Development of a monoclonal antibody to the XXXX 8.5S uterine @entity1871 receptor.

@entity172 @entity0 @entity4859 @entity19 @entity5 @entity840 @entity95 @entity692 @entity1871 @entity1582 @entity969 @entity1573 @entity8851 @entity35
Nonactivated (8.5S) @entity95 uterine @entity1871 receptor was enriched 10- to 30-fold by chromatography on columns of spheroidal @entity692 and @entity8851 -cellulose. A total of approximately 25 micrograms of receptor (purity approximately 1%) was injected at multiple sites into a BALB/c @entity19 . After several injections, splenic lymphocytes were fused with P3x63Ag8.653 @entity19 @entity1582 cells. This fusion produced in excess of 240 hybridomas, which were screened by an enzyme-linked immunosorbent assay (ELISA), solid-phase radioimmunoassay, and @entity969 gradient centrifugation. One colony (KN 382/EC1) produced a @entity19 immunoglobulin G1 which bound @entity95 8.5S uterine @entity1871 receptor. The cell line has been repeatedly cloned under conditions of limiting dilution and expanded in @entity19 as @entity5 . Antibody was purified by @entity4859 precipitation, @entity8851 -cellulose chromatography, and affinity chromatography with protein A - Sepharose CL-4B. Specificity of the antibody was determined by @entity969 gradient centrifugation and solid-phase radioimmunoassay. The antibody bound to @entity1871 receptors from @entity95 uterus and MCF-7 @entity0 cells. It did not react with @entity1871 receptors from @entity35 uterus, @entity840 uterus, or @entity1573 oviduct, nor did it bind to @entity172 receptors from any of the tissues we tested.
