Inhibition of XXXX might reverse @entity900 resistance of A549/ @entity1149 cell line.

@entity1 @entity15353 @entity1262 @entity11833 @entity588 @entity5 @entity3514 @entity1149 @entity900 @entity72192 @entity98 @entity2454 @entity11092
UNASSIGNED: We aimed to explore the possible mechanism of @entity72192 (miR-196a) inhibition and reversion of drug resistance to @entity900 ( @entity15353 ) of the A549/ @entity1149 ( @entity1149 ) cell line. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect expression differences of miR-196a in the drug-resistant A549/ @entity15353 NLCLC cell line and the parental A549 cell line, and expressions of miR-196a in the A549/ @entity15353 NLCLC cell line transfected with miR-196a inhibitor (anti-miR-196a group) and the miR-196a negative control (miR-NC) group and blank group (without transfection). @entity2454 ( @entity98 ) test was applied in examining the cell viability of A549/ @entity15353 cell line before and after transfection. Clonogenic assay was used to detect cell proliferation ability. Flow cytometry was applied in detecting apoptosis rate of assayed @entity5 cell and @entity3514 changes in cells. Western blot was applied in detecting proteins of drug-resistant related gene in A549/ @entity15353 cell line. Significantly higher expression of miR-196a was detected in the drug-resistant A549/ @entity15353 cell line than that in the parental A549 cell line (P   <   0.05). However, miR-196a expression in the anti-miR-196a group decreased obviously compared to that in the blank group and the miR-NC group (both P   <   0.05); The value of IC50 in the anti-miR-196a group showed remarkably lower than that in the blank group and the miR-NC group (both P   <   0.05); @entity3514 absorbing ability in the anti-miR-196a group increased 2.51 times and 2.49 times respectively compared to that in the blank group and the miR-NC group (both P   <   0.05). No statistical differences in the apoptosis rate of A549/ @entity15353 cell line in the early stage were found among the three groups (all P   >   0.05), but the late-stage apoptosis rate in the anti-miR-196a group was significantly higher than that in the blank group and the miR-NC group (both P   <   0.05); The expressions of @entity1 @entity1262 ( @entity1262 ), @entity11092 ( @entity11092 ), excision repair cross-complementation 1 ( @entity11833 ), survivin, and @entity588 ( @entity588 ) decreased significantly while RhoE increased significantly in the anti-miR-196a group than the blank group and the miR-NC group (all P   <   0.05). Inhibition of miR-196a could reverse @entity900 resistance of A549/ @entity15353 cell lines, which might relate with inhibition of drug efflux, down-regulation of drug-resistant protein expression, cell apoptosis, and cell proliferation suppression.
