Expression of sirtuin 1 and 2 is associated with poor prognosis in XXXX @entity1 .

@entity1 @entity2125 @entity4093 @entity5 @entity957 @entity2 @entity1149 @entity11935 @entity420
BACKGROUND: @entity4093 ( @entity4093 ) and @entity11935 ( @entity11935 ) are @entity2125 -dependent protein deacetylases involved in the regulation of key @entity5 -associated genes. In this study we evaluated the relevance of these deacetylases in @entity2 biology. MATERIAL AND METHODS: Protein levels of @entity4093 and @entity11935 were determined in @entity1149 ( @entity1149 ) cell lines and @entity5 from 105 @entity1 . Changes in proliferation were assessed after @entity4093 and @entity11935 downregulation in @entity2 cell lines using siRNA-mediated technology or tenovin-1, a @entity4093 and @entity11935 inhibitor. RESULTS: High @entity4093 and @entity11935 protein levels were found in @entity1149 cell lines compared with non-tumor lung epithelial cells. The expression of @entity4093 and @entity11935 proteins was also significantly higher in lung @entity5 than in normal tissue (P<0.001 for both sirtuins). Stronger nuclear @entity4093 staining was observed in @entity420 than in @entity957 (P=0.033). Interestingly, in @entity1149 @entity1 , high @entity4093 and @entity11935 expression levels were associated with shorter recurrence-free survival (P=0.04 and P=0.007, respectively). Moreover, the combination of high @entity4093 and @entity11935 expression was an independent prognostic factor for shorter recurrence-free survival (P=0.002) and overall survival (P=0.022). In vitro studies showed that @entity4093 and/or @entity11935 downregulation significantly decreased proliferation of @entity1149 . CONCLUSIONS: Our results support the hypothesis that @entity4093 and @entity11935 have a protumorigenic role in @entity2 , promoting cell proliferation. Moreover, the expression of these proteins is associated with poor prognosis in @entity1149 @entity1 and may help to identify those @entity1149 @entity1 with high risk of recurrence that could benefit from adjuvant therapy after resection.
