Studies on the cellular toxicity of XXXX ( @entity3215 ). I. Effect of @entity3215 on microsomal enzymes and on synthesis and turnover of microsomal and cytoplasmic lipids of @entity35 liver- a morphological and biochemical study.

@entity1447 @entity1560 @entity5241 @entity368 @entity14572 @entity653 @entity1110 @entity14774 @entity412 @entity3215 @entity7680 @entity35 @entity413 @entity165
The acute effects of the @entity5241 ( @entity3215 ) mixture ( @entity14774 ) on microsomal enzymes and on synthesis and turnover of microsomal and cytoplasmic lipids of @entity35 liver were investigated. Six daily i.p. injections of 25 and 50 mg @entity5241 /kg body weight resulted in increased liver weight and liver to body weight ratios. When compared to controls @entity5241 treatment resulted in a six-fold increase in amount of @entity1447 . Activities of @entity1110 -cytochrome c reductase, @entity7680 demethylase and @entity14572 diphosphatase were increased whereas @entity413 -6-phosphatase values were decreased by @entity5241 exposure. Analysis of liver homogenate and microsomal fraction revealed an increase in lipid in @entity5241 -exposed animals. Phospholipids, @entity165 and @entity412 were significantly increased after @entity5241 exposure; however, the greatest percentage increase was seen in the @entity412 pool. The finding of an increase in microsomal @entity412 to phospholipid ratios with exposure to @entity5241 is suggestive of an increase in membrane-enclosed lipid (liposomes). Studies with labelled @entity653 indicated that the @entity5241 -induced @entity1560 resulted from increased half life but not increased synthesis of liver lipid moieties. The rate of incorporation of @entity368 into microsomal membrane and albumin was somewhat enhanced in @entity35 exposed to @entity5241 indicative of increased protein synthesis. Morphological studies showed increased occurrence of lipid material, both in cytoplasmic droplets and within rough and smooth-surfaced endoplasmic reticulum. Proliferation of smooth endoplasmic reticulum and flattened Golgi cisternae with no secretion granules containing lipoprotein particles characterized the liver from animals exposed for 6 days. The increase in lipid within membranes of the endoplasmic reticulum together with the flattened Golgi lacking typical secretory vesicles indicates a defect in transport of lipoproteins from the endoplasmic reticulum to the Golgi apparatus and may be the cause of the @entity5241 -induced @entity1560 .
