Dual function of the hemagglutinin H5 fused to @entity1573 XXXX in a potential strategy of DIVA against avian influenza disease: preliminary study.

@entity71615 @entity1573 @entity1800 @entity71614 @entity526
UNASSIGNED: In this study we demonstrated that the vaccine candidate against avian influenza virus H5N1 based on the hemagglutinin H5 (HA) fused to the @entity1573 @entity71614 ( @entity71615 ) can also be used for differentiating infected from vaccinated animals (DIVA). As the strategy of DIVA requires at least two proteins, we obtained a variant of the nucleoprotein (NP49-375) in @entity526 . After its purification by IMAC, the competence of the proteins NP49-375 and @entity71615 as coating antigens in indirect ELISA assays were tested by using the sera of @entity1573 immunized with the proteins HA and @entity71615 and the reference sera from several avian influenza subtypes. Together with these sera, the sera from different species of birds and the sera of @entity1573 infected with other avian @entity1800 were analyzed by competition ELISA assays coated with the proteins NP49-375 and @entity71615 . The results showed that the segment @entity71614 in the chimeric protein @entity71615 did not interfere with the recognition of the molecule HA by its specific antibodies. Also, we observed variable detection levels when the reference sera were analyzed in the ELISA plates coated with the protein NP49-375. Moreover, only the antibodies of the reference serum subtype H5 were detected in the ELISA plates coated with the protein @entity71615 . The competition ELISA assays showed percentages of inhibition of 88-91% for the positives sera and less than 20% for the negative sera. We fixed the cut-off value of these assays at 25%. No antibody detection was observed in the sera from different species of birds or the sera of @entity1573 infected with other avian @entity1800 . This study supported the fact that the ELISA assays using the proteins NP49-375 and @entity71615 could be valuable tools for avian influenza surveillance and as a strategy of DIVA for counteracting the highly pathogenic avian influenza virus H5N1 outbreaks.
