Critical enhancer region to which @entity5874 / @entity32299 and @entity3419 bind in the XXXX @entity4188 gene.

@entity1 @entity43 @entity0 @entity2555 @entity4188 @entity5874 @entity32299 @entity157 @entity3419 @entity1300 @entity694
@entity4188 is known to be induced by @entity2555 including @entity1300 ( @entity1300 ). The constitutive and @entity1300 -inducible transcriptional expression of @entity1 @entity4188 is known to be cell-specific. In order to identify the cis-elements that cell-specifically regulate the constitutive and @entity1300 -inducible transcription of @entity4188 , we constructed luciferase reporter plasmids containing a series of deletions of the XRE core sequence in the 5'-flanking region of the @entity1 @entity4188 gene. Luciferase assays were performed with MCF-7 ( @entity0 ), HepG2 ( @entity157 ), LS-180 ( @entity694 ), and OMC-3 ( @entity43 ) cells. Although there were large differences in the relative luciferase activity and inducibility between these four cell lines, the contribution of each reporter construct was similar. Constitutive expression increased with the regulatory elements that are present at -910 to -852 and -1652 to -1243. Potential enhancer elements for @entity1300 -induction were located from -1022 to -852 including three XREs, XRE3 at -853, XRE4 at -940, and XRE5 at -989. Gel shift analyses revealed binding of the @entity5874 / @entity32299 heterodimer to XRE2 at -834, XRE3 at -853, XRE6 at -1024, and XRE7 at -1490. In addition, the binding of a nuclear transcriptional factor, @entity3419 , near XRE2 and XRE8 was observed. It was suggested that mutual interaction of XRE2 and XRE3 is important for transcriptional regulation, and that the @entity3419 binding to the @entity3419 -like motif (-824) enhances both the constitutive and inducible transcriptional activities of the @entity1 @entity4188 gene.
