Effects of a selective DP receptor agonist (BW 245C) and antagonist (BW @entity71663 ) on the XXXX colonic epithelium: an argument for a different DP receptor?

@entity1672 @entity55395 @entity235 @entity1421 @entity71663 @entity71662 @entity95 @entity2918 @entity857
Earlier studies showed that @entity1672 produced both increases and decreases in short-circuit current across the @entity235 proximal colon. @entity1672 metabolites had opposing effects: @entity71662 elicited only increases in Isc, and @entity55395 elicited only decreases. The stimulant effects involved FP receptors, but the receptors involved in mediating the inhibitory effects remained undefined. Here we show that the tissue, although it is capable of producing both @entity1672 and @entity1421 , did not produce @entity71662 in measurable amounts. The selective DP receptor agonist BW 245C did not mimic the inhibitory effects of @entity1672 , producing only dose-dependent increases in short-circuit current. Further, these responses were not significantly inhibited by BW @entity71663 . Cross-desensitization experiments suggested that the stimulant effects of BW 245C involved the EP receptor. However, on a standard preparation ( @entity95 platelets), both @entity1672 and BW 245C inhibited @entity857 -induced aggregation and were antagonized by BW @entity71663 . @entity71662 had no effects. The decreases in short-circuit current across the @entity235 colonic epithelium elicited by @entity1672 and @entity55395 are not mimicked by other @entity2918 , nor by the selective agonist BW 245C, and thus appear to involve receptors other than the classical DP receptor.
