Proteome catalog of XXXX captured during pathogenesis in @entity3505 .

@entity3505 @entity37918 @entity71155 @entity15
@entity37918 is an economically important pathogen of @entity3505 . However, the molecular basis of pathogenicity on @entity3505 is still poorly understood. Here, we present a global survey of the proteins secreted by this fungus in the apoplast of resistant (cv. Shafir) and susceptible (cv. Obelisk) @entity3505 cultivars after inoculation with reference @entity71155 . The fungal proteins present in apoplastic fluids were analyzed by gel electrophoresis and by data-independent acquisition liquid chromatography/mass spectrometry (LC/ @entity15 (E)) combined with data-dependent acquisition LC- @entity15 / @entity15 . Subsequent mapping mass spectrometry-derived peptide sequence data against the genome sequence of strain IPO323 identified 665 peptides in the @entity15 (E) and 93 in the LC- @entity15 / @entity15 mode that matched to 85 proteins. The identified fungal proteins, including cell-wall degrading enzymes and proteases, might function in pathogenicity, but the functions of many remain unknown. Most fungal proteins accumulated in cv. Obelisk at the onset of necrotrophy. This inventory provides an excellent basis for future detailed studies on the role of these genes and their encoded proteins during pathogenesis in @entity3505 .
