Aggravation of @entity3034 -induced @entity2359 by XXXX in PC12 cells.

@entity1 @entity3034 @entity137 @entity7312 @entity1911 @entity26 @entity898 @entity191 @entity855 @entity98 @entity35 @entity17057 @entity2359
@entity17057 ( @entity7312 ) is formed in @entity191 @entity1 receiving @entity3034 therapy and is detected in the plasma and urine of these @entity1 . In this study, we have investigated the effects of @entity7312 on @entity3034 -induced @entity2359 in cultured @entity35 @entity1911 , PC12 cells. Exposure of PC12 cells up to 10 microM @entity7312 or 20 microM @entity3034 after 24 or 48 hr, neither affected the cell viability determined by @entity98 assay, nor induced apoptosis by flow cytometry and TUNEL staining. However, at concentrations higher than 15 microM, @entity7312 showed @entity137 through an apoptotic process. In addition, @entity7312 at 5-15 microM for both incubation time points significantly enhanced @entity3034 -induced @entity2359 ( @entity3034 concentration, 50 microM). Exposure of PC12 cells to @entity7312 , @entity3034 and @entity7312 plus @entity3034 for 48 hr resulted in a marked increase in the cell loss and percentage of apoptotic cells compared with exposure for 24hr. The enhancing effects of @entity7312 on @entity3034 -induced @entity2359 were concentration- and treated-time-dependent. @entity7312 , @entity3034 and @entity7312 plus @entity3034 produced a significant increase in intracellular reactive @entity26 species generation and decrease in @entity855 levels, supporting the involvement of oxidative stress in @entity7312 - and @entity3034 -induced apoptosis. The antioxidant @entity898 strongly inhibited changes in apoptosis, decreases in cell viability and ROS generation induced by @entity7312 associated with @entity3034 . These results suggest that @entity7312 aggravates @entity3034 -induced oxidative neurotoxic and apoptotic effects in PC12 cells. Therefore, @entity191 @entity1 treated with @entity3034 for long-term need to be monitored for the relationship between plasma concentration of @entity7312 and the symptoms of @entity2359 .
