Metabolomic response to exercise training in lean and diet-induced @entity28 XXXX .

@entity19 @entity1602 @entity1386 @entity28
Exercise training is a common therapeutic approach known to antagonize the metabolic consequences of @entity28 . The aims of the present study were to examine 1) whether short-term, moderate-intensity exercise training alters the basal metabolite profile and 2) if 10 days of mild exercise training can correct @entity28 -induced shifts in metabolic spectra. After being weaned, male C57BL/6J littermates were randomly divided into two diet groups: low fat (LF) or @entity1386 ( @entity1386 ). After 12 wk of dietary manipulation, @entity1386 animals were @entity28 and @entity1602 compared with LF animals. @entity19 from each group were further divided into sedentary or exercise treatments. Exercise training consisted of wheel running exercise (2 h/day, 10 days, 5.64 m/min). After exercise training, animals were rested (36 h) and fasted (6 h) before serum collection. Samples were analyzed by high-resolution one-dimensional proton NMR. Fifty high- and medium-concentration metabolites were identified. Pattern recognition algorithms and multivariate modeling were used to identify and isolate significant metabolites changing in response to @entity1386 and exercise training. The results showed that while exercise can mitigate some of the abnormal patterns in metabolic spectra induced by @entity1386 diet feeding, they cannot negate it. In fact, when the effects of diet and exercise were compared, diet was a stronger predictor and had the larger influence on the metabolic profile. External validation of models showed that diet could be correctly classified with an accuracy of 89%, whereas exercise training could be classified 73% of the time. The results demonstrate metabolomics to effectively characterize @entity28 -induced perturbations in metabolism and support the concept that exercise is beneficial for this condition.
