p- @entity8850 , @entity7764 , and NF-kB are involved in allergen-induced late-phase airway inflammation in asthma XXXX .

@entity1 @entity8850 @entity225 @entity4666 @entity56 @entity7764
BACKGROUND: Previous in vitro and animal studies demonstrated that transcription factors p- @entity8850 and @entity7764 are required to induce @entity4666 secretion by T helper (Th) 9 cells. It is believed that n factor-kappaB (NF-kB) plays a role in eosinophil survival. The importance of these transcription factors in the pathogenesis of @entity56 ( @entity56 ) in @entity1 is poorly understood. We evaluated p- @entity8850 and @entity7764 expression in peripheral blood Th9 cells and NF-kB expression in eosinophils during late-phase airway inflammation in @entity56 @entity1 . METHODS: Nineteen adults with @entity56 and 14 adult healthy individuals (HI) were examined. Peripheral blood collected 24  h before (baseline) and 24  h after bronchial allergen challenge. CD4(+) cells and eosinophils were isolated by high-density gradient centrifugation and magnetic separation. The percentage of Th9 cells and apoptotic eosinophils was estimated by flow cytometry. p- @entity8850 and @entity7764 expression was expressed as mean fluorescence intensity (MFI) in Th9 cells. NF-kB levels were expressed as MFI in peripheral blood eosinophils. Serum @entity4666 and @entity225 levels were determined by enzyme-linked immunosorbent assay. RESULTS: At baseline, MFI of p- @entity8850 and @entity7764 in peripheral blood Th9 cells and MFI of NF-kB in eosinophils and, serum @entity225 and @entity4666 levels were greater in @entity56 @entity1 (P   <   0.05). Decreased eosinophil apoptosis was seen in the @entity56 group compared with HI (P   <   0.05). MFI of p- @entity8850 , @entity7764 , and NF-kB and serum levels of @entity225 and @entity4666 were increased in the @entity56 group 24  h after challenge compared with baseline (P   <   0.05). In the @entity56 group, a correlation between serum @entity4666 and Th9 cells (r   =   0.7, P   =   0.001) and MFI of @entity7764 (r   =   0.6, P   =   0.01) 24  h after bronchial allergen challenge was observed. A correlation between Th9 cells and MFI of p- @entity8850 (r   =   0.45, P   =   0.03) as well as MFI of @entity7764 (r   =   0.5, P   =   0.02) 24  h after challenge was only observed in @entity56 @entity1 . A correlation between the MFI of NF-kB and eosinophil apoptosis was observed in @entity56 @entity1 24  h before (r   =   -0.46, P   =   0.02) and after (r   =   -0.5, P   =   0.02) challenge. DISCUSSIONS: p- @entity8850 and @entity7764 may be associated with Th9 cells and @entity4666 production, whereas NF-kB and @entity225 may be associated with reduced eosinophil apoptosis in allergen-induced late-phase airway inflammation. TRIAL REGISTRATION: ClinicalTrials.gov NCT02214303.
