High performance liquid chromatographic profiling of XXXX and related indoles in body fluids and tissues of carcinoid @entity1 .

@entity1 @entity5 @entity5581 @entity2476 @entity2340 @entity750 @entity27329 @entity1130
A high performance liquid chromatographic method with quaternary gradient elution and fluorometric detection was developed for profiling of @entity2340 (TRP), @entity2476 , @entity750 ( @entity750 ) and @entity1130 ( @entity5581 ) in urine, platelet-rich plasma and ( @entity5 ) tissue of @entity1 with @entity5 . Prior to injection, urine samples were diluted and filtered. Platelet-rich plasma and tissue homogenates were prepurified by C18 solid phase extraction. Detection limits were approx. 2 pmol. Results of urinary @entity750 and @entity5581 compared favourably with those of single component analyses. No consistent diurnal variations were found for TRP, @entity750 and @entity5581 in 12-h urine samples from 15 healthy adults. Abstinence of @entity750 -rich foods reduced urinary levels of @entity750 and @entity5581 . C18 extraction of @entity27329 from protein-containing matrices was studied in platelet-rich plasma. Although time-consuming and complicated for daily routine use, the present approach offers particular advantages over single component analyses in the study of TRP metabolism in @entity1 with @entity5 .
