Role of transcription factors in small intestinal ischemia-reperfusion injury and tolerance induced by XXXX preconditioning.

@entity63 @entity35 @entity4124 @entity4026 @entity5613 @entity6029
BACKGROUND: Small @entity63 -reperfusion (I/R) injury, a clinically important condition, induces severe organ damage. Ischemic preconditioning (IPC) produces tolerance to long-term I/R by inducing a short-term I/R. Herein, we have examined the reduction in the extent of injury by IPC. METHODS: Small intestinal I/R injury was induced in @entity35 by clamping the @entity6029 ( @entity6029 ) for 30 minutes followed by reperfusion for various 30 minutes. The IPC + I/R group underwent a short-term I/R (IPC) prior to long-term I/R. Nuclear factor-kB ( @entity4026 ) activity was analyzed by an electrophoretic mobility shift assay and cytokine mRNA levels, by reverse transcription-polymerase chain reaction. Apoptosis-related genes were analyzed by Western blotting and immunohistochemistry, and apoptotic cells, by TUNEL staining. RESULTS: The animals were subjected to 30 minutes of @entity63 followed by 30 minutes of reperfusion. @entity4026 activity increased in the I/R group and decreased in the IPC + I/R group. The IPC + I/R group showed decreased cytokine in mRNA levels. Expression of the proapoptotic gene @entity4124 was increased in the I/R and decreased in the IPC + I/R group. Expression of the antiapoptotic gene @entity5613 was increased in the IPC + I/R group. The number of apoptotic cells was increased in the I/R and decreased in the IPC + I/R group. CONCLUSION: Small intestinal I/R injury was reduced by IPC produced by clamping the @entity6029 ; thus, IPC may have potential clinical applications in the future.
