In-vitro cytocidal effect of water on XXXX cells: The potential role for intraperitoneal lavage during radical cystectomy.

@entity1 @entity5 @entity2421 @entity150 @entity397
INTRODUCTION: We investigate the cytocidal effect of water on @entity150 cells. Intraperitoneal lavage with sterile water is sometimes used during radical cystectomy to lyse @entity5 cells that might have escaped the surgical specimen. The efficacy of this approach at the cellular level is unknown. METHODS: Three @entity150 cell lines of varying grade, RT4, TCCSUP and T24 were exposed to sterile water, and morphological changes were closely observed under microscopy. Changes of cell membrane integrity, cell viability, and cell number of re-incubated cells after water exposure were measured to determine water induced @entity2421 . RESULTS: The low-grade RT4 cells started swelling immediately upon exposure to water followed by @entity397 within 3 minutes. The higher grade @entity1 and @entity1 cells demonstrated limited @entity2421 swelling with significantly less cell @entity397 after 10 minutes. The damage to cell membrane of RT4 cells was evident at 1 minute; only 10.0% of cells were intact at 10 minutes. On the other hand, 41.9% and 77.8% of @entity1 and @entity1 cells were intact at 10 minutes, respectively. Percentage of viable cells at 10 minutes was 2.1    2.3%, 2.3    0.4%, and 16.1    0.6% for RT4, TCCSUP, and T24, respectively. CONCLUSIONS: Cytocidal effect of @entity2421 can be achieved, to varying degrees, by exposing @entity150 cells to water for at least 10 minutes. This in vitro study may have bearing on the effects seen with intraperitoneal lavage using sterile water during radical cystectomy.
