XXXX 299: beneficial in vitro immunomodulation in cells extracted from inflamed human colon.

@entity32 @entity39 @entity5 @entity31 @entity38740 @entity1336 @entity526
BACKGROUND AND AIM: The present study determined the pattern of cytokine secretion (interleukin [IL]-1beta, @entity5 @entity39 factor [TNF]-alpha, interferon [IFN]-gamma and IL-10) and their cellular sources in mononuclear cells isolated from colonic mucosa from normal and @entity31 ( @entity31 ) in response to probiotic and pathogenic bacteria. METHODS: Mononuclear cells were extracted from normal and active @entity31 colonic mucosa and incubated with pure sonicates of probiotic, commensal, and pathogenic bacteria. Cytokine secretion was measured in culture supernatant and intracellular cytokine staining measured using fluorescent-activated cytometry. RESULTS: In mononuclear cells isolated from normal mucosa, significant increases in mean IL-1beta were observed with enteropathogenic @entity526 (286.3 +/- 138.7 pg/mL P < 0.05) and @entity526 (440.5 +/- 194.0 pg/mL P < 0.01) compared with unstimulated control cells (16.7 +/- 4.8 pg/mL). In contrast, mononuclear cells isolated from active @entity31 mucosa produced significant increases in mean IL-1beta in response to stimulation with @entity38740 (230.5 +/- 38.8 pg/mL P < 0.05), enteropathogenic @entity526 (231.7 +/- 45.3 pg/mL P < 0.05) and @entity526 (465.4 +/- 60.2 pg/mL P < 0.001) compared with unstimulated control cells (60.7 +/- 17.1 pg/mL). @entity526 also produced significant mean increases of TNF-alpha and IFN-gamma compared with unstimulated control cells. No significant increases in IL-1beta, TNF-alpha or IFN-gamma were observed with @entity1336 in cells derived from normal or inflamed mucosa. Strikingly, incubation of @entity1336 with mononuclear cells isolated from active @entity31 mucosa resulted in significant increases of mean IL-10 (327 +/- 53.5 pg/mL, P < 0.05) compared with unstimulated control cells (29.7 +/- 13.2 pg/mL). Intracellular cytokine staining confirmed T-cell and macrophage IL-10 production after @entity1336 stimulation. CONCLUSIONS: @entity1336 demonstrates beneficial immunomodulatory activity by increasing IL-10 synthesis and secretion in macrophages and T-cells derived from the inflamed colon. This may provide a mechanism through which probiotic bacteria ameliorate inappropriate @entity32 and induce tolerance.
