Induced Pluripotent Stem Cell-Derived Natural Killer Cells for Treatment of XXXX .

@entity43 @entity1 @entity19 @entity5 @entity5168 @entity6440
UNASSIGNED: Natural killer (NK) cells can provide effective immunotherapy for @entity43 . Here, we evaluated the ability of NK cells isolated from peripheral blood (PB-) and NK cells derived from induced pluripotent stem cell (iPSC-) to mediate killing of @entity43 cells in a @entity19 xenograft model. A @entity19 xenograft model was used to evaluate the intraperitoneal delivery of three different NK cell populations: iPSC-derived NK cells, PB-NK cells that had been activated and expanded in long-term culture, and overnight activated PB-NK cells that were isolated through @entity5168 / @entity6440 depletion of peripheral blood B and T cells. Bioluminescent imaging was used to monitor @entity5 burden of luciferase expressing @entity5 lines. Tumors were allowed to establish prior to administering NK cells via intraperitoneal injection. These studies demonstrate a single dose of any of the three NK cell populations significantly reduced @entity5 burden. When @entity19 were given 3 doses of either iPSC-NK cells or expanded PB-NK cells, the median survival improved from 73 days in @entity19 untreated to 98 and 97 days for treated @entity19 , respectively. From these studies, we conclude iPSC-derived NK cells mediate anti- @entity43 killing at least as well as PB-NK cells, making these cells a viable resource for immunotherapy for @entity43 . Due to their ability to be easily differentiated into NK cells and their long-term expansion potential, iPSCs can be used to produce large numbers of well-defined NK cells that can be banked and used to treat a large number of @entity1 including treatment with multiple doses if necessary. This article is protected by copyright. All rights reserved.
