Wispy and XXXX cooperate in the cytoplasmic polyadenylation of localized gurken mRNA.

@entity73235 @entity64566 @entity238 @entity28804
BACKGROUND: In @entity238 , the dorsal-ventral (D-V) axis of the oocyte is dependent on Gurken ( @entity28804 ) protein distribution. This is achieved through the cytoplasmic localization of @entity28804 mRNA and regulation of its translation. During mid-late stages of oogenesis, @entity28804 mRNA and protein are localized to the dorsal-anterior of the oocyte, while unlocalized @entity28804 transcripts are translationally silenced. As females carrying mutations in the gene encoding the CPEB protein @entity64566 lay ventralized eggs due to insufficient @entity28804 levels, it seemed likely that cytoplasmic polyadenylation of @entity28804 transcripts may play a role in their translational regulation. RESULTS: We have found that @entity28804 is polyadenylated throughout oogenesis, with poly(A) tails of approximately 30-50 A residues. Hyperadenylated @entity28804 transcripts, with poly(A) tails of 50-90 As, are detected in late stage egg chambers, but they fail to accumulate in oocytes deficient in @entity64566 or the poly(A) polymerase Wispy ( @entity73235 ). @entity73235 females also lay weakly ventralized eggs, demonstrating that they produce inadequate amounts of @entity28804 . Finally, unlocalized @entity28804 transcripts are also not appropriately hyperadenylated. CONCLUSIONS: Localized cytoplasmic polyadenylation of @entity28804 mRNA by @entity73235 and @entity64566 is necessary to achieve appropriate @entity28804 protein accumulation in the D/A corner of the oocyte during mid to late oogenesis. Developmental Dynamics 244:1276-1285, 2015.    2015 Wiley Periodicals, Inc.
