Cardiac dysfunction in the Goto-Kakizaki @entity35 . A model of XXXX .

@entity2175 @entity296 @entity6 @entity519 @entity1723 @entity460 @entity35 @entity67 @entity942
METHODS: Both isolated perfused hearts and isolated ventricular myocytes from GK and matched control @entity35 @entity35 hearts were studied. Percent myocyte twitch shortening (%TS) and corresponding intracellular @entity460 transients (indo-1 fluorescence ratio, R) were measured over a range of stimulation frequencies (0.5-2.5 Hz; 32 degrees C, n = 16-24 cells). In isolated Langendorff-perfused hearts, we measured systolic LV pressure (LVP(max)), left ventricular end diastolic pressure (LVEDP), maximal rate of LV pressure rise (LV dP/dt(max)) and fall (LV dP/dt(min)) and isovolumic LV relaxation (exponential time constant, T) both at baseline and during brief (10 minutes) @entity519 . RESULTS: The %TS and corresponding indo-1 R were similar between GK and control myocytes at all stimulation frequencies (e.g. at 2.5 Hz: % TS = 8.6 +/- 0.77 and 8.2 +/- 0.19; R = 0.19 +/- 0.009 and 0.18 +/- 0.018, GK and control respectively, P = NS). Similarly, there were no significant differences in baseline LVP(max) (129 +/- 6.2 and 135 +/- 9.6 mmHg; GK and control respectively, P = NS), LV dP/dt(max) (3169.5 +/- 165.80 and 3390.6 +/- 232.60 mmHg/s; GK and control respectively, P = NS), LV dP/dt(min) or T (24 +/- 0.7 and 25 +/- 0.6 ms, GK and control respectively, P = NS). During 10 min @entity519 , LV dP/dt(max) decreased significantly more, and LVEDP and T increased significantly more, in GK compared to control hearts (LV dP/dt(max): 668.90 +/- 32.8 versus 1027.10 +/- 84.0 mmHg/s; LVEDP: 21.4 +/- 4.3 versus 11.6 +/- 0.6 mmHg; T: 102 +/- 13.8 versus 56 +/- 3.0 ms; GK versus control respectively; all P < 0.05). These abnormalities in GK hearts were reversed with acute addition of insulin (0.01 i. u./ml) to the perfusion buffer. CONCLUSION: The GK model of @entity2175 a mild @entity296 evident as exaggerated @entity67 during @entity519 . The mechanism is likely to involve @entity942 . Experimental study of cardiac function in the @entity6 heart has focussed mostly on models of @entity1723 . We studied cardiac function in the Goto-Kakizaki (GK) @entity35 , an inbred model of spontaneous non-obese, @entity2175 .
