Coexpressed @entity842 synthase and apical XXXX integrins influence tubule cell adhesion after cytokine-induced injury.

@entity1 @entity6708 @entity295 @entity10483 @entity66 @entity39 @entity5 @entity63 @entity5962 @entity842 @entity1192 @entity810 @entity379 @entity6554 @entity551 @entity130 @entity3623
In @entity295 -induced @entity810 , actin cytoskeletal alterations result in shedding of proximal tubule epithelial cells (PTEC) and @entity6554 . This study examined the hypothesis that inflammatory cytokines, released early in @entity295 , cause PTEC cytoskeletal damage and alter integrin-dependent cell-matrix adhesion. The question of whether the intermediate @entity842 (NO) modulates these cytokine effects was also examined. After exposure of @entity1 PTEC to @entity5 @entity39 factor-alpha, @entity1192 , and @entity379 , the actin cytoskeleton was disrupted and cells became elongated, with extension of long filopodial processes. Cytokines induced shedding of viable, apoptotic, and necrotic PTEC, which was dependent on NO synthesized by @entity3623 ( @entity3623 ) produced as a result of cytokine actions on PTEC. Basolateral exposure of polarized PTEC monolayers to cytokines induced maximal NO-dependent cell shedding, mediated in part through NO effects on @entity6708 . Cell shedding was accompanied by dispersal of basolateral @entity10483 integrins and @entity5962 , with corresponding upregulation of integrin expression in clusters of cells elevated above the epithelial monolayer. These cells demonstrated coexpression of @entity3623 and apically redistributed @entity10483 integrins. Attachment studies demonstrated that the major ligand involved in cell anchorage was laminin, probably through interactions with the integrin alpha(3) @entity10483 . This interaction was downregulated by cytokines but was not dependent on NO. These studies provide a mechanism by which inflammatory cytokines induce @entity66 in @entity295 , in the absence of @entity551 and @entity63 . Future therapeutic strategies aimed at specific @entity3623 inhibition might inhibit PTEC shedding after cytokine-induced @entity130 of @entity810 in @entity295 .
