The hepatoprotective effect of the combination use of Fructus Schisandrae with XXXX - a preclinical evaluation.

@entity407 @entity137 @entity1560 @entity28 @entity209 @entity2993 @entity2041 @entity412 @entity22519 @entity460 @entity382 @entity35 @entity6817 @entity165
ETHNOPHARMACOLOGICAL RELEVANCE: Fructus Schisandrae is traditionally used as a liver-toning Chinese herb. Recent studies suggested Fructus Schisandrae could prevent high-fat diet-induced @entity1560 as well as improving anti-oxidative status within the liver, which is a proposed mechanism against @entity6817 -induced liver @entity137 . AIM: The aim of the present study was to determine if the combination use of @entity2993 (AS) and Fructus Schisandrae aqueous extract (FSE) could (a) exert potent therapeutic effects not only on high-fat diet-induced @entity407 , but also on @entity382 (enlarge of liver size) and @entity1560 ( @entity1560 ); and (b) reduce side effects caused by intake of @entity6817 alone including increased incidence of elevated liver enzymes and liver @entity137 in @entity35 . MATERIALS AND METHODS: We studied 5 groups of @entity35 that were given the following treatment for 8 weeks: (i) Normal-chow diet; (ii) High-fat diet (contains 21% fat and 0.15% @entity165 ); (iii) High-fat diet (contains 21% fat and 0.15% @entity165 )+0.3% @entity2993 ; (iv) High-fat diet (contains 21% fat and 0.15% @entity165 )+0.45% FSE; (v) High-fat diet (contains 21% fat and 0.15% @entity165 )+0.3% @entity2993 +0.45% FSE. After 8 weeks of treatment, body weight, adipose tissue and liver mass were measured, and liver and plasma lipid levels were determined to evaluate to effect of FSE with or without AS treatment on diet-induced @entity28 , @entity407 and @entity1560 . Liver enzyme activities, anti-oxidative status and membrane permeability transition were also assessed to determine if FSE could reduce the side effects induced by AS. RESULTS: From the results, FSE treatment alone resulted in significant inhibitory effect on diet-induced increase in: a) body weight; b) fat pad mass (epididymal, perirenal and inguinal fat); c) liver weight; d) total liver lipid; e) liver @entity412 and @entity165 levels; and f) plasma lipid levels, suggesting FSE has a potential preventive beneficial effect on weight control and lipid metabolism in @entity35 with diet-induced @entity28 . However, FSE supplementation exerted no further beneficial effect on diet-induced @entity209 when it is combined with AS treatment, compared with @entity35 given AS-treatment alone. At the dose of 0.45%, dietary FSE supplementation resulted in: a) reduced liver enzymes (ALT and AST) levels; b) reduced macrophage infiltration ( @entity22519 ); c) improved liver @entity2041 levels (anti-oxidative status); d) reduced liver reactive oxidative species; e) a trend to reduce @entity460 -induced membrane permeability transition within the liver. Most importantly, these improvements induced by FSE treatment were not only observed in the livers of @entity35 given high-fat-diet, but also in high-fat-fed @entity35 with @entity2993 -induced hepatotoxicity. CONCLUSIONS: Taken together, these data suggested FSE has a potential beneficial effect on weight control and lipid metabolism in @entity35 with diet-induced @entity28 , and the combination use of FSE with AS could significantly prevent liver @entity137 and anti-oxidative status induced by AS alone.
