Flow-cytometric identification and follow-up of XXXX exposed to x-irradiation: evaluation of a model system.

@entity19 @entity6732 @entity5
An experimental model system is presented that allows the identification and follow-up of @entity19 exposed to ionizing radiation using flow-cytometric measurements of peripheral blood cells. In an experiment, properties of peripheral blood cells were analysed with flow cytometry for a rapid identification of individuals exposed to radiation. Individuals were then followed longitudinally in an attempt to identify those developing @entity5 . Male CBA @entity19 , 25 days old, were subjected to fractionated x-irradiation (4 x 1.31 Gy) to induce @entity5 . By repeated blood sampling followed by flow cytometry, frequencies of micronucleated erythrocytes and of proliferating nucleated cells were determined. @entity5 were diagnosed by histopathology. Five days after the end of radiation exposure, increased frequencies of proliferating cells, polychromatic erythrocytes and micronucleated normochromatic erythrocytes clearly distinguished the exposed group from the control group. Increased cell proliferation in peripheral blood cells could be used to identify animals with @entity5 , although these animals were at a late stage of @entity5 development. Animals with @entity6732 (not generalized) could not be identified with the flow-cytometric parameters used. We consider that this model system has a potential use when a small number of risk individuals need to be identified and monitored within a large population.
