@entity379 -treated Dental Pulp Stem Cells Promote XXXX Mesenchymal Stem Cell Migration In  Vitro.

@entity1 @entity32 @entity70717 @entity622 @entity25296 @entity379 @entity70716
INTRODUCTION: @entity32 disrupts dental pulp regeneration by disintegrating the recruitment process of progenitors for repair. Bone marrow-derived mesenchymal stem cells (BM-MSCs) share the common features with dental pulp stem cells (DPSCs). The aim of the study was to investigate the migration of BM-MSCs toward DPSCs in response to inflammatory chemoattractants. Additionally, our studies also delineated the signaling mechanisms from BM-MSCs in mediating the proliferation and differentiation of DPSCs in  vitro. METHODS: @entity1 DPSCs and BM-MSCs between passages 2 and 4 were used and were grown in odontogenic differentiation medium. Mineralization was determined by @entity622 staining analysis. Migration was assessed using crystal violet staining in cells grown in Boyden chamber Transwell inserts (Corning Inc Foundation, Tewksbury, MA). The mineralization potential of DPSCs was evaluated using alkaline phosphatase activity assay. Real-time polymerase chain reaction analysis was performed to assess the gene expression profile of chemokine ( @entity70716 , 10, 11, 12, 14, and 16; stromal cell-derived factor (SDF) a; vascular endothelial growth factor; and fibroblast growth factor. RESULTS: @entity379 (FN-y) treatment significantly abrogated the differentiation potential of DPSCs as shown by using @entity622 red and alkaline phosphatase activity analysis. An increase in the migration of BM-MSCs was documented when cocultured with IFN-y-treated DPSCs. RNA expression studies showed an increase in the levels of @entity25296 and Cxcl12 in BM-MSCs when cocultured with IFN-y-treated DPSCs. Additionally, an up-regulation of proangiogenic factors vascular endothelial growth factor and fibroblast growth factor were observed in DPSCs exposed to IFN-y. CONCLUSIONS: Our findings indicate that inflamed IFN-y-treated DPSCs release factors (presumably @entity70717 ) that contribute to the homing of MSCs. This model might provide a potential research tool for studying MSC-DPSC cross talk and for future studies involving the recruitment and sustainability of progenitor stem cells sustaining the inflammatory cascade to treat pulp @entity32 .
