Effects of acute heat stress on lipid metabolism of XXXX primary adipocytes.

@entity598 @entity8291 @entity8670 @entity3044 @entity6625 @entity291 @entity7443
UNASSIGNED: @entity8291 ( @entity8291 ) affects numerous physiological processes including nutrient partitioning and lipid metabolism. Objectives of this study were to evaluate how acute @entity8291 affects lipid metabolism in subcutaneous adipose tissue of dairy @entity291 . Adipose tissue biopsies were performed on Holstein @entity291 for @entity291 primary adipocyte isolation and cultured at either 42  C ( @entity8291 ) or 37  C (thermal neutral, @entity7443 ). Adipocytes were incubated with increasing @entity3044 ( @entity8670 ), and with increasing concentrations of insulin in the presence of @entity8670 to evaluate changes in lipolysis. Incorporation of radioactive @entity598 into lipids was measured as an indicator of lipogenesis. Abundance and phosphorylation of several lipolytic and lipogenic proteins were also measured. Adipocytes exposed to @entity8291 had an elevated maximal response to @entity8670 and were more sensitive to lipolytic stimulation by @entity8670 compared with cells cultured at @entity7443 . Thermal treatment did not affect the antilipolytic effects of insulin in the presence of @entity8670 . Lipogenesis measured as @entity598 incorporation was not altered by @entity8291 , but a temperature by insulin interaction was observed for the regulation of @entity6625 carboxylase, such that the presence of insulin resulted in a reduction in phosphorylation of @entity6625 carboxylase in adipocytes cultured at @entity7443 but not @entity8291 conditions. Results of this study demonstrate that acute @entity8291 has a direct effect on the regulation of lipolysis and the rate-limiting enzyme of lipogenesis in isolated @entity291 adipocytes.
