Alternate activation of two divergently transcribed XXXX genes from a bidirectional promoter is linked to changes in histone modification.

@entity1 @entity2047 @entity19 @entity2340 @entity3996 @entity10591 @entity1014 @entity10594
@entity1014 kinase (TK) is a growth factor-inducible enzyme that is highly expressed in proliferating @entity1 cells. Expression of @entity19 TK mRNA is controlled by transcriptional and posttranscriptional mechanisms including antisense transcription. Here we report the identification of a novel gene that is divergently transcribed from the bidirectional TK promoter. This gene encodes @entity10594 formamidase (KF), an enzyme of the @entity2340 metabolism. Whereas the TK gene is induced upon @entity2047 -mediated activation of resting T cells, the KF gene becomes simultaneously repressed. The TK promoter is regulated by E2F, @entity10591 , histone acetyltransferases, and deacetylases. The binding site for the growth-regulated transcription factor E2F is beneficial for TK promoter activity but not required for KF expression. In contrast, the @entity10591 binding site is crucial for transcription in both directions. Inhibition of histone deacetylases by @entity3996 leads to increased histone acetylation at the TK/KF promoter and thereby to selective activation of the TK promoter and simultaneous shut-off of KF expression. Similarly, TK gene activation by @entity2047 is linked to histone hyperacetylation, whereas KF expression correlates with reduced histone acetylation. The KF gene is the rare example of a @entity1 gene whose expression is linked to histone hypoacetylation at its promoter.
