Multiple cis elements and GATA factors regulate a cuticle collagen gene in XXXX .

@entity71685 @entity2199 @entity63921 @entity71684 @entity71683
UNASSIGNED: The cuticle of the @entity2199 @entity2199 is a specialized extracellular matrix whose major component is collagen. Cuticle collagens are encoded by a large multigene family consisting of more than 150 members. Cuticle collagen genes are expressed in epidermis (hypodermis) and may be stage-specific or cyclically expressed. We identified cuticle collagen genes as transcriptional targets of the @entity63921 TGF-b-related signaling pathway. These studies prompted us to investigate the cis-regulatory sequences required for transcription of one of the target genes, @entity71683 . We generated reporter constructs that reproduce stage- and tissue-specific expression of fluorescent markers. We identify four conserved sequence elements that are required for transcription of reporters. Finally, we provide evidence that @entity71683 expression is controlled by a sequence element containing two GATA sites and by the epidermal GATA transcription factors @entity71684 and @entity71685 . genesis, 2015.    2015 Wiley Periodicals, Inc.
