Let-7a regulates mammosphere formation capacity through Ras/NF-kB and Ras/MAPK/ @entity3963 pathway in XXXX stem cells.

@entity5 @entity984 @entity0 @entity3963
UNASSIGNED: @entity0 stem cells (BCSCs) have the greatest potential to maintain tumorigenesis in all subtypes of @entity5 cells and were regarded as the key drivers of @entity5 . Recent evidence has demonstrated that BCSCs contributed to a high degree of resistance to therapy. However, how BCSCs self renewal and tumorigenicity are maintained remains obscure. Herein, our study illustrated that overexpression of let-7a reduced cell proliferation and mammosphere formation ability of @entity0 stem cells(BCSCs) in a @entity984 -dependent manner through different pathways in vitro and in vivo. To be specific, we provided the evidence that let-7a was decreased, and reversely the expression of @entity984 was increased with moderate expression in early stages (I/II) and high expression in advanced stages (III/IV) in @entity0 specimens. In addition, the negative correlation between let-7a and @entity984 was clearly observed. In vitro, we found that let-7a inhibited mammosphere-forming efficiency and the mammosphere-size via NF-kB and MAPK/ @entity3963 pathway. The inhibitory effect of let-7a on mammosphere formation efficiency and the size of mammospheres was abolished after the depletion of @entity984 . On the contrary, enforced expression of @entity984 rescued the effect of let-7a. In vivo, let-7a inhibited the growth of @entity5 , whereas the negative effect of let-7a was rescued after overexpressing @entity984 . Taken together, our findings suggest that let-7a played a @entity5 suppressive role in a @entity984 -dependent manner.
