Biological activities of a XXXX amniotic membrane interferon.

@entity1 @entity34589 @entity1382 @entity71507 @entity1014 @entity2391 @entity291 @entity3027
In order to characterize further the @entity1 amniotic membrane interferon (IFN-AM), an interferon antigenically unrelated to @entity1 @entity71507 , we analysed its biological activities. Here, we present direct evidence of its ability to affect cell growth and to induce the IFN-stimulated genes (ISGs) 6-16 and 2'-5' @entity1382 ( @entity1382 ), in addition to its crossed anti-viral activity. The cellular growth arrest effect of IFN-AM was dose-dependent and paralleled that of @entity2391 . IFN-AM was also able to inhibit @entity1014 incorporation into DNA, similar to @entity2391 . The mRNA induction of 6-16 gene with IFN-AM treatment reached its highest level at 500 IU/ml and remained constant up to 2000 IU/ml. Conversely, 2'-5' @entity1382 mRNA induction was dose-dependent, with the maximum level detected at 2000 IU/ml of IFN-AM treatment. The time course of mRNA accumulation by ISGs with IFN-AM (500 IU/ml) stimulation was also investigated. Gene induction reached a maximum at 16 h after IFN treatment for 2'-5' @entity1382 and at 48 h for the 6-16 gene. IFN-AM and @entity1 @entity3027 induced similar levels of the @entity1382 enzyme. IFN-AM also showed small but significant activity in @entity291 cells. In conclusion, the amniotic membrane IFN here studied showed both anti-cellular activity and the ability to stimulate ISG-transcriptional activation in a similar manner to @entity2391 . In addition, IFN-AM was also as able to induce the expression of the enzyme 2'-5' @entity1382 , as did @entity3027 . Lastly, amniotic IFN showed a significant cross-species anti-viral activity, which was different from both @entity1 @entity34589 . Taken together, these data strongly suggest that IFN-AM is a novel sub-type I IFN.
