Protection of trocar sites from @entity1086 implantation by @entity4023 carboxymethylcellulose-based bioresorbable membrane (Seprafilm) in a XXXX model [corrected].

@entity1 @entity5 @entity19 @entity3970 @entity1086 @entity4023
BACKGROUND: The risk of port site metastasis in laparoscopic surgery for @entity5 @entity1 is a problem that has yet to be resolved. We examined the protective effect of a @entity4023 -based bioresorbable membrane (Seprafilm) on @entity5 cell implantation at laparoscopic trocar sites. METHODS: Four 2-mm trocar sites were created in @entity19 , and the peritoneal wounds were covered with different-sized pieces of Seprafilm. The protective effect of Seprafilm on the implantation of @entity3970 (a @entity1 @entity1086 cell line) at the trocar sites was assessed after 7 days. In addition, the effects of @entity4023 and Seprafilm on the growth and motility of @entity3970 were examined in vitro. RESULTS: Seprafilm significantly decreased the incidence of implantation compared with the control group. Histologically, Seprafilm was observed on days 1 and 3, as a sheet of gel that covered the injured peritoneum and muscle layer. In an invasion assay using Seprafilm, no cells were found to infiltrate through the gel sheet. CONCLUSION: Seprafilm protects peritoneal wounds by physically covering the injured peritoneum. Therefore, if Seprafilm were attached to the injured peritoneum after laparoscopic surgery for @entity5 @entity1 , it might reduce port site metastasis.
