XXXX Administration After Sublethal Lower Limb @entity63 Reduces Inflammatory Reaction and Offers Organ Protection in @entity63 /Reperfusion Injury.

@entity3264 @entity63 @entity19 @entity651 @entity7647 @entity777
BACKGROUND: This study investigated the effects of intravenous @entity3264 ( @entity7647 ) administration on the expression of adhesion molecules and inflammatory mediators in a @entity19 model of hind limb @entity63 /reperfusion (IR) injury. METHODS: There were 3 IR groups and 1 normal control (NC) group. The NC group did not undergo the IR procedure. @entity19 in the IR groups underwent 90 minutes of limb @entity63 followed by a variable period of reperfusion. @entity63 was performed by applying a 4.5-oz orthodontic rubber band to the left thigh. @entity19 in one IR group were sacrificed immediately after reperfusion. The other 2 IR groups were injected once with either 0.75 g @entity7647 /kg body weight (G group) or an equal volume of saline (S group) via tail vein before reperfusion. @entity19 in the S and G groups were subdivided and sacrificed at 4 or 24 hours after reperfusion. RESULTS: IR enhanced the inflammatory cytokine gene expressions in muscle. Also, plasma @entity777 levels, blood neutrophil percentage, and the adhesion molecule and chemokine receptors expressed by leukocytes were upregulated after reperfusion. The IR-induced muscle inflammatory mediator gene expressions, blood macrophage percentage, and plasma @entity777 concentration had declined at an early or a late phase of reperfusion when @entity7647 was administered. Histologic findings also found that @entity651 was attenuated during IR insult. CONCLUSIONS: A single dose of @entity7647 administration immediately after sublethal lower limb @entity63 reduces the inflammatory reaction locally and systemically; this may offer local and distant organ protection in hind limb IR injury.
