Detection of XXXX DNA in the milk of naturally infected ewes.

@entity1 @entity742 @entity1042 @entity406 @entity281 @entity3252 @entity4801 @entity3204
@entity3252 is the major @entity4801 affecting @entity742 . It is important for veterinary medicine, animal science and public health since it causes reproductive and economic losses in the herd, as well as damaging @entity1 health due to consumption of contaminated meat and milk, which can facilitate zoonotic transmission. Detection of @entity3204 in ovine milk and lack of data in the literature describing differentiation between acute and chronic disease for this species stimulated the elaboration of the present research project. To achieve the aim of this study, the animals were allocated to two groups of 20 ewes each, of which group 1 was composed of animals with positive serology and group 2 with negative serology. Acute and chronic stages of the disease were differentiated by modified direct agglutination test (MAT), in which antigens were fixed with @entity406 (MAT-AF) and @entity1042 (MAT-AM). The parasite was detected in milk by polymerase chain reaction (PCR), and the molecular identity of the amplified products was confirmed by sequencing. The serological results indicated that @entity742 had a @entity281 profile. @entity3204 DNA was detected in seven milk samples from five seropositive @entity742 , and twice in milk of two @entity742 . Sequences of species shared 97-100% identity with @entity3204 . These findings allowed the hypothesis that the peripartum period may also lead to the resurgence of tissue @entity3204 tachyzoites cysts which can circulate again and be excreted in the milk. This study used @entity742 naturally infected with @entity3204 as a prerequisite for further investigations on the possible participation of this species in toxoplasmosis epidemiology and as a potential transmission route related to consumption of milk from infected @entity742 .
