XXXX inhibits @entity1 @entity850 -derived fibroblast proliferation, metabolism, and function in vitro.

@entity1 @entity26 @entity3733 @entity3446 @entity850 @entity855
OBJECTIVE: To determine if @entity3446 inhibits the growth, function, and metabolism of @entity1 @entity850 ( @entity850 )-derived fibroblasts. STUDY DESIGN: Controlled in vitro study. SETTING: Tertiary care hospital in a research university. SUBJECTS AND METHODS: Fibroblasts isolated from biopsies of 5 @entity1 with @entity850 were cultured. Cell proliferation, histology, gene expression, and cellular metabolism of @entity850 -derived fibroblasts were assessed in 4 conditions: (1) fibroblast growth medium, (2) fibroblast growth medium with @entity3733 ( @entity3733 ), (3) fibroblast growth medium with 10(-10) M (low-dose) @entity3446 dissolved in @entity3733 , and (4) fibroblast growth medium with 10(-9) M (high-dose) @entity3446 dissolved in @entity3733 . RESULTS: The @entity850 fibroblast count and DNA concentration were reduced after treatment with high-dose @entity3446 compared to @entity3733 (P = .0007) and normal (P = .0007) controls. Collagen I expression decreased after treatment with high-dose @entity3446 versus control (P = .0051) and @entity3733 (P = .0093) controls. Maximal respiration decreased to 68.6 pMoles of @entity26 /min/10 mg/protein from 96.9 for @entity3733 (P = .0002) and 97.0 for normal (P = .0022) controls. @entity855 ( @entity855 ) production decreased to 66.8 pMoles from 88.1 for @entity3733 (P = .0006) and 83.3 for normal (P = .0003) controls. Basal respiration decreased to 78.6 pMoles from 108 for @entity3733 (P = .0002) and 101 for normal (P = .0014) controls. CONCLUSIONS: @entity3446 demonstrated an anti-fibroblast effect by significantly reducing the proliferation, metabolism, and collagen deposition of @entity1 @entity850 fibroblast in vitro. @entity3446 significantly decreased oxidative phosphorylation of @entity850 fibroblasts, suggesting at a potential mechanism for the reduced proliferation and differentiation. Furthermore, @entity3446 's anti-fibroblast effects indicate a promising adjuvant therapy for the treatment of @entity850 .
