Partial purification of a novel stimulant of gastric acid secretion from XXXX non-antral mucosa.

@entity3534 @entity6146 @entity10026 @entity235 @entity1626 @entity112 @entity35 @entity16340 @entity72870 @entity934
A novel stimulant of gastric acid secretion was extracted and purified from the non-antral gastric mucosa of the @entity235 stomach and some of its biological properties were examined. Tissue was boiled in water and extracted in 2% @entity6146 . The stimulatory activity was purified by a combination of reverse-phase high pressure liquid chromatography (HPLC) and gel filtration. Fractions were assayed for a stimulation of basal, @entity10026 - and @entity112 -stimulated gastric acid secretion in the anaesthetized @entity35 . Stimulatory activity was eluted from reverse-phase HPLC columns with @entity1626 and its elution from Sephadex G-10 and G-50 columns suggested a molecular weight of 1,000 to 3,000. The highly purified extracts enhanced basal, @entity10026 - and @entity112 -stimulated acid secretion in the @entity35 . A stimulatory fraction was purified which was devoid of immunoreactive @entity16340 and @entity72870 and contained only small amounts of @entity112 . Its chromatographic properties differed from those of @entity112 and @entity934 . On two occasions the stimulant was purified to homogeneity and found to contain @entity3534 . Insufficient pure material was obtained for full characterization. The stimulant has been tentatively called oxyntin.
