Long-term serial transplantation of different @entity1 @entity150 into NMRI nu/nu XXXX . Histologic, flow cytometric and growth studies.

@entity1 @entity19 @entity5 @entity1358 @entity150 @entity1606
Sixteen @entity1 @entity150 ( @entity150 ) were transplanted into NMRI nu/nu @entity19 with an acceptance rate of 62.5%. Six of the 16 (37.5%) @entity150 were successfully retransplanted up to 14 passages. Histologic grading and traumatization during surgery influenced the acceptance rate. Grade-I @entity5 were rarely accepted, unless they were obtained during open surgery, rather than by transurethral resection (TUR). Specimens taken by TUR or by electric cut during open surgery were almost never accepted. Identity of the transplanted @entity5 tissue with the original @entity1 @entity150 could be demonstrated by flow cytometry and by light microscopy through several passages in all accepted @entity5 . However, although the @entity5 retained their major structural features, their proliferative activity increased, particularly that of grade-I @entity5 . The @entity5 that were @entity1606 and could be subpassed had three characteristics not seen before in transplanted @entity1358 : @entity5 growth started after a delay which shortened with each further passage; growth rates of @entity5 only in their first passage correlated with the prognosis of the corresponding @entity1 , and with each further passage, @entity5 growth accelerated until a doubling time of about 1 week was observed. From then on, @entity5 growth was almost identical in all @entity5 . In contrast to our experience with transplanted @entity1358 , flow cytometric evaluation of the transplanted and further passaged @entity150 revealed changes in DNA index and an increase in proliferative rate and it seems that @entity150 were strongly influenced by host factors.
