Sublethal Photothermal Stimulation with a Micropulse Laser Induces Heat Shock Protein Expression in XXXX Cells.

@entity1 @entity2285 @entity1103 @entity989
Purpose/Aim of the Study. Subthreshold micropulse diode laser photocoagulation is an effective treatment for @entity1103 . The molecular mechanisms underlying treatment success are poorly understood. Therefore, we investigated the effects of sublethal laser energy doses on a single layer of densely cultured @entity1 cells as a model of the @entity1 @entity989 ( @entity989 ). Materials and Methods. A single layer of densely cultured @entity1 @entity1 cells was perpendicularly irradiated with a micropulse diode laser. Nonirradiated cells served as controls. Sublethal laser energy was applied to form a photocoagulation-like area in the cultured cell layers. @entity2285 expression was evaluated using quantitative polymerase chain reaction and immunocytochemistry. Results. Photocoagulation-like areas were successfully created in cultured @entity1 cell layers using sublethal laser energy with our laser irradiation system. @entity2285 mRNA expression in cell layers was induced within 30   min of laser irradiation, peaking at 3   h after irradiation. This increase was dependent on the number of laser pulses. @entity2285 upregulation was not observed in untreated cell layers. Immunostaining indicated that @entity2285 expression occurred concentrically around laser irradiation sites and persisted for 24   h following irradiation. Conclusion. Sublethal photothermal stimulation with a micropulse laser may facilitate @entity2285 expression in the @entity989 without inducing cellular damage.
