Localization of IGF proteins in various stages of ovarian follicular development and modulatory role of IGF-I on granulosa cell XXXX production in water buffalo (Bubalus bubalis).

@entity24396 @entity1420 @entity72123 @entity72125 @entity72126 @entity72127 @entity72128 @entity46670 @entity72124 @entity479
UNASSIGNED: The present study aimed to determine the expression of insulin like growth factor (IGF) genes in the bubaline ovarian follicles and modulatory role of IGF-I on @entity1420 production from granulosa cells (GC) of pre-ovulatory follicle in vitro. According to size, follicles were classified into four groups: GI (small), GII (medium), GIII (large) and @entity24396 (preovulatory). All IGF genes were expressed in both GC and theca interna (TI) cells. The relative expression of IGF-I and IGF receptor I (IGFR-I) genes increased with follicle size and was greatest in the pre-ovulatory follicle (P<0.05). Expression of @entity46670 and IGFR-II genes was minimal in GC but was readily detected in TI cells. In TI cells, the gene expression was greater in medium and large as compared to small and pre-ovulatory follicles. The expression of all binding protein (IGFBP) genes was detected in both GC and TI cells. Expression of @entity72123 gene increased with follicle size and was greatest in pre-ovulatory follicles (P<0.05). The expression of @entity72124 and @entity72125 was less in pre-ovulatory follicles but expression of @entity72126 and @entity72127 genes were greater at this stage. The GC culture was conducted for three time durations and with three doses of IGF-I. Expression of steroidogenic genes (StAR, CYP11A1, HSD3B) and @entity1420 concentration were increased in a dose and time dependent fashion. The present study, therefore, provided evidence of an autocrine/paracrine role of IGFs in follicular development and a stimulatory role of @entity72128 in @entity479 production in GC of preovulatory follicles in the bubaline species.
