Apoptotic cell death and genetic control in graft XXXX in heart transplant.

@entity1 @entity453 @entity588 @entity876
AIM: Apoptosis is a type of programmed cell death whereby, immunologic, genetic and biochemical mechanisms are involved in its control. On the other hand, graft @entity453 is the most important restrictive factor for the long-term survival of heart transplantation. The purpose of this study is to analyse both apoptotic cell lesions in transplanted @entity1 that present @entity453 . METHODS: From August 1984 until December 1996, 148 heart transplants were carried out in the Cl  nica Universitaria de Navarra. In 102 @entity1 , annual coronary angiography was performed, reaching a diagnosis of @entity453 in 30 @entity1 . Study of apoptotic cell death was done in the tissue of endomyocardial biopsies on all @entity1 by means of the TUNEL technique. Procedures of immunohistochemistry with antibodies antic-myc, @entity876 and @entity588 were carried out and results were compared with a control group of 30 @entity1 with homogeneous characteristics. RESULTS: All @entity1 with @entity453 showed apoptotic cardiomyocytes, 13 @entity1 to a mild degree, 14 to a moderate degree and 3 to a severe degree, while in the control group apoptosis was found only to a mild degree in 8 @entity1 , obtaining a very significant statistical difference (p<0.0001). The expression of analysed oncoproteins was null in the 2 groups. CONCLUSION: Myocardial apoptosis is a constant finding in transplanted @entity1 with @entity453 . We have not seen any correlation between the apoptotic process and genetic mechanisms.
