Pharmacological characterization of @entity1919 receptors mediating gene regulation in XXXX .

@entity268 @entity4328 @entity10995 @entity1919
Extracellular molecules regulate gene expression in eucaryotes. Exogenous @entity1919 ( @entity268 ) affects the expression of a large number of developmentally regulated genes in @entity4328 . Here, we determine the specificity of the receptor(s) which mediates gene expression by using analogs of @entity268 . The order of potency with which these analogs affect the expression of specific genes is consistent with the specificity of their binding to a cell surface receptor and is distinct from their affinity for intracellular @entity268 -dependent protein kinase. Dose-response curves with @entity268 and @entity10995 , a nonhydrolyzable analog, revealed that the requirement for high concentrations of exogenous @entity268 for regulating gene expression is due to the rapid degradation of @entity268 by phosphodiesterase. The addition of low concentrations of @entity268 (100 nM) or analogs in pulses also regulates gene expression. Both the genes that are positively regulated by exogenous @entity268 and the discoidin gene, which is negatively regulated, respond to @entity268 analogs to the same degree. Genes expressed in prespore or prestalk cells are also similarly regulated. These data suggest that the effects are mediated through the same receptor. The specificity of this receptor is indistinguishable from that of the well-characterized cell surface @entity268 receptor.
