[Expression of @entity5855 , vasoactive intestinal peptide and XXXX in the mucosa of the neovagina after sigmoid colon vaginoplasty].

@entity1 @entity3549 @entity7766 @entity5855 @entity121
OBJECTIVE: To investigate re-innervation in the neovaginal mucosa of @entity1 underwent sigmoid colon vaginoplasty in treatment of Mayer-Rokitansky-Kistner-Hauser Syndrome (MRKHS). METHODS: Biopsies in the upper third of the posterior neovagina were taken in 20 @entity1 treated by sigmoid colon vaginoplasty at 1, 2 and 3 years after surgery, respectively. @entity5855 ( @entity5855 ), vasoactive intestinal peptide ( @entity3549 ) and @entity7766 ( @entity7766 ) were detected by immunohistochemical method and compared with those in intact sigmoid colon mucosa. RESULTS: (1) Density of nerve fiber: abundant distribution of @entity5855 nerve fibers were observed in the mucosal @entity121 , submucosa, and smooth @entity121 of the neovagina. The nerve fibers of @entity3549 and @entity7766 immunoreactivity were mainly distributed around blood vessels and in the smooth muscles. In the neovagina, the density of nerve fibers of @entity5855 of 17    6 were much more than @entity3549 of 2.9    1.0 and @entity7766 of 2.5    0.8 significantly (P < 0.05). (2) Expression of @entity5855 in neovagina: at 1 year after surgery, @entity5855 positive expression of 14    4 was significantly lower in the neovagina than 28    7 in the intact sigmoid colon (P < 0.05). However, after 2 to 3 years, its expression displayed an upgrade tendency in the neovagina and was significantly higher at the 3 year postoperatively than that at the 1 years postoperatively (22    7 vs. 14    4, P < 0.05). The changes were much more obvious in submucosa. (3) The expression of @entity3549 and @entity7766 in neovagina: at 1 year after surgery, @entity3549 and @entity7766 positive nerve fibers were also decreased in the neovagina when compared with those in the intact sigmoid colon (2.3    0.7 vs. 5.3    1.4, P < 0.05; 2.5    1.1 vs. 5.5    1.1, P < 0.05). At 2 to 3 years after surgery, the positive @entity3549 fiber showed initially decreased and subsequently increased tendency. The density of @entity3549 of 3.7    0.7 in the neovagina at 3 years postoperatively was higher than 2.3    0.7 at 1 years postoperatively (P < 0.05). No significant up-regulation was observed in @entity7766 -positive expression in the neovagina within 3 years after operation. CONCLUSIONS: Distribution of sensory @entity5855 , @entity3549 and @entity7766 immunoreactive nerve fibers was similar to the pattern observed within the intact sigmoid colon wall. The number of nerve fibers in the neovagina decreased after surgery and then increased subsequently within 3 years after surgery.
