T-9: LEUKOCYTES MEDIATE THE CROSSTALK BETWEEN THE COMPLEMENT AND COAGULATION SYSTEM IN POLYTRAUMA XXXX .

@entity1 @entity2289 @entity1003 @entity1230 @entity9820 @entity715 @entity130
INTRODUCTION: Severe tissue @entity130 leads to an early excessive activation of the complement and the coagulation system which is associated with a poor outcome. Besides extensive interactions between the two cascades on a fluid-phase level, studies suggest that key molecules in coagulation are expressed on leukocytes. Therefore, we examined the potential crosstalk between complement and coagulation on the leukocyte surface, and aimed to identify the involved molecular mechanisms especially after @entity1230 . METHODS: A prospective clinical study was conducted in @entity1 after severe @entity130 (ISS>= 32) and healthy volunteers. The study was approved by the Independent Local Ethics Committee of the University of Ulm. Blood was obtained upon admission to the ER and 4h, 12h, 24h, 48h, 120h and 240h after @entity130 . Key players of complement and coagulation on the leukocyte cell surface were analyzed by flow cytometry in comparison to expression profiles in healthy volunteers. RESULTS: All leukocytes showed significantly increased expression of @entity2289 during later time points after @entity130 , possibly accounting for decreased fibrinolysis and enhanced risk of @entity715 . TCC was significantly incorporated in monocyte membranes at increased amounts early after @entity130 , returning to base line levels on days 5 and 10. A similar expression pattern was observed for @entity9820 , an essential regulator of protein C activation. TCC levels on monocytes positively correlated with @entity9820 expression and negatively with platelet counts. CONCLUSION: The data are indicative of so far unknown interactions between complement and coagulation on a cellular level and the potential role of leukocyte surface molecules in the development of @entity1003 after @entity130 .
