Involvement of S6 kinase and XXXX mitogen activated protein kinase pathways in strain-induced alignment and proliferation of @entity291 aortic smooth muscle cells.

@entity3446 @entity3960 @entity4285 @entity291 @entity28071
@entity291 aortic @entity3960 ( @entity3960 ) phenotype can be altered by physical forces. This has been demonstrated by cyclic strain-induced changes in proliferation and alignment. However, the intracellular coupling pathways remain ill defined. In the present study, we examined whether the @entity28071 and S6 kinase pathway were involved in the mitogenic and morphological changes seen in SMCs exposed to cyclic strain. We seeded @entity291 aortic SMCs on silastic membranes that were deformed with 150-mmHg vacuum. Cyclic strain induced both alignment and proliferation of SMCs. @entity4285 , a specific inhibitor of @entity28071 , hindered @entity3960 alignment, but not proliferation. @entity3446 , a specific inhibitor of the mTOR-S6 kinase pathway, attenuated strain-induced proliferation, but not alignment. Peak activation of @entity28071 and S6 kinase was 351 +/- 76.9% at 5 min and 363 +/- 56.2% at 60 min compared with static control, respectively (P < 0.05). The results suggest that strain-induced @entity3960 alignment is dependent on activation of @entity28071 , but not S6 kinase. Strain induced @entity3960 proliferation is S6 kinase, but not @entity28071 activation, dependent.
