In Vitro Generation of IL-35-expressing XXXX Wharton's Jelly-derived Mesenchymal Stem Cells Using Lentiviral Vector.

@entity1 @entity32 @entity8990 @entity404 @entity19 @entity6978 @entity281 @entity403 @entity792
UNASSIGNED: @entity1 Wharton's Jelly-derived Mesenchymal Stem Cells (hWJ-MSCs) are easily available cells without transplant rejection problems or ethical concerns compared to bone-marrow-derived MSCs for prospective clinical applications. These cells display immunosuppressive properties and may be able to play an important role in @entity792 . Regulatory T-cells (Treg) are important to prevent @entity792 development. Interleukin 35 (IL-35) induces the proliferation of Treg cell populations and reduces the activity of T helper 17 (Th17) and T helper 1 (Th1) cells, which play a central role in @entity32 and @entity792 .Recent studies identified IL-35 as a new inhibitory cytokine required for the suppressive function of Treg cells. We created IL-35-producing hWJ-MSCs as a good vehicle for @entity32 and     @entity792 .     We     isolated     hWJ-MSCs     based     on     explant     culture.     HWJ-MSCs were transduced at MOI=50 ( @entity281 ) with lentiviral particles harboring @entity19 Interleukin 35 (mIL-35). Expression of IL-35 in hWJ-MSCs was quantified by an IL-35 ELISA kit.IL-35 bioactivity was analyzed by inhibiting the proliferation of @entity19 splenocytes using CFSE cell proliferation kit. Frequency of @entity404 +CD25+ @entity6978 low/neg @entity403 + Treg cells was measured by flow cytometry. There was an up to 85% GFP positive transduction rate, and the cells successfully released a high level of mIL-35 protein (750 ng/ml). IL-35 managed to inhibit @entity404 + T cell proliferation with @entity8990 , and improved the frequency of Treg cells.Our data suggest that transduced hWJ-MSCs overexpressing IL-35 may provide a useful approach for basic research on gene therapy for @entity792 .
