Detection of XXXX DNA in @entity3137 using in situ DNA-hybridization applied on paraffin sections.

@entity1377 @entity6205 @entity8407 @entity75 @entity5 @entity1185 @entity957 @entity1799 @entity1082 @entity8619 @entity3137
The in situ DNA hybridization technique, carried out under stringent conditions, was used to detect @entity1082 ) DNA of types 6, 11, and 16 in paraffin sections of 32 surgically treated @entity3137 . Expression of HPV structural proteins was analyzed by means of the immunoperoxidase (IP-PAP) method. A total of 10 (31.3%) of the 32 lesions proved to express HPV antigens, which were found in 4 of 7 @entity1185 , in 2 of 2 classic @entity6205 , in 2 of 10 @entity1377 , and in 2 of 3 @entity75 . Two of the @entity1185 contained HPV 6 DNA, and 4 additional lesions were positive for HPV 11 DNA. In one of the @entity6205 , a double infection by HPV 6 and 11 was found, while the second was positive for HPV 11 alone. Both the HPV antigen-positive @entity1377 contained HPV 6 DNA, as did the HPV antigen-positive @entity75 . Of the latter, one was infected by HPV 6 and 11. DNA of the "high-risk" @entity1799 was contained in two lesions: one @entity75 and one of the two @entity957 . The results confirm the previously reported evidence of HPV involvement in @entity3137 . The implications of these findings are discussed in terms of the well-established premalignant @entity8407 and @entity8619 , with special emphasis on the discovery of the "high-risk" @entity1799 in the latter as well as in @entity5 .(ABSTRACT TRUNCATED AT 250 WORDS)
