Neurons possessing enzymes of @entity935 synthesis in the mediobasal hypothalamus of XXXX . Topographic relations and axonal projections to the median eminence in ontogenesis.

@entity935 @entity14890 @entity35 @entity669 @entity70564
We evaluated the topographic relations between @entity669 hydroxylase (TH)- and/or aromatic @entity14890 decarboxylase ( @entity70564 )-immunoreactive neurons in the arcuate nucleus (AN), as well as between TH- and/or @entity70564 -immunoreactive axons in the median eminence (ME) in @entity35 at the 21st embryonic day, 9th postnatal day, and in adulthood. The double-immunofluorescent technique in combination with confocal microscopy was used. Occasional bienzymatic neurons but numerous monoenzymatic TH- or @entity70564 -immunoreactive neurons were observed in fetuses. There was almost no overlap in the distribution of monoenzymatic neurons, and therefore few appositions were observed in between. In postnatal animals, numerous bienzymatic neurons appeared in addition to monoenzymatic neurons. They were distributed throughout the AN resulting in the increased frequency of appositions. Furthermore, specialized-like contacts between monoenzymatic TH- and @entity70564 -immunoreactive neurons appeared. The quantification of the fibers in the ME showed that there were large specific areas of the monoenzymatic TH-immunoreactive fibers and bienzymatic fibers in fetuses, followed by the gradual reduction of the former and the increase of the latter to adulthood. The specific area of the monoenzymatic @entity70564 -immunoreactive fibers in fetuses was rather low, and thereafter increased progressively to adulthood. The fibers of all the types were in apposition in the ME at each studied age. Close topographic relations between the neurons containing individual complementary enzymes of @entity935 synthesis at the level of cell bodies and axons suggest functional interaction in between.
