[Changes of mitochondrial apoptosis in @entity2759 -reperfusion injury and the effects of Herba Erigerontis Breviscapi Injection preconditioning intervention in XXXX ].

@entity2759 @entity95 @entity63
OBJECTIVE: To investigate the mechanisms of mitochondrial apoptosis in @entity2759 -reperfusion injury and the effects of Herba Erigerontis Breviscapi Injection preconditioning intervention. METHODS: Sixty Japanese @entity95 were divided into sham-operated group, @entity63 group, @entity63 -reperfusion group (1, 6, 24 and 48 h), and Herba Erigerontis Breviscapi Injection group (1, 6, 24 and 48 h). Clamping the abdominal aorta was used to construct the @entity95 model of @entity2759 -reperfusion injury. The @entity95 in the @entity63 -reperfusion group and the Herba Erigerontis Breviscapi Injection group underwent reperfusion for 1, 6, 24, 48 h respectively after fifty-minute @entity63 . The @entity95 in the Herba Erigerontis Breviscapi Injection group were administered with Herba Erigerontis Breviscapi Injection at 9 mg/kg 30 minutes before @entity63 . Rate of apoptotic cells was measured by flow cytometry; contents of caspase-9 and apoptosis-inducing factor (AIF) in cytoplasm and serum were measured by enzyme-linked immunosorbent assay. RESULTS: Compared with the sham-operated group and the @entity63 group, the rates of apoptotic cells, the contents of caspase-9 and AIF in cytoplasm were increased at all time points after reperfusion, and the contents of caspase-9 and AIF in serum were decreased after 1 h and 6 h reperfusion, and increased after 24 h and 48 h reperfusion in the @entity63 -reperfusion group. Herba Erigerontis Breviscapi Injection intervention could decrease the rate of apoptotic cells and the contents of caspase-9 and AIF in cytoplasm and serum as compared with those in the @entity63 -reperfusion group, and the effects appeared after 1 h reperfusion. CONCLUSION: The apoptosis of nerve cells after @entity2759 -reperfusion is related to the mitochondrial pathways. Herba Erigerontis Breviscapi Injection can inhibit nerve cell apoptosis by decreasing the contents of caspase-9 and AIF in cytoplasm and serum.
