Diagnostic accuracy of serological assays in pediatric XXXX .

@entity1 @entity7571 @entity31 @entity29 @entity375 @entity30
BACKGROUND _ AIMS: Accurate serological assays are desirable for the diagnosis of @entity29 ( @entity29 ) types in the pediatric age group. The aim of this study was to test the diagnostic accuracy of modified assays for perinuclear (p) antineutrophil cytoplasmic antibodies (ANCAs) and anti- @entity375 cerevisiae antibodies (ASCAs) in @entity1 with pediatric @entity31 ( @entity31 ) and @entity30 ( @entity30 ) and in those without @entity29 . METHODS: With observers blinded to patients' diagnoses, serum specimens were analyzed for @entity7571 and IgG ASCAs and ANCAs by enzyme-linked immunosorbent assay. The perinuclear location of ANCAs visualized by indirect immunofluorescence was confirmed by its disappearance after administration of deoxyribonuclease. RESULTS: IgA and IgG ASCA titers were significantly greater and highly specific for @entity30 (95% for either, 100% if both positive). pANCA was 92% specific for @entity31 and absent in all non- @entity29 controls. The majority of @entity1 with @entity30 positive for pANCA had a @entity31 -like presentation. Disease location, duration, activity, complications, and treatment with immunosuppressive drugs did not have an impact on the ASCA or pANCA assay results. After resection, @entity31 @entity1 remained pANCA positive, in contrast to @entity1 with @entity30 , in whom ASCA titers decreased toward normal values postoperatively. CONCLUSIONS: ASCA and pANCA assays are highly disease specific for @entity30 and @entity31 , respectively. These serological tests can assist clinicians in diagnosing and categorizing @entity1 with @entity29 and may be useful in making therapeutic decisions.
