Th17 cytokines differentiate XXXX from @entity28 -associated type 2 @entity6 and promote @entity310 production.

@entity1 @entity32 @entity6 @entity28 @entity310 @entity784 @entity1198 @entity4854
OBJECTIVE: @entity784 plays pivotal roles in @entity28 -associated type 2 @entity6 (T2DM). The identification of dominant sources of @entity784 in @entity1 remains a significant gap in understanding disease pathogenesis. It was hypothesized that cytokine profiles from circulating T cells identify T cell subsets and T cell cytokines that define T2DM-associated @entity32 . METHODS: Multiplex analyses were used to quantify T cell-associated cytokines in aCD3/aCD28-stimulated PBMCs, or B cell-depleted PBMCs, from subjects with T2DM or BMI-matched controls. Cytokine measurements were subjected to multivariate (principal component and partial least squares) analyses. Flow cytometry detected intracellular @entity310 in multiple immune cell subsets in the presence/absence of antibodies that neutralize T cell cytokines. RESULTS: T cell cytokines were generally higher in T2DM samples, but Th17 cytokines are specifically important for classifying individuals correctly as T2DM. Multivariate analyses indicated that B cells support Th17 @entity32 in T2DM but not control samples, while monocytes supported Th17 @entity32 regardless of T2DM status. Partial least squares regression analysis indicated that both Th17 and @entity1198 cytokines impact %HbA1c. CONCLUSIONS: Among various T cell subsets, Th17 cells are major contributors to @entity32 and @entity4854 and are uniquely supported by B cells in @entity28 -associated T2DM.
