Temporal sequence of motor disturbances and increased cerebellar @entity367 decarboxylase activity following @entity8250 lesions in adult XXXX .

@entity66 @entity8250 @entity2421 @entity400 @entity367 @entity35 @entity2055
Adult male @entity35 were administered 75 mg/kg of the neurotoxin @entity8250 to produce lesions of the inferior olive-climbing fiber projection to the cerebellum. At selected times ranging from 6 h to 43 days postlesion, @entity35 were evaluated for @entity66 , and @entity367 decarboxylase (GAD) activity was determined in the deep cerebellar nuclei and cerebellar vermis. In the deep nuclei non-monotonic changes in GAD activity were found following climbing fiber destruction. Initially, there was a steady increase in GAD activity which peaked at 38% above control values 14 days postlesion. GAD activity then slowly declined, although it remained significantly above control levels at 43 days postlesion, the latest time point examined. In the vermis, GAD activity was significantly increased at 4 days postlesion (+8%) and remained at approximately this level throughout the experiment. The initial behavioral effects of climbing fiber loss included @entity2421 and @entity2055 with severely reduced mobility. With time, the @entity2055 and @entity2421 decreased and movements such as mud-walking and pivoting developed. As these behaviors diminished, other novel conditions such as movement-associated @entity400 and hopping appeared. These results are discussed in the context of the previously reported effects of climbing fiber lesions on the firing rates of Purkinje cells and deep nuclei cells.
