@entity1836 complements XXXX genes that facilitate the switch from glycolytic to oxidative metabolism.

@entity1 @entity1836 @entity5264 @entity1735 @entity505 @entity375 @entity413
All eukaryotic organisms have mechanisms to adapt to changing metabolic conditions. The @entity1 cell survival gene @entity1836 enables cells to adapt to changes in cellular metabolism. To identify genes whose function can be substituted by @entity1836 in a unicellular eukaryote, a genetic screen was performed using the @entity375 @entity375 . @entity375 grows by anaerobic glycolysis when @entity413 is available, switching to oxidative phosphorylation when @entity1735 in the media becomes limiting (diauxic shift). Given that @entity1836 appears to facilitate the switch from glycolytic to oxidative metabolism in @entity1 cells, a library of @entity375 mutants was tested for the ability to efficiently undergo diauxic shift in the presence and absence of @entity1836 . Several mutants were identified that have a defect in growth when switched from a fermentable to a nonfermentable @entity505 source that is corrected by the expression of @entity1836 . These genes include the mitochondrial chaperonin TCM62, as well as previously uncharacterized genes. One of these uncharacterized genes, SVF1, promotes cell survival in @entity1 cells in response to multiple apoptotic stimuli. The finding that TCM62 and the analogous @entity1 @entity5264 gene also inhibit @entity1 cell death following growth factor withdrawal implicates mitochondrial chaperones as regulators of apoptosis. Further characterization of the genes identified in this screen may enhance our understanding of @entity1836 function in @entity1 cells, and of cell survival pathways in general.
