The Light Wavelength Affects the Ontogeny of Clock Gene Expression and Activity Rhythms in XXXX Larvae.

@entity73181 @entity336 @entity41115 @entity41117 @entity41118 @entity3392
UNASSIGNED: Light plays a key role in synchronizing rhythms and setting the phase of early development. However, to date, little is known about the impact of light wavelengths during the ontogeny of the molecular clock and the behavioural rhythmicity. The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene ( @entity41117 , @entity41118 , clock1, @entity41115 and @entity73181 ) expression rhythms. For this purpose, 4 groups of @entity3392 embryo/larvae were raised from 0 to 7 days post-fertilization (dpf) under the following lighting conditions: three groups maintained under light:dark (LD) cycles with white (full visible spectrum, LDW), blue (LDB), or red light (LDR), and one group raised under constant darkness ( @entity336 ). The results showed that lighting conditions influenced activity rhythms. Larvae were arrhythmic under @entity336 , while under LD cycles they developed wavelength-dependent daily activity rhythms which appeared earlier under LDB (4 dpf) than under LDW or LDR (5 dpf). The results also revealed that development and lighting conditions influenced clock gene expression. While clock1 rhythmic expression appeared in all lighting conditions at 7 dpf, @entity41117 , @entity41118 and @entity73181 showed daily variations already at 3 dpf. Curiously, @entity41115 showed consistent rhythmic expression from embryonic stage (0 dpf). Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR. These results emphasize the importance of lighting conditions and wavelengths during early development for the ontogeny of daily rhythms of gene expression and how these rhythms are reflected on the behavioural rhythmicity of @entity3392 larvae.
