Inhibition of @entity788 cell migration by plasma XXXX isolated from healthy volunteers receiving an @entity4211 -rich berry juice.

@entity1 @entity788 @entity1476 @entity5 @entity26 @entity2830 @entity4211 @entity3 @entity1495
PURPOSE: @entity788 is an aggressive @entity5 type, of which the most important characteristics are migration and @entity3 . @entity4211 ( @entity2830 ) are discussed to be protective phytochemicals; however, up to now only scarce data are available regarding their effects on @entity5 prevention. In this study, we aimed to determine whether @entity2830 and their metabolites from plasma (PAM), isolated from blood of healthy volunteers after ingestion of an @entity2830 -rich juice, are effective in modulating @entity5 cell migration in vitro. METHODS: PAM were isolated from blood of healthy volunteers (n  =  10) after consumption of an @entity2830 -rich berry juice. Before ingestion (PAM0min) and after 60  min (PAM60min), blood was taken and PAM were isolated from plasma by solid-phase extraction. Migration of @entity788 cells PANC-1 and AsPC-1 was assayed in a Boyden chamber. The influence of PAM on cellular reactive @entity26 species (ROS) or mitochondria-specific ROS was measured fluorimetrically. mRNA expression levels of matrix metalloproteinases ( @entity1476 and @entity1495 ) and NF-kB mRNA were determined by real-time PCR. RESULTS: After application of PAM60min to PANC-1, we observed a reduced cell migration, which was associated with reduced levels of endogenously generated ROS concomitant with reduced NF-kB as well as @entity1476 and @entity1495 mRNA expression levels. In @entity1 cells, however, migration was not affected by PAM60min. CONCLUSION: It can be assumed that physiologically relevant @entity2830 and their metabolites were able to inhibit @entity788 cell migration in dependency of the phenotype of cells and may thus deserve further attention as potential bioactive phytochemicals in @entity5 prevention.
