Trifluoroacetylated adducts in spermatozoa, testes, liver and plasma and XXXX induction in @entity35 after subchronic inhalatory exposure to @entity1508 .

@entity1508 @entity13799 @entity11920 @entity35 @entity11921
The induction of @entity11920 in testes and liver and the presence of trifluoroacetylated ( @entity13799 ) adducts in spermatozoa, testes, liver and plasma were investigated in @entity35 subchronically exposed by inhalation to @entity1508 (15 ppm/4 h/day/5 days/week/9 weeks). After @entity1508 exposure, @entity11921 hydroxylase (p-NPH) activity increased 3.2-fold and @entity11920 apo-protein content 7-fold in testes, whereas in liver, p-NPH increased 2.3-fold and @entity11920 apoprotein content 1.4-fold. These results suggest a differential inductive effect of @entity1508 on @entity11920 in these tissues. Moreover, @entity13799 adducts were present in microsomes of testis and liver and in plasma of @entity1508 -treated @entity35 . The immunoblot analysis of testicular microsomes showed two intense @entity13799 protein bands of 63 and 59 kDa, whereas in liver three intense bands of 100, 76 and 63 kDa were observed. Bands of similar molecular weights to those observed in liver were detected in the plasma of @entity1508 -treated animals. In addition, @entity13799 adducts were detected by immunofluorescence in spermatozoa, probably in the acrosome and/or perinuclear theca region, and in the distal tail of spermatozoa. The increase in @entity11920 apoprotein and p-NPH activity observed in testis and liver microsomes suggests that @entity1508 induces its own biotransformation both hepatically and extrahepatically and in addition, that the nature of the @entity13799 adducts will depend on the proteins present in each tissue. Also, the presence of @entity13799 adducts in spermatozoa may result from the activation of @entity1508 in the reproductive tract. The detailed mechanism of @entity13799 adduct formation and its consequences on the spermatozoa function remain to be fully clarified.
