267 effect of seminal plasma in XXXX sperm.

@entity7857 @entity12312 @entity1319 @entity2667
In South American camelids, raw semen only presents sperm with oscillatory movements. Therefore, it is necessary to treat these cells to enable them to acquire progressive @entity1319 . The effects of raw seminal plasma (SP) on sperm movement patterns (oscillatory, progressive, and hyperactive) have apparently not yet been reported. The objective of this study was to determine effects of raw seminal plasma on sperm @entity1319 , viability, and acrosomal status in fresh @entity7857 semen. A total of 15 ejaculates were collected (electroejaculation) from 5 @entity7857 (n=5, r=3). Each ejaculate was diluted 4:1 in 0.1% collagenase in HEPES-TALP (HT) medium and incubated 4min at 37  C, with the objective of separating spermatozoa from SP. Immediately after incubation, each ejaculate was divided into 2 and centrifuged for 8min at 800*g. Pellets were resuspended in either HT or raw SP and maintained at 37  C until evaluation (at 0, 1.5, and 3h). Sperm @entity1319 was evaluated using a phase contrast microscope and a warm stage. @entity2667 and @entity12312 were used for assessing membrane integrity (viability). Acrosomal status was evaluated with the Coomassie blue stain. A split-plot design was used with treatment as a factor, with 2 levels (HT and SP) and time as the other factor, with 3 levels (0, 1.5, and 3h), and blocked by males. There was no significant interaction between treatments (HT and SP) and times (0, 1.5, and 3h) for each of the seminal characteristics evaluated. @entity1319 was observed after collagenase treatment in all samples. Progressive @entity1319 disappeared immediately after the addition of raw SP and showed only oscillatory movements. In contrast, samples incubated in HT maintained progressive @entity1319 and became hyperactive. There were no differences (P>0.05) in total @entity1319 of sperm incubated in HT among incubation times (0 h: 30.8  18.9%; 1.5 h: 26.5  11.5%; and 3h: 21.5  13.5%). However, in samples incubated with SP, a decrease (P<0.05) in @entity1319 was detected after 3h of incubation (0 h: 16.5  12.6%, 3 h: 2.3  3.2%). Sperm viability was not different (P>0.05) between treatments (HT and SP); samples incubated in HT retained 78.4% of the initial viability (32.8/41.8, 3 h/0h), and samples incubated in SP retained 69.7% of their initial viability (24.4/35.0, 3 h/0h). The percentage of spermatozoa with intact acrosomes was not different (P>0.05) between treatments (HT and SP); however, the percentage of sperm with intact acrosomes decreased after 3h of incubation in both samples (HT and SP). Due to the presence of a high percentage of progressive and hyperactive motile sperm in samples incubated in HT and their absence in samples incubated in SP, we concluded that raw seminal plasma preserved oscillatory sperm @entity1319 . Further studies are needed to understand the effects of SP on South American camelid spermatozoa.
