[Suppression of the antigen-specific T cell immune response by co-immunization with the XXXX DNA vaccine and recombinant HBsAg].

@entity97 @entity1956 @entity19 @entity403 @entity688
OBJECTIVE: To explore a new therapeutic strategy against @entity1956 and fulminant @entity1956 , we studied effect of co-immunization with @entity97 DNA and HBsAg on the T cell proliferation reaction. METHODS: We immunized the BALB/ c @entity19 with @entity97 DNA vaccine (pcDS2) plus HBsAg by intramuscular injection. The immunization was performed on week 0, 2 and 4. The anti-HBs(IgG)antibody titer, T lymphocyte proliferation reaction , and the expression of @entity688 and @entity403 in CD3 T cell were detected on week 6. RESULTS: The anti-HBs IgG titer induced by pcDS2 plus HBsAg group was higher than that induced by pcDS2, or HBsAg alone. Compared to @entity19 immunized with pcDS2, or HBsAg alone, the stimulated index (SI) of T cell proliferation induced by the pcDS2 plus HBsAg group tested by MTH methods decreased. Besides, the immune suppression of T cell proliferation response induced by co-immunization group was further confirmed by flow cytometry. Finally, the expression of @entity688 and @entity403 in CD3+ T cell was up-regulated in the co-immunization group significantly. CONCLUSION: The co-immunization of @entity97 DNA vaccine and HBsAg can induce the humoral immune response, but cannot induce antigen specific T cell proliferation reaction. Besides, the immune suppression induced by co-immunization may be correlated with the expression of @entity688 and @entity403 .
