[Studies on the pathogenesis of @entity6 --immunochemical studies with monoclonal islet cell surface antibody using hybridization of spleen lymphocytes from non-obese XXXX @entity19 ].

@entity137 @entity6 @entity19 @entity9608 @entity3407 @entity95 @entity2975 @entity329 @entity35 @entity73202 @entity413
The production of monoclonal antibodies to islet cell surface antigens, using hybridization of spleen lymphocytes from non-obese @entity6 (NOD) @entity19 has been reported previously from our laboratory. In the present study, the immunochemical characteristics of the monoclonal antibody ( @entity73202 ) have been investigated using In-111 cells, a virus-induced @entity9608 cell line derived from the @entity329 as target cells. The antibody @entity73202 could be visually detected in the immunoenzymatic labelling of the surface of In-111 cells. To identify the molecular weight of target specific antigens reacting with @entity73202 , 125I-surface labelled In-111 cells were solubilized and extracts were absorbed with @entity73202 . The immunoprecipitates were subjected to @entity2975 gel electrophoresis and autoradiography. @entity73202 recognized two major polypeptides with apparent molecular weights of radioactive 64K and inactive 28K daltons. In order to evaluate antibody-mediated cytotoxic mechanisms of @entity73202 , complement-dependent antibody-mediated @entity137 (C'AMC) and @entity137 ( @entity137 ) were tested, using a method of specific @entity3407 release. In the study for C'AMC, even though over wide ranges of antibody concentration and @entity95 complement, purified @entity73202 had no apparent cytotoxic effects on In-111 cells. On the other hand, significant @entity137 was observed at 10 micrograms/ml antibody concentration and 1:40 target: effector cell ratio. Finally, the effect of @entity73202 on @entity413 -stimulated insulin release in isolated @entity35 islets was examined. At 16.7 mM @entity413 concentration, @entity73202 significantly inhibited the insulin release in the absence or presence of complement. Therefore, @entity73202 can not only bind but also be active to the target cells in the @entity137 and suppression of insulin release, and it can be a useful tool to clarify the pathogenesis of @entity6 . Furthermore, these results suggest that the relationship between islet cell surface antibody and cell-mediated immunity, especially immunoresponse against certain antigenic determinants on pancreatic B cells, seems to be important in the pathogenesis of @entity6 .
