@entity19812 Inhibits @entity63 /Reperfusion-Induced @entity296 in XXXX via Downregulation of microRNA-208b-3p.

@entity72327 @entity296 @entity35 @entity63 @entity2667 @entity1976 @entity2310 @entity12990 @entity2822 @entity519 @entity20784 @entity19812 @entity3096
BACKGROUND: @entity19812 ( @entity20784 ), a kind of @entity3096 which is extracted from a variety of diets, has been reported to convey protective effects of various diseases. Recent researches have suggested that @entity20784 can carry out cardioprotective effects during @entity63 /reperfusion (I/R). However, there have no reports on whether @entity20784 can exert protective effects against @entity296 /R injury through the actions of specific microRNAs (miRs). The purpose of this study was to determine which miRs and target genes @entity20784 exerted such function through. METHODS: Expression of various miRs in perfused @entity35 hearts was detected using a gene chip. Target genes were predicted with TargetScan, MiRDB and MiRanda. @entity519 /reoxygenation was used to simulate I/R. Cells were transfected by @entity72327 -3p mimic, inhibitor and small interfering RNA of @entity12990 (avian erythroblastosis virus E26 (v ets) oncogene homolog 1). @entity72327 -3p and @entity12990 mRNA were quantified by real-time quantitative polymerase chain reaction. The percentage of apoptotic cells was detected by annexin V- @entity2822 / @entity2667 dyeing and flow cytometry. The protein expression levels of cleaved caspase-3, @entity1976 , @entity2310 , and @entity12990 were examined by western blot analysis. A luciferase reporter assay was used to verify the combination between @entity72327 -3p and the 3'-untranslated region of @entity12990 . RESULTS: @entity20784 pretreatment reduced @entity72327 -3p expression in myocardial tissue, as compared to the I/R group. And @entity20784 decreased @entity72327 -3p expression and apoptosis caused by I/R. However, overexpression of @entity72327 -3p further aggravated the changes caused by I/R and blocked all the effects of @entity20784 . Knockdown of @entity72327 -3p expression also attenuated apoptosis, while knockdown of @entity12990 promoted apoptosis. Further, the luciferase reporter assay showed that @entity72327 -3p could inhibit @entity12990 expression. CONCLUSION: @entity20784 pretreatment conveys anti-apoptotic effects after @entity296 /R injury by decreasing @entity72327 -3p and increasing @entity12990 expression levels.
