Quantitation of @entity143 receptor NR2 mRNA transcripts in XXXX brain by competitive RT-PCR.

@entity8854 @entity1 @entity137 @entity535 @entity6806 @entity10731 @entity143
The @entity143 -selective ionotropic receptor constitutes one of the three principal classes of @entity8854 receptors within the @entity1 brain. It plays key roles in neuronal differentiation and synapse consolidation, activity-dependent forms of synaptic plasticity, and excitatory @entity535 -mediated @entity137 [Lab. Invest., 68 (1993) 372-387]. @entity143 receptors exist as multimeric complexes comprising proteins from two families, @entity6806 and NR2(A-D) [J. Biol. Chem., 271 (1996) 15669-15674]. Studies on recombinant receptors have revealed that while homomeric NR2 receptors are non-functional, co-expression of an @entity6806 with an NR2 subunit modulates the efficacy of the resulting channel [Nature, 357 (1992) 70-74]. The RT-PCR assay we describe here was developed to allow quantitation of all hNR2 transcripts in a single-tube PCR assay. Each hNR2 isoform is quantified on the basis of standard curves in which a known amount of synthetic @entity10731 competitor is co-amplified against total RNA. The protocol has been applied to the quantitation of hNR2 mRNA levels in autopsy brain. Used in conjunction with a method for the quantitation of @entity6806 transcripts [Brain Res. Protoc., in press], a complete analysis of @entity143 receptor mRNA expression can be obtained.
