[Quick determination of 39 hormones residues in XXXX formula by liquid chromatography-tandem mass spectrometry].

@entity172 @entity1 @entity4315 @entity3265 @entity1042 @entity1626 @entity6173 @entity3440 @entity15 @entity3800 @entity5189
A quick confirmative method was developed for determining the residues of 17 glucocorticoids, 11 progesterones, 3 @entity3800 and 8 @entity172 in @entity1 formula by liquid chromatography-tandem quadrupole mass spectrometry (LC- @entity15 / @entity15 ). The sample was extracted with @entity1626 at first. Then the lipid substances were removed by centrifugation under freezing condition and liquid-liquid extraction with @entity4315 of the extract. The purification was carried out on hydrophilic-lipophilic solid-phase extraction columns and @entity1042 was used as the eluted solvent. The detection of 39 analytes was carried out in the positive or negative multi-reaction monitoring (MRM) mode, separately. Acetonitrile-0.1% @entity5189 was used as the mobile phase and an ordinary @entity3440 gel @entity6173 column was selected to separate the analytes in the positive mode. @entity1626 -0.1% aqueous @entity3265 as mobile phase and the separation was carried out on an ultra-performance @entity6173 column with a wide pH range in the negative mode. The limits of quantification (LOQ, S/N > or = 10) were 0.02-5 microg/kg. The overall recoveries varied from 59.5% to 117.9%, and the relative standard deviations (RSD) were between 6.4% and 16.3%. The real sample tests showed that the simple and accurate method can be used for determining the residues of multi-endogenous and chemically synthesized hormones in milk powders.
