In-vitro and in-vivo studies of a cytotoxin from XXXX .

@entity1 @entity19 @entity294 @entity95 @entity582 @entity204 @entity5363
Studies were performed on a cytotoxin (CT) from @entity1 strains of @entity5363 isolated in Malaysia. CT was detected by cytopathic effect (CPE) on HeLa cells at titres from 8 to 32, in culture filtrates from 14 (48%) of 29 @entity1 isolates. The CPE correlated well with a quantitative 51Cr-release assay where a specific release of 54-68% was noted. CT production was lost after 5-7 subcultures. CT activity was also detected in 5 (26%) of 19 faecal filtrates from which CT-producing isolates were subsequently obtained. The mol. wt of CT was estimated by Sephadex G-50 chromatography to be greater than 30,000. In a suckling- @entity19 assay, CT consistently failed to demonstrate fluid accumulation after intragastric inoculation of culture filtrate. The Removable Intestinal Tie Adult @entity95 @entity294 (RITARD) assay was also used. @entity95 given CT-producing strains of @entity5363 developed @entity582 and severe watery mucus-containing @entity294 for the duration of the experiment with @entity204 of some animals. @entity95 given CT non-producing strains had less severe disease and none died. @entity95 given partially-purified CT had @entity294 for 3 days but none died.
