A monoclonal antibody (IN-1) which neutralizes neurite growth inhibitory proteins in the XXXX CNS recognizes antigens localized in CNS myelin.

@entity8381 @entity17 @entity8382 @entity30203 @entity35 @entity62 @entity1068 @entity1543
In previous studies two neurite growth inhibiting protein fractions of 35 and 250 kDa were identified in myelin preparations of the @entity35 CNS. These activities were not found in the myelin of PNS. A monoclonal antibody (mAb IN-1) was raised against the 250 kDa protein fraction and selected for its ability to neutralize the inhibitory effect of CNS myelin and of both isolated protein fractions. IN-1 has been shown both in vitro and in vivo to neutralize the inhibitory effect of differentiated @entity62 . In the present study, the antigens of IN-1 were localized by immunohistochemistry on cryostat sections of the adult @entity35 nervous system. The staining pattern of IN-1 was compared to that of mAbs specific for proteins found in CNS and PNS myelin. These proteins include @entity8382 , @entity8381 , and @entity30203 . IN-1 stained white matter and myelinated fibre tracts in the CNS on sections of fresh frozen tissue fixed with 95% @entity1068 : 5% @entity1543 (Clark's solution). @entity17 and spinal roots remained unstained. The staining pattern of IN-1 corresponded most closely to that of a mAb against @entity8381 , a protein which occurs exclusively in CNS myelin and on differentiated oligodendrocytes.
