Genotypic and phenotypic characterization of side population of XXXX cell lines.

@entity1 @entity3375 @entity5956 @entity5439 @entity5 @entity5440 @entity13037 @entity21507 @entity356
The side population (SP) of @entity5 cell lines shares characteristics with @entity5 stem cells. The objective of this study was to phenotypically and genotypically characterize the SP of @entity356 cell lines. SP cells were obtained from AGS and @entity1 @entity356 cells using @entity5956 staining and fluorescence-activated cell sorting. The cells were subsequently studied morphologically at cytology and immunocytochemistry, on the transcriptional level via gene array, and in cell culture using recultivation assays. Genes differentially expressed in SP cells were evaluated at immunohistochemistry in tissue samples from 486 @entity1 with @entity356 . The SP cells were smaller and rounder then non-SP cells. SP cells self-renewed in recultivation experiments and differentiated into SP and non-SP cells. Recultivated SP and non-SP cells exhibited distinct phenotypes in culture insofar as cell shape and colony formation. SP cells demonstrated increased levels of the stem cell markers @entity5439 and @entity5440 . Transcriptional analyses demonstrated that SP cells express genes that encode for stem cell properties including @entity13037 , @entity21507 , SMO, and @entity3375 . It was observed that @entity3375 and @entity13037 are differentially expressed in @entity1 @entity356 , and @entity13037 -positive @entity5 are associated with significantly shorter @entity1 survival. In conclusion, @entity1 @entity356 cell lines enclose a phenotypically and genotypically distinct cell population with @entity5 stem cell features. Phenotypic characteristics of this distinct cell population are also present in @entity356 tissue, and correlate with @entity1 survival.
