Type II and type IX collagen transcript isoforms are expressed during XXXX testis development.

@entity462 @entity853 @entity19 @entity1863 @entity15137 @entity430 @entity54693 @entity32942 @entity72648 @entity72649
Mutations in the transcription factor @entity430 give rise to @entity32942 , a syndrome characterized by @entity853 and XY sex reversal. @entity430 is expressed at sites of chondrogenesis and in the developing testis, and, thus, it plays a role in two overtly different pathways of differentiation. Previous studies have identified the gene for type II collagen, @entity15137 , as a target of @entity430 in @entity19 chondrocytes and implicated @entity72648 as a @entity430 target in testis. Using differential expression analysis combined with reverse transcription-polymerase chain reaction and whole-mount in situ hybridization, we have identified nonchondrocytic collagen transcript isoforms that are expressed in the early male @entity19 gonad. Male-specific, gonadal expression of nonchondrocytic @entity15137 was first seen at 11.5 days postcoitum (dpc) and was undetectable by 13.5 dpc. This was accompanied by increasing expression of nonchondrocytic @entity54693 , @entity72649 , and @entity72648 , first detected at 11.5 dpc. Expression was analyzed in testes that had been depleted of germ cells by the cytotoxic drug @entity1863 . These studies showed @entity72648 and @entity15137 to be expressed in Sertoli cells within the developing testis cords. Nonchondrocytic type II collagen contains a @entity462 -rich domain that has been shown to bind members of the transforming growth factor beta superfamily of signaling molecules. Thus, this interaction may play a role in the morphogenesis and differentiation of the testis.
