Adenovirus-mediated ICOSIg gene transfer alleviates cardiac remodeling in experimental XXXX .

@entity296 @entity6930 @entity72736 @entity11017 @entity11018 @entity41831 @entity2834 @entity6929 @entity299 @entity35 @entity46894 @entity2401
To explore the therapeutic effects of adenovirus vector mediated transfer of the ICOSIg gene on immuno-inflammation-mediated cardiac remodeling in an experimental @entity2401 (EAM) model, pAdeno-ICOSIg was constructed and transfected into HEK 293 cells to produce the ICOSIg adenovirus. Ad- @entity2834 -GFP was used as a control. EAM was induced in Lewis @entity35 by injection of porcine cardiac myosin. The immunized @entity35 were divided into two groups. The inducible co-stimulatory molecule ( @entity41831 ) group received the adenovirus containing ICOSIg on day 14; the green fluorescent protein (GFP) group received the adenovirus containing GFP as the control adenovirus and 15 normal @entity35 (Control group) consisted of the normal controls that were not immunized. On day 28, all @entity35 were euthanized after echocardiography and histopathologically examined for @entity299 . Western blotting was performed to detect @entity41831 , @entity46894 ( @entity46894 ), @entity11017 , @entity6929 , @entity6930 and @entity11018 and real-time RT-PCR was performed to detect B7-1, B7-2 and interleukin (IL)-17 expression. ELISPOT was applied to detect Th1 and Th2 cytokine production. Collagen concentration and collagen cross-linking were determined as markers of @entity299 . It was found that blockade with ICOSIg exerted antifibrotic effects on cardiac remodeling in EAM. On day 28, cardiac function and inflammatory @entity296 improved significantly in the @entity41831 group compared to the GFP group. The expression of @entity41831 , the @entity46894 , @entity72736 was statistically significantly lower in the @entity41831 and Control groups compared to the GFP group. ICOSIg significantly augmented Th2 cytokine production and diminished Th1 and Th17 cytokine production. This blockade of the @entity41831 co-stimulatory pathway with ICOSIg alleviated autoimmune inflammation-mediated cardiac remodeling and improved cardiac function. Regulation of the Th1/Th2/Th17 balance may be one of the underlying mechanisms responsible for this effect.
