Effects of antibiotics and medium supplements on steroidogenesis in cultured XXXX luteal cells.

@entity801 @entity8788 @entity45916 @entity1420 @entity2379 @entity4259 @entity114 @entity3639 @entity291
Corpora lutea were removed from regularly cycling dairy @entity291 , dissociated with collagenase and cultured for 8 or 10 days in Ham's F-12 medium. In Exp. 1 treatment with @entity8788 , or an @entity8788 - @entity45916 - @entity4259 combination (ITS), increased @entity1420 production from basal levels on Day 4 of culture to 234% (P less than 0.01) above controls on Day 10. LH alone increased @entity1420 production 45% above controls on Day 10 (P greater than 0.05). When LH was combined with @entity8788 or ITS, @entity1420 production was stimulated to an average of 1802% (P less than 0.01) above controls on Day 10 of culture. @entity45916 or @entity4259 without @entity8788 did not allow LH to stimulate @entity1420 synthesis. In Exp. II, LH alone or LH plus @entity801 or @entity114 - @entity3639 increased @entity1420 production from basal levels on Day 2 steadily to an average of 468% (P less than 0.01) above controls (no antibiotics) by Day 8 of culture. The addition of @entity2379 , alone or in combination with the other antibiotics, inhibited all LH-stimulated @entity1420 synthesis, but did not affect basal @entity1420 levels. We conclude that @entity8788 is essential for maximal steroidogenesis in a @entity291 luteal cell culture system, and that LH-stimulated @entity1420 production is inhibited in the presence of @entity2379 , but is not inhibited by @entity801 or @entity114 - @entity3639 . The elimination of @entity2379 , coupled with the addition of @entity8788 to the cell culture system increased the responsiveness of the cells to LH. These culture conditions represent the first report in which LH increased total @entity1420 production for 10 days, maintaining luteal function in a chemically-defined culture system.
