The properties of bovine serum albumin and chymotrypsinogen A in solvent mixtures containing XXXX .

@entity3971 @entity717 @entity1543
1. The @entity717 and reduced viscosity of bovine serum albumin and chymotrypsinogen A in solvents containing @entity3971 , @entity1543 and water were studied. 2. The changes brought about in the properties of the proteins by varying the composition of the solvent or by heat treatment in these solvents were established to be reversible. 3. A method for returning the proteins to aqueous media, based on these observations, was worked out. 4. The recovered proteins were shown to be very similar to, if not identical with, the native proteins on the basis of measurements of @entity717 , viscosity, sedimentation, ultraviolet spectroscopy, immunochemical behaviour (serum albumin) and proteolytic activity (chymotrypsinogen A, after activation with trypsin). 5. The importance of the findings for partitioning of polyelectrolytes in the @entity3971 -aqueous buffer systems is discussed.
