Specific growth inhibition by XXXX of an @entity526 expressing Era-dE, a dominant negative Era mutant.

@entity268 @entity2125 @entity2228 @entity15706 @entity2974 @entity598 @entity12837 @entity563 @entity505 @entity2388 @entity526
@entity526 Era is a GTP binding protein and essential for cell growth. We have previously reported that an Era mutant, designated Era-dE, causes a dominant negative effect on the growth and the loss of the ability to utilize @entity2974 cycle metabolites as @entity505 source when overproduced. To investigate the role of Era, the gene expression in the cells overproducing Era-dE was examined by DNA microarray analysis. The expression of lipA and nadAB, which are involved in @entity12837 synthesis and @entity2125 synthesis, respectively, was found to be reduced in the cells overproducing Era-dE. @entity12837 and @entity2125 are essential cofactors for the activities of @entity2388 dehydrogenase complex, @entity15706 dehydrogenase complex and @entity2228 cleavage enzyme complex. The expression of numerous genes involved in dissimilatory @entity505 metabolism and @entity505 source transport was increased. This set of genes partially overlaps with the set of genes controlled by @entity268 -CAP in @entity526 . Moreover, the growth defect of Era-dE overproduction was specifically enhanced by @entity598 but not by @entity2974 cycle metabolites both in rich and synthetic media. Intracellular @entity563 pool in Era-dE overproducing cells was found to be increased significantly compared to that of the cells overproducing wild-type Era. It was further found that even the wild-type @entity526 cells not overproducing Era-dE became sensitive to @entity598 in the presence of @entity563 in a medium. We propose that when Era-dE is overproduced, @entity505 fluxes to the @entity2974 cycle and to C1 units become impaired, resulting in a higher cellular @entity563 concentration. We demonstrated that such cells with a high @entity563 concentration became sensitive to @entity598 , however the reason for this @entity598 sensitivity is not known at the present.
