Matrix metalloproteinases @entity5379 and tissue inhibitors TIMP-1, -2 expression and secretion by primary @entity1 osteoblast cells in response to @entity702 , XXXX , and @entity5383 ceramics.

@entity1552 @entity1 @entity5379 @entity1476 @entity79 @entity2843 @entity1495 @entity702 @entity10678 @entity5383
Osteogenic properties of bone cells are a key parameter governing osseointegration of implant devices. In this context, osteoblasts have a central role via extracellular matrix synthesis and remodeling that they regulate through different protease activity. In this study, we have analyzed the expression of two matrix metalloproteinases (MMPs): @entity1476 (72 kDa) and @entity1495 (92 kDa) and their specific tissue inhibitors @entity2843 and @entity1552 in primary @entity1 osteoblastic cells. The effect of @entity702 , @entity10678 , and @entity5383 ceramics on the synthesis of these proteases was assessed using reverse transcriptase-polymerase chain reaction, enzyme-linked immunosorbent assay, and zymographic analysis. Our results showed that osteoblasts express @entity5379 mRNA. Furthermore, @entity1476 mRNA expression was decreased by @entity702 and increased by @entity5383 whereas @entity10678 did not have any significant effect. Conversely, @entity1495 mRNA expression was stimulated by @entity702 but decreased with @entity10678 , whereas @entity5383 induced no significant changes. Zymographic analysis has evidenced pro- @entity1476 gelatinolytic activity in all cell populations with time-dependent increase profile; pro- @entity1495 , however, was not detected. Enzyme-linked immunosorbent assay data confirmed the production of @entity1476 and very low levels of @entity1495 . In addition, @entity2843 was secreted in 24-h-cultured cells and increased to maximal level at 48-72 h whereas @entity1552 levels were very low. The interactions between @entity1 osteoblasts and the studied biomaterials altered both @entity5379 and @entity2843 expression indicating that biomaterials may influence @entity79 .
