IbeA, the invasin of Adherent-Invasive XXXX (AIEC) mediates interaction with intestinal epithelia and macrophages.

@entity1 @entity1357 @entity19 @entity4897 @entity281 @entity30 @entity526
UNASSIGNED: Adherent-invasive @entity526 (AIEC) are a group of isolates from the intestinal mucosa of @entity30 @entity1 that can invade intestinal epithelial cells (IECs) or macrophages and survive and/or replicate within. We have identified the ibeA gene in the genome of AIEC strain NRG857c and report now the contribution of IbeA to the interaction of AIEC with IECs, macrophages and colonization of the @entity19 intestine. An ibeA deletion mutant (  ibeA) was constructed and the in vitro effect on AIEC adhesion and invasion of non-polarized and polarized Caco-2 cells, adhesion and transcytosis of M-like cells, intracellular survival in @entity1357 macrophages, and contribution to intestinal colonization of the @entity4897 @entity19 model of @entity281 , was evaluated. A significant reduction in invasion was observed with the ibeA mutant in Caco-2 and M-like cells, while adhesion was not affected. Complementation of the mutant re-established Caco-2 invasive phenotype to wt levels. Reduction in invasion did not significantly affect transcytosis through M-like cells at early time points. The absence of ibeA significantly affected AIEC intra-macrophage survival up to 24 h post- @entity281 . No significant changes associated with IbeA were found in AIEC colonization across the @entity19 gastrointestinal tract, but a slight reduction of IFN-y was observed in the cecum of @entity19 infected with the ibeA mutant. Additionally, a decrease in the pathology scores was observed in the ileum and cecum of @entity19 infected with the ibeA mutant. Our data supports the function of IbeA in AIEC invasion process, macrophage survival and inflammatory response in the @entity19 intestine.
