Gene expression profile in newborn XXXX lungs after two days of recovery of mechanical ventilation.

@entity1 @entity558 @entity26 @entity2107 @entity35 @entity44 @entity1210
BACKGROUND: Preterm @entity1 having @entity44 lungs often require respiratory support, potentially leading to @entity2107 ( @entity2107 ). Conventional @entity2107 rodent models based on mechanical ventilation (MV) present outcome measured at the end of ventilation period. A reversible intubation and ventilation model in newborn @entity35 recently allowed discovering that different sets of genes modified their expression related to time after MV. In a newborn @entity35 model, the expression profile 48h after MV was analyzed with gene arrays to detect potentially interesting candidates with an impact on @entity2107 development. METHODS: @entity35 pups were injected P4-5 with 2mg/kg @entity558 . One day later MV with 21% or 60% @entity26 was applied during 6 hours. Animals were sacrificed 48 hours after end of ventilation. Affymetrix gene arrays assessed the total gene expression profile in lung tissue. RESULTS: In fully treated animals ( @entity558 +MV+60%O2) vs controls, 271 genes changed expression significantly. All modified genes could be classified in six pathways: tissue remodeling/wound repair, immune system and inflammatory response, @entity1210 , vasodilatation, and oxidative stress. Major alterations were found in the MMP and complement system. CONCLUSION: MMPs and complement factors play a central role in several of the pathways identified and may represent interesting targets for @entity2107 -treatment/prevention.Pediatric Research (2015); doi:10.1038/pr.2015.155.
