Nuclear matrix protein alterations associated with XXXX metastasis to the liver.

@entity1 @entity694 @entity5 @entity3
PURPOSE: The development of @entity694 markers that can detect liver @entity3 early and predict which @entity1 are at risk to develop liver @entity3 would have a major impact on this disease. We have previously identified G. Brunagel, et al., @entity5 Research, 62:2437-2442, 2002, nuclear matrix proteins (NMPs), which are associated with @entity694 . The objective of this study is to identify the existence of a specific NMP "fingerprint" for @entity1 liver metastasis from @entity694 . EXPERIMENTAL DESIGN: Using high-resolution two-dimensional gel electrophoresis, we analyzed the NMP expression of 12 matched liver @entity3 and adjacent normal samples and three normal donor liver samples. These were compared with @entity694 NMP patterns, along with several primary cell systems and lines. RESULTS: Analysis of multiple gels for each sample revealed three proteins present in all liver @entity3 , which are not present in normal liver tissue and normal hepatocytes. These three proteins were also present in @entity694 samples. CONCLUSION: Data provided here demonstrate that the NMP composition is able to differentiate liver @entity3 from normal liver tissue and normal hepatocytes and that these proteins are also expressed in @entity694 . These results further show that the adjacent normal liver tissue changes its NMP pattern of expression. Development of an assay to detect these specific NMPs in tissue and/or serum specimens is a promising modality for early detection of liver @entity3 from @entity694 or potentially as a prognostic tool. In addition the functional characterization of these proteins will significantly enhance our understanding of the development of liver @entity3 of this disease.
