Effects of lipopolysaccharide extracted from @entity3288 on bone formation and on the release of osteolytic mediators by fetal XXXX osteoblasts in vitro.

@entity2520 @entity19 @entity5780 @entity3288 @entity3971 @entity777 @entity1235 @entity842 @entity460
@entity3288 , a Gram-negative obligate anaerobic black-pigmented oral bacterium, belongs to a small group of microorganisms that is closely associated with the @entity2520 . Lipopolysaccharide (LPS), an outer membrane component, is one of the main virulence factors of this bacterium. The aim of this study was to examine the effects of Prev. intermedia lipopolysaccharide, extracted by the hot- @entity3971 -water method, on differentiation (alkaline phosphatase activity) and mineralisation ( @entity460 incorporation) of fetal @entity19 calvarial cells in vitro and to determine the release of the important osteolytic factors @entity842 , @entity777 ( @entity777 ) and matrix metalloproteinases by these cells after treatment with different concentrations of Prev. intermedia lipopolysaccharide (0.2-25 microg/ml). By gelatin zymography, we also characterized the matrix metalloproteinases released by these osteoblasts. Treatment with Prev. intermedia lipopolysaccharide dose-dependently inhibited bone formation by reducing alkaline phosphatase activity and @entity460 incorporation and induced the release of @entity842 , @entity777 and the latent proforms of @entity5780 and @entity1235 by fetal @entity19 osteoblasts in organoid culture. These results indicate that the lipopolysaccharide from Prev. intermedia not only participates in periodontal tissue destruction and alveolar bone resorption, but also inhibits bone formation.
