Molecular study of @entity3377 in XXXX by the use of TaqMan   real-time PCR.

@entity6469 @entity20770 @entity3377 @entity6467
@entity3377 is a zoonosis of economic importance that reduces productivity in livestock enterprises as it induces abortion in infected animals. A study was designed aimed at detecting Brucella in blood and lymph node specimens from @entity20770 by the use of real-time PCR in Iran. Sample collection and DNA extraction were done on blood (n = 135) and lymph node (n = 135) samples collected from 135 @entity20770 (abattoir survey) from both sexes at various ages in different seasons. The real-time PCR for species differentiation was based on unique genetic loci of @entity6467 and @entity6469 . The regions were chosen for the construction of primers and TaqMan   probes for species differentiation: BMEII0466 gene for @entity6467 and Bru-Ab2_0168 gene for @entity6469 . Brucella spp. were identified in 18 (13.33%) blood samples and 4 (2.97%) lymph node samples. This method showed to be effective in detecting @entity6469 and @entity6467 in blood and lymph samples respectively. @entity6469 was detected in 3 (2.22%) blood samples but was however, not detected in the lymph node samples. @entity6467 was only observed in 4 (2.97%) lymph node samples. Significant differences were observed on the blood prevalence of unknown Brucella spp. in different age groups and seasons (P < 0.05). However, there were no significant differences observed on the prevalence of @entity6469 , @entity6467 , unknown Brucella spp. in different age groups, sex and seasons (P > 0.05). Therefore, Brucella was detected in apparent healthy @entity20770 slaughtered at an abattoir in Iran and this recommends the significance of the detection of Brucella in @entity20770 , since the infected @entity20770 appear to be healthy.
