Cloning and transformation of XXXX ( @entity72438 ) in @entity523 .

@entity3261 @entity36513 @entity1234 @entity523 @entity72438 @entity1521 @entity58455 @entity1865
UNASSIGNED: The @entity523 plant ( @entity523 Mill.) is sensitive to cold, and low field temperatures can result in shortened growth periods and decreased crop yield. Transcription of CRT/DRE-binding factor (CBF) is regulated by @entity72438 ( @entity72438 ). CBF activates many downstream genes that confer cold tolerance on plants. @entity72438 has been used in genetic engineering to improve cold-resistance in several plant species. Here, @entity72438 in a plant expression vector was used to transform a tissue-cultured rhubarb @entity523 variety using @entity3261 . The transgenic and control plants were compared at 4  C for 0, 24, and 72 h. We measured leaf physiological indicators related to cold resistance, including @entity1234 ( @entity1865 ) and @entity1521 ( @entity36513 ) contents, and @entity58455 ( @entity58455 ) and catalase (CAT) activities. At 72 h, the @entity1865 content in transgenic plants was significantly lower than in control plants, indicating a lower membrane lipid injury. The @entity36513 contents and the CAT and @entity58455 activities in the transgenic plants increased significantly compared with those of the control plants. For @entity36513 , the increase continued over the prolonged stress exposure, while CAT and @entity58455 activities reached peak levels at 24 h. These results are consistent with the roles of @entity36513 , CAT, and @entity58455 in defending the integrity of plant cells. Our study not only improves the cold resistance of @entity523 , but also provides the foundation for further research on the role of @entity72438 as a transcription factor in plant cold resistance.
