Genomic comparison of @entity4021 virus R strain and its XXXX -passaged @entity536 .

@entity1522 @entity4021 @entity2036 @entity1573 @entity536
The present study examined the genomic differences between @entity4021 virus (FMDV) R strain and its attenuated, @entity1573 -passaged (R(304)) strain. Eleven pairs of primers were used to amplify the complete genome of FMDV R and R(304) by RT-PCR. Each fragment was cloned into pMD18-T vector and sequenced. Nucleotide analyses showed that the genome encoding regions of R and R(304) strains open reading frame (ORF) were both 6966 @entity2036 (nt) in length, encoding 2322 @entity1522 . One hundred and ten @entity2036 or 32 @entity1522 were found to be mutated most frequently were in the 3A gene. The next highest rates of mutation were observed in the LP and 1D genes. No mutations were found in either the 2A or 2C genes. The length of 5'IRES region and 3'UTR were 450 nt and 94 nt, respectively. The 5'IRES region and 3'UTR had only 4 nt and 3 nt mutation, respectively after attenuation. The R(304) poly(A) tail length of 18 nt, while that of the R strain was 30 nt. This result demonstrated the primary genomic changes of a FMDV and its @entity536 , which has important implications in understanding the molecular epidemiology and functional genomics of FMDV.
