XXXX -induced @entity989 in the @entity19 .

@entity2479 @entity19 @entity9327 @entity26 @entity871 @entity2295 @entity2915 @entity941 @entity989
PURPOSE: To develop @entity26 -induced @entity989 in the @entity19 with reproducible and quantifiable @entity2479 suitable for examining pathogenesis and therapeutic intervention for @entity2479 in @entity989 ( @entity989 ) and other vasculopathologies. METHODS: One-week-old C57BL/6J @entity19 were exposed to 75% @entity26 for 5 days and then to room air. A novel fluorescein-dextran perfusion method has been developed to assess the vascular pattern. The proliferative neovascular response was quantified by counting the nuclei of new vessels extending from the retina into the vitreous in 6 microns sagittal cross-sections. @entity2915 were also stained for @entity9327 ( @entity9327 ). RESULTS: @entity941 -dextran angiography delineated the entire vascular pattern, including neovascular tufts in flat-mounted retinas. @entity871 -induced @entity2295 occurred at the junction between the vascularized and avascular retina in the mid-periphery. @entity2479 occurred in all the pups between postnatal day 17 and postnatal day 21. There was a mean of 89 neovascular nuclei per cross-section of 9 eyes in @entity871 compared to less than 1 nucleus per cross-section of 8 eyes in the normoxia control (P < 0.0001). Proliferative vessels were not associated with @entity9327 -positive astrocyte processes. CONCLUSIONS: The authors have described a reproducible and quantifiable @entity19 model of @entity26 -induced @entity2479 that should prove useful for the study of pathogenesis of @entity2479 as well as for the study of medical intervention for @entity989 and other @entity989 .
