Characteristics of immunogenic and tolerogenic dendritic cells within the arterial wall in XXXX and in vitro.

@entity1 @entity558 @entity404 @entity6 @entity731 @entity8730 @entity13128 @entity4155 @entity2533 @entity9536 @entity432 @entity2935 @entity688 @entity4896
AIM: To investigate the characteristic of mature dendritic cell ( @entity13128 ) and tolerogenic dendritic cell (TDC) in @entity1 lower limb @entity432 ( @entity432 ) and @entity6 foot and in vitro. METHODS: 58 @entity1 @entity432 and @entity6 foot arterial specimens were collected from surgical operation and autopsy. Immunohistochemical and Western blotting method were used to examine the distribution and the content of @entity8730 and @entity4896 positive reaction @entity13128 and @entity9536 and @entity13128 -SIGN positive reaction TDC. Furthermore, bone marrow-derived DCs were induced by rmGM-CSF and rmIL-4 in the presence or absence of @entity558 in vitro. The percent of @entity2935 (+) @entity9536 (+)TDC and @entity2935 (+) @entity8730 (+) @entity13128 were analyzed by flow cytometry. The effects of TDC and @entity13128 on T lymphocytes were analyzed by the @entity4155 level, the percent of Th17, and @entity4155 mRNA expression. RESULTS: Immunogenicity @entity13128 was heavily found in intima plaque and around the small vessel of adventitia on artherosclerosis aorta of lesion group, and was positively correlated to the progress of the disease. However, there were low expression of TDC and was negatively correlated to the progress of the disease. Meanwhile, we found that there is a close relationship between high glucose and disease progression. TDC expressed high levels of @entity688 and @entity2533 and down-regulated the percent of @entity404 (+) @entity4155 (+) Th17, @entity4155 mRNA and the level of @entity4155 in vitro. CONCLUSION: TDC and @entity13128 are assembled in the process of @entity432 , and the progression of the disease might be aggravated by @entity13128 -maturation. High glucose might closely relate to the progression of @entity731 .
