3-[4-(3-Trifluoromethyl-phenyl)-piperazin-1-yl]-dihydrofuran-2-one and pregabalin attenuate tactile @entity1931 in the @entity19 model of XXXX .

@entity1931 @entity19 @entity7215 @entity51 @entity8638 @entity9
PURPOSE: There is a strong medical demand to search for novel, more efficacious and safer than available, analgesics for the treatment of @entity9 . This study investigated antinociceptive activity of intraperitoneally administered 3-[4-(3-trifluoromethyl-phenyl)-piperazin-1-yl]-dihydrofuran-2-one ( @entity7215 ) and pregabalin in the @entity8638 ( @entity8638 ) model of @entity9 in @entity19 and evaluated these drugs' influence on motor coordination. In addition, microscopic examinations of the sciatic nerve were performed to assess, if a surgical method or drug treatment caused changes in the structure of this nerve. Moreover, the alterations of nerve growth factor (NGF) content after drug treatment were assessed. METHODS: Antiallodynic and antihyperalgesic activities of @entity7215 and pregabalin were assessed in the von Frey and hot plate tests. Motor-impairing properties were evaluated in the rotarod test. Microscopic examinations of the sciatic nerve were performed using electron microscope. In immunohistochemical assays the content of NGF in the sciatic nerve after single or repeated administration of test drugs was assessed. RESULTS: Microscopic examinations of the sciatic nerve revealed ultrastructural changes in nerve fibers indicating for neurodegenerative processes induced by @entity8638 . Seven days after @entity8638 surgery @entity7215 and pregabalin reduced tactile @entity1931 in von Frey test (ED50 values were 1.5 and 15.4   mg/kg, respectively). None of the test drugs at dose range 0.5-100   mg/kg induced @entity51 in the rotarod test. In immunohistochemical assays repeated doses of pregabalin and @entity7215 elevated NGF content. CONCLUSIONS: @entity7215 has antiallodynic properties and is an interesting lead structure in the search for novel analgesics used in @entity9 .
