Tissue factor-dependent and -independent pathways of @entity262 activation in XXXX : a single-center cohort study.

@entity1 @entity7 @entity39 @entity262 @entity3052 @entity5642 @entity8624 @entity1583 @entity880
BACKGROUND: In @entity3052 ( @entity3052 ), @entity5642 ( @entity5642 ) contributes to morbidity and mortality, but the underlying pathomechanisms remain incompletely understood. METHODS: We conducted a prospective study on 69 @entity1 with newly diagnosed @entity3052 to further define the correlates of @entity262 activation in this @entity1583 . Tissue factor procoagulant activity (TF PCA) of isolated peripheral blood mononuclear cells (PBMCs) and TF expression by circulating microparticles (MPs) were assessed by single-stage clotting and thrombin generation assay, respectively. Soluble plasma TF antigen and secretion of @entity7 ( @entity7 ) by cultured PBMCs were measured by ELISA. Cell-free plasma DNA was quantified by staining with a fluorescent dye. RESULT: TF PCA of PBMCs was significantly increased in @entity3052 @entity1 as compared to healthy controls. Furthermore, TF PCA was significantly associated with decompensated @entity5642 at presentation, as defined by a plasma fibrinogen level of  <=1  g/L (n  =  11). In addition to TF PCA and circulating blasts, serum @entity880 dehydrogenase, a surrogate marker for @entity8624 , correlated with plasma D-Dimer in the total @entity1 cohort and was significantly increased in @entity5642 @entity1 , suggesting a role for myeloblast apoptosis/ @entity39 in activation of the TF-dependent coagulation pathway. Consistently, TF-bearing plasma MPs were more frequently detected and levels of soluble TF antigen were significantly higher in @entity5642 vs. non- @entity5642 @entity1 . No association was found between TF PCA expression and @entity7 secretion by isolated PBMCs, but significantly increased levels of cell-free plasma DNA pointed to a contribution of the intrinsic contact pathway to @entity262 activation in the total @entity1 cohort and in @entity1 with lower TF PCA expression. While PBMC-associated TF PCA had no effect on long-term survival, @entity5642 occurrence at presentation increased the risk of early mortality. CONCLUSION: In newly diagnosed @entity3052 , TF expression by PBMCs and shedding of TF-bearing plasma MPs are central to the pathogenesis of @entity5642 , but additional pathways, such as DNA liberation, may contribute to @entity262 activation.
