Apoptosis in ovarian granulosa cells of XXXX : Morphological features and clearance by homologous phagocytosis.

@entity3086 @entity28974 @entity1176 @entity1146 @entity291
UNASSIGNED: Apoptosis is involved in many physiological processes of the ovary, such as recruitment of prenatal germ cells, follicular @entity1146 , ovulation, and luteolysis. Based on the need for the involvement of phagocytic cells to achieve apoptosis clearance and that follicular @entity1146 is triggered by weak apoptotic stimuli, we postulate that granulosa cells engullng apoptotic corpses (ACs) must carry out this macrophagic process. Since apoptosis was early defined in terms of morphological aspects, here we describe apoptosis induced by a @entity1176 analog ( @entity3086 , LA) at histological level on @entity291 granulosa cells (primary culture, CPGB, and an established cell line, BGC-1). We observed two main types of apoptosis. In type A, the whole cell or most of it is compacted into a single large AC that is then engulfed by neighboring cells or simply detached. In type B, small portions of cells, either with or without nuclear material, become ACs that are also phagocytosed. Apoptosis and homologous phagocytosis were confirmed by TUNEL and immunocytochemistry for @entity28974 and active caspase 3. Induction of apoptosis was significant in BGC-1 cells treated for 24h with 100nM LA. CPGB cells showed two types of response with different doses of LA. Fetal calf serum was necessary to find apoptosis induced by LA.
