BACKGROUND: Personal protective equipment (PPE) compliance is important to reduce the rate of transmission of virulent pathogens to health care workers. Medical officer compliance with PPE protocol for COVID-19 was audited in a regional hospital in Australia early in the pandemic response. METHODS: Compliance was assessed based on the order and technique of donning and doffing PPE, with medical officers from multiple departments and levels of seniority audited. RESULTS: Average compliance from all participants was 58.61% with donning and 68.84% with doffing. CONCLUSION: Medical Officer compliance with PPE donning and doffing was poor and additional training was required.