Subthreshold oscillations induced by spinal nerve injury in dissociated muscle and cutaneous afferents of XXXX DRG.

@entity2198 @entity19 @entity128 @entity35 @entity99
Whole cell patch-clamp recordings were obtained from dissociated @entity19 lumbar dorsal root ganglion (DRG) neurons. Recordings were made from control neurons and neurons axotomized by transection of the corresponding spinal nerve 1-2 days prior to dissociation. Medium to large muscle and cutaneous afferent neurons were identified by retrograde transport of True Blue or Fluoro-Gold injected into the corresponding peripheral tissue. Action potentials were classified as non-inflected spikes (A(0)) and inflected spikes (A(inf)). High-frequency, low-amplitude subthreshold membrane potential oscillations were observed in 8% of control A(0) neurons, but their incidence increased to 31% in the @entity128 group. Fifty percent of axotomized muscle afferent A(0) cells displayed oscillations, while 26% of axotomized cutaneous afferents exhibited oscillations. @entity2198 were also observed in a small fraction (4%) of A(inf) neurons on strong depolarization. Their numbers were @entity128 , but the difference was not statistically significant. The oscillations often triggered burst firing in distinct patterns of action potential activity. These results indicate that @entity99 of DRG neurons, previously observed in whole DRG of @entity35 , are present in dissociated DRG neurons of the adult @entity19 . Moreover, these observations indicate that both muscle and cutaneous afferents in the A(beta) size range give rise to @entity99 , with muscle afferents being more prone to develop oscillations.
