Growth and function of thirty-four XXXX benign and malignant thyroid xenografts in untreated @entity19 .

@entity1 @entity1496 @entity2743 @entity5 @entity19 @entity189 @entity3745 @entity652 @entity259 @entity1374
Tissue was taken from 16 @entity1 with @entity1374 (10 nontoxic nodular colloid goiter, two @entity652 , one @entity652 , one @entity1496 -induced @entity2743 , 2 @entity2743 ) and 18 @entity1 with @entity5 [seven papillary, five follicular, five undifferentiated (anaplastic), and one @entity189 ] and was xenotransplanted into the flanks of 124 syngeneic female BALB/c-nu/nu @entity19 6 weeks of age. Subsequently, without any further treatment, serum levels of @entity3745 (TG), T3, T4, and thyroid-stimulating hormone were determined by radioimmunoassay at 4 or 5 weeks posttransplantation and at the end of the experimental time period of 4 months. All animals were autopsied. The grafts were examined by light microscopy and TG immunohistochemistry. Morphologically, the grafts of benign and @entity5 showed features overall identical to the original tissue. Conversely, nontoxic nodular colloid goiter and @entity2743 grafts revealed a transformation to normofollicular structures. All benign thyroid grafts showed a stationary growth, as did most differentiated @entity259 . @entity5 take rates in differentiated and in @entity189 were 15%, and in @entity189 , 100%. In the @entity5 grafts, a correlation between resting phase (period until progressive @entity5 ) and survival time of the corresponding @entity1 was disclosed. All @entity1 whose @entity5 were not taken by @entity19 are still alive and show no signs of progressive @entity5 at 9 to 34 months after surgery. All but one @entity1 with @entity5 revealing positive @entity5 take died within 3 months (resting phase, 3 weeks) or one year (resting phase, 7 to 14 weeks) after surgery. Integrity of hormonal function in benign and malignant xenografts at 4 months posttransplantation could be shown by significantly higher T3 and T4 serum concentrations in animals with benign thyroid tissues (T3, 1.69 +/- 0.13 nmol/liter; T4, 45.69 +/- 2.09 nmol/liter; S.E.) as compared to controls without grafted tissue [T3, 1.29 +/- 0.10 nmol/liter (p less than 0.05); T4, 33.39 +/- 2.71 nmol/liter (p less than 0.05)] and by increased TG serum concentrations in animals receiving benign (TG, 2.70 +/- 1.39 ng/ml) or malignant (e.g., @entity189 , 34.44 +/- 13.83 ng/ml; controls, 0.30 +/- 0.02 ng/ml) thyroid tissue. Thus, we conclude that benign and malignant thyroid xenografts in the nude @entity19 maintain full morphological and, regarding T3, T4, and TG serum levels, functional integrity for at least 4 months after transplantation.
