Comprehensive biocompatibility testing of a new XXXX -hA bone cement versus conventional @entity4625 cement in vitro.

@entity4625 @entity1 @entity23548 @entity692 @entity141 @entity623 @entity4653
For more than 50 years @entity4625 bone cements have been used in orthopaedic surgery. In this study attempts were made to show whether cultured @entity1 bone marrow cells (HBMC) show an osteogenetic response resulting in new bone formation, production of extracellular matrix (ECM) and cell differentiation when they were cultured onto polymerized @entity4625 ( @entity4625 )- @entity692 (HA), conventional @entity4625 bone cement being taken as reference. Biocompatibility parameters were collagen-I and -II synthesis, the detection of the osteoblast markers @entity623 ( @entity623 ) and osteocalcin, the number of adherent cells and the cytodifferentiation of immunocompetent cells. Cement surface structure, HA stability in culture medium and chemical element analysis of specimens were considered. Fresh marrow cells were obtained from the @entity1 femora during hip replacement. Incubation time was up to ten weeks. We used atomic forced microscopy (AFM) and @entity4653 ( @entity4653 ) for cement specimen analysis. Fluorescent activated cell sorter (FACS), immunohistochemical staining. @entity4653 and light microscopy (LM) served us to judge the cellular morphology. Products of the extracellular matrix were analyzed by protein dot blot analysis, @entity4653 energy dispersive X-ray analysis ( @entity4653 -EDX) and @entity141 / @entity23548 - detection. HA particles increased the osteogenetic potential of @entity4625 bone cement regarding the cellular production of collagen, @entity623 (AP), the number of osteoblasts and the cellular differentiation pattern in vitro. Both tested cements showed good biocompatibility in a @entity1 long-term bone marrow cell-culture system.
