Efficacy of a XXXX gD DNA vaccine for induction of humoral and cellular immune responses in Japanese macaques.

@entity22421 @entity1 @entity3307 @entity1651
It is desirable to prevent dissemination of @entity22421 ) in macaque colonies because transmission of BV to @entity1 causes deadly @entity1651 . Vaccination of monkeys is one method that could confine spread of BV within macaque colonies. Availability of a BV DNA vaccine for use in macaques would eliminate the risk of working with infectious BV. Toward this end, we constructed a plasmid expressing the BV glycoprotein D (gD). Immunogenicity of this construct as a DNA vaccine was assessed in adult Japanese macaques by four intracutaneous injections at a dose of 500 microg per head. Results of enzyme-linked immunosorbent assay (ELISA) using a recombinant @entity3307 ) gD, a homologue of BV gD, showed that significant levels of antibody was induced in all vaccinated animals following each booster injection. Western blot of sera from vaccinated macaques confirmed the specific recognition of authentic BV gD. Immune sera were also demonstrated to contain neutralizing activity against infectious BV. Weak lymphoproliferative responses were also observed in vaccinated macaques using recombinant @entity3307 gD as a stimulating antigen and flow cytometry analysis of one individual revealed the presence of @entity3307 gD-responsive effector T cells. Thus, the BV gD DNA vaccine was demonstrated to induce both humoral and cellular immune responses in macaques which recognized BV gD.
