Gene expression profiling of laterally spreading XXXX .

@entity5851 @entity4106 @entity5 @entity1836 @entity189 @entity584 @entity14 @entity2270 @entity652 @entity1722
BACKGROUND: Laterally spreading @entity5 (LSTs) are generally defined as lesions >10  mm in diameter, are characterized by lateral expansion along the luminal wall with a low vertical @entity2270 . In contrast to other forms of @entity5 , LSTs are generally considered to have a superficial growth pattern and the potential for @entity5 . We focused on this morphological character of LSTs, and analyzed the gene expression profile of LSTs. METHODS: The expression of 168 genes in 41 @entity14 samples (17 LST- @entity652 , 12 @entity189 , 12 Ip [pedunculated type of the Paris classification)- @entity652 , all of which were 10  mm or more in diameter] was analyzed by PCR array. Based on the results, we investigated the expression levels of genes up-regulated in LST- @entity652 , compared to Ip- @entity652 , by hierarchical and K-means clustering. To confirm the results of the array analysis, using an additional 60 samples (38 LST- @entity652 , 22 Ip- @entity652 ), we determined the localization of the gene product by immunohistochemical staining. RESULT: The expression of 129 genes differed in @entity14 from normal mucosa by PCR array analysis. As a result of K-means clustering, the expression levels of five genes, @entity584 , @entity1836 , @entity1722 , @entity5851 and @entity4106 , were found to be significantly up-regulated (p   <   0.05) in LST- @entity652 , compared to Ip- @entity652 . Immunohistochemical analysis showed that the @entity1836 protein was significantly and meaningfully up-regulated in LST- @entity652 compared to Ip- @entity652 (p   =   0.010). With respect to apoptosis status in LST- @entity652 , it assumes that @entity1836 is anti-apoptotic protein, the samples such as @entity1836 positive and TUNEL negative, or @entity1836 negative and TUNEL positive are consistent with the assumption. 63.2  % LST- @entity652 samples were consistent with the assumption. CONCLUSIONS: LSTs have an unusual profile of gene expression compared to other @entity5 and @entity1836 might be concerned in the organization of LSTs.
