Assessment of the clinical relevance of 17q25.3 copy number and three-dimensional telomere organization in non-small XXXX @entity1 .

@entity1 @entity1423 @entity470 @entity957 @entity1149 @entity2 @entity1017 @entity420
PURPOSE: To identify potential biomarkers that may provide new therapeutic targets or prognostic indicators for @entity1149 ( @entity1149 ), we investigated the three-dimensional (3D) organization of telomeres and cytoband 17q25.3 copy number in @entity1149 tissues. METHODS: @entity1149 paraffin-embedded tissue specimens from 18 @entity1 were assessed for 3D telomere organization by 3D nuclear telomere imaging followed by quantitative analysis. @entity1 were stratified by smoking, histology, and @entity470 status. Cytoband 17q25.3 was examined by fluorescent in situ hybridization. Data from comparative genomic hybridization and/or single nucleotide polymorphism arrays for cytoband 17q25.3 were obtained and correlated with Q-FISH and 3D telomere results. RESULTS: 3D telomeric profiling demonstrated that the smokers, @entity470 -negative, and @entity957 subgroups tended to have higher numbers of lower-intensity telomeres, indicative of shorter telomeres, as well as higher numbers of @entity1423 compared to non-smokers, @entity470 -positive, and @entity420 , respectively. Gains of cytoband 17q25.3 in conjunction with an increase in the control region 17p11.2 were observed in 7 of 18 (38.9  %) @entity1 , reflecting a @entity1017 . Clonal gains of cytoband 17q25.3 were observed in 11 of 18 (61  %) @entity1 , highlighting a potential biological significance for the genes in this region in @entity1149 tumourigenesis. CONCLUSIONS: The 3D telomere profiles may differentiate @entity1149 @entity1 with different histologies, @entity470 , and smoking statuses, rendering them a potential biomarker for distinguishing these clinically relevant histological and molecular subtypes of @entity2 . Highly frequent clonal gain of cytoband 17q25.3 was also demonstrated, suggesting an important biological role for the genes in this region.
