Novel XXXX -Degrading Bacteria Identified by DNA-Stable Isotope Probing.

@entity2556 @entity7352 @entity45299 @entity11966
UNASSIGNED: Microorganisms responsible for the degradation of @entity11966 in a clean forest soil sample were identified by DNA-based stable isotope probing (SIP). The soil was artificially amended with either 12C- or @entity7352 -labeled @entity11966 , and soil DNA was extracted on days 3, 6 and 9. Terminal restriction fragment length polymorphism (TRFLP) results revealed that the fragments of 219- and 241-bp in HaeIII digests were distributed throughout the gradient profile at three different sampling time points, and both fragments were more dominant in the heavy fractions of the samples exposed to the @entity7352 -labeled contaminant. 16S rRNA sequencing of the @entity7352 -enriched fraction suggested that Acidobacterium spp. within the class Acidobacteria, and Collimonas spp. within the class Betaproteobacteria, were directly involved in the uptake and degradation of @entity11966 at different times. To our knowledge, this is the first report that the genus Collimonas has the ability to degrade @entity2556 . Two PAH-RHDa genes were identified in @entity7352 -labeled DNA. However, isolation of pure cultures indicated that strains of Staphylococcus sp. @entity45299 , Pseudomonas sp. PHE-1, and Pseudomonas sp. PHE-2 in the soil had high @entity11966 -degrading ability. This emphasizes the role of a culture-independent method in the functional understanding of microbial communities in situ.
