Transcriptomic analysis of Litchi chinensis pericarp during maturation with a focus on @entity38 degradation and XXXX biosynthesis.

@entity286 @entity3096 @entity38 @entity4211
BACKGROUND: The fruit of litchi (Litchi chinensis) comprises a white translucent edible aril surrounded by a pericarp. The pericarp of litchi has been the focus of studies associated with fruit size, coloration, cracking and shelf life. However, research at the molecular level has been limited by the lack of genomic and transcriptomic information. In this study, an analysis of the transcriptome of litchi pericarp was performed to obtain information regarding the molecular mechanisms underlying the physiological changes in the pericarp, including those leading to fruit surface coloration. RESULTS: Coincident with the rapid break down of @entity38 , but substantial increase of @entity4211 in litchi pericarp as fruit developed, two major physiological changes, degreening and pigmentation were visually apparent. In this study, a cDNA library of litchi pericarp with three different coloration stages was constructed. A total of 4.7 Gb of raw RNA-Seq data was generated and this was then de novo assembled into 51,089 unigenes with a mean length of 737  bp. Approximately 70% of the unigenes (34,705) could be annotated based on public protein databases and, of these, 3,649 genes were significantly differentially expressed between any two coloration stages, while 156 genes were differentially expressed among all three stages. Genes encoding enzymes involved in @entity38 degradation and @entity3096 biosynthesis were identified in the transcriptome dataset. The transcript expression patterns of the Stay Green (SGR) protein suggested a key role in @entity38 degradation in the litchi pericarp, and this conclusion was supported by the result of an assay over-expressing LcSGR protein in @entity286 leaves. We also found that the expression levels of most genes especially late @entity4211 biosynthesis genes were co-ordinated up-regulated coincident with the accumulation of @entity4211 , and that candidate MYB transcription factors that likely regulate @entity3096 biosynthesis were identified. CONCLUSIONS: This study provides a large collection of transcripts and expression profiles associated with litchi fruit maturation processes, including coloration. Since most of the unigenes were annotated, they provide a platform for litchi functional genomic research within this species.
