Genotypic analysis of XXXX .

@entity1 @entity5 @entity1222 @entity406 @entity982
Reported studies show that the systemic form of @entity1222 ( @entity1222 ) is a clonal expansion of Langerhans cells (LC) associated with aberrant expression of several oncogenes or @entity5 -suppressor genes. @entity1222 of the lung is a heterogenous group of lesions thought to be a reactive rather than @entity5 . The histogenesis of the @entity1222 of the lung is uncertain, and to date there are no studies investigating its underlying molecular abnormalities. We performed comparative genotypic analysis by using allelic loss (LOH) of polymorphic microsatellite markers associated with @entity5 suppressor genes. Fourteen cases of @entity406 -fixed, @entity982 -embedded @entity1222 of the lung were studied. Microdissection of a total of 26 nodules from 14 @entity1 and paired reference lung tissue was performed under stereomicroscopic visualization. To evaluate allelic loss, we used a panel of 11 polymorphic microsatellite markers that were situated at or near @entity5 suppressor genes on chromosomes 1p, 1q, 3p, 5p, 9p, 17p, and 22q. The PCR products were analyzed by using capillary electrophoresis to identify germline heterozygous alleles and LOH. Allelic loss at 1 or more @entity5 suppressor gene loci was identified in 19 of 24 nodules. The total fractional allelic loss (FAL) ranged from 6% (1q) to 41% (22q), with a mean of 22%. The FAL in individual cases ranged from 0 (7 nodules) to 57% (1 nodule). Fifteen discordant allelic losses at 1 to 3 chromosomal loci were identified in 8 @entity1 with multiple synchronous nodules. Our results show that LOH of @entity5 suppressor genes is present in the @entity1222 of the lung, and they indicate that the putative @entity5 suppressor genes situated on chromosomes 9p and 22q may play a role in the development of a subset of the @entity1222 of the lung.
