XXXX crystals stimulate gene expression in renal epithelial cells.

@entity1 @entity82 @entity5201 @entity2289 @entity3835 @entity517 @entity35635 @entity177 @entity1790 @entity5738 @entity10812 @entity5282 @entity2290 @entity12020
Primary or secondary @entity5201 is associated with @entity3835 @entity1790 , @entity82 and progressive @entity177 . Monolayer cultures of nontransformed monkey kidney epithelial cells ( @entity35635 line) and @entity3835 ( @entity10812 ) crystals were used as a model system to study cell responses to crystal interactions that might occur in the nephrons of @entity1 during periods of @entity5201 . To determine if @entity10812 crystals signal a change in gene expression, Northern blots were prepared from total renal cellular RNA after the cells were exposed to crystals. The immediate early genes @entity517 , @entity5738 , and @entity5282 were induced at one hour. At two to six hours stimulated expression of the genes encoding plasminogen activator inhibitor ( @entity2289 ) and platelet-derived growth factor (PDGF)-A chain was detected, but constitutive expression of @entity2290 ( @entity2290 ) was not altered. Expression of @entity12020 ( @entity12020 ) was induced at one hour and persisted up to 24 hours. The stimulation of gene expression by @entity10812 crystals was relatively crystal- and renal cell-type specific. Thus the interaction of kidney epithelial cells with @entity10812 crystals alters expression of genes that encode three classes of proteins: transcriptional activators, a regulator of extracellular matrix (ECM), and growth factors. Activation of @entity2289 gene expression without a change in @entity2290 favors accumulation of ECM proteins, as does increased expression of PDGF and @entity12020 which can also stimulate fibroblast proliferation in a paracrine manner. These results suggest that @entity10812 crystal-mediated stimulation of specific genes in renal tubular cells may contribute to the development of @entity82 in hyperoxaluric states.
