In vivo role of platelet-derived growth factor-BB in airway smooth muscle proliferation in XXXX lung.

@entity19 @entity565 @entity1373 @entity358 @entity3960
@entity3960 ( @entity3960 ) @entity1373 in @entity565 likely contributes considerably to functional changes. Investigating the mechanisms behind proliferation of these cells may lead to therapeutic benefit. Platelet-derived growth factor (PDGF)-BB is a well known @entity3960 mitogen in vitro but has yet to be directly explored using in vivo @entity19 models in the context of @entity3960 proliferation and airway responsiveness. To determine the in vivo influence of PDGF-BB on gene transcripts encoding contractile proteins, @entity3960 proliferation, and airway physiology, we used an adenovirus overexpression system and a model of chronic allergen exposure. We used adenovirus technology to selectively overexpress PDGF-BB in the airway epithelium of @entity19 . Outcome measurements, including airway physiology, real time RT-PCR measurements, proliferating cell nuclear antigen staining, and airway smooth muscle quantification, were performed 7 days after exposure. The same outcome measurements were performed 24 hours and 4 weeks after a chronic allergen exposure model. PDGF-BB overexpression resulted in airway hyperresponsiveness, @entity358 , increased airway smooth muscle cell numbers, positive proliferating cell nuclear antigen-stained airway smooth muscle cells, and a reduction in genes encoding contractile proteins. Chronic allergen exposure resulted in elevations in lung lavage PDGF-BB, which were observed in conjunction with changes in gene transcript expression encoding contractile proteins and @entity3960 proliferation. We demonstrate for the first time in vivo that PDGF-BB induces @entity3960 and changes in lung mechanics in @entity19 and that, during periods of allergen exposure changes in lung, PDGF-BB are associated with changes in airway structure and function.
