Injection of XXXX Cord Blood Cells With Hyaluronan Improves Postinfarction Cardiac Repair in @entity1707 .

@entity1 @entity32 @entity1707 @entity1188 @entity741 @entity299 @entity583
UNASSIGNED: : Although safe, recent clinical trials using autologous bone marrow or peripheral blood cells to treat @entity583 ( @entity583 ) show controversial results. These discrepancies are likely caused by factors such as aging, systemic @entity32 , and cell processing procedures, all of which might impair the regenerative capability of the cells used. Here, we tested whether injection of @entity1 cord blood mononuclear cells (CB-MNCs) combined with hyaluronan (HA) hydrogel improves cell therapy efficacy in a @entity1707 @entity583 model. A total of 34 minipigs were divided into 5 groups: sham operation (Sham), surgically induced- @entity583 plus injection with normal saline ( @entity583 +NS), HA only ( @entity583 +HA), CB-MNC only ( @entity583 +CB-MNC), or CB-MNC combined with HA ( @entity583 +CB-MNC/HA). Two months after the surgery, injection of @entity583 +CB-MNC/HA showed the highest left ventricle ejection fraction (51.32%    0.81%) compared with @entity583 +NS (42.87%    0.97%, p < .001), @entity583 +HA (44.2%    0.63%, p < .001), and @entity583 +CB-MNC (46.17%    0.39%, p < .001) groups. The hemodynamics data showed that @entity583 +CB-MNC/HA improved the systolic function (+dp/dt) and diastolic function (-dp/dt) as opposed to the other experimental groups, of which the CB-MNC alone group only modestly improved the systolic function (+dp/dt). In addition, CB-MNC alone or combined with HA injection significantly decreased the scar area and promoted angiogenesis in the infarcted region. Together, these results indicate that combined CB-MNC and HA treatment improves heart performance and may be a promising treatment for @entity741 . SIGNIFICANCE: This study using healthy @entity1 cord blood mononuclear cells (CB-MNCs) to treat @entity583 provides preclinical evidence that combined injection of hyaluronan and @entity1 CB-MNCs after @entity583 significantly increases cell retention in the peri- @entity1188 area, improves cardiac performance, and prevents @entity299 . Moreover, using healthy cells to replace dysfunctional autologous cells may constitute a better strategy to achieve heart repair and regeneration.
