Putative @entity5 in primary XXXX .

@entity1 @entity4730 @entity5 @entity3544 @entity219 @entity2 @entity4910 @entity356
PURPOSE: Kr  ppel-like factor 4 ( @entity4730 ) is a @entity219 -finger protein that plays important roles in stem cells and the development of @entity356 . However, the role of @entity4730 in primary @entity2 is unknown. The purpose of this study is to determine possible roles of @entity4730 in @entity2 . EXPERIMENTAL DESIGN: The @entity4730 expression in primary @entity2 tissues and case-matched normal lung tissues were determined by protein and mRNA analyses. The effects of @entity4730 on cell proliferation, clonogenic formation, and cell cycle progression were determined in cultured @entity2 cells or bronchial epithelial cells after enforced @entity4730 overexpression or small interfering RNA knockdown. The in vivo antitumor activity of @entity4730 was evaluated by using stably transfected @entity2 cells and by adenovector-mediated gene delivery. The effect of @entity4730 in regulating @entity3544 and @entity4910 was also evaluated. RESULTS: @entity4730 protein and mRNA levels were dramatically decreased in most primary @entity2 compared with in case-matched normal lung tissues. Enforced expression of @entity4730 resulted in marked inhibition of cell growth and clonogenic formation. The @entity5 -suppressive effect of @entity4730 was associated with its role in up-regulating @entity3544 and down-regulating @entity4910 , leading to cell cycle arrest at the G(1)-S checkpoint. Knockdown of @entity4730 promoted cell growth in immortalized @entity1 bronchial epithelial cells. The enforced expression of @entity4730 gene to @entity2 cells by ex vivo transfection or adenovector-mediated gene transfer suppressed @entity5 growth in vivo. CONCLUSIONS: Our results suggest that @entity4730 plays an important role in suppressing the growth of @entity2 .
