Sucrosemia in XXXX : a potential screening test.

@entity7016 @entity969 @entity1593
During studies to develop serum tests of small intestinal permeability, we detected an unidentified disaccharide in HPLC traces of sera from untreated celiacs. This present study aimed to identify the disaccharide and determine whether the presence of the disaccharide in the serum after an oral challenge had potential as a simple screening test for celiac disease. The @entity7016 was identified as @entity969 by incubation studies of sera with disaccharidases. Twenty untreated celiacs, 15 treated celiacs, and 20 normal or dyspeptic controls were studied for the presence of @entity969 in their serum after an oral load (8 g). The results in celiacs were compared with the presence of serum IgA endomysial antibodies. The 10 normal controls were also given a larger @entity969 challenge (50 g). Ten of the untreated celiacs and 10 controls had their brush border disaccharidase activities measured. @entity969 eluted in the same position as the unidentified disaccharide in the HPLC trace and the latter could be removed by incubation with sucrase. All untreated celiacs but none of the treated celiacs had @entity969 in their serum after the 8-g oral challenge. None of the controls had @entity969 in their serum after the 8-g or 50-g challenges. Three untreated celiacs were IgA endomysial antibody negative as were all the treated cases. Brush border sucrase activity was low in @entity1593 . The presence of @entity969 in the serum after an oral load shows promise as a noninvasive test for celiac disease.
