Tear film proteins deposited on high water content contact lenses identified with two-dimensional gel electrophoresis and XXXX .

@entity1 @entity2194 @entity939 @entity575 @entity3928 @entity2975
PURPOSE: Tear film proteins adhere to the surface of contact lenses (CLs). While the proteins in the tears have been extensively studied with various proteomic techniques, adhered proteins to CLs are less studied. In this pilot study, we have separated proteins with 2D gel electrophoresis prior to the conventional @entity3928 ( @entity3928 ) in order to analyse the deposited proteins on hydrogel CLs from myopic @entity1 . METHODS: pHEMA and @entity939 hydrogel CLs worn by 3 @entity1 for different time lengths were analysed. After wear, the CLs were frozen at -20  C. Proteins were extracted in lysis buffer, separated on 12% @entity2975 gels and @entity575 -stained. Protein spots were excised and identified with liquid chromatography - tandem @entity3928 . RESULTS: Deposited proteins were extracted with a yield of 26-66   g and separated by 2D gel electrophoresis. The @entity575 -stained gels showed similar protein patterns independent of the @entity1 , hydrogel type and wear time. Seventy-two spots were analysed with @entity3928 , representing at least 12 different tear film proteins or protein fragments. CONCLUSIONS: Deposited tear film proteins from a single set of CLs worn for 1  day can successfully be analysed first with 2D gel electrophoresis and subsequently with @entity3928 , thus making examination of individual @entity1 possible. The protein composition appeared homogeneous between the test @entity1 which is a necessity for additional comparison analysis. The molecular masses of the identified proteins indicate that protein degradation occurs only as a minor event. Myopic @entity1 were investigated in this pilot study, but the combined techniques can easily be applied to other @entity2194 .
