The primary cilium undergoes dynamic size modifications during adipocyte differentiation of XXXX adipose stem cells.

@entity1 @entity28 @entity619 @entity74 @entity47635
UNASSIGNED: The primary cilium is an organelle present in most of the cells of the organism. Ciliopathies are @entity74 of the primary cilium and can be associated with @entity28 . We have studied the primary cilium during adipocyte differentiation of @entity1 adipose stem cells (hASC). We show here that the size of the primary cilium follows several modifications during adipocyte differentiation. It is absent in growing cells and appears in confluent cells. Interestingly, during the first days of differentiation, the cilium undergoes a dramatic elongation that can be mimicked by @entity619 alone. Thereafter, its size decreases. It can still be detected in cells that begin to accumulate lipids but is absent in cells that are filled with lipids. The cilium elongation does not seem to affect the localization of proteins associated with the cilium such as @entity47635 or Smoothened. However, Hedgehog signaling, an anti-adipogenic pathway dependent on the primary cilium, is inhibited after three days of differentiation, concomitantly with the cilium size increase. Together, these results shed new light on the primary cilium and could provide us with new information on adipocyte differentiation under normal and pathological conditions.
