[Effect of short-term XXXX and re-oxygenation on @entity19 peritoneal macrophages in vitro].

@entity1568 @entity19 @entity26 @entity519 @entity3503 @entity4095
Microfluorimetry of single cells could help to analyze their morphology and function state during changes of gas environment. It is very important to have a possibility of the cell visual control during @entity519 and collection of dynamic fluorimetric data in digital form. The effects of short-term @entity4095 decrease were studied. For estimating the effects of @entity519 and reoxygenation we used the @entity19 peritoneal macrophages, which are very sensitive to physical, chemical and regulatory stimuli. A special small chamber for fluorimetric measurements during @entity4095 changes, was developed. The level of active @entity26 forms, intracellular pH, and cell membrane instability were investigated during replacement of air by @entity1568 or @entity3503 (of the basal level decreased to 20% of basic level) and in subsequent reoxygenation. The increase of active @entity26 forms was shown during 30 min of @entity519 and their level continued to rise immediately after reoxygenation. A short-term decrease and subsequent increase of @entity4095 in the medium led to an increase of intracellular pH level. The shifts of measured cell indices were stabilized after 30-40 min of @entity4095 changes thus suggesting a fast comprehension of countermeasure cell mechanisms. No macrophages with membrane disorders were found despite the rise of the active @entity26 forms level during @entity519 and reoxygenation in vitro. There were no significant differences between @entity1568 and @entity3503 used for replacement of air in the medium. The data obtained suggest a high resistance of macrophages against @entity4095 changes and an involvement of the antioxidative mechanisms for cell protection especially during reoxygenation period.
