MUC expression in XXXX and serrated @entity1586 : lack of specificity of @entity21826 .

@entity2729 @entity1127 @entity21826 @entity5 @entity5072 @entity5091 @entity5089 @entity1141 @entity2355 @entity652 @entity105 @entity1586
Previous studies have shown that @entity5072 and serrated @entity1127 of the colon show variable degrees of gastric and intestinal differentiation. MUCs are a class of approximately 20 genes that encode high-molecular-weight glycoproteins, or mucopolysaccharides, that are widely expressed in epithelial cells and show organ specificity. The role of MUC in serrated @entity105 is unknown. One previously published study suggested that expression of @entity21826 is specific for sessile serrated @entity652 / @entity1127 (SSA/Ps) and thus can be used to distinguish these lesions from @entity1127 ( @entity1127 ). However, data from our group suggest that MUC antibodies are not reliable in this differential diagnosis. The aims of this study were to systematically evaluate the expression of MUCs in serrated @entity1586 and to determine the efficacy of MUC expression in differentiating @entity1127 from SSA/Ps specifically. Routinely processed specimens from 182 serrated @entity1127 [58 @entity1127 , 46 SSA/Ps, 59 SSA/Ps with @entity1141 ( @entity2729 -D), 19 traditional serrated @entity652 , and 38 conventional tubular or tubulovillous @entity652 (CAs)] were immunohistochemically stained with @entity2355 , @entity5091 , @entity5089 , and @entity21826 , and scored for extent, intensity, and location of staining within the @entity1127 . @entity1127 were further subclassified into goblet cell type (N=18), microvesicular type (N=21), and mucin-depleted type (N=19). The data were compared between the different polyp groups and between @entity1127 from different anatomic locations in the colon. @entity2355 , @entity5091 , @entity5089 , and @entity21826 were expressed in 27%, 100%, 100%, and 72% of serrated @entity1127 overall. These antibodies were positive in 32%, 100%, 100%, and 43% of CAs. Expression levels of @entity2355 , @entity5091 , and @entity5089 were not significantly different between any of the polyp subgroups or between serrated @entity1127 and CAs. Both @entity2729 and @entity2729 -D showed a significantly higher percentage of @entity1127 that stained with @entity21826 , and a greater degree and intensity of staining for this peptide in comparison with @entity1127 . Overall, 91% of SSA/Ps and 84% of @entity2729 -Ds were positive for @entity21826 in comparison with 60% of @entity1127 (P<0.001 and P=0.02, respectively). Although @entity1127 from both the left and right colon from each polyp group showed positivity for @entity21826 , a significantly higher proportion of @entity2729 -Ds and traditional serrated @entity652 from the right colon showed @entity21826 positivity compared with those from the left. No differences were noted in @entity21826 staining between each of the 3 HP subgroups. On the basis of these data, we conclude that @entity2729 and @entity2729 -D show increased expression of @entity21826 compared with @entity1127 ; however, because of overlap in the presence, degree, and intensity of staining, use of @entity21826 to differentiate @entity1127 from @entity2729 or @entity2729 -D in individual cases is not reliable because of a lack of specificity. Differences in @entity21826 expression between right-sided and left-sided @entity1586 supports the theory that there may be biological differences in the progression of @entity5 in different portions of the colon with regard to the serrated pathway of @entity105 .
