Diffuse sclerosing variant of XXXX :Major genetic alterations and prognostic implications.

@entity3557 @entity1 @entity8958 @entity23275 @entity5 @entity2621 @entity406 @entity982 @entity74 @entity8497 @entity3 @entity15529 @entity2750
AIM: Diffuse sclerosing variant of @entity3557 (DSV-PTC) is an uncommon variant of PTC, and its prognostic significance remains controversial. The aim of this study was to investigate the major genetic alterations of DSV-PTC and their prognostic implications. METHODS AND RESULTS: We included 37 @entity1 with DSV-PTC who underwent thyroid surgery and had @entity406 -fixed @entity982 -embedded samples. We tested for a panel of genetic alterations including BRAF(V) (600E) , @entity8497 codon 61, HRAS codon 12/13/61, and KRAS codon 12/13 point mutations as well as @entity8958 / @entity2750 , @entity8958 / @entity23275 , and @entity15529 /PPARy rearrangements using reverse transcription real-time PCR. All genetic alterations found on PCR were confirmed by Sanger sequencing. Associations between the identified genetic alterations and clinicopathologic characteristics were evaluated. Among 37 cases of DSV-PTC, 17 were positive for @entity8958 / @entity2750 (46%), 6 for @entity8958 / @entity23275 (16%), and 9 for BRAF(V) (600E) (24%). All mutations/rearrangements were mutually exclusive. The remaining 5 cases had none of the above genetic alterations. DSV-PTC with @entity8958 / @entity23275 rearrangement was associated with @entity5 , including T4 and distant @entity3 (P < 0.05). @entity1 with @entity8958 / @entity23275 showed a higher frequency of persistent disease (P < 0.01). In contrast, DSV-PTC with @entity8958 / @entity2750 was associated with a higher prevalence of disease remission (P < 0.05) and coexistent @entity2621 (P < 0.01). CONCLUSION: Taken together, @entity8958 /PTC rearrangement was the major @entity74 seen in @entity1 with DSV-PTC and @entity8958 / @entity23275 rearrangement was associated with advanced stage at diagnosis and poor clinical outcomes. This article is protected by copyright. All rights reserved.
