[Regulatory role of serum @entity14908 in invasiveness and @entity3 of XXXX ].

@entity1 @entity650 @entity5 @entity1126 @entity201 @entity4435 @entity3 @entity14908
OBJECTIVE: To investigate the expression profile of serum micro (mi)RNAs in @entity201 ( @entity4435 ) and investigate the regulatory contribution of miRNAs to the invasive and @entity3 . METHODS: Microarray analysis was carried out using serum samples collected from 30 @entity1 with @entity4435 , @entity1126 tissues and the corresponding normal tissues collected from 10 patients, and serum samples from 50 healthy volunteers. The miRNAs identified as dysregulated in @entity4435 were verified by RT-PCR. Focused analysis on @entity14908 was carried out using the @entity1 @entity4435 cell lines HCCC-9810 and RBE to investigate the role of this miRNA in @entity650 expression (using @entity650 induction), cell growth, invasiveness and @entity3 (using @entity14908 mimic transfection). The one-way ANOVA test was used for statistical analysis. RESULTS: Forty-three miRNAs were dysregulated in @entity4435 (vs. @entity4435 , P<0.01), of which 22 were upregulated and 21 were downregulated. RT-PCR data showed that the @entity14908 was significantly upregulated in serum as well as in @entity5 tissue from @entity4435 @entity1 . Induction of HCCC-9810 and RBE cells with @entity650 showed a time-dependent upregulation of @entity14908 . Furthermore, the HCCC-9810 and RBE cells transfected with @entity14908 mimic showed enhanced cell growth, invasiveness and migratory ability. CONCLUSION: @entity650 may promote the invasive and metastatic properties of @entity4435 through upregulated @entity14908 . Studies of the differentially expressed serum miRNAs in @entity4435 may help to further elucidate the pathogenic processes of this disease and aid in the development of a novel and effective therapeutic strategy.
