XXXX glutaredoxin 5-deficient cells subjected to continuous oxidizing conditions are affected in the expression of specific sets of genes.

@entity26301 @entity70877 @entity1885 @entity70876 @entity22540 @entity37082 @entity599 @entity375 @entity19624 @entity68
The @entity375 @entity70876 gene codes for a mitochondrial glutaredoxin involved in the synthesis of @entity68 / @entity599 clusters. Its absence prevents respiratory growth and causes the accumulation of @entity68 inside cells and constitutive oxidation of proteins. Null Deltagrx5 mutants were used as an example of continuously oxidized cells, as opposed to situations in which oxidative stress is instantaneously caused by addition of external oxidants. Whole transcriptome analysis was carried out in the mutant cells. The set of genes whose expression was affected by the absence of @entity70876 does not significantly overlap with the set of genes affected in respiratory petite mutants. Many @entity22540 -dependent genes involved in @entity68 utilization that are up-regulated in a frataxin mutant were also up-regulated in the absence of @entity70876 . @entity70877 is another @entity22540 -dependent gene induced both upon @entity68 deprivation and in Deltagrx5 cells; this links @entity68 and @entity1885 metabolism. Other genes are specifically affected under the oxidative conditions generated by the @entity70876 mutation. One of these is @entity26301 , which codes for a homologue of the @entity19624 kinase. Cells lacking @entity26301 and @entity70876 are hypersensitive to oxidative stress caused by external agents and exhibit increased protein oxidation in relation to single mutants. This in turn points to a role for @entity26301 in protection against oxidative stress. The genes of the @entity37082 regulon, which are involved in respiratory metabolism, are down-regulated in Deltagrx5 cells. This effect is suppressed by @entity37082 overexpression. Inhibition of respiratory metabolism during continuous moderately oxidative conditions could be a protective response by the cell.
