Antifungal Activity of XXXX In Vitro and In Vivo.

@entity12364 @entity2380 @entity7408 @entity840 @entity281 @entity364 @entity5068 @entity13983 @entity12734
@entity13983 (EA) has been shown to have antioxidant, antibacterial, and anti-inflammatory activities. In Uighur traditional medicine, Euphorbia humifusa Willd is used to treat @entity364 , and recent studies suggest that it is the EA content which is responsible for its therapeutic effect. However, the effects of EA on antifungal activity have not yet been reported. This study aimed to investigate the inhibitory effect of EA on fungal strains both in vitro and in vivo. The minimal inhibitory concentration (MIC) was determined by the National Committee for Clinical Laboratory Standards (M38-A and M27-A2) standard method in vitro. EA had a broad spectrum of antifungal activity, with MICs for all the tested dermatophyte strains between 18.75 and 58.33     g/ml. EA was also active against two Candida strains, with MICs between 25.0 and 75.0     g/ml. It was inactive against @entity7408 . The susceptibility of six species of dermatophytes to EA was comparable with that of the commercial antifungal, @entity2380 . The most sensitive filamentous species was @entity12734 (MIC   =   18.75     g/ml). Studies on the mechanism of action using an HPLC-based assay and an enzyme linked immunosorbent assay showed that EA inhibited @entity12364 biosynthesis and reduced the activity of @entity5068 14a-demethylase P450 (CYP51) in the @entity12734 membrane, respectively. An in vivo test demonstrated that topical administration of EA (4.0 and 8.0   mg/cm(2) ) significantly enhanced the cure rate in a @entity840 @entity281 model of @entity12734 . The results suggest that EA has the potential to be developed as a natural antifungal agent.
