XXXX affects translocation of @entity14349 to the nucleus in @entity958 .

@entity3785 @entity71618 @entity23053 @entity958 @entity14349 @entity30847 @entity40887 @entity10439
Phytohormone @entity3785 (SA) is a crucial component of plant-induced defense against biotrophic pathogens. Although the key players of the SA pathway are known, there are still gaps in the understanding of the molecular mechanism and the regulation of particular steps. In our previous research, we showed in @entity958 suspension cells that @entity10439 , which specifically modulates @entity30847 activity, significantly suppresses the transcription of the pathogenesis related ( @entity40887 ) gene, which is generally accepted as the SA pathway marker. In the presented study, we have investigated the site of @entity10439 action in the SA pathway. We were able to show in @entity958 plants treated with SA that @entity10439 inhibits the transcription of defense genes ( @entity40887 , @entity71618 ). Fluorescence microscopy of @entity958 mutants expressing 35S:: @entity14349 -GFP (nonexpressor pathogenesis related 1) revealed significantly decreased nuclear localization of @entity14349 in the presence of @entity10439 . On the other hand, @entity10439 did not decrease the nuclear localization of @entity14349 in 35S::npr1C82A-GFP and 35S::npr1C216A-GFP mutants constitutively expressing @entity14349 monomers. Mass spectrometric analysis of plant extracts showed that @entity10439 significantly changes the metabolic fingerprinting while @entity23053 had no effect. We found groups of the plant metabolites, influenced differently by SA and @entity10439 treatment. Thus, we proposed several metabolites as markers for @entity10439 action.
