Expression of the XXXX alpha and beta isoforms in human nasal mucosa and polyp epithelial cells.

@entity480 @entity4738 @entity1127
The lower sensitivity of the inflamed nasal mucosa to glucocorticoids might be related to an increased expression of the @entity4738 ( @entity4738 ) beta isoform. We investigated GRalpha and GRbeta mRNA expression in epithelial cells from nasal mucosa and @entity480 . GRalpha mRNA was at least 1000 times more expressed than GRbeta mRNA in both tissues. GRbeta expression (mean+/-SEM of 10(3) cDNA copies/microg of total RNA) was higher in @entity480 (1.15+/-0.19; n=27; P<0.01) than in nasal mucosa (0.62+/-0.10; n=32). @entity480 with > 3% of inflammatory cells had higher GRbeta levels (1.40+/-0.29; n=16) than both nasal mucosa (P<0.01) and @entity1127 with < or = 3% of inflammatory cells (0.80+/-0.18; n=11; P<0.05). No difference in GRbeta expression was found between nasal mucosa and @entity1127 with < or = 3% of inflammatory cells. GRbeta expression correlated with the inflammatory cell number, especially with mast cells (r=0.50, P<0.0001). There was no difference in GRalpha mRNA expression between nasal mucosa and @entity480 . In summary, GRalpha is far more expressed than GRbeta in both tissues. The increased expression of GRbeta may be related to the presence of inflammatory cells.
