[Correlation of expression of XXXX and matrix matalloproteinase-2 to invasion of @entity43 cells in vitro].

@entity43 @entity1 @entity1476 @entity5 @entity7 @entity3
BACKGROUND _ OBJECTIVE: @entity7 ( @entity7 ) and @entity1476 ( @entity1476 ) are the important factors in @entity5 @entity3 . To the author's knowledge, their relationship has not been addressed to date. This study was designed to investigate the in vitro invasion and the expression level of @entity7 and @entity1476 in @entity43 cells, and to evaluate the correlation of expression of @entity7 and @entity1476 in the @entity43 @entity3 . METHOD: Boyden chamber in vitro invasion assay was used to detect the invasive capacity in vitro in two @entity43 cell lines CaOV-3 and COC1. The expression levels of @entity7 and @entity1476 in CaOV-3 and COC1 were evaluated by semiquantitative RT-PCR and Western blot. The activity of @entity1476 in two cell lines was detected by substrate zymography. RESULTS: Boyden chamber in vitro invasion assay indicated that the mean invasion percentage of @entity1 cells (21.9 +/- 1.5) was significantly higher than that of COC1 cells (8.8 +/- 0.9) (P < 0.05). Contrast to COC1 cells, @entity1 cells expressed significantly higher levels of mRNA of @entity7 and @entity1476 as well as protein by RT-PCR and Western blot. Substrate zymography showed that the @entity1476 activity of @entity1 cells was two times than that of COC1 cells. CONCLUSION: The in vitro invasive ability of @entity43 cells appeared to be positive correlated to high expression of @entity7 and @entity1476 . There may be relationship between @entity7 and @entity1476 in the process of @entity3 of @entity43 .
