The effects of Th17 cytokines on the inflammatory mediator production and barrier function of XXXX cells.

@entity1 @entity650 @entity11178 @entity32 @entity16365 @entity13236 @entity29716 @entity11177 @entity1596 @entity2822 @entity63719 @entity2842 @entity4155 @entity13771 @entity2823 @entity4654 @entity13235 @entity989 @entity687 @entity792
Th17 cells have emerged as a key factor in the pathogenesis of @entity1596 as well as other @entity792 . They secrete a number of cytokines including @entity4155 , @entity4654 and @entity13236 and until now the effects of these cytokines on resident cells of the eye were not yet clear. The purpose of this study was to investigate the effects of Interleukin (IL)-17A, @entity4654 and @entity13236 on the production of inflammatory mediators and barrier function of @entity989 cells. We showed that @entity1 cells, a spontaneously arisen cell line of @entity989 ( @entity989 ), constitutively expressed @entity13771 and @entity13235 , but not @entity29716 . @entity4155 significantly enhanced the production of @entity687 , @entity2842 , @entity16365 and @entity650 by these cells. @entity4654 had a similar effect on the production of @entity687 , @entity2842 and @entity650 by @entity1 cells, but did not influence the expression of @entity16365 . Both @entity4155 and @entity4654 significantly decreased the transepithelial electrical resistance (TER) of the ARPE-19 monolayer and increased the diffusion rate of @entity2822 ( @entity2823 )-dextran. They also disrupted the distribution of the junction proteins @entity11178 and @entity11177 at the interface of adjacent cells. @entity13236 did not have a detectable effect on the production of the tested inflammatory mediators by @entity1 cells, TER of the ARPE-19 monolayer, the diffusion rate of @entity2823 -dextran or the distribution of @entity63719 . This study demonstrates that @entity4155 and @entity4654 , but not @entity13236 , significantly promoted @entity1 cells to secrete inflammatory mediators and compromised the ARPE-19 monolayer barrier function in association with a disrupted distribution of @entity63719 . These results suggest that both @entity4155 and @entity4654 may play a role in @entity32 of the eye.
