Cytokine production by peripheral blood monocytes during the normal XXXX ovulatory menstrual cycle.

@entity1 @entity650 @entity1356 @entity5 @entity39 @entity310 @entity1193 @entity1192
BACKGROUND: There is considerable evidence that hormone-driven changes resembling an inflammatory response occur in the vascular compartment during the menstrual cycle, and peripheral blood monocytes may be central in the process. We investigated whether there is a cyclical change in intrinsic production of pro-inflammatory cytokines by monocytes in the ovulatory menstrual cycle, and whether there is a circulating factor that influences the pattern of cytokine production in a cyclical manner. METHODS: Monocytes were purified by density-gradient centrifugation followed by countercurrent centrifugal elutriation, from the blood of normal @entity1 (n = 10) pre- and post-ovulation. Monocytes were cultured under basal conditions with bacterial lipopolysaccharide (LPS), and to determine the effects of circulating factors, incubations were also conducted in the presence of autologous serum. Concentrations of @entity1192 , @entity1356 , @entity650 , @entity5 @entity39 factor ( @entity310 and @entity1193 ( @entity1193 ) were measured by sandwich ELISA. RESULTS: The majority of @entity1192 and @entity1356 was cell associated, while the other cytokines were almost entirely secreted. Basal levels of @entity1192 , @entity1356 , and @entity310 were significantly increased following ovulation, while there was no significant change in levels of secretion of @entity650 or @entity1193 . These effects were present in unstimulated cells, suggesting prior activation in vivo. Cytokine production was increased in response to LPS; however, there was no consistent effect of autologous serum. CONCLUSIONS: Intrinsic production of pro-inflammatory cytokines by monocytes is increased following ovulation.
