Cationic Liposome- Multi-Walled Carbon Nanotubes Hybrids for Dual siPLK1 and XXXX Delivery In Vitro.

@entity632 @entity20186 @entity5 @entity98 @entity3105 @entity3655
PURPOSE: To formulate f-MWNTs-cationic liposome hybrids for the simultaneous delivery of siPLK1 and @entity632 to @entity5 cells. METHOD: f-MWNTs-cationic liposome hybrids were prepared by the thin film hydration method where the lipid film was hydrated with 100   g/ml or 1  mg/ml of ox-MWNTs- @entity3655 (+) or MWNTs- @entity3655 (+) in 5% dextrose. siRNA complexation and protection ability was determined by agarose gel electrophoresis. f-MWNTs and liposome interaction was evaluated using @entity20186 (NR) fluorescence spectroscopy. Cellular uptake in A549 cells was assessed by flow cytometry. Silencing of target proteins was determined by Luciferase and @entity98 assays. Sub-G1 analysis was performed to evaluate apoptosis following co-delivery of siPLK1 and @entity632 ( @entity3105 ). RESULTS: Zeta potential and siRNA complexation profile obtained for all hybrids were comparable to those achieved with cationic liposomes. ox-MWNTs- @entity3655 (+) showed greater extent of interaction with cationic liposomes compared to MWNTs- @entity3655 (+). ox-MWNTs- @entity3655 (+) was able to protect siRNA from nuclease-mediated degradation. Enhanced cellular uptake of both the carrier and loaded siRNA in A549 cell, were observed for this hybrid compared to the liposomal carrier. A synergistic pro-apoptotic effect was obtained when siPLK1 silencing was combined with @entity632 treatment for the hybrid:siRNA complexes compared to the lipoplexes, in A549 cells in vitro. CONCLUSIONS: f-MWNTs-cationic liposome hybrid designed in this study can serve as a potential vehicle for the co-delivery of siRNA and cytotoxic drugs to @entity5 cells in vitro.
