Selective inactivation of heterokaryons of XXXX by anaerobiosis.

@entity1522 @entity2227 @entity28869 @entity15706 @entity535 @entity5132 @entity376 @entity492 @entity367 @entity5911 @entity493
Heterokaryons (hets), but not monokaryons of @entity5132 die when grown anaerobically on minimal medium. Their rates of inactivation increase with decreases in growth temperatures from 37 degrees C to 25 degrees C. At 10 degrees C, however, anaerobiosis is not lethal and suppresses the inactivation which normally occurs among hets cultured aerobically at that temperature. Killing of hets by anaerobiosis can be altered significantly by certain exogenously provided @entity1522 or intermediates of oxidative respiration. Aspartic acid alone promotes inactivation whereas @entity493 , @entity367 or @entity376 individually have no effects. However, @entity2227 and @entity376 combined afford slight protection against inactivation while @entity492 and @entity2227 combined, with or without @entity376 , are highly protective: the activity of the @entity492 - @entity2227 combination is completely negated by the addition of @entity493 . Other common @entity1522 have no effects on het responses to anaerobiosis other than the ability, when combined, to relieve the antagonism of @entity493 for the @entity492 - @entity2227 combination. Anaerobic survivals are also enhanced by @entity5911 or @entity15706 , and even more so by a combination of these two intermediates. The resistances to inactivation elicited by the oxalacetate @entity15706 or @entity492 - @entity2227 combinations are not additive. These relationships are interpreted to signify that inactivation of hets by anaerobic growth is largely, if not exclusively, due to depletion of their @entity5911 and @entity15706 contents for @entity535 biosyntheses, and the unique inability of het cells to replenish those @entity28869 upon subsequent return to aerobic conditions. The observations are consistent with previous indications that mitochondria formed by hets are functionally abnormal.
