atBRX1-1 and atBRX1-2 are involved in an alternative rRNA processing pathway in XXXX .

@entity1 @entity958 @entity71356 @entity69086 @entity375
Ribosome biogenesis is an essential process in all organisms. In eukaryotes, multiple ribosome biogenesis factors (RBFs) act in the processing of ribosomal (r)RNAs, assembly of ribosomal subunits and their export to the cytoplasm. We characterized two genes in @entity958 coding for orthologs of @entity375 @entity69086 , a protein involved in maturation of the large ribosomal subunit. Both atBRX1 proteins, encoded by AT3G15460 and AT1G52930, respectively, are mainly localized in the nucleolus and are ubiquitously expressed throughout plant development and in various tissues. Mutant plant lines for both factors show a delay in development and pointed leaves can be observed in the @entity69086 -2 mutant, implying a link between ribosome biogenesis and plant development. In addition, the pre-rRNA processing is affected in both mutants. Analysis of the pre-rRNA intermediates revealed that early processing steps can occur either in the 5' external transcribed spacer (ETS) or internal transcribed spacer 1 (ITS1). Interestingly, we also find that in @entity71356 mutants, early processing events can be bypassed and removal of the 5' ETS is initiated by cleavage at the P' processing site. While the pathways of pre-rRNA processing are comparable to those of @entity375 and @entity1 cells, the balance between the two processing pathways is different in plants. Furthermore, plant-specific steps such as an additional processing site in the 5' ETS, likely post-transcriptional processing of the early cleavage sites and accumulation of a 5' extended 5.8S rRNA not observed in other eukaryotes can be detected.
