A sensitive XXXX immunoassay for pharmacokinetic and distribution studies.

@entity1 @entity1472 @entity2379 @entity19 @entity95 @entity11015
Since currently used assays of @entity2379 ( @entity11015 ) lack sensitivity or are not easily adaptable in all laboratories, we have developed an enzyme immunoassay for @entity11015 in biological fluids and tissues. Antibodies to @entity11015 were raised in @entity95 after administration of an @entity11015 -bovine serum albumin conjugate. The enzymatic tracer was obtained by coupling @entity11015 via its @entity1472 group to acetylcholinesterase (EC 3.1.1.7). These reagents were used for the development of a competitive immunoassay performed on microtitration plates. The limit of quantification was 100 pg/ml in plasma and 1 ng/g in tissues. The plasma assay was performed directly without extraction on a minimal volume of 0.1 ml. The intra- and interassay coefficients of variation were in the range of 5 to 17%, and the recoveries were 92 to 111% for @entity11015 added to @entity1 plasma. The assay was applied to a pharmacokinetic study with @entity19 given @entity11015 intraperitoneally at the dose of 1 mg/kg. The drug distribution in selected compartments (plasma, liver, spleen, lung, and brain) was monitored until 72 h after administration. In conclusion, our assay is at least 100-fold more sensitive than previously described bioassays or chromatographic determinations of @entity11015 and may be useful in studying the tissue pharmacokinetics of new @entity11015 formulations and in drug monitoring in clinical situations.
