Physiological responses in @entity35 fed extracts of endophyte infected XXXX seed.

@entity4315 @entity308 @entity137 @entity1046 @entity1042 @entity3636 @entity18959 @entity3695 @entity35 @entity291
Three trials involving 184 male @entity35 were conducted to identify the biologically active fraction of endophyte-infected @entity18959 seed. In trial 1, seed infected (greater than 95%) with the endophytic fungus Acremonium coenophialum and shown to be toxic to @entity291 , was serially extracted with @entity4315 , @entity1046 , and @entity1042 , respectively. A second sample of this seed was extracted with @entity3636 only. Controls included a solvent treatment (spraying the various solvents on endophyte-free seed and carrying the mixture to dryness), the seed post-extraction, endophyte-infected seed and endo-phyte-free seed. Laboratory chow was mixed with all diets to provide 50% of the mix. Trial 2 examined the same treatments as trial 1 except that the @entity3636 treatment was omitted. Dosages 3 times (equivalent to 22.5 g seed) those used in trial 1 were applied to endophyte-free seed (7.5 g) in an attempt to accentuate any differences. Trial 3 examined the @entity1042 fraction extracted serially, a @entity1042 fraction extracted batchwise, and a water fraction extracted batchwise. Controls included seed residues post-extraction, solvent residue from @entity1042 sprayed on endophyte-free seed, and endophyte-infected seed. Lowered feed intake and weight gains, and @entity308 serum @entity3695 concentrations were used as indicators of @entity137 . Feed intake was lower in all three trials for @entity35 fed the @entity1042 fraction. In all three trials mean serum @entity3695 concentrations in @entity35 fed the @entity1042 extracts were not different from those fed endophyte-infected fescue seed. @entity35 fed the water extract in trial 3 had lower feed intakes than @entity35 fed endophyte-free seed. However, @entity35 fed the seed, post-water extraction, had serum @entity3695 concentrations similar to that of the negative control. Results suggest that the toxic factor(s) may be concentrated in the @entity1042 extract.
