Abrogation of radiation-inducible telomerase upregulation in XXXX E6 transfectants of @entity1 lymphoblasts.

@entity1 @entity1885 @entity876 @entity1799 @entity2215
BACKGROUND: Telomerase activity in a @entity1 lymphoblastoid cell line with wild-type @entity876 status (TK6) was previously shown to be rapidly induced by ionizing radiation doses as low as 10 cGy. Since this low-dose response was absent in a closely related cell line overexpressing a mutant form of @entity876 (WTK1), the putative involvement of @entity876 was further investigated using stable @entity1799 ( @entity1799 ) E6 transfectants of these cell lines. The E6 product mediates rapid degradation of wild-type @entity876 , but has also been found to upregulate telomerase. MATERIAL AND METHODS: Telomerase activity in @entity1799 E6 transfectants of the @entity1 lymphoblastoid cell lines TK6 and WTK1 was measured by PCR/ELISA and was quantified using internal standards (titration by cell number) run within each separate assay. Mean telomere length was determined by Southern hybridization of terminal restriction fragments with a @entity1885 -labeled telomeric DNA probe. RESULTS: The TK6E6 and the WTK1E6 cells exhibited higher baseline telomerase activities than the parental cells. This was also accompanied by increased telomere lengths. Radiation exposure (up to 10 Gy) was unable to significantly further enhance telomerase activities, although the dynamic range of the assay would have allowed to record higher signals. CONCLUSION: The lacking radiation induction of telomerase activities in the E6 transfectants could reflect saturation, if E6 and radiation would share a common pathway of telomerase upregulation. Present evidence from the literature, however, suggests that E6 mediates @entity2215 ( @entity2215 ) subunit transcriptional activation, whereas radiation signals to posttranscriptional/posttranslational control of telomerase activity. Therefore, the present data enforce the previous hypothesis of a @entity876 dependence of telomerase upregulation by low doses of radiation and its abrogation, likely due to @entity876 degradation, in E6-expressing cells.
