DNA array-based transcriptional analysis of asporogenous, nonsolventogenic XXXX strains SKO1 and M5.

@entity6722 @entity3793 @entity19313 @entity1735 @entity50195 @entity164 @entity505 @entity3881
The large-scale transcriptional program of two @entity19313 strains (SKO1 and M5) relative to that of the parent strain ( @entity164 [ @entity164 ]) was examined by using DNA microarrays. Glass DNA arrays containing a selected set of 1,019 genes (including all 178 pSOL1 genes) covering more than 25% of the whole genome were designed, constructed, and validated for data reliability. Strain SKO1, with an inactivated spo0A gene, displays an asporogenous, filamentous, and largely deficient solventogenic phenotype. SKO1 displays downregulation of all solvent formation genes, sigF, and @entity1735 metabolism genes (similar to genes expressed as part of the stationary-phase response in @entity3881 ) but also several electron transport genes. A major cluster of genes upregulated in SKO1 includes abrB, the genes from the major @entity6722 and motility operons, and glycosylation genes. @entity50195 displays an asporogenous and nonsolventogenic phenotype due to loss of the megaplasmid pSOL1, which contains all genes necessary for solvent formation. Therefore, M5 displays downregulation of all pSOL1 genes expressed in the @entity164 . Notable among other genes expressed more highly in @entity164 than in M5 were sigF, several two-component @entity3793 kinases, spo0A, cheA, cheC, many stress response genes, fts family genes, DNA topoisomerase genes, and central- @entity505 metabolism genes. Genes expressed more highly in M5 include electron transport genes (but different from those downregulated in SKO1) and several motility and @entity6722 genes. Most of these expression patterns were consistent with phenotypic characteristics. Several of these expression patterns are new or different from what is known in @entity3881 and can be used to test a number of functional-genomic hypotheses.
