A @entity164 IV P- XXXX ATPase affects insulin-mediated @entity413 uptake in adipose tissue and skeletal muscle in @entity19 .

@entity28 @entity6 @entity19 @entity71973 @entity34895 @entity164 @entity3240 @entity71974 @entity1995 @entity413 @entity61880
@entity19 carrying two pink-eyed dilution (p) locus heterozygous deletions represent a novel polygenic @entity19 model of @entity164 2 @entity6 associated with @entity28 . @entity71973 , a putative @entity34895 transporter on @entity19 chromosome 7, is a candidate for the phenotype. The phenotype is diet-induced. As a next logical step in the validation and characterization of the model, experiments to analyze metabolic abnormalities associated with these @entity19 were carried out. Results demonstrate that mutants (inheriting the p deletion maternally) heterozygous for @entity71973 are hyperinsulinemic, insulin-resistant and have an altered insulin-stimulated response in peripheral tissues. Adipose tissue and the skeletal muscle are the targets, and @entity3240 -mediated @entity413 uptake is the specific metabolic pathway associated with @entity71973 deletion. Insulin resistance primarily affects the adipose tissue and the skeletal muscle, and the effect in the liver is secondary. Gene expression profiling using microarray and real-time PCR show significant changes in the expression of four genes-- @entity61880 , @entity71974 , @entity3240 and @entity1995 --involved in insulin signaling. The expression of @entity71973 is also significantly altered in the adipose tissue and the soleus muscle. The most striking observation is the loss of @entity71973 expression in the mutants, specifically in the soleus muscle, after eating the high-fat diet for 12 weeks. In conclusion, experiments suggest that the target genes and/or their cognate factors in conjunction with @entity71973 presumably affect the normal translocation and sequestration of @entity3240 in both the target tissues.
