Induction of megakaryocytic differentiation in a newly established @entity1 @entity1778 cell line by XXXX .

@entity1 @entity1778 @entity10706 @entity865 @entity1427 @entity1428 @entity27463 @entity864 @entity1968
BACKGROUND: Biological studies on megakaryopoiesis have been hampered by the scarcity of megakaryocytes in normal bone marrow and difficulty in long term culture. Alternatively, @entity1778 cell lines with megakaryocytic differentiation potential may provide good models to counter these problems. METHODS: Leukemic cells from a @entity1 with @entity10706 were put into long-term culture and established into a cell line which was designated as VGH-MK1. The VGH-MK1 cells were challenged with differentiation agents and/or cytokines, and the differentiation of these cells was examined using morphological, immunocytochemical and surface-marker studies. RESULTS: Morphologically, VGH-MK1 cells had prominent nucleoli and basophilic cytoplasm with some protrusions, but large cells were occasionally seen. Under regular culture condition, the cells had a doubling time of 36-48 hours. The cloned cell line exhibited markers characteristic of megakaryoblasts after differentiation induction. Specifically, when stimulated with @entity864 ( @entity865 ), cells became larger and had large or multinuclei. They were induced to express platelet glycoproteins GPIb ( @entity27463 ), @entity1427 /IIIa ( @entity1427 ), and @entity1428 ( @entity1428 ) antigens, but not erythroid nor lymphoid markers. Platelet peroxidase (PPO) activity was also induced. @entity1968 did not exhibit similar differentiation-inducing effects. In contrast, it counteracted the effects induced by @entity865 . CONCLUSIONS: An unique @entity1 @entity1778 cell line, VGH-MK1, has been established here. It could be induced to exhibit some characteristics of megakaryocytic lineage, and may be an useful model for the biological studies of megakaryopoiesis.
