Assessment of cell proliferation by Ki-67 staining and flow cytometry in fine needle aspirates (FNAs) of reactive @entity3298 and XXXX .

@entity706 @entity5 @entity2108 @entity1893 @entity3298
This study was undertaken to assess cell proliferation in FNAs from a series of 57 @entity1893 ( @entity1893 ) and 11 cases of reactive @entity3298 using Ki-67 staining and flow cytometry (FCM). The results were compared and correlated to the cytomorphological subgrouping according to Kiel classification. The mean percentages of Ki-67 positivity were 16.6% and 61.1% for low and high @entity706 , respectively (P < 0.001). The mean S-phase fraction (SPF) determined by FCM was 4.61% for low grade and 12.9% for high @entity706 (P < 0.001). The figures for Ki-67 positivity and S-phase fraction in reactive @entity3298 were 16.8% and 40%, respectively. We observed a strong correlation in low @entity706 between Ki-67 and SPF. A good correlation was also found in reactive @entity3298 . In high @entity706 , however, with highly scattered Ki-67 and S-phase values, this correlation was lost. In some cases this discrepancy can be explained by a rich admixture of non-neoplastic, non-proliferating cells in aspirates from @entity5 . In addition, the existence of a minor @entity5 cell population of high proliferation such as that in @entity2108 will not be accurately analysed by FCM but is easily assessed by Ki-67 staining. However, the main reason seems to be a high variability between the fraction of cells in S-phase and the total number of cells in G1, S and G2 in individual @entity5 .
