Identification of dominant pathogens in periapical lesions associated with persistent XXXX .

@entity1546 @entity3288 @entity5132 @entity797 @entity2523
OBJECTIVE: to identify dominant pathogens in the periapical lesions associated with persistent @entity1546 . METHODS: thirty-three root-filled teeth with persistent @entity1546 referred for surgical treatment were selected. Microbial samples were collected from the periapical lesions during apical surgery. Microbial identification was performed with species-specific primers prepared according to the sequence analysis data using a 16S rRNA technique. RESULTS: among the 33 cases, in 5 cases none of the target species were detected, 6 cases showed the presence of only one species, and 22 cases showed more than two species. @entity2523 endodontalis (45% of sample) was the most commonly detected dominant microbial species in the study sample, followed by Actinomyces viscosus (42%), @entity5132 (36%) and @entity2523 (27%). Fusobacterium, Actinomyces israelii and @entity797 were also detected in 27%, 21% and 15% of the sample, respectively. The most frequently isolated species, P. endodontalis, was in most cases detected together with Actinomyces (14 cases) and @entity2523 (6 cases). None of the lesions analysed in the present study contained @entity3288 . There was no correlation in relation to the presence of sinus tracts and the bacterial species. CONCLUSION: a mixed population of pathogens was found in the endodontic lesions associated with persistent @entity1546 . P. endodontalis, A. viscosus, @entity5132 and @entity2523 were the dominant species identified.
