Differential expression of synaptic proteins after @entity102 in XXXX prefrontal cortex and hippocampus.

@entity72571 @entity59350 @entity21111 @entity44771 @entity102 @entity72570 @entity35 @entity22972 @entity72572
Prolonged stress has been associated with altered synaptic plasticity but little is known about the molecular components and mechanisms involved in the stress response. In this study, we examined the effect of @entity102 ( @entity102 ) on the expression of genes associated with synaptic vesicle exocytosis in @entity35 prefrontal cortex and hippocampus. @entity35 were stressed daily using a 21day restraint stress paradigm, with durations of half an hour or 6h. RNA and protein were extracted from the same tissue sample and used for real-time quantitative polymerase chain reaction (real-time qPCR) and immunoblotting, respectively. Focusing on the SNARE complex, we investigated the expression of the SNARE core components @entity22972 , @entity21111 , and @entity59350 at both transcriptional and protein levels. In addition, the expression of 10 SNARE regulatory proteins was investigated at the transcriptional level. Overall, the prefrontal cortex was more sensitive to @entity102 compared to the hippocampus. In prefrontal cortex, @entity102 induced increased mRNA levels of @entity59350 , @entity44771 , @entity22972 , @entity72570 , synaptotagmins I and III, and @entity72571 , whereas @entity21111 was down-regulated after @entity102 . Immunoblotting demonstrated equivalent changes in protein levels of @entity59350 , @entity22972 , and @entity21111 . In hippocampus, we found increased mRNA levels of @entity59350 and @entity72572 and a decrease in @entity44771 levels. Immunoblotting revealed decreased @entity59350 protein levels despite increased mRNA levels. Changes in the expression of synaptic proteins may accompany or contribute to the morphological, functional, and behavioral changes observed in experimental models of stress and may have relevance to the pathophysiology of stress-related disorders.
