Effect of a single dose of @entity3215 on hepatic cell proliferation and the DNA binding activity of NF-kappaB and @entity19505 in XXXX .

@entity5241 @entity4336 @entity3215 @entity19505 @entity12053 @entity35 @entity157 @entity28168
@entity3215 ( @entity3215 ) are environmental pollutants that, because of their persistence and biomagnification, raise concerns about the health consequences of long-term exposure. @entity5241 mixtures induce @entity157 in rodents, but the mechanism of their promoting activity is not clear. Previous studies have shown that oxidative stress occurs after @entity5241 administration, with the induction of lipid peroxidation and oxidative DNA damage, which may contribute to their promoting activity. In this study, we examined whether the oxidative stress-sensitive transcription factors NF-kappaB or @entity19505 were activated by @entity3215 in the liver. Male @entity35 were injected i.p. with @entity4336 oil, @entity12053 ( @entity12053 , 30, 150, or 300 micromol/kg), @entity28168 ( @entity28168 , 30, 150, or 300 micromol/kg), or both @entity3215 (each 30 or 150 micromol/kg). @entity35 were euthanized 2, 6, or 24 h, or 2, 6, and 10 d after the @entity5241 injection. Electrophoretic mobility shift assays (EMSAs) were performed to determine NF-kappaB and @entity19505 DNA binding activities. The highest NF-kappaB DNA binding activity was observed in @entity35 receiving higher doses of @entity12053 (150 and 300 micromol/kg), with peak activation occurring 2 d after injection. @entity19505 activation was not detected at any timepoint. Hepatocyte proliferation, as measured by the labeling index, was increased only in groups receiving the highest dose of @entity12053 or the combination of two @entity3215 (150 micromol/kg each) at day 2, and not by any other @entity5241 treatment at any timepoint. These results show that @entity12053 , but not @entity28168 , can induce hepatocyte proliferation and hepatic NF-kappaB activation after a single dose.
