Two distinct non-T helper type 2 interleukin-4+ cell subsets in XXXX as revealed by single-cell cytokine analysis.

@entity8236 @entity2047 @entity19 @entity5168 @entity60 @entity1961 @entity58 @entity71211 @entity6232
We have previously defined four @entity19 @entity1961 + peripheral T cell subsets, fractions (Fr.) I-IV, based on expression of the 6C10 and 3G11 determinants (Hayakawa, K. and Hardy, R. R., J. Exp. Med. 1988. 168: 1825). These subsets also show distinctive levels of other cell surface markers: the two minor subsets, Fr. III and Fr. IV, are both @entity8236 RBlow/-, @entity6232 (Mel-14)- and CD44hi, characteristic of secondary T cells. The patterns and levels of cytokine production by individual cells in each subset were determined by bioassay for @entity2047 / @entity58 or @entity60 production after anti- @entity5168 stimulation. Our data revealed that these four phenotypically defined subsets largely coincide with clusters of cells showing uniform distinctive cytokine profiles, i.e. @entity2047 +/ @entity60 -/ @entity58 - (Fr. I and Fr. II, @entity6232 +), @entity71211 +/ @entity58 + (Fr. III, @entity6232 -), and @entity2047 -/ @entity60 low/-/ @entity58 + (Fr. IV, @entity6232 -). Besides these subsets, an @entity6232 -negative cell subfraction within Fr. II appears to represent a transitional population between the @entity2047 +/ @entity60 -/ @entity58 - stage and the @entity71211 +/ @entity58 + stage. Taken together, these results demonstrate the presence of two @entity58 + secondary T cell subsets with distinct cytokine production patterns, and show that the majority of @entity58 + cells found in healthy adult laboratory @entity19 co-produce @entity60 , and thus are not typical T helper type 2 cells.
