Predicting of Effective Dose as Biomarker for XXXX Using Partial Least Square-Fourier Transform Infrared Spectroscopy (PLS_FTIR).

@entity137 @entity900 @entity2774 @entity2357 @entity902 @entity6244
@entity137 bioassays are important tools to determine biological effects of chemical agents on species. The questions remained on, what effects have been imposed on each of the different molecular site of cells by chemical exposure and how to find a pattern for chemical @entity137 . To address the questions, HepG2 cell lines were exposed to the different concentrations of @entity900 for 24 hours to result cell mortality in the range of one to one hundred percent. Fourier Transform Infrared spectroscopy (FTIR) has been used in this study to analyze the chemical alterations on HepG2 cell line by @entity900 . @entity2357 ( @entity2357 ) analysis was then applied to the FTIR spectrum results to search for a biomarker peak and present the desire cellular effects of @entity900 . The comparison of cellular FTIR spectra after exposure to different concentrations of @entity900 confirmed the binding of @entity900 to DNA through direct interaction of @entity902 to @entity2774 and @entity6244 bases of DNA. Biochemical Index Spectra (BIS) were defined based on the differences between of normal and @entity900 exposed cells. Information from the BIS was subjected to @entity2357 analysis to trigger any particular relationship between the @entity137 spectral response and @entity900 concentration. This approach was capable of predicting the concentration of @entity900 for any particular effects observed in the cellular FTIR spectrum (R(2) = 0.968    0.037). Our work supports the promises that, FTIR can demonstrate the trace of @entity137 before the cells dies. Finally, @entity2357 of FTIR data directly predicts the effective concentration of chemicals in particular cellular components.
