Evaluation of the antigenicity of hydrolyzed XXXX 's milk protein formulas using the @entity19 basophil activation test.

@entity1 @entity19712 @entity44760 @entity6132 @entity19 @entity291 @entity2021
UNASSIGNED: Hypoallergenic @entity1 formulas are widely used for @entity1 with @entity291 's @entity6132 . The aim of this study was to assess the utility of the @entity19 basophil activation test (BAT) in the evaluation of residual antigenicity in these formulas. Whole blood samples derived from b-lactoglobulin- or casein-immunized @entity19 were incubated with one of the following formulas: conventional, partially hydrolyzed, or extensively hydrolyzed. Basophilic activation was analyzed by flow cytometry using an IgE-dependent activation marker @entity19712 and an IgG-dependent activation marker @entity44760 . @entity2021 was induced by i.v. injection of milk formula and results were compared. Conventional formula induced pronounced changes in @entity19712 and @entity44760 expression on basophils, whereas extensively hydrolyzed formulas did not elicit any changes in these markers. Similarly, challenge with conventional formula induced @entity2021 , whereas extensively hydrolyzed formulas did not induce @entity2021 . Although the partially hydrolyzed formula also induced basophilic activation and @entity2021 , the magnitude of these effects was smaller than that observed with the conventional formula. Compared to @entity19712 , the observed trend in @entity44760 expression resembled the results obtained from @entity2021 test more closely. These findings show that @entity19 BAT, in particular using @entity44760 , is highly useful for the evaluation of antigenicity of milk formulas.
