Comparative study on @entity5 cell apoptosis between gastric and intestinal-type @entity1 XXXX .

@entity1 @entity7279 @entity1885 @entity5 @entity7465 @entity469 @entity1977 @entity3 @entity356
Apoptosis of @entity5 cells between the gastric and intestinal-type @entity1 @entity356 were compared in terms of the expression of oncogene @entity7465 and @entity469 , the histological types, the infiltration depth, and lymph node @entity3 . Terminal deoxynucleotidyl transferase-mediated @entity1977 - @entity1885 nick end-labeling (TUNEL) assay was employed to stain apoptotic cells. Histochemical method(AB-PAS) was applied to stain mucus that is neutral or acidic in nature. Immunohistochemical method (SABC) was used to detect expression of @entity7465 and @entity7279 . The results showed that the mean apoptosis index (AI) of total 48 cases was 8.60+/-2.60. AI in the 30 intestinal type cases was significantly higher than that in the 18 gastric type cases (t=4.67, P<0.01). In the 30 intestinal type cases, the spontaneous apoptosis index of @entity7465 negative cases was significantly higher than that of the positive cases (t=7.16, P<0.01). And in the 18 gastric type cases, the same result was found. (t=11.39, P<0.01). The @entity7465 positive ratio in gastric type cases was higher than that in intestinal type cases (chi2=4.68, P<0.05). There is no significant difference in AI between cases of lymph node @entity3 and non- @entity3 cases in intestinal type cases (t=0.26, P>0.05). But in the gastric type cases, a significant difference existed (t=5.87, P<0.01). A significant difference in lymph node @entity3 ratio was found between the two @entity356 types (chi2=4.48, P<0.05). The @entity469 expression ratio in the 30 intestinal type cases was much lower than that in the 18 gastric type cases (t=4.29, P<0.01). AI of 25 @entity7465 -positive cases was much lower than that of the 23 @entity7465 -negative cases (t=7.80, P<0.01). @entity469 positive ratio in the 25 @entity7465 -negative cases was much lower than that in the 23 negative cases. The difference was statistically significant (t=10.90, P<0.01). Except for few cells scattering within the @entity5 nest, most @entity469 positive cells infiltrated in the interstitium around the @entity5 tissue. In the high-AI cases, @entity469 -positive cells increased. And the @entity469 -positive cells decreased in low-AI cases (r=0.96, P<0.01). Logistic regression analysis suggested that among the control variables, only AI was a statistically significant factor in the regression model (chi2=9.64, P<0.01). We concluded that (1) the spontaneous apoptosis index in gastric-type cases of @entity356 was significantly lower than that in intestinal type cases; (2) AI in the two types was influenced by the expression of @entity7465 and lymph node @entity3 , but no visible connection was found between AI and the infiltration depth or histological types; (3) in the intestinal type cases, AI and the @entity469 -positive cells increased in @entity7465 -negative cases.
