Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a @entity35 model of Barrett's XXXX .

@entity355 @entity5 @entity2910 @entity17058 @entity71405 @entity418 @entity35 @entity420
UNASSIGNED: For many years, novel strategies for @entity5 detection and treatment using nanoparticles (NPs) have been developed. @entity420 is the sixth leading cause of @entity5 in Western countries, and despite recent advances in early detection and treatment, its prognosis is still very poor. This study investigated the use of fluorescent organic NPs as potential diagnostic tool in an experimental in vivo model of Barrett's @entity420 . NPs were made of modified polysaccharides loaded with @entity71405 ( @entity17058 ), a well-known fluorescent dye. The NP periphery might or might not be decorated with ASYNYDA peptide that has an affinity for @entity418 cells. Non-operated and operated @entity35 in which gastroesophageal reflux was surgically induced received both types of NPs (NP- @entity17058 and NP- @entity17058 -ASYNYDA) by intravenous route. Localization of mucosal NPs was assessed in vivo by confocal laser endomicroscopy, a technique which enables a "real time" and in situ visualization of the tissue at a cellular level. After injection of NP- @entity17058 and NP- @entity17058 -ASYNYDA, fluorescence was observed in @entity35 affected by @entity418 , whereas no signal was observed in control non-operated @entity35 , or in @entity35 with simple @entity2910 or @entity355 . Fluorescence was observable in vivo 30 minutes after the administration of NPs. Interestingly, NP- @entity17058 -ASYNYDA induced strong fluorescence intensity 24 hours after administration. These observations suggested that NPs could reach the @entity5 cells, likely by enhanced permeability and retention effect, and the peptide ASYNYDA gave them high specificity for @entity418 cells. Thus, the combination of NP platform and confocal laser endomicroscopy could play an important role for highlighting @entity418 conditions. This result supports the potential of this strategy as a targeted carrier for photoactive and bioactive molecules in @entity418 diagnosis and treatment.
