Immunohistochemical characterization of a set of monoclonal antibodies to human XXXX .

@entity6012 @entity4153 @entity1911 @entity5 @entity1577 @entity4292 @entity406 @entity11 @entity4236 @entity3508 @entity195 @entity1823 @entity10470 @entity259 @entity528 @entity44 @entity774
This paper describes the immunohistochemical staining properties of four monoclonal antibodies (MAbs) ( @entity1577 , EB, @entity195 , and KB) which had been previously shown to be specific for purified @entity3508 ( @entity3508 ) by a solid-phase radioimmunoassay. In this study, the authors immunostained a spectrum of normal and neoplastic neuronal, "neuroendocrine," and nonneuronal tissues fixed in @entity406 and embedded in paraffin. Positivity was generally restricted to normal neuronal structures and @entity528 , including @entity774 , @entity6012 , @entity4236 , @entity1911 , @entity10470 , duodenal @entity1823 , and @entity44 with neuroepithelial components. Three staining patterns of the normal or neoplastic neuronal structures were observed: two MAbs ( @entity1577 and EB) stained predominantly the nerve fibers (axoplasm); one ( @entity195 ) stained predominantly the cell bodies (perikaryon); and one (KB) stained both the axoplasm and the perikaryon. "Neuroendocrine" @entity5 such as @entity4153 , @entity4292 , @entity259 , and @entity5 from various locations showed a variable staining pattern. @entity5 cells undergoing mitotic division were usually positive regardless of type. Normal structures other than neuronal or "neuroendocrine," including normal glial cells, were negative. The authors also studied a range of @entity11 with MAbs @entity1577 and @entity195 as well as with Dako polyclonal antiserum to @entity3508 . The results showed that @entity1577 stained the axonal fibers in the normal white matter surrounding these @entity5 ; it did not stain the @entity5 cells or the perikarya of neurons in the surrounding normal gray matter. @entity195 stained the @entity11 cells as well as the perikarya and dendrites of neurons in the surrounding normal gray matter; it did not stain the axonal fibers in the surrounding normal white matter. By contrast, the polyclonal antiserum stained all of these structures. The high degree of staining specificity of the MAbs should prove them to be valuable in immunohistochemical diagnosis of @entity5 as well as in further understanding the role of @entity3508 in neuronal differentiation.
