differed from those associated with direct radiation exposure. Expression of a hemopexin-like protein, Rho @entity3006 dissociation inhibitor (RhoGDI) and @entity2388 dehydrogenase (PDH) were increased. These proteins possess protective properties against reactive @entity26 damage (a component of the bystander signal), regulate epithelial polarity and prevent @entity880 acidosis, respectively. There was also evidence for an increase in chromosome 1 @entity4059 ( @entity4059 ) protein turnover, which could suggest the protective response is transcriptionally regulated. The freshwater fish gill is a polarised barrier, separating against an external @entity2421 environment. Since the maintenance of epithelial polarity is vital to gill function, these bystander effect proteomic changes could collectively protect the structural, functional and intracellular integrity of gill epithelia.
