In vivo high-frequency, contrast-enhanced ultrasonography of @entity2184 in XXXX : imaging features and histopathologic correlations.

@entity2880 @entity19 @entity5 @entity2184 @entity2093 @entity320
PURPOSE: To evaluate the usefulness of in vivo imaging of @entity2184 in @entity19 using high-frequency contrast-enhanced ultrasound (HF-CE-US) with 2D or 3D modes and to correlate the sonographic findings with histopathologic characteristics. METHODS: Fourteen 12-week-old C57BL6 @entity19 were inoculated into their right eyes with aliquots of 5   10(5)/2.5  L B16LS9 @entity320 cells and were randomly assigned to either of two groups. At 7 days after inoculation, @entity2093 in group 1 (n = 8) were imaged using HF-CE-US to determine the 2D @entity5 size and relative blood volume; eyes in group 2 (n = 6) were imaged by 3D microbubble contrast-enhanced ultrasound, and the @entity5 volume was determined. @entity5 burden was quantified in enucleated eyes by image processing software, and microvascular density was determined by counting @entity2880 factor-positive vascular channels. Ultrasound images were evaluated and compared with histopathologic findings. RESULTS: Using HF-CE-US, @entity320 were visualized as relatively hyperechoic regions. The intraobserver variability of sonographic measurements was 9.65%    7.89%, and the coefficient of variation for multiple measurements was 7.33%    5.71%. The correlation coefficient of sonographic volume or size and histologic area was 0.71 (P = 0.11) and 0.79 (P = 0.32). The relative blood volume within the @entity5 demonstrated sonographically correlated significantly with @entity5 vascularity (r = 0.83; P < 0.001). CONCLUSIONS: There was a positive linear correlation between @entity5 measurements and @entity5 burden in the @entity19 @entity320 model. Contrast-enhanced intensity corresponded with microvascular density and blood volume. HF-CE-US is a real-time, noninvasive, reliable method for in vivo evaluation of experimental intraocular @entity320 area and relative blood volume.
