Enzyme activities in oleaginous XXXX accumulating and utilizing exogenous or endogenous lipids.

@entity6211 @entity1792 @entity28436 @entity5910 @entity1110 @entity855 @entity505 @entity375 @entity9275
The activities of @entity855 : @entity1792 lyase (ACL; EC 4. 1. 3. 8), @entity6211 acetyltransferase (CAT; EC 2. 3. 1. 7), @entity1110 +-dependent @entity9275 dehydrogenase (ICDH; EC 1. 1. 1. 42), @entity28436 ( @entity28436 ; EC 4. 1. 3. 1) and malic enzyme ( @entity5910 dehydrogenase; EC 1. 1. 1. 40) were measured in four oleaginous @entity375 , Candida curvata D, Trichosporon cutaneum and two strains of Rhodosporidium toruloides, grown either to accumulate lipid, or to utilize their own lipid reserves or an exogenous supply of lipid. During lipid utilization, activities of ACL and malic enzyme diminished to low levels; CAT and @entity28436 increased considerably in activity and ICDH activity was slightly increased but catalase (EC 1. 11. 1. 6) diminished in activity in both strains of R. toruloides. In all cases, @entity375 utilizing exogenous lipid showed greater changes in enzyme activities than cells utilizing their endogenous reserves. Electron micrographs of Candida curvata D showed proliferation of peroxisomes in starved cells utilizing their own lipid reserve though peroxisomes were more in evidence when the @entity375 had been grown on exogenous lipid. In Lipomyces starkeyi, which shows only minimal utilization of its stored lipid and furthermore cannot grow on exogenous lipid, only the occasional peroxisome was seen when cells were starved of @entity505 .
