Hemodynamic improvement and removal of autoantibodies against beta1-adrenergic receptor by immunoadsorption therapy in XXXX .

@entity1 @entity384 @entity2161
The removal of @entity2161 ) autoantibodies by immunoadsorption (IA) has been proposed as a potential mechanism for the improvement of the left ventricular function in @entity384 ( @entity384 ). In the present study, the possible association between removal of the autoantibodies against the @entity1 @entity2161 with the hemodynamic improvement induced by IA was investigated.IA was performed in 22 @entity384 @entity1 (n=22; NYHA III-IV, EF<30%, stable medication). The @entity2161 autoantibodies from column eluents (CE) were detected by enzyme-linked immunosorbent assay (ELISA) and BIAcore methods. CE of 32% (7/22) of the @entity1 was found to be antibody-positive with ELISA or BIAcore. In addition, a bioassay system was also used for the detection of this autoantibody. Seventy-three percent (16/22) of the @entity1 were found to be antibody-positive by this method. However, independent of the @entity2161 antibody detection method, both antibody-positive and antibody-negative groups showed similar acute and prolonged hemodynamic improvements during IA therapy. Furthermore, antibody-positive and -negative groups received a comparable improvement of left ventricular ejection fraction. These results suggest that different mechanisms are involved in the hemodynamic improvement induced by IA. The beneficial hemodynamic effects induced by IA are not directly associated with the removal of @entity2161 autoantibodies.
