Status of epigenetic chromatin modification enzymes and XXXX risk in northeast Indian population.

@entity1 @entity286 @entity2991 @entity5 @entity32656 @entity27563 @entity1721 @entity41845 @entity70647 @entity418 @entity18097 @entity285
@entity418 incidence is reported in high frequency in northeast India. The etiology is different from other population at India due to wide variations in dietary habits or nutritional factors, @entity286 /betel quid chewing and @entity285 habits. Since DNA methylation, histone modification and miRNA-mediated epigenetic processes alter the gene expression, the involvement of these processes might be useful to find out epigenetic markers of @entity418 risk in northeast Indian population. The present investigation was aimed to carryout differential expression profiling of chromatin modification enzymes in @entity5 and normal tissue collected from @entity1721 ( @entity1721 ) @entity1 . Differential mRNA expression profiling and their validation was done by quantitative real time PCR and tissue microarray respectively. Univariate and multiple logistic regression analysis were used to analyze the epidemiological data. mRNA expression data was analyzed by Student t-test. Fisher exact test was used for tissue microarray data analysis. Higher expression of enzymes regulating methylation ( @entity41845 and @entity32656 ) and acetylation ( @entity70647 , @entity2991 , @entity27563 and @entity18097 ) of histone was found associated with @entity1721 risk. Tissue microarray done in independent cohort of 75 @entity1 revealed higher nuclear protein expression of @entity2991 and @entity32656 in @entity5 similar to mRNA expression. Expression status of @entity32656 and @entity2991 was found declined as we move from low grade to high grade @entity5 . Betel nut chewing, @entity285 drinking and dried fish intake were significantly associated with increased risk of @entity418 among the study subject. Study suggests the association of @entity32656 and @entity2991 with @entity418 risk and its involvement in the transition process of low to high grade @entity5 formation. The study exposes the differential status of chromatin modification enzymes between @entity5 and normal tissue and points out that relaxed state of chromatin facilitates more transcriptionally active genome in esophageal carcinogenesis.
