Molecular Analysis of the Cyanobacterial Community in Gastric Contents of Egrets with Symptoms of XXXX .

@entity12219 @entity204 @entity63486
Many deaths of wild birds that have drunk water contaminated with hepatotoxic @entity12219 -producing cyanobacteria have been reported. A mass @entity204 of egrets and herons with @entity63486 were found at the agricultural reservoir occurring cyanobacterial waterblooms. This study aimed to verify a hypothesis that the egrets and herons which died in the reservoir drink @entity12219 -producing cyanobacteria and @entity12219 involves in the cause of @entity204 as well as the symptoms of @entity63486 . The cyanobacterial community in gastric contents of egrets and herons that died from @entity63486 was assessed using cyanobacterial 16S rRNA-based terminal-restriction fragment length polymorphism (T-RFLP) profiling and a cyanobacterial 16S rRNA-based clone library analysis. In addition, PCR amplification of the mcyB-C region and the mcyG gene, involved in @entity12219 biosynthesis, was examined. The cyanobacterial community in the gastric contents of two birds showed a simplistic composition. A comparison of cyanobacterial T-RFLP profiling and cloned sequences suggested that the genus Microcystis predominated in both samples of egrets died. Although we confirmed that two egrets which died in the reservoir have taken in cyanobacterial waterblooms containing the genus Microcystis, no mcy gene was detected in both samples according to the mcy gene-based PCR analysis. This study is the first to show the profiling and traceability of a cyanobacterial community in the gastric contents of wild birds by molecular analysis. Additionally, we consider causing symptoms of @entity63486 in the dead egrets.
