Dendritic cell maturation induced by delivery of ultraviolet-mediated apoptotic XXXX cell lines.

@entity5 @entity14
BACKGROUND: Dendritic cells (DCs) play a critical role in the immune response. The aim of this study was to investigate apoptotic @entity5 cells as an antigen source for DC maturation. MATERIALS AND METHODS: We compared the efficacy of ultraviolet (UV)-irradiation with that of gamma-irradiation in the induction of apoptosis of @entity5 cells. Phenotypic and functional changes of DCs were analyzed after co-incubation with UV-irradiated @entity5 cells. RESULTS: UV-irradiation (1.8 J/cm2) was a more reliable method of inducing apoptosis than gamma-irradiation (20,000 rad). The expression of costimulatory molecules on DCs was upregulated after co-incubation with UV-irradiated @entity5 cells. When we performed allogeneic mixed lymphocyte reaction assay, UV-irradiated @entity5 cells-pulsed DCs stimulated allogeneic T lymphocytes more efficiently than DCs pulsed with gamma-irradiated cells (HT29, p = 0.0419 and WiDr, p = 0.0076). CONCLUSION: UV-irradiation reliably induces apoptosis in cultured @entity14 cells. DCs mature in function and expression of costimulatory molecules after co-incubation with apoptotic @entity5 cells. The clinical implications warrant further study.
