Adiponectin accumulates in myocardial tissue that has been damaged by XXXX -reperfusion injury via leakage from the vascular compartment.

@entity19 @entity2806 @entity741 @entity63
OBJECTIVES: Adiponectin, a circulating adipocyte-derived hormone, exerts beneficial actions on hearts subjected to @entity63 -reperfusion injury. Adiponectin exists in plasma as three different oligomeric forms: trimer, hexamer and high molecular weight. This study investigated the expression and myocardial accumulation of adiponectin in a @entity19 model of @entity63 -reperfusion injury. METHODS: Wild-type and adiponectin deficient @entity19 were subjected to left anterior descending artery ligation followed by reperfusion. Plasma adiponectin levels were analyzed by ELISA and adiponectin in heart was determined by immunohistochemical, Western blot and real-time PCR analyses. RESULTS: Plasma adiponectin levels declined after @entity741 -reperfusion injury due to reductions in high molecular weight and, to a lesser extent, trimer and hexamer isoforms. Adiponectin protein was detected in injured but not sham-operated heart, and this was accompanied by a negligible increase in adiponectin transcript in the myocardium. Systemic delivery of adiponectin to adiponectin knockout (APN-KO) @entity19 led to the accumulation of adiponectin in @entity63 -reperfusion-injured, but not-uninjured hearts at levels comparable to wild-type suggesting that cardiac expression of adiponectin does not appreciably contribute to its accumulation in the infarcted heart. The serum half-life of adiponectin was 7.4+/-0.3 h in @entity63 -reperfusion and 9.7+/-0.5 h in sham-operated @entity19 (P>0.05), whereas the half-life of adiponectin in the damaged heart was 26.9+/-2.2 h (P<0.05). CONCLUSIONS: These data show that adiponectin accumulates in the heart following @entity2806 primarily through leakage from the vascular compartment, and that adiponectin has a longer half-life in damaged heart tissue than in plasma.
