The @entity406 -induced expression of XXXX peptide and neurokinin receptor messenger RNAs in rat sensory ganglia and spinal cord is modulated by opiate preadministration.

@entity836 @entity16325 @entity406 @entity158 @entity1286 @entity16423 @entity4756
@entity4756 peptides such as substance P and @entity16423 have been widely studied as mediators of @entity158 transmission. The expression of neurokinin-1 and neurokinin-3 receptor messenger RNAs in the spinal cord is increased following intense nociception. The opiate ligands @entity1286 and @entity836 alter behavioral responses to @entity406 -induced @entity158 and alter evoked substance P release. This study investigated whether these opiates similarly alter the expression of substance P-, @entity16423 -, @entity16325 - and neurokinin-3 receptor-encoding messenger RNAs in spinal systems following @entity406 -induced nociception. Expression levels of various messenger RNAs were quantitated using solution hybridization-nuclease protection assays. Six hours after hindpaw treatment, the levels of substance P-encoding preprotachykinin messenger RNA in the lumbar dorsal root ganglia and @entity16423 , @entity16325 and neurokinin-3 receptor messenger RNAs in the lumbar dorsal horn were increased by approximately two-fold as compared to sham-treated controls. Pretreatment with @entity836 resulted in a further increase in the nociception-induced substance P messenger RNA expression in the dorsal root ganglia; preprotachykinin messenger RNA expression was not affected by @entity1286 . Nociception-induced @entity16325 messenger RNA expression in the dorsal horn was blocked by @entity1286 , but was not affected by @entity836 . Both @entity1286 and @entity836 blocked the @entity406 -induced increases in @entity16423 and neurokinin-3 receptor messenger RNA levels. Increased @entity16423 messenger RNA expression may reflect increased @entity16423 turnover in spinal interneurons activated by nociception. Neurokinin-3 receptor messenger RNA expression levels varied closely with, and thus may be regulated by, the levels of @entity16423 messenger RNA in the same regions. The results of this study indicate that pretreatment with opiate ligands modulates the expression of @entity4756 peptide and neurokinin receptor encoding mRNAs in spinal systems following a peripheral chemogenic inflammatory stimulus. Thus, endogenous opioid systems may be involved in activity-induced changes in the expression of spinal @entity4756 peptides and neurokinin receptors.
