Eicosanoids in XXXX : potential relevance of neutrophil leukotriene B4 to disease pathophysiology.

@entity1 @entity8285 @entity3458 @entity1426 @entity5837 @entity1196
Neutrophil activation with the release of intracellular granule contents has been observed in @entity3458 ( @entity3458 ). Because @entity8285 ( @entity8285 ), a 5-lipoxygenase metabolite of @entity1426 in neutrophils, is a chemoattractant and enhances neutrophil adhesion to endothelium, we assessed plasma levels of this metabolite in controls (n = 9) and individuals with @entity3458 , SS genotype, both in basal "steady state" (n = 37) and during episodes of vaso-occlusion (n = 10) and @entity5837 (n = 5). Thirteen @entity1 with @entity3458 , SC genotype, in steady state were also studied. Although no significant differences were noted between the control (136 +/- 32 fmol/mL) and SC genotype (177 +/- 83 fmol/mL, P >.15), @entity8285 levels were markedly increased in @entity1 with SS genotype in basal steady state (207 +/- 64 fmol/mL, P <.003) compared with those in controls. Values were further increased during vaso-occlusion (264 +/- 94 fmol/mL) and @entity5837 (363 +/- 124 fmol/mL). These levels were significantly different from measurements taken during steady state (P <.04 and P <.0001, respectively). No correlation was noted between LTB(4) level and total white cell or neutrophil count. Additionally, the significant correlation noted in @entity3458 between increased levels of plasma @entity8285 and soluble @entity1196 (P <.03) reflects neutrophil activation. We also observed an effect of @entity8285 on red cell-endothelial adhesion at concentrations that appear clinically relevant (1-10 pmol/mL) with concomitant up-regulation of mRNA for the endothelial vitronectin receptor. These properties of @entity8285 are relevant to disease pathophysiology, providing further evidence of the contribution of the neutrophil to the proinflammatory and proadhesive phenotype in @entity3458 .
