Generation of Scaffoldless Hyaline Cartilaginous Tissue from XXXX iPSCs.

@entity1 @entity1707 @entity26885 @entity1841 @entity19 @entity5 @entity465 @entity2533 @entity35 @entity393 @entity44
UNASSIGNED: Defects in articular cartilage ultimately result in @entity393 . Repairing cartilage defects requires cell sources. We developed an  approach to generate scaffoldless hyaline cartilage from @entity1 induced pluripotent stem cells (hiPSCs). We initially generated an hiPSC line that specifically expressed GFP in cartilage when @entity44 was formed. We optimized the culture conditions and found @entity1841 , @entity2533 ( @entity2533 ), and @entity26885 critical for GFP expression and thus chondrogenic differentiation of the hiPSCs. The subsequent use of scaffoldless suspension culture contributed to purification, producing homogenous cartilaginous particles. Subcutaneous transplantation of the hiPSC-derived particles generated hyaline cartilage that expressed type II collagen, but not type I collagen, in @entity465 @entity19 . Transplantation of the particles into joint surface defects in @entity465 @entity35 and immunosuppressed mini- @entity1707 indicated that neocartilage survived and had potential for integration into native cartilage. The @entity465 @entity19 and @entity35 suffered from neither @entity5 nor ectopic tissue formation. The hiPSC-derived cartilaginous particles constitute a viable cell source for regenerating cartilage defects.
