The functions of a XXXX phospholipase D alpha gene (CsPLDa) in growth and tolerance to hyperosmotic stress.

@entity2272 @entity286 @entity2271 @entity8558 @entity9688 @entity4202 @entity164 @entity970 @entity968
UNASSIGNED: Plant phospholipase D (PLD), which can hydrolyze membrane phospholipids to produce @entity8558 (PA), a secondary signaling molecule, has been proposed to function in diverse plant stress responses. In this research, a qRT-PCR analysis indicated that the expression of a @entity9688 phospholipase D alpha gene (CsPLDa) was induced by salt and drought stresses in the roots and leaves. To further study the roles of CsPLDa in regulating plant tolerance to salt, @entity968 ( @entity970 ) and @entity2271 ( @entity2272 ) stresses, transgenic @entity286 plants constitutively overexpressing CsPLDa were produced. A qRT-PCR analysis showed that the CsPLDa transcript levels were high in transgenic @entity286 lines, whereas no expression was found in @entity164 ( @entity164 ) @entity286 , indicating that CsPLDa was successfully transferred into the @entity286 genome and overexpressed. Under normal conditions for 30  d, seeds of transgenic lines germinated neatly, and the seedlings were robust and bigger than @entity164 plants. When treated with different concentrations of @entity4202 , @entity970 and @entity2272 , germination rates and seedling sizes of the transgenic lines were significantly greater than @entity164 . In addition, the germination times for transgenic lines were also remarkably shorter. Further studies indicated that transgenic lines had longer primary roots and more biomass accumulation than @entity164 plants. The water loss in transgenic lines was also much lower than in @entity164 . These findings suggest that the CsPLDa overexpression positively regulates plant tolerance to hyperosmotic stresses, and that CsPLDa is involved in the @entity2272 regulation of stomatal closure and the alleviation of @entity2272 inhibition on seed germination and seedling growth.
