Renal microRNA- and RNA-profiles in progressive XXXX .

@entity51837 @entity21275 @entity4106 @entity36117 @entity29513 @entity1195 @entity740 @entity2851 @entity3049 @entity25449 @entity45317 @entity9134 @entity71587 @entity11274 @entity65959 @entity10073 @entity8127
BACKGROUND: MicroRNAs (miRNAs) contribute to @entity740 progression via regulating mRNAs involved in renal homeostasis. However, their association with clinical outcome remains poorly understood. MATERIALS AND METHODS: We performed miRNA and mRNA expression profiling on renal biopsy sections by qPCR (miRNA) and microarrays (mRNA) in a discovery (n=43) and in a validation (n=29) cohort. miRNAs differentiating stable and progressive cases were inversely correlated to putative target mRNAs, which were further characterized by pathway analysis using KEGG pathways. RESULTS: @entity36117 , miR-140-3p, miR-532-3p, miR-194, @entity65959 , @entity10073 , and @entity21275 were downregulated in progressive cases. These 7 miRNAs correlated to upregulated 29 target mRNAs involved in inflammatory response, cell-cell-interaction, apoptosis, and intracellular signaling. In particular @entity21275 and miR-532-3p were associated with distinct biological processes via the expression of their target mRNAs: Reduced expression of @entity21275 in progressive disease correlated with the upregulation of target mRNAs participating in inflammatory pathways ( @entity11274 , @entity2851 , @entity29513 , @entity71587 , @entity25449 , @entity1195 , @entity51837 , and @entity4106 ). Progressive cases also showed a lower expression of miR-532-3p and an increased expression of target transcripts involved in apoptosis pathways ( @entity8127 , @entity3049 / @entity3049 , @entity45317 , and @entity9134 ). In the validation cohort we confirmed the decreased expression of @entity21275 and miR-532-3p, and the inverse correlation of these miRNAs with the expression of 9 of the 12 target genes. The levels of the identified miRNAs and the target mRNAs correlated to clinical parameters and histological damage indices. CONCLUSIONS: These results suggest the involvement of specific miRNAs and mRNAs in biological pathways associated with the progression of @entity740 . This article is protected by copyright. All rights reserved.
