[Protective effects of @entity2493 in @entity647 XXXX caused by LPS].

@entity32 @entity2493 @entity35 @entity12220 @entity647
OBJECTIVE: To observe the expression of p- @entity12220 mitogen-activated protein kinase in lung tissues of @entity647 @entity35 model induced by lipopolysaccharide (LPS) and to explore the protective effects of @entity2493 (MT) in lung tissues in @entity35 . METHODS: Seventy-two @entity35 was randomly assigned to three groups, control group, LPS group and LPS + MT group. @entity35 model of ALI was established by instilling LPS intratracheally. We used immunohistochemical SP and Western blot method to detect the expression of p- @entity12220 mitogen-activated protein kinase in lung tissues and used light microscope to observe morphological changes. RESULTS: There were rare p- @entity12220 mitogen-activated protein kinase positive cells scattered in alveolar and airway epithelial cells in control group (P < 0.01). The positive p- @entity12220 mitogen-activated protein kinase cells in LPS group increased obviously than those in control group (P < 0.01), and were mainly distributed in infiltrative inflammatory cells, airway epithelial cells, alveolar epithelial cells and pleurames epithelial cells. In MT group, the p- @entity12220 mitogen-activated protein kinase positive cells in airway and lung tissues were much less than those in the LPS group (P < 0.05). The Western blot results were consistent with those of immunohistochemical method. CONCLUSION: The expression of p- @entity12220 mitogen-activated protein kinase increases in alveolar and airway epithelial cells in @entity647 @entity35 models induced by LPS. The activation of p- @entity12220 mitogen-activated protein kinase is found in most lung tissues, suggesting that p- @entity12220 mitogen-activated protein kinase participates in the signal transduction in inflammatory and noninflammatory cells. MT is an effective antioxidant, which relieves the @entity32 in @entity647 @entity35 , possibly through the inhibition of the pathway of @entity12220 MAPK over activation.
