Reactions of granular pericytes in a XXXX cerebrovascular ischemia model.

@entity1 @entity21170 @entity3584 @entity406 @entity95 @entity1710 @entity1856
This study was undertaken to examine some of the quantitative and qualitative changes which might occur in the cerebral granular pericyte population when comparing control and embolically stressed animals. Eight animals, male NZW 2-2.5 kg @entity95 , were given intracarotid sublethal injections of @entity1 @entity1856 concentrated at 125 mg/ml. Eight others received 1 cc of sterile saline. Two hours after injection the brains were fixed with either neutral buffered @entity406 or 3% buffered @entity3584 by cardiac perfusion at 110 mmHg pressure. The brains were removed and sliced, anteroposteriorly, in 3 mm slices. Each slice from five brains for each condition was then processed for paraffin sectioning and staining with @entity1710 and PAS. Two animals for each condition underwent the same treatments and were processed for frozen sectioning and staining with @entity21170 . One animal for each condition had each brain slice sectioned by vibratome (40 micrometers) and was processed appropriately and examined by either fluorescence microscopy, standard electron microscopy or acid phosphatase electron microscopy. In the groups stained with @entity1710 and PAS, granular pericytes were counted for each of the first five levels of each brain for both the control and experimental conditions. The results of this study reconfirmed that these cells were granular pericytes; that is, they were autofluorescent and PAS positive; were surrounded by a basement membrane, were acid phosphatase positive and contained a heterogenous granular cytoplasmic inclusion population. Further, after ischemic insult, the number of granular pericytes increased significantly at two hours. It was also shown that these cells appeared capable of accumulating lipid components of the injected @entity1856 from the vessel lumen.
