Expression of topoisomerase IIalpha, Ki-67, XXXX , @entity876 , and argyrophilic nucleolar organizer regions in vulvar squamous lesions.

@entity1364 @entity575 @entity5 @entity957 @entity876 @entity982 @entity6068 @entity5436
OBJECTIVE: It was the aim of this study to investigate the expression of topoisomerase IIalpha (topo IIalpha), Ki-67, @entity1364 ( @entity1364 ), @entity876 , and argyrophilic nucleolar organizer region (AgNOR) staining in @entity6068 ( @entity6068 , N = 10), @entity6068 ( @entity6068 , N = 24), @entity5436 ( @entity5436 , N = 26), as well as @entity957 ( @entity957 , N = 22) of the vulva. METHODS: Formalin-fixed, @entity982 -embedded archival tissue sections were immunostained with monoclonal antibodies against topo IIalpha, @entity876 , and @entity1364 , as well as an affinity-isolated prediluted ready-to-use Ki-67 antibody using a standard immunohistochemical method, and stained with a colloid @entity575 solution for AgNORs. Immunostaining was quantitated by determining the percentage of positively staining nuclei in each sample to express the labeling indices (LIs) by counting the immunoreactive nuclei in 1000 epithelial cells per case for each antibody. In each specimen 200 nuclei were examined using a x100 oil emersion lens, and the mean number of AgNORs per nucleus (AC) was calculated. RESULTS: The LIs for topo IIalpha, Ki-67, and @entity1364 as well as ACs increased stepwise from @entity6068 to VCs, @entity6068 , and SCCs. In contrast to @entity1364 LIs and ACs, a consistent correlation in all four groups was found for Ki-67 and topo IIalpha, suggesting that the latter is a proliferation-associated marker in these tissues. @entity876 expression was seen 8.3% of VCs, 30.8% of @entity6068 , and 54.45% of SCCs. @entity876 LIs were not correlated with LIs for topo IIalpha or Ki-67 in SCCs. The LIs for topo IIalpha, Ki-67, @entity1364 , @entity876 , and ACs were not related to @entity5 progression, FIGO stage, or @entity5 grade in SCCs. CONCLUSIONS: This study presents topo IIalpha and Ki-67 as useful proliferation-associated markers of @entity6068 .
