The effect of XXXX on microfilariae of Litomosoides carinii in vitro and in vivo.

@entity6566 @entity6567 @entity1219 @entity35 @entity298
Culture-derived Litomosoides carinii microfilariae (MFF) were used in in vitro and in vivo systems to investigate the effect of @entity6566 ( @entity6567 ) on these MFF. In vivo: Male @entity35 , Mastomys natalensis, all of the same age, were injected intrathoracically (12) or intraperitoneally (36) with 10(3) or 10(4) MFF. After 30 min one half of each group of @entity35 was given @entity6567 per os. At 30, 60, and 120 min after @entity6567 administration, two @entity35 from the treated and two from the untreated group were bled and killed. The pleural or peritoneal cavities were rinsed with warm saline (0.15 M @entity1219 ) to recover MFF. In both the intrathoracic and intraperitoneal experiments, equal numbers of MFF were recovered from treated and control @entity35 at 30 and 120 min. However, at 60 min 85.5% fewer were recovered from the treated than from the nontreated animals. MFF were not found in the blood. In vitro: MFF were added to tissue culture dish wells (Linbro Div., Flow Labs, Hamden, Conn) prepared as follows: @entity6567 -Serum (serum from normal @entity35 given @entity6567 at 500 mg/kg), @entity6567 + Serum (serum with added @entity6567 ), serum only, RPMI 1640 only, and RPMI 1640 + @entity6567 . Furthermore, the five treatments were prepared either with or without unstimulated @entity298 ( @entity298 ) cells. At 30 min in the @entity6567 -Serum wells 45% of the MFF had adherent @entity298 cells; in the remaining wells these cells adhered to 11% or fewer MFF. We interpret the aforementioned phenomena as representing the first step in the trapping and elimination of MFF after @entity6567 treatment of L. carinii-infected M. natalensis.
