Oxidant-mediated ciliary dysfunction. Possible role in XXXX .

@entity274 @entity26 @entity273 @entity1030 @entity35
The effects of reactive species of @entity26 on the airway are not well known. This study examined the effects of @entity273 ( @entity274 ) on the structure and function of the airway epithelium. Tracheal rings were prepared from 200 g male @entity35 . Damage to the airway epithelium was assayed by monitoring the ciliary beat frequency, the release of 51Cr, and histology. @entity274 at concentrations of 1.0 mM and above caused a very rapid decrease in ciliary beat frequency. After ten minutes' exposure to 1.0 mM, the ciliary beat frequency was 72 +/- 20 percent of control. Release of 51Cr was a less sensitive measure with significant release occurring after four hours of exposure to ciliotoxic concentrations of @entity274 . Histologic changes were not evident within the experimental time period. All toxic effects of @entity274 were completely blocked by catalase. This study shows that @entity274 causes a rapid decline in ciliary activity and suggests that oxidant-mediated ciliary dysfunction could play a role in the pathogenesis of @entity1030 . The ciliary beat frequency provides a sensitive, physiologically relevant parameter for the in vitro study of these diseases.
