The XXXX Mediator Complex Subunit16 Is a Key Component of Basal Resistance Against the Necrotrophic Fungal Pathogen @entity19868 .

@entity7214 @entity958 @entity46699 @entity19868 @entity18614 @entity45328 @entity49009 @entity375 @entity1786
UNASSIGNED: Although @entity19868 (Lib.) de Bary is a devastating necrotrophic fungal plant pathogen in agriculture, the virulence mechanisms utilized by @entity19868 and the host defense mechanisms against this pathogen have not been fully understood. Here, we report that the @entity958 Mediator complex subunit @entity49009 is a key component of basal resistance against @entity19868 . Mutants of @entity49009 are markedly more susceptible to @entity19868 than mutants of thirteen other Mediator subunits, and @entity49009 has a much stronger effect on @entity19868 -induced transcriptome changes compared with med8, a mutation not altering susceptibility to @entity19868 . Interestingly, @entity49009 is also more susceptible to @entity19868 than @entity46699 -insensitive1-1 (coi1-1), which is the most susceptible mutant so far reported. Although the @entity7214 (JA)/ @entity1786 (ET) defense pathway marker gene PLANT DEFENSIN1.2 ( @entity18614 ) cannot be induced in either @entity49009 or coi1-1, basal transcript levels of @entity18614 in @entity49009 are significantly lower than in coi1-1. Furthermore, ET-induced suppression of JA-activated wound responses is compromised in @entity49009 , suggesting a role for @entity49009 in JA-ET crosstalk. Additionally, @entity49009 is required for recruitment of RNA polymerase II to @entity18614 and OCTADECANOID-RESPONSIVE @entity958 AP2/ERF59 (ORA59), two target genes of both JA/ET-mediated and the transcription factor @entity45328 -activated defense pathways. Finally, @entity49009 is physically associated with @entity45328 in @entity375 and in planta, and @entity45328 -activated transcription of @entity18614 and ORA59 as well as resistance to @entity19868 depends on @entity49009 . Taken together, these results indicate that @entity49009 regulates resistance to @entity19868 by governing both JA/ET-mediated and @entity45328 -activated defense signaling in @entity958 .
