Evaluation of extracellular matrices and the plasminogen activator system in XXXX inner cell mass and trophectodermal outgrowth in vitro.

@entity73187 @entity73188 @entity742 @entity389 @entity65873
Effects of extracellular matrices (ECM) and the plasminogen activator (PA) system on outgrowth of @entity742 @entity389 ( @entity389 ) and trophectoderm in vitro were investigated. Experiment 1 evaluated the effects of plasminogen and ECM type on @entity389 and trophectodermal outgrowth, on glass Lab- @entity65873 chamber slides coated with collagen IV, fibronectin, or laminin. @entity389 outgrowth areas were reduced (p < 0.05) by plasminogen and were greatest (p < 0.05) on fibronectin. Trophectodermal outgrowth was not supported in this system. Experiment 2 evaluated the effects of @entity73187 ( @entity73187 ) or antiserum to urokinase-type PA (anti- @entity73188 ) on @entity389 outgrowth on fibronectin. Numbers of cells in the outgrowths were increased (p < 0.05) with @entity73187 , and anti- @entity73188 had no effect (p > 0.10). Experiment 3 evaluated the relationship between PA production and ECM type on @entity389 and trophectodermal outgrowth in microdrop cultures. PA production by @entity389 was greatest (p < 0.05) on fibronectin, but no differences (p > 0.10) were observed for trophectoderm. PA production was not correlated with @entity389 outgrowth areas (r = -0.12; p = 0.72) or numbers of cells in the @entity389 outgrowths (r = 0.09; p = 0.74) but was correlated with @entity389 areas (r = 0.75; p < 0.01) and numbers of cells in trophectodermal outgrowths (r = 0.57; p = 0.01). These results suggest that type of ECM, culture system, and alterations in the PA system influence cellular outgrowths by @entity389 and trophectoderm.
