Establishment of a @entity2 cell line (Y-ML-1B) producing XXXX .

@entity1 @entity6076 @entity1148 @entity1261 @entity986 @entity5 @entity19 @entity984 @entity2 @entity7465 @entity5962 @entity2526 @entity876 @entity15893 @entity8497 @entity687 @entity2841 @entity491 @entity995
We established a new @entity2 cell line, designated Y-ML-1B, from a @entity2 of a 70-year-old Japanese @entity1 with @entity491 and @entity1148 . Before surgical resection, the white blood cell and platelet counts were elevated to 34,400/mm3 and 668,000/mm3, respectively, and the @entity1261 ( @entity1261 ) level in the serum was increased at 141 pg/mL. The @entity5 showed an undifferentiated morphology with large cells and induced extensive thickening of the pleura in the right hemithorax. The Y-ML-1B cells grow as a monolayer, with a doubling time of 19 hours, and are tumorigenic in @entity19 , which showed a morphology similar to the @entity5 in xenografts. Analysis of the supernatant of cell culture medium of Y-ML-1B showed elevated levels of @entity1261 and other cytokines such as interleukin (IL)-6, @entity687 , and @entity2841 ( @entity2841 ), consistent with the high levels detected in the patient's serum. Cytogenetic analysis revealed aneuploidy of greater than 56 in metaphases with many @entity2526 . Mutation analysis of the @entity5 suppressor genes showed that Y-ML-1B is inactivated in @entity876 and @entity15893 , but not in @entity986 (ARF), @entity986 ( @entity986 ), or @entity995 . Neither activating mutations of @entity984 or @entity8497 nor amplification of MYC or @entity7465 were detected. Y-ML-1B expressed @entity6076 but not @entity5962 . This newly established cell line might serve as a useful model for studying the molecular pathogenesis for large cell cancers of the lung which express high levels of cytokines.
