Laser-assisted optical rotational cell analyzer measurements reveal early changes in XXXX @entity85 induced by photodynamic treatment.

@entity1 @entity26 @entity21181 @entity4341 @entity22143 @entity2142 @entity85 @entity21180
BACKGROUND: The ability to deform is important for circulating RBCs in vivo, and earlier studies showed that this property can objectively be measured in vitro by the LORCA. In this study it was investigated whether photodynamic treatment of @entity1 RBCs (meant to inactivate contaminating pathogens) affects deformability. STUDY DESIGN AND METHODS: WBC-reduced RBC suspensions (30% Hct) were treated with @entity21180 ( @entity21181 ) and red light. Changes in deformability were analyzed by LORCA measurements, in which elongation of the cells is measured at increasing shear stress. The effect of @entity21181 concentration and light dose was determined as well as the interfering effect of two scavengers of reactive @entity26 species, that is, @entity4341 and @entity22143 . RESULTS: Photodynamic treatment with @entity21181 resulted in clear changes in @entity85 . Deformability changes occurred before onset of @entity2142 . Under relatively mild treatment conditions, especially deformability at low shear stress was decreased, whereas deformability changes at high shear stress only occurred under harsher treatment conditions. Inclusion of @entity4341 and/or @entity22143 primarily prevented deformability changes at high shear stress. CONCLUSION: LORCA measurements can effectively be used to detect changes in deformability that are induced by photodynamic treatment of @entity1 RBCs. A change in deformability represents an early marker of RBC damage under these conditions.
