Immunological analyses of antigens associated with experimental XXXX .

@entity35 @entity19 @entity27774 @entity3252 @entity3204
By immunoelectrophoresis of periotoneal exudates and @entity3204 cell antigens from @entity19 and hamsters infected with @entity3204 , using their corresponding rabbit antisera absorbed with serum of uninfected animals, eleven precipitating antigens were distinguished and named No. 1--11 in order of increasing electrophoretic mobility. Monospecific rabbit antisera against two of these antigens (antigen No. 1 and 11) were prepared by immunization with antigen purified by zone electrophoresis, and were used for immunochemical analyses: the component with higher mobility on immunoelectrophoresis, antigen No. 11, was detectable only in association with @entity3204 infection of @entity19 , hamsters, @entity35 and guinea pigs. Antigen No. 11 was also detectable as a single major antigen in the medium of cultures of L cells infected with @entity27774 . In contrast, antigen No. 1 was detectable in uninfected @entity19 in peritoneal exudates, and homogenates of thymus, liver and spleen, but not of brain or lymphocytes. In the latex agglutination test (LAT) for @entity3252 , anti-No. 1 serum gave a positive reaction, but anti-No. 11 gave a negative one. In 31 anti- @entity3252 sera examined, there was no correlation between the number of precipitating antibodies detectable by immunoelectrophoresis and the LAT titer.
