Use of recombinant XXXX stromal cell-derived factor 1a-loaded fibrin/hyaluronic acid hydrogel networks to achieve functional repair of full-thickness @entity291 articular cartilage via homing of chondrogenic progenitor cells.

@entity1 @entity154 @entity571 @entity16 @entity291 @entity130
OBJECTIVE: Articular @entity16 after joint @entity130 seldom heals and often leads to @entity571 . We previously identified a migratory chondrogenic progenitor cell (CPC) population that responds chemotactically to cell death and rapidly repopulates the injured cartilage matrix, which suggests a potential approach for articular cartilage repair. This study was undertaken to determine whether recombinant @entity1 stromal cell-derived factor 1a (rhSDF-1a), a potent CPC chemoattractant, would improve the quality of cartilage regeneration, hypothesizing that increased recruitment of CPCs by rhSDF-1a would promote the formation of cartilage matrix upon chondrogenic induction. METHODS: Full-thickness @entity291 @entity154 were filled with hydrogel, composed of fibrin and hyaluronic acid and containing rhSDF-1a. Cell migration was monitored, followed by chondrogenic induction. Regenerated tissue was evaluated by histology, immunohistochemistry, and scanning electron microscopy. Push-out tests and unconfined compression tests were performed to assess the strength of tissue integration and the mechanical properties of the regenerated cartilage. RESULTS: Use of rhSDF-1a dramatically improved CPC recruitment to the @entity154 at 12 days. After 6 weeks under chondrogenic conditions, cell morphology, proteoglycan density, and the ultrastructure of the repair tissue were all similar to that found in native cartilage. Compared with empty controls, neocartilage generated in rhSDF-1a-containing defects showed significantly greater interfacial strength, and acquired mechanical properties comparable to those of native cartilage. CONCLUSION: This study showed that stimulating local CPC recruitment prior to treatment with chondrogenic factors significantly improves the biochemical and mechanical properties of the cartilage tissue formed in @entity154 . This simple approach may be implemented in vivo as a one-step procedure by staging the release of chemokine and chondrogenic factors from within the hydrogel, which can be achieved using smart drug-delivery systems.
