Identification of early molecular markers for XXXX .

@entity1 @entity3821 @entity0 @entity73147 @entity19 @entity5 @entity2153 @entity14633 @entity44915 @entity189 @entity73149 @entity1189 @entity513 @entity73148
BACKGROUND: The @entity3821 ( @entity3821 ) of the mammary gland represents an early, pre-invasive stage in the development of @entity0 . Since @entity3821 is a curable disease, it would be highly desirable to identify molecular markers that allow early detection. @entity19 transgenic for the WAP-SV40 early genome region were used as a model for @entity3821 development. Gene expression profiling was carried out on @entity3821 -bearing @entity19 and control animals. Additionally, a set of @entity1 @entity3821 and invasive @entity5 were analyzed in a similar fashion. Enhanced expression of these marker genes in @entity1 and @entity19 samples was validated by quantitative RT-PCR. Besides, marker gene expression was also validated by immunohistochemistry of @entity1 samples. Furthermore in silico analyses using an online microarray database were performed. RESULTS: In @entity3821 - @entity19 seven genes were identified that were significantly up-regulated in @entity3821 : @entity73147 , @entity73148 , @entity73149 , @entity2153 , @entity14633 , @entity44915 and @entity1189 . A similar up-regulation of homologues of the @entity19 genes was observed in @entity1 @entity3821 samples. Enhanced expression of these genes in @entity3821 and @entity513 ( @entity513 ) was validated by quantitative RT-PCR and immunohistochemistry. CONCLUSIONS: By comparing @entity19 markers for the @entity3821 ( @entity3821 ) of the mammary gland with genes up-regulated in @entity1 @entity3821 -samples we were able to identify a set of genes which might allow early detection of @entity3821 and invasive @entity189 in the future. The similarities between gene expression in @entity3821 and invasive @entity189 in our data suggest that the early detection and treatment of @entity3821 is of utmost relevance for the survival of @entity1 who are at high risk of developing @entity0 .
