XXXX chemiluminescence assay as an adjunctive tool for assessment of various stages of inflammation: a study of quiescent inflammatory cells.

@entity137 @entity4563 @entity6638 @entity3440 @entity12487
A simple, fast, precise and biologically relevant @entity137 assay for screening @entity137 of minerals would have distinct advantages due to its cost benefits and relative savings in time. Furthermore, a bioassay to differentiate acute and chronic in vivo pulmonary reactions could have potential value as predictors of fibrogenicity and pathogenicity. In this study we examined the potential use of @entity12487 as a probe to evaluate the correlation between chemiluminescence (CL) generated by alveolar macrophages with the known @entity137 and patho genicity by conventional bioassays. In this study, we used small doses of dust (20 microg) to minimize cellular overload and to maintain homeostasis. Crystalline @entity3440 a highly fibrogenic dust was used as positive control and results are compared with those for @entity6638 , @entity4563 and talc. Among the three minerals compared with @entity3440 , @entity6638 was more reactive (27%) in CL assay and declined sharply compared to other minerals. This sudden decline in @entity6638 CL is caused by @entity137 leading to cell death. CL-induced by talc was comparable to @entity3440 and declines slowly. @entity4563 on the other hand produced relatively a weaker (25%) CL compared to @entity3440 . Our data using relatively low doses of dust suggest that the CL assay may have a better predictive value in @entity137 evaluations compared to conventional @entity137 assays.
