XXXX bone marrow-derived dendritic cells generated with @entity7421 / @entity12718 or FLT3L exhibit distinct phenotypical and functional characteristics.

@entity16620 @entity12718 @entity11264 @entity57526 @entity21463 @entity568 @entity567 @entity35 @entity24085 @entity7421 @entity23655 @entity19465
UNASSIGNED: Dendritic cells are professional APCs that play a central role in the initiation of immune responses. The limited ex vivo availability of dendritic cells inspires the widespread use of bone marrow-derived dendritic cells as an alternative in research. However, the functional characteristics of bone marrow-derived dendritic cells are incompletely understood. Therefore, we compared functional and phenotypic characteristics of @entity35 bone marrow-derived dendritic cells generated with @entity7421 / @entity12718 or @entity57526 ligand bone marrow-derived dendritic cells. A comparison of surface markers revealed that @entity57526 ligand-bone marrow-derived dendritic cells expressed signal regulatory protein a, CD103, and @entity11264 and baseline levels of MHC class II, @entity23655 , and @entity24085 , which were highly up-regulated upon stimulation. Conversely, @entity7421 / @entity12718 -bone marrow-derived dendritic cells constitutively expressed signal regulatory protein a, CD11c, and @entity19465 but only mildly up-regulated MHC class II, @entity23655 , or @entity24085 following stimulation. Expression of dendritic cell-associated core transcripts was restricted to @entity57526 ligand-bone marrow-derived dendritic cells . @entity7421 / @entity12718 -bone marrow-derived dendritic cells were superior at phagocytosis but were outperformed by @entity57526 ligand-bone marrow-derived dendritic cells at antigen presentation and T cell stimulation in vitro. Stimulated @entity7421 / @entity12718 -bone marrow-derived dendritic cells secreted more TNF, @entity16620 , @entity21463 , and NO, whereas @entity57526 ligand-bone marrow-derived dendritic cells secreted more @entity568 and @entity567 . Finally, whereas @entity7421 / @entity12718 -bone marrow-derived dendritic cell culture supernatants added to resting T cell cultures promoted forkhead box p3(+) regulatory T cell populations, @entity57526 ligand-bone marrow-derived dendritic cell culture supernatants drove Th17 differentiation. We conclude that @entity35 @entity7421 / @entity12718 -bone marrow-derived dendritic cells and @entity57526 ligand-bone marrow-derived dendritic cells are functionally distinct. Our data support the current rationale that @entity57526 ligand-bone marrow-derived dendritic cells mostly resemble classic dendritic cells but comprise additional minor subpopulations, whereas @entity7421 / @entity12718 -bone marrow-derived dendritic cells resemble monocyte-derived inflammatory dendritic cells (iNOS-positive monocyte-derived cells).
