[Detection of telomerase XXXX gene expression of exfoliated cell in broncho-alveolar lavage fluid].

@entity1 @entity6437 @entity2 @entity2215
BACKGROUND _ OBJECTIVES: The @entity1 with @entity2 usually have high telomerase activity, the pseudopositive result is happen frequently when the test is done with traditional method. Telomerase activity is more strongly correlated with @entity2215 mRNA. This study was designed to detect the expression of telomerase @entity2215 gene in broncho-alveolar lavage fluid of the @entity1 with @entity2 by relative quantitative RT-PCR method, to afford a useful tool for early and differentiate diagnosis of @entity2 . METHOD: The exfoliated cells in broncho-alveolar lavage fluid from 20 @entity1 @entity2 and 10 health @entity1 were analyzed for @entity2215 expression by relative quantitative RT-PCR method, in the same time a part of exfoliated cells were detected with pathologic method. After operation, pathologic detection was performed for all samples. RESULTS: Of 20 @entity1 @entity2 and, 19 @entity1 were @entity2 , 1 @entity1 was @entity6437 in pathological diagnosis after operation. The relative expression of telomerase @entity2215 gene in 16 cases of 19 @entity1 with @entity2 were detected with the relative quantitative RT-PCR, the positive rate is 84.2% (16/19), the expressions of telomerase @entity2215 gene were at various quantities, average levels is 0.42. The positive rate in exfoliated cells detected with pathologic method were 57.9% (11/19). There was a statistical difference between them(P < 0.01). Ten normal @entity1 were @entity2215 gene expression negative. CONCLUSIONS: Relative quantitative RT-PCR were more sensitive than cell morphologic method in detecting telomerase @entity2215 gene expression in broncho-alveolar lavage fluid of @entity1 with @entity2 . Detecting quantitatively the levels of telomerase @entity2215 gene expression in broncho-alveolar lavage fluid will may be useful for early and differentiate @entity2 , especially for @entity2 .
