Potential involvement of BMP receptor type IB activation in a synergistic effect of chondrogenic promotion between rhTGFbeta3 and rhGDF5 or rhBMP7 in XXXX mesenchymal stem cells.

@entity1 @entity7382 @entity3823 @entity15703 @entity1842 @entity30713 @entity19126 @entity12208 @entity17507
Chondrogenic promotion by rhGDF5 with or without rhTGFbeta3 was studied in pellet culture of @entity1 mesenchymal stem cells (HMSCs). A synergy between rhGDF5 and rhTGFbeta3 was observed in promoting chondrogenesis. rhBMP2, rhBMP6, rhBMP7 and rhTGFbeta1 were further tested and showed the same effect. To explore the mechanism, the expression of TGFbetatype I and II receptors, @entity7382 , @entity15703 , @entity1842 , @entity30713 , TGFbetaRII, @entity3823 , @entity19126 was studied. @entity30713 showed increase by the rhTGFbeta1 or rhTGFbeta3 treatment. @entity30713 protein expression also showed increase by rhTGFbeta3. rhTGFbeta1/rhTGFbeta3 induced @entity30713 up-regulation was inhibited by @entity17507 , a TGFbeta type I receptor inhibitor. Chondrogenesis by rhTGFbetal/rhTGFbeta3 or the combination between rhTGFbetal/rhTGFbeta3 and rhGDF5 also was diminished by @entity17507 . @entity12208 /8 phosphorylation in nascent @entity1 mesenchymal stem cells (HMSCs) was stimulated weakly by rhGDF5 but strongly by rhBMP7. The rhGDF5 stimulated @entity12208 /8 phosphorylation was enhanced by rhTGFbetal/rhTGFbeta3 but inhibited by @entity17507 . The rhBMP7 stimulated @entity12208 /8 phosphorylation did not show influence by rhTGFbeta3 and @entity17507 . Our results indicated the potential involvement of @entity30713 activation by rhTGFbetas in the synergy between rhTGFbetas and rhBMPs.
