Intravenous Infusion of Monocytes Isolated from 2-Week-Old @entity19 Enhances Clearance of Beta-Amyloid Plaques in an Alzheimer XXXX Model.

@entity32 @entity19 @entity73 @entity10062 @entity9271 @entity2823 @entity179 @entity195 @entity3804
UNASSIGNED: @entity195 ( @entity195 ) is characterized by the deposition of b-amyloid (Ab) senile plaques and tau-associated neurofibrillary tangles. Other disease features include neuroinflammation and cholinergic @entity179 , indicating their possible importance in disease propagation. Recent studies have shown that monocytic cells can migrate into the @entity195 brain toward Ab plaques and reduce plaque burden. The purpose of this study was to evaluate whether the administration of intravenous infusions of 'young' @entity3804 -positive (+) monocytes into an @entity195 @entity19 model can enhance Ab plaque clearance and attenuate @entity73 . Peripheral monocytes were isolated from two-week-old wildtype @entity19 using the Pluriselect @entity3804 + isolation method and characterized by @entity10062 analysis for surface marker expression and effective phagocytosis of 1  m fluorescent microspheres, @entity2823 -Dextran or @entity2823 -Ab1-42. The isolated monocytes were infused via the tail vein into a transgenic @entity195 @entity19 model, which expresses the Swedish, Dutch/Iowa APP mutations (APPSwDI). The infusions began when animals reached 5 months of age, when little plaque deposition is apparent and were repeated again at 6 and 7 months of age. At 8 months of age, brains were analyzed for Ab+ plaques, inflammatory processes and microglial ( @entity9271 ) activation. Our data show that infusions of two-week-old @entity3804 + monocytes into adult APPSwDI @entity19 results in a transient improvement of memory function, a reduction (30%) in Ab plaque load and significantly in small (<20  m) and large (>40  m) plaques. In addition, we observe a reduction in @entity9271 + cells, as well as no marked elevations in cytokine levels or other indicators of @entity32 . Taken together, our findings indicate that young @entity3804 + monocytes may serve as therapeutic candidates for improved Ab clearance in @entity195 .
