Characterization of gene expression of XXXX + cells from normal and myelodysplastic bone marrow.

@entity1 @entity35264 @entity31654 @entity2590 @entity46794 @entity2280 @entity1968
Gene patterns of expression in purified @entity2280 (+) bone marrow cells from 7 @entity1 with low-risk @entity2590 ( @entity2590 ) and 4 @entity1 with high-risk @entity2590 were compared with expression data from @entity2280 (+) bone marrow cells from 4 healthy control subjects. @entity2280 (+) cells were isolated by magnetic cell separation, and high-density oligonucleotide microarray analysis was performed. For confirmation, the expression of selected genes was analyzed by real-time polymerase chain reaction. Class membership prediction analysis selected 11 genes. Using the expression profile of these genes, we were able to discriminate @entity1 with low-risk from @entity1 with high-risk @entity2590 and both @entity1 groups from the control group by hierarchical clustering (Spearman confidence). The power of these 11 genes was verified by applying the algorithm to an unknown test set containing expression data from 8 additional @entity1 with @entity2590 (3 at low risk, 5 at high risk). @entity1 at low risk could be distinguished from those at high risk by clustering analysis. In low-risk @entity2590 , we found that the @entity1968 -induced gene ( @entity46794 ), the radiation-inducible, immediate-early response gene ( @entity35264 ), and the @entity31654 ( @entity31654 ) were down-regulated. These data suggest that @entity2280 (+) cells from @entity1 with low-risk @entity2590 lack defensive proteins, resulting in their susceptibility to cell damage. In summary, we propose that gene expression profiling may have clinical relevance for risk evaluation in @entity2590 at the time of initial diagnosis. Furthermore, this study provides evidence that in @entity2590 , hematopoietic stem cells accumulate defects that prevent normal hematopoiesis.
