XXXX mutation is a rare event in Merkel cell carcinoma of the head and neck.

@entity1 @entity3852 @entity1522 @entity146 @entity5 @entity876 @entity72457
The aim of this retrospective analysis was to evaluate the status of @entity876 and possible mutations in @entity3852 ( @entity3852 ) cell lines and @entity3852 tissue samples. The @entity876 mutations are common in different @entity5 origins but rare in MCCs detected so far. MCCs are highly aggressive @entity5 with an enhanced potential to metastasize. Until now, less is known about @entity3852 and new approaches to understand this disease are necessary. RNA and DNA were extracted from two @entity3852 cell lines and 27 archival paraffin-embedded @entity1 samples. After reverse transcription, a real-time PCR and a high-resolution melt analysis were carried out. In both @entity3852 cell lines, we could detect a @entity876 missense mutation at codon 193 (exon 6) with a change in @entity1522 ( @entity72457 ). This mutation was equal in both cell lines and was investigated in 27 tissue samples in succession to detect possible accounts for the aggressive behavior of MCCs. Unfortunately, no corresponding @entity876 mutation could be observed in the investigated tissue samples. A new @entity876 mutation was detected in @entity3852 cell lines. This mutation could not be determined in @entity1 ' samples. Therefore, the @entity146 of @entity3852 seems to be independent of @entity876 mutations and other mutations might be responsible for developing @entity3852 .
