Immunosuppressive substances in XXXX culture filtrate.

@entity1 @entity20403 @entity19 @entity54 @entity3 @entity204
@entity3 has become a serious problem in clinical practice, but the actual factor that confers virulence on the fungus has not been thoroughly elucidated. To identify and isolate the immunosuppressive substances produced by the fungus, the bioactivity of culture filtrates was assessed, and analyses of the culture filtrates were carried out. Culture filtrates from different strains of @entity54 were assessed for their effect on @entity1 polymorphonuclear leukocytes and @entity19 macrophages. To assess their activities in vivo, their effect on the survival of @entity19 infected by the fungus was also studied. Subsequently, the composition of the culture filtrates was analyzed by gas chromatography-mass spectrometry. The analyses revealed that the culture filtrates contained @entity20403 at concentrations of 3 to 4 microgram/ml, and some other unidentified compounds. The bioactivities of the culture filtrates were similar to those of @entity20403 . The fungal culture filtrate reduced the survival of infected @entity19 , but the filtrate itself did not cause the @entity204 of @entity19 . However, all the bioactivities could not be accounted for by @entity20403 itself. These results indicate that @entity20403 in the culture filtrates may be responsible for part of the immunosuppressive activity, but some other components produced by A. fumigatus contribute, in an additive or synergistic manner, to the virulence of the fungus.
