An ultrastructural and biochemical study of the effects of three inhibitors of @entity165 biosynthesis upon XXXX adrenal gland and testis. Histochemical evidence for a lysosome response.

@entity71250 @entity5068 @entity33255 @entity14952 @entity19 @entity71248 @entity19980 @entity3150 @entity71249 @entity165
@entity71248 and @entity71249 inhibit @entity165 biosynthesis and result in the accumulation of @entity19980 . @entity33255 , another inhibitor, produces an accumulation of @entity14952 . Adult male C3H @entity19 receive one of these drugs intraperitoneally. Livers, adrenal glands, and testes from each drug group are excised, and portions of each are analyzed by a modified Liebermann-Burchard reaction for quantitation of @entity3150 . Adrenals and testes are examined also by electron microscopy. Fine-structural localization of acid phosphatase has been studied in @entity71248 -treated adrenal glands. Biochemical analysis reveals that 14-64% of the @entity3150 occurs as @entity19980 or @entity14952 . Fine-structural alterations in the adrenal glands and testes from each drug group are essentially identical. The predominant cytological feature is the occurrence of increased numbers of pleomorphic, unit-membrane-limited, electron-opaque, cytoplasmic inclusions. Hence, the cellular modifications following @entity71248 administration are not unique, as has been suggested. They represent a generalized phenomenon, probably related to inhibition of @entity165 biosynthesis, which is an effect common to each drug. @entity71250 reaction product (indicating acid phosphatase activity) is demonstrable within these membrane-limited cytoplasmic bodies, identifying them as morphological lysosomes. The utilization of a lysosomal mechanism in @entity5068 -synthesizing cells, which are accumulating @entity165 intermediates, is discussed.
