Different subtype strains of XXXX abundantly colonize in southern China.

@entity1 @entity30811
AIM: This study investigates the colonization rate of @entity30811 in the gastrointestinal tracts of @entity1 living in southern China and applies a modified method for the isolation and subtyping of A.  muciniphila strains from faecal samples. METHODS AND RESULTS: Fresh faecal samples were collected and bacterial DNA was extracted from these samples for real-time PCR analysis. Strains were separated using a culture-dependent sPCR-directed method and classified using an enterobacterial repetitive intergenic consensus (ERIC-PCR) DNA fingerprinting method. The colonization rate for the sample population from southern China was 51  74%. We isolated 22 strains from @entity1 faeces. The results revealed that all strains were identifiable as A.  muciniphila with 99-100% identity to the type-strain @entity30811 . ERIC-PCR resulted in grouping of the DNA fingerprints showed that 12 distinct clusters were distinguished with a delineation level of 100%. CONCLUSIONS: Southern China has a high rate of A.  muciniphila colonization and over 12 different subtype strains reside in faecal samples. SIGNIFICANCE AND IMPACT OF THE STUDY: @entity30811 has a beneficial role in @entity1 gastrointestinal tract. These studies provide a better understanding of A.  muciniphila and details of its colonization in the @entity1 gastrointestinal tract.
