Multiparameter flow cytometry provides independent prognostic information in @entity1 with suspected @entity2590 : A study on 804 XXXX .

@entity1 @entity2590 @entity1349 @entity3012
UNASSIGNED: Diagnosis of @entity2590 ( @entity3012 ) relies on well-defined cytomorphologic criteria but is challenging in a significant number of @entity1 . The detection of aberrant antigen expression by multiparameter flow cytometry (MFC) is considered a promising tool to improve @entity3012 diagnostics. We prospectively analyzed 804 unselected @entity1 sent with suspected @entity3012 for correlation of MFC findings with @entity1349 ( @entity1349 ) in the context of cytomorphologic and cytogenetic findings. @entity1 with evidence of @entity3012 by MFC had a significantly worse @entity1349 as compared to those without ( @entity1349 at 2 years, 71.2% vs. 89.4%; P   <   0.001). The number of aberrantly expressed antigens as a continuous variable was significantly associated with @entity1349 [P   <   0.001, hazards ratio (HR): 1.19 per additional aberrantly expressed antigen]. Multivariate analysis proved a diagnosis of @entity3012 by MFC to be independently associated with @entity1349 (P   =   0.050; HR: 1.42). Furthermore, a diagnosis of @entity3012 by MFC was related to inferior survival within all three cytomorphologically defined subgroups, i.e., proven @entity3012 ( @entity3012 , 45.4 vs. 52.8 months, P   <   0.001), suspected @entity3012 (2-year- @entity1349 , 75.0% vs. 82.8%; P   =   0.062), and @entity3012 excluded (2-year- @entity1349 , 63.5% vs. 92.8%, P   =   0.020). Our data clearly demonstrate that, in the assessment of cytopenic @entity1 with suspected @entity3012 , a diagnosis of @entity3012 by MFC is independently associated with @entity1349 , which had been shown in previous studies for today's standard diagnostic parameters cytomorphology and cytogenetics. MFC may, therefore, be considered an additional tool in the diagnostic workup of @entity1 with suspected @entity3012 .    2015 International Clinical Cytometry Society.
