Quantification of telomerase activity of regions unstained with XXXX solution that surround @entity957 .

@entity1 @entity2870 @entity1496 @entity5115 @entity5 @entity957 @entity189 @entity1141
The aim of this study was to analyze the @entity1496 -unstained region expanding around @entity957 ( @entity957 ) by quantification of telomerase activity. The @entity5115 often observed around @entity957 is considered to cause local recurrence or a second @entity5 . However these areas are hard to distinguish from normal mucosa. To clear the border of the expanding @entity5115 around @entity957 , we stained with 3% @entity1496 solution, and then decided the surgical margin. We measured quantification of telomerase activity in @entity5 , in @entity5115 , and also in normal epithelium. Thirty-three primary cases of oral @entity957 which have @entity1496 -unstained region around lesions were investigated. Fluorescense-based @entity2870 was applied to obtain quantification of telomerase activity. We obtained the following results: histological examination confirmed that every @entity1 's unstained region consisted of various degrees of @entity5115 . The quantified telomerase activities for @entity957 , @entity5115 and normal epithelium were 53.9, 39.6 and 2.7 U/microgP, respectively, and there was a significant difference between @entity189 and normal areas, and between @entity1141 and normal epithelium. Therefore, these findings suggest that the areas of @entity5115 unstained by @entity1496 consist of cells that are nearly cancerous and excessively proliferative, and that @entity5115 around @entity957 should be resected together with the @entity5 . Vital staining with @entity1496 is useful for identifying @entity5115 around @entity957 .
