[The effect of transmembrane- and secreted- @entity5 XXXX factor-alpha on shock liver].

@entity326 @entity5 @entity39 @entity1505 @entity35 @entity30469 @entity526
OBJECTIVE: To observe the dynamic expression of transmembrane (TM)- @entity5 @entity39 factor ( @entity1505 and secreted (S)- @entity1505 in the development of @entity326 and explore the actions and mechanism of TM- @entity1505 in liver of the @entity35 with @entity326 . METHODS: @entity326 in @entity35 was induced by intravenous injection of dead gram negative bacteria @entity526 ; the kinetics of TM- @entity1505 on peritoneal macrophages and S- @entity1505 in serum of these @entity35 were determined. Pretreatment with @entity1505 converting enzyme antisense oligonucleotide (5 mg/kg) 30 minutes before @entity35 were administrated dead bacteria inhibited enzymatic cleavage of TM- @entity1505 into S- @entity1505 . Six hours after bacteria injection, TM- @entity1505 and S- @entity1505 were also detected respectively. The pathological injury in the livers of @entity35 with endotoxin shock was examined, and artery pressure was constantly measured. RESULTS: The kinetics of TM- @entity1505 expression in the development of @entity326 was different from that of S- @entity1505 expression in serum. The expression of TM- @entity1505 began to increase on the surface of peritoneal macrophages and liver within 30 min, after bacteria challenge and peaking within a period of 4.5 hours followed by a gradual decrease to a relatively high level which was maintained for at least 24 hours. The @entity30469 antisense oligonucleotide pretreated @entity35 showed remarkable increase in TM- @entity1505 expression by peritoneal macrophages and liver (P < 0.001), and their arterial blood pressure were maintained within normal levels and there were no detectable pathological changes in their livers. CONCLUSIONS: These findings suggested that TM- @entity1505 may be a potent endogenous regulator involved in anti-inflammatory responses to maintain normal arterial pressure and protect liver tissue from pathological injury in during endotoxin shock. This study confirmed the important role of @entity1505 in @entity326 which is not only of important theoretical significance, but also of practical interest in providing experimental basis for clinical treatment of endotoxin shock.
