Downregulation of XXXX receptor by overexpression of phospholipase C-gamma 1 is critical for decrease on focal adhesion in transformed cells.

@entity16626 @entity5301 @entity23687 @entity14 @entity35 @entity3
BACKGROUND: Phospholipase C-yl (PLC-yl) is known to play a critical role in cell adhesion and migration and is highly expressed in @entity3 . In the current study, we found that cells transformed by PLC overexpression (PLC-yl cells) exhibited a marked decrease in expression of the @entity23687 ( @entity23687 ). Here, we assessed the role of @entity23687 -dependent signaling pathways in PLC-yl-dependent regulation of cell adhesion and migration. METHODS: Expression and phosphorylation of @entity23687 and its functional role in PLC-yl cells were evaluated by immunoblot analysis or cell adhesion assay. The mechanism for PLC-y1-induced @entity23687 downregulation was analyzed by blockage of proteosomal degradation with @entity16626 . @entity23687 expression was also confirmed in @entity14 tissues in which PLC-yl was highly expressed. RESULTS: @entity23687 was present on @entity35 fibroblasts, where it functionally active and capable of increasing cell adhesion and migratory activity. However, PLC-yl cells significantly decreased the @entity5301 -dependent effects via ubiquitination-proteosomal degradation of @entity23687 . A marked decrease of @entity23687 expression was confirmed in @entity14 tissues that showed high-level of PLC-yl expression. CONCLUSION: The @entity5301 / @entity23687 complex plays a critical role in the adhesion and migration of @entity35 fibroblasts, and its functional inactivation is associated with PLC-yl-dependent reduction of cell-matrix adhesion and this also affects cell migration.
