Mortality of XXXX epidermal keratinocytes in co-culture with oral squamous cell carcinoma cells.

@entity1 @entity15875 @entity1697 @entity894 @entity39 @entity6578
Normal @entity1 epidermal keratinocytes (NHEK) died after 5 days of co-culture with an @entity1697 cell line (HSC-4). However, the HSC-4 cells did not change in either mitotic character or shape. We analyzed the changes in the mitotic activity and DNA synthetic activity of NHEK in co-culture with the HSC-4 cells. The @entity6578 of the NHEK decreased gradually with the presence of @entity1 @entity15875 ( @entity15875 ) in the media. However, the @entity6578 ( @entity6578 ) of the HSC-4 cells did not change. The shape of the NHEK cells did not change during co-culture. The time changes of the @entity894 ( @entity894 ) measured by BrdU incorporation, and the @entity894 decreased in neither NHEK nor in HSC-4 cells in the presence of @entity15875 . Moreover, in co-culture with an anti- @entity15875 antibody, the @entity6578 of both NHEK and HSC-4 cells did not change. These results indicate that @entity15875 might not lead to apoptosis or @entity39 , but rather to cellular reproductive death in NHEK cells. However, HSC-4 cells might be more tolerant of @entity15875 , so that @entity15875 does not cause cellular reproductive death. @entity15875 does not diminish the DNA synthesis rate but decreases the mitotic activity in NHEK cells in vitro. @entity15875 diminishes neither the DNA synthesis rate nor the mitotic activity in HSC-4 cells.
