Chemopreventive action of @entity619 and XXXX in oxidative stressed cells.

@entity1 @entity32 @entity5 @entity619 @entity1881 @entity273
INTRODUCTION: Recent research indicates a close connection of @entity32 and @entity5 as presumed by Virchow in 1893. The growing understanding of cellular signalling and regulatory pathways reveals multiple links between @entity32 and @entity5 . This study was designed to evaluate the influence of the anti-inflammatory drug @entity619 and the antioxidant @entity1881 on oxidative induced DNA damage, a major factor in the development of @entity5 . MATERIAL AND METHODS: Miniorgan cultures (MOC) of fresh biopsied @entity1 nasal mucosa were used to keep cells in their microenvironment and thus to mimic in vivo conditions. MOC were pretreated with @entity619 and @entity1881 in different concentrations on 1 or on 5 days before oxidative DNA damage was introduced by @entity273 . The effect of these substances on DNA damage was evaluated using the alkaline single cell microgel electrophoresis (Comet Assay). RESULTS: @entity619 induced slight, but considerable DNA fragmentation by itself. It effectively protected cells from @entity273 induced DNA damage, leading to a maximum decrease of about 45% when preincubated on 5 days at 20 microM. @entity1881 most effectively reduced oxidative DNA fragmentation by about 38% when MOC were pretreated 5 days at 20 microM. DISCUSSION: Our experimental data clearly shows the DNA protective action of @entity619 and @entity1881 with regard to oxidatively induced DNA damage, a major pathogenetic factor that @entity32 and @entity5 have in common.
