Evaluation of Spinal Fusion Using Bone Marrow Derived Mesenchymal Stem Cells with or without XXXX .

@entity35 @entity26684 @entity692
OBJECTIVE: In this study, the authors assessed the ability of @entity35 bone marrow derived mesenchymal stem cells (BMDMSCs), in the presence of a growth factor, @entity26684 ( @entity26684 ) and @entity692 , to act as a scaffold for posterolateral spinal fusion in a @entity35 model. METHODS: Using a @entity35 posterolateral spine fusion model, the experimental study comprised 3 groups. Group 1 was composed of 6 animals that were implanted with 0.08 gram @entity692 only. Group 2 was composed of 6 animals that were implanted with 0.08 gram @entity692 containing 1 x 10(6)/ 60 microL @entity35 of BMDMSCs. Group 3 was composed of 6 animals that were implanted with 0.08 gram @entity692 containing 1 x 10(6)/ 60 microL of @entity35 BMDMSCs and @entity26684 1 microG to induce the bony differentiation of the BMDMSCs. @entity35 were assessed using radiographs obtained at 4, 6, and 8 weeks postoperatively. After sacrifice, spines were explanted and assessed by manual palpation, high-resolution microcomputerized tomography, and histological analysis. RESULTS: Radiographic, high-resolution microcomputerized tomographic, and manual palpation revealed spinal fusion in five @entity35 (83%) in Group 2 at 8 weeks. However, in Group 1, three (60%) @entity35 developed fusion at L4-L5 by radiography and two (40%) by manual palpation in radiographic examination. In addition, in Group 3, bone fusion was observed in only 50% of @entity35 by manual palpation and radiographic examination at this time. CONCLUSION: The present study demonstrates that 0.08 gram of @entity692 with 1 x 10(6)/ 60 microL @entity35 of BMDMSCs induced bone fusion. @entity26684 , added to differentiate primitive 1 x 10(6)/ 60 microL @entity35 of BMDMSCs did not induce fusion. Based on histologic data, @entity26684 appears to induce fibrotic change rather than differentiation to bone by 1 x 10(6)/ 60 microL @entity35 of BMDMSCs.
