The mode of action of XXXX , F2 alpha and prostacyclin on vesicourethral smooth muscle.

@entity1169 @entity4033 @entity1421 @entity1855 @entity1854 @entity95 @entity4527 @entity5521 @entity3897 @entity141 @entity11348
Interactions of @entity1421 ( @entity1421 ), F2 alpha (PGF2 alpha) and prostacyclin (PGI2) with @entity141 on the isometric contraction of @entity95 detrusor muscle strips were studied using two types of @entity141 antagonists of different mechanisms of action: @entity1855 and @entity1854 (NP). The effects of PGI2 on vesicourethral smooth muscle and their relationship with cholinergic, adrenergic receptors and nervous activity were also investigated. @entity1421 and F2 alpha (3 X 10(-8) to 3 X 10(-5) M) caused dose-dependent contraction of the strips. Pretreatment of the strips with @entity1855 (10(-7) to 10(-5)M) significantly inhibited PGs-induced contraction in a dose-dependent manner, whereas NP(10(-7) to 10(-5)M) failed to suppress the contraction. Relaxation of the preparations once contracted by @entity1421 and F2 alpha (3 X 10(-6)M) was induced completely by addition of @entity1855 (10(-5)M), and incompletely by NP(10(-5) to 10(-3)M). Washing of the strips with @entity141 -free solution containing 0.01 mM @entity4527 completely eliminated spontaneous activity and diminished basal tension, but replenishment of @entity141 (0.5 to 10 mM) to the medium caused dose-related contraction and spontaneous activity of the strips. Addition of @entity1421 and F2 alpha to the @entity141 -free medium enhanced @entity141 -induced contraction and spontaneous activity during @entity141 replenishment, which were significantly inhibited by pretreatment with @entity1855 (10(-7) to 10(-5)M) in a dose-dependent manner, but not affected by NP (10(-7) to 10(-5)M). In @entity141 -free medium containing 0.1 mM @entity4527 , @entity1421 and F2 alpha caused a slight degree of tension increase of the strips dose-dependently at the higher concentration exceeding 3 X 10(-6)M. PGI2 (10(-9) to 3 X 10(-4)M) caused dose-dependent contraction of the strips from the bladder body, base and the urethra. The contractile action of PGI2 was greatest on the bladder body, less on the base and minimal on the urethra. The effect of PGI2 was less potent than those of @entity1421 and F2 alpha. The PGI2-induced contraction was slow in onset, short lasting, and not affected by pretreatment with @entity5521 , @entity1169 , @entity3897 , @entity4033 , @entity11348 and tetrodotoxin. The interactions of PGI2 with @entity141 were similar to those of @entity1421 and F2 alpha.(ABSTRACT TRUNCATED AT 400 WORDS)
