Gene transfer mediated by different viral vectors following direct cannulation of XXXX submandibular salivary glands.

@entity32 @entity1703 @entity19 @entity281 @entity1800 @entity19786
The salivary gland has been suggested as an accessible organ for gene transfer to express recombinant proteins locally in the saliva, as well as for secretion to the blood circulation. The aim of this study was to evaluate the efficiency of gene transfer to salivary glands using different viral vectors: adenovirus, @entity19786 , @entity1703 (HSV), and two retroviral vectors ( @entity19 leukemia virus (MuLV) and lentivirus). We show, by in situ staining and beta-galactosidase reporter activity assay, that the adenoviral and @entity19786 vectors were able to deliver the reporter gene efficiently to acinar and duct cells. The HSV vector was less efficient and infected only the acinar cells. The lentiviral vector infected acinar and duct cells, but at a relatively low efficiency. The MuLV vector did not infect the salivary gland unless cell proliferation was induced. Host immune responses to @entity1800 , @entity32 , apoptosis and lymphocyte infiltration, in the transduced glands, were assessed. The DNA viral vectors induced local lymphocyte infiltration and apoptosis. In contrast, the retroviral vectors did not induce an immune response. Our results describe the outcome of @entity281 with each of the five different viral vectors and indicate their advantages and limitations for transferring genes to the salivary glands.
