For t 2 DNA Vaccine Prevents Forcipomyia taiwana ( XXXX ) Allergy in a @entity19 Model.

@entity32 @entity19 @entity566 @entity14197 @entity56 @entity58 @entity1312
BACKGROUND: Forcipomyia taiwana ( @entity14197 ) is the most prevalent allergenic biting insect in Taiwan and 60% of the exposed subjects develop @entity56 . Subjects with insect @entity566 frequently limit their outdoor activities to avoid the annoyingly intense @entity56 , leading to significant worsening of their quality of life. Allergen specific immunotherapy is the only known therapy that provides long-term host immune tolerance to the allergen, but is time-consuming and cumbersome. This study tested if the For t 2 DNA vaccine can prevent @entity56 in For t 2 sensitized @entity19 . MATERIALS AND METHODS: Two consecutive shots of For t 2 DNA vaccine were given to @entity19 with a 7-day interval before sensitization with recombinant For t 2 proteins, using the two-step sensitization protocol reported previously. RESULTS: The For t 2 DNA vaccine at 50  g prevented the production of For t 2-specific IgE (p<0.05), as well as midge-allergen challenge induced scratch bouts, midge-allergen induced @entity1312 and @entity58 production from splenocytes, and inflammatory cell infiltrations in the lesions 48 hours after intra-dermal challenge. CONCLUSIONS: This study is the first to demonstrate that DNA vaccine encoding midge allergen is effective in preventing @entity32 induced by @entity14197 . Immunotherapy using For t 2 DNA vaccine can protect @entity19 from being sensitized by midge allergen and may be a promising treatment for @entity14197 @entity566 in the future. This article is protected by copyright. All rights reserved.
