Smooth muscle cell proliferation at the vascular anastomotic stricture in XXXX aortotomy model.

@entity35 @entity1975
BACKGROUND: We recently suggested that a @entity35 aortotomy model could be substituted for a vascular anastomotic stricture around a suture line. The aim of this study was to verify such a @entity35 aortotomy model using @entity1975 ( @entity1975 ) immunohistochemistry, which is a sensitive method for detection of proliferating cells in vascular tissue after injury. METHODS: Longitudinal aortotomy was performed in the abdominal aorta of the @entity35 subjects. The @entity35 were sacrificed at 1, 2, 4 and 8 weeks following aortotomy (n=20 in each group). The control @entity35 were sacrificed without aortotomy (n=20). The percentage of the lumen occluded by intimal thickening (I/M ratio) was calculated. All tissues were stained with antibodies against @entity1975 ( @entity1975 ). RESULTS: Values of I/M ratio were 8.5+/-4.4%, 15.6+/-9.5%, 11.8+/-5.5%, 9.6+/-6.2% at 1, 2, 4, 8 weeks following aortotomy in the injured groups and 0.4+/-0.1% in the controls, respectively. Those values in the injured group increased significantly as compared to the controls. There were also significant differences between one week and two weeks following aortotomy. The @entity1975 labeling index at one week following aortotomy (21.4+/-2.7%) was significantly higher than at two weeks following aortotomy (2.8+/-1.9%). SMCs in the intima at four and eight weeks following aortotomy were completely negative for @entity1975 . CONCLUSIONS: The experimental @entity35 aortotomy model was determined to be useful in the investigation of intimal thickening around the suture line.
