Evaluation of aspartic acid racemization ratios in the XXXX femur for age estimation.

@entity535 @entity79 @entity12161 @entity1
Levels of @entity12161 (D/L ratio) in cranial non-collagen proteins (acid-soluble peptide fractions) have been reported to increase with age. We isolated total @entity535 fractions from the femur and separately isolated acid-insoluble collagen fraction and acid-soluble peptide fractions; then D/L ratios were measured from each fraction by gas chromatography. We evaluated the applicability of their D/L ratios for age estimation based on their correlation coefficient. A sex-related difference was observed in the D/L ratio. In particular, aged females showed a low ratio, suggesting an association with @entity79 . In males, the D/L ratios of acid-soluble peptide fraction showed the highest correlation rate (r = 0.969) with age, and those of total @entity535 fraction showed the highest correlation rate (r = 0.633) with age in females. Without separation of male and female, the D/L ratios of total @entity535 fraction showed the highest value (r = 0.853). The D/L ratio of acid-soluble peptide fractions differed according to the size of bone powder particles, being higher for larger particle sizes. These results suggest that the application of D/L ratio from total @entity535 fraction is the most effective method for estimating age using the @entity1 femur. However, care is necessary when studing cadavers that might be females.
