Metabolomics data reveal a crucial role of cytosolic @entity1160 synthetase 1;1 in coordinating metabolic balance in XXXX .

@entity1522 @entity1568 @entity7300 @entity1160 @entity505 @entity6626 @entity164 @entity3104 @entity579 @entity3363
@entity579 plants grown in paddy fields preferentially use @entity3104 as a source of inorganic @entity1568 . @entity1160 synthetase (GS) catalyses the conversion of @entity3104 to @entity1160 . Of the three genes encoding cytosolic GS in @entity579 , OsGS1;1 is critical for normal growth and grain filling. However, the basis of its physiological function that may alter the rate of @entity1568 assimilation and @entity505 metabolism within the context of metabolic networks remains unclear. To address this issue, we carried out quantitative comparative analyses between the metabolite profiles of a @entity579 mutant lacking OsGS1;1 and its background @entity164 ( @entity164 ). The mutant plants exhibited severe retardation of shoot growth in the presence of @entity3104 compared with the @entity164 . Overaccumulation of free @entity3104 in the leaf sheath and roots of the mutant indicated the importance of OsGS1;1 for @entity3104 assimilation in both organs. The metabolite profiles of the mutant line revealed: (i) an imbalance in levels of @entity3363 , @entity1522 and metabolites in the @entity6626 cycle, and (ii) overaccumulation of secondary metabolites, particularly in the roots under a continuous supply of @entity3104 . Metabolite-to-metabolite correlation analysis revealed the presence of mutant-specific networks between @entity7300 and other primary metabolites in the roots. These results demonstrated a crucial function of OsGS1;1 in coordinating the global metabolic network in @entity579 plants grown using @entity3104 as the @entity1568 source.
