Plasminogen activator activity in cumulus-oocyte complexes of gonadotropin-treated XXXX during the periovulatory period.

@entity1 @entity3208 @entity2975 @entity35 @entity11693 @entity214
Two types of plasminogen activator (tissue-type, tPA; urokinase-type, @entity11693 ) have been demonstrated in ovarian granulosa cells, but only tPA activity was found in denuded oocytes. Immature @entity35 were treated subcutaneously with 20 IU pregnant mare's serum gonadotropin (PMSG) to stimulate follicle maturation, followed 2 days later by an injection of 10 IU @entity1 chorionic gonadotropin (hCG) to induce ovulation. Cellular plasminogen activator activities were determined by @entity3208 ( @entity214 )- @entity2975 gel electrophoresis followed by a fibrin-overlay technique. Cumulus-oocyte complexes from @entity35 before and after PMSG treatment contained low amounts of tPA, but not @entity11693 , activity. After hCG treatment, tPA activity showed a time-dependent increase, reaching a maximum at 24 h after injection. At 12 and 24 h after hCG treatment, @entity11693 activity was also detected. The appearance of high molecular weight lysis zones further suggested the formation of plasminogen activator-inhibitor complexes. Morphological analysis indicated that the increases in oocyte tPA activity were correlated with the extent of cumulus cell expansion and dispersion. In denuded oocytes, tPA activity also progressively increased during the periovulatory period to a maximum at 24 h after hCG treatment. In contrast, neither @entity11693 activity nor activator-inhibitor complex was detected. Secretion of the proteases was measured in the conditioned media of cumulus-oocyte complexes cultured for 24 h in vitro. Substantial increases in tPA release were found in complexes obtained at 8 and 12 h after hCG injection, with lower secretion from complexes obtained at 24 h after hCG treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
