Comparison of the effect of oestradiol, @entity440 and @entity441 on nerve growth factor-alpha expression in specific neonatal XXXX uterine cell types using laser capture microdissection.

@entity440 @entity26303 @entity19 @entity1870 @entity2659 @entity20384 @entity10788 @entity669 @entity441
Oral dosing of @entity2659 @entity19 on days 2-5 after birth with @entity440 but not @entity441 disrupts the development of the myometrium, resulting in adult uterine @entity1870 . Using laser capture microdissection and RT-PCR we have investigated @entity10788 ( @entity10788 ) and cognate receptor expression in uterine cells of 6-day-old pups that may be important in early developmental changes that give rise to @entity1870 . @entity10788 down-regulation is known to occur during terminal myogenic differentiation. @entity10788 was found exclusively in endometrial luminal epithelium of controls. It was up-regulated 18-fold in the luminal epithelium following dosing with @entity440 but not @entity441 . Western blotting for @entity10788 protein in the whole uterus showed a 25-fold increase after @entity440 treatment. Expression of the low affinity p75 neutrophin receptor ( @entity20384 ) was twofold higher in the myometrium compared with luminal epithelium or stroma. This was not altered following @entity440 treatment. There was no detectable expression of high affinity @entity669 kinase receptor ( @entity26303 ( @entity20384 )). This study shows luminal epithelial cells of the endometrium primarily form @entity10788 . This suggests that @entity10788 normally regulates the differentiation of the mesenchyme into uterine myocytes through paracrine mechanisms and that an early disturbance of this process plays a key role in the subsequent development of @entity1870 .
