Measurement of serum unconjugated @entity11111 and XXXX by high-performance liquid chromatography.

@entity172 @entity1 @entity11111 @entity1626 @entity53 @entity10819
Measurement of @entity11111 (E3) and @entity53 ( @entity10819 ) within 22 min by high-performance liquid chromatography (HPLC) was achieved in this study, and the values were compared with those of a radioimmunoassay (RIA). A totally computerized HPLC method was developed for measuring unconjugated E3 (u-E3) and @entity10819 ( @entity10819 ) in the sera of pregnant women. The serum samples were injected directly into the apparatus and transferred to a pretreatment column where the @entity172 were absorbed while hydrophilic components such as proteins and carbohydrates were excluded. The @entity172 were then passed through another separation column containing a new type of polymer gel. The mobile phase consisted of an @entity1626 -water mixture, and separation was achieved by means of a reversed-phase procedure. The eluate was monitored for fluorescence. All procedures were monitored and controlled with a built-in microcomputer. Serum samples from 97 normal pregnant @entity1 at 20-41 wk gestation were simultaneously assayed by HPLC and RIA. The correlations obtained by HPLC and RIA were as follows: u-E3, y = 0.905x - 0.385, with a coefficient of correlation of r = 0.912; for @entity10819 , y = 0.964x + 5.024, with a coefficient of correlation of r = 0.841 (y, RIA value; x, HPLC value). The quick measurement of u-E3 and @entity10819 by HPLC can be a useful method for evaluating fetoplacental function.
