microRNA-218 promotes @entity136 sensitivity in XXXX @entity788 cells by regulating @entity518 expression.

@entity1 @entity518 @entity788 @entity14113 @entity136
OBJECTIVE: The purpose of this study was to examine the effect of @entity136 ( @entity14113 ) on microRNA-218 (miR-218) expression in @entity1 @entity788 cells. METHODS: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to examine the differences in miR-218 expression between the @entity14113 -sensitive BxPC-3 @entity788 cells and @entity14113 -resistant PANC-1 cells. The effect of @entity14113 on the expression of miR-218 in PANC-1 cells was also investigated. PANC-1 cells were transfected either with @entity518 siRNA to knock down the expression of @entity518 or with the recombinant @entity518 expression vector (pcDNA3.1- @entity518 ) to overexpress @entity518 . The effect of ectopic expression of @entity518 on the apoptosis of miR-218-transfected and @entity14113 -treated PANC-1 cells was examined by flow cytometric analysis. RESULTS: The miR-218 expression level was lower in @entity14113 -resistant PANC-1 cells compared to @entity14113 -sensitive BxPC-3 cells (P<0.05). The percentage of apoptotic PANC-1 cells was significantly increased in the miR-218 mimic + @entity14113 group compared to the mimic ctrl + @entity14113 group and the normal control group (P<0.01). The @entity518 expression level was markedly decreased in PANC-1 cells transfected with @entity518 siRNA but was significantly increased in PANC-1 cells transfected with the recombinant @entity518 expression vector, pcDNA3.1- @entity518 (P<0.01). The proportion of apoptotic PANC-1 cells was significantly lower in the miR-218 mimic + @entity14113 + pcDNA3.1- @entity518 group compared to the miR-218 mimic + @entity14113 + @entity518 siRNA group (P<0.01). CONCLUSIONS: The expression level of miR-218 was downregulated in the @entity14113 -resistant cell line. miR-218 promoted the sensitivity of PANC-1 cells to @entity14113 , which was achieved mainly through regulating the expression of @entity518 in PANC-1 cells.
