MicroRNA-29b is a Novel Prognostic Marker in XXXX .

@entity1 @entity14107 @entity5 @entity4404 @entity695 @entity2423 @entity14 @entity15702 @entity3
PURPOSE: Recent studies have suggested that @entity695 ( @entity695 ) family members may play important roles in @entity1 @entity5 by regulating cell proliferation, differentiation, apoptosis, migration, and invasion. The present study aimed to investigate the clinical significance and biological function of @entity15702 in @entity14 ( @entity14 ). METHODS: Real-time polymerase chain reaction was used to quantify @entity15702 expression. The association between @entity15702 and survival was evaluated in 245 @entity1 with @entity14 . We transfected an @entity15702 mimetic into @entity14 cells to explore the functional role of @entity15702 in vitro, based on a proliferation assay, flow cytometry, and Western blotting. RESULTS: In clinical samples of @entity14 , @entity15702 expression was significantly reduced in @entity5 tissues compared with normal mucosa (p  <  0.012). Multivariate survival analyses indicated that @entity15702 expression was an independent prognostic factor for disease-free survival (p  =  0.026), lymph node @entity3 (p  =  0.004), and pathological T classification (p  =  0.002). In a multivariate analysis of 5-year overall survival, we found a similar association between lymph node @entity3 , pathological T classification, venous invasion, and @entity15702 expression (p  =  0.013). In vitro, low Ki-67-positive staining showed that administration of the mimic- @entity15702 reduced proliferation of @entity14 cells. An @entity4404 apoptosis assay and flow cytometric analysis revealed that @entity15702 induced apoptosis and arrested the cell cycle at the G1/S transition. Moreover, @entity15702 inhibited the expression of @entity2423 and @entity14107 . CONCLUSIONS: Our findings indicated that @entity15702 may be a useful, novel, prognostic marker and may play important roles in regulating apoptosis and cell cycle in @entity14 .
