Inhibitory effects of traditional Chinese herbal medicine Tanreqing injection on proliferation of XXXX cells in vitro and the potential mechanisms.

@entity981 @entity522 @entity588 @entity2667 @entity4404 @entity2800 @entity1778
OBJECTIVE: The present study investigates the effects of Tanreqing injection, a compound Chinese herbal medicine, on the proliferation of @entity1778 cells in vitro and discusses the potential mechanisms. METHODS: Tanreqing injection was diluted to a series of concentrations (1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, 1:256 and 1:512) by volume and then independently applied to treat chronic @entity2800 K562 cells and T cell @entity981 Molt4 cells at the proliferative stage. Cell growth was observed at different time intervals under a microscope. Cell proliferation was determined by cell counting kit-8 assay and the survival curve was delineated. The inhibitory rate and the half inhibitory concentration (IC50) were calculated. Molt4 cells were stained with @entity2667 (PI) and PI/ @entity4404 and then the cell cycle and apoptosis were analyzed by using flow cytometry. In addition, a real-time quantitative polymerase chain reaction was subjected to detect the expressions of apoptosis-related genes ( @entity588 and @entity522 ) after Tanreqing treatment. RESULTS: Tanreqing injection had inhibitory effects on the proliferation of K562 cells and Molt4 cells. The most toxic concentrations were observed between 1:2 and 1:16 where cells were almost necrotic. The inhibitory effect manifested in a concentration- and time-dependent manner. The IC50 of K562 and Molt4 was 1:333 and 1:142, respectively. After 1:32 Tanreqing injection treatment for 72 h, the number of Molt4 cells in the S phase significantly decreased (P<0.05), and the apoptosis rate markedly increased (P<0.05). In addition, increased @entity522 expression and decreased @entity588 expression were also observed (P<0.05). CONCLUSION: Tanreqing injection can both inhibit the proliferation and promote the apoptosis of @entity1778 cells in vitro, whereby the potential mechanism seems to be mediated in part by decreasing S phase ratio, down-regulating @entity588 expression and up-regulating @entity522 expression.
