Effect of castration on the synthesis of seminal vesicle secretory protein IV in the XXXX .

@entity71259 @entity134 @entity1522 @entity35
The effects of castration on the synthesis (accumulation) of a major seminal vesicle secretory protein ( @entity71259 ) were examined in young adult @entity35 . In vitro incorporation of labeled @entity1522 into @entity71259 by minced tissue was monitored by immunological methods. Castration resulted in a large decrease in the differential synthesis of @entity71259 . A significant decrease in the relative incorporation of isotope into @entity71259 was evident within 3 days of castration, and by 4 weeks relative incorporation dropped some 30-fold. These changes took place in the presence of a large generalized decline in protein synthesis so that incorporation into @entity71259 on an organ basis decreased by over 200-fold. @entity71259 messenger RNA levels were estimated by RNA excess solution hybridization using a cloned cDNA probe. Relative message levels declined after castration in harmony with the declines in @entity71259 synthesis. @entity71259 mRNA was decreased by a relative factor of approximately 20 and an absolute factor of approximately 200 in long-term (40-day) castrates. Accordingly, the seminal vesicle conforms to the general pattern of @entity134 regulated systems in which hormone withdrawal leads to differential decreases in the steady-state pool size for specific mRNAs. The seminal vesicle is unusual, however, in that a prolonged period is required for maximum differential effects to occur.
