Central pathways in the pons and midbrain involved in cardiac sympathoexcitatory reflexes in XXXX .

@entity101 @entity19222 @entity591 @entity158 @entity1812 @entity468
Activation of cardiac sympathetic afferents elicits @entity158 and excitatory cardiovascular reflexes including acute @entity101 and @entity591 . Our previous studies have shown that specific regions in the medulla, such as the nucleus of solitary tract and ventrolateral medulla, are involved in central regulation of cardiac sympathoexcitatory reflexes. However, the contributions of supramedullary nuclei to these reflexes have not been characterized. In the present study, we located activated neurons in the pons and midbrain induced by inputs from cardiac sympathetic afferents by detecting their @entity19222 immunoreactivity. In anesthetized @entity468 with bilateral carotid denervation and cervical vagotomy, epicardial application of @entity1812 (1-10 microg, in 0.1 ml; n=7) was performed on the anterior surface of the left ventricle six times, every 20 min. Repetitive application of @entity1812 caused consistent excitatory cardiovascular reflexes characterized by increases in blood pressure and heart rate. No responses were evoked by the vehicle for @entity1812 (0.9% saline, n=7). Compared to control @entity468 , @entity19222 immunoreactive cells were significantly increased (P<0.05) in the rostral pons, caudal and intermediate midbrain in the @entity1812 -treated @entity468 . The specific areas activated include the parabrachial nucleus, K  lliker-Fuse nucleus, locus coeruleus, dorsal nucleus of raphe, and dorsal, lateral and ventrolateral periaqueductal gray. From these results we suggest that cardiovascular-related regions in the pons and midbrain form part of a long loop in central integration of cardiac sympathoexcitatory reflexes.
