XXXX photonic microbioreactors based on segmented waveguides for local absorbance measurement.

@entity25537 @entity16549 @entity941 @entity13768 @entity375
We present the development of microbioreactors (MBRs) based on @entity13768 ( @entity16549 ) segmented waveguides (SWG) for local absorbance measurements. Two different MBRs were studied, either using symmetric or asymmetric SWG (being defined as MBR-S and MBR-A, respectively). Their optical and fluidic performances were numerically analyzed, showing robustness from an optical point of view and distinct fluid flow profile. The optical characterization was done in two steps. Initially, the experimental limit of detection (LOD) and the sensitivity were determined for two different analytes ( @entity941 and methylorange). With both systems, a similar limit of detection for both analytes was obtained, being in the micromolar level. Their sensitivities were 20.2  0.3 ( 10     ) A.U./ M and 5.5  0.2 ( 10     ) A.U./ M for @entity941 and @entity25537 , respectively. Once validated its applicability for local absorbance measurements, a continuous cultivation of @entity375 was done to test the viability of the proposed systems for photonic MBRs. Concretely, the cell growth was locally monitored inside the MBR during 33   h. Spectral analysis showed that the determination of the culture parameters were wavelength dependant, with a growth rate of 0.39  0.02   h      and a doubling time of 1.65  0.09   h at an optimal wavelength of 469.9  0.3   nm. Besides the easy and monolithic integration of the SWG into @entity13768 microfluidic systems, the results presented here are very promising for the application in any disposable photonic lab-on-a-chip systems used for online analysis or photonic MBRs.
