Characterization of proteins in XXXX roots under flooding and drought stresses.

@entity7726 @entity1460
UNASSIGNED: Flooding and drought affect @entity1460 growth because @entity1460 is a stress-sensitive crop. In 2-day-old plants exposed to 2-day flooding or drought, the fresh weight of roots was markedly suppressed, although the root morphology clearly differed between two conditions. To understand the response mechanisms of @entity1460 to flooding and drought stresses, a gel-free proteomic technique was used. A total of 97 and 48 proteins were significantly changed in response to flooding and drought stresses, respectively. Proteins involved in protein synthesis were decreased by flooding stress and increased by drought. Glycolysis-related proteins were increased in roots by both flooding and drought stresses. Fermentation, stress, and cell wall-related proteins were increased in response to flooding stress, whereas cell organization and redox-related proteins were increased under drought stress. Among the identified proteins, three @entity7726 synthetases were commonly decreased and increased in response to flooding and drought stresses, respectively. The mRNA expression levels of @entity7726 synthetase genes displayed a similar tendency to the changes in protein abundance. These results suggest that @entity7726 synthetase is involved in the regulation of stress response because it was changed in response to flooding and drought stresses. BIOLOGICAL SIGNIFICANCE: This study reported on the response mechanisms of @entity1460 to flooding and drought stresses using the gel-free proteomic technique. Proteins involved in protein synthesis were decreased by flooding stress and increased by drought. Glycolysis-related proteins were increased in roots by both flooding and drought stresses. Fermentation, stress, and cell wall-related proteins were increased in response to flooding stress, whereas cell organization and redox-related proteins were increased under drought stress. Among the identified proteins, three @entity7726 synthetases were commonly decreased and increased in response to flooding and drought stresses, respectively. The mRNA expression levels of @entity7726 synthetase genes displayed a similar tendency to the changes in protein abundance. These results suggest that @entity7726 synthetase is involved in the regulation of stress response because it was changed in response to flooding and drought stresses.
