Antinociceptive and anti-inflammatory effects of essential oil extracted from Chamaecyparis obtusa in XXXX .

@entity32 @entity1931 @entity39 @entity19 @entity5 @entity117 @entity199 @entity406 @entity635 @entity3733 @entity842 @entity158 @entity35 @entity1996 @entity1543
UNASSIGNED: Essential oil extracted from Chamaecyparis obtusa (EOCO) consists of several @entity199 with anti-inflammatory effects. @entity199 are expected to have an analgesic effect through inhibition of pro-inflammatory mediators. The present study investigated the anti-nociceptive and anti-inflammatory effects of EOCO in animal models of @entity158 . Intraperitoneal injection with EOCO (5 or 10mg/kg), @entity117 (positive control, 300mg/kg), or @entity3733 (negative control) was performed 1h before the nociception tests: @entity1543 -induced writhing response, @entity406 test, and hot plate test in @entity19 , and acidic saline-induced @entity1931 in @entity35 . The expression of pro-inflammatory cytokines and pro-inflammatory enzymes in @entity406 -injected paws was determined by ELISA and western blotting, respectively. Treatment with EOCO significantly reduced @entity1543 -induced writhing and paw-licking time in late response of the @entity406 tests. The anti-nociceptive effect was comparable with @entity117 . However, EOCO did not affect the reaction time of licking of the hind paws or jumping in hot plate test and the mechanical withdrawal thresholds in acidic saline-induced @entity1931 model. @entity406 -injected paws of @entity19 treated with EOCO revealed the down-regulated expression of @entity5 @entity39 factor-a, @entity635 , inducible @entity842 synthase, and @entity1996 , as compared with those of control @entity19 . These data showed the anti-nociceptive and anti-inflammatory effects of EOCO. The @entity158 -relieving effect might be attributed to inhibition of peripheral @entity158 in association with inflammatory response. EOCO could be a useful therapeutic strategy to manage @entity158 and @entity32 .
