Gonadotropin-releasing hormone induces @entity24341 and XXXX to regulate cellular morphology and migration in immortalized LbetaT2 pituitary gonadotrope cells.

@entity23422 @entity101 @entity31611 @entity6753 @entity24341 @entity1176 @entity16283 @entity16815
@entity1176 is central to the regulation of reproductive function. It acts on pituitary gonadotropes to stimulate LH and FSH synthesis and secretion. We had previously presented evidence for translational control of @entity6753 synthesis; therefore we investigated whether micro-RNAs might play a role in @entity1176 regulation in LbT2 cells. We show here that @entity1176 strongly induces the AK006051 gene transcript that encodes two micro-RNAs, @entity24341 and @entity31611 , within the first intron. We show furthermore that the AK006051 promoter region is highly @entity1176 responsive. We verify that the @entity16815 Rho GTPase activating protein (GAP) is a target of @entity24341 /212 and show that @entity1176 treatment leads to a decrease in mRNA and protein expression. This reduction is blocked by an anti- @entity16283 to @entity24341 /212 and mimicked by a pre- @entity24341 . @entity1176 inhibits @entity23422 expression through a @entity24341 /212 response element within the 3'-untranslated region. The loss of @entity23422 expression leads to activation of Rac and marked @entity101 and length of neurite-like processes extending from the cell. Knockdown of @entity23422 by small interfering RNA induces the same morphological changes observed with @entity1176 treatment. In addition, loss of @entity23422 causes an increase in cellular motility. Our findings suggest a novel pathway regulating long-term changes in cellular motility and process formation via the @entity1176 induction of @entity24341 /212 with the subsequent down-regulation of @entity23422 .
