[Functions of XXXX periodontal myofibroblast in vitro].

@entity1 @entity2533 @entity1970
OBJECTIVE: To investigate the functions of @entity1 periodontal myofibroblast (MFB) in vitro. METHODS: @entity1 periodontal fibroblast (hPDLFs) was cultured and induced to MFB by @entity2533 ( @entity2533 ). MFB was denoted as the experimental group, whereas the hPDLFs was the control group. The groups were continuously cultured and harvested at 0, 12, 24, 48, and 72 h. The MFB marker a-smooth muscle actin ( @entity1970 ) was examined by immunocytochemistry. The expression of fibronectin (FN) between MFB was examined by immunocytochemistry to detect the MFB contact relationship. The mRNA expression levels of @entity1970 , collagen (Col) I, and Col III were measured by reverse transcription-polymerase chain reaction (RT-PCT) to analyze extracellular matrix secretion. The protein expression levels of @entity1970 and Col I were also assessed by Western blot. RESULTS: The experimental group had significantly higher @entity1970 expression than the control group at 0 h (P < 0.001). A positive expression of FN was found between MFB. The experimental group had significantly higher expression levels of Col I and Col III than the control group at 24 h (P < 0.001). CONCLUSION: @entity1 periodontal MFB presents a continuous, high expression of @entity1970 . MFB could interact through FN. MFB is significantly capable of extracellular matrix secretion.
