Establishment of a cell line from a Japanese @entity1 useful for generating an in vivo model of XXXX .

@entity1143 @entity1 @entity986 @entity5 @entity19 @entity73297 @entity2615 @entity3620 @entity8605 @entity3969
@entity3620 is a @entity5 with increasing incidence. In the present study, we established six @entity2615 cell lines possessing two allele deletions of the @entity986 ( @entity986 ) gene and one allele deletion of the @entity8605 gene, MM16, MM21, MM26, MM35, @entity73297 and MM56, from @entity1143 fluids or surgically resected @entity5 of Japanese @entity1 . MM21, MM26 and @entity73297 cells failed to develop @entity5 in BALB/c- @entity19 following subcutaneous inoculation. MM16 and MM35 cells slowly generated @entity5 at the site of subcutaneous inoculation in BALB/c- @entity19 , but lost the expression of @entity2615 -related markers such as calretinin, D2-40 and @entity3969 1 in the subcutaneous @entity5 . On the other hand, MM56 cells rapidly generated @entity5 with the expression of calretinin and D2-40 in BALB/c- @entity19 following subcutaneous inoculation. In addition, orthotopic implantation of MM56 cells into BALB/c- @entity19 developed diffusely growing thoracic @entity5 by 3 weeks after implantation. Pleural effusions were observed in these @entity19 4 weeks after implantation. @entity5 invaded aggressively into the chest wall 5 weeks after implantation and often metastasized into the lung, rib, peritoneum and pericardial cavity. On the pleural surface, MM56 cells were growing as single or multiple cell layers with the reactive mesothelium of recipient @entity19 . These results indicate that MM56 cells can behave in a manner characteristic of @entity1 @entity3620 in the thoracic cavity of BALB/c- @entity19 . The in vivo model using MM56 cells may be useful for studying the biological behavior of @entity3620 and developing its diagnostic and therapeutic strategies.
