Cardiac derived extracellular matrix enhances cardiogenic properties of XXXX cardiac progenitor cells.

@entity1 @entity5568 @entity19 @entity18964 @entity2279 @entity21505 @entity319 @entity29467 @entity6998 @entity15774
UNASSIGNED: The use of biomaterials has been demonstrated as a viable strategy to promote cell survival and cardiac repair. However, limitations on combinational cell-biomaterial therapies exist, as cellular behavior is influenced by the microenvironment and physical characteristics of the material. Among the different scaffolds employed for cardiac tissue engineering, a myocardial derived matrix has been shown to promote cardiogenesis in @entity19 cardiac progenitor cells (mCPCs) in vitro. In this study, we investigated the influence of the myocardial matrix hydrogel on @entity29467 -like @entity1 fetal and adult CPCs (fCPCs and aCPCs) when encapsulated in 3D in vitro. fCPCs encapsulated in the myocardial matrix showed an increase in the gene expression level of cardiac markers @entity15774 and @entity18964 and the vascular marker @entity319 after 4 days in culture, and a significant increase in @entity15774 up to 1 week. Increased gene expression levels of @entity21505 , @entity6998 , @entity319 and @entity2279 were also observed when aCPCs were cultured in the matrix compared to collagen. Cell survival was sustained in both hydrogels up to 1 week in culture with the myocardial matrix capable of enhancing the expression of the proliferation marker Ki-67 after 4 days in culture. When encapsulated CPCs were treated with @entity5568 , an improved survival of the cells cultured in the myocardial matrix was observed. Finally, we evaluated the use of the myocardial matrix as hydrogel for in vivo cell transplantation and demonstrated that the gelation properties of the hydrogel are not influenced by the cells. In summary, we showed that the myocardial matrix hydrogel promotes @entity1 CPCs cardiogenic potential, proliferation and survival and is a favorable hydrogel for 3D in vitro culture. Furthermore, we demonstrated the in vivo applicability of the matrix as a potential vehicle for cell transplantation.
