X-ray irradiation and Rho-kinase inhibitor additively induce invasiveness of the cells of the @entity788 , which exhibits XXXX .

@entity1 @entity788 @entity1476 @entity5 @entity9067 @entity53127 @entity361 @entity1319
@entity5 cells can migrate and invade tissue by two modes of motility: mesenchymal and amoeboid. X-ray or y-ray irradiation increases the invasiveness of @entity5 cells with mesenchymal motility through the induction of matrix metalloproteinases (MMP), and this increase is suppressed by MMP inhibitors (MMPI). However, the effects of X-ray or y-ray irradiation on the invasiveness of @entity5 cells with @entity1319 remain unclear. We investigated the effect of irradiation on @entity1319 by using cells of the @entity1 @entity788 , which exhibits both modes of motility. The X-ray-induced invasiveness of MIAPaCa-2 cells was associated with the upregulation of @entity1476 at both the RNA and protein levels and was inhibited by @entity53127 treatment. Amoeboid-mesenchymal transition was slightly induced after irradiation. The MMPI treatment caused @entity361 without significant increase in invasiveness, while the @entity9067 ( @entity9067 ) stimulated amoeboid-mesenchymal transition and enhanced invasiveness under both non-irradiated and irradiated conditions. This @entity9067 -induced transition was accompanied by the upregulation of @entity1476 mRNA and protein. Exposure to both irradiation and @entity9067 further enhanced @entity1476 expression and had an additive effect on the invasiveness of MIAPaCa-2 cells. Additionally, exposure to MMPI led to significant suppression of both radiation-induced and the basal invasiveness of MIAPaCa-2 cells. This suggests that @entity9067 treatment, especially with concomitant X-ray irradiation, can induce invasion of @entity5 cells and should be used only for certain types of @entity5 cells. Simultaneous use of inhibitors, @entity9067 and MMPI may be effective in suppressing invasiveness under both X-ray-irradiated and non-irradiated conditions.
