Effect of ultraviolet-B-irradiated donor-specific blood transfusions and peritransplant immunosuppression with XXXX on @entity35 cardiac allograft survival.

@entity35 @entity205 @entity1349 @entity870
We have previously demonstrated that pretreatment of ACI recipients with ultraviolet-irradiated donor-specific blood transfusion (UV-DST) leads to permanent cardiac allograft survival without further host immunosuppression (ACI @entity35 are weak responders to Lewis lymphocytes in mixed-lymphocyte reaction). This study examines the effect of UV-DST and the timing of transfusions on ACI cardiac allograft survival in Lewis recipients with and without the addition of peritransplant @entity205 ( @entity870 ) (20 mg/kg i.m.) given on days 0, +1, and +2 in relation to the time of transplantation. The @entity1349 ( @entity1349 ) of ACI cardiac allografts in Lewis recipients was significantly increased to 33.6 +/- 5.7 days (P less than 0.001) by @entity870 treatment alone as compared to 6.5 +/- 0.5 days survival in control. When DST was given on day -3 combined with @entity870 , graft survival was increased to 42.0 +/- 9.3 days (P less than 0.01), as compared to 5.8 +/- 1.3 days when DST alone was used. When DST was irradiated with ultraviolet B (UV-DST) and administered on day -3 combined with peritransplant @entity870 , the @entity1349 was increased to 68.83 +/- 16.1 days as compared to an @entity1349 of 10.0 +/- 1.0 days in controls treated with UV-DST alone. When UV-DST was given on day -7 and combined with peritransplant @entity870 immunosuppression, the results were similar. However, when UV-DST was peritransplant @entity870 course, 4 of 6 recipients maintained their ACI heart allografts indefinitely (greater than 300 days) in contrast to the effect of UV-DST alone ( @entity1349 of 13.5 days). Third-party (W/F) UV-irradiated blood transfusions were ineffective in prolonging ACI cardiac allografts in Lewis @entity35 , regardless of whether the transfusions were given alone or in combination with peritransplant immunosuppression with @entity870 . In conclusion, these results demonstrate that UV-DST combined with a brief peritransplant immunosuppression with @entity870 induces prolonged heart allograft survival in a histoincompatible, strong responder host, and that such effect is donor specific. The use of UV-DST combined with peritransplant @entity870 immunosuppression offers a promising approach to achieving organ transplant unresponsiveness, and decreased sensitization to the donor blood elements, which eventually may have important clinical implications.
