Stimulation and inhibition of elastase release from XXXX neutrophil-dependence on the @entity460 messenger system.

@entity1 @entity11891 @entity1855 @entity4515 @entity6105 @entity11890 @entity460 @entity3365 @entity4514 @entity1098
The neutral proteinase elastase is released from polymorphonuclear (PMN) leukocytes in various physiological and pathological conditions. Aim of the present study was to gain further insight into the mechanisms which govern the liberation of this proteinase. Therefore, the effects of the @entity460 ionophore @entity3365 and of the protein kinase C activator @entity4514 ( @entity4515 ) on neutrophils were investigated in @entity1 whole-blood samples. Furthermore, the inhibitory effects of the @entity460 channel blocker @entity1855 and of the calmodulin blocker @entity6105 were followed. @entity3365 induced a release of elastase from neutrophils in a dose- and time-dependent manner. Complexation of extracellular @entity460 by @entity11890 ( @entity1098 ) completely abolished the stimulatory effect of @entity3365 . In a concentration of 10(-4) M @entity1855 was capable of attenuating (-49%) the @entity3365 -induced secretion of @entity11891 . Besides the increase in intracellular @entity460 concentration, the activation of protein kinase C by @entity4515 did also cause a release of neutrophil elastase. This release was strictly depending on the concentration of @entity4515 and the time of incubation. In contrast to the stimulatory effect of @entity3365 , the @entity4515 -induced liberation of neutrophil elastase was attenuated, but not completely abolished, by complexation of extracellular @entity460 with @entity1098 . Both 10(-4) M @entity1855 (-43%) and 10(-5) M @entity6105 (-42%) were able to reduce the @entity4515 -induced release of neutrophil elastase. Based upon these data, we conclude that both the translocation of @entity460 intracellularly by @entity3365 and the activation of protein kinase C by @entity4515 stimulate the release of neutrophil elastase. @entity1855 and @entity6105 were capable of suppressing the stimulation of elastase release.
