Phospholipid concentration in lung lavage fluid as biomarker for XXXX .

@entity1 @entity32 @entity3794 @entity358 @entity3440 @entity35
Pulmonary surfactant comprised primarily of phospholipids is a phospholipid-protein complex synthesized by type II alveolar epithelial cells or Clara cells and secreted to the pulmonary alveoli. As changes have been found in phospholipid concentrations in the bronchoalveolar lavage fluid (BALF) of @entity1 with @entity3794 , phospholipid is considered to be involved in the process of fibrois/fibrotic process. Therefore, we made a crystalline @entity3440 @entity35 model and measured phospholipid concentrations in lung lavage fluid in order to study the relationship of phospholipid to particle-induced @entity3794 . Eight-week-old @entity35 male @entity35 (n = 35) were injected with 2 mg crystalline @entity3440 particles suspended in 0.4 ml physiological saline. @entity35 in the control group (n = 35) were injected with physiological saline only. There were 7 @entity35 in each of the ten subgroups. @entity35 were sacrificed and dissected at 3 days, 1 wk, 1 mo, 3 mo, and 6 mo after injection. Bronchoalveolar lavage was conducted on bronchoalveoli recovered from the right lung of each @entity35 , the lavage fluid was centrifuged, and the supernatant was used to measure phospholipid concentration. The results were compared with previously reported @entity32 scores. Phospholipid concentrations in lung lavage fluid for the exposed group showed a statistically significant increase compared to the control group throughout the observation period. Moreover, when compared to histopathologically examined @entity32 scores, a positive correlation was found between the two. Judging from the facts that phospholipid concentrations in lung lavage fluid increased and that this increase correlated with the severity of @entity32 , this experiment indicated that phospholipids are involved in particle-induced @entity358 .
