Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional XXXX Cell Models.

@entity558 @entity137 @entity5 @entity281 @entity14
Traditional @entity5 treatments, such as chemotherapy and radiation therapy continue to have limited efficacy due to @entity5 . While bacterial @entity5 therapy has the potential to overcome this problem, it comes with the risk of @entity137 and @entity281 . To circumvent these issues, this paper investigates the anti- @entity5 effects of non-viable bacterial derivatives of Clostridium sporogenes. These non-viable derivatives are heat-inactivated C. sporogenes bacteria (IB) and the secreted bacterial proteins in culture media, known as @entity558 ( @entity558 ). In this project, the effects of IB and @entity558 on CT26 and HCT116 @entity14 cells were examined on a 2-Dimensional (2D) and 3-Dimensional (3D) platform. IB significantly inhibited cell proliferation of CT26 to 6.3% of the control in 72   hours for the 2D monolayer culture. In the 3D spheroid culture, cell proliferation of HCT116 spheroids notably dropped to 26.2%. Similarly the @entity558 also remarkably reduced the cell-proliferation of the CT26 cells to 2.4% and 20% in the 2D and 3D models, respectively. Interestingly the effect of boiled @entity558 (BCM) on the cells in the 3D model was less inhibitory than that of @entity558 . Thus, the inhibitive effect of inactivated C. sporogenes and its @entity558 on @entity14 cells is established.
