Molecular diagnosis of @entity1330 in cerebrospinal fluid: comparison of primer sets for @entity1327 and XXXX species complex.

@entity1 @entity18204 @entity1330 @entity1327 @entity16 @entity14851 @entity791
AIM: This study evaluated the use of polymerase chain reaction for @entity1330 diagnosis in clinical samples. MATERIALS AND METHODS: The sensitivity and specificity of the methodology were evaluated using eight @entity1327 / @entity14851 species complex reference strains and 165 cerebrospinal fluid samples from @entity1 with @entity16 divided into two groups: 96 @entity1 with @entity1330 and @entity791 ; and 69 @entity1 with other @entity16 ( @entity18204 / @entity791 ). Two primer sets were tested (CN4-CN5 and the multiplex CNa70S-CNa70A/CNb49S-CNb-49A that amplify a specific product for @entity1327 and another for @entity14851 ). RESULTS: CN4-CN5 primer set was positive in all @entity1327 standard strains and in 94.8% in DNA samples from @entity1330 and @entity791 group. With the multiplex, no 448-bp product of @entity14851 was observed in the clinical samples of either group. The 695bp products of @entity1327 were observed only in 64.6% of the @entity1330 and @entity791 group. This primer set was negative for two standard strains. The specificity based on the negative samples from the CTL/ @entity791 group was 98.5% in both primer sets. CONCLUSIONS: These data suggest that the CN4/CN5 primer set was highly sensitive for the identification of @entity1327 / @entity14851 species complex in cerebrospinal fluid samples from @entity1 with clinical suspicion of @entity1330 .
