Modeling and Integral X-Ray, Optical, and MRI Visualization of Multiorgan Metastases of Orthotopic 4T1 XXXX in BALB/c @entity19 .

@entity1 @entity0 @entity19 @entity5 @entity3 @entity11432
UNASSIGNED: A model of highly metastasizing @entity0 was reproduced and standardized in experiments on BALB/c @entity19 . 4T1 cells characterized by high metastatic activity were transfected with @entity11432 ( @entity11432 ) gene or firefly luciferase (Luc2) gene. Unmodified 4T1 cells and modified 4T1- @entity11432 and 4T1-Luc2 cells were subcutaneously injected to mature female @entity19 into the second mammary fat pads. Quantitative evaluation of the primary node and visceral @entity3 was performed using magnetic-resonance imaging, X-ray and optical tomography. Modification of 4T1 cells with @entity11432 gene considerably reduced their invasive and metastatic potential and led to spontaneous regression of the @entity5 in 20% cases. Modification of 4T1 cells with Luc2 gene had practically no effect on proliferative, invasive, and metastatic characteristics of the @entity5 and provided the possibility of quantitative analysis of the @entity5 dynamics by the luminescence intensity. The survival median in @entity19 receiving unmodified 4T1 cells and transfected 4T1- @entity11432 and 4  1-Luc2 cells was 32, 42, and 38 days, respectively. Neither primary node nor @entity5 @entity3 accumulated gadolinium-containing contrast agent and Alasens fluorescent tracer. After implantation of 4T1 and 4  1-Luc2 cells, multiple @entity3 were more often detected in the lungs, liver, spleen, spine, and regional lymph nodes and less frequently in the brain, which corresponded to metastasizing profile of @entity1 @entity0 . The developed model of @entity0 4T1 in BALB/c @entity19 with complex detection of multiple organ @entity3 using X-ray microCT, optical, and MRI can be recommended for preclinical studies of new antitumor preparations.
