Genetic diversity of transmission-blocking vaccine candidates Pvs25 and Pvs28 in XXXX isolates from Yunnan Province, China.

@entity535 @entity70849 @entity3965 @entity70848 @entity311 @entity70847
BACKGROUND: Transmission-blocking vaccines (TBVs) have been considered an important strategy for disrupting the @entity311 transmission cycle, especially for @entity3965 @entity311 , which undergoes gametocytogenesis earlier during infection. Pvs25 and Pvs28 are transmission-blocking vaccine candidates for @entity3965 @entity311 . Assessment of genetic diversity of the vaccine candidates will provide necessary information for predicting the performance of vaccines, which will guide us during the development of @entity311 vaccines. RESULTS: We sequenced the coding regions of pvs25 and pvs28 from 30 @entity3965 isolates from Yunnan Province, identifying five @entity535 haplotypes of Pvs25 and seven @entity535 haplotypes of Pvs28. Among a total of four mutant residues, the predominant haplotype of Pvs25 only had the @entity70847 substitution. For Pvs28, a total of eight @entity535 substitutions were identified. The predominant haplotype of Pvs28 had two substitution at positions 52 ( @entity70848 ) and 140 ( @entity70849 ) with 5-6 GSGGE/D tandem repeats at the end of fourth EGF-like domain. Most @entity535 substitutions were common with previous reports from South Asian isolates. Although the nucleotide diversity of pvs28 (   = 0.0034    0.0012) was significantly higher than pvs25 (   = 0.0013    0.0009), it was still conserved when compared with the blood stage vaccine candidates. CONCLUSIONS: Genetic analysis revealed limited genetic diversity of pvs25 and pvs28, suggesting antigenic diversity may not be a particular problem for Sal I based TBVs in most @entity3965 -endemic areas of China.
