Ability of bivariate cytokeratin and deoxyribonucleic acid flow cytometry to determine the biologic XXXX of resectable @entity1149 .

@entity1 @entity146 @entity5 @entity1149 @entity2 @entity2802 @entity982
OBJECTIVE: The purpose of this study was to clarify the significance of bivariate cytokeratin and DNA flow cytometry for analysis of the biologic @entity146 of resectable @entity1149 . METHODS: In 92 @entity1 who underwent curative operations, the DNA ploidy status and S-phase fractions of the @entity5 cell populations inside the @entity5 were analyzed by a cytokeratin gating technique with @entity982 -embedded specimens and were correlated with the surgical results. RESULTS: Ninety @entity5 yielded assessable DNA histograms. DNA diploidy was detected in 25 @entity5 with a mean S-phase fraction of 14.3% +/- 4.7%, and DNA aneuploidy was detected in 65 @entity5 with a mean S-phase fraction of 15.1% +/- 7.1%. The 5-year overall and recurrence-free survivals were 73.3% and 70.3%, respectively. Multivariate analysis showed that only @entity2802 staging was a prognostic factor after surgery. There was a negative correlation between the logarithms of S-phase fraction and the disease-free interval for 22 @entity1 with proven recurrence (P =.006). The @entity5 with high S-phase fractions recurred more rapidly than did those with low S-phase fractions. CONCLUSION: In a bivariate analysis of cytokeratin and DNA flow cytometry in resectable @entity1149 , the S-phase fraction appeared to be correlated with the disease-free interval. However, DNA ploidy and S-phase fraction were not predictive of either recurrence or survival after operation. Thus DNA flow cytometry may be of limited use for the analysis of the biologic @entity2 .
