XXXX induces @entity137 and oxidative stress in @entity1 peripheral blood mononuclear cells.

@entity1 @entity137 @entity4508 @entity26 @entity2041 @entity6172 @entity98 @entity1234 @entity57066 @entity5139 @entity1865
UNASSIGNED: Previous studies have shown that @entity5139 pesticide (OP) exposure is associated with oxidative stress. @entity57066 ( @entity4508 ) is an OP widely used in agriculture, which is regarded as a highly toxic pesticide and it is a potent inhibitor of acetylcholinesterase. The aim of this study was to evaluate whether @entity4508 can induce oxidative stress at low concentrations in primary cultures of @entity1 peripheral blood mononuclear cells (PBMCs). PBMCs from healthy individuals were exposed to @entity4508 (0-80 mg/L) for 0-72 h. We performed the @entity98 and neutral-red assays to assess the @entity137 . As indicators of oxidative stress, the levels of reactive @entity26 species (ROS) were assessed using flow cytometry, and the @entity1234 ( @entity1865 ) and @entity2041 ( @entity6172 ) levels were determined. @entity4508 decreased the viability of PBMCs in a dose-dependent manner. At concentrations of 3, 10, or 20 mg/L for 24 h, @entity4508 increased the ROS production significantly compared with the vehicle control. Similarly, @entity4508 increased the levels of @entity1865 at the same concentrations that increased ROS (10 and 20 mg/L); however, no changes in @entity6172 levels were observed. These results suggest that @entity4508 increased the generation of oxidative stress in PBMCs.    2015 Wiley Periodicals, Inc. Environ Toxicol, 2015.
