MAGE-A expression clusters and antineoplastic treatment in XXXX .

@entity1 @entity135 @entity5 @entity39046 @entity957 @entity900 @entity1311 @entity20522 @entity43894 @entity6472 @entity1487 @entity72133 @entity1205 @entity320 @entity213
The nonsurgical treatment of @entity213 ( @entity213 ) usually consists of radiation and chemotherapy. In general, the treatment efficacy of chemotherapy in @entity135 is limited. Apart from the placenta, testis and fetal keratinocytes, @entity320 -associated antigens-A (MAGE-A) are only found in @entity5 . Even though their molecular role remains unclear, several subgroups have been found to contribute to resistance to different chemotherapeutic agents. In the present study, established @entity1 @entity957 cell lines were incubated with various concentrations of @entity900 , @entity1487 , @entity1311 , @entity1205 , cetuximab and panitumumab for 5, 10, 20 and 40 h. The treatment efficacy was measured dynamically by real-time cell analysis (RTCA). In addition, we determined the expression of all known MAGE-A subgroups ( @entity6472 to MAGE-A12, excluding pseudogene @entity72133 ) by reverse transcription quantitative polymerase chain reaction. Of note, one cell line showed only a marginal expression of MAGE-A antigens, whereas another cell line showed a distinct expression of almost all the MAGE-A subgroups. The expression pattern varied in the other cell lines. @entity20522 was the most highly expressed of all the subgroups, and @entity39046 could not be detected. With the exception of @entity43894 , -A8, -A9 and -A10, the expression levels differed significantly between the cell lines. Factor analysis suggested simplifying the MAGE-A expression level into two groups. Spearman's rank correlation revealed a significant association between MAGE-A expression and treatment efficacy for 20.8% (25/120) of the experiments. In 100% of these cases (25/25), Spearman's Rho revealed a positive correlation between clustered MAGE-A expression and poor treatment efficacy. Our data highlight the fact that higher a MAGE-A expression correlates with a poorer outcome of antineoplastic treatment. Clustered MAGE-A expression analysis may help to identify @entity1 who are at a higher risk of antineoplastic treatment failure.
