Immune complexome analysis of serum and its application in screening for immune complex antigens in XXXX .

@entity1 @entity3499 @entity7110 @entity571 @entity18042 @entity5 @entity281 @entity792 @entity309
BACKGROUND: Analysis of @entity7110 ( @entity7110 ) produced during an immune response may be useful in elucidating some aspects of this process. Identification of antigens incorporated into @entity7110 provides information that may be helpful in developing diagnostic and treatment strategies for @entity792 , @entity281 , @entity5 , and transplantation therapy, and such information might be more relevant than information on free antigens. Because @entity7110 may contain many antigens, comprehensive identification and profiling of such antigens is more effective than immunoblotting detection. METHODS: We developed a novel proteomic strategy (immune complexome analysis) in which immune complexes (ICs) are separated from serum, digested directly with trypsin, and then subjected to nano-liquid chromatography-tandem mass spectrometry for identifying and profiling antigens in @entity7110 . We applied this strategy to the analysis of @entity7110 in 21 @entity309 ( @entity309 ) patients. Serum samples from 13 healthy donors and 8 @entity571 @entity1 were used as controls. RESULTS: @entity7110 containing @entity3499 ( @entity3499 ) and @entity18042 ( @entity18042 ) were found in the serum of 81% and 52% of @entity309 @entity1 , respectively, and in none of the controls. CONCLUSIONS: The ICs in the serum of a majority of the @entity309 @entity1 contained @entity3499 or @entity18042 , and these ICs may have potential as alternative biomarkers. Our technique for immune complexome analysis uses routine clinical samples, simple protocols, and widely available equipment. This method may be generally applicable to the study of the relationship between @entity7110 and certain diseases associated with the immune response in animals and @entity1 .
