[Mitochondrial genetic apparatus functioning in XXXX spleen cells under radiation-induced apoptosis].

@entity16886 @entity19 @entity73339 @entity73338 @entity51731
An important role of mitochondria in the process of programmed cell death is widely accepted now. There is a set of nuclear-encoded mitochondrial proteins involved in this process. Apart from this, a mitochondrion contains its own genetic apparatus comprising mtDNA and replication, transcription, and translation systems. However, a mechanism of mitochondria genetic information realization under apoptosis-inducing conditions has been understood poorly. Here, using the real-time PCR technique the number of mitochondrial genes and their transcripts in @entity19 spleen cells after whole-body X-ray irradiation at the dosage of 10 Gy has been evaluated. During 5 h after the irradiation a nuclear DNA was subjected to fragmentation, whereas mtDNA remained intact. Moreover, in the course of time after irradiation the number of mtDNA copies increased threefold. A process of mtDNA transcription was more susceptible to the irradiation: in 1 h after exposure the number of @entity73338 , @entity51731 and @entity16886 gene transcripts were sharply decreased. In 24 and 72 h after the irradiation the amount of @entity73338 and @entity51731 transcripts was restored to the control values, while the @entity16886 one remained low; the number of @entity73339 transcripts was compared with the control within the whole period of observation. The difference in levels of mRNAs for the genes transcribed under the control of the same promoter for @entity19 to be grown both under normal conditions and after x-ray irradiation allows us to propose the existing a posttranscriptional mechanism which regulates expression of mitochondrial genes and provides different recovery rates for different mitochondrial transcripts at the development of apoptosis.
