[Effect of Gualou Xiebai Banxia decoction combined with Xuefu Zhuyu decoction on myocardial apoptosis and relevant protein expressions in miniature XXXX phlegm and blood stasis type @entity301 model].

@entity1707 @entity296 @entity301 @entity24247 @entity406 @entity1977 @entity453 @entity65721
There have been very few studies on the effect of Gualou Xiebai Banxia decoction combined with Xuefu Zhuyu decoction in inhibiting apoptosis in @entity296 caused by @entity301 . In this experiment, Gualou Xiebai Banxia decoction combined with-Xuefu Zhuyu decoction were used to intervene the miniature @entity1707 phlegm and blood stasis type @entity301 model, in order to observe the effect of the combined prescription on the myocardial apoptosis and the expressions of @entity24247 , @entity65721 , Caspase-3, Caspase-9 in the model. Totally 15 Chinese experimental miniature @entity1707 were adopted and randomly divided into the control group, the model group and the phlegm and stasis-treating group. The model group and the stasis-treating group were fed with high fat diets for two weeks, intervened with the @entity453 and then given drugs and high fat diets for eight weeks. The control group was fed with ordinary diets for 10 weeks, without the @entity453 . After the experiment, myocardia at the juncture of infracted areas were collected and made into @entity406 -fixed paraffin sections. The TDT-mediate @entity1977 nick end labeling (TUNEL) assay was used to detect the myocardial apoptosis. The immunohistochemistry (IHC) technique was applied to detect @entity24247 , @entity65721 , Caspase-3, Caspase-9 levels in myocardial tissues. According to the findings, the apoptosis indexes (AI) for the control group, the model group and the phlegm and stasis-treating group were 0.92%, 27.68%, 17.28%, respectively. The AI of the phlegm and stasis-treating group was significantly lower than that of the model group (P < 0.01). Compared with the model group, the phlegm and stasis-treating group showed significantly higher @entity24247 protein expression (P < 0.01) and lower @entity65721 , Caspase-3 and Caspase-9 protein expressions (P < 0.01). In conclusion, Gualou Xiebai Banxia decoction combined with Xuefu Zhuyu decoction have a significant protective effect against the myocardial apoptosis in miniature @entity1707 phlegm and blood stasis type @entity301 model.
