Patterns of inflammatory vascular endothelial changes in XXXX liver grafts.

@entity12830 @entity19 @entity22140 @entity12829 @entity6580
We have investigated the vascular endothelial phenotypes found at various times posttransplant in @entity19 B10-->C3H liver grafts. In this model, liver allografts are spontaneously accepted, and survive indefinitely unless the recipient is first allosensitized with a skin allograft, in which case the liver allografts are rejected within five days. In our previous studies, allograft inflammation was associated with the development of vascular endothelial reactivity with the mAbs @entity12830 and M/ @entity12829 (anti- @entity6580 ). We observed that vascular endothelia in both liver isografts and allografts develop reactivity with @entity12830 mAb within two days of transplantation, indicating endothelial activation in both situations. In contrast, only the endothelia in liver allografts develop @entity6580 expression, as detected with M/ @entity12829 mAb. @entity6580 was expressed in both rejecting and accepting liver allografts, demonstrating that endothelial @entity6580 expression is indicative of ongoing graft inflammation but not necessarily graft rejection. Liver parenchymal cells did not appear to develop reactivity with either antibody under any of the conditions tested. In contrast, bile duct epithelia developed M/ @entity12829 reactivity ( @entity6580 expression), but not @entity12830 reactivity in liver allografts, but not isografts. These data demonstrate @entity22140 -dependent and alloantigen-independent patterns of endothelial behavior in @entity19 liver grafts that are quite similar to those found in @entity19 cardiac grafts. Further, they demonstrate that the expression of @entity6580 by graft endothelia is not diagnostic for acute rejection of liver allografts.
