Quantitative abnormalities of fetal trophoblast cells in maternal circulation in XXXX .

@entity1 @entity7955 @entity16438 @entity1468 @entity6393
OBJECTIVE: To determine if quantitative abnormalities of circulating fetal trophoblast cells (CFTCs) are associated with @entity1468 . METHODS: The trophoblast cell-specific antibody, MEM-G/9 (monoclonal antibody to @entity6393 ), was applied to distinguish the trophoblast cells from the maternal circulation. The trophoblast cells were isolated by density-gradient centrifugation from maternal blood samples of normal pregnant and preeclamptic @entity1 , respectively. After preliminary enrichment, the CFTCs were identified by immunocytochemical staining with the MEM-G/9. To prove fetal origin of the @entity6393 -positive cells, primer-extension preamplification (PEP) and polymerase chain reaction (PCR) based on single @entity6393 -positive cells were adopted to detect @entity1 @entity16438 gene. RESULTS: There were 6.88 +/- 1.54 and 30.56 +/- 5.16 @entity6393 -positive cells in 6 mL maternal blood from the normal pregnant (n = 16) and preeclamptic @entity1 (n = 18), respectively. The difference was statistically significant (p < 0.001). The @entity16438 gene from the @entity6393 -positive cells was detected in all pregnant @entity1 carrying male fetuses. The sensitivity and specificity of @entity7955 and PCR for the @entity16438 gene detection were 100%. CONCLUSION: It is concluded that enhancement of CFTCs numbers is related to @entity1468 , which provides information useful for noninvasive prenatal diagnosis of @entity1468 .
