Decreased @entity19617 mRNA content and insulin-sensitive deoxyglucose uptake show insulin resistance in the XXXX @entity35 heart.

@entity101 @entity13345 @entity1184 @entity36262 @entity19617 @entity35 @entity413
OBJECTIVES: Insulin resistance in skeletal muscle and adipose tissue often accompanies @entity101 ; however, it has not been shown that heart muscle is similarly affected. The aims of this study were to determine whether basal and insulin-stimulated @entity413 transport and @entity413 transporter mRNA content are altered in the spontaneously @entity101 @entity35 (SHR) heart. METHODS: Hearts from 16-18-month-old SHRs were compared to their normotensive (WKY) controls. The accumulation of @entity36262 (2DG6P), detected using 31P nuclear magnetic resonance spectroscopy, was used to assess @entity413 uptake before and during insulin stimulation in the isolated perfused heart. The mRNA levels of both the insulin-sensitive @entity413 transporter ( @entity19617 ) and the transporter responsible for basal @entity413 uptake ( @entity13345 ) were quantified by Northern blot analysis. RESULTS: The @entity101 @entity35 hearts exhibited @entity1184 in that the heart/body weight ratio was increased by 59%. In these hearts, the basal rate of @entity413 uptake was 3-fold greater and hexokinase activity was 1.6 fold greater than that of the control @entity35 hearts. On exposure to insulin, accumulation of 2DG6P increased 5-fold in the control hearts, but only 1.4-fold in the SHR hearts. Thus, in the presence of insulin, the rate of @entity413 uptake by the @entity101 @entity35 heart was significantly (P < 0.05) reduced, being 82% of control. @entity19617 mRNA content was decreased was no significant difference in the @entity13345 mRNA content. CONCLUSION: We have demonstrated insulin resistance in the hypertrophied heart of the @entity101 @entity35 that may have a molecular basis in a lower @entity19617 content.
