Sialylation by b  -galactoside a  -2,6  -sialyltransferase and N  -glycans regulate cell adhesion and invasion in @entity1 XXXX .

@entity1 @entity689 @entity15695 @entity11297 @entity706 @entity41786 @entity5 @entity2108 @entity3
UNASSIGNED: The interaction between cell surface glycans and extracellular matrix  (ECM) including galectins is known to be closely associated with @entity5 cell adhesion, invasion and @entity3 . We analyzed the roles of cell surface sialylation or glycosylation in galectin or ECM  -mediated cell adhesion and invasion of @entity1 @entity706 cells. @entity689 from Arthrobacter ureafaciens  (AU) treatment resulted in reduction of cell adhesion to @entity41786 in @entity1 @entity2108  (H  -ALCL) which was established in our laboratory. The knockdown of b  -galactoside a  -2,6  -sialyltrans-ferase  ( @entity15695 ) by siRNA showed inhibition of @entity15695 expression in the cytoplasm of H  -ALCL cells on immunohistochemical findings, and showed dramatic enhancement of cell adhesion to @entity41786 . On the other hand, a  -2,3  -specific @entity689 treatment resulted in moderate enhancement of cell adhesion to @entity41786 . We performed chemically artificial modification of cell surface O  -glycans by treatment of benzyl 2  -acetamido  -2  -deoxy  -a  -D  -galactopyranoside  (Bz  -a  -GalNAc) in H  -ALCL. Cell adhesion to @entity41786 was enhanced by treatment of Bz  -a  -GalNAc suggesting that inhibition of elongation of O  -glycans may enhance cell adhesion to @entity41786 in H  -ALCL cells. On the other hand inhibition of elongation of N  -glycosylation by tunicamycin  (TM) resulted in inhibition of @entity11297  (L  -PHA) lectin  -binding activity and inhibited cell adhesion to @entity41786 , laminin and fibronectin. @entity689 treatment enhanced cell adhesion to laminin, and knockdown of @entity15695 resulted in enhancement of cell adhesion to laminin, but not to fibronectin, collagen type  1  and  4. @entity41786 pre  -treatment dramatically enhanced cell adhesion to laminin and @entity689 treatment also enhanced cell adhesion to laminin in combination with @entity41786 . Rho inhibitor, C3  -transferase pre  -treatment resulted in inhibition of cell invasion to @entity41786 . Phosphatidylinositol  3  -phosphate kinase  (PI3K) inhibitor, wortmannin inhibits the cell invasive capacity to @entity41786 . @entity689 treatment induces growth inhibition of @entity706 cells by @entity41786 .
