Characterization and Sub-cellular Localization of XXXX -Binding Proteins Isolated from @entity1 Hepatic Stellate Cells.

@entity1 @entity52108 @entity82 @entity20176 @entity1521 @entity32656 @entity72240 @entity38806 @entity40329 @entity2533 @entity68187 @entity26243
UNASSIGNED: Although the expression levels of total @entity26243 -binding proteins (GNBPs) were up-regulated significantly in @entity1 hepatic stellate cells (HSCs) activated with transforming growth factor-b1( @entity2533 ), yet little is known about the precise types, distribution and sub-cellular localization of the GNBPs in HSCs. Here, 264 GNBPs from the activated HSCs and 257 GNBPs from the quiescent HSCs were identified and annotated. A total of 46 GNBPs were estimated to be significantly up-regulated and 40 GNBPs were estimated to be significantly down-regulated in the activated HSCs. For example, the GNBPs (i.e. @entity52108 , @entity38806 , and @entity40329 ) responsible for the regulation of protein binding were up-regulated, and those (i.e. @entity72240 , @entity68187 , and @entity20176 ) responsible for the regulation of protein binding were down-regulated in the activated HSCs. The motifs of the isolated GNBPs showed that @entity1521 residue had the maximum preference in consensus sequences. The western blotting showed the expression levels of @entity38806 , and @entity32656 were significantly up-regulated, while, the expression level of CLIC1(B5) was down-regulated in the activated HSCs and liver @entity82 tissues. Moreover, the GNBPs were sub-localized in the Golgi apparatus of HSCs. In conclusion, the precision alteration of the GNBPs referred to pathological changes in liver @entity82 / @entity82 may provide useful information to find new molecular mechanism of HSC activation and discover the biomarkers for diagnosis of liver @entity82 / @entity82 as well as development of new anti-fibrotic strategies.
