Drug protein binding in XXXX : evaluation of nine drugs.

@entity1 @entity1169 @entity2544 @entity4444 @entity3175 @entity35620 @entity3159 @entity1219 @entity177 @entity3174 @entity2645 @entity1011 @entity1338 @entity1499
In this study, changes of protein binding of nine drugs were evaluated. In addition, @entity2544 and @entity3174 , the two drugs with the most substantial and progressive decrease in protein binding, were further studied by high performance liquid chromatography (HPLC)-fractions of ultrafiltrate of normal and uremic serum, in an attempt to identify substances causing drug protein binding inhibition. There was a marked decline of the protein binding of @entity2544 , @entity3174 and @entity1499 (dialyzed @entity1 vs. normals: -20.1, -16.0 and -15.1%, respectively). There was a rise in the protein binding of @entity1169 , @entity4444 and @entity1338 . The changes observed for @entity3175 , @entity3159 and @entity2645 were less marked. For @entity3174 , @entity2544 , @entity1499 and @entity3175 , protein binding was inversely correlated to the serum @entity1011 (r = 0.87, 0.80, 0.79 and 0.67, P less than 0.001), and a less pronounced but still significant positive correlation was found for @entity1338 (r = 0.46, P less than 0.01). Ultrafiltrate, obtained during a hemofiltration session, inhibited protein binding of @entity2544 and @entity3174 in a dose dependent way. After separation of this ultrafiltrate by HPLC, it appeared that for both @entity2544 and @entity3174 at least a part of this inhibitory activity corresponded to the elution zone of @entity35620 . For @entity2544 two other inhibitory zones were further recognized: one corresponding to the elution zone of @entity1219 and one in which the responsible substance remained unidentified. @entity35620 in solution inhibited protein binding of @entity2544 and @entity3174 in a dose dependent way. In conclusion, protein binding of several drugs currently used in @entity177 is affected in parallel with renal function, which might affect the therapeutic effectiveness of the drugs.(ABSTRACT TRUNCATED AT 250 WORDS)
