[Parameters of peripheral whole blood neutrophil activity in XXXX with pregnancy induced @entity101 ].

@entity1 @entity5038 @entity5029 @entity101 @entity310 @entity26 @entity4515 @entity1196 @entity1454 @entity8729 @entity9536
OBJECTIVES: Pregnancy induced @entity101 is believed to be a state of neutrophil overactivity, however all previous studies were done on isolated cells. DESIGN: To study neutrophil activity in whole blood of @entity5029 @entity1 . MATERIALS AND METHODS: Neutrophil activity was estimated without isolation, in peripheral whole blood of 23 @entity5029 @entity1 and 26 normal pregnant controls. The chemiluminescence test was performed without any stimulation and upon stimulation of neutrophils with @entity5038 , OZ and @entity4515 , before and after pre-activation with @entity310 . The results were corrected according to the haemoglobin concentration and (%) of neutrophils. The percentage of whole blood neutrophils indicating expression of selectins @entity1454 , @entity9536 , integrin @entity1196 and mean fluorescence intensity (MFI) of these molecules were studied on flow-cytometry. RESULTS: The study revealed that neutrophil chemiluminescence was not significantly higher in @entity5029 @entity1 and after correction coefficient used it was even lower in @entity5029 @entity1 . @entity310 preactivation had no influence on chemiluminescence results. Expression of @entity9536 expressed as MFI value was significantly increased while that of @entity1196 , expressed as (%) of positive cells and MFI value--decreased in @entity5029 @entity1 . CONCLUSIONS: Changes of neutrophil @entity9536 and @entity8729 expression indicate increased activity of these cells in @entity5029 @entity1 , however low production of reactive @entity26 species estimated by corrected chemiluminescence test especially after @entity310 pre-activation, indicates that this form of their reactivity is rather "exhausted" during @entity5029 .
