@entity3088 inhibit XXXX / @entity540 -induced angiogenesis in vitro by inhibiting the matrix-degrading proteases.

@entity1 @entity1552 @entity3334 @entity2289 @entity670 @entity5 @entity1476 @entity7 @entity1551 @entity3088 @entity15476 @entity21538 @entity23625 @entity540 @entity2843 @entity563 @entity16617 @entity2290
@entity3088 have been proposed to act as chemopreventive agents in numerous epidemiological studies and have been shown to inhibit angiogenesis and proliferation of @entity5 cells and endothelial cells in vitro. Angiogenesis requires tightly controlled extracellular matrix degradation mediated by extracellular proteolytic enzymes including matrix metalloproteinases (MMPs) and @entity563 proteases, in particular, the urokinase-type plasminogen activator ( @entity2290 )-plasmin system. In this study, we have investigated the antiangiogenic mechanism of the @entity3088 , @entity670 , @entity15476 , and @entity21538 in a @entity1 umbilical vein endothelial cell (HUVEC) model. The stimulation of serum-starved HUVECs with vascular endothelial growth factor/basic fibroblast growth factor ( @entity23625 ) caused marked increase in @entity3334 production and induced the pro- @entity1476 activation accompanied by the increase in @entity1551 expression. However, pretreatment with @entity3088 before @entity7 / @entity540 stimulation completely abolished the @entity7 / @entity540 -stimulated increase in @entity3334 and @entity1551 expression and pro- @entity1476 activation. @entity670 blocked @entity7 / @entity540 -stimulated increase in @entity2843 expression and decrease in @entity1552 expression. @entity15476 and @entity21538 further decreased @entity2843 expression below basal level and completely abolished @entity1552 expression. @entity7 and @entity540 stimulation also significantly induced @entity2290 expression, most strikingly the level of 33 kDa @entity2290 , and increased the expression of @entity2289 . @entity670 , @entity15476 , and @entity21538 effectively blocked the generation of 33 kDa @entity2290 , and further decreased the activity of the 55 kDa @entity2290 and the expression of @entity2289 below the basal level. In conclusion, these data suggest that @entity670 , @entity15476 , and @entity21538 inhibit in vitro angiogenesis, in part via preventing @entity7 / @entity540 -induced @entity3334 and @entity2290 expression and the activation of pro- @entity1476 , and via modulating their inhibitors, @entity16617 , and @entity2289 .
