Differential chemokine responses and homing patterns of XXXX TCR alpha beta NKT cell subsets.

@entity31229 @entity12426 @entity13386 @entity14013 @entity19 @entity9843 @entity51934 @entity20782 @entity71544 @entity73013 @entity1961 @entity11386 @entity19149 @entity33686 @entity13385 @entity6232
NKT cells play important roles in the regulation of diverse immune responses. Therefore, chemokine receptor expression and chemotactic responses of @entity19 TCRalphabeta NKT cells were examined to define their homing potential. Most NKT cells stained for the chemokine receptor @entity14013 , while >90% of @entity33686 i-positive and approximately 50% of @entity33686 i-negative NKT cells expressed @entity71544 via an enhanced green fluorescent protein reporter construct. @entity9843 expression was higher on @entity33686 i-negative than @entity33686 i-positive NKT cells. In spleen only, subsets of @entity33686 i-positive and -negative NKT cells also expressed @entity51934 . NKT cell subsets migrated in response to ligands for the inflammatory chemokine receptors @entity14013 (monokine induced by @entity12426 ) and @entity71544 ( @entity31229 ), and regulatory chemokine receptors @entity13386 ( @entity13385 ( @entity13385 )/CC ligand (CCL)21), @entity9843 (stromal cell-derived factor-1/ @entity19149 ), and @entity51934 (B cell-attracting chemokine-1/ @entity20782 ); but not to ligands for other chemokine receptors. Two NKT cell subsets migrated in response to the lymphoid homing chemokine @entity13385 /CCL21: @entity1961 (-) Valpha14i-negative NKT cells that were @entity6232 (high) and enriched for expression of @entity73013 (consistent with the phenotype of most NKT cells found in peripheral lymph nodes); and immature @entity33686 i-positive cells lacking @entity11386 and @entity6232 . Mature @entity11386 (+) Valpha14i-positive NKT cells did not migrate to @entity13385 /CCL21. @entity20782 / @entity20782 , which mediates homing to B cell zones, elicited migration of @entity33686 i-positive and -negative NKT cells in the spleen. These cells were primarily @entity1961 (+) or @entity1961 (-)CD8(-) and were enriched for Ly49C/I, but not @entity73013 . Low levels of chemotaxis to @entity31229 were only detected in @entity33686 i-positive NKT cell subsets. Our results identify subsets of NKT cells with distinct homing and localization patterns, suggesting that these populations play specialized roles in immunological processes in vivo.
