Antibodies to @entity8277 followed by anti-idiotype. Antibodies to insulin in XXXX with @entity4053 .

@entity1 @entity6 @entity19 @entity8277 @entity4053
A @entity1 presenting severe @entity4053 despite low or normal secretion of insulin was found to have IgM antibodies to the @entity8277 . These antibodies stimulated lipogenesis in fat cells in vitro and competed with insulin for binding to insulin receptors. After treatment with glucocorticoids, the anti-receptor antibodies and the @entity4053 both disappeared, and antibodies to insulin appeared in the patient's serum. The anti-insulin antibodies were isolated by affinity chromatography and were found to inhibit the anti- @entity8277 antibodies that were present earlier. The interaction between the patient's anti-insulin antibodies and his anti-receptor antibodies suggests that these two species of antibodies are related as idiotypes and anti-idiotypes. We also studied the interaction of the hypoglycemic patient's anti-receptor antibodies with anti-insulin antibodies of a @entity6 @entity1 and with anti-insulin antibodies of @entity19 immunized to insulin. The hypoglycemic patient's anti-receptor antibodies were neutralized by the @entity6 patient's anti-insulin antibodies, indicating that anti-insulin antibodies with a common idiotype may arise in both @entity6 and @entity4053 . Moreover, mouse anti-insulin antibodies that interacted with mouse anti-receptor antibodies neutralized the hypoglycemic patient's anti-receptor antibodies. In contrast, mouse anti-insulin antibodies that did not interact with the mouse anti-receptor antibodies did not neutralize the hypoglycemic patient's anti-receptor antibodies. Thus, the human anti-insulin antibodies share an idiotype with a specific class of mouse anti-insulin antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)
