MicroRNA-222 Controls @entity1 @entity788 Cell Line Capan-2 Proliferation by XXXX Targeting.

@entity1 @entity788 @entity10081 @entity5 @entity993 @entity983 @entity8755 @entity10074
@entity788 ( @entity788 ) is one of the most common @entity5 and has a poor prognosis due to late diagnosis and ineffective therapeutic multimodality. MicroRNAs (miRNAs, miRs) are a group of non-coding, small RNAs with active biological activities. In our investigation, @entity1 @entity788 cell line Capan-2 were transfected with @entity10074 mimics, inhibitors or their negative controls. Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8), @entity10081 incorporation assay and cell cycle determination by flow cytometry. @entity10074 and putative target gene expression levels including @entity993 , @entity8755 and @entity983 were determined using quantitative reverse transcription polymerase chain reactions and Western blotting. Our results showed that @entity10074 could lead to increased vitality and proliferative rate of @entity1 cells, and also higher S-phase and lower G1-phase of cell cycle. Further, we found @entity8755 at protein level, but not @entity993 nor @entity983 , was regulated by @entity10074 in @entity1 cells. Finally, we co-transfected @entity10074 inhibitor and @entity8755 si-RNA into @entity1 cells, and found that proliferation-suppressing effects of @entity10074 inhibitor on @entity1 cells could be partially reversed by silencing @entity8755 . Our results indicate that @entity10074 controls @entity1 cell proliferation by targeting @entity8755 . This study provides a novel idea for developing effective therapeutic strategy for @entity788 @entity1 through inhibiting @entity10074 .
