Cryptosporidiosis in two @entity7858 ( XXXX ) holdings in the South-West of England.

@entity1 @entity7858 @entity294 @entity2432
Cryptosporidiosis was investigated on two @entity7858 ( @entity7858 ) holdings in the South-West of England. Diagnosis was initially confirmed in a cria with @entity294 from each holding. Cohort faeces samples were subsequently collected and examined for presence of @entity2432 oocysts by immunofluorescence microscopy. On the first holding, 30 samples (24 adults, 6 crias) were tested, and oocysts were detected in three of the cria samples but in none of the adults. On the second holding, 14 floor faeces samples representing apparently healthy crias and one faeces sample from a cria with @entity294 were collected. Oocysts were detected in four of the "healthy" faeces samples and the sample of diarrhoeic faeces. All isolates were confirmed as @entity2432 using polymerase chain reaction restriction fragment length polymorphism of the @entity2432 oocyst wall protein (COWP) and ssu rRNA genes. Sequence analysis of a 741bp region of ssu rDNA was carried out on nine of these and revealed high sequence homology with previously reported @entity2432 isolates. This investigation highlights the possibility of @entity7858 crias subclinically shedding oocysts, which has implications for epidemiology and transmission in animals as well as raising zoonotic concerns for @entity1 contacts. Gene sequencing of UK isolates from South American camelids is also described for the first time.
