NanoCluster XXXX Formulations Provide a Potential Engineered Drug Particle Approach to Generate Effective Dry Powder Aerosols.

@entity1 @entity167 @entity10998 @entity364 @entity400 @entity5929 @entity3 @entity2059
BACKGROUND: @entity2059 (ITZ), a @entity10998 antifungal agent, is a poorly water-soluble drug that is orally administered for treatment of @entity364 such as @entity5929 ( @entity5929 ) and @entity3 ( @entity3 ). @entity5929 is relatively well controlled but @entity3 can be fatal, especially in immunosuppressed @entity1 . Aerosolized ITZ delivered to the lung may provide a local treatment and prophylaxis against @entity3 at the primary site of infection in the lungs. Variations of the percent @entity400 ( @entity400 ), the percent emitted dose, and the physical properties of the aerosol (e.g., crystallinity) can confound consistent delivery. METHODS: ITZ NanoClusters were formulated via milling (top-down process) or precipitation (bottom-up process) without using any excipients. @entity2059 formulations (ITZ) were prepared by milling 1 gram of micronized @entity2059 in 300   mL of fluid. The suspension was collected at 0.5, 1, and 2 hours milling time. Milled ITZ was compared to ITZ prepared by anti-solvent precipitation and to the stock micronized @entity2059 . The aerosolization performance of ITZ formulations was determined using an Andersen Cascade Impactor (ACI). RESULTS: The physicochemical properties and aerosol performance of different ITZ NanoClusters suggested an optimized wet milling was the preferred process compared to precipitation. ITZ NanoClusters prepared by wet milling showed better aerosol performance compared to micronized ITZ as received and ITZ NanoClusters prepared by precipitation. ITZ NanoClusters prepared by precipitation methods also showed an amorphous state, while ITZ milled in 10% @entity167 maintained the crystalline character of ITZ throughout a 2 hour milling time. CONCLUSIONS: The aerosol performance of milled ITZ NanoClusters was dramatically improved compared to micronized ITZ as received due to the difference of drug particle structures. ITZ NanoCluster formulations represent a potential engineered drug particle approach for inhalation therapy, providing effective aerosol properties and stability due to the crystalline state of the drug powders.
