Early Cellular Changes in the Ascending Aorta and Myocardium in a @entity1707 Model of XXXX .

@entity1707 @entity82 @entity4361 @entity2914 @entity9406 @entity72820 @entity209 @entity413 @entity165
BACKGROUND: @entity209 is associated with pathological remodeling of the heart and adjacent vessels. The early biochemical and cellular changes underlying the vascular damage are not fully understood. In this study, we sought to establish the nature, extent, and initial timeline of cytochemical derangements underlying reduced ventriculo-arterial compliance in a @entity1707 model of @entity209 . METHODS: Yorkshire @entity1707 (n = 8 per group) were fed a @entity4361 ( @entity4361 ) or a high- @entity165 ( @entity2914 ) for 12 weeks. Myocardial function and blood flow was assessed before harvesting the heart. Immuno-blotting and immuno-histochemical staining were used to assess the cellular changes in the myocardium, ascending aorta and left anterior descending artery (LAD). RESULTS: There was significant increase in body mass index, blood @entity413 and mean arterial pressures (p = 0.002, p = 0.001 and p = 0.024 respectively) in @entity2914 group. At the cellular level there was significant increase in anti-apoptotic factors p-Akt (p = 0.007 and p = 0.002) and Bcl-xL (p = 0.05 and p = 0.01) in the @entity2914 aorta and myocardium, respectively. Pro-fibrotic markers TGF-b (p = 0.01), pSmad1/5 (p = 0.03) and @entity9406 (p = 0.005) were significantly increased in the @entity2914 aorta. The levels of pro-apoptotic p38MAPK, @entity72820 and cleaved Caspase3 were significantly increased in aorta of @entity2914 (p = 0.03, p = 0.04 and p = 0.007 respectively). Similar changes in coronary arteries were not observed in either group. Functionally, the high @entity165 diet resulted in significant increase in ventricular end systolic pressure and-dp/dt (p = 0.05 and p = 0.007 respectively) in the @entity2914 group. CONCLUSION: Preclinical @entity209 initiates pro-apoptosis and pro- @entity82 pathways in the heart and ascending aorta, while sparing coronary arteries at this early stage of dietary modification.
