Calea zacatechichi @entity1044 extract exhibits antidiarrheal and antinociceptive effects in XXXX models mimicking @entity1744 .

@entity1044 @entity19 @entity1042 @entity294 @entity344 @entity17058 @entity158 @entity1744
UNASSIGNED: Calea zacatechichi Schltdl. (Asteraceae alt. Compositae) is a Mexican plant commonly used in folk medicine to treat @entity344 . The objective of this study is to characterize the effect of C. zacatechichi extracts in @entity19 models mimicking the symptoms of @entity1744 ( @entity1744 ). Powdered C. zacatechichi herb (leaves, stems, and flowers) was extracted with @entity1042 . Methanolic extract was filtered and evaporated giving methanolic fraction. The residue was extracted with @entity1044 ( @entity17058 ). Methanolic and @entity17058 (200  mg/kg, per os) extracts were screened for their effect on GI motility in several in vitro tests, and the antidiarrheal and antinociceptive effects were assessed using @entity19 models. The influence of the @entity17058 extract on motoric parameters and exploratory behaviors was also assessed. Finally, the composition of C. zacatechichi @entity17058 extract was qualitatively analyzed using liquid chromatography-mass spectrometry (LC-MS) method. C. zacatechichi @entity17058 extract significantly inhibited the contractility of @entity19 colon in vitro (IC50  =  17      2   g/ml). Administration of the @entity17058 extract in vivo (200  mg/kg, per os) significantly prolonged the time of whole GI transit (46      1 vs. 117      27  min for control and @entity17058 -treated animals, respectively; P  =  0.0023), inhibited hypermotility, and reduced @entity158 in @entity19 models mimicking functional @entity344 . Our findings suggest that constituents of the C. zacatechichi @entity17058 extract exhibit antidiarrheal and analgesic activity. The extract may thus become an attractive material for isolation of compounds that may be used as a supplementary treatment for @entity158 and @entity294 associated with @entity1744 in the future.
