Role of epithelial-mesenchymal transition markers in triple-negative XXXX .

@entity1 @entity0 @entity5 @entity5962 @entity19099 @entity2533
UNASSIGNED: @entity0 ( @entity0 ) are a heterogeneous group of @entity0 that are often associated with adverse pathological characteristics, poorer clinical outcomes and lack of targeted therapeutic options. Epithelial-mesenchymal transition, which plays a crucial @entity5 development and progression, is characterised by a transition from epithelial to mesenchymal phenotype and loss of proteins involved in maintaining cell junctions. We aimed to correlate protein expression of @entity5962 , @entity19099 and @entity2533 ( @entity2533 ) with clinicopathological parameters and survivals of a series of @entity1 with TNBC. The study cohort comprised 767 @entity0 diagnosed at the Department of Pathology, Singapore General Hospital from 1994 to 2012. Immunohistochemistry was performed on sections cut from tissue microarrays using the polymeric method. Staining intensity and percentage of positive @entity5 cells were evaluated and correlated with clinicopathological findings and clinical outcomes. Loss of @entity5962 expression, @entity19099 positivity, cytoplasmic and nuclear expression of @entity2533 were observed in 265 (35.2  %), 241 (32.0  %), 272 (36.2  %) and 262 (34.8  %) @entity5 , respectively. Histological grade significantly correlated with  @entity19099 positivity (P < 0.001) and loss of membranous @entity5962 expression (P  =  0.003). Nuclear expression of @entity2533 was inversely correlated with histological grade (P  =  0.010). Median follow-up was 73  months, with a maximum of 236  months. Despite a graphical curve for earlier recurrence in @entity1 with @entity5 harbouring a combinational phenotype of loss of membranous @entity5962 and positive @entity19099 expression, there was no statistical significance. Similarly for @entity1 with @entity5 expressing cytoplasmic @entity2533 , graphical representation showed poorer metastasis-free survival but without statistical significance. Loss of membranous @entity5962 and positive @entity19099 expression are significantly associated with high-grade @entity0 . More work is needed to improve understanding of the role of @entity2533 in TNBC.
