Transforming Growth Factor-b1 Induced Urethral @entity82 in a XXXX Model.

@entity1 @entity82 @entity1711 @entity3652 @entity4763 @entity1710 @entity1810 @entity35
PURPOSE: We sought to develop a reproducible @entity1810 injection technique to induce urethral @entity82 in the @entity35 urethra. MATERIALS AND METHODS: A total of 32 male @entity35 weighing 300 to 350 gm were anesthetized with @entity3652 / @entity4763 intraperitoneally. Using a 5 mm penoscrotal incision the @entity35 urethra was exposed. In the experimental group varying doses of @entity1810 (5, 10 and 25  g) were injected in each side of the urethral wall. Normal saline infiltration was used in the sham treated group. @entity35 were sacrificed 2 and 4 weeks following @entity1810 injection. Urethral specimens were stained with @entity1710 and @entity1711 , and Masson trichrome, and Western blot evaluations were performed. Normal and strictured urethral tissues from @entity1 were collected and evaluated in the same fashion. RESULTS: There was no evidence of urethral wall thickening or @entity82 in the sham treated group. Varied histological evidence of @entity82 was noted in all experimental groups. There was a significant increase in collagen type I expression 2 weeks after injection of 5, 10 and 25  g @entity1810 . Collagen type III expression was significantly increased 2 weeks after injecting 10 and 25  g of @entity1810 , which persisted to 28 days after injection. CONCLUSIONS: @entity1810 injection can successfully generate a reproducible @entity35 model of urethral spongiofibrosis. This technique is simple, inexpensive and reproducible. Our series is a proof of concept study. Additional studies in larger animals are needed to further confirm our findings.
