Immunocytochemical localization of insulin-immunoreactive cells in the pancreatic ducts of XXXX treated with trypsin inhibitor.

@entity1184 @entity1373 @entity35 @entity1460
It is well known that @entity1460 trypsin inhibitor exerts trophic effects on the exocrine pancreas, resulting in the @entity1184 of acinar cells. Some evidence also exists for @entity1373 in acinar tissue, the ductal epithelium and islet tissue. @entity35 maintained for 3 weeks on an oral administration of @entity1460 trypsin inhibitor (200 mg/50 ml drinking water) were compared with untreated animals. Significant changes were noted in treated animals (p less than 0.01). Trypsin inhibitor-treated @entity35 showed an increase in pancreatic weight (2.33 +/- 0.46 g). The volume ratio of acinar, islet and connective tissue as measured by the stereology point-count technique remained the same in both groups. Ductal tissue, however, exhibited an increase in volume ratio, 3.77 +/- 4.38% per 2714 micron2 area of tissue, in trypsin inhibitor-treated animals. All tissue components showed an increase in the experimental animals: acinar (125%), islet (144%), ductal (325%) and connective tissue (94%). Increased size of acinar cell nuclei, as measured by average cord length, 6.20 +/- 0.13 micron, and a decreased nuclear density of acinar cells, 28 +/- 4.74 per 150 micron2 area of tissue, indicated @entity1184 changes in these cells of the experimental animals. Using immunohistochemical localization and the point-count technique, a significant fraction of the total pancreatic volume in experimental animals was represented by ducts containing immunoreactive cells. The percent of volume ratio, 0.42 +/- 0.15% per 2714 micron2 area of tissue, was calculated for ducts containing insulin-immunoreactive cells within their epithelium.
