Induction of XXXX 70 mRNA by @entity3409 is mediated by @entity2041 suppressive and non-suppressive triggers.

@entity1 @entity2285 @entity43840 @entity1457 @entity26 @entity2041 @entity3409 @entity2197 @entity273 @entity8584 @entity3016 @entity224 @entity71202
The induction mechanism of @entity1457 70 ( @entity2285 ) gene by @entity3409 was investigated. In @entity1 amniotic WISH cells, @entity2285 was induced by @entity3409 in a dose- and time-dependent manner. @entity3409 -induced @entity2285 mRNA levels were enhanced 3- to 4-fold after depletion of intracellular @entity2041 ( @entity2197 ) by either @entity43840 or @entity3016 . Under these conditions, @entity273 might increase in the absence of substrate for @entity2041 peroxidase. We found that exogenous @entity273 alone induced @entity2285 which was further enhanced significantly after @entity2197 -depletion by diethylmaleate. On the other hand, treatment of cells by @entity8584 , an inhibitor of @entity224 dismutase, induced @entity2285 2-fold over the level of control. This induction was further stimulated by @entity3409 even in the presence of @entity2197 . Furthermore, a 4-fold increase of intracellular @entity2197 by the treatment of cells with @entity71202 did not diminish the @entity3409 -induced @entity2285 . Gel mobility shift assays of nuclear extracts, from these differently treated cells, with oligonucleotide containing a promoter region of @entity2285 gene revealed that the levels of @entity2285 mRNA observed in the present study corresponded to the changes of transcription. These results imply that the induction of @entity2285 mRNA by @entity3409 is mediated at least partly via reactive @entity26 species and attenuated by cellular @entity2197 and that some part of @entity3409 -induced @entity2285 can not be eliminated by @entity2197 , suggesting that multiple signals are functioning for this induction.
