Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during XXXX Liver Regeneration.

@entity29973 @entity43956 @entity73262 @entity12072 @entity73263 @entity17985 @entity1975 @entity12073 @entity51611 @entity35 @entity12075 @entity18517 @entity18715
OBJECTIVE: To investigate the transdifferentiation relationship between eight types of liver cell during @entity35 liver regeneration (LR). MATERIALS AND METHODS: 114 healthy Sprague-Dawley (SD) @entity35 were used in this experimental study. Eight types of liver cell were isolated and purified with percoll density gradient centrifugation and immunomagentic bead methods. Marker genes for eight types of cell were obtained by retrieving the relevant references and databases. Expression changes of markers for each cell of the eight cell types were measured using microarray. The relationships between the expression profiles of marker genes and transdifferentiation among liver cells were analyzed using bioinformatics. Liver cell transdifferentiation was predicted by comparing expression profiles of marker genes in different liver cells. RESULTS: During LR hepatocytes (HCs) not only express hepatic oval cells (HOC) markers (including @entity18517 , @entity12072 and @entity73262 ), but also express biliary epithelial cell (BEC) markers (including @entity43956 and @entity29973 ); BECs express both HOC markers (including @entity73263 , @entity1975 and @entity18715 ) and HC markers such as @entity17985 , TAT, @entity12075 and @entity12073 ; both HC markers ( @entity12073 , @entity12075 and @entity51611 ) and BEC markers ( @entity43956 and @entity29973 ) were detected in HOCs. Additionally, some HC markers were also significantly upregulated in hepatic stellate cells ( HSCs), sinusoidal endothelial cells (SECs) , Kupffer cells (KCs) and dendritic cells (DCs), mainly at 6-72 hours post partial hepatectomy (PH). CONCLUSION: Our findings indicate that there is a mutual transdifferentiation relationship between HC, BEC and HOC during LR, and a tendency for HSCs, SECs, KCs and DCs to transdifferentiate into HCs.
