Liver transcriptome profiles associated with strain-specific Ehrlichia chaffeensis-induced @entity99 in SCID XXXX .

@entity1 @entity738 @entity39 @entity19 @entity5 @entity409 @entity2348 @entity20782 @entity465 @entity12443 @entity99 @entity1116
Infection of @entity1 with Ehrlichia chaffeensis, the etiologic agent of human monocytic ehrlichiosis, can cause @entity99 of various levels of severity. When the three @entity1 isolates of @entity1116 , each belonging to a different genogroup, are inoculated into severe combined @entity465 @entity19 , the order of severity of clinical signs and @entity2348 in the liver is as follows (from greatest to least severity and highest to lowest burden): strain Wakulla, followed by strain Liberty, followed by strain Arkansas. In this article, we used microarray analysis to define transcriptional profiles characteristic of the histopathological features in the @entity19 liver. Cytokine and chemokine profiles and their receptor profiles were strikingly different among the three strains of @entity1116 : gamma interferon, CCL5, CXCL1, CXCL2, CXCL7, CXCL9, interleukin 2 receptor gamma (IL2Rgamma), IL21R, CCR2, and CXCR6 were highly upregulated with strain Arkansas; and @entity5 @entity39 factor (TNF), CCL2, CCL3, CCL5, CCL6, CCL12, CCL20, CXCL2, CXCL7, CXCL9, @entity20782 , TNF receptor superfamily 9 (TNFRSF9), TNFRSF13beta, IL1R2, IL2Rgamma, IL20Rbeta, IL21R, CCR1, CCR2, and CXCR4 were highly upregulated with strain Wakulla. With strain Liberty, only @entity20782 was highly upregulated, and @entity12443 was downregulated. In livers infected with the Arkansas strain, monocytes/macrophages and NK cells were enriched in the @entity738 and an increase in NK cell marker mRNAs was detected. Livers infected with the Wakulla strain displayed infiltration of significantly more neutrophils and an increase in neutrophil marker mRNAs. Genes commonly upregulated in liver tissue infected with the three strains are other host innate immune and inflammatory response genes, including those encoding several acute-phase proteins. Genes downregulated commonly are related to host physiologic functions. The results suggest that marked modulation of host cytokine and chemokine profiles by @entity1116 strains underlies the distinct host @entity409 .
