Isolation of three crystalline proteins from beef liver after partial purification by XXXX fractionation.

@entity2196 @entity5723 @entity1324
The isolation of three proteins in crystalline form from ground beef liver is described. These proteins are FTBL protein (Arch. Biochem. Biophys. 188, 251-265 (1978), crotonase, and @entity2196 . Crotonase is isolated by crystallization from a 32% @entity1324 extract of the ground liver. FTBL protein and @entity2196 can subsequently be isolated from the same extract. For optimal yield and ease of isolation, FTBL protein is isolated from a 46.5% @entity1324 extract from which @entity2196 can subsequently be crystallized by dialysis. The isolation of FTBL protein as well as the isolation of @entity2196 involves a preliminary fractional precipitation and solution before crystallization can be achieved. @entity5723 can be substituted for @entity1324 in the isolation of the above three proteins and in the case of @entity2196 , results in an exceptionally high yield. Methods for the recrystallization of the proteins are presented and the role of organic solvents in recrystallization is discussed.
