The relationship between hippocampal @entity934 release and cholinergic convulsant sensitivity in withdrawal @entity410 -prone and withdrawal XXXX -resistant selected @entity19 lines.

@entity410 @entity4024 @entity19 @entity10406 @entity1239 @entity4778 @entity400 @entity972 @entity3451 @entity285 @entity1339 @entity1068 @entity5402 @entity934
BACKGROUND: The septo-hippocampal cholinergic pathway has been implicated in epileptogenesis, and genetic factors influence the response to cholinergic agents, but limited data are available on cholinergic involvement in @entity285 withdrawal severity. Thus, the relationship between cholinergic activity and responsiveness and @entity285 withdrawal was investigated in a genetic animal model of @entity1068 withdrawal severity. METHODS: Cholinergic convulsant sensitivity was examined in @entity285 - @entity4024  ve Withdrawal @entity410 -Prone (WSP) and-Resistant (WSR) @entity19 . Animals were administered @entity1239 , @entity5402 , or @entity1339 via timed tail vein infusion, and the latencies to onset of @entity400 and clonus were recorded and converted to threshold dose. We also used microdialysis to measure basal and @entity972 -stimulated @entity934 ( @entity3451 ) release in the @entity4778 region of the hippocampus. @entity972 was applied by reverse dialysis twice, separated by 75 min. Hippocampal @entity3451 also was measured during testing for handling-induced @entity410 . RESULTS: Sensitivity to several @entity410 endpoints induced by @entity1239 , @entity5402 , and @entity1339 were significantly greater in WSR versus WSP @entity19 . In microdialysis experiments, the lines did not differ in basal release of @entity3451 , and 50 mM @entity10406 increased @entity3451 output in both lines of @entity19 . However, the increase in release of @entity3451 produced by the first application of @entity10406 was 2-fold higher in WSP versus WSR @entity19 . When hippocampal @entity3451 was measured during testing for handling-induced @entity410 , extracellular @entity3451 was significantly elevated (192%) in WSP @entity19 , but was nonsignificantly elevated (59%) in WSR @entity19 . CONCLUSIONS: These results suggest that differences in cholinergic activity and postsynaptic sensitivity to cholinergic @entity410 may be associated with @entity1068 withdrawal severity and implicate cholinergic mechanisms in @entity285 withdrawal. Specifically, WSP @entity19 may have lower sensitivity to cholinergic @entity410 compared with WSR because of postsynaptic receptor desensitization brought on by higher activity of cholinergic neurons.
