Effect of antifreeze protein supplementation in vitrification medium on XXXX oocyte developmental competence.

@entity43487 @entity19 @entity43485 @entity43486 @entity21032 @entity43488 @entity27211 @entity21030 @entity21031 @entity855
OBJECTIVE: To investigate the effect of antifreeze protein (AFP) supplementation during @entity19 oocyte vitrification on the survival, fertilization and embryonic development. DESIGN: Animal study. SETTING: University laboratory. ANIMAL(S): BDF-1 @entity19 . INTERVENTION(S): In  vivo-matured metaphase II oocytes were vitrified with the use of CryoTop by two-step exposure to equilibrium and vitrification solution supplemented or not with 500 ng/mL AFP III. MAIN OUTCOME MEASURE(S): Postwarming survival, fertilization, embryonic development up to blastocyst in  vitro, morphology of spindle and chromosome, membrane integrity, @entity855 ( @entity855 ) contents, and several gene expressions. RESULT(S): In the AFP-treated group, blastocyst formation rate was significantly higher and blastomere count with positive caspase was significantly lower compared with the nontreated group. Rate of intact spindle/chromosome, stable membrane, and @entity855 contents were significantly higher in AFP group. AFP group showed higher @entity43485 and lower @entity43488 gene expression. Both vitrification groups showed increased @entity27211 , @entity43487 , and @entity21030 / @entity21031 expression and decreased @entity43486 and @entity21032 expression compared with fresh control samples. CONCLUSION(S): Supplementation of AFP in vitrification medium has a protective effect on @entity19 oocytes for chilling injury; it can preserve spindle/membrane integrity and intracellular @entity855 contents. More stable spindle integrity in the AFP group may be associated with higher @entity43485 and lower @entity43488 gene expression.
