Characteristics of three XXXX @entity356 cell lines, NU-GC-2, NU-GC-3 and NU-GC-4.

@entity1 @entity632 @entity5 @entity1138 @entity2580 @entity3 @entity420 @entity356
Three @entity1 @entity356 cell lines, NU-GC-2, NU-GC-3 and NU-GC-4 were established in vitro from the @entity5 tissues obtained from 3 @entity1 during surgery. The pathological findings of the @entity356 of these cases revealed poorly differentiated @entity420 (and partial @entity2580 in the case of NU-GC-4). NU-GC-2 and NU-GC-4 were originally obtained from metastatic paragastric lymph nodes and NU-GC-3 was obtained from a @entity3 in the brachial muscle. The cells of NU-GC-2 and NU-GC-3 are polygonal in shape and grow as a monolayer sheet. NU-GC-4 cells, however, are mainly spherical in shape with a few free floating cells. Electron microscopy revealed epithelial characteristics in all 3 cell lines. The average doubling time of NU-GC-2 was 36.1 hours, that of NU-GC-3 was 38.2 hours and that of NU-GC-4 was 29.9 hours. The modal chromosome number of NU-GC-2 was 62, that of NU-GC-3 was 58 and those of NU-GC-4 grown in in vitro and in vivo were 52-54 and 53, respectively. In vitro and in vivo lines of NU-GC-4 were established from the same @entity5 . These two cell lines are quite similar in morphology, but slightly different in karyotype. The in vitro sensitivity to anticancer agents was highest in NU-GC-4 and lowest in NU-GC-2. Of the anticancer agents, @entity1138 and @entity632 were most effective on the cells of all 3 cell lines.
