Biochemical and morphological changes in bone marrow mesenchymal stem cells induced by treatment of XXXX with p-Nonylphenol.

@entity1 @entity137 @entity35 @entity170 @entity622 @entity98 @entity3460 @entity460 @entity23842 @entity26864
OBJECTIVES: In previous investigations, we have shown @entity23842 ( @entity26864 ) caused significant reduction of proliferation and differentiation of @entity35 bone marrow mesenchymal stem cells (MSCs) in vitro. In this study, we first treat the @entity35 with @entity26864 , then carried out the biochemical and morphological studies on MSCs. MATERIALS AND METHODS: Proliferation property of cells was evaluated with the help of @entity98 assay, @entity3460 , population doubling number, and colony forming assay. Differentiation property was evaluated with quantitative @entity622 red assay, measurement of alkaline phosphatase (ALP) activity as well as intracellular @entity460 content. In addition; morphological study, TUNEL test, activated caspase assay, and comet assay were performed to evaluate the mechanism of the cell death. RESULTS: The results showed significant reduction in the colony-forming-ability and population-doubling-number of extracted cells when compared to control ones. In addition, it was revealed that the @entity26864 treatment of @entity35 caused significant reduction in nuclear diameter, cytoplasm shrinkage, and induction of caspase-dependent-apoptosis. Also there was significant reduction in ALP activity, intracellular @entity460 content, and intracellular matrix following osteogenic differentiation. CONCLUSION: As MSCs are the cellular back up for bone remodeling and repair, we suggest more investigations to be conducted regarding the correlation between the increasing number of @entity1 suffering from @entity170 and @entity26864 @entity137 . Also, we strongly recommend WHO and local health organization to prevent industries of using @entity26864 in formulation of industrial products which may cause changes in proliferation and differentiation properties of stem cells.
