Immunoscintigraphy of @entity1 XXXX xenografts using a radiolabelled monoclonal antibody to human pregnancy serum-derived immune complexes.

@entity1 @entity43 @entity19 @entity5
The in vivo imaging of xenografted @entity1 @entity43 in @entity19 with a specific and control radiolabelled monoclonal antibody (MoAb) is described. The specific MoAb was previously raised by immunizing @entity19 with immune complexes derived from late human pregnancy serum. In the first group of @entity19 the specific MoAb 131I-5E3 F(ab')2 was injected, while a second group received equivalent amounts of a control MoAb 131I-UJ13A F(ab')2. The @entity19 were imaged at various times up to a maximum of 2 weeks using a gamma camera, and the @entity5 to @entity5 (T/NT) ratio was recorded for each group. The T/NT ratio rose to 2.02 in the specific group, while the corresponding ratio in the control group was 0.70. In addition, the count rate in the @entity5 and @entity5 regions was determined on each imaging occasion. Biological half-lives of the divalent fragments of 5E3 and UJ13A in the @entity5 were 7.53 days and 0.62 days, respectively. Following sacrifice, the @entity5 were excised and counted relative to the rest of the animal, and the T/NT ratio was calculated. In vitro results were in direct agreement with those recorded in vivo using the gamma camera. From the results it would appear that the divalent fragment of 5E3, which has been raised to immune complexes derived from late human pregnancy serum, is specific for @entity1 @entity43 xenografts in the nude @entity19 model.
