Improving the comparative map of XXXX -q13 by sample sequencing of BAC clones.

@entity1 @entity1707 @entity8824 @entity377 @entity35 @entity121
To improve the comparative map for @entity1707 chromosome 2 and increase the gene density on this chromosome, a porcine bacterial artificial chromosome (BAC) library was screened with 17 microsatellite markers and 18 genes previously assigned to @entity1707 chromosome 2. Fifty-one BAC clones located in the region of a maternally imprinted quantitative trait locus for @entity121 ( @entity121 ) were identified. From these BACs 372 kb were sample sequenced. The average read length of a subclone was 442 basepair (bp). Contig assembly analysis showed that every bp was sequenced 1.28 times. Subsequently, sequences were compared with sequences in the @entity377 databases to identify homology with other @entity1 sequences. Sequence identity was observed with sequences derived from 35 BACs. The average percentage identity with @entity1 sequences was 87.6%, with an average length of 143 bp. In total, sample sequencing of all BACs resulted in sequence identity with 29 @entity1 genes, 13 @entity1 expressed sequence tags (ESTs), 17 @entity1 genomic clones, one @entity35 gene, one porcine gene and nine porcine ESTs. Eighteen genes located on @entity1 chromosome 11 and 19, and seven genes from other @entity1 locations, one @entity35 gene and one porcine gene were assigned to @entity1707 chromosome 2 for the first time. The new genes were added to the radiation hybrid map at the same position as the locus from which the BAC that was sequenced was derived. In total 57 genes were placed on the radiation hybrid map of @entity8824 -q13.
