Potential Association of Urinary XXXX with Dietary @entity3323 Intake of Smokers and Nonsmokers.

@entity1 @entity37072 @entity37073 @entity3323 @entity1011 @entity71952
@entity3323 (AA), a rodent carcinogen, is widely used in industry and present in cigarette smoke as well as in foods processed at high temperatures. The metabolic activation of AA to @entity37072 (GA) could be critical for AA carcinogenicity since GA causes DNA adduct formation in vivo. @entity37073 (N7-GAG), the most abundant DNA adduct of AA, is subjected to spontaneous and enzymatic depurination and excreted through urine. Urinary N7-GAG analysis can confirm AA genotoxicity and identify active species of AA metabolites in @entity1 , thereby serving as a risk-associated biomarker for molecular epidemiology studies. This study aimed to develop an isotope-dilution solid-phase extraction liquid chromatography tandem mass spectrometry method to comparatively analyze urinary N7-GAG levels in nonsmokers and smokers. Urinary @entity71952 (AAMA), a metabolite of AA, was also analyzed as a biomarker for current AA exposure. Urinary N7-GAG was quantified by monitoring m/z 239  >  152 for N7-GAG and m/z 242  >  152 for (13)C3-labeled N7-GAG under positive electron spray ionization and multiple reaction mode. The median urinary N7-GAG level was 0.93  g/g @entity1011 in nonsmokers (n = 33) and 1.41  g/g @entity1011 in smokers (n = 30). Multiple linear regression analysis of data revealed that N7-GAG levels were only significantly associated with AAMA levels. These results demonstrate that urinary N7-GAG of nonsmokers and smokers is significantly associated with a very low level of dietary AA intake, assessed by analyzing urinary AAMA.
