Enzymatic activities of @entity291 peripheral blood leukocytes and milk polymorphonuclear neutrophils during intramammary XXXX caused by lipopolysaccharide.

@entity32 @entity70931 @entity1431 @entity1271 @entity291
Leukocytes are recruited from peripheral blood into milk as part of the inflammatory response to @entity1271 . However, excessive accumulation of inflammatory cells alters the quality of milk and the proteases produced by polymorphonuclear neutrophils (PMNs) and macrophages may lead to mammary tissue damage. To investigate PMN recruitment and the kinetics of their intracytoplasmic enzymes in @entity32 , we generated @entity1271 in six @entity291 by intramammary infusion of lipopolysaccharide (LPS). Clinical signs of acute @entity1271 were observed in all of the @entity291 , and normal status was resumed by 316 h. Intracytoplasmic elastase, collagenase, and cathepsin activities were measured within live cells by flow cytometry in peripheral blood leukocytes and milk PMNs before and during the inflammatory process (at 10 time points between 4 and 316 h). The proportion of immature PMNs was appreciated by @entity70931 surface labeling measured in flow cytometry. @entity1431 was observed in the peripheral blood 4 h postinfusion, concomitant to an increase in somatic cell counts in milk. @entity70931 (+) PMNs were preferentially recruited from the peripheral blood to milk. Enzymatic activities were detected in PMNs, lymphocytes, and monocytes at levels depending on the cell type, sample nature, and time of collection. Milk PMNs had lower enzymatic activities than peripheral blood PMNs. This study showed that milk PMNs recruited during LPS-induced experimental @entity1271 have an immature phenotype and significantly lower enzymatic activities than peripheral blood PMNs. This suggests that @entity70931 , an adhesion molecule, may be involved in the egress from blood to milk and that the enzymatic contents of PMNs are partly used during this process.
