Influence of XXXX adipose-derived stromal cells on Wnt signaling in organotypic skin culture.

@entity1 @entity7763 @entity18142 @entity18609 @entity3546 @entity5741 @entity10467
BACKGROUND: @entity1 adipose-derived stromal cells (hASCs) produce various cytokines. Also, there is a growing opinion that a large proportion of the useful effects of cell therapy may be attributable to the secretion of cytokines. Several reports suggested beneficial effects of hASCs on skin. These include antioxidant activity, accelerated wound healing, whitening effects, and antiaging. We investigated the effect of hASCs on skin Wnt signaling, which is associated with skin regeneration and differentiation. METHODS: Pieces of @entity1 skin were cocultured with hASCs, and 2 chambered transwell culture plates were used to prevent direct contact between hASCs and skin. In the control group, pieces of skin were cultured without hASCs. @entity18142 , @entity18609 , @entity10467 , @entity7763 , and @entity5741 mRNA expressions were quantitatively assessed using real-time polymerase chain reaction. The expression levels of @entity3546 were compared using Western blot and immunohistochemical analyses. RESULTS: The @entity18142 and @entity7763 mRNA expression of cultured skin was positively influenced by the presence of hASCs in culture medium (P<0.05). The total @entity3546 protein level in hASC-cocultured skin was higher than that of the control group. Immunohistochemical staining showed that the @entity3546 -stained area of dermis was larger in the hASC-cocultured group than in the control group, and most of the positively stained cells in the dermis were fibroblasts. CONCLUSIONS: The results of the current study showed that hASCs promoted canonical Wnt signaling in organotypic skin culture through paracrine effects, and the increased Wnt signaling was mainly due to dermal fibroblasts.
