Migration and differentiation of bone marrow lymphocytes: development of surface immunoglobulin, XXXX , complement receptor, and functional responsiveness as studied with anti-allotype serum.

@entity37874 @entity742 @entity19 @entity2822 @entity6433
Immunofluorescent studies using @entity2822 -conjugated mouse anti-allotype antibody were carried out to study the migration pattern and the development of surface Ig (SIg), @entity6433 for IgG ( @entity37874 ), and complement receptor (CR) or @entity19 bone marrow lymphocytes following intravenous injection into congenic @entity19 . After transfer of bone marrow cells from CSW @entity19 into untreated congenic CWB @entity19 , the absolute number of donor-type SIg-bearing (SIg+) cells and the proportion of either @entity37874 - or CR-bearing ( @entity37874 + or CR+) cells in donor-type SIg+ cells were evaluated in the recipient spleen and the results were compared with those obtained after the transfer of CSW spleen cells. After injection of donor bone marrow cells, detectable donor-type SIg+ cells, although few initially, increased from day 1 to Day 2 and reached a plateau thereafter. The proportion of @entity37874 + cells in donor-type SIg+ cells, although very low in the donor marrow inoculum, increased progressively after 1 day to reach a maximum at Day 5 (90%). On the other hand, following the transfer of spleen cells, the proportion of @entity37874 + cells remained at high levels (90%) for 5 days after transfer. Likewise, the proportion of CR+ cells in donor-type SIg+ cells was very low (less than 1%) in the original donor bone marrow cells but high (60%) in the donor spleen cells. However, in transferring bone marrow cells this proportion also increased in the recipient spleen to reach a maximum (49%) at Day 5 although it was lower compared to the percentage of @entity37874 + cells in donor SIg+ cells. Furthermore, the ability of functional responsiveness to antigen was also examined in the same system by detecting plaque-forming cells (PFC) from donor origin. In transferring donor bone marrow cells into recipient, the participation of donor cells in the PFC response was very low when the recipients were primed with @entity742 red blood cells at Day 3 after transfer. However, when the recipients were primed at Days 7 to 21 after transfer, increasing numbers of the donor marrow-derived cells were involved in the PFC response. Thus, the present study demonstrates that the bone marrow-derived lymphocytes, albeit lacking both distinctive surface receptors (IgM, @entity37874 , CR) and the functional responsiveness to antigen, continue their development along the B-cell lineage after migrating into the spleen, as evidenced by the surface receptor expression and participation in the antibody response.
