Identification of genomic alterations in XXXX using array-based comparative genomic hybridization.

@entity1 @entity788 @entity10362 @entity71464 @entity5 @entity189 @entity16225 @entity30291 @entity24545 @entity32742 @entity71465 @entity23698 @entity3 @entity105 @entity9625
BACKGROUND: Genomic aberration is a common feature of @entity1 @entity5 and also is one of the basic mechanisms that lead to overexpression of oncogenes and underexpression of @entity5 suppressor genes. Our study aims to identify frequent genomic changes in @entity788 . MATERIALS AND METHODS: We used array comparative genomic hybridization (array CGH) to identify recurrent genomic alterations and validated the protein expression of selected genes by immunohistochemistry. RESULTS: Sixteen gains and thirty-two losses occurred in more than 30% and 60% of the @entity5 , respectively. High-level amplifications at 7q21.3-q22.1 and 19q13.2 and homozygous deletions at 1p33-p32.3, 1p22.1, 1q22, 3q27.2, 6p22.3, 6p21.31, 12q13.2, 17p13.2, 17q21.31 and 22q13.1 were identified. Especially, amplification of @entity23698 was detected in two @entity189 and homozygous deletion of @entity30291 in other two cases. In 15 independent validation samples, we found that @entity23698 (19q13.2) and @entity10362 (7q22.1) were amplified in 6 and 9 cases, and @entity71464 (17p13.2) and @entity32742 (17p13.2) were homozygously deleted in 3 and 1 cases. @entity23698 and @entity10362 were overexpressed, and @entity71464 and @entity32742 were underexpressed in @entity788 tissues than in morphologically normal operative margin tissues. Both GISTIC and Genomic Workbench software identified 22q13.1 containing @entity71465 and @entity24545 as the only homozygous deletion region. And the expression levels of @entity71465 and @entity24545 were significantly lower in @entity5 tissues than in morphologically normal operative margin tissues. Further validation showed that overexpression of @entity16225 was significantly associated with lymph node @entity3 , and overexpression of @entity9625 was significantly associated with invasive depth of @entity788 . CONCLUSION: These recurrent genomic changes may be useful for revealing the mechanism of pancreatic @entity105 and providing candidate biomarkers.
