Donor pretreatment with XXXX improves early graft function and survival after porcine liver transplantation.

@entity1707 @entity3849 @entity63 @entity1219 @entity648 @entity492 @entity2545 @entity21953 @entity35 @entity99
Kupffer cell depletion by @entity2545 ( @entity21953 ) in @entity35 livers has previously been proven to minimize @entity99 /reperfusion injury after experimental liver transplantation (LTX). In the current study, we evaluated the effects of donor pretreatment with @entity21953 on @entity99 /reperfusion injury, macro- and microcirculation, and endotoxin clearance of the liver in a porcine model of experimental LTX. Two groups of 12 @entity1707 were treated either with intravenous @entity1219 (0.9%; control) or @entity21953 (20 mg/kg). Twenty-four hours after pretreatment, hepatic macrocirculation was quantified by Doppler flowmetry and liver parenchymous microcirculation by implanted thermodiffusion electrodes. The liver grafts were transplanted after 4-6 h of @entity63 in University of Wisconsin (UW) solution. At 1 and 24 h after LTX, the perfusion values were re-evaluated and histology, biochemical ( @entity492 aminotransferase, @entity3849 ) and functional parameters (partial thromboplastin time, prothrombin time, and @entity648 ) were analyzed. Furthermore, endotoxin clearance of the liver was evaluated at all time points. In @entity21953 -treated animals 80% of the Kupffer cells were destroyed, and 24 h after LTX @entity63 /reperfusion injury in treated grafts was significantly lower in comparison to controls, as shown by histology, @entity3849 levels (741+/-490 U/l in controls vs 379+/-159 U/l in treated grafts, P<0.05), survival (67% vs 92%), and enhanced macro- (total transhepatic blood flow [THBF]=112+/-22 ml/min per 100 g in controls vs 157+/-45 ml/min per 100 g in treated grafts, P<or=0.05) and microcirculation (thermodiffusion [TD]=73+/-9 ml/min per 100 g in controls vs 90+/-16 ml/min per 100 g in treated grafts, P<or=0.05). Despite destruction of the macrophage system in the liver, the transhepatic endotoxin gradient of treated livers was enhanced before and 1 h after transplantation (58% in controls vs 85% in treated grafts, P<0.05). Destruction of Kupffer cells of donors by pretreatment with @entity21953 in @entity1707 is effective in preventing liver graft dys- and nonfunction after LTX. Pretreatment with @entity21953 does not diminish but increase hepatic endotoxin clearance.
