Dual Functions in Response to Heat Stress and Spermatogenesis: Characterization of Expression Profile of Small Heat Shock Proteins 9 and 10 in XXXX Testis.

@entity3930 @entity71548 @entity1237 @entity71547
UNASSIGNED: Small heat shock proteins 9 and 10 ( @entity71547 and HSPB10) are two testis-specific expressed sHsps. The objective of this study was to investigate the mRNA expression profile of @entity71547 and HSPB10 in @entity1237 testis among the different seasons, ages, and environmental temperatures. Allocation of the two sHsps was also performed by immunohistochemistry. The results showed that the transcript levels of @entity71547 and HSPB10 were extremely high in the testis (P < 0.01). The relative expression of HSBP9 and HSPB10 in testis showed a tendency to increase with age and then is maintained at the constant level after sexual maturity. @entity71547 and HSPB10 have significantly higher expression in the breeding season      (P < 0.05) and hot season (P < 0.01). Both @entity71547 and HSPB10 were found to be upregulated by high-temperature stress in testis (P < 0.05), and the expressions of @entity71548 and Hsp90 were also increased simultaneously (P < 0.01). Immunohistochemistry analysis localized @entity71547 expressed in spermatogonia, spermatocytes, and round spermatids and HSPB10 expressed in the elongate spermatids. In epididymis, strongly staining signal of HSPB10 was detected in pseudostratified columnar epithelium. In conclusion, the two testis-specific sHsps are closely related to @entity3930 . The results could provide valuable data for the further studies on @entity71547 and HSPB10.
