Implantation of a Tissue-Engineered Neo-Bile Duct in XXXX .

@entity2835 @entity1707 @entity12526 @entity298 @entity254 @entity5906 @entity648 @entity3929 @entity2876 @entity1011 @entity255
BACKGROUND: Extrahepatic bile duct injuries remain severe complications during cholecystectomies and often require reconstruction by bilioenteric @entity254 (i.e., hepaticojejunostomy), which comes with further long-term complications (e.g., recurring @entity255 , @entity2835 ). In the case of inherent @entity2876 or during liver transplant, artificial or engineered bile ducts could allow novel surgical strategies without the need for hepaticojejunostomy. METHODS: We present data on the implantation of in vitro-generated neo-bile ducts in 5 @entity12526 . The neo-bile ducts were engineered through decellularization of allogeneic blood vessels and recellularization with autologous cholangiocytes. On postoperative days 0, 1, 7, and 14, blood samples were taken and analyzed ( @entity5906 aminotransferase, @entity3929 aminotransferase, @entity648 , alkaline phosphatase, @entity1011 , and leukocytes). Magnetic resonance cholangiopancreatography was performed on postoperative day 14 on 1 @entity1707 . Fourteen days after implantation, the @entity1707 were sacrificed and the bile ducts were explanted. RESULTS: All @entity1707 survived the complete study period without severe complications. None of the @entity1707 showed signs of biliary leakage or @entity298 . The neo-bile ducts were infiltrated by neutrophils, and neoangiogenesis was observed around and into the implanted tissue. CONCLUSION: We present a novel strategy for extrahepatic bile duct replacement by implantation of an autologous neo-bile duct generated ex vivo. Whether the presented technique allows the long-term replacement of native bile ducts must be further evaluated.
