Effect of sera from male type I (insulin-dependent) diabetics on XXXX aortic smooth muscle cells in culture.

@entity1 @entity6 @entity1674 @entity165 @entity4023
The effects of sera from male type I (insulin-dependent) diabetics in variable metabolic control were studied on the proliferation and the synthesis of hyaluronic acid, collagen and non-collagenous proteins in cultured @entity1 aortic smooth muscle cells (HSMCs). Sera from diabetics in poor metabolic control had a decreased growth-promoting effect on HSMCs as compared with that of sera from non- @entity6 healthy male controls. The synthesis of hyaluronic acid in HSMCs was increased in the presence of type I @entity6 sera, irrespective of the metabolic control of serum donors. The synthesis of collagen by HSMCs was stimulated only by the @entity6 sera which were derived from diabetics in poor metabolic control. The synthesis of non-collagenous proteins in HSMCs was not affected by type I @entity6 sera. The stimulatory effect of @entity6 sera on hyaluronic acid synthesis by HSMCs correlated inversely with plasma HDL @entity165 of serum donors, whereas the effect of @entity6 sera on DNA and collagen syntheses in HSMCs showed an inverse correlation with plasma @entity1674 of serum donors. Plasma insulin-like growth factor-I did not correlate with the above functions of HSMCs. Addition of insulin and growth hormone directly into HSMC cultures had no effect on the proliferation or synthesis of connective tissue macromolecules of HSMCs. The results suggest that type I @entity6 sera contain factors other than insulin, growth hormone or insulin-like growth factor-I that affect the proliferation and synthesis of @entity4023 and collagen of HSMCs in culture.(ABSTRACT TRUNCATED AT 250 WORDS)
