Inhibition of steroidogenic @entity1447 enzymes in @entity35 testis by XXXX and related @entity8304 anti-fungal drugs.

@entity1 @entity1447 @entity251 @entity1707 @entity19 @entity3102 @entity8304 @entity701 @entity342 @entity14257 @entity35 @entity23411 @entity165
@entity3102 , an @entity8304 antifungal drug, has previously been shown to diminish @entity251 and @entity701 production in @entity1 as well as @entity35 and @entity19 cells in vitro. Inhibition of adrenal mitochondrial @entity1447 enzymes was demonstrated. In this study we tested several @entity8304 antifungal drugs and examined the individual steps in testicular steroidogenesis to determine which enzymes in the @entity342 pathway were blocked. In addition, we studied @entity14257 24-hydroxylase activity in cultured @entity1707 kidney cells (LLC-PK1) to assess a mitochondrial P-450 enzyme in another organ. All @entity23411 tested inhibited both total @entity251 production and 24-hydroxylase activity but the relative potencies differed. We next studied the individual testicular enzymatic steps between @entity165 and @entity251 . @entity3102 inhibited @entity165 -side-chain-cleavage enzyme (mitochondrial) and C-17,20 lyase (microsomal). The three inhibited enzymes (two testicular and one renal) are all P-450 cytochromes. Testicular 17-hydroxylase, also a P-450 cytochrome, was not inhibited even at high doses of @entity3102 . This is an interesting finding because the testicular hydroxylase and lyase have been shown to be a single protein. Non- @entity1447 enzymes in the @entity342 pathway were not inhibited. The results demonstrate that several @entity8304 antifungal drugs all inhibit both microsomal and mitochondrial @entity1447 enzymes in multiple organs.
