Diagnosis of XXXX using immunoperoxidase methods to demonstrate common @entity981 cells in cerebrospinal fluid.

@entity1 @entity981 @entity693
The immunological phenotype of cells in the cerebrospinal fluid (CSF) was determined with immunoperoxidase techniques. A medium was used which allowed cells to stand at room temperature for 24 h without appreciable loss of cells. From 62 @entity1 a total number of 208 CSF specimens were analysed. It proved possible to perform 4 determinations and a control reaction on 2.5 ml of CSF: cell count, cytology, E-rosetting and staining with a monoclonal antibody, provided that more than 1 cell per mm3 were present. This study focussed on the presence of the common @entity981 marker (cALL, determined with the monoclonal antibody J5). All 21 CSF specimens containing more than 5% cALL positive cells were from @entity1 with an initial diagnosis of common @entity981 , 8 of these samples were considered to be normal and 3 uncertain by standard cytological criteria. Six of the 8 samples which were cytologically normal, were from @entity1 who had clear meningeal involvement at diagnosis, or developed a relapse later. There were no @entity1 who developed a meningeal relapse on cytological criteria that was not detected by immunocytology. In one @entity1 a cytological diagnosis of meningeal relapse was not confirmed by immunocytology, this @entity1 is disease free 2 yr later without cytostatic treatment. Immunoperoxidase methods to detect cALL positive cells in CSF are an invaluable aid to the diagnosis of @entity693 .
