In vivo and in vitro studies with an atypical, rhinotropic isolate of XXXX .

@entity7970 @entity19 @entity1066 @entity1327 @entity9592
An atypical isolate of @entity1327 was investigated because of its consistent and reproducible production of gross nasal pathology following i.v. injection in Swiss albino @entity19 . Dose response to graded concentrations ranging from 1 X 10(2)-1 X 10(7) cells/ @entity19 yielded an LD50 of 1.4 X 10(3) cells/ @entity19 for the atypical rhinotropic strain H140 which was significantly less virulent (p less than 0.01) than our reference strain of @entity1327 . There was no significant difference in mortality following the injection of in vitro vs. in vivo passed inoculum. As early as two weeks after inoculation, this strain produced gross nasal enlargement to approximately 2-3 X normal dimensions with granulomatous and @entity1066 . The LD60 resulted in the greatest percentage of nasal involvement (85%). @entity1327 was demonstrated by culture and histopathology in the noses, brains, lungs, livers and kidneys. A temperature selection was indicated by findings of a lower temperature minimum for subcultures isolated from the noses relative to those isolated from the brain, and by the fact that the most densely populated organs following intraperitoneal injection were the testes. This route of inoculation resulted in cutaneous nasal involvement in a manner analogous to that following i.v. injection. The atypical isolate was unable to assimilate @entity7970 or @entity9592 but otherwise was entirely consistent with identification as @entity1327 and produced characteristic CNS and general organ system disease in addition to the rhinotropic cutaneous manifestations. The model characterized here in normal @entity19 may be of value in studies of fungal dermotropism.
