First step to keeping donor hearts viable up to 24 hours after removal
Fifty-six years after the first human-to-human heart transplantation, more than 5,000 hearts are transplanted each year around the world.

This number is far from enough to give a new heart to everyone who needs one, with up to 50,000 people needing one at any time.

Depending on how ill they are, as well as physiological and logistical factors, candidates may wait for years.

A bottleneck is the availability of suitable donor hearts, partly due to the very short time window for transplantation once the heart is removed from the deceased donor.

The current gold standard for preserving donor hearts is cold static storage (CSS), where hearts are kept on ice until transplantation.

Transplantation is most successful when CSS lasts less than six hours, before the heart or its blood vessels undergo damage.

Periods up to 12 hours are sometimes possible, but require mechanical life support such as extracorporeal membrane oxygenation (ECMO), for several days on the recipient.

Prolonging the storage period beyond six hours without the need for ECMO would thus be a medical breakthrough.

Now, researchers publishing in the journal Frontiers in Cardiovascular Medicine have shown it’s possible to keep transplanted pig hearts alive outside the body for more than 24 hours using a process called normothermic ex-vivo heart perfusion (NEHP).

"If translated to humans, this would be a major improvement to the six-hour-long time window in standard clinical practice,” said Emeritus Professor Dr Robert Bartlett, who heads the Extracorporeal Life Support Laboratory at the University of Michigan Medical School at Ann Arbor in the United States.

Keep it pumping

NEHP means that hearts, once removed from their donor, are kept in a partly physiological state at room temperature by pumping oxygenated, nutrient-rich fluid (perfusate) derived from blood plasma through them until transplantation.

Drugs and tissue-repairing stem cells can be delivered to the heart through the perfusate.

Currently, there is only variant of NEHP approved for clinical use by the US Food and Drug Administration (FDA), and like CSS, it is limited to six hours.

The Extracorporeal Life Support Laboratory has worked over the past seven years on steadily extending the shelf-life of donor hearts through improvements to NEHP.

Their previous experiments have shown that a critical step is to filter the perfusate to remove all molecules smaller than 26 kilodalton.

Without this, for unknown reasons, hearts quickly become unusable for transplantation.

Emeritus Prof Bartlett and his colleagues reported keeping the hearts of 30 immature and 10 juvenile pigs alive for various periods with experimental variants of NEHP.

The perfusate for all the donor hearts was a solution of blood plasma and packed red blood cells (from other healthy pigs), electrolytes, glucose and antibiotics.

It was pumped through the heart at a mean rate of 0.7 milliliter per minute per gram heart weight, and replaced every 60 minutes.

The researchers then compared the effects between three variants: NEHP with haemofiltration to continuously purify the perfusate and remove toxins (10 immature pig hearts); NEHP where the plasma component in the perfusate was exchanged continuously (five immature hearts); and control NEHP without modifications (15 immature hearts).

To test these methods on larger hearts, they also used NEPH with haemofiltration on five hearts from juvenile pigs, and NEPH with an additional modification (intermittent left atrial perfusion or iLA) on a further seven hearts from juveniles, to monitor heart function.

In iLA, a fixed volume of blood is injected into the left atrium at regular intervals, to test its continued power to eject this blood.

The authors monitored the health of the preserved hearts in real time by visually checking their contractility, rhythm, colour and oedema, and by measuring the concentration of lactate (a byproduct of cellular damage) every hour.

Each heart was maintained until it went into asystole or arrhythmia, showed minimal systolic blood pressure in the left ventricle, or showed elevated lactate concentrations for at least two hours.

Promising methods

All the control hearts died between 10 and 24 hours after removal from the pig donor, while all hearts that had been maintained with modifications to standard NEPH survived for the full 24 hours.

The authors conclude that haemofiltration, plasma exchange and iLA are major improvements that enable the routine preservation of hearts to beyond one day.

Which of the three methods is better can’t be answered yet.

"I think the major difference will be when we extend our experiments beyond 24 hours, where perhaps plasma exchange is better as larger toxins can be removed.

"iLA also seems a major improvement, as in principle, it would allow NEPH to be used on hearts that have suffered injuries or have borderline function in the donor,” said Emeritus Prof Bartlett.

"This work could ultimately increase the donor pool.

"First, by extending the preservation time, thus overcoming limitations due to logistics.

"Second, by giving an objective assessment of the viability of each potential donor heart, to reduce the number that currently aren't used when it’s not clear how well they function inside the donor,” said study corresponding author Dr Alvaro Rojas-Pena.

"The major challenge for clinical application will be the validation of the methods in humans.

"To this end, we have started to work with human hearts rejected for transplantation,” he said.
Wellness,Heart,heart transplant,organ donation
Pig hearts kept alive outside the body for more than a day offers hope for many humans needing a heart transplant.