The effect of XXXX nanofiber orientation on osteogenic responses of human osteoblast-like MG63 cells.

@entity15274 @entity33270 @entity7049 @entity23054 @entity623
In this study, @entity7049 ( @entity15274 )/ @entity23054 ( @entity33270 ) solution was electrospun to fabricate fibrous scaffolds with different fiber orientations. Random and parallel @entity15274 nanofiber alignments were achieved by using a metal plate and a rolling rod as the receiver, respectively. The parallel @entity15274 fibrous scaffolds were further hot-stretched to obtain hyperparallel @entity15274 fibrous scaffolds. The @entity15274 fibrous scaffolds were characterized by fiber diameter, interfiber distance, fiber array angle, water contact angle, morphology and mechanical strength. The tensile strength of hyperparallel nano-fibers was approximately 5- and 14-times the parallel and random fibers, respectively. Osteoblast-like MG63 cells were cultured on the @entity15274 scaffolds to study the effects of fiber orientation on cell morphology, proliferation and differentiation. The cells on the randomly-oriented scaffolds showed irregular forms, while the cells exhibited shuttle-like shapes on the parallel scaffolds and had larger aspect ratios along the fiber direction of the hyperparallel scaffolds. @entity623 ( @entity623 ) activity and collagen I (placeStateCol I) and osteocalcin (OC) deposition exhibited fiber orientation dependence. With an increase in parallelism of the fibers, there was a decrease in @entity623 activity and placeStateCol I and OC production. These results suggest that exploitation of @entity15274 fiber orientation may be used to control osteoblast-like cell responses.
