Nuclear translocation of the glucocorticoid receptor in fibroblasts of asthmatic XXXX with @entity1412 insensitive to glucocorticoid treatment.

@entity1 @entity565 @entity1097 @entity619 @entity4184 @entity1412
BACKGROUND: @entity1412 ( @entity1412 ) is treated with topical glucocorticoids (GC). Some @entity1 require endoscopic nasal surgery because GC treatment is ineffective. To exert its function, the GC needs to bind with the GC receptor (GR) and the GC-GR complex moves to the cell nucleus. Our aim was to establish whether the poor response to GC is due to an alteration in the translocation of the GR to the nucleus. METHODS: We performed nasal fibroblast cell cultures from seven healthy controls and 12 @entity1 with @entity1412 and @entity565 . Fibroblasts were incubated with @entity4184 or @entity619 (10(-7) M) for different times (30 min to 4 h) and GR translocation was analyzed by immunocytochemistry. RESULTS: Both GC induced GR translocation in every group, doubling its concentration in the cell nucleus (30 min) compared to baseline. There were no differences in GR translocation between controls and @entity1 , nor differences related to the severity of @entity565 or intolerance to non-steroidal anti-inflammatory drugs (NSAIDs). @entity1097 subjects showed a decrease in GR translocation with @entity4184 (1 h, 3 h and 4 h, P<0.05) and @entity619 (30 min and 2 h, P<.05). CONCLUSIONS: The insensitivity to GC treatment does not appear to be due to an alteration in GR translocation to the nucleus. Neither does the severity of @entity565 or intolerance to NSAIDs appear to alter GR translocation. The association between atopy and the alteration in GR function deserves further investigation.
