Genotyping of @entity1 XXXX 3 Isolated from Shanxi Province in China by MLVA16 and HOOF.

@entity1 @entity6475 @entity3377 @entity6467
BACKGROUND: @entity3377 presents a significant economic burden for China because it causes reproductive failure in host species and chronic health problems in @entity1 . These problems can involve multiple organs. @entity3377 is highly endemic in Shanxi Province China. Molecular typing would be very useful to epidemiological surveillance. The purpose of this study was to assess the diversity of @entity6467 strains for epidemiological surveillance. Historical monitoring data suggest that @entity6467 3 is the predominant strain associated with the epidemic of @entity3377 in Shanxi Province. METHODS/PRINCIPAL FINDINGS: Multiple-locus variable-number repeat analysis (MLVA-16) and hypervariable octameric oligonucleotide fingerprinting (HOOF-print) were used to type a @entity1 -hosted @entity6467 population (81 strains). Sixty-two MLVA genotypes (discriminatory index: 0.99) were detected, and they had a genetic similarity coefficient ranging from 84.9% to 100%. Eighty strains of the population belonged to the eastern Mediterranean group with panel 1 genotypes 42 (79 strains) and 43 (1 strain). A new panel 1 genotype was found in this study. It was named 114 MLVAorsay genotype and it showed similarity to the two isolates from Guangdong in a previous study. @entity6467 is distributed throughout Shanxi Province, and like samples from Inner Mongolia, the eastern Mediterranean genotype 42 was the main @entity6475 (97%). The HOOF-printing showed a higher diversity than MLVA-16 with a genetic similarity coefficient ranging from 56.8% to 100%. CONCLUSIONS: According to the MLVA-16 and HOOF-printing results, both methods could be used for the epidemiological surveillance of @entity3377 . A new genotype was found in both Shanxi and Guangdong Provinces. In areas with @entity3377 , the MLVA-16 scheme is very important for tracing cases back to their origins during outbreak investigations. It may facilitate the expansion and eradication of the disease.
