Construction of orthotopic xenograft @entity19 models for XXXX @entity788 .

@entity1 @entity788 @entity5 @entity19 @entity420 @entity3 @entity7351
UNASSIGNED: Animal models are indispensable for the study of tumorigenesis and the development of anti- @entity5 drugs for @entity1 @entity788 . In the present study, two orthotopic xenograft @entity19 models were developed. @entity1 @entity1 @entity788 cells were stably labeled with red fluorescent protein ( @entity7351 ) and injected subcutaneously into @entity19 . For the @entity5 mass model, the formed subcutaneous @entity5 were cut into blocks and implanted into the pancreas of @entity19 via laparotomy. For the Matrigel    @entity5 block model, solidified Matrigel containing @entity7351 -labeled @entity1 cells was cut into blocks and implanted into the pancreas of @entity19 . A subcutaneous @entity5 xenograft model was used as a control. @entity5 growth and @entity3 were assessed using an in vivo fluorescence imaging system. Thirty-six days after implantation, all @entity19 from the two orthotopic xenograft models (n=20 per group) and 55% of the subcutaneous xenograft @entity19 (n=20) developed @entity5 . The @entity5 growth rate was significantly higher in the orthotopic models than that in the subcutaneous model (P<0.01). @entity3 to organs such as the liver was observed in the @entity5 models. Histological examination showed that the @entity5 were poorly differentiated @entity420 . In conclusion, two orthotopic xenograft @entity19 models of @entity1 @entity788 were established; these exhibited greater @entity5 growth and @entity3 than the subcutaneous xenograft @entity19 model.
