Treatment with bioscaffold enhances the the fibril morphology and the collagen composition of healing medial collateral ligament in XXXX .

@entity10036 @entity95 @entity214
Porcine small intestinal submucosa (SIS) was shown to be an effective bioscaffold in enhancing the mechanical properties of healing @entity10036 ( @entity10036 ). The purpose of this study was to investigate whether there are corresponding improvements in morphology and tissue compositions. Fourteen @entity95 were equally divided into two groups. In the SIS-treated group, a 6 mm gap was surgically created in the right @entity10036 and a layer of SIS was sutured covering the gap. For the nontreated group, the gap-injured MCLs remained untreated. All the left MCLs were sham operated and used as controls. At 12 weeks, the status of collagen types I and V was evaluated with immunofluorescent staining. The collagen type V/I ratios were obtained using @entity214 -PAGE. Collagen fibril diameters were calculated from the transmission electron micrographs. The results revealed that in the SIS-treated group, the collagen fibers were more regularly aligned as were the cell nuclei. The collagen fibril diameters were 22.2% larger and the ratio of collagen type V/I was 28.4% lower than those for the nontreated group (p < 0.05). These improvements in the morphological characteristics and biochemical constituents of healing MCLs following SIS treatment are the likely reasons for improved mechanical properties.
