Diagnosis of XXXX Using Molecular Beacon.

@entity1 @entity2954 @entity6368 @entity2953 @entity281
@entity6368 is an important @entity2953 ( @entity2953 ) causing pathogen worldwide. Due to absence of an affordable diagnostic assay, routine screening of @entity2954 becomes impossible in developing countries where @entity281 rates are maximum. Treatment is given on the basis of symptoms alone which leads to spread of @entity281 . Thus, development of a rapid, sensitive, specific, and PCR based visual diagnostic assay suitable for developing countries, required for better disease management, is aimed at in present study. Endocervical swabs were collected from @entity1 visiting gynecology department of various hospitals in Delhi. In-house PCR based assay was developed and modified to visual assay using molecular beacon for end-point detection. It was evaluated against Roche AMPLICOR NG kit and rmp gene. Specificity of beacon was confirmed by competition experiments. Diagnostic test was 98.21% specific and 99.59% sensitive whereas negative and positive predicted value were 99.40% and 98.78%, respectively. We also observed that twice the concentration (2X) of premix was stable at 4  C for 4 months and dry swab samples gave concordant results with that of wet swabs. These features make the test best suitable for routine diagnosis of @entity281 in developing countries.
