Airway responsiveness to @entity2656 in XXXX is associated with chymase-positive mast cells and @entity32 .

@entity2656 @entity32 @entity1 @entity565 @entity26135 @entity1224 @entity24373
BACKGROUND: @entity1224 ( @entity1224 ) to inhaled @entity2656 is associated with indirect markers of mast cell activation and @entity32 . It is unknown how @entity1224 to @entity2656 relates to mast cell phenotype, mast cell function and measures of @entity32 in airway tissue. We compared the number and phenotype of mast cells, mRNA expression of mast cell-associated genes and number of eosinophils in airway tissue of subjects with @entity565 and healthy controls in relation to @entity1224 to @entity2656 . METHODS: @entity1224 to inhaled @entity2656 was measured in 23 non-smoking, corticosteroid-free asthmatic individuals and 10 healthy controls. Mast cells and eosinophils were identified in mucosal biopsies from all @entity1 . Mast cells were divided into phenotypes based on the presence of chymase. mRNA expression of mast cell-associated genes was measured by real-time PCR. RESULTS: The proportion of submucosal MCTC was higher in asthmatic individuals with @entity1224 to @entity2656 compared with asthmatic individuals without @entity1224 (median: 40.3% vs. 18.7%, P  =  0.03). Increased submucosal MCTC numbers were associated with increased levels of mRNA for @entity24373 ( @entity24373 ) and @entity26135 in asthmatics. Reactivity to @entity2656 correlated significantly with eosinophils in submucosa (r(s): 0.56, P  =  0.01). CONCLUSION: @entity1224 to inhaled @entity2656 is associated with an altered submucosal mast cell profile in asthmatic individuals. This mast cell profile is associated with increased levels of @entity24373 and @entity26135 . The degree of @entity1224 to @entity2656 is correlated with the degree of @entity32 in the airway submucosa.
