@entity9331 -Related Peptide Vaccine Protects Against @entity15395 -Induced XXXX Formation and Rupture.

@entity6970 @entity449 @entity1755 @entity1750 @entity19 @entity15395 @entity636 @entity9331 @entity777 @entity1961 @entity731 @entity42346
BACKGROUND: T cells and macrophages are implicated in the pathogenesis of @entity449 ( @entity449 ) and @entity731 . We recently demonstrated that a vaccine using an @entity9331 -related peptide @entity42346 reduces @entity731 with favorable modulation of CD8(+) T cells in @entity6970 -deficient (apoE(-/-)) @entity19 . OBJECTIVES: This study hypothesized that a @entity42346 vaccine could reduce @entity449 formation in the @entity15395 ( @entity15395 )-induced @entity449 model. METHODS: Male apoE(-/-) @entity19 were immunized with @entity42346 vaccine and implanted with an @entity15395 -releasing pump for 4  weeks. Flow cytometry assessed T cell activation and phenotype. @entity777 ( @entity777 ) and @entity1750 ( @entity1750 ) expression were assessed using reverse transcription polymerase chain reaction. We used ex  vivo aortic explants to test monocyte adhesion and in  vitro cocultures to evaluate CD8(+) T cell function. RESULTS: The @entity42346 vaccine activated CD8(+) T cells and reduced @entity449 formation and mortality due to @entity1755 , which was attenuated by CD8(+) T cell depletion. Vaccination decreased expression of @entity777 and @entity1750  and reduced macrophage infiltration in the aorta. Cytotoxic T-lymphocyte assay showed that CD8(+) T cells from @entity42346 -immunized @entity19 had higher lytic activity against @entity15395 -stimulated macrophages. The @entity42346 vaccine decreased splenic Th17 cells, and in  vitro coculture of @entity1961 (+) and CD8(+) T cells showed that CD8(+) T cells from @entity42346 -immunized @entity19 inhibited the polarization of @entity1961 (+) T cells into Th17 cells. @entity636 (-/-) @entity19 infused with a higher dose of @entity15395 did not develop @entity1755 . CONCLUSIONS: A @entity42346 vaccine protected against @entity15395 -induced @entity449 formation and mortality by reducing macrophage infiltration in the aorta and decreasing Th17 cell polarization. Our findings provide a potentially novel immunomodulating approach against @entity449 .
