Characterization of XXXX gene mutations in @entity14 from an Asian population.

@entity5 @entity3 @entity876 @entity14
A series of @entity14 from an Asian population in Singapore were analyzed for mutations in the @entity5 suppressor gene @entity876 . Based on single-strand conformation polymorphism (PCR-SSCP) analysis and direct DNA sequencing, 15 of 38 @entity5 (39%) were found to contain mutations in exons 5-8 of the @entity876 gene. The point mutations were predominantly base transitions. Among the 10 transitions, 5 were at CpG dinucleotide sites. One-third (5/15) of the mutations were found at previously identified hotspot codons 175, 248 and 282. In one case, an insertion of a 6-base pair sequence was found. @entity876 mutations did not correlate with @entity5 histological grade, @entity5 , or @entity3 . However, @entity5 at the distal site showed a higher proportion of mutations than the proximal site. Further, no mutation was found in the normal mucosa adjacent to @entity5 site, suggesting that no germ-line mutations were present. The results were compared with those from other studies and are discussed in connection to possible etiological factors that are specific to the local population.
