Proteases and XXXX gene analysis in 92 giant cell tumors of bone.

@entity1 @entity650 @entity2289 @entity10431 @entity2968 @entity146 @entity5 @entity1777 @entity3 @entity2290
BACKGROUND: @entity2968 ( @entity1777 ) is a @entity5 with a significant tendency to recur locally and rarely to produce pulmonary @entity3 . It is characterized by the presence of multinucleated osteoclast-like giant cells together with mononuclear spindle-shaped cells. Few prognostic markers have been reported to predict the clinical outcome of @entity1777 @entity1 , so is very important to find the factor that can be implicated in its potential @entity146 . @entity1 AND METHODS: Different groups of @entity1777 @entity1 were selected for this study, including @entity1 without evidence of disease and @entity1 who recurred locally or with lung metastasis. The total of 92 @entity5 samples also included the specimens of the local recurrences and the lung @entity3 . By using immunohistochemistry and real-time quantitative polymerase chain reaction techniques, the genetic and proteic analyses were performed on the @entity2290 ( @entity2290 ), its receptor ( @entity10431 ) and its inhibitor ( @entity2289 ), which have been described to be frequently implicated in the process of degradation of the extracellular matrix during the metastatic process. @entity650 ( @entity650 ), a cytokine released by @entity1777 cells, which stimulates resorption of bone, was also analyzed. RESULTS: @entity650 , @entity2290 , @entity10431 and @entity2289 genes were found amplified, respectively, in 7%, 5%, 8% and 12% of total cases (92). In particular, the percentages of amplified genes were higher in the @entity1777 cells that gave rise to @entity3 (12 cases) and in the samples of lung @entity3 (nine cases) compared with the disease-free group of @entity1 (60 cases). CONCLUSIONS: These results suggest a possible association of these factors with a higher biological @entity146 of @entity1777 . Morever, it appears that increased expression of the @entity650 , @entity2290 , @entity10431 and @entity2289 proteins might not depend on mutation of the corresponding genes.
