Mitogens are increased in the systemic circulation during bone XXXX .

@entity1 @entity174 @entity442 @entity2533 @entity446
The influence of mechanical tissue strain caused by flexible @entity174 fixation on the systemic occurrence of systemic mitogens during @entity446 was investigated. For this purpose the mitogenic capacity and growth factor concentration of sera from @entity1 undergoing @entity174 treatment were determined. Sera from 9 @entity1 whose @entity174 had been stabilized by external fixation were collected before and during @entity174 treatment. The sera were added to cell culture media of the osteoblastic cell line SaOS-2. After 5-6 days cell proliferation was measured. @entity2533 ( @entity2533 ) and @entity442 ( @entity442 ) concentrations were analyzed in serum samples from different healing stages. STATISTICS: paired Wilcoxon-test. Sera from @entity174 @entity1 decreased SaOS-2 proliferation in the first week after surgery (p<0.05) compared to sera obtained prior to surgery. In the fourth or fifth week proliferation increased significantly (p<0.03). The increased proliferation of the SaOS-2 cells was associated with elevated levels of TGF-beta and @entity442 (p<0.05). The higher mitogenic activity of sera suggests an increased level of circulating mitogens. In a previous study this increase had also been observed in @entity1 during distraction osteogenesis treatment but not in @entity1 with primary bone healing by a stable fixated plate. It is therefore assumed that their release from the @entity174 site is a consequence of mechanical stimulation by interfragmentary movement of @entity174 ends.
