The isolation of two proteins, glycoprotein I and a trypsin inhibitor, from the seeds of XXXX ( @entity11297 ).

@entity46468 @entity6122 @entity11297 @entity535 @entity4583 @entity1735 @entity9750 @entity3953 @entity3913 @entity1434 @entity4857 @entity3363
1. The isolation of two proteins from the seeds of @entity11297 is described. 2. The individual steps in the purification procedure included: extraction of the seeds at pH9.0, dialysis, first against pH9.0 and then against pH5.0 buffers, high-voltage electrophoresis of the proteins soluble at pH5.0 and chromatography on Sephadex G-200, Sephadex G-75 and DEAE-Sephadex columns. 3. Of the two proteins isolated, the first and larger component was a glycoprotein and its @entity1735 part was mainly composed of @entity3913 and @entity46468 together with smaller amounts of @entity4583 , @entity6122 and @entity3953 . 4. The second protein component isolated was a trypsin inhibitor and was almost entirely devoid of @entity3363 but contained a firmly bound pinkish-blue pigment. 5. The @entity535 composition of the two proteins was determined. 6. The glycoprotein contained very little if any cyst(e)ine but was relatively rich in @entity9750 , whereas the trypsin inhibitor had an unusually high @entity1434 content (nearly 15%) but was relatively poor in @entity4857 and in @entity9750 .
