Stereotactic injection of cerebrospinal fluid from anti- @entity3391 receptor XXXX into @entity35 dentate gyrus impairs @entity3391 receptor function.

@entity1 @entity198 @entity146 @entity75 @entity3391 @entity6806 @entity6795 @entity14752 @entity302 @entity35 @entity2414 @entity7667
UNASSIGNED: @entity14752 is increasingly recognized in @entity1 with otherwise unexplained @entity75 with @entity302 . Among these, patients with anti- @entity3391 receptor (NMDAR) @entity198 present @entity302 , @entity2414 , and @entity146 symptoms. However, the functional consequences of such autoantibodies are poorly understood. In order to investigate the pathophysiology of this disease, we stereotactically injected either cerebrospinal fluid (CSF) from three anti-NMDAR @entity198 @entity1 or commercially available anti- @entity6806 into the dentate gyrus of adult female @entity35 . Control animals were injected with either CSF obtained from three @entity302 @entity1 ( @entity7667 , @entity302 , focal @entity6795 ) lacking anti-NMDAR or saline. Intracellular recordings from dentate gyrus granule cells showed a significant reduction of the NMDAR-evoked excitatory postsynaptic potentials (NMDAR-EPSPs) in animals treated with anti-NMDAR. As a consequence of this, action potential firing in these cells by NMDAR-EPSPs was significantly impaired. Long-term potentiation in the dentate gyrus was also significantly reduced in @entity35 injected with anti-NMDAR as compared to control animals. This was accompanied by a significantly impaired learning performance in the Morris water maze hidden platform task when the animals had been injected with anti-NMDAR antibody-containing CSF. Our findings suggest that anti-NMDAR lead to reduced NMDAR function in vivo which could contribute to the @entity2414 found in patients with anti-NMDAR @entity198 .
