Development of macrophage and granulocyte colonies from XXXX peripheral blood.

@entity1 @entity29 @entity53 @entity134 @entity2841 @entity7522
The presence of macrophage and granulocyte progenitor cells in the @entity1 peripheral blood enabled the establishment of colonies from this accessible tissue and obviated the need for bone marrow to achieve this task. We have developed a method of obtaining reproducible growth of macrophage/granulocyte colonies from @entity1 peripheral blood. Colonies of macrophages and granulocytes were obtained by plating peripheral blood mononuclear cells (PBM) in @entity7522 containing medium in the presence of medium conditioned by nonstimulated PBM (CM). At early stages of colony growth both macrophage and granulocyte colonies were detected while following 20-25 days in culture all colonies tested revealed monocyte-macrophage morphology. To obtain higher numbers of colonies, we tested different cell sources, different CM preparations and the effect of @entity134 hormones on colony development. We found that the mononuclear cells obtained from cord blood (CB) or from some @entity1 with @entity29 yielded much higher numbers of colonies than PBM from normal individuals. Colony development from these two sources did not depend on an external source of @entity2841 ( @entity2841 ) but was augmented as a result of @entity2841 supplementation. CM obtained from CB mononuclear cells as well as supernatants from some @entity1 monoblastic cell lines were similar in their @entity2841 activity to CM from normal PBM and made possible the development of macrophage/granulocyte colonies. Higher numbers of colonies were induced by including physiological concentrations of @entity53 in the culture medium, in the absence of external sources of @entity2841 . The system described above enabled the analysis of cloned macrophages and their circulating progenitor cells as well as the assay of different preparations of @entity2841 .
