Growth factors and their receptors in the @entity2172 during XXXX media.

@entity2172 @entity10816 @entity327 @entity11943 @entity33 @entity281 @entity35 @entity2346
OBJECTIVE: The hyperplastic response of the @entity2172 during bacterial @entity2346 media is thought to be mediated by the actions of growth factors and their respective receptors. The purpose of the study was to explore the expression of growth factors known to stimulate epithelial cells in other systems, as well as their receptors, in the @entity2172 during @entity2346 media. STUDY DESIGN: Expression of mRNA growth factors and receptors was measured over time after inoculation of the @entity35 middle ear with bacteria. METHODS: The middle ears of 12 male @entity35 were injected with 10(5)/mL @entity327 3655 (nontypeable, biotype II). Three @entity35 were killed at 6, 24, 48, and 72 hours. Three untreated @entity35 were also killed to serve as negative controls. The @entity2172 samples were surgically removed and homogenized. Reverse transcription-polymerase chain reaction was performed on each sample with primers for @entity35 epidermal growth factor, @entity10816 (ErbB), heparin binding epidermal-like growth factor, hepatocyte growth factor, hepatocyte growth factor receptor, @entity11943 , betacellulin, amphiregulin, and neuregulin-alpha. RESULTS: Hepatocyte growth factor and @entity10816 primers demonstrated polymerase chain reaction products of the expected size that were not displayed in the normal @entity2172 . @entity11943 and hepatocyte growth factor receptor demonstrated polymerase chain reaction products at all time points tested. Betacellulin and neuregulin-alpha products were present at all time points except 72 hours after @entity281 . CONCLUSIONS: The results of the study support a role for growth factors in the @entity2172 during @entity2346 media. These bioactive ingredients contribute to @entity33 .
