Megakaryocytes from @entity1 with @entity453 express the inducible XXXX synthase.

@entity1 @entity7792 @entity9554 @entity842 @entity453 @entity141 @entity731 @entity3623
Endothelial and platelet generation of @entity842 (NO) plays an important role in the regulation of hemostasis. Alterations in NO biosynthesis are described in @entity731 . We have investigated the NO pathway in megakaryocytes and platelets from @entity1 with @entity731 and age-matched control subjects. Megakaryocytes and platelets were isolated from @entity1 with severe @entity453 (n = 19) and normal coronary arteries (n = 9) as demonstrated by selective angiography. Constitutive ( @entity141 -dependent) and inducible ( @entity141 -independent) NO synthase ( @entity9554 and @entity3623 , respectively) activities were measured by using the @entity7792 assay and by immunostaining techniques using an anti-peptide antibody to @entity3623 . Megakaryocytes from @entity1 with @entity731 expressed significantly greater amounts of @entity3623 (1.28 +/- 0.46 pmol @entity7792 .mg-1.min-1) than @entity9554 (0.29 +/- 0.40 pmol.mg-1.min-1). In contrast, megakaryocytes from @entity1 with normal coronary arteries expressed significantly more @entity9554 (1.48 +/- 0.23 pmol.mg-1.min-1) than @entity3623 (0.49 +/- 0.40 pmol.mg-1.min-1). Platelets isolated from both groups showed no significant difference in @entity9554 expression, and no @entity3623 was seen in either group. Immunostaining confirmed the presence of the @entity3623 in megakaryocytes. These results suggest there is a link between the expression of @entity3623 in the megakaryocyte and @entity731 .
