Effect of b-amyloid (25-35) on mitochondrial function and expression of mitochondrial permeability transition pore proteins in XXXX hippocampal neurons.

@entity5212 @entity1708 @entity35 @entity1249 @entity141 @entity32049 @entity2383
The aim of this study was to assess the effect of the b-amyloid fragment Ab(25-35) on mitochondrial structure and function and on the expression of proteins associated with the mitochondrial permeability transition pore ( @entity5212 ) in @entity35 hippocampal neurons. Ninety clean-grade @entity35 were randomly assigned to six groups (n   =   15 per group). Ab(25-35) (1, 5, or 10     g/ @entity35 ) was injected into hippocampal area @entity1249 . Normal saline was injected as a control. The effect of Ab(25-35) injection on hippocampal structure was assessed by transmission electron microscopy. @entity141 -ATPase activity, [ @entity141 ](i) , and mitochondrial membrane potential were measured. The expression of genes associated with the @entity5212 , including the voltage-dependent anion channel (VDAC), @entity1708 translocator (ANT), and @entity32049 ( @entity32049 ), were evaluated. Results showed that Ab(25-35) injection damaged the mitochondrial structure of hippocampal neurons, decreased @entity141 -ATPase activity and mitochondrial membrane potential, and increased [ @entity141 ](i) . The expression levels for VDAC, ANT, and @entity32049 in all groups were significantly (P   <   0.05) higher than those in the normal control group after Ab(25-35) injection. These results indicate that Ab(25-35) damages mitochondria in @entity35 hippocampal neurons and effects mitochondrial dysfunction, as well as increasing the expression of genes associated with the @entity5212 . @entity2383 may result in increased @entity5212 gene expression, leading to neurodegenerative effects.
