Experimental infection of embryonated eggs of XXXX with Besnoitia caprae.

@entity32 @entity548 @entity39 @entity7843 @entity36323 @entity450 @entity1573 @entity6675 @entity401 @entity912
Knowledge on parasites of the genus Besnoitia, especially Besnoitia caprae, is sparse. Besnoitia caprae, an obligate intracellular protozoan parasite belonging to the phylum Apicomplexa, is the causative agent of @entity36323 @entity7843 . This experiment was conducted to determine the infectivity of the bradyzoites and the resultant histopathological lesions after inoculation of B. caprae bradyzoites in the embryonated egg. Eight groups, each having six embryonated eggs, were assigned in this experiment. Seven groups were inoculated with different doses of B. caprae bradyzoite inoculums (1x10(3), 1x10(4), 1x10(5), 1x10(6), 5x10(6), 1x10(7) and 2x10(7)) via the @entity6675 route. The 8th group was considered as control. The embryos inoculated with higher doses showed mortality between 14 and 21 days of incubation (5-12 days post-infection). Those embryos that received lower doses were hatched on day 21 of incubation; however, they presented loss of feathers and @entity912 and showed @entity450 in the skin of the feet regions. Histopathological sections of the skin revealed the presence of @entity548 , @entity450 and @entity32 responses. Some of the @entity1573 were euthanized after 50 days postinfection (DPI) and histopathological examination of their tissues revealed @entity548 and @entity39 with the presence of mononuclear cells infiltration in the liver and heart with @entity401 . It seems that the embryonated eggs could be a useful model to study the parasite's biology.
