XXXX cells exposed to ionizing radiation: Structural and biochemical aspects.

@entity1 @entity5568 @entity5 @entity2842 @entity3460 @entity2073
UNASSIGNED: Acute or chronic exposure to ionizing radiation is a factor that may be hazardous to health. It has been reported that exposure to low doses of radiation (less than 50 @entity2073 /year) and subsequently exposure to high doses produces greater effects in @entity1 . It has been reported that @entity1 who have been exposed to low doses of radiation (less than 50 @entity2073 /year) and subsequently are exposed to high doses, have greater effects. However, at a molecular and biochemical level, it is an unknown alteration. This study, analyzes the susceptibility of a biological system (HeLa ATCC @entity2842 @entity1 @entity5 cell line) to ionizing radiation (6 and 60 @entity2073 /90s). Our research considers multiple variables such as: total protein profile, mitochondrial metabolic activity (XTT assay), cell viability ( @entity3460 exclusion assay), cytoskeleton (actin microfilaments), nuclei (DAPI), and genomic DNA. The results indicate, that cells exposed to ionizing radiation show structural alterations in nuclear phenotype and aneuploidy, further disruption in the tight junctions and consequently on the distribution of actin microfilaments. Similar alterations were observed in cells treated with a genotoxic agent (200 M @entity5568 /1h). In conclusion, this multi-criteria assessment enables precise comparisons of the effects of radiation between various line cells. However, it is necessary to determine stress markers for integration of the effects of ionizing radiation.
