Functional analysis of the cag pathogenicity island in @entity1534 isolates from @entity1 with XXXX , peptic ulcer, and @entity356 .

@entity1 @entity7242 @entity1819 @entity3621 @entity1534 @entity2757 @entity687 @entity669 @entity15206 @entity356
@entity1534 is the causative agent of a variety of @entity1819 , but the clinical relevance of bacterial virulence factors is still controversial. Virulent strains carrying the cag pathogenicity island (cagPAI) are thought to be key players in disease development. Here, we have compared cagPAI-dependent in vitro responses in @entity1534 isolates obtained from 75 @entity1 with @entity3621 , peptic ulcer, and @entity356 (n = 25 in each group). @entity15206 gastric epithelial cells were infected with each strain and assayed for (i) @entity2757 expression, (ii) translocation and @entity669 phosphorylation of @entity2757 , (iii) @entity7242 inactivation, (iv) cortactin dephosphorylation, (v) induction of actin cytoskeletal rearrangements associated with cell elongation, (vi) induction of cellular motility, and (vii) secretion of @entity687 . Interestingly, we found high but similar prevalences of all of these cagPAI-dependent host cell responses (ranging from 56 to 80%) among the various groups of @entity1 . This study revealed @entity2757 proteins with unique features, @entity2757 subspecies of various sizes, and new functional properties for the phenotypic outcomes. We further showed that induction of @entity15206 cell motility and elongation are two independent processes. Our data corroborate epidemiological studies, which indicate a significant association of cagPAI presence and functionality with histopathological findings in @entity3621 , peptic ulcer, and @entity356 @entity1 , thus emphasizing the importance of the cagPAI for the pathogenicity of @entity1534 . Nevertheless, we found no significant association of the specific @entity1534 -induced responses with any particular @entity1 group. This may indicate that the determination of disease development is highly complex and involves multiple bacterial and/or host factors.
