Rapid and generic identification of influenza A and other XXXX viruses with mass spectrometry.

@entity14536 @entity10201 @entity66 @entity11317 @entity15
UNASSIGNED: The rapid identification of existing and emerging @entity10201 viruses is crucial in combating outbreaks and epidemics. @entity66 ( @entity66 ) is a rapid and reliable identification method in bacterial diagnostics, but has not been used in virological diagnostics. Mass spectrometry systems have been investigated for the identification of @entity10201 viruses. However, sample preparation methods were laborious and time-consuming. In this study, a reliable and rapid sample preparation method was developed allowing identification of cultured @entity10201 viruses. Tenfold serial dilutions of ten cultures influenza A strains, mixed samples of @entity11317 with @entity14536 or @entity10201 , and reconstituted clinical samples were treated with the developed sample preparation method. Subsequently, peptides were subjected to @entity66 and liquid chromatography tandem mass spectrometry (LC- @entity15 / @entity15 ). The influenza A strains were identified to the subtype level within 3h with @entity66 and 6h with LC- @entity15 / @entity15 , excluding the culturing time. The sensitivity of LC- @entity15 / @entity15 was higher compared to @entity66 . In addition, LC- @entity15 / @entity15 was able to discriminate between two viruses in mixed samples and was able to identify virus from reconstituted clinical samples. The development of an improved and rapid sample preparation method allowed generic and rapid identification of cultured @entity10201 viruses by mass spectrometry.
