A novel chemiluminescence paper microfluidic biosensor based on enzymatic reaction for XXXX determination.

@entity274 @entity60028 @entity414
In this work, chemiluminescence (CL) method was combined with microfluidic paper-based analytical device ( PAD) to establish a novel CL  PAD biosensor for the first time. This novel CL  PAD biosensor was based on enzyme reaction which produced @entity274 while decomposing the substrate and the CL reaction between @entity60028 derivative and generated @entity274 in acid medium. Microchannels in  PAD were fabricated by cutting method. And the possible CL assay principle of this CL  PAD biosensor was explained. @entity60028 derivative system was used to reach the purpose of high sensitivity and well-defined signal for this CL  PAD biosensor. And the optimum reaction conditions were investigated. The quantitative determination of @entity414 could be achieved by this CL  PAD biosensor with accurate and satisfactory result. And this biosensor could provide good reproducible results upon storage at 4  C for at least 10 weeks. The successful integration of  PAD and CL reaction made the final biosensor inexpensive, easy-to-use, low-volume, and portable for @entity414 determination, which also greatly reduces the cost and increases the efficiency required for an analysis. We believe this simple, practical CL  PAD biosensor will be of interest for use in areas such as disease diagnosis.
