Analysis of XXXX , @entity3289 and @entity794 levels in edentulous mouths prior to and 6  months after placement of one-piece zirconia and titanium implants.

@entity1 @entity28558 @entity794 @entity737 @entity3289 @entity2523
BACKGROUND: It has been suggested that completely edentulous @entity1 harbour fewer periodontopathic bacteria compared with dentate @entity1 , due to the removal of the subgingival periodontal environment. However, reappearance of certain microbes has been reported after the placement of implants in these @entity1 . AIM: The aim of this study was to determine whether the periodontopathic bacteria @entity2523 and @entity3289 , as well as the non-periodontopathic bacterium, @entity794 , emerged in edentulous @entity1 6  months after placement of one-piece zirconia and titanium implants. MATERIALS AND METHODS: Twenty-six @entity1 were included in the study (titanium  =  13, zirconia  =  13). Microbial samples were collected from the tongue prior to implant placement and 6  months after implant placement from both the tongue and from around the implants. A qRT-PCR assay using SYBR green/ROX chemistry was used for the detection and quantification of rgp, @entity28558 and karilysin single-copy gene of @entity2523 , @entity3289 and @entity794 , respectively. Positive controls used in the study were pure bacterial gDNA purified from cultures of @entity2523 and @entity794 , a cloned sequence of the karilysin gene for @entity3289 , a plaque sample positive for @entity2523 and @entity3289 , and nasal gDNA for @entity794 . RESULTS: The results show that prior to implant placement, all three bacterial species were below the lower limit of quantification in all edentulous @entity1 . The samples collected from the tongue and around the implants remained below the lower limit of quantification for each of the three species. However, all positive controls used in the study were detectable in the samples. qPCR standard curves showed correlation coefficients >0.97 and efficiencies >94.5% (slope range -3.19 to -3.46) for each of the SYBR green PCR assays. CONCLUSION: The results of this study indicate that the tested organisms did not emerge 6  months after implant placement irrespective of the nature of the implant biomaterial. A further follow-up of at least 2  years post-implantation of these @entity1 is suggested to determine whether there are any changes in the oral microbiota and whether such changes are associated with the development of @entity737 .
