@entity263 xenografts with large-cell and small-cell features derived from a single @entity1 's XXXX : morphological, immunohistochemical, and gene expression profiles.

@entity1 @entity263 @entity4153 @entity5 @entity19 @entity915 @entity3270 @entity152 @entity420 @entity1865
BACKGROUND: @entity4153 ( @entity4153 ) of the prostate is an @entity263 found at progression in 10-20% of castrate-resistant disease. Its finding predicts a distinct clinical course and a poor prognosis. @entity152 ( @entity152 ) is a much rarer variant that behaves similarly to @entity4153 . The biological mechanisms that drive these disease variants are poorly understood. METHODS: Eight @entity5 fragments from the salvage pelvic exenteration specimen of a @entity1 with castrate-resistant @entity263 were subcutaneously implanted into 6- to 8-week-old male CB17 @entity915 @entity19 . Serial tissue sections and tissue microarrays of the resulting @entity1865 PCa 144 xenograft lines were used for histopathologic and immunohistochemical characterization of the xenografts and their tissue of origin. RNA from two representative xenograft sublines was used for gene-expression profiling. RESULTS: All eight fragments formed @entity5 : four of the @entity1865 PCa 144 xenograft sublines had morphologic characteristics of @entity4153 and four, of @entity152 . All retained high fidelity to their parent @entity5 tissue, which remained stable through serial passages. Morphological transitions in the specimen of origin suggested @entity152 represents an intermediate step between @entity420 and @entity4153 . Over 2,500 genes were differentially expressed between the @entity4153 ( @entity1865 PCa 144-13) and the @entity152 ( @entity1865 PCa 144-4) sublines and enriched in "Nervous System Development" Gene Ontology subtree. CONCLUSION: The eight xenograft models described represent the spectrum of @entity3270 in @entity263 and will be valuable preclinical tools to study the pathogenesis of and therapy targets for this increasingly recognized subset of @entity263 .
