Multicentre study of the molecular epidemiology, serotypes and antimicrobial susceptibility patterns of invasive XXXX invasive isolated from @entity1 in the Ille de France area.

@entity1 @entity2176 @entity115 @entity7646 @entity29154 @entity114 @entity2515 @entity2929 @entity2177 @entity3524 @entity3 @entity328
Between January 1997 and April 2002, 73 consecutive invasive strains of @entity328 were isolated from @entity1 under 16 years of age in four hospitals in suburban Paris. Their genetic diversity was investigated by serotyping and analysis of pulsed-field gel electrophoresis restriction patterns. Antibiotic susceptibility patterns were analysed by disk susceptibility testing and determination of minimal inhibitory concentrations. The genetic basis of @entity7646 resistance was investigated by polymerase chain reaction. Studies of @entity114 and @entity2176 tolerance were performed for each strain. Despite the high prevalence (45.2%) of @entity114 -nonsusceptible @entity328 , resistance to @entity115 (1.4%) was rare, and no strain was resistant to @entity3524 . Overall, 4.1% of pneumococcal strains were resistant to @entity114 . @entity114 or @entity2176 tolerance was not detected in any of the 73 strains studied. Of the @entity2929 -resistant strains (48%), all but one carried the ermB gene. No strains showing a decreased susceptibility to @entity2177 ( @entity29154 , >4 mg/l) or overexpressing an efflux pump inhibited by @entity2515 were isolated. The serotypes found, in order of frequency, were as follows: 18C, 14, 6B, 19F, 19A, 9V, 23F, 1, 7F, 9A, 38. Strains of @entity114 -nonsusceptible @entity328 belonged predominantly to serotypes 14, 6B, 9V, 9A, 23F, 19F and 19A. The seven-valent conjugated vaccine covered 85.5% of the serogroups isolated in @entity1 under 2 years of age and 65.6% of the serogroups identified in @entity1 over 2 years of age. The genetic analysis showed a high identity for some serotypes, such as 14/9V, 6B and 23F. The use of the seven-valent conjugated vaccine is a critical measure to prevent @entity3 in @entity1 in the Ille de France area.
