A psychrotolerant strain of XXXX (MTCC 4822) produces laccase at wide temperature and pH range.

@entity5134 @entity35599 @entity1568 @entity16376 @entity505
A psychrotolerant bacterial strain of @entity5134 , originally isolated from a glacial site in Indian Himalayan Region (IHR), has been investigated for laccase production under different culture conditions. The bacterial strain was found to grow between 4 to 45  C (opt. 25  C) and 3 to 14 pH (opt. 5 pH) on prescribed growth medium, coinciding with production of laccase in laccase producing medium. However, the production of laccase was more consistent toward alkaline pH. Laccase enzyme was partially purified using gel filtration chromatography. The molecular mass of laccase was determined ~53  kDa on native PAGE. The Km and Vmax values were determined to be 0.10  mM and 50.00   M  min(-1), respectively, with @entity16376 . Inoculum size (4.0% v/v at 1.5 O.D.) resulted in significantly higher production of laccase. @entity505 and @entity1568 sources also affected the laccase production significantly. All the @entity505 sources enhanced laccase production, xylose being the best enhancer (P   <   0.01). Among @entity1568 sources, organic sources were found to act as inhibitors (P   <   0.01), and among the in-organic sources only @entity35599 enhanced the laccase production. Low molecular weight organic solvents significantly (P   <   0.01) enhanced laccase production up to 24  h of incubation with a decline in later incubation period. Production of laccase by the psychrotolerant bacterium in wide range of temperature and pH is likely to have inference in biotechnological processes.
