Sex related differences in XXXX choroid plexus and cerebrospinal fluid: a cDNA microarray and proteomic analysis.

@entity35 @entity16 @entity2846 @entity2383
UNASSIGNED: The @entity2846 ( @entity2846 ) is a unique structure in the brain that forms an interface between the peripheral blood and the cerebrospinal fluid (CSF) which is mostly produced by the @entity2846 itself. Since the @entity2846 transcriptome is regulated by the sex hormone background, the goal of this study was to compare gene/ protein expression profiles in the @entity2846 and CSF from male and female @entity35 to better understand sex related differences in @entity2846 functions and brain physiology. We used data previously obtained by cDNA microarrays to compare the @entity2846 transcriptome between male and female @entity35 , and complemented these data with the proteomic analysis of the CSF of castrated and sham-operated males and females. Microarray analysis showed that 17128 and 17002 genes are expressed in the male and female @entity2846 , what allowed the functional annotation of 141 and 134 pathways, respectively. Among the most expressed genes, canonical pathways associated with @entity2383 and oxidative phosphorylation were the most prominent, while the most relevant molecular and cellular functions annotated were protein synthesis, cellular growth and proliferation, cell death and survival, molecular transport and protein trafficking. No significant differences were found between males and females regarding these pathways. Seminal functions of the @entity2846 differentially regulated between sexes were the circadian rhythm signalling, and several canonical pathways related to stem cell differentiation, metabolism, and the barrier function of the @entity2846 . The proteomic analysis identified 5 down-regulated proteins in the CSF samples from male @entity35 compared with females and 7 proteins exhibiting marked variation in the CSF of gonadectomised males in comparison to sham animals, whereas no differences were found between sham and ovariectomized females. These data clearly show sex related differences in the @entity2846 gene expression and in the CSF protein composition that may impact on @entity16 . This article is protected by copyright. All rights reserved.
