Cyclin-dependent kinase 1 gene expression is associated with poor prognosis in XXXX .

@entity1 @entity20127 @entity29331 @entity146 @entity5 @entity993 @entity189 @entity6826 @entity356
PURPOSE: A low expression level of cyclin-dependent kinase (Cdk) inhibitor @entity993 is associated with high @entity146 and poor prognosis of @entity189 . @entity1 @entity29331 ( @entity29331 ), as well as @entity20127 ( @entity20127 ), is an essential and specific factor in the @entity993 proteolysis by @entity6826 ( @entity20127 ) ubiquitin ligase. The purpose of this study is to clarify the clinical significance of @entity29331 expression and the relationship between @entity29331 and @entity993 expression in @entity356 . EXPERIMENTAL DESIGN: We measured @entity29331 expression using quantitative reverse transcription-PCR in 76 @entity1 @entity356 and @entity993 expression using immunohistochemistry in 28 cases. Moreover, we established @entity29331 - and/or @entity20127 -transfected @entity356 cell lines and assessed the relationship between @entity29331 , @entity20127 , and @entity993 expression using quantitative reverse transcription-PCR and Western blot analysis. RESULTS: @entity29331 high expression was correlated with poor prognosis (P < 0.05) and @entity29331 expression was inversely correlated with the expression level of @entity993 protein in @entity356 (P < 0.05). Using combined @entity20127 data [T-a. Masuda, @entity5 Res., 62: 3819-3825, 2002], 88.9% of the @entity29331 / @entity20127 double-high cases expressed a low level of @entity993 protein and showed the poorest prognosis (P < 0.05). Western blot analysis showed that @entity29331 / @entity20127 -cotransfected cells expressed a much lower level of @entity993 protein than the controls. CONCLUSIONS: These findings indicate that @entity29331 , as well as @entity20127 , regulates the expression level of @entity993 protein in @entity356 . @entity29331 could play an important role in @entity356 progression and would be a novel target for the treatment of @entity356 as well as a strong prognostic marker.
