The Impact of Stannous, XXXX Ions and Its Combination on Enamel Pellicle Proteome and @entity2681 Prevention.

@entity18360 @entity2681 @entity1792 @entity7034 @entity1388 @entity291
OBJECTIVES: To compare the effects of stannous (Sn) and @entity1388 (F) ions and their combination on acquired enamel pellicle (AEP) protein composition (proteome experiment), and protection against @entity2681 (functional experiment). METHODS: In the proteome experiment, @entity291 enamel specimens were incubated in whole saliva supernatant for 24h for AEP formation. They were randomly assigned to 4 groups (n=10), according to the rinse treatment: Sn (800ppm/6.7mM, @entity18360 ), F (225ppm/13mM, @entity7034 ), Sn and F combination (Sn+F) and deionized water (DIW, negative control). The specimens were immersed 3* in the test rinses for 2min, 2h apart. Pellicles were collected, digested, and analyzed for protein content using liquid chromatography electrospray ionization tandem mass spectrometry. In the functional experiment, @entity291 enamel specimens (n=10) were similarly treated for pellicle formation. Then, they were subjected to a five-day @entity2681 cycling model, consisting of 5min erosive challenges (15.6 mM @entity1792 , pH 2.6, 6*/d) and 2min treatment with the rinses containing Sn, F or Sn+F (3*/d). Between the treatments, all specimens were incubated in whole saliva supernatant. Surface loss was determined by profilometry. RESULTS: Our proteome approach on @entity291 enamel identified 72 proteins that were common to all groups. AEP of enamel treated with Sn+F demonstrated higher abundance for most of the identified proteins than the other groups. The functional experiment showed reduction of enamel surface loss for Sn+F (89%), Sn (67%) and F (42%) compared to DIW (all significantly different, p<0.05). CONCLUSION: This study highlighted that anti- @entity2681 rinses (e.g. Sn+F) can modify quantitatively and qualitatively the AEP formed on @entity291 enamel. Moreover, our study demonstrated a combinatory effect that amplified the anti-erosive protection on tooth surface.
