Do endothelial cells belong to the primitive stem leukemic clone in XXXX ? Role of extracellular vesicles.

@entity1 @entity1861 @entity1778 @entity1860
UNASSIGNED: The expression of @entity1861 in hematopoietic stem cells is a well-defined primary event in @entity1860 ( @entity1860 ). Some reports have described the presence of @entity1861 on endothelial cells from @entity1860 @entity1 , suggesting the origin of the disease in a primitive hemangioblastic cell. On the other hand, extracellular vesicles (EVs) released by @entity1778 are involved in the angiogenesis modulation process. In the current work we hypothesized that EVs released from @entity1861 (+) cells may carry inside the oncogene that can be transferred to endothelial cells leading to the expression of both @entity1861 transcript and the oncoprotein. EVs from K562 cells and plasma of newly diagnosed @entity1860 @entity1 were isolated by ultracentrifugation. RT-PCR analysis detected the presence of @entity1861 RNA in the EVs isolated from both K562 cells and plasma of @entity1860 @entity1 . The incorporation of these EVs into endothelial cells was demonstrated by flow cytometry and fluorescence microscopy showed that after 24h of incubation most EVs were incorporated. @entity1861 transcripts were detected in all experiments on endothelial cells incubated with EVs from both sources. The presence of @entity1861 on endothelial cells incubated with Philadelphia(+) EVs was also confirmed by Western blot assays. In summary, endothelial cells acquire @entity1861 RNA and the oncoprotein after incubation with EVs released from Ph(+) positive cells (either from K562 cells or from plasma of newly diagnosed @entity1860 @entity1 ). This results challenge the hypothesis that endothelial cells may be part of the Philadelphia(+) clone in @entity1860 .
