Increased proliferation of a @entity1 @entity0 cell line by recombinant XXXX .

@entity1 @entity0 @entity5 @entity5041 @entity2902 @entity1199 @entity420
Two @entity420 cell lines, Breast M25-SF and Breast M, were established from @entity5 tissue resected surgically from a @entity1 with @entity0 . One, Breast M25-SF, expresses @entity2902 ( @entity2902 ) on the cell surface and the other, Breast M does not. The effects of recombinant @entity1199 ( @entity5041 ) on the proliferation of these cell lines were investigated. The growth of Breast M25-SF was significantly promoted by @entity5041 ranging from 1.25 U/ml to 640 U/ml. Anti- @entity2902 (Tac) antibody significantly blocked the growth enhancement of Breast M25-SF by @entity5041 . Breast M, however, did not respond to @entity5041 . To confirm more directly the promotion of Breast M25-SF growth by @entity5041 , cloning of @entity1199 responders from parent Breast M25-SF cells was carried out by limiting dilution without feeder cells in 96-well microplates. No colony formation was found in 24 wells without @entity5041 . Eleven, 13 and 6 clones were established from groups of 24 wells containing @entity5041 at 200, 20 and 2 U/ml respectively. All of the clones expressed @entity2902 and respond to @entity5041 . By using a sensitive polymerase chain reaction technique, we demonstrated that Breast M25-SF but not Breast M expressed @entity1199 mRNA, and @entity1199 secretion from Breast M25-SF but not Breast M was also confirmed by radioimmunoassay. These findings suggest a role for @entity1199 in autocrine support of Breast M25-SF growth. @entity1199 may play an important role in the growth control of @entity0 cells.
