Flow cytometry of XXXX : relationship of DNA content to survival.

@entity1 @entity2007 @entity263 @entity5 @entity982
Between March 1970 and December 1978 there were 366 @entity1 with @entity263 treated by 125I seed implants and pelvic lymph node dissection. All had a minimum of 5 years follow-up. One hundred thirty-three @entity1 had metastatic @entity263 in lymph nodes (Stage D1) at the time of lymph node dissection and seed implantation. Ninety-one of the 133 @entity1 were judged to have sufficient metastatic @entity263 in their nodal tissue (greater than 50% replacement with @entity5 ) to justify flow cytometric cellular DNA measurements on the involved @entity982 -embedded nodal tissue. Nine @entity1 were excluded due to uninterpretable DNA histograms leaving 82 @entity1 for analysis. Forty-nine @entity1 had @entity2007 and 33 had @entity5 . There was no statistical bias between the @entity2007 and diploid groups due to age (P = 0.970, chi 2 test), time between diagnosis and implantation (P = 0.217, chi 2 test), number of positive nodes (P = 0.669, two-sample t test of means), or @entity5 grade (P = 0.332, chi 2 test). Median survival time of the @entity2007 and diploid groups was 5.0 and 8.8 years, respectively (P = 0.0109, log rank test). Cox regression analysis confirmed the effect of aneuploidy versus diploidy on survival by controlling for other potentially confounding variables (age, time from diagnosis to implantation, number of positive nodes, and grade). Grade as a predictor of survival did not approach statistical significance in this series of relatively small size (P = 0.116). Thirty-eight of the 82 @entity1 had moderately differentiated @entity5 . Nineteen of these were @entity2007 and 19 diploid. The median survival was 5.8 and 9.1 years, respectively, for these grade-matched @entity2007 and diploid groups (P = 0.039, log rank test). We conclude that flow cytometric DNA measurements on archived paraffin-embedded @entity5 in nodal metastases appear to be a strong predictor of survival for Stage @entity263 .
