Detection of XXXX and z-Drugs in Hair Using an UHPLC-MS/MS Validated Method: Application to Workplace Drug Testing.

@entity3175 @entity42111 @entity1044 @entity1042 @entity71920 @entity68629 @entity17133 @entity1245 @entity1401 @entity11465 @entity71919 @entity2053 @entity5189 @entity2492
BACKGROUND: A sensitive and reproducible Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry method has been developed for the simultaneous quantification of the 29 commonly prescribed @entity1245 and z-drugs in hair. The method was validated according to international guidelines. METHODS: After decontamination (with @entity1044 and water), compounds were extracted from 20 mg of pulverized hair samples using @entity1042 at 45  C and sonication for 2 hours. The drugs were recovered by liquid-liquid extraction using @entity42111 , evaporated to dryness, and reconstituted with 100  L of @entity1042 before injection in the UPLC-MS/MS. RESULTS: The applied gradient ensured the elution of all the compounds within 7 minutes using 0.1% @entity5189 in water and @entity1042 as mobile phase. The lower limit of quantification values ranged from 0.5 to 5 pg/mg of hair. Calibration curves were linear for almost all the compounds and ranged from the limit of quantification to 620 pg/mg hair. The bias and relative standard deviation of the intraday and interday imprecision were lower than 15% in 3 fortified "in-house" quality control samples, 1 external quality control sample, and 1 authentic hair sample (from a @entity3175 user). No significant matrix effects were observed for most of the compounds, and the extraction efficiency of the sample cleanup procedure ranged from 19% to 82% with a relative standard deviation <15% [except for clobazam (16%), @entity68629 (20%), @entity11465 (18%), and @entity71919 (20%)]. The method was then successfully applied to the analysis of 40 hair samples from the workplace drug testing, containing @entity1401 , @entity17133 , @entity2053 , @entity3175 , @entity2492 , and @entity71920 (and metabolites). CONCLUSIONS: The method was completely validated and can be of interest to clinical and forensic laboratories.
