Characterization of XXXX and @entity10015 in blood, tissue and cell lines of @entity213 @entity1 and their impact on cell proliferation and migration.

@entity1 @entity696 @entity5 @entity10015 @entity6991 @entity213
PURPOSE: @entity213 ( @entity213 ) is one of the most common @entity5 worldwide with an unchanged 5-year survival rate during the last decade. To detect reliable prognostic markers and improve @entity1 ' outcome in future, the aim of our study was to detect differences in microRNA (miRNA; @entity696 ) expression profile and further on to analyze the functional role of selected miRNAs. METHODS: Blood samples from @entity213 @entity1 and sex- and age-matched healthy volunteers were analyzed by microarrays and validated by quantitative real-time PCR. Data were compared with @entity5 tissue results and all findings were correlated with clinical parameters. Additionally, the proliferation and migration potential of two cell lines transfected with miRNA mimics and inhibitors for @entity6991 and @entity10015 were examined. RESULTS: Initial analysis of blood samples showed no significant differences between the miRNA profile of @entity213 @entity1 and healthy controls (p  >  0.05). Interestingly, down-regulation of @entity6991 and @entity10015 in blood of @entity1 correlated with the occurrence of distant metastasis regarding @entity5 @entity1 only (p  =  0.023 and p  =  0.028, respectively). Additionally, our investigations in tissue samples revealed a lower expression of @entity10015 in @entity5 cells (p  =  0.003) and a correlation with higher cT-classification for down-regulation of @entity6991 (p  =  0.005). Moreover, functional assays demonstrated that inhibition of @entity6991 and @entity10015 promoted dramatically proliferation and migration potential, whereas transfection of both mimics had an inhibitory effect. CONCLUSIONS: Characterizing the expression of @entity6991 and @entity10015 and their functional role in @entity5 biology underlined significantly their proliferation and migration potential suggesting relevance as potential prognostic markers in @entity213 .
