@entity4896 (+) survivin(+) dendritic cell infiltration in dermal lesions of XXXX .

@entity1 @entity9846 @entity5750 @entity272 @entity4896 @entity785
INTRODUCTION: Proto-oncogene survivin is a member of the inhibitor of apoptosis (IAP) family of proteins. The presence of serous antibodies against survivin in @entity1 with @entity785 has been previously reported; however, there are few reports regarding the pathophysiological relationship between survivin and @entity785 . We herein investigated the expression and function of survivin in SSc @entity1 . METHODS: We performed immunohistochemistry analyses to determine the expression of @entity9846 , cIAP and survivin in @entity272 from @entity1 with SSc and non-SSc. The expression levels of survivin in peripheral blood mononuclear cells (PBMCs) obtained from SSc @entity1 and healthy controls were evaluated using RT-PCR and flow cytometry. Additionally, the function of survivin was verified with overexpression experiments using monocyte-derived dendritic cells (Mo-DCs). RESULTS: The expression patterns of both @entity9846 and cIAP were similar, while only the survivin expression differed between the SSc and non-SSc @entity272 . Survivin-overexpressing cells were detected in the SSc dermis frequently. The positive rate of survivin in SSc dermis (64.3  %, 9/14) was higher than that in non-SSc dermis (11.2  %, 1/9). Furthermore, survivin(+) cells expressed @entity4896 , one of the DC markers. Real-time PCR and FACS analyses revealed that the survivin-WT (wild type) expression levels in PBMCs, in particular @entity5750 (+) monocytes, from SSc @entity1 were higher than that from healthy controls. Additionally, the overexpression experiments showed that survivin-WT-overexpressing @entity4896 (+) Mo-DCs have the characteristics of promoting cell cycle progression and decreasing apoptotic cells. CONCLUSIONS: These findings suggest that dermal survivin(+) @entity4896 (+) cell infiltration may be a potential biomarker of SSc @entity272 . PBMCs and monocytes from SSc @entity1 also overexpressed survivin; therefore, dermal survivin(+) DC may be derived from peripheral blood monocytes. Additionally, survivin may be involved in dermal @entity4896 (+) DC proliferation through cell cycle activation and resistance to apoptosis. Survivin may be an important molecule for the pathogenesis of SSc.
