Expression of podoplanin and other lymphatic markers in the XXXX anterior eye segment.

@entity1 @entity7802 @entity18262 @entity3024 @entity14600 @entity469 @entity11527 @entity13712 @entity11274 @entity9350
PURPOSE: To investigate the expression of lymphatic endothelial cell (LEC) markers in tissues of the anterior eye segment. METHODS: Sections of @entity1 anterior segments from eight eyes of eight donors (37-100 years) were stained for @entity7802 , @entity18262 , @entity14600 , @entity13712 , Pcx, @entity9350 , @entity11274 , @entity3024 , and @entity469 . @entity13712 localization was additionally analyzed by immunogold labeling on sections of five eyes. Expression of LEC markers and chemokine receptor/ligands was analyzed by RT-PCR in iris and trabecular meshwork (TM) tissue from three eyes and eight @entity1 TM (hTM) cell cultures. RESULTS: @entity7802 and @entity18262 stained no structures in the anterior segment. @entity14600 stained single dendriform cells in the ciliary body, the TM, and the iris. @entity13712 stained all trabecular cells, the cells of the trabeculum ciliare, and the anteriormost perimysium cells of the ciliary muscle. Schlemm's canal endothelium was not stained but reacted to a podocalyxin antibody. In the iris stroma, single dendriform cells were stained; at the anterior surface, almost all cells were @entity13712 (+). Few stromal cells were @entity13712 (+)/Lyve(+), but several anterior surface cells were @entity13712 (+)/ @entity3024 (+). Solitary @entity9350 (+) cells were observed there, too. IF results were confirmed by PCR, but @entity18262 was detected in TM and iris. Cultured hTM cells displayed partial @entity13712 / @entity3024 colocalization. CONCLUSIONS: Coexpression of @entity13712 and @entity3024 at the anterior iris surface and in the chamber angle suggests the constitution of a chemokine gradient guiding @entity11527 through the anterior chamber. The more pronounced expression of @entity13712 in the TM could favor egression of @entity11527 by way of the conventional outflow.
