Adjuvant treatment of pancreatic carcinoma in a clinically adapted XXXX resection model.

@entity1 @entity788 @entity19 @entity5 @entity136 @entity915 @entity3 @entity420
BACKGROUND: The high rate of local recurrence after radical resection of pancreatic @entity420 fosters intensive efforts to develop new approaches for adjuvant treatment. The established animal models show significant limitations in simulating an adjuvant therapeutic setting. For optimal approximation to the clinical situation we therefore improved a @entity19 orthotopic @entity1 xenotransplantation model. METHODS: Subtotal pancreatectomy in @entity19 was performed after orthotopic inoculation of @entity1 @entity788 cells and manifestation of solid @entity5 . The natural course of disease, @entity5 growth and @entity3 were analysed. @entity136 as a cytotoxic drug was tested in vitro on the cell line used in this model and the effect of adjuvant treatment with @entity136 in vivo was investigated. RESULTS: All @entity5 -resected animals showed local recurrence. Organ @entity3 occurred in 67% in resected compared to 25% of non-resected animals. @entity136 in vitro was ineffective, but as adjuvant monotherapy resulted in a highly significant @entity5 weight and metastatic events. CONCLUSION: Subtotal pancreatectomy for xenotransplanted @entity788 in @entity915 beige @entity19 is feasible. Due to high rates of local recurrence and increased organ @entity3 , this model offers a relevant option for preclinical adjuvant testing, especially as in vitro and in vivo effects of cytotoxic drugs differ enormously.
