@entity1968 inhibits XXXX via multiple genomic mechanisms.

@entity1204 @entity1 @entity34592 @entity588 @entity7379 @entity4879 @entity16142 @entity2454 @entity50029 @entity72286 @entity1968
Previous studies have indicated that @entity1968 (RA) may be therapeutic for @entity1204 . However, the downstream target genes and pathways triggered by ligand-activated @entity4879 ( @entity4879 ) in @entity1204 cells are largely unknown. In this study, @entity2454 , flow cytometry, and immunoblotting assays were used to assess the roles of RA and the RA agonist ( @entity50029 ) in the growth of @entity1204 cells. Illumina-based microarray expression profiling of endometrial Ishikawa cells incubated with and without @entity50029 for 1, 3, and 6h was performed. We found that both RA and @entity50029 markedly inhibited @entity1204 cell proliferation, while knockdown of @entity4879 could block @entity50029 inhibition. Knockdown of @entity4879 significantly increased proliferating cell nuclear antigen and @entity588 protein levels. Incubation of Ishikawa cells with or without @entity50029 followed by microarray expression profiling showed that 12768 genes out of 47296 gene probes were differentially expressed with significant P values. We found that 90 genes were the most regulated genes with the most significant P value (P<0.0001) using F-test. We selected four highly regulated genes with diverse functions, namely @entity16142 , @entity72286 , @entity7379 , and @entity34592 . Real-time PCR verified that @entity50029 highly regulated these genes, whereas chromatin immunoprecipitation-PCR assay demonstrated that ligand-activated @entity4879 interacted with the promoter of these genes in intact @entity1204 cells. @entity50029 also significantly altered 18 pathways including those related to cell growth, differentiation, and apoptosis. In conclusion, @entity50029 treatment of Ishikawa cells causes the differential expression of a number of @entity4879 target genes and activation of signaling pathways. These pathways could, therefore, mediate the carcinogenesis of @entity1 @entity1204 .
