In ovo administration of human recombinant leptin shows dose dependent angiogenic effect on XXXX chorioallantoic membrane.

@entity231 @entity47003 @entity70664 @entity63 @entity1711 @entity1573 @entity1655 @entity67752 @entity6175
BACKGROUND: @entity67752 , the cytokine produced by white adipose tissue is known to regulate food energy homeostasis through its hypothalamic receptor. In vitro studies have demonstrated that @entity67752 plays a major role in angiogenesis through binding to the receptor Ob-R present on ECs by stimulating and initiating new capillary like structures from ECs. Various in vivo studies indicate that @entity67752 has diverse effect on angiogenesis. A few reports have showed that @entity67752 exerts pro angiogenic effects while some suggested that it has antiangiogenic potential. It is theoretically highly important to understand the effect of @entity67752 on angiogenesis to use as a therapeutic molecule in various angiogenesis related pathological conditions. @entity1573 @entity231 ( @entity231 ) on 9th day of incubation was incubated with 1, 3 and 5   g concentration of HRL for 72  h using gelatin sponge. Images where taken after every 24  h of incubation and analysed with Angioguant software. The treated area was observed under microscope and histological evaluation was performed for the same. Tissue thickness was calculated morphometrically from @entity6175 and @entity1711 stained cross sections. Reverse transcriptase PCR and immunohistochemistry were also performed to study the gene and protein level expression of angiogenic molecules. RESULTS: HRL has the ability to induce new vessel formation at the treated area and growth of the newly formed vessels and cellular morphological changes occur in a dose dependent manner. Increase in the tissue thickness at the treated area is suggestive of initiation of new capillary like structures. Elevated mRNA and protein level expression of @entity1655 and @entity47003 along with the activation of ECs as demonstrated by the presence of @entity70664 expression supports the neovascularization potential of HRL. CONCLUSION: Angiogenic potential of HRL depends on the concentration and time of incubation and is involved in the activation of ECs along with the major interaction of @entity1655 and @entity47003 . It is also observed that 3   g of HRL exhibits maximum angiogenic potential at 72  h of incubation. Thus our data suggest that dose dependent angiogenic potential HRL could provide a novel role in angiogenic dependent therapeutics such as @entity63 and wound healing conditions.
