Extracellular @entity141 Promotes Odontoblastic Differentiation of Dental Pulp Stem Cells Via @entity1841 -Mediated XXXX /8 and @entity1366 Pathways.

@entity8087 @entity1841 @entity34846 @entity3963 @entity12208 @entity32968 @entity7378 @entity25882 @entity141 @entity14877 @entity1366
UNASSIGNED: @entity141 is the main element of many pulp capping materials that are used to promote the regeneration of tertiary dentin, but the underlying molecular mechanism is not clear. In this study, we found that @entity141 increased the expression of the odontoblastic differentiation marker gene @entity25882 and promoted odontoblastic differentiation and mineralization of DPSCs, but inhibited ALP activity. @entity141 increases the expression of endogenous @entity1841 , which activates the @entity12208 /8 pathway and promotes the @entity7378 - @entity8087 and @entity8087 - @entity25882 interaction in DPSCs. Inhibition of @entity12208 /8 with @entity34846 partially blocked @entity8087 activity; however, inhibition of the @entity1841 receptor with @entity32968 nearly fully suppressed @entity8087 activity. These results indicate that @entity141 promotes cell differentiation mainly via @entity1841 -mediated Smad-dependent and Smad-independent pathways. We then determined that the phosphorylation level of @entity1366 , but not JNK or @entity3963 , was significantly increased as a result of @entity141 stimulation. Blockage of @entity1366 was found to inhibit @entity8087 activity, indicating that @entity141 triggers the @entity1366 pathway, which subsequently regulates @entity8087 activity. In addition, inhibition of @entity1366 differentially attenuated the phosphorylation levels of @entity12208 /8 and @entity14877 . Collectively, this study demonstrates that @entity141 activates the @entity1841 -mediated @entity12208 /8 and @entity1366 pathways in DPSCs and that @entity12208 /8 and @entity1366 signaling converge at @entity8087 to control the odontoblastic differentiation of DPSCs. This article is protected by copyright. All rights reserved.
