Evidence for a family of four immunochemically related isozymes of cytochrome P-450 purified from untreated XXXX .

@entity95 @entity72903 @entity35 @entity13234 @entity72902
Hepatic microsomal cytochromes @entity72902 have recently been purified to electrophoretic homogeneity from untreated adult @entity35 and identified as distinct isozymes [Ryan et al. (1984) J. Biol. Chem. 259, 1239-1250; Haniu et al. (1984) Arch. Biochem. Biophys. 235, 304-311]. In Ouchterlony double-diffusion plates, as well as enzyme-linked immunosorbent assays and Western blots, purified immunoglobulin G (IgG) raised in @entity95 against cytochromes P-450f or P-450g show strong cross-reactions with the heterologous protein, indicating apparent partial identity. Anti- @entity13234 also show strong cross-reactivity with cytochromes @entity72903 but not with five previously characterized @entity35 hepatic cytochromes, P-450 (i.e., P-450a-P-450e), indicating a high degree of immunochemical and structural relatedness among cytochromes @entity72902 .
