The COVID-19 pandemic has impacted the entire world, causing a great number of mortality of humans and affecting the economy, while conservation efforts are finally recognized to prevent further pandemics. The wildlife rehabilitation centers (WRCs) play a relevant role in animal welfare; nevertheless, they also represent an imminent risk of pathogen transmission between humans-to-animals and between animals. Moreover, WRCs could spread pathogens into natural habitats through the reintroduction of infectious individuals. These biosafety concerns at WRCs may increase as the economic and social impact of the COVID-19 extends. We explored the current situation of Latin American WRCs under the COVID-19 pandemic to determine the feasibility of SARS-CoV-2 introduction, amplification, and spread within these institutions. We surveyed WRCs from eight Latin American countries. We found that pandemic is affecting these institutions in many aspects: workers with symptoms compatible with COVID-19, reduced economic resources, and lack of information and support from governmental authorities. These have forced WRCs to reduce the workforce, veterinary visits, and animal food rations and to increase the number of animals released. This scenario generates a risky environment for the transmission of SARS-CoV-2, especially for felids, mustelids, and non-human primates. Therefore, it is imperative to respect quarantine periods, monitor incoming patients, increase biosecurity measures, develop and apply guidelines and recommendations for the protection of personnel and biosafety of enclosures and instruments. It is of utmost importance the proper and safer reintroduction of recovered wildlife.