Intracellular free XXXX in the neutrophils of maintenance haemodialysis @entity1 .

@entity1 @entity137 @entity5038 @entity1307 @entity177 @entity460 @entity141 @entity461
@entity177 has on occasion been referred to as a state of @entity460 @entity137 . The aim of this study was to investigate the status of intracellular free @entity141 in the neutrophils of @entity177 @entity1 on maintenance haemodialysis treatment. Factors previously suggested to influence intracellular free @entity141 were investigated including @entity461 levels, oxidative stress and recombinant @entity1 @entity1307 administration. The study involved 14 @entity177 @entity1 on the haemodialysis programme of the Pretoria Academic hospital. Intracellular free @entity141 and transmembrane @entity141 fluxes were investigated by fluorescence spectrophotometry. Increases above control values were found in intracellular free @entity141 (P-value 0.0242) and in the transmembrane @entity141 flux upon @entity5038 stimulation (P-value 0.0002). The results showed significant differences in intracellular free @entity141 between @entity1 on rHuEPO and @entity1 not on rHuEPO. The apparently rHuEPO-induced increase in intracellular free @entity141 persisted in the presence of @entity460 channel blockers. No overt indications of oxidative stress could be detected by the antioxidant vitamin levels. It is concluded that factors other than those associated with uraemia, such as rHuEPO administration, might contribute to the often reported increase in intracellular free @entity141 in these @entity1 . Further studies to investigate the relationship between intracellular free @entity141 , rHuEPO and @entity460 channel blockers are suggested.
