Intraventricular insulin reduces the antinociceptive effect of [ XXXX , NMePhe4, Gly-ol5]enkephalin in @entity19 .

@entity27241 @entity7862 @entity6 @entity19 @entity10755 @entity3190 @entity669
The effects of pretreatment with insulin on the antinociception induced by intracerebroventricular (i.c.v.) administration of the mu-opioid receptor agonist [ @entity27241 , NMePhe4, Gly-ol5]enkephalin ( @entity7862 ) were studied in @entity19 . Intracerebroventricular pretreatment with insulin (1 and 3 mU) for 60 min dose dependently attenuated the antinociception induced by i.c.v. @entity7862 (5.6 ng) in @entity19 . Intracerebroventricular pretreatment with a highly selective @entity669 kinase inhibitor, @entity10755 , at doses of 100 and 300 ng for 10 min, dose dependently reversed the antinociceptive effect of @entity7862 (5.6 ng) in insulin-treated mice. The antinociceptive effect of @entity7862 (5.6 ng, i.c.v.) was significantly reduced in C57BL/KsJ-db/db @entity6 @entity19 compared with that in age-matched control (C57BL/KsJ-db/ + + ) @entity19 . When C57BL/KsJ-db/db @entity6 @entity19 were pretreated with @entity10755 (300 ng), the antinociceptive effect of @entity7862 was significantly increased. These results indicate that @entity669 kinase may be involved in the reduction of @entity7862 -induced antinociception by insulin in @entity19 . Furthermore, the attenuation of @entity7862 -induced antinociception in C57BL/KsJ-db/db @entity6 @entity19 may be due in part to increased @entity669 kinase activity as a result of @entity3190 .
