Effects of candidate autocrine and paracrine mediators on growth responses in isolated XXXX arteries.

@entity664 @entity1808 @entity2918 @entity534 @entity2135 @entity1827 @entity842 @entity35 @entity3352
We evaluated the effects of mediators that can be produced by smooth muscle and endothelial cells on growth responses in isolated arteries. Segments of carotid and renal arteries, denuded of endothelium, were isolated from adult @entity35 and studied during tissue culture in the presence of @entity3352 . Three days of culture in the presence of serum stimulated DNA synthesis in the media. During long-term culture new layers of cells developed at the borders of the arterial segments. Medial DNA synthesis depended less on serum than extramedial cell proliferation. During moderate stimulation, @entity664 and @entity1827 enhanced and interleukin-1 and transforming growth factor-beta reduced medial DNA synthesis, whereas @entity2135 , platelet-derived growth factor AA, platelet-derived growth factor BB, and @entity1808 were without effect. Of these factors, only @entity1827 stimulated extramedial cell proliferation. In addition, serum-stimulated but not @entity664 -stimulated medial DNA synthesis was less marked in arteries that had not been denuded of endothelium than in ee-endothelialized arteries. Differences between preparations with and without endothelium persisted in the absence of @entity534 and in the presence of an inhibitor of @entity842 synthase. These observations confirmed that DNA synthesis in the arterial media and extramedial cell proliferation are influenced by different factors. They further indicated that endothelial modulation of medial DNA synthesis does not seem to involved endothelium-derived @entity2918 , @entity842 , or interleukin-1 and that it can be blunted by @entity664 .
