Biphasic @entity51549 administration and Schwann cell transplantation for repair after cervical contusive XXXX .

@entity1566 @entity51549 @entity35 @entity15882
UNASSIGNED: Schwann cells (SCs) hold promise for @entity1566 ( @entity1566 ) repair; however, there are limitations for its use as a lone treatment. We showed that acute inhibition of the phosphatase and tensin homolog deleted on chromosome ten (PTEN) by @entity51549 ( @entity15882 ) was neuroprotective and enhanced function following cervical hemicontusion @entity1566 . We hypothesized that combining acute @entity15882 therapy and delayed SC engraftment would further improve neuroprotection and recovery after cervical @entity1566 . Adult female Sprague-Dawley (SD) @entity35 were randomly sorted into 5 groups: sham, vehicle, @entity15882 , SC transplantation, and @entity15882 +SC transplantation. SCs were isolated from adult green fluorescent protein (GFP)-expressing SD @entity35 (GFP-SCs). 200 g/kg @entity15882 (pic) was administered intraperitoneally (IP) twice daily for 7days post- @entity1566 in @entity15882 -treated groups. GFP-SCs (1*10(6) in 5 l medium) were transplanted into the lesion epicenter at the 8th day post- @entity1566 . Forelimb function was tested for 10weeks and histology was assessed. @entity15882 alone significantly reduced lesion (by 40%, p<0.05) and cavitation (by 65%, p<0.05) and improved functional recovery (p<0.05) compared to injury alone. The combination promoted similar neuroprotection (p<0.01 vs. injury); however, GFP-SCs alone did not. Both SC-transplanted groups exhibited remarkable long-term SC survival, SMI-31(+) axon ingrowth and RECA-1(+) vasculature presence in the SC graft; however, @entity15882 +SCs promoted an 89% greater axon-to-lesion ratio than SCs only. We concluded that @entity15882 likely contributed largely to the neuroprotective and functional benefits while SCs facilitated considerable host-tissue interaction and modification. The combination of the two shows promise as an attractive strategy to enhance recovery after @entity1566 .
