Insecticidal Activity of XXXX Extracts from Culture Filtrates of Mangrove Fungal Endophytes.

@entity137 @entity1046 @entity14940 @entity2607 @entity12799
In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude @entity1046 culture filtrate extracts from 110 mangrove fungal endophytes were screened for their @entity137 . @entity137 tests of all extracts against brine shrimp (Artemia salina) larvae were performed. The extracts with the highest @entity137 were further examined for insecticidal activity against Spodoptera litura larvae and acetylcholinesterase (AChE) inhibition activity. The results showed that the extracts of five isolates exhibited the highest @entity137 to brine shrimp at 50% lethal concentration (LC50) values of 7.45 to 10.24 ppm. These five fungal isolates that obtained from Rhizophora mucronata were identified based on sequence data analysis of the internal transcribed spacer region of rDNA as @entity12799 BPPTCC 6036), @entity14940 (strains BPPTCC 6035 and BPPTCC 6038), A. tamarii (strain BPPTCC 6037), and A. versicolor (strain BPPTCC 6039). The mean percentage of S. litura larval mortality following topical application of the five extracts ranged from 16.7% to 43.3%. In the AChE inhibition assay, the inhibition rates of the five extracts ranged from 40.7% to 48.9%, while @entity2607 (positive control) had an inhibition rate of 96.8%, at a concentration of 100 ppm. The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins. The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents. This study highlights the potential of culture filtrate @entity1046 extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.
