Effect of chondroitinase ABC on matrix metalloproteinases and inflammatory mediators produced by intervertebral disc of XXXX in vitro.

@entity1421 @entity2501 @entity95 @entity842 @entity2502
STUDY DESIGN: Lumbar intervertebral discs in @entity95 were cultured in the presence of chondroitinase ABC. The matrix metalloproteinases (MMPs) and inflammatory mediators produced in culture media were then analyzed. OBJECTIVES: To investigate the effect of chondroitinase ABC on MMPs and inflammatory mediators produced by intervertebral disc of @entity95 in vitro. SUMMARY OF BACKGROUND DATA: The chemonucleolytic effect of chondroitinase ABC is caused by the decrease in the chondroitin sulfate, hyaluronan, and protein content of the nucleus pulposus in @entity95 . The reason for the decreases in protein content remains unclear. METHODS: Anulus fibrosus and nucleus pulposus were cultured for 72 hours with or without chondroitinase ABC stimulated or not stimulated by interleukin-1 after preculture for 4 days. Subsequently, the MMPs (gelatinases @entity2501 , @entity2502 , and collagenase) and inflammatory mediators ( @entity1421 and @entity842 ) produced in the culture media were analyzed. RESULTS: In the anulus fibrosus chondroitinase ABC and interleukin-1 synergistically increased the collagenase activity, which was at a significantly higher level than the increment solely due to interleukin-1. In contrast, chondroitinase ABC counteracted the increase in @entity842 production by interleukin-1. In the nucleus pulposus the collagenase and @entity842 productions were not particularly affected by chondroitinase ABC and/or interleukin-1. In zymographic analysis @entity2501 was detected, but @entity2502 was only slightly detected in both tissues. There were no significant differences in both tissues for @entity2501 and @entity1421 following incubation with or without chondroitinase ABC, whether stimulated by interleukin-1 or not. CONCLUSIONS: The collagenase activity in the anulus fibrosus was increased by chondroitinase ABC with interleukin-1. This finding may support the hypothesis that some proteolytic activities are involved in the chemonucleolytic process by chondroitinase ABC treatment.
