Physiological characterization of the XXXX EndoC-bH1 b-cell line.

@entity1 @entity268 @entity972 @entity4307 @entity14225 @entity35 @entity19 @entity9640 @entity5217 @entity1922 @entity14224 @entity35316 @entity6525 @entity1928 @entity1365 @entity141 @entity857 @entity9898 @entity413 @entity855
UNASSIGNED: In the new @entity1 EndoC-bH1 b-cell line, a detailed analysis of the physiological characteristics was performed. This new @entity1 b-cell line expressed all target structures on the gene and protein level, which are crucial for physiological function and insulin secretion induced by @entity413 and other secretagogues. @entity413 influx measurements revealed an excellent uptake capacity of EndoC-bH1 b-cells by the @entity1365 and @entity35316 @entity413 transporters. A high expression level of @entity14224 enabled efficient @entity413 phosphorylation, increasing the @entity855 / @entity857 ratio along with stimulation of insulin secretion in the physiological @entity413 concentration range. The EC50 value of @entity413 for insulin secretion was 10.2 mM. Mannoheptulose, a specific @entity14224 inhibitor, blocked @entity413 -induced insulin secretion (GSIS).The nutrient insulin secretagogues @entity4307 and @entity6525 also stimulated insulin secretion, with a potentiating effect of @entity9640 . The @entity14225 @entity972 channel blocker @entity5217 and @entity9898 , an opener of the voltage-sensitive @entity141 channel significantly potentiated GSIS. Potentiation of GSIS by @entity1922 and @entity1928 went along with a strong stimulation of @entity268 generation. In conclusion, the new @entity1 EndoC-bH1 b-cell line fully mirrors the analogous physiological characteristics of primary @entity19 , @entity35 and @entity1 b-cells. Thus, this new @entity1 EndoC-bH1 b-cell line is very well suited for physiological b-cell studies.
