XXXX carotid atherosclerotic plaque protein(s) change HDL protein(s) composition and impair HDL anti-oxidant activity.

@entity1 @entity217 @entity6635 @entity1675 @entity65
UNASSIGNED: High density lipoprotein (HDL) anti-atherogenic functions are closely associated with @entity65 risk factor, and are dictated by its composition, which is often affected by environmental factors. The present study investigates the effects of the @entity1 carotid plaque constituents on HDL composition and biological functions. To this end, @entity1 carotid plaques were homogenized and incubated with HDL. Results showed that after incubation, most of the @entity1675 ( @entity1675 ) protein was released from the HDL, and HDL diameter increased by an average of approximately 2 nm. In parallel, HDL antioxidant activity was impaired. In response to homogenate treatment HDL could not prevent the accelerated oxidation of LDL caused by the homogenate. Boiling of the homogenate prior to its incubation with HDL abolished its effects on HDL composition changes. Moreover, @entity6635 fluorescence quenching assay revealed an interaction between plaque component(s) and HDL, an interaction that was reduced by 50% upon using pre-boiled homogenate. These results led to hypothesize that plaque protein(s) interacted with HDL-associated @entity1675 and altered the HDL composition. Immuno-precipitation of @entity1675 that was released from the HDL after its incubation with the homogenate revealed a co-precipitation of three isomers of actin. However, @entity217 alone did not significantly affect the HDL composition, and yet the active protein within the plaque was elusive. In conclusion then, protein(s) in the homogenate interact with HDL protein(s), leading to release of @entity1675 from the HDL particle, a process that was associated with an increase in HDL diameter and with impaired HDL anti-oxidant activity.    2015 BioFactors, 42(1):115-128, 2016.
