Serum miR-338-5p, Soluble XXXX , Allo-Antibodies, and Renal Transplantation.

@entity71611 @entity1531 @entity8410
BACKGROUND: The objective of the study was to explore the expression features of serum miR-338-5p and soluble @entity8410 (sBAFF) in renal transplant recipients. METHODS: Follow-up renal transplant recipients (n  = 49) were enrolled in this study (male/female: 38/11). Healthy volunteers were controlled; 2  mL of peripheral blood from each subject was collected. Total RNA was extracted from serum by use of the miRNeasy Serum/Plasma Kit (QIAGEN), and miR-338-5p was amplified by means of quantitative real-time reverse transcriptase-polymerase chain reaction. sBAFF was detected by means of enzyme-linked immunoassay. LABScreen Mix ( @entity71611 ) (One Lambda) was used to test the level of anti-human leukocyte antigen (HLA) I antibody (Ab), anti-HLA II Ab, and anti-major histocompatibility complex class I chain-related A ( @entity1531 ) Ab. All data are shown as mean    SD and were analyzed by use of SPSS software 17.0. RESULTS: Compared with healthy volunteers, serum miR-338-5p in recipients was statistically downregulated (2.79    2.5 versus 0.09    0.12, P  < .001); sBAFF in recipients was significantly upregulated (1321    950  pg/mL versus 534    327  pg/mL, P  < .01); serum anti-HLAII Ab, anti- @entity1531 Ab, and anti-HLA+ @entity1531 Abs all statistically increased in recipients (P  < .05). Spearman correlation analysis showed that miR-338-5p was significantly negatively correlated with sBAFF (r  =  -0.51, P  < .001) and anti-HLA II antibody with mean fluorescence intensity value >1000 (r  =  -0.322, P  < .05). Analysis results also suggested that sBAFF was significantly negatively correlated with anti- @entity1531 Ab, with mean fluorescence intensity value >1000 (r  =  -0.579, P  < .05). CONCLUSIONS: miR-338-5p is closely correlated with the procedure of renal allograft antibody-mediated rejection.
