A new method to assess drug sensitivity on XXXX acute slices preparation.

@entity3163 @entity0 @entity39 @entity5 @entity26 @entity1311 @entity3983 @entity6619
A method for assessing @entity5 drug sensitivity is described that is based on preparation of tissue slices and use of @entity6619 chips equipped with electrochemical sensors (multisensor array). The @entity5 slices (200-300 microM thick) are prepared after surgery and incubated in a medium for recovery after slicing. The advantage, compared to other preparations, is that the original three-dimensional structure is retained. Multisensor arrays measure: (a) pericellular acidification (anaerobic metabolism) and (b) @entity26 consumption (respiration). The innovative aspect is that such measurements can be made online, as opposed to using a large battery of endpoint tests on cell vitality and proliferation. Electron microscopy of slices serves to determine cell density and structure and induction of apoptosis/ @entity39 . Slices of more than 200 @entity0 were used. Metabolic activity was inhibited by @entity3163 , which reduces glycolysis, and @entity3983 , which inhibits respiration. These changes are thus reflected in the curves of acidification and @entity26 consumption. In other experiments the cytostatic @entity1311 , an anticytoskeletal agent, was used showing dose and time-dependent effects on acidification and @entity26 consumption. In conclusion, the method presented here, is able to provide information on drug sensitivity of a @entity5 , which aids in designing individualized therapy and is used for drug screening.
