A New Technique for Collection of Cerebrospinal Fluid in XXXX Pups.

@entity1545 @entity21078 @entity75 @entity519 @entity35
BACKGROUND: Neuroprotective strategies to prevent or decrease @entity1545 in @entity519 ischemic newborns are one of the main research lines in neonatology. Animal models have been used to assess the efficiency of new therapeutic strategies. @entity75 biomarkers in cerebrospinal fluid (CSF) are frequently used to evaluate the outcome at the bedside. Despite the importance of this approach in clinical practice, there are many difficulties in using it in small animals. The aim of this paper was to describe a new technique for collecting CSF in @entity35 pups. Furthermore the reference values of @entity21078 protein levels, commonly used in common clinical practice, were analyzed in animals between 7 to 12 days. METHODS: 42 @entity35 @entity35 pups aged 7 to 12 days were used. CSF was obtained by direct puncture of the cisterna magna with a 24-gauge needle. @entity21078 protein levels were determined with enzyme-linked immunosorbent assay (ELISA). RESULTS: CSF was successfully obtained in 96% of the cases, with an average amount of 21.28  l (5-40  l). Normal values for @entity21078 were described. HI animals presented higher @entity21078 values than controls. CONCLUSIONS: A simple, reproducible technique for CSF collection in @entity35 pups has been described. This new method will allow study of @entity1545 biomarkers in newborn @entity519 ischemic animal models.
