Diversity of XXXX isolates from woodland rhizosphere environments.

@entity1 @entity9179 @entity6402 @entity15006
AIMS: Determination of genetic diversity among UK @entity6402 isolates from various environmental niches, principally woodland tree rhizospheres and onions. METHODS AND RESULTS: Genus determination was made using polymerase chain reaction (PCR) amplification and @entity15006 profiling. Genetic diversity was investigated by repetitive sequence genetic PCR fingerprinting. Several onion isolates were similar to clinical isolates but others were diverse. Some environmental isolates were possibly synonymous with @entity6402 and @entity9179 but most from woodland rhizospheres were distinct and clustered together. The 16S rRNA genes of representatives from these clusters were PCR amplified, sequenced and phylogenetically compared with all known @entity9179 and related species. This revealed that the rhizospheric isolates had closest affinity with @entity9179 spp. with known bioremediative and biocontrol capabilities and were unrelated to taxa comprising plant or @entity1 pathogenic strains. CONCLUSIONS: All of the analyses investigated revealed that environmental and onion isolates of @entity6402 complex bacteria are genetically diverse but that woodland rhizospheric isolates are related to each other and unrelated to plant or @entity1 pathogenic strains. SIGNIFICANCE AND IMPACT OF THE STUDY: Woodland rhizospheric isolates of @entity6402 are potentially good candidates for use in bioremediation and biocontrol, as they appear distinct from plant or @entity1 pathogenic strains.
