TRYPTASE AND XXXX MAY SUPPORT ORAL FOOD CHALLENGE IN THE DIAGNOSIS OF ALLERGY.

@entity1 @entity1697 @entity5419 @entity56 @entity112 @entity9416
Allergen-specific @entity9416 ( @entity9416 ) reactions lead to acute degranulation of mast cells and basophils and release of stored mediators, particularly tryptase and @entity112 , which can be measured in vitro after reactions. The aim of this study was to investigate the utility of serum tryptase and plasma @entity112 during @entity1697 ( @entity1697 ) in 103 @entity1 with suspected @entity5419 , in order to support the diagnosis of a @entity9416 -mediated reaction. Blood samples for serum tryptase and plasma @entity112 were collected before the @entity1697 and after the onset of @entity56 or after 60 minutes from test completion. Serum tryptase and plasma @entity112 were measured by a fluoroenzyme immunoassay (ImmunoCAP; ThermoFisher, Uppsala, Sweden) according to the manufacturer  s instructions. A correlation between serum tryptase and plasma @entity112 distributions was observed after @entity1697 (p=0.0035). A correlation was also observed for both serum tryptase and plasma @entity112 before and after @entity1697 (p less than0.0001). Subjects with positive response to @entity1697 had significantly higher values (p = 0.0375) of serum tryptase compared to subjects with negative response. The plasma @entity112 distribution showed a significant difference between measurements before and after @entity1697 , both in the complete population (p less than 0.0001), and considering the response (negative @entity1697 : p less than 0.0001; positive @entity1697 : p=0.0181). The diagnostic work-up of @entity9416 - mediated @entity5419 may include determination of serum tryptase and plasma @entity112 , in order to support the results of @entity1697 . These markers are strongly related to the same @entity9416 -mediated mechanism and, as they can be both easily measured, can confirm the allergic nature of a reaction in the real-life setting of @entity5419 .
