Combining XXXX proteomics and in vitro cardiomyocyte phenotype testing to identify potential mediators of @entity712 with preserved ejection fraction.

@entity1 @entity712 @entity64 @entity583 @entity731 @entity460 @entity2757 @entity204 @entity65
BACKGROUND: @entity712 with ejection fraction (HFpEF) is a syndrome resulting from several co-morbidities in which specific mediators are unknown. The platelet proteome responds to disease processes. We hypothesize that the platelet proteome will change composition in @entity1 with HFpEF and may uncover mediators of the syndrome. METHODS AND RESULTS: Proteomic changes were assessed in platelets from hospitalized subjects with symptoms of HFpEF (n  =  9), the same subjects several weeks later without symptoms (n  =  7) and control subjects (n  =  8). Mass spectrometry identified 6102 proteins with five scans with peptide probabilities of >= 0.85. Of the 6102 proteins, 165 were present only in symptomatic subjects, 78 were only found in outpatient subjects and 157 proteins were unique to the control group. The @entity2757 protein was identified consistently in HFpEF samples when compared with controls. We validated the fining that plasma @entity2757 levels are increased in subjects with HFpEF (654      391) compared to controls (352      204) in an external cohort (p  =  0.002). Recombinant @entity2757 had direct effects on the electrophysiological and @entity460 handling profile in @entity1 induced pluripotent stem cell-derived cardiomyocytes. CONCLUSIONS: Platelets may harbor proteins associated with HFpEF. @entity2757 is present in the platelets of subjects with HFpEF and increased in the plasma of the same subjects. We further established a bedside-to-bench translational system that can be utilized as a secondary screen to ascertain whether the biomarkers may be an associated finding or causal to the disease process. @entity2757 has been linked with other @entity65 such as @entity731 and risk for @entity583 , @entity64 , or @entity204 . This is the first report on association of @entity2757 with HFpEF.
