MicroRNA-96 plays an oncogenic role by targeting @entity4012 and regulating XXXX / @entity4012 / @entity22030 pathway in @entity3557 cells.

@entity3557 @entity584 @entity4012 @entity22030 @entity15293 @entity26365
MicroRNAs (miRNAs) are kind of small non-coding RNAs that negatively regulate gene expression at post-transcription level, and those non-coding RNAs appear to play a key role in tumorigenesis. The aim of this study was to investigate the biological role of @entity26365 in @entity3557 ( @entity3557 ) cell lines. We identified @entity26365 to be up-regulated in @entity3557 specimens in comparison to matched normal tissues by microRNA microarray and RT-qPCR analysis (P < 0.05). Next, to explore the potential function of @entity26365 , @entity3557 cell lines K1 and @entity15293 were transiently transfected with @entity26365 mimics and inhibitor. Successful transfection being confirmed by RT-qPCR. Ectopic expression of @entity26365 promoted proliferation and colony formation ability, and inhibited apoptosis of K1 and @entity15293 cells, whereas down-regulated expression of @entity26365 suppressed those functions when compared with the control cells. According to a computational prediction, @entity4012 maybe a potential target of @entity26365 . Luciferase assays revealed that @entity26365 is directly targeted to both binding sites of @entity4012 3'-untranslated region (3'-UTR) and suppressed the @entity4012 expression, and subsequently inhibited the expression of @entity22030 protein in @entity3557 cells. Moreover, the expression of @entity4012 had an inverse correlation with expression of @entity26365 in @entity3557 specimens by RT-qPCR and western blot analysis. The data from the present study demonstrated that @entity26365 can promote proliferation, and inhibit apoptosis in @entity3557 cell lines K1 and @entity15293 , thus @entity26365 may play an oncogenic role in @entity3557 by inhibiting the @entity4012 and regulating @entity584 / @entity4012 / @entity22030 pathway, and it may serve as a novel therapeutic target for miRNA-based @entity3557 therapy.
