Inflammatory role of two venom components of yellow jackets ( XXXX ): a mast cell degranulating peptide mastoparan and phospholipase A1.

@entity137 @entity19 @entity1421 @entity71451 @entity61 @entity6527 @entity5157 @entity6248
BACKGROUND: Venom sac extract of @entity6248 was toxic in @entity19 when injected intraperitoneally but not toxic when injected subcutaneously. Necropsy showed the @entity137 to be an inflammatory response. METHODS: Venom peptide and protein fractions were tested to identify the inflammatory components. The active components were tested to establish whether they might function as adjuvant for venom protein-specific antibody response. RESULTS: @entity137 required the synergistic action of two venom components, a mast cell degranulating peptide mastoparan and phospholipase A1. Both components stimulated @entity1421 release from @entity19 peritoneal cells and macrophages. Mastoparan showed a weak activity to enhance IgE and @entity6527 responses to a yellow jacket venom protein Ves v 5 in BALB/c @entity19 . It was not possible to assess the adjuvant activity of phospholipase A1 because of its suppression of Ves v 5-specific response. @entity71451 , a mast cell degranulating peptide from @entity5157 venom, was inactive as an adjuvant for Ves v 5-specific response. CONCLUSION: Yellow jacket venom contains two inflammatory components, mastoparan and phospholipase A1. Our findings suggest that mastoparan can function as a weak adjuvant for @entity61 cell-associated antibody response.
