Sensitization to @entity1205 in @entity263 cells by @entity3641 and XXXX .

@entity263 @entity5 @entity3089 @entity584 @entity1759 @entity31258 @entity11707 @entity3092 @entity342 @entity3641 @entity3096 @entity1205 @entity4420
Chemotherapy with @entity1205 ( @entity4420 ) is a standard treatment for metastatic and castration-resistant @entity263 . However, chemoresistance and side effects of @entity4420 limit its clinical success. We investigated whether natural products @entity3641 ( @entity3641 ) and @entity3092 (Q), a @entity3096 from apples and onions, will enhance the efficacy of @entity4420 in @entity342 -independent (AI) @entity263 cells. Two cell lines including LAPC-4-AI and PC-3 were treated in vitro with 40  M of @entity3089 (EGCG), 5  M of Q, 2 or 5 nM of @entity4420 alone or in combination. The mixture of EGCG+Q+ @entity4420 increased the antiproliferative effect by threefold in LAPC-4-AI cells and eightfold in PC-3 cells compared to @entity4420 alone. EGCG, Q and @entity4420 in combination significantly enhanced cell cycle arrest at G2/M phase and increased apoptosis in both LAPC-4-AI and PC-3 cells compared to @entity4420 alone. The mixture increased the inhibition of PI3K/ @entity584 and the signal transducer and activator of transcription (Stat) 3 signaling pathways compared to @entity4420 alone, and decreased the protein expression of @entity31258 . In addition, the combination with EGCG and Q increased the inhibition of @entity5 cell invasion and colony formation in both LAPC-4-AI and PC-3 cells compared to @entity4420 alone, and decreased the percentage of @entity1759 (+)/ @entity11707 (-) stem-like LAPC-4-AI cells. In summary, @entity3641 and Q enhanced the therapeutic effect of @entity4420 in castration-resistant @entity263 cells through multiple mechanisms including the down-regulation of chemoresistance-related proteins. This study provides a novel therapeutic modality to enhance the efficacy of @entity4420 in a nontoxic manner.
