Efficacy of neuroselective and site-specific nociceptive stimuli of XXXX bladder.

@entity35 @entity5019 @entity2951
OBJECTIVE: To evaluate the organ specificity of sine wave electrical stimulation of the bladder through assessment of the expression of @entity5019 -immunoreactive (IR) cells in @entity35 spinal cord regions. METHODS: A total of 37 female @entity35 were divided into 8 groups: sham stimulation; 5, 250, and 2000 Hz stimulation with 1.5- or 2.0-mA intensity; and a group instilled with @entity2951 in the bladder. Using a recently developed bladder sensory threshold device, sine wave electrical stimulation was applied for 90 minutes to the @entity35 bladder. The spinal cord was harvested after the @entity35 were killed. The @entity5019 -IR cells in the spinal regions of the medial dorsal horn, lateral dorsal horn, dorsal commissure, and sacral parasympathetic nucleus were measured. The distributions of the @entity5019 -IR neurons were compared. RESULTS: The maximal expression of @entity5019 -IR cells, induced by 250- and 5-Hz stimulation of the bladder, was found at L6 of the spinal cord and was significantly greater than that in the control group (P<.01). Stimulation with 2000 Hz did not induce any @entity5019 -IR cells. @entity5019 -IR neurons were predominantly seen in the sacral parasympathetic nucleus region in response to 250-Hz stimulation and in the dorsal commissure region in response to 5-Hz stimulation. The number of positive neurons was similar to the number caused by @entity2951 instillation. CONCLUSION: Frequency-specific sine wave electrical stimulation of the @entity35 bladder induced the expression of @entity5019 -IR cells in a neuroselective manner. The bladder sensory threshold device could be used for exploration of the pathophysiology of diseases with disturbances of the afferent pathway of the bladder.
