XXXX surface topography affects collagen biosynthesis of adherent cells.

@entity1 @entity535 @entity16911 @entity13239 @entity6286 @entity12553 @entity622 @entity10783 @entity702
Collagen-dependent microstructure and physicochemical properties of newly formed bone around implant surfaces represent key determinants of implant biomechanics. This study investigated the effects of implant surface topography on collagen biosynthesis of adherent @entity1 mesenchymal stem cells (hMSCs). hMSCs were grown for 0 to 42 days on @entity702 disks (20.0   1.0 mm) with smooth or rough surfaces. Cell attachment and spreading were evaluated by incubating cells with Texas-Red-conjugated @entity6286 antibody. Quantitative real-time PCR was used to measure the mRNA levels of @entity13239 and collagen modifying genes including @entity10783 hydroxylases (PHs), @entity12553 oxidases (LOXs) and @entity12553 hydroxylases (LHs). Osteogenesis was assessed at the level of osteoblast specific gene expression and @entity622 staining for mineralization. Cell layer-associated matrix and collagen content were determined by @entity535 analysis. At 4h, 100% cells were flattened on both surfaces, however the cells on smooth surface had a fibroblast-like shape, while cells on rough surface lacked any defined long axis. PH, LH, and most @entity16911 mRNA levels were greater in hMSCs grown on rough surfaces for 3 days. The mineralized area was greater for rough surface at 28 and 42 days. The collagen content (percent total protein) was also greater at rough surface compared to smooth surface at 28 (36% versus 26%) and 42 days (46% versus 29%), respectively (p<.05). In a cell culture model, rough surface topography positively modulates collagen biosynthesis and accumulation and the expression of genes associated with collagen cross-linking in adherent hMSC. The altered biosynthesis of the collagen-rich ECM adjacent to endosseous implants may influence the biomechanical properties of osseointegrated endosseous implants.
