Determination of XXXX in pharmaceutical formulations by micellar electrokinetic capillary chromatography.

@entity6542 @entity19131 @entity1622 @entity3440 @entity214 @entity23150
A fast and simple micellar electrokinetic capillary chromatographic method was developed for the analysis of @entity23150 in pharmaceutical formulations. The effects of buffer concentration, buffer pH, @entity214 ( @entity214 ) concentration, organic modifier, applied voltage and injection time were investigated. Optimum results were obtained with a 50 mM @entity19131 buffer at pH 8.0 containing 50 mM @entity214 by using a fused @entity3440 capillary (50 microm internal diameter, 72 cm effective length). The sample was injected hydrodynamically for 4 s at 50 mbar pressure and the applied voltage was +30 kV. The detection wavelength was set at 205 nm. @entity1622 was used as an internal standard. The analysis was performed at 25 degrees C and the total run time was 14 min. The method was suitably validated with respect to linearity range, limit of detection and quantification, precision, accuracy, @entity6542 and robustness. The linear calibration range was 5-100 microg mL(-1) and the limit of detection was determined as 1 microg mL(-1). The method developed was successfully applied to the determination of @entity23150 in pharmaceutical formulations. The results were compared with a spectrophotometric method reported in the literature and no significant difference was found statistically.
