Detection of XXXX in blood transfusion: a comparative study among real-time PCR, @entity1062 and microscopy: sensitivity of Malaria detection methods in blood transfusion.

@entity66 @entity281 @entity1062 @entity3964 @entity311 @entity204 @entity4759
The transmission of @entity311 by blood transfusion was one of the first transfusion-transmitted infections recorded in the world. Transfusion-transmitted @entity311 may lead to serious problems because infection with @entity3964 may cause rapidly fatal @entity204 . This study aimed to compare real-time polymerase chain reaction (real-time PCR) with @entity1062 ( @entity1062 ) and light microscopy for the detection of @entity3964 spp. in blood transfusion, both in endemic and non-endemic areas of @entity66 in Iran. Two sets of 50 blood samples were randomly collected. One set was taken from blood samples donated in blood bank of Bandar Abbas, a city located in a @entity4759 , and the other set from Tehran, a non-endemic one. Light microscopic examination on both thin and thick smears, RDTs, and real-time PCR were performed on the blood samples and the results were compared. Thin and thick light microscopic examinations of all samples as well as @entity1062 results were negative for @entity3964 spp. Two blood samples from endemic area were positive only with real-time PCR. It seems that real-time PCR as a highly sensitive method can be helpful for the confirmation of @entity281 in different units of blood transfusion organization especially in @entity311 -endemic areas where the majority of donors may be potentially infected with @entity311 parasites.
