@entity1255 and XXXX as main determinants of oxidative stress in the elderly.

@entity1 @entity32 @entity2002 @entity1357 @entity558 @entity1255 @entity52546 @entity1674 @entity2186 @entity3563 @entity1110 @entity165 @entity26457 @entity224 @entity12487
Oxidative stress is commonly observed in the elderly and could be involved in age-related diseases. However, the determinants of @entity224 anion overproduction are not clearly understood. @entity224 anion production was evaluated using a @entity12487 -based chemiluminescence method in 478 elderly subjects (304 @entity1 , 174 @entity1 ; 79.5+/-7.1 years). @entity1255 (HCy) metabolism ( @entity3563 , @entity2186 , plasma, and erythrocyte @entity26457 ), @entity32 (CRP, @entity2002 , alpha-1 acid glycoprotein), lipid parameters (total @entity165 , @entity1674 , HDL and LDL @entity165 ), and nutritional parameters (albumin, transthyretin) were determined. The results show that HCy levels (p<0.001) and @entity224 anion production (p=0.04) increase with aging, but CRP does not. Highest HCy (>20 microM) (OR 1.83 (1.09-3.07), p=0.02) and CRP over 5 mg/L (adjusted OR 2.01 (1.15-3.51), p=0.01) are the main determinants in @entity224 anion production in the elderly. These clinical data are confirmed in an in vitro study using @entity1357 monocyte-like cells. Incubation with HCy @entity52546 (HTL) (0-200 microM) and @entity558 (0-20 ng/ml) dramatically enhances @entity1110 oxidase expression and activation. Moreover, a synergic action was evidenced for low concentrations of HTL (20 microM) and @entity558 (5 ng). Taken together, the clinical data and in vitro experiments support the hypothesis that moderate @entity3563 and low-grade @entity32 synergically enhance @entity1110 oxidase activity in the elderly.
