XXXX : detection of abnormal cells in peritumoral tissue. A cytophotometry and immunocytochemistry study.

@entity5 @entity1 @entity1358 @entity703
To spare organ function, partial resection of early diagnosed @entity1358 ( @entity1358 ) is applied for well-localized and small-volume @entity1358 with increasing frequency, although recurrence of the @entity5 in the same kidney is occasionally observed. The aim of the present study was to establish objective prognostic parameters that would allow the selection of @entity5 suitable for an organsaving procedure. Of the 160 @entity1 undergoing a radical nephrectomy, 67 were included in this study. In 7/45 @entity1 with lymph-node dissection (15.6%), clinical staging revealed a false-negative lymph-node status. By means of conventional histopathology, multifocality could be demonstrated in 2/67 @entity1 (3%); in 1/67 @entity1 (1.5%), the ipsilateral adrenal gland was unexpectantly @entity5 -involved. Both @entity5 tissue and normal peritumoral tissue were examined for the presence of premalignant and @entity5 cells on the basis of DNA ploidy and of the expression of the @entity5 -associated G250 antigen, which is specifically expressed at the surface of @entity703 cells. In 40/67 (59.1%) peritumoral tissue specimens, cells with an abnormal DNA content could be observed using automated image analysis. In 12/67 cases (18%), cells obtained from peritumoral tissue also showed an aneuploid DNA histogram; 4/67 (6%) had a @entity5 -correlated DNA ploidy. Additionally, 38/67 (56.9%) of these tissues, histopathologically classified as normal, contained cells expressing the G250 antigen. These observations were independent of the stage or histological grade of the @entity5 . These data indicate that classic pathological parameters for @entity5 staging are insufficient for the detection of multifocality, occurring in more than 15% of cases.(ABSTRACT TRUNCATED AT 250 WORDS)
