@entity8561 Transport and XXXX Production in Kinin Receptor B1-/- Endothelial Cells.

@entity8561 @entity19 @entity71161 @entity8816 @entity534 @entity842 @entity164 @entity3689 @entity17462 @entity934
UNASSIGNED: Kinins are important vasoactive peptides, but the role of the B1 receptor subtype in the vascular control is poorly understood. This study analyzed the @entity842 (NO) release, @entity8561 ( @entity534 ) uptake and the expression of the cationic @entity3689 transporter (CAT) -1 in endothelial cells obtained from B1 receptor knockout (B1-/-) and @entity164 ( @entity164 ) @entity19 . NO production was assessed through a fluorescent dye in living cells stimulated with @entity934 . @entity534 uptake was determined indirectly in the culture medium by HPLC, in the presence or absence of the @entity71161 blocker @entity8816 ( @entity17462 ). @entity71161 mRNA levels and protein expression were determined by qPCR and western blot, respectively. NO release was significantly reduced in B1-/- when compared to @entity164 cells. This result was accompanied by a decreased rate in the @entity534 uptake by B1-/- cells. Incubation with @entity17462 impaired the @entity534 uptake in @entity164 , but had no effect in B1-/- cells. Protein expression and mRNA levels for @entity71161 were reduced in B1-/- in comparison to @entity164 cells. These findings suggest an important role of the endothelial B1 receptor in the vascular control by interfering with @entity71161 expression, @entity534 uptake and NO release.
