Insights into endosomal maturation of @entity1 holo- @entity69 in the enteric parasite XXXX : essential roles of @entity27341 and @entity4814 in biogenesis of giant early endocytic vacuoles.

@entity5572 @entity1 @entity38148 @entity21219 @entity69 @entity16126 @entity2330 @entity1960 @entity281 @entity4814 @entity27341 @entity68
UNASSIGNED: The pathogenic amoeba @entity2330 is one of the causative agents of health hazards in tropical countries. It causes @entity16126 , @entity1960 and @entity5572 in @entity1 . @entity68 is one of the essential nutritional resources for survival and @entity281 caused by the amoeba. The parasite has developed multiple ways to import, sequester and utilize @entity68 from various @entity68 -binding proteins from its host. In spite of its central role in pathogenesis, the mechanism of @entity68 uptake by the parasite is largely unknown. Here, we carried out a systematic study to understand the role of some of the amoebic homologues of @entity1 endocytic Rab GTPases ( @entity4814 and @entity38148 , @entity27341 and @entity21219 ) in intracellular transport of @entity1 holo- @entity69 by the parasite. Flow cytometry and quantitative microscopic image analysis revealed that @entity4814 and @entity27341 are required for the biogenesis of amoebic giant endocytic vacuoles (GEVs) and regulate the early phase of intracellular trafficking of @entity69 . @entity21219 is involved in the late phase, leading to the degradation of @entity69 in the amoebic lysosome-like compartments. Using time-lapse fluorescence imaging in fixed trophozoites, we determined the kinetics of the vesicular transport of @entity69 through @entity4814 -, @entity27341 - and @entity21219 -positive compartments. The involvement of @entity27341 in the early phase of endocytosis by the parasite marks a significant divergence from its host in terms of spatiotemporal regulation by the Rab GTPases.
