XXXX 2B1 mediates oxidant injury in puromycin-induced @entity1985 .

@entity4444 @entity1447 @entity8 @entity644 @entity274 @entity35 @entity26 @entity4921 @entity20391 @entity7540 @entity273 @entity8424 @entity1985 @entity68 @entity1446
BACKGROUND: Reactive @entity26 metabolites (ROM) are important mediators of @entity8424 ( @entity7540 ) induced minimal @entity1985 ( @entity1985 ) in @entity35 . We have recently shown that @entity1447 ( @entity1447 ) is a significant source of catalytic @entity68 in this model of @entity8 . The current study was designed to identify the @entity1447 isozyme(s) in the @entity35 glomeruli and explore the role of the specific isozyme(s) in @entity7540 -induced minimal change @entity1985 . METHODS: @entity1985 was induced in @entity35 by a single intravenous injection of @entity7540 . Animals were sacrificed at different time points for variety of biochemical assays including Western blot, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Ultrastructural histochemistry was utilized to study @entity273 ( @entity274 ) generation in the kidney. RESULTS: Several @entity1447 isozymes were tested and @entity4921 was localized exclusively in the @entity35 glomeruli but not in the tubules. Treatment with @entity7540 resulted in the generation of @entity274 in the glomerular basement membrane with significant loss of @entity4921 content accompanied by a marked increase in the catalytic @entity68 . @entity4921 inhibitors @entity4444 and @entity20391 significantly reduced @entity274 generation, and prevented the loss of @entity4921 content and the increase in the catalytic @entity68 . @entity4921 inhibitors also provided significant protection against @entity7540 induced @entity644 . The induction of @entity1446 oxygenase and ferritin also was observed in the glomeruli in @entity7540 -treated @entity35 . Both @entity4444 and @entity20391 reduced the up-regulation of these proteins. CONCLUSION: Our data indicate that @entity4921 plays an important role in @entity7540 induced @entity1985 by serving as a site for ROM generation and a significant source of catalytic @entity68 .
