XXXX plays an essential role in bone development and postnatal bone formation.

@entity1603 @entity14901 @entity8334 @entity19 @entity15137 @entity6092
OBJECTIVES: To determine the role of @entity14901 in bone development and postnatal bone formation. METHODS: @entity15137 -Cre @entity19 were bred with @entity14901 (fx/fx) @entity19 to produce chondrocyte-specific @entity14901 conditional knockout (cKO) @entity19 . Embryonic skeletal preparation and staining were performed, alkaline phosphatase activity (ALP) and relative gene expression were examined in isolated primary cells. @entity14901 (fx/fx) @entity19 were also bred with @entity8334 -Cre @entity19 to produce osteoblast-specific @entity14901 cKO @entity19 . Postnatal bone formation was assessed by micro-computed tomography ( CT) and histological analyses in 2-month-old @entity19 . Mineralized bone nodule formation assay, @entity1603 ( @entity1603 ) labeling and gene expression analysis were performed. RESULTS: @entity19 with chondrocyte- and osteoblast-specific deletion of the @entity14901 gene are viable and fertile. Calvarial bone development was delayed in chondrocyte-specific @entity14901 cKO @entity19 . In osteoblast-specific @entity14901 cKO @entity19 , BMP signaling was partially inhibited and @entity19 developed an @entity6092 phenotype. Osteoblast proliferation and differentiation were impaired in osteoblast-specific @entity14901 cKO @entity19 . CONCLUSIONS: @entity14901 plays an essential role in bone development and postnatal bone formation.
