Expression and activities of three inducible enzymes in the healing of XXXX in rats.

@entity1506 @entity1066 @entity22456 @entity842 @entity1446 @entity4665 @entity1543 @entity2275
AIM: To explore the roles of @entity842 synthase (NOS), @entity1446 oxygenase (HO) and cyclooxygenase (COX) in gastric ulceration and to investigate the relationships of the expression and activities of these enzymes at different stages of gastric ulceration. METHODS: @entity2275 (kissing ulcers) were induced by luminal application of @entity1543 . Gastric tissue samples were obtained from the @entity1066 base, @entity1066 margin, and non-ulcerated area around the @entity1066 margin at different time intervals after @entity1066 induction. The mRNA expression and protein levels of inducible and constitutive isoforms of NOS, HO and COX were analyzed with RT-PCR and Western blotting methods. The activities of the total NOS, @entity1506 ( @entity1506 ), HO, and COX were also determined. RESULTS: Differential expression of inducible @entity1506 , @entity22456 and @entity4665 and enzyme activities of NOS, HO and COX were found in the gastric @entity1066 base. High @entity1506 expression and activity were observed on day 1 to day 3 in severely inflamed @entity1066 tissues. Maximum expressions of @entity22456 and @entity4665 and enzyme activities of HO and COX lagged behind that of @entity1506 , and remained at high levels during the healing phase. CONCLUSION: The expression and activities of @entity1506 , @entity22456 and @entity4665 are found to be correlated to different stages of gastric ulceration. @entity1506 may contribute to @entity1066 formation while @entity22456 and @entity4665 may promote @entity1066 .
