@entity1420 promotes cell migration, invasion and XXXX activation in @entity1 @entity816 cells.

@entity1 @entity12386 @entity0 @entity1420 @entity1313 @entity5 @entity816 @entity3927 @entity463
UNASSIGNED: Astrocytomas are the most common and aggressive @entity1313 in @entity1 . Invasiveness of these @entity5 has been attributed in part to deregulation of cell motility-dependent cytoskeletal dynamics that involves actin-binding proteins such as @entity12386 . @entity1420 (P4) has been found to induce migration and invasion of cells derived from @entity0 and endothelium. However, the role of P4 in migration and invasion of @entity816 cells as well as its effects on @entity816 cytoskeleton remodeling is not known. In this work we evaluated these aspects in D54 and U251 cells derived from @entity1 @entity816 from the highest degree of @entity5 (grade IV, @entity463 ). Our results showed that in scratch-wound assays P4 increased the number of D54 and U251 cells migrating from 3 to 48h. Both @entity3927 , a P4 receptor (PR) antagonist, and an oligonucleotide antisense against PR significantly blocked P4 effects. Transwell assays showed that P4 significantly increased the number of invasive cells at 24h. As in the case of migration, this effect was blocked by @entity3927 . Finally, by Western blotting, an increase in the @entity12386 /p- @entity12386 ratio at 15 and 30min and a decrease at 30 and 60min in U251 and D54 cells, respectively, was observed after P4, P4+ @entity3927 and @entity3927 treatments. These data suggest that P4 increases @entity1 @entity816 cells migration and invasion through its intracellular receptor, and that @entity12386 activation by P4 is independent of PR action.
