Onset of activity of fluralaner (BRAVECTO  ) against Ctenocephalides felis on XXXX .

@entity235 @entity24723 @entity11101
BackgroundFluralaner (Bravecto  ) is a novel systemic insecticide and acaricide that provides long persistent antiparasitic activity following a single administration at the minimum dose of 25  mg/kg body weight.MethodsThree negative controlled, randomized studies were conducted in @entity235 to evaluate the start to kill (1 study) and the speed of @entity11101 kill (2 studies) of fluralaner. All @entity235 were infested prior to treatment with unfed adult @entity24723 @entity11101 . @entity235 in the treated groups were administered once orally with fluralaner at a minimum dose of 25  mg/kg body weight, while @entity235 in the control groups were not treated. Separate control and treatment groups were paired at each time point of @entity11101 assessment. Flea counts were performed by combing @entity235 at either 0.5, 1, 2, or 4  hours after fluralaner treatment to measure the start to kill. To evaluate the speed of @entity11101 kill over 12  weeks, @entity11101 counts were performed by combing @entity235 at either 4, 8, 12, or 24  hours after fluralaner treatment and then at 4, 8, 12, or 24  hours after each @entity11101 performed at 4, 8, and 12  weeks following treatment.ResultsIn the start to kill study, the fluralaner activity against @entity11101 started already at 1  hour post-treatment (8% numerical efficacy). At 2 and 4  hours post-treatment, the @entity11101 reduction was significant with 36.7% and 88% efficacy, respectively.In the speed of kill studies, the efficacy against @entity11101 after fluralaner treatment was 80.5% at 4  hours and remained      99.4% at 8, 12 and 24  hours. After @entity11101 in weeks 4, 8 and 12, the efficacy at 4  hours was 96.8, 91.4, and 33.5%, respectively. Efficacy at 8, 12 and 24  hours after @entity11101 was 98.0-100% for the 12  weeks of the study. Except for 4  hours after the 12-week @entity11101 re-infestation, @entity11101 reduction was significant for all time points after @entity11101 re-infestation.ConclusionsSingle oral fluralaner administration rapidly eliminates existing @entity11101 and provides excellent protection against @entity11101 over 12  weeks following treatment.
