XXXX modulates epithelial-to-mesenchymal transition by targeting @entity18436 and @entity24852 during peritoneal dialysis.

@entity1 @entity82 @entity3670 @entity5962 @entity958 @entity34733 @entity2533 @entity2277 @entity24852 @entity18436 @entity298
UNASSIGNED: @entity298 ( @entity298 ) is the most readily feasible home-dialysis method for renal replacement therapy. However, repeated use of @entity298 can lead to induction of mesothelial/epithelial-mesenchymal transition (MMT/ @entity958 ) and @entity82 , eventually leading to ultrafiltration failure and discontinuation of @entity298 . @entity34733 ( @entity34733 ) is believed to be a potent downstream inhibitor of @entity2533 in renal @entity82 , but the effect of @entity34733 on MMT/ @entity958 relevant to @entity298 is unknown. In this study, as determined by microRNA array analysis and confirmed by northern blot analysis and real-time PCR, we demonstrate that miRNA-129-5p is decreased in mesothelial cells isolated from effluent of @entity1 having @entity298 for more than 6 months extending to several years compared with those who have undergone @entity298 for less than 6 months. The decreased expression of @entity34733 was accompanied with alterations in @entity958 -related genes and the expression of respective proteins in vivo. In addition, in in vitro studies we noted that the expression of @entity5962 and @entity3670 were significantly reduced with increased cell migration in HMrSV5, a @entity1 peritoneal mesothelial cell line (HPMC), treated with @entity2533 , whereas expression of @entity2277 , fibronectin and transcription factors @entity18436 and @entity24852 increased significantly, as assessed by real-time PCR, western blot analysis and immunofluorescence microscopy. Furthermore, alteration in @entity958 -related genes and proteins were reversed by overexpression of @entity34733 . No effect was observed in cells treated with miR-negative control. Meanwhile, inhibition of @entity18436 and @entity24852 with their respective siRNA also could decrease the expression of @entity958 -related genes and protein levels in HPMCs induced with @entity2533 . Finally, we demonstrate that @entity18436 can inhibit the promoter activity of @entity5962 while enhancing the promoter activity of @entity2277 . We also observed that @entity34733 could directly target the 3'UTR of @entity18436 and @entity24852 genes, and repressed their post-transcriptional activities. These data suggest that there is a novel @entity2533 / @entity34733 / @entity18436 or @entity24852 pathway that has a significant role in MMT and @entity82 in the setting of @entity298 .Laboratory Investigation advance online publication, 11 May 2015; doi:10.1038/labinvest.2015.57.
