Comparison of the gene encoding, and the predicted XXXX composition of, platelet membrane receptor subunit glycoprotein Iba in members of the family Felidae.

@entity3534 @entity1 @entity548 @entity468 @entity715 @entity27463 @entity3689
BACKGROUND: There is minimal information regarding platelet receptors in the family Felidae. Comparative studies assist with identifying @entity3534 critical for protein structure and function. OBJECTIVE: The purpose of the study was to compare the gene encoding, and the predicted @entity3689 composition of, platelet membrane receptor subunit @entity27463 in Felidae family members. METHODS: Genomic DNA samples isolated from whole blood of 13 @entity468 and 50 big @entity468 representing 8 different species were subjected to PCR using primers designed to flank the coding region of @entity27463 in overlapping fashion. PCR products were separated via electrophoresis on agarose gels, and extracted products were submitted for sequencing. DNA sequences were used to predict the length and @entity3689 composition of the protein. RESULTS: Varying protein lengths were predicted in Felidae family members which were primarily due to polymorphisms in the variable number of tandem repeats region encoding the macroglycopeptide region of @entity27463 . Other areas of the gene and predicted @entity3689 compositions were fairly conserved when compared to @entity1 sequences and between Felidae family members. CONCLUSION: Various polymorphisms within @entity27463 , including length variants encoding the macroglycopeptide region, were identified in members of the family Felidae. More studies are needed to determine if a correlation exists between various polymorphisms and predisposition for @entity548 or @entity715 as suggested in @entity1 .
