mRNA and protein expression of XXXX , @entity36030 and their receptors in the porcine umbilical cord during pregnancy.

@entity1707 @entity59222 @entity72994 @entity72993 @entity30785 @entity36030
The fibroblast growth factors (FGFs) are multifunctional proteins that, among other roles, regulate structural reorganization of uterine and placental vascular bed during pregnancy. Thus, we analyzed mRNA and protein expression and immunohistochemical localization of @entity72993 and @entity36030 , and their receptors ( @entity59222 and @entity30785 ) in the developing umbilical cord (UC) on days 40, 60, 75 and 90 of pregnancy and after the physiological delivery in the @entity1707 (day 114). qPCR analysis demonstrated an increase in @entity72993 and @entity36030 mRNA levels beginning on day 75 and on day 114 of pregnancy, respectively. In addition, significantly increased @entity72994 mRNA expression was also found on day 114. On the other hand, no significant changes in @entity30785 mRNA expression were observed. Western Blot analysis revealed a decrease in @entity72993 and @entity30785 protein expression after day 40. Beside an increased protein expression of @entity36030 on day 60, no significant changes in @entity59222 protein expression were detected. Immunohistochemical staining enabled detection of FGF- @entity30785 system, with different intensity of immunoreaction in endothelial and tunica media cells of the umbilical vessels and in allantoic duct and amniotic epithelium as well as in myofibroblasts. In conclusion, our results show that members of FGF- @entity30785 system are expressed specifically in UC structures. Furthermore their day of pregnancy-related expression suggest that they may be an important players during UC formation and development.
