Effect of restriction of placental growth on expression of IGFs in fetal XXXX : relationship to fetal growth, circulating IGFs and binding proteins.

@entity742 @entity45339 @entity9419 @entity9418 @entity413 @entity45338 @entity4095
To determine whether tissue production of the IGFs is altered when fetal growth is retarded, @entity45338 mRNAs were measured in tissues of fetal @entity742 subjected to placental restriction and the relationships between IGF gene expression, circulating IGF protein and fetal growth were examined. The majority of potential placental attachment sites were surgically removed from the uterus of 12 non-pregnant ewes to restrict placental size in a subsequent pregnancy. Blood and tissues were collected at 121 days of gestation (term = 150) in 12 fetuses with restricted placental size and eight normal fetuses. @entity9418 and @entity9419 mRNA was detected by solution hybridization/ribonuclease protection assay in placenta and all fetal tissues studied. @entity9418 mRNA was most abundant in skeletal muscle and liver and @entity9419 mRNA was highest in kidney and lung. Restriction of placental size reduced fetal weight by 17% and reduced the @entity4095 (18%) and @entity413 concentration (23%) of fetal blood. Placental restriction also reduced @entity9418 mRNA in fetal muscle (P < 0.002), lung (P < 0.05) and kidney (P < 0.01) but had no significant effect on @entity9419 mRNA in any tissue. @entity9418 mRNA in fetal liver, kidney and skeletal muscle correlated positively with the concentration of @entity9418 protein in fetal blood (P < 0.01). There was no relationship between the concentration of @entity9419 protein in fetal blood and @entity9419 mRNA in any fetal tissue examined. The concentration of @entity45339 ( @entity45339 ) in fetal arterial blood plasma measured by RIA correlated positively with fetal weight and with plasma @entity9418 .(ABSTRACT TRUNCATED AT 250 WORDS)
