Recurrent @entity32 induced by lipopolysaccharide (endotoxin) results in the formation of XXXX in vivo.

@entity32 @entity753 @entity4288 @entity75 @entity95 @entity2827 @entity165
Bacteria and viruses are suspected to induce @entity4288 ; however, most investigators have focused on coincidences rather than causal relationships. The aim of this work was to establish a @entity95 model in which the vessel reaction to local perivascular injection of defined bacterial products can be analyzed. A total of 23 @entity95 were randomized to four groups. Groups A and B were fed a normal diet, groups C and D were fed a @entity165 -enriched diet. Groups A and C were treated with a single perivascular injection of bacterial lipopolysaccharide (LPS, endotoxin) placed next to auricular, carotid and femoral arteries, and @entity2827 placed next to the contralateral arteries (control). Group B and D animals were treated with repeated perivascular injections over 90 days. Vascular tissues (n=116 treated segments of 23 @entity95 ) were analyzed using morphometry at histology, and using immunohistochemistry to detect macrophages, lymphocytes and vascular smooth muscle cells. LPS treatment resulted in transient focal intima thickening. After single LPS application, no increase in @entity75 formation was observed in comparison with controls (group C, lesion area index 0.031+/-0.012 vs 0.015+/-0.006, P=1.0). Repeated LPS application resulted in significant @entity75 formation compared with saline control (group D, lesion area index 0.148+/-0.049 vs 0.008+/-0.006, P=0.003) in @entity753 @entity95 . @entity32 in normocholesterolemic did not lead to @entity75 formation (intima media ratio 0.04+/-0.01 vs 0.04+/-0.007, P=1.0). Single perivascular administration of low-dose bacterial LPS resulted in transient focal intimal thickening, while significant increase in lesion formation occurred after repeated LPS application in @entity165 -fed animals. In conclusion, this animal model will allow the assessment of the impact of defined dosages of different bacterial pathogens onto the vascular wall in the context of atherogenesis. The @entity75 -promoting effect of repeated perivascular administration of LPS supports the hypothesis that bacterial pathogens may be involved in atherogenesis.
