Delivery of iPS-NPCs to the XXXX Cavity within a Hyaluronic Acid Matrix Promotes the Differentiation of Transplanted Cells.

@entity64 @entity63 @entity19 @entity1188 @entity717
UNASSIGNED: @entity64 is the leading cause of @entity717 with ~80% being @entity63 . Stem cell transplantation has been shown to improve functional recovery. However, the overall survival and differentiation of these cells is still low. The @entity1188 is an ideal location for transplantation as it is directly adjacent to the highly plastic peri- @entity1188 region. Direct transplantation of cells near the @entity1188 has resulted in low cell viability. Here we deliver neural progenitor cells derived from induce pluripotent stem cells (iPS-NPC) to the @entity1188 of stroked @entity19 encapsulated in a hyaluronic acid hydrogel matrix to protect the cells. To improve the overall viability of transplanted cells, each step of the transplantation process was optimized. Hydrogel mechanics and cell injection parameters were investigated to determine their effects on the inflammatory response of the brain and cell viability, respectively. Using parameters that balanced the desire to keep surgery invasiveness minimal and cell viability high, iPS-NPCs were transplanted to the @entity64 cavity of @entity19 encapsulated in buffer or the hydrogel. While the hydrogel did not promote stem cell survival one week post-transplantation, it did promote differentiation of the neural progenitor cells to neuroblasts.
