Dynamics of regional brain metabolism and gene expression after XXXX in @entity19 .

@entity27619 @entity4798 @entity1885 @entity63 @entity19 @entity2500 @entity1977 @entity9830 @entity1188 @entity187 @entity432 @entity9829 @entity855
The evolution of @entity4798 during permanent occlusion of the middle cerebral artery (MCA) was studied in @entity19 using multiparametric imaging techniques. Regional protein synthesis and the regional tissue content of @entity855 were measured on adjacent cryostat sections at increasing intervals after @entity432 ranging from 1 hour to 3 days. The observed changes were correlated with the expression of the mRNA of @entity187 , @entity9830 , @entity9829 , and @entity27619 , as well as the distribution of DNA double-strand breaks visualized by terminal deoxynucleotidyl transferase-mediated @entity1977 - @entity1885 nick end labelling (TUNEL). One hour after @entity2500 , the tissue volume with suppressed protein synthesis was distinctly larger than that in which @entity855 was depleted. With ongoing @entity63 time, the @entity855 -depleted area gradually expanded and, within 1 day, merged with the region of suppressed protein synthesis. Expression of @entity187 mRNA occurred mainly in the penumbra (defined as the region of suppressed protein synthesis but preserved @entity855 ), peaking at 3 hours after @entity432 . Expression of the immediate-early genes @entity9829 , @entity9830 , and @entity27619 increased both in the penumbra and the periinfarct normal tissue already at 1 hour after @entity432 , with slightly different regional and temporal patterns for each of these genes. DNA fragmentations were clearly confined to neurons; they appeared after 1 day in the @entity1188 core (defined as the region of suppressed @entity855 ) and never were detected in the penumbra. The late appearance of TUNEL after @entity1188 had reached their final size and the absence in the penumbra points against a major pathogenetic role of apoptosis. @entity2500 in @entity19 thus produces a gradually expanding @entity1188 , the final size of which is heralded by the early inhibition of protein synthesis.
