Effects of @entity2847 on prolactin cellular @entity1184 , proliferation and secretory activity in @entity4742 -induced XXXX .

@entity2847 @entity5 @entity1184 @entity1373 @entity4743 @entity3695 @entity977 @entity35 @entity4742
@entity977 induced by chronic @entity4742 ( @entity4743 ) treatment in female F344 @entity35 were treated subsequently with @entity2847 (BC). Effects of BC on separable subpopulations of lactotrophs were examined. Enzymatically dissociated cells from individual pituitaries were assessed regarding total number, relative lactotroph population, intracellular prolactin ( @entity3695 ) content, @entity3695 release in primary culture, and density alterations by separation in Ficoll-Hypaque or after sedimentation at unit gravity. In addition to the treatment and analysis of in situ @entity5 , the effects of BC treatment in vitro were assessed, using @entity5 cells which were first separated on Ficoll-Hypaque. Cell proliferation was assessed by cell cycle analysis, using DNA measurement by laser flow cytometry. BC treatment of @entity5 reversed the effects of @entity4743 on pituitary weight, @entity3695 content and in vitro @entity3695 release. Total cell recovery was not affected by BC, but cell separation showed that BC reduced the number of larger @entity3695 -containing cells. Cell cycle analysis showed a decrease in numbers of cells in S and G2 cycle phases after BC in only one of four experiments. BC had an effect on proliferation in only the upper gradient fractions, containing the smallest cells. Culture of Ficoll-separated @entity5 cells revealed greater @entity3695 release among lighter/smaller cells. BC treatment inhibited @entity3695 release from both light and dense cells. The results establish that @entity3695 cell @entity1184 , as well as @entity1373 , results from @entity4743 treatment. @entity2847 treatment reverses this @entity1184 concomitant with inhibiting @entity3695 synthesis and release. Reversal of proliferation in @entity5 cells is not a major effect of @entity2847 treatment.
