The Biofilm Ring Test  : a rapid method for the routine analysis of XXXX biofilm formation kinetics.

@entity1 @entity852 @entity796 @entity1577
UNASSIGNED: Currently, few techniques are available for the evaluation of bacterial biofilm adhesion. These detection tools generally require time for culture and/or arduous handling steps.In this work, the Biofilm Ring Test   (BRT), a new technology, was used to estimate the biofilm formation kinetics of twenty-five strains of @entity796 , isolated from sputum of @entity1577 ( @entity1577 ) @entity1 . The new assay principle is based on the mobility measurement of magnetic microbeads mixed with a bacterial suspension in a @entity852 microplate. If free to move under the magnet action, particles gather to a visible central spot in the well bottom. Therefore, the absence of spot formation in the plate reflects the bead immobilisation by a biofilm in formation.The BRT device allowed us to classify the bacterial strains in three general adhesion profiles. The group 1 consists in bacteria, which are able to form a solid biofilm in less than two hours. The group 2 comprises the strains, which progressively set up a biofilm during 24h. Lastly, the group 3 includes the strains, which stay in a planktonic form. The belonging of our strains to these profiles didn't differ according to culture conditions as the use of different sets of beads or culture media.The BRT is shown to be an informative tool for the characterization of biofilm-forming bacteria. Various application perspectives could be investigated for that device, such as the addition of antibiotics to the bacterial suspension to select the ones, which would have the ability to inhibit the biofilm formation.
