Progestin regulates chemokine (C-X-C motif) ligand 14 transcript level in XXXX endometrium.

@entity1 @entity134 @entity15874 @entity1420
Leukocyte populations change profoundly in the @entity1 endometrium during the menstrual cycle. However the predominant cell, the uterine natural killer (uNK) cell does not contain @entity134 receptors. From gene array analysis we identified a transcript encoding chemokine (C-X-C motif) ligand 14 ( @entity15874 ) which is markedly up-regulated in the secretory phase of the cycle. We confirm this data by northern blotting and quantitative PCR. Using in situ hybridization we localized @entity15874 mRNA to the glandular epithelial cells where it was detected only in the secretory phase of the cycle. Candidate @entity1420 response elements were identified at positions -2028/-2007 and -722/-697 (PRE1 and PRE2, respectively) relative to the translation start site. These were functionally tested using luciferase reporter deletion constructs, electrophoretic mobility shift assays and site-directed mutagenesis. The deletion/mutation of these sites reduced @entity1420 induction by 40 and 20%, respectively. Finally, we demonstrated that recombinant @entity15874 stimulated uNK cell chemotaxis in vitro. We therefore conclude that @entity15874 is likely to be regulated by @entity1420 in @entity1 endometrium and that it may exert a chemoattractive effect on uNK cells and in part be responsible for their clustering around the epithelial glands.
