[The effects of Kangai injection on enzyme activities of macrophages in XXXX ].

@entity35 @entity35866 @entity1506
OBJECTIVE: To study the effects of Kangai injection on the enzyme activities of macrophages and morphology of spleen and thymus from @entity35 . METHODS: Twenty four male SD @entity35 were randomly divided into two groups (n = 12), normal control group and experimental group. The @entity35 in experimental group were injected with Kangai injection at the dosage of 5 ml/kg x d for 30 days peritoneally and those in control group were injected with nomal saline at the same volume. The content of supermicro protein was assayed by BCA method, the activities of lactate dehydrogenase (LDH), glutathione peroxidase( @entity35866 ), and @entity1506 ( @entity1506 ) from alveolar macrophages(AM) and peritoneal macrophages (PM) were detected biochemically. The activities of acid phosphatase (ACP), superoxide dismutase(SOD) and succinate dehydrogenase (SDH) from AM and PM were detected by ELISA. The morphology of spleen and thymus were observed by light microscopy. RESULTS: The activities of LDH, @entity35866 and @entity1506 within AM and PM from experimental group were increased significantly compared with those of control group (P < 0.05). The activities of ACP, SOD and SDH in AM and PM from experimental group were also higher than those from control group (P < 0.05). Microscopically, there was thickening of peripheral arterial lymphatic sheath, enlargement of splenic lymphoid nodules with expended germinal center in the spleen of experimental group. There was no significant difference in the mophology of thymus between the two groups. CONCLUSION: Kangai injection may improve immune function by activating macrophages.
