Immunohistochemical characterization of two novel monoclonal antibodies that recognize XXXX perivascular cells of the central nervous system and macrophage subsets.

@entity1 @entity1357 @entity406 @entity1735 @entity15328 @entity982 @entity15327
Two monoclonal antibodies to cells of monocyte/macrophage lineage were established using a @entity1 glial cell-rich fraction as the immunogen. The antibodies, named @entity15327 and @entity15328 , were originally found to react with perivascular cells of the central nervous system. They are immunohistochemically applicable on routinely @entity406 -fixed, @entity982 -embedded tissue sections. @entity15327 binds to a lysosomal protein, and @entity15328 to a @entity1735 epitope of the cell membrane and lysosomes. Among the visceral organs, @entity15327 labeled blood monocytes, almost all kinds of tissue and infiltrating macrophages in both normal and diseased states, and renal tubules. @entity15327 staining of tissue macrophages tends to be intensified under inflammatory conditions. @entity15327 staining also suggested that perivascular cells and macrophages had different ontogeny. @entity15328 immunostained monocytes, Kupffer's cells, red pulp macrophages, infiltrating macrophages and reactive microglia, but not alveolar or tingible body macrophages. Besides those macrophages, @entity15328 stained mantle zone lymphocytes, some hematopoietic cells, pneumocytes and renal collecting ducts. The staining pattern of ligands on @entity1357 and HL-60 neoplastic @entity1 macrophage cell lines was dissimilar to that of other macrophage markers, suggesting that they recognize unknown macrophage-related antigens.
