Mast cell-macrophage dynamics in modulation of XXXX in skin.

@entity778 @entity1750 @entity19 @entity164 @entity7643 @entity779
@entity778 causes @entity779 , @entity7643 , or @entity7643 . Mast cells have been speculated to play a role in DENV disease although their precise roles are unclear. In this study, we used mast cell-deficient Kit(W-sh/W-sh) @entity19 to investigate the involvement of mast cells after intradermal @entity778 . An approximately two- to three-fold higher level of DENV NS3 antigen was detected at the skin inoculation site in DENV-infected Kit(W-sh/W-sh) @entity19 than in DENV-infected @entity164 ( @entity164 ) @entity19 (using a dose of 1 * 10(9) plaque-forming units/ @entity19 ). Moreover, as an indicator of heightened pathogenesis, a more prolonged bleeding time was observed in DENV-infected Kit(W-sh/W-sh) @entity19 than in @entity164 @entity19 . Monocytes/macrophages are considered to be important targets for @entity778 , so we investigated the susceptibility and chemokine response of DENV-infected peritoneal macrophages from Kit(W-sh/W-sh) and @entity164 @entity19 both ex vivo and in vivo. There was a tendency for higher @entity778 and higher secretion of @entity1750 ( @entity1750 ) from peritoneal macrophages isolated from Kit(W-sh/W-sh) @entity19 than those from @entity164 @entity19 . In vivo studies using intradermal inoculation of DENV showed about twofold higher levels of infiltrating macrophages and @entity1750 ( @entity1750 ) at the inoculation site in both mock control and DENV-inoculated Kit(W-sh/W-sh) @entity19 than in corresponding @entity164 @entity19 . In summary, compared with @entity164 @entity19 , Kit(W-sh/W-sh) @entity19 show enhanced @entity778 and macrophage infiltration at the skin inoculation site as well as increased DENV-associated bleeding time. The results indicate an intriguing interplay between mast cells and tissue macrophages to restrict DENV replication in the skin.
