The antimicrobial activity of free and immobilized @entity10069 in nanoparticles of XXXX .

@entity4625 @entity10069 @entity1 @entity137 @entity27704 @entity794 @entity15745 @entity5132 @entity3677 @entity2142 @entity526
BACKGROUND: Several cationic polymers exhibit a useful antimicrobial property, however the structure-activity relationship still requires a more complete investigation. The main objective of this work is the comparison between the antimicrobial activity and @entity137 of free and immobilized @entity10069 ( @entity27704 ) in biocompatible @entity4625 ( @entity4625 ) nanoparticles (NPs). RESULTS: NPs synthesis by emulsion polymerization is performed over a range of [ @entity27704 ] at two @entity3677 ( @entity15745 ) concentrations. The @entity4625 / @entity27704 dispersions are characterized by dynamic light-scattering for sizing, polydispersity and zeta-potential analysis, scanning electron microscopy (SEM), plating plus colony forming unities (CFU) counting for determination of the minimal microbicidal concentrations (MMC) against @entity526 , @entity794 and @entity5132 and @entity2142 evaluation against @entity1 erythrocytes. There is a high colloidal stability for the cationic @entity4625 / @entity27704 NPs over a range of [ @entity27704 ]. NPs diverse antimicrobial activity against the microorganisms reduces cell viability by eight-logs ( @entity526 ), seven-logs ( @entity794 ) or two-logs ( @entity5132 ). The NPs completely kill @entity526 over a range of [ @entity27704 ] that are innocuous to the erythrocytes. Free @entity27704 antimicrobial activity is higher than the one observed for @entity27704 in the NPs. There is no @entity27704 induced- @entity2142 at the MMC in contrast to the hemolytic effect of immobilized @entity27704 in the NPs. @entity2142 is higher than 15  % for immobilized @entity27704 at the MMC for @entity794 and @entity5132 . CONCLUSIONS: The mobility of the cationic antimicrobial polymer @entity27704 determines its access to the inner layers of the cell wall and the cell membrane, the major sites of @entity27704 antimicrobial action. @entity27704 freedom does matter for determining the antimicrobial activity at low @entity27704 concentrations and absence of @entity2142 .
