Reactive @entity26 species (ROS) from @entity1110 and XXXX oxidase modulate the cutaneous local heating response in healthy humans.

@entity11933 @entity274 @entity12824 @entity3624 @entity2178 @entity26 @entity1110 @entity208 @entity842 @entity224 @entity7743 @entity291 @entity30572
Local cutaneous heating produces vasodilation that is largely @entity842 (NO) dependent. We showed that @entity2178 ( @entity2178 ) attenuates this by an @entity2178 receptor, type 1 ( @entity7743 )-dependent mechanism that is reversible with the antioxidant ascorbate, indicating oxidative stress. Reactive @entity26 species (ROS) produced by @entity2178 employ @entity1110 and @entity3624 oxidase pathways. To determine whether these mechanisms pertain to skin, we measured cutaneous local heating with 10  M @entity2178 , using @entity12824 to inhibit @entity1110 oxidase and @entity208 to inhibit @entity3624 oxidase. We also inhibited @entity224 with @entity11933 , and @entity274 with ebselen. We heated the skin of the @entity291 in 8 healthy volunteers (24.5-29.9 yr old) to 42  C and measured local blood flow to assess the percentage of maximum cutaneous vascular conductance. We remeasured while perfusing @entity208 , @entity12824 , @entity30572 , and @entity11933 through individual microdialysis catheters. This was then repeated with @entity2178 combined with antioxidant drugs. @entity11933 and @entity12824 alone had no effect on the heat response. @entity208 enhanced the entire response (125% of heat alone), while ebselen suppressed the heat plateau (76% of heat alone). @entity2178 alone caused significant attenuation of the entire heat response (52%). When added to @entity2178 , @entity208 partially reversed the @entity2178 attenuation. Heat with ebselen and @entity2178 were similar to heat and @entity2178 ; ebselen only partially reversed the @entity2178 attenuation. @entity12824 and @entity11933 each partially reversed the attenuation caused by @entity2178 . This suggests that ROS, produced by @entity2178 via @entity1110 and @entity3624 oxidase pathways, modulates the response of skin to the application of heat, and thus contributes to the control of local cutaneous blood flow.
