Study of idiotopic suppression induced by anti-cross-reactive idiotype monoclonal antibody in the anti- XXXX antibody response.

@entity72324 @entity59752 @entity19 @entity535 @entity72323 @entity17118 @entity12808
A/J @entity19 immunized with @entity59752 coupled to keyhole limpet hemocyanin produce antibodies expressing a cross-reactive idiotype (CRIA). The pretreatment of A/J mice with anti-idiotypic polyclonal or monoclonal antibody directed against the major cross-reactive idiotype (CRIA) borne by @entity59752 -specific antibody can lead to idiotypic suppression. In this study, we investigate this idiotypic suppression by using four mAb2 ( @entity72323 ) recognizing distinct idiotopes whose expression is related to the presence of particular gene segments of the heavy chain V region. 2D3 expression has been related to the presence of some @entity535 in the @entity72324 region of the VH gene segment derived from the germ line VH IdCR11. So far, the latter is the only germ-line gene coding for CRIA+ antibody that has been identified in the A/J genome. E4 and H8 expression has been related to the use of a particular D segment, whereas E3 expression has been attributed to certain combinations of D and @entity12808 segments. Therefore, we might expect independent regulation of the expression of those various idiotopes in relation to the mechanism of gene recombination. Indeed, we observed that 2D3-suppressed A/J @entity19 still produce the three other idiotopes, suggesting the recombination of those particular D and J segments with a different VH gene. Such a gene has been identified in the genome of BALB/c @entity19 . A/J @entity19 pretreated with one of the other three mAb2 are generally cosuppressed for the expression of E4, H8, and E3, but they still produce 2D3+ antibody. In this case, the IdCR11 VH germ-line gene is most probably recombined with different D and J segments. Molecular evidence for the existence of such molecules has also been presented in the literature. So our serologic data on idiotopic suppression in the @entity17118 system can be compared with recent data provided by molecular genetics.
