A Rapid, Selective and Sensitive UPLC-MS/MS Method for Quantification of Nomilin in XXXX Plasma and Its Application in a Pharmacokinetic Study.

@entity1046 @entity1626 @entity1172 @entity35 @entity3092 @entity46145 @entity5189
UNASSIGNED: Nomilin is a potential anticancer agent. In this study, a rapid, sensitive, and simple ultra-performance liquid chromatography with tandem mass spectrometry methodology was established and validated to quantify nomilin in @entity35 plasma. Plasma samples were prepared through liquid-liquid extraction using @entity1046 . @entity1172 was performed using an Acquity HSS T3 column. @entity1626 and water containing 0.1% (v/v) @entity5189 were used as mobile phases at a flow rate of 0.3   mL/min. Nomilin and @entity3092 (internal standard) were detected and quantified via a triple quadrupole tandem mass spectrometer in the positive ion mode with multiple reaction monitoring. Tandem mass spectrometry detection was performed by monitoring the fragmentations of m/z 515.3  >  m/z 161.0 and m/z 303.2  >  m/z 153.1 of nomilin and @entity3092 , respectively. Good linearity (R(2)   >   0.996) was observed in the concentration range of 1   ng/mL to 500   ng/mL with a lower limit of quantification of 1   ng/mL for nomilin. The average extraction recoveries of nomilin and @entity3092 were >   82.3% and 82.0%, respectively. Intra- and interday precisions were less than 15% and accuracy ranged from 85.0% to 90.1%. Indeed, the proposed method was successfully applied to analyze the pharmacokinetics of nomilin after 3 and 50   mg/kg @entity46145 were administered to @entity35 via intravenous and oral routes, respectively.
