Effects of XXXX on the morphology of cultured @entity35 granulosa cells.

@entity1 @entity1420 @entity53 @entity134 @entity3695 @entity3566 @entity35
Morphological changes were correlated with biochemical data induced by @entity3695 (PRL) in cultured @entity35 granulosa cells from large preovulatory follicles. Biochemical results indicated that PRL exerted a significant dose-dependent inhibition in gonadotrophin-induced secretion of @entity1420 and @entity53 . PRL alone failed to affect basal steroidogenic secretion. In parallel morphological experiments, using phase-contrast microscopy, untreated and 100 ng/ml PRL-treated cells appeared as a monolayer of flattened, fibroblast-like cells. Upon exposure to 0.4 IU/ml @entity1 chorionic gonadotrophin ( @entity3566 ), aggregates of rounded, epithelioid-shaped cells were formed. The addition of PRL to @entity3566 in the same doses minimized the changes induced by @entity3566 . Similarly, electron microscopy of untreated and PRL-treated cultures revealed flat cells devoid of microvilli, with evenly dispersed microfilaments. The addition of @entity3566 caused rounding of the cells and was accompanied by the appearance of microvilli and by pronounced @entity134 -producing organelles. Bundles of microfilaments were noted at the cell periphery. PRL added to @entity3566 caused a reduction of the @entity3566 effects, and the cell morphology was intermediate to that seen in untreated and @entity3566 -treated cultures. The finding that PRL can prevent or minimize morphological changes caused by @entity3566 in @entity35 cultured granulosa cells correlates with the biochemical changes induced by PRL, and supports the concept that PRL is a modulator of gonadotrophic action in the ovary.
