Similarities in function between XXXX cells and macrophages and their inhibition by murine monoclonal antibodies.

@entity1 @entity788 @entity137 @entity6931 @entity1334 @entity224 @entity420
Fine structural studies of @entity1 @entity788 have demonstrated their composition of one major secretory cell type, a mucin secreting cell. It was also demonstrated that @entity788 cells have a high endocytotic activity via the regular endocytotic pathway with uptake via coated pits and vesicles. These findings indicate a striking similarity between @entity788 cells and @entity1 mononuclear phagocytes. Therefore monocyte-related functions like endocytosis, lysosomal enzyme secretion and @entity224 anion production were measured in various cell lines of @entity1 @entity788 in comparison to @entity1 mononuclear phagocytes. These functions were measured under basal conditions and after exposure to zymosan or immune complexes. All @entity788 cells tested demonstrated increased endocytotic, secretory and oxidative burst activity to a similar extent as @entity1 mononuclear phagocytes after exposure to the stimuli (zymosan or immune complexes). In a further attempt murine monoclonal antibodies produced against @entity788 cells were characterized in relation to their tissue specificity, the epitope recognized in in vitro and in vivo radiolocalization and their potential to mediate biological functions (e.g. antibody dependent cellular @entity137 or complement mediated cytolysis). Mouse monoclonal IgG1 antibodies which specifically bind to the majority of well differentiated @entity420 of the @entity1 pancreas as well as to @entity1 mononuclear phagocytes have been shown to inhibit several cellular functions (lysosomal enzyme release, pinocytosis of colloidal gold 198Au, and chemiluminescence) of those cells. Incubation of 10 micrograms/ml of monoclonal IgG1 antibody with @entity788 cell lines or @entity1 monocytes either simultaneous or followed by the stimulation with zymosan (50 micrograms/ml) or immune complexes (100 micrograms/ml) resulted in the inhibition of lysosomal enzyme release and uptake of colloidal radioactive gold of both @entity1 mononuclear phagocytes and @entity788 cells responding to zymosan or immune complexes. In addition those monoclonal antibodies have been found to inhibit @entity6931 -dependent chemiluminescence. Kinetic measurements of chemiluminescence showed a quick inhibition of this activity on both cell types within 1 to 3 minutes. Antibody F(ab')2 fragments behaved analogously to the whole antibodies, whereas monovalent @entity1334 fragments were not able to inhibit any of the mentioned functions. When used alone the monoclonal antibodies were not cytotoxic and did not theirselves trigger release of acid hydrolases, did not alter pinocytosis and did not induce oxidative metabolism.(ABSTRACT TRUNCATED AT 400 WORDS)
