Morphological analysis on adhesion and invasion involved in XXXX with tissue culture.

@entity1 @entity43 @entity10585 @entity2282 @entity42 @entity46
BACKGROUND: Endometriosis (EM) is a benign gynecologic disease predominantly found in @entity1 of reproductive age. However, its pathogenesis is still poorly understood. Our experiment was designed to establish a stable and reliable cultural environment for coculture of endometrium and peritoneum, so as to observe the adhesion/invasion ability of endometrium from @entity1 with or without EM. METHODS: Endometria of secretory phase and peritoneum were sampled from 6 @entity1 with endometriois during laparoscopy. Six with @entity43 or simple @entity42 were taken as control. We cocultured endometrium and peritoneum into four groups (endometrium from EM cultured with peritoneum from EM, endometrium from control cultured with peritoneum from control, endometrium from EM cultured with peritoneum from non-EM and the endometrium from control cultured with peritoneum from EM) to observe the adhesion/invasion process in gas-liquid surface culture and in-medium culture. Specimens were collected at 1 hour, 6 hours, 12 hours, 24 hours, 2 days, 3 days, 4 days, 5 days, 6 days and 7 days for histology, immunofluorescence and immunohistochemical analysis on @entity10585 ( @entity10585 ) and @entity2282 . RESULTS: The gas-liquid surface culture was superior to in-medium culture for the maintenance of tissue morphology and survival of endometrium. @entity10585 immunoflurescence demonstrated no remarkable difference in adhesion process between @entity1 with and without EM. @entity2282 immunochemistry manifested frequent invasion of endometrial stromal cells from EM @entity1 into peritoneum of up to 3 days culture, while the endometriotic cells from non-EM @entity1 did not invade into peritoneum. CONCLUSIONS: Gas-liquid surface culture is a suitable model for observing the early events in EM lesion formation. Endometrium from @entity1 with EM showed increased invasion capacity during coculture, which might help to explain the etiology of @entity46 .
