MicroRNA expression in XXXX : the usefulness of @entity406 -fixed, paraffin-embedded material for miRNA microarray profiling.

@entity1 @entity66 @entity5 @entity75 @entity8205 @entity406 @entity320
MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate cellular differentiation, proliferation, and apoptosis. MiRNAs are expressed in a developmentally regulated and tissue-specific manner. Aberrant expression may contribute to pathological processes such as @entity5 , and miRNA may therefore serve as biomarkers that may be useful in a clinical environment for diagnosis of @entity66 . Most miRNA profiling studies have used fresh tissue samples. However, in some types of @entity5 , including malignant @entity320 , fresh material is difficult to obtain from @entity5 , and most surgical specimens are @entity406 fixed and paraffin embedded (FFPE). To explore whether FFPE material would be suitable for miRNA profiling in @entity75 , we compared miRNA expression patterns in FFPE versus fresh frozen samples, obtained from 15 @entity1 @entity8205 . Out of microarray data, we identified 84 miRNAs that were expressed in both types of samples and represented an miRNA profile of @entity8205 . Our results showed a high correlation in miRNA expression (Spearman r-value of 0.80) between paired FFPE and fresh frozen material. The data were further validated by quantitative RT-PCR. In conclusion, FFPE specimens of @entity75 are suitable as a source for miRNA microarray profiling.
