A highly efficient XXXX nucellus protoplast system for transient gene expression and studying programmed cell death-related processes.

@entity2569 @entity56823 @entity204 @entity2829
KEY MESSAGE: Conditions for the isolation and transfection of @entity2829 nucellus protoplasts were established. We demonstrated its utilization for protein expression, localization, protein-protein interaction, and the investigation of @entity204 -related processes. Plant protoplasts are an important and versatile cell system that is widely used in the analysis of gene characterization and diverse signaling pathways. @entity204 ( @entity204 ) occurs throughout the life of plants from embryogenesis to fertilization. The @entity2829 nucellus undergoes typical @entity204 during development of the embryo sac. The nucellus protoplast shows potential for use in research of @entity204 -related processes. No studies have reported previously the isolation and transfection of nucellus protoplasts. In this study, conditions for the isolation and transfection of @entity2829 nucellus protoplasts were established. The @entity2829 protoplast system can be used for protein expression, localization, and protein-protein interaction. We applied this system to investigate @entity204 -related processes. Quantitative real-time PCR analysis revealed that transient expression of @entity56823 in the @entity2829 nucellus protoplast increases @entity2569 -protease gene transcript level. In addition, b-glucuronidase and luciferase activity assays showed that @entity56823 could enhance the promoter activities of the @entity2569 -protease gene. Thus, we demonstrated the potential of a highly efficient @entity2829 nucellus protoplast system for transient gene expression and investigation of @entity204 -related processes.
