[The role of XXXX protein 70 and its autoantibody in experimental autoimmune @entity389 ].

@entity1 @entity1457 @entity840 @entity9487 @entity389
OBJECTIVE: To examine the effect of antigen stimulation on induction of the @entity1457 protein 70(HSP70) in the @entity840 cochlea and to explore the role of HSP70 and its autoantibody in the pathogenesis and diagnosis of @entity389 ( @entity389 ). METHODS: To establish the animal model of @entity389 , homologous crude inner ear antigen was used to immunize the @entity840 . The expression of HSP70 was detected by immunocytochemistry and the relative staining densities were quantified with a light microscope image analysis system. The autoantibodies against @entity9487 in the plasma of animals were tested by Western blot using purified recombinant @entity1 @entity9487 as antigen. RESULTS: HSP70 presented in the normal @entity840 cochlea at a lower level. After immunological challenge, the levels of HSP70 immunoreactivity in the immunized animals were significantly increased as compared with control animals. Optical densities of cochleae of immunized animals were significantly greater than those of animals in the control group (P < 0.01). Most of the immunized animals had developed autoantibodies against @entity9487 . The incidence of autoantibody in the experimental group was significantly different (P < 0.01) from the control group. CONCLUSION: Antigen stimulation could lead to an increase in the synthesis of HSP70 in the @entity840 cochlea. The detection of autoantibody against @entity9487 might have significance in the diagnosis of @entity389 .
