In vivo @entity2227 neurotoxicity is associated with reductions in XXXX /calmodulin-dependent protein kinase II immunoreactivity.

@entity8854 @entity1400 @entity2227 @entity1399 @entity7464 @entity460 @entity27303 @entity204
@entity460 /calmodulin-dependent protein kinase II (CaM kinase) activity is inhibited in cultured hippocampal cells following direct application of @entity2227 . The goal of the present study was to determine if hippocampal regions that undergo delayed cell @entity204 following @entity2227 microinfusion would exhibit changes in CaM kinase immunoreactivity. Gerbils received bilateral intra-hippocampal infusions of @entity8854 (34 microg/microl), or control treatments of @entity27303 or saline. Animals were sacrificed at 12 or 24 hr to assess cell loss and determine changes in CaM kinase-like immunoreactivity. Hippocampi of gerbils euthanized 12 hr following @entity8854 , or 24 hr following @entity27303 , did not exhibit cell @entity204 in the hippocampal @entity1399 region. Animals injected with @entity8854 and sacrificed 24 hr after infusion had extensive cell damage that was restricted to the hippocampal @entity1399 region. CaM kinase-like immunoreactivity was absent in the hippocampal @entity1399 region of all @entity8854 treated animals sacrificed at 12 hr. In these same sections, CaM kinase immunoreactivity was evident in the subiculum, @entity7464 and @entity1400 regions. Reduction in CaM kinase immunoreactivity following @entity8854 were also observed using Western analysis. The results confirm and extend the findings of earlier cell culture studies by demonstrating a reduction in CaM kinase immunoreactivity that occurred prior to cell @entity204 .
