Effect of @entity440 upon cell DNA analysis by flow cytometry in primary XXXX .

@entity0 @entity1 @entity5 @entity440
The effect of @entity440 upon cellular DNA ploidy in @entity0 was assessed by flow cytometry (FCM), in a prospective group of 77 @entity1 with primary operable disease. Each had a needle biopsy at the outpatient visit for diagnosis and FCM analysis, and definitive surgery was performed a median of 8 days later. Forty received @entity440 during this period - 40 mg qds loading dose for 24 h, followed by 20 mg daily until the day of operation: 37 @entity1 received no therapy. The DNA histogram from the needle biopsy was compared with that obtained from the resected @entity5 for each individual. There was little change between the pair of histograms from @entity5 from the untreated @entity1 . In those who had received @entity440 the most consistent effect was a marked reduction in the magnitude of the 'tetraploid' peak in tetraploid or near- @entity5 with DNA indices 1.8-2.0. There was little change in diploid or 'other DNA-aneuploid' @entity5 . In @entity5 (DNA index of 2.0) the percentage of nuclei in the diploid S phase was significantly related to the percentage of nuclei in the diploid G2 + M/tetraploid G1 peak (P less than 0.003, unpaired t test). These data suggest that an effect of @entity440 can be demonstrated by FCM upon @entity5 exhibiting a tetraploid or near-tetraploid DNA content. It is possible that tetraploid or near-tetraploid @entity1 mammary @entity5 may be a distinct group of endocrine responsive @entity5 within the overall group of @entity5 , and that the majority are probably derived from the diploid population rather than being a true aneuploid population.
