The spread of COVID-19 has brought about huge losses around the world. This study aims to investigate the applicability of PHMB used for developing antiviral spandex clothing against coronavirus. PHMB was qualitatively determined on the surface of spandex fabrics by using BPB. The antiviral analysis shows that the PHMB-treated spandex fabric can kill 99% of the coronavirus within 2 h of contact, which suggests that the spandex fabric treated with PHMB could be used for developing antiviral clothing against coronaviruses for containing the transmission of COVID-19 in high-risk places. Furthermore, PHMB-treated spandex fabrics were shown excellent antibacterial activity against gram-positive S. aureus and gram-negative K. pneumoniae. The hand feel properties of Spandex fabric were not significantly affected by the PHMB coating in addition to the wrinkle recovery, which was obviously improved after PHMB coating.