Detection of differentially expressed genes in XXXX using array-filter analysis.

@entity1 @entity691 @entity1357 @entity66 @entity13062 @entity3875 @entity310 @entity1356 @entity1193 @entity3061 @entity2841
The precise cellular mechanism of @entity3061 in @entity66 is still unknown. The aim of the study was to screen for new gene products in macrophages during particle contact. METHOD: In an established macrophage model @entity1357 -cells ( @entity1 monocytic cells) were differentiated under the influence of @entity3875 and @entity2841 into macrophage-like cells (MLC). MLCs were incubated each with different concentrations of @entity691 particles, Lipopolysaccharids (LPS) and controls. Isolated RNA was transcribed into complementary radioactive 32P labeled cDNA. This probe was hybridised on an @entity1 cDNA expression array and analysed by autoradiography. To obtain a more reliable method quantifying mRNA, the reverse transcriptase polymerase chain reaction (RT-PCR) was used. RESULTS: The arrays showed an upregulation of the following genes by particles: TNF-Rezeptor 2, @entity1193 , Bone Morphogenic Protein 4 and HM 145. This was proven three times using RT-PCR and statistically significant in comparison to the controls. LPS induced the same upregulation except for HM145 whereas particles caused downregulation of this mRNA expression. CONCLUSION: Our results prove that the model of differentiated THP-1 cells treated with PE particles is a suitable system to analyse differential gene expression patterns, since the induction of the major positive control genes @entity310 and @entity1356 were detected by this approach. @entity13062 is known as signal protein which mediates ectopic bone formation and can also be interpreted as a contra regulatory gene. HM 145 belongs to the leukocyte chemotactic peptide receptor family. HM 145 seems to be one of the first genes that is enhanced along the septical pathway but less expressed by contact with particles. Analysis of HM 145 expression might help to diagnose septic versus aseptic loosening of prosthesis.
