Genetic and physical mapping of homologues of the virus resistance gene Rx1 and the cyst XXXX resistance gene @entity72542 in @entity415 .

@entity415 @entity72542 @entity2199 @entity74
Nine @entity74 ( @entity74 ) were identified in two diploid @entity415 clones (SH and RH), with a specific primer pair based on conserved motifs in the LRR domain of the @entity415 cyst @entity2199 resistance gene @entity72542 and the @entity415 virus X resistance gene Rx1. A modified AFLP method was used to facilitate the genetic mapping of the @entity74 in the four haplotypes under investigation. All nine @entity74 appeared to be located in the @entity72542 / Rx1 cluster on chromosome XII. Construction of a physical map using bacterial artificial chromosome (BAC) clones for both the @entity415 ssp. @entity415 and the @entity415 ssp. andigena haplotype of SH showed that the @entity74 are located within a stretch of less than 200 kb. Sequence analysis of the @entity74 revealed that they are highly similar (93 to 95%) to @entity72542 and Rx1. The sequence identities among all @entity74 range from 85 to 100%. Two pairs of @entity74 are identical, or nearly so (100 and 99.9%), with each member located in a different genotype. Southern-blot analysis on genomic DNA revealed no evidence for additional homologues outside the @entity72542 / Rx1 cluster on chromosome XII.
