Differential distribution of binding sites for 125I-insulin-like growth factor II on trophoblast membranes of XXXX term placenta.

@entity1 @entity442 @entity5100
The syncytiotrophoblast, which is delineated by two polar membranes (the microvillous and the basal plasma membranes), is the main placental structural element controlling maternal-fetal exchanges. These studies of the full-term placenta were undertaken in order to determine whether the microvillous membranes, which are bathed by the maternal intervillous circulation, and basal plasma membrane, which lines the fetal blood capillaries, have binding sites for @entity5100 . The microvillous and basal plasma membranes were purified and found to bind 125I- @entity5100 with significantly different (p < 0.0001) Kd (0.51 and 1.02 nM, respectively). There were more available binding sites in the microvillous (4.4+/-0.3 pmol/mg protein) than in the basal (2.7+/-0.4 pmol/mg protein) plasma membranes (p < 0.0001). Both membranes contained three major (250, 135, and 130 kDa) 125I- @entity5100 /binding-site protein complexes as determined by affinity cross-linking and PAGE. The 250-kDa band (type 2 IGF receptor) was the main band in the basal plasma membranes (46% total bound 125I- @entity5100 ). The 135-kDa band (insulin-receptor alpha subunit) was the main one in the microvillous membranes (48% total bound 125I- @entity5100 ). The amounts of 130-kDa band (type 1 IGF-receptor alpha subunit) in the two types of membranes were similar (30% total bound 125I- @entity5100 ). Only @entity5100 displaced 125I- @entity5100 from the 250-kDa band, while 125I- @entity5100 bound to the 135-kDa band was displaced by insulin, and ligand bound to the 130-kDa band was displaced by @entity442 . Thus there are IGF receptors in both types of membranes of syncytiotrophoblast in the @entity1 full-term placenta, and the distributions of the IGF and insulin receptors are asymmetrical. This may reflect the fact that they face and interact with two independent, different media. Maternal IGF may influence the syncytiotrophoblast by binding to receptors on the microvillous membranes, while fetal IGF may also influence syncytiotrophoblast functions by activating receptors in the basal plasma membranes.
