Determination of XXXX in Powdered Milk Using Direct Analysis in Real Time Quadrupole Time-of-Flight Tandem Mass Spectrometry.

@entity1626 @entity6061 @entity15 @entity31769
UNASSIGNED: The direct analysis in real time (DART) ionization source coupled with quadrupole time-of-flight tandem mass spectrometry (Q-TOF @entity15 / @entity15 ) system has the capability to desorb analytes directly from samples without sample cleanup or chromatographic separation. In this work, a method based on DART/Q-TOF @entity15 / @entity15 has been developed for rapid identification of @entity31769 ( @entity6061 ) present in powdered milk. Simple sample extraction procedure employing @entity1626 -water (80:20, v/v) mixture was followed by direct, high-throughput determination of sample extracts spread on a steel mesh of the transmission module by mass spectrometry under ambient conditions. The method has been evaluated for both qualitative and quantitative analysis of @entity6061 in powdered milk. Variables including experimental apparatus, DART gas heater temperature, sample presentation speed, and vacuum pressure were investigated. The quantitative method was validated with respect to linearity, sensitivity, repeatability, precision, and accuracy by using external standards. After optimization of these parameters, a limit of detection (LOD) of 100  g kg(-1) was obtained for @entity6061 with a linear working range from 100 to 10000  g kg(-1) and a satisfactory correlation coefficient (R(2)) of 0.9997. Good recovery (80.08%-106.47%) and repeatability (RSD   =   3.0%-5.4%) were achieved for @entity6061 . The DART/Q-TOF @entity15 / @entity15 -based method provides a rapid, efficient, and powerful scheme to analyze @entity6061 in powdered milk with limited sample preparation, thus reducing time and complexity of quality control. Graphical Abstract    .
