Integrin ab1, avb, a6b effectors XXXX , @entity7242 and talin regulate carcinoma invasion and chemoresistance.

@entity7242 @entity1697 @entity5 @entity900 @entity25886 @entity48205 @entity55644 @entity20336
Ligand engagement by integrins induces receptor clustering and formation of complexes at the integrin cytoplasmic face that controls cell signaling and cytoskeletal dynamics critical for adhesion-dependent processes. This study searches for a subset of integrin effectors that coordinates both @entity5 cell invasion and resistance to the chemotherapeutic drug @entity900 in @entity1697 . Candidate integrin effectors were identified in a proteomics screen of proteins recruited to clustered integrin ab1, a(v)b or a(6)b @entity1697 . Proteins with diverse functions including microtubule and actin binding proteins, and factors involved in trafficking, transcription and translation were identified in @entity1697 integrin complexes. Knockdown of effectors in the @entity1697 @entity55644 cells revealed that @entity20336 , @entity48205 , @entity7242 and talin were required for invasion through Matrigel. Disruption of talin or @entity20336 by RNA interference increased resistance to @entity900 , whereas targeting @entity48205 , @entity7242 or @entity25886 reduced @entity55644 resistance to @entity900 . Analysis of the spreading of @entity55644 cells on collagen I and laminin I revealed that a decrease in @entity20336 or talin expression inhibited spreading on both matrices. Interestingly, a reduction in @entity25886 expression enhanced spreading on laminin I and inhibited spreading on collagen I. Reduction of @entity48205 , @entity7242 , talin or @entity25886 expression reduced @entity55644 proliferation by 30%. Proliferation was not affected by a reduction in @entity20336 expression. We conclude that @entity20336 , @entity7242 and talin function in both @entity1697 invasion and resistance to @entity900 .
