Up-regulation of microRNA-145 promotes differentiation by repressing @entity4729 in @entity1 XXXX cells.

@entity1 @entity1204 @entity4729 @entity5 @entity19 @entity3373
BACKGROUND: MicroRNA-145 ( @entity3373 ) has been reported to be a @entity5 -suppressing agent in several studies. It can repress pluripotency and control @entity1 embryonic stem cell differentiation by regulating the core pluripotency factor @entity4729 . However, it is not known whether @entity3373 can play a role in inducing @entity5 cell differentiation and repressing growth of @entity5 . METHODS: Ishikawa cells, the established @entity1 @entity1204 cells, were treated with @entity3373 mimics, inhibitor, or small interfering RNA @entity4729 . @entity3373 levels were assayed using TaqMan microRNA assays, and the messenger RNA levels of @entity4729 and the differentiation marker glycodelin were measured using real-time polymerase chain reaction. The protein levels of @entity4729 and glycodelin were characterized via flow cytometry, western blotting, and immunohistochemistry. In vivo activity was measured in a xenograft @entity19 model. RESULTS: Up-regulating @entity3373 reduced the expression of @entity4729 and induced the differentiation of Ishikawa cells to closely resemble normal endometrial epithelium both in vitro and in vivo. @entity3373 successfully inhibited @entity5 growth. We also found that in @entity1 with @entity1204 , @entity3373 and @entity4729 were expressed in tissues, and there was a relationship between @entity3373 , @entity4729 , and the degree of @entity5 cell differentiation. CONCLUSIONS: Our results strongly suggested that @entity3373 is a @entity5 cell differentiation-inducing agent in @entity1204 , and that @entity3373 or @entity4729 may be useful markers for grading @entity1204 .
