Inhibitory effect of Aristolochia fruit on XXXX isozymes in vitro and in vivo.

@entity1447 @entity2544 @entity3761 @entity4920 @entity625 @entity16506 @entity11243 @entity35 @entity7771
The mature fruits of Aristolochia debilis, known in China by the name, "Madouling" has been popularly prescribed in Asia, particularly in China, to treat a range of conditions including gynaecological problems, @entity625 and wound healing. This study was aimed to evaluate the potential effect of Madouling on the @entity1447 ( @entity1447 ) isozymes in vitro in microsomal fractions and in vivo in @entity35 . The influence of Madouling on CYPs activity was first explored by an in vitro method of estimating levels of four respective metabolites in @entity35 liver microsomes. The results were re-examined in vivo in @entity35 by using a cocktail approach involving the probe drugs @entity2544 , @entity11243 , @entity16506 and @entity3761 . Pharmacokinetics of the four substrates was used to analyze the activities of the targeting isozymes. In vitro study revealed that Madouling decreased the activity of @entity7771 , 3A1 and 2E1. However, no significant influence on @entity4920 was found. These results coincided with those of in vivo study to a great degree except that in vivo estimation the herb didn't inhibit @entity7771 significantly. From the data obtained, Madouling is suggested as a candidate for clinically signi   cant @entity1447 interactions. Drug co-administrated with Madouling may need dose adjustment.
