Gene expression profiling of XXXX receptor and ligand-dependent transcription.

@entity72214 @entity0 @entity12251 @entity470 @entity1135 @entity42834 @entity15633 @entity15774 @entity1722
Overexpression of @entity1722 and @entity12251 receptors in @entity0 may be accompanied by contrasting clinical outcomes. To investigate the molecular mechanisms contributing to these differences, we undertook a comparative study of gene expression regulated by the two receptors. Agonistic antibodies were employed to activate @entity1722 and @entity12251 in isolation from the other ErbBs in @entity0 cells. Gene expression profiling using a 16 755-gene oligonucleotide array was performed to identify transcriptional targets of receptor activation. Our results indicate that, in the same cell line, @entity1722 and @entity12251 activation influence gene transcription differentially. Although there are genes that are regulated by signaling from both receptors, there are also receptor-specific targets that are preferentially regulated by each receptor. We further show that two ligands acting via the same receptor homodimer may activate different subsets of genes. Many of the induced genes are hitherto unidentified targets of @entity470 signaling. These include @entity12251 targets @entity72214 , @entity15774 , and @entity15633 and @entity1722 -activated @entity1135 / @entity1135 and @entity42834 . Targets of @entity1722 homodimer signaling may be especially important as markers in @entity0 , where @entity1722 homodimerization mediated by overexpression and ligand-independent activation is common.
