[Experimental studies on the antitumor immunity induced by total XXXX RNA- transfected dendritic cells].

@entity1 @entity5 @entity19 @entity189 @entity98
OBJECTIVE: The efficiency of antitumor immunity induced by dendritic cells (DCs) transfected with total RNA of @entity189 cells was explored. METHOD: DCs, induced from @entity1 peripheral blood mononuclear cells, were transfected with total RNA of @entity189 cells. The specific antitumor immunity of cytotoxic T Lymphocytes (CTLs) that were actived by RNA-transfected DCs were detected by @entity98 methods in vitro. In vivo, antitumor-specific CTLs were subcutaneously injected into the @entity19 previously. After 7 days, the @entity189 cells were seeded and the @entity5 occurrence rate was observed. @entity5 -loaded @entity19 were treated by specific CTLs once (the treated group) or twice (the retreated group). The growth of the implanted @entity5 was observed too. RESULT: DCs that transfected with @entity5 RNA can significantly active CTLs which induced antitumor-specific immune response against @entity189 in vitro. In vivo, the @entity5 occurrence rate of the treatment group was predominantly reduced compared with that of the control (P < 0.01). The implanted @entity5 size of the treated and retreated groups were both significantly reduced (P < 0.05, P < 0.01) compared with the control too, especially the retreated ones (P < 0.05). CONCLUSION: The @entity5 RNA loaded DCs can significantly active CTLs and the antitumor specific CTLs can both induce antitumor specific immune response against @entity189 in vitro and inhibit the growth of the implanted @entity5 in vivo.
