Different effects of @entity535 -based and @entity413 -based dialysate from peritoneal dialysis XXXX on mesothelial cell ultrastructure and function.

@entity1 @entity650 @entity1522 @entity535 @entity1726 @entity98 @entity880 @entity413
BACKGROUND: Peritoneal dialysis fluid (PDF) containing @entity1522 has been introduced recently aiming to improve the nutritional status of @entity1726 @entity1 . @entity413 -based PDFs have been implicated in progressive functional and structural deterioration of the peritoneal membrane. Limited data are currently available regarding the effect of @entity535 -based PDF on the function and ultrastructure of @entity1 peritoneal mesothelial cells (HPMCs), which play a critical role in peritoneal membrane pathophysiology. METHODS: We investigated the effects of two commercially available PDFs, which utilized @entity413 (1.5% Dianeal) or @entity1522 (1.1% Nutrineal) as the osmotic agent, obtained from @entity1 after a 4 h dwell, on HPMC proliferation ( @entity98 assay and cell counting) and viability [ @entity880 dehydrogenase (LDH)release], @entity650 ( @entity650 ) secretion (commercial enzyme-linked immunosorbent assay) and ultrastructure (scanning and transmission electron microscopy). RESULTS: Exposure of HPMCs to 1.5% Dianeal reduced cell proliferation, total cellular protein synthesis, @entity650 secretion and cell attachment, but prolonged the cell doubling time on recovery, and increased LDH release (P<0.001, P<0.001, P<0.0001, P<0.0001, P<0.001 and P<0.001, respectively). The 1.1% Nutrineal reduced HPMC proliferation (P<0.001) and increased @entity650 secretion (P<0.0001), but did not affect cell attachment, LDH release, protein synthesis or cell doubling time. Ultrastructural studies of HPMCs exposed to Dianeal showed cell flattening, increased cell surface area, reduced microvilli, and intracellular organelles compatible with dysfunctional mitochondria. In contrast, the ultrastructural morphology of HPMCs was relatively preserved after incubation with Nutrineal. CONCLUSIONS: Our results showed that HPMC ultrastructure, viability and protein synthesis were better preserved with @entity535 -based PDF, compared with conventional dextrose-based PDF. The significance of @entity650 induction by Nutrineal remains to be elucidated.
