Analysis of Ki-67 antigen expression, DNA proliferative fraction, and survival in resected XXXX .

@entity1 @entity3123 @entity788 @entity894 @entity5 @entity1758
BACKGROUND: Prognostic markers for @entity788 , such as CEA, CA19-9, ploidy analysis, and S-phase determination using flow cytometry, have not been consistently predictive. We chose to evaluate nuclear proliferation, as measured by the @entity1758 monoclonal antibody and digital image analysis, as a prognostic marker in @entity3123 , and compare the findings with DNA ploidy and S-phase analysis. @entity1758 identifies the Ki67 antigen present in nuclei of cells in all phases of the cell cycle except G0. METHODS: We retrospectively reviewed 33 @entity1 with @entity788 resected for cure between 1989 and 1994 with available fixed tissue. Sectioned tissue was stained with @entity1758 , and the number of positively stained nuclei determined and expressed as a @entity1758 @entity894 ( @entity894 ) by quantitative image analysis. Disaggregated nuclei were analyzed by flow cytometry using standard techniques. RESULTS: @entity1758 @entity894 for @entity788 was heterogeneous within and between @entity5 . The @entity1758 @entity894 for the @entity5 was 28 +/- 15 (median 29). There was no correlation between survival and @entity1758 expression (R(2) = 0.03). Likewise, there was no correlation between @entity1758 @entity894 and percentage of cells in S-phase, G(2)/M, or total proliferating cells (S+G(2)/M; R(2) = 0.01), nor was there a difference between @entity1758 @entity894 and ploidy (P = 0.88). CONCLUSIONS: We conclude that in our @entity1 population, nuclear proliferation in @entity788 , as determined by expression of Ki67 nuclear antigen, does not appear to correlate with survival and is not a useful prognostic marker. Despite intuitive thoughts to the contrary, there is no correlation between cell cycle analysis as determined by flow cytometry and Ki67 expression in @entity788 . Current methods of assessing prognosis after curative resection of @entity788 , including lymph node and margin status, @entity5 size, and possibly DNA ploidy as determined by flow cytometry, are not augmented by the assessment of nuclear proliferation by image analysis using the @entity1758 monoclonal antibody.
