Localization of postresection XXXX receptor expression using laser capture microdissection.

@entity19 @entity6354 @entity1373 @entity12841
BACKGROUND/PURPOSE: @entity6354 ( @entity6354 ) and its receptor ( @entity12841 ) are key components in the genesis of adaptation after small bowel resection (SBR). Within intestinal homogenates, @entity12841 expression is increased after SBR; however, the exact cells responsible for altered @entity12841 expression are unknown. In this study, laser capture microdissection (LCM) microscopy was used to elucidate the specific cellular compartment(s) responsible for postresection changes in @entity12841 expression. METHODS: Male ICR @entity19 underwent a 50% proximal SBR or sham operation. After 3 days, frozen sections were taken from the remnant ileum. Individual cells from villi, crypt, muscularis, and mesenchymal compartments were isolated by LCM. @entity12841 mRNA expression for each cell compartment was quantified using real-time polymerase chain reaction (PCR). RESULTS: @entity12841 expression was increased after SBR within the crypt (2-fold) and muscularis compartments (3-fold). There were no changes detected after SBR in the villus tips or mesenchymal compartments. CONCLUSIONS: Increased expression of @entity12841 in crypts directly correlates with the zone of cell proliferation and supports the hypothesis that @entity12841 signaling is crucial for the mitogenic stimulus for adaptation. The finding of increased @entity12841 expression in the muscular compartment is novel and may implicate a role for @entity12841 as a mediator of the @entity1373 seen after massive SBR.
