Transformation potential of bone marrow stromal cells into undifferentiated high-grade XXXX .

@entity242 @entity5 @entity19 @entity185 @entity3178 @entity6580 @entity27063 @entity5954 @entity3844
PURPOSE: Bone marrow adherent cells contain conventional bone marrow stromal cells and mesenchymal stem cells and these cells constitute the hematopoietic microenvironment. Mesenchymal stem cells have the capacity to give rise to multiple mesenchymal lineage cells and even ectodermal lineage cells. In the present study, we investigated what types of @entity5 cells are inducible from BM adherent cells by chemical carcinogens. METHODS: Bone marrow cells from neonatal C3H/HeN @entity19 were collected within 24 h after birth and then cultured. Four days later, bone marrow adherent cells were obtained and the cells were treated with @entity5954 . RESULTS: By this treatment, some transformed clones consisting of large spindle cells were obtained. The transformed cells were highly positive for @entity3844 and were positive for @entity185 , @entity27063 and @entity6580 , whereas the cells were negative for hematolymphoid markers. The cell clones had the ability to support hematopoiesis in vitro. These results indicate that the transformed cell lines have the characteristics of BM stromal cells/mesenchymal stem cells. Moreover, during culture of the transformed cells, spontaneous bone nodule formation was observed. When the transformed cells were inoculated into immunodeficient @entity19 subcutaneously, the @entity5 grew in the subcutaneous tissue of the @entity19 . @entity3178 and ultrastructurally, the @entity5 showed the typical morphology of undifferentiated high-grade @entity242 (UHGPS). Bone-related genes have been found to be expressed in both transformed cells and UHGPSs. CONCLUSION: The present study suggests that UHGPSs are derived from BM stromal cells, probably mesenchymal stem cells.
