Cell cycle progression of B-chronic lymphocytic leukemia cells induced to differentiate by XXXX .

@entity8574 @entity42427 @entity16079 @entity38770 @entity1072 @entity69 @entity665 @entity517 @entity1639 @entity865 @entity7527 @entity1199 @entity24957 @entity864 @entity2902
The cell cycle transition and differentiation-associated surface antigen expression was studied in a clone of @entity1072 ( @entity1072 ) with phenotypic properties similar to those of resting B lymphocytes. Differentiation was induced with @entity865 ( @entity864 ) and defined and quantitated by morphological and functional markers. Changes in the cell cycle position were determined by flow cytometry of @entity8574 orange-stained cells. The uninduced @entity1072 cells represented a homogeneous population with the same cell cycle position (GO) as resting normal peripheral blood lymphocytes. After five days of @entity865 stimulation, 56% of the @entity1072 cells were found in @entity16079 , 9% in G1B, and 3% in the S + G2/M phase, of which 2% was accounted to proliferating T cells. The cell cycle transition of the differentiating @entity1072 cells was also examined using cell cycle-associated surface antigens as markers. HLA-DR and @entity7527 antigens were present already on noninduced cells. The former had a high constant expression, while the amount of @entity7527 increased upon induction. The @entity38770 antigen was absent on noninduced cells but present on 86% of the induced cells. @entity24957 ( @entity42427 ) was expressed by the majority of the cells before @entity865 treatment and decreased to almost undetectable levels within 24 hours. Two antigens related to late stages of the cell cycle, the @entity1199 ( @entity1199 ; @entity2902 ) and the @entity69 receptor, were present on about 20% of the induced cells. Experiments with enriched T cells showed that T but not B cells incorporated @entity665 . Taken together these results and previous work on the induction of the protooncogene @entity517 and @entity1639 suggest that this @entity1072 clone represents GO cells that undergo differentiation without concomitant proliferation when exposed to @entity865 .
