Role of Mesenchymal Stem Cells Transfected With Vascular Endothelial Growth Factor in Maintaining Renal Structure and Function in XXXX with Unilateral Ureteral Obstruction.

@entity8 @entity82 @entity4295 @entity1695 @entity1421 @entity568 @entity17937 @entity720 @entity842 @entity810 @entity35 @entity1589 @entity10570
OBJECTIVES: Mesenchymal stem cells hold promise for @entity8 treatment. Vascular endothelial growth factor may heal tubule-interstitial @entity82 in unilateral @entity1695 by inhibiting epithelial-mesenchymal transition. We investigated the protective effect of vascular endothelial growth factor in transfected mesenchymal stem cells in unilateral @entity1695 -induced @entity8 in @entity35 . MATERIALS AND METHODS: Male @entity35 Albino @entity35 (32 @entity35 ; weight, 250-300 g) were divided into 4 equal groups: group 1, control; group 2, unilateral @entity1695 ; group 3, unilateral @entity1695 and mesenchymal stem cells; and group 4, unilateral @entity1695 and vascular endothelial growth factor-transfected mesenchymal stem cells. Vascular endothelial growth factor-transfected mesenchymal stem cells were administered intravenously before onset of unilateral @entity1695 . On day 14, the @entity35 were killed and kidneys were retrieved. @entity4295 , mononuclear cell infiltration, and interstitial @entity82 were evaluated in paraffin blocks. We evaluated green fluorescent protein-positive and vascular endothelial growth factor-positive cells; anti-inflammatory ( @entity1421 receptor) and interleukin 1 receptor antagonist), proinflammatory/anti-inflammatory ( @entity568 ), and proinflammatory ( @entity1589 ) cytokine expression levels; and levels of @entity842 ; transforming growth factor b1, @entity17937 , and @entity720 . RESULTS: Green fluorescent protein-positive cells were negative in the renal parenchyma in groups 1 and 2 and positive in groups 3 and 4. Vascular endothelial growth factor levels were significantly higher in group 4. Transforming growth factor b1, @entity842 , and @entity17937 levels were significantly higher in the unilateral @entity1695 than control group; however, in the study groups, these values were not significantly different from the unilateral @entity1695 group. In stem cell-transplanted tissue samples, @entity10570 antagonist, and @entity568 levels were elevated, but @entity1589 expression levels were low. Although there were significant differences for @entity4295 and @entity82 in group 2, there were significant reductions in @entity810 and @entity82 in groups 3 and 4. CONCLUSIONS: Systemic stem cells transplanted into the kidney protected against unilateral @entity1695 -induced @entity8 and @entity8 .
