222 antifungal agents as agricultural products, @entity45961 , @entity18389 , and @entity71764 , induced the expression of cytochrome p450 family and cell cycle-related genes in estrogen receptor expressing bg-1 XXXX cells.

@entity43 @entity4187 @entity71764 @entity18389 @entity2555 @entity4188 @entity2931 @entity5874 @entity4910 @entity3733 @entity2556 @entity98 @entity13323 @entity1299 @entity45961
@entity45961 , @entity18389 , and @entity71764 are antifungal agents used in agricultural applications, which are present at measurable amounts in fruits and vegetables. The induction of @entity13323 gene expression including @entity4187 and @entity4188 is mediated by the transformation of @entity2555 ( @entity2556 ), and modulated by @entity5874 ( @entity5874 ). @entity4187 is expressed in the liver, pancreas, thymus, uterus, and small intestine. @entity4188 is abundant in the prostate, breast, and uterus. Expression levels of @entity4187 and @entity4188 indicate @entity2556 -induced immunotoxicity, oxidative stress, and activation of environmental carcinogens. In this study, the ability of cell viability was examined as @entity98 assay by pesticides; @entity45961 , @entity18389 and @entity71764 . In addition, expression levels of mRNA and protein of @entity5874 , @entity4187 , and @entity4910 were analysed by RT-PCR and Western blot analysis in BG-1 @entity43 cells with @entity2931 receptors (ER). To evaluate the ability of cell viability, BG-1 cells were cultured with a negative control (0.1% @entity3733 ), 17b-oestradiol (E2; 1*10(-9)M), @entity45961 , @entity18389 or @entity71764 (10(-5)-10(-8)M). To evaluate the expression levels of mRNA and protein, BG-1 cells were cultured with a negative control (0.1% @entity3733 ), 17b-oestradiol (E2; 10(-9)M) and these pesticides (10(-5)M). As results, E2 as a positive control markedly increased BG-1 cell viability ~5 times compared to a negative control (P<0.05). In addition, treatments with these pesticides increased BG-1 cell viability at the concentrations of 10(-8) and 10(-5)M about from 1.5 to 2 times, respectively (P<0.05). When respective treatment co-treated with @entity1299 , an ER antagonist, BG-1 cell viability was reversed to the level of a negative control. The mRNA expression of @entity4187 was increased by E2, @entity45961 , and @entity71764 in a time-dependent manner but not by @entity18389 , while its level was reversed in the presence of @entity1299 . In parallel with their transcriptional levels, protein levels of @entity4187 and @entity4910 were induced by E2 and these pesticides, while the level of @entity5874 was not altered by E2 and these pesticides. Taken together, these results imply that the pesticides, @entity45961 , @entity18389 , and @entity71764 , may have disruptive effects on ER expressing cells or tissues by alteration of @entity4187 and @entity4910 via an ER-dependent pathway.
