XXXX candidate genes: no association with functional polymorphisms in @entity3334 gene promoters.

@entity75 @entity3334 @entity66 @entity1549
BACKGROUND: @entity66 is polygenic with a large genetic component. @entity3334 ( @entity3334 ) and @entity1549 degrade extracellular matrix; expression levels are increased in the @entity75 where tissue damage is observed. Polymorphisms associated with elevated expression ( @entity1549 -1171 allele 5A; @entity3334 -1607 2G), at 11q22.2, a region repeatedly showing evidence of linkage in @entity66 , are associated with other chronic inflammatory disorders which may share a common molecular pathology. We tested for an association between these candidate gene polymorphisms and @entity66 . METHODS: Two independent collections of 225 and 102 combined (Norwegian and Swedish) simplex families, and 160 independent healthy controls from the Norwegian Bone Marrow Donor Registry were used. Each individual was genotyped by PCR and fragment length analysis on an automated sequencer. The transmission/disequilibrium test was applied. Odds ratios were calculated employing probands or affected sibs where available, as cases versus independent controls. RESULTS: @entity3334 allele 2G did not show evidence of association in any tests undertaken. Neither did we find evidence for association of @entity1549 allele 5A, except among the combined family data: a non-significant tendency toward reduced risk was observed among males carrying @entity1549 allele 5A (40.2% transmission, Pc = 0.2). Further testing to clarify this observation did not reveal a significant association (odds ratio = 0.67 (95% confidence interval: 0.42-1.07), P = 0.08). CONCLUSIONS: We did not find significant evidence to support an association of @entity1549 allele 5A or @entity3334 allele 2G with @entity66 in Norwegian and Swedish populations.
