Screening for XXXX target genes in colorectal cancers.

@entity124 @entity70999 @entity5 @entity5345 @entity996 @entity26994 @entity694
BACKGROUND: Defects in the DNA repair system lead to genetic instability because replication errors are not corrected. This type of genetic instability is a key event in the malignant progression of HNPCC and a subset of sporadic @entity694 and mutation rates are particularly high at short repetitive sequences. Somatic deletions of coding mononucleotide repeats have been detected, for example, in the TGFbetaRII and BAX genes, and recently many novel target genes for @entity124 ( @entity124 ) have been proposed. Novel target genes are likely to be discovered in the future. More data should be created on background mutation rates in @entity5 to evaluate mutation rates observed in the candidate target genes. METHODS: Mutation rates in 14 neutral intronic repeats were evaluated in @entity5 . Bioinformatic searches combined with keywords related to @entity5 and @entity5 suppressor or CRC related gene homology were used to find new candidate @entity124 target genes. By comparison of mutation frequencies observed in intronic mononucleotide repeats versus exonic coding repeats of potential @entity124 target genes, the significance of the exonic mutations was estimated. RESULTS: As expected, the length of an intronic mononucleotide repeat correlated positively with the number of slippages for both G/C and A/T repeats (p=0.0020 and p=0.0012, respectively). @entity5345 , @entity70999 , and @entity996 associated @entity26994 and other candidates were found in a bioinformatic search combined with keywords related to @entity5 . Sequencing showed a significantly increased mutation rate in the exonic A9 repeat of @entity26994 (25/109=22.9%) as compared with similar intronic repeats (p< or =0.001). CONCLUSIONS: We propose a new candidate @entity124 target gene @entity26994 to be evaluated in further studies.
