On 10 September 1968 Annadurai travelled to New York for medical treatment and he was operated for Cancer in the gullet at the Memorial Sloan–Kettering Cancer Center. He returned to Chennai in November and continued to address several official functions against medical advice. His health deteriorated further and he died on 3 February 1969. His cancer was attributed to his habit of chewing tobacco. His funeral had the highest number of attendees until then, as registered with "The Guinness Book of Records". An estimated 15 million people attended it. His remains were buried in the northern end of Marina Beach, which is now called Anna Memorial.

మద్రాసు హిందీ వ్యతిరేక ఉద్యమం (1965)
Legacy
పెరియార్‌తో విభేదాలు, డిఎంకె ఆవిర్భావం
మరణం