Molecular cloning and characterization of the XXXX Acdp gene family.

@entity1 @entity1760 @entity1522 @entity19 @entity70873 @entity535 @entity377 @entity1953 @entity57449 @entity70875 @entity70874
BACKGROUND: We have recently cloned and characterized a novel gene family named ancient conserved domain protein (ACDP) in @entity1 . To facilitate the functional study of this novel gene family, we have cloned and characterized Acdp, the @entity19 homologue of the @entity1 ACDP gene family. RESULTS: The four Acdp genes ( @entity70873 , @entity57449 , @entity70874 and @entity70875 ) contain 3,631 bp, 3,244 bp, 2,684 bp and 2,743 bp of cDNA sequences, and encode deduced proteins of 951, 874, 713 and 771 @entity1522 , respectively. The @entity19 Acdp genes showed very strong homologies (>90%) in both @entity377 and @entity535 sequences to their @entity1 counterparts. In addition, both @entity377 and @entity535 sequences within the Ancient Conserved Domain (ACD) are highly conserved in many different taxonomic species. Particularly, Acdp proteins showed very strong AA homologies to the bacteria CorC protein (35% AA identity with 55% homology), which is involved in @entity1760 and @entity1953 efflux. The Acdp genes are widely expressed in all tissues tested except for @entity70873 , which is only highly expressed in the brain with low levels of expression in kidney and testis. Immunostaining of @entity70873 in hippocampus neurons revealed a predominant localization on the plasma membrane. CONCLUSION: The Acdp genes are evolutionarily conserved in diverse species and ubiquitously expressed throughout development and adult tissues suggesting that Acdp may be an essential gene. Acdp showed strong homology to bacteria CorC protein and predominantly localized on the plasma membrane. These results suggest that Acdp is probably a family of proteins involved in ion transport in @entity1 cells
