Big prolactin 60 kDa is overexpressed in salivary glandular epithelial cells from XXXX with Sj  gren's syndrome.

@entity1 @entity11746 @entity146 @entity12595 @entity4090 @entity10632
Characterization of endogenous synthesis of prolactin ( @entity4090 ) proteins and their cellular localization in labial salivary glands of @entity1 with @entity10632 ( @entity10632 ) were achieved. @entity4090 , @entity12595 ( @entity12595 ), and @entity11746 protein were detected by immunohistochemistry. In situ prolactin synthesis was investigated in controls and @entity10632 @entity1 by ex vivo incubation of minor salivary glands biopsies and immunoprecipitation assay. Increased @entity4090 -immunoreactivity was found in cytoplasmic acinar epithelial cells in @entity10632 @entity1 compared with normal subjects. @entity12595 was distributed only in ductal epithelial cells in which @entity11746 protein (a @entity12595 -associated protein) was also present. @entity4090 , @entity12595 , or @entity11746 -immunoreactivity was not detected in infiltrating mononuclear cells. Immunoprecipitation demonstrated that @entity4090 synthesis occurred in minor salivary glands with increased synthesis of two distinct @entity4090 -like proteins (one major band at 60 kDa and a minor at 16 kDa) in @entity10632 glands compared with normal glands. Expression of @entity4090 gene was demonstrated in @entity10632 salivary glands using RT-PCR. A positive correlation was found between the presence of @entity4090 -like proteins in acinar epithelial cells of @entity10632 @entity1 and clinical extraglandular manifestations. The presence of anti-Ro and anti-La antibodies also positively correlated with a higher percentage of @entity4090 in acinar epithelial cells. In conclusion, @entity4090 -like proteins are synthetized and overexpressed in glandular epithelial cells of labial salivary glands from @entity10632 @entity1 and correlate with the @entity146 .
