Prognostic Implication of Lymphovascular Invasion Detected by Double Immunostaining for D2-40 and MITF1 in Primary XXXX .

@entity1 @entity4353 @entity1711 @entity1066 @entity1600 @entity1710 @entity3 @entity320
BACKGROUND: Lymphovascular invasion (LVI) is associated with adverse outcomes in primary @entity320 (PCM). Detection of LVI by @entity1710 and @entity1711 staining alone is 0%-6%, but targeting lymphovascular structures increases the detection rate. OBJECTIVE: To examine the prognostic significance of LVI detected by immunostaining for D2-40 and @entity4353 -associated transcription factor 1 (MITF1) in PCM. METHODS: The authors retrospectively analyzed 120 PCM samples. We compared the LVI detection rates of immunostaining for D2-40 only (22%), double staining for D2-40 and MITF1 (38%), and @entity1710 and @entity1711 , and examined the association of LVI with clinicopathologic variables and clinical outcomes. RESULTS: Immunolabeling with both methods significantly increased the LVI detection rate. Double staining for D2-40 and MITF1 as well as D2-40-detected LVI was significantly associated with increased Breslow thickness, number of mitoses, and @entity1600 ( @entity1600 ) @entity3 . D2-40-detected LVI was also associated with @entity1066 . Although the difference was not significant, double staining for D2-40 and MITF1 allowed for easier detection of LVI than D2-40 alone. LIMITATIONS: This study was conducted in a tertiary referral institution; therefore, a referral bias cannot be excluded. CONCLUSIONS: Immunolabeling increased detection of LVI in PCM. Because LVI is a positive predictive marker for @entity1600 @entity3 , the authors propose using anti-D2-40 and anti-MITF1 in the evaluation of LVI in @entity1 with PCM with a certain risk of @entity1600 @entity3 .
