N-myc downstream regulated gene 2 overexpression reduces XXXX activities and cell invasion of @entity2 cell line in vitro.

@entity1 @entity5379 @entity1476 @entity5 @entity2 @entity16154 @entity1495 @entity2220
OBJECTIVES: N-myc downstream regulated gene 2 ( @entity16154 ) is a candidate gene for @entity5 suppression. The expression of @entity16154 is down-regulated in several @entity5 including @entity2 . The aim of this study was to explore the effect of @entity16154 overexpression on invasion, migration, and enzymatic activity of @entity1476 ( @entity5379 ( @entity1495 ) in @entity1 @entity2220 A549 cells. MATERIALS AND METHODS: A recombinant plasmid encoding green fluorescent protein (GFP)-tagged @entity16154 ( @entity16154 ) was used to overexpress GFP-tagged @entity16154 in A549 cells. The cells in the experimental group and those in the control group were transfected with @entity16154 and a control plasmid without @entity16154 (pCMV6-AC-GFP), respectively. Fluorescent microscopy and flowcytometry analysis of GFP expression were used to evaluate the cellular expression of GFP-tagged @entity16154 and the efficiency of transfection. The effects of @entity16154 expression on cell invasion and migration were evaluated using transwell filter migration assay. The gelatinase activity of secreted @entity1476 and @entity1495 was measured by gelatin zymography. RESULTS: Our results demonstrated the expression of GFP-tagged @entity16154 in the cytoplasm and nucleus of A549 cells. The findings of transwell assay showed that @entity16154 overexpression reduced migration and invasion of A549 cells compared to control cells. Gelatin zymography analyses revealed that @entity16154 overexpression decreased the gelatinase activity of secreted @entity1476 and @entity1495 . CONCLUSION: These findings suggest that @entity16154 may be a new anti-invasion factor in @entity2 that inhibits MMPs activities.
