[Effects of Shenfu injection on @entity66104 expression and neuronal apoptosis in the cerebral cortex of neonatal @entity35 with XXXX ].

@entity63 @entity75 @entity66104 @entity460 @entity35 @entity579
OBJECTIVE: To examine the expression of @entity66104 ( @entity66104 ) and the changes of intracellular free @entity460 and neuronal apoptosis in the cerebral cortex of neonatal @entity35 with @entity75 ( @entity75 ), and to investigate the intervention effects of Shenfu injection. METHODS: Seven-day-old @entity35 were randomly assigned to three groups: control, @entity63 ( @entity63 ) and Shenfu-treated. Each group (n=50) was subdivided into 5 groups sacrificed at 3, 6, 12, 24 and 72 hours. @entity35 models of @entity75 were prepared according to the @entity579 's method. @entity35 in the control group only underwent the separation of right common carotidartery. Shenfu injection was administered by intraperitoneal injections right after @entity63 insults and then once daily at a dosage of 10 mL/kg for 3 days in the Shenfu-treated group. The expression of @entity66104 in the cerebral cortex was detected by RT-PCR and Western blot. The free @entity460 concentrations were determined under a fluorescent microscope. The apoptosis rate was measured by the flow cytometry. RESULTS: Compared with the control group, the expression levels of @entity66104 in the @entity63 and the Shenfu-treated groups were obviously up-regulated (P<0.05), and the expression levels of @entity66104 in the Shenfu-treated group were notably higher than those in the @entity63 group (P<0.05) at all time points. The concentrations of intracellular free @entity460 and the apoptosis rate of neurons in the cerebral cortex in the Shenfu-treated group were significantly reduced compared with those in the @entity63 group (P<0.05), but increased significantly compared with those in the control group at all time points (P<0.05). CONCLUSIONS: Shenfu injection may have neuroprotective effects against @entity75 by up-regulation of @entity66104 expression and relief of @entity460 overload.
