Quantification of HLA class I molecules on XXXX using Edman degradation.

@entity1 @entity8 @entity1531 @entity5 @entity1358 @entity3
BACKGROUND: Unimpaired @entity1531 presentation is a prerequisite for the recognition of @entity5 cells by cytotoxic T lymphocytes and thus essential for the success of anticancer immunotherapeutic concepts. Several approaches have been taken in the immunotherapy of @entity1358 ( @entity1358 ), however of limited success. HLA loss or down-regulation have often been reported and might interfere with immunotherapeutic approaches aimed at the recognition of HLA-presented peptides. METHODS: We employed a quantitative method of molecular analysis for the comparison of HLA amounts on @entity5 , normal kidney and @entity3 of @entity1358 , using Edman degradation. We analyzed a series of 47 @entity1358 samples including corresponding @entity8 , local lymph node @entity3 and distant @entity3 . RESULTS: Results of quantitative Edman degradation revealed significantly higher HLA yields on @entity5 and @entity3 compared to normal kidney tissue. This effect was shown not to result from infiltrating immune cells, since @entity5 -infiltrating lymphocytes had no influence on the overall HLA recovery from @entity5 tissue. Unexpectedly, we found a higher amount of HLA class I molecules on distant @entity3 compared to local lymph node @entity3 . CONCLUSION: Edman degradation allows the direct quantitative comparison of HLA class I protein expression by @entity5 or normal tissue and @entity3 of @entity1358 @entity1 . Our results raise hopes for improving the success and effectiveness of future immunotherapeutic concepts for metastatic @entity1358 .
