@entity4965 -Induced Gene Expression and DNA Methylation Changes in B6C3F1 XXXX Liver.

@entity1 @entity4966 @entity3229 @entity96 @entity19 @entity409 @entity59922 @entity5207 @entity15699 @entity157 @entity4965 @entity15700 @entity15701
@entity4965 ( @entity4966 ), widely used as an organic solvent in the industry, is a common contaminant in air, soil, and water. Chronic @entity4966 exposure induced @entity157 in @entity19 , and occupational exposure in @entity1 was suggested to be associated with @entity96 . To understand the role of non-genotoxic mechanism(s) for @entity4966 action, we examined the gene expression and DNA methylation changes in the liver of B6C3F1 @entity19 orally administered with @entity4966 (0, 100, 500 and 1000 mg/kg b.w. per day) for 5 days. After 5 days @entity4966 treatment at a dose level of 1000 mg/kg b.w., a total of 431 differentially expressed genes were identified in @entity19 liver by microarray, of which 291 were up-regulated and 140 down-regulated. The expression changed genes were involved in key signal pathways including @entity3229 , proliferation, apoptosis and homologous recombination. Notably, the expression level of a number of vital genes involved in the regulation of DNA methylation, such as Utrf1, @entity59922 , @entity15699 , @entity15700 and @entity15701 , were dysregulated. Although global DNA methylation change was not detected in the liver of @entity19 exposed to @entity4966 , the promoter regions of @entity5207 and Ihh were found to be hypo- and hypermethylated respectively, which correlated negatively with their mRNA expression changes. Furthermore, the gene expression and DNA methylation changes induced by @entity4966 were dose dependent. The overall data indicate that @entity4966 exposure leads to aberrant DNA methylation changes, which might alter the expression of genes involved in the @entity4966 -induced @entity409 .
