Expression of trefoil peptides ( @entity3559 , @entity3560 , and @entity3561 ) in @entity356 , XXXX , and non-neoplastic gastric tissues.

@entity1 @entity1944 @entity5 @entity33 @entity3561 @entity356 @entity3559 @entity3560
Trefoil factor family (TFF) domain peptides consist of three members that play a @entity33 and repair, and in tumourigenesis. The role of the three TFF members in the @entity356 cascade remains poorly defined. This study examined seven gastric cell lines, 50 @entity356 and their adjacent non- @entity5 tissues, and tissues from 40 non- @entity5 @entity1 , in order to elucidate the chronology of TFF expression in various stages of @entity356 . TFF expression was determined by RT-PCR, immunohistochemistry, and western blot. Aberrant expression of @entity3559 , @entity3560 , and @entity3561 was frequently detected in gastric cell lines. Specifically, @entity3559 was detected in all non- @entity5 @entity1 , but was detected in only 50% of @entity356 and 66% of adjacent normal tissues. @entity3560 expression was demonstrated in 87.5% of non- @entity5 @entity1 , 34% of @entity356 , and 58% of adjacent non- @entity5 tissues. There was a significant correlation between @entity3559 and @entity3560 expression in @entity356 and adjacent non- @entity5 tissues (p<0.001). By contrast, @entity3561 was detected in 25% of non- @entity5 @entity1 and showed a predilection for areas with @entity1944 (p=0.005). Sixty-two per cent of @entity356 and 24% of neighbouring non- @entity5 tissues showed @entity3561 expression. Immunoreactivity against @entity3561 was demonstrated in goblet cells of @entity1944 and within the cytoplasm and nuclei of @entity5 cells. Progressive loss of @entity3559 and @entity3560 , together with the induction of @entity3561 , is likely to be involved in the early stage of the multi-step @entity356 pathway.
