Effect of inhibin gene immunization on antibody production and reproductive performance in Partridge XXXX hens.

@entity17237 @entity23385
UNASSIGNED: To investigate the effect of inhibin gene immunization on antibody production and reproductive performance in broiler breeder females, Partridge @entity17237 hens aged 380  days were immunized with inhibin recombinant plasmid pcISI. One hundred and twenty hens were randomly assigned to four groups and treated intramuscularly with 25, 75, or 125   g/300- L inhibin recombinant plasmid pcISI (T1   T3) or 300- L saline as control (C), respectively. Booster immunization was given with the same dosage 20  days later. Blood and egg samples were collected to detect the antibody against inhibin by enzyme-linked immunosorbent assay and to evaluate egg performance. The ovaries were collected to classify the follicles and detect the @entity23385 ( @entity23385 ) messenger RNA (mRNA) expression by reverse transcription-PCR. The results showed that immunization against pcISI could elicit antibody against inhibin in both plasma and egg yolk compared with the control (P  <  0.05), whereas booster immunization did not increase the antibody level in plasma. Vaccination promoted egg lay during the first 30  days after primary vaccination (P  <  0.05) with no effect on egg quality and hatching rate. Immunization increased the amounts of dominant, small yellow and large white follicles in the ovary (P  <  0.05). Reverse transcription-PCR results showed that immunization increased the @entity23385 mRNA in the large white follicles, whereas decreased the @entity23385 mRNA in the small yellow follicles (P  <  0.05). In conclusion, inhibin vaccine pcISI can stimulate the production of antibody against inhibin as well as the follicle development and egg laying performance in Partridge @entity17237 hens, which provides a good foundation for the application of inhibin DNA vaccine in avian production.
