MicroRNAs dysregulation in XXXX malignant pleural mesothelioma.

@entity1 @entity21175 @entity10014 @entity12425 @entity5 @entity2615 @entity15453 @entity12333 @entity36221
BACKGROUND: Malignant @entity2615 (MPM) is a rare but aggressive @entity5 that develops by mesothelial cell transformation. At present, there are no effective therapies for MPM. Great efforts have been made in finding specific markers/mechanisms for MPM onset, including studies into microRNAs (miRNAs). Recent studies have shown the differential expression of mature miRNAs in several @entity1 @entity5 , suggesting their potential role as oncogenes or @entity5 suppressor genes. METHODS: In this study, we investigated miRNAs profile in five @entity1 normal pleural mesothelial short-term cell cultures (HMCs) and five MPMs, with microarray approach. These results were confirmed by real-time quantitative reverse-transcriptase polymerase chain reaction and Western blotting. RESULTS: A comparative analysis of miRNA expression in MPM and HMCs was carried out. Microarray profiling showed different miRNA expression between MPM and HMCs. Specifically, members of the oncomiRNA @entity10014 cluster and its paralogs, namely @entity12425 , 18a, 19b, 20a, 20b, 25, 92, 106a, 106b, were markedly upregulated. Besides, in our investigation, additional miRNAs, such as @entity12333 , @entity21175 , @entity15453 , and @entity36221 were found to be dysregulated in MPM. CONCLUSIONS: These data are in agreement with results that have previously been reported on dysregulated miRNAs for other solid @entity1 @entity5 . Moreover, in our investigation, additional miRNAs were found to be dysregulated in MPM. Interestingly, gene products that regulate the cell cycle are targets and predicted targets for these miRNAs. Our data suggest that specific miRNAs could be key players in MPM development/progression. In addition, some of these miRNAs may represent MPM markers and potential targets for new therapeutic approaches.
