Cell apoptosis and proliferation inhibition of XXXX induced by sub-threshold focused ultrasound (FUS).

@entity1 @entity3123 @entity788 @entity39 @entity19 @entity5 @entity1711 @entity1977 @entity1710
OBJECTIVE: To evaluate the effects of sub-threshold focused ultrasound (FUS) sonication on the @entity788 cell. MATERIALS AND METHODS: The @entity1 @entity3123 cell line PaTu 8988t suspension and @entity3123 xenograft in @entity19 were sonicated by FUS using sub-threshold doses. The temperature at the focus was controlled at below 60  C. The cell apoptosis in vitro was tested by flow cytometer at 3, 6, 12, 24 and 48h after FUS sonication. Colony formation was used to evaluate the cell growth inhibition of FUS in vitro. The @entity5 volume of the xenograft was measured before and after FUS sonication. Then the slides of the @entity5 were under @entity1710 - @entity1711 (H_E) staining and TdT-mediated @entity1977 nick end labeling (TUNEL) to evaluate the effect of FUS on @entity3123 xenograft in vivo. RESULTS: The maximum cell suspension temperature of the FUS sonication group was 55.8  2.17  C. The cell apoptosis rate peaked at 24h after FUS sonication, the differences between the FUS sonication group and control group were statistically significant (P<0.05). Colony formation rates were 50.40  3.81% for the control group and 26.82  2.88% for FUS sonication group (P<0.05). For the xenografts in @entity19 , the mean @entity5 volumes of the control group and the FUS sonication group 15 days after sonication were 1746.58  312.77mm(3) and 1085.23  217.13mm(3) (P<0.05). H_E staining and TUNEL assay showed both @entity39 and apoptotic cells. CONCLUSION: Sub-threshold FUS sonication could induce cell apoptosis and inhibit the proliferation of @entity788 cells.
