Suppression of XXXX oncogene expression by a polyamine-complexed triplex forming oligonucleotide in MCF-7 @entity0 cells.

@entity6136 @entity7225 @entity0 @entity517 @entity53 @entity4663 @entity1639 @entity7223
@entity4663 are excellent stabilizers of triplex DNA. Recent studies in our laboratory revealed a remarkable structural specificity of @entity4663 in the induction and stabilization of triplex DNA. @entity7225 ( @entity6136 ) showed optimum efficacy amongst a series of synthetic @entity7223 in stabilizing triplex DNA. To utilize the potential of this finding in developing an anti-gene strategy for @entity0 , we treated MCF-7 cells with a 37mer oligonucleotide to form triplex DNA in the up-stream regulatory region of the @entity517 oncogene in the presence of @entity6136 . As individual agents, the oligonucleotide and @entity6136 did not downregulate @entity517 mRNA in the presence of @entity53 . Complexation of the oligonucleotide with 2 mM @entity6136 reduced @entity517 mRNA signal by 65% at 10 microM oligonucleotide concentration. In contrast, a control oligonucleotide had no significant effect on @entity517 mRNA. The expression of @entity1639 oncogene was not significantly altered by the triplex forming oligonucleotide (TFO). @entity6136 was internalized within 1 h of treatment; however, it had no significant effect on the level of natural @entity4663 . These data indicate that selective utilization of synthetic @entity4663 and TFOs might be an important strategy to develop anti-gene-based therapeutic modalities for @entity0 .
