Establishment of XXXX -Based Luciferase Viability Assay in 96-Well Plate for @entity3177 congolense.

@entity4409 @entity3177 @entity7341 @entity12696 @entity855
@entity4409 ( @entity4409 ), caused by @entity3177 congolense, is widespread throughout sub-Saharan Africa. There are significant concerns related to the current drugs available for the treatment of @entity4409 due to their limited effectiveness across species and their adverse effects. Moreover, drug resistant trypanosomes have recently been reported in the field. High throughput screening (HTS) of large chemical compound library collections is a promising approach for identifying novel drug candidates. While HTS for Trypanozoon trypanosomes, @entity3177 sspp. and T. evansi is well established, no assays have been developed for T. congolense. In the present study, the authors developed an @entity855 -based luciferase viability assay for T. congolense in a 96-well plate format. The calculated 50% inhibitory concentration (IC50) values for @entity7341 and @entity12696 were 10-100 times higher in T. congolense than in @entity3177 . This result suggests that the transporters for the two tested compounds differ between T. congolense and @entity3177 . This assay could further be applied to screen novel chemical compounds for the treatment of @entity4409 caused by T. congolense.
