Contraction-related factors affect the concentration of a XXXX -like peptide in @entity35 muscle tissue.

@entity859 @entity5834 @entity880 @entity17230 @entity1812 @entity35 @entity19794
In order to study the effects of the manipulation of various factors related to muscular activity on the concentration of kinins in muscular tissue, a microdialysis probe was implanted in the adductor muscle of the hindlimb in anaesthetized @entity35 . After collection of baseline samples, the perfusion fluid was changed to a Ringer solution containing @entity19794 (10 or 20 mM), @entity859 (50 or 100 microM) or a lower pH (7.0 or 6.6). Whereas perfusion with @entity880 did not have any significant effect on the concentration of kinins in the dialysate, the perfusion with a lower pH or with @entity859 dose-dependently increased the kinin content in the samples. In a second microdialysis experiment, by using specific radioimmunoassays (RIA) for @entity1812 and @entity17230 , we observed that about 70 % of the total kinins dialysed from @entity35 muscle are a @entity17230 -like peptide. Also, the simultaneous perfusion with 100 microM @entity5834 totally abolished the increase in kinin levels induced by the perfusion at pH 6.6. In a third experiment, soleus muscles from @entity35 were stimulated in vitro during 30 min in the presence or absence of 77 microM @entity5834 . Electrically stimulated contraction, but not the addition of 10 mU ml(-1) insulin, induced an increase in the concentration of the @entity17230 -like peptide in the buffer. This effect was totally prevented by the addition of the @entity859 antagonist @entity5834 . These results show that a @entity17230 -like peptide is released from @entity35 muscle, and that its production is enhanced by muscle activity. Furthermore, the increase in kinin peptides during muscle contraction may be mediated by an increase in @entity859 levels.
