Functional expression of receptor activator of nuclear factor kappaB in XXXX cell lines.

@entity5737 @entity589 @entity4047 @entity4666 @entity39 @entity5 @entity588 @entity2936 @entity1836 @entity5298 @entity687 @entity5823 @entity16952 @entity379 @entity8252 @entity978
The malignant Hodgkin and Reed-Sternberg (H/RS) cells of @entity978 ( @entity978 ) express several members of the @entity5 @entity39 factor (TNF) receptor family, including @entity5823 and @entity2936 , and secrete several cytokines and chemokines. However, little is known about what regulates cytokine and chemokine secretion in H/RS cells. Although H/RS cells are predominantly of B-cell origin, they frequently share phenotypic and functional features with dendritic cells (DCs). Previous studies reported that receptor activator of nuclear factor kappaB (NF-kappaB) (RANK), a member of the TNF receptor family, is expressed on DCs, and that @entity5298 ( @entity5298 ) enhances DC survival and induces them to secrete cytokines. This study reports that, similar to DCs, cultured H/RS cells expressed RANK. However, unlike DCs, H/RS cells also expressed @entity5298 . Soluble @entity5298 activated NF-kappaB and induced messenger RNA expression of @entity379 , @entity687 ( @entity687 ), @entity4047 , @entity4666 , @entity8252 , and @entity5737 , in addition to the receptors for @entity4666 , @entity4047 , @entity8252 , and @entity16952 . @entity5298 increased @entity687 and @entity4047 levels in the supernatants of H/RS cell lines, an effect that was blocked by soluble RANK. Furthermore, soluble RANK decreased the basal level of @entity687 in one cell line, suggesting that @entity687 was induced by an autocrine @entity5298 /RANK loop. @entity5298 had no effect on H/RS cell survival in culture, and it did not modulate the expression of @entity588 , @entity1836 , @entity589 , or inhibitors of apoptosis proteins. These data provide evidence of further functional similarities between DCs and H/RS cells. The coexpression of RANK and @entity5298 in H/RS cells suggests that they may regulate cytokine and chemokine secretion in H/RS cells by an autocrine mechanism.
