[ XXXX level in ovarian follicular fluid or serum as a predictor of successful fertilization].

@entity1 @entity5427 @entity455 @entity1255 @entity5274 @entity52 @entity393
OBJECTIVES: There are some data concerning @entity1255 influence on fertilization and early embryogenesis, especially in @entity1 experiencing recurrent @entity393 (RSA). However, limited data support hypothesis that the ovum might be exposed to high @entity1255 concentration what may be important in egg-sperm interactions, for example during @entity52 ( @entity52 ) cycle. DESIGN: Therefore, the aim of our study was to determine fasting total @entity1255 concentration in follicular fluid or serum of @entity1 experiencing reproductive failure after spontaneous or in-vitro fertilization. MATERIALS AND METHODS: Eight nonpregnant RSA @entity1 , 8 normal healthy @entity1 with previous successful pregnancy outcome and 15 @entity1 undergoing @entity52 (6 with unexplained infertility-UI, 6 with male factor-MF, 3 with @entity5274 ). Total fasting @entity1255 level concentrations were established by ELISA method (Axis @entity1255 EIA, @entity5427 ) in serum of RSA and normal healthy @entity1 or ovarian follicular fluid of @entity1 undergoing @entity52 . RESULTS: Mean fasting total @entity1255 concentration in study group were as follows: RSA-18.63 mumol/L +/- 6.67, Normal-13.98 mumol/L +/- 6.62, UI-20.62 mumol/L +/- 8.19, MF-22.60 mumol/L +/- 7.87, TO-36.75 mumol/L +/- 13.26. We found that RSA @entity1 have had significantly higher serum @entity1255 concentration when compared to normal healthy @entity1 (P < 0.05). Among @entity1 undergoing @entity52 , those with following @entity52 success had not significantly lower @entity1255 level when compared to those with @entity52 failure (22.81 mumol/L +/- 11.27 vs. 24.54 mumol/L +/- 9.50, P > 0.05). CONCLUSIONS: Our preliminary data suggest that high @entity1255 level may negatively influence pregnancy outcome following natural or in-vitro fertilization. It cannot be excluded that elevated @entity1255 concentrations contribute to defective @entity455 during early stages of gestation.
