Correlation of high-resolution magic angle spinning proton magnetic resonance spectroscopy with histopathology of intact XXXX @entity1313 specimens.

@entity1 @entity808 @entity8580 @entity1313 @entity5 @entity908 @entity16 @entity1653 @entity816 @entity463
High-resolution magic angle spinning proton (HRMAS 1H) magnetic resonance spectroscopy produces well-resolved spectra of metabolites from intact tissue specimens. Here we report the results of a preliminary study of 19 @entity1 @entity1313 obtained by applying this method. Among these 19 cases were 2 low-grade @entity816 , 1 @entity816 , 8 @entity463 , 6 @entity1653 , and 2 @entity808 . In addition, autopsy @entity1 brain tissues from two subjects without any known @entity16 were used as normal controls. The HRMAS 1H magnetic resonance spectroscopy measurements were performed at 2 degrees C on a 400-MHz NMR spectrometer with a HRMAS speed of 2.5 kHz. From these HRMAS 1H MR spectra, we measured the concentrations of 11 metabolites, the ratios of 15 metabolites (resonances) to @entity908 (at 3.03 ppm), and the spin-spin relaxation time for these metabolites (resonances). Our results indicate that these parameters have the potential to characterize @entity5 types and grades with statistical significance as well as identify @entity5 biochemical characteristics. In particular, we found that compared with metabolite concentrations and metabolite spin-spin relaxation time, the metabolic ratios presented the highest sensitivity in differentiating normal tissue from @entity5 , as well as in distinguishing between @entity5 groups. Of 15 analyzed metabolic ratios, 12 showed statistical significance in differentiating normal tissue from low-grade and @entity816 , 13 showed statistical significance in differentiating normal tissue from @entity463 , 14 showed statistical significance in differentiating normal tissue from @entity808 , and 9 showed statistical significance in differentiating normal tissue from @entity1653 . Moreover, our results strongly indicate that the resonance ratio of @entity8580 (at 4.05 ppm) to @entity908 may help distinguish @entity5 type. Our results suggest that the HRMAS method for intact tissue measurement may function as an adjunct to histopathology and contribute to improved accuracy for @entity1313 diagnoses.
