PAF-receptor in inflammatory versus non inflammatory XXXX epidermis, cell cultures and embryonal cells.

@entity1 @entity1569 @entity5 @entity3765 @entity5567 @entity694
OBJECTIVE: @entity3765 ( @entity3765 ) has been found on @entity1 keratinocytes to bind @entity5567 , a proinflammatory phospholipid. We aimed to study @entity3765 in a range of dermal cell lines and in samples of normal and psoriatic @entity1 skin to learn about its further role in @entity1 . METHODS: @entity3765 was labeled immunocytochemically, histochemically and additionally studied with western blotting in @entity1 keratinocytes, @entity1 fibroblasts, embryonal keratinocytes, @entity5 cell lines and samples of normal and psoriatic @entity1 skin. RESULTS: Keratinocytes from adult and embryonal epidermis of the 20th week of pregnancy showed a low level of labeling for @entity3765 , but 3 +/- 0.05% of plantar keratinocytes from adults were positive as were 4.2 +/- 0.05% of embryonal plantar keratinocytes from the 21st week of pregnancy. In fibroblasts from adult and embryonal epidermis the protein was expressed at low levels. Western blotting revealed @entity3765 positive bands at 67 k.Da in normal @entity1 skin and @entity1569 , in @entity1569 additionally at 45 k.Da. A 68 k.Da band was found in the @entity694 29 (control), and HaCaT cells, in embryonal keratinocytes additionally at 116 k.Da. CONCLUSIONS: @entity3765 seems not to be important for embryonal or adult fibroblasts. In embryonal keratinocytes it is turned on after the 21st pregnancy week in a few cells seen as a band of 67 k.Da and at 116 k.Da, the latter is not found in adult keratinocytes. An additional 45 k.Da band of @entity3765 was found in @entity1569 that might stand for a truncated receptor. In the @entity5 cell line HaCaT and the HT29 @entity694 cell line @entity3765 characterizes the anti-apoptotic effect of this receptor propagating @entity5 proliferation.
