Tensile force on XXXX macrophage cells promotes osteoclastogenesis through receptor activator of nuclear factor  kB ligand induction.

@entity1 @entity5272 @entity3336 @entity604 @entity5298 @entity58827
Little is known about the effects of tensile forces on osteoclastogenesis by @entity1 monocytes in the absence of mechanosensitive cells, including osteoblasts and fibroblasts. In this study we consider the effects of tensile force on osteoclastogenesis in @entity1 monocytes. The cells were treated with receptor activator of nuclear factor  kB ligand ( @entity5298 ) to promote osteoclastogenesis. Then,expression and secretion of @entity3336 were examined. @entity5298 and the formation of osteoclasts during the osteoclast differentiation process under continual tensile stress were evaluated by Western blot. It was also found that -100  kPa or lower induces @entity5298 -enhanced @entity5272 -resistant acid phosphatase activity in a dose-dependent manner. Furthermore, an increased tensile force raises the expression and secretion of @entity3336 elevated by @entity5298 , and is concurrent with the increase of @entity58827 induction and nuclear factor kB activation. Overall, the current report demonstrates that tensile force reinforces @entity5298 -induced osteoclastogenesis by retarding osteoclast differentiation. The tensile force is able to modify every cell through dose-dependent in vitro @entity5298 -mediated osteoclastogenesis, affecting the fusion of preosteoclasts and function of osteoclasts. However, tensile force increased @entity58827 expression. These results are in vitro findings and were obtained under a condition of tensile force. The current results help us to better understand the cellular roles of @entity1 macrophage populations in osteoclastogenesis as well as in @entity604 remodeling when there is tensile stress.
