Alterations in mucosal content of colonic glycoconjugates in XXXX defined by monoclonal antibodies.

@entity1 @entity32 @entity31 @entity29 @entity694 @entity30
The presence of several glycoconjugates in colonic mucosa of @entity1 with @entity29 was assessed through indirect immunofluorescent staining using a collection of 23 monoclonal antibodies directed against @entity1 @entity694 (anti-HCM MAbs). Intensity and distribution of staining by three anti-HCM MAbs were significantly altered in mucosa from @entity1 with @entity31 ( @entity31 ) (n = 14) when compared with normal tissue (n = 15) and with tissue from @entity1 with @entity30 as well as other @entity32 (n = 15). Staining by anti-HCM MAb 17, which binds to colonic mucin glycoprotein species IV and V, was absent or diminished in 86% of samples from @entity1 with active @entity31 in contrast to 14% of normal and disease control specimens. Reduction in anti-HCM MAb 17 staining was less marked in mucosal biopsy specimens from @entity1 with @entity31 lacking acute inflammatory activity (n = 8). In contrast to the apparent loss of the MAb 17-defined epitopes, staining with anti-HCM MAbs 10 and 22 was enhanced in @entity31 tissue compared with normal and disease controls. Increased staining with MAb 10 was present in 93% of samples from @entity31 @entity1 demonstrating active @entity32 . Increased MAb 10 staining was not apparent in noninvolved mucosa from @entity31 @entity1 , indicating that increased expression of the specified epitope is related to the acute inflammatory process. In contrast, indirect immunofluorescent staining with MAb 22 was apparent in both involved (78%) and uninvolved (67%) @entity31 mucosa in contrast to normal and disease controls (less than 12%). In addition, staining with several other anti-HCM MAbs (MAbs 3, 11, 15) was modestly and variably diminished (14%-28%) in @entity31 , @entity30 , and other @entity32 . These findings demonstrate the presence of alterations in mucosal content of specific glycoconjugate structures in association with @entity31 . Inflammatory processes may also result in broad changes in glycoconjugate determinants generally.
