Drainage of molecules from subarachnoid space to spinal nerve roots and peripheral nerve of the rat. A study based on Evans blue-albumin and XXXX as tracers.

@entity1897 @entity7395 @entity3740 @entity21
The purpose of the present investigation was to find out if a compound injected into the spinal subarachnoid space, after having entered ventral and dorsal nerve roots, can be traced to the @entity1897 . This would indicate a centrifugal movement of substances from the cerebrospinal fluid along nerves; one route of drainage of cerebrospinal fluid which in the past has been widely discussed. In vivo studies were made using Evans blue-albumin and @entity7395 as tracers. Evans blue-albumin is macromolecular in size and emits a red fluorescence after exposure to ultraviolet light. @entity3740 ions are small and easily visible in the electron microscope. The tracers were injected into the cervical subarachnoid space and 15 min to 24 h later samples from roots, dorsal root @entity21 , proximal part of spinal nerves and the median nerves were taken and further processed for detection of tracers. Fluorescence microscopy from samples removed 15 min and 24 h after the injection of Evans blue-albumin showed a red fluorescence of low intensity in the endoneurium of nerve roots, @entity21 and proximal spinal nerve. After 24 h also the median nerve elicited some fluorescence. The sheaths around these structures were also fluorescent. @entity3740 was detected between cell layers of the nerve root sheath as well as inside the nerve root parenchyma. In about 50% of the samples from dorsal root @entity21 extracellular @entity3740 was found in the capsule. The tracer was also found in the epineurium of 50% of the spinal nerves and occasionally in the perineurium.(ABSTRACT TRUNCATED AT 250 WORDS)
