[Effects of caspases on cerebromicrovascular endothelial cell apoptosis induced by XXXX ].

@entity1977 @entity8773 @entity519 @entity3460 @entity70871 @entity291 @entity413
AIM: To study the effects of caspases on cerebromicrovascular endothelial cell apoptosis induced by @entity519 in vitro. METHODS: The cultured @entity291 cerebromicrovascular endothelial cells were exposed to NaCN in @entity413 -free medium. Cell viability was determined by @entity3460 staining. Cell apoptosis was defined by terminal deoxynucleotidyl transferase-mediated @entity1977 nick end-labeling (TUNEL) and flow cytometry. The expression of caspase-3 was detected by immunocytochemical method. Four caspase inhibitors were used to validate the effect of caspases on cell apoptosis. RESULTS: @entity8773 in @entity413 -free medium initiated cerebromicrovascular endothelial cell injury markedly and typical apoptotic cells were found in this model. The expression of caspase-3 increased significantly. Four caspase inhibitors decreased the number of injured cells. Selective inhibitor of @entity70871 reduced expression of caspase-3 significantly. CONCLUSION: The results suggest that caspases family plays an important role in cerebromicrovascular endothelial cell apoptosis induced by NaCN and caspase-3 acts on the downstream of @entity70871 in protease cascade action to induce apoptosis.
