Insulin-like growth factors (IGFs), IGF-binding proteins (IGFBPs), and proteolyzed XXXX in embryonic cavities in early @entity1 pregnancy: their potential relevance to maternal-embryonic and fetal interactions.

@entity1 @entity2301 @entity442 @entity10116 @entity5100 @entity6703 @entity2270 @entity7310 @entity734
Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) are believed to be important in fetal growth and development. In the current study, the developmental changes in the IGF and @entity2270 were examined in 23 paired samples of @entity1 @entity6703 ( @entity6703 ), extraembryonic coelomic (EEC) fluid, and @entity7310 ( @entity7310 ) between 9 and 12 weeks gestation. Levels of @entity442 were very low in @entity6703 (7 +/- 3 ng/mL) and EEC (10 +/- 3 ng/mL) compared to those in @entity7310 (237 +/- 42 ng/mL). In contrast, @entity5100 concentrations were 210 +/- 36 and 174 +/- 22 ng/mL in @entity6703 and EEC, respectively, and were approximately 25% of @entity7310 serum levels (884 +/- 122 ng/mL). There was no @entity734 on gestational age for either peptide in @entity6703 or EEC during the period of gestation examined. @entity10116 levels in @entity6703 increased about 20-fold (1.6 +/- 0.3 to 33.0 +/- 0.1 ng/mL) between 9 and 12 weeks of pregnancy, and @entity10116 levels were nearly 2 orders of magnitude higher in EEC, increasing about 100-fold (365 +/- 119 to 3014 +/- 100.0 ng/mL) by the end of the first trimester. In contrast, @entity10116 levels were low in @entity7310 (24.9 +/- 3.5 ng/mL) and showed no gestational age @entity734 . Using RIA, high levels of @entity2301 were found in EEC (2062 +/- 177 ng/mL) and @entity7310 (6590 +/- 357 ng/mL) compared to those in @entity6703 (152 +/- 24 ng/mL). Levels of @entity2301 in @entity7310 and EEC did not change significantly with gestational age, whereas an increase in @entity10116 was observed in @entity6703 after the tenth week of pregnancy. In contrast to high levels of @entity2301 in @entity7310 and EEC, determined by RIA, the 37- to 43-kilodalton @entity2301 doublet was barely detectable by Western ligand blot analysis. This discrepancy suggested the presence of an @entity2301 protease in EEC, as has been found in @entity7310 , that decreases the affinity of this BP for IGF peptides and, therefore, renders it less readily detectable by Western ligand blot analysis. Using [125I] @entity2301 as substrate, lower levels of @entity2301 protease activity were detected in EEC compared to @entity7310 , and nearly undetectable levels were found in @entity6703 . By Western immunoblotting, a smaller (28-kilodalton) immunoreactive form of @entity2301 was detected only in @entity7310 and EEC, suggesting proteolyzed @entity2301 in @entity7310 and EEC, but not in @entity6703 , during this gestational period.(ABSTRACT TRUNCATED AT 400 WORDS)
