Comparison of media used for XXXX in vitro fertilization and embryo transfer programs--a new method of serum preparation.

@entity1 @entity36312 @entity19 @entity3157 @entity16557
Two-cell ICR- @entity19 embryos were cultured to compare media used for @entity1 in vitro fertilization and embryo transfer (IVF-ET) programs. When delayed centrifuged (DC, a general method of serum preparation) adult @entity16557 ( @entity16557 ) was used as a medium supplement, Whitten's modified Krebs-Ringer- @entity3157 solution (m-KRB) was significantly better in both rate of development to blastocysts (D. R. to b1) and hatching rate (H.R.) than modified Ham's F-10 medium (m- @entity36312 ), irrespective of the commercial source of the @entity36312 powder. As for medium supplements when m-KRB was used as the medium base, immediately cooled and centrifuged (ICC, a new method of serum preparation) @entity16557 was significantly better in D.R. to bl. and H.R. than either DC- @entity16557 or DC-cord blood serum (CBS). There was no significant difference between DC- @entity16557 and DC-CBS. Moreover, ICC- @entity16557 gave a much higher H.R. than either bovine serum albumin (BSA) or human serum albumin (HSA), although D.R. to bl. was similar in all three. There was no significant difference between BSA and HSA. In clinical assessment of a @entity1 IVF-ET program, both the fertilization rate and the cleavage rate were higher in m-KRB containing ICC-patient's serum (PS) than in m- @entity36312 containing DC-PS, although the differences were not statistically significant.
