Involvement of MAPK and @entity10412 signaling pathway in sterigmatocystin-induced G2 phase arrest in XXXX gastric epithelium cells.

@entity1 @entity10610 @entity8189 @entity3963 @entity584 @entity10609 @entity3470 @entity10412 @entity4675 @entity356
SCOPE: Sterigmatocystin (ST), a mycotoxin commonly found in foodstuff and feedstuff, has been shown to be a carcinogenic mycotoxin in animal models. Many studies showed that the high level of ST contamination in grains might be related to the high incidence of @entity356 in rural areas of China. However, up to now, the potential effects of ST on @entity1 gastric epithelium cells remain largely unknown. In this study, we explored the effects of ST on cell-cycle distribution and the regulatory mechanism in immortalized @entity1 gastric epithelium cells (GES-1). METHODS AND RESULTS: The effects of ST on the cell cycle distribution of GES-1 cells were determined with flow cytometric (FCM) analysis, Giemsa staining and immunofluorescence staining, while that on the expression of related gene- @entity10609 , @entity10610 , @entity8189 and the complex of @entity8189 - @entity10610 were studied with Western blot, reverse transcription polymerase chain reaction (RT-PCR) and immunoprecipitation assay respectively. We found that ST induced GES-1 cells arrested at G2 phase by regulating the expression of @entity10609 , @entity10610 , @entity8189 and the formation of @entity8189 - @entity10610 complex. Further study suggested @entity4675 , @entity3963 and @entity10412 / @entity584 / @entity3470 pathways to be involved in the process of G2 arrest induced by ST. The specific inhibitors of @entity4675 and @entity3963 reversed the role of ST, whereas that of @entity10412 / @entity584 / @entity3470 reinforced the effect of ST on cell-cycle distribution. CONCLUSION: This study demonstrates that @entity4675 , @entity3963 and @entity10412 / @entity584 / @entity3470 pathways participated in the G2 arrest induced by ST through the deregulation of @entity8189 , @entity10610 and @entity10609 . It may play some roles in the @entity356 in ST exposure populations.
