@entity27773 Plays an Important Role in XXXX -Induced Angiogenesis of Limbs.

@entity5559 @entity63 @entity19 @entity2478 @entity27773 @entity8930 @entity2101 @entity164 @entity1965 @entity1611 @entity731 @entity6580 @entity1995
@entity27773 , lectin-like oxidized low-density lipoprotein (LDL) receptor-1, is a single transmembrane receptor mainly expressed on endothelial cells. @entity27773 mediates the uptake of oxidized LDL, an early step in @entity731 ; however, little is known about whether @entity27773 is involved in angiogenesis during tissue @entity63 . Therefore, we examined the role of @entity27773 in @entity63 -induced angiogenesis in the hindlimbs of @entity27773 knockout (KO) @entity19 . Angiogenesis was evaluated in a surgically induced hindlimb @entity63 model using laser Doppler blood flowmetry (LDBF) and histological @entity2478 ( @entity2478 ) and @entity2101 ( @entity2101 ). After right hindlimb @entity63 , the @entity63 /nonischemic hindlimb blood flow ratio was persistently lower in @entity5559 -1 KO @entity19 than in @entity164 ( @entity164 ) @entity19 . @entity2478 and @entity2101 were significantly smaller in @entity5559 -1 KO @entity19 than in @entity164 @entity19 on postoperative day 14. Immunohistochemical analysis revealed that the number of macrophages infiltrating @entity63 tissues was significantly smaller in @entity5559 -1 KO @entity19 than in @entity164 @entity19 . The number of infiltrated macrophages expressing @entity1611 was also significantly smaller in @entity5559 -1 KO @entity19 than in @entity164 @entity19 . Western blot analysis and ROS production assay revealed that @entity5559 - KO @entity19 show significant decrease in @entity8930 expression, ROS production and @entity1965 expression, the phosphorylation of @entity1995 and NF-kB p65 subunit as well as expression of redox-sensitive @entity6580 ( @entity6580 ) and @entity27773 itself in @entity63 muscles, which is supposed to be required for macrophage infiltration expressing angiogenic factor @entity1611 . Reduction of @entity1611 expression successively suppressed the phosphorylation of Akt and eNOS, which accelerated angiogenesis, in the @entity63 leg of @entity5559 -1 KO @entity19 . Our findings indicate that @entity27773 plays an important role in @entity63 -induced angiogenesis by 1) @entity8930 -ROS-NF-kB activation, 2) upregulated expression of adhesion molecules: @entity6580 and @entity27773 ) promoting macrophage infiltration, which expresses angiogenic factor @entity1611 .
