Expression of XXXX , in primary cultures of human prostatic stromal and epithelial cells.

@entity1552 @entity5379 @entity1476 @entity5 @entity1067 @entity442 @entity540 @entity4179 @entity2533 @entity2843 @entity1495 @entity16617
The production of matrix metalloproteinases (MMPs) by prostatic epithelial and/or neighboring stromal cells is considered to be a property that gives cells the capability to penetrate extracellular matrix barriers in normal or @entity5 . In order to examine the role of MMPs in the prostate, we evaluated the expression of @entity5379 and the @entity16617 in primary cultures of prostatic stromal and epithelial cells. These cells were isolated from normal tissues of the different zones of the prostate, from @entity1067 ( @entity1067 ) and from @entity5 . Stromal cells, regardless of tissue of origin, secreted the 72-kDa proenzyme form of @entity1476 , whereas conditioned media (CM) from epithelial cells demonstrated little/no pro- @entity1476 as examined by zymography. Either type of cell did not secrete @entity1495 . RT-PCR evaluation showed stromal cells expressed transcripts for @entity1476 , but not for @entity1495 . Transcripts for @entity1495 were detected in epithelial cells, although no @entity1495 activity was detected in their CM. Treatment of stromal cells with 1 or 10 ng/ml of @entity2533 ( @entity2533 ) moderately increased secretion of pro- @entity1476 protein with little change in @entity1476 RNA. However, treatment of epithelial cells with @entity2533 induced expression and secretion of both @entity5379 . The effect of @entity2533 on expression of MMPs by epithelial cells was not duplicated or affected by treatment with @entity442 , @entity4179 ( @entity4179 ), platelet-derived growth factor (PDGF), or @entity540 ( @entity540 ). Stromal cells expressed transcripts of both @entity16617 . Epithelial cells expressed @entity2843 , but little @entity1552 . @entity2533 did not regulate the expression of @entity2843 or -2 in either stromal or epithelial cells. Our results suggest that the elevated levels of @entity5379 observed in prostate development and @entity5 may be due to the elevated @entity2533 associated with these tissues.
