[Small interfering RNA inhibits cell proliferation in XXXX cell lines highly expressing RegIa].

@entity1 @entity98 @entity15206 @entity356
OBJECTIVE: To investigate the effect of small interfering RNA on cell proliferation and apoptosis in @entity356 cell lines with high expression of RegIa. METHODS: Total RNA was isolated from six @entity356 cell lines,and the expression of RegI a mRNA was detected by RT-PCR. RegI a RNAi expression vector was constructed and stably transfected into @entity1 and @entity15206 cells with high RegI a expression, empty-vector was used as control. RegI a mRNA and protein expression was measured by RT-PCR and Western blot respectively in stable transfected cell lines. Cell proliferation and apoptosis were detected with @entity98 assay and flow cytometry. RESULT: RT-PCR results indicated that RegI a mRNA expression was significantly inhibited by RNAi in both cell lines compared with empty-vector. Western blot results showed that RegIa protein was down-regulated to (44    4)% and (25    4)% respectively in @entity1 and @entity15206 cells compared to empty-vector. MTT results showed that cell growth was significantly inhibited in @entity1 and @entity15206 cells. The apoptosis rate in @entity1 and @entity15206 cells was remarkable increased compared to that of empty-vector (12.96    0.50)% compared with (3.99    0.30)% and (11.59    1.10)% compared with (4.22    0.40)% (P < 0.05). CONCLUSION: Small interfering RNA of RegI a gene can efficiently down-regulate RegI a expression in @entity1 and @entity15206 cell lines, and further inhibit cell growth and induce cell apoptosis.
