Distribution of cholinesterases of XXXX skeletal muscle.

@entity32714 @entity95
Two cholinesterase (ChE) fractions were extracted from homogenates of @entity95 skeletal muscle. One of them the sarcoplasmic ChE could be dissolved quantitatively with solution A. Following the extraction with solution B the myofibrillar ChE fraction could be recovered. The @entity32714 of the sarcoplasmic extract was about 38% and the activity of myofibrillar extract was about 50% of the @entity32714 of muscle homogenates. According to our calculations about 10-12% of the total @entity32714 is insoluble. By the aid of PAGE seven zones of @entity32714 could be distinguished in the sarcoplasmic extract. The activity of myofibrillar ChE-fraction was obtained by chromatographic purification of LMM. The LMM1 proved to be inhomogeneous with PAGE, however, only one fraction displayed @entity32714 . Immune-serum produced against myosin did not give reaction with purified sarcoplasmic ChE, only with the myofibrillar fraction. Our results suggest that the heterogeneous distribution of muscle ChE-es in different molecular forms is not an artifact.
