Differential temporal expression of matrix metalloproteinases after XXXX : relationship to revascularization and wound healing.

@entity32 @entity131 @entity66 @entity19 @entity5780 @entity1235 @entity43314 @entity1566 @entity130
OBJECT: Matrix metalloproteinases (MMPs), particularly @entity1235 / @entity1235 , promote early @entity32 and barrier disruption after @entity1566 ( @entity1566 ). Early blockade of MMPs after injury provides neuroprotection and improves motor outcome. There is recent evidence, however, that @entity1235 and @entity5780 / @entity5780 participate in later wound healing in the injured cord. The authors therefore examined the activity of these gelatinases during revascularization and @entity66 in the contused @entity19 spinal cord. METHODS: Gelatinase activity was evaluated using gelatin zymography 24 hours after a mild, moderate, or severe @entity130 . The active form of @entity5780 was not detected, whereas @entity1235 activity was evident in all @entity1566 groups and rose with increasing injury severity. The temporal expression of gelatinases was then examined using gelatin zymography after a moderate @entity1566 . The active form of @entity1235 was most prominent at 1 day, extended through the early period of revascularization, and returned to control by 14 days. The active form of @entity5780 appeared at 7 days postinjury and remained elevated compared with that documented in sham-treated @entity19 for at least 21 days. Increased @entity5780 activity coincided with both revascularization and @entity66 . Using in situ zymography, gelatinolytic activity was detected in the meninges, vascular elements, glia, and macrophage-like cells in the injured cord. Results of immunolabeling confirmed the presence of gelatinase in vessels during revascularization and in reactive astrocytes associated with @entity66 . CONCLUSIONS: These findings suggest that although @entity43314 exhibit overlapping expression during revascularization, the former is associated with @entity131 responses and the latter with formation of a @entity66 .
