Immunohistochemical study of the upper surface layer in XXXX mandibular condylar cartilage.

@entity35 @entity1604 @entity13461 @entity4023
Both @entity4023 and fibronectin localizations were examined in the upper surface layer of @entity35 mandibular condylar cartilages by immunohistochemical techniques. Their delicate structure was successfully preserved by preparation procedures of joint condyles with disks. @entity1604 -fixed cartilaginous tissues were cut in a cryostat, and cryosections were analyzed using streptavidin-peroxidase and indirect immunofluorescence methods. Another immunogold method with conventional preparation procedures and a quick-freezing method was performed for their ultrastructural analyses. Both @entity4023 -binding protein and anti-fibronectin antibody were used to localize @entity4023 and fibronectin in the mandibular condylar cartilage, respectively. Some cryosections were pre-treated with hyaluronidase and @entity13461 before such labeling. The upper surface layer was composed of double laminar structures. One bordered with the cartilage matriceal surface, which was positive for fibronectin. The @entity4023 was localized over the fibronectin layer. Therefore, the @entity4023 in vivo was bound with fibronectin in the cartilaginous matrix, performing lubrication for the mandibular joint movement.
