Microarray analysis of differentially expressed genes in XXXX from risk-reducing salpingo-oophorectomies.

@entity43 @entity1 @entity5344 @entity5345 @entity2674 @entity189 @entity6069 @entity2670
UNASSIGNED: Mutations in the @entity5345 and @entity2674 genes confer an increased lifetime risk for @entity5344 . @entity43 risk can be decreased by risk-reducing salpingo-oophorectomy (RRSO). Studies on RRSO material have altered the paradigm of serous @entity43 pathogenesis. The purpose of this study was to identify candidate genes possibly involved in the pathogenesis of serous @entity43 by carrying out a microarray analysis of differentially expressed genes in @entity6069 - mutation positive @entity2670 derived from RRSO surgery. Freshly frozen @entity2670 samples from nine @entity5345 /2 mutation carriers scheduled for RRSO were prospectively collected together with five mutation-negative control @entity1 undergoing salpingo-oophorectomy for benign indications. Microarray analysis of genome-wide gene expression was performed on @entity2670 samples from the @entity6069 and control @entity1 . The validation of microarray data was performed by quantitative real-time polymerase chain reaction (qRT-PCR) in selected cases of RRSO samples and also in high grade @entity189 samples collected from @entity1 with a BRCA phenotype. From 22,733 genes, 454 transcripts were identified that were differentially expressed in @entity5345 /2 mutation carriers when compared with controls, pooling all @entity2670 samples together. Of these, 299 genes were statistically significantly downregulated and 155 genes upregulated. Differentially expressed genes in @entity6069 samples reported here might be involved @entity43 and provide interesting targets for further studies.    2015 Wiley Periodicals, Inc.
