Actin Disorganization Plays a Vital Role in Impaired Embryonic Development of In Vitro-Produced XXXX Preimplantation Embryos.

@entity249 @entity18334 @entity19 @entity52905 @entity2493 @entity2389 @entity73069 @entity1379
UNASSIGNED: Assisted reproductive technology (ART) is being increasingly applied to overcome @entity249 . However, the in vitro production process, the main procedure of ART, can lead to aberrant embryonic development and health-related problems in offspring. Understanding the mechanisms underlying the ART-induced side effects is important to improve the ART process. In this study, we carried out comparative transcriptome profiling between in vivo- (IVO) and in vitro- produced (IVP) @entity19 blastocysts. Our results suggested that aberrant actin organization might be a major factor contributing to the impaired development of IVP embryos. To test this, we examined the effect of actin disorganization on the development of IVP preimplantation embryos. Specific disruption of actin organization by @entity2389 (CB) indicated that well-organized actin is essential for in vitro embryonic development. Supplementing the culture medium with 10-9 M @entity2493 , a cytoskeletal modulator in adult somatic cells, significantly reversed the disrupted expression patterns of genes related to actin organization, including @entity52905 , @entity1379 , @entity73069 , @entity18334 , and Palld. Immunofluorescence analysis showed that @entity2493 treatment of IVP embryos significantly improved the distribution and organization of actin filaments (F-actin) from the 8-cell stage onwards. More importantly, we found that @entity2493 alleviated the CB-mediated aberrant F-actin distribution and organization and rescued CB-induced impaired embryonic development. This is the first study to indicate that actin disorganization is implicated in impaired development of IVP embryos during the preimplantation stage. We also demonstrated that improving actin organization is a promising strategy to optimize existing IVP systems.
