Regulation of MHC expression in vivo. Bacterial lipopolysaccharide induces class I and II MHC products in XXXX tissues by a T cell-independent, @entity205 -sensitive mechanism.

@entity558 @entity19 @entity60 @entity205 @entity465 @entity5746 @entity526
The effect of injections of bacterial @entity558 on the expression of class I and II products of the MHC in @entity19 tissues was investigated. MHC products were assessed in tissue homogenates by radiolabeled antibody binding and in tissue sections by indirect immunoperoxidase (IIP) staining. In @entity19 given two i.p. injections of @entity558 from @entity526 or @entity5746 minnesota, there were increases in class I and II MHC products in kidney, liver, heart, lung, and pancreas. Focusing on the changes in kidney, we demonstrated that the increase in MHC expression occurred in tubules and, in the case of class I, in glomeruli. @entity558 treatment also increased the deposition of Ig in glomeruli. Expressed on a standard curve, the total kidney class I and II expression was elevated approximately 10-fold. Time course studies indicated that increased class I expression could be induced by a single @entity558 injection, whereas class II induction required a second injection. The induction was influenced by the @entity558 sensitivity of the @entity19 , being much greater in @entity558 -sensitive C3H/HeSn @entity19 than in @entity558 -resistant C3H/HeJ @entity19 . @entity558 induced class I and II Ag in @entity19 and in @entity19 with severe @entity465 , indicating that T cells were not required. Nevertheless, the effect of @entity558 was inhibitable by @entity205 and by a mAb against @entity60 indicating that @entity60 was required for the MHC induction. We conclude that @entity558 induces an increase in expression and a redistribution of MHC products in kidney and in other tissues by a T cell-independent, @entity205 -sensitive pathway. These findings are probably related to the known ability of @entity558 to mediate release of @entity60 and other cytokines.
