Validation of sixteen XXXX and @entity706 cell lines as controls for molecular gene rearrangement assays.

@entity588 @entity706 @entity18123 @entity6057 @entity1778 @entity1864
BACKGROUND: Assays for rearrangement of the immunoglobulin, T-cell receptor, @entity1864 , and @entity588 genes are valuable tools to aid in the diagnosis of @entity1778 and @entity706 and are now offered by many pathology laboratories. However, there is a lack of well-characterized and validated calibrators and positive controls for these assays. We therefore evaluated 16 readily available @entity1778 and @entity706 cell lines for their potential use as controls. METHODS: DNA and RNA were isolated from each cell line and analyzed by Southern blot and PCR or reverse transcription-PCR (RT-PCR). Rearrangements in the @entity18123 (H), @entity18123 (kappa), @entity6057 or TcR-gamma, @entity1864 , and @entity588 genes were detected by commercially available probes and primers. Cell lineages were confirmed by immunophenotyping. RESULTS: Immunoglobulin and T-cell receptor gene rearrangements were identified in five B- and three T-cell lines, respectively. Two cell lines tested positive for the @entity1864 gene, and one was positive for the @entity588 gene rearrangement by Southern blot. CONCLUSIONS: The 16 cell lines studied can be used as positive controls in molecular detection assays for gene rearrangements. The parallel processing of these cell lines with clinical samples can serve to quality control the experimental procedures from the first step of DNA or RNA extraction to the final step of result analysis.
