Prediction of long-term survival by flow cytometric analysis of cellular DNA content in XXXX with advanced @entity43 .

@entity43 @entity1 @entity2007 @entity5 @entity900 @entity189 @entity5805
The prognostic value of cellular DNA content in @entity43 ( @entity189 ) was investigated by flow cytometric analysis of paraffin-embedded @entity5 blocks from 128 previously untreated @entity1 with International Federation of Gynecology and Obstetrics (FIGO) stage III and IV @entity43 entered in a prospective clinical trial of combination v sequential therapy with @entity5805 and @entity900 . Seventy-three percent of @entity5 were @entity2007 and 27% were diploid. Multivariate analysis using a Cox model showed that cellular DNA content (P less than .001) and FIGO stage (P less than .02) were the only significant independent prognostic variables. The median survival was 13 months for @entity1 with @entity5 and 60 months for @entity1 with @entity5 (P less than .0001). Further analysis indicated that the good prognosis associated with @entity5 was limited to @entity1 with @entity5 , all @entity1 with stage IV (spread beyond the peritoneal cavity or liver metastases) disease having a poor prognosis irrespective of ploidy. On pathological review, nine borderline @entity43 (of low malignant potential) were identified, and seven of these were diploid. These @entity5 have an unusually favorable prognosis, despite apparent dissemination within the peritoneal cavity, a paradox which is often difficult to explain using conventional histological criteria. Although the vast majority of @entity5 in this study (93%) were classified as @entity43 , it is possible that some of the @entity1 with @entity5 may have had @entity5 that were predominantly of low malignant potential, thus accounting in part for the prognostic significance of DNA content. By incorporating flow cytometric DNA analysis with careful histopathological assessment, it may be possible to better identify @entity1 with an inherently good prognosis. This assumes particular importance, as the relatively favorable prognosis of @entity1 with @entity43 appears to be independent of the type of treatment.
