Immunochemical identification of antigens of XXXX by means of @entity70722 .

@entity1 @entity70722 @entity3377 @entity6467
A crude extract of @entity6467 was obtained by sonication, centrifugation and dialysis, and analyzed by quantitative immunoelectrophoresis. CIE analysis, using a high titer purified rabbit antibody anti-brucella fraction, showed that the @entity6467 extract contained at least 22 antigens that all moved towards the anode. The antigens of the crude extract able to interact with IgG of @entity1 affected by @entity3377 , were identified by means of @entity70722 using sheep 125I-anti human IgG. Fifteen sera of @entity1 affected by @entity3377 were analyzed by @entity70722 and three (Ag6, Ag12, Ag15) of the 22 antigens were able to bind specific human IgG to their corresponding immunoprecipitates. Ag6 was the only one present after one day of the @entity70722 plate exposure. After treatment of @entity3377 patients with antibiotics for two weeks, the three antigens were still able to bind the specific IgG, after two months of treatment the antigens were reduced to two (Ag6, Ag15), while after ten months only one antigen (Ag6) was still able to bind specific human IgG. The @entity3377 titer determined with common methods gave normal values after ten months of therapy. These results suggest that Ag6 is the most important antigen specific for human IgG. A preliminary determination of the molecular weight range of these antigens was carried out by a combination of size exclusion chromatography and CIE analysis. The Kav values were all above 66 kD.
