Tm studies of a tertiary structure from the human XXXX delta agent which functions in vitro as a ribozyme control element.

@entity1219 @entity8723 @entity1372 @entity99 @entity1098 @entity1760
Viroids and other circular subviral RNA pathogens, such as the @entity99 delta agent, use a rolling circle replication cycle requiring an intact circular RNA. However, many infectious RNAs have the potential to form self-cleavage structures, whose formation must be controlled in order to preserve the circular replication template. The native structure of delta RNA contains a highly conserved element of local tertiary structure which is composed of sequences partially overlapping those needed to form the self-cleavage motif. A bimolecular complex containing the tertiary structure can be made. We show that when it is part of this bimolecular complex the potential cleavage site is protected and is not cleaved by the delta ribozyme, demonstrating that the element of local tertiary structure can function as a ribozyme control element in vitro. Physical studies of the complex containing this element were carried out. The complex binds @entity1760 ions and is not readily dissociated by @entity1098 under the conditions tested; > 50% of the complexes remain following incubation in 1 mM @entity1098 at 60 degrees C for 81 min. The thermal stability of the complex is reduced in the presence of @entity1372 ions. A DNA complex and a perfect RNA duplex studied in parallel showed a similar effect, but of lesser magnitude. The RNA complex melts at temperatures approximately 10 degrees C lower in buffers containing 0.5 mM @entity8723 and 100 mM @entity1219 than in buffers containing 0.5 mM @entity8723 with no @entity1219 (78.1 compared with 87.7 degrees C). The element of local tertiary structure in delta genomic RNA appears to be a molecular clamp whose stability is highly sensitive to ion concentration in the physiological range.
