A novel TBP-interacting zinc finger protein functions in early development of XXXX .

@entity1238 @entity4802 @entity72301
A zinc finger protein that interacts with @entity1238 TATA-binding protein was previously isolated by a yeast two-hybrid screen and found to serve as a transcriptional repressor. The gene was designated the @entity72301 ( @entity72301 ). Herein, @entity72301 was found to be expressed maternally. After gastrulation, the level of @entity72301 mRNA decreased significantly throughout the neurula stage. However, mRNA levels increased at stage 35 and then began to decrease at stage 48. Eventually, no @entity72301 mRNA was observed in adult tissues except in the ovary. @entity72301 mRNA was detected in the animal hemisphere during gastrulation and observed in the neural ectoderm at the neurula stage. At the tailbud stage, @entity72301 was highly expressed in the head tissues such as brain, eyes, otic vesicles, lateral line placodes, and @entity4802 , but weakly in somites. Depletion of @entity72301 in the embryos using RNA interference caused premature death at the gastrula stage or induced secondary partial axis after gastrulation. These results strongly suggest that @entity72301 is an essential transcription factor involved in the cell movement during gastrulation and the formation of the dorsal axis during early development in @entity1238 .
