[Correlation of cell cycle alteration to @entity4635 gene demethylation induced by XXXX in @entity1582 cell lines].

@entity4635 @entity4447 @entity1 @entity9188 @entity5 @entity1582 @entity98
BACKGROUND _ OBJECTIVE: Recent studies suggest strong therapeutic potentials of @entity4447 ( @entity9188 ) for @entity1582 (MM), which may be due to @entity9188 -induced demethylation of @entity5 suppressor genes. This study was to explore the correlation of cell cycle alteration to @entity4635 gene demethylation after @entity9188 induction in MM cell lines in vitro. METHODS: MM cell lines @entity1 and RPMI8226 were used. Cell proliferation and cell cycle of MM cells after the treatment of @entity9188 were assessed by @entity98 assay and flow cytometry, respectively. Methylation status was detected by methylation specific PCR (MSP-PCR), and gene expression of @entity4635 was measured by real-time PCR in MM cells before and after @entity9188 treatment. RESULTS: @entity9188 significantly inhibited the growth of @entity1 and RPMI8226 cells in a dose-dependent manner. The cell cycle of U266 and RPMI8226 were arrested at G0/G1 phase. Compared with the wild type, the percentage of cells was increased at G0-G1 phase, but decreased at S phase after the treatment of @entity9188 for 72 h (P < 0.05). The mRNA expression of @entity4635 gene was significantly increased with hemi-methylation ( @entity9188 , 0.5 micromol/L,72 h) or complete demethylation ( @entity9188 , 1.0 micromol/L or @entity9188 , 2.0 micromol/L,72 h) of the @entity4635 gene in comparison with the wide type (P<0.05). CONCLUSIONS: @entity9188 could induce cell cycle alteration of MM, which might be related to demethylation and reexpression of @entity4635 gene in MM cell lines. The study might provide a new approach to elucidate the mechanism of the antitumor effect of @entity9188 in MM.
