Effects of Brassica vegetable juice on the induction of apoptosis and aberrant crypt foci in XXXX colonic mucosal crypts in vivo.

@entity17645 @entity18399 @entity25961 @entity4498 @entity8708 @entity24150 @entity14 @entity35 @entity599 @entity105 @entity204 @entity9083
@entity24150 are @entity599 -containing glycosides found in the Brassica vegetables. Their breakdown products include @entity9083 , which are produced following exposure to the endogenous plant enzyme myrosinase. Isothiocyanates are pungent, biologically active compounds that suppress @entity105 in vivo, and induce apoptosis in vitro. We have shown previously that oral administration of the isolated glucosinolate @entity25961 induces apoptosis, and suppresses aberrant crypt foci in the colonic mucosa of @entity35 treated previously with @entity8708 ( @entity18399 ). In this study we explored the effects of both raw and thermally processed Brussels sprout tissue on the modulation of crypt cell apoptosis and @entity4498 , and the frequency of aberrant crypt foci in the colon. Freeze-dried raw and microwave-cooked Brussels sprouts contained high levels of intact glucosinolates, but they were absent from freshly prepared sprout juice. Oral administration of uncooked Brussels sprouts, whether as a juice, or as a freeze-dried powder, was associated with significantly enhanced levels of apoptosis and reduced @entity4498 in the colonic crypts. However, this effect was confined to @entity35 previously injected (48 h) with @entity18399 , in which levels of apoptosis and @entity4498 following DNA damage were already high. There was no effect of treatment in control animals. There was also little evidence of these effects when intact glucosinolates were administered in blanched sprout tissue, which lacked active myrosinase. We conclude that @entity17645 breakdown products derived from Brassica vegetables can exert a profound effect on the balance of colorectal cell proliferation and @entity204 in an animal model of @entity14 .
