Changes in NMDA receptor subunit gene expression in the XXXX brain following withdrawal from forced long-term @entity1068 intake.

@entity1 @entity4477 @entity410 @entity1024 @entity4319 @entity988 @entity4475 @entity4476 @entity4474 @entity35 @entity1068
Changes in mRNA levels of @entity1024 receptor (NR) subunits were studied in a @entity35 model of withdrawal from forced @entity1068 ingestion over a period of 8 days. In part, this model may reflect the epsilon-type of @entity1 @entity988 according to Jellinek (College University Press, New Haven; 1972). The epsilon-type is characterized by dipsomania over a period of several days, recurring every few months and often followed by @entity1068 -induced @entity410 . @entity410 may be modulated by an increased glutamatergic neurotransmission to excitatory or inhibitory neurons on the basis of a changed gene expression of NR subunits. This hypothesis promoted the present study. Film autoradiograms and emulsion-coated brain sections following labeling of cholinergic and GABAergic neuron populations were evaluated. @entity4474 ( @entity4474 ) expression, studied with a probe recognizing all @entity4474 transcripts, was unchanged after withdrawal from chronic @entity1068 treatment compared to control animals. Using probes specific for different splice segments of @entity4474 , however, we found that, in @entity1068 -treated @entity35 , the expression of @entity4474 -2 was decreased in all, and that of @entity4474 -4 in all but one, areas investigated (only single label experiments were performed with @entity4474 splice variants). Withdrawing @entity35 revealed a higher expression of @entity4476 ( @entity4476 ) mRNA in GABAergic neurons. No changes could be observed at the regional level. Conversely, @entity4319 mRNA was not substantially altered in cholinergic and GABAergic neurons, but showed a decrease over brain areas. For both, @entity4477 and @entity4475 , no @entity1068 -related changes of mRNA expression were observed. A link between such differential alterations in NR mRNA subunit expression and @entity1068 -induced @entity410 in withdrawing alcoholics of the epsilon-type seems possible.
