Leukotriene synthases and the receptors induced by XXXX in the spinal cord contribute to the generation of @entity9 .

@entity71117 @entity71116 @entity19675 @entity4443 @entity565 @entity71114 @entity128 @entity71118 @entity1897 @entity71115 @entity9 @entity158 @entity35 @entity1813 @entity309 @entity26547
@entity4443 (LTs) belong to a large family of lipid mediators, termed @entity1813 , which are derived from @entity71114 and released from the cell membrane by phospholipases. LTs are involved in the pathogenesis of inflammatory diseases, such as @entity565 , @entity309 , and peripheral @entity158 . In the present study, we examined whether LTs were implicated in pathomechanism of @entity9 following @entity1897 . Using the @entity128 ( @entity128 ) model in @entity35 , we investigated the expression of LT synthases (5-lipoxygenase; 5-LO, Five lipoxygenase activating protein; @entity19675 , @entity71115 ; @entity71115 and @entity71116 ; @entity71116 ) and receptors ( @entity71117 , 2 and @entity26547 , 2) mRNAs in the @entity35 spinal cord. Semi-quantitative RT-PCR revealed that 5-LO, @entity19675 , @entity71116 , @entity71117 , and @entity26547 mRNAs increased following @entity128 , but not @entity71118 mRNAs. Using double labeling analysis of in situ hybridization with immunohistochemistry, we observed that 5-LO, @entity19675 , and @entity26547 mRNAs were expressed in spinal microglia. @entity71115 and @entity71116 mRNAs were expressed in both spinal neurons and microglia. @entity71117 mRNA was expressed in spinal neurons. The p38 mitogen-activated protein kinase inhibitor, but not MEK inhibitor, reduced the increase in 5-LO in spinal microglia. Continuous intrathecal administration of the 5-LO inhibitor or @entity71117 and @entity26547 receptor antagonists suppressed mechanical allodynia induced by @entity128 . Our findings suggest that the increase of LT synthesis in spinal microglia produced via p38 MAPK plays a role in the generation of @entity9 .
