Environmental conditions unexpectedly affect the long-term extent of cell XXXX following an @entity519 episode.

@entity12757 @entity19 @entity40536 @entity3542 @entity2659 @entity519 @entity11495 @entity432 @entity204
Previously we reported delayed cell @entity204 , defined by clear-cut cell loss 60 days after a @entity3542 -induced @entity519 episode. The loss of cells was not apparent two weeks after the treatment, although some changes in cellular appearance were observed at that time. A similar delayed loss of neurons in the hippocampus after @entity519 induced by @entity432 has also been found. In addition, we reported that the amount of @entity12757 induced by the @entity11495 can be reduced by the stress produced by handling and the injection of saline 2 or 24 h before the @entity3542 administration. The degree of methemoglobin formed is directly related to cell @entity204 in certain areas of the brain, including regions within the hippocampus. Considering the many effects that can be produced by chronic and acute stress of several kinds and the length of time during which these effects manifest themselves, we undertook to determine the histologic effects of the stresses of transport on the neuroanatomic effects of @entity11495 administration 60 days post administration. Comparisons were made of the effects of two methods of transport from the laboratory in which the animals (male @entity2659 @entity19 ) were injected with the @entity11495 or saline (Tufts Medical School) to the laboratory in which the histologic evaluations were made (Binghamton University). The animals began their travel several hours after the injections. One transport method was by commuter airline and the other was by automobile. All animals had the same transport from the supplier to the Boston location (truck). Thus, the stress of experimental interest occurred after the @entity3542 administration. Upon arrival at Binghamton University, the animals were housed at the University in their own colony room for 60 days before sacrifice. After sacrifice, sections from their brains were subjected to a number of histologic staining procedures, including PTAH, the @entity40536 method, GFAP, and the standard Nissl procedure. Although special attention was paid to hippocampal areas, changes in cells in the habenulae and the linings of ventricular areas were also prominent. Surprisingly, the @entity3542 treatment before transport to Binghamton offered partial protection against the very substantial and lasting effects of the injections, transport, and handling found in the control animals. Differential effects caused by the two methods of transport were also noted.
