XXXX regulates cytokine-mediated communication between cooperating cell types during collective border cell migration.

@entity29001 @entity5 @entity40182 @entity29000 @entity72016 @entity238
UNASSIGNED: Collective cell migration is emerging as a major contributor to normal development and disease. Collective movement of border cells in the @entity238 ovary requires cooperation between two distinct cell types: 4-6 migratory cells surrounding two immotile cells called polar cells. Polar cells secrete a cytokine, Unpaired ( @entity40182 ), which activates @entity29000 / @entity29001 signaling in neighboring cells, stimulating their motility. Without @entity40182 , migration fails, causing sterility. Ectopic @entity40182 expression is sufficient to stimulate motility in otherwise immobile cells. Thus regulation of @entity40182 is key. Here we report a limited RNAi screen for nuclear proteins required for border cell migration, which revealed that the gene encoding @entity72016 ( @entity72016 ) is required in polar cells for @entity40182 expression without affecting polar cell fate. In the absence of @entity72016 , fewer border cells are recruited and motility is impaired, similar to inhibition of @entity29000 / @entity29001 signaling. We further show @entity72016 in polar cells is required for @entity29000 / @entity29001 activation in border cells. Genetic interactions further confirmed @entity72016 as a new regulator of @entity40182 / @entity29000 / @entity29001 signaling. These findings shed light on the molecular mechanisms regulating the cooperation of motile and non-motile cells during collective invasion, a phenomenon that may also drive @entity5 .
