@entity137 and oxidative stress induced by different metallic nanoparticles on XXXX kidney cells.

@entity1 @entity137 @entity28624 @entity26 @entity2041 @entity9545 @entity3759 @entity1948
BACKGROUND: Some manufactured nanoparticles are metal-based and have a wide variety of applications in electronic, engineering and medicine. Until now, many studies have described the potential @entity137 of NPs on pulmonary target, while little attention has been paid to kidney which is considered to be a secondary target organ. The objective of this study, on @entity1 renal culture cells, was to assess the @entity137 profile of metallic nanoparticles ( @entity3759 , @entity1948 and @entity9545 ) usable in industrial production. Comparative studies were conducted, to identify whether particle properties impact @entity137 by altering the intracellular oxidative status. RESULTS: Nanoparticles were first characterized by size, surface charge, dispersion and solubility. @entity137 of NPs was then evaluated in @entity28624 (glomerular mesangial) and @entity1 (epithelial proximal) cell lines. @entity1948 and @entity9545 NPs significantly increased the cell mortality, in a dose-dependent manner. Cytotoxic effects were correlated with the physicochemical properties of NPs tested and the cell type used. Analysis of reactive @entity26 species and intracellular levels of reduced and oxidized @entity2041 revealed that particles induced stress according to their composition, size and solubility. Protein involved in oxidative stress such as NF-kb was activated with @entity1948 and @entity9545 nanoparticles. Such effects were not observed with @entity3759 nanoparticles. CONCLUSION: On glomerular and tubular @entity1 renal cells, @entity1948 and @entity9545 nanoparticles exerted cytotoxic effects that were correlated with metal composition, particle scale and metal solubility. ROS production and oxidative stress induction clearly indicated their nephrotoxic potential.
