arrow-leafed lu @entity16413 Lupinus angustifolius L.) b-conglutin proteins modulate the insulin signalling pathway as potential type 2 di XXXX reatment and inflammatory-related disease amelioration.

@entity2175 @entity1 @entity10175 @entity6 @entity1356 @entity584 @entity2368 @entity15237 @entity46171 @entity16413 @entity8569
SCOPE: We have investigated the potential use of b-conglutin protein isoforms from narrow-leafed @entity16413 (Lupinus angustifolius L.) as a @entity6 treatment. METHODS AND RESULTS: We produced purified recombinant b1-, b2-, b3-, b4-, and b6-conglutin proteins and showed that @entity46171 could bind to insulin. To assess b-conglutin proteins modulatory effect on insulin-activation meditated kinases, whole blood and peripheral blood mononuclear cell (PBMC) cultures from @entity2175 ( @entity2175 ) and healthy control subjects (C) were incubated with conglutin proteins. Treatment of PBMCs from @entity2175 @entity1 with b1, b3, and b6 proteins increased up to 3-folds mRNA and protein levels of genes important in insulin signalling pathways, namely @entity10175 / @entity8569 / @entity584 / @entity2368 . This was accompanied by a comparable fold-change decrease in the mRNA expression level of pro-inflammatory genes ( @entity15237 and @entity1356 ) and proteins compared to healthy controls. The b2 and b4 isoforms had no effect on the insulin signalling pathway. However, these b-conglutin proteins elicited pro-inflammatory effects since levels of mRNA and proteins of @entity15237 and @entity1356 were increased. CONCLUSION: Our results raise the possibility of using these particular b-conglutin proteins in the prevention and treatment of @entity6 , as well as their potential as anti-inflammatory molecules. This article is protected by copyright. All rights reserved.
