Effects and mechanism of XXXX from Astragalus complanatus on @entity0 growth.

@entity1 @entity0 @entity30767 @entity5 @entity19 @entity7349 @entity3088 @entity8264 @entity3 @entity1379
UNASSIGNED: @entity3088 from Astragalus complanatus R.Br (FAC) had anticancer effects on many @entity5 cells. The current study was performed to evaluate the effects of FAC on @entity1 breast cell proliferation, apoptosis, and @entity3 , as well as their active mechanism. Cell viability and growth were detected using the cell counting kit (CCK)-8 assay in vitro. Assay of FAC on induced @entity0 mortality was counted as survival time of @entity19 after breast cell line inoculation. The effect of FAC on cell invasion was investigated by an optimization assay that contains a 96-well Boyden chamber with wells precoated with BME at three different concentrations. The mechanism of its action on apoptosis and @entity3 was determined by related gene detection. Proliferation of three breast cell lines was inhibited with FAC treatment in a dose-dependent manner. Survival time of @entity19 with @entity0 cell inoculation also was prolonged with increasing FAC dose. @entity3 in FAC-treated breast cells was also significantly inhibited. Real-time polymerase chain reaction (PCR) assay demonstrated that apoptosis-related @entity1379 and @entity7349 gene expression was consistent with their phenotype change. @entity3 -related @entity8264 and @entity30767 gene expression was inversely related to FAC treatment. Western blot analysis indicated that @entity1379 and @entity8264 proteins were reduced, whereas @entity7349 and @entity30767 proteins were increased with a higher dose of FAC treatment. These data suggested that FAC has an important role in @entity0 growth and @entity3 suppression in vitro and in vivo. Its active mechanism involves promoting programmed @entity5 cell death and regulates @entity3 -related gene expression.
