Involvement of Protein XXXX -dependent Kinase, Phospholipase A2 and Phospholipase C in Sperm Acrosome Reaction of Chinchilla lanigera.

@entity268 @entity137 @entity1420 @entity19 @entity1609 @entity1426 @entity1359 @entity4385 @entity23834 @entity5154
UNASSIGNED: The mechanisms involved in fertilization are the centre of attention in order to determine the conditions required to reproduce in  vitro the events that take place in  vivo, with special interest in endangered species. Previous data from @entity19 sperm, where @entity137 ( @entity137 ) occurs more often in the interstitium of the cumulus oophorus, contribute to strengthen the use of @entity1420 as a physiological inducer of this process. We studied the participation of protein kinase A (PKA), phospholipases A2 and C ( @entity23834 , @entity1609 ) in the @entity137 induced by @entity1420 from Chinchilla epididymal spermatozoa. The addition of db- @entity268 to the incubation medium caused an increase of 58% in the @entity137 , while the use of H89 (30   m), a PKA inhibitor, reflected a decrease of 40% in the percentage of reacted gametes. The assays conducted with @entity1426 showed a maximum increase of 23% in the @entity137 . When gametes were pre-incubated with @entity23834 inhibitors, a dose-dependent inhibitory effect was observed. The addition of @entity1359 (10   m) revealed higher percentages of @entity137 induction (60%). When @entity1609 was inhibited with @entity5154 and @entity4385 , a dose-dependent decrease in @entity137 percentages was observed. Combined inhibition of PKA, @entity23834 and @entity1609 , @entity137 values similar to control were obtained. This work shows evidence, for the first time in Chinchilla, that @entity1420 activates the AC/ @entity268 /PKA system as well as sperm phospholipases and that these signalling pathways participate jointly and cooperatively in @entity137 . These results contribute to the understanding of the complex regulation that is triggered in sperm after the effect of @entity1420 .
