Real-time quantitative reverse transcriptase-polymerase chain reaction for luteinizing hormone-releasing hormone receptor gene mRNA expression in @entity1 XXXX .

@entity1 @entity4396 @entity263 @entity5 @entity1067
OBJECTIVES: To determine quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) detection of luteinizing hormone-releasing hormone ( @entity4396 ) receptor mRNA expressing @entity263 (CaP) and @entity1067 ( @entity1067 ) tissue by sequence-specific detection with hybridization probes using the LightCycler. Various in vitro studies have indicated that @entity4396 agonists and antagonists may have a direct inhibitory effect on CaP mediated by specific @entity4396 receptors. The incidence of @entity4396 receptor mRNA expression was demonstrated in 50% to 100% of clinical CaP and @entity1067 tissue by current RT-PCR methods. However, qualitative RT-PCR can only demonstrate the existence of a gene; an exact quantification of @entity4396 -receptor mRNA expression has not yet been done. METHODS: Quantitative real-time RT-PCR for @entity4396 receptor mRNA was performed using the LightCycler system and the RNA Amplification Kit hybridization probes in 35 @entity1 with CaP and 38 @entity1 with @entity1067 . RESULTS: Thirty-one (88.6%) of the 35 @entity1 with CaP and 36 (94.7%) of the 38 @entity1 with @entity1067 had positive RT-PCR for @entity4396 receptor mRNA. The number of positive amplifications per 1 microg total RNA was not significantly lower in CaP at 695,428 +/- 350,860 than in @entity1067 at 1,617,654 +/- 787,874. @entity1 with CaP evidenced a significant negative correlation between the amplification rate for @entity4396 receptor mRNA and Gleason score (r = -0.476; P = 0.004). CONCLUSIONS: In the past, the potential mechanisms acting on @entity4396 receptors in CaP have been identified as possible antitumor strategies for this type of @entity5 . Our study is the first to show that CaP does not have a higher expression of @entity4396 receptor mRNA expression than @entity1067 when using quantitative sequence-specific oligonucleotide hybridization probes that only detect certain PCR products.
