Multilayered adipose-derived regenerative cell sheets created by a novel magnetite tissue engineering method for XXXX .

@entity3656 @entity10245 @entity63 @entity19 @entity1188 @entity164 @entity741 @entity299 @entity1611 @entity583
BACKGROUND: Adipose-derived regenerative cells (ADRCs) are a promising source of autologous stem cells for regeneration and repair of damaged tissue. Herein, we investigated the therapeutic potential of ADRC sheets created by a magnetite tissue engineering technology (Mag-TE) for @entity583 . METHODS AND RESULTS: Adipose tissue was obtained from @entity164 ( @entity164 ) @entity19 and ADRCs were isolated. ADRCs incubated with magnetic nanoparticle-containing liposomes (MCLs) were cultured. MCL-labeled ADRCs were mixed with a diluted extracellular matrix (ECM) precursor, and a magnet was placed on the reverse side. Magnetized ADRCs formed multilayered cell sheets after a 24-h incubation. @entity164 @entity19 were subjected to @entity583 by permanent ligation of the left anterior descending artery. We then transplanted the constructed ADRC sheet or a cell-free collagen gel sheet, as a control, onto the infarcted myocardium using an Alnico magnet before skin closure. Cardiac parameters were measured by echocardiogram, and angiogenesis was determined by tissue capillary density. ADRC sheet-treated @entity19 showed significant improvements in systolic function, @entity1188 wall thinning, and @entity3656 after @entity583 . ADRC sheet implantation also promoted angiogenesis in both the @entity1188 area and the border zone in @entity164 @entity19 after @entity583 . The angiogenic effects of ADRC sheets were attributed to an increased expression of @entity1611 and @entity10245 mRNA in @entity63 hearts. CONCLUSIONS: ADRC sheets created by this Mag-TE method protect the heart against @entity299 . Our ADRC sheets have the potential to be a novel regenerative strategy for @entity741 .
