@entity72970 act through their coiled-coil domains to enhance survival and replating capacity of XXXX cord blood hematopoietic progenitors.

@entity1 @entity62887 @entity5787 @entity8090 @entity19 @entity4306 @entity11601 @entity72970 @entity36549 @entity2772 @entity6826
Several angiopoietin-like (ANGPTL) molecules have been implicated in enhancement of ex-vivo expansion of @entity19 and @entity1 (hu) hematopoietic stem cells, but there are no reports on hematopoietic progenitor cells (HPCs). We assessed purified recombinant endotoxin-free hu @entity62887 Coiled-Coil (CC), -3, -3CC, -3 fibrinogen-like domain (FLD), -4, -4CC, -5CC, -6 and -7 for effects on proliferation and survival of HPCs from hu cord blood (CB). None of the ANGPTL molecules stimulated CB @entity4306 proliferation, or enhanced or inhibited colony formation of CB @entity4306 stimulated by various growth factors. However, @entity36549 CC significantly enhanced survival of @entity4306 (CFU-GM, BFU-E, CFU-GEMM) subjected to delayed addition of growth factors. Survival enhancing effects of @entity36549 were neutralized by purified anti- @entity36549 , but not by anti- @entity8090 , -6, or -7. ANGPTL-2CC, -3, and -3CC, but not -4, -6, or -7 also enhanced replating capacity of single CB CFU-GEMM colonies, an estimate of the self-renewal capabilities of HPCs, by greater than 2 fold. Effects of at least ANGPTL-3CC may in part be mediated through phosphorylation of ERK. The ANGPTL molecules did not influence ex-vivo expansion of hu CB CD34(+) cells, alone, or in combination with @entity6826 , @entity5787 , @entity11601 , with or without @entity2772 . Thus, among ANGPTL family members, @entity72970 had enhancing activities on @entity1 @entity4306 survival and replating activity, effects requiring the CC domain of the ANGPTL molecules. This information is of relevance to hu @entity4306 regulation.
