An enzymatic assay for XXXX concentration in human serum and plasma.

@entity4990 @entity6579 @entity11834 @entity2434 @entity273 @entity1877 @entity15002 @entity23134 @entity1718 @entity4291
OBJECTIVES: Several methods for measuring @entity6579 ( @entity15002 ) concentrations have been reported. However, these methods are not practical because they are either too complicated and/or too time-consuming for @entity15002 determinations in human serum and plasma. DESIGN AND METHODS: We have developed a new enzymatic @entity15002 assay, which uses lysophospholipase, @entity11834 phosphodiesterase and @entity1877 oxidase, and which determines the quantities of @entity273 generated in the presence of peroxidase using an oxidative chromogenic reagent and @entity23134 . RESULTS: Various samples were mixed with @entity15002 assay reagents, and their changes in absorbance were measured. The present method produced a linear calibration line between @entity15002 concentration and absorbance change. It also measured only @entity15002 , and not other phospholipids such as @entity2434 , @entity4291 and @entity4990 . The within-run and between-run coefficients of variation were 0.3-0.7% and 0.7%, respectively. The recovery of exogenous @entity15002 added to control serum was 99.5-102.1%. The correlation coefficient obtained in a comparison with a method for analyzing @entity1718 was 0.9122. CONCLUSIONS: The present method is simple, specific for @entity15002 , and can be applied with an automatic analyzer. It may also be useful for further studies of the biological functions of @entity15002 as well as clinical applications in various disorders.
