Evidence for a PCN-P450 enzyme in XXXX and comparison of its development with that of other @entity109 -inducible forms.

@entity17690 @entity3208 @entity2929 @entity1573 @entity619 @entity10045 @entity109 @entity2975 @entity22097 @entity7680 @entity35 @entity5954 @entity1068 @entity4528
Of four monoclonal antibodies to purified @entity35 liver cytochrome P450s, including those from @entity5954 -, @entity109 -, @entity1068 -, and @entity22097 -treated @entity35 , only the monoclonal antibody against @entity22097 -inducible P450 immunodetected proteins in @entity1573 liver microsomes after blotting from @entity3208 - @entity2975 gels. This protein migrated identically with the @entity22097 -inducible P450 detected in microsomes from @entity619 -treated @entity35 . It was most predominant in liver microsomes from @entity1573 at 1 day posthatching, whereas much lower levels were observed in the embryo and at 36 days posthatch. @entity109 and @entity619 were both effective inducers of this protein. The developmental profile and induction by @entity109 and @entity619 of several cytochrome P450-associated catalytic activities were compared with those of the immunodetected protein. @entity1573 liver microsomal erythromycin demethylase, a characteristic activity of @entity35 @entity22097 -inducible P450, was similar in developmental profile and induction to the immunodetected protein, with a high degree of augmentation at 1 day posthatch compared with that in the embryo and at 36 days posthatch; @entity4528 epoxidase, @entity10045 demethylase, @entity7680 demethylase, and @entity17690 demethylase were more similar to each other in development and induction and were less well correlated with the immunodetected protein. This evidence suggests the presence in @entity1573 liver of at least two types of P450, one a form related to the @entity22097 -inducible P450 family. All of the catalytic activities were induced after pretreatment of @entity1573 with @entity109 but @entity4528 epoxidase was most effectively induced. @entity4528 epoxidase was also detected in microsomes from untreated embryos as early as 7 days of incubation. @entity2929 demethylase was the only catalytic activity induced by @entity619 . There was a trend of increased specific activity toward all the substances after hatching, indicating a more efficient P450 system, possibly due to a sharp increase in some isozymes, including the form from the @entity22097 -inducible P450 family. This evidence for a @entity22097 -inducible P450 in @entity1573 agrees with sequence information that suggests the early evolution of this form and demonstrates the suitability of the @entity1573 for studies of P450 evolution.
