XXXX Resistance Mutations in @entity1534 in Association with Virulence Factors and Antibiotic Susceptibility of the Strains.

@entity868 @entity26122 @entity4581 @entity26121 @entity1534 @entity26123
UNASSIGNED: Antibiotic resistance is the major cause for @entity1534 eradication failure. @entity1534 @entity4581 resistance mutations were evaluated in 84 (82 phenotypically @entity4581 resistant and 2 intermediately susceptible) strains by allele-specific PCR and 3'-mismatched PCR. Many (57.1%) of these strains were @entity868 resistant. Prevalence of cagA(+), cagE(+), vacA s1a, m1, i1, and i2 strains was 76.2%, 58.0%, 82.1%, 35.7%, 50.0%, and 50.0%, respectively. @entity26121 , @entity26122 , @entity26123 , and @entity26121 + @entity26122 mutation rates were 64.3%, 23.8%, 1.2%, and 10.7%, respectively. Strains harboring the @entity26122 mutation showed 5.3-fold higher @entity4581 MIC50 than those harboring the @entity26121 mutation. The @entity26121 mutation alone was 1.7-fold more common in vacA i2 strains compared with vacA i1 strains, while the @entity26122 mutation alone was 3-fold more frequent in vacA i1 strains than vacA i2 strains and 3.1-fold more common in @entity868 -susceptible compared with @entity868 -resistant strains. Briefly, @entity4581 resistance mutations were significantly linked to vacA i allele and @entity868 susceptibility. This is the first report about associations between the @entity26121 mutation and less virulent vacA i2 strains, and between the @entity26122 mutation and more virulent vacA i1 strains. As the 2143G mutation often predicts eradication failure by @entity4581 -based regimens, the results may be linked to the better eradication of more virulent strains compared with the less virulent strains.
