Operational tolerance induced by pretreatment with donor dendritic cells under blockade of XXXX pathway.

@entity11643 @entity19 @entity8271 @entity14636 @entity11787 @entity4458 @entity1349
BACKGROUND: Dendritic cells can mount immune response as competent antigen presenting cells. Recently, it has been reported that immature dendritic cells induce prolongation of allograft survival. However, the ability of mature dendritic cells to induce operational tolerance is unclear. Therefore, in this study, we examined the ability of splenic mature dendritic cells to induce operational tolerance to fully allogeneic antigens using @entity19 heterotopic heart transplantation model. METHODS: @entity11643 (H2k) @entity19 received i.v. injections with donor splenic dendritic cells or B cells in the absence or presence of monoclonal antibody (mAb) specific for @entity4458 or @entity8271 / @entity11787 2 weeks before transplantation of a C57BL/10 (H2b) heart. RESULTS: When donor dendritic cells were injected i.v. 2 weeks before transplantation, rejection response was accelerated compared with that of naive @entity19 [ @entity1349 ( @entity1349 ) = 7 and 8 days, respectively]. However, when @entity14636 pathway was blocked by anti- @entity14636 ligand mAb, i.v. injection of donor dendritic cells but not B cells induced indefinite graft survival ( @entity1349 >100 and 20 days, respectively). @entity19 treated with anti- @entity14636 ligand mAb alone rejected their grafts with a @entity1349 of 18 days. Intravenous injection of donor dendritic cells and B cells in combination with anti- @entity8271 / @entity11787 mAbs was less effective to induce graft prolongation ( @entity1349 = 9.5 and 13 days, respectively). CONCLUSIONS: Therefore, under blockade of @entity14636 pathway, mature dendritic cells were tolerogens in vivo independent of @entity8271 /86 pathways.
