Assessment of the biological properties of XXXX split skin allografts disinfected with @entity3879 and preserved in @entity653 .

@entity1 @entity2771 @entity137 @entity310 @entity1066 @entity653 @entity3880 @entity1391 @entity3879 @entity130
Skin allografts derived from cadaveric @entity1 donors are widely used in the treatment of serious @entity130 and other conditions, such as @entity1066 . In order to render these allografts safe for clinical use, and to enable them to be preserved and banked for long periods, effective methods of decontamination and preservation are required. These methods must not adversely affect graft properties essential for clinical performance. We have investigated the application of a @entity3879 ( @entity3880 ) disinfection protocol, coupled with preservation in either @entity653 or @entity2771 to achieve these goals. An effective decontamination procedure, comprising of a 3h exposure to 0.1% (v/v) @entity3880 in @entity1391 buffered saline (PBS) at pH 7.0, was developed and had no significant detrimental effects on the structure of skin. Cadaveric skin allografts were then treated with this disinfection protocol and subsequently preserved in either 85% (v/v) @entity653 or @entity2771 in PBS, and the biological properties of the allografts thought to be essential to successful clinical performance were assessed. The @entity137 of the grafts was assessed using both extract and contact assays; damage to the skin collagen was assessed using a collagenase susceptibility assay and the capacity of the grafts to elicit an inflammatory response in vitro was assessed by quantifying the production of the pro-inflammatory cytokine @entity310 by @entity1 peripheral blood mononuclear phagocytes. Neither the disinfection protocol nor either of the preservation techniques rendered the grafts cytotoxic or pro-inflammatory. The @entity3880 disinfection and @entity653 preservation protocol had no effects on collagenase susceptibility, whereas the disinfection protocol in combination with @entity2771 rendered some of the test samples significantly more susceptible to collagenase digestion. Therefore, this study has demonstrated that @entity3880 disinfection combined with @entity653 preservation is suitable for skin allografts. The use of @entity2771 as a preservation agent for skin requires further development.
