Prevalence of pilus-encoding islets and clonality of pneumococcal isolates from @entity1 with XXXX .

@entity1 @entity21912 @entity6417 @entity697 @entity114 @entity328
We characterized the prevalence of pilus islets 1 ( @entity6417 ) and 2 ( @entity21912 ) and the clonality of @entity328 isolates taken from @entity1 with @entity697 ( @entity697 ) to study the association between pilus existence and @entity697 potential prior to pneumococcal conjugate vaccine and increased antimicrobial resistance. The study material consisted of 75 pneumococcal isolates cultured from the middle ear fluid and/or nasopharyngeal aspirate of 56 @entity1 with @entity697 in Finland during the period 1990-1992. Isolates were studied for antimicrobial susceptibility and were serotyped, genotyped by multilocus sequence typing (MLST), and tested for the presence of pneumococcal @entity6417 and @entity21912 genes. All isolates were susceptible to @entity114 , 14 different serotypes were found, and 20% of the isolates were positive for @entity6417 genes. @entity21912 genes were not found. MLST showed high heterogeneity: 52 @entity697 isolates belonged to 18 known clonal complexes (CC). @entity6417 was associated with serotypes 6A, 6B, and 9V, and genotype CC490. In the time prior to 7-valent pneumococcal conjugate vaccine (PCV7) and increased antimicrobial resistance, @entity697 isolates carried @entity6417 genes at a rather low prevalence. @entity21912 genes were not detected. @entity6417 was related to serotype rather than genotype. The importance of @entity6417 in @entity697 and its association with the spread of antimicrobial resistance requires further research.
