Nuclear position and shape deformation of chromosome 8 territories in XXXX .

@entity1 @entity1124 @entity5 @entity53957 @entity406 @entity189
Cell type specific radial positioning of chromosome territories (CTs) and their sub-domains in the interphase seem to have functional relevance in non-neoplastic @entity1 nuclei, while much less is known about nuclear architecture in @entity189 cells and its development during @entity5 progression. We analyzed the 3D-architecture of the chromosome 8 territory ( @entity53957 ) in @entity189 and corresponding non-neoplastic ductal pancreatic epithelium. Fluorescence-in-situ-hybridization (FISH) with whole chromosome painting (WCP) probes on sections from @entity406 -fixed, paraffin wax-embedded tissues from six @entity1 with @entity1124 was used. Radial positions and shape parameters of @entity53957 were analyzed by 3D-microscopy. None of the parameters showed significant inter-individual changes. @entity53957 was localized in the nuclear periphery in @entity189 cells and normal ductal epithelial cells. Normalized volume and surface of @entity53957 did not differ significantly. In contrast, the normalized roundness was significantly lower in @entity189 cells, implying an elongation of neoplastic cell nuclei. Unexpectedly, radial positioning of @entity53957 , a dominant parameter of nuclear architecture, did not change significantly when comparing neoplastic with non-neoplastic cells. A significant deformation of @entity53957 , however, accompanies nuclear atypia of @entity189 cells. This decreased roundness of CTs may reflect the genomic and transcriptional alterations in @entity189 .
