[Proliferative activity and XXXX expression in transitional cell carcinoma of the urinary bladder].

@entity1 @entity1364 @entity2735 @entity5 @entity876 @entity1606
Mutations of the @entity876 gene are important mechanisms in malignant transformation and are associated with dysregulation of normal cell growth. In the present study the expression of mutated @entity876 -protein and @entity1364 ( @entity1364 ) was investigated in a series of 31 @entity1 @entity1606 ( @entity1606 ) by immunohistochemistry (IHC). The number of @entity1364 -positive cells and the pattern of expression was distinct in normal urothelium being confined to the basal cell layer. In dysplastic urothelium and in @entity2735 ( @entity2735 ) @entity1364 -immunoreactive nuclei were irregularly distributed throughout all layers. In @entity5 cell complexes the pattern of @entity1364 -immunoreactivity was different in papillary and primary infiltrating TCCs. Densitometric quantification of the intensity of the @entity1364 -reactivity using image analysis revealed an increase from normal to dysplastic urothelium and from dysplastic urothelium to invasive @entity5 . 21/31 (68%) of the @entity5 and @entity5 -associated @entity2735 showed overexpression of @entity876 varying in percentage, pattern and reaction intensity. The percentage of @entity1364 -positive cells was higher in @entity5 overexpressing @entity876 . Double IHC showed colocalization of both molecules in a significant proportion of @entity5 cells suggesting a link of @entity876 overexpression and the abnormal proliferative activity. The present results show that @entity876 over-expression is found in a significant percentage of TCCs and indicate a close association with a defective growth regulation resulting in increased @entity1364 -levels and enhanced cellular proliferation.
